LOCUS       DAOOLN010000001        10229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_7494_length_10229_cov_3.325241, whole genome shotgun sequence.
ACCESSION   DAOOLN010000001 DAOOLN010000000
VERSION     DAOOLN010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 10229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10229
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7494_length_10229_cov_3.325241"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PLW80_00005"
     CDS             <1..608
                     /locus_tag="PLW80_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HPB64909.1"
                     /translation="GERGLRQGESAYIALREGRESMSPRFGFSQDFSLGVEPAFNDDG
                     ELAPERLEIVSGGAIANTVVCSRTAKQYGVPANGAGEDESVRSISMDAGDLAESDALK
                     AIGTGVYVSNFHYLNWSDVQAARVTGMTRFACLWVENGEIVGPIKDMRWDESLYNMLG
                     AKLEAVTKERHLLVASETYERRQVGGSLLPGILVDGLSFTL"
     gene            complement(697..903)
                     /locus_tag="PLW80_00010"
     CDS             complement(697..903)
                     /locus_tag="PLW80_00010"
                     /inference="COORDINATES: protein motif:HMM:NF016028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HPB64910.1"
                     /translation="MDQDRIEAIETKLAYLEGSVSDIDALVYEYGRRTERLEEAVREL
                     AKRLGELGDDKAATPPASERPPHY"
     gene            1040..1612
                     /locus_tag="PLW80_00015"
     CDS             1040..1612
                     /locus_tag="PLW80_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64911.1"
                     /translation="MRNTIIVALAALVSISAAYSQEADEGTIDDREPALSVSVGPEVA
                     ASLTGAFAGVQAQFAWRPGFFGLVAGVRADVYLSQPDVYVIPSAGVRLGWFELTGGAA
                     FKVYDAPEPPNYIEASTEGASPFLRAGLDVPIGPISVGLGVRLMVADTYTESTAETVG
                     DAIGEGIAAAIVAVFSIFQLDLGLSYRLTF"
     gene            complement(1626..3539)
                     /locus_tag="PLW80_00020"
     CDS             complement(1626..3539)
                     /locus_tag="PLW80_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPB64912.1"
                     /translation="MNILSLDGVSKTLIGTPLFKDVSLGIEEGERLGLVGRNGAGKST
                     FLRVLSGALEPDAGSIARRRGLRVSVLPQQPAAKPGTKLSEFLYEGDSELVRLVRDYE
                     AAVSGKTGATDRALEALYARMETEGGFLLERRYASLCSEFGLPDIGAPMDGMSGGMVK
                     KAALARTLADSSDLVLLDEPTNHLDLDAIELLERKLGSAAFAFVVVTHDRAFLDAVAG
                     RILEIDRSSVFSYPGDYSAFVAMKAERWNALEKADSRRETILKIEMKWLMRGARARAT
                     KSERRKELIRGMQDSALERPTPMGAFSSKSRRLGKKVLELRGAAKRYGGRLVFEPFSY
                     EFVKGDRIGVVGPNGSGKTTLLGLIAGRLAPDEGSVERGVNTVCSYYGQTAEGLPMDA
                     RMLDFIRSRAELTAMGDGSLLDPERLLERFLFDRTMHDQRLSTLSGGELRRLQLVSVL
                     AEHPNLLILDEPTNDLDIDTIELLEDYVESFDGCVIVVSHDRAFLDGVTSTTIAIDGS
                     GGASLYPYAYGAYREYLEAEAEAEAKGEAERVAAERRQPGVGQQRGRDRSDRSDRAGE
                     PRKPTWAERKEYEGILDEIQALEDEKSSLEALFSSGASGDDLERAGRRYAELCPLIDS
                     RTARWEELAALIG"
     gene            3636..4661
                     /locus_tag="PLW80_00025"
     CDS             3636..4661
                     /locus_tag="PLW80_00025"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HPB64913.1"
                     /translation="MSHRTDPAAPRADYSDLTPELALSAVGEAFGIEPDGSFFAYPSY
                     ANRVYGLRADDGTEYVAKFYRPGRWTPEAILEEHAFVAELEAAEIPAVAPLATPDGDT
                     LPVLAIEGMAGETEYPFALFPKRGGRLFDAERDEDWLRLGALAGRIHAVGQARPFSLR
                     VGIGPGLAGRYADEIVDGGALDPDAVDRVAPLLRRSAALVDEALARAPAPIRLHGDFH
                     RGNVLDRADGGLLAIDFDDASYGPAVQDLWLLLPGPASECRRELGLIVEGYEGFMPFD
                     SRSLALVEPLRLLRMVHFIAWQARQRSDRGFAERWGDWGSKAYWMERALDLADQIERV
                     ESAGPGF"
     gene            complement(4641..6248)
                     /locus_tag="PLW80_00030"
     CDS             complement(4641..6248)
                     /locus_tag="PLW80_00030"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB64914.1"
                     /translation="MPVNRSIRSSVPFQFAAVTLFFTALVALLESLLVVRAFSSIELN
                     GMRDRVAQFDAFLGRERESLANLAKSYAEWGDSYSFMSTRDPAYLENNYNADWIGSQG
                     LDYVLIVDDDGRVVWDSGSSAPAFFPGSAGAWLYRSPLLFSPEYGSRPSASVRSLVEL
                     DGEPVLYASWPITDDLVTVAPRGVLVFARRLDPDALAEYVAGGEFSMGWRSARGSGTL
                     PEPGPVKVSDGGRAYLRGAEPVRDAAGAIIGYWEFSQERNWIRTIRQMVAWFALIGAA
                     GGLGIYAVLHRRVRRLIVVPLMAIKDRLGGFSRTLEALEPLPPFGYEELDELANHVYA
                     LTERVCSQTMELDRLASTDGLTGLPNRRSLEKTMDEFRRRRATGKLGVDCRSTSKRGT
                     IAFGMIDVDFFKNYNDLYGHAGGDATLRAIGLVIRECVKRPDDLPSRYGGEEFAVILP
                     DTDEEGAVAVLERVRQAIEALELPHAGSSAANVVTVSCGAAALRPDGPDGDLDQIMEM
                     ADKALYAAKAFGRNRVIAYSSLKPGAS"
     gene            6366..8144
                     /locus_tag="PLW80_00035"
     CDS             6366..8144
                     /locus_tag="PLW80_00035"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPB64915.1"
                     /translation="MDYSELSMHPDLAKGVQDAGYVTCMPVQAAVFPHAFAGEDIYAQ
                     SQTGTGKTAAFLVSIFQRIMSDSASRHRKVLILAPTRELAVQIEEEAKTLGKHLPISI
                     GSFYGGVAYGPQLDMLRDNVQMLIGTPGRIIDLVQQGKMLLRDVGFLVVDEADRMFDM
                     GFIDDLRKLLRYLPQPKERQTFLFSATLNFRIKNLAWEYMDEPKEIVIEAETVTVDLV
                     TQELYHVGSDEKFRVLLGILARDKPSSALIFCNQKFMAEEISRRLGINGYECEYIMGD
                     LPQAKRLQVIERLKAGKLSMLVATDVAARGLDVEALDLVVNYDVPMDPESYVHRIGRT
                     ARAGATGKAVTLACEKFVYGLPGIEKYIEAKIPVCPVTEELFGDDKSAGIRFGHSHDR
                     ARPGSDRGAGHGGSSYRGGGQGAGRGYGDRGPRRGPSDRAQPQRGSAPRPKQASQAGG
                     PQAGAPRPAHQGGRDPYAISSEERMRRYKDKYAGGQADRGQEPGRPADGQQRQSQQRQ
                     SQQRQGQSRRQGQQPAQGQAQRQQPRQAQRPASQGTKPQASKPQGTGPRAQGSMPQAN
                     NAGRPAKPGLIDRIKRAFGLGKRADD"
     gene            8160..9278
                     /locus_tag="PLW80_00040"
     CDS             8160..9278
                     /locus_tag="PLW80_00040"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPB64916.1"
                     /translation="MNDRGDFRRPRLDPEQLFSPFDRALGTLHERLFGRYRLVTVAAS
                     ILIYSAAVLSTGASLGVSTNYFVLLPVICASMGYGLFAGVVAGIVALPGNLILYRALG
                     HPEFAPASKAMAELSGILVGTVLGYLSDYHRKLEVERYLRKETESELRRALRDREALF
                     REVHHRVKNNLNLIKSIIGLQARRSADPAFKDAAAMLTGRIMSISFVHERLYRTSELS
                     AVAVDEYLKDLVNAVVMAAGGSDTCPTVAFDLSRHDASMDVAVPLGLIVNELVTNSIK
                     HGQPRRGRPLAMEIRLSTDGDELSLSVRDDGVGLPGVDEGRRLAIEEAVLRFPGSLGL
                     TLLDLMASQLGGKAAFSRSDGWTEFNLIFRDAAAAERP"
     gene            complement(9288..9605)
                     /locus_tag="PLW80_00045"
     CDS             complement(9288..9605)
                     /locus_tag="PLW80_00045"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HPB64917.1"
                     /translation="MQLSLVRPHEPFIIRSVSVGREVGRRLADMGCTEGTEGVIVRRG
                     GFGGPMQVRIHGYDLLIRRDEAARIDVETVGPVRGPGRGMGRGRGRGGRWWAKSGEAE
                     DLS"
     gene            9730..>10229
                     /locus_tag="PLW80_00050"
     CDS             9730..>10229
                     /locus_tag="PLW80_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPB64918.1"
                     /translation="MRRFLKVVIVVSGLALLVTLAASIALASRMRDGARSPDARQGGQ
                     ALFHILAVVPASDAERYYARAVEAMTRTARKRGAVLQTLEYDQDNAPSAIARQLRLAA
                     ELEPDGVVVSAPLDPLVTEAVDTLSKRGVPVVALETDLPGSARAAFIGTNPYSVGVLA
                     AEAVAE"
BASE COUNT         1707 a         3509 c         3362 g         1651 t
ORIGIN      
        1 tcggcgagcg cggtctcagg cagggagaga gcgcctacat cgcgctgcgc gagggccgcg
       61 agagcatgtc gccgagattc ggcttcagcc aggacttctc gctcggcgtg gagccggcct
      121 tcaacgacga cggcgagctg gctcccgagc ggctcgagat cgtctccggc ggcgcgatag
      181 ccaacacggt cgtctgctcg aggacagcca agcagtacgg cgtcccggcc aacggcgccg
      241 gcgaggacga gagcgtacgt tcgatctcga tggacgcggg cgacctggcc gagtccgacg
      301 cgctcaaggc catcggcacc ggcgtctacg tgtcgaactt ccactacctg aattggagcg
      361 atgtgcaggc ggctcgcgtc accggcatga cccgcttcgc ctgcctctgg gtggagaacg
      421 gcgagatcgt cggcccgatc aaggacatgc gctgggacga gtctctgtac aacatgctcg
      481 gcgccaagct cgaggccgtg accaaggagc gccacctgct cgtggcctcg gagacctacg
      541 agcgcaggca ggtcggcggc agcctgctgc cgggcatcct ggtcgacggg ctgtcgttca
      601 cgctgtaagg acggaggcgg ccgccctccc ggccgcctcc ttttaatagc ccctcgtccc
      661 cctggacgag gggcggttct taccctgggg cacggctcag taatgtggcg gccgttcaga
      721 ggccggcggc gttgcggctt tatcgtcgcc cagctcgcca agacgtttag ccagctcgcg
      781 caccgcctcc tcgagcctct cggtacgccg gccgtattcg tagacgagcg cgtctatatc
      841 gcttaccgag ccttcgaggt acgcgagctt cgtctctatg gcctctatcc tgtcctggtc
      901 catcgtcatc ctccggccgc cgaggctggg tcgcttctaa ctcggcgcga tatagtatac
      961 ggtatcggca tcgcgtccgt ccgcgccgcg gaccgcgcca ttgaggcgag catagcaaac
     1021 cggcgccagg agcagcatca tgagaaacac tatcatcgta gcgctggcgg cgctcgtatc
     1081 catctcggcg gcctactcgc aagaggcgga cgaaggaacc atagacgatc gcgagccggc
     1141 tctgtccgtc tcggtcggcc cggaggtcgc ggccagcctg accggcgcct tcgcgggcgt
     1201 tcaggcccag ttcgcctggc ggcccggctt cttcggcctc gtcgcggggg tccgtgcgga
     1261 cgtgtacctg agccagccgg acgtttacgt gattccgagc gccggcgtac ggctcggctg
     1321 gttcgagctc acgggcggcg ccgccttcaa ggtctacgac gccccggagc ccccaaacta
     1381 catcgaggcg agcaccgagg gggcctcccc gttccttcgg gccgggctgg acgtgcctat
     1441 cggccccatc tccgtaggcc tgggcgtgag actcatggtg gccgacacgt atacggagag
     1501 cacggccgag acggtcggcg atgcgatcgg cgagggcatc gcagccgcga tcgtcgccgt
     1561 cttcagtatc ttccaactgg atctcggcct gagctacagg ctgacgttct agcggcgacg
     1621 acccgctagc ctatcagggc cgccagctcc tcccagcggg cggtccgcga gtcgatgagc
     1681 gggcatagct cggcgtagcg ccggcccgcc cgctccaagt cgtcgccgga ggcgcctgac
     1741 gagaatagcg cctcgagcga tgacttctcg tcctccagcg cctggatctc gtccaagata
     1801 ccctcgtatt ccttgcgctc ggcccaggtg ggcttcctcg gttcgccagc gcgatcggat
     1861 cgatcggatc gatcgcggcc gcgctgttgg ccgacgcctg gctgccgcct ctcggcggcg
     1921 acgcgctcgg cctcgccctt cgcctcggct tcggcttcgg cttcaaggta ctcgcggtag
     1981 gccccgtagg cgtacgggta gagcgacgcg ccgccggagc cgtcgatcgc tatggtcgtc
     2041 gacgtgaccc cgtccaggaa ggcccggtcg tgggacacga cgatcacgca gccgtcgaac
     2101 gactcgacat agtcctcgag cagctcgatc gtgtcgatat ccaggtcgtt ggtcggctcg
     2161 tccaggatca gcaggttcgg gtgctcggcc aggaccgaca ccagctggag tcgcctgagc
     2221 tcgccgcctg atagcgtgga caagcgctgg tcgtgcatcg tgcggtcgaa caggaagcgc
     2281 tcgagcaggc gctcggggtc gaggaggctg ccgtcgccca tggccgtcag ctcggcgcga
     2341 gagcggatga agtccagcat cctggcgtcc atgggcaggc cctcggcggt ctggccgtag
     2401 tacgagcaga ccgtgttgac accgcgctcc accgagccct cgtcgggcgc cagccggccg
     2461 gctatcaggc cgagcagcgt cgtcttgccg gagccgttcg gaccgaccac gccgatgcgg
     2521 tcgcccttga cgaattcgta tgagaacggc tcgaagacga gccggccgcc atagcgcttg
     2581 gccgctcccc tgagctcgag caccttcttg ccgagccgtc tggacttcga cgagaaggcg
     2641 cccatcggcg tcgggcgctc gagagcggag tcctgcatgc ctcggatcag ctccttgcgc
     2701 cgctcgctct tggtcgcgcg ggccctggcg ccgcgcatca gccacttcat ctcgatcttc
     2761 aggatggtct cgcgcctgga gtcggccttt tcgagcgcgt tccagcgctc cgccttcatc
     2821 gcgacgaagg cggaatagtc gcccgggtag gagaagaccg acgaccggtc tatctcgagg
     2881 atgcgaccgg cgacggcgtc caggaaggcg cggtcatggg tgacgacgac gaaggcgaac
     2941 gccgccgagc cgagtttacg ctcgaggagc tcgatcgcgt ccaggtccag gtggttcgtc
     3001 ggctcgtcga gcagaaccag gtcggacgag tcggccagcg tccgggccag ggcggccttc
     3061 ttgaccatac cgccggacat gccgtccatc ggggcgccta tgtcgggcag gccgaattcg
     3121 gagcacagcg acgcgtagcg ccgctcgagg aggaagccgc cctcggtctc catcctggcg
     3181 tacagcgcct caagggcgcg atccgtggcg cccgtttttc cggataccgc tgcctcgtag
     3241 tcgcgtacca ggcggaccag ctccgagtcg ccctcgtaca ggaactccga cagcttggtc
     3301 ccgggcttgg ccgccggctg ttgcggcagg accgataccc tgaggccgcg cctgcgggct
     3361 atggagccgg cgtcgggctc gagagccccg gacagcacgc gtaggaaggt ggacttgccg
     3421 gcgccgttgc gcccgacgag gccgaggcgc tcgccctcct cgatgccgag cgagacgtcc
     3481 ttgaacagcg gggtgccgat gagcgtcttg gagacgccgt cgagggatag aatattcatg
     3541 cgtagccgga ctatagcccg tttcggcccg cagtgtcacc tcgccgcgcg gcccggggcg
     3601 atactcccct cccccggccg tgctatactc ggccgatgag ccaccgcacc gacccggccg
     3661 cgccgcgcgc ggactacagc gacctgaccc cggagctggc cctatccgcc gtcggcgagg
     3721 ccttcgggat agagccggac ggctcgttct tcgcgtatcc gagctacgcc aaccgagtct
     3781 acgggctccg cgccgacgac gggaccgagt acgtggccaa attctaccgc cccggccgct
     3841 ggacgccgga ggccatcctc gaggagcacg ccttcgtcgc ggagctggag gctgccgaga
     3901 taccggccgt ggcgccccta gcgacgccgg acggcgacac gctaccggtg ctggctatcg
     3961 agggcatggc cggagagacc gaatacccgt tcgcgctgtt tccgaaacgc ggcggccgcc
     4021 tcttcgacgc tgagcgcgac gaggactggc tcaggctcgg cgccctcgcg ggccgcatcc
     4081 acgcggtcgg ccaggcgcgc ccgttctcgc tgcgcgtcgg catcggtcca ggcctggccg
     4141 gccgttacgc cgacgagata gtcgacggcg gcgccctcga tcccgacgcc gtcgaccgcg
     4201 tcgcgccgct cctcaggcgc tcggccgccc tcgtcgacga ggcgctcgcc cgggctcccg
     4261 ccccgatacg gctccacggc gacttccacc ggggcaacgt gctcgatcgc gccgacggcg
     4321 ggctcctggc gatcgacttc gacgacgcct cctacgggcc tgcggtccaa gacctgtggc
     4381 tcttgctacc gggcccggcc agcgagtgcc gccgggagct cggcctgatc gtcgagggct
     4441 acgaaggctt catgcccttc gacagccgat cgctggccct cgtcgagccg ctcaggctac
     4501 tcaggatggt ccacttcatc gcgtggcagg ccaggcaacg ttccgaccgc ggcttcgccg
     4561 aacgctgggg cgactggggc tccaaggcct actggatgga gcgagcgctc gacctggccg
     4621 accagatcga gcgggtcgag tcagctggcc ccgggtttta gggacgaata ggcgatgacg
     4681 cggttacgcc cgaacgcctt ggccgcgtac agcgccttgt cggccatctc catgatctgg
     4741 tcaaggtcgc cgtcggggcc gtctggccgt agggccgccg cgccgcagct gacggtcacg
     4801 acgttcgccg cgctcgaccc agcgtgcggc agctcgagcg cctctatcgc ctgtctgacg
     4861 cgctcgagca ccgccacggc gccttcctcg tcggtatcgg gcaggatcac ggcgaactcc
     4921 tcgccaccgt agcggctcgg caggtcgtcc ggccgcttga cgcattccct gatcacgagc
     4981 ccgatcgccc tgagcgtcgc gtcgccgccg gcgtggccgt acagatcgtt gtagttcttg
     5041 aagaagtcga cgtctatcat gccgaaggct atcgtgccgc gcttgctcgt cgagcggcag
     5101 tcgacgccga gcttacccgt ggcgcgccta cgcctgaatt cgtccatggt cttctcgagg
     5161 ctgcgccggt tcggcaggcc cgtcaggccg tcggtgctcg ccagcctatc gagctccatg
     5221 gtctgcgagc atacccgctc cgtgagcgcg taaacgtggt tcgccagctc gtccagctcc
     5281 tcgtacccga acggcggtaa gggttcgagc gcctccagag tccgcgagaa gcctccgagg
     5341 cgatccttga tggccatcag agggacgacg atcaggcgcc tgacgcgtct atgcagcacg
     5401 gcgtagatgc cgagtccgcc ggccgcgccg atcagcgcga accacgcgac catctggcgt
     5461 atcgtccgta tccagttccg ctcctggctg aattcccagt agccgattat ggcgccggct
     5521 gcgtcgcgca ccggctcggc gcctcggaga taggcccgcc ccccgtcgct gactttgacg
     5581 gggccaggct ccggtagcgt tccagagccg cgggccgagc gccatcccat gctgaactcg
     5641 ccgccggcga cgtactcggc gagcgcgtcc ggatcgagcc gccgagcgaa gacgaggacc
     5701 ccgcgcggcg cgaccgtgac caggtcgtcc gtgatgggcc aggaggcgta caggacgggc
     5761 tcgccatcga gctcgacgag gctccggacg gacgccgacg gtctggagcc gtattccggg
     5821 gagaatagca ggggcgagcg atagagccac gcgcccgccg agccagggaa aaacgcgggc
     5881 gccgaggagc ccgagtccca gacgacgcgc ccgtcgtcat cgacgatgag gacatagtcc
     5941 aggccctgcg aaccgatcca gtcggcgttg tagttattct ccaggtacgc cgggtcgcgc
     6001 gtcgacatga acgagtagct gtcgccccac tcggcgtatg acttggcgag gttcgccagg
     6061 gactcgcgct cccggccgag gaaggcgtcg aactgggcca cgcgatcccg catgccgttc
     6121 agctcgatcg acgagaaggc ccgtacgacg agtagcgact ccaatagcgc caccaaggcg
     6181 gtgaagaaca gcgttaccgc cgcgaactgg aatggcaccg acgacctgat cgatctgttt
     6241 accggcatgg ccatggttcc agtataggcg acgcccgaac cgaaataaag cttcggcgag
     6301 gacttaacag agacggcctt aaagtgtatc ctaatagtac tttattgaag gatcgagtgt
     6361 aacgaatgga ttattcagaa ctcagcatgc accccgacct ggccaagggc gttcaggacg
     6421 ccggctacgt gacctgcatg ccggtacagg cggcggtatt cccgcacgcc ttcgcgggcg
     6481 aggacatcta cgcccagtct cagacgggca ccggcaagac ggccgcgttc ctcgtctcca
     6541 tcttccagcg catcatgagc gactccgcca gccgccatcg caaggttctc atactcgcgc
     6601 cgacgaggga gctggccgtc cagatagagg aagaggctaa gaccctcggc aagcacctcc
     6661 ctatatcgat cggcagcttc tacggcggtg tcgcctacgg cccccagctc gacatgctcc
     6721 gcgataacgt gcagatgctc atcggcacgc ccggtcgcat catcgacctc gtccagcagg
     6781 gcaagatgct gctccgcgac gtcggcttcc tcgtcgtcga cgaggccgac cgcatgttcg
     6841 acatgggctt catcgacgac ctgcgcaagc tgctccgtta cctaccccag cccaaggaac
     6901 ggcagacgtt cctattctcg gccaccctga acttcaggat caagaacctg gcttgggagt
     6961 acatggacga gcccaaggag atcgtgatcg aggcggagac cgtgacggtc gacctcgtca
     7021 cccaggaact gtaccacgtg ggttccgacg agaaattccg cgtgctgctc ggcatcctcg
     7081 cccgcgacaa gccgtcgagc gccctgatct tctgcaacca gaagttcatg gccgaggaga
     7141 tctccaggcg gctggggatc aacggctacg agtgcgaata catcatgggc gacctgcctc
     7201 aggccaagcg gctccaagtg atcgagcggc tcaaggccgg caagctgtcg atgctcgtgg
     7261 ccaccgacgt ggcggcccgc ggcctcgacg tcgaggcact cgacctggtg gtcaactacg
     7321 acgtaccgat ggacccggag tcatacgtgc accgtatcgg gcgcacggcc agggccggcg
     7381 ccaccggcaa ggccgtgacg ctcgcctgcg agaaattcgt ctacgggctc cctgggatag
     7441 agaagtacat cgaggccaag atacccgtat gccccgtgac cgaggaactg ttcggcgatg
     7501 acaagagcgc cggcatacgt ttcggccact cgcacgaccg cgccaggccg ggcagcgacc
     7561 gaggcgccgg acacggcggc tcgtcgtacc gcggcggcgg ccagggcgcc gggcgcggct
     7621 acggcgatcg cgggccgcgc aggggaccgt ccgaccgggc ccagccgcag cgaggctcgg
     7681 ctccgaggcc gaagcaggct tcccaggcgg gcgggcctca ggccggcgcg ccgcgaccag
     7741 cccatcaggg aggccgcgac ccctacgcca taagttccga ggagcgcatg aggcgctaca
     7801 aggacaagta tgcaggcggc caggccgacc gcgggcagga accggggcgt cccgccgatg
     7861 gacagcagag gcaaagccaa caacggcaaa gccaacaacg gcagggacag tcgaggcgcc
     7921 aaggccagca acccgctcag gggcaggcgc agcgccaaca gccgcgccag gctcaacgcc
     7981 cggcatcgca gggcacgaaa ccccaggcct ccaaaccgca aggaaccggg cctcgggctc
     8041 aaggcagcat gccgcaggct aacaacgccg ggcgtccggc caagcccggc ctgatcgacc
     8101 ggatcaagcg ggcgttcggc ctcggcaagc gcgccgacga ctgagcggcc gagggcgcga
     8161 tgaacgatcg gggcgacttc aggcggccgc gcctggatcc ggaacagctg ttctccccgt
     8221 tcgatcgcgc cctcgggacg ctccacgagc ggctgttcgg ccgctatcgg ctggtcaccg
     8281 tggcggcgtc catactgatc tactcagccg cggtgctatc gaccggggcg tcgcttggcg
     8341 tttcaaccaa ctacttcgta ctgctccccg ttatctgcgc ctcgatgggc tatggtctgt
     8401 tcgccggcgt ggtcgcgggt atcgtcgcct tgcccggcaa ccttatactc tatcgcgcgc
     8461 tcggtcatcc cgagttcgcg ccggcctcga aggcgatggc cgagctttcc ggcatactgg
     8521 tcggcaccgt gctcggctac ctttcggact accatcgcaa gctcgaagtc gagcgctact
     8581 tgcgcaagga aaccgagagt gagctcaggc gggccctccg cgaccgcgag gcgctgttcc
     8641 gcgaggtaca ccaccgggtc aagaacaacc tgaacctgat caagagcatc ataggcctcc
     8701 aggccaggcg ttccgccgac ccggccttca aggacgccgc cgccatgctg accggcagga
     8761 tcatgtcgat ctcattcgtc cacgagcggc tataccgcac ctccgagctg tcggcggtag
     8821 cggtcgacga gtacctcaag gatctggtca acgccgtggt gatggccgcc ggcggctccg
     8881 atacgtgccc gaccgtcgcg ttcgacctgt cgaggcacga cgccagcatg gacgtcgcgg
     8941 taccgctcgg cctgatcgtc aacgagctgg tcacgaattc gatcaagcac ggccagccga
     9001 gacggggccg accgctagcg atggagatac ggctaagcac ggacggcgac gagctatcgt
     9061 tatcggtacg tgacgacgga gtgggcttgc ccggcgtcga cgagggtcgg cggctcgcca
     9121 tcgaggaggc cgtactgcgc ttcccgggta gcctcggcct gaccttgctc gacctgatgg
     9181 cgtcccagct cggcggaaag gccgccttta gccgttccga cggctggacc gaattcaacc
     9241 ttatcttccg cgatgccgcg gcggctgaac gtccctagcc aactggctca ggatagatcc
     9301 tcggcctcgc cggatttcgc ccaccatcgc ccgcctcggc ctctgcctcg gcccatgccc
     9361 cgtccgggac cgcgaacggg accgacggtc tcgacgtcga tcctggccgc ctcgtcccgg
     9421 cggatcagca agtcgtatcc gtggatacgc acctgcatcg gcccgccgaa gccgcctcgc
     9481 ctgacgatga cgccttccgt accctcggta cagcccatat cggcgagcct tcggccgacc
     9541 tcgcggccga cgctgacgga tcggatgatg aaaggttcat gaggccttac aagagaaagc
     9601 tgcatcgaat gcttccttaa ataaagaacg gcggtagagg ccgatattga ttgtcattat
     9661 catcatcgga aggcgaatgg tcaagcgcgc ttctttgacg gaagtcgata acgaggatat
     9721 gcttgctcgg tgcgcaggtt cctcaaggtc gtcatagtcg tatccggcct ggccttattg
     9781 gtcacgttgg ccgcctccat agccctcgcc tcgcggatgc gcgacggcgc gcggtcgccg
     9841 gacgccaggc agggcgggca ggccctcttc catatactcg ccgtggtgcc ggcttcggac
     9901 gccgagcgct actacgcccg cgccgtcgag gccatgacca ggacggctag gaagcgcgga
     9961 gccgtgctgc agacgctcga gtacgaccag gataacgcgc cgagcgcgat agccaggcaa
    10021 ctacgcctgg ctgccgagct cgaaccggat ggcgtggtcg tctccgcgcc gctcgacccg
    10081 ctggtgaccg aggccgtcga caccctctcc aagcggggcg taccggtggt ggccctcgag
    10141 accgacctgc caggctcggc gcgcgcggcc ttcatcggca cgaatcccta ttcggtcggc
    10201 gtgctcgcgg ccgaggccgt ggccgagga
//
LOCUS       DAOOLN010000002         7874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_10838_length_7874_cov_3.263461, whole genome shotgun sequence.
ACCESSION   DAOOLN010000002 DAOOLN010000000
VERSION     DAOOLN010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7874
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10838_length_7874_cov_3.263461"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="PLW80_00055"
     CDS             <1..790
                     /locus_tag="PLW80_00055"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPB64919.1"
                     /translation="DGPDVLVVQEAENARALRDLAEALGGYGYVLSSPDEEATLSCGI
                     LSRVPVTAFRAHRVRQPESSPSSVPRYLLEAELDAGGRRLVVMAAHLKSKLGGAADTE
                     SERRAAAAFISSIVAARLAEDPTLAVVVAGDFNENPDERERVGAAYPTALMPAKAGPG
                     PWLLISGDRAGLEPRDGQPVLFCPWDESGGYSYRYDGADERIDQLLLSPGPLAAGDCP
                     WAYSGFSAEPPEFAVDDSGQPLGWHNGSGYSDHLPIRVSLSPSP"
     gene            841..2520
                     /locus_tag="PLW80_00060"
     CDS             841..2520
                     /locus_tag="PLW80_00060"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPB64920.1"
                     /translation="MKNRPLIAQSDQSLLLDAHDPAFDEARAELSAFATLEKSPEHFH
                     AYRLDALSLWNAASAGLSADAVVATLERWSRYELPASLGQAIRDTMARFGRIVMSEGP
                     DERTLRLACDDRFVRAELAGSKKLGKYLSPDGDGFLVALTERGTVKRELSKLGWPALD
                     TAPLLDGDPFPLALRATRPNGSPFSLRPYQEDAAAAFSGRGAPGTGYGVVVMPCGAGK
                     TVVGMATMAAIGRKTLIVTTNVAAVHQWMDELLDKTDISRDDLGEYTGAAKDVKPVTI
                     ATYQVLTWRPDKESDFPHFDLFRRERWGLIIYDEVHLLPAPVFRVVAEIQAIRRLGLT
                     ATLVREDGLQDDVFSLIGPKRYDVPWKELEAKGFIAEAFCREIRVPMADDVRMRYVAC
                     GTRERHRVAAENPIKSKVVREILDNHPQDLVMVIGQFLDQLRTLAAELNAPLITGATP
                     NPERERIYADFRTGRVRTIVVSKVANFAIDLPDASVAIQVSGTFGSRQEEAQRLGRVL
                     RPKEKNSYFYSIVSRYTIEEEFADNRRRFLTEQGYRYTIEAWEEAEWAPTR"
     gene            2502..4568
                     /locus_tag="PLW80_00065"
     CDS             2502..4568
                     /locus_tag="PLW80_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64921.1"
                     /translation="MGADALNRWRDALLVDRDDTAIAAAKRWLGPVRTPFNKHELIAR
                     LEAFLKKPETASAVVGLLDRTERRILALALYSGTAPTDGLPRLELAKLASDEPAFEAS
                     SLERIRSLHDRLALYSYRDGRGRERVAVAPPLLGRLAAELSPSDALSSAKACESPRAP
                     DPFAAFCALVSACSHAKPAFKGRRELSKRASELLEAAIPGLAQDKERLAALLAGLEAC
                     GAFGVGDDGRPAAYPRRFVELCAEAGTAAPLALAAGCVWRGQALFGVHDDGHLVPATL
                     RASIEALPIGLAYSPADLRRLCAMAIRRRLEALAAAGVPGAEEALDHGLAGAVSATAE
                     ALGRLGVVALGGDGLVRTTSAAAALLDPPAGSGPGNVIVEESHELRILPEADPATRAF
                     IASVARLESAGLVWSAYLDKAAAKTAYAYGFRATGIAAELERLSGLPLPQSVRFSLDA
                     WEDESRSARLRVGIVLALDGHLAGMLEHSPKAEGLVRERLADGVYLLAAGDAAEAERL
                     LKAAGIDVDMRQDPRRDGGEARPLWASSSIDLSPSDATPPLAFRPRNDTLIDEGAPLV
                     ERLLAALDRLELPTEEKARLEAGVRARLVLGEEEFDRARAEEEPDSVGATDYPGKVRL
                     LEKAMKEGSRVELDYIDDTGRRLSAAGVPRDIRRTSSGALVTVSTDNGAQAVIPIRAI
                     TAARRT"
     gene            4591..5364
                     /locus_tag="PLW80_00070"
     CDS             4591..5364
                     /locus_tag="PLW80_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPB64922.1"
                     /translation="MRKTTDYDAGQLKLAVLIDADNTQPSIAEGLLAEVAKYGVASVK
                     RIYGDWTTTKLTQWKAKLLEYAIQPIQQFAYTSGKNATDSAMIIDAMDLLYTEKLDGF
                     CVVSSDSDFTRLAARMREAGKLVLGFGERKTPKPFVSAVDKFIYTDILRGPAEEETAA
                     KDEPKRKAPAPQDLKIDRKLKALLCDAVEDSADETGWAYLGPVGQYVANRQPDFDPRN
                     YGFRKLAELIRSCGWFEVEERSSPTDTGKQIYIRKRQGR"
     gene            complement(5371..7593)
                     /locus_tag="PLW80_00075"
     CDS             complement(5371..7593)
                     /locus_tag="PLW80_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64923.1"
                     /translation="MLGFGADGASLDALTAAGARLWEGCSQYVRLGKAPLRSTIDPGA
                     ALRRLVADFSSAGRYDEFRYLIEYVERSVASGRLVIGSVDDVPPLPGFWPARVGAAKR
                     AIVVNADELASLPQDDAVYRSSFLRAAAALYYADASPAVPGVHSGRNAAAELVAMMSG
                     LYVESIYLLEVLRLAEPEGTAVDYIDLLLGSAVYDNLRGAASALFRVDLDYAYDAVNA
                     ATKPASARGLADYLERAARELVELEAIVSTASLRASPGREDLEPAGRARAALIALPWA
                     AREAAQGSYGSDPSVRRALAGLVEALSSMASAYAPLDPLAMEETRRFLNDLRFRAPDP
                     LAPVVGEPLPDYVEPVGPSGPPAFPGSAEERWYRLCLYDNPYEPVGELPIDFEAAMLG
                     AAYRFSYGSEGGLLAIEHFIYGRLRPSDLLQWAARIRTGRDATGAWSVWESSYGYRMM
                     NEAGYAMRRESRTRNGLSLSFYATDGRRVPDCSNTYIVGIEPRNGGYDLSYRTADGRL
                     WSGYYGYALDSRRIAQGGEETRSHFDELGRPVVTLSLGFHRSVSVKERRGGALVVDMA
                     FFDEAGAPAITFEGYYRQRVRLDEEGRTYELLDVDGEPMTSHLGWAVERIDWDRGLPV
                     RLAFSAPDGGAAISMEGFHEARYELSPDGSIVGLSFYGTEGEPRSSYEGYHRREGEYD
                     DKGALIGVEYFAADGSPSSDRSGASRIRWYLGADGSVESVRVWDAHGALIAGEPSRAL
                     "
     gene            7630..>7874
                     /locus_tag="PLW80_00080"
     CDS             7630..>7874
                     /locus_tag="PLW80_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64924.1"
                     /translation="MPYSANHGPSGLSIVGRDEIDHDRIQETEFHYERSCRVDGSTGE
                     VGQAIGRTAQLTERRTDRMVILPGAVPSAVSSDRATTT"
BASE COUNT         1341 a         2759 c         2642 g         1132 t
ORIGIN      
        1 cgacggcccg gacgtgctcg tcgtgcagga agccgagaac gccagggcgc tccgcgacct
       61 ggccgaggcc ctcggcggtt acggatatgt cctatcgtcg ccggacgagg aggcgacgct
      121 atcctgcggc atactgtcgc gcgtccccgt gactgccttc cgagcccatc gcgtccgcca
      181 gcccgaatca tcgccatcgt cggtgccgcg ctacctgctc gaggccgagc tggacgcggg
      241 cggaaggcgt ctcgtcgtca tggcggccca tttgaagagc aagctcggcg gcgccgcgga
      301 tactgaaagc gagcgtaggg cggccgccgc cttcatatcg tccatcgtcg ccgccaggct
      361 cgcggaggac ccgacgctgg ccgtcgtcgt ggccggcgac ttcaacgaga acccggacga
      421 acgcgaacgc gtcggagccg cctacccgac cgctctgatg ccggccaagg cggggccggg
      481 cccttggctc ttgatatcgg gcgaccgcgc cggactggag cctcgcgacg gccaaccggt
      541 gttgttctgc ccctgggacg agtcgggtgg ctatagctac cgctatgacg gagccgacga
      601 gcggatagac cagctgctcc tgtcgcccgg gccgctcgcc gccggcgact gcccctgggc
      661 ctactcgggc ttcagcgccg agccgcccga attcgccgtg gacgactcgg gccaaccctt
      721 gggttggcat aacggctcgg gctactcgga ccacctaccg atacgggtat cgctgtcccc
      781 gtcgccctga gccgccgcgt ctttgacagc gcggggcggc gcgtggtaca aagaccggat
      841 atgaaaaacc gcccgttgat cgcccagagc gaccaaagcc tgctcctcga cgcgcacgac
      901 cccgccttcg acgaggccag ggcggaacta tcggccttcg ccaccctcga gaagagcccc
      961 gagcatttcc acgcctaccg actagacgcg ctatctctct ggaacgccgc gagcgccggc
     1021 ctatcagccg acgccgtcgt cgcgacgctc gagcgctggt cccgctacga attgccggcc
     1081 tcgctcggcc aggccatacg ggacacgatg gcccgcttcg gccgcatcgt catgtccgag
     1141 ggaccggacg agagaacgct caggctggcc tgcgacgacc gcttcgtccg ggcggagctg
     1201 gccggttcca agaaactcgg gaagtaccta tcgcccgacg gcgacgggtt cctggtcgcg
     1261 ctgaccgagc gcggcaccgt caaacgcgaa ctgagcaagc tcggctggcc ggcgctcgac
     1321 acggccccgc tgttggacgg cgaccccttc ccgctcgccc tcagggcgac gcggcccaat
     1381 ggctcgcctt tctcgctcag gccctaccag gaggacgcgg cggccgcctt ctcggggcgc
     1441 ggcgcgcccg gtaccggcta cggcgtcgtc gtgatgccct gcggcgccgg caagaccgtc
     1501 gtcggtatgg cgacgatggc cgccataggc cgcaagacgc tcatcgtcac cacgaacgtt
     1561 gccgccgtcc atcaatggat ggacgagctt ctcgacaaga cggacatctc gcgggacgac
     1621 ctcggcgagt acacgggcgc ggccaaggat gtgaagccgg tcaccatcgc cacctaccag
     1681 gtgctcacct ggcggcccga caaggaatcg gactttccgc acttcgacct attcaggcgg
     1741 gaacgctggg gcctgatcat ctacgacgag gtgcatctgc taccggctcc ggtcttccgg
     1801 gtcgtcgccg agatccaggc catacggcgc ctcggcctta ccgccacgct cgttcgcgag
     1861 gacggcctcc aggatgacgt cttcagcctg atcgggccca agcgctacga cgtgccctgg
     1921 aaggagctcg aggccaaggg tttcatagcc gaggctttct gccgcgagat acgcgtgccg
     1981 atggccgacg acgtgcggat gcgctacgtc gcctgcggca cgcgggagcg ccaccgggtg
     2041 gccgccgaga acccgatcaa gtccaaggtc gtccgcgaga tcctggacaa ccacccccag
     2101 gatttggtca tggtcatagg ccagttcctc gaccagcttc gcacgctggc cgccgagctg
     2161 aacgcgccgc tgatcaccgg cgccacgccg aaccccgagc gggagcgcat ctacgccgac
     2221 ttcaggaccg gtcgcgtgcg gaccatcgtc gtatccaaag tggcaaactt cgccatcgac
     2281 ctacccgacg cctcggtggc catccaggtc tcgggcacct tcggctcgcg gcaggaggag
     2341 gcgcagcggc tcggcagggt actgcgcccg aaggagaaga actcctactt ctactcgatc
     2401 gtctccaggt acacgatcga ggaggaattc gccgacaacc gccggcgatt cctgacggaa
     2461 cagggctacc gctacacgat cgaggcctgg gaggaagccg aatgggcgcc gacgcgctga
     2521 accgctggcg cgacgcgctg ctggtcgacc gcgacgatac ggctatagcc gcggccaagc
     2581 gctggctcgg cccggtcagg acgccgttca acaagcacga gctgatagcc aggctcgagg
     2641 cgttcctgaa aaagccggag acagcctcgg ccgtcgtcgg gctgctcgac cggacggagc
     2701 gccggatact ggcgctcgcc ctgtactctg gtacggcccc gaccgacggg ctaccgaggc
     2761 tcgagctggc caagctcgcc tccgacgagc cggccttcga ggccagctcg ctcgagagga
     2821 tacgaagcct gcacgaccgg ctcgcgctct acagctaccg ggacggcagg ggacgcgaga
     2881 gggtcgccgt ggcgccgccg ctgctcgggc ggctggcggc agagctgtcg ccgtcggacg
     2941 cgctgtcgtc ggccaaagcc tgcgagtcgc cccgtgcgcc cgatcctttc gctgccttct
     3001 gcgcgctggt atcggcctgc tcccacgcca agccggcctt caagggccgg cgcgagctaa
     3061 gcaagcgcgc gtcggagttg ctcgaggccg cgatcccggg cctggcccag gacaaggagc
     3121 gcctcgcggc cctactcgcc gggctcgagg cctgcggcgc cttcggcgtc ggggacgacg
     3181 ggcgtccggc cgcctacccg cgccgcttcg tggaactgtg cgcggaggcc gggacagccg
     3241 cgccgctcgc gctcgccgcc ggttgcgtct ggcgcgggca ggccctattc ggcgtccacg
     3301 acgacgggca cctcgtaccg gcgacgctac gcgcttcgat cgaggcctta ccgatcggcc
     3361 tggcctattc tccggccgac ttgcgcaggc tctgcgcgat ggccatcagg cggcgcctcg
     3421 aggccctcgc cgcggccggg gtccctggcg ccgaggaggc gctcgaccac ggcctggccg
     3481 gagccgtctc ggccacggcg gaggcgctcg gccggctcgg cgtcgtcgcg ctcggcggag
     3541 acggccttgt caggacaacc tccgcggccg cggccctgct cgacccgccc gccggatcgg
     3601 gcccaggcaa cgtcatcgtc gaggaatccc atgagctccg gatcctgccc gaggccgacc
     3661 cggcgacgcg ggccttcatc gcctcggtcg ctaggctcga gagtgccggt ctcgtctggt
     3721 cggcctacct cgacaaggcc gcggccaaga ccgcttacgc gtatggcttt cgcgccacgg
     3781 ggatagcggc cgagctggaa cgcctctcgg gcctcccgct accgcagagc gtgcgcttct
     3841 ccctggacgc ctgggaggat gagtcgcgtt cggctaggct cagggtcggc atcgtcctcg
     3901 ccctggacgg acacctggcg ggcatgctcg agcattcgcc gaaggccgag ggtctcgtgc
     3961 gggagcgcct ggctgacggc gtctacctgc tagcggccgg cgacgccgca gaggccgagc
     4021 gcctgctcaa ggcggccggc atcgacgtag acatgcggca ggaccccagg cgggacggcg
     4081 gcgaggccag gccgctctgg gcctcctcat cgatcgacct ctcgccttcc gatgctacgc
     4141 cgccccttgc gttcaggccg cggaacgata cactcataga cgagggcgcg ccgctcgtcg
     4201 aaaggttgct cgcggctctc gatcggctcg agctaccgac cgaggagaag gcacggctcg
     4261 aggcgggcgt cagggcgagg ctcgtgctcg gcgaggagga gttcgaccgc gcccgtgccg
     4321 aagaggagcc ggactcggta ggcgccacgg actatccggg caaggtccgg ctactcgaga
     4381 aggctatgaa agaaggctct agggtcgaac tcgactatat cgacgatacg ggccggagac
     4441 tctcggccgc tggcgtaccg cgggacatcc gccgcacgtc atccggagcc ctggtaacag
     4501 tatcgaccga taatggagcc caggccgtca taccgatacg cgccataacg gccgcgcgtc
     4561 ggacgtaaca gaccgaattt ggagtaacga atgagaaaga ctaccgatta cgacgccggg
     4621 cagctgaaac tggccgtgct catcgacgcc gacaacacgc aaccgagcat cgccgagggc
     4681 ctgctcgccg aggtggccaa gtacggcgtg gccagcgtca agcgcatcta cggcgactgg
     4741 acgaccacca agctgaccca atggaaggcc aagctgctcg agtacgccat ccagcctatc
     4801 cagcagttcg cctacacgag cggcaagaac gccacggaca gcgccatgat catcgacgcg
     4861 atggatctgc tgtacaccga gaagctcgac ggcttctgtg tcgtatcgag cgactcggac
     4921 ttcacgcgcc tggccgcgcg tatgcgcgag gcgggcaagc tcgtgctcgg cttcggcgag
     4981 cgcaagacgc ccaagccctt cgtgtcggcc gtggataaat tcatctatac ggacatcctg
     5041 cgcggccccg cggaggagga gacggcggcc aaggacgagc cgaagcgcaa ggcgccggcc
     5101 ccccaggacc tgaagatcga caggaagctc aaggccctgc tatgcgacgc ggtggaggac
     5161 tcggccgacg agacgggctg ggcctatctc gggccggtcg gccagtacgt ggccaaccgg
     5221 caaccggact tcgacccgcg caactacggc ttccgcaagc tggccgagct gatacgctcc
     5281 tgcggctggt tcgaggtcga ggagcgttcc agcccgaccg acaccggcaa gcagatctat
     5341 atccgcaagc gccagggacg ctagcctcgc ttacagcgcc ctcgacggct ctcccgctat
     5401 cagggcgcca tgcgcgtccc agacccgcac cgactcgacg gagccgtccg cgccgaggta
     5461 ccatcgtatc ctcgaggcgc cgctcctgtc ggacgagggc gagccgtcgg ccgcgaagta
     5521 ctcgacgccg atcagggcgc ccttatcgtc gtactcgcct tcacggcggt gatagccctc
     5581 gtaggacgaa cgtggctcgc cctcggtgcc atagaacgac aggccgacga tggagccatc
     5641 gggcgacaac tcgtagcgag cctcgtggaa gccctccatc gagatggccg cgcctccgtc
     5701 cggagcgctg aaggccagac ggaccggtag gccgcgatcc cagtcgatcc gctcgacggc
     5761 ccagccaagg tggctcgtca tcggctcgcc gtcgacgtcc aacagctcgt aggttcggcc
     5821 ttcctcatcg agcctgacgc gctgtcggta gtagccctcg aaggtgatag ccggagcgcc
     5881 cgcctcgtca aagaacgcca tgtcgacgac cagcgccccg ccccgccgct ccttaaccga
     5941 gacggacctg tggaaaccga gcgacagggt gacaaccggt cggccgagct cgtcgaagtg
     6001 cgaccgggtc tcctcgcctc cctgggcgat gcgccgcgaa tcgagcgcgt agccgtaata
     6061 gccgctccag agccggccat cggcggtccg atagctcaga tcgtagcctc cgttccgagg
     6121 ctcgatgccg acgatatacg tattcgagca gtccggcacg cgacggccgt ccgtcgcgta
     6181 gaaactcagg ctcaggccgt tccgcgtccg gctctcgcgc cgcatcgcgt agccggcctc
     6241 gttcatcatc cgataaccgt acgagctctc ccagaccgac caggcgcccg tcgcgtcgcg
     6301 tccggttcgg atcctggcgg cccactggag caggtcggac ggcctcaggc ggccgtagat
     6361 gaaatgctcg atcgccagga gaccgccttc cgaaccatac gaaaagcggt acgcggcacc
     6421 gagcatggcc gcctcgaagt cgataggcag ctcccccacc ggttcgtacg ggttgtcata
     6481 gaggcagagc ctataccagc gttcttctgc ggagccgggg aaagccggag gcccggacgg
     6541 tccgacgggc tcgacgtagt cgggcaaagg ctcgcccacg acaggagcga gcgggtccgg
     6601 agccctgaac cgcagatcgt tcaggaagcg cctcgtctcc tccatcgcga gcggatcgag
     6661 cggcgcgtag gccgaggcca tcgacgacag cgcctcgacg aggcctgcga gcgcccgccg
     6721 gacggacggg tcggaaccgt agctaccctg ggccgcctcg cgcgcggccc agggcagcgc
     6781 tatgagcgcg gcgcgggccc ggccggccgg ctcgaggtcc tcacgccccg gcgaggcccg
     6841 tagcgacgcg gtcgagacta tcgcctcgag ctcgacgagc tcgcgcgcgg cgcgctccag
     6901 gtagtcggcg agcccccggg ccgacgcagg cttggtggcg gcgttgacgg cgtcgtaggc
     6961 atagtcgagg tccactctga acagggcgga cgccgcgccg cgcaggttat cgtagacggc
     7021 gctgccgagc agcaggtcaa tataatccac ggccgtgcct tccggctcgg ccaggcgcaa
     7081 cacctcgagc aggtagatcg attcgacgta gaggccgctc atcatggcga ccagctcggc
     7141 ggcagcgttg cgccccgaat ggacgccagg caccgccggc gaggcgtcgg catagtagag
     7201 ggccgcggcc gcccgcagga acgaggagcg gtagacggcg tcgtcctggg gcaggctagc
     7261 cagctcgtcg gcgttgacga cgatggcccg cttcgcggcg ccgacgcggg ccggccagaa
     7321 gccgggcagg ggcggcacgt cgtcgaccga gcctatgacg aggcggcccg acgcgacgga
     7381 acgctccacg tactcgatca ggtagcggaa ctcgtcgtag cgccccgcgg acgagaaatc
     7441 ggccaccaag cgcctgagcg cggcacccgg atcgatcgtt gaccggagag gcgccttgcc
     7501 caggcgtacg tactgcgagc agccctccca gagtctggca ccggcggcgg tcagagcgtc
     7561 gaggctcgct ccatcggccc cgaaccccag cattgcggtt acggccgcga acaaaatgag
     7621 cgcaatgcaa tgccgtactc tgcgaatcat gggccctccg ggctatcgat agtaggccgg
     7681 gacgagatcg accacgaccg aatccaggaa accgagttcc actacgagcg atcctgccgc
     7741 gtcgatggga gcaccggcga ggtcgggcag gcgataggcc gtaccgctca gctcaccgag
     7801 cgccgtaccg ataggatggt gatcctgccg ggcgcggtac cttccgccgt ctcgagcgac
     7861 agggcgacga cgac
//
LOCUS       DAOOLN010000003         7713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_11176_length_7713_cov_3.556934, whole genome shotgun sequence.
ACCESSION   DAOOLN010000003 DAOOLN010000000
VERSION     DAOOLN010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7713
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11176_length_7713_cov_3.556934"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PLW80_00085"
     CDS             <1..397
                     /locus_tag="PLW80_00085"
                     /inference="COORDINATES: protein motif:HMM:NF016230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPB64925.1"
                     /translation="AGLENATRIIGVRVQDEAVTRPLPEPRYEYLPDQAIVCRCERVT
                     VGEMVAFIKANEVRDASQLKSIRVGMGACGSKTCSALLPQVFRKAGVDPREVTDGTLR
                     PLYLEAPLGELAGRVAGPVGADERNGGRA"
     gene            394..1560
                     /locus_tag="PLW80_00090"
     CDS             394..1560
                     /locus_tag="PLW80_00090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB64926.1"
                     /translation="MRTCDVLIIGAGSVGVPLALYCAERGLSTIVLEKNASWGRGQNR
                     AAIGGLRATHSDPAKIRLCQESIRIVSGLEAERGIDVEWRAGGYLFVAYDEQRERAFR
                     DLLVTQKAAGLDIDWIGPERVAELCPGIRRDGLLGGTFSPGDGYASPLMTNTAFHRLA
                     RAAGAEFRFGEGVVSMATTPDRVSGARRIASVRTDKDEYSAAVVVDAAGADAADIAGL
                     VDLEIPVHPDCHEAGVTEPCERFLEPMLVDIRPDDESGNYYFYQTVTGQIVFCITPKP
                     QIWGKDTDSTSSFLPLVSRRMIELYPRLRNLKVRRTWRGMYPMTPDGLPIVGYPRECP
                     NLLLAVGMCGQGFMLGPGLGRLLADAIADGPEAAADVFEELSLYRKFEGMELLK"
     gene            complement(1692..2177)
                     /locus_tag="PLW80_00095"
     CDS             complement(1692..2177)
                     /locus_tag="PLW80_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64927.1"
                     /translation="MGCLEYFLDVIDAEFFGKSWNGPSLMATLDRLSAAEAADDATWE
                     GYSAWSIALHCAKCKRVVAVDLGVRAPDWDYGEETWFPAPADPSPSAWERDRALLRVM
                     HDLAMGALRALPEERLAEAMPSWKRPWGDVIAWLATHDAFHGAQIRSMGLTAFKEKRH
                     E"
     gene            2356..6441
                     /gene="pgk"
                     /locus_tag="PLW80_00100"
     CDS             2356..6441
                     /gene="pgk"
                     /locus_tag="PLW80_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPB64928.1"
                     /translation="MIIDEQAKRPARLDTWEAVLEQLGRVRDAADEAEKIELLVELIV
                     NLRLLGQDQAIPDRRTRSGDMESIYADLERATRAMLDSAVSPSSRFIYYAREVVKKLP
                     IKQDAEDMIEQIRMAAEAFEEERDEDDPMRGFAGDLRKEVHRRLSPRIIHAYLEPYLA
                     LVERGDPEPMIRAGYVALPGRVDLADERGSFADLARDLKERLDYLWDYGVGDMAAVKR
                     SLEQSMPLFERCFLRAEVSGFLEMLTPERLAKRHVAADMLKRLASFKRSIKESFTESR
                     ISLYDMLLVDLSLGRLIFLLANDLTNNRFLEVTPRNGKEALGVLAELLNISTVKGLAI
                     GDADRNLLALEELRAQSTYDYVRAKRCLGSICDELQGYLQRDIIDRMSGPLNRALEAY
                     GVPTSRLSQIKTRFFNNFIRRTQVHVLSEFVEKVRSAIDAELERQSVSRQLYADYRLA
                     EGGAAPAREACVAATWRETHDSIRPTLGGKGNGLIDMARLGLAVPPAFILGYPIFAAC
                     DADGELDAGLGAIVDEQLAELERRCGRRLGDPERPLVVSVRSGAPVSMPGVMVTVLNV
                     GLVPEVRRAICRRSDCDFAEALYRRFLDNAAGALAAMPPRTDSDQRERAPAELKSAPT
                     DELEAYLRASLGDGFLSDPREQLMQSIRLVYASRKSSAVDAFSRTLATTIRADTAVTV
                     QRMVFGNLNDASMSGVVITRDPITGADRLFGDFKRRAQGEEVVMGSVDTESIEHVDEA
                     VAAELEACKRRLVDYYRQDLDLEFTVEDGELYLLQTRAAKLGAFAQLVADKEFLERGL
                     IRLEEFHSRLERLEMANASIALPRADFRFRRWNPPLAVGVPINGGVVSGTLVLSDDCL
                     KEAEARRESVVYLAHTTKPTDFTVMNGANAIVTVYPGRTSHAAITAMSINRTCIVGVE
                     SAELDLAARLVRFRLADGSGHVTVAEGERVTADGNTGAIYRGVAPISEFFLPVSVITE
                     AIRREEDPVEAARAVGALIDEKLSGLSRETSMRRSGLDSPAARGMGRVIVRVDANVAL
                     ADGAVADRRSILRFADTLRTLLDRGATPIVCSHLGDPGAITGSVRSREEQYRDYSLRP
                     IADVLSGILGERFTFHETSVATSGLLVTASDMLEGKVNLLENLRFATGEKDNDDAFAM
                     AIAELSDGWYVNDAFNVCHRRHASIVGVPRFCRNRLAGPQVVAELSVIGALLDEPERP
                     FVAVFRGTSQATDLGAQLGVMSAIINRVDAMLVTGPGVEAIRPRLEALGHQAPGRLVV
                     LPSEAELTEALDGAGTVLWSGASGLSDDGWSEPDASGDPVAAALGRAAARNASLVVCS
                     ATDRYGRGAPGSRLHLSSGPAAFLEFLERLSLPGVTALDPD"
     gene            complement(6438..>7713)
                     /locus_tag="PLW80_00105"
     CDS             complement(6438..>7713)
                     /locus_tag="PLW80_00105"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB64929.1"
                     /translation="ERLEGFYILVSTARRIVDEYIEAFKSFSPNARRFLTGSFLLSVG
                     ANQISLLFSLYLQRLAYTEAGIGAVLSTRAVGSTIIALPASILAARFDPRRLLPVAAA
                     MTAAAYVAQSLFVSGAAISSAVFMAGALSTVFQVSAGPFFMRNSGEKERMHLFSLNGA
                     LSRGTGLIGSPLGGGLKDGLAGLGVDELTAYRVGLLVGAGFVAAAVWPFSRIAPSREP
                     RTMRAPGLRRLDGIDVPLWLKLIFPSFLVGMGAGLTIPYLNLYFKNAFGMSDAAIGAA
                     VAAGQVATFLGIVAGPIIAKRIGKPRAVFWTQVLSVPLILVLGWVRALPLALLAYLAR
                     QVLMNMSSPIQDNFALELVPPERQSLLNAVKMLSWTGSWTVAARVSGELIYRGGFKTS
                     FALTAGLYLASTICYRIFFISGKRVRDGGRGA"
BASE COUNT         1260 a         2755 c         2572 g         1126 t
ORIGIN      
        1 ggccggcctc gagaacgcca ccaggatcat aggcgtgcgc gtacaggacg aggccgtcac
       61 cagaccgctc cccgagccgc gctacgagta cctgcccgac caggctatcg tctgccgctg
      121 cgagcgcgtg acggtcggag agatggtcgc gttcatcaag gccaacgagg tccgcgacgc
      181 gagccagctc aagagcattc gcgtgggcat gggcgcctgc ggctccaaga cctgctcggc
      241 gctcctgccg caggtcttcc gcaaggcggg cgtcgacccg cgcgaggtga ccgacggcac
      301 gctcaggccg ctgtacctgg aggcgccgct cggcgagctg gccggccgcg tcgccggccc
      361 cgtgggagcc gacgaacgaa acggagggcg ggcatgagaa cctgcgatgt actgatcata
      421 ggcgccgggt cggtcggcgt cccgctcgcc ctgtactgcg ccgagcgcgg cctatcgacg
      481 atagtcctgg agaagaatgc ctcttggggc cgcggccaga accgcgcggc catcggcggc
      541 ctgcgcgcca cccactccga cccggccaag atcaggctat gccaggaatc gatccgcatc
      601 gtctcggggc tcgaggcgga gcgcggcata gacgtcgagt ggcgcgctgg cggctaccta
      661 ttcgtcgcct acgacgaaca gcgcgagcgc gccttccggg acctgctcgt cacgcagaag
      721 gccgccggcc tcgacatcga ctggattggg cctgagcgcg tcgccgagct ctgcccgggc
      781 atccggcgcg acggcctcct cggcggcacc ttcagcccgg gcgacggcta cgcctcgccc
      841 ctgatgacga ataccgcctt ccataggctg gccagggcgg ccggcgccga gttccgtttc
      901 ggtgagggcg tcgtttcgat ggcgaccacg ccggaccgcg tctccggagc ccgccggata
      961 gcctcggtgc gcaccgacaa ggacgagtac tcggccgccg tcgtcgtcga cgccgcgggc
     1021 gccgacgccg ccgacatagc cggcctcgtc gacctcgaga taccggtgca ccccgactgc
     1081 cacgaggccg gcgtcaccga gccctgcgag cgcttcctcg agccgatgct cgtcgacatc
     1141 cgtcccgacg acgaatcggg caactactat ttctatcaga ccgtcaccgg ccagatcgtc
     1201 ttctgcatca cccccaagcc ccagatctgg ggcaaggaca ccgactcgac ctcgagcttc
     1261 ctgccgctcg tctcgcgccg catgatcgaa ctgtacccgc gcctacgcaa cctcaaggtc
     1321 aggcgcacct ggcgcggcat gtacccgatg acgccggacg gcctgcccat cgtcggctat
     1381 ccgcgcgagt gcccgaacct gctcctggcc gtgggtatgt gcggccaggg cttcatgctc
     1441 ggcccgggcc tgggcaggtt gctcgccgac gcgatagccg acggccccga ggcggccgcg
     1501 gacgtcttcg aggagctgtc gctgtacagg aagttcgagg ggatggagct gctaaaataa
     1561 gcgctggcgg ccgggcgctg tcagaccggc cgtcagccta gccgtcagac cggccgtcgg
     1621 cctagccgtc agcctagccg tcagaccggc cgtcagccta gccgtcggcc cgaccgtcat
     1681 ccgcccggcc gctactcgtg ccgcttctcc ttgaacgcgg tgaggcccat cgacctgatc
     1741 tgggcgccgt ggaaggcgtc gtgcgtggcg agccaggcta tcacgtcgcc ccatggccgc
     1801 ttccagctcg gcatggcctc ggccagacgc tcctcgggta gggcgcggag cgcgcccatg
     1861 gccaggtcat gcatcacgcg gagtagcgcg cggtcccgct cccaagccga cgggctcggg
     1921 tcggccggcg ccgggaacca agtctcctcg ccgtagtccc agtcgggtgc cctgactccg
     1981 aggtcgacgg cgacgacgcg cttgcacttg gcgcagtgca gggcgatact ccaggcgctg
     2041 tagccctccc aggttgcgtc gtccgctgcc tcggcggccg agaggcggtc gagcgtggcc
     2101 atcagcgacg gcccgttcca cgacttgccg aagaactccg catcgatcac gtccaggaaa
     2161 tattccaggc atcccatggc tctaccccct ccgatcggcg gtccatcggc gccagccggt
     2221 ggcgctccgc cagaggcaag tatcgccgag gccagccgcg ctgtaaaggg actatcaccc
     2281 gaggcaagcc ggagcgaggg tcgagccgtt gaccaggcgc ttcctcctaa gcgacatcgg
     2341 gtttagacta gcgctatgat catcgacgaa caagcgaaac ggcccgccag gctcgacacc
     2401 tgggaagccg tgctcgagca gctcgggcgc gtccgcgacg ccgccgacga ggcggaaaag
     2461 atagagctcc tcgtcgagct catcgtcaac ctgcgcctgc tcggacagga ccaggccata
     2521 cccgaccgcc gcacccggtc gggcgacatg gagagcatct acgccgacct tgagcgcgcc
     2581 acccgggcca tgctcgactc ggccgtctcg ccgtcgtcgc gcttcatcta ctacgcccgg
     2641 gaggtggtca agaagctacc tatcaagcag gacgccgagg acatgatcga gcagatacgt
     2701 atggcggccg aggccttcga ggaagagcgc gatgaggacg acccgatgcg cggcttcgcc
     2761 ggggacctgc gcaaggaggt gcatcgcagg ctgtcgccgc gcatcatcca cgcctacctg
     2821 gagccgtacc tggccctcgt cgagcgcggc gaccccgagc cgatgatcag ggccggctac
     2881 gtagccctgc cgggccgcgt cgacctcgcc gacgagcggg gttccttcgc cgacctggcc
     2941 agggacctca aggagcgact cgactaccta tgggactacg gcgtcgggga catggcggcc
     3001 gtcaagcgct cgctcgagca gagcatgccc cttttcgagc gctgcttcct gcgggccgag
     3061 gtgtcgggct tcctcgagat gctgacgccc gagcggctgg ccaagcgcca cgtggcggcg
     3121 gacatgctca agcgcctcgc cagcttcaag cggtccatca aggagtcgtt caccgagtcg
     3181 aggatatcgc tgtacgacat gctgctcgtc gacctgtcgc tcggccgcct catcttcctc
     3241 ctggccaacg acctgacgaa caaccgcttc ctcgaggtga cgccgaggaa cggcaaggag
     3301 gcgctcgggg tgctcgccga gctactcaac atatcgaccg tcaaaggcct ggccatcggc
     3361 gacgccgacc gcaacctgct ggcgctcgag gagctgcgcg cgcaatcgac ctacgactac
     3421 gtgcgagcca agcgctgcct cggctcgatc tgcgacgagc tacagggcta cctgcagcgg
     3481 gatatcatag accgcatgag cggcccgctc aaccgcgcgc tcgaggccta cggcgtaccg
     3541 acctcgcgcc tgtcccagat caagacgcgc ttcttcaata acttcatacg gcgcacccag
     3601 gtccacgtgc tgtcggaatt cgtggagaag gtgcgctcgg ccatcgacgc cgagctggag
     3661 cggcagagcg tcagcaggca gctctacgcc gactatcgcc tggccgaagg cggagcggcc
     3721 ccggcccgcg aggcctgcgt ggccgcgacc tggagggaga cgcacgatag catacgcccg
     3781 acgctcggcg gcaagggcaa cggcctgatc gacatggcca ggctcggctt ggcggtgccg
     3841 ccggccttca tactcggcta ccctatcttc gcggcctgcg acgccgatgg cgagctcgac
     3901 gccgggctcg gggccatcgt cgatgagcag ctggccgagc tggagcggcg ttgcggccgg
     3961 cgcctgggcg accccgagcg gcctctcgtc gtatcggtac gctccggcgc gcccgtgtcg
     4021 atgcccggcg tcatggtaac ggtgctcaac gtcggcttag tcccggaggt caggcgcgcg
     4081 atctgccgtc gctcggactg cgacttcgcc gaggccctat accgacgctt cctggataac
     4141 gccgccgggg cgctcgccgc gatgccaccc cggaccgact cagaccaacg ggagcgggct
     4201 ccggccgagc ttaagtcggc gcccaccgac gagctcgaag cctacctgcg cgcctcgctc
     4261 ggcgacggct tcctgtccga tccgcgcgag cagctgatgc agtcgatccg cctcgtctac
     4321 gcctcgcgca agtcgagcgc cgtggacgcg ttctcgagga cgctcgcgac gacgatacgg
     4381 gccgacacgg ccgtgaccgt ccagcgcatg gtcttcggta atctgaacga cgcctcgatg
     4441 agcggggtcg tcatcacgcg cgacccgatc acgggcgccg accggctgtt cggcgacttc
     4501 aagcggcggg cccagggcga ggaggtcgtc atgggctccg tcgacaccga gtcgatagag
     4561 cacgtagacg aggccgtggc ggcggagctc gaggcctgca aacgccgcct cgtcgactac
     4621 tacaggcagg acctcgacct ggagttcacc gtcgaggacg gcgaactata cctgctccag
     4681 accagggcgg ccaagctcgg ggccttcgcc cagctggtcg cggacaagga gttcctcgag
     4741 cgcggcctta tacgcctcga ggaattccac tccaggctcg agcgcctcga gatggccaac
     4801 gcgtcgatcg ccctgccccg cgccgatttc cgcttcaggc gatggaaccc gcccctggcc
     4861 gtcggcgtac cgatcaacgg cggggtcgtg tcggggaccc tggtcctgag cgacgattgc
     4921 cttaaggagg ccgaggccag gcgcgagagc gtcgtgtacc tggcccatac gacgaaaccc
     4981 accgacttca cggtcatgaa cggcgccaac gccatcgtta ccgtctaccc gggccgcacc
     5041 tcgcacgcgg cgatcaccgc catgtcgatc aacaggacct gcatcgtcgg cgtcgagagc
     5101 gccgagctcg acctcgccgc ccgcctcgta cgcttccgcc tggcggacgg ctccggccac
     5161 gtcacggtgg ccgagggcga gcgggtaacg gccgacggca acaccggcgc catctaccgc
     5221 ggcgtggctc cgatatcgga gttcttcctg cccgtatccg tcataaccga ggcgatacga
     5281 cgcgaagaag acccggtcga ggcggcgcgc gcggtcggcg ccctgatcga cgagaagctg
     5341 tccggcctga gccgcgagac gagcatgcgg cgctccggcc tcgactcgcc ggccgcccgg
     5401 ggcatgggcc gcgtcatcgt gcgagtcgac gcgaacgtgg ccctggccga cggcgcggta
     5461 gccgaccgcc gtagcatcct gcgcttcgcc gacaccctgc ggaccctgct cgaccgcggc
     5521 gccacaccga tcgtctgctc gcacctggga gaccccgggg cgataaccgg ctcggtacgc
     5581 tcccgggagg agcagtaccg cgactatagc ctgcggccga tagccgacgt cctctcgggc
     5641 atcctcggcg agcgcttcac gttccacgag acgagcgtgg ccacctccgg cctcctggtc
     5701 accgcttccg acatgctgga gggcaaggtc aacctgctcg agaacctgcg tttcgccacg
     5761 ggcgagaagg acaacgacga cgccttcgcc atggccatcg ccgagctgtc cgacggctgg
     5821 tacgtgaacg acgccttcaa tgtctgccac cgccgccacg cgagcatcgt cggcgtgccg
     5881 cgcttctgcc ggaaccggct cgccggtccc caggtcgtcg ccgagctgtc ggtcatcggc
     5941 gccctgctcg acgagcccga gcggcccttc gtcgcggtgt tcagggggac gagccaggcc
     6001 accgacctcg gcgcccagct cggcgtgatg tcggccatca tcaaccgggt cgacgcgatg
     6061 ctcgtgaccg gcccgggcgt cgaggctata cggccgcggc tcgaggccct cgggcaccag
     6121 gcgccgggcc gcctcgtcgt cctgccgtcg gaggcagagc tgacggaggc gctggacggg
     6181 gccggcaccg tgctgtggtc gggcgcgagc ggcctgtcgg acgacggctg gtccgagccc
     6241 gacgcctcgg gagacccggt cgcggccgcc ctcgggcgcg cggcggccag gaacgcgtct
     6301 ttggtcgtct gcagcgccac cgaccgttac ggccgagggg cgccaggctc gaggctacac
     6361 ctgtcgagcg ggccggcggc cttcctcgag ttcctcgagc ggctgtccct gccgggcgtc
     6421 accgccctcg atcccgacta ggcgccgcgt ccgccgtccc ggacgcgctt gcccgagatg
     6481 aagaagatcc ggtagcagat cgtcgaggcg aggtacagcc cggcggtcag cgcgaaggag
     6541 gtcttgaagc cgccgcggta gatcagctcg cctgagacgc gcgcggccac cgtccagctg
     6601 cccgtccagg acagcatctt gaccgcgttg aggaggctct gccgctccgg cggcaccagc
     6661 tcgagcgcga agttatcctg gatgggcgac gacatgttca tgagcacctg cctggccagg
     6721 taggccagca gcgcgagcgg caaggcgcgc acccagccga gcaccaggat cagcggcacc
     6781 gacagcacct gcgtccagaa caccgcgcgc ggcttgccta tccgcttggc gatgataggg
     6841 ccggcgacga tgcccaggaa ggtggccacc tgtccggccg ccacggcggc gcctatcgcc
     6901 gcgtcgctca tgccgaacgc gttcttgaag tacaggttga ggtacggtat ggtcagtccc
     6961 gcgcccatgc cgacgaggaa gctcgggaag atcagcttga gccataacgg gacgtcgatt
     7021 ccgtcgaggc gccgcaggcc gggcgcgcgc atcgtccgcg gttcgcgcga cggagcgata
     7081 cgagagaacg gccacaccgc cgccgccacg aagcccgccc cgacgagcag cccgacccgg
     7141 tacgccgtca gctcgtcgac accgagcccc gcgagtccgt ccttgaggcc gccgccgagg
     7201 ggcgagccta tcagccccgt gcccctcgac agcgcgccgt tcaggctgaa caggtgcatc
     7261 cgctccttct cgcccgagtt gcgcatgaag aacggcccgg cgctcacctg gaacaccgtc
     7321 gacagcgcgc cggccatgaa cacggccgac gatatggccg ccccggatac gaacagcgac
     7381 tgggcgacat aggcggccgc cgtcatcgcc gcggcgaccg gtagaagccg gcgcgggtcg
     7441 aagcgcgccg ccaggataga cgcgggcagc gctatgatcg tcgagccgac cgccctcgtc
     7501 gacagcaccg cccctatccc ggcctccgtg taggccaggc gctggaggta caggctgaag
     7561 agcaggctga tctggttggc cccgacgctc aggaggaagc tccccgtcag gaagcgcctc
     7621 gcgttggggc tgaacgactt gaaggcttct atatactcgt cgacgatacg cctggcggtg
     7681 ctcacgagta tatagaagcc ttcaagtcgt tca
//
LOCUS       DAOOLN010000004         7512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_11596_length_7512_cov_2.969022, whole genome shotgun sequence.
ACCESSION   DAOOLN010000004 DAOOLN010000000
VERSION     DAOOLN010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7512
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11596_length_7512_cov_2.969022"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1118
                     /locus_tag="PLW80_00110"
     CDS             141..1118
                     /locus_tag="PLW80_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64930.1"
                     /translation="MFHPEEVIFAPTGRCNLRCAHCRVRSDGPELAIEAAVSFLESCS
                     AAGIERVGFSGGEPFLRLDLIVAVTAAAVDQGLYFDRLMTNGDWWTTGASLRAALGAV
                     AEAGFDGVIGLSHDAYHGQAPERVAAFMAAAYEAFGRRDAVELLSVRSPDDGAFFRGL
                     ETVATALGGRLEYELSEPARIVDEAYLARSEADPDDGSALVAQVIRSPRSRSAEEGAW
                     DAERWFVDDFCEGPGNVLYVHPDGAVAVCCGFANENPQLIVGSVNDSVEAIVAEAAAR
                     PLVRACYEAGLATTRRRLEAAGVRFPGKTGDACFFCDYLCKRGLVGSRG"
     gene            complement(1125..1844)
                     /locus_tag="PLW80_00115"
     CDS             complement(1125..1844)
                     /locus_tag="PLW80_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HPB64931.1"
                     /translation="MSNDKLRSTGFLFAAFFLGGFMINFVSDIGKHGFFWSTTNPYQL
                     AQLAICAAFILSALVRPLRILQPIVFLVFSPVSIIQSTRNIYGLGFFIIGILLLERTG
                     FYARHRLPKLILSISYLLAIEIVGVVSSGKSPMEASAPTFFIVAFGVFLWFLYKDRLV
                     IILKEPKPKLSLAEKGLSTAERAFIGQTLSGKSQKEISIDFELSESTVRNTLARAYKK
                     LDIEDRVGLAVLGERFEIDSD"
     gene            complement(1831..2304)
                     /locus_tag="PLW80_00120"
     CDS             complement(1831..2304)
                     /locus_tag="PLW80_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64932.1"
                     /translation="MNDSETRAFKNNKVAKLVGLLPFAAGCDDAMRSSLSHLATFVIA
                     NRGRARRFFDHAPADDANPMARLRTIADFKGGDSAALEQGLARLALCMTSGYRRDAEI
                     DRLTGEYNPVNGDTVEPERSAVSSCSEKNGSAVIDSILTADEAIYTFWETPDEQR"
     gene            2502..4058
                     /locus_tag="PLW80_00125"
     CDS             2502..4058
                     /locus_tag="PLW80_00125"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPB64933.1"
                     /translation="MKPAGGQPYRATFLFVAIPIAAAIALAWIVIDKASVRTHLEMAE
                     RNRALQVELARNAMEEGFERLLQENRVLAAYSFPEYARGLRGRASMEALLEAEGDAYP
                     ESLSYCYLTGPGRLELAWARPGAEETMEALVFASVAQWDGFLGMSDPVVLSSRSNRPE
                     PYFMALFPVRVGGDMVGILATAIGLGRSIDTYLSPLAEGEGRRSFLMFGAGRVLWASD
                     DRNPSLFELDQGSLMTSRSFALGDGEFTIIADESRASLLSELEAIDRPRFLVGAMGLL
                     TMVAALFFANRLYLERRGRQALASEERRLSARVAAGERELRALLDDRELLLRELHHRV
                     KNNFQYLDSLIELQKGGASADAAAALSNIQARIAALAAAYLINADRPESLRVDARDYL
                     TALAGKVVDGAGVELVIEAEDIPVSLDSAVPLGLLFHELLTNAVRHGYAGKRGYAGGH
                     GDRVAVRFYRDGGTAVLEVRDFGTGLEAGTADGLGLTIVRALVAQLNGGFSLENREPG
                     VIAEARFPLA"
     gene            complement(4084..4854)
                     /locus_tag="PLW80_00130"
     CDS             complement(4084..4854)
                     /locus_tag="PLW80_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64934.1"
                     /translation="MRLGGRNGAMATRRAASIALAVAVIVAAWSAAAAALGKPFLPSP
                     ATTFAAFGRLAATGSLGRHAAASASRVLWALAASFFPAAALGLAAGRSRRVDAVVAPV
                     VYVLHPLPKVAFLPVIMLVFGLGEASKVILVALIVFSQILVAARDAARRIPRPLVDSV
                     RSLGASRLDLAFRVVVPSTLPDLLTSLRVSLGTAVAVLFLAETFATETGLGYLIVDAW
                     ARVAYAEMYAAIVALSLLGLGFFMAVDLAERLLCPWRS"
     gene            complement(4851..5717)
                     /locus_tag="PLW80_00135"
     CDS             complement(4851..5717)
                     /locus_tag="PLW80_00135"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64935.1"
                     /translation="MVELRELTIDYPGGSSPGWTGRLGRPGPRVPAVAGVDAVFGSGA
                     ISAIVGPSGCGKSSLVGAMAGLLAPSAGEALIDGEPVRGVRPRTAVIFQDYGLLPWLT
                     VEGNAELPLAVRGIGRSERRRRVGPILHELGLSSFGGFYPSRLSGGMKQRVAIARALA
                     SEPDLLLMDEPFSSLDALTREAAQEALLEVRRSRPMTIIVVTHAIEEAAYLAEAVFVM
                     GGRNPGTIRARFDIPENAARVRFAPGAAGDFRSASRYLELCGAIRSALRGSGPTDDSA
                     SPGMAPIPEARR"
     gene            complement(5711..6661)
                     /locus_tag="PLW80_00140"
     CDS             complement(5711..6661)
                     /locus_tag="PLW80_00140"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB64936.1"
                     /translation="MHVHNPILKASLALAVATLFAVAASSCSPKRGAEPLRLGIMPDA
                     DSLPFLVAEAEGLFEKRGVEVELVSFANAQERDAAIQAGRLDGAVSDVLAAAFLAAGG
                     FDTRITSATDGRYGIAAAPGSGLGSAAELAGKRVGLSTNTIIQYSVDAIVGASGLAPD
                     AYTAVAIPKMPVRMEMLLAGQVDAAGLPEPFLTTAIERGATLVGTTEQFHIDAAVVVF
                     SEAVLNARLKDVRRLYEAYADASGRVNAAPESYRAFLVEKARFPEDAAGAYRFVTYRK
                     PTMPPLGQVEAAVSWLRERNLLSKPVDPSALLDDRAVAAW"
     gene            complement(6767..>7512)
                     /locus_tag="PLW80_00145"
     CDS             complement(6767..>7512)
                     /locus_tag="PLW80_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64937.1"
                     /translation="SNVTVVYDDDEPADVTRAEALKAILEDDQGITGLANIYGAMPTW
                     SVTLLPEDLISATYSTEARIYGDPIIVTHGSSFTTASTNDGRVRNIAASSKGTIAMGT
                     GGAYFIYRVDQNWSAWALTGTQPSDIDSGNQMGLNAIRSSKTVASSASEDIWYTPLYY
                     GVLYNSYRESSVAVNIFSADTGRRGIYISSGANPTGGYIYAGDAYSGTYFPVVRQGEY
                     CYYGYYEIPDAPATGQVFLINLVSRMASF"
BASE COUNT         1253 a         2417 c         2591 g         1251 t
ORIGIN      
        1 ctcgcgggta tcggcctgcc agcgaccttc ggggcgctgg cggctatcct cggcgcgctg
       61 gccctgtcgc ccgccgcctc gcccgcgagg cgcgcccggg cacttgagca tgacgccgag
      121 gacgagtagg ctatagtcct atgttccacc cggaagaagt gatcttcgcg cctacgggcc
      181 gatgcaacct gcgttgcgcc cattgccgcg tgcggagtga cggcccggag ctggcgatcg
      241 aagccgccgt ctctttcctc gaatcatgct cggcggccgg catcgaacgc gtcggcttct
      301 caggcggcga gcccttcctg aggctcgact taatcgtcgc ggtgaccgcc gccgcggtgg
      361 accagggcct gtacttcgac cggctgatga ccaatggcga ctggtggacg accggggcgt
      421 cgctacgcgc ggcgctcggc gcggtcgccg aggccggctt cgacggcgtc atcggcctga
      481 gccacgacgc gtaccacggc caggcgcccg agcgggtcgc ggccttcatg gcagcggcct
      541 acgaggcctt cggccgccgg gacgcggtgg agctcctgtc ggtgcgctca cccgacgacg
      601 gggcgttctt ccgtggctta gagacggtcg ctacggccct gggcggccga ctggaatacg
      661 agctaagcga gccggccagg atagtcgacg aggcttacct ggcccggtcg gaggccgacc
      721 ctgacgacgg ctcggccctc gtcgcgcagg tcatcagatc cccgcgctcg aggtcggccg
      781 aggaaggcgc ctgggacgcc gaacgatggt tcgtcgacga cttctgcgag ggccccggta
      841 acgtgctgta cgtccatccc gacggcgccg tggcggtctg ctgcggcttc gccaacgaga
      901 acccgcagct tatcgtcggc tcggtcaacg actccgtaga agcgattgtc gccgaggcgg
      961 ccgcccgccc gctcgtgcgc gcctgctacg aggccggcct cgccacgacg aggaggcggc
     1021 tcgaggcggc gggagtccgt ttccctggca agaccggcga cgcctgcttc ttctgcgact
     1081 acctgtgcaa gcgaggcctc gtaggctctc gtgggtgacg aacctcagtc cgagtctatc
     1141 tcgaagcgtt ctccgagcac ggccaggccg acgcggtcct cgatgtctag cttcttgtag
     1201 gcccgggcca gcgtgttcct gaccgtcgac tcggacagct cgaagtcgat ggatatctcc
     1261 ttctgcgact tgccggagag cgtctggcct atgaacgccc gctcggccgt cgatagtccc
     1321 ttctccgcca gcgatagctt gggcttcggc tccttgagga tgataacgag cctatctttg
     1381 tagaggaacc acaggaagac gccgaacgcg acgatgaaga aggtaggggc gctcgcttcc
     1441 atcggagatt tgccgctact aacgacgccg actatttcta tagcgaggag ataactgatg
     1501 ctgagtatca gcttaggcaa gcgatgcctc gcgtagaagc cggtgcgttc gagcaacaga
     1561 atgccgatga taaaaaaacc gagtccatag atgtttctcg tgctttggat aatggaaacg
     1621 gggctaaaga ccaggaagac tataggctgt aggattcgaa gcggcctaac caaggcgctt
     1681 agaatgaacg ccgcgcatat cgccaactgg gcgagttggt aggggttcgt cgtcgaccag
     1741 aagaaaccat gcttcccgat gtcggagacg aagttgatca tgaacccgcc aaggaagaac
     1801 gcggcgaata gaaacccggt cgaacgtagc ttatcgttgc tcatctggtg tttcccaaaa
     1861 tgtatatata gcttcatcag ccgttaggat cgaatcgatg acggctgatc cattcttttc
     1921 cgagcacgat gaaacggctg atcgctcagg ctcgaccgta tcgccgttta cgggattgta
     1981 ctcgcccgtc aggcggtcga tctcggcgtc cctgcggtag ccgctcgtca tgcatagcgc
     2041 gaggcgcgcc aggccctgct cgagcgccgc cgagtcgcct cccttgaagt ccgcgatggt
     2101 gcggagacgg gccatcgggt tggcgtcgtc ggccggcgcg tggtcgaaga atcggcgggc
     2161 gcggccccgg ttggcgatca cgaaggtcgc caggtggctg agcgacgagc gcatcgcgtc
     2221 gtcgcagccg gctgcgaagg gcaggagccc gacgagcttg gctaccttgt tgttcttgaa
     2281 ggcgcgtgtt tccgaatcgt tcatgcggag ggcttcggcc gaaacatcgg ataaacgatt
     2341 ccacaaggaa tcgattgttg caaagttcat gcgctgagta ttcgagagaa tcgatggggc
     2401 gtcaaggcat ttttttgtac catctccgtt cggaaccgta gccgacggaa actggattct
     2461 ggcgcttgag cggacgaggc gcttcgacgt atcatggccg catgaagccc gcaggcggac
     2521 agccgtatcg agctaccttt ctcttcgtcg ccataccgat cgcggcggcg atagcgctgg
     2581 cctggattgt catcgacaag gcgtccgtgc gcacgcacct cgagatggcc gagcgtaatc
     2641 gcgccctgca ggtcgaactg gcgcgtaacg cgatggaaga gggcttcgag cgcttgctcc
     2701 aggagaaccg cgttctggcc gcgtattcgt tccccgagta cgcgcgtggc ctgcgcggca
     2761 gggcttccat ggaggccttg ctcgaagccg agggcgatgc gtatcccgag tcgctatcgt
     2821 actgctacct gaccgggcca ggcaggcttg agctagcctg ggcgcggccg ggagccgagg
     2881 agaccatgga ggccctggtc ttcgcctccg tcgcccaatg ggatggcttc ctgggcatga
     2941 gcgacccggt cgtgctttca agccggtcga atcggcccga gccgtatttc atggcgctgt
     3001 tcccggtgcg cgtcggtggc gacatggtgg gtatcctcgc gaccgctatc ggcctcggac
     3061 gcagcatcga cacgtacctg tcgccgctgg ctgaaggcga ggggcggcgt tcgttcctga
     3121 tgttcggggc gggtcgcgta ctctgggcct ccgacgatcg taacccgagc ctcttcgagc
     3181 tggaccaggg cagcctgatg acctcgcgct cgttcgcgct cggcgatggg gagttcacga
     3241 tcatagccga cgagtcccgc gcgtccctat tgtccgagct cgaggccatc gataggccgc
     3301 gcttcctggt cggcgccatg ggactgctga ccatggtcgc cgctctgttc ttcgcgaacc
     3361 gcctgtacct cgagcgccgg gggcggcagg ccctcgcgag cgaggagcgg cgcctgtccg
     3421 ccagggtggc ggccggcgag cgggagctca gggccttgct cgatgaccgc gagctgctct
     3481 tacgggagct ccatcatcgc gtgaagaaca acttccagta cctggatagc ctcatcgagc
     3541 tacagaaggg cggcgcctca gccgatgcgg ccgccgcgct atcgaatatc caggctcgga
     3601 tagccgcgct cgccgccgcc tacctgatca acgccgatcg cccggagtcg ctccgggtcg
     3661 atgcccgcga ctacctgacc gccctggcgg gcaaggtcgt cgatggagcg ggcgtcgagc
     3721 tcgtcataga ggccgaggac atacctgtct ctctcgattc ggcggtgccg ctcggtctcc
     3781 tgttccacga gctcctgacc aacgccgtac gccacggtta cgccgggaaa cgcggctatg
     3841 ccggggggca cggagacagg gtagctgtcc gattctaccg agacgggggg acggcggtgc
     3901 tcgaggtcag ggatttcggt accggcctcg aggccggtac ggcggacggc ctgggcctga
     3961 cgatagtccg ggccctcgtc gcgcagctca atggcggttt cagcctcgag aatcgagagc
     4021 cgggcgtcat cgcggaagcc cgattccccc tggcctgacc cggaaaagcc tctacgggca
     4081 tctctacgag cgccatgggc agagcagccg ctcggccagg tccaccgcca tgaagaagcc
     4141 gagccccagg aggcttagcg cgacgatggc ggcgtacatc tccgcgtacg ccaccctggc
     4201 ccaggcgtcg acgatcaggt agccgagccc cgtctcggta gcgaaggtct cagcgaggaa
     4261 cagcaccgcg accgccgtgc cgaggctgac tcgtagcgag gtcagcagat cgggcagcgt
     4321 cgacggaacg acgacccgga acgccaggtc gagccgggac gcgccgagtg agcggaccga
     4381 gtcgacgagc ggccgcggta tgcgtctggc cgcgtcgcgg gccgccacga gtatctggct
     4441 gaagacgatc agcgcgacga ggatgacctt cgaggcctcg ccgagcccga agaccagcat
     4501 gatgacgggc aagaaggcaa ccttgggtag cgggtggagg acgtagacga ccggagcgac
     4561 gacggcgtcg acccggcgcg agcggccggc cgccagcccg agggccgccg ccggaaagaa
     4621 gctcgcggcc agggcccaga gtacgcggct ggcgctggcg gcggcatgcc gaccgagcga
     4681 gccagtggcg gccaggcggc cgaacgccgc gaaggtcgtc gccgggctcg gcaggaaggg
     4741 tttcccgagc gcggcggcgg ccgccgacca ggcggcgacg atgaccgcga cggccagcgc
     4801 gatggaggcg gcccttctcg tcgccatggc gccgttccgc cctccgagcc tcatcttcgc
     4861 gcctccggga tcggcgccat tccggggctc gccgagtcgt cggtcgggcc ggagcccctg
     4921 agcgccgagc ggatcgcgcc gcatagctcc aggtagcgcg aggccgacct gaagtccccg
     4981 gcggcgcccg gcgcgaagcg gacccgagcc gcgttctcgg gaatgtcgaa acgggcgcgg
     5041 atggttccgg ggttgcgacc gcccatcacg aagaccgcct cggccagata ggccgcctcc
     5101 tctatcgcat gggtcacgac gatgatcgtc atcggtctcg agcgcctgac ctcgagcagg
     5161 gcttcctggg cggcctcgcg tgtcagcgcg tcgaggctcg agaaaggctc gtccatcagg
     5221 agcaggtcgg gctcggaggc cagggcgcgg gcgatcgcga cgcgctgctt catgccgccc
     5281 gagaggcggc tcgggtagaa gcccccgaag gaagacaggc cgagctcatg gagtatgggg
     5341 cctacccggc gcctccgctc cgaccggccg atgccgcgca cggccagcgg cagttcggcg
     5401 ttgccctcga ccgtcagcca ggggaggagg ccgtagtcct ggaagatcac cgcggtacgg
     5461 ggccgtacgc cgcgtaccgg ctcgccgtcg atcagcgcct cgccggcgct cggcgcgagc
     5521 agaccggcca tcgcgcctac caggctcgac ttgccgcagc ccgacggacc gacgatggcc
     5581 gagatcgcgc ccgagccgaa gacggcgtcc acgcccgcga cggccggaac gcggggtccg
     5641 gggcggccga gccgcccggt ccatccgggc gatgagccgc ccgggtagtc tatagtcagc
     5701 tcacggagct ctaccacgct gcgacggccc tgtcgtcgag cagggctgat ggatcgacgg
     5761 gtttggagag cagattccgc tccctgagcc acgatacggc cgcctcgacc tggccgagcg
     5821 gcggcatggt aggcttccga taggtcacga agcggtacgc gccggcggca tcctcgggga
     5881 agcgggcctt ctcgacgagg aaggcgcggt acgactcggg cgccgcgttg acgcggcctg
     5941 aggcgtcggc gtaggcctcg tagagcctgc gcacgtcctt gagcctggcg ttcagcacgg
     6001 cctcggagaa cacgacgact gccgcgtcga tgtggaactg ttcggtcgtg ccgaccaacg
     6061 tggcgccgcg ctcgatggcc gtggtcagga agggctcggg taggccggcg gcgtccactt
     6121 ggcccgcgag cagcatctcc atgcgtaccg gcatcttggg tatggccacg gccgtatagg
     6181 cgtccggggc gaggcctgag gctccgacga tggcgtcgac cgagtactgg atgatcgtgt
     6241 tggtggacag cccgacgcgc ttgccggcta actcggccgc cgagcccagg cccgaaccgg
     6301 gggccgcggc gatgccgtag cgaccgtcgg tcgcgctcgt gatcctcgtg tcaaaaccgc
     6361 cggccgcgag aaaggccgcg gcgagtacgt ccgagacggc gccgtctagc cggccggcct
     6421 ggatggcggc gtcgcgctcc tgcgcgttcg cgaaactgac gagttccacc tcgacgccgc
     6481 gcttctcgaa caggccctcg gcctcggcga cgaggaaggg caatgagtcg gcgtccggca
     6541 tgatgccgag ccggagcggc tccgcccctc gcttcgggga acaggacgag gcggccacgg
     6601 cgaacagggt cgctacggcc aaggcaagcg acgctttgag aatgggattg tgtacatgca
     6661 tcggaggctc ctgtcggcgt ggcgccggac gaccgcgatc gatcgtcagg cgccgcgccc
     6721 gagcctcaga gtctaccgga gcccggctcc taaggcaata accgccttag aagctggcca
     6781 tccgcgagac gagattgatc aggaacacct gtccggtcgc tggcgcgtcg ggtatctcgt
     6841 aatagccgta gtagcagtac tcgccctgcc tgacgaccgg gaagtacgtc ccggagtacg
     6901 cgtcgcccgc gtagatgtag ccacctgtcg ggttggctcc ggatgagata tagatcccgc
     6961 ggcgccccgt gtccgccgag aagatattga ccgccaccga cgactcccgg tacgagttgt
     7021 agagtacgcc gtagtatagc ggcgtgtacc agatgtcctc cgaggcggac gaggcgacgg
     7081 tcttggacga gcgaatggcg ttgaggccca tctggttgcc cgagtctata tccgacggct
     7141 gggtcccggt cagggcccat gcgctccagt tctggtcgac gcggtagatg aagtaggcgc
     7201 cacccgtacc catcgcgatg gttcccttgc tcgaagccgc gatgttcctg acgcgcccgt
     7261 cgttggtcga ggccgtcgtg aaggaagagc cgtgcgtgac gatgatcggg tcgccgtaga
     7321 tcctcgcttc cgtcgagtat gtcgcagaga tcaggtcttc cggcagtagc gtgacgctcc
     7381 aggtcggcat ggcgccgtag atgttggcca gtcccgtgat gccctggtcg tcctcaagta
     7441 tcgccttgag cgcctccgct cgagtcacgt ccgccggctc gtcatcatcg tagacgaccg
     7501 tgacgttcga cg
//
LOCUS       DAOOLN010000005         7136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_12457_length_7136_cov_3.018641, whole genome shotgun sequence.
ACCESSION   DAOOLN010000005 DAOOLN010000000
VERSION     DAOOLN010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7136
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12457_length_7136_cov_3.018641"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /gene="tmk"
                     /locus_tag="PLW80_00150"
     CDS             complement(<1..624)
                     /gene="tmk"
                     /locus_tag="PLW80_00150"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPB64938.1"
                     /translation="MQQTRTILEGFVALEGIDGAGTTTQLRRLSARLEAAGVAFSTEC
                     EPTPGPVGRLIRQALSGAFDARPETVARLFAADRGEHLYGPGGILERTAAGSLVVSDR
                     YLFSSLAYQGLTCGPDLPAELNAPFPLPELLLFFELDPEIAAERMAARDSLEIYENLA
                     FQRRVDAAYRAVVSSFEGSGMAVVRVDAAASPDEVERTLWSAIEPISM"
     gene            692..5062
                     /locus_tag="PLW80_00155"
     CDS             692..5062
                     /locus_tag="PLW80_00155"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HPB64939.1"
                     /translation="MKLRAPAPIVIAAIVVLAVSAAWAVVAPPLLGRMESAAAALIER
                     EAAYAIRGLGLSASYRAASRRLLGSIELRGVELRGGESVVARVRRLQLRYGLGALLGG
                     RFELTGVRVDGVSMAAELQDAKALVERLASALPGTGSSASEGAASLAVELRRLTLTLE
                     LGDGATATLSLRAADLLLDGPGGVRASAAGSIRASDPSGRYLATAVELPFSCTASVRS
                     PGGQGSGLEASIAAVIAGDSDLGRLSRARIDATLDDTGLSASVEPGAGIERLRFDWNP
                     STGLAKLDLAVTRWSPASLFYPAGRLSGARPWLESSYSGSLRVSSDLSAEGTSVAADL
                     RGVIPLALPGGRPRATLSASGGWDTLRVRSATLRNGSLGAEFSGELSLGRLSADGRLS
                     ASYALGPGLEASADFQLSGAGRSWFAYAPAIRAADATLGDAIVSLELSSSSGALDQAS
                     FYLDASLPTIADADGDPAVEPVDSPGIVEASSARAASRLTIEGSASLGEEPYLEAVAR
                     VGTLSLEAFPGLLTALVGAVAADALAPLSVGGELSLFSDLSGLSYNSSGLLVVYDGAI
                     KGFGMLGFSGGLDRLRISSIDATIGGYSVKGSASLEYGASSGPSFAADLRVEDVPYGL
                     SGAMADGAVFVSGDYGLRFVGSGRDDEYVASLDIDQMPIPLFGSVSFLSAAADGRFSS
                     TDDWNLVFESLALEQPYGSASRLPSVSLAGAFDESGGRVGSLGYRDAVSSLNGSATVT
                     WTLSGGFRVSGNARMAGPAGEYYALDGEYASDGTMDAALSTRKAPLARLYLPWLRGVV
                     DADARIGGSIRDPSARFSFTVNGGQRADNLPFAAGAGTFERGVLSLVDSRVRLGDQAV
                     SGLTLSYGVGDAAASLAANVELAMGRNRLTGRLVAVGASNGAGPSPFGDYLVAGELSG
                     ASWTGGELGDIPFSLGLADGDASLSMGPSGELEASLGAAGELRASLDRSMPISLEAQG
                     VLADGVINVDVRDAVVDLPFLFKLIGLPMLGADSGVGRGAVTIRGKAVDPSVEGSFEL
                     EGFYLSLPDYVSEPIGPLEAPLFFTGRTMETLQTDVPCGDAVLTVALEGVLNQGIPDD
                     VHLTVSTSGLGLVPVSTRLLGLDIDGLARPELVIDANRDRSRMTGSIMLASGDVVITP
                     GVVSGRMQASRGAGILEPVEFTGALDISFGKAVRVYFPDKRLPVIYGLTDPSSRLSVD
                     FDAALGEFNLDGQAVLRGGSVFYIQRNFYLKHATIDFDEDAEQFDPLINAEAETRTSS
                     ESGPVVVTLRAVDSRLSSLSFSLESSPSMAEDTILQMLGQNLIGAQGGSADLGRFVFE
                     NSDLIPQLNVMSVLERNLHGLLGLDLFVVRSQVFQRWLYDVSGLSGSSGASSLADYLD
                     ETAIVGGKYIGDKLFAQLALSLVADPLASSAALSLDSEFSLEWKAPHFTLNWSIEPEH
                     PDSLFIEDQSFSFLWRIPLQ"
     gene            5077..>7136
                     /gene="bamA"
                     /locus_tag="PLW80_00160"
     CDS             5077..>7136
                     /gene="bamA"
                     /locus_tag="PLW80_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HPB64940.1"
                     /translation="MKRFVASLALLALGAVAFAQAEPDWYVGKTIKDIRFDGLVVVPM
                     KDLEPIVKEFRGKPFDDQLWMNLLARVYETDYFDAVAPEAVPSDQAATAVIILFKVKE
                     KPAVTSVAIVGNSGIRTQEILDALSVKERTIFNPSRMRLDEISLRRLYQEKGFPEIKV
                     TAASSTLADGGVAVTFTVAEGVQNIVESIRFEGLESVSESSLKGEIKLKEKGLFQSGQ
                     FSEGKLDESRRAIEAFYKKRGYVDAKVSDVRRESAASDKGAMRLTLIFVIDEGRRYTY
                     GGMGFAGNSLYTSEQLGALVRQQPGAVLYYERLLQDQSRAADLYFENGYIFNGFRLAE
                     TRDEELGSIAYTLVIDEKPQAHIEDVIFRGNVKTKEFVLRRLVPLESGDVFSKAKVLE
                     SLRNLYNTQYFSSIVPEYEQGSQDLFVDLIISVEEQSTASIQFGMTYSPGTSAGEFPI
                     IGLVNWSDINFLGNGQTVALKTNLAVDSQDLTFSFSDDWLFGKRLSGSLEFAFEHEYL
                     EAAQDSTGVIFPYGDPTRVPDPYNSYEEYVAAGKLVPDGYKMTYDTWTFSLGYSGGYR
                     WNTKLGTAGVVPGVTHELGMKTYDSDLYRPYDSTISENLDTWLFSNTVYARAYLNKLD
                     LWYDPSRGYYASQRLGLTGWFDGEINHFLRSDTRADAFVTLFDLPITESYSFKGVLGA
                     HSKFS"
BASE COUNT         1124 a         2403 c         2363 g         1246 t
ORIGIN      
        1 catcgatata ggctcgatcg ccgaccagag cgttctctcg acctcgtcgg gcgacgcggc
       61 ggcgtcgacc ctgacgacgg ccatgccgga gccctcgaag gacgagacga ccgcgcggta
      121 ggcggcgtcg acccggcgct ggaacgcgag gttctcgtag atctccaggg agtctcgtgc
      181 ggccatgcgc tcggcggcta tctccgggtc gagctcgaag aataggagta gttcgggtag
      241 cgggaaggga gcgttgagct cggccggcag gtccggcccg caggtcagac cctggtaagc
      301 cagcgacgag aagaggtagc ggtcgctgac gacgaggctc ccggcggcgg ttcgctcgag
      361 tatgccgccg ggaccgtaga ggtgctcgcc gcgatcggcc gcgaacaggc gggccaccgt
      421 ctcgggccgc gcgtcgaagg cgcccgatag cgcctgtctg atcaggcgcc cgacggggcc
      481 gggagtcggc tcgcactcgg tcgagaaagc gacgcccgcg gcttcgagcc tggccgagag
      541 gcgccggagc tgggtagtcg tgccggcgcc gtctatgccc tcgagcgcga cgaagccttc
      601 cagtatggtt ctagtctgtt gcatcggaga gggagcatag ccccgaggcg cgatatggcg
      661 caaggcccgg cgctgtggta ctgtggatcc cgtgaagctt cgagccccgg cgcccatcgt
      721 catagccgcc atcgtcgtac tcgccgtgtc agcggcctgg gccgtcgtcg cgccgccgct
      781 gctcgggcgc atggagtcgg cggccgcggc cctgatagag cgtgaggcgg cctacgccat
      841 tcggggactc gggctgtcgg cctcctaccg ggccgcctcg cggcgcctcc tcggctcgat
      901 agagctacgt ggagtagagc tgcgcggcgg cgaatcggtc gtagcgcggg ttcggaggct
      961 ccagctacgt tacggactcg gcgccttgct cggcggccgg ttcgagctga cgggcgtacg
     1021 cgtcgacggc gtgtcgatgg cggccgagct ccaagacgcg aaggccctcg tcgagcggct
     1081 agcgtccgcc ctgcctggaa ccggatcctc ggcgtccgag ggggcggcta gcctcgcggt
     1141 cgagctacgc aggctgaccc tgaccctcga gctgggcgac ggtgcgacgg cgaccctgtc
     1201 gctgcgggcc gccgacctgc tgctggacgg ccccggcggc gtacgggcga gcgccgccgg
     1261 gtcgatacgc gcctccgatc cgagcggtcg atacctggcg accgccgtcg agctgccgtt
     1321 ctcgtgcacc gcctcggtac ggagccccgg cgggcagggc tccgggctgg aggcgagcat
     1381 cgccgcggta atcgccggcg acagcgactt gggccggcta tcgcgcgcgc ggattgacgc
     1441 gacgctcgac gataccggcc tgagcgcgag cgtcgaaccc ggcgcgggga ttgagcggct
     1501 acgcttcgac tggaaccctt ccacggggct ggctaagctc gacctggccg tcacgcgctg
     1561 gtccccggct tcgctgttct atcccgcggg tcgcctgtcc ggcgcacggc cctggctcga
     1621 gtcgtcgtac tcgggctcgc tccgcgtctc gagcgacctg agcgccgagg gcacgagcgt
     1681 cgcggcagac ctgcgcggcg tcatcccgct cgcgttgcca ggcgggaggc cgcgggccac
     1741 gttgtcggcc tcgggcggct gggacaccct gcgcgtgcgt tcggccaccc tgcgtaacgg
     1801 ctcgctcggc gccgaattct ccggagagct gtcgctcggg cggctgagcg ccgacgggcg
     1861 gctatcggct tcctacgcgc tcggtccggg cctcgaggcg tcggccgatt tccagctgtc
     1921 cggggccggg cggtcgtggt tcgcctacgc cccggccata cgcgccgcgg acgcgacctt
     1981 aggcgacgcg atcgtatcgc tcgagctgtc ctcgtcatcc ggagcgcttg accaggcctc
     2041 attctacctc gacgcgtcgc taccgactat cgccgacgct gatggtgatc cggctgtcga
     2101 gccggtcgac agcccgggca tcgtagaggc ctcgtcggcg agagccgcct cgaggctgac
     2161 catcgagggc tccgcctcgc tcggagaaga gccctacctc gaggcggtcg cccgcgtcgg
     2221 caccctgtcg ctcgaggctt ttcccgggct cctgaccgcc ttggtcggcg ccgtcgccgc
     2281 ggacgcgttg gcgccgctca gcgtcggggg cgagctgtcg ctgttcagcg acctctccgg
     2341 gctgtcctac aactcgtcgg gcctgctcgt cgtttacgac ggcgcgatca agggcttcgg
     2401 catgctcggc ttctccggag ggcttgaccg actgcggata tcgtcgatcg acgcgacgat
     2461 cggcggctac tcggtcaagg gctcggcctc gctcgagtac ggtgcctcgt ccggcccgtc
     2521 gttcgccgcc gatctacgcg tggaggacgt accctacggg ctatctggcg ccatggcaga
     2581 cggggccgtc ttcgtctccg gggactacgg cttgcgcttc gtcggtagcg gacgcgacga
     2641 cgagtacgtc gccagcctcg atatagacca gatgcccata ccgttgttcg ggtcagtatc
     2701 cttcctgtcg gccgccgccg acggccgctt ctcctcgacc gacgactgga acctcgtctt
     2761 cgagtcgctg gcgctcgagc aaccctacgg ctccgcctcg cgcctgccgt cggtctccct
     2821 agcgggcgct ttcgacgaat ccggcggccg cgtcggttcg ctcggctatc gggacgccgt
     2881 atcgtcgctc aacggatcgg ctaccgtaac ctggaccctg tcaggcggtt ttcgagtgtc
     2941 gggcaacgcg aggatggccg gccccgccgg cgagtattac gcgctcgacg gcgagtacgc
     3001 tagcgacggg accatggacg cggcgctatc gacgaggaag gcgccgctcg ccaggctgta
     3061 cctaccctgg ctacgcggcg tcgtcgatgc cgacgccagg ataggcggct cgatacggga
     3121 tccatccgcc cgcttcagtt tcaccgtgaa cggcggtcaa cgcgccgaca acctgccgtt
     3181 cgcggccggc gccgggacct tcgagagggg agtgctcagc ttggtcgatt ctcgcgtccg
     3241 cttgggcgat caagcggtct ccggactaac gctcagctac ggcgtcggcg acgccgcggc
     3301 ctcgctggcg gcgaacgtcg aactggccat gggccggaac cgactgaccg gtaggctggt
     3361 cgccgtaggc gcctccaacg gcgccgggcc gtcaccgttc ggcgactacc tagtcgcggg
     3421 cgagctgtcc ggggcttcgt ggaccggcgg cgagctgggc gacataccgt tctccctcgg
     3481 gctagccgat ggcgacgcga gcctgtcgat gggcccgtcc ggcgagctcg aggcctcgct
     3541 cggagccgcc ggcgagctca gggcgagcct cgatcgttcc atgccgatat cgctcgaagc
     3601 ccaaggcgtg ctcgccgacg gcgtgatcaa cgtcgacgtt cgcgacgccg tcgtcgacct
     3661 tcccttcctg ttcaagctga taggcttgcc gatgctcggc gccgactccg gcgtcggccg
     3721 gggggccgtc accatacgcg gcaaggccgt cgatccgtcg gtcgagggct ccttcgagct
     3781 cgagggcttc tacctgtcgc ttccggacta cgtatccgag ccgatcggcc cgctcgaagc
     3841 gccgctgttc ttcaccggcc ggaccatgga gacgctgcag actgacgtgc catgcgggga
     3901 cgcggtcctg accgtcgcgc tcgagggcgt cttgaaccag ggcatacccg acgacgttca
     3961 cctgaccgtc tctacctcgg ggctagggct cgtgcccgtg tccaccaggc tgctcgggct
     4021 cgacatcgac ggcctcgcga ggccggagct ggtcatcgac gccaaccgcg accgatcgcg
     4081 catgaccggc tcgatcatgc tggcttccgg cgacgtcgtg atcacgcccg gggtcgtcag
     4141 cggacggatg caggcgagcc gtggtgccgg gatcctcgag cccgtggagt ttaccggagc
     4201 cctcgatata tcgttcggca aggcggtccg cgtctacttc cccgataagc gcctgcccgt
     4261 catctatggc ctgaccgacc cgtcgagtcg gctctcggtc gatttcgacg cagccctcgg
     4321 cgagttcaac ctcgatgggc aggccgtgct acgcggcggc tcggtcttct acatccagcg
     4381 taacttctac ctcaagcacg cgacgataga cttcgacgag gacgccgagc agttcgaccc
     4441 gttgatcaac gcggaggccg agacgaggac cagctcggag tcgggccccg tcgtcgtaac
     4501 gctccgcgcg gtcgacagca ggctatcgag cctatccttc tcgctcgagt cgtcgccgag
     4561 catggccgag gacacgatat tgcagatgct cgggcagaac ctcatcggcg cccagggcgg
     4621 ctccgccgat ctcggccgct tcgtcttcga gaatagcgac ctcatacccc agctgaacgt
     4681 catgtcggtg ctcgagcgta acctacacgg cctcctcggc ctcgacctgt tcgtcgtgcg
     4741 ctcgcaggtc ttccagcgct ggctctacga cgtatcgggg ctttccggct cgtctggcgc
     4801 ctcgagcctg gccgactacc tcgacgagac ggccatcgtc ggcggaaagt acataggcga
     4861 caagctgttc gcccagctgg ccctgtccct cgtcgccgat ccgcttgcat cgagcgccgc
     4921 cttgagtcta gactccgagt tcagcctcga gtggaaggct ccgcatttta cgctgaactg
     4981 gagtatcgaa ccggagcacc cggactcgct gttcatagag gatcaatcat tttcgttcct
     5041 ttggagaatc cccctacaat gaggaaggcc atcgatatga agcgatttgt agcttctctc
     5101 gcgctgctag cgctcggcgc cgtagcgttc gcccaggcgg aaccggactg gtacgtcggc
     5161 aagacgatca aggatatcag gttcgacggg ctcgtcgtcg tcccgatgaa ggatctcgag
     5221 cccatcgtca aggaattcag gggcaagccg ttcgacgacc agctctggat gaacctcttg
     5281 gccagggtct acgagaccga ctatttcgac gcggtggccc ccgaggccgt gccgtccgac
     5341 caggcagcga cggccgtcat catcctgttc aaggtcaagg aaaagcccgc cgtgacgagc
     5401 gtggcgatcg tcggcaactc cggcatacgt acccaggaga ttctggacgc cctgtcggtc
     5461 aaggagcgga ccatcttcaa cccgtcgcgg atgcggctcg acgagatatc gctgcgaagg
     5521 ctgtatcagg agaagggctt ccccgagatc aaggtgacgg cggccagctc gacgctggca
     5581 gacggcggcg tcgcggttac cttcaccgtc gccgagggtg tccagaatat cgtcgagtcc
     5641 atcaggttcg agggcctcga gtccgtctcc gagtcgtcgc tcaagggcga gatcaagctc
     5701 aaggagaagg ggctgttcca gagcggccag ttcagcgaag gcaagctcga cgagagccgg
     5761 agggcgatcg aggccttcta taagaagcgc ggctacgtcg acgccaaggt ctccgacgtg
     5821 cgtcgcgagt cggcggcgag cgacaagggc gcgatgcgcc tgaccctgat cttcgtgatc
     5881 gacgagggcc gacgctatac ctacggcggt atgggcttcg ccggcaactc gctgtacacg
     5941 agcgagcagc tcggcgccct cgtccggcag cagccaggcg ccgtgctcta ctacgagcgc
     6001 ctgctccagg accagtcgcg cgcagccgac ctctacttcg agaatggcta tattttcaac
     6061 ggcttcaggc tcgctgagac ccgcgacgag gaactcggct ccatcgcgta cacgctcgtg
     6121 atcgacgaga aaccgcaggc ccacatcgag gacgtgatct tccgcggcaa cgtgaagacg
     6181 aaggaattcg tgctacgccg gctcgtgcct ctcgagtcgg gcgacgtgtt ctccaaggcc
     6241 aaggtgctcg agagcctgcg caacctgtac aatacccagt acttctcctc gatagtgcct
     6301 gaatacgagc agggtagcca ggacctcttc gtcgacctta tcatcagcgt cgaggagcag
     6361 agcaccgcgt cgatccagtt cggcatgacc tactcgccag gcacgagcgc aggcgagttc
     6421 ccgatcatcg gcctggtcaa ctggagcgac atcaatttcc tcggtaacgg gcagaccgtt
     6481 gcgctcaaga ccaacctggc ggtcgactcg caggacctga ccttctcgtt cagcgatgac
     6541 tggctgttcg gcaagcgcct gtccggatcg ctcgagttcg cgttcgagca cgagtacctg
     6601 gaggccgccc aggactcgac cggcgtcatc ttcccctacg gcgacccgac gagggtaccc
     6661 gacccgtaca acagctacga agaatacgtg gcggcgggca agctcgtgcc ggacgggtac
     6721 aagatgacct acgatacctg gaccttcagc ctcggctact cgggcggcta ccgctggaat
     6781 accaagctgg gtaccgccgg cgtcgtgccc ggggttaccc acgagctggg gatgaagacc
     6841 tacgattccg atctgtaccg gccctacgac tcgactatct cggagaacct ggacacctgg
     6901 ctgttctcca acacggtcta cgcccgcgcc tacctgaata agctcgacct atggtacgac
     6961 ccgtcgagag gctactacgc cagccagcgc ctcggcctga ccggttggtt cgacggggag
     7021 atcaaccact tcctgcgtag cgatacccgg gcggacgcct tcgtcacgtt gttcgacctg
     7081 cccatcaccg aaagctattc cttcaagggc gtactcggcg cccattcgaa gttctc
//
LOCUS       DAOOLN010000006         6573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_13959_length_6573_cov_2.943388, whole genome shotgun sequence.
ACCESSION   DAOOLN010000006 DAOOLN010000000
VERSION     DAOOLN010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13959_length_6573_cov_2.943388"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PLW80_00165"
     CDS             complement(<1..529)
                     /locus_tag="PLW80_00165"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPB64941.1"
                     /translation="MMDKPTTAAPRRHAPALRPEFVPLGFIVERPSYGYEVYRRFSDS
                     LSGLWGISESQMYATIKRLEDRGLVVHLAERDIADEPDPVGERRPRRGPGAEKRLLAP
                     SEAGRATFDAWLAEPTPTSPRVLRLEFVTRLFFASRSDPSAGAALFAAQRAAVVESIG
                     RLRDGMAGSRAVDAAG"
     gene            649..1845
                     /gene="trpB"
                     /locus_tag="PLW80_00170"
     CDS             649..1845
                     /gene="trpB"
                     /locus_tag="PLW80_00170"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HPB64942.1"
                     /translation="MGDYFKSFPDASGNYGDFGGSFIPPDLQREMDKITDAYYSISKS
                     HEFISELRGIRKHFQGRPTPVYFAKTLSDECGGRIYLKREDLNHTGAHKLNHCMGEAL
                     LATYLGKKKLIAETGAGQHGVALATAAAYFGLECEIHMGEVDIAKEAPNVTRMRILGA
                     TVVPVSAGLKTLKEAVDSAFSAYLEDPVSSIYCIGSVVGPHPFPMTVRDFQRVVGIEA
                     REQFFEMTGEHPDDVVACVGGGSNAMGIFSAYLDDEGVRLYGVEPMGRGPETGDNAAT
                     ITYGKPGVIHGFKCYLLQDEKGEPSPVYSIASGLDYPGVGPEHSMLHDLGKVRYVSVG
                     DDECVDAFYELSRKEGIIPALESAHAVAYAKRLAKADPRRSILVNLSGRGDKDIDFVI
                     AKYGYR"
     gene            1940..3424
                     /gene="pepD"
                     /locus_tag="PLW80_00175"
     CDS             1940..3424
                     /gene="pepD"
                     /locus_tag="PLW80_00175"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HPB64943.1"
                     /translation="MHIDELQPRSVWKRFFDICAIPHPSHREAALADALMRWAAEHGI
                     EAKRDEAGNVAMSRPAVFGMEDHPGVILQAHLDMVPQKAAESGHDFERDPIRPRLDPK
                     NPAWLVADGTTLGADDGIGVAMALALLEDHELRSGRLECLFTVNEEDGMSGARAIEPG
                     FVTGSLLLNLDGEDEAELTIGCAGAVRTIAELSIPATAAPDGLAWFEAAVDGLLGGHS
                     GVDIDKGRANATLALVGILARTRAELRIASISGGTAANAIPREARAIVGVEAARAAEF
                     RAAFQSEATAARIALGDKDPGFAAALAPIAVKPAASLAPKDAASMLTMLGALPNGLVA
                     MEPDMPGLVRTSLNLGKAEGAVAPGTDRFRLETLVMIRSSSDDEKEALAATVERRLAG
                     VAEHGWAVEQRRPAVSPAWSPDPGSPLLGKAVAVYRELFGKDPRVTSTHGGLETGLFR
                     PRFPSWDMLSLGPTIRYPHSPDERVEIASVERSYRFARELVSRL"
     gene            complement(3513..4601)
                     /locus_tag="PLW80_00180"
     CDS             complement(3513..4601)
                     /locus_tag="PLW80_00180"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HPB64944.1"
                     /translation="MKIKELRLGRISVPLKKPFKTALRTVESVEDVIVELHADSGEVG
                     YGEAPPTGVITGDTTGAIIGAIQDHLSKAVVGLDVENLDEIMARIDASVVHNTSAKAA
                     VDIAVYDLFGKLCGQPLYKLWGGYRKEFATDITISANGAEQMAADAAEAVALGYRTLK
                     IKLGKDPAQDVARMKAIRAAVGYGVDLRVDANQGWKPKEAVRLIRAIEDAGLALELVE
                     QPVAAHDLEGLKHVTDNVSVPVMADESAFSARDAQRIMELRAADIVNIKLMKTGGVHN
                     ALRVCSLAEVYGVELMIGCMLEAKVSVTAAVHLAAAKKVITKVDLDGPVLCSEDPVDG
                     GAVFAESLVKPNEAPGLGITAIRGLRYL"
     gene            complement(4522..4941)
                     /locus_tag="PLW80_00185"
     CDS             complement(4522..4941)
                     /locus_tag="PLW80_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3870 domain-containing protein"
                     /protein_id="HPB64945.1"
                     /translation="MYDPSTIYVVGNAKTNTENAITTLYNSFYIGFVLDPATGRIVDA
                     SCSATIRTTDEFVRGIFVGKAMGRDDASLDDEVRRRYHGSSQKAILVAYKDAAKKLAE
                     IQARLGTEGPRNEDQGTAARPHIGTAQEALQDGAQDR"
     gene            complement(5008..6168)
                     /locus_tag="PLW80_00190"
     CDS             complement(5008..6168)
                     /locus_tag="PLW80_00190"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HPB64946.1"
                     /translation="MIQNALPYVAFLIFFAGLLVFLEKTLKLKVFKYVPAIVMLYFFA
                     MLLSTLGVWQKTPQITAAYKALKDGLLPAMIFLMLLRCDLRKIFKLGPRMLLGFFAAT
                     ITIMIGFIVTFAIFKGAFEPGSWKAFAALSGSWIGGTGNMVAIQGALDVPDANMGYTL
                     LIDSINYSIWVMFLLWVVSFAPAFNKWTKANSKVLDEVGQKLGAATEGNRTNVEFVDL
                     IVLLGTSLLVSALSIWLGGMLPKSVFFQSSTWTVLIATAAGVICAMTPLAKLPGSNHL
                     SNLMLYTIVGLIASRANFAELTQAPIYILAGFCILGIHAILLALVAKLCKLDLFTCGV
                     ASLANIGGVASAPILAASYGEALVPIGVLMALMGYIVGTGGGLLVGKVLSIL"
BASE COUNT         1161 a         2118 c         2219 g         1075 t
ORIGIN      
        1 agccggcggc gtcgacggcc cgggagccgg ccatgccgtc cctcaaccgg cctatggact
       61 cgacgacggc ggcgcgctgg gccgcgaaca gcgcggcgcc ggccgacggg tcggagcgcg
      121 acgcgaagaa cagccgggtg acgaactcga ggcgcagcac ccgggggctc gtcggcgtcg
      181 gctcggcgag ccacgcgtcg aaggtagccc ggccggcctc gctcggcgcc aggaggcgct
      241 tctcggcgcc ggggccgcgc ctcggcctgc gctcgccgac cggatccggc tcgtcggcga
      301 tatcgcgttc ggctaggtgg acgacaaggc cgcgatcctc gagccgcttg atcgtcgcgt
      361 acatctggct ctcgctgatg ccccagagcc ccgataggct atcgctgaag cgcctgtaca
      421 cctcgtagcc atacgaggga cgctcgacga tgaacccgag cggcacgaat tcgggacgca
      481 gggccggcgc gtggcgcctg ggcgccgcgg tcgttggctt atccatcatt gaacactagg
      541 ggcgggccgg ggaatctgtc aagtgaagcg atacagagcg ttcgttacta ttgacagata
      601 cgccaatcgt gggtatgatg cctctattgt ttctactagg agatacttat gggagactat
      661 ttcaagtcct ttccggacgc gagcggcaac tacggcgatt tcggcggtag cttcattccg
      721 cccgacctac agagggagat ggacaagatc accgacgcgt actactcgat cagcaagtca
      781 cacgaattta tttccgagct tcgcggcatc cgcaagcact tccagggccg gcccacgccg
      841 gtctacttcg ctaagacgct gtcggacgag tgcggagggc gtatctacct gaaacgcgag
      901 gacctgaacc ataccggggc gcacaagctc aaccactgca tgggcgaggc cctgctcgcc
      961 acgtacctcg gcaagaagaa actgatagcc gaaaccggcg ccggacagca cggcgtggcg
     1021 ctcgccacgg cggccgccta tttcgggctg gagtgcgaga tccacatggg cgaggtggac
     1081 atagccaagg aggcgccgaa cgttaccagg atgaggatcc tgggcgcgac ggtggtaccg
     1141 gtctctgcgg gactgaaaac cctcaaggag gccgtggact cggcgttctc cgcctatctc
     1201 gaggacccgg tctccagcat ctactgcatc ggctcggtcg tcggaccaca ccccttcccg
     1261 atgacggtgc gcgacttcca gcgcgtcgtc ggcatcgagg cgcgggaaca gttcttcgag
     1321 atgaccgggg agcatccgga cgacgtggtg gcctgcgtcg ggggcggctc caacgcgatg
     1381 ggcatcttct cggcctatct cgacgacgag ggcgtccggc tctacggcgt cgagccgatg
     1441 ggccgcggcc ccgagacggg cgataacgcc gcgaccatca cctacggcaa gccgggcgtg
     1501 atccacggct tcaagtgtta cctcctgcag gacgagaagg gcgagccgtc gcccgtctat
     1561 tcgatcgcga gcggcctgga ctaccccggg gtcggccccg agcactccat gctgcacgac
     1621 ctcggcaagg tgcgctacgt ctcggtcggg gacgacgagt gcgtcgacgc gttctacgag
     1681 ctcagccgca aggagggcat catcccggcg ctcgagagcg cccacgcggt agcctacgcc
     1741 aagcgcctcg ccaaggcgga cccgcgacgt tcgatactcg tcaacctgtc cgggcgtgga
     1801 gacaaagaca tagatttcgt catagcgaag tacggatacc gctgaagagg acggcgacca
     1861 tgggaggcga gcgccgtacg agccgccgct cgcctcctcc gagcatccgt ggtactatta
     1921 cggtggagga gtacaacgaa tgcacatcga cgagctacaa ccgagatcag tctggaaacg
     1981 ctttttcgat atatgcgcca tcccgcaccc gtcccaccgc gaggccgccc tggcggacgc
     2041 gttgatgcgc tgggcggccg agcacggcat agaggccaag cgcgacgagg cggggaacgt
     2101 ggcgatgagc cgccccgccg tcttcggcat ggaggaccat ccgggcgtga tcctgcaggc
     2161 ccacctggac atggtgccgc agaaggcggc cgagtcgggc cacgacttcg agcgcgaccc
     2221 gatacgcccg aggctcgacc ccaagaatcc cgcctggctc gtcgccgacg gcacgacgct
     2281 cggcgccgac gatggcatcg gcgtggcgat ggccctggcc ctgctcgagg atcacgaact
     2341 ccggagcggt cggctcgagt gcctgttcac cgtcaacgag gaggacggca tgagcggagc
     2401 ccgcgccatc gagccgggct tcgtcacggg ttcgctgctc ctgaacctgg acggcgagga
     2461 cgaggccgag ctgacgatag gctgcgccgg cgccgtcagg acgatagccg agctgtccat
     2521 cccggcgaca gccgccccgg acggcctggc ctggttcgag gccgccgtgg acggcctgct
     2581 cgggggccat tccggcgtcg acatcgacaa ggggcgggcc aacgcgacgt tggccctggt
     2641 cggcatcctg gccaggacgc gcgcggaact gcgtatcgcc tcgatatcgg gcggcacggc
     2701 ggccaacgcc atccctcggg aagccagggc catcgtcggc gtcgaggcgg cgagggccgc
     2761 cgaattccgc gccgccttcc agtccgaggc gaccgcagcc cgcatagccc tcggcgacaa
     2821 ggatcccggg ttcgcggccg cgctggcgcc catagccgtc aagccggcgg ccagcctcgc
     2881 ccccaaggac gcggcctcga tgctgaccat gctcggcgcg ctcccgaacg gcctcgtcgc
     2941 gatggagccg gacatgccgg gcctcgtcag gacctcgctc aacctcggca aggccgaggg
     3001 agccgtcgct ccgggtaccg atcgcttcag gctcgagacg ctcgtgatga tccgcagctc
     3061 gtccgacgac gagaaggagg ctctggccgc gaccgtcgag aggcggctag cgggcgtcgc
     3121 cgagcacggc tgggcggtcg aacagaggcg gccggcggta tcgccagcct ggagccccga
     3181 tcccggcagt ccgctcctcg gcaaggcggt agccgtctac cgggagctgt tcggcaagga
     3241 tccgcgcgtc acctcgacgc acggcggcct cgagacgggc ctcttcaggc cgcgcttccc
     3301 gagctgggac atgctgtccc tgggcccgac catacgctac ccgcactcgc ccgacgagcg
     3361 cgtcgagatt gcctccgtcg agcgctccta ccgcttcgcg cgggagctgg tctcgaggct
     3421 atgaagacgc catgagtaga atgggggcgc ctcggcctga gccggagcgc ccctcctaga
     3481 atccgctagc ccgcgacggc ggcaagcagc cgttacaggt agcgtaatcc ccgtatggcg
     3541 gttatgccga ggccgggcgc ctcgttcggc ttgacgagcg actcggcgaa gaccgcgcct
     3601 ccgtcgacag ggtcctcgct acagagtacg ggtccgtcaa ggtccacctt ggtgatcacc
     3661 ttcttggcgg ccgccaggtg tacggccgcc gtcacgctga ccttggcctc cagcatacag
     3721 cctatcatca gctcgacgcc gtagacctcg gccagcgagc agacgcgcag cgcgttgtgc
     3781 acgcccccgg tcttcatcag cttgatgttg acgatatcgg ccgcccgcag ctccatgatg
     3841 cgctgagcgt ccctggccga gaaggcgctc tcgtcggcca tcacgggcac cgagacgttg
     3901 tcggtcacgt gcttgaggcc ttcgaggtcg tgggccgcca ccggctgctc cacgagctcg
     3961 agcgcgaggc ccgcgtcctc tatggcgcga atgagcctga cggcctcctt gggcttccag
     4021 ccctggttgg cgtccacccg taggtcgacc ccgtagccga cggcggcccg gatggccttc
     4081 atgcgcgcca cgtcctgggc cgggtccttg ccgagcttga tcttcagcgt ccggtagccg
     4141 agagccacgg cctcggcggc gtcggcggcc atctgctcgg cgccgttggc gctgatcgtt
     4201 atgtcggtgg cgaattcctt gcggtacccg ccccagagct tgtatagggg ctggccgcac
     4261 agcttgccga acaggtcgta gacggctatg tccacggcgg ccttcgcgct ggtgttgtgg
     4321 accaccgaag cgtcgatgcg cgccatgatc tcgtcgaggt tctccacgtc caggccgacg
     4381 acggccttcg acaggtggtc ctggatggcg ccgatgatgg cgcccgtcgt gtcgccggtg
     4441 atgacgccgg taggaggcgc ctcgccgtag cctacctcgc ccgagtcggc gtgcagctcg
     4501 acgatgacat cctcgacgct ctcaacggtc ctgagcgccg tcttgaaggg cttcttgagc
     4561 ggtaccgata tgcggccgag ccgcagttcc ttgatcttca ttacggggtc cttccgttcc
     4621 cagcctggcc tgaatctccg ccagcttctt cgcggcgtcc ttataggcga ccaggatggc
     4681 cttctgcgac gatccgtggt agcggcgccg gacctcgtcg tcgagcgatg cgtcgtcccg
     4741 ccccatcgcc ttgcctacga agatgccgcg cacgaattcg tcggtcgtcc tgatcgtggc
     4801 cgagcaggac gcgtccacga tcctgcctgt ggcggggtcg agcacgaagc cgatatagaa
     4861 cgaattatag agcgtcgtta tcgcgttctc ggtattggtc ttggcgttac cgacgacgta
     4921 gatcgtcgag gggtcgtaca tagctacctc cggatcgaaa cgagctgcct ccggggccgc
     4981 gtctccgcga cggccccgga gcgccgatta tagtatcgaa agcaccttgc cgacgaggag
     5041 gccgccgccg gtgccgacga tatagcccat gagcgccatg agcacgccga tcggaaccag
     5101 ggcctcgccg taggacgccg ccaggatagg cgccgacgcg acgccgccga tattggcgag
     5161 gctcgcgacg ccgcaggtga acaggtcgag cttgcacagc ttggctacca gggcgagcag
     5221 gatggcgtgg atgcccagga tgcagaaacc ggccaggatg tagatcggag cctgggtcag
     5281 ctcggcgaag ttcgcacggg aggcgatcag gccgacgatg gtgtacagca tcaggttgga
     5341 caggtggttg gagccgggca gcttggccag cggggtcatc gcgcagatga cgccggcggc
     5401 ggtcgcgatc agcacggtcc aggtcgaact ctggaagaat acagacttgg gaagcatgcc
     5461 ccccagccaa atggacaggg cggagacgag gagactcgtg ccgagcagta cgatcaggtc
     5521 gacgaattcg acgttggtcc tgttgccctc ggtggcggcg ccgagcttct ggccgacctc
     5581 atcgaggacc ttggaatttg ccttggtcca cttgttgaac gccggagcaa acgaaacgac
     5641 ccagagcagg aacatcaccc agatcgaata gttgatcgag tcgatcagga gcgtgtagcc
     5701 catgttcgcg tcgggtacgt cgagagcgcc ctggatggcg accatgttgc cggtgccgcc
     5761 gatccagcta ccggacagcg ccgcgaaggc cttccagcta ccgggctcga aggcgccctt
     5821 gaagatggcg aaggtgacga tgaaacctat catgatggtg atcgtcgcgg cgaagaagcc
     5881 gaggagcatc ctcgggccga gcttgaagat cttgcgtaga tcgcagcgta gcagcatgag
     5941 gaagatcatc gccggcagca gcccatcctt gagggcctta tatgcggcgg tgatctgcgg
     6001 cgtcttctgc cagacgccga gcgtcgacag cagcatcgcg aagaaataga gcatgacgat
     6061 ggccggtacg tacttgaaga ccttgagctt cagggtcttc tcgaggaaga cgaggaggcc
     6121 tgcgaagaag atgaggaagg cgacataggg tagcgcgttc tggatcatag ggtttctctc
     6181 ctgttcgttg gtgtgcggcg ttgatcgtcg agacgacgat ggttcatcgg ggggacggcc
     6241 tccttcgcgc gcggagcgct gagccgccgg ccggagggag aggggcgaag cctcgagcgg
     6301 agacaggggc atcgcggact ccgtaggcgg acggctagaa taatacggcc ttaagccgtt
     6361 tacctagctt cgtttacgga cgatgcctga aggacaccca agcaaaccaa gcagtaaacc
     6421 gcgcgagaga gggcgcggca aggcgagtat acggtcgatt ctcggggcgc gcaaccgttt
     6481 tcgtttcgcg tcccgacgac ggtgcccgac tagtacctgg cgctcaggag cggaacgtac
     6541 ttggcccgca cggttgcgtc gtcgagcacg gcc
//
LOCUS       DAOOLN010000007         5887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_16221_length_5887_cov_3.248800, whole genome shotgun sequence.
ACCESSION   DAOOLN010000007 DAOOLN010000000
VERSION     DAOOLN010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16221_length_5887_cov_3.248800"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="PLW80_00195"
     CDS             <1..1174
                     /locus_tag="PLW80_00195"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB64947.1"
                     /translation="DPGRWGGGALELTRGCPYRCAFCFESKGEARLRRFPLETAGREL
                     ERFEAAMVEEVFVLDPTFNADPKRMAAAVRSFSERGPNLRYFLEIRAELLSAEQARLL
                     GSIDCSVQIGLQSADPAVLALVDRTLDPALFARKTRLLDEEGVVYGLDLIYGLPGDTL
                     AGFRRSLDYALGLGPNHLDVFRLAVLPGTALHDRASGLGLDWDRSAPYLVRSTPSFGD
                     AELDRAERLAVATDVLYTRGRAVMWFRSVAALAKAKPSALLAAFADFMDQERSGPLAG
                     IIAEARADAARHADIELLQTAFFGDWFKRRPTRKPDHALAAVDLIRASGAWTRAFAEG
                     EPTTLELGWPPEDLLDYASFDVAEFADETSRSPRRWTCEPGRDGPRFAPAKVKPGR"
     gene            complement(1171..2154)
                     /locus_tag="PLW80_00200"
     CDS             complement(1171..2154)
                     /locus_tag="PLW80_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPB64948.1"
                     /translation="MNPVVAAIADTPLFAGMSGLELNAVSAFLEPRRYAAGAVIFRRG
                     EPGTEMFIVRAGRVASLALDRDGNERQLYEFGPGRFFGEMAIIENEPRSATCVALETT
                     ELLVLEGIDFYRLVWEHPMIGSRMLASMAKVMAGWLDEASGFLTDLVRWGETARKRAV
                     TDELSGLFNRRFIEEASRSRFARNSSEPPLCSALMLDLDRFHALNERFGTMAGDSIIS
                     TAGAAYSRLIRDGDVAARLAGDEFAFFMPDAGPEEALAAAERIRSETESLYLEFRSGP
                     GATPQRVSLSASIGVASSPFDATDSAGLFAAADKALFSAKEAGRNRVVRFR"
     gene            complement(2151..3176)
                     /locus_tag="PLW80_00205"
     CDS             complement(2151..3176)
                     /locus_tag="PLW80_00205"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB64949.1"
                     /translation="MDGKEYGLADIVPLLKRSDFFSRLLPDDMYWLASKSELVARPSG
                     AVLFRPGDRAVRFFIVKSGSVAVSRLDAGGRAEEMARFVAGDVVGDFDFSRSAAYDAA
                     AVCAEDSEILSFPGRGATMDDLARERPDVSARILLRTVAMISSRVRSTQALISDNAPW
                     VRELRRQMYTDSATGLWSRSFLDDELARSIEPPAAVLLCKPDRFKELCDGWTHAAGDA
                     AMERIAALLKDEARSLRRAWAVRMRSNETAVIAAGYGLEQAAALAERIRAAYAAMDLS
                     PITGDSGFHLSASVAIAVWPDDGTDFKELVEQAYGLMTRAWKDGGDRVYRLGSFEPSP
                     GGPEGRR"
     gene            3634..4344
                     /locus_tag="PLW80_00210"
     CDS             3634..4344
                     /locus_tag="PLW80_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64950.1"
                     /translation="MYAPIGTVASGGIGLNFNAGFNIGRLFHEKLVLSPFAGVTPCFL
                     NAIKPGFIRDLADNYETPERLAYLQEHQDIEEERAYLDATSLLDYLMAGEALEKFSIT
                     MRYGVLVQLPWRFMPPVALYGLYSGTAIVGSETRTGYDTYTSGASGLSAYGYGMELYI
                     FRGPVWLNDGDITGSFHASSLSLIVEVTDLRNAIVRAADDYYGTPDIYLHEFTSDAFE
                     AAHPYDWRISINYNIHVF"
     gene            4356..5075
                     /locus_tag="PLW80_00215"
     CDS             4356..5075
                     /locus_tag="PLW80_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64951.1"
                     /translation="MKNDVQKLRSARIPIVGSVSRGIVAIAFLLSSCNLLFLPGMGFE
                     DKAPEFERLEVFDEDIESATLWPTFQATIQDDTGIASVSCSVLIDDGETKSLTIERAN
                     ERERYVWRVAGSLADVVSAGGNTITFTATDVADNSIEEIEYLSLDAGEGGLRFSIDST
                     QTPKVASFWHPSGITSVTYHYADDESNERTLDNTDDGTGDGGDRLFKGTLEAPPDDSY
                     NSAVFTITVNNSLSLTKLLYL"
     gene            5087..5845
                     /locus_tag="PLW80_00220"
     CDS             5087..5845
                     /locus_tag="PLW80_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64952.1"
                     /translation="MKPVALFTLAVLVLPLSAQDDEPDFAVSPLNLELGMQSVMLSPT
                     VAPAFGINMEFGLNPGWFINRRLGLGVFFAYAHYFGASHASSFLSAAADAYTPIEYMY
                     PGPGTTIDKMLEGSLDDLSDHRWGITIRPPIRYSPSLKLYWLSRAVVVSSGSSGQYSS
                     GGVTYVTGYGTTGYSYVGQGAGVELSAPLRVLEWGTWSVLQFTRVSLFAQYVNLRYAY
                     FDDPRPYFSEMFDEAFNDAYGDIVEIGLSVSLGF"
BASE COUNT         1039 a         1976 c         1863 g         1009 t
ORIGIN      
        1 cgaccccggg cgctggggcg gcggcgcgct cgagctgacg cgcggctgtc cctaccgctg
       61 cgccttctgc ttcgagtcga agggcgaggc gaggctcagg cgcttcccgt tggagacggc
      121 gggccgagag ctcgagcgct tcgaggcggc gatggtcgag gaggtcttcg tgctcgaccc
      181 gaccttcaac gccgacccga agcgcatggc ggccgccgtc aggtcgttct ccgagcgcgg
      241 tccgaaccta cggtacttcc tggaaatccg cgccgagcta ctaagcgcgg agcaggccag
      301 gctcctcggc tcgatcgact gttcggtgca gataggactc cagagcgccg acccggcggt
      361 gctggccctc gtcgaccgga cgctcgaccc ggccctgttc gcgcggaaga ccaggctact
      421 cgacgaggag ggcgtcgtct acggcctcga cctgatctac ggcctgcccg gcgacacgct
      481 cgccggcttc aggcgcagcc tcgactacgc cctcggcctc ggcccgaacc acctggacgt
      541 gttcaggctg gcggtactcc cgggcacggc cctccacgac cgcgcctcgg gcctcgggct
      601 cgactgggat cgctccgctc cctacctcgt gcgctcgacg ccgagcttcg gcgacgccga
      661 gctcgaccgg gccgagcggc tcgccgtcgc gaccgacgtc ctctacacgc gcggccgagc
      721 cgtgatgtgg ttccgctcgg tcgcggcgct ggccaaggcc aagccgtccg ccctgctcgc
      781 cgccttcgcc gacttcatgg accaggaacg atccgggccg ctcgccggca tcatcgcgga
      841 ggcgagggcc gacgcggcgc gccacgccga catcgagctg ctccagaccg cgttcttcgg
      901 cgactggttc aaacgcaggc cgacgaggaa gcccgaccac gcgctcgccg ccgtcgacct
      961 gatccgcgcc tccggcgcct ggacgagggc cttcgccgag ggcgaaccga cgacgctgga
     1021 gctcggctgg ccgccggagg atctactcga ctatgccagc ttcgacgtcg cagaattcgc
     1081 cgacgagacg agtcgttcgc cgcggcgctg gacctgcgaa cccggccgag acgggccgcg
     1141 cttcgcgccg gccaaggtca agcccggccg ctagcggaag cgcacgacgc ggttgcggcc
     1201 cgcttccttg gcgctgaaca gggccttgtc ggcggcggcg aacaggccgg ccgaatcggt
     1261 cgcgtcgaag gggctcgagg ccacgccgat gctggcgctc aggctgacgc gctggggggt
     1321 agcccccggg ccggagcgga actccaggta cagcgactcg gtctccgagc ggatgcgctc
     1381 ggcggcggcc agggcctcct cggggccggc atccggcatg aagaacgcga actcgtcgcc
     1441 cgccagtctc gcggccacgt caccgtcgcg tatcagccgg ctgtacgcgg ccccggcggt
     1501 cgagatgatc gaatcgccgg ccatcgtccc gaagcgctcg ttgagcgcgt ggaagcggtc
     1561 caggtcgagc atcagcgcgg agcatagcgg cggctccgaa gagttgcgcg cgaagcgcga
     1621 gcgcgaggcc tcctcgatga agcggcggtt gaacaggccg gatagctcgt cggtcaccgc
     1681 gcgcttcctg gccgtctcgc cccagcgcac cagatcggtc aggaagcccg aggcctcgtc
     1741 cagccagccg gccatcacct tggccatcga ggcgagcatc ctcgagccta tcatcggatg
     1801 ctcccagacg aggcggtaga aatcgatgcc ctcgagcacc aggagctcgg tcgtctcgag
     1861 cgccacgcag gtggccgagc gcggctcgtt ctcgatgatg gccatctcgc cgaagaagcg
     1921 gcccgggccg aattcgtaga gctggcgctc gttgccgtcg cggtcgaggg ccagcgacgc
     1981 gacgcgcccg gccctcacga tgaacatctc cgtgcccggc tcgccgcgcc tgaagatcac
     2041 ggcgcctgcc gcgtagcgcc tcggctccag gaaggcagag acggcgttca gctcgaggcc
     2101 gctcatgccg gcgaacagcg gggtgtcggc tatcgccgcg acgacggggt tcatcgacgc
     2161 ccctccggcc cgccgggact cggctcgaag ctaccgagcc ggtagaccct gtcgccgcca
     2221 tccttccagg cccgcgtcat cagcccgtag gcctgctcga cgagctcctt gaagtccgtt
     2281 ccgtcgtcgg gccagacggc tatcgcgacg ctggcgctca ggtgaaaacc ggagtccccg
     2341 gtaatggggc tcaggtccat cgcggcgtag gcggcgcgga tccgctcggc cagggccgcg
     2401 gcctgctcga gcccgtagcc ggcggctatc acggcggtct cgttgctgcg catcctgacg
     2461 gcccaagccc gcctgagcga tcgggcttcg tccttgagca gggccgcgat gcgttccatc
     2521 gccgcgtctc cggccgcgtg agtccagccg tcgcacagct ccttgaagcg gtccggcttg
     2581 cagagcagca cggcggccgg cggctcgatc gagcgggcca gctcgtcgtc gaggaaggag
     2641 cgcgaccaaa ggccggtcgc cgagtcggtg tacatctggc gcctgagctc gcgcacccag
     2701 ggcgcgttgt ccgatatgag cgcctgggtc gatcgcaccc gcgacgatat catcgctacg
     2761 gtgcggagga ggatgcgcgc cgacacgtcg ggacgctcgc gagccaggtc gtccatcgtc
     2821 gccccgcggc ccgggaacga caggatctcc gagtcctcgg cgcagacggc ggcggcgtcg
     2881 taggcggccg agcgcgagaa gtcgaagtcg ccgaccacgt cgccggccac gaagcgggcc
     2941 atctcctcgg cacggccgcc ggcgtccagc ctggacaccg cgaccgagcc cgacttgacg
     3001 atgaagaaac gcaccgcccg gtcgccgggc ctgaacagca cggcgccgga cggacgggcg
     3061 accagctccg acttggaggc cagccagtac atatcgtccg ggaggaggcg cgagaagaaa
     3121 tccgaacgct tgagcagcgg cacgatgtcg gccagcccgt actctttccc atccatgctc
     3181 cgtgaagtat aaacggcaat ccgccgtaat ggaatagaat cggagcgccc gggcgctctt
     3241 catcgtttcg agacaatgat aaagccccgg cagggttaat ccttccggtc atggagttgc
     3301 ctgtatgagt aagggttttc taggaaacgc cttttcgact tcgcttgaca aataaatata
     3361 gctacctaag ctacttgacg agaagcttga tttaaggaag cgaacttcag tcccgcccgt
     3421 ttccatagcc gctcgactga atttcgtcgc tggggtcgtg ccggagccat atacgaaacg
     3481 ttttgccaaa gccggagcat aaaaatgaaa cggacaatac tcgttgtagc gttactcgcg
     3541 gcggcgtcag cctcttcatt cgccgaagag tcggtactgg atttattttt ccccgaccat
     3601 acttcgagca tcgcctactt cgacgctggc gtcgtgtacg cgccgatcgg taccgtggcg
     3661 tcgggaggga tcgggctgaa cttcaacgcg gggttcaata tcggccgcct gttccacgag
     3721 aagctggtcc tatctccttt cgcgggcgtc accccctgtt ttctgaatgc catcaagccc
     3781 ggtttcattc gcgacctggc ggacaactac gagactcccg agaggctagc ctatctccaa
     3841 gagcatcagg acatcgagga ggagcgcgca tacctggatg ccacctcgct gctcgactat
     3901 ctcatggccg gcgaggctct ggagaaattt tccattacca tgcgctacgg cgtcctcgtg
     3961 cagctgccct ggaggttcat gccaccggtc gccctctatg gcctctatag cggaacggcc
     4021 atcgtgggaa gcgagacgag aacgggatac gacacgtata cctccggggc gagcgggctc
     4081 agcgcctatg gctatggaat ggaactatac atcttcagag ggccggtctg gttgaacgac
     4141 ggcgatatta ccggtagctt ccatgccagt tcgctctcgc tgatcgtcga ggtgaccgac
     4201 ctgaggaacg ccatcgtacg ggccgcggac gactactatg gtacgccaga catctacctc
     4261 cacgagttca cgtcggacgc attcgaagcg gcgcatccct atgactggcg gatttccatc
     4321 aactacaaca ttcacgtatt ttaggaacct gccgtatgaa gaatgatgta cagaaactga
     4381 gaagcgcacg catccccatc gtcggatcgg tatcgcgcgg tatcgtcgcc atcgcgttcc
     4441 tgctctcctc ctgcaatctg ctcttcctgc cgggcatggg cttcgaggat aaggcgccgg
     4501 agttcgagcg gctggaggta ttcgacgagg atatcgagag cgcgacgcta tggccgacgt
     4561 tccaggctac catacaggac gataccggta tcgcctcggt gtcctgctcc gtcctgatcg
     4621 atgacggcga gacgaagagc ctcacgatag agcgcgcgaa cgagcgtgag cgctacgtct
     4681 ggagagtcgc aggcagcctc gccgacgtcg tctccgcggg aggcaatacc atcaccttta
     4741 cggcgaccga cgtcgcggac aactcgatcg aagagataga gtatctctct ctcgatgccg
     4801 gcgaaggcgg cctcaggttc agcatcgatt cgacgcagac gccgaaggtg gcatcgttct
     4861 ggcatccgtc gggcatcact agcgttacat accactacgc agacgacgaa tcgaacgagc
     4921 gcacactgga caataccgac gatggcacgg gcgatggcgg cgacaggctg ttcaagggaa
     4981 ccttggaagc gccgcccgat gattcgtata atagcgccgt gttcaccata acggtaaaca
     5041 actcgctatc gcttacaaaa ctactgtacc tatgaggcat ctcagcatga aaccagtggc
     5101 cctattcacc ctagccgtcc tggtgcttcc cttgtccgcc caggacgacg agccggactt
     5161 cgccgtgtcg ccgctcaacc tcgagctggg catgcagtcc gtcatgctgt cgccgacggt
     5221 cgccccggcc ttcggcatca acatggagtt cggcctcaat cccggatggt tcatcaaccg
     5281 gaggctaggc ctcggcgtgt tcttcgcgta cgcccactac ttcggggcgt cccacgcctc
     5341 gagcttccta tcggccgcgg ccgacgccta tacgcctatc gaatacatgt atccaggccc
     5401 tgggacgacg atcgacaaga tgctcgaggg ttccctcgac gacctgagcg accatcgctg
     5461 gggtatcacc atccgtccgc cgatccggta ctcgccctcc ctgaagctct attggctgag
     5521 cagggccgtc gtcgtgtcgt ccggctccag cggccagtat tcatccggcg gcgtaaccta
     5581 cgttaccggc tacgggacga cgggatacag ctatgtcggc cagggagcgg gcgtcgagct
     5641 ctccgccccg ctccgcgtcc tcgagtgggg tacgtggagc gtcctccagt tcacccgcgt
     5701 cagcctgttc gcccagtacg tgaacctacg gtacgcctac tttgacgacc ctcgcccgta
     5761 cttcagcgag atgttcgacg aagcgttcaa cgacgcgtac ggcgacatcg tcgagatagg
     5821 cctgtcggta tccctgggct tctaaaacat ccgccgcagc tcggccgtcc agtcaagcgt
     5881 agacgcc
//
LOCUS       DAOOLN010000008         5671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_17080_length_5671_cov_3.704594, whole genome shotgun sequence.
ACCESSION   DAOOLN010000008 DAOOLN010000000
VERSION     DAOOLN010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17080_length_5671_cov_3.704594"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..518
                     /gene="ispF"
                     /locus_tag="PLW80_00225"
     CDS             39..518
                     /gene="ispF"
                     /locus_tag="PLW80_00225"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPB64953.1"
                     /translation="MRVGQGWDLHRLGPGRPLMLGGVEVPSELGELGHSDGDALFHAI
                     VDAMLGAVAVGDIGRHFPPSDPRWKDAPSRLFVERAAEILAGSGWRVVNVDATVVLER
                     PRLAPLADDIRASVADALGLSVGAVSVKAKTREGLGDVGRSEAVEAQAIVLVERLEP"
     gene            complement(519..2519)
                     /locus_tag="PLW80_00230"
     CDS             complement(519..2519)
                     /locus_tag="PLW80_00230"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF007746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HPB64954.1"
                     /translation="MIQLDAVDDGFVISLNGRRALLHSARVPLLELGTAETSARQRGG
                     AYKFGERRLSVVKAKAWKLATARDDFIDVEFDGLARMAIREAGGALRISLSRYDRAFN
                     RFMIRLPATPGESVFGCGERSSRLDLKGSIAPLWSQERGVAKGRGLAPLLSKARAGGA
                     WHTTSLGQPSFVTSERSYVHVGTNARCVFDFRRPRLTALRCWAIPDELVIGFADDMPA
                     AVGALSAITGRQPALPPWTWDGVWLGVEGGMAELRRKLDATRRAGIPVGAVWVRDWSG
                     RVRAGSSSRPRWDWRYDRELYPDLPAEIAALASDGIRFIGYANPYLDVDGELYAEASR
                     KGFCVKRDDGSDYQLAAQARPAAMVDLFNPEAFSWIKSVLIRELLGIGMAGWMADAGE
                     HLPVDAALHGDRDPLLAHNEYPALWARACAEAIVEAGKGAEATFFCRSGWAGSSRHAG
                     AFWAGDRTAGWSRRGGLPSVVPASLSLGFSGAGIWHSDIGGDATTAWARRSDECLMRW
                     AETAAFTPIMRTHEGDRPETNAQPWSNPATLAHFARLSSVWSALAPYHAETLAEYASS
                     GLPPIRHSWLHYEDQPELRALANQYLYGRDLLVAPVLEPGAELSKAVLPSDHWVHLWT
                     SREFMGGEVTIEAPLGYPPVFYRAASPWASLFESLRRTARKL"
     gene            2657..3445
                     /locus_tag="PLW80_00235"
     CDS             2657..3445
                     /locus_tag="PLW80_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64955.1"
                     /translation="MIHAALSSSAMIGADIATVLETASMAGVRGVEWSDDGFFDPRDP
                     SSVRAAMMATLRAGLCTVSYATLYRAVAHDRSAFRSALGVARDFNAPMLRLWSGPRGY
                     GGGLAARAFLDESRYLGDEAGKHGITLCFGISRRSVLASYDEATRVLAGTDHPFVKLA
                     WEPASRGGFDEAMEEFRPLSGRIGLIVVRGEDLLPEGGSADALSEEWYQYLDSYDEQC
                     GSPDMARYVVLSSTAGDQGRLAEAAGAIAAWSVMLRRYHRRRVM"
     gene            complement(3452..4288)
                     /gene="mazG"
                     /locus_tag="PLW80_00240"
     CDS             complement(3452..4288)
                     /gene="mazG"
                     /locus_tag="PLW80_00240"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPB64956.1"
                     /translation="MNTTTRNSAPEGAFRRLYDIFVRLRAPDGCPWDREQTPNSIRGN
                     LIEEAYECVEAINDGDLDHVREEIGDVMLVATMMAVIHEEQGDFTVAEALENVSDKLV
                     RRHPHVFADAEAETPDAVLRQWNDIKENVEGRRKKDSVLDSVSKALPPLERAYKLQKA
                     AAKVGFDWPDPTGPWDKLEEELRESREACAAIEAKAASGGDDSLERAALEDELGDLFF
                     SLVNVARKHRVDPAIAMHRALEKFSRRFRHVEKAMAAAGETMEPGKLALMDRYWDEAK
                     RA"
     gene            4636..>5671
                     /locus_tag="PLW80_00245"
     CDS             4636..>5671
                     /locus_tag="PLW80_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64957.1"
                     /translation="MKGSDVVIEHVRKSFGDFKALDDVSINIRKGEFFSLLGPSGCGK
                     TTLLRIIAGFESPEGGAVLLDGEDVVGQAPDARHVNTVFQNYALFPHLSVFENVAFSL
                     RLRRTPESEVKARVEEYLRLVELQAHASKKPAQLSGGQKQRVAIARALINEPSVLLLD
                     EPLSALDAKLRQHMLIELDSIHDKIGITFIYVTHDQQEALSVSDRIAVMNAGEVLQVG
                     TPQEIYEAPATDFVAKFIGETNLFEADVASVSGEMVELRTDGFGPVKVTATEPPPVGS
                     RVSWTIRPEKIRIGLEEPRSARNGINAVAGVVDEPIYSGFQSKFYVRTPSGLVLKVIK
                     QHADWSDEGSE"
BASE COUNT          887 a         1899 c         1952 g          933 t
ORIGIN      
        1 aatgtgaaga tcaccttccc ggaggacctc cggtgagcct gcgggtgggg cagggctggg
       61 acctgcaccg gctcggcccg ggcaggccgc tgatgctcgg cggtgtcgag gttccgtcgg
      121 agctaggcga gctcgggcat tccgacggcg acgcgttgtt ccacgccatc gtcgacgcga
      181 tgctcggcgc cgtagccgtg ggcgacatcg gccggcattt tcccccgtcg gaccctcgct
      241 ggaaggacgc gccctcccgc ctgttcgtgg agagggcggc cgagatcctc gccggctccg
      301 gctggcgcgt cgtcaacgtc gacgcgacgg tggtgctcga gcggccgcgg ctcgctcccc
      361 tggccgacga tatacgtgcc tccgtggccg acgcgctcgg cctgtcggtc ggggccgtgt
      421 cggtcaaggc caagacccgc gaggggctcg gcgacgtcgg ccgctccgag gctgtcgagg
      481 cccaggccat cgtcctggtc gagcggctgg aaccctagct acagtttccg cgcggttctt
      541 cgcaggctct cgaatagact ggcccagggc gacgccgccc ggtagaacac cggcggatag
      601 ccgagcggcg cctctatcgt cacctcgccg cccatgaatt ccctggacgt ccagagatgc
      661 acccaatggt cggacggcaa gacggccttg gatagctcgg cgccaggttc cagtaccggc
      721 gcgacgagca ggtcgcggcc gtagaggtac tggttggcca gggcgcgcag ctccggctga
      781 tcctcgtagt gcagccagct gtggcggatg ggcggcaagc ccgacgaggc gtattcggcc
      841 agcgtctccg cgtggtaggg ggccagcgcg gaccagaccg aggaaaggcg cgcgaaatgg
      901 gcgagcgtgg cggggttcga ccaaggctgg gcgttcgttt cgggtcggtc gccctcgtgc
      961 gtccgcatga tcggcgtgaa cgcggccgtc tccgcccatc gcatgaggca ttcgtccgag
     1021 cgcctcgccc aggccgtcgt cgcgtcgccg cctatgtcgg agtgccagat gccggcaccg
     1081 gagaagccga gcgatagcga cgccggcacg acgctcggca ggccgccgcg gcggctccag
     1141 ccggcggtcc ggtcgccggc ccagaaggct ccggcgtggc gcgatgatcc ggcccatcct
     1201 gagcggcaga agaaggtggc ctctgccccc ttgcccgcct cgactatcgc ctcggcgcag
     1261 gctctggccc agagggccgg gtattcgtta tgggcgagca gggggtcgcg gtcgccgtgg
     1321 agcgccgcgt ccaccggcag atgttcgccc gcgtcggcca tccaaccggc catgccgatg
     1381 ccgagcagct cccggatcag taccgacttg atccacgaga aggcctccgg gttgaatagg
     1441 tcgaccatcg cggccgggcg agcctgggcg gcgagctggt agtccgagcc gtcgtcgcgc
     1501 ttgacgcaga agcctttcct ggacgcctcg gcgtacagct cgccgtcgac gtccaggtat
     1561 gggttcgcgt acccgatgaa acggatcccg tcgctggcca gtgcggctat ctcggccggc
     1621 aggtcgggat acagctcgcg atcgtagcgc cagtcccagc ggggccgcga gctggagccg
     1681 gcgcggaccc ggccgctcca gtctcggacc cagacggcgc cgaccggaat accggcgcgt
     1741 cgcgtcgcat cgagctttcg ccgcaactcg gccatcccgc cctcgactcc gagccagacg
     1801 ccgtcccagg tccacggcgg gagcgctggc tggcggcccg tgatcgccga tagggcgccg
     1861 acggcggccg gcatatcgtc ggcgaagcct atgaccagct cgtcggggat tgcccagcaa
     1921 cgtagcgcgg tgagcctcgg acgcctgaaa tcgaagacgc agcgggcgtt cgtgccgacg
     1981 tggacatagg agcgctcgct ggtgacgaag gacggctggc cgaggctcgt ggtgtgccag
     2041 gcgccgccgg cccgggcctt cgacaggagc ggcgcgaggc ctcgtccctt ggccacgccg
     2101 cgttcctggg accatagcgg cgcgatcgag cctttgaggt ccagccgcga ggatcgctct
     2161 ccacagccga agacgctctc gcccggcgtc gccgggaggc gtatcatgaa gcggttaaag
     2221 gcgcggtcgt aacgggagag gcttatcctg agcgcgcccc cggcctcacg gatggccata
     2281 cgcgcgaggc catcgaattc gacgtctatg aaatcgtcgc gggcggtggc gagcttccat
     2341 gccttggcct tgacgacgga taggcggcgc tcgccgaact tataggcgcc gccgcgttgc
     2401 ctggccgagg tctccgccgt gccgagttcg agtaggggca cccgggccga gtggagcagg
     2461 gcgcggcggc cgttcaggct tatgacgaac ccgtcgtcga cggcgtcgag ctgtatcaat
     2521 ggaagacctc ccgccagacg gccggctcgg ccgaccgtat acggtaccat ataccgggtc
     2581 gatcgggccc gcaagaccga ttctggcggc gcaaggttgc catcagccat ggcttcaggt
     2641 agaatccgga cttaggatga tccatgcggc gctctcttcc tcggcgatga tcggggccga
     2701 tatagccacc gtactcgaga cggcctccat ggcgggcgta cggggcgtcg aatggtccga
     2761 cgacggcttc ttcgacccgc gcgacccgtc gtcggtgcgc gccgcgatga tggccacgtt
     2821 gcgggccggc ctctgtacgg tatcgtacgc caccctgtac cgcgccgtcg cccatgatcg
     2881 ctcggccttc cgctccgcac ttggcgtcgc ccgcgatttc aacgccccca tgctgcgcct
     2941 ctggtcgggg ccccgcggct acggcggtgg gctcgccgcg cgcgccttcc ttgacgagtc
     3001 gaggtacctg ggcgacgagg cgggcaagca cgggattacc ctatgcttcg gtatatcgag
     3061 gcgatcggta ctcgcctcgt acgacgaggc gacgagggtc ctggccggaa ccgaccatcc
     3121 cttcgtcaag ctggcctggg agcccgcgtc ccgcggcggc ttcgacgagg cgatggagga
     3181 gtttaggccg ctatccggac gaatcggcct catcgtcgtc cgcggcgagg atctcctgcc
     3241 cgagggcggc tcggccgacg cgctgtcgga ggaatggtac cagtacctcg actcgtacga
     3301 cgagcagtgc ggcagcccgg acatggcgcg ctacgtcgtg ctcagctcga cggcgggcga
     3361 ccagggacgc ctggccgagg ccgccggcgc gatcgccgcc tggagcgtca tgctcaggcg
     3421 ctatcaccgc cgtcgcgtga tgtagccgct atcaggcccg cttcgcctca tcccagtagc
     3481 ggtccatgag cgccaacttg cccggctcca tcgtctcgcc ggccgccgcc atcgccttct
     3541 ccacgtggcg gaaacgtctc gagaatttct ccagggcccg gtgcatcgct atcgcggggt
     3601 cgacgcggtg cttgcgcgcg acgttgacca gcgagaagaa caggtcgccg agctcgtcct
     3661 cgagcgcggc ccgctcaagg gagtcgtctc cgccggaagc ggccttggcc tcgatcgcgg
     3721 cgcaggcctc gcgcgactcg cgcagctcct cctcgagctt gtcccagggg cccgtcgggt
     3781 ccggccagtc gaagccgacc ttggctgccg ccttctgcaa cttgtaggcc cgctcaagcg
     3841 ggggcagggc cttggacacc gagtcgagga ccgagtcctt cttccggcgt ccctcgacgt
     3901 tctctttgat gtcgttccac tgcctgagca cggcgtccgg ggtctcggcc tcggcgtcgg
     3961 cgaacacgtg cgggtggcgg cggaccagct tgtccgaaac gttttcgagg gcctcggcca
     4021 cggtaaagtc gccctgctcc tcgtggatca ccgccatcat cgtcgcgacg agcatcacgt
     4081 cgccgatctc ttccctgacg tggtcgaggt cgccgtcgtt gatcgcctcg acgcactcgt
     4141 aggcttcctc tatcaggttg ccgcggatgc tgttcggggt ctgttcgcga tcccagggac
     4201 agccgtccgg ggcgcgcagg cgcacgaata tgtcgtagag gcgcctgaag gcgccctcgg
     4261 gggcggagtt tctcgtcgtc gtgttcatgg gggcattgtg gcgaggccgg gggggcttgc
     4321 gcaagggccg gggatggcgt tcgcgcctat cgcggatact ctagcccaag gcggatgctc
     4381 tcgccttagg cggataccgc gtgcatggcc gatgcataaa ccgcgcgaac cgcgccaaac
     4441 cgtgaataat gtaggcatac cgatggcata atcggcgttc aaaccgattg acatgctggc
     4501 ctaatatgcc gcattgtgcg cctgtctatg atcgattcag gcggatcgta ttccgccccg
     4561 ggccggtatc ggtatccgga aacggccgtc ccgtaaggcg gcatcatgga acgcggaggg
     4621 gaaaagcaaa agacggtgaa agggagcgac gtagtcatcg agcacgtacg caagtcgttc
     4681 ggcgatttca aggcgctgga cgacgtaagc atcaacatca ggaagggcga gttcttctcg
     4741 ctcttgggtc catccggctg cggcaagacg accttgctgc gcatcatcgc gggcttcgag
     4801 agccccgagg gcggagcggt gctgctcgac ggcgaggacg tggtaggcca agcgccggac
     4861 gcccggcacg tgaacaccgt cttccagaac tacgccctgt tcccgcactt atccgtgttc
     4921 gagaacgtgg ccttctcgct gcgcctccgt aggacgccgg agtcggaggt caaggccagg
     4981 gtcgaggagt acctgcggct cgtcgagctc caggcccacg cctccaagaa accggcccag
     5041 ctgtccgggg gccagaaaca gcgcgtggcg atagccaggg ctctcatcaa cgagccatcg
     5101 gtgctgctgc tcgacgagcc cctgtcggcc ctcgacgcca agctgcgcca gcacatgctg
     5161 atcgagctcg actccatcca cgacaagata ggcatcacct tcatctacgt tacgcacgac
     5221 cagcaggagg ccctgtccgt ctccgaccgc atcgccgtga tgaacgcggg cgaggtgctg
     5281 caggtcggca cgccccagga gatctacgag gcgcccgcca ccgacttcgt cgccaagttc
     5341 ataggcgaga ccaacctctt cgaggccgac gtcgcgtcgg tgtcgggaga gatggtcgag
     5401 ctccggaccg acggcttcgg cccggtcaag gtgacggcga ccgagccgcc cccggtcggc
     5461 tcccgcgtca gctggacgat acgcccggag aagatacgca tcggcctcga ggagcccagg
     5521 tcggccagga acggcatcaa cgccgtggcc ggcgtcgtcg acgagccgat ctactccggc
     5581 ttccagagca agttctacgt gcgcacgccg agcggtctcg tgctcaaggt gattaagcag
     5641 cacgccgact ggtccgacga ggggtcggag a
//
LOCUS       DAOOLN010000009         5428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_18097_length_5428_cov_3.031081, whole genome shotgun sequence.
ACCESSION   DAOOLN010000009 DAOOLN010000000
VERSION     DAOOLN010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18097_length_5428_cov_3.031081"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PLW80_00250"
     CDS             complement(<1..444)
                     /locus_tag="PLW80_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64958.1"
                     /translation="MNAALFAADLRLLWRHGFAIAYLVVAVLYAAALSALPRAWADAV
                     LPVLAWSDPTFFCFFFAGASVCLDLAQGTFRSLFASPLRPSAYLIVKALDLGILSFAM
                     AALVSASSRGAGFRLWPLAVSAVAGGAPTALLGATLALRLKSVNRF"
     gene            complement(441..1364)
                     /locus_tag="PLW80_00255"
     CDS             complement(441..1364)
                     /locus_tag="PLW80_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64959.1"
                     /translation="MIRVRDLRFTYPKGTSEAIRGIDFAVAPGEVFGFLGPSGAGKST
                     TQKILLGSLRGYSGSATVGSVESSGADSRYRAGIGAAFEFPCFYQKLTGLENIRLFAS
                     FYPGPKVDPRPLFERLGLADAMDRRLAEYSKGMRMRLNLIRAVIHRPRVLFLDEPTSG
                     LDPANARVVRDLVRERAADGAAVFLTTHDMAAADATCDRVAFIVDGRIAACDEPAALK
                     RRYGKRAVVVDREPGPEVGAAAGGSAAGASDGASDGASEKTAQAEYPLDGLADDAAFI
                     AELRKPGLLGVRTLDSSLDGIFLRLTGKELV"
     gene            complement(1361..2065)
                     /locus_tag="PLW80_00260"
     CDS             complement(1361..2065)
                     /locus_tag="PLW80_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB64960.1"
                     /translation="MPTAFDDRERAAIRERLLAAAVDALGRGGLGAASVAELAAAASI
                     AKGSFYLFYDSKEELFMDALEGIERDYRAIFEAAPALPGTAFERVHAAFQAAFAKVTS
                     DPAVTSMDGRTLERLTRALPPERVARHVEADAAAMATIAAAWSAAGLLGPGVGAAELA
                     GAGYAVFLMSTGLRNLPEGPRAATVAVAARGLALALAGAEGAVGEGSDERGGRRDSDT
                     LRRDSDTRRHEERRTR"
     gene            2227..3366
                     /locus_tag="PLW80_00265"
     CDS             2227..3366
                     /locus_tag="PLW80_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPB64961.1"
                     /translation="MPISFFKPFALERFFAKHEFSAKYLLCSSDCESFPVRELLAMDE
                     GSIDRLLDLRLGYTETRGAPSLREAIAGHYAGLGPEGVFVHSGAEEAILNLYLAALKA
                     GDRVVVNYPCYQSLAELPRSLGCEVIPWELRQVDDGRGRSRWRLDPDELPALSGGKAT
                     MIVLNAPHNPTGALPTRDEFEAIVSYARKTGAILFVDEVYRRLERDRAARLPSVCEAY
                     ENGVALDVLSKHSGLAGLRIGWLASRRVDLLDAVAVVKDYNSICSSAPSELFAEIAVR
                     NLEPIAARNRAICSRNLGLLDGFFERRQDFARWTPPAAGSVAFPRLSDGSDAEELAER
                     LVAESGVLLLPGAYYDYDRSYFRLGFGRANLPEALDRFELWLERR"
     gene            complement(3363..4598)
                     /locus_tag="PLW80_00270"
     CDS             complement(3363..4598)
                     /locus_tag="PLW80_00270"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB64962.1"
                     /translation="MRLLYDVHCHAMSLARPAIGRFVDAFLRGGLQSFFSQVAAPGYI
                     LAALGRNMGESVRNLFAVVENDPDALLELIENDLSGLYLSDDEAHRSVILPGGPKLLG
                     EDWDGWLVCPLLMDFGARATSHDTYYCEPIRKSLAEGVREALAGVSGYRRERPNGRLV
                     IRPFLGLDPGARGASETEAALSTYFSGFSRALPAQLRAFKSAARWKGDPASPPRNSFA
                     GVKLYPPLGFDPWPEDQAALDATRLLYAFCERRGVPIVVHCDDQGYRTIALDLAMRYT
                     DPERWVSALKAYPDLVVDFAHFGERYLGRSRARGAWTESIIRLMERYPGVYADTAFNG
                     CEPAYWDRLDRFVASLPAATSEIVRSRLLFGTDFVISLTKTRSYLDYVTDFASSSLDD
                     GFKRAMMTVNPAAFLFGAS"
     gene            complement(4615..5352)
                     /locus_tag="PLW80_00275"
     CDS             complement(4615..5352)
                     /locus_tag="PLW80_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64963.1"
                     /translation="MDAAWEPEVGSRIAAESPGIGLYAERGLHAALKTRYAARPSARV
                     EARVAGKVVDVVLPDELVEVQTRNLGSIAEKILHLACVMPVRLVHPIVVENLIVRVDP
                     ADGSAGAPRRSRTRRDFYSLFDELVRAPLLVASPNVRLEVVLVRVRELRVRDGTGSWR
                     RRGDRVLSRDLDEVLGVTSLDGRDDWLAIIPPGLAQPYDSASLGDALGVAAARARKIL
                     YAYAKAGLLEEAGKSGNRKLYRVPLSP"
BASE COUNT          854 a         1843 c         1938 g          793 t
ORIGIN      
        1 gaaccggttg acgctcttga gcctgagcgc cagggtggcg ccaagcaggg ccgtcggcgc
       61 gcctccagcc acggccgaca cggcgagcgg ccatagcctg aagccagcgc cgcgcgacga
      121 cgccgacacg agagcggcca tcgcgaacga caggatcccg agatcgagcg ccttgacgat
      181 caggtaggcg gatggccgca gcggcgaggc gaacagggag cggaaggtgc cctgggccag
      241 gtccaggcag acggaggcgc cggcgaagaa gaagcagaag aaggtagggt cgctccaggc
      301 taataccggg aggactgcgt cggcccaggc gcgcggcagg gccgacagcg cggccgcgta
      361 caggaccgcc acgaccaggt aggctatcgc gaagccgtgg cgccacaaga ggcgcaggtc
      421 ggcggcgaac agcgcggcgt tcatacgagc tcctttccgg tcagcctgag aaagatgccg
      481 tccaggctgg agtcgagggt gcgcacgccg agcaggcccg gcttccgtag ctccgcgatg
      541 aaggccgcgt cgtcggccag gccgtcgaga gggtactcgg cttgggcggt cttctcgctg
      601 gcgccgtccg aggcgccgtc cgaggcgcca gccgcggaac cgccggccgc ggctcccacc
      661 tccggcccgg gctcgcggtc gacgacgacg gcccgcttgc cgtagcgccg cttgagggcg
      721 gcgggctcat cgcaggcggc gatgcggccg tccacgatga aggcgacgcg gtcgcaggtg
      781 gcgtcggcgg cggccatgtc gtgggtggtc aggaataccg cggcgccgtc cgcggcgcgc
      841 tcgcgcacga ggtcgcgcac gacgcgcgcg ttggccgggt ccaggccgct cgtcggctcg
      901 tccaggaaca ggacgcgcgg gcggtggatg acggcccgga tcaggttgag ccgcatgcgc
      961 atacccttgg agtactccgc taagcgcctg tccatcgcgt cggccaggcc gagccgctcg
     1021 aacagcgggc gcgggtccac cttgggcccc gggtagaacg acgcgaacag ccgtatgttc
     1081 tcgaggccgg tcagtttctg gtagaagcag gggaactcga acgccgcgcc gatgccggcc
     1141 cggtagcggg aatcggcccc ggacgactcg acgctcccga ctgtcgccga gcccgagtag
     1201 ccgcgcagcg acccgagcag gattttctgc gtggtgctct tgccggcgcc gctcggcccg
     1261 aggaaaccga acacctcgcc gggcgccacg gcgaagtcga tgcctcgtat cgcctcggac
     1321 gtacccttcg gataggtaaa gcgcaggtct cgaacccgta tcatctcgtc cttctttctt
     1381 cgtggcggcg cgtatcggaa tcgcggcgga gcgtatcgga atcgcggcgg ccgcctcgtt
     1441 cgtcggagcc ttcacctacg gcgccttccg ctccggccag ggccaaggcc aggcctcggg
     1501 cggccaccgc gaccgtcgcg gcgcgcggcc cctcgggcag gttgcgcagg cccgtcgaca
     1561 tcaggaacac cgcgtagccg gcgccggcca gctcggccgc cccgacgccc ggcccgagca
     1621 ggcccgcggc cgaccaggcg gccgctatcg tcgccatcgc ggcagcgtcg gcctcgacgt
     1681 ggcgggccac ccgctcgggc ggcagggcgc gggtcagccg ctcgagcgtg cggccgtcca
     1741 tcgaagtcac cgccgggtcg ctcgtcacct tggcgaaggc cgcctggaag gcggcgtgta
     1801 ctcgttcgaa ggccgttccg ggcaaggccg gcgcggcctc gaaaatggcc cgatagtcgc
     1861 gctcgatgcc ttcgagcgcg tccatgaaca gctcctcctt cgagtcgtag aacaggtaga
     1921 agctaccctt ggctatggag gccgccgccg ccagttccgc cacgctcgcc gccccgagcc
     1981 cgccgcgccc gagcgcgtcg acggccgccg ccagcagccg ttcccggatc gcggcgcgct
     2041 ccctatcgtc gaacgccgta ggcatcgata cgtccctctt tcgcgctatg accatttata
     2101 cttatatggt catacggtaa tcgccatcgt ggcccctgtc aagcctcgcc gggccgttcg
     2161 tcgcagggct ttcccgaagc cccggacgtg gtatagtagc cgcgggacgc cgagcgtcca
     2221 cggaggatgc ccataagctt cttcaagccc ttcgccctcg agcgcttctt cgccaagcac
     2281 gagttcagcg ccaagtacct gctctgttcg tcggactgcg agtccttccc ggtgcgcgag
     2341 ctcttggcga tggacgaggg ctcgattgac cggctactcg acctcaggct cggctacacc
     2401 gagaccaggg gggcgccgtc gctccgggag gcgatcgcgg gccactacgc ggggctcggg
     2461 ccggagggcg tcttcgtcca ctcgggcgcc gaggaggcca tcctcaacct gtacctggcc
     2521 gcgctgaaag ctggcgaccg ggtggtcgtc aattatccct gctaccagtc gctcgccgag
     2581 cttccccgct cgctcggctg cgaggtgatt ccctgggagc tacgccaggt cgacgatggc
     2641 cgtggcaggt cgcgttggcg gctcgacccg gacgagctac cagccctgtc gggcggcaag
     2701 gcgacgatga tcgtgctgaa cgcgccccat aaccccaccg gcgccttgcc gacccgcgac
     2761 gagttcgagg cgatcgtgtc gtacgcgcgt aagaccggcg cgatcctgtt cgtcgacgag
     2821 gtgtatcgca ggctggagcg cgaccgcgcg gccaggctac cgtcggtctg cgaggcctac
     2881 gagaacggcg tggccctgga cgtgctcagc aaacactcgg gcctcgccgg cctccgaata
     2941 ggctggctag cgtcgcgccg ggtcgacctg ctcgacgccg tcgcggtcgt caaggactac
     3001 aactcgatct gctcctcggc gccgagcgag ctgttcgcgg agatagcggt gcgcaacctg
     3061 gagcccatcg ccgctcgcaa ccgcgcgatc tgctcgagga acctgggcct gctcgacggc
     3121 ttcttcgagc ggcggcagga tttcgcccga tggacgccgc cagcggcggg ttccgtggcc
     3181 ttcccgaggc tgagcgacgg atcggacgcc gaggagctgg cggagcgtct ggtagccgaa
     3241 tccggggtgc tattgctgcc gggagcgtac tacgactacg accgctcgta cttcaggctg
     3301 ggcttcgggc gggccaacct gcccgaggcg ctcgatcggt tcgagctctg gctggagcgg
     3361 cgctagctgg ccccgaacag gaaggccgcc gggttcacgg tcatcatcgc ccgcttgaac
     3421 ccgtcgtcga gcgaggacga cgcgaagtcg gtcacgtaat cgaggtacga cctggtcttg
     3481 gtcaggctga tgacgaagtc ggtgccgaac agtagccgcg aacggacgat ctcggacgtg
     3541 gcggcgggca gggaagcgac gaagcggtcc agccgatccc agtacgcagg ctcgcaaccg
     3601 ttgaacgccg tgtcggcgta gacgccggga taccgttcca tcagcctgat tatggactcg
     3661 gtccaggcgc cccgggccct ggagcggccg agatagcgct caccgaagtg ggcgaagtcg
     3721 acgaccaggt cgggataggc cttcagagcc gacacccagc gctcggggtc ggtgtagcgc
     3781 atcgccaggt cgagcgcgat ggtgcggtag ccctgatcgt cgcagtggac gacgatcggc
     3841 acgccccgcc gctcgcagaa cgcgtacagg agcctggtgg cgtcgagggc ggcctgatcc
     3901 tcgggccacg ggtcgaagcc gagcggggga tacagcttga cgccggcgaa cgagttcctc
     3961 gggggactgg ccggatcgcc cttccagcgc gccgctgact tgaaggcgcg cagttgggcc
     4021 gggagcgcgc gcgagaagcc cgagaagtag gtagacagcg cggcctccgt ctccgaggct
     4081 ccgcgggcac cagggtcgag gcccaggaag ggccggatca ccagccggcc gttggggcgt
     4141 tcgcgccgat agccggagac gccggcgagg gcctcgcgca cgccctcggc caggctcttc
     4201 cgtataggtt cgcagtaata cgtatcgtgc gacgtcgccc tggcgccgaa atccatcagg
     4261 agcgggcaga cgagccagcc atcccagtct tcgccgagta gcttcgggcc gccggggagg
     4321 atcacgctcc gatgcgcctc gtcgtcggac aggtagaggc cggacaggtc gttctcgatc
     4381 agctcgagca gggcgtcggg atcgttctcg acgacggcga acaggttgcg taccgactcg
     4441 cccatgttac ggccaagggc ggccaggatg taacctggcg cggccacctg gctgaagaac
     4501 gactggaggc cgccgcgcag gaaggcgtcg acgaagcggc cgatcgcggg gcgcgcgagc
     4561 gacatcgcgt ggcagtgtac gtcgtacagc agtctcatgg ccgacctcct cgcgtcacgg
     4621 actcaacggc actcgatata gcttgcgatt cccgctcttc ccggcctcct cgaggaggcc
     4681 ggccttcgcg taggcgtaga gtatcttgcg cgcccgcgct gccgcgacgc cgagcgcgtc
     4741 tccgagcgac gccgaatcgt agggctgggc caggccgggc gggatgatgg ccagccagtc
     4801 gtcgcggccg tccaggctgg tcacgccgag cacctcgtcg aggtcgcggg acagcacgcg
     4861 gtcgcctcgg cggcgccagg aacccgtgcc gtcgcgcacg cgcagctccc gcacgcgcac
     4921 gagcaccacc tcgagccgca cgttcggcga ggcgacgagg agcggagcgc gcacgagctc
     4981 gtcgaacagg ctgtagaagt cgcgtctggt cctggagcgc ctcggcgcgc cggccgagcc
     5041 gtcggcgggg tcgacgcgca cgatgaggtt ctcgacgacg atcggatgga cgagacggac
     5101 gggcataacg caggccaggt ggagtatctt ctcggctatc gagccgaggt tgcgggtctg
     5161 cacctcgacg agctcgtcgg gcaggaccac gtcgacgacc ttgccggcta ccctggcctc
     5221 gaccctggcg ctcggccgcg cggcgtagcg ggttttgagc gcggcgtgga ggccgcgctc
     5281 ggcgtagagg ccgatgcccg gggattcggc ggcgatccgg ctacccacct cgggttccca
     5341 tgcggcgtcc atgggcgtca gtatacacgc gccgcggctt agggcacaat gcgggcgatg
     5401 cgcatcgacg agctgcccat attcgacc
//
LOCUS       DAOOLN010000010         5294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_18715_length_5294_cov_3.106127, whole genome shotgun sequence.
ACCESSION   DAOOLN010000010 DAOOLN010000000
VERSION     DAOOLN010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18715_length_5294_cov_3.106127"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..1021
                     /locus_tag="PLW80_00280"
     CDS             233..1021
                     /locus_tag="PLW80_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HPB64964.1"
                     /translation="MKQRVVKAICLSFVIVFILAATGAFADDETVNLESLVVQTFDEP
                     DSQPWFLIGSKSATKGYPKLAYAKSWPVALFGTTGGDKDLRSLGVAMLFDRKEYNWVD
                     VVPGSKSGEGEEATYKPIELPLPGRVKMLDMWIWSGNFDYYIEAYIRDYTGIVHVLPM
                     GDLGHVGWKNFRVNIPNNVPQSKKYLPKSENLTLVKFRIWTRPTEVVASPTKADAPIE
                     QKAVYFYFDQLKVLTDTFESLFDGDSLMDPKVIEETWGSSDSSK"
     gene            1031..1768
                     /locus_tag="PLW80_00285"
     CDS             1031..1768
                     /locus_tag="PLW80_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HPB64965.1"
                     /translation="MKKIIAIVLAFSALASVSAQGAVGEPDASSIGIDTAQQKLQVVT
                     VDKFESAGFWNVYIMPDEGVANGRLFTGGPAAKADEPKPEERYVGIDPKIADVQTYGA
                     RVDFFKRGFNTVYVLAQKPIPIEGITKTISVWVVGRNYNHTLKIIVSDFFNNQFELTM
                     GKLNFHGWKKMSVAIPPQNPDGRTGIIQRNYHYNSQMGLKVVGFKIECDPMEAFGTYY
                     VYFDDLRAVTDLFAEDSRDEDDMVDGW"
     gene            complement(1834..2634)
                     /locus_tag="PLW80_00290"
     CDS             complement(1834..2634)
                     /locus_tag="PLW80_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPB64966.1"
                     /translation="MLEVEATGARLGLTDTHAHLSYIAERLGRRAIDELAAAYADAGA
                     MVLDPGVDYDDYPVRKAAFGDLGFVRLAAGIWPDAESIGDAEARVAALEAHLRDPACV
                     AVGECGLDYHWMHGGPSEQAELFSAQVELAVRLGKPLIVHSRDAHRDTLAIVRPVASR
                     IPVIIHCFGYDEEAAREYAAAGCYVSFAGNLSYKKSGALRAACAAVPGERLLLETDSP
                     YMCPEPRRGKDATPLDIGRTYALAAALRGSSIDDLAELVARNAAALFG"
     gene            complement(2627..5236)
                     /gene="clpB"
                     /locus_tag="PLW80_00295"
     CDS             complement(2627..5236)
                     /gene="clpB"
                     /locus_tag="PLW80_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002680241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HPB64967.1"
                     /translation="MDYDRFTLKAQEAIQAAHASAQKADHSQIELEHLLIALLDSDGS
                     AVRPLLQRLGASVDELESRLEASLAKKPKVYGHASQVYLSPAAGKALAKAEAEAAALK
                     DDYVAGEHVLLAMLDDDGSVGPLLKEFGVTKTGALEALKSVRGNRSVKDQAAEEKYRV
                     LEKYCRDLTALAHREKLDPVIGRDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAIVEG
                     LARRIVSGDVPDSLKGKKLLALDLGSLVAGAKFRGEFEERLKAVIEEVQKSDGAVILF
                     IDELHTLVGAGAAEGAMDASNLLKPALARGELKCIGATTLDEYRKHIEKDAALERRFQ
                     PVLCPEPSVEDTIAILRGLKERYEVHHGVRIMDEALIAAATLSDRYITSRFLPDKAID
                     LVDEAASRIKMEIESQPTDLDQIERRILQLTIEKQALSRESDPASLERLAKLDAELTE
                     LTARRDAMRLRWKNEKDRIEVVRRLKEDIERLKAEEQRHERDGNLAKAAEIKYGSLLE
                     AQKRLDEAAGAAKDAAGADGARLLREEVSEEDIARVVSTWTGIPVSKMMSSEIQKYLE
                     LEKILEKRVVGQEKAIVAVSDAIRRNRAGLSDPNRPLGSFLFVGPTGVGKTELAKTLA
                     EFLFNDEKSLTRIDMSEYMEKHTVSRLIGAPPGYVGYDEGGQLTEAVRRRPYSVVLFD
                     EVEKAHPDVFNALLQLLDDGRLTDGQGRTVDFKNCIVIMTSNLGSELILEAKDMDELR
                     GRVAELLKTAFKPEFLNRIDETVIFERLKPEDMGGIVDIQLAALAKRLADRSLSVSMT
                     DAAKALVARIGYDPRYGARPLKRVMQAEIQNPLSRLVLKGELREGDAVVIDAVKGGAV
                     QDGSFAFKVRRRA"
BASE COUNT          910 a         1721 c         1700 g          963 t
ORIGIN      
        1 cagcgcccgg gcacgttgcc ggagcccccg gaccgtagca agccggccag ttcggtggaa
       61 agccgagact tcgaggcctc ggcgagctaa taagccggtc gaagtcggcc gataatcgta
      121 tataccgtgc gcgggcgacc gtatttgttt gacaaaggta tggcttaaaa atataattcc
      181 acccgcagat ggtttatcgt taagatggtg caaaacttaa ggagtagtcc tgatgaaaca
      241 gcgcgtcgta aaggcgatct gcctgtcctt cgtcatcgta ttcatactcg cggcgacggg
      301 ggccttcgcc gacgatgaaa ccgtaaacct ggaatcgctg gtcgtacaga ccttcgacga
      361 gccggatagc cagccctggt tcctgatcgg cagcaagtcc gccaccaagg gctatcccaa
      421 gctggcctac gccaagtcct ggccggtagc cctgttcggc actaccggag gcgacaaaga
      481 cctccgcagc ctcggcgtcg cgatgctgtt cgaccgcaag gaatacaact gggtcgacgt
      541 cgtccccggc tccaagtccg gcgagggaga agaggcgacc tataagccta tcgagttgcc
      601 gttgcccggc cgagtcaaga tgctcgacat gtggatctgg tccggcaact tcgactacta
      661 catcgaggcg tacatccgcg actacacggg catcgtgcac gtgctgccga tgggcgatct
      721 cggccacgtc ggttggaaga acttccgcgt caacatccca aacaacgtgc cgcagtccaa
      781 gaagtacctc cccaagagcg aaaacctcac cctcgtcaag ttcaggatct ggacgaggcc
      841 gaccgaggtg gtggcgtcgc cgaccaaggc cgatgccccg atcgagcaga aggccgtcta
      901 cttctacttc gaccagctca aggtcctcac ggataccttc gagtcgctgt tcgatggcga
      961 cagcctcatg gatcccaagg tcatcgagga gacctggggc agttccgatt cgtctaagta
     1021 aggggagacc atgaaaaaga taatcgccat cgtattggct ttttccgccc tcgcttccgt
     1081 ctcggcccag ggggccgtcg gcgagcccga cgccagctcc ataggcatcg atacggccca
     1141 gcagaaactc caggtggtca cggtggacaa gttcgagtcc gctggcttct ggaacgtgta
     1201 catcatgccc gacgagggcg tcgccaacgg caggctcttc acgggcggcc cggcagccaa
     1261 ggccgacgag cccaagcccg aggagcgcta cgtcggcatc gaccccaaga tcgccgacgt
     1321 gcagacctac ggcgccaggg tcgacttctt caagcgcggc ttcaacaccg tctacgtact
     1381 cgcccagaag cccatcccga tcgagggcat caccaagacc atctccgtct gggtcgtggg
     1441 cagaaactac aaccacacgc tcaagatcat cgtatcggac ttcttcaaca atcagttcga
     1501 gctgactatg ggcaagctca acttccacgg ctggaagaag atgtccgtgg ccatcccgcc
     1561 gcagaacccc gacggcagga ccggcatcat ccagcgaaat taccactaca acagccagat
     1621 gggcctcaag gtggtgggct tcaagatcga gtgcgacccg atggaagcct tcggcaccta
     1681 ctacgtctac ttcgacgacc tgagggccgt caccgacctc ttcgccgagg actcccgcga
     1741 tgaggacgac atggtcgacg gctggtaata gtcgcttccc ataaaaaaag aagaccccgg
     1801 cgacggggtc ttcttttttt atggtctcgc gcctcagccg aagagagccg cggcgttccg
     1861 agccacgagc tcagccagat cgtcgatgct gctgccgcgt agcgcggcgg ccagggcgta
     1921 ggttctgcct atgtcgagcg gagtcgcgtc cttgccgcgg cgtggttcgg ggcacatgta
     1981 cggggaatcg gtctcgagga ggaggcgctc gcccggcacg gcggcgcagg ccgccctgag
     2041 cgcgcccgac ttcttgtagc tcaggttacc cgcgaaggat acgtagcagc cggccgccgc
     2101 gtactcccgc gccgcttcct cgtcgtagcc gaagcagtgg atgataaccg gtatccgcga
     2161 ggccacgggc cgcacgatcg ccaaggtgtc gcggtgcgcg tccctcgagt gtacgatcag
     2221 tggcttgcca aggcgtacgg ccaactcgac ctgagcggag aacagctcgg cctgctcgga
     2281 cggtccgccg tgcatccagt ggtagtcaag cccgcactcc cctaccgcga cgcaggccgg
     2341 gtcacgcagg tgcgcctcga gcgcggcgac ccgggcctcg gcgtcgccga ttgactcggc
     2401 gtccggccag atgcccgcgg ccaggcgcac gaagcccaag tcgccgaaag cggccttcct
     2461 gaccggatag tcgtcgtagt cgacgccagg gtctagtacc atggcgcccg cgtcagcgta
     2521 cgcggcggcc agttcgtcga tagcccgccg accgagccgc tcggcgatgt agctcagatg
     2581 ggcgtgcgta tcggtcaggc cgagccgcgc gcccgtagcc tccacttcaa gcacggcggc
     2641 gcaccttgaa cgcgaacgag ccgtcctgga cggcgccgcc cttaacggcg tcgatgacca
     2701 ccgcatcccc ctcgcgcagc tcgcccttga gcacgagccg agacagcggg ttctggatct
     2761 ccgcctgcat cacgcgcttg agcgggcgcg cgccgtagcg cggatcgtag ccaatcctcg
     2821 cgaccagagc cttcgccgcg tcggtcatcg atactgacag ggaccggtcg gccaggcgtt
     2881 tggccagcgc cgctagctgg atgtcgacga tgccgcccat atcctcgggc ttgagccgct
     2941 cgaagatgac cgtctcgtcg atgcggttga ggaactccgg cttgaacgcc gtcttaagga
     3001 gctcggccac gcggccgcgt agctcgtcca tgtccttggc ctcgaggatc agctctgagc
     3061 cgaggttgct cgtcatgatg acgatgcagt tcttgaagtc gacggtgcgg ccctggccgt
     3121 cggtcaggcg gccatcgtcg aggagctgca ggagggcgtt gaagacgtcc ggatgggcct
     3181 tttccacctc atcgaacaac accaccgagt acggccgtcg cctgaccgcc tcggtcagct
     3241 ggccgccctc gtcgtagcct acgtacccgg gcggcgcgcc tatgaggcgg ctgaccgtgt
     3301 gcttctccat gtactcgctc atgtcgatgc gcgtgagcga cttctcgtcg ttgaagagga
     3361 attcagccag ggtcttggcc agctcggtct tgccgacgcc cgtggggccg acgaagagga
     3421 agctgccgag cggccggttc gggtcggaca ggccggcgcg gttgcggcgt atggcgtcgg
     3481 acacggcgac gatggccttt tcctggccga cgacccgttt ctcgagtatc ttctcgagct
     3541 cgaggtactt ctggatctcg ctcgacatca tcttggacac gggtatgccg gtccaagtcg
     3601 acacgacgcg ggctatgtcc tcctcggaga cctcctcgcg gaggaggcgc gcgccgtcgg
     3661 ccccggccgc gtccttcgcg gctccggccg cctcgtcgag gcgtttctga gcctcgagca
     3721 ggctaccgta cttgatctcg gccgccttgg ccaggttccc gtcgcgttcg tggcgctgct
     3781 cctcggcctt gagccgctcg atgtcctcct tgagcctgcg gacgacctcg atgcggtcct
     3841 tctcgttctt ccagcgcagg cgcatcgcgt cgcgcctggc cgtcagctcg gtcagctcgg
     3901 cgtcgagctt ggccaggcgc tcgaggctgg ccgggtcgga ctcgcgcgac agggcctgct
     3961 tctcgatggt cagctggagt atgcgccgct cgatctggtc gaggtcggtt ggctggctct
     4021 cgatctccat cttgatgcgg ctcgccgcct cgtcgaccag gtcgatggcc ttatccggca
     4081 ggaagcggct ggtgatgtag cggtcggaca gcgtggctgc ggcgatgagc gcctcgtcca
     4141 tgatgcggac gccgtggtgc acctcgtagc gctccttgag accgcgcagt atggctatgg
     4201 tgtcctcgac cgacggctcc gggcagagca cgggctggaa gcgccgctcg agggccgcgt
     4261 ccttctcgat gtgcttgcgg tactcgtcca gggtggtggc gccgatgcac ttgagctcgc
     4321 cgcgggccag ggcgggtttg aggaggttcg acgcgtccat cgcgccctcc gccgcgccgg
     4381 cgccgaccag ggtatggagc tcgtcgatga acaggatgac ggcgccgtcc gacttctgca
     4441 cctcctcgat gacggccttg agccgttcct cgaactcgcc gcggaacttg gcgccggcga
     4501 cgagcgaacc caggtcgagg gcgaggagct tcttgccctt gagcgaatcg ggcacgtcgc
     4561 cggagacgat gcggcgggcc aggccctcga cgatggccgt cttgccgacg cccggctcgc
     4621 ctatgagcac cgggttgttc ttggtgcgcc tcgagaggac ctgcatcacg cggcgtatct
     4681 cctcgtcgcg gcctatcacc gggtcgagct tctcgcggtg ggccaaggcg gtcagatcgc
     4741 ggcagtactt ctcgaggacg cggtactttt cctcggcggc ctggtccttg acgctgcggt
     4801 tgccgcggac cgacttgagg gcctcgaggg caccggtctt ggtcacgccg aattccttaa
     4861 ggagcgggcc gaccgaaccg tcgtcgtcga gcatcgcgag caggacgtgc tcgcccgcca
     4921 cgtagtcgtc cttgagggcc gcggcctccg cctccgcctt ggccagggcc ttgccagcag
     4981 ccggtgacaa gtagacctgg gaggcatggc cgtagacctt gggcttcttg gccagcgagg
     5041 cctcgagcct cgactcgagc tcgtcgacgg aggcgccgag gcgctggagc agtggcctga
     5101 cggccgagcc gtcggagtcg agaagagcga tcagcagatg ctcaagctct atctgggaat
     5161 gatccgcttt ctgggcggag gcgtgggccg cctggatggc ctcctgggcc ttgagcgtga
     5221 acctatcgta atccatgggt gttctccgtt cgctgaacta gaacatacca agccacggct
     5281 ccggtcggca agcg
//
LOCUS       DAOOLN010000011         5149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_19420_length_5149_cov_4.022772, whole genome shotgun sequence.
ACCESSION   DAOOLN010000011 DAOOLN010000000
VERSION     DAOOLN010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19420_length_5149_cov_4.022772"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="PLW80_00300"
     CDS             <1..1020
                     /locus_tag="PLW80_00300"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HPB64968.1"
                     /translation="VRSGKSLGEANIGDDVLPDDMIKTAADGLVVIALDRSTGMRGTL
                     TIKSKSVAYIRLTPDAAGAKSTIEMVAGQLSSKLAKLSGNPTLQVQTDSVVMGVRGTQ
                     FSIAGSVNGSVLVVCTDGAVACTDGGEPVSVPAGKAVEKKAGERLKLVPVAISSAEQF
                     EKRWIADEIEAFKGNAVRALADYEKRYTDYMARFNEAFAPLQRSEVLSKWIREDAAGV
                     VPSAGDATTLREKKAIAGDILATRKVLFIFERLYYRIVELDQYVMGTTLEKAELRAGL
                     TVGDFVRRVRSDAPALEKRVFLFRYAEKLYEQRNSGGAGLPGMSAPGAGSEDDFFGSS
                     DDWDF"
     gene            1067..2266
                     /locus_tag="PLW80_00305"
     CDS             1067..2266
                     /locus_tag="PLW80_00305"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPB64969.1"
                     /translation="MTDYGTPFNYYGPTRLVFGRGVTAGAGALVAGLGVRSVLLVCGS
                     GATRRSAGFAALAASLEAAGVRATVFDKVTPDPHAALVAEAADAARSGSLEAVVAYGG
                     GSPIDCAKSAALAAANGGPILDYVRGKATPSRPALPIVAVTTTAGTGSEMSSAAVTTD
                     LASGQKLGYTNESFFPRIAIVDPENHTSMPTGVTAATGMDALTHAVESYLSLAANPLS
                     DAVNLRVVAMIARSLTRAYRDGADLEARSGMAIASAMAGVAFSQTGLGMVHGFAHPVG
                     ARHGIAHGQANAVILPYVMAALARDAAPRMRDLAAAMGACPSDADPPSGAASLVQAIV
                     DLKKGLGVPEGLSGVGISASDGPTLLTDAIGYRSRPRSPRAFSDAELGRLLDSAIAGD
                     LEAAAAL"
     gene            complement(2279..>5149)
                     /locus_tag="PLW80_00310"
     CDS             complement(2279..>5149)
                     /locus_tag="PLW80_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HPB64970.1"
                     /translation="RELVSHYAEPDDESFVELEFSVGRERWRVRRKPARTVPKKRGSG
                     LTDRPAEALLWRYGPTGWEPRSDRIDDIDAALARVIGLSAKEFTKIVLLPQGEFQRFL
                     EMRTGERTEILEKLFPVDLHDAVSELAKLKAKDAEAKARVLDDRAATMEAALGDDPGA
                     SLAEARSALAEARAADGAALAARDAANAASQLAAVAARAWDEYDAALAERAALEATEA
                     EAASLMARLSRAEAGLGAEAAVKAAGRARAEKAAAESSLAGAEATLAEADAETEGAEK
                     AGLLVAELAEALEAMDREAGLLSARAAAWQRLSVARARLESATAALSAAMAAKSTAET
                     AAAAASATLATIESGAAHAEALVAERNAAAIALESAREAERLAAEAAGFEAETRALEA
                     RTRAAAERSASAETVLASAVDTLAEAEDAVEALRDAQAAFRLASRLGPGSPCPVCGSV
                     EHPAPARPGTMAGGGRGGSSTDDGSGEANALRLEAARAALEAARAEAADAKAALSTLN
                     EAIVARAEGAARYAGALGPEAAEEGLRAARARLVAADRAQAAEAARGRAASAARVEYE
                     RLRKILDAAAASVSAAESERSAAAAAAAEASSGSGGDDPGPLLSSLRLRREAAASERA
                     KLESDIELWRRRRGEAVARASEAAARLERAVEALADTEAAVAIALAEAGFETEEAWAA
                     ACLPPPELADARERVRARAAAASSSEARFKAAERSVSGACRPDLAAAEAGRSAADEAY
                     AAARARLDAAAAAERELAAGLDALSAVRAERQAFRERGDRLVALSALLNGNAVGRHVS
                     FKIFTLASYFALVVERASARLREMSDGRYDMRVDDDRSAGRGFVGLDLSVLDSFTGVA
                     RPASSLSGGEKFLASISLALGLSDVIVERAGGVALDSIFIDEGFGSLDDETLDRAMEA
                     LDRVRGERVIGIVSHVAELRSRVPARVEVIKTNAGSSLRVVS"
BASE COUNT          727 a         1933 c         1789 g          700 t
ORIGIN      
        1 gtccgctccg gcaagtcgct cggcgaagcc aacatcggcg acgacgtgct gcccgacgat
       61 atgatcaaga ccgccgccga tgggcttgta gtcattgcgc tcgaccgtag cacgggcatg
      121 cgcggtacgc tgacgatcaa gtccaagtcg gtcgcttaca tccgcctgac ccccgacgca
      181 gctggcgcca agagcacgat agagatggtg gcgggtcagc tgagttccaa gctggccaag
      241 ctgtccggca acccgaccct gcaggttcag accgactcgg tggtgatggg tgtgcgcggc
      301 acccagttct cgatagccgg ctcggtcaac ggctcggtgc tcgtcgtctg taccgacggc
      361 gcggtcgcct gtaccgacgg cggcgagccg gtctccgtgc ccgccggcaa ggccgtcgag
      421 aagaaggccg gcgagcgcct caagctggta ccggtcgcga tctcgtcggc ggagcagttc
      481 gagaagcgct ggatagccga cgagatcgag gccttcaagg gcaacgccgt cagggccctg
      541 gccgactacg agaagcgcta taccgattac atggcccgtt tcaatgaggc cttcgcaccg
      601 ctccagcgca gcgaggtact gtccaagtgg atacgcgagg acgccgcggg cgtggtcccc
      661 tcggcaggcg acgcgacgac gctacgcgag aagaaggcca tagccggcga catcctggcc
      721 acgcgcaagg tcctcttcat cttcgagcgg ctatactata ggattgtgga actggatcaa
      781 tacgtgatgg gcacgacgct cgagaaggcg gagctcaggg ccggactcac ggtcggggac
      841 ttcgtccgcc gggtgcgttc cgacgctccg gccctcgaga agcgcgtatt cctgttccgc
      901 tacgccgaga agctgtacga gcagcgcaac tccggcggag ccggactacc gggcatgagc
      961 gcgccaggcg cgggctcgga agacgatttc ttcggctcgt ccgacgactg ggacttctag
     1021 gcggcccttc cccgccggcc cggttgcccg agccggcgga ggcgatatga ctgactatgg
     1081 cacacccttc aactactacg gcccaaccag gctggtcttc gggcgcggcg tcaccgccgg
     1141 cgccggcgcc ctcgtagccg ggctaggcgt acgctcggtc ctgctcgtct gcggctcggg
     1201 cgccacgcgc cggtccgcag gcttcgcggc tctggcggcc tcgctcgagg cggccggcgt
     1261 ccgcgcgacc gtgttcgaca aggtgacacc ggacccccac gcggctctgg tcgccgaggc
     1321 ggccgacgcc gcgcgctcgg gctcgctcga ggctgtcgtc gcctacggcg gcggtagccc
     1381 gatagactgc gccaagagcg cggccctggc ggcggccaac ggcggcccga tactcgacta
     1441 cgtgcgcggc aaggcgacgc catccaggcc cgccctgccc atcgtcgcgg tgacgaccac
     1501 cgccggcacc ggcagcgaaa tgagctcggc ggccgtcacc acggacctgg cgagcggcca
     1561 gaaactcggc tacaccaacg aatccttctt cccgcgcatc gccatcgtcg acccggagaa
     1621 ccacacgtcg atgccgaccg gtgtcaccgc ggcgaccggc atggacgccc tgacccacgc
     1681 ggtcgagagc tacctgtcgc tggcggccaa cccgctcagc gacgccgtaa acctgcgggt
     1741 cgtggcgatg atagcccggt cgctgacgag ggcctatcgc gacggggccg acctggaggc
     1801 tcgatccggc atggcgatag cgagcgccat ggccggcgtc gcgttctccc agacgggcct
     1861 gggcatggtc catggcttcg cgcacccggt gggcgccagg cacggaatag cccacggcca
     1921 ggccaacgcc gttatcctgc cctacgtgat ggccgcgctc gcgcgggacg cggcgccccg
     1981 gatgcgcgac ctggccgccg cgatgggcgc ctgcccgtcc gacgccgacc cgccctcggg
     2041 cgccgcgtcg ctcgtacagg ccatcgtcga cttaaaaaag ggactaggcg tgcccgaagg
     2101 cctgtcggga gtcgggataa gcgcttccga cggcccaacc ctgctaaccg acgcgatcgg
     2161 ctaccggagc cgccccagga gcccgagggc cttcagcgac gcggagctgg ggcgcctgct
     2221 cgattcagcg atagccggcg acctcgaagc ggccgcggct ctctgagccc gccccggctc
     2281 aggagacgac gcgcaggctc gagccggcgt tcgtcttgat cacctcgacg cgggcgggca
     2341 ccctggaacg cagctcggcc acatgagaga cgatacctat cacccgctcg ccgcgcacgc
     2401 ggtccagcgc ctccatagcg cggtcgagcg tctcgtcgtc tagggaaccg aagccctcgt
     2461 cgatgaagat cgagtcgaga gccacgccgc cggcccgctc gacaatcacg tcggacaggc
     2521 cgagcgccag cgatatcgac gccaggaact tctcgccgcc cgacaggctc gacgcgggcc
     2581 gcgccacgcc ggtgaaggag tcgagcaccg agaggtccag cccgacgaag ccgcgacctg
     2641 cggagcggtc gtcgtccacc cgcatgtcgt aacggccgtc ggacatctcg cgcaaccgcg
     2701 ccgacgcccg ctcgacgacc agcgcgaagt acgaagccag cgtgaagatc ttgaacgata
     2761 cgtggcgccc gaccgcgttg ccgttgagca gggccgacag ggcgacgagg cggtcgccgc
     2821 gctccctgaa cgcctgccgc tccgctcgga ccgccgacag cgcgtcaagt ccggcggcca
     2881 gctcgcgctc ggcggcggcg gcggcgtcca acctggccct ggcggccgcg taggcctcgt
     2941 cggcggccga ccgcccggcc tccgcagcgg ccaggtccgg ccggcatgcg cccgagaccg
     3001 aacgctccgc cgccttgaac cgggcctcgg acgaggacgc ggccgccgcc ctcgcccgta
     3061 cccgctcccg agcgtcggcg agttcgggcg gcggcagaca agccgcggcc caggcctcct
     3121 cggtctcgaa gcccgcctcg gcgagcgcga tcgcgacggc cgcttcggta tcggcgagcg
     3181 cctcgaccgc gcgctcgagc ctcgcggcgg cttccgacgc ccgcgccacg gcctcgccgc
     3241 ggcgccgccg ccacagctcg atgtcggact caagcttcgc tcgctccgag gccgcggcct
     3301 ctcgcctgag tcgcaaggac gagaggagcg gccccgggtc gtcgccgccg gagccgctcg
     3361 aggcctcggc cgctgcggcc gcggccgccg atcgctcgga ttcggccgcc gataccgagg
     3421 ccgccgccgc gtccaggatc ttcctgagcc gctcgtactc gacccgcgcg gctgaagcgg
     3481 cgcgcccacg cgccgcctcg gcggcctgag cccggtcggc cgccacgagc cgcgcccggg
     3541 ctgctcgcag gccctcctcg gccgcctccg gcccgagggc accggcgtag cgggccgcgc
     3601 cctccgctct cgcgactatg gcctcgttca gggtggacag cgcggccttg gcgtctgccg
     3661 cctcggccct ggccgcctca agcgcggcgc gggccgcctc gagccgcagc gcgtttgcct
     3721 cgccggaacc gtcgtcggtc gacgaccctc ctcttccacc gcccgccatc gtaccaggcc
     3781 tcgctggcgc cggatgttcg accgagccgc ataccggaca gggcgatcca ggtccgaggc
     3841 gggaggccag cctgaaggcg gcctgcgcgt cgcgtagcgc ctcgacggcg tcctcggcct
     3901 cggccagcgt atcgaccgcg gaggccagga cggtctcggc cgaggcggaa cgctcggccg
     3961 cagcccgagt ccgcgcctcg agcgcgcggg tctccgcctc gaagccagcg gcctcggccg
     4021 ccagccgctc tgcctcgcgg gccgactcga gcgctatggc ggcggcgtta cgctcggcta
     4081 ccagggcctc ggcgtgggcc gccccggact cgatcgtggc cagcgtggct gacgcggcgg
     4141 cggcggcggt ctcggcggtc gacttggcgg ccatggcggc gctcagggcg gccgtggccg
     4201 actcgagccg tgccctggcg accgataacc gctgccaggc ggccgcgcgg gccgacagga
     4261 gtcctgcctc gcggtccatc gcctcgagcg cctcggccag ctcggccacg agcaggccag
     4321 ccttctcggc gccctcagtc tcggcgtcgg cctcggccag ggtagcctcg gctccagcga
     4381 gagacgactc ggcggccgcc ttctcggccc gggcgcgccc ggccgccttc acggccgcct
     4441 cggcgccgag cccggcttcg gcccgcgaca acctcgccat cagcgacgcg gcctcggctt
     4501 cagtggcctc gagggcggcc cgctcggcga gcgcggcgtc gtattcgtcc caggccctgg
     4561 ccgcgacggc ggccagctgg gacgcggcgt tggcggcgtc gcgggcggcc agggccgcgc
     4621 cgtccgccgc cctggcttcg gcgagcgccg agcgcgcctc cgccaacgag gccccaggat
     4681 cgtcgccgag agcggcctcc atcgtagcgg cccggtcgtc gagcactctg gccttggcct
     4741 cggcgtcctt ggccttgagc ttggctagct cgctgacggc gtcgtgcaga tcgacgggga
     4801 acagcttctc gagtatctcg gtccgctcgc cggtcctcat ctcgaggaag cgctggaact
     4861 cgccctgggg cagcagcacg atcttggtga attccttggc cgacagcccg atcacgcggg
     4921 cgagcgcggc gtcgatatcg tctatgcggt cggatcgcgg ctcccagccg gtagggccgt
     4981 agcgccagag cagggcctcg gcaggccggt cggtcaggcc ggagccgcgc ttctttggca
     5041 ccgtcctggc cggcttgcgc cgtacgcgcc atcgctcccg cccgaccgag aactccagct
     5101 cgacgaagga ctcgtcgtcc ggctcggcgt agtgcgacac gagctcgcg
//
LOCUS       DAOOLN010000012         4801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_21330_length_4801_cov_2.719764, whole genome shotgun sequence.
ACCESSION   DAOOLN010000012 DAOOLN010000000
VERSION     DAOOLN010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21330_length_4801_cov_2.719764"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..437)
                     /locus_tag="PLW80_00315"
     CDS             complement(105..437)
                     /locus_tag="PLW80_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPB64971.1"
                     /translation="MPTYEYECRACGYTFERVQSMSDEPVRECPECGHEVRRLIFGGS
                     GVIFKGSGFYVNDSKGKNPAAPKAKPGASESAEPKAAEAKSGSDGVAPSKPSVPADPK
                     PAKAKESV"
     gene            814..1050
                     /locus_tag="PLW80_00320"
     CDS             814..1050
                     /locus_tag="PLW80_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64972.1"
                     /translation="MYVIFEQRERDAPGAAFVDGAWDSFFRELDRLLPTYRAATAQAR
                     RANSPARAAGRPDEEREEPTDDASGRSFQAPGAA"
     gene            complement(1047..2150)
                     /locus_tag="PLW80_00325"
     CDS             complement(1047..2150)
                     /locus_tag="PLW80_00325"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPB64973.1"
                     /translation="MPARKPLSRAPRKAKPSGKRVGVLGAGAWGTAVAKALAENGHDV
                     TIWAREGELAAAINERHENGAYLPGVALPERLRASTEPLDAANGSEALFIAVPSPYML
                     QTVKRVLTSPDIMEGRTLVAVLTKGFVATERGPRLIVETLEDYLPGFYRGNLVYVSGP
                     SHAEEVSRGLMTGLAAASVNGRNAIRVRELLQSQSLLVFPSLDVIGVQVCGAVKNVVA
                     IAFGMLDALKAESDAFGDNTESMLLAAGLNEIQAFGQALGATHPETFTSIAGVGDLDV
                     TCRSIHGRNRRFGREIVQKGVLAPFANAADLIARIGELGYLPEGAPASGFVDRLAAEK
                     RLSMPICRGVHRILDKELTPREFIEEYLGSLSG"
     gene            2220..3200
                     /locus_tag="PLW80_00330"
     CDS             2220..3200
                     /locus_tag="PLW80_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64974.1"
                     /translation="MNIEFHYYAVYALALEAGFDERTAFILAASSQEVDASTTPLAFD
                     APRGRVDLAVTQNYLFWDEAVKRDVYLPFHFVPGDQAKAAFARADGLSSAYTVTPNSD
                     NVKELLVAAFKDKDPYLMGIAAHAFADSWAHQNFCGLQDPTNDIVASLAASSLPPAGH
                     LQALSSPDEPDGVWHDRRLKAGSERVVNRERFAAAAIKLYRYLRVFLGKPFSDDELVV
                     ARLGAIWAKPSKDERLADYVICWNIRPYEHGLWRREAGSPPDASPFAGVRHYDKLAWA
                     KAQLSRATGTGQAAAVRTDSSFYRSDLYRWHEAALEHRKRATALLRKKGL"
     gene            3197..4090
                     /locus_tag="PLW80_00335"
     CDS             3197..4090
                     /locus_tag="PLW80_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64975.1"
                     /translation="MMPRTKRRLSRAASTLIAAFATLAAGSCASVVRAPADVEAERLL
                     PVGAAAYARLDRVALSAVLASMPGVDAKGASAIADRTDSMTLALVGAKDGSAAVVAVA
                     EGRYPAGAASMRLASDPSWRKDGQAWSTRDGRLHLAFASSGRAFVGTAPLDAVLAAAA
                     EPNPQPVPARWADEWAAPIAVYLPRPMDALRARLPLGDGAVPMSAMMLSARASSGQAA
                     SLGAYVASLAFEFDDERSAVIFAPICRLFLYVAATALWPERSATVLDAARWNASGRVV
                     RADGIPLDAEDLAAFVTLGAR"
     gene            complement(4280..>4801)
                     /locus_tag="PLW80_00340"
     CDS             complement(4280..>4801)
                     /locus_tag="PLW80_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64976.1"
                     /translation="DSGFCPEPVETYSYVSATSTEESGTVQIESSIVITSNATAFGDL
                     FYDAEMPNSATTCYGCLAAHPGEFSYGGSDSAFPEPIAGNEDLPNLALGGFFTAFSNA
                     YTGSYPFDYERWLGGSWRVSPGYSAYIGGGNLPYSGGSVAGFSVADYPALVLDLAGKA
                     RPTSGSWGRGAYE"
BASE COUNT          759 a         1680 c         1573 g          789 t
ORIGIN      
        1 acggaccgcc gagaagaagc ggccgcatac tgggcaggcc cgctcgtcgc gccccacctc
       61 cgtcccgcag tgttcgcaga agaagcgggg cgacttaccc gccctcagac gctttccttg
      121 gctttggcgg gcttcgggtc cgccggcacg ctaggcttgg agggagcgac accgtcggaa
      181 ccggacttgg cctctgcggc cttcggctcg gcggactcgg aggcgccggg cttggccttc
      241 ggggcggccg ggttcttgcc cttcgaatcg ttgacgtaga aacccgaccc cttgaagatg
      301 acccccgagc ctccgaagat cagcctgcgg acctcgtgtc cgcattcggg acactcgcgg
      361 accggctcgt cggacatgct ctggacccgc tcgaacgtgt aaccgcacgc tctgcattcg
      421 tattcgtacg tcggcatcgg aaacctcgtg gtactctctt gatgatgatt gagtgagcat
      481 aggataccga caaaggacgg ttcgagtaaa gtgtttctgt ccacttgccg cgcgcgccag
      541 tttctgagac tatgatgcgg gttcggacag gccggtcgga tctcagggcc gctcccgcgg
      601 ccgacgacgg caatccagga cctcggcgca cggaggcgct caatgatgat atcggtactc
      661 gacgccgcca aggcaggccg ctgaggggag tctccccgac ttccaccgct accgctcccc
      721 tccgcatcat ctcataggta tcagacggta aaaccggcgc tccggcgccg gaggatcgag
      781 cctagggccg atccagacgg aatggatccc atcatgtacg ttatattcga gcaacgcgag
      841 cgagacgcgc caggcgcggc cttcgtggac ggagcctggg acagcttctt ccgcgagctg
      901 gacaggctac tgcccacgta tcgcgcggcg acggcccagg cgcggcgcgc caattcgccg
      961 gcgcgggccg ctggacggcc cgacgaggaa cgggaggagc cgacggacga tgcgtccggc
     1021 cgctccttcc aagccccagg cgccgcctag cccgacaggc ttcccaggta ttcctctatg
     1081 aactcgcgcg gcgtcagctc cttgtcgagg atgcggtgca cgccgcggca aataggcatg
     1141 gacaggcgct tctcggcggc taaccggtcg acgaagccgc tcgccggcgc tccctcgggc
     1201 aagtagccga gttcgcctat cctggctatc aggtcggcgg cgttcgcgaa cggcgccagc
     1261 acgcccttct gaacgatctc gcggccgaag cgacggttgc gcccgtggat cgagcggcag
     1321 gtcacgtcga ggtcgccgac cccggctatc gaggtgaagg tctccgggtg cgtggcgccg
     1381 agagcctgcc cgaaggcctg gatctcgttg agcccggccg cgagcagcat cgactcggta
     1441 ttgtcgccga acgcatccga ctccgccttg agcgcgtcca gcatgccgaa ggctatcgcg
     1501 acgacgttct tgaccgcgcc gcagacctgc acgcctatca cgtcgagcga cgggaagacg
     1561 agcaggcttt ggctctgcag gagctcccgc acgcggatgg cgttgcgccc gttgacgctc
     1621 gccgccgcga ggccggtcat cagcccccgc gacacctctt cggcgtggct cggccccgag
     1681 acgtagacca ggttgccgcg gtagaagccg ggaaggtaat cctcgagcgt ctcgacgatc
     1741 agcctcggcc cgcgctcggt cgcgacgaag cccttggtca gtacggcgac gagggtacgc
     1801 ccctccatga tgtccggcga ggtgagcacc cgcttgaccg tctgcagcat gtacggcgac
     1861 ggcaccgcga tgaacagcgc ctcgctgccg ttcgcggcgt cgagcggctc ggtgctggcg
     1921 cgtagccgct ccggcagggc gacgccgggc aggtacgcgc cgttctcatg ccgctcgttg
     1981 atggcggccg ccagctcgcc ctcgcgggcc catatcgtca cgtcgtggcc gttctccgcc
     2041 agggccttgg ccaccgccgt gccccaggcc ccggcgccga gcacccccac ccgcttgccg
     2101 gacggcttgg ctttcctggg cgccctcgat agcggctttc gtgcaggcat atcgcctcct
     2161 cggtatagtc ccggaccgtg catagccgcg ggccgggcgt atgagtatct ttgcgatcga
     2221 tgaatataga attccattac tacgcggtct acgcgctcgc cctggaagcc ggcttcgacg
     2281 agcggaccgc cttcatcctg gccgcctcgt cgcaggaagt cgacgcgtcg acgaccccgt
     2341 tggctttcga cgcgccgcgc ggccgcgtcg acctggcggt gacccagaac tacctgttct
     2401 gggacgaggc ggtcaagcgc gacgtctacc tgcccttcca cttcgtgccg ggggatcagg
     2461 ccaaggccgc ctttgcccgg gcggacggct tatcgtcggc ctataccgtg acacccaact
     2521 cggataatgt caaggaactg ctcgtcgccg ccttcaagga taaggacccc tacctgatgg
     2581 gcatcgcggc gcacgccttc gccgacagct gggcccacca gaatttctgt ggcctccagg
     2641 acccgaccaa cgacatcgtc gcctcgctcg cggcctccag cctaccgccc gccggccatc
     2701 tccaggccct gtcgtcgccc gacgagcccg acggcgtatg gcatgaccgc aggctcaagg
     2761 ccggctcgga gcgggtcgtc aacagggagc gattcgccgc cgcggcgatc aagctctacc
     2821 gctacctccg ggtgttcctc ggcaagccct tctccgacga cgagctcgtc gtcgccaggc
     2881 tcggggcgat ctgggctaag ccgtccaagg acgagcgttt ggcggattac gtgatatgct
     2941 ggaacatacg gccctacgag cacgggctat ggcgccgcga ggcgggctcg ccacccgacg
     3001 cctcgccgtt cgccggcgtc aggcactacg acaagctggc ctgggccaag gcccagctgt
     3061 cgagggctac tggaaccggg caggccgcgg ccgtacggac ggattcgtcg ttctaccgga
     3121 gcgacctgta ccgctggcac gaggcggcgc tcgagcaccg gaagcgcgcg accgcgctcc
     3181 tgcgcaagaa aggactatga tgccgagaac gaagcggagg ctatcacgcg cggcctccac
     3241 cctcatcgcc gccttcgcga ccctggccgc cggctcctgc gcgagcgtcg tgcgagcccc
     3301 cgccgacgtc gaggccgagc gcctcctgcc ggtcggcgcc gcggcctacg ccaggctcga
     3361 ccgggtcgct ctatcggccg tgctcgcctc gatgcccggt gtggacgcca agggcgcgtc
     3421 ggccattgcc gaccgcaccg actcgatgac cctcgccctc gtcggcgcga aggacggctc
     3481 ggccgccgtc gtcgccgtgg cggagggccg ctaccccgca ggggccgctt cgatgcggct
     3541 ggcgtccgac ccgtcgtggc gcaaggacgg ccaggcctgg tcgacccgcg acggcaggct
     3601 ccacctggcc ttcgcctcgt cggggcgcgc cttcgtcggc accgcgccgc tcgacgcggt
     3661 gctggccgcg gccgcggagc cgaaccccca gccggtgccc gcgcgctggg ccgacgagtg
     3721 ggccgctccc atagccgtct atctgccgcg cccgatggac gccctgcgcg ccaggctgcc
     3781 gctcggcgac ggcgccgtgc cgatgagcgc catgatgctc tcggcccgcg cctcctccgg
     3841 ccaggccgcc tcgctcggcg cctacgtcgc gtcgctcgcg ttcgagttcg acgacgaacg
     3901 ctcggccgtg atcttcgcgc cgatctgcag gctgttcctg tacgtcgccg ccaccgccct
     3961 ctggcccgag cgttcggcga ccgtgctcga cgccgcccgg tggaacgcct ccggccgcgt
     4021 agtccgtgcc gacggcatcc ccctcgacgc ggaggacctg gcggccttcg tcacgctggg
     4081 cgcgagatag cagggtcgcg gaggcgctca gaacgcgatg aagatagcga ataggctaat
     4141 tcgaacatga tgacaggatg gaggaacgat gaacaaggta agaccctaag gttaatcgat
     4201 tcatcgtgtc catcgtgtcc ccatcatgag catagtgttc atagtcttag cctatacggt
     4261 aggagcgtct tggcgttcat tattcgtagg cgccgcggcc ccaggaaccg ctcgtcggcc
     4321 tggccttgcc cgccaggtcg aggaccagcg ccggatagtc ggctactgag aaacccgcca
     4381 ccgatccgcc tgaatacggc aggttaccgc cgccgatata ggctgagtac cccggcgaaa
     4441 cgcgccacga gccgcccagc caacgctcgt agtcgaacgg atagcttccc gtataggcgt
     4501 tcgaaaacgc cgtgaagaag ccgccgaggg ctaggttcgg gagatcctcg ttgcccgcga
     4561 taggttcggg gaacgcgctg tccgagccgc catacgagaa ttccccgggg tgcgccgcga
     4621 ggcaaccgta gcaggtggtc gcgctgttcg gcatttcggc gtcatagaac aggtcgccga
     4681 aggccgtggc gttcgaggtt atgacgatgc tcgactcaat ctgcaccgta ccgctttcct
     4741 cggtggaggt ggcggagacg tagctgtagg tctcgaccgg ttcggggcag aaccccgagt
     4801 c
//
LOCUS       DAOOLN010000013         4776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_21492_length_4776_cov_2.891548, whole genome shotgun sequence.
ACCESSION   DAOOLN010000013 DAOOLN010000000
VERSION     DAOOLN010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21492_length_4776_cov_2.891548"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            362..694
                     /locus_tag="PLW80_00345"
     CDS             362..694
                     /locus_tag="PLW80_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPB64977.1"
                     /translation="MKKADTTRILLACNMKRLREALGLSQMSLAERVGCSTTLIGNIE
                     TLKRFPSAENLDRIAVALEVPVYELFMKDSPAIERAVSKFEVRERLEQKVLKAIEEAL
                     DEDERADK"
     gene            1159..1488
                     /locus_tag="PLW80_00350"
     CDS             1159..1488
                     /locus_tag="PLW80_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPB64978.1"
                     /translation="MPKKLPILLPSVQRSLEALGENIRLARLRRDLSAEMVAERAGIS
                     RQTLSALENGSSSVSMATWIQVLFVLGLEKDLEAVARDDELGRALQDAKLPTRKRARK
                     SPQKDEE"
     gene            1485..2744
                     /locus_tag="PLW80_00355"
     CDS             1485..2744
                     /locus_tag="PLW80_00355"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HPB64979.1"
                     /translation="MSEAGRTIEVYADWIGLDSATRLGTLSVQAAQGKEVFSFEYDAT
                     WLRRDDNRILDPDLRFYKGPQYAGSGKPNFGLFLDSSPDRWGRVLMRRREAILARKEG
                     RAPRRLGEADYLLGVYDKNRMGALRFKVPGSEAYQSSDAAMATPPWSTLRDLEYASFL
                     VEHEEDDEKLDPWLAMLIAPGSSLGGARPKASVVDEKGELWIAKFPGKDDERDWGAWE
                     MLVADLARVAGIEMSECRLDSFSKRGSTFLTKRFDRIGEGRIHFASAMTLLGRKDGDD
                     AAAGASYLELAEFIMRSGARPDADLGELWKRIVFSIAVSNTDDHLRNHGFLLDAKGWR
                     LSPAYDINPSPDSAGLSLAIDEYDNSLDFELALSVAPYFRLSKPEALSIMESIRGVTA
                     TWRERAKALGIRKPEIERMQGAFENQH"
     gene            3817..4260
                     /locus_tag="PLW80_00360"
     CDS             3817..4260
                     /locus_tag="PLW80_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HPB64980.1"
                     /translation="MDQLLHMAQSVAVFRSMYIRHSRSEPGGGGSWRFLILIHNFDEG
                     LGIKPADMARYFRCSRAYVSKMIKELTADGLVEDRPDERDLRSRYLACTEKGVRLARG
                     IMDDYVAVTRRLRDGLGVRKSEQLVSLLDEAVAILERVNDGEDRE"
     gene            4351..>4776
                     /locus_tag="PLW80_00365"
     CDS             4351..>4776
                     /locus_tag="PLW80_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64981.1"
                     /translation="MSSFKNLRIVDNFYQTSMFYPMPTMLISTLSPSGATSLGAYSLC
                     FPYYIAGKEYYAMLLECRNSSNTAQNLLRTGVCAINFLEDSRRLFKETVRLGFPGERC
                     EEKMSDCFLEMEAGQAADKESDGGARPLVLKDAYQVLECS"
BASE COUNT         1017 a         1387 c         1393 g          979 t
ORIGIN      
        1 ccactccgcc gccacacgca cggtaggctc atcggagagc tggcatgagg ccagacgctt
       61 cagcatatcc tcatccaaga agtcccagtg ttccgccaag gcccggaggt actggacgag
      121 gaaggtgctc ccgaaaccgt gatcggcata gggatcgagg agatagttga atatcgaaga
      181 tacaaccgtc tcaccgaagg ccttgttcta ggtggcgagg actcgcaaga tgtccatgct
      241 ggctcctttg taatggtgtg gattcgatta tagcctaata gcctatacca gcaaaggctt
      301 atcggcgatt gaggcctccc cgtccaagag tgacactgac gtgcacggaa ggggcgaatc
      361 attgaagaaa gccgatacca cgaggatatt gctcgcctgc aacatgaagc gtctccgcga
      421 ggccctgggg ctctcccaga tgtccttggc cgagcgagtc ggctgctcga ccaccctcat
      481 aggcaacatc gaaaccctca agcgcttccc ttccgcggag aacctcgacc gcatcgccgt
      541 ggccctcgag gtgcccgtat acgaactctt catgaaggac tcgcccgcga tcgagcgcgc
      601 tgtctccaag ttcgaggtcc gggaacgcct cgagcagaag gtgctcaagg ccatcgaaga
      661 ggcgctggac gaggacgagc gggcggacaa gtaggcattg cggaggtatc cgctaggaca
      721 agcccaagtt ccagcgatcg cttcggacga gagcttgcaa gacgtgtata ggaaagaccg
      781 agaacctata caggtcgccg gctcatgtat agattcttca ccttttcttg cttcaggcgg
      841 taacaggcga ggacactgtt gccagggctc gcatgttccg aaatgtcggc acttgttatc
      901 aggtgtcgac acttcggaac accaggatgc cggagctggc ggctcatcga gcctctttct
      961 tcatccgtac taaaatcgcg ctttctttag tcggatttca tccacttcag tcctgcccag
     1021 cctgcaaatg gcacttcttc catggatgca ccaaaacgaa gaacaagtct ctgatccaaa
     1081 tttcaatgat ccttggtttg aggtttacat cgtaataatt tgacaagacg gctttttatg
     1141 ggtactatat tagacattat gcccaagaag ctcccgatcc tattgccaag cgtccagcgc
     1201 agtctcgaag cgctcggcga gaatatccgc ttagcccgcc ttaggcgtga cctcagcgcc
     1261 gagatggtcg ccgagcgggc cggcatcagc cgacagaccc tgagtgccct cgaaaatggc
     1321 tcgagctcgg tctcgatggc tacctggatt caggtcttgt tcgtgctagg gctcgaaaag
     1381 gatcttgagg ccgtcgcgcg ggatgacgaa ctcgggcgcg ccctccagga cgccaagctt
     1441 ccaactcgga agcgtgcccg gaaatcgccg cagaaggacg aagaatgagc gaggccggac
     1501 gcaccatcga ggtatatgcc gattggatcg gcctcgattc cgctacgcgc ctcgggacat
     1561 tgtccgtcca ggcggcccaa ggcaaggaag tattctcctt cgagtacgac gcgacctggc
     1621 ttcgacgcga cgacaatcgg atcctcgatc ccgatctgcg tttctacaaa ggcccccagt
     1681 atgccggctc ggggaagccc aatttcggtc tgttcctgga ttcctctccc gatcgttggg
     1741 ggcgtgtcct catgcgacgg cgcgaggcca ttctcgccag gaaggaaggg cgtgcgccgc
     1801 gaaggctcgg agaagcggac tacctgctcg gcgtctatga caagaaccgg atgggagcgc
     1861 ttcgcttcaa ggtaccgggc agcgaggctt atcaaagctc ggatgcggcc atggcgactc
     1921 caccctggtc gacgttgcgc gatctcgagt atgcgagctt cctcgtcgag catgaagagg
     1981 acgatgaaaa gctcgatccc tggctcgcca tgctgatcgc gcccggctct tcgctcggag
     2041 gcgcacgacc caaggccagc gtggtggacg agaagggcga gctctggata gccaagttcc
     2101 ccggcaagga cgatgaacgc gactggggcg cctgggagat gctcgtcgcc gacctcgccc
     2161 gcgttgctgg catcgagatg agcgaatgca ggctcgactc cttctcgaag cgaggctcga
     2221 ccttcctgac gaagcgcttc gatcgcatag gcgagggacg gatccacttc gcctccgcca
     2281 tgaccttgct gggccgcaag gacggcgatg atgccgcggc cggcgcgagc tacctcgagc
     2341 tcgctgagtt catcatgcgc tccggcgcac gccccgacgc cgacttgggc gagctctgga
     2401 aacgcatcgt cttctccatc gccgtctcaa ataccgacga tcatctccgg aatcacggct
     2461 tcctcctcga cgcgaagggc tggcgacttt cgcccgctta cgacatcaat ccctcgccgg
     2521 attcggcggg cctttccctc gccatcgacg agtacgacaa ctcgctggac ttcgagctgg
     2581 ccctgtcggt cgcgccgtac ttcaggctat cgaaaccgga ggctctctcg atcatggagt
     2641 cgatacgcgg tgtgacggct acgtggcggg agcgggcgaa agccttgggc atccggaagc
     2701 ccgagatcga gcggatgcag ggagccttcg aaaaccaaca ttgagaactc gcccaatgat
     2761 ggaccccgaa acccctgaag tgtaactaga agtgcgactg gaaatacccc cgaactgcga
     2821 ctaaattctg caatagcgtg cagaggttta caaaggctgc tagaataatt ccagttacag
     2881 tagatacttt acaaagctat gtcattgcag gataacgagt tatcctgttt cgcaatctat
     2941 aggtcagggg ttcgaatccc ctattctcca tcagtacttg cgaagttggt tgcctcctga
     3001 aagtacactg tgacttgccg gtgtgacttt tggggtttcc atttatgggc aagaagacca
     3061 agaccgaccc aagccgcttc cacttcacct tattcaagcg atccactcaa gacggcacgg
     3121 ttctcattta tgccaggctg ctcgagactt cgaccgggcg tattctcgct cagcggccca
     3181 cgggtacgga cgacgaacga ctggcggccg ccaaggcgga gcaacttctc gtcgagtttc
     3241 ccctagccaa gttggctcag ttgaaatcag ccagagatca ggagggtttc gaggcggcgg
     3301 aacgtctacg gaacatggat ctagccagct tcttcgtctg gttctggact cctagcgtct
     3361 cagaatatct atggatccga atcgacgccg agaagccttt ctcgaactac tatgtcctct
     3421 gccagtcccg ctcagtagcc cgagaagtca cgatggtgtc ggcagttacc actaaagtca
     3481 aagttaatta tagtcgaatc ccacattgcg ctctagtgct tgttatcctc cccacgaacc
     3541 tctgcagtgt acctggaagt gtgaatggaa agccccctga agtgcgacta aattctgcta
     3601 aagcgtgcag agcattgcga ggacggataa agtgactcca gttaccgtag gcattttgcc
     3661 tagctatagc attgcaggat cacgtggtat tatggggtag cgtcccttcg tcggctcagt
     3721 atacccggat atgccatcgt gcctcggctc tttcttgaca ggcggtcgat tctatcctta
     3781 tagttaacca ggttaatcaa ggggcgtgct agtaccatgg atcagctgct tcatatggcg
     3841 cagagcgtcg ccgtgttccg cagtatgtat atccgccatt ccaggagcga gcctggcggc
     3901 ggcggtagct ggcgcttcct catcctgatc cataacttcg acgaagggct gggcatcaag
     3961 cccgccgata tggcgcggta ctttcgctgc tcccgggcct acgtcagtaa gatgatcaag
     4021 gagctgacgg ctgacgggct cgtcgaggat cggccggacg agcgggacct gcggagccgc
     4081 tacctcgcct gtaccgagaa gggcgtacgg ttagcccggg ggatcatgga cgactacgtg
     4141 gcggtcacga ggcgcctgcg agacggtctc ggggtgcgta agagcgagca actcgtgtcg
     4201 ctcctcgacg aggccgtcgc gatactggaa cgcgttaacg atggggagga ccgcgaatag
     4261 tcgcgggctg gcggcgcatt ccgcggagcc tggctccagc gaagacgtgg tcgcagagga
     4321 atagggaacg gatctaccag gaggcgcctc gtgtcgagct ttaagaatct tcggatcgtc
     4381 gataacttct accagacctc gatgttctac cccatgccca ccatgctcat atcgacattg
     4441 tcgccgtccg gggctacctc gctcggcgcg tactcgctgt gcttcccgta ctacatcgcg
     4501 ggcaaggagt actacgccat gctcctggaa tgccgcaata gttcgaacac agcccagaac
     4561 ctcctgcgta cgggcgtctg cgccatcaat ttcctcgagg atagcaggcg gctcttcaag
     4621 gagacggtca ggctgggctt ccccggggag cgctgcgagg agaagatgag cgactgcttc
     4681 ctcgagatgg aggcggggca ggcggccgat aaggagtccg acggcggggc gcgccccctc
     4741 gtcctcaagg atgcctacca ggtgctggag tgcagc
//
LOCUS       DAOOLN010000014         4682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_22050_length_4682_cov_2.667603, whole genome shotgun sequence.
ACCESSION   DAOOLN010000014 DAOOLN010000000
VERSION     DAOOLN010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22050_length_4682_cov_2.667603"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PLW80_00370"
     CDS             complement(<1..502)
                     /locus_tag="PLW80_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HPB64982.1"
                     /translation="MAKNETIVANQTPDEKLKALEAARLQIEKQYGVGSLMQLGKYDN
                     PLGIEAIPSGSILLDEALGVGGYPRGRIIEIYGPESSGKTTLALHAIAEAQKKGGIAA
                     FIDAEHALDPVYAKNLGVNTDDLWVSQPDNGEQALEIAESLVRSGAVDIVVVDSVAAL
                     TPQAEIE"
     gene            complement(586..1887)
                     /locus_tag="PLW80_00375"
     CDS             complement(586..1887)
                     /locus_tag="PLW80_00375"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPB64983.1"
                     /translation="MTGSPIAFVASRFFFGRTAADGSRDQARKALAGAVLSVALSLVP
                     LITVMQVADGMIQGISARYVELGTYHAQALPYASADLVGARDAARSAPGVAGAWIETQ
                     SVGVAFANGEREGAAVRGVEPGFLEDPSTLAYLRVEAGEPTLERPYDALVGQAMAEKL
                     GLGPGDVVNLISMRKAADGELLPRVTMLTVRGVVSAGYRDLDAQWLFMRQDTAARVLP
                     KENTRSFVGIKADDPFGDPFAAQDAVEGRLPPGFTAYSWREVERNLFESLASTRTMLL
                     LIMAVTVAVAAVNVSSALQTLVLERGQEIAVMQSLGASPSDLARIFTLGGAAIGALGA
                     VLGAAGGLLASVRVNELLAFIEAVANSARSAAARLSGGAAATRFRVLDPAYYLETIPV
                     DVPYGEIAMVVAATVALSFVAALGPARRAASLSPLDGFRRR"
     gene            complement(1884..2564)
                     /locus_tag="PLW80_00380"
     CDS             complement(1884..2564)
                     /locus_tag="PLW80_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64984.1"
                     /translation="MSDDVIVCRGVRKVFASEAEDLEILRGVDLSLPSGAIASITGPS
                     GCGKSTFLSILGGLDRPSAGEASVAGVDLIRADEAGLSAFRATAVGFVFQFHYLLKDF
                     TALENAMLPAYMLTGNRAEAIGRARELLEAVGLGGRLDHGPAKLSGGERQRVAIARAL
                     VNAPGVVLADEPTGNLDRQSALAVEDLLFDLAARRGATLLVVTHDPGMAARADRRYIL
                     SDGTLVES"
     gene            complement(2557..3789)
                     /locus_tag="PLW80_00385"
     CDS             complement(2557..3789)
                     /locus_tag="PLW80_00385"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64985.1"
                     /translation="MRRRPWVLFVARRWFGSSDRTGPSLGPATAGIAVGVAALLCVIG
                     VMNGFQMGYIDAVLDLDSYHIRLPSGDAEAVLAALPDAEAAVPFVDLRTLAANRRGKA
                     AALRIKVLPDDALERDRSLAARMELRSGEYGGGVVVGSVLARQLDLRVGDTLSVLDVK
                     ADEIDGISTAMVDLTVSGVYHCGYFDYDAALVFLPESAAGELGRGEARVVGVKLRDRY
                     GDERAMATLAAAGLEGAESWRVYNRAFFGALRMEKSVMLLLVGLIFIVVGVNIFHSMR
                     KAVYGRVEDIATMKALGAGSSSVRRVFMLDGAAAGAGGAIIGLVVGLLVVTNVNGVFG
                     AIEAAVAAVYGALGGRGPGFEFFSPELFYIGDVPVRLSFPETLFIASAGALAAVAAAW
                     AASSRVSRIMPSEVLRDE"
     gene            complement(3786..>4682)
                     /locus_tag="PLW80_00390"
     CDS             complement(3786..>4682)
                     /locus_tag="PLW80_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64986.1"
                     /translation="VREAMEEGGSIREERLYDGDGQPSLERIFLAGGSVEETAYEYAA
                     GRLVSRTTSRDGAVVSTTSYLYAPDGRLAMARATAGAATGAAAGTAGGRASSAGGASS
                     GWTLGPLGLELRSYDGDGRLVRVALYVGAEEQRSEERTWAGGRLERVVVTEPSGARTE
                     TVYATDGPAAGEIVSLTERRGDVVAHVELRSYDRDGRLILVERSYGGTGASSGGTGAS
                     SGAAASTTAYEYDGSGELVSATTTVGGEIVTAVRYEPDGARVEESYDSGELFARVRYE
                     DGRRVLEELLERGQVIQTRSFE"
BASE COUNT          727 a         1694 c         1573 g          688 t
ORIGIN      
        1 cctcgatctc cgcctggggc gtcagggccg cgaccgagtc gacgacgacg atgtcgaccg
       61 cgccggagcg aacgagagac tccgctatct caagtgcctg ctcgccattg tccggctgcg
      121 acacccacag gtcgtcggta ttcacgccga ggttcttggc gtagaccggg tcgagcgcgt
      181 gctccgcgtc gatgaaggcc gcgatgccgc ccttcttctg ggcctcggcg atcgcgtgta
      241 gcgccagcgt ggtcttgccg gacgactccg gcccgtagat ctcgatgata cgcccgcgcg
      301 ggtagccgcc gacgccgagc gcctcgtcga gcaggatgga gccggacggg atagcctcga
      361 tgccgagcgg gttgtcatac ttgccgagct gcatgagcga gccgacgccg tactgcttct
      421 ctatctgcag gcgcgccgcc tcgagagcct tgagcttctc gtcgggggtc tggttcgcga
      481 ctatagtctc gttctttgcc atgtggtcct cctggtactg catacgcggc tacgatcacg
      541 aacgcgttcg cccggccact atagcgcggc ggggcggaat tgtcatcatc gcctgcggaa
      601 accgtcgagc ggagacagcg aggctgccct gcgtgccggc ccgagcgccg ccacgaagga
      661 cagcgcgacc gtcgcggcga cgaccatggc tatctcgccg tagggtacgt cgaccggtat
      721 cgtctcgagg tagtacgcgg ggtcgagcac tctgaagcgc gtcgcggccg cgccgcccga
      781 caggcgggcc gccgccgatc gggctgagtt ggccacggcc tcgatgaagg cgagcagctc
      841 gttgactcgg acgctcgcca ggagcccgcc cgccgcgccc aggaccgccc cgagcgcgcc
      901 tatcgccgcc ccgcccaggg tgaagatcct ggccaggtcg gacgggctcg ccccgaggct
      961 ctgcatgacg gctatctcct ggccccgctc aagcacgagg gtctggagcg cgctcgacac
     1021 gttgacggcc gcgaccgcga cggtgaccgc catgatcagg agcagcatcg tcctcgtgct
     1081 tgcgagcgac tcgaagagat tccgctccac ctcgcgccag gagtacgcgg tgaagccggg
     1141 aggcaggcgc ccctcgaccg cgtcttgggc ggcgaacggg tcgccgaacg ggtcgtcggc
     1201 cttgatcccg acgaagctac gggtattctc cttgggcagg acgcgggccg ccgtatcctg
     1261 ccgcatgaac agccattggg cgtccagatc tcgatagccg gccgacacca cgccgcgcac
     1321 ggtcagcatc gtgacgcgcg gcaggagctc gccgtccgcg gccttgcgca tcgatatcag
     1381 gttgaccacg tcgcccggtc cgagacccaa cttctcggcc atcgcctggc cgacgagggc
     1441 gtcgtacggc cgctcgagcg tcggctcgcc cgcctcgacg cgcagataag ccagggtcga
     1501 cgggtcctcg aggaagcccg gctcgacgcc gcgcacggcc gcgccctcgc gctcgccgtt
     1561 ggcgaacgcg acgccgaccg actgcgtctc gatccaagcg cccgcgacgc ccggagccga
     1621 gcgcgccgcg tccctggccc cgacgaggtc ggccgaggcg tacggcaggg cctgggcgtg
     1681 gtaggtgccg agctcgacgt accgggcgct gatgccctgg atcatgccgt cggcgacctg
     1741 catgaccgtg atgagcggca cgaggctcag cgcgaccgat agcacggctc cggctagggc
     1801 cttcctggcc tggtcgcggc ttccgtcggc ggccgtgcgc ccgaagaaga acctgctcgc
     1861 cacgaaggct atcggcgagc cggtcatgac tcgaccagcg tcccgtcgct cagtatgtac
     1921 cgccggtcgg ccctggccgc catgcccggg tcgtgggtga cgaccaatag ggtcgcgccg
     1981 cgcctggccg ccaggtcgaa cagcaggtcc tcgacggcca gagcgctctg cctgtccagg
     2041 ttgcccgtcg gctcgtcggc gagcacgact cccggagcgt tgacgagggc ccgggcgatg
     2101 gcgaccctct ggcgctcgcc gcccgacagc ttggccgggc cgtggtcgag ccgtccgccg
     2161 aggcccacgg cctcgagcag ttccctggcg cggccgatcg cctcggcgcg gttaccggtc
     2221 aacatgtagg ccggcagcat cgcgttctcg agcgcggtga agtccttgag caggtagtgg
     2281 aactggaaga cgaacccgac ggccgtagcc cggaacgccg acagcccggc ttcgtcggcc
     2341 cgtatcaggt cgacgccggc gaccgatgcc tctccagcgc tcgggcgatc gaggccgccg
     2401 agtatcgata ggaaggtact cttgccgcag ccgctcggac cggtgatcga agcgatggcg
     2461 ccgctcggca ggcttaggtc gaccccgcgc agtatctcca agtcttcggc ctcgctggcg
     2521 aacaccttgc gcacgccgcg gcagactatg acgtcgtcac tcatcgcgaa gcacctccga
     2581 aggcatgatg cgggaaaccc tggaactggc cgcccaggcc gcggcgacag cggcgagcgc
     2641 tccagccgac gctatgaaca gcgtctccgg aaagctgagc cgaaccggta cgtctcctat
     2701 atagaacagc tcaggcgaga agaactcgaa gcccggccct cgcccgccca aggcgccgta
     2761 gaccgccgcg accgcggcct cgatcgcgcc gaacacgccg ttgacgttcg tcaccacgag
     2821 cagcccgacg acgagtccta tgatggcccc gccggctccc gcggccgcgc cgtcgagcat
     2881 gaacacgcgc ctgacgctcg atgagccggc gccgagggcc ttcatcgtcg ctatgtcctc
     2941 gacgcgcccg tagaccgcct tgcgcatcga atggaagata ttgacgccga cgacgatgaa
     3001 gatgaggccg acgagtagca gcatcaccga tttctccatc ctgagcgcgc cgaagaacgc
     3061 gcggttgtag acgcgccacg attcggcgcc ctcgaggccg gcggcggcga gggtggccat
     3121 ggcgcgctcg tcgccgtagc ggtcgcgcag cttgacgccg acgacgcggg cctcaccgcg
     3181 gccaagctcg cccgccgccg actcgggcag gaagacgagc gccgcatcgt aatcgaagta
     3241 gccgcagtga tagacgcccg agacggtcag gtcgaccatc gcggtcgaga tgccgtcgat
     3301 ctcgtcggcc ttcacgtcga ggaccgacag cgtatcgccc acccggaggt cgagctgcct
     3361 ggcgagcacc gagccgacga cgacgccgcc gccgtactcg ccggaacgca actccatcct
     3421 ggcggccagc gagcggtcgc gctcgagcgc gtcgtcgggc aacaccttga tacgcagggc
     3481 ggccgccttg ccgcgacggt tggctgccag ggtccgcagg tcgacgaagg gcacggccgc
     3541 ctcagcgtcc ggtagcgcgg ccaacaccgc ctcggcgtcg ccggacggta gccgtatatg
     3601 gtagctgtcg aggtcgagca ccgcgtcgat gtaacccatc tggaagccgt tcatcacgcc
     3661 gataacgcag aggagcgcgg cgacgccgac ggctatgcca gcggtcgccg gccccaggga
     3721 cgggccggtc ctgtcgctcg agccgaacca gcgcctggcc acgaacagga cccacggtcg
     3781 tcgcctcact cgaagctcct cgtctggatc acctggccgc gctcgagcag ctcctcgagc
     3841 acgcgccggc cgtcctcgta gcgtacgcgg gcgaatagct cgcctgagtc gtacgactcc
     3901 tcgacgcgtg cgccgtccgg ctcgtaacga accgccgtga cgatctcgcc gccgaccgtg
     3961 gtggtggccg agaccagctc gcccgagccg tcgtactcgt aggcggtcgt cgacgccgca
     4021 gcgccggacg acgcgccggt accgccggac gacgctccgg taccgccgta cgaccgctcg
     4081 acgaggatca gccgaccgtc gcggtcgtag gagcgtagct cgacgtgagc cacgacgtcg
     4141 ccgcggcgtt cggtcagcga tacgatctcc ccggcggctg gcccgtcggt agcgtagacg
     4201 gtctccgtcc gcgctcctga cggctcggta acgacgacgc gctcgagtcg gccgccggcc
     4261 caggtacgct cctcactccg ttgctcctcg gcgccgacgt agagcgccac cctgactagc
     4321 cgaccgtcgc cgtcgtagga ccgtagctcg agtccgagcg gcccgagcgt ccagcccgac
     4381 gacgctccgc cggctgaaga cgctctaccg ccggcggtac cggcggcggc gcctgtggcg
     4441 gcgcctgccg tcgctcgggc catagccagc cgaccgtccg gcgcgtacag gtagctcgtc
     4501 gtcgatacga cggctccgtc gcggctcgtc gtcctcgaca cgagccttcc ggccgcgtac
     4561 tcgtaagccg tctcctcgac cgagccgccg gccaggaaga tacgctcgag gctcggctgc
     4621 ccgtctccgt cgtacagccg ttcctcgcgt atcgagccgc cctcctccat ggcctcgcgg
     4681 ac
//
LOCUS       DAOOLN010000015         4572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_22770_length_4572_cov_3.260350, whole genome shotgun sequence.
ACCESSION   DAOOLN010000015 DAOOLN010000000
VERSION     DAOOLN010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22770_length_4572_cov_3.260350"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1410)
                     /locus_tag="PLW80_00395"
     CDS             complement(115..1410)
                     /locus_tag="PLW80_00395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB64987.1"
                     /translation="MYLTKTREITRVGFVSTRFQGTDGVSLETWKWNEVLTELGYETF
                     FFSGKSDWFPERSMVVEDAFFGQPRVKELHDEIFDRSTRRRGLTAEVGEIKGRLKAKL
                     YEFVKEFNIDVLIVENAFAIPMHIPLGIALAELVAELEMPTVAHNHDFAWERQRFMTG
                     CAEDYIVRAFPPRLHSVYHVVINSEARRQLAFRCGISSVMIPNVWDFDAEPTEADEYN
                     AGLRSELGMGDEEVLFLQPTRIVARKGIESAIELVARYNGTVGPNQRGRLLISHPDLD
                     EGDEYQHRVLDYARFMDVDLIVRPDLIAVSRGERPDGGKVYSLWDTYVRADFVTYPST
                     YEGFGNAFLEAIFYRKPILVNRYAIYAQDIEPLGFRTTVMDGYVTDAVVKDVARVLSD
                     PMEERKRVAENFAIGKRYLSYEVLRRRLPTLFMNFGAVS"
     gene            complement(1401..2399)
                     /locus_tag="PLW80_00400"
     CDS             complement(1401..2399)
                     /locus_tag="PLW80_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPB64988.1"
                     /translation="MASASSLSEALIAELRRSIADNGIGRPQSPDGYERFLSPARLPS
                     ELSRLVGKAPRERPLAIAVDVYGTLLASAIGEIGPSAEWGETDDAAEEAAREAFPSDM
                     AERLRAIVEEDHARSRARGVPWPEVDAPSAFARALGLGLEDGARTCVAWECTINRCAP
                     MPGAAEFLAVCAKKGVPLGIVSNAQFYTRLFIEEAFESDLYRGEADEADEADEADEAD
                     DARPEGASGRPCLGFDPELALWSYETGRAKPDRWMFDELARRLSARGIPADRILYIGN
                     DALNDCAAAGEAGLMTALFAGDARSCRPRSGDDRVAANPPSLAAYSWDDLRRLLCT"
     gene            complement(2383..3789)
                     /locus_tag="PLW80_00405"
     CDS             complement(2383..3789)
                     /locus_tag="PLW80_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64989.1"
                     /translation="MRVLVVHYHLKPGGVTTVIRGQLAALASRGVEAALLVGEAPPDE
                     RWNGAPLAVDPSLAYDEPGSGGLPDAAKVAVLASTIQREAWRLASGGGDVVVHVHNPT
                     IRKNASLLPALSALARSGVRLALHVHDLAEDWRPDVYSGQPYPEGATWAAINRRDAEA
                     LSAAGAGSVRFLPNLLPRATAGEPPREPPAGPGLVLYPIRGIRRKNLGEALLLSLFMR
                     PGGSVGVTLPPNSPRDRPYYDGWKAAALELGAPVAFELGLARGLDELYGEARAVVTTS
                     VKEGFGLSYLEPAIRGRATLGRRLPWVVDDFEAEGLAFPALYGSIATPRGLFDREAFG
                     RRAAEVVREAARAYGLASEALPEAVVGRLMDGDTVDYGRLDELAQLEVLRSVARSPEA
                     RSAIISANPFIDGWDQAAEVAARIAPSALEPWSEAAYADRLDGLYAAALSGGGAAPDK
                     AALLGLYLRPEAFHGVGV"
     gene            complement(3786..>4572)
                     /locus_tag="PLW80_00410"
     CDS             complement(3786..>4572)
                     /locus_tag="PLW80_00410"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HPB64990.1"
                     /translation="VVIDSGSTDRTREVVAEYGAKVYLATDILPDIEPYRGKGENLWK
                     ALYVLEGDVIVYVDADIKNIHPRFVYGLVGPLLKNDDTRFVKAFYDRPIALDQKTVKP
                     SGGGRVTELVTRPLFSSFYPELAQFIQPLSGEYAGFRECFDTIPFPIGYGVETSMLMD
                     IYDRFGLEAMAQVDLDKRVHRNQSTVALGRMSFGIIQTFIAKLKASGKATMAGELYKT
                     MIQFELEGGAYRRKESMIDIVERRPIVELPEYLAKFPARAGKA"
BASE COUNT          722 a         1559 c         1541 g          750 t
ORIGIN      
        1 ttcctgccgc caggcctccc cctcgcgttc aggctctcgg gcggcgtcga ctggtcgccg
       61 gctccgaccg gctggaacct cggcctcgcc gtctcgggtg ggttctagcg gcgctcagga
      121 tacggcgccg aagttcatga acagcgtcgg caggcggcgc ctgagcacct cgtaggacag
      181 gtagcgcttg ccgatcgcga agttctcggc gacgcgcttc cgctcctcca tcgggtcgga
      241 tagcacacgg gccacgtcct tgacgaccgc gtcggtcacg tagccgtcca tcaccgtcgt
      301 cctgaagcct aggggctcga tgtcctgcgc gtaaatcgca tagcggttga ccagtatggg
      361 cttccggtag aagatcgcct cgaggaaggc gttgccgaag ccctcgtagg tcgacgggta
      421 cgtgacgaaa tctgcgcgca cataggtatc ccagagcgag tacaccttgc cgccgtcagg
      481 gcgctcgccg cggctcacgg ctatcaggtc cggcctcact atcaggtcga cgtccatgaa
      541 cctggcgtag tcgagcacgc ggtgctggta ctcgtcgccc tcgtcgaggt ccgggtgcga
      601 tatcaggagg cgcccccgct ggttcgggcc gaccgtgccg ttgtacctgg cgaccagctc
      661 gatggcgctc tcgatgccct tgcgggccac gatcctggtc ggctgaagga acagcacctc
      721 ctcgtctccc atgcccagct cggagcggag accggcgttg tattcgtcgg cctcggtcgg
      781 ctccgcatcg aagtcccaga cgttcgggat catcaccgac gagatgccgc accggaaggc
      841 gagctgcctg cgcgcttccg agttgatgac gacgtgatag acgctgtgca ggcgcggcgg
      901 gaaggcgcgg actatataat cctccgcgca gccggtcatg aagcgctggc gctcccaggc
      961 gaagtcatgg ttatgcgcca ccgtcggcat ctccagctcg gcgacgagct ccgcgagggc
     1021 gatgccgagc ggtatatgca tcggtatcgc gaaggcgttc tcgacgatca gcacatcgat
     1081 gttgaattcc ttgacgaact cgtacagctt ggccttgagc cggcccttta tctcgccgac
     1141 ctccgcggtc aggcctctac ggcgcgtcga ccggtcgaag atctcgtcat gcagctcctt
     1201 gacgcggggc tgcccgaaga aggcgtcctc cacgaccatc gagcgctccg ggaaccagtc
     1261 gcttttgccc gagaagaaga acgtctcgta gcctagctcc gtcagcacct cgttccattt
     1321 ccacgtctcg agcgatacgc cgtcagtccc ctggaaacgg gtcgacacga agccgacgcg
     1381 ggttatctcg cgcgtcttgg ttaggtacat agcagcctcc tcaggtcgtc ccatgaatac
     1441 gcggccagcg acgggggatt agccgccacc ctgtcgtccc cggagcgcgg cctgcaggaa
     1501 cgcgcgtccc ccgcgaacag ggccgtcatc aggcccgcct cccccgcggc ggcgcagtcg
     1561 ttgagcgcgt cgttgcctat atacaggatc ctgtccgccg ggatgccgcg ggcggacagg
     1621 cgtcgggcga gttcgtcgaa catccaacga tcgggtttgg cgcggcccgt ctcgtaggac
     1681 cagagcgcca gctcgggatc gaaaccgagg caaggccggc cggaggcgcc ttcgggccgc
     1741 gcgtcatccg cctcgtccgc ctcgtccgcc tcgtccgcct cgtccgcctc gccgcggtat
     1801 agatcggact caaaggcctc ctctatgaac aggcgcgtgt agaactgggc gttcgatacg
     1861 atgccgaggg gcaccccctt cttggcgcag acggctaaga actcggcggc accgggcatg
     1921 ggcgcgcagc ggtttatcgt gcattcccag gcgacgcagg tcctcgcccc atcctcgagc
     1981 cccagtccga gcgcgcgagc gaacgccgag ggggcgtcca cctcgggcca gggtacgccg
     2041 cgggcgcgcg agcgagcgtg gtcttcctcg acgatggcgc gcaggcgctc ggccatgtcg
     2101 ctagggaagg cctcacgggc ggcttcctca gcggcgtcgt cggtctcgcc ccattcggcg
     2161 ctcggcccga tctcgcctat ggcgctggcg agcagcgtgc cgtagacgtc gacggctatc
     2221 gccagcgggc gctctcgcgg cgccttgccg acgagacggg agagttcgga cggtaggcga
     2281 gccggggata ggaagcgttc gtagccgtcg ggagattgcg gccgtcctat cccgttatcg
     2341 gctatcgacc gcctaagctc ggcgatcagc gcctcgctca ggctagacgc cgacgccatg
     2401 aaaggcctcc ggcctgaggt acagcccgag cagcgcggcc ttgtcgggcg ccgcgccgcc
     2461 gccagacaag gccgctgcat acagcccatc gagccggtcg gcgtaggcgg cctccgacca
     2521 cggttcgagg gcgcttggcg cgattcgggc ggcaacctcg gccgcctggt cccagccatc
     2581 gatgaacgga ttggccgaga tgatagcgga gcgggcttcg ggcgagcggg cgacggagcg
     2641 caggacctcg agctgggcca gctcatcgag ccgcccgtaa tcgacggtgt ctccgtccat
     2701 caggcggccg accacggctt ccggcaacgc ctcagaggcg aggccgtagg cccgggcagc
     2761 ctcgcgcacg acctcggcgg ctcgacggcc gaaggcctcg cggtcgaaca ggccgcgcgg
     2821 agtagcgatg gagccgtaca gggccgggaa cgccaggccc tcggcctcga aatcgtcgac
     2881 gacccatggc aggcgtcgcc ccagggtcgc cctgccccgg atggccggtt ccaggtagga
     2941 caggccgaag ccttccttta ccgaggtcgt cacgacggcc ctcgcctcgc cgtacagctc
     3001 gtcgagccca cgcgcgaggc caagctcgaa ggcgaccggt gccccgagtt cgagcgcggc
     3061 ggccttccag ccgtcgtagt acggcctgtc gcgagggctg ttcggcggca gggtcacgcc
     3121 gacgctgccg cccggccgca tgaacagcga taggagcagg gcctcgccga ggttcttgcg
     3181 ccgtatgcca cggatggggt acaggaccag gcctggtccg gccgggggct cgcgcggcgg
     3241 ttccccggcc gtggcccggg gcaagagatt gggcaggaag cgtaccgaac ccgccccggc
     3301 ggccgatagg gcctcggcgt cgcgccggtt gatggcagcc caggtcgcgc cttcgggata
     3361 gggttggccg gagtacacgt cgggccgcca atcctcggcc aggtcgtgga catggagggc
     3421 caggcggacg cccgagcgag ccagcgccga tagcgccggc aggagggagg cgttcttacg
     3481 tatcgtcgga ttatggacgt ggaccacgac gtcaccgccg ccggacgcca gcctccaggc
     3541 ttctcgctgg atcgtcgagg ctagcaccgc taccttggcg gcgtccggaa gcccgcccga
     3601 tcccggctcg tcgtaggcca gcgacggatc gacggccaac ggcgccccgt tccagcgctc
     3661 gtccggaggc gcctcgccca cgaggagcgc cgcctcgacg ccccgcgagg cgagggccgc
     3721 gagctgcccc cgtatgaccg tggtcacgcc cccgggcttc aggtggtaat ggacgacgag
     3781 gaccctcatg ccttgccggc cctcgcgggg aatttggcca ggtactcggg cagctcgacg
     3841 ataggcctcc gctcgacgat gtcgatcatg ctctccttgc gccggtaggc gccgccctcg
     3901 agctcgaact ggatcatcgt cttatagagc tccccggcca tcgtcgcctt gcccgaagcc
     3961 ttgagcttgg ctatgaaggt ctggatgatg ccgaaggaca tgcggccgag cgccaccgtc
     4021 gactggttgc ggtgcacccg cttgtccagg tcgacctggg ccatcgcctc gaggccgaag
     4081 cggtcgtaga tgtccatcag catgctcgtc tcgacgccgt agcctatagg gaacggtatc
     4141 gtgtcgaagc actcgcggaa gcccgcgtac tcgcccgaca gcggctggat gaactgggcc
     4201 agctcgggat agaaactgga gaacagcggc ctcgttacca gctcggtgac tcgcccgccg
     4261 cccgaaggct tgacggtctt ctggtcgagg gctatggggc ggtcatagaa cgccttcacg
     4321 aagcgcgtgt cgtcgttctt gagcaagggg ccgacgagcc cgtacacgaa gcgcgggtgg
     4381 atgttcttga tgtccgcgtc cacgtagacg atgacgtccc cctcgagcac gtacagcgcc
     4441 ttccagaggt tctcgccctt gcccctgtac ggctcgatgt cgggcaggat atcggtggcc
     4501 aggtagacct tggccccgta ctcggccacc acctcacgcg tccggtccgt ggacccggag
     4561 tcgatcacga cg
//
LOCUS       DAOOLN010000016         4530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_23072_length_4530_cov_3.153966, whole genome shotgun sequence.
ACCESSION   DAOOLN010000016 DAOOLN010000000
VERSION     DAOOLN010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23072_length_4530_cov_3.153966"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PLW80_00415"
     CDS             <1..577
                     /locus_tag="PLW80_00415"
                     /inference="COORDINATES: protein motif:HMM:NF019244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HPB64991.1"
                     /translation="PCLIALFVALIVYGMIVDRDWRSSYRLLLGSSGRLVSDFVRFYG
                     LGATMVNMGLMGLMAMAAILLIGASWNGPMIGAALTIVGFSAFGKHPRNALPPMLGVA
                     FMASLSRYGLSSPSSQLAVLFSSTLAPVTGEYGFVPGFISGMLHLVLVQVVGSLHGGL
                     DLYNNGFAGGLAAGLFVPVLEWIREWRKHEA"
     gene            567..938
                     /locus_tag="PLW80_00420"
     CDS             567..938
                     /locus_tag="PLW80_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64992.1"
                     /translation="MKHDAAQLMRVAEEVLKYFYNKGARSVKVSYDFGDQRSFCAVHA
                     KDLVLSPEDEAYIQNVFEAPVQPEIASYYGILVGRRRDEQEMQLVGTMADLEDLQNEE
                     GLGVVIVLSRREMEFRSSKKG"
     gene            1018..1590
                     /locus_tag="PLW80_00425"
     CDS             1018..1590
                     /locus_tag="PLW80_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HPB64993.1"
                     /translation="MALTSRVATKVARRVVAAEGLVAAIGLVAAIGLVALLASMALAC
                     VPSGGRDEPAEAAAPRPSGKPQSGLATATLRSGAAEIVVELAVSPEEQEIGLMHRTEL
                     ADGRGMLFVYAEDRRLAFWMKHTLIPLSIAYISSDGVIREIRDMEPLSLAPVESERYV
                     RYALEAPLGWFGRVGLAPGDRFDLSGLPGR"
     gene            1642..2127
                     /gene="def"
                     /locus_tag="PLW80_00430"
     CDS             1642..2127
                     /gene="def"
                     /locus_tag="PLW80_00430"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPB64994.1"
                     /translation="MDIYTIGADVLRGNAAPVERFDDEFAALIERMFATMKQGKGVGL
                     AAPQVGIPQRFFVMQIDGDKQRVFVNPEIVETSLELDSFEEGCLSIPGVYADLDRPAE
                     IRVQAWNERGRRFVLGADGFLARVIQHELDHLNGVLFVDRLPERTRDRLLKQYEKRMR
                     A"
     gene            2133..3086
                     /gene="fmt"
                     /locus_tag="PLW80_00435"
     CDS             2133..3086
                     /gene="fmt"
                     /locus_tag="PLW80_00435"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPB64995.1"
                     /translation="MRVLFAGSPEIALPALEAIGARHELVGVLTNPPSAKGRGLCFSC
                     TPVAEAATALGVPVLSPERLGPAEREAVSSLRPELLAVFAYGRIFGPKFMALFPLGGI
                     NAHPSLLPRWRGCAPIPYAILNRDPVTGVCVQRVAPRMDAGDILARREIRLDGRETTD
                     SLSAVSATVGAELLLEAMAAIEAGTAVGEPQDEAAATYCGALAKDSGLVDWSSPAIDL
                     DARVRAFHPWPGAYTGFGGERLALLEAAPYPDEPGRAEPGTVLAVDKSKGIMVQTGRG
                     LIAVSRLQLRGKKPMDFKDFANGARGLVGTRLDAPPPSAAR"
     gene            3105..4115
                     /locus_tag="PLW80_00440"
     CDS             3105..4115
                     /locus_tag="PLW80_00440"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPB64996.1"
                     /translation="MDLRSMFNFKKLDQLDPRHAKAAIVSLVSLVVLMGLSGLIAFFL
                     ALRGEEQTLVPNVVDMELSAALVKLQDKELYPRVSLRFSGDPETRGRILEQDPQPGAI
                     VKAGRRIALVVSRGAAQEAVGDYIGQTVDEVKIHLQTVFGSTRQLITVKEPPVYVYDA
                     SPAGTILEQSPAPNVELSGPTQLSFVVSRGPEKAKVKVPDLVGLALSEAVLQIEKSGV
                     AFEFALRPTEGRERPGTVVAQLPAPGSLLDPGSPVSIVYGAPAKTEGRVSGLFQQALP
                     EYPYPLRAVLYAEPPTGARIPLISVDHPGRSFTMPYDLPVGSVLVLQVLNKVVARVEA
                     GR"
     gene            4112..>4530
                     /locus_tag="PLW80_00445"
     CDS             4112..>4530
                     /locus_tag="PLW80_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64997.1"
                     /translation="MSPRMIPSPRRRRKAPTYSVEIVRMALYDIGRSIDLKKAADLLP
                     GVPGMRLARRRDTPTTVTIPAPLCVDLRSGLKKVYSEDSIEGFAASARLYDEGVMSIV
                     VRLKVSATIEELHGIEAKEVSPTIRDLDSYIAERYQAV"
BASE COUNT          700 a         1518 c         1551 g          761 t
ORIGIN      
        1 gccctgcctg atcgccctgt tcgtcgcgct gatcgtctac ggcatgatcg tcgaccgcga
       61 ctggcgttcg tcctaccgcc tgctactggg atcctcggga cggctcgtca gcgacttcgt
      121 ccgcttctac ggactcggcg cgacgatggt caatatgggg ctgatggggc tgatggcgat
      181 ggccgcgatc ctgttgatag gcgcctcgtg gaacggccca atgataggcg ccgcgctgac
      241 catcgtcgga ttctccgctt tcgggaagca cccgcgcaac gccctgccgc cgatgctcgg
      301 cgtggcgttc atggcctcgc tgtcccgtta cggcctgtcg tcgccgtcca gccagctggc
      361 tgtgctcttc tcgagcaccc tggctcccgt tactggcgag tacggcttcg tgccgggctt
      421 catctccggg atgctgcacc tcgtgctcgt gcaggtggtc ggatcgctgc acggcgggct
      481 cgacctgtat aacaacggct tcgccggagg cctggcggcc ggcctcttcg tccccgtgct
      541 cgagtggatc cgcgaatgga ggaagcatga agcatgacgc agcgcagttg atgcgggtgg
      601 cggaggaagt gctcaaatat ttctataaca agggcgccag gtccgtaaag gtatcgtacg
      661 acttcggcga ccagcgctcg ttctgcgctg tgcacgcgaa ggacctggtc ctgtcgcccg
      721 aggacgaggc gtacatccag aacgtgttcg aggcgccggt gcagcccgag atagcgagct
      781 attacggcat cctggtcggc cgcaggcgcg acgagcagga gatgcagctg gtaggcacca
      841 tggcggacct cgaggatctg cagaacgagg aaggcctcgg cgtcgtcatc gtgctgagcc
      901 ggagggagat ggagttccga tcgtcgaaga aagggtagct tgccctgcgg gcgctaacgg
      961 tttatgctcg cctctcggga tggaggtgcc atgtatcagg tcggtcaaag gcgttcgatg
     1021 gcgctaacca gccgcgtcgc aaccaaggtc gcgcggagag tcgtcgcggc cgaaggcctc
     1081 gtcgcggcta taggcctcgt tgcggctata ggcctcgtcg cgctgctcgc gtcgatggcg
     1141 ctcgcctgcg tgccgtcggg cgggcgtgac gagccagccg aggccgccgc gccgaggccg
     1201 tccggcaagc cgcagtcggg cctggcgact gccaccctac gctcgggcgc ggcggagata
     1261 gtcgtcgagc tggcggtcag ccccgaggag caggagatag ggctgatgca tcgcacggag
     1321 ctggctgacg gccgcggcat gctgttcgtc tacgccgagg accgcaggct ggcgttctgg
     1381 atgaagcata ccctgatccc gctatcgatc gcctatatct cctcggatgg cgtcatccgc
     1441 gagatccgcg acatggagcc cctgtcgctc gccccggtcg agagcgagcg ctacgttcgc
     1501 tacgccctcg aggcgccgct cggctggttc ggccgcgtcg ggctcgcccc cggcgaccgc
     1561 ttcgacctgt ccgggctgcc cggtcggtag tcgaggcggc ggcgaagcca gtatgttcat
     1621 agtaggagag ccaaggccat gatggatata tatacgatag gagccgacgt gctacgcggg
     1681 aacgccgccc cggtggaacg cttcgacgac gagttcgcgg cgttgataga gcggatgttc
     1741 gcgacgatga agcagggcaa gggcgtcggc ctggccgcgc cgcaggtcgg tatcccgcag
     1801 cgcttcttcg tcatgcagat agacggcgac aagcaacgcg ttttcgtcaa cccggagatc
     1861 gtcgagacga gcctggagct ggacagcttc gaggaaggct gcctctcgat acccggcgtg
     1921 tacgccgacc tggaccggcc ggcggagata cgggtgcagg cctggaacga gcgcgggcgc
     1981 cgtttcgtgc tgggggcgga cggcttcctg gcccgggtca tccagcacga gctggaccac
     2041 ctgaacggcg tcctgttcgt cgaccggctg cccgagcgca cccgcgaccg cctgctcaag
     2101 cagtacgaga agcggatgag ggcctgacgc cggtgcgcgt cctgttcgcc ggtagccccg
     2161 agatagccct gccggcgctc gaggcgatag gggcccggca tgagctggtc ggcgtgctga
     2221 ccaatccgcc gtcagccaag gggcgaggct tgtgcttctc gtgcacgccg gtcgcggagg
     2281 ccgcgacggc cctcggcgtg ccggtgctgt cgcccgagcg cctggggccg gccgagcggg
     2341 aggccgtctc atcgctacgg cccgagctgc tcgccgtctt cgcgtacggc cgcatcttcg
     2401 ggcccaagtt catggcgctg ttcccgctcg gcggcatcaa cgcgcacccc tccctgctgc
     2461 cacgatggcg cggctgcgcg ccgattccct acgccatact gaatcgcgac ccggtcaccg
     2521 gggtctgcgt acagcgcgtc gcgccgcgca tggacgcagg ggacatcctg gcccggcgcg
     2581 agatccgctt ggacgggcgc gagacgaccg atagcctatc ggcggtctcg gccaccgtcg
     2641 gcgcggagct gctgctcgag gcgatggcgg ccatcgaggc cggtacggcg gtcggcgaac
     2701 cccaggacga ggcggccgcc acctactgcg gcgcgctcgc caaggactcg ggcctcgtcg
     2761 actggtcgtc gccggccatc gacctggacg cgcgcgtgcg agccttccat ccctggccgg
     2821 gcgcttatac cggcttcggg ggcgagcggc tcgccctcct cgaagcggcg ccgtacccgg
     2881 acgagcccgg ccgggcggag cccgggacgg tacttgcggt agacaagtcg aaaggtataa
     2941 tggtacaaac gggacggggc ctcatcgccg tgtcgcgcct ccagcttcgc ggcaagaagc
     3001 ccatggactt caaggacttc gcgaatggcg cgcgcggctt ggtcggtacg cgcctggacg
     3061 cgcctccgcc gagcgccgcc cgataaaccg gtacgggagc acgcatggac cttcgttcga
     3121 tgttcaactt caagaagctc gatcagctcg atcccagaca cgccaaggcg gccatcgtgt
     3181 cgctcgtgtc gctcgtggtg ctgatgggct tgtccggcct catcgcgttc ttcctggccc
     3241 tgcgcggcga ggaacagacg ctcgtgccga acgtcgtcga tatggagctg tccgcggccc
     3301 tcgtcaagct ccaggacaag gagctgtatc cgcgggtatc cctgcgcttc tccggcgacc
     3361 ccgagacccg agggcgcatc ctcgaacagg atccgcagcc gggagccatc gtcaaggctg
     3421 gtcgccgcat cgcgctggtc gtctccaggg gcgccgccca ggaggccgtc ggcgactata
     3481 tcgggcaaac ggtcgacgag gtgaagatcc acctccagac ggtgttcggg tcgacgcgcc
     3541 agctcataac cgtcaaggaa ccgccggtct atgtctacga cgccagcccg gcaggcacca
     3601 tactcgagca gtcgccggcg cctaacgtgg agctgtccgg cccgacccag ctgagcttcg
     3661 tcgtatcccg cggccccgag aaggccaagg tcaaggtgcc ggacctcgtc ggcctggcgc
     3721 tgagcgaggc cgtgctgcag atcgagaagt cgggcgtggc cttcgagttc gcgctgcggc
     3781 cgaccgaggg gcgcgaacgg cccggcaccg tcgtagccca gctgcccgcg ccgggtagcc
     3841 tgctcgaccc cggctcgccg gtatccatcg tctacggggc gcccgccaag accgagggcc
     3901 gcgtctccgg gctgttccag caggccctgc cggagtatcc gtaccccctg cgggccgtct
     3961 tgtacgccga gcccccgacc ggcgccagga tcccgctgat ctcggtcgac catccgggcc
     4021 gttccttcac gatgccgtac gacctgcccg tgggcagcgt gctcgtgctc caggtgctca
     4081 acaaggtggt ggctcgggtg gaggcgggaa ggtgagcccg cggatgattc cctcgcccag
     4141 gcggaggcgc aaggccccga cctattccgt cgagatcgtc cgcatggccc tctacgatat
     4201 cggccgctcc atcgacctca agaaggcggc cgacctgctg cccggcgttc cgggtatgcg
     4261 cctggccagg cgccgcgata ccccgacgac cgttacgatc cccgcgccgc tttgcgtcga
     4321 tctgcgctcg ggcctcaaga aggtgtatag cgaggattcc atcgagggct tcgccgcctc
     4381 cgcgcgcctg tacgacgagg gcgtcatgtc catcgtcgtc aggctcaagg tcagcgccac
     4441 catcgaagag ctacacggca tcgaggcgaa ggaggtttct ccgaccatcc gcgacctcga
     4501 ctcctacata gcggagcgct accaggccgt
//
LOCUS       DAOOLN010000017         4479 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_23410_length_4479_cov_3.126808, whole genome shotgun sequence.
ACCESSION   DAOOLN010000017 DAOOLN010000000
VERSION     DAOOLN010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23410_length_4479_cov_3.126808"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..884
                     /locus_tag="PLW80_00450"
     CDS             102..884
                     /locus_tag="PLW80_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64998.1"
                     /translation="MSGASAAAPKRPLTAPSMPTWLYASLGAFLALAYAALVAPGRFE
                     AIAGLGGPSELILFWLDFGLPIVLLAAMLIAAGPRRLEGSMALAMLAMLAAAASGSLY
                     ALSGRLEFVPEAVKQAASPTGVVVSLIWVGRAGLYPGDEALVEAPVGRRATIATVSVA
                     LVVALALYARPDGARGYYALALLASQTLVVAIAALRFRRLSAAWLAPLVAYWLAYLAA
                     YAAGLLPEAAAAWIPAAWRHAPSVCAVAAIAALSLSDAEARP"
     gene            884..1552
                     /locus_tag="PLW80_00455"
     CDS             884..1552
                     /locus_tag="PLW80_00455"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HPB64999.1"
                     /translation="MMIQVYLSFVVPLAFLLLVVTGEGRGVMASFAFGLSAALAVYGL
                     TGFISEAVVPLPMQLAFVIPAIEEAAKVLPLVYLLLRSKKTGKYSLVRYAMAIGIGFS
                     ILENYMYLTMAADSGVSSTIVFTLLRSLTASLLHGSTTALSGFCFQLMVNRSRFSPAL
                     ALGTLLGGCAVHSLYNALLLNAEHGAWAIVLPIASFGFVLFGVNGFGIVGSPRTRRKE
                     GASA"
     gene            1549..3426
                     /locus_tag="PLW80_00460"
     CDS             1549..3426
                     /locus_tag="PLW80_00460"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPB65000.1"
                     /translation="MRRIRTIATAGLALAVMLVAAACQAPVWENFGLLEFPGLEAYRG
                     PLGGRLGEVAFGDILVGEGDSYTLSMRNASSKPLTIDRIAFDSPHYDFYNHVSPAFPI
                     TLAAGETSDLLTVGFYPTDNGDLDGRVMFEVADRVVSVGLTGRGVWQLTLSIHPDTLD
                     EGFDAGQITSPVTVTPASGAVTFLSADGVVKLTALANIMSKFNAWEVLGTPKVEPDFA
                     DDNLKAEDTIVTLRAHTAIQASFYNPYVYFPVAGATPVGGTAYDSLQAAIDACVPSPT
                     LEAVIIKRGSHTVGTGVTLPEGVSIFGGYSADYATRAYKTPAERSTTNPAILTFAAGT
                     GLSVSAASGQQSVIEGVTLTRSAASATPVITVGSGAKPVIRYTTITTQGEGPALQSSY
                     GSPRIGDCVITGGAGAGKSVGAYFYGGAPYLEWNDIRSGNTSGNYSEAYAVRFEDNSY
                     PDLYMNTITAGTASGTKSASYGVYLDFGCRPLIVGNTIDGGRAPGSGGISTGVFYINK
                     DGKATVRYNTISGGFGALQARALYAAQGANYYLRYNNLTADQASGSTVGLYLDTNGRV
                     RRLIGNGFVGLGTLQYTWLSDEAIGDIDELNGGYYLSDTDANKAEAISLPGVPSLGVR
                     E"
     gene            3423..4409
                     /locus_tag="PLW80_00465"
     CDS             3423..4409
                     /locus_tag="PLW80_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65001.1"
                     /translation="MSTTNRRYARTIVAALAATACALVSACPTPYQGAIDIESRVIRV
                     DLPDVIFNPFSASAPATVRGVGAISAVVTVTTDADSYEAIIGSGGLDAVYDYTQAAVT
                     VTGRLVSVSVAVSTVSGQTFTAELDDWKDGSAYAGWTTFANVRKFRLCLKPGVETVTG
                     SALSLEPGPRGGVGLRLLYDNHATDPDFAYTDRGQAAALGSGVITVQPVWLENPAGLS
                     YDVHSLVVNENLQALYDPLYSSAAEYWPDAWTGSWTVDEIRSATGKSVAGSKVALVRQ
                     VTNLSAVNPLGDGNSSYRFSYPAYVGKYLVVALVVTDGQLSVPTQAVVIPIE"
BASE COUNT          701 a         1585 c         1423 g          770 t
ORIGIN      
        1 gaggcgaccg tggtggtcgg tgacgacggc gtcggcagcg tcgacctgac cggctcgagc
       61 gaactgtccc tgagcctcgg cctctcgttg gtgatacgcc gatgagcgga gcctccgcgg
      121 ccgcgccgaa gcggccgctc accgcgccgt cgatgccgac ctggctgtac gctagcctcg
      181 gggccttcct ggccctggcc tacgcggcgc tggtcgcgcc tggaagattc gaggcgatcg
      241 ccgggctcgg cggaccgtcc gagctgatac tgttctggct cgacttcggg ctgccgatcg
      301 tcttgctggc cgcgatgctg atcgcggccg gcccgaggcg tcttgaaggc tcgatggcgc
      361 tcgcgatgct ggcgatgctc gcggcggccg cctcgggctc gctctatgcg ctgtccgggc
      421 ggctcgagtt cgtacccgag gcggtcaaac aggcggcctc gccgaccggc gtcgtcgtct
      481 cgctgatctg ggtgggtagg gccggcctct acccgggcga cgaagcgctc gtagaggcgc
      541 ccgtcggacg gcgcgcgacg atagccaccg tctcggtggc gctcgtcgtc gccttggcgc
      601 tctacgccag accagacggc gcccggggct actacgcgct cgctctcctc gcgtcgcaga
      661 ccctcgtcgt cgcgatcgcg gcgctacgct tcaggcggct gtcggcggcc tggctggcgc
      721 cgctcgtcgc gtactggctg gcttacctgg ccgcgtacgc cgccggcctc ctccccgagg
      781 cggccgccgc ctggatcccg gccgcttggc gccacgcccc gtcggtctgc gccgtcgccg
      841 cgatcgcggc cctatccctg tcggacgcgg aggcccgccc gtgatgatga tacaggtcta
      901 cctgagcttc gtggtgccgc tggccttcct cctcctcgtc gtgaccggcg aagggcgggg
      961 ggtgatggcc tcgttcgcgt tcgggctgag cgcggccctg gcggtctacg gcctgaccgg
     1021 cttcataagc gaggccgtcg tgcccctgcc tatgcagctg gccttcgtca tcccggccat
     1081 cgaggaggcg gccaaggtcc tgccgctcgt ctacctgctc ctgcggagca agaagacggg
     1141 caagtactct ctggtacgct acgcgatggc cataggcata ggcttctcca tactggagaa
     1201 ctacatgtac ctgaccatgg cggccgactc gggcgtctcg tcgaccatcg tctttaccct
     1261 gctacgtagc ctgaccgcct cgctcctgca cggctcgacg acggccctct ccggcttctg
     1321 tttccagctg atggtcaatc gatccagatt ctcgccggct ctggcgctgg gcaccctgct
     1381 cggcggctgc gccgttcatt ccttgtacaa cgcgctgctc ctgaacgccg agcacggcgc
     1441 ctgggccatc gtgctgccga tagcgagctt cggcttcgtg ctgttcggcg tcaacggctt
     1501 cggcatcgtc ggctcaccca ggacccggcg caaggaagga gcctccgcat gaggcgaata
     1561 cgcactatcg cgaccgccgg cctcgcgctg gccgtcatgc tcgtggccgc ggcctgccag
     1621 gcccccgtct gggagaactt cggcctgctc gagttccccg gtctcgaggc ctatcgcggt
     1681 ccactgggcg gccggctcgg cgaggtagcc ttcggcgata tactcgtggg agaaggcgat
     1741 tcgtacaccc tctcgatgcg caacgccagc tccaagccgc tgaccataga ccgcatcgcc
     1801 ttcgactcgc cgcactacga tttctataac cacgtctctc cggccttccc cataaccctc
     1861 gcggccggcg agacgagcga cctcctgacc gtaggcttct atccgaccga caacggcgac
     1921 ctggacggcc gcgtgatgtt cgaggtggcc gaccgcgtcg tctccgtggg cctgaccggc
     1981 cgaggggtgt ggcagctgac gttgtcgata cacccggata cgctcgatga aggcttcgac
     2041 gccggtcaga tcacgtcgcc ggtcacggtt actccggctt caggtgcggt cacgttttta
     2101 tcggccgacg gagtcgtcaa gctgacggcc ttggccaata tcatgtcgaa gttcaatgcc
     2161 tgggaagttc tgggcacgcc gaaagtagaa ccggacttcg ccgatgataa cctgaaggcc
     2221 gaggacacga tcgtcacgct ccgcgcgcat accgccatcc aggccagctt ctataatccg
     2281 tacgtctact tccccgtcgc cggagccacg cctgtaggcg gaaccgcgta cgacagtctg
     2341 caagccgcga tcgatgcctg tgtcccaagc ccgaccctcg aagccgtcat aataaagcgc
     2401 gggtcgcaca cggtcggcac cggcgtcacc ctgcccgagg gcgtctcgat cttcggcggc
     2461 tacagcgccg attatgcgac acgggcgtac aagacgcccg ccgaacggtc cacgaccaac
     2521 cccgctatcc ttaccttcgc ggccggcact ggcctctccg tatcggccgc ctccggccag
     2581 cagagcgtca tcgagggcgt cacgctgaca cgctcggccg cgagcgccac gccggtgatc
     2641 acggtcggct ccggggctaa gccggtcatc cgctacacga cgatcacgac gcagggcgaa
     2701 ggtccggcgc tgcagtcgag ctacggcagc ccgcgcatcg gcgactgcgt gatcaccgga
     2761 ggcgcgggtg ccggcaagtc ggtcggcgcc tacttctacg gcggcgcgcc gtacctggag
     2821 tggaacgaca tacgctcggg caacacctcg ggtaattact ccgaggccta cgccgtgcgc
     2881 ttcgaggaca attcctatcc cgacctttat atgaacacga tcacggccgg caccgcgagc
     2941 ggcaccaagt cggcgtcgta cggcgtgtac ctggacttcg gctgcaggcc cctgatcgtc
     3001 ggcaacacga tagacggcgg acgcgctccg ggctccggcg gcatcagcac cggcgtcttc
     3061 tacatcaaca aggacggcaa ggcgacggtc cgctacaata cgatcagcgg cggcttcggc
     3121 gcgctccagg cccgcgccct gtacgccgcc cagggcgcca actactacct gcgctacaac
     3181 aacctgacgg ccgaccaggc ctcgggttcg accgtcggcc tctacctgga cacgaacgga
     3241 cgggtgcgcc gcctcatcgg caacggcttc gtcgggctcg gcaccctaca gtacacctgg
     3301 ctgagcgacg aggctatcgg cgacatcgac gagctgaacg gcggctacta cctgagcgac
     3361 accgacgcga acaaggccga ggcgataagc ttgcccggcg tgccttcgtt gggggtgagg
     3421 gaatgagtac gacgaatcga cgatatgccc gtacgatagt cgccgccctg gcggcgaccg
     3481 cctgcgcgct agtatccgcc tgcccgacgc cctaccaggg cgcgatagac atcgagtcgc
     3541 gcgtcatccg ggtcgatctg ccggacgtga tcttcaaccc gttctccgcg agcgcgccgg
     3601 cgacggtccg aggcgtcgga gccatctcgg ccgtcgtcac ggtcacgacc gacgctgaca
     3661 gttacgaagc tattatcggc tcgggcggcc tcgacgccgt ctacgactat acccaggcgg
     3721 ccgtcacggt aacgggccgg ctcgtgtcgg tgagcgtcgc cgtctccacc gtcagcggcc
     3781 aaaccttcac ggccgagctc gacgactgga aggacggctc ggcctacgct ggctggacca
     3841 ccttcgccaa cgtacgcaag ttcagactct gcctcaagcc cggcgtcgag accgtcaccg
     3901 gctccgctct gtcgctcgag cccggccctc gcggcggcgt cggcctcagg ctgctctacg
     3961 ataaccacgc gaccgacccc gacttcgcct acaccgaccg cggccaggcc gcggctctcg
     4021 gctccggcgt cataaccgtg caacctgtct ggctcgagaa ccccgcgggc cttagctacg
     4081 acgtgcacag cctcgtcgtg aacgagaacc tccaggcgct ctacgacccc ctgtacagca
     4141 gcgccgcgga gtattggccg gacgcctgga ccggttcgtg gaccgtggac gagatccgtt
     4201 cagcgacggg caagtcagta gccgggtcga aagtggcgct cgtccgccag gtgaccaacc
     4261 tgagcgccgt gaatccactc ggcgacggca acagctcgta ccgcttctcg taccccgctt
     4321 acgtcggcaa gtacctcgtg gtggccctgg tcgtcaccga cgggcagctg tcggtaccga
     4381 cccaggcggt ggtcataccg atagagtaat cgcgacgagt acgtcacgtc gcgacgagta
     4441 cgtcacgtcg tgacgagtac gtcacgatgg cgcgggaat
//
LOCUS       DAOOLN010000018         4437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_23676_length_4437_cov_3.137608, whole genome shotgun sequence.
ACCESSION   DAOOLN010000018 DAOOLN010000000
VERSION     DAOOLN010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23676_length_4437_cov_3.137608"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PLW80_00470"
     CDS             complement(<1..360)
                     /locus_tag="PLW80_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPB65002.1"
                     /translation="MRSIAGVSVRDGRVFVARRSPGGSMGGTWEFPGGKCEPGESDAE
                     AVVREYDEEFGLRVVPGPTIGESGFVNGVKPYELAAVMVEFDGEPPALREHDEYRWVG
                     AEELVALELSDSDRSLLA"
     gene            complement(361..771)
                     /locus_tag="PLW80_00475"
     CDS             complement(361..771)
                     /locus_tag="PLW80_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65003.1"
                     /translation="MVQFYLLSVLMNIVSGYALVSTDAEPRGTKMDGIREFLRDSTIR
                     LVLGILCASVGFFKLLTVMRGDVPVVGDLVPSLAGLAAGFSLLLEFYKSNSNVTTPAL
                     ERLDAIFVAQRRVVGIVAIVSGFVHFLFGNVLFL"
     gene            complement(858..1385)
                     /locus_tag="PLW80_00480"
     CDS             complement(858..1385)
                     /locus_tag="PLW80_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65004.1"
                     /translation="METKDKGQAVYYYDRAKRMEKAGPYARFMAEHYGAKRPGILRSL
                     TATRPLKSLFLALMFMLVAVLVVGYVQGAKNSGSVAGSRLVSTAMWFDGHVYVTVKRK
                     PTFFGRDAGPQDRLAIEIAAGDGSAHAVAVLQAADDETRLRFEAASKPEAVAVLATAG
                     SGDDASRLEMLVKVE"
     gene            complement(1369..2907)
                     /locus_tag="PLW80_00485"
     CDS             complement(1369..2907)
                     /locus_tag="PLW80_00485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPB65005.1"
                     /translation="MRPLVGFASALALDASARDGLGDDLLMEAAAVGMAAAIDADPAL
                     RKAAASGSPSIAAVCGGGNNAGDALAVLRRLAFSGWKDLVALVPDSLGPLASRRLAEA
                     GLAGARVIGLGDDAAHEAVARAGVVLDGLSGVGFKGPARAALVQAAALIASARGPVVS
                     MDVPSGVGALGSVAADPSAPVCADITLCVEPLKAELYYPGFRPYAGRILPIGGVFDRS
                     AGAASTMVLLEPGDLEDALPGLDPDCHKGDRGAVCAFAGSYGSTGAAVICSRAAAAAG
                     AGSVTLLARDELVPMLSTMLVAQMVRPESDPGTRRFTAALAGPGWGADERNAARLESL
                     WDAALPLALDADALGLLATSGLARRCSPLLLTPHPGEFAPLAAVAAGAQPDDPAALEL
                     AARRTRFDTAAVVAETAAYFGAVVALKGSVTWIGDPDGRLAVWDGREPALATAGSGDV
                     LAGLAAGFLARGASAWDAAKAAVIAHGLAGRALARRGFFEADALLPEVARLSYGRGIN
                     GNQG"
     gene            complement(2904..4118)
                     /locus_tag="PLW80_00490"
     CDS             complement(2904..4118)
                     /locus_tag="PLW80_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65006.1"
                     /translation="MVGKRLNVGLIAATLVAMLASCSDAREVTALQREQLFTLGYGVL
                     EDQLNLFSLEGNAPPAKTALAMRDGIFFVSNGNAAKVMTLSSFGDLLAMVYNPERNPP
                     PLTLQAAETGAVAQGRVARAYPFGSPGEIAVDSKRTIFVEDRVPDSRRSYDDSSMASL
                     EYVVLRFSRDGEYLDYLGQEGVGGTPFPSIVGLYITAADDCVVVCLTGMGWTAFVFDR
                     FGILESTVSIRRNELPRPPEEAGGIASLDRIVAAPDGSGLIIKIDYYREIVDQETHTQ
                     AGIEFSSSWAWVMDGSNGAYVERYELQPFESLVKDEARPVPRAWGLVGAAADTLFLAA
                     TDDDGATYYGLFDRVSRTIRRYSLKIEPDELLYASFSLSPEGILSAVLGSRYEARFVW
                     WRFDRLIGGLAP"
     gene            4207..>4437
                     /locus_tag="PLW80_00495"
     CDS             4207..>4437
                     /locus_tag="PLW80_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65007.1"
                     /translation="MAFNPLTALFGSKRERDIKALLPRLHAVNEREAWAMALKPEEFP
                     AKTAELKARVASGEPLDSVLPEAFALAREAATA"
BASE COUNT          804 a         1542 c         1463 g          628 t
ORIGIN      
        1 cgcgagcagc gagcggtcgg agtcggacag ctcgagcgct acgagctcct cggcgcctac
       61 ccagcgatac tcgtcgtgct cgcggagcgc cggcggctcg ccgtcgaact cgaccatgac
      121 ggcggccagc tcgtatggct tgacgccgtt gacgaagccg gactcgccta tcgtcggccc
      181 cggcacgacg cgcaggccga attcctcgtc gtactccctg accacagcct ctgcgtcgct
      241 ctcgcctggt tcgcatttac cgcccgggaa ctcccaggtg cctcccatgg agcctcccgg
      301 cgacctgcgg gctacgaaaa cgcggccatc gcgaacggag acgccggcta tcgaccgcat
      361 ttataggaag agtacgttcc cgaacagaaa atgaacgaag ccgctgacga tggccacgat
      421 gccgacaaca cggcgctgcg cgacgaagat ggcgtccagc cgctcgaggg ccggggtcgt
      481 cacattcgag ttgctcttgt agaattcgag caacagggag aaaccggccg cgagaccggc
      541 caacgacggc acgaggtcgc cgacgacggg gacgtcgccg cgcatgacgg tcagtagttt
      601 aaagaaaccg accgaggcgc acagtatccc caggacgagg cggatggtgc tgtcgcggag
      661 gaactcgcgg ataccgtcca ttttggtacc ccgcggttcg gcgtcggtcg agaccagagc
      721 gtagcccgag acgatgttca tcaggacact caagagatag aactgaacca tgccgacttc
      781 tcccttcagc ctcgttaggt accataatga aaagcccggg agcggtcaag gcgacgccgg
      841 accgacctca ctcgaactca ctcgaccttg accagcatct ccagcctcga cgcgtcatcg
      901 ccgcttccgg cggtcgcgag cacggccacc gcttccggct tgctggcggc ctcgaaacgc
      961 agccgcgtct catcgtctgc cgcctgcagt accgctacgg cgtgagccga gccgtccccc
     1021 gcggctatct ctatcgccag cctatcctgc ggcccggcgt cccgcccgaa gaaggtcggc
     1081 ttccgcttga cggtaacgta gacgtggccg tcgaaccaca tggccgtcga gaccagacgc
     1141 gagccggcga cggagcccga gttcttcgcc ccctggacat agccgacgac gagtaccgcg
     1201 acgagcatga acatgagcgc caggaacagc gacttgagcg gcctggtcgc cgtcaacgag
     1261 cgcagtatcc ccgggcgctt ggctccatag tgctcggcca tgaagcgggc atacggcccg
     1321 gccttctcca tacgtttagc ccggtcatag tagtagactg cctggccctt atccttggtt
     1381 tccattaatt ccccttccgt atgacagcct ggcgacctcg ggcaagaggg cgtcggcctc
     1441 gaagaacccg cggcgcgcca gggcgcggcc ggctaggccg tgggcgatga ccgcggcctt
     1501 ggccgcgtcc caggcggaag ccccccgagc cagaaagccc gcggctagcc ccgcgagcac
     1561 gtcaccggag ccggcggtcg ctagcgcagg ctcgcggccg tcccagaccg ccagcctgcc
     1621 gtcggggtcg cctatccagg ttacggaccc cttgagagcg acgactgccc cgaaatacgc
     1681 cgccgtctcg gcgaccacgg cggccgtgtc gaaacgggtc cgacgcgccg ccagctcaag
     1741 ggccgccggg tcatcgggct gggcgcccgc ggcgacggcc gccagcgggg cgaactcgcc
     1801 aggatgagga gtcaggagta gaggcgaaca gcgccgcgcc aagccggaag tcgcaagcag
     1861 cccgagcgcg tcggcgtcca gggccagcgg cagggccgcg tcccacagcg actcgagccg
     1921 cgcggcgttc cgctcgtcgg cgccccagcc cggcccggcc agcgccgcgg tgaagcgcct
     1981 ggtgcccgga tcggactccg gccggaccat ctgcgccacg agcatcgtcg agagcatcgg
     2041 caccagctcg tcgcgtgcca gcaacgtgac gctaccggcg ccggccgcgg ccgcggccct
     2101 ggaacagatc acggccgcgc cggtagagcc ataggaaccg gcgaaagcac agacggctcc
     2161 gcgatcgccc ttgtgacagt ccggatcgag tcctggcagg gcgtcttcga ggtcgccggg
     2221 ctcgaggagg accatcgtcg atgccgcacc ggccgatcga tcgaacactc caccgattgg
     2281 caggatgcgg cccgcgtagg gccggaagcc cgggtagtac agctccgcct tgagcggctc
     2341 gacgcacagc gttatatcgg cgcataccgg tgcgctgggg tcggctgcga cagacccgag
     2401 cgcgccgacg ccggaaggca cgtccatcga tacgacaggc ccccgagccg acgcgatcag
     2461 ggcggccgcc tgaacgaggg ccgctcgagc gggccccttg aagccgactc ccgatagccc
     2521 gtcgagcacc acgccggctc tcgcgacggc ttcatgagcc gcgtcgtcgc cgaggccgat
     2581 aacgcgagcg cccgccagcc cggcctcggc tagccgccgc gaggccaggg ggccgaggct
     2641 atcgggtacg agcgcgacca ggtccttcca gccggaaaag gccaggcgcc ggagaacggc
     2701 cagcgcgtct ccggcattgt taccgccgcc gcagacggcc gcgatggacg gcgaacccga
     2761 ggccgccgct ttcctcaagg ctggatcggc atcgatcgcg gcggccatac cgacggcggc
     2821 ggcctccatc aggagatcgt cgccaaggcc atccctcgcc gacgcgtcga gcgccagcgc
     2881 cgaggcgaag ccgacgagcg gcctcacggc gcgagcccac ctatcaggcg gtcgaagcgc
     2941 caccagacga agcgagcctc gtaacgggag ccgagcacgg cggagagtat accctccggg
     3001 gacagcgaga acgaagcgta caagagttcg tcgggctcga tcttcagcga gtagcgccgt
     3061 atcgtgcgcg aaacccggtc gaacagcccg tagtaggtgg ccccgtcgtc gtcagtcgcc
     3121 gccaggaaca gcgtgtcggc cgcggccccg accaggcccc aggctctcgg taccggccgc
     3181 gcctcgtcct tgaccaggga ttcgaagggc tggagctcgt agcgctcgac gtaggcgccg
     3241 ttcgaaccgt ccatgaccca ggcccatgac gacgagaact cgatgccagc ctgggtatgg
     3301 gtctcctggt cgactatctc cctatagtaa tcgatcttga tgatcaggcc ggagccgtct
     3361 ggcgccgcga cgatccgatc gagcgaggcg atgcctcccg cctcttcagg cggcctcggt
     3421 agctcgttac ggcgtatcga gaccgtcgac tccaggatgc cgaagcggtc gaagacgaag
     3481 gccgtccagc ccatgccggt caggcagacg acgacgcaat cgtccgccgc ggtgatgtag
     3541 aggccgacga tgctcgggaa cggcgtaccc ccgacgccct cctggccgag gtaatcgagg
     3601 tactcgccgt cgcgggaaaa gcgcaacacg acgtactcga ggctggccat cgacgagtcg
     3661 tcgtacgacc ggcgggaatc gggcacgcgg tcctcgacaa agatggttcg cttcgagtcg
     3721 accgctatct cgccgggcga gccgaacgga taggcccggg cgacgcggcc ctgggctacc
     3781 gcgccggtct ccgccgcctg cagggtcagg ggcggcgggt tacgctctgg attatagacc
     3841 atcgcgagga ggtcgccgaa cgacgacagg gtcatgacct tggcggcgtt gccgttcgag
     3901 acgaagaaga tgccgtcgcg catcgccagg gcggtcttcg ctggcggcgc gttgccctcg
     3961 aggctgaaca ggttgagctg gtcctcgagc acgccgtagc cgagggtgaa gagctgttcg
     4021 cgctgcaacg ccgtaacctc gcgcgcgtcg gagcaggaag cgagcatagc caccagcgtg
     4081 gccgcgatca aacctacgtt gagcctcttg cctaccacgc cgcgacctcc ggatcgagaa
     4141 tcggttccgc ccgaccgccc gggtcttgct tgaccgctta tcgttaaaag atatagatta
     4201 aacgacatgg cgttcaaccc cctgactgct ctcttcggct cgaagcgaga acgagacata
     4261 aaggcgctcc tgccccgcct ccacgcggtg aatgagcgcg aggcctgggc gatggccctg
     4321 aagcccgagg aattccccgc taagacggcc gagctcaagg cccgcgtcgc ctccggcgag
     4381 ccactcgact ccgtattgcc ggaggccttc gcgctggcca gggaggcggc cacggcc
//
LOCUS       DAOOLN010000019         4413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_23863_length_4413_cov_3.526847, whole genome shotgun sequence.
ACCESSION   DAOOLN010000019 DAOOLN010000000
VERSION     DAOOLN010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23863_length_4413_cov_3.526847"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PLW80_00500"
     CDS             <1..712
                     /locus_tag="PLW80_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65008.1"
                     /translation="WQGPGGWAEYNSPDGKSVVDGAVVRVPDPFANVGHPDPAIAGYM
                     FYVYNVQAGLGVWSQYTPGKTGFARPSRQFERDFEGLMGNPIRAAAYAKAAREDTLVN
                     VTIDGLVVTVGKPASKTVHYGHMNKADPRSTYMALSAGSIGYRYNYKATVEFFKKNPT
                     ADYSSAVIKQTKLSLVEDRSVDANAAASEYTAASDGVYFVADAVSGATFSDFQHYALE
                     LQAAYKMALAERAVKFKK"
     gene            complement(793..1686)
                     /locus_tag="PLW80_00505"
     CDS             complement(793..1686)
                     /locus_tag="PLW80_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65009.1"
                     /translation="MMEIRFRFLKLGLGSRLVAAAALYTAAVGLQLILGSPFFGLPAI
                     LAAWLLLALKPATNKPDDQGLEEWRAVTDGEVSRMADALARSRKLRNGVIGPVAAGVM
                     LLGLLLFGTIVLAAVGSRLWLVTLDLALFSLPGLFFGRIGVHVPAELDLAMGRFLAAM
                     SVPLPEGYAMTPYLRFDKDGQGRDVPENLRLMLEPRRKPDDLVGVQFQAAINKGPNGN
                     VPYIYAVALTRGRSGPAYAQLSGLRLRGYEVEPGGDDEYGTVVVRQDTSGGGYHTTDD
                     DCESLVRLMVKALERTEALTL"
     gene            complement(1683..2990)
                     /locus_tag="PLW80_00510"
     CDS             complement(1683..2990)
                     /locus_tag="PLW80_00510"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB65010.1"
                     /translation="MSRAIEELEWVYGFWRGLSPYAYARTEDEVVILPPNLVYKTNRT
                     GAALLEYLGTGGRFEDLPGFDDEKAAQVGSFFLSLKAAYEGRPVELERVRYGFDFTRL
                     PVLGEIAITYRCNNRCLFCYAGCGDSSGLALAQGDGASELDTAEWIRVLDLFKDEAKI
                     PFFSFTGGEPLMRPDVEELAAYAVSRGLRVNLVTNGRLATPERAASLYASGLRTAQVS
                     LEAPEAALHDRLCGAEGAFDESVAGIIAMIAAGISVQTNSTLTRANAEALLGLPAFVA
                     GLGVRRMSMNLFIPTGTGRHGGGLFFPYAEAGAFIDEARRRAHEAGVDFYWYSPMPMC
                     LYNPIARGLGNKSCAACDGLLSVSPSGDVLPCSSWPEPVGNLLSDGFERVWFSEAAAW
                     FKRKRYAPEPCMTCGSFTACQAACPLYWKFAGHDEIKPRAEAR"
     gene            complement(2987..>4413)
                     /locus_tag="PLW80_00515"
     CDS             complement(2987..>4413)
                     /locus_tag="PLW80_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65011.1"
                     /translation="TRCYADLDGGRRVPLRVDDLGSGRYDVVLEGDRRFSGTAYYVMN
                     YAGDFAGAGLVGLTESPERGSLVYFDWAPVEWDEALEYRAVRLVLPVRVSGERLGDDE
                     KRSVPMLTESWVNEQNRIDYYGSQGDDGAYYLTALFYQETPRANAEQRLGLYFPEGYL
                     DLDAALGSRATTDGIGQGERGDRTGGPAATIGLLAALGAAIAAALLLYRRRAAKHAER
                     ASLVEAIAWAGDAWTPPKLFAGSYQVPGKVPEDLHPVEAALLLELALPRIASIMVDAL
                     VAQGLVAVEREDPLKLRFLSERAAADEIEERFMASFDADGNVLPGLLADFFEGAIKSL
                     QEKVWDCDLEATKDHYRKKLLEAGRRADEVASWDESRRTAYYREHRAWMYWRSYAVIS
                     TRPDRLAGVRLPEGMTAGYADFMRSAVCYSGCFTPDNVGTVGSCHSACHNACHDACHS
                     ACHSACHSACHSACHSACVSGSSH"
BASE COUNT          772 a         1487 c         1496 g          658 t
ORIGIN      
        1 gtggcaggga ccgggcggct gggccgagta caactcgccc gatgggaagt cggtcgtaga
       61 cggcgcggtc gtccgcgtgc ccgacccgtt cgccaacgtc ggccatcccg acccggccat
      121 cgccggctac atgttctacg tctacaacgt ccaggccggc ctcggcgtct ggtcccagta
      181 cacccccggc aagaccggct tcgcccgccc ctcgcgccag ttcgagcgcg atttcgaggg
      241 cctgatgggt aacccgatca gggccgccgc ctacgcgaag gccgccaggg aggacacgct
      301 cgtcaacgtg acgatagacg ggctcgtcgt gaccgtcggc aagcccgcct cgaagactgt
      361 gcactacggg cacatgaaca aggccgaccc caggtccacc tacatggccc tgagcgctgg
      421 cagcatcggc taccgctaca actacaaggc gacggtcgag ttcttcaaga agaaccccac
      481 ggccgactat agctccgccg tgatcaagca gaccaagctc agcctcgtcg aggataggag
      541 cgtcgacgcc aacgccgccg cctccgagta cacggccgcg agcgacggcg tctacttcgt
      601 cgccgacgcg gtgtccggcg cgaccttctc ggacttccag cactacgccc tcgaactcca
      661 ggcggcctac aagatggccc tggcagagcg cgcggtcaag tttaagaagt aatatctctg
      721 ggtaaggaac ggccgcccta tcgtataggg cggccgttcc ttttttatgg cgaggtcgga
      781 gacggaggcc cgctataggg tcagggcctc cgtacgctcg agggctttga ccatgagccg
      841 gacgagcgac tcgcagtcgt catcggtcgt atggtagcct ccgccggagg tatcctgcct
      901 gaccacgacg gtgccgtact cgtcgtcgcc gcccggctca acctcatagc cgcggagtcg
      961 cagcccgctc agctgcgcgt aggccggccc tgagcggcca cgagtcaggg cgacggcgta
     1021 tatatagggc acgttgccgt tcggcccctt gttgatggcc gcctggaatt gcacgcccac
     1081 caggtcgtcc ggcttgcgcc tcggctcgag catcaggcgc aggttctcgg gaacgtcacg
     1141 gccctgtccg tccttgtcga agcgtaggta tggcgtcatc gcgtagccct ccggtagcgg
     1201 cacgctcatc gcagccagga aacggcccat cgcaaggtcg agttccgccg gtacgtggac
     1261 gccgatacgc ccgaagaaca gcccgggcaa cgagaacagc gccaggtcga gcgttaccag
     1321 ccacagcctc gagccgacgg cggccagcac gatggttccg aagagaagca ggccgagcag
     1381 catgacgccg gcggctacgg gccctatgac gccgttccgc agcttccgcg accgggccag
     1441 agcgtcggcc atcctggaga cctcgccgtc ggtcacggct cgccactcct ccaggccctg
     1501 gtcgtcgggc ttgttcgtgg ccggcttgag cgccaggaga agccaggcgg cgagtatcgc
     1561 cggcaggccg aagaacggcg agcccaggat caactgaagg ccgaccgccg cggtgtacag
     1621 ggcggcggcc gccacgagcc gggagccgag acccagcttg aggaacctaa aacgtatttc
     1681 catcatcgag cctccgcgcg cggcttgatc tcgtcgtggc cggcgaattt ccagtagagc
     1741 gggcaggcgg cctgacaggc cgtgaacgag ccgcaggtca tacacggctc cggcgcgtag
     1801 cgtttgcgct taaaccaggc cgccgcctcc gagaaccaga cccgctcgaa gccgtccgac
     1861 agcaggttgc caaccggctc gggccacgac gagcacggta gcacgtcgcc ggatggcgag
     1921 acggacagga gaccgtcgca ggcggcgcag cttttattgc cgagccccct ggcgatgggg
     1981 ttgtacaagc acatcggcat cggcgagtac cagtagaagt cgacgccggc ctcgtgggcg
     2041 cgacgcctgg cctcgtcgat gaaggcgccg gcctcggcgt acggaaaaaa gaggccgccg
     2101 ccatggcggc cggtgccggt cggaatgaac aggttcatcg acatgcgacg aacgccgagc
     2161 ccggcgacga aggctggcag cccgaggagc gcctcggcgt tggcccgggt caacgtcgag
     2221 ttggtctgga cggatatgcc ggcggcgatc atggctatga taccggcgac gctttcatcg
     2281 aacgcgcctt cggcgccgca tagtctatcg tgcagggccg cctcgggcgc ctctaggctg
     2341 acctgagcgg tgcggaggcc ggaggcgtac agcgacgcgg cccgttccgg cgtcgcgagg
     2401 cgcccgttcg tgaccaggtt gactctgagt ccgcgcgaga cggcgtaggc agccagctcc
     2461 tcgacgtccg gtctcatgag aggctcaccg ccggtgaacg agaagaacgg aatcttggcc
     2521 tcgtccttga acaggtcgag gacgcgtatc cactccgcgg tatcgagctc cgacgcgccg
     2581 tcgccctgcg cgagggccag gccgctcgag tcgccgcaac cggcgtagca gaacaggcag
     2641 cggttattac aacgataggt gatcgctatc tcgccgagca cgggtagccg cgtgaagtcg
     2701 aagccgtagc gcactcgctc cagctcgacc gggcggccct cgtaggccgc cttcagggac
     2761 aggaagaacg agccgacctg agccgccttc tcgtcgtcga agccgggcaa gtcctcgaag
     2821 cgtccgcccg tgccgaggta ctcgagcagg gcggcgccgg tccggttggt cttatagacc
     2881 aggttgggcg gcaggatgac cacctcgtcc tcggtccgcg cgtaggcgta gggcgatagc
     2941 ccccgccaga agccgtagac ccactcgagt tcttcgatcg cgcggctcaa tgcgaactcc
     3001 ccgaaacgca ggcggaatga caggcggagt ggcaggccga atggcaagcc gagtggcagg
     3061 ccgaatggca ggcgtcgtgg caggcgttat ggcaagccga atggcacgag ccaaccgtcc
     3121 cgacgttgtc gggcgtgaag cagccggagt agcagaccgc cgagcgcatg aagtccgcgt
     3181 agccggccgt catgccctcg ggcaagcgca cgccggccag tcgatccggc ctcgtcgaga
     3241 tgacggcgta gctacgccag tacatccagg cgcggtgctc gcgatagtag gccgtgcgac
     3301 gggactcgtc ccagctcgcg acctcgtcgg cccgcctgcc ggcctcgagc agcttcttgc
     3361 ggtaatgatc cttcgtcgcc tccaggtcgc agtcccagac cttctcctgg aggctcttga
     3421 tggcgccctc gaagaagtcg gcgaggagcc ccggcaggac gttcccgtcg gcgtcgaagg
     3481 aggccatgaa acgctcctcg atctcgtcgg cggccgcgcg ctcagatagg aagcgcagct
     3541 tgagcgggtc ctcccgctcg acggccacga ggccctgggc gacgagggcg tcgaccatga
     3601 tcgaggcgat gcgcggaagg gccagctcga ggaggagcgc agcctcgacc ggatgcagat
     3661 cctccggtac cttgcccggt acctggtagc tgccggcgaa cagcttgggc ggcgtccatg
     3721 catcgccggc ccaagcgatg gcctcgacca gagaggcgcg ctcggcgtgc ttggccgcgc
     3781 gccggcgata gaggagcagg gccgcggcga tcgccgcgcc gagggccgcg agaaggccga
     3841 tggtcgcggc gggtccgccg gtccgatcgc cccgctctcc ctggccgatg ccgtcggtcg
     3901 tcgcgcggct accgagcgcc gcgtcgaggt caaggtagcc ctcggggaaa tagaggccaa
     3961 ggcgctgctc cgcgttggcc cgaggcgtct cctgatagaa gagcgccgtc aggtagtagg
     4021 ccccgtcgtc accctgggag ccgtagtagt cgatgcggtt ctgctcgttg acccatgact
     4081 cggtgagcat cggcacggag cgcttctcgt cgtcccccaa gcgctcccca gagacgcgga
     4141 cgggcagcac gagcctgacg gcccggtatt cgagcgcctc gtcccattcg accggagccc
     4201 agtcgaagta gaccagggag ccgcgctccg ggctctcggt caggccgacg agcccggccc
     4261 cggcaaagtc accggcgtag ttcatcacgt agtaggccgt gcccgagaac ctgcggtcgc
     4321 cctcgagcac gacgtcgtag cgtcccgagc ccaggtcatc gacgcgtagc gggacgcgcc
     4381 gcccgccgtc gaggtcggcg tagcagcgcg tca
//
LOCUS       DAOOLN010000020         4338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_24396_length_4338_cov_3.028251, whole genome shotgun sequence.
ACCESSION   DAOOLN010000020 DAOOLN010000000
VERSION     DAOOLN010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24396_length_4338_cov_3.028251"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLW80_00520"
     CDS             <1..237
                     /locus_tag="PLW80_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65012.1"
                     /translation="EKAGEGGKGLLEVFGPGAKPVIPADCLGALGPAVEAAAAKTAAI
                     IERGSIYVPAVRDRDDVCEGCELRPVCRARYAVR"
     gene            240..3713
                     /locus_tag="PLW80_00525"
     CDS             240..3713
                     /locus_tag="PLW80_00525"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPB65013.1"
                     /translation="MGAWNDIKAGLDPARQLPAVEETRSAVVSAGAGSGKTRVLAVRY
                     LHLVKERGIPPERILCMTFTKKAAAEMSERIRAMLAACAVDDDDFKKALEAFPSSRVS
                     TLDSFCSEVARDGCARWGVPPDFAVDKDPGDLGALAIEFLLERRDDPVAASFIAASGF
                     EKAAEALAALAGGREGLIATDRPFDPDSQAAALGALLADAHGQALELLRGGLALDDAG
                     VKGAMAWLEAAGAFAPEPPAANDAEALGAAAAAYRALAGLRLSTGKAEAAVYYNEAGK
                     RVRPLLSMAALACDALADGRRSRALDLLREFVALAADARAASGSLTFADVAAIALRTL
                     ETDLTLRDWYKSRYDAIMVDEFQDDNELQKRVLYCLAERRDRTDPGRGPAVGPGDLEP
                     GVLFFVGDEKQSIYKFRGADVTVFRGLAAELASAPGGSGNHGLDVNWRSEPGLIDFFN
                     ATFARVMPAPDDASAQPFEARFSSLGSGPATPGVEPTVAYLESYEPEDGDYLPSSEAE
                     AWRIAELARSLVDDGRIVAAKGPDGRKVARPCRWEDIAILFRSTASQNVVERYLRLLG
                     VPYTAASTAGLYAESILGDLYAMLRLAVYPDDRLACAAVLRGPFARLSDDSVFSLLSD
                     GEGCALDRDGDGLPPDDRARLAAARETWRGLRSMADREPLRALVEYLWYGRGLRWNAL
                     KDLQTTAFLEHFDYAWSLAAAADARGDRLVDLVSSLEATIGRLDRFDENVSKESARGV
                     SLMTVHLSKGLEFPVVILPDLDATGRNGLRSPVEPSGRFGSSMRLLGRDDDPADPIAE
                     LCSALRKHERGEGGEAMDEDLAETTRLFYVACTRAVSRLYLVGKVPRSADASGRSFRG
                     LFIRAWPWAGPASLGDEEFLSDRPGDAPPYLGVEYVPPRTKLDYVGLVGSEAADQARG
                     GGSGASRMAASAAAAVRAVPRRKGRWAVTQAAERIAEKSPRVQSAAPPVDAAAEAAEA
                     AESAEGSLRETEFGTLCHQFVEALLARPGEPPEPSGPVAVSLARLPKGRADLVRAEAL
                     ALAEGFVRSERGRAAIAARSAKASEGTVFEMEYPFVWRAAAGQAGAFPIALTGSIDLV
                     YGDRTGLWVVDFKTDRVVAPSRHAFQLAVYRDAAEAMFGRPARAVVHYLRHGEEREIV
                     ESPDLSLLEGE"
     gene            3952..>4338
                     /locus_tag="PLW80_00530"
     CDS             3952..>4338
                     /locus_tag="PLW80_00530"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPB65014.1"
                     /translation="MNDTSPPVKRPGFFSIFGFFFGISAITIGGGYVMVPVIQRALEK
                     RGWLPEDEFYDLFATAQSVPGPLALNTAMFAGRKVAGLPGFLAAFLGIMLPPFAAILL
                     LATLLGVIGDWPVVQGFLDGAFAVVPG"
BASE COUNT          638 a         1539 c         1485 g          676 t
ORIGIN      
        1 gaaaaggccg gcgagggcgg caaggggctg ctcgaggtct tcggcccggg tgccaaaccg
       61 gtcatccccg ccgactgcct cggtgcgctc gggccggccg tggaagcggc ggccgccaag
      121 acagcggcca tcatcgagcg gggctcgatc tacgtcccgg ccgtgcgcga ccgggacgac
      181 gtctgcgaag ggtgcgagct gcggccggtc tgccgcgccc gctacgcggt gaggtaggca
      241 tgggagcctg gaacgatatc aaggcggggc tcgaccccgc gcgccagcta ccggcggtcg
      301 aggagacccg atcagccgtc gtctcggccg gcgccggctc cggcaagacg cgcgtgctcg
      361 cggtgcgcta cctgcatctg gtcaaggagc gcgggatccc gccggagcgc atactctgca
      421 tgaccttcac caagaaggcg gccgcggaga tgagcgagcg catccgcgcg atgctggccg
      481 cctgcgcggt cgacgacgac gatttcaaga aggcgctcga ggccttcccc tcgtcgcgcg
      541 tgtcgacgct cgactcgttc tgctccgagg tggcgcgcga cggctgcgcc cgctggggcg
      601 tgccgccgga cttcgcggtg gacaaggatc ccggcgacct aggcgctctg gcgatagaat
      661 tcctactcga gcggcgcgac gatccggtcg cggcctcgtt cattgcggct tccggcttcg
      721 agaaggccgc cgaggcgctc gccgccctgg cgggaggccg cgaggggctc atcgccacgg
      781 accggccctt cgaccccgat agccaagcgg cggccctcgg cgccctcctg gctgacgccc
      841 acggtcaggc gctcgagctc ttgcgcggcg ggctcgctct ggacgacgcc ggggtcaagg
      901 gagcgatggc ctggctcgag gcggcggggg ccttcgcgcc cgagccgccg gccgcgaacg
      961 acgccgaggc gctcggggct gccgcagcag cctatcgcgc gctcgcgggg ctacgcctct
     1021 cgaccggcaa ggccgaggcg gccgtctact acaacgaggc gggcaagcgc gtccgcccac
     1081 tcctttcgat ggccgccctg gcctgcgacg cgctggccga cggccgcagg agccgcgccc
     1141 tggacctgct acgcgaattc gtcgccctgg ccgccgacgc ccgggcggcc tcgggctcgc
     1201 tgaccttcgc ggatgtcgcc gccatagcgc tacgtaccct cgagaccgac ctgacgctac
     1261 gcgactggta caagtcgcgc tacgacgcga tcatggtcga cgagttccag gacgacaacg
     1321 agctccagaa gcgggtgctg tactgcctgg ccgagcgccg cgaccgtacc gaccccgggc
     1381 gtggcccggc cgtcggcccc ggagatcttg agccgggcgt gctgttcttt gtcggtgacg
     1441 agaagcagag catctacaaa ttccgcggcg ccgatgtgac ggtgttccgc ggcttggccg
     1501 cagagctggc ctcggcgccc ggcggctcag gcaaccacgg cctggacgtc aactggcgct
     1561 ccgagcccgg cctgatcgac ttcttcaacg ccaccttcgc gcgggtcatg cccgcccccg
     1621 atgacgcgtc cgcccagccc ttcgaggcca ggttctcatc gctcggctcc ggtccggcaa
     1681 cgccaggcgt cgagccgacg gtggcctacc tcgaatcgta cgagcccgag gatggggatt
     1741 acctgccttc gagcgaggcc gaggcctggc gcatcgccga gctggctcgc tcgctcgtcg
     1801 acgatggccg gatcgtcgcg gccaagggcc ccgatggccg caaagtcgcc cggccctgcc
     1861 gctgggagga catagccatc ctgttccgct cgacggccag ccagaacgtc gtcgagcgat
     1921 acctgcgctt gctcggcgtc ccctatacgg ccgcgtcgac cgcgggcctc tacgccgagt
     1981 cgattcttgg cgacctgtac gcgatgctca ggctggcggt ctacccggac gaccgcctgg
     2041 cctgcgccgc cgtgctccgc ggccccttcg cgaggctctc ggacgattcg gtcttctcgc
     2101 tgttgtccga cggcgaaggc tgcgcgctgg accgcgacgg cgacggctta ccgccggacg
     2161 atcgcgccag gctggccgcc gcccgagaga cctggcgggg cctgcgctcc atggccgacc
     2221 gcgagccgct gcgcgccctc gtcgagtacc tatggtacgg acggggcttg cgctggaatg
     2281 cgctcaagga tctccagacg acggccttcc tcgagcattt cgactacgcc tggagcctgg
     2341 ccgccgcggc cgacgcgcgc ggcgatcggc tggtcgacct cgtatcgtcg ctcgaagcga
     2401 cgataggccg gcttgaccgc ttcgacgaga acgtttcgaa ggaaagcgct cgaggcgtat
     2461 cgctgatgac cgtccacctg agcaaggggc tcgaattccc cgtcgttata ctgcctgacc
     2521 tcgacgcgac cggtcggaac ggcctccgct cgccggtgga gccctcgggc cgcttcgggt
     2581 cgtcgatgcg cctcctcggg cgggacgacg atccggcgga tccgatagcc gagctatgct
     2641 cggctctgcg caagcacgag cgcggcgaag gcggcgaggc tatggacgag gacctggccg
     2701 agacgacgag gctgttctac gtcgcctgta ccagggccgt ttcaaggctc tatctggtcg
     2761 ggaaggtacc gcggagcgcc gacgcctcgg gccggagctt ccgcgggctg ttcatacgcg
     2821 cctggccctg ggccggcccg gctagcctgg gcgacgagga gttcctatcg gatcgcccgg
     2881 gcgatgctcc gccctatctc ggggtcgagt acgtaccgcc gcggaccaag ctcgactacg
     2941 tcgggctcgt cggtagcgag gccgcggacc aagcgcgagg cggtgggtcc ggggcgtcgc
     3001 gcatggcggc ttcggccgcc gccgccgtcc gggccgtgcc gaggcgaaag ggccgctggg
     3061 cggtcaccca ggcggccgag cggatagcgg aaaagtcgcc gagggtccag agcgcggcac
     3121 cgcctgtcga cgccgccgcc gaggccgccg aggccgccga gtccgccgag ggctcgctac
     3181 gggagacgga gttcggcacg ctctgccacc agttcgtcga ggcgctccta gctcggccgg
     3241 gagaaccgcc cgagccttcc ggcccggtgg ccgtctcgct cgcccgcctg cccaagggtc
     3301 gcgccgacct cgtccgcgca gaggcgctcg cgctggccga aggcttcgtg cgatcggagc
     3361 gggggcgggc cgcgatcgcc gcgcggtcgg ccaaagcctc ggaaggaacc gtcttcgaga
     3421 tggagtaccc cttcgtctgg cgggccgccg caggtcaggc cggggccttc cccatcgcgc
     3481 tgaccggtag catcgacctc gtctacggag accggacagg tctttgggtc gtcgacttca
     3541 agaccgacag ggtcgtcgcc ccgtcgcgcc acgccttcca gctagccgtg taccgcgacg
     3601 cggccgaggc catgttcggc cgcccggcga gagcggtcgt ccactaccta cgccatggcg
     3661 aggaacgcga aatcgtcgaa tcgccggatc tatcgctcct cgagggcgag taatgttttt
     3721 ttatccagtt gctccaaata atcaaaaaaa tactagacta agaatggatt atctgccata
     3781 ctgctggaac gaagaatggc acgcggcact acaccttcgt tacgccgcgc atccagcccg
     3841 atgtctgtac ccaacacggc cccgcatcca gcgtgccgtt cttcgtgcac taaaacatag
     3901 caataaattg agcctaggca cggcgccagc cgccgcgcga aaggtacgcc cgtgaacgac
     3961 acctcaccgc cggtcaagcg accgggcttc ttctccatct tcggcttctt cttcggtatc
     4021 tcggccatta ccataggcgg cggctacgtc atggtgccgg tcatccagcg agccctcgag
     4081 aagcgcggct ggctaccgga ggacgagttc tacgacctgt tcgcgaccgc ccagagcgtg
     4141 cccggccccc tggcgctgaa caccgccatg ttcgccgggc gcaaggtcgc cggactaccc
     4201 ggcttcctgg ccgccttcct cggcatcatg ctaccgccgt tcgccgccat actgctgctc
     4261 gccaccttgc tcggcgtgat cggggattgg ccggtcgtac agggcttcct ggacggcgct
     4321 ttcgcggtcg taccgggc
//
LOCUS       DAOOLN010000021         4306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_24630_length_4306_cov_3.573277, whole genome shotgun sequence.
ACCESSION   DAOOLN010000021 DAOOLN010000000
VERSION     DAOOLN010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24630_length_4306_cov_3.573277"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..486
                     /locus_tag="PLW80_00535"
     CDS             166..486
                     /locus_tag="PLW80_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trp family transcriptional regulator"
                     /protein_id="HPB65015.1"
                     /translation="MNDETGLVSTNLTEMSKILAATADAGLIEDFMYSLFTQAEADEI
                     AKRWALVKRIAEGVPQRAIAAELGLSLCKITRGSRELKKENSSFRRMLELGGYEVVEK
                     KRKA"
     gene            complement(579..1082)
                     /locus_tag="PLW80_00540"
     CDS             complement(579..1082)
                     /locus_tag="PLW80_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPB65016.1"
                     /translation="MANPIVTFKTSLGSFDIELFEDKSPVTVKNFTEYVKAGFYDGTV
                     FHRVIPGFMVQGGGMTPGLKEKDTNAPIKNEADNGVGNERGTIAMARTSDPHSATCQF
                     FINLVDNKPLNFRSKDQAGWGYCAFGKVTSGMDVVDAIAKVETGNRFPHQNVPKTDVL
                     IESASLA"
     gene            1208..1894
                     /gene="pyrH"
                     /locus_tag="PLW80_00545"
     CDS             1208..1894
                     /gene="pyrH"
                     /locus_tag="PLW80_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPB65017.1"
                     /translation="MTKVLSLGGSIVAPDVPDAAFVKAFALTARRWLDADAARRLIVV
                     VGGGGPARAWQKAYRDAADAPDADAQDWIGIMATRLNAQLVRSLFLDLCPHEVVTDPT
                     SVAVFGGRVLVAAGWKPGFSTDFDAVVLAERFQADVVVNLSNIAKVYTADPRTDPNAK
                     PIDDISWKDFRAMVGDEWTPGKNVPFDPVASRRAEQLGLRVVCAAGKDLANLEAILDG
                     KPFVGTTIGA"
     gene            complement(1905..2948)
                     /locus_tag="PLW80_00550"
     CDS             complement(1905..2948)
                     /locus_tag="PLW80_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB65018.1"
                     /translation="MTTKGVSGATRRALQATIYHLPGAPYGAPRPVARRYFGRRSRAL
                     ALVAAATLASGWLGSCSSAPKRQTAVVERKNEAAEYAALADGFLASGQYSSALQYYGE
                     ALDASLSVDDVAGAVKSRGSLGRAYLAIGGLDDAAREFGDALEDARALGEPALVALCL
                     SNLGELRYASGDWDAADALFAEAEALAAGDEAIAAVVAHNRGVVAMARADYAAAEAYV
                     LRSAAANEKARRWSELGSNRYALASIANARGDLPGALAWAAKALEADKRAENSPGIGA
                     DLEALARLQLKSGNGAAAFDYYRRAFGLWLSLGRADDAERCLAALSGLASSLGKDGYA
                     ARYEALLARLRER"
     gene            complement(2945..4042)
                     /locus_tag="PLW80_00555"
     CDS             complement(2945..4042)
                     /locus_tag="PLW80_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPB65019.1"
                     /translation="MKFKIKFADQIVGLFIIVALAFFAVIVVLIGANQRWFAKDYRFV
                     TSFPSAAGVAPGTSILMKGFTIGKIDKLRLNADNAVDADFHIYDTYYDKVREYSILEL
                     TVSPIGLGSQLLFHPGTGEARLEEGAFVPTADSIQGQSLIDQELVVIPPKDDTITRLL
                     ANVNPLLENTNKTIVTVNRTLTEVNRALAGQSSGPLGDIVGDVSVAAAALPGAMAGVG
                     GIVGDVESRVAILLDQAGSLLAELETIAGNLAATTEAVRDPTGLVTTLLDPSGSIKTF
                     LDDDDALYKKVMSIVGEADKAVRSLQGIITGLNGEMPKIAGLLNETKAAIKQAQDVLE
                     GVKNNPLIRGGVTERVEQESLYSAMREGSFE"
     gene            complement(4046..>4306)
                     /locus_tag="PLW80_00560"
     CDS             complement(4046..>4306)
                     /locus_tag="PLW80_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65020.1"
                     /translation="RRRTVVIVANSSELAYRFADRLGIIKGGRLVAFGAYEETVGAAE
                     AALSGSIARLKARGERVAKPGRERGPAPGLDAGSENSQAGDV"
BASE COUNT          678 a         1399 c         1492 g          737 t
ORIGIN      
        1 ggcgaccctg cgccagatgc gaggaaatgc tatgaacacc acttatgttt caaataacaa
       61 aacgtcgata taaatgttga aacaattttg gctaaaaatg cataaaaact ggtgacaaga
      121 ctaatctgag tgattatact gttcctacat ggagaatatg cgagcatgaa cgacgaaacc
      181 ggattggtgt cgaccaacct gaccgagatg tccaagatcc tcgccgccac ggcggacgcc
      241 ggactcatag aggattttat gtacagcctt tttacccagg ccgaggccga cgagatagcc
      301 aagcgctggg cgctggtcaa gcgcatcgcc gagggcgtac cccagcgggc catcgccgcg
      361 gagctgggcc tgtcgctctg caagatcacc cgcggatcga gggagctcaa gaaggagaat
      421 tcttcgttcc ggcgcatgct cgagcttggc gggtatgagg tcgtggagaa gaagcggaag
      481 gcctagggac gactgcctaa ggatgacgga ggccgctcgg ctatggcccg ggcggccttc
      541 atcgtctcgg ggcccgacgc gggtcccatc gcggggcctt aggcgaggct ggcgctctcg
      601 atgagcacgt ccgtcttggg cacgttctgg tgcgggaagc ggttgcccgt ctcgaccttg
      661 gctatggcgt cgaccacgtc catgccgctc gtcaccttgc cgaaggcgca gtagccccag
      721 ccggcctggt ccttggacct gaagttgagc ggcttgttgt cgaccaggtt gatgaagaac
      781 tggcaggtgg cgctatgcgg gtccgaggta cgggccatcg cgatggtgcc gcgctcgttg
      841 cccacgccgt tgtcggcctc gttcttgatc ggcgcgttcg tatccttctc cttgaggccg
      901 ggcgtcatgc cgccgccctg caccatgaag ccggggatga cccgatggaa cacggtgccg
      961 tcgtagaagc cggccttgac gtactcggtg aagttcttga ccgtgacggg gctcttgtcc
     1021 tcgaagagct cgatatcgaa cgagccgagg ctcgtcttga aggtgactat cgggttggcc
     1081 atgtcggcct cctttcgcgt agggtcagag cctaacccga tacggaggcg ataggcaagg
     1141 tcggccggac ctcatggtga cgaccgcggc cgtttggggt actatctatc catagtggag
     1201 gacggcgatg accaaggtac tatctctcgg cggctctatc gtggctcccg acgttccgga
     1261 cgcggccttc gtcaaggcgt tcgcgctgac ggccaggcgc tggctggacg ccgacgcggc
     1321 gcgcaggctg atcgtcgtgg tcggcggcgg cggcccggcc cgggcgtggc agaaggccta
     1381 ccgcgacgcc gcggacgcgc cggacgccga cgcccaggac tggataggca tcatggccac
     1441 gagattgaat gcccagctgg tgcgcagcct gttcctggac ctctgtcccc atgaggtggt
     1501 gaccgacccg acgagcgtcg ccgtgttcgg cggcagggta ctcgtggcgg ccggctggaa
     1561 acctggcttc tccacggact tcgatgcggt ggtgctggcc gagcgcttcc aggccgacgt
     1621 ggtggtcaac ctgtcgaaca tcgccaaggt ctacacggcc gacccgagga ctgacccgaa
     1681 cgccaagccg atagacgaca tctcctggaa ggatttccgc gcgatggtcg gcgacgagtg
     1741 gacgccgggc aagaacgtgc ccttcgaccc ggtcgcctcg aggcgggccg aacagctcgg
     1801 cctgcgcgtg gtctgcgccg ccggcaagga cctggccaac ctggaggcca ttctggacgg
     1861 caagcccttc gtcggcacga cgataggcgc ctgacgtcgg gcggctagcg ctcgcggagc
     1921 cgagccagca gggcctcgta gcgggcggcg tagccgtcct tgccgagcga cgaggcgagg
     1981 ccgctcaatg cggccaggca gcgctccgcg tcgtcggcgc ggccgagcga cagccagagt
     2041 ccgaaggccc gacggtagta atcgaaggcc gccgcgccgt tccccgactt gagctggagc
     2101 ctggcgagcg cctccaggtc ggccccgatg cccggcgagt tctccgccct cttgtccgcc
     2161 tcgagggcct tggcggccca ggcgagcgcg cccggcaggt cgccgcgagc gttggctatc
     2221 gaggcgagcg cgtagcggtt cgagccgagc tccgaccagc gccgcgcctt ctcgttggcg
     2281 gcggccgagc gcaacacgta ggcctcggct gccgcatagt cggcgcgggc catcgcgacg
     2341 acgccgcggt tgtgcgcgac cacggccgct atcgcctcgt ctccggcagc cagcgcctcg
     2401 gcctcggcga agagcgcgtc ggccgcgtcc cagtcgccgc tcgcgtagcg cagctccccg
     2461 aggttcgaca ggcacagcgc gacgagggcc ggctcgccga gggcgcgcgc gtcctcgagc
     2521 gcgtcgccga actcacgggc cgcgtcgtcg aggccgccga tggccaggta ggcgcggccg
     2581 agcgacccgc gggacttgac cgctcccgcg acatcgtcga ccgacaggct ggcgtcgagc
     2641 gcctctccgt agtactgcag cgccgacgag tactggcccg aggccaggaa gccgtcggcc
     2701 agggcggcgt actcggccgc ctcgttcttg cgctcgacga cggccgtctg ccgcttgggc
     2761 gcgctcgagc aggagccaag ccagcctgag gccagggtcg cggcggctac gagcgccagg
     2821 gcccgcgacc gtcgcccgaa gtaccggcgc gccaccgggc gcggcgcgcc gtagggcgcg
     2881 cctggaagat ggtatatcgt cgcctgaagc gcccgtctcg tcgcgcccga tacgcccttc
     2941 gtcgtcattc gaagctcccc tcgcgcatcg cgctgtacag cgattcctgc tcgacccgct
     3001 cggtgacccc gccgcggatc agcgggttgt tcttgacgcc ctcgagcacg tcctgggcct
     3061 gcttgatcgc ggccttggtc tcgttgagga ggccggcgat cttgggcatc tcgccgttca
     3121 gcccggtgat gatgccctgg aggctacgca ccgccttgtc ggcctcgccg acgatgctca
     3181 tcaccttctt gtagagcgcg tcatcgtcgt ccaggaaggt cttgatggag ccggaggggt
     3241 cgagcagggt ggtcacgagg ccggtcgggt cgcggaccgc ctccgtggtg gccgcgaggt
     3301 tgccggctat ggtctcgagc tcggcgagca gggagccggc ctggtccagg agtatcgcga
     3361 cccgggactc gacgtcgccg acgatgccgc cgacgccggc catcgcgccg ggcagggcgg
     3421 ccgcggcgac gctgacgtcg ccgacgatgt cgccgagcgg gccggagctc tgcccggcga
     3481 gggcccggtt gacctcggtc agggtgcggt tgacggtgac gatggtcttg ttggtgttct
     3541 cgaggagcgg gttcacgttg gccaggagcc gggtgatcgt gtcgtccttg ggcggtatga
     3601 cgaccaactc ctggtcgatc agcgactggc cctggatcga gtcggccgtc ggcacgaagg
     3661 cgccctcctc gagccgggcc tcgcccgtgc ctgggtggaa caggagctgg gagccgaggc
     3721 cgatcgggga caccgtcagc tcgagtatcg aatactcgcg taccttgtcg tagtaggtgt
     3781 cgtagatatg gaagtcggcg tccaccgcgt tgtcggcgtt cagcctcagc ttgtcgatct
     3841 tgcctatcgt gaagcccttc atgagtatcg acgtgcccgg agccacgccg gcggctgacg
     3901 ggaaggaggt cacgaagcgg tagtctttgg cgaaccagcg ctggttggcg ccgatcagta
     3961 cgacgatgac cgcgaagaag gccagcgcga cgatgatgaa caggcctacg atctggtcgg
     4021 cgaacttgat cttgaatttc atcgtttaca cgtcccccgc ttgagaattc tccgatccgg
     4081 cgtccaggcc cggcgccggc ccgcgttccc gtcccggctt cgccacgcgc tcgcctcgcg
     4141 ccttgagcct ggctatcgag cccgatagtg ccgcctccgc ggcgccgacc gtctcctcgt
     4201 aggcgccgaa ggctaccagc ctgccgccct tgatgatacc gagccggtcg gcgaagcggt
     4261 aggccagttc ggagctgttg gccacgatca cgaccgtgcg ccgccg
//
LOCUS       DAOOLN010000022         4263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_24964_length_4263_cov_3.273764, whole genome shotgun sequence.
ACCESSION   DAOOLN010000022 DAOOLN010000000
VERSION     DAOOLN010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24964_length_4263_cov_3.273764"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..1436
                     /locus_tag="PLW80_00565"
     CDS             33..1436
                     /locus_tag="PLW80_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPB65021.1"
                     /translation="MTTRAITRPATLAAVSMACLLACLTAGATPAWGQTVAAITQIDG
                     ASLLARQRVRLYVAGIDDGADAVRVQESADGRNWEDRPVEAVTEAANADEGVTFFFLL
                     DDSGSMWPEAGVDEAGSRIGQAKDAARRFIARLGPADRLGLAVFNTRYWRAAEPTADR
                     AAVSAAIDEIARPSRDDAFTELYQSIDRAVLELAAYPGRKAIVVLSDGEDYPYAARTG
                     SPSPEFGEAEVTPDEAAERAIAEGVTCYAVRFGPDRDPDIGSMAERTGGAAFDAADGL
                     ELAEVYATIRNDLLREAAVDYRAGMAPGDRRYVRVSLDDGGNWSAPRYYYAGAVFGQA
                     RSAPGWLDLVPLLLAAAVWLAFWLLKLERPMDRAGLSLLYGPRDARTEVYALSGAKTV
                     IGGSADADITLAGNPAMAADHATILFDELRGEYTIAADAELRVNNRPVKRKRLEPGDV
                     IDMAGTVVVFDEPTKGK"
     gene            complement(1462..3582)
                     /gene="recJ"
                     /locus_tag="PLW80_00570"
     CDS             complement(1462..3582)
                     /gene="recJ"
                     /locus_tag="PLW80_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPB65022.1"
                     /translation="MRWKKRDVSPQAIRELAERFGLDLLSASVLARRGVLSPEEVLYY
                     LEDDLRFQRNPFLFSQMEDAVDRILLAADEGEKVLVFGDRDADGVSATTLMMEALASL
                     GVDARYRLPTEDEKYGLSRAAVDEFAADYGSLIVTVDCGISNHAEIAYAAEKGVDVIV
                     VDHHVLQADEPPPALAVIDPKLESSGYPFRDLSGCGVAYKLAWALRFAKSGLYKQQVA
                     LLNVRPVKDAYVIEALRLSNLVETGRVVETIVPGMVELERTRLVPFLKDRQIFVWDGE
                     IQKRLLAKALGKAAEVNFYDVRPDVARVIPQAAGASLVRLAEMSKVGKYSDRPSGELD
                     AFANLFVSFALRKAGCQGESDADALQLVALSTIADLMPLRDENRLLVRQGLAALNRKP
                     RKGLAELVARQNLTGKRVGSTDAAWQLTPVINAAGRMGKPEVATRMLLSDDAAERGAQ
                     ADALLELNQERRRLGAECWDAVYPAAREAAEASGGKYVIIGRDDLFRGITGIVASRLA
                     DTFKAPAVAGTFMADGTAVASVRSARGFNVKGLLEYCSELFYDYGGHDAAAGFSLPVD
                     KWPDFERMAAEYLSAVDLEEVEETVEIDAELPHEYLAPSLAGLVERMEPFGEGNPALV
                     FMARSVAILQVDIVGKQEKSHLKLLLDFGAHRWPALWWNAAERYGKDFDESDRLDLAF
                     RLQKNYWNGQETPQLVIVDARKAE"
     gene            complement(3579..>4263)
                     /locus_tag="PLW80_00575"
     CDS             complement(3579..>4263)
                     /locus_tag="PLW80_00575"
                     /inference="COORDINATES: protein motif:HMM:NF019448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HPB65023.1"
                     /translation="GQAMGKRIVGVITDMTEPLGDKVGNLLEVEESIDCLEGRGPADL
                     MEVTYRLAAWMLVAAGNDAAGNDAAGNGAAGVAQDVEAATRLCEKAVASGKALELFMR
                     NVRTQGGDVDRMMKMRGSFRSRNVRELRAPAAGYVRSIDAYKIGLAGVYLGVGRNKTS
                     DPVAPDVGFVFERRKGAAVAKGDLVATVYGKDEASLDAAWPLVAEALSVGPEAPAASP
                     LVIKEITAL"
BASE COUNT          662 a         1442 c         1489 g          670 t
ORIGIN      
        1 ggatgaagcg agccgacgat aaggagcgaa ccatgactac gcgagcgatt acccgaccag
       61 cgacgctggc ggccgtctcg atggcctgtc tattggcgtg cctaacggct ggcgcgacgc
      121 ccgcctgggg ccagaccgta gccgcgataa cccagataga cggcgcgagt ctcctcgcca
      181 ggcagcgcgt caggctatac gtcgccggga tcgacgacgg ggccgacgcg gtccgcgtac
      241 aggagtccgc cgacggccgg aactgggagg accggcccgt cgaggcggtg accgaggcgg
      301 cgaacgccga cgagggcgtc acgttctttt tcctgctgga cgactcggga tcgatgtggc
      361 ccgaggctgg ggtcgacgag gccggctcgc gcatcggcca ggccaaggac gcggccaggc
      421 gcttcatcgc gaggctcggg ccggccgaca ggctgggctt agccgtgttc aacaccaggt
      481 attggcgcgc ggccgagccg acggctgacc gtgccgccgt atcggcagcc atcgacgaga
      541 tagccagacc gtcgcgcgac gacgcgttca ccgagctgta ccaatcgata gaccgcgccg
      601 tcctcgagtt ggccgcctac ccgggccgca aggccatcgt cgtcctgtcg gacggcgagg
      661 actaccccta cgcggcccgg accggcagcc cgagccccga gttcggcgaa gcggaggtta
      721 cgcccgacga ggcggcggag cgagccatag ccgagggcgt cacctgttac gccgtgcgct
      781 tcggccccga ccgcgatccg gatataggct ccatggctga acggaccggc ggcgcggcct
      841 tcgacgcggc cgacgggctg gagctggcgg aggtctacgc gacgatccgt aacgacctac
      901 tcagggaggc ggccgtggac taccgggccg gtatggcgcc tggcgaccgc cgctatgtgc
      961 gcgtcagcct cgacgatggc gggaactgga gcgcgccgcg ctactactac gccggcgccg
     1021 tgttcgggca ggcacgttcg gcgcccggct ggctcgacct ggtgccgctg ctcctcgcgg
     1081 cggccgtctg gttggccttc tggctactca agttggagcg tcccatggac cgggctggcc
     1141 tcagcctcct ctacgggccg cgcgatgcca ggaccgaggt ctatgcgcta tcgggcgcca
     1201 agacggtgat cggcggctcg gccgatgccg acatcaccct ggcgggcaat cccgcgatgg
     1261 ccgccgacca cgcgaccatc ctgttcgacg agcttcgcgg cgagtacacc atcgccgccg
     1321 acgccgagct acgcgtcaac aaccggccgg tcaagcgcaa gcggctggaa ccgggcgacg
     1381 tgatcgacat ggcggggacg gtggtggtct tcgacgagcc gaccaaggga aaatgaggcg
     1441 cgggcgcctg gaccgagccg cttactcggc cttcctggcg tcaacgataa cgagctgcgg
     1501 ggtttcctgg ccgttccagt agttcttctg gaggcggaag gccaggtcga gccggtccga
     1561 ctcgtcgaag tcctttccgt aacgctcggc ggcgttccac cagagcgccg gccatcggtg
     1621 ggcgccgaag tcgaggagca gcttgagatg gctcttttcc tgcttgccga ctatgtcgac
     1681 ttgcaggatg gccaccgacc tggccatgaa tacgagggcc gggttgccct cgccgaacgg
     1741 ctccatgcgc tcgacgagcc cggccaacga cggcgccagg tactcgtgcg gcagctcggc
     1801 gtctatttcg acggtctcct cgacctcctc gaggtcgacc gccgacaggt actcggcggc
     1861 catacgctcg aaatccggcc acttgtctac cggcaggctg aagcccgcgg ccgcgtcgtg
     1921 gccgccgtag tcgtagaaca gctcggagca gtactcgagg aggcccttca cgttgaagcc
     1981 gcgggcgctg cgcaccgagg ccacggccgt gccgtcggcc atgaaggtac cggcgaccgc
     2041 cggcgccttg aaggtgtcgg ccaggcggct ggcgacgatg ccggtgatgc cgcggaagag
     2101 gtcgtctcgg ccgatgatca cgtacttgcc gccggacgcc tctgcggcct cgcgggcggc
     2161 cgggtagacg gcgtcccagc actcggcgcc gagccttcgc cgctcctggt tcaattcgag
     2221 cagcgcgtcg gcctgggccc ctcgctcggc cgcgtcgtcg gacagcagca tcctcgtagc
     2281 cacctccggc ttgcccatgc gcccggccgc attgatcacg ggagtcagct gccaggcggc
     2341 gtcggtcgag ccgacgcgct taccggtcag gttctgcctg gcgacgagct cggccaagcc
     2401 cttccgcggt ttgcggttca gggcggccag tccctggcgc accaggagcc ggttctcgtc
     2461 gcgcagaggc atcaggtcgg ctatcgtcga cagggcgacg agctgcaggg cgtcggcgtc
     2521 gctttcgcct tggcagccgg ccttgcgtag cgcgaacgat acgaagagat tcgcgaaggc
     2581 gtcgagctcg ccagacggcc ggtcggagta cttgccgacc ttggacatct cggccaggcg
     2641 gacgaggcta gccccggcgg cctgcggtat gacgcgagcg acgtcgggcc tgacgtcgta
     2701 gaagttaacc tcggccgcct tgccgagggc cttggccagg aggcgcttct ggatttcccc
     2761 gtcccagacg aagatctgcc ggtccttgag gaagggcacg agcctcgtgc gctccagctc
     2821 gaccatgccc ggcacgatgg tctcgaccac gcggccggtt tccaccaggt tggacaggcg
     2881 gagcgcctcg atcacgtagg cgtccttgac cggcctgacg ttgaggaggg cgacctgctg
     2941 cttatagagg ccgctcttgg cgaaacgcag ggcccaggcc agcttatagg ctacgccgca
     3001 acccgacagg tcgcggaagg ggtagccgga cgactcgagc ttggggtcga tgacggccag
     3061 cgccggtggc ggctcgtcgg cctgcagcac gtgatggtcg acaacgatca cgtcgacgcc
     3121 cttctcggcg gcataggcta tctcggcatg gttcgatatg ccgcagtcga cggtgacgat
     3181 gaggctgccg tagtcggcgg cgaactcatc gacggccgcc cgcgacagcc cgtacttctc
     3241 gtcctcggtc ggcaggcggt aacgcgcgtc gacgccgagc gaggcgagag cctccatcat
     3301 cagggtcgta gcggagacgc cgtcggcgtc gcggtcgccg aagacgagga ccttctcgcc
     3361 ttcgtcggcg gcgagcagga tacggtcgac cgcgtcctcc atctgggaga ataggaaggg
     3421 attgcgctgg aagcgcaggt cgtcctcgag gtagtacagc acctcctcgg gcgacaggac
     3481 gccgcgcctg gccaggacgg aggcagacag gaggtcgagg ccgaagcgct ccgccagctc
     3541 gcggatggct tgcggggaga cgtcgcgctt cttccacctc atagcgcggt gatttccttt
     3601 atcacgaggg gcgaggcggc cggggcttcg ggcccgacgg acagagcctc ggccaccagg
     3661 ggccaggccg cgtcgagcga ggcctcgtcc ttgccgtaga ccgtggcgac caggtcgccc
     3721 ttcgcgacgg cggcgccctt cctgcgctcg aagacgaagc cgacatccgg cgcgaccggg
     3781 tcgctcgtct tgttgcgccc gacgccgagg tagacgccgg ccaggccgat cttgtacgcg
     3841 tctatcgagc gcacataacc ggcggcgggg gcccgcagct cgcggacatt ccgggagcgg
     3901 aacgagccgc gcatcttcat catgcggtcg acgtcgccgc cctgggtcct tacgttgcgc
     3961 atgaacagct cgagcgcctt gcccgacgcc acggctttct cgcacagcct cgtcgcggct
     4021 tcgacgtcct gggccacgcc cgcggctccg ttgcctgcgg catcgttgcc tgcggcatcg
     4081 ttgcctgcgg caacgagcat ccaggcggcc aggcggtagg tcacctccat gaggtcggcc
     4141 ggccccctgc cctcgaggca gtcgatcgac tcctcgacct cgagcaagtt accgaccttg
     4201 tcgccgagcg gttcggtcat gtcggtgatc acgccgacga tgcgcttgcc catggcctga
     4261 ccg
//
LOCUS       DAOOLN010000023         4190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_25532_length_4190_cov_3.034825, whole genome shotgun sequence.
ACCESSION   DAOOLN010000023 DAOOLN010000000
VERSION     DAOOLN010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25532_length_4190_cov_3.034825"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PLW80_00580"
     CDS             complement(<1..591)
                     /locus_tag="PLW80_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB65024.1"
                     /translation="MATVCIHNATLLAGYAKMQSCAVLVEDGRIADVFSEKRLAQKRF
                     PAGAAVYDAHGAYIAPGFIDTHIHGFAGYGTEDMSVDAVMAMSDRLGAYGVTSFCPTI
                     YPMELGDMERSIEACVGAMGRETGAKVMGVHLEGPFVSMARLGVQRPEFVRPVDLEVM
                     ERLFKAGRGHITNMTVAPELKGMRELALYCLSRGIVL"
     gene            complement(644..2206)
                     /gene="nagZ"
                     /locus_tag="PLW80_00585"
     CDS             complement(644..2206)
                     /gene="nagZ"
                     /locus_tag="PLW80_00585"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HPB65025.1"
                     /translation="MLLIGYPGGAAGLRTLERVVSRRPMGNIILFSRNAKAPGPLRDE
                     LAYVRSMIAGQAGVEPLVSIDQEGGIVTRLGAGFTPLPGAMALAAAVSGGAATTADIE
                     AMATVNGSELKAVGIDWNLAPVADVNVNGLNPVIGVRSFGEDPEAVAELTAAYARGLS
                     KAGVASCAKHFPGHGDTSVDSHLDLPRVEADADRLERVELAPFRRLIAEGVSAIMSAH
                     VLFPAVEPEPIPATLSRKALTGLLRQRLGFRGLIVTDCLEMRAVGGRYDDLAARAILA
                     GADVLCVSHTAELQEEAFDSLLAAVRSGRIPESRVDESAARVLALKAVLASQASARDP
                     LGRPESLALATRLAEASISLVSGALPDLGGGCLYLDHRPALESRVEDGHEATPSMAAS
                     IGAAMADRAFGAIELPVDPDARAINAAVAAAAASSRPVVMGSYSLARHPAQAALAEAL
                     AAACDEAGVPLCFVATREPYDAAALSAIGGKERAVLCAYEYTELSTRAVARVLSGSAV
                     PAGTCPVSCRFL"
     gene            2402..3280
                     /locus_tag="PLW80_00590"
     CDS             2402..3280
                     /locus_tag="PLW80_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HPB65026.1"
                     /translation="MTSGVALLREIVDSCTESERKIAEYLIENPKTAVFCTIAELARQ
                     ADTNPPAVIRLCKRAGLSGYRELQILLTKDLYATTDQVDEEPPPAFELDSSQSVDAIA
                     RTIVSRTTDAVERVLSLLNTSAVEEAATAILGARRVAIFGVGASANVAYDLYQKLSRI
                     GVPCSFAFDADVQVSIACGLGGSDAAVAVSYSGRSAHTVTIVEEAKRSGARLIGITSV
                     GQSPLSRLADTALYVPATEPLLRTGASLSRATQLVMVDILYATIICRSIEVSIPRLER
                     SLKAVHEQDLKAVEPR"
     gene            3282..>4190
                     /locus_tag="PLW80_00595"
     CDS             3282..>4190
                     /locus_tag="PLW80_00595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB65027.1"
                     /translation="MTIRAVDPEAVAAELAALWNRSAGTAFPLDERLLRQQVSLDTDP
                     RLCLTVARPDGSLVAASLVKRAVRPGSDGAAPRIGYLSLLAVDEAERRRGLAAGLLAR
                     SVAWLASRGAERLELGGDYYHLVPGRPVEDGAGFEALDAFLRAVGFEGEGFEYDLSAD
                     LTRAAWLSEPERLPNGSYRYALYEPKYRGALLRFIGSSFPGRWSHEIREALDAGIRGA
                     DLMLAVDERDGSVVGFSRIYDKDSPLLGPGVYWRALMGEHPGGLGPIGVDPSRRGLGL
                     GMGLLEHCVRDLARRGVGTMVVDWTDL"
BASE COUNT          682 a         1395 c         1420 g          693 t
ORIGIN      
        1 gagcacgata cccctggaca ggcagtacag ggccagctcc cgcatgccct tgagctccgg
       61 cgccacggtc atattggtga tatggccccg tccggctttg aagagccgtt ccatcacctc
      121 gaggtcgacc gggcggacga attccggccg ttgcacgccg aggcgggcca tggagacgaa
      181 gggcccctcg aggtgcacgc ccatcacctt ggcgccagtc tcccgcccca tggccccgac
      241 gcaggcctcg atggagcgct ccatatcgcc cagctccatc ggatagatgg tcgggcagaa
      301 cgaggtgacg ccgtaggcgc cgagccggtc ggacatggcc atcacggcgt cgaccgacat
      361 atcctcggta ccgtagccgg cgaagccatg gatatgggtg tcgatgaagc ccggcgcgat
      421 ataggcgcca tgcgcgtcgt aaacggccgc gccggccggg aagcgtttct gggcgagccg
      481 cttctccgag aacacgtcag ctatcctgcc gtcctccacg agcacggcgc aggattgcat
      541 cttggcgtac ccggccagga gtgtagcgtt atggatgcat acggtagcca tcgtcggtct
      601 cctcggtatc cgtagctcta tcgaatatcg ccaatcccgt ccattaaagg aagcggcacg
      661 ataccggaca cgtgcccgcg gggacggccg agccgctcag aacgcgcgcg accgcccggg
      721 tcgatagctc ggtgtactcg taggcgcaga gcaccgcccg ctccttgccc ccgatcgccg
      781 acagggcggc ggcgtcgtac ggctcgcgcg tcgcgacgaa gcatagcggg acgcccgcct
      841 cgtcgcaggc ggcggcgagg gcctcggcca gggctgcctg ggccgggtgc ctggccagcg
      901 agtagctacc catgacgacc ggcctcgagc tggccgctgc cgcggctacc gcggcgttga
      961 tggctcgggc gtccgggtct accggcagct cgatcgcccc gaaggctcta tcggccatcg
     1021 ccgcgcctat cgaggccgcc atcgacgggg tggcctcgtg cccgtcctcg acgcggcttt
     1081 ccagagccgg cctatggtcg aggtagaggc agccgccgcc gaggtccggc agggctccgg
     1141 agacgaggct gatcgacgct tcggccagcc tggtagccag cgccaacgat tccggccgcc
     1201 cgagcgggtc tcgggccgac gcctgtgacg cgagaaccgc cttcagggcg agtaccctgg
     1261 ccgctgactc gtcgacgcgc gactcgggga tgcgcccgga gcgcacggcg gcgagcaacg
     1321 agtcgaaggc ctcctcctgc agctcggccg tatggctgac gcagagcacg tcggccccgg
     1381 ccaggatcgc gcgtgcggcc aggtcgtcat agcggccgcc gacggccctc atctcgaggc
     1441 agtcggtgac gatgagtccg cgaaagccaa gccgttggcg taggaggccg gtcagcgcct
     1501 tgcgcgacag ggtggccgga atgggctccg gttcgacggc ggggaacagg acatgggcag
     1561 acatgatggc cgagacgccc tcggctatca gccgcctgaa tggggccagt tccacgcgct
     1621 cgagccgatc ggcgtcggcc tcgacgcggg gtaggtccag gtgcgagtcg acgctcgtgt
     1681 ctccgtggcc cgggaaatgc ttggcgcagg aggcgacgcc ggccttggac agcccgcgag
     1741 cgtaggcggc cgtcagctcg gccaccgcct cgggatcctc gccgaagctg cgcacgccta
     1801 tgaccgggtt gaggccgttc acattgacgt ccgctaccgg cgccaggttc cagtcgatgc
     1861 cgacggcctt gagctccgag ccgttgaccg tggccatcgc ctcgatgtcg gccgtcgtcg
     1921 ccgcgccgcc ggagacggcg gcggccagcg ccatagcgcc gggcaggggc gtgaagccgg
     1981 cccccagcct ggtgacgatg ccgccttcct ggtctatcga gacgagcggc tcgacgccgg
     2041 cctggccggc tatcatcgag cggacgtagg ccagctcatc gcggagcggc ccgggagcct
     2101 tcgcgttgcg cgagaagagt atgatgttgc ccattggccg tcgcgacacg acgcgttcca
     2161 gcgtccggag tccggcggct cctcccgggt agcctatcag gagcatctgt ccgattttct
     2221 gttaagcgtc atattcgcga tggtcttcat gatgctagta tcttccattc ccaagaggat
     2281 cgcaacaata ttttatgaaa agccttgttt taaagaaatg atattccacg cttatagaaa
     2341 agcatggtat actacggctc attcgtgatg agtctaaccc gatggcgagg acaatgcaac
     2401 gatgacgagc ggagtggcgc ttctacgcga gatcgtcgat tcatgtaccg aatctgaacg
     2461 caagatcgcc gagtacttga tagaaaatcc taaaacggcg gtgttctgca ccatcgccga
     2521 actggccagg caggcggata ccaacccccc ggccgtgata cgcctctgca agcgggccgg
     2581 gctatccggt taccgggagc tacagatatt gctgaccaag gacctgtacg cgacgaccga
     2641 ccaggttgac gaagagccgc ccccggcgtt cgagctcgat tcaagccagt cggtggacgc
     2701 gatagccagg accatcgtct ccaggacgac cgacgcggtg gagcgggtgc tcagcctgct
     2761 caatacctcc gcggtcgagg aggcggccac cgcgatactc ggtgccaggc gcgtcgctat
     2821 cttcggcgtg ggcgcctcgg ccaacgtggc ctacgacctc taccagaagc tatcgcgcat
     2881 aggcgtcccc tgctccttcg cgttcgacgc cgacgtccag gtcagcatag cctgcggcct
     2941 cggcgggtcc gacgccgcag tcgccgtgtc gtactccggc agatcggccc acacggtcac
     3001 catcgtcgag gaggccaagc gctccggcgc taggttgata ggcattacca gcgtcggtca
     3061 gagccctctg tcgaggctcg ctgacaccgc gctctacgtg ccggccaccg agccgctgct
     3121 ccgtacgggc gcctcgctgt cgagggccac ccagctggtc atggtggaca tcctgtacgc
     3181 caccatcata tgccgtagca tcgaggtgtc gatcccgagg ctcgagcgta gcctgaaggc
     3241 cgtgcatgag caggacctga aagccgtgga gccacgttag catgacgata cgggccgtag
     3301 acccagaagc cgtcgccgcc gagctggcgg ccctgtggaa ccgctcggcc gggaccgcct
     3361 tcccgctcga tgagcggcta ctgcgccagc aagtatcgct cgataccgat ccccggctat
     3421 gcctgactgt cgccaggccc gacggatccc tggtagcggc ctcgctggtc aagcgggccg
     3481 tccggccggg gtcggatggc gccgcgccca ggatcggcta cctgtctctc ctggcggtgg
     3541 acgaggccga acggaggcgg ggtctcgccg ccgggctcct ggcccggtcg gtcgcctggc
     3601 ttgcgtcgcg gggtgccgag cgactcgagc tcggcggcga ctattatcac ctcgtacctg
     3661 ggcggcccgt cgaggatggg gccggcttcg aggctctcga cgcctttctc cgggccgtcg
     3721 gcttcgaggg cgagggcttc gaatacgacc tgtcggccga tctgacccgc gccgcctggc
     3781 tgagcgagcc cgagaggttg ccgaacggtt cgtaccgcta tgcgctctac gagcccaagt
     3841 accgtggcgc cctcctgcgc ttcatcggct cgagcttccc ggggcgctgg tcgcacgaga
     3901 tccgggaagc cctcgacgcg ggtatccgcg gcgccgacct gatgttggcc gtggatgagc
     3961 gcgacggatc ggtggtcggc ttctcgcgta tatacgataa ggacagcccc ctgctcgggc
     4021 cgggggtgta ttggcgggcc ctgatggggg agcaccccgg cgggctcggc cccatcggcg
     4081 tcgatccatc caggcgaggg ctcgggcttg ggatgggcct gctggagcac tgcgtccgcg
     4141 acctggcgcg aaggggcgtc ggcacgatgg tcgtcgattg gaccgacttg
//
LOCUS       DAOOLN010000024         4127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_26046_length_4127_cov_3.160855, whole genome shotgun sequence.
ACCESSION   DAOOLN010000024 DAOOLN010000000
VERSION     DAOOLN010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26046_length_4127_cov_3.160855"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PLW80_00600"
     CDS             <1..496
                     /locus_tag="PLW80_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65028.1"
                     /translation="PSAIATIGSPAALAAWLRHGLVVNPLVYLARTIGAFVPSIGAGL
                     PDPERVGEDGDGAWKGHRGLYPRQTYTLQIGLRAMERRLGDVTCPVLVCHARGDRMVD
                     FRNAAVIESGLGSDDIEAYVANMDGFGHMRHNLLLYDSQRDRVWARILDFFERRAPLT
                     RGRA"
     gene            498..2468
                     /locus_tag="PLW80_00605"
     CDS             498..2468
                     /locus_tag="PLW80_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPB65029.1"
                     /translation="MVRGEDGWFIDEGGRRLILRGCNLGGDCKTPYRARTALPSPGEL
                     YDYRAASFVGRPLPEAEAGEHLDRLARWGFNAVRLLVTWEGLEPTGPGEYDDTYYDYI
                     ERLVAMAAERGLRLFVDPHQDAWSRWTGGDGAPAWTLERVGFDVTRLHRSGAAFVHEE
                     AGDPYPRMRWPSNYSRLACQTMFTLFFAGDDYAPSLRIDGLDSRSFLQGSYLAAVARL
                     AGRLARYDAVIGVDTLNEPSDGYIGLTDLSRLQRAMAKTGPMPSPFEAMATGSGYPTE
                     VDVYGVKGLGQGVVGRETLGEHGVSAWKDGTECLWRREGVWDVACGKPVLKRPDYFAR
                     ARGAEPDFAEHYLRPFARRVGEAVSKAAGAGRSMLFIESVPTVAAPRWDAEDAKASGV
                     SGAANAGHWYDGFTLFMKRRVPFIAYDQEEERVILGRGAVRRYYAEHLGRLKRRGLVD
                     MDGAPTLIGEFGLPYDLNGAAAYRTGDYRVHVEALSAYYDAMDENLLSATIWNYTASN
                     THERGDGWNGEDLSIWCRDDHEAGRTESGDPADAGGRALAGFVRPYARATAGLPRSMR
                     FDRKSGRFEYVFEPRPTGVQARGAGPKAGTEAQALITELYVPEVQYPRGFSIRTTGCA
                     HAVEDRPGHALVLVRAEPGATRCRVVVERARA"
     gene            complement(2513..>4127)
                     /locus_tag="PLW80_00610"
     CDS             complement(2513..>4127)
                     /locus_tag="PLW80_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65030.1"
                     /translation="FWFDGTAAPDFIRPDQAGGTGIPLGKVKVVRVFDEKRWMLVRGF
                     EGLAEGDSIRVHAHGDLNRTTARVRGVMDKPEGLFLQVDCEFGPEDEVYLVQTKSMVK
                     RYKPVLPASLAKYHRFPSFDKAPRPEFPAIPKERLAVLPDGLYTMVSRVQDLHTILTD
                     RPEKAVLRLSRKNAELLRRHEKETPFKRDSLVLWLEPFFPQADTAWLAEELDYWIGKG
                     QRLFVANNLGHLAMLKGREVTVIAGPWLYAFNAWAAAYLLGNGADFIMPPLEISKQAF
                     GLLAETVPGKSLFPVVFAYPQLFTIRADLGERFDLRFFRDREGDEYELVSSPDGSVVI
                     PLKPFSLVDRIPFIRKDGATKFLLDFSFIELRKQVYKRVVAAARDGLVLPDSGRFNWK
                     DGFWNPEEERASRSADSGDRGPRSAPKAGSGGRPTTAGKADGKPDAKPGAAAAPAKRQ
                     RLDRPGRGGPLWKVNLARERQEERKSDRRDEARRESRQSDRTSANAPARKPSGAGNGG
                     PAGKRPGGSGSGQRPAGRDSRGPSRKPRG"
BASE COUNT          595 a         1442 c         1493 g          597 t
ORIGIN      
        1 cccgtcggcg atagcgacga taggctcgcc ggcggcgctc gcggcctggc tccggcacgg
       61 cctcgtcgtc aacccgctcg tctacctggc ccggaccatc ggcgcgttcg tgccgtcgat
      121 cggcgccggc ctgcccgatc ccgagcgcgt cggcgaggac ggcgacggcg cctggaaagg
      181 ccaccgggga ctctacccga ggcagaccta cacgctccag atcggcctgc gagccatgga
      241 acggcggctc ggcgacgtga cctgccccgt gttggtctgc cacgcccgag gcgaccgcat
      301 ggtcgacttc aggaacgcgg ccgtcatcga atcggggctc ggctcggacg atatcgaggc
      361 ctacgtggcc aacatggacg ggttcggcca catgcggcac aacctgctgc tctacgacag
      421 ccagcgcgac cgggtctggg cgcgcatcct ggatttcttc gagcggcggg cgccgctgac
      481 gagggggagg gcctagcatg gtacggggcg aggacggttg gttcatcgac gagggcggca
      541 ggaggctgat cctgcgcggc tgcaacctgg gcggcgactg caagacgccg taccgcgcgc
      601 gtaccgcgct accgagcccg ggcgagctgt acgactaccg cgccgcgagc ttcgtggggc
      661 ggccgctgcc cgaggccgag gccggggagc acctggaccg cctggcgcgc tggggcttca
      721 acgcggtcag gctcctcgtc acctgggagg gcctcgagcc gaccgggcca ggcgaatacg
      781 acgatacgta ttacgattat atagagcgcc tcgtcgcgat ggccgccgag cgcggcctcc
      841 gcctgttcgt cgacccgcac caggacgcct ggagccgctg gaccggcggc gacggcgcgc
      901 cggcctggac cctcgagcgg gtcggcttcg atgtaacgcg cctccaccgc tccggagcgg
      961 ccttcgtcca cgaggaggcg ggagacccct acccgcgcat gcgctggccg tcgaactact
     1021 cccgcctggc ctgccagacc atgttcacgc tgttcttcgc cggcgacgac tacgcgccga
     1081 gcctccggat cgacgggctc gattccagga gtttcctgca gggcagctac ctagccgccg
     1141 tggccaggct ggccggccgg ctggcccgct acgacgcggt cataggcgtc gataccctca
     1201 acgagccgtc cgacggctac ataggcctga ccgacctgtc gaggctccag cgggcgatgg
     1261 ccaagaccgg cccgatgccc tctcccttcg aggcgatggc gacgggatcc ggctacccga
     1321 ccgaggtgga cgtctacggg gtcaagggcc tcgggcaggg ggtcgtcggc cgcgagacgc
     1381 tcggcgagca cggcgtctcc gcctggaagg acggaacgga atgcctctgg cggcgcgagg
     1441 gcgtctggga cgtcgcctgc ggcaagcccg tgctcaagcg cccggactac ttcgcccggg
     1501 cgcgcggcgc cgagccggat ttcgccgagc actatctgcg gcccttcgcg aggcgggtcg
     1561 gcgaggcggt gtcgaaggcc gccggggcgg gccgctccat gctgttcatc gagagcgtgc
     1621 cgaccgtggc ggcccctcga tgggacgccg aagacgcgaa ggcctcgggc gtgtcgggcg
     1681 cggcgaacgc cggccactgg tacgacggct tcaccctgtt catgaagcgg cgcgtgccct
     1741 tcatcgccta cgaccaggag gaggagcgcg tcatcctggg ccggggggcg gtcaggcgct
     1801 actatgccga gcatctgggc cggctcaagc gccgcggcct cgtcgacatg gacggcgcgc
     1861 cgacgctgat aggcgagttc gggctgccct atgacctgaa cggggccgcg gcctaccgca
     1921 cgggcgacta ccgcgtgcac gtggaggccc tgtcggcgta ctacgacgcg atggacgaga
     1981 acctgctgtc agcgacgatc tggaactaca cggcgagcaa cacgcacgag cgcggcgacg
     2041 gctggaacgg cgaggacctg tcgatctggt gccgcgacga ccacgaggcc ggccggaccg
     2101 agtcaggcga cccggccgac gccggtggaa gggcgctggc cggcttcgtg cggccctacg
     2161 cccgggccac ggccggcctg ccccggtcga tgcgcttcga ccggaagagc ggccgcttcg
     2221 agtacgtctt cgagccccga ccgaccggcg tccaggcgcg cggggccggc cccaaggccg
     2281 gaaccgaggc ccaggccctg atcaccgagc tgtacgtgcc cgaggtccag tacccgcgcg
     2341 gcttctcgat acggacgacg ggctgcgccc acgccgtcga ggaccggccg ggacacgcgc
     2401 tcgtgctcgt gcgggccgag cccggcgcta cccgttgccg ggtcgtcgtc gaacgcgccc
     2461 gggcctagcc gccgcctcag ccgcgcaagc cgcctcagca gcgcaagccg cctcagccgc
     2521 gcggcttgcg cgaggggccg cgggagtcgc ggccggccgg gcgctgacct gagcctgaac
     2581 cgccgggccg cttgccagcc gggccgccgt tcccggctcc ggacggtttc ctggcgggcg
     2641 cgttggcgct cgtccggtcg gactgcctcg attcgcgccg agcttcgtcg cgccggtcgg
     2701 attttcgttc ttcctgccgc tccctggcca ggttcacctt ccagaggggg ccgccgcggc
     2761 cgggacggtc gagccgttgg cgcttcgccg gagccgccgc ggcgccaggc tttgcgtcgg
     2821 gcttgccgtc ggccttgccg gccgtcgtcg gcctgccgcc ggagccggcc ttgggcgcgg
     2881 agcggggacc gcgatcgccc gagtcggccg agcgcgaggc ccgctcctcc tcggggttcc
     2941 agaagccgtc cttccagttg aagcgccccg aatcgggtag cacgagcccg tcgcgggccg
     3001 ccgcgacgac gcgcttgtac acctgcttgc gcaattctat gaagctgaag tccaggagga
     3061 acttcgtcgc tccgtccttg cggatgaagg ggatgcggtc gacgagggag aagggcttga
     3121 gcggtatgac cacggagccg tcgggcgagg agacgagctc gtactcgtcg ccctcccggt
     3181 cgcggaagaa gcgcaggtcg aagcgctcgc ccaggtcggc gcgtatcgtg aacagctgcg
     3241 ggtacgcgaa gaccaccggg aacaggctct tgcccggcac cgtctcggcg agtagcccga
     3301 aggcctgctt ggagatctcg agcggcggca tgataaagtc ggccccgttc ccgagcaaat
     3361 aagccgcggc ccaggcgttg aaggcgtaca gccaggggcc ggctatcacc gtgacctcgc
     3421 ggcccttgag catggccagg tggccgagat tgttggcgac gaagagccgc tgccccttgc
     3481 ctatccagta gtcgagctcc tcggccagcc aggcggtgtc ggcctggggg aagaacggct
     3541 ccagccagag gacgagcgag tcgcgcttga acggcgtctc cttctcgtgc cgcctgagca
     3601 gctcggcgtt cttgcgcgac agcctgagca cggccttctc cggccggtcg gtcagtatcg
     3661 tgtgcaggtc ctgcacccgg ctgaccatcg tgtagaggcc gtcgggcagg accgccaggc
     3721 gctccttcgg gatggccggg aactcgggcc gcggggcctt atcgaagctc gggaagcggt
     3781 ggtacttggc cagcgacgcg ggcaggacgg gcttgtagcg cttgaccatc gacttggtct
     3841 gcaccaggta gacctcgtcc tcggggccga actcgcagtc tacctgcagg aacagcccct
     3901 cgggcttgtc catcacgccg cggacgcggg ccgtcgtgcg gttcaggtcg ccgtgggcgt
     3961 ggacgcggat cgagtcgccc tcggctaggc cctcgaagcc gcgcacgagc atccagcgct
     4021 tctcgtcgaa gacgcggacc accttgacct tgccgagcgg gatgccggta ccgccagcct
     4081 ggtccggccg gatgaagtcg ggcgccgccg tgccgtcgaa ccaaaac
//
LOCUS       DAOOLN010000025         4084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_26424_length_4084_cov_2.443286, whole genome shotgun sequence.
ACCESSION   DAOOLN010000025 DAOOLN010000000
VERSION     DAOOLN010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26424_length_4084_cov_2.443286"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2495)
                     /locus_tag="PLW80_00615"
     CDS             complement(<1..2495)
                     /locus_tag="PLW80_00615"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPB65031.1"
                     /translation="MSRTVLKPFQGAAVDNAAAVLGGCLRMLDALRGSADYEGRRGAV
                     IRDSGSILIEAPTGIGKTLMAAHAVSRLAADRPMLWLWFAPFSSVVSQTESVLADETE
                     NLRPRDPHTDRAVEELRPGDVFISTWASVAVTDAEIRKTRSTTENMPSLDLLVAHSKA
                     AGWYVGVVIDEAHHSFRGQSKAYDFFREVLDPDLSLLVTATPRDEDIESFKSGVHLGN
                     LRRIRVRRQEGVDAGLLKVGVKTAVFKAPDSVAELLDFKLMALQQGVAVHRRLKARLA
                     DSGVAMTPLLMVQVDSADDAVREAEAWLKDLGFAKNQVMVHTAKEPDPFLNSVQGDDE
                     VEVLVFKMAVALGFDAPRAFTLVSFRSSRDPDFGLQIVGRLMRVDRRLQRILPVAPEL
                     MHGYVFLSDKSSQEGIVTAGDRINAIRNELASLETSVDVIALGTDDAALVSVRNGQPE
                     LPGVSYGSDHAGFPAGEPRQPAYAERSLFAEWGLTPGGTGSGSIAKVPAAVSRPVQAS
                     SGFSWALKQSAPRAFTRAEVDVLSFDALTDIYNRFRWDADTLLLAKIDSSKVLMERYE
                     VFSHLREAPEEIRARLFKDELSRRSQLCLRNADRDGFLDQRALYAGLMDRLAAEASDK
                     GISGLESREELEDGLAHILALRPDLLTKAVSESFAAHTVSVQADDLPSAIVSSEPLIG
                     SRLNIYGVYPSDLNNWERPFAERLDNDLSGAVLWWHRNPVHKRHSVSLPVAGHGFYFP
                     DFIVGVKGRSTPGGVILVEIKYQINDPIGNAAAKSRACHPDYGSVLMLYLDDKSGQWM
                     TVVYDERKGQNVLDRVFREDQLVLIAHPQHPLA"
     gene            complement(2492..>4084)
                     /locus_tag="PLW80_00620"
     CDS             complement(2492..>4084)
                     /locus_tag="PLW80_00620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPB65032.1"
                     /translation="FLGAAYKGRVKCVYIDPPYNTGTTDFIYNDRYQNKEDAFFNSTW
                     LEFLYRRLSLARDLLREDGVLLVSINDANRAYLELLLEQIWPGGRQGTLVWRNRQGSN
                     ADQGCFLSSDHEHVLVWSNPGFKFKGFEKSYEMYSNPDNDPKGDWRSDNLTLGFTYVD
                     RPNLYYPLCDPKTDIWYPANPDGVWRYASKERLKEGQQVQAKPMEDFIALGQIRFPIE
                     QCVVVWETKEALLKAIELGEVPCSGKTPLLRKELPDLDFWVGKKVGFDRPQFKRYKAD
                     LRNENQPLSSWVSSTIDKEDELGNSLVSGTNQEGTKALLKQLGDKAFSYPKPPSLLRG
                     LLSQSTGPDDLVLDFFAGSGTTAQAVAELNAEDGGHRACILVSSTEATADEPGKNICR
                     DVAARRLAALGREFAYLRMESYPVGDHGRALSDAAVWTALQLFVAQEAGAAPDDNEAL
                     WTRETEQCLLCFARRLDAGSLAAIAALAPPPGKVLLVFSFQPGPLRQRLIGRSDARVE
                     ALPDALERALLPASAKRAGGAL"
BASE COUNT          741 a         1278 c         1304 g          761 t
ORIGIN      
        1 ttcgccagcg gatgctgcgg gtgcgcgatg agcacgagct ggtcctcgcg gaacacgcgg
       61 tcgaggacgt tctggccttt acgctcgtcg tagacgacgg tcatccactg gccgctctta
      121 tcgtcgaggt agagcatcaa gacagaaccg tagtccgggt gacaggcgcg gctcttggcc
      181 gcggcgttgc ctatcgggtc gtttatctgg tacttgatct ccacgaggat gacgccgccc
      241 ggcgtggagc ggcccttgac gccgacgatg aaatcgggga agtagaaacc gtggccggcc
      301 accggcaaag acactgaatg cctcttgtgc acaggattcc ggtgccacca gagcacggcg
      361 cccgacaggt cgttgtccag gcgctcggcg aatggccgct cccagttatt caggtccgaa
      421 gggtacacgc catagatatt gagccgggaa ccgatgagcg gctcggagga tacgatagcg
      481 gacggaaggt cgtcagcctg gactgaaacc gtatgcgcgg cgaaggattc gctgacagcc
      541 ttggtcagga ggtccggcct gagcgcgagt atatgcgcca acccgtcttc aagttcttcg
      601 cggctctcca acccggatat acccttgtcg gacgcctcgg ccgcgagccg atccatcaag
      661 ccggcgtaga gggctcgctg atccaggaag ccgtcccggt ccgcgtttct caggcatagt
      721 tgcgaacgtc gcgacagctc gtccttgaac agcctcgccc gtatctcctc gggagcctcc
      781 cgcaggtggc taaagacctc gtaacgctcc atcagcacct tgctgctatc gatcttggcg
      841 agcaacagcg tatcggcgtc ccacctgaac cggttataga tgtccgtcag tgcgtcgaaa
      901 gacagcacat ccacctcggc gcgggtaaag gcgcgcggcg cggactgctt gagggcccag
      961 ctgaaaccgg atgaagcttg gacgggccga gatacggccg cgggtacctt agcgatggaa
     1021 ccggagccgg tcccgcccgg agtcaagccc cattcggcga acagcgatcg ttcggcgtag
     1081 gccggctgac gaggctcgcc agccgggaag ccggcgtgat cgctcccata gcttacgccg
     1141 ggtagctcag gctggccatt ccgcacgctc accagcgcgg cgtcgtccgt tccgagggct
     1201 atgacgtcca ccgacgtctc gagcgaggcc agttcgttcc tgatggcgtt gatgcggtct
     1261 cccgccgtga cgatgccctc ctgggacgac ttgtcggaga ggaagacgta gccgtgcatc
     1321 agctctggcg ccacgggcag gatacgttgg agccggcggt cgacgcgcat gaggcggccg
     1381 acgatctgga ggccgaagtc cgggtcgcgg ctggaacgga agctgaccag cgtaaaggcg
     1441 cgcggcgcgt cgaagccgag ggcgaccgcc attttgaaga ccaggacctc gacctcgtcg
     1501 tcgccctgca ccgagttgag gaaggggtcg ggctccttgg ccgtatgcac catgacctgg
     1561 ttcttcgcaa agcccaggtc cttgagccag gcctcggcct cgcgcacggc gtcgtcggcg
     1621 gagtcgacct gcaccatgag gaggggcgtc atggctacgc ccgagtcggc gagcctggcc
     1681 ttgagccgcc tatgcaccgc gacgccctgc tgcagggcca tcagcttgaa gtccagcagc
     1741 tccgcgacgc tgtcgggcgc cttgaagacc gcggtcttga cccccacttt gaggagtccc
     1801 gcgtcgacgc cttcctgacg cctgacgcgt atgcgccgca ggttcccgag gtggacgccg
     1861 ctcttgaagc tctctatgtc ctcgtccctc ggggtcgcgg tgacgagcag gctcaggtcc
     1921 gggtcgagca cctcgcggaa gaagtcgtag gccttggact ggccgcggaa ggagtggtgg
     1981 gcctcgtcta tcacgacgcc gacgtaccag ccggcggcct tggagtgcgc gacgagcagg
     2041 tccagggacg gcatgttctc ggtggtggag cgcgtcttgc gtatctcggc gtcggtgacg
     2101 gcgacggagg cccaggtgga tatgaagacg tcgccgggcc gcagttcctc cacggcccgg
     2161 tccgtgtgcg ggtcgcgggg ccggaggttc tccgtctcgt cggcgaggac cgactcggtc
     2221 tgcgatacga ccgacgagaa cggcgcgaac cacagccaca gcatgggcct gtcggcggcc
     2281 agcctggaga cggcgtgcgc ggccatcagc gtcttaccta tgcccgtggg cgcctctatc
     2341 agtatggaac cggaatcgcg tatgaccgcg ccccgccgac cttcgtagtc ggccgagccg
     2401 cgcagggcgt cgagcatgcg caggcagccg cccagcacgg ccgcggcgtt gtcgacggcg
     2461 gcgccctgga agggcttgag gacggtgcgg ctcatagcgc gcccccggcg cgcttggcgc
     2521 tcgccggtag cagggcccgt tcgagcgcgt cgggcagagc ctccaccctg gcgtccgacc
     2581 gtccgatcag ccgctggcgc agcggccccg gctggaacga gaacacgagc aggaccttgc
     2641 ccggaggcgg cgccaacgcc gctatagccg cgaggctccc ggcgtccaat cgtcgcgcga
     2701 agcacaggag gcactgctcg gtctcccgcg tccatagagc ttcattatcg tcgggcgcgg
     2761 cgcccgcttc ctgggccacg aagagctgga gggccgtcca gacggcggcg tccgacagcg
     2821 cccgcccgtg gtctcccacc ggatagcttt ccatacggag gtaggcgaac tcccggccaa
     2881 gcgccgcaag gcggcgcgcc gccacgtcgc ggcagatgtt cttgccaggc tcgtctgccg
     2941 tggcctcggt actggacacg aggatgcagg ccctgtgccc gccgtcctcg gcgttcagct
     3001 ccgcgaccgc ctgcgccgtc gtgccggagc ccgcgaagaa gtccagcacc aggtcgtccg
     3061 gcccggtgga ttgcgacaga agaccacgaa gaagagaagg cggctttgga tacgagaacg
     3121 ccttgtcacc caactgtttc agtaatgcct ttgtaccttc ttgattggtt cccgatacga
     3181 gactgtttcc cagctcgtct tccttgtcga tagtggaact cacccagctc gagagtggct
     3241 gattctcgtt gcgcaaatcg gccttgtagc gcttaaactg tggacgatcg aatccaacct
     3301 ttttacctac ccagaaatct aaatccggca attctttcct cagaagaggg gttttacctg
     3361 aacaaggtac ttcaccaagt tctatcgcct ttaatagagc ttccttcgtc tcccatacaa
     3421 ctacacactg ttcaatagga aaccgtatct gacctagcgc aataaaatct tccatcggtt
     3481 tcgcttgaac ctgctggcct tccttgaggc gttctttact cgcgtatcgc cagacaccat
     3541 cgggattagc aggataccaa atatcggtct tgggatcaca aagaggatag taaaggtttg
     3601 ggcgatctac gtatgtaaaa ccaagtgtga gattatctga acgccaatct cccttaggat
     3661 cattatctgg gttcgaatac atctcatagc ttttctcaaa gcccttgaac ttaaatcctg
     3721 gattagacca gacgaggaca tgctcatggt cgctactcag gaagcaaccc tggtcagcgt
     3781 tcgatccctg cctgttgcgc cacacgagcg tgccctgtcg cccgcccggc cagatctgct
     3841 ccaagagcag ttccaggtag gcgcggttgg cgtcgttgat gctgacgagg aggacgccgt
     3901 cctcgcgtag gaggtcgcgg gccagcgaca ggcggcggta caggaactcc agccacgtgg
     3961 aattgaagaa ggcgtcttcc ttgttctggt agcggtcgtt gtagatgaag tccgttgtgc
     4021 cggtgttgta cggggggtct atatagacgc acttgacccg gcccttgtag gccgcgccca
     4081 ggaa
//
LOCUS       DAOOLN010000026         4076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_26489_length_4076_cov_3.236260, whole genome shotgun sequence.
ACCESSION   DAOOLN010000026 DAOOLN010000000
VERSION     DAOOLN010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26489_length_4076_cov_3.236260"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PLW80_00625"
     CDS             <1..388
                     /locus_tag="PLW80_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPB65033.1"
                     /translation="FLDRNLTFELFDAIDAAKIALSVGQESEIAFALPPHVSFVERLT
                     RAEFEGVIGRRVDEARALVLEALERSGRRPADVSTVIRVGGSSRVPAFAAMLESLFPG
                     RVREGAVFTSIASGLLAAHDSGLSAA"
     gene            complement(385..2883)
                     /locus_tag="PLW80_00630"
     CDS             complement(385..2883)
                     /locus_tag="PLW80_00630"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB65034.1"
                     /translation="MPDYSDFLKRQARDSLSTDALYAIHGETAFGNPAWASARSRILI
                     VRLSPFRDVERSTPHEFLFREARAAMPEAYMDFCFFPTRRDRAVLDAAGVPWMHGLAS
                     GRGAIEYDALLVSNSYTLELVNLAPFLEASGIPASRRERDAAVSAAADGARFPLIALG
                     GSNAMASAAQYDEASGDAIVDAVFFGEGEGAVRELIPGLAAAALAGGSSRLAALETLA
                     RRVRGFWPTALRIEVDQAKAPADAYPQGAPPVLAGAEAGTSRLEITRGCPSFCSFCFE
                     GWERKPFRERPLASVVAEAKRLKAATGVDTVELASYNFNAHGEVVAVIESLHKIFWGV
                     GFQSQRVDILARSPSLVRFEVAAGKRSFTVGVEGVSSRMRAYYSKGLDEADLRSVLER
                     LVREGARELKLFYILSGYEADDDLAELSAFVGWLRGVVAAQAAPPRVMFSAGELVRMP
                     FTPLRYEALILDEAVYAAIRSRFEAIVESGGFEARAPECFDEYCLSQLLASAPDGSFA
                     LLLAMAERGYVYDRTLSAGAWEFARERLLGSAERLATLVAEKPRHYRFPYSLVRPLVS
                     GDRIYERFLDARSNLERPSCLGGDCAACGACQGDERAFLAAHRLGSATEADLRAVEAV
                     VAAKRRPYETWVRSTLPREAATADPAYPATLFRRDLYAALPALVDTVWTAEDALLKNK
                     TGLERLPGAWGDTWYRVLSSAPIDPDALETAGYAVGSAAPAPERLYLRVSLAGASHAE
                     AARLVAGFLDASAIPHTLRKTAAATVFAVSDKGRKKRNILEASIPASLQASVQGRAII
                     DLACGSKYDVANLMKAAAKRGLYPELRVGLEPLP"
     gene            complement(2894..3388)
                     /locus_tag="PLW80_00635"
     CDS             complement(2894..3388)
                     /locus_tag="PLW80_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPB65035.1"
                     /translation="MVDTAALQAYSLFGGLSAEQIGLIRPLMGTAIFEAGEVIIREGQ
                     PNDSIYFITEGRVDVTRCGVSIVELPEGQTFGEMELLDVMPTAATVTAVMPVKVVTIS
                     NRCVREVYARDPKVFGIVMMNLARDLSRRLRRMDELACDASLPNHTAFLRVPAGDEPA
                     EYAR"
     gene            3470..>4076
                     /locus_tag="PLW80_00640"
     CDS             3470..>4076
                     /locus_tag="PLW80_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65036.1"
                     /translation="MHAIRQRSEAAKVRRTFYSISLLIVIVVFIVFTTSHFTSLKRQN
                     DESFKRLEELMVTMTKVYLKDTVSRTLADIDLERKMIRLDLQVAAGDAEAESRADELV
                     KARSIVRIRETRLIEDGYLWASELLTPAGGRDYARRIAHGRYPDSEGTLLSTEASSGL
                     NASQYREELDGLLADGEVYYTYYTPKNGEPLPSRMLVYAALY"
BASE COUNT          717 a         1344 c         1390 g          625 t
ORIGIN      
        1 gttcctcgac cgcaacttga ccttcgagct gttcgacgcc atcgacgcgg ccaagatcgc
       61 gctatcggtc ggccaggaat cggagatagc cttcgccctg ccgccgcacg tctcattcgt
      121 cgagcgtctg acgcgggccg agttcgaggg cgtcataggc cgcagggtcg acgaggcgcg
      181 cgccctggtg ctcgaggcgc tcgagcgctc ggggaggcgg ccagccgacg tgtcgacggt
      241 catccgcgtc ggcggctcgt cacgcgtgcc ggccttcgcc gccatgctcg agtcgctgtt
      301 cccgggacgc gtccgggaag gcgccgtgtt cacgagcatc gcttccgggc ttctggccgc
      361 tcacgattcg ggcctatcgg ctgcctaggg tagcggttca agccctaccc gcaactcggg
      421 gtagagccct cgcttggccg cagccttcat caagttggct acgtcgtact tcgagccgca
      481 ggccaggtcg atgatggccc ggccctgaac ggaagcctgg agagaagccg gaatggaagc
      541 ctcgagtatg ttgcgcttct tcctaccctt gtcggacacc gcgaagaccg tagcggccgc
      601 ggtcttcctg agcgtatgcg ggatagccga cgcgtccagg aagcccgcga caagcctcgc
      661 ggcctcggcg tggctggcgc cggccaggct cacacgcagg tacaggcgct cgggggccgg
      721 cgccgcggat ccgacggcgt agcctgccgt ctcgagcgcg tccggatcga tcggggccga
      781 cgacaggacg cggtaccagg tatcgcccca ggcgccaggc aggcgctcga gccccgtctt
      841 gttcttgagc aaggcgtcct cggcggtcca taccgtatcg acgagggccg gcagcgcggc
      901 gtacaggtcc ctacggaaca aggtcgccgg atacgccgga tcggccgtcg ccgcctccct
      961 gggcagggtc gagcggaccc aggtctcata cggccggcgc ttggccgcga cgacggcctc
     1021 gacggcccgg aggtcggcct cggtagccga gccgagccta tgagcggcca ggaaggccct
     1081 ctcgtcgccc tggcaggcgc cgcaggccgc gcagtcgccg ccaaggcagc tcggccgctc
     1141 caggttactc ctggcgtcca ggaagcgctc gtagattcgg tcgccggaga cgaggggacg
     1201 cacgagcgag tacgggaacc ggtagtggcg gggcttctcg gcgaccaggg tcgccaggcg
     1261 ctcagccgag ccgaggaggc gttccctggc gaattcccag gcgccggccg atagggtacg
     1321 gtcatagacg tagccgcgct cggccatcgc caggagtagg gcgaaggagc cgtccggcgc
     1381 cgacgcgagg agctgggaca ggcagtactc gtcgaaacac tccggggcgc gcgcctcgaa
     1441 gccgcccgat tcgacgatgg cctcgaagcg ggagcggatg gcggcgtaga cggcctcatc
     1501 gaggatcagc gcttcgtacc ggagcggcgt gaacggcatg cgcacgagct cgccagcgga
     1561 gaacatgacc cgcggcgggg cggcctgggc cgcgacgaca ccacgtagcc acccgacgaa
     1621 ggccgatagc tcggccaggt cgtcgtcggc ctcgtagcct gataatatat agaagagctt
     1681 gagctcgcgg gcgccctcgc ggaccaggcg ctcgagcacc gatcgcaggt cggcctcgtc
     1741 gagccccttg gagtagtagg cgcgcatgcg cgacgatacg ccctcgacgc cgaccgtgaa
     1801 cgatcgctta ccggccgcga cctcgaaacg taccagggac ggcgagcgcg ccaggatgtc
     1861 gacgcgctgg ctctggaagc cgacgcccca gaaaatcttg tgaagggact cgatgacggc
     1921 gacgacctcg ccgtgggcgt tgaagttata gctggccagc tcgaccgtat cgacgccggt
     1981 ggccgccttg aggcgcttgg cctcggcgac gactgaggcg agcggccgct ccctgaacgg
     2041 cttgcgctcc caaccctcga agcagaacga gcagaaactc gggcagccgc gggtgatctc
     2101 gagccggctc gtaccggcct ccgcgccggc taggaccggt ggcgctccct gggggtaggc
     2161 gtccgccggg gccttggcct gatccacctc gatgcgcagg gccgtcggcc agaagcccct
     2221 aacccgccgg gcgagcgtct caagcgccgc gagcctggac gagccgcccg ccagagccgc
     2281 cgccgctaga cctggtatca gctcgcggac ggcgccctcg ccctcgccga agaacacggc
     2341 gtcgacgatc gcgtcgccgg aggcctcatc gtactgggcg gcgctggcca tcgcgttcga
     2401 gccgccgagc gcgatcagcg ggaaacgagc gccgtcggcc gccgccgaga cggcggcatc
     2461 ccgctctcgc ctcgacgccg ggatgcccga cgcctccagg aagggcgcga ggttgacgag
     2521 ttccagggtg taggagttcg agacgagcag cgcgtcgtac tcgatcgccc cgcggcccga
     2581 ggccaggccg tgcatccacg gcacgccggc cgcgtcgagg acggcccggt cgcgcctggt
     2641 cgggaagaag cagaagtcca tataggcttc gggcatggcg gccctcgcct cccggaacag
     2701 gaattcatgg ggcgttgagc gctcgacgtc gcggaatggc gacagccgga cgataaggat
     2761 gcgcgagcgg gccgaggccc aagccgggtt cccgaaggcc gtctcgccat ggatggcgta
     2821 cagagcgtcg gtcgagaggc tgtcgcgcgc ctggcgcttg aggaaatcgg agtaatcggg
     2881 caccgcgtct accctatcgg gcgtattcgg ccggctcgtc gccagccgga acccgcagga
     2941 aagcggtgtg gttcggcaac gaggcgtcgc aggccagctc gtccatccgc cggagacgcc
     3001 tcgacaggtc gcgggccagg ttcatcatga cgatgccgaa gaccttcggg tcgcgggcgt
     3061 agacctctcg cacgcaacgg ttggatatgg tgacgacctt gaccggcatg acggccgtga
     3121 cggtggcggc cgtcggcatt acgtcgagca gctccatctc gccgaaggtc tggccctcgg
     3181 gcagctcgac gatggacacg ccgcaacgcg tgacgtccac gcggccctcg gtgatgaagt
     3241 agatcgaatc gttgggttgg ccttcgcgga tgatgacctc gccggcttcg aagatcgcgg
     3301 tgcccatcaa aggccggatg aggccgatct gctcagcgct caagccgccg aacaacgaat
     3361 aggcctgtaa cgccgccgtg tctaccatat cttgtatact gtaccatact agcagcacag
     3421 tgacaatgat cattttatct cgtatagttg catgtatggg agacggcata tgcacgccat
     3481 aaggcagcgg tcggaagccg ccaaggtacg ccgtacgttc tactcgataa gcctgcttat
     3541 cgtcatcgtc gtcttcatcg tattcactac ctcccacttc acctcgctca agcgccagaa
     3601 cgacgagagt ttcaagaggc tcgaagaact catggtcacc atgaccaagg tgtacctgaa
     3661 ggataccgtg agccgcacct tggcggatat agacctcgag cggaagatga tcaggctgga
     3721 tctgcaggtg gccgctggag acgccgaggc cgagagcaga gccgacgaac tggtcaaggc
     3781 tcgctccatc gtcaggatca gggagactcg ccttatagaa gacggctacc tctgggccag
     3841 cgagctgctc accccggccg gcggccggga ctacgccagg cgcatcgcac acgggcgcta
     3901 tcccgattcc gagggtacgc tcctgtcgac cgaagcttcg tcgggtttga acgcctcgca
     3961 ataccgggag gaactcgacg gcctattggc ggacggagag gtctactata cctactacac
     4021 gccaaaaaac ggcgagccgc taccgtcgcg gatgctggtc tacgccgccc tatatc
//
LOCUS       DAOOLN010000027         3999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_27182_length_3999_cov_2.663032, whole genome shotgun sequence.
ACCESSION   DAOOLN010000027 DAOOLN010000000
VERSION     DAOOLN010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27182_length_3999_cov_2.663032"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..1328
                     /locus_tag="PLW80_00645"
     CDS             249..1328
                     /locus_tag="PLW80_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HPB65037.1"
                     /translation="MRLTLTENQNAIAPVFLQSRFWADFKVEAGWSYVRYDARLDDGP
                     YAGSIFTLTVMERGLGVGFRFAYVPHGPSVALEPAGRTELLVALAKALRGLVSGSCLF
                     IRFDPAWYEAEDASGEASSDRLATPPRPVFSAPLRKASDVQPPDSVVLDIARPDDDLL
                     AGMKPKWRYNIRLAEKKGVTVASEGAGSLGEFYALYEATAARDRIAIHPASYYERLFG
                     LEVAGSAEPKPDIRLWMARHEGLALAGIVTVFYGDEATYLYGASGDEHRNLMPAYALQ
                     WAAVKAARDAGCRSYDFYGIPPTDDPKHAMSGLYRFKTGFGGEIRHYAGAWDYVLRPA
                     AYAAFRAAERARLFWHKALRKRLGR"
     gene            complement(1501..3183)
                     /locus_tag="PLW80_00650"
     CDS             complement(1501..3183)
                     /locus_tag="PLW80_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65038.1"
                     /translation="MAKRLLIRDLTLRDGQQSQFATRMNQDQVDRVLPFYREARFYAM
                     EVWGGAVPDSVMRFLNESPWTRLETIRDGVAGASKLTALSRGRNLFGYNPYPDSVIEG
                     FCRNSVRSGIDIMRVFDALNDVDNMTSTIRFVKENGGMADCAVCYTVDPKFTFGDRVG
                     AFFKGKKIPGTLFGVPYFVRKAKELAAAGADMITIKDMAGLVDPATSAELIKALKAEV
                     GVPIDLHTHCTPGYGTASVLMAMVSGVDVVDTVVMNFSGGPAAPAFEIVQLLADRLGI
                     DTGVDLAAVAAINRELGGIRAELSKFDQFKRLPPDIDLTAYEPSPELRSLFDGAAEAA
                     TAGRIDETLRLCQAIEAAFNYPEPDEIVKRAQIPGGMYTNMMSQLKEAKLEQHLEEVL
                     QIVPQVRLDAGVPPLVTPTSQIVGVQAVNCIVDKAQGKPFYSNVSKNFVELVKGSYGR
                     TPFPVDPAFREKVAGVRDEVPYDTSKYKKQDNPELADCGGLRLALNEKEELLLELFPS
                     VADKFLKGRRQAEWQAMEAARLAALPPEPDPDEGARYPSKFWEEELQLAIEG"
     assembly_gap    3409..3418
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3419..>3999)
                     /locus_tag="PLW80_00655"
     CDS             complement(<3419..>3999)
                     /locus_tag="PLW80_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPB65039.1"
                     /translation="VRSIERALDILDCFKPGRLELTLTEIATGIELAMSTTSRIVATL
                     EKRHYLSRNPDTQRYSLGSRISQIGALGLSNMDLRRVALPFMKELNALYNEGVSLYIE
                     RDNERICIERVESTLPLRRVINVGDRHPLTRGAAGRVLLAYMPADRRQALLSEDPFTT
                     EESLAELRHGGYTVSLGEREEGVTSIAAPIQDA"
BASE COUNT          686 a         1229 c         1316 g          758 t
ORIGIN      
        1 gcccacgaca gtcaccgaga agaacgcgtc cacatcgtgg ccaagggcaa tacgccgatg
       61 gtcggagtca aggccctgac ggctgatggc aagaacctcg ctaccacgaa gattactgac
      121 ggcgaggtgg gctgagcttc cgataggctc agcggctgaa ataagcctcg tgatgtcgga
      181 aatgtatgtc ggggcggcga atcaaggctg atttcgccgc cccttcgtct taaaaaggat
      241 acatgcagat gcgcctgacc ctaactgaga atcaaaacgc catcgctccc gtatttctcc
      301 agagtcgatt ctgggcggat ttcaaggtgg aggctggttg gagctacgtc cgctacgacg
      361 cgcggctcga cgacgggcca tacgccggct cgatcttcac gctgaccgtg atggaacgcg
      421 gcctcggggt cggtttccgt ttcgcctacg taccgcacgg accgtccgtg gccttggagc
      481 cggccggacg gacggagctt ctcgtggccc tggccaaggc cttgcgcggc ctcgtatcgg
      541 gatcctgcct gttcatacgc ttcgatccgg cctggtacga ggccgaggac gcttcaggcg
      601 aagcgtcctc ggacagactc gctacgcctc ctaggccggt ctttagcgcg ccgctgcgca
      661 aggcctccga cgtgcagccg cctgacagcg tcgtcctcga catcgcgagg cctgacgacg
      721 acctgctcgc cggcatgaag ccaaagtggc gctacaatat caggctcgcg gagaagaaag
      781 gcgtgacggt agcctcggag ggagccggct cgctgggaga gttctacgcg ctctacgagg
      841 cgacggcggc acgcgatcgg atagccatcc atcccgcctc gtattacgag cgtctgttcg
      901 gactcgaggt cgcgggctcg gccgaaccga agccggatat caggctgtgg atggcgcgcc
      961 acgaaggact ggccttggcc ggtatcgtca ccgtcttcta cggcgacgag gccacctacc
     1021 tgtacggcgc gtcgggcgac gagcatcgca acctgatgcc cgcctacgcg ctccaatggg
     1081 ccgccgtcaa ggctgccagg gacgcggggt gccgtagcta cgacttctac ggtatacccc
     1141 cgaccgacga cccgaagcat gcgatgtccg gcctctatcg cttcaagacc ggcttcggtg
     1201 gcgagatccg ccactatgcg ggcgcttggg actacgtgct caggccggcg gcctacgcgg
     1261 cgttcagggc ggccgagcga gcgagactgt tctggcataa agcgcttagg aagcggctgg
     1321 ggagatgata cggagaccga tcgcctctat gccggtatct ggatgaaagc gtccacacta
     1381 cgaggtttcg atgtgtcctg gattagttcg aaggtaccgt cgaccatagg gaatcgctag
     1441 cggggattga atggaggagg cggcctttcg accgcctcct tcgttctccg cctatcgcta
     1501 tcagccctcg atggccagct gcagttcctc ttcccagaat ttggacggat accgcgcgcc
     1561 ttcgtccgga tccggttcag gcggcaaggc ggctagtcgc gctgcctcca tcgcctgcca
     1621 ttcggcctgc ctccgaccct tcaggaattt gtcggccacg ctcgggaaca gctcgaggag
     1681 gagttcctcc ttctcgttga gcgcgagcct gaggccaccg cagtcggcca gctcgggatt
     1741 gtcctgcttc ttgtacttgc tcgtgtcgta gggcacctcg tctcgcacgc cggctacctt
     1801 ctcgcggaag gccgggtcga ccgggaaggg cgtgcgtccg taggagccct tgaccagctc
     1861 gacgaagttc ttggagacgt tcgaatagaa gggcttgccc tgggccttgt cgacgatgca
     1921 gttgaccgcc tgcacgccga cgatctggct ggtcggggtg acgagcggcg gtacgccggc
     1981 gtcgagcctg acctgcggca cgatctggag cacttcctcg aggtgctgct cgagcttggc
     2041 ttccttgagc tgcgacatca tgttcgtgta catgccgccg gggatctggg cgcgtttgac
     2101 gatctcgtcg ggctcggggt agttgaacgc ggcctcgatg gcctggcaga gcctgagcgt
     2161 ctcgtctatc ctaccggccg tagcggcctc ggccgccccg tcgaacaggg aacgcaactc
     2221 cgggctcggt tcgtaggcgg tcaggtcaat atccgggggt aggcgcttga actggtcgaa
     2281 tttggacagc tcggcgcgta tgccgcctag ctcgcggttg atagccgcta cggccgccag
     2341 gtcgacgccg gtatcgatgc cgagccggtc ggccaggagc tggacgatct cgaaggccgg
     2401 cgccgcgggg cctccggaga aattcatgac cacggtgtcg acgacgtcga cgcccgagac
     2461 catggccatc aggacgctcg ccgtcccgta accgggcgta cagtgggtgt gcagatctat
     2521 gggcacgccg acctcggcct tgagggcctt gatgagctcg gcgctggtcg cggggtcgac
     2581 gaggccggcc atgtccttga tggtgatcat gtcggcgccg gcggccgcta gctccttggc
     2641 cttgcgcacg aaatagggca cgccgaatag cgtgccgggt atcttctttc ccttgaagaa
     2701 ggcgccgacg cggtccccga aggtgaactt cgggtcgacg gtatagcaga ccgcgcagtc
     2761 ggccatgccg ccgttctcct tgacgaagcg tatggtactc gtcatgttgt cgacgtcgtt
     2821 cagcgcgtcg aacacgcgca tgatatcgat gcccgacctc acggagttcc tgcagaagcc
     2881 ctcgatgacg gagtcggggt aggggttgta gccgaacagg ttgcgcccgc gggatagggc
     2941 ggtcagcttg gaggcgccgg ccacgccgtc ccggatggtc tcgagcctgg tccaggggct
     3001 ctcgttcagg aagcgcatca ccgaatcggg gactgcgccg ccccagactt ccatggcgta
     3061 gaatcgggcc tcgcgataga acggcaggac gcggtcgacc tggtcctggt tcattcgggt
     3121 ggcgaactgg gattgctggc cgtcgcgcag ggtcaggtcg cgaatcaaga gacgcttagc
     3181 catcgtatgc tccgtagtat catgatggtc gcgagtcgaa aggacgcgac cgtccttcca
     3241 gtatacagga tgcccggacg atgatccagc cgatcggacc gctatcgccc gagcgcgttc
     3301 gatatgagct cggcgcagcg cttgaccttg gctatcttgt cggagaagcc cgagccctcg
     3361 aagcggaccg acgggcccga catcgacagg gcggcgatca cggcgcacnn nnnnnnnngg
     3421 gcgtcctgga tgggcgccgc gatagaggtg acaccttcct cccgctcgcc gaggctgacc
     3481 gtgtagccgc catggcgcag ctccgcgagg ctctcctcgg tagtgaacgg atcttcggac
     3541 agtagggcct gacgacgatc agccggcatg taggccagga gcaccctgcc ggcggctccc
     3601 ctggtcaggg gatggcggtc gccaacgttg atcactcgcc tgagcggtag cgtactttca
     3661 acccgctcga tgcagatccg ctcgttgtcg cgttctatat agaggctgac accctcgttg
     3721 tagagcgcgt tcagctcctt catgaacggc agggccaccc tgcgcaggtc catgttggat
     3781 aaaccgagcg ctccgatctg ggatatcctc gagccgagcg agtagcgctg cgtatcgggg
     3841 ttcctggaca ggtagtggcg cttctcgaga gtcgcgacga tgcgcgatgt cgtgctcatc
     3901 gccagctcga tgccggtggc gatttcagtg agcgtcagct ccagcctgcc cggcttgaag
     3961 cagtccagga tgtccagcgc tcgctcgatg gatcggacg
//
LOCUS       DAOOLN010000028         3993 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_27255_length_3993_cov_3.093195, whole genome shotgun sequence.
ACCESSION   DAOOLN010000028 DAOOLN010000000
VERSION     DAOOLN010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27255_length_3993_cov_3.093195"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PLW80_00660"
     CDS             complement(<1..844)
                     /locus_tag="PLW80_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB65040.1"
                     /translation="MPKPPPLLSLQHISKSFYGNPVLEDVSFDVGPGEIVGLVGENGA
                     GKTTLMRILFGMPVIAETGGYGGRIELDGAQVSFKTPFDALDAGIGMVHQEFSLIPGF
                     STTENIVLNREPMKANAACEVFGDRMSVLNRTTMRSVAEKAIERLGVKLDPETLVSEM
                     PVGHKQFTEIAREINRENVRLLVLNEPTAVLTESEAEILLEALRRLAAQGISIIFISH
                     RLHEITKVADTIVVLRDGKVVKEAPSEGTSVRDIAAWMVGRSVEAKRPDLATRSFGEE
                     ALRVE"
     gene            complement(1020..2252)
                     /locus_tag="PLW80_00665"
     CDS             complement(1020..2252)
                     /locus_tag="PLW80_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HPB65041.1"
                     /translation="MKLSRISAAILFAAVIAFAGCQKAEKPAAPAEKAKAAFHIGVVT
                     GTVSQSEDDLRGAEELIKRYGKVSDGGMIQHITYPDNFMDQQETTISQIVALADDPLM
                     KAIVMNQAVPGAAEGFKRVREKRSDILLFAGEPHEDPLVIEAASDLALNSDFISRGYT
                     IIWAAKELGAKTFVHISFPRHMSYETLGLRRQIMEQACKDLGLTFAFETAPDPTSDVG
                     VAGAQQFILEKVPAWIEKYGKETAFFCTNDAHTEPLLKQLLQYGGYFVEADLPSPLMG
                     YPGALGVDLTAEAGNFPAILKKVEQAVVDKGGAGRFGTWAYSYGFTVTAGLGEFAKNV
                     IEGKTTKDSLDGLFEAFAVYTPGAQWNGGYYIDANTGVRAKNHLLVYMDTYILGKGYL
                     PTTKQTVPEKYFSIKFKK"
     gene            2339..3475
                     /locus_tag="PLW80_00670"
     CDS             2339..3475
                     /locus_tag="PLW80_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPB65042.1"
                     /translation="MLYRRFPKIPDLDISALGLGCMRLPTRPDDQAAIDEDAADALLS
                     DALAAGVNYLDTAWPYHKERSEPWLGRAVERLGCRDEVLIATKAPTWLIEEPGDWERF
                     LAEQLLRLRVDRIDFYLAHALNRKRWRTMLETGGLEFLAKAKADGRIGHVGFSFHDDL
                     DSFKEIVDGWPDWEFCQVQYNYIDEEYQAGDAGIRYAAERGLGVIVMEPLRGGVLADV
                     PDEVRKAFASYPIPRMAAEWALRHVLDRQEVVTALSGMGASSQLWENAAVASSARANS
                     ITTPERAAIDAARDWFRSRMATPCTACGYCRPCPAGVSIQGVFELWNDASMFGDREGA
                     RRRYATELAGHGADACVACGECVPKCPQGIDIPAMLRRAAEALG"
     gene            3497..>3993
                     /locus_tag="PLW80_00675"
     CDS             3497..>3993
                     /locus_tag="PLW80_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65043.1"
                     /translation="MRRSNRVIARVFSLLVAAILPAILVFWVVISGLFVEAAATNANR
                     TKAALMNQADRLAFDLDLELTLLDSCLATAMARLTPSDGAASWEEPIARGVEAYKAAS
                     RWPLLVASVVLVEEGEAFGDPSADDAATHMIRLAPGGSFESGGRALVAILDQGVLAGT
                     VIPILA"
BASE COUNT          656 a         1264 c         1352 g          721 t
ORIGIN      
        1 gctcgacgcg aagcgcttcc tcgccgaagg agcgggtcgc caggtcgggg cgcttcgcct
       61 cgaccgagcg gccgaccatc caggcggcga tatcgcgtac cgaggtaccc tcggacggcg
      121 cttccttgac gaccttgccg tcgcgcagca cgacgatggt atcggcgacc ttggtgatct
      181 cgtgcaggcg gtgcgatatg aagatgatcg agatgccctg ggcggccagc cgcctgaggg
      241 cctcgagcag gatctcggcc tcgctctcgg tcagcaccgc ggtcggctcg ttgagcacga
      301 ggagcctgac gttttcgcgg ttgatctcgc gcgcgatctc ggtgaactgc ttgtggccta
      361 ccggcatctc ggagacgagg gtctccgggt cgagcttgac gccgagccgc tcgatggcct
      421 tctccgcaac ggatcgcatc gtcgtccggt tgagcacgct catccgatcg ccgaagacct
      481 cgcaggcggc gttggccttc ataggctctc ggttgagcac gatgttctcc gtcgtgctga
      541 agcccggtat cagggagaat tcctggtgaa ccatgccgat gccggcatcg agcgcgtcga
      601 acggcgtttt gaaggagacc tgagccccgt ccagctcgat ccgaccgccg tagccgccgg
      661 tctcagctat cacgggcatg ccgaacagga tgcgcatcag cgtggtcttg ccggcgccgt
      721 tctcgccgac gagaccgacg atctcgcccg gcccgacgtc gaacgagacg tcctccagga
      781 ccgggtttcc gtagaagctc ttcgatatat gctgcaagga aagcaagggt ggtggtttgg
      841 gcatgtgtcc tacctttttt cgtgcgaggt cggccgtcct gggacgtcga taccgctggc
      901 cctgactgga tcggcgggct agcgagcccg cgcgcgagag gcatcataat cgatgccggc
      961 ggtattgtca aaaaaaatga gccgccccgg agggcggctc gatacggcgg gaaagggaat
     1021 tacttcttga acttgatcga gaagtacttc tcgggcaccg tctgcttggt ggtgggcagg
     1081 tagcccttac cgaggatgta ggtgtccatg tagacgagga ggtggttctt ggcgcggacg
     1141 ccggtgttgg cgtcgatgta gtagccgccg ttccactggg cgcccggcgt atagacggcg
     1201 aaggcctcga acaagccgtc caggctgtcc ttggtggtct tgccctcgat cacgttcttg
     1261 gcgaactcgc cgaggccggc ggtgacggtg aagccgtagc tataggccca ggtgccgaaa
     1321 cggccggcgc cgcccttatc gaccacggcc tgctcgacct tcttgaggat ggcggggaag
     1381 ttccctgcct cggcggtcag gtcgacgccg agcgctcccg ggtagcccat gagcggcgac
     1441 ggcaggtcgg cctcgacgaa gtatccgccg tactggagca actgcttgag gagcggctcg
     1501 gtgtgggcat cgttcgtgca gaagaaggcg gtctccttgc cgtacttctc gatccaggcc
     1561 gggaccttct cgaggatgaa ctgctgggcg ccggccacgc cgacgtcgga ggtcgggtcg
     1621 ggggcggtct cgaacgcgaa ggtcaggccg agatccttac aggcctgctc catgatctgc
     1681 ctgcgcagac cgagagtctc gtacgacata tggcgcggga aggagatgtg gacgaaggtc
     1741 ttggcgccaa gctcctttgc ggcccagatg atcgtatagc cgcggctaat gaagtcgctg
     1801 ttcagggcga ggtcggaggc cgcctcgatc acgagcggat cctcgtgggg ctcaccggcg
     1861 aagagcagga tgtcggagcg cttctcgcgc acgcgcttga agccctcggc tgcgccgggt
     1921 acggcctggt tcatcacgat agccttcatg agcggatcgt cggccagggc tacgatctgg
     1981 gagatcgtgg tctcctgctg gtccatgaag ttgtcgggat aggtaatatg ctggatcatg
     2041 ccgccgtcgg acaccttgcc gtagcgcttg atcagctctt cggcgccgcg caggtcgtcc
     2101 tcggactgcg ataccgtacc ggtcactacg ccgatatgga aggccgcctt ggccttctcg
     2161 gcgggagccg cgggcttctc ggccttctgg caacccgcga atgcgatgac ggccgcgaac
     2221 aggatcgcgg cggagatgcg ggacagtttc atgaatcctc cttgttcgtc ggcgaaccga
     2281 cgatccgacc attgtaaacc attgaaggga tacgtcaagg gtatgctata cttcgttcat
     2341 gctgtatcga cgattcccca agatccccga cctggacata tcggcgctcg gcctcggctg
     2401 catgcgccta ccgacccgcc ctgacgacca ggcggccatc gacgaggatg cggcggacgc
     2461 cctgttgtcc gacgcgctcg cggccggcgt caactatctc gatacggcct ggccctatca
     2521 taaggaacgg agcgagccct ggctcggccg ggccgtcgag cgcctcggct gccgcgacga
     2581 ggtactcatc gcgacgaagg ccccgacctg gctgatcgag gagcccggcg actgggaacg
     2641 tttcctcgcg gaacagctct tgcggctgcg cgttgaccgg atcgactttt acctggcgca
     2701 cgcgctcaac cgcaagcgct ggcggacgat gctcgagacc ggggggctgg agttcctggc
     2761 caaggccaag gccgacggaa ggataggcca cgtcggcttc tcgttccacg acgacctcga
     2821 ttcgttcaag gaaatcgtgg atggctggcc cgattgggaa ttctgccagg tccagtacaa
     2881 ttacatcgac gaggaatacc aagcgggcga cgctggcatc cgctacgccg ccgagcgggg
     2941 cttaggcgtc attgtgatgg aaccgctacg cggcggtgtg ctggccgacg tgccggacga
     3001 ggtgcgcaag gccttcgcgt cctatcctat cccgaggatg gccgccgaat gggcgctccg
     3061 ccacgtgctg gaccggcagg aggtcgtgac ggcgctctcc ggcatgggcg cctcgtcgca
     3121 gctctgggag aacgcggcgg tagcctcctc ggcgcgcgcc aactcgatca cgacgcccga
     3181 gcgcgcggct atcgacgccg cgcgggactg gttccgctcg cgcatggcta cgccgtgcac
     3241 cgcctgcggg tactgccgcc cctgccccgc cggcgtgtcg atccagggcg tgttcgagct
     3301 ctggaacgat gcctcgatgt tcggcgaccg cgagggcgcc cgcaggcggt acgcgaccga
     3361 actagcgggg cacggcgccg acgcctgcgt cgcgtgcggc gagtgcgtcc cgaagtgccc
     3421 tcagggcata gacatcccgg ccatgctcag gcgggcggcc gaggccctcg gctgatcgta
     3481 gcccggagga ggccggatga ggaggagcaa tcgggtcatc gcgcgcgtat tctcgctcct
     3541 ggtagcggcg atactacccg cgatactcgt cttctgggtt gtcataagcg gcctgttcgt
     3601 ggaagcggcc gctacgaacg cgaacaggac aaaggcggcg ctgatgaacc aggccgaccg
     3661 cctggccttc gacctcgacc ttgagctgac cctgctcgac tcgtgcctcg ccacagccat
     3721 ggcccggctc acgccgtcgg acggcgccgc ctcctgggaa gagccgatcg cacgcggcgt
     3781 cgaggcgtat aaggcagcct cgcgttggcc gctcctcgtg gcctccgtcg tcctggtcga
     3841 agaaggcgaa gccttcggcg atccgagcgc cgatgacgcg gcgacccaca tgatccgcct
     3901 agcgcccggg ggctccttcg agagcggagg acgcgcgctc gtggccatcc tcgaccaggg
     3961 cgtgctcgcc ggtaccgtca tccccatcct ggc
//
LOCUS       DAOOLN010000029         3952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_27629_length_3952_cov_3.106492, whole genome shotgun sequence.
ACCESSION   DAOOLN010000029 DAOOLN010000000
VERSION     DAOOLN010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27629_length_3952_cov_3.106492"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1695)
                     /locus_tag="PLW80_00680"
     CDS             complement(<1..1695)
                     /locus_tag="PLW80_00680"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HPB65044.1"
                     /translation="MEVTTDVYRFIVRPKERPGGRRRHEACPLAADARALGLSSVRAI
                     ERAALYFVRGTMAPKELCLLGRFLFSDPLVEDCSWSRVGDEEPDEAAAPGDDGGDDGG
                     DDGDAMRRVEAHLVETALRPGVTDPVADEAMRAAAELGVAGVEAVSSGEAFFIHGDGL
                     SDEELSLLAERLLCNAVIQRWAVGAIEPAWPDEAAGSGAVERFELAAMDDEALLALSA
                     ERRAALDLAEMAAIRGYYRREGRACTDAEFETIAQTWSEHCVHKTFKARIEVRADGEA
                     AAPYPPVVDNLLKSYIKRATDEIAAPWVLSAFVDNAGVIEFDGEHELSFKVETHNHPS
                     AVEPFGGANTGVGGVIRDVMGVSARPIAATDVLCFGPPDASPEDLPAASLHPRRVASG
                     VIAGVQDYGNKMGVPTVNGGIHYDPGYAANPLVYCGCAGIAPRGRHPRSQAAGDRVVV
                     LGGRTGRDGLRGATFSSMVMDSSTGELSGASVQIGAPIVQKKVSEALVLARDAGLYTA
                     VTDCGAGGLSSAVGEMAAELGADVDLARTPLKYPGLAPWEIWLSEAQERMVVAVPPA"
     gene            complement(1801..2352)
                     /locus_tag="PLW80_00685"
     CDS             complement(1801..2352)
                     /locus_tag="PLW80_00685"
                     /inference="COORDINATES: protein motif:HMM:NF018813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HPB65045.1"
                     /translation="MAIGATVCKVTLSVSDLDRHYYAEHRLTVSREPSEPDERFMARV
                     LAFALNADDGLAFARGQAEADEPELWRRGLDGGIGLWIELGLPDPKRLRKASARADEV
                     LLYLYHGRQASLWWEQHGAELSALPKLRVVEIDGASVRSLAAMAAKSLELSCLVQDGQ
                     AWIDDGDTRLELELRALKERSDR"
     gene            2456..2866
                     /locus_tag="PLW80_00690"
     CDS             2456..2866
                     /locus_tag="PLW80_00690"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HPB65046.1"
                     /translation="MVCNAAGALPRLIVVAAVIELDGRILCAQRKAGGELSLKWEFPG
                     GKVEPGETRERALEREIDEELGLRIRTGPLLLTVRHRYAAFAVTMHAYRATILEGQPE
                     LREHPAIRWLRPDELGTLDWAPADVPVAKKMIGL"
     gene            complement(2879..3478)
                     /locus_tag="PLW80_00695"
     CDS             complement(2879..3478)
                     /locus_tag="PLW80_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HPB65047.1"
                     /translation="MRTRTPLIYFREVQSHRYQAIWPVIVLEDFQERLCVRAAIDPAY
                     AELRPGLSFSDIAESPLDLRRYAWTQTRHRLHQGAFRELVVSAYEERCAICRLNHPEL
                     LDAAHIIPDSDERGTPIVANGLSLCKIHHAAYDQGILGIDADYRVHIREDILIERDGP
                     MLKHGIQELDGSTLIVPRHADERPDRDRLAERYSRFFVA"
BASE COUNT          658 a         1322 c         1310 g          662 t
ORIGIN      
        1 ggcgggcggt accgctacga ccatgcgctc ctgggcctcg gacagccata tctcccacgg
       61 cgccaggcca gggtacttga gcggcgtgcg ggccaggtcg acgtcggcgc cgagctcggc
      121 cgccatctcg ccgaccgccg acgagaggcc tccggcgccg cagtcggtaa cggccgtata
      181 gagcccggcg tcgcgcgcca ggacgagggc ctcggatacc ttcttctgta cgataggcgc
      241 gccgatctgc accgacgccc ccgatagctc tccggtgctc gagtccatca ccatcgacga
      301 gaaagtcgcg ccgcgtaggc cgtcgcggcc ggtgcggccg cccagtacga ccacgcgatc
      361 gccggccgcc tgcgagcgcg gatgtctgcc gcgcggcgcg atgccggcgc aaccgcagta
      421 tacgagcggg ttggccgcat agccggggtc gtagtggatg ccaccgttga ccgtcggtac
      481 gcccatcttg ttgccgtagt cctggacgcc ggctatcacg ccggaggcca cgcgccgcgg
      541 atgcaggctg gcggccggca ggtcctccgg cgacgcgtcc ggcgggccga agcacagcac
      601 gtcggtagct gctatcggcc tggccgacac gcccatcacg tcgcgtatga cgccgccgac
      661 gccggtgttg gcgccgccga acggctcgac cgcggacggg tggttgtgcg tctccacctt
      721 gaacgacagc tcgtgctcgc cgtcgaactc gatgacgccg gcgttgtcga cgaaggccga
      781 taggacccag ggcgcggcga tctcgtcggt ggcccgtttg atgtagctct tgagtaggtt
      841 gtcgacgacg ggcgggtagg gcgccgccgc ctcgccgtcg gcccgtacct cgattcgggc
      901 cttgaaggtc ttgtgcacgc agtgctcgct ccaggtctgc gctatcgtct cgaactcggc
      961 gtcggtgcag gcgcggccct cgcggcggta gtagccgcgt atagccgcca tctcggccag
     1021 gtcgagggct gcccggcgct cggcggacag cgcgagcagc gcctcgtcgt ccatcgcggc
     1081 cagctcgaag cgctccaccg cgccggagcc ggccgcctcg tcgggccagg ccggttcgat
     1141 cgcgccgacg gcccagcgct ggattaccgc gttgcacagg aggcgctcgg cgagcaggga
     1201 cagctcctcg tcgctcaggc cgtcgccgtg tataaaaaac gcctcgcccg acgatacggc
     1261 ctcgacgccg gccacgccga gctcggcggc cgctcgcatc gcctcgtcgg cgaccgggtc
     1321 ggtcacgccc ggccggagcg ccgtctctac gagatgggcc tcgacgcgcc tcatcgcgtc
     1381 cccgtcgtct cctccatcgt ctcctccatc gtcccccggg gccgccgcct catcgggctc
     1441 ctcgtctccg acacgcgacc atgagcagtc ctcgacgagg gggtcgctaa aaagaaagcg
     1501 cccaaggagg cagagctcct tgggcgccat cgtgccgcgc acgaagtata gggccgcgcg
     1561 ttctatcgcg cggacgctcg acaagccgag cgcgcgcgcg tccgcggcga gcgggcaggc
     1621 ctcgtgcctg cgtctcccgc ccggccgttc cttgggcctg acgataaacc ggtaaacgtc
     1681 cgtcgtgact tccatgagga ttactactgc ctatcgcgta gcgtcgtcaa gtaccccgca
     1741 ggatgttgtt tgcgtctagc gatcggccga gcgacgaggc ggcggcggta agctgcccga
     1801 tcagcggtcg gagcgctcct tgagggcgcg cagctccagc tcgagccgcg tatcgccgtc
     1861 gtcgatccag gcctggccgt cctggacgag gcaggacagc tcgagcgact tggcggccat
     1921 cgcggccagg ctacggacgc tcgcgccgtc aatctcgacg acgcgtagct tcggcaaggc
     1981 cgacagctcc gcgccgtgct gctcccacca gagcgaggcc tggcggccgt ggtacaggta
     2041 gaggagcacc tcgtccgccc gggccgaggc cttgcgcagg cgcttgggat cgggcaggcc
     2101 gagctctatc cagaggccta tcccgccgtc gaggccgcgg cgccacagct ccggctcgtc
     2161 ggcctccgcc tgtcccctgg cgaaggccag accatcgtcg gcgttcagcg cgaaggccag
     2221 gacgcgcgcc atgaagcgct cgtccggctc cgacggctcg cgcgatacgg tcaacctatg
     2281 ctcggcgtag taatgccggt ctaggtccga tacactcaaa gtaaccttgc agactgtcgc
     2341 tccgatggcc atggactatc ctccccgggt cggtaaggag tatggcgagg atggctgggc
     2401 agcgcaatcc gaggccgcat ggacgcgccg gcgttccggg tgtacgctgg cgaccatggt
     2461 atgtaacgcc gccggagccc ttccgcgact catagtagta gcggccgtca tcgagctgga
     2521 cggcaggatc ctatgcgctc agcgaaaggc cggcggcgag ctgtcgctga aatgggagtt
     2581 ccccggcggc aaggtagagc cgggagagac gcgcgagcga gcgctcgagc gcgagatcga
     2641 cgaggaactc ggcctccgta tccggacggg acccttgctc ctgaccgttc gccaccggta
     2701 cgccgccttc gccgtaacca tgcacgcgta tcgggctact atcctcgagg ggcagccgga
     2761 attgcgcgag caccctgcca tacgatggct acgtcccgac gagctcggaa ccctcgactg
     2821 ggcgccagcc gacgtacccg tcgcgaagaa gatgataggg ctgtaggcac gaacgacctt
     2881 aagcgacgaa gaacctcgag tatcgttcgg ccagccggtc tcggtcgggg cgctcatccg
     2941 cgtgccgagg tactatgagc gtggaaccgt cgagctcctg gatgccgtgc ttgagcatcg
     3001 ggccgtcgcg ttctatgaga atatcctccc tgatatggac gcgataatcg gcgtcgatgc
     3061 cgaggatgcc ttgatcatag gcggcatggt gtatcttgca cagcgacagc ccgttggcca
     3121 cgatcggcgt gcctcgctcg tccgaatcgg ggatgatatg cgccgcatcg agcagctcgg
     3181 gatggttcag ccggcaaatg gcgcagcgtt cttcgtaggc agaaacgacc agctcgcgga
     3241 acgcgccttg atggagccta tgcctcgtct gggtccaggc gtagcgccgt aggtccagcg
     3301 gcgattcggc gatatcggag aatgataggc cgggacgcag ttcggcgtac gcgggatcta
     3361 tcgcggcgcg tacgcacaaa cgctcctgga agtcttcgag gacgatgacg ggccagatcg
     3421 cctgatagcg atggctctgc acttccctga aatagatcag cggcgtcctc gttctcattg
     3481 cgttacgaag gcctacgtta tcccgatgat ccgggtctat cccgcgatac gcgtaggtca
     3541 gaagtccgtc ggggccgata tcgtcaagtt cataggggcc ttgattcgag gtggttatcg
     3601 aaatcggcgt cgtgaacccg gcggggaacc agatgcccgt ctgcccttta agggtgacgt
     3661 tgcggccctc gactaagatc ccggcattga gctcgcgacc gctgaagata ccgccattcc
     3721 aacgatcgcg atcctggagc catcggaata ccgtgagcct gatcacagtc tcatcaagca
     3781 cgacgagcct ccatgaacct tatcccggac aatccattgc gtaactcgct gacggacagc
     3841 cattggaaga gcgccatcgg gcggcggtag ccatgtatcc tagccccgcc ccaactatat
     3901 ggcaagctcg cgttaaggat cgaagcgtca ggcggctgat cgagtagcac ct
//
LOCUS       DAOOLN010000030         3900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_28127_length_3900_cov_3.675683, whole genome shotgun sequence.
ACCESSION   DAOOLN010000030 DAOOLN010000000
VERSION     DAOOLN010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28127_length_3900_cov_3.675683"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1235)
                     /locus_tag="PLW80_00700"
     CDS             complement(114..1235)
                     /locus_tag="PLW80_00700"
                     /inference="COORDINATES: protein motif:HMM:NF025608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HPB65048.1"
                     /translation="MGDERNGYMLSGARARRGYDWWWHSLVAMSEKTGERRPFFIEYY
                     VINPGFGDAEPVAGQLPANKKAGRKPCYAMIKAGCWGAAAKAQLHNLYPVAAFSASRD
                     GMDVRIGDHVATETRLKGSVSLSAADAAAHPEYMSDSGEMSWDLEAEKILRFSVGWGT
                     SAFFRKLDAFNMYWHVAGMRTRYKGVITFNGERFIVSPDDSFGYQDKNWGRDYTSPWV
                     WLNCNNLRSRKTGKLLERSSLDVGGAQPVAFGISLPRRLLIALHYEGAFYEWNFSKFW
                     TGSKQVFDCPVRDDRVEWNIDAWHGTAKVEIRFACPRDTMLNVNYENPDGEKKHNELW
                     NGGYASGTIRLLERRGGEWVEVDVLEGELGGCEYGEYDR"
     gene            1370..3451
                     /locus_tag="PLW80_00705"
     CDS             1370..3451
                     /locus_tag="PLW80_00705"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPB65049.1"
                     /translation="MATVTIERLPALSVGASVPLPNGYVDSGYAEDCASLPRDYGTES
                     WEARPEVIEIRRAVFAALDRLEGETSIASRFRGRPVLVKPNLVTVYQDMGMRRGSYPE
                     TTDPRVLDAVVLWLGRFASTIRIVESSGRGAPTRGSFRVSGIDRLARHRGCDLVALEE
                     TPVDRYLLPKASAQREILVPEAFSAVVRGEAAYVSVPKLKTNLYTGVTLGFKNAMGVI
                     PYNLRQRNHHYAINRKLVEMLYLFKPDLVVIDGVVGGEGECPAPVDPVDSRVIIAGDH
                     PVETDRVATRLMGFDPGSIELMRVADELGFGDATGARVLANGFDAPARDQGGLSSARS
                     AIPDLGGAVPVRFCPADWSLTSERVRGAFPGILVLIGIDRGGLEGKSGPELVEAMQAS
                     CRGGCVATTRFAFSMFEAEGVRPRRPVALIIGCGLERDGRRLWFDADGKPYAEDDIHA
                     LRMRKAVIGSCTRVLAPLATRFVDGCMVLPNAPHVVLHELSGTPCRVMSLRNRNLFPM
                     LAATLGQRAARRRLIESGKRLDVPLRLNDEPIKTRELAPGEEKRDRVAWPMPPIEPEE
                     MCRLLDFEDDATLASLRGIIVFRIKERALWRAKQVLATIVTLGPLVLGALAAIEVSRF
                     GLGTAAWLAIFGAVEAIHLAELPAALRAWKKEAERRNAPWSGARTVRAVAATLSCGYP
                     SWVPRKLGVFD"
     gene            3541..>3900
                     /locus_tag="PLW80_00710"
     CDS             3541..>3900
                     /locus_tag="PLW80_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB65050.1"
                     /translation="MISPIQHNGIFLGLSSFQALAMFRRGIFYTFLAVYLRSHLGLSV
                     TETTLFETVPMALNVLFQTLVWGRLTDRLQKRRTLIIIGELMASVGHVAMWYFHAVAP
                     TPRAAGYVIIAGLTVIEA"
BASE COUNT          621 a         1329 c         1282 g          668 t
ORIGIN      
        1 ctcgtcatcg gctcgctgtc cagactgacc acggcggagc aatccgcctt cgtcgtcgta
       61 gccgagaacc tcgacgaggc tctcggcgtc gccgaccgga cagtctacga gccctagcgg
      121 tcatattctc cgtactcgca gccgcccagc tcgccttcga ggacgtcgac ctcgacccat
      181 tcgccgccgc gcctctcgag caaccgtatg gtgccggagg catagccgcc gttccacagc
      241 tcgttatgct tcttctcgcc gtcggggttc tcgtagttga cattgagcat ggtatcccgg
      301 gggcacgcga aacggatctc gaccttggcc gtgccgtgcc acgcgtcgat gttccactcc
      361 accctgtcgt cgcgcaccgg gcagtcgaag acctgcttgg agccggtcca gaacttggag
      421 aaattccact cgtagaaagc cccctcgtag tgcagggcga tgagcagccg cctgggcagg
      481 gagatgccga aagccactgg ctgcgcgccg ccgacgtcga ggctcgatcg ctccaggagc
      541 ttcccggtct tgcggctcct gaggttgttg cagttcagcc agacccaggg gctcgtatag
      601 tcgcggcccc agttcttgtc ctggtagccg aacgagtcgt cgggcgagac gatgaagcgc
      661 tcgccgttga aggtgatgac gcccttgtag cgggtgcgca tgcccgccac gtgccagtac
      721 atattgaagg cgtccagctt gcggaaaaag gcgctcgtgc cccagccgac gctgaagcgc
      781 aggattttct cggcctccag gtcccagctc atctcgcccg agtcggacat gtactccggg
      841 tgggccgcgg cgtcggcggc gctcagcgag acggagccct tgagcctggt ctcggtggcg
      901 acgtgatcgc cgatgcgcac gtccatgccg tcccgcgagg cggagaacgc cgccacgggg
      961 tacaggttat ggagctgggc cttggctgcg gcgccccagc agccagcctt gatcatcgcg
     1021 tagcacggct tccttccggc cttcttgttg gccggaagct ggccagccac cggctcggcg
     1081 tcaccgaacc cggggttgat cacgtagtac tcgatgaaga acggcctgcg ctcaccggtc
     1141 ttctcgctca tagccacgag agaatgccac caccagtcgt acccgcgccg cgcccgcgcg
     1201 ccagagagca tgtaaccgtt acgctcgtcg cccatcggtg cctcctagcg gggcggctcg
     1261 tgtccggccc gcggctacga tgatagccca taaatttgca ctatgcaaat ttaatgtaca
     1321 gcgtatacta taatcatggt ccgggctggt cccgaccggg aggctacaga tggcgactgt
     1381 cacgatcgag cgattgccgg ccttatccgt cggcgcttcg gtgccgctgc cgaacggcta
     1441 cgtcgactcg ggctacgcgg aggactgcgc cagcctgccc cgcgactatg gcacggagtc
     1501 ctgggaggca cggccggaag tgatcgagat acgccgggcg gtattcgcgg cgctcgaccg
     1561 cctcgaaggg gagacgtcca tagcctcgcg cttccgggga cggcccgtcc tcgtcaagcc
     1621 gaacctcgtg accgtctacc aggatatggg aatgcgacgc gggtcctacc cggagaccac
     1681 ggacccgcgg gtgctcgacg cggtggtgct atggctcgga cgcttcgcgt cgacgatacg
     1741 catcgtcgaa tcgtcggggc gcggggcccc gacccgcggc tccttcaggg tctcgggtat
     1801 agatcggctg gccaggcacc gcggctgcga cctggtcgcg ctcgaggaga cgccggtaga
     1861 ccgctacctg ctacccaagg cctccgcgca acgggagata ctcgtgcccg aggccttctc
     1921 cgccgtcgta cgcggggagg cggcctacgt ctcggtgcct aagctcaaga cgaacctgta
     1981 tactggcgtg acgctcggct tcaagaacgc gatgggcgtc ataccctata acctgcgcca
     2041 gcgcaaccac cactacgcca tcaaccgcaa gctcgtcgag atgctatacc tgttcaagcc
     2101 ggacctggtc gtgatcgacg gcgtcgtcgg cggcgagggc gagtgcccgg caccggtcga
     2161 tccggtggac tcgcgcgtga tcatcgcggg cgaccacccg gtggagaccg accgggtcgc
     2221 gacgcggctg atgggcttcg accccggatc gatcgagctg atgcgcgtcg ccgacgaact
     2281 gggcttcggg gacgccacgg gcgcccgggt cctggccaat ggtttcgacg cgccggcccg
     2341 cgaccaaggc ggcctatcgt cggcgcggag cgccatcccc gacctgggcg gcgccgtccc
     2401 agtacgattc tgcccggccg actggtccct gacctcggag cgcgtccgcg gggccttccc
     2461 cgggatactc gtgctgatag gcatcgaccg cggcggactc gagggcaagt ccggcccgga
     2521 actcgtcgag gcgatgcagg cctcgtgccg aggcggctgc gtggcgacca cccgcttcgc
     2581 cttctcgatg ttcgaggcgg agggcgtacg gccgaggagg cccgtggcgc tgatcatagg
     2641 ctgcggcctg gagcgcgacg gccgccggct ctggttcgac gccgacggca agccctacgc
     2701 cgaggacgac atccatgcgc tgcgcatgcg caaggccgtg atcggctcgt gcacccgcgt
     2761 cctggcgccg ctggcgacgc gcttcgtcga cggctgcatg gtgctgccga acgcgccgca
     2821 cgtggtgctc cacgagctgt ccgggacccc gtgcagggtc atgtcgctgc gcaacaggaa
     2881 cctgttcccg atgctcgccg cgaccctcgg gcagcgcgcg gcccggaggc gcctgatcga
     2941 gtcgggcaag cgcctcgacg tgccgctcag gctgaacgac gagccgatca agaccaggga
     3001 gctggccccg ggcgaggaga agcgcgacag ggtcgcctgg cccatgccgc cgatcgagcc
     3061 cgaggagatg tgtcggctcc tagacttcga ggacgacgcg acgctggcct cgctacgcgg
     3121 tatcatcgtc ttcaggatca aggaacgcgc gctctggcgc gccaagcagg tcctggccac
     3181 gatcgtgacg ctcgggccgc tcgtcctcgg ggcactggcc gccatcgaag tttccaggtt
     3241 cgggctcggc acggcggcct ggctggccat cttcggtgcg gtggaggcga tacacctggc
     3301 cgagctgccc gccgcgctca gagcctggaa gaaagaggcc gagcgccgca acgcgccgtg
     3361 gtcgggggca aggaccgttc gcgccgtggc cgccaccctg agctgcggct acccgagctg
     3421 ggtaccgagg aagctgggcg tcttcgatta acccggagcc gcgcggcttg accttcagag
     3481 cgctttacta gaagcttcct tcggagccgc cgatccggcc ccgaaggaac cgctcatacc
     3541 atgatatctc cgatccagca caacggcatc ttcctcggtc tctcctcgtt ccaggctctg
     3601 gccatgttca ggcggggcat cttctacacc ttcctagcgg tctacctgcg ctcccacctg
     3661 ggtctgtcgg ttaccgagac cacgctgttc gagaccgtac cgatggcgct caacgtgctg
     3721 ttccagaccc tcgtctgggg tcgcctgacc gaccggcttc agaaacgtcg gaccttgatc
     3781 atcatcggcg agctcatggc cagcgtcggc catgtggcca tgtggtactt ccacgccgtc
     3841 gcgcctaccc cgcgcgcggc cggctacgtg atcatagcgg gactgacggt gatcgaggcc
//
LOCUS       DAOOLN010000031         3833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_28797_length_3833_cov_2.868184, whole genome shotgun sequence.
ACCESSION   DAOOLN010000031 DAOOLN010000000
VERSION     DAOOLN010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28797_length_3833_cov_2.868184"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PLW80_00715"
     CDS             <1..635
                     /locus_tag="PLW80_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPB65051.1"
                     /translation="RLADGSTGSVTVGGTVSPAGGNFEEPVTQATLKVVGAFHGLSRE
                     RSDARKYPAIHPLDSWSKYGGLLDASSVAWAHSFLKRGTEVGQMMKVVGEEGTSLDDF
                     LVYLKGEFLDDVYLQQNSFDAVDASVSPERQKASFRSCVRILGSSFDFKDKDAARAWF
                     NQLRQRFLDGNGIEWGTDAYVSAEAELKAFIDERVSGLDKKAARIIESLE"
     gene            641..1937
                     /locus_tag="PLW80_00720"
                     /pseudo
     CDS             641..1937
                     /locus_tag="PLW80_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968955.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
     gene            1941..2555
                     /locus_tag="PLW80_00725"
     CDS             1941..2555
                     /locus_tag="PLW80_00725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPB65052.1"
                     /translation="MAKIKLTKNELKKQKDALKMYKRYLPTLQLKKQQLQTEIRGIEA
                     RARDIAAERDRHLAEFQAWVAVFGEEDAIKRPDGGWIISSGTIRTSVGNIAGVDIPVF
                     KDADFAIDEYDLFAKPLWIDRALERLRAMLTLDLEIKVLEEQIARLSRELRTTTQRVN
                     LFEKVKIPETAASIKKIGIYLGDQQTAQVVRGKIAKRKVERIAS"
     gene            2552..>3833
                     /locus_tag="PLW80_00730"
     CDS             2552..>3833
                     /locus_tag="PLW80_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HPB65053.1"
                     /translation="MIAPMKRAFVMMMEREKKEALHALRRLGSLHVEPLEGVGGDYDE
                     LSASVAAMREAIGLLSEFKAEKQLEELNMRDGLDLANRVRRLDAESKAANDEAAATAR
                     EIERVRPWGDFDPSAIAELSGKGIPLRLVELSAKKLEAASFGPDFIRLGEAKGSVRLA
                     VIAGPEAALPSDCVEFRLPAKGLSELEAELIDARSRASAARQAMAAEAPRSEGLKKAL
                     ARVERDMAFEALRSGMSAGDGVSWLAAWVPAKEEKRLSDLAAKEGWGLLLDDPLEEEQ
                     PPTKIENPAIIRMIQPVFDFLGTVPNYREYDISGLFLLFFSVFFAMIFGDGGYGGILL
                     LAGAALAIKSKASGKPVPDFIRLLVVLAAKTVVWGALTMSWFGLPPERVPTLLKSLSV
                     YWVSNANPDSGNNVKILCFMLGLVHLSIAHLMNIR"
BASE COUNT          722 a         1252 c         1238 g          621 t
ORIGIN      
        1 tccgcctcgc ggacggctcc accggctcgg tcacggtcgg cggcacggtc agcccggcgg
       61 gcggcaactt cgaggagccg gtcacccagg ccacgctcaa ggtggtcggc gccttccacg
      121 gcttgtcccg cgagcgctcc gacgcccgca agtaccccgc gatccacccg ctcgactcct
      181 ggtccaagta cggaggcctg ctcgacgcgt catcggtagc ctgggcgcat tccttcctta
      241 agcgcggcac cgaggtcggc cagatgatga aggtcgtcgg cgaggaaggc acgagcctcg
      301 acgacttcct cgtctatctc aagggcgagt tcctcgacga cgtgtacctg cagcagaact
      361 cgttcgacgc ggtcgacgca tccgtgtcgc cggagcggca gaaggcgtcg ttccggtcct
      421 gcgttcgcat cctcggctcg tcgttcgact tcaaggacaa ggacgccgcc cgggcctggt
      481 tcaaccagct caggcagcgc ttcctcgacg gcaacggcat cgagtggggt accgacgcgt
      541 acgtgtcggc cgaggccgag ctcaaggcct tcatcgacga gcgggtatcg gggctcgata
      601 agaaggcggc ccggatcatt gagagcctgg agtaacggat atgaagaagg tatattccca
      661 cattgaatcg atcaagggca gcgtcatcac cgtgcgggcc accggcgtga cctacgggga
      721 gctggccgag gtcaccacgc gcttcggcgt ttcgctcgcc gaggtcaata ggctcgacga
      781 ggatatggtc tcgctccagg tcttcgccgg aggccgaggc atctcgaccg gcgacgaggt
      841 gcgtttcctg ggcaacccga tgcgcgtcag cttcggcgac tcgctcaagg gccgtatctt
      901 caccggctcg ggcgagccgc gcgataacgg cccgacgctg agcgagaacc tgatcccgat
      961 aggcggcccg tcggtcaatc cgacgaaccg catcatcccg cgcgagatgg tgcgcaccgg
     1021 catcccgatg atcgacctct tcaacacgct cgtgaagagc cagaagctgc cgatcttctc
     1081 gatctccggc gagccgtaca accagctcct cgcgcgcatc gcgatgcagg ccgaggtcga
     1141 cctgatcatc cggcggcatg ggcctgaagt acgacgacta cctgttcttc aaggacacgc
     1201 tggaggaggg cggctcgctc cagaagacga tcatgttcgt ccataccgcg gccgacccgg
     1261 tcgtcgagtg cctgatggtg ccggatatct ccctggccgt cgcggagcgc ttcgccctca
     1321 agggcctcaa cgtgctcgtg ctgctgacgg acatgaccaa cttcgccgac gcgatgaagg
     1381 agatagcgat cacgcaggag caggtgccgt cgaaccgcgg ctatcccggc gacctgtact
     1441 cgcagctggc ctcccgctac gagaaggctg tcgacttcgc cgactcaggc tcgatcacga
     1501 tcctcgcggt gacgacgatg cccggcgacg acgtgaccca cccggtgccc gacaacaccg
     1561 gctacatcac cgagggccag ttctacctga agggcggccg catcgagccg ttcggctccc
     1621 tatcgcgcct caagcagcag gtcaacggcc gcaccagggc cgaccatcgc gccatcatgg
     1681 acggcatgat caagctgttc tccgcctacc gcgacactct cgagaagaag tcgatgggct
     1741 tccagatgtc ggcctgggac cagaagctgc tcaagtacgg cgagctgttc gagagcgaga
     1801 tgatggacct gtcggtcaac atcccgctcg agggcgcgct cgacaagggc tgggagatac
     1861 tggcggcctg cttcgagccc caggagaccg gcctccaatc caagctgata gaccagttct
     1921 ggcccaagaa ggcatgagtc gtggcaaaga tcaagctgac taagaacgag ctcaagaagc
     1981 agaaggacgc gctcaaaatg tacaagcgct acctcccgac gctccagctc aagaaacagc
     2041 agctgcaaac cgagatccgc gggatcgagg ccagggcgcg ggacatcgcc gccgagcgcg
     2101 accggcacct ggccgaattc caggcctggg tagcggtctt cggcgaggag gacgcgatca
     2161 agcgccccga cggcggctgg atcatctcct cggggacgat acgcacgagc gtcggcaaca
     2221 tcgcaggcgt cgacatcccc gtgttcaagg acgccgattt cgcgatcgac gagtatgacc
     2281 tgttcgccaa gccgctctgg atagaccggg cgctcgagcg cctgcgggcc atgctgacgc
     2341 tcgacctcga gatcaaggtg ctcgaggagc agatagcccg gttatcccgg gaactgcgca
     2401 cgacgacgca acgcgtcaac ctgttcgaga aggtcaagat cccggagacc gccgcgagca
     2461 tcaagaagat cggcatctac ctcggcgatc agcagaccgc ccaggtcgtc cgcggcaaga
     2521 tagccaagcg caaggtcgag aggatagcgt catgatagcc ccgatgaaac gcgccttcgt
     2581 catgatgatg gagcgcgaga agaaagaggc gctccacgcg ctacgcaggc tcggtagcct
     2641 ccacgtcgag cccctcgagg gcgtaggcgg ggattatgac gagttgtcgg cctcggttgc
     2701 ggctatgcgc gaggcgatcg ggctcctgtc ggaattcaag gccgagaagc agctcgagga
     2761 gctgaacatg cgcgacggtc tcgacctggc caatcgagtg cgccgcctcg atgccgagtc
     2821 caaggcggcg aacgacgagg ccgcggcgac ggcccgcgag atagagcgcg tcaggccatg
     2881 gggagacttc gacccgtccg cgatcgcgga actatccggc aaggggatac cgctccgcct
     2941 cgtcgagctg tcggccaaga agctggaggc cgcgtcgttc gggccggact tcatccgcct
     3001 cggcgaggcg aaggggtcgg tcaggctggc cgtgatcgcc ggacccgaag cggctttgcc
     3061 gagcgactgc gtcgagttcc gcctgcccgc caaggggctt tcagagctgg aggccgagct
     3121 gatcgacgcc cgctcgaggg cctcggcggc ccgccaggcg atggccgccg aggcgccgcg
     3181 ctccgagggt ctcaagaagg cgctggccag ggtcgagcgc gacatggcct tcgaggccct
     3241 gcgctccggc atgtccgccg gcgacggcgt atcctggctg gcggcgtggg tgcctgccaa
     3301 ggaggagaag cgcctgtcgg acctcgccgc caaggaaggc tgggggctac tcctcgacga
     3361 cccgctcgaa gaggagcagc cgcctaccaa gatagagaat ccggctatca tcaggatgat
     3421 acagccggta ttcgacttcc tcggcaccgt gcccaactac cgcgagtacg acatctccgg
     3481 cctgttcctc ctgttcttct cggttttctt cgcgatgatc ttcggcgacg gcggatacgg
     3541 cggcattctg ctactggccg gtgccgcgct ggcgatcaag tcgaaggcct ccggcaagcc
     3601 ggtgcccgac ttcatcaggc tcctcgtggt gctcgcggcg aagaccgtcg tctggggagc
     3661 gctgacgatg agctggttcg gcctgccgcc cgagcgcgtg ccgacccttc tcaagtcgct
     3721 gtcggtgtac tgggtatcga acgccaatcc cgattcgggt aacaacgtca agatactctg
     3781 cttcatgctc ggcctggtgc acctgtccat cgcccacctg atgaacatcc gcc
//
LOCUS       DAOOLN010000032         3826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_28881_length_3826_cov_2.739592, whole genome shotgun sequence.
ACCESSION   DAOOLN010000032 DAOOLN010000000
VERSION     DAOOLN010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28881_length_3826_cov_2.739592"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..468
                     /locus_tag="PLW80_00735"
     CDS             40..468
                     /locus_tag="PLW80_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HPB65054.1"
                     /translation="MITGQLAKQLGAKKGVAVILSDRYMPLGAKLDVDALVSIKGVVA
                     AAVLETVRRANIRTIHGFYEDDVELVELTVGAASTVAGRSLREIDLPKGVLVAFVIHG
                     EAMLVPTGATTLNVGDVLGLVSRKKSIPGLERVFGGDRGA"
     gene            458..1915
                     /locus_tag="PLW80_00740"
     CDS             458..1915
                     /locus_tag="PLW80_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPB65055.1"
                     /translation="MELKPLAKALAVLLAIVALFMAGAMVAGLLLGEGSAALGAFAPS
                     VAIGLGALVATQALIRFRSGLSLSHRSGYLFVTLAWVLSAALGALPFRLYGAVPSYTD
                     AFFETMSGFSTTGASILADIESLPESLLLWRAMTHWLGGMGIVVLTVAIMPLLGLSAR
                     SVMEAEAPGPQVDKFTPRLSQTAKILWLIYLGLTAALTVLLLFGGMSLIDALTHAFAT
                     MATGGFSTRNASVGAFGSTYVDVVLTVFMVLAGVNFSLHWSALRGDLRKVLADTELRV
                     YLAVFAAASVVIVLDLRRAGLFDSVGEAARYASFQASSILTTTGFATADYTLWPPLSQ
                     AVLFTLMFIGGCAGSTGGGVKVSRIVTLFKMGQSEMRYLLNPRGVYGVFLDGRHLKKN
                     VVYDIAAMVFLYLVAAFVSTMAVAAGGYDILTSLSATMACLGNIGPGFALVGPACNYG
                     FMPGALKWWLSFAMLLGRLEVYTVLVLFTRAFWRR"
     gene            complement(1924..3192)
                     /locus_tag="PLW80_00745"
     CDS             complement(1924..3192)
                     /locus_tag="PLW80_00745"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB65056.1"
                     /translation="MLFLLAGLSQTGRLRQYERSAVLLGASTVRVAIEGSLNEHISLE
                     RGIVAFVASDTGLDSEVYDRYARALAMDDPIIINLAVLEGTVIKFVYPYDQNTNAIGK
                     DLATVPDQAAAIVRAMTTRDPIVSGPHELVQGGYGVISRMGIFPIGPSGPEYWGQASV
                     VVDVNEILRRSGVAEHDALSFLLCAGAEGATDAATIFGDRAVLGQDPVVLDIRLPGAT
                     WTLAAVPKGGWKSFGWLTFGGAWIGAVIGLLAGFALFSLITTRGALKELAYHDQLTNL
                     PNRTLFWDRLHVEAARAERDGQRVCVCMVDLDGFKAVNDDLGHESGDRLLKQAAARMS
                     EALRRSDTVARLGGDEFAVIAQVDSPSGVDEVTARIKGCFAEPFDLGSVKRASSASIG
                     GALYPDDGTDVEAVLAKADHRMYARKHAAR"
     gene            3614..>3826
                     /locus_tag="PLW80_00750"
     CDS             3614..>3826
                     /locus_tag="PLW80_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB65057.1"
                     /translation="MPAIIARDLSKTFKTKVRPSGRLAALRSLVKPEYRDVRAVKGVS
                     FEIDDGELVAFLGPNGAGKSTTIKMLM"
BASE COUNT          574 a         1293 c         1271 g          688 t
ORIGIN      
        1 tacctcgtcg tctgcgccac ggaatctcaa tagttcaacg tgatcaccgg ccagctcgcc
       61 aagcagctcg gcgccaagaa gggcgtcgcc gtcatcctga gcgaccgcta catgccgctc
      121 ggcgccaagc tcgacgtcga cgccttggtc agcatcaagg gcgtcgtggc cgccgccgtg
      181 ctcgagacgg tacgccgagc gaacatcagg acgatccacg gcttctacga ggacgacgtc
      241 gagctcgtcg agcttaccgt cggggccgcc tcgacggtcg ccgggcggtc gctcagggag
      301 atcgacctgc ccaagggcgt gctcgtggcc ttcgtgatcc acggcgaggc catgctggtg
      361 ccgaccgggg ccactaccct caacgtcggc gacgtgctcg gcctcgtgtc gcgcaagaag
      421 agcattcccg ggctcgagcg ggtcttcggt ggcgaccgtg gagcttaagc cgctggccaa
      481 ggccctggcg gtgctgctgg ccatcgtcgc gcttttcatg gcaggggcga tggtcgccgg
      541 cctcctcctg ggcgagggct ccgccgcgct cggggccttc gcgccgtcgg tcgccatcgg
      601 cctcggggct ctcgtcgcca cgcaggcgct gatacggttc aggagcggct tgtcgctatc
      661 ccatcgctcc ggctacctgt tcgtcaccct cgcctgggtc ctgtcggccg cgctcggcgc
      721 cttgccgttc aggctctacg gcgccgtgcc gtcgtatacg gacgcgttct tcgagaccat
      781 gtccggcttc tcgacgaccg gcgcctccat cctggcggac atcgagtcgc tgccggagtc
      841 gctgctcctg tggcgcgcca tgacccactg gcttggcggc atgggcatcg tggtgctgac
      901 ggtggccatc atgccgctgc tcggcctgag cgccaggtcg gtcatggagg ccgaggcgcc
      961 gggcccccag gtggacaaat ttacgccgag gctgtcccag accgccaaga tactctggct
     1021 gatctacctg ggcctgacgg cggcgctgac cgtactcttg ctgttcggcg gcatgtctct
     1081 gatcgacgcg ctgacccacg ccttcgcgac gatggctacc ggcggtttct cgacgcgcaa
     1141 cgccagcgtc ggagccttcg gctcgaccta cgtcgacgtc gtgctgacgg tcttcatggt
     1201 gctcgccggc gtcaacttct cgctgcactg gagcgcgctg cgcggcgatc tcaggaaggt
     1261 gctcgccgac accgagctca gggtgtacct ggccgtcttc gccgcggcgt cggtcgtcat
     1321 cgtcctcgac ctgcgccggg ccggcttgtt cgactcggta ggcgaggcgg ccaggtacgc
     1381 gtcgttccag gcctcgtcca tattgacgac gacgggcttc gcgacggccg actatacgct
     1441 ctggccgccc ctatcccagg ccgtgctgtt cacgctgatg ttcataggcg gctgcgccgg
     1501 gtcgaccggc ggcggcgtca aggtctcgag gatagtcacg ctgttcaaga tgggccagtc
     1561 ggagatgcgc tacctgctca acccgcgcgg cgtctacggc gtgttcctgg acggtaggca
     1621 cctcaagaag aacgtggtct acgacatcgc cgccatggtg ttcctgtatt tggtagcggc
     1681 cttcgtctcg acgatggcgg tagccgccgg cggctacgat atcctgacca gcttgagcgc
     1741 gacgatggcc tgcctcggca acatcggccc gggcttcgcc ctcgtcggcc cggcctgcaa
     1801 ctacggtttc atgcccggcg cgctcaagtg gtggctgtcc ttcgcgatgc tgctcgggcg
     1861 gctcgaggtc tacacggtgc tcgtgctatt cactcgggcc ttctggcggc gctgaggctc
     1921 gcctcagcgc gccgcgtgct tcctggcgta catgcggtgg tcggctttcg cgaggacagc
     1981 ctcgacatcg gtgccgtcgt cgggatagag cgcgccgccg atgctcgccg acgaggcccg
     2041 cttgaccgag ccgaggtcga acggctcggc gaagcaaccc ttgatccggg ccgtcacctc
     2101 gtcgacgccg gacggggagt ctacttgggc tatcaccgcg aattcgtcgc cgccaagccg
     2161 cgccaccgtg tcgctcctgc gcagggcctc gctcatcctc gccgccgcct gtttcaggag
     2221 ccggtcgccc gactcgtgcc ccaggtcgtc gttgaccgcc ttgaagccgt cgaggtcgac
     2281 catgcagacg cagacccgct ggccgtcccg ctcggcccgc gccgcctcga cgtggagcct
     2341 gtcccagaac agggtccgat tcggcaggtt ggtcagctgg tcgtggtagg ccagctcctt
     2401 gagggcgccg cgggtcgtga tcaggctaaa gagcgcgaag ccggcgagga gcccgatcac
     2461 ggcccctatc cacgcaccgc cgaaggtgag ccagccgaag ctcttccagc cgcctttcgg
     2521 cacggcggcg agggtccagg tggcgcccgg caggcgaata tcgagaacca ccggatcctg
     2581 cccgagcacg gcccggtcgc cgaagatcgt ggcggcgtcc gtggccccct cggcgcccgc
     2641 gcagagcagg aaggacagag catcgtgctc cgcgacgccg gagcgcctga gtatctcgtt
     2701 gacgtcgacg acgacgctgg cctggcccca gtactcgggg ccgctcggcc cgatcgggaa
     2761 gatccccatg cggctgatga cgccgtagcc gccctgcacg agctcgtggg ggccggagac
     2821 gatcgggtcc ctcgtcgtca tggcgcggac tattgccgcg gcctggtccg gaacggtcgc
     2881 caaatccttg cctatcgcgt ttgtgttttg atcgtacgga tatacgaatt tgatcacggt
     2941 gccctcgagc accgccaggt tgatgatgat ggggtcgtcc atggcgaggg ctcgggcgta
     3001 gcggtcgtac acctccgaat ccaatcccgt gtccgacgcg acgaaggcga cgatgccgcg
     3061 ctcgaggctg atatgctcgt tcaggctacc ctcgatggcc acgcggacgg tcgaggcgcc
     3121 gagcaggacg gcgctgcgtt cgtattgcct gagccttccg gtctgcgaca gcccggccaa
     3181 caagaagagc atgaaaatgc cgacgaacgc ggcggctccc gcgaggacgg ctctcgtcgg
     3241 cttacgagat gctaatggtc ccagctgagt actcgatcga tgcatggctc aagcgtatag
     3301 tggccgggcg ctcgttgcaa gccacgatca cgtcgtggta gctctccata tagaaacact
     3361 tcgctcgatt gactgaaccg acccgcgggg ggtaaagatt acgtagcact ttcgagaggg
     3421 cgcctggggg ttcctcccgt tccgagcggg acggggatga gaccgagcgg cgccggcggc
     3481 cgcgtgacgg ccgcgacacc cacgggataa aagcccgagg acggagtctc gttagattac
     3541 ggggtccgtc cgcccgccct accgtagcgt atggctaaag cgtcgaacgg ccccgctcta
     3601 gcggaggctc cccatgcccg cgatcatcgc gcgcgatcta tccaagacct ttaagacgaa
     3661 ggttcgcccg tcggggcgac tcgcggctct caggtcgctc gtcaagcccg agtaccgcga
     3721 cgtccgagcc gtgaagggcg tgtccttcga gatcgacgac ggcgagctcg tcgccttcct
     3781 ggggccgaac ggcgcgggca agtccacgac catcaagatg ctcatg
//
LOCUS       DAOOLN010000033         3779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_29361_length_3779_cov_2.919173, whole genome shotgun sequence.
ACCESSION   DAOOLN010000033 DAOOLN010000000
VERSION     DAOOLN010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29361_length_3779_cov_2.919173"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLW80_00755"
     CDS             <1..474
                     /locus_tag="PLW80_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65058.1"
                     /translation="REEIDTAIRARKVEGKHVIPVPAIEIKRSYPRIFYDSIRVFLQR
                     NLSPGGLTTPKLYEKSVVRPEFSKRGRVAISESALSQMVIHCIDEFDDAIRIQKITVR
                     SDSQGYRLTVVIEVPFGSQLSGKIHNLQQYIIDNIERFTGILIEEVNLVIDRFSK"
     gene            528..1826
                     /locus_tag="PLW80_00760"
     CDS             528..1826
                     /locus_tag="PLW80_00760"
                     /inference="COORDINATES: protein motif:HMM:NF019239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB65059.1"
                     /translation="MEGTIVKKILSFAVIALALAVCAGAQDAAGVTNPAPAAAPVAAP
                     EATTEVVAVPAAQPTAFSSASSKRYAVWSDAGKDDAVRLATTLDGLFAVYNEYFRFDE
                     SALKAPLTVRKFAEKASFDAYLAKVIGETKDDFVYLHYPTIERSELVIFGKASADDFD
                     ASLAHQAFVQFLKAFVPEPPLWIREGFAVFFERTRANPSTGVVAYAENQSWLETIKLL
                     KSQDRLYTVSELMLLSSDEAKSGLDVFYPQSWALVSFFINSPDRRYNRFVWDAIGLLA
                     RDATLEQNQNAVRDFFLRWHGQEAAESDFLTYLDGQKTFAELVTSGVRSYGDKDLETA
                     YKAFSQALTKNAASYIPHYYLGLIAYARGDYALAQDYYLKALELGSDAPITNYALGIN
                     AYASRRFDEARAYLQKASDGDPTKYQEKAGEIITRISSGM"
     gene            complement(1924..2721)
                     /locus_tag="PLW80_00765"
     CDS             complement(1924..2721)
                     /locus_tag="PLW80_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB65060.1"
                     /translation="MRIRFLGTGTSHGVPVLGCSCPVCSSTDPRDTRYRSSALVTGDG
                     GERILLDAGPEFRLQALAARLDALDALLVTHAHADHVHGIDDVRPLTRGRPLPVYASG
                     LDASELRHRFSYVFSAGQAGGGKPRLELLDLDGPDGTGSAPRQVSVGSLVVRPVPLSH
                     GGRRVLGYRLDGLAYATDCSSVPDSSKAMLRGLDLLVLGALRLRPHPTHLSVDEALAL
                     ARELRPARLLLTHICHDLSHERLQSYCDDAGLPFPAGPAYDGLELEV"
     gene            complement(2718..>3779)
                     /locus_tag="PLW80_00770"
     CDS             complement(2718..>3779)
                     /locus_tag="PLW80_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65061.1"
                     /translation="GPLYAQGSWIIKRSAADGSVLQAKVFVRSDPGTFVRLYPYGSRS
                     RIDVVAYGGVLYHKAIIPLPFEELLRSPFSRIVGLTSDVIDWSRFSPDPALYRDLRGL
                     MASVRAGLPSLRYADDGAIDADGRAVLISTLADQAKPAGLNCSGFVKWLVDGMLHPIT
                     GNYLAVGRLKERMLDRRGSSFTVGFEERYDPFFGLDWSRALARAAWSAFYPSLRDESP
                     LANDVAEAPFALRVSDADPVNGGSAYEPYSDNFDDAGLDVRGLKAVLFMLASSEPGRF
                     YLAQFNARESAPPNLRRYFHIAALFPYFDEDGAFRVAVFESAAETSLDAVTRRGYEFV
                     KLVRMPAASRFEPQALDRP"
BASE COUNT          666 a         1255 c         1279 g          579 t
ORIGIN      
        1 cgcgaggaga tcgacaccgc gatccgcgcc cgcaaggtcg agggcaagca cgtcatcccc
       61 gtgcctgcca tcgagatcaa gcgcagctac ccccgtatct tctacgacag catacgggtg
      121 ttcctgcagc gcaacctgtc gcccgggggc ctcacgacgc cgaagctgta cgagaagtcg
      181 gtggtgcgcc ccgaattctc gaagcgaggc cgcgtcgcca tttccgagtc ggcgctgtcg
      241 cagatggtca tccactgcat cgacgaattc gacgatgcga tccgtatcca gaagatcacc
      301 gtccggtcgg actcgcaagg ctacaggctg accgtcgtga tagaggtacc gttcggctct
      361 cagctgtccg gcaagatcca caatctgcag cagtacatca tcgacaacat cgagcgcttc
      421 accggtatcc tgatcgagga ggtgaacctg gtcatcgacc ggttctccaa gtaggatgtt
      481 cggtcccgtt tgacaacgga ctggacgggg tttaggataa acgtaccatg gagggtacca
      541 tagtgaagaa aatactgagc ttcgcggtca tcgccctggc gctggccgtg tgcgccggcg
      601 cgcaggatgc cgcgggcgtg accaatcccg ccccggcggc ggcgccggta gccgctcccg
      661 aggctactac cgaggtcgtg gccgttccgg ccgcgcagcc gaccgctttc agcagcgcca
      721 gctccaagcg ctacgccgtc tggtccgacg ccggcaagga tgacgccgtc aggctcgcga
      781 cgaccctcga cgggctcttc gccgtgtaca acgagtactt ccgcttcgac gaatcggccc
      841 tcaaggcccc gttgacggtc aggaagttcg ccgagaaggc ctcgttcgac gcctacctgg
      901 ccaaggtgat aggcgagact aaggacgact tcgtctacct gcactacccg acgatcgagc
      961 gctccgagct ggtcatcttc ggcaaggcca gcgccgacga cttcgacgcc tcgctcgccc
     1021 accaggcctt cgtgcagttc ctcaaggcct tcgtcccgga gcctccgctc tggatccggg
     1081 aaggcttcgc tgtattcttc gagcgtaccc gggccaatcc gtccaccggc gtcgtagcct
     1141 acgccgagaa ccagtcctgg ctcgagacca tcaagctgct caagtcgcag gatcggctct
     1201 acaccgtctc cgagctgatg ttgctgtcct ccgacgaggc caagagcggg ctcgacgtgt
     1261 tctacccgca gtcctgggcc ctcgtctcgt tcttcatcaa tagccccgac cggcgctata
     1321 accgtttcgt ctgggacgcc atcggcctcc tggcccgcga cgcgacgctc gagcagaacc
     1381 agaacgccgt cagggacttc ttcctgcgct ggcacgggca ggaagcggcg gagtcggact
     1441 tcctgaccta cctggacggc cagaagacct tcgctgagct ggtgacctcc ggggttcgct
     1501 cctacggcga caaggacctc gagaccgcct acaaggcctt ctcccaagcc ctgaccaaga
     1561 acgccgccag ctacatcccg cactactacc tgggcctgat cgcctacgcc cgcggcgact
     1621 acgcgctggc gcaggactac tatctcaagg ccctcgagct cggtagcgac gcgcccatca
     1681 ccaactacgc gctcggcatc aatgcctacg cctccaggcg cttcgacgag gcccgggcct
     1741 acctgcagaa ggcctcggac ggcgatccga ccaagtacca ggagaaggcc ggcgagatca
     1801 tcacccgcat ctccagcggc atgtgatacg aacgagccta tcgtagcgag ttaccaaggc
     1861 cgccccgaaa ggggcggctt ttttgtggcg aacgcttgta tatggggctt ttccggccgg
     1921 cgcttagacc tcgagctcga gcccgtcgta ggccgggccg gccgggaagg gcaggccggc
     1981 gtcgtcgcag tagctctgga gccgctcatg ggacaggtcg tggcagatat gggtcaggag
     2041 cagcctggcc ggccgcagct cgcgggccag cgccagcgcc tcgtccaccg acaggtgcgt
     2101 cgggtgcggc ctgagcctca gcgcgccgag cacgagcagg tcgaggccgc gtagcatagc
     2161 cttcgacgag tccgggacgg acgagcagtc ggtcgcgtag gccaggccgt cgagcctata
     2221 gccgaggacg cgccgcccgc cgtgcgagag cggaacgggg cggacgacga gcgagccgac
     2281 gctgacctgc cgcggcgcgg aacccgttcc gtccggcccg tcgaggtcga ggagctcgag
     2341 ccgcggcttg ccgcctccgg cctggccggc cgagaacacg tacgaaaagc ggtgccgcag
     2401 ctcggacgcg tccagtccgg aggcgtagac cggtagcggc cggccccgcg tcagcgggcg
     2461 cacgtcgtct ataccatgca catggtcggc gtgggcgtgg gtgacgagga gcgcgtcgag
     2521 ggcgtcgagg cgggcggcga gcgcctgtaa ccggaattct gggcccgcgt cgagcaggat
     2581 gcgctcgccg ccgtcgccgg tcaccagggc ggaggagcgg tagcgcgtgt cgcgcgggtc
     2641 ggtggacgag catacggggc aggagcagcc gagcaccggc acgccgtggc tcgtgccggt
     2701 gccgaggaag cgtatcctca cggtcggtcc agggcctggg gttcgaagcg gctagccgcc
     2761 ggcatgcgca cgagcttgac gaactcgtag ccgcgccgcg tcaccgcgtc gagggacgtc
     2821 tccgccgcgc tctcgaagac cgccaccctg aaggcgccgt cctcgtcgaa gtacgggaag
     2881 agggcggcga tatggaaata gcgccgcagg ttgggtgggg cgctctcgcg ggcgttgaac
     2941 tgggcgaggt agaagcggcc gggctcggag gatgccagca tgaacagcac ggccttgagg
     3001 ccgcgcacgt cgaggccggc gtcgtcgaag ttgtccgaat acggctcgta ggccgagccg
     3061 ccgttgaccg gatcggcgtc ggagacgcgc agcgcgaacg gcgcctcggc gacgtcgttg
     3121 gcgagcggcg actcgtcgcg cagcgacggg tagaaggcgg accaggccgc ccgggccaac
     3181 gcgcgcgacc agtcgagccc gaagaacggg tcgtatcgct cctcgaagcc gacggtgaac
     3241 gaggagccgc ggcggtcgag catgcgttcc ttgagcctgc ccaccgccag gtagttcccc
     3301 gtaataggat gcaacatccc gtcgaccagc catttcacga agcccgagca gttgaggccg
     3361 gcgggtttcg cctggtcggc gagcgtcgag atgaggacgg cccgcccgtc ggcgtctatc
     3421 gctccgtcgt cggcgtagcg cagcgacggt agtccggcgc gaaccgaggc catgaggccg
     3481 cgcaggtccc gatacagcgc cgggtcgggc gagaatctgc tccagtcgat cacgtcggag
     3541 gtcaggccga cgatccgcga gaacggcgag cgcaacagct cctcgaaggg gagcgggatg
     3601 atcgccttgt ggtagaggac gccgccatag gccacgacgt cgatcctgga gcgcgagccg
     3661 taggggtaca gcctgacgaa ggtgccgggg tcgctccgga cgaagacctt ggcctgcagg
     3721 accgagccgt cggcggcgga tcgcttgatg atccagctac cctgggcgta gagcggccc
//
LOCUS       DAOOLN010000034         3759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_29586_length_3759_cov_2.930616, whole genome shotgun sequence.
ACCESSION   DAOOLN010000034 DAOOLN010000000
VERSION     DAOOLN010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29586_length_3759_cov_2.930616"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..1533
                     /locus_tag="PLW80_00775"
     CDS             262..1533
                     /locus_tag="PLW80_00775"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HPB65062.1"
                     /translation="MHKVLSIVLGGGKGTRLFPLTKDRAKPAVPFGGRYRIVDIPISN
                     CINAGFKQIYILTQFNSASLHLHIAKTYVFDNFSNGFAEILAAEQTLTHSGWYEGTAD
                     AVRKNLVHFRTQQPSHYLIVSGDQLYRMDLADMFQRHLESGKALTIACTTVSREDASD
                     FGIMKIDADGRIVEFQEKPGPVKDISDYKIPKSMLKERRGSKEYLASMGIYIFDAGLL
                     ESALENDSNDFGKEIIPEILASRPVNSYIFDGYWEDIGTVRSFYEANLNLTDIAPEFN
                     FYDETSPIYTHRRNLPSSKINYSTMNQAITSDGCIITNASISRSIVGIRTIIETGANF
                     DGVVCMGADYYETEAQKRENAERGVPNIGIGRGSIIKGAIIDKNARIGADCRIGIDAR
                     DRADGEYGDYYIVDGVIVIPKNSVVMPGSII"
     gene            1538..2221
                     /locus_tag="PLW80_00780"
     CDS             1538..2221
                     /locus_tag="PLW80_00780"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HPB65063.1"
                     /translation="MKDAPSAALLVVDVQNYYLNPGSPFYRYSERCWPGCMAYIGRRV
                     ETSVFPAVTRLRQAFSAVGWPTVYLRLCASETDRSDLHRFFRAFNESAARLGFDGAYP
                     LHGEAMADVPDEIAPRPGDAIIDKATYSGFSTPRLEETLRGLGVSAVVMTGLATSQCV
                     DTTARQASERGFTVVHVEDGQADYSQDEHDAALFASRGVCGGHVVESEFLAAAPLRLV
                     RAYVESEAR"
     gene            complement(2237..>3759)
                     /locus_tag="PLW80_00785"
     CDS             complement(2237..>3759)
                     /locus_tag="PLW80_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65064.1"
                     /translation="IGQSLRSYAAADTLVSFREQEGAVFVSVSEDGSGPFARYDEPIA
                     IAGSDEGRKRYVVRAYAEDEFGNRSAEMPRFELMIDRSTLYVDARGRPGASGAPDDPI
                     AGLDDAIEEAQRAGKRFVYVRGGGSISRSVVVSGRLEVRGGFDDSWNEVPGAATAVSV
                     RVPPSSSSYAFVVDGGALTLSSMTFSLKADGAGGLALARSGSLELTRCSIESTGGIEV
                     AAVRSTGADVTISSSTLRSSSAVTGRGIDLSGGRLVVSDSTIACDRSVRLFDAIRVSD
                     AEAFVSGLRIDAEPSHAFSAFSASRSRASIERCIVNVSGGSSSCRAFSAGSSELTASS
                     VYVHAAWKGSVELFSAISGSTMRLAHVTAIADAPAALFASATGSSVEIVNSIASFSGG
                     GSAFLRLDGAAGSVRLASNCLWGFERLAELSSHRRLIATVAELNGAFSSGAAPNRLER
                     PSEIFQSTVKGLYRLSRSSVCVDGATAVPWAAGSDLFGSPRPTGEGAAVSIGAEEL"
BASE COUNT          739 a         1231 c         1178 g          611 t
ORIGIN      
        1 gccgcgcgta gcccgtcgtc gagccgctcc ggcagttcgg cccaggaggc cggggcggcc
       61 ggcacccgcc tcacgaaggc aatattgggg gccagctcgg ggtcggagag gaatctatcg
      121 atggggtcta tggcgtcttc ttcgatcatg gctatactat gatacggtat acggcgagtt
      181 tggtaaatcg atgccaccgc cgccgttggc gtgctatatt gaagcggcgc gaagggctaa
      241 aaaatagaac ggaggagagc gatgcataag gtgctgtcga tcgtgctcgg gggtggcaag
      301 ggtacgaggc tattcccgct taccaaggat agggccaagc cggcggtacc gttcggaggc
      361 cgctacagga tcgtcgacat ccccatatcg aactgcatca acgccggctt caagcagatc
      421 tacatcctga cgcagttcaa ctcggcctcc ctccacctgc acatcgccaa gacctacgtc
      481 ttcgacaact tctcgaacgg tttcgccgag attctcgccg ccgagcagac gctcacccat
      541 tccggctggt acgagggcac cgccgatgcc gtccgtaaga acctggtcca tttcaggacc
      601 cagcaacctt cccattacct gatcgtgtcg ggcgaccagc tctaccgcat ggacctcgcg
      661 gacatgttcc agaggcacct ggaatcaggc aaggccctga ccatagcctg caccaccgtc
      721 tcacgggagg acgccagcga cttcggcatc atgaaaatcg acgctgatgg ccgcatagtc
      781 gaattccagg agaagccggg accggtcaaa gacatctccg actacaagat tccgaaatcc
      841 atgctcaagg aacggcgagg atcgaaggag tacctggctt cgatgggtat ctatatcttc
      901 gacgccggtc tgctcgaatc tgccctggag aacgattcga acgatttcgg taaggagata
      961 attcccgaga tcctggccag ccgcccggtc aactcgtata tcttcgacgg gtactgggag
     1021 gatatcggta ccgtgcgttc cttctacgag gccaatctca acctgaccga catcgcgcca
     1081 gaatttaact tctacgatga gacgagcccg atctacaccc atcgccgcaa cctgccttcg
     1141 tcgaagatca actacagcac gatgaaccag gccatcacct ccgacggctg catcatcacg
     1201 aacgcgtcga tctcgaggtc gatcgtcgga atccgcacca tcatcgagac gggagcgaac
     1261 ttcgatggcg tggtctgcat gggcgccgat tactacgaga ccgaggccca gaagcgggag
     1321 aacgccgagc gcggcgtgcc gaacataggc ataggccgcg gatcgatcat caagggcgcc
     1381 atcatcgata aaaacgcgag gatcggcgcc gactgccgca tcggcatcga cgcgagggat
     1441 cgcgccgacg gcgagtacgg cgactactac atcgtggacg gggtcatcgt aatccccaag
     1501 aactcggtgg tcatgccggg tagcatcatc tagacacatg aaggatgctc catccgccgc
     1561 cctgctcgtc gtcgacgtgc agaactatta cctcaacccc ggctcgccgt tctaccgcta
     1621 ctccgagcga tgctggcccg gttgcatggc gtacataggc cggcgcgttg agacgtcggt
     1681 gttccccgcc gtaaccaggc tcagacaggc cttctccgcc gtcggctggc ccaccgtcta
     1741 cctgcggcta tgcgcctcgg agacggaccg cagcgatctg caccgctttt tcagggcctt
     1801 caacgagtcg gccgcccggc tgggtttcga tggtgcctac ccgctccacg gagaggcgat
     1861 ggccgacgta ccggacgaga tcgccccgcg cccgggcgac gcgatcatag acaaggccac
     1921 ctactccgga ttctcgacgc cgcggctcga ggagacgctc cgaggcctcg gcgtctcggc
     1981 cgtcgttatg actgggctgg ccacgagcca gtgcgtcgat accacggcca ggcaagcgtc
     2041 cgagcgcggc ttcaccgtgg tgcatgtgga ggacggacag gccgactaca gccaggacga
     2101 gcatgacgcg gccttgttcg cctcgagagg cgtctgcgga gggcatgtcg tcgagtccga
     2161 gttcctggcc gcggctccgc tacggctggt gcgagcctac gtggagtccg aggcgcgctg
     2221 aatcgccacg gctcgtttat agctcctcag ccccgatgga cacggcggcc ccctcgcccg
     2281 tcggcctggg cgagccgaac aggtcgcttc cggcggccca aggcacggcc gtcgcgccgt
     2341 cgacgcagac cgacgagcgg gacaggcgat acagaccctt gaccgtggat tggaaaatct
     2401 cggacgggcg ttcaagcctg ttcggagccg cgccagaact gaacgcaccg ttcaactcgg
     2461 cgacggtagc gatgaggcga cgatgggacg atagctcggc gaggcgttcg aagccccaga
     2521 ggcagttcga cgccagccgg accgacccgg cggcgccgtc gaggcgcagg aacgccgagc
     2581 cgccgcccga gaacgaggct atcgagttga ctatctcgac gctcgacccg gtcgccgacg
     2641 cgaacagggc cgccggcgcg tccgctatgg ccgtgacgtg ggccagtcgc atcgtcgacc
     2701 cggatatggc gctgaatagc tcgaccgagc ctttccaggc ggcgtggacg tagaccgacg
     2761 aggcggtaag ctccgaggag ccggcgctga aggcccggca cgacgaggaa ccgcccgata
     2821 cgttgacgat gcatcgctcg atcgaggccc gcgagcgtga cgcgctgaaa gccgagaacg
     2881 cgtgcgacgg ttcggcgtcg atgcgcaggc cggagacgaa ggcctcggcg tcgctcacgc
     2941 ggatcgcgtc gaacagccga accgaccggt cgcaggctat cgtcgagtcc gagacgacga
     3001 gcctgcctcc ggacagatct atacccctgc ccgtgaccgc cgaggaggag cgtagagtcg
     3061 acgacgagat ggtgacgtcg gccccggtgg acctgacggc cgccacctcg atgcctcctg
     3121 tagactctat cgagcaacgg gtcaattcga gcgagcctga gcgcgccagc gcgagccccc
     3181 cggcgccgtc ggccttgagc gagaacgtca tcgacgacag ggtcagcgcc cctccgtcga
     3241 cgacgaaagc gtacgatgac gacgacggag gcactcggac cgaaacggcg gtggccgcgc
     3301 ccggcacctc gttccagcta tcgtcgaagc cacccctgac ctcgagccgg ccgctcacga
     3361 ccacgctacg gctgattgaa ccgcccccac gtacgtagac gaatcgcttc ccggctcgct
     3421 gagcttcctc gatcgcgtcg tcgagcccgg ctatcgggtc gtcgggcgcg cccgaggcgc
     3481 cgggtcggcc gcgagcgtcg acgtacaggg tcgaacggtc tatcatcagc tcgaatcgag
     3541 gcatctccgc ggaacgatta ccgaactcgt cctcggcgta ggcgcgcacg acatagcgct
     3601 tgcggccctc gtccgaaccc gcgatggcga tcggttcgtc gtatcgcgcg aacggacccg
     3661 atccgtcctc ggagaccgat acgaacaccg cgccttcctg ctctctaaag gacacgagcg
     3721 tgtcggcggc cgcgtagcta cgcaggctct ggccgatgg
//
LOCUS       DAOOLN010000035         3736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_29819_length_3736_cov_3.541701, whole genome shotgun sequence.
ACCESSION   DAOOLN010000035 DAOOLN010000000
VERSION     DAOOLN010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29819_length_3736_cov_3.541701"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PLW80_00790"
     CDS             <1..840
                     /locus_tag="PLW80_00790"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HPB65065.1"
                     /translation="GAVLVGRGRGRIGHPAGCLGRAMRASLHGFLSAFTLASRIPAPL
                     RSEPDYSALGFWMPVVGLCAAAVACAGAWLGSLAFGPGPLAALASIVAQYLAFNLFHL
                     DGLLDTADAAGVNGDAERRRTVLKDPRVGSFALFAGFAALAARLGAVTALAERGGPVF
                     WGALCLAPVAGRFGSMLVTAFCEPYPGGGLAAMIGRPSPVSSTVGYVVAAAPAALLFG
                     AAYGPIGAAAAMLAGGLAAVATAAFVGYWYGKRLDGYSGDALGAAVELGELAVLLLAV
                     AVA"
     assembly_gap    1077..1176
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1297..2856)
                     /locus_tag="PLW80_00795"
     CDS             complement(1297..2856)
                     /locus_tag="PLW80_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HPB65066.1"
                     /translation="MKRFALVLAVVAAIFAVGCVSQPAAPAGGEVKIAVLHVNDTHGH
                     PIAFANSPHLAVGGLPAMAMFVGQTRAEYDNVLVLDAGDYNTGLSESNFFKAEPDVVG
                     RNMIGFDAVALGNHEFDNEISVLEAQKALAEFPMISANVKTKAGAYVADAPYIVKEFQ
                     GVKVGIFGLITKEAETVGNPAIVKDLVFADEIETAKEMVKILREKEKVDVVVALVHLG
                     MDDVYVPGSKGLAAAVTGIDLIVDGHSHTLPAAPVVVNGTPIVQAGQWGMQVGKGVLT
                     VKDGKVAAFDWEAVTINHNDKDKRPVGPQFEQHPLIQPVLSDYADRVEGLLKEKIGVA
                     AAAFPNAMTRKAETAIGDLVTDAMKFITKAQGVDFAMNNGGGIRADLPAGDVTLKSIY
                     TVLPFDNSVTVIKLKGTDVQAMFDYIATTIGKGAYAQVSDGVSYTINVKAGTCENILI
                     GGKPLDPAKVYTIATNSYLATGGDGYVMFKNALSSYETSMFQRDALIAYVRQLGKDLT
                     PVIAGRVTIVE"
     gene            3102..>3736
                     /locus_tag="PLW80_00800"
     CDS             3102..>3736
                     /locus_tag="PLW80_00800"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HPB65067.1"
                     /translation="MRLRRSSGVLLHPTSLPGPFGIGDLGPEAGRFVDWLAEAGQSVW
                     QVLPLGPTGYGDSPYAPFSSFAGNILLLSPETLRRDELLRDEELEAARDTRGGPVDYG
                     RVIPAKERLARLAAERLLSSGRFASELDAFRLANAGWLDDYCLFTVIKRERDVAAAAA
                     GVDDSSWNAYWPRTLARREPEAIAAFRAGHGRELSLIEAEQFLFRRQWDELR"
BASE COUNT          542 a         1165 c         1276 g          653 t
ORIGIN      
        1 ggagctgttc tcgtgggccg aggccgaggc cggatcggcc atcctgcggg atgcctgggg
       61 cgcgctatga gggcttccct gcacggcttc ctgagcgcgt tcaccctggc ctccaggata
      121 ccggccccgc tccgatccga gccggactat agcgcgctcg gattctggat gcccgtggtc
      181 ggcctctgcg cggcggccgt cgcctgcgcc ggcgcctggc tcggctcgct ggccttcggc
      241 ccggggcccc tggcggcgct cgcctcgatc gtcgcccaat acctggcctt caacctgttc
      301 cacctggatg ggctcctcga tacggccgac gccgccggcg tgaacggcga cgcggagcgg
      361 aggcgcacgg tactcaagga tcctcgcgtc ggctcgttcg ccctgttcgc cggtttcgcc
      421 gccctggcgg cgaggctcgg cgccgtcacg gcgctggccg agcgcggcgg cccggtgttt
      481 tggggcgccc tctgcctagc gccggtcgcc ggacgcttcg gttcgatgct cgtaacggcc
      541 ttctgcgagc cttacccggg cggcggcctc gccgccatga tcgggcgccc gtctccggtg
      601 tcatcgactg tcggctacgt ggtggcggct gcgccggccg cgctactgtt cggcgcggcc
      661 tacggaccga tcggcgcggc cgccgccatg ctggccggag gcctagcggc cgtggccacg
      721 gcggccttcg tcggatattg gtacggcaag cgcctcgacg gctactcggg cgacgcgctc
      781 ggagccgccg tcgagctcgg cgagctggcc gtattgctac tagccgtcgc cgtcgcctag
      841 ctaccgaact gcgcctaacg agcaggccga tcttagtgtc acgaatctta gcggtttcat
      901 tctataggcg gatgacgact atctcatagc ccgtacgcag acgccgggtc tccatactga
      961 tggccgtggt gcaatagaaa tgggtacacg tgcatcaccc acaagcttca tgacaccata
     1021 agcgccccgg gctagcgacg aagccggcgc cgcgccgagc gaaccgggct tttcgtnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgct ccgtgcatgt cgaggcgcgc
     1201 cccgggctag cgacgaagcc gacgccgcgc catggacgaa aagaaggccg ccccgagggg
     1261 cggccttatc atacgaagtc tatcgtcgac ctggaattac tcgacgatgg tgacgcggcc
     1321 ggcgatgacc ggggtcaggt ccttgccgag ctggcgaacg taggcgatga gggcgtcgcg
     1381 ctggaacatg ctggtctcgt aggaggacag cgcgttcttg aacatgacgt agccgtcgcc
     1441 gccggtcgcc aggtaggagt tggtggcgat ggtgtagacc ttggcggggt cgaggggctt
     1501 gccgccgatg aggatgttct cgcacgtgcc ggccttcacg ttgatcgtgt agctgacgcc
     1561 gtcggagacc tgggcgtagg cgcccttgcc gatggtggtg gcgatgtagt cgaacatggc
     1621 ctgcacgtcg gtgcccttga gcttgatgac cgtgacggag ttgtcgaagg gcagcaccgt
     1681 gtagatcgac ttgagggtga cgtcgccggc gggcaggtcg gcgcggatgc cgccgccgtt
     1741 gttcatggcg aagtcgacgc cctgggcctt ggtgatgaac ttcatcgcgt cggtgacgag
     1801 gtcgccgatg gcggtctcgg ccttgcgggt catcgcgttc gggaaggcag cggcggctac
     1861 gccgatcttc tccttgagca ggccctctac cctgtcggcg tagtcggaca gcacgggctg
     1921 gatgagcggg tgctgctcga actgcgggcc gaccggccgc ttgtccttgt cgttatggtt
     1981 gatggtgacg gcttcccagt cgaaggcggc gaccttgccg tccttgacgg tcaggacgcc
     2041 cttgccgacc tgcatgcccc actggccggc ctggacgatc ggggtgccgt tgaccaccac
     2101 gggggcggcg ggcagggtgt ggctgtggcc gtcgacgatc aggtcgatgc cggtgacggc
     2161 cgcggcgagg cccttggagc cgggcacgta gacgtcgtcc atgccgaggt ggacgagggc
     2221 gaccacgacg tcgaccttct ccttctcgcg gaggatcttg accatttcct tggcggtctc
     2281 gatctcgtcg gcgaacacga ggtccttgac gatggccggg ttgccgacgg tctcggcttc
     2341 cttggtgatc aggccgaaga tgccgacctt gacgccctgg aattccttga cgatatacgg
     2401 agcgtcagcg acataggcgc cggccttggt cttgacgttg gcggagatca tcgggaactc
     2461 ggcgagggcc ttctgggcct cgagcacgga gatctcgttg tcgaactcgt ggttgccgag
     2521 ggcgactgcg tcgaagccga tcatgttgcg gccgacgacg tccggctcgg ccttgaagaa
     2581 attcgactcc gagaggccgg tgttatagtc gccggcgtca agaacgagca cgttgtcgta
     2641 ctcggcgcgg gtctggccga cgaacatggc catggcgggc aggccgccga ccgcgaggtg
     2701 cgggctattg gcgaaggcga tcgggtgacc gtgggtgtcg ttcacgtgca gaacggctat
     2761 cttgacctct ccgccggccg gagccgcggg ctggctgacg cagccgaccg cgaagatggc
     2821 ggcgaccact gccagtacca aggcgaaacg tttcatagaa cctcccttaa gaattggaac
     2881 ggtgatggga cgcgaaacgg cgaccgacga tcgccgttca ggccgataat ggtaccaccg
     2941 tatccgacta ggcgcaagcg tgccagatac ttttctgtta aactcgtttc ataacacaaa
     3001 acgcgcttgt agcggcggca ccggaactcg gcgagccggc gtcgggccgc ggcccctttg
     3061 acacgcggcc cggacgagcc tatacctaaa gcggagggcg gatgcgactg agacgatcga
     3121 gcggagtact actgcacccc acctcgctac ccgggccgtt cggcatcggc gacctcggcc
     3181 ccgaggccgg ccgcttcgtc gactggctcg ccgaagcggg ccagtcggtc tggcaggtac
     3241 tgccgctcgg gccgacgggc tacggcgact cgccatacgc gccgttctcg agcttcgccg
     3301 gcaacatcct cctcctgtca ccggagaccc tgaggcgcga cgagctcctc cgcgacgagg
     3361 aactcgaggc ggcgcgcgat acccgcggcg ggccggtgga ctacgggcgc gtcatcccgg
     3421 ccaaggagcg cctggctcgg ctggccgctg aacggctcct ctcgtccgga aggttcgcct
     3481 cggagctcga cgccttcagg ctggcgaacg cgggctggct cgatgattat tgcctattca
     3541 cggttatcaa gcgcgagcgc gacgtcgcgg cggcggcggc cggcgtcgat gactcgtcgt
     3601 ggaacgcgta ctggccgcgc accctcgcca ggcgcgagcc ggaggccatc gcggcctttc
     3661 gcgctgggca tgggcgcgag ctgtcgctga tcgaggccga gcagttcctg ttccgccggc
     3721 aatgggacga actacg
//
LOCUS       DAOOLN010000036         3707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_30142_length_3707_cov_2.746440, whole genome shotgun sequence.
ACCESSION   DAOOLN010000036 DAOOLN010000000
VERSION     DAOOLN010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30142_length_3707_cov_2.746440"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="PLW80_00805"
     CDS             <1..1117
                     /locus_tag="PLW80_00805"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HPB65068.1"
                     /translation="MAEASYTVLVVDDSGANRAFLSSILEEDGYRVAQAADGEAGLVA
                     AATERPVLILLDVVMPRLDGFETLKRLKADPATRRISVIMLTSLGDQESKLKAFDYGA
                     VDYIIKSANEAEVRARVRVHVQLALANEELIEARAASLRQISEAQRSLLVKPTELPEA
                     RFSVYYRSLHEAGGDFYDVVPVSDGVYFYLIADVAGHDVGTSYVTPAVKVLLKQSATP
                     AHSVEETLAYLNGVLAKTVLEDNYLTAFALRVNRRAGKVVFAAAGHPPALFIPKDGSP
                     RLAERENPFIGMLDDSSYEATSLDVAEGDRFILYTDGLIEGGGDDKASWTDAKGGLVT
                     VAERLRGLPLEELPLAFVKAMGADKADDDVAVLAVEI"
     gene            1128..1580
                     /locus_tag="PLW80_00810"
     CDS             1128..1580
                     /locus_tag="PLW80_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HPB65069.1"
                     /translation="MRLWTLDPRYLDAKGLVAAWREALLAQAVLEGRTVGYARHPQLE
                     RFRRAPDPAASLGRFLEALAGEAGARGYTFDASKIHGLDRPDPAPLPVTLGQVAYELE
                     LLRSKLNARDPELATGLPASVPVALNPVFVSTPGPVESWERVRPDVAR"
     gene            1646..3226
                     /locus_tag="PLW80_00815"
     CDS             1646..3226
                     /locus_tag="PLW80_00815"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB65070.1"
                     /translation="MDTQTLTLAVAMAGLSIVAVTALFVAFRHERSLAIFSLGFLSAM
                     VGFVLLIGQNKPHDWASFILANALIVFYQLCMAWGVRSFFGVGKGWPARFWAYMAAWL
                     ALLTIGTYVYDSFAVRAIGASVFIIAASVEFLVAVRGVGEGIPPIIRRAAWGVVLAFI
                     ACHLVRVGLVAANANARLMDDAFVNAYTFSFTLIFSIAMAGLILIIDVSHLVSLLEKK
                     NAKLESLAMCDGLTGLRNRHALELKLEGELERASRYRVPLSVILFDIDYFKRVNDTWG
                     HQAGDGVIKRMAELTSALLRQPDDLFRWGGEEFMLVSPHTDRAGATALAEKLRAAIEA
                     ESFPEAGTVTASFGVAEWKPGEDCDEWFKRVDRALYRAKNTGRNRVVGIDAGEAAPAA
                     MVRIDWKAEWSSGNRLIDGEHRELLGLSNNLIDLSLVGADATAMLEAFDALIGHIQKH
                     FGDEERVLAELVYPGLAGHALLHAKLVSESLELRAKVEAGELDHGALFDFLVDKVVVE
                     HLVREDSQFFEYTHRPSA"
     gene            complement(3263..>3707)
                     /locus_tag="PLW80_00820"
     CDS             complement(3263..>3707)
                     /locus_tag="PLW80_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPB65071.1"
                     /translation="QFTKLEMFVYCLPEDSDRLHEELRLIEEEIFAGLGLPFRVVDTC
                     TGDLGAPAYRKWDLEAWMPGRNGGEWGEVTSTSNCTDYQARRLNIKYKDDDGRNKYVH
                     TLNGTAVAISRALVALLENYQQADGSVMMPAVLVPYVGFEVIRKK"
BASE COUNT          610 a         1217 c         1209 g          671 t
ORIGIN      
        1 tatggccgag gcttcgtata ccgtgctcgt ggtcgacgat tccggcgcca acagggcctt
       61 cctttccagc atactggagg aggatggcta ccgcgtcgcg caggctgccg acggcgaggc
      121 cggcctggtc gccgccgcga cggagcggcc ggtcctgatc ctgctcgacg tggtgatgcc
      181 taggctcgat ggcttcgaga cgctcaagcg gctcaaggcc gacccggcca ccaggcgcat
      241 ctccgttatc atgctgacga gcctcggcga ccaggaatcc aagctcaagg ctttcgacta
      301 cggcgccgtc gactatatca tcaagtccgc caacgaggcc gaagtccggg ccagggtgcg
      361 cgtccacgtg cagctagcgc tcgccaacga ggagctgatc gaggccaggg ccgccagcct
      421 gcggcagatt tccgaggccc agagatccct cctggtcaag ccgaccgagc tgccggaggc
      481 ccgcttctcc gtctactatc gctccctgca cgaggctggc ggcgatttct acgacgtcgt
      541 gccggtgtcc gacggcgtct atttctacct gatagcggat gtggccggcc atgacgtcgg
      601 tacgagctac gtgacgccgg ccgtcaaggt gctcctgaag cagagcgcga cgccggccca
      661 ctcggtcgag gagacgctgg cctatctaaa cggcgtgctg gccaagaccg tgctcgagga
      721 caattacctg acggctttcg cactacgcgt caaccggcgc gcgggcaagg tcgtcttcgc
      781 cgccgcgggc catccgccgg ccctattcat ccccaaggac ggctcgccac ggctggccga
      841 gcgggagaac ccctttatcg gcatgctgga cgactccagc tacgaggcga cgtcgctcga
      901 cgtcgcggag ggcgatcgct tcatcttgta taccgacggc ctgatcgagg gcggcggaga
      961 cgacaaggct agctggaccg acgccaaggg cggcctcgta acggtggcgg aacgccttcg
     1021 cggcttgccc ctggaggagt tgccgctcgc cttcgtcaag gccatgggag ccgacaaggc
     1081 ggacgacgac gtggccgtgc tggcggtaga gatctaggcg ccgcgcgatg cgcctgtgga
     1141 ccttggaccc gcgctacctc gacgccaagg gcctcgtcgc cgcctggcgc gaggcgctac
     1201 tcgcccaggc cgtgctcgag ggtcgtaccg tcggctacgc ccgccatccg caactcgagc
     1261 gcttcaggcg ggcgcccgac cccgcggcgt cgctcggccg cttcctcgag gcgctcgccg
     1321 gcgaggccgg tgctcgcggc tacacgttcg atgcctccaa gatccacggc ctcgatcgcc
     1381 ctgacccggc gccgcttccc gtgaccctcg ggcaggtcgc ctacgagctc gaactactca
     1441 ggagcaagct taacgcccgc gacccggagc tggctacggg gctacccgcc tcggtaccgg
     1501 tggcgctcaa cccggtcttc gtatcgacgc cggggcccgt cgagtcctgg gagcgagtcc
     1561 ggcccgacgt ggcgcgttaa ggccggcgcg gtacgcgaga gccgacggct ggattccgaa
     1621 tcgctaataa actactatga tcgccatgga tactcaaacg ttgacgctcg ccgtcgccat
     1681 ggccggacta tccatcgtag ccgtcacggc gttattcgta gcctttcgtc acgagcgtag
     1741 cctcgccatc ttctccctcg gctttctctc ggccatggta ggcttcgtac tgctgatcgg
     1801 ccagaataag ccccatgact gggcctcgtt catactcgcc aacgctctga tcgtcttcta
     1861 ccagctctgc atggcctggg gcgtcaggag cttcttcggg gtcggcaagg gctggcccgc
     1921 ccggttctgg gcctacatgg ccgcatggct tgctctgttg accataggta cctacgtcta
     1981 cgattcattc gccgtacggg ccatcggcgc ttcagtcttc atcatcgcgg ctagcgtcga
     2041 gtttctcgtc gccgtccggg gcgtcggaga ggggataccg cctataatcc ggcgcgccgc
     2101 ctggggcgtc gtactggcgt tcatcgcctg ccacctggtt cgcgtcggac tggtcgcggc
     2161 taacgccaac gccagactta tggatgacgc tttcgtcaac gcctatacgt tctcgttcac
     2221 cctgatcttc tcgatagcga tggcggggct catcctgatc atcgacgtgt cccatctcgt
     2281 gagcctgctt gagaagaaga acgctaagct cgagagcctg gctatgtgcg acgggctgac
     2341 gggcctgcgg aaccggcatg cgctggagct gaaactcgag ggagagctgg aacgcgcctc
     2401 gaggtatcga gtgcctttgt cggtcatact cttcgatatc gactacttca agcgagtcaa
     2461 cgatacctgg ggacaccagg cgggcgacgg ggtgatcaag cgtatggccg aactgacgag
     2521 cgcgctgtta cgccagcctg acgacctgtt ccgctggggc ggcgaggaat tcatgctcgt
     2581 gtcgccgcac accgataggg ccggcgcgac ggcgctcgcc gagaagctgc gcgccgccat
     2641 cgaggccgag agcttcccgg aggccggcac ggtgacggcc agtttcggcg tcgcggagtg
     2701 gaagcccggc gaggattgcg acgaatggtt caagcgggtg gaccgggcgc tctaccgggc
     2761 caagaatacc ggacgcaatc gcgtcgtggg catcgacgcc ggagaggccg cgccggcggc
     2821 gatggtgcgc atcgactgga aggccgagtg gtcgtccggc aatcggctca tcgacggcga
     2881 gcaccgcgag ctgctcggcc tatcgaacaa cctgatagac ctgtcgctcg ttggtgccga
     2941 cgcgacggcc atgctcgagg cgttcgacgc cctcatcggc catatccaga agcactttgg
     3001 cgatgaggaa cgggtcttgg ccgagctcgt ctatccgggg ctcgcagggc acgcgctcct
     3061 gcacgccaag ctggtatcag aatcgctcga gctgcgcgcc aaggtcgagg ccggagaact
     3121 cgaccacggc gccctgttcg acttcctcgt cgacaaggtc gtcgtcgagc acctggtccg
     3181 agaggacagc cagttcttcg agtacacgca tcggccttcg gcctgagcgt cgccggatcc
     3241 gctcagaccg taggccggcg gctcacttct tgcggataac ctcgaagccg acgtacggta
     3301 cgagaacggc cggcatcatg acgctgccgt cggcctgctg gtaattctcg agtagggcga
     3361 cgagggcccg cgagatggcg acggccgtgc cgttgagggt gtgcacgtat ttattcctgc
     3421 catcgtcgtc cttgtacttg atattgagcc gccgggcctg gtagtcggta cagttggagg
     3481 tactcgtcac ctcgccccat tcgccgccgt tgcggccggg catccaggcc tcgaggtccc
     3541 acttgcggta ggccggcgcg ccgaggtcgc cggtgcaggt gtccaccacg cggaagggca
     3601 gtccgagccc ggcgaagatt tcctcctcga tcaggcgcaa ctcctcgtgc aggcggtcgg
     3661 agtcctcggg caggcagtag acgaacatct ccagcttggt gaactgg
//
LOCUS       DAOOLN010000037         3673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_30514_length_3673_cov_3.224986, whole genome shotgun sequence.
ACCESSION   DAOOLN010000037 DAOOLN010000000
VERSION     DAOOLN010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30514_length_3673_cov_3.224986"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLW80_00825"
     CDS             complement(<1..258)
                     /locus_tag="PLW80_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65072.1"
                     /translation="MRKRNLALLALFALAIAAVVVVPKLAGSGSQAAAMPPGARPTSG
                     AGPRPSGAPGRGAAVATEFSVRTVRAERTTLQDYIETNGDVV"
     gene            complement(261..1526)
                     /locus_tag="PLW80_00830"
     CDS             complement(261..1526)
                     /locus_tag="PLW80_00830"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPB65073.1"
                     /translation="MRIPITLAVALLALAATADASAQDAFNIDEAVSIALEGNRSLAR
                     AKIDREAASRALSLAWNDLVPSLSVGASASASDGRGSVGGSATASLSLSAESLHARSQ
                     AELAYVTAELAYETARRDVELSVRTAFYALLLKQEKIRLAEQNAATARKSYEQAEARR
                     KAGVAPELDALSALVRLEKLGPALESARMDYETSLDSFRQLLGLEPGAPVTLTGSLDS
                     STVAVRAAAAVDPAAAAVSSPSVRTMERSKASAEASLAKARRAVYSPSLSLSASYRPS
                     RTDASLSGSGSLSASVSLGLDDMLPWSDARLAVASAEDAVAKIDSQLAEASARAVSGA
                     ASLQRTIAQAAKSLETGRLSESLAARTYALTEEAYRFGTKDLLSLQSAQDSLAEARVS
                     VSESAYALIVALLNLEYALGEPFGSLGRD"
     gene            1718..2449
                     /locus_tag="PLW80_00835"
     CDS             1718..2449
                     /locus_tag="PLW80_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB65074.1"
                     /translation="MKAKVLIIEDVAELSDLVALYLSKEGMETRRAESAEAALAMLRA
                     VGAEAFSPDIIILDINLPGMDGFEFLGEYRKAGAAPVLIVSARDADEDIIAGLGHGAD
                     EFVTKPFSPRVLVARVRAMLRRAQEAPSARDARSIRFGPYSLDLEACVLRRGEERVAL
                     SAKEYAVLAFLAANPGRPLDPETIYSSVWKNAYGDVTAVAVYIQRLRRKLEADPSSPR
                     HIETVYGMGYRFTLDPERGPDGGGR"
     gene            2446..>3673
                     /locus_tag="PLW80_00840"
     CDS             2446..>3673
                     /locus_tag="PLW80_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPB65075.1"
                     /translation="MRIRSQFYILVFGIVVVPLLVFAGLLAMQEGRESPVVEVPGYDE
                     VAELAGGSFDRDSWERLADYVAHKPPTMEFTVLDPERRVVFSTMSGHPVGDLVSDEAL
                     LGYMRETSGDYFYQIDSPVRSSDGGLLVLTRIERSERLWPPNPFSRFFSSFAVLLAAL
                     FLFSAGMAVLIARSITQSVLALDAATRRIAAGELERNVEVRGSNEITSLASSLNAMRL
                     ALKDDLARRSRFIMGVTHDLKTPLALIKGYAEAIGDGMDDGADTRERYVGIIGLKVDQ
                     LAGMIDDLIDFVRVDSGEWRERLERAPLAPFLRGLCRRLEEDAVLLGRRAESSIALPD
                     DLAVPFDERLITRALENIVNNALRYTEPGGLVRVAAWLERGADGGDRVAIEVSDDGPG
                     IGEGELPLIFDPFYRGS"
BASE COUNT          545 a         1264 c         1265 g          599 t
ORIGIN      
        1 gacgacgtcg ccgttcgtct cgatatagtc ctgcagggtc gtccgctcgg ccctgacagt
       61 gcgcaccgag aactcggtgg ctaccgcggc gcctcggccc ggcgctccgg aaggacgggg
      121 gccggcgccg ctcgtcggcc ttgccccggg tggcatcgcg gccgcctggc tccctgagcc
      181 ggctagcttc ggcacgacga cgaccgccgc gatggcgagc gcgaacagcg cgagtaaggc
      241 taggttgcgt ttcctcatgt tcaatccctc ccgagcgagc cgaacggctc gcccagcgcg
      301 tattcaaggt tcagtagagc gacgatcagc gcgtacgcgc tttccgatac ggataccctg
      361 gcttcggcca gactgtcctg ggcgctctgg agcgacagga gatccttggt tccgaagcgg
      421 taggcctcct cggtcagggc gtaggtccgc gcggccagcg actcgctcag gcgccccgtc
      481 tcgagcgact tggccgcctg ggctatcgtg cgctggagtg aggcggcgcc cgagacggcc
      541 ctggccgaag cctcggccag ctggctgtcg atcttggcca cggcgtcctc ggccgaggct
      601 acggcgagcc tcgcatccga ccagggcagc atatcgtcga ggcccaggct gaccgaggcc
      661 gacagggagc ccgagccgct cagggacgcg tcggtacgac tcggcctata cgaggccgat
      721 aggctcagcg acggcgagta caccgcgcgc ctggccttgg cgaggctggc ctccgcgctg
      781 gccttggagc gctccatggt tctgaccgac ggcgacgaga cggcggcggc cgcgggatcg
      841 acggcggcgg cggcccgtac cgctaccgtc gacgagtcga gcgagccggt cagcgtcacg
      901 ggcgcgccgg gctcgaggcc gagcagctgc ctgaacgagt cgagcgacgt ctcgtagtcc
      961 atcctggcgc tctcgagcgc aggcccgagc ttctcgagcc tgacgagggc cgacagcgcg
     1021 tcgagttccg gggccacgcc ggccttccgc ctggcctcgg cctgttcgta gctcttcctg
     1081 gccgtcgccg cgttctgctc cgccaggcgt atcttctcct gcttcaagag cagcgcgtag
     1141 aaggcggtcc gtaccgacag ctcgacgtcg cgcctggccg tctcgtacgc cagctcggcc
     1201 gtcacgtagg ccagctccgc ctgggaccgc gcatggagcg actcggcgga caggctcagg
     1261 ctcgcggtcg cggagccgcc gacgcttccc cggccgtcgc tagcgctggc cgaggcgccg
     1321 accgataggg acggtacgag gtcgttccac gccagcgaca gggccctgga agcggcctcc
     1381 ctatcgatct tggccctggc caggctccta ttgccctcga gcgcgatcga gacggcctcg
     1441 tcgatgttga aagcatcctg ggcgctcgcg tcggcggttg ccgcgagcgc gagcagggcc
     1501 acggccagcg ttatcggtat tcgcatctat ccctccatgt catcctatcg ttccgaggat
     1561 accctatcgt cgagcctgag catacgtcca aaccggttcg cggcctatag acgcgccatg
     1621 aaaggcacat aattttttgt tataggcgga ggcatcgccg ccgctcggcg ttgcccggcg
     1681 ctcgggccgc gcgtatactc cgaaccgtgg ggtgagcatg aaggcgaagg tactgatcat
     1741 cgaagacgtg gcggagctgt cggacctcgt ggctctgtac ctgtccaagg aaggcatgga
     1801 gacccggcgg gccgagtccg ccgaggccgc cctcgccatg ctccgggccg tcggcgccga
     1861 ggcgttctcg ccggatatca tcatcctcga catcaacctg ccaggcatgg acggcttcga
     1921 gttcctcggc gagtaccgca aggccggggc ggcccccgtg ctgatcgtat cggccaggga
     1981 cgccgacgag gacatcatcg cgggcctcgg ccacggcgcc gacgagttcg tgaccaagcc
     2041 gttctcgccg cgggtgctcg tggcccgtgt gcgagcgatg ctcaggcgcg cccaggaggc
     2101 cccgtcggcg agggacgcgc ggtcgatacg cttcggcccg tactcgctcg acctggaggc
     2161 ctgcgtgctc aggcgaggag aggagcgcgt cgctctatcg gccaaggagt acgcggtgct
     2221 cgcgttcctg gccgccaacc ccggccggcc cctcgacccg gagaccatct actcgtcggt
     2281 atggaagaac gcctacggcg acgtgaccgc ggtggccgtc tatatccagc ggctacgcag
     2341 gaagctcgag gccgacccct cgtcgccgcg gcacatcgag acggtctacg gcatgggcta
     2401 ccggttcacc ctcgatccgg aacgcgggcc ggacggaggc gggcgatgag gatacgaagc
     2461 cagttctaca tcctcgtatt cggcatcgtc gtcgtgccgc tgctcgtgtt cgcgggcctc
     2521 ctcgcgatgc aggagggccg cgagtcgccg gtcgtcgagg tgcccggtta cgacgaggtg
     2581 gccgagctgg ccggcggctc cttcgaccgg gattcctggg agcgcctcgc cgactacgtg
     2641 gcccataagc cgccgaccat ggagtttacc gtgctcgacc ccgagcgccg ggtggtcttc
     2701 tcgacgatga gcgggcatcc ggtgggcgac ctcgtctccg acgaggccct gctcggctac
     2761 atgcgggaga cgagcggcga ctacttctac cagatcgact cgcccgtgcg atcgagcgac
     2821 ggaggcctgc ttgtcctgac caggatagag cgctcggagc gcctctggcc gccgaacccg
     2881 ttcagccgct tcttctcgtc gttcgcggtg ctgctcgcgg cgctgttcct gttctcagcg
     2941 gggatggccg tgctgatcgc caggtccatc acccagtcgg tcctggcgct cgacgccgcg
     3001 acccgacgca tagccgccgg ggagctggag cggaacgtcg aggtccgagg ctcgaacgag
     3061 atcacctcgc tcgcctcgtc gctcaacgcg atgcggctcg cgctcaagga cgacctggcc
     3121 aggcgctctc gcttcatcat gggcgtgacc cacgacctca agacgccgct cgcgctgatc
     3181 aagggctacg ccgaggcgat aggcgacggc atggacgacg gcgcagatac ccgcgagcgc
     3241 tacgtcggca tcatcggctt gaaggtggac cagttggccg gtatgatcga tgacctgata
     3301 gacttcgtca gagtcgactc gggcgagtgg cgggaacgcc tcgagcgcgc gccgctggcg
     3361 cccttcctgc gcggcctctg ccgtaggctc gaggaggacg ccgtgctgct cgggcgccgg
     3421 gcggagtcgt cgatagccct gcccgacgac ctggccgtgc ccttcgacga acgcctgatc
     3481 acgagggcgc tcgagaacat cgtcaataac gcgctacgct acaccgagcc gggcgggctc
     3541 gtgcgcgtcg cggcctggct cgagcgcggc gctgatggcg gcgaccgcgt agcgatcgag
     3601 gtatccgacg acggccccgg catcggcgag ggcgagctgc cgctcatctt cgacccgttc
     3661 taccgcggct cgg
//
LOCUS       DAOOLN010000038         3646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_30794_length_3646_cov_2.900306, whole genome shotgun sequence.
ACCESSION   DAOOLN010000038 DAOOLN010000000
VERSION     DAOOLN010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30794_length_3646_cov_2.900306"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2345
                     /locus_tag="PLW80_00845"
     CDS             <1..2345
                     /locus_tag="PLW80_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPB65076.1"
                     /translation="LAVTRLEDIAEIDARYDLPSRVFLLGDKPAIQITLTPEDGGNLN
                     RMTEELVVIMEEAKASGRLPADVGFELYLDPAKYINRSISNVVQSALLGGLLAVLIVF
                     LILGELRNTLIIALTVPVTIVLSFILMSLFGLTINLISLGGLALAVGMIVDPTIVVME
                     NIHRWRAEARRAGDASGSAAGKDLVADATRQVRGPVIASALTSVAVFLPLTFTAPLAA
                     AILGDQAKTVVFSLLVSLLVSLSFVPVAAYLLFGTGKRAALSEPRGYAKIADAAMDRL
                     VEAYRRALRAVIRTRPRAATFLTLAFAALVVSVGLLLPRVPKEMLPTPSSDRVVVFFR
                     HGDYTTAPEVIERLMPELRERIAGAMDPIAYKDFASVSGRMNQIFVDLESTEYTDEAI
                     VRLEAEFESEGQWYFSIASWDPAALPLPQTNDLRLSVHGPDPTVKVALLDEMQRALND
                     SRLYGRTYARPSPALTREVALETRPGAFDGFPGLGEASMATLVRRSLAGSAGLSLSDG
                     SSEVSVSATYPDGLLDSRDELARFLVPWKGTGVPLKHFFDFEESETVSQVYSENGEAA
                     YRLYANISGEASDAVRLEKEAAARRLFDSAIEPPAGYGYSFDNPRGELDEAISSLLAA
                     LAISIGLVYLLLAFQYDSFGVPLAILVAVPFGFVGVALSLFAFRSTLNLNSLLGTILL
                     CGVVVNNSIIMIDFYLKTRKNHQDNASAIEAAAALRLKPILITTLTTVFGMLPIALGM
                     GSGSNILQPLGIAVSGGLAISTLLTLFAVPALLRFWRFPA"
     gene            complement(2355..>3646)
                     /locus_tag="PLW80_00850"
     CDS             complement(2355..>3646)
                     /locus_tag="PLW80_00850"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPB65077.1"
                     /translation="SGMMVDYMRFLPKRYLEEFFLYSSQFVLFFIIILFTSPAIIRPS
                     TSALISLIVFLLLQIALLARFGHRPPARFFFSLMTPLGYTTILTLSKSAQGIDMASFF
                     LWATSLYLGGIQALAILFRRKWPKRIAEIFLSLGTILSFIFFYFYLDLRLTSTKAYEA
                     GELGYAALVSSLKLSNFGPAFGEFAKTLQHVFFMFGAVMFGSFQVADKIKQVSMKARL
                     DQVYSIVEPPSAEPSMFDQVSDGENREVVILYADLWNFTPIAEKEGARATIGLLNRYY
                     ALWDIVARKHGGVVEQFVGDTAIVVFGLMGEKDAADQAVSCAFDFLYEFSHLQEDLAA
                     HRLPLVKHIGIGVHAGKVAVGELSSASGVQRVGVVGEAVNIAARLDSLCREFKQDLLI
                     SQPVYKQLSLKPQSRFQPMGEVLFRGKTQPQAVYGKK"
BASE COUNT          658 a         1192 c         1155 g          641 t
ORIGIN      
        1 gcctggccgt cacccggctg gaggacatag cggagatcga cgcgcgctac gacctgccgt
       61 cgagggtctt cctgctcggc gacaagccgg ccatccagat cacgctgacg cccgaggacg
      121 gcggcaacct gaaccgcatg accgaggaac tagtggtaat aatggaagaa gccaaggctt
      181 ccggccgcct cccggccgac gtcggcttcg agctgtacct ggacccggcc aagtacatta
      241 accgctccat tagcaacgtc gtccagtcgg ctctgctcgg cggcctcctg gccgtcctga
      301 tcgttttcct gatcctggga gagctgcgga atacgctgat catcgcgctg acggtgccgg
      361 ttaccatcgt gctctccttc atcctgatgt ccctgttcgg cctgaccatc aacctcatat
      421 cgctcggcgg cctggcgctg gccgtcggca tgatcgtcga cccgaccatc gtggtcatgg
      481 agaatatcca ccgctggcgc gccgaggcga ggcgcgccgg tgacgcgagc ggttcggcgg
      541 cgggcaagga cctggtagcc gacgccacca ggcaggtacg aggccccgtc atagcgtcgg
      601 ccctgacctc ggtggcggtc ttcctcccgc tcaccttcac cgcgccgctc gcggcggcca
      661 tcctcggcga ccaggccaag accgtggtgt tctcgctact cgtctcgctg ctggtctcgc
      721 taagcttcgt gccggtagcc gcgtacctac tcttcggtac ggggaaacgc gcggcgctct
      781 ccgagccgcg cggctacgcg aagatagccg atgccgccat ggatcgactc gtggaggcct
      841 atcgacgcgc gcttcgcgcc gtgatacgaa ccaggccgag ggcggccacc ttcctgactc
      901 tagccttcgc ggcgctcgtg gtatcggtag ggctcctctt accgcgcgtg cccaaggaga
      961 tgctgccgac gccgagctcc gaccgcgtag ttgtcttctt ccggcacggc gactacacga
     1021 cggcacccga ggtgatagag cggctaatgc cggaactgcg cgaacggata gccggcgcga
     1081 tggacccgat agcgtataaa gacttcgcca gcgtctccgg tcgaatgaat cagatcttcg
     1141 tcgacctcga gagcacggaa tataccgacg aggcgatcgt caggctcgag gcggaattcg
     1201 agtccgaggg ccagtggtac ttcagcatag cctcgtggga ccccgccgcc ctgcccctgc
     1261 cgcagacgaa cgacctcagg ctcagcgtcc acggcccgga ccccacggtc aaggtcgccc
     1321 tcctcgacga gatgcaacga gcgctcaacg actcgaggct gtacggccgg acctacgcga
     1381 gaccctcccc ggctcttacg cgggaggtcg cgctcgagac gaggccgggc gcgttcgacg
     1441 gtttcccggg tctcggcgag gcttccatgg ccacgctcgt cagaaggtcg ctggccggca
     1501 gcgccggcct gtcgctgagc gacggctcga gcgaggtatc agtctcggcg acctatcccg
     1561 acggcctcct cgactcacgc gacgagctgg cccgcttcct cgtaccgtgg aagggcacgg
     1621 gcgtcccgct caagcacttc ttcgacttcg aggaatccga gaccgtgtcg caggtctact
     1681 ccgagaacgg cgaggccgcc tacaggctct acgccaacat ctcgggcgag gcgagcgacg
     1741 cggtgcggct cgagaaggaa gcggcggccc gccggctgtt cgactcggcc atcgagccgc
     1801 cggccggcta cggctattcc ttcgacaacc cgcgagggga gctcgacgag gccatctcgt
     1861 cactcctggc cgccctggcg atatccatcg ggctcgtgta cctgctcctg gccttccagt
     1921 acgactcgtt cggcgtgccg ttggctatcc tcgtagccgt gccgttcggc ttcgtcggcg
     1981 tggcgctgtc cttgttcgcg ttccgctcga cgctcaacct caattcgctg ctcggcacca
     2041 tcctgctctg cggcgtcgtc gtcaataact cgatcatcat gatcgacttc tacctgaaga
     2101 cgcgcaagaa ccatcaggac aacgcgagcg ccatcgaggc cgctgcggcc ctacggctca
     2161 agccgatact gatcacgacg ctgacgacgg tattcgggat gctaccgata gcgctcggca
     2221 tgggctccgg ctcgaacatc ctgcagccac tcggcatagc ggtctcgggc gggctggcga
     2281 tctctacgct cctgacgcta ttcgccgtgc cggcgctgct caggttctgg aggttccccg
     2341 cctaggccag gcggctactt cttgccgtag accgcctgag gctgggtctt gccgcggaac
     2401 aggacctcgc ccatcggctg gaagcgggac tgcggcttga gcgacaactg cttgtagacc
     2461 ggctggctga tgagcaggtc ctgcttgaac tcgcggcaca gcgagtcgag ccgggccgcg
     2521 atgttgaccg cctcgccgac cacgccgacg cgctgcacgc cgctcgccga cgatagctcg
     2581 ccgacggcca ccttgccggc gtgcacgcct atgccgatgt gcttgaccag gggcaggcga
     2641 tgggccgcca ggtcctcctg caggtgtgaa aactcataca ggaagtcgaa cgcgcagctg
     2701 acggcctggt cggcggcgtc cttctcgccc atcaggccga acacgacgat ggccgtgtcg
     2761 ccgacgaatt gctcgacgac gccgccgtgc ttgcgcgcga caatgtccca gagcgcgtag
     2821 tagcggttga ggaggcctat cgtcgccctg gcgccctcct tctcggctat cggggtaaaa
     2881 ttccagaggt cggcgtacag tatgaccacc tcgcgattct cgccgtcgga cacctggtcg
     2941 aacatcgacg gctcggccga aggcggctcc acgatcgagt acacctggtc aagcctggcc
     3001 ttcatcgaca cctgcttgat cttgtcggcc acctggaagg agccgaacat aaccgcgccg
     3061 aacatgaaga agacgtgctg cagggtcttg gcgaactcgc cgaaggccgg accgaagttc
     3121 gaaagtttga ggctcgagac caacgctgcg tagccgagtt cgccggcctc gtaggccttg
     3181 gtgctggtca ggcggaggtc caggtagaaa tagaagaaga tgaacgacag gatagtgccg
     3241 agcgatagga atatctcggc gatacgcttg ggccacttgc gccggaacag gatggccagc
     3301 gcctggatgc cgcccaggta tagagaggta gcccagagga agaagctggc catatcgatg
     3361 ccctgggccg acttcgacag cgtcaggatg gtcgtatagc cgagcggcgt catcagcgag
     3421 aagaagaacc tggccggcgg ccggtgaccg aagcgcgcca ggagcgctat ctgaaggagc
     3481 aggaacacga tcagcgatat gagcgccgag gtgctcggcc tgatgatggc cggcgaggtg
     3541 aacaggatga tgatgaagaa gagtacgaat tggctcgaat agaggaagaa ctcttctagg
     3601 taacgcttcg gtaggaatct catgtagtcg accatcatac ccgata
//
LOCUS       DAOOLN010000039         3602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_31297_length_3602_cov_2.919932, whole genome shotgun sequence.
ACCESSION   DAOOLN010000039 DAOOLN010000000
VERSION     DAOOLN010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31297_length_3602_cov_2.919932"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            309..1205
                     /locus_tag="PLW80_00855"
     CDS             309..1205
                     /locus_tag="PLW80_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HPB65078.1"
                     /translation="MNKIVSKARLSDEVFRIEVEAPNIARERKAGQFVIVQTDTEWGE
                     RVPLTIADADPQRGTVTLIFQTVGVSTHKLAELEPGQAIEAILGPLGNPTHIERFGRA
                     VCVGGGIGVAPMYPIAQALKRAGNRLTVIIGARTRDLVILEDEMRAIADELIVVTDDG
                     SHGRKALVTEPLKELCGSATPPDLVVAIGPPVMMKFCAEATRPFGVRTLASLNTIMID
                     GTGMCGGCRVSVGGQTRFVCVDGPEFDAHQVDWDGMLLRLRAYKSQEDAAHKALHESG
                     QCRLDAMAEAARTQAHAEGGSR"
     gene            1202..2692
                     /gene="gltA"
                     /locus_tag="PLW80_00860"
     CDS             1202..2692
                     /gene="gltA"
                     /locus_tag="PLW80_00860"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HPB65079.1"
                     /translation="MNLHNAGELGSKAKEVLAGLDGRELGNKDRMALPVQDMPCQDPL
                     DRAHNMEEVALGYTEAQARIEAARCLQCKNAPCVSGCPVSIDIPGFIAQVAKGDYHAA
                     ADVIRRTNLLPAICGRVCPQEKQCQAPCTLGKALKGVDKAVQIGRLERFVADRERAAG
                     GAPMPRVAAPTGKRVAVIGAGPAGLTVAADVRREGHEVVIFEAFQKPGGVMVYGIPEF
                     RLPKAIVREEAEKLERMGVSFELNYLVGRTRPLSALLEEDGFDAAFVGVGAGLPKFMG
                     IPGENLVGVFSANEYLTRANLMRAWDRGRAATPIYPSRRVAVLGGGNVAMDSARMALR
                     LGADEVHVLYRRTRDEMPARAEEVGHAIEEGIVFDFLASPTEVLGDDKGAVRGLRVMM
                     YELGEPDASGRRSPVAIKGSERDVPYDTVIVAVGNDSNPLLAKTTPGLAVDKKGHVLV
                     DENQKSSLDGVWAGGDIVLGAATVILAMGEGRRAAAAINEYLSRGE"
     gene            2797..3552
                     /locus_tag="PLW80_00865"
     CDS             2797..3552
                     /locus_tag="PLW80_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB65080.1"
                     /translation="MRFSVCTVTILSLVVALGSVVAVSAQEPSQAPAQPDLPAPAIAV
                     PAQAQPSLPVKADALLLYRQGRDLETAGKTAEAAAKYRESVAVCDRELATDPTRMDAY
                     TVKCWSLFRLERYREVVDIGAAALKVRFDARVVEVMGEAYFHLGDDKSALKYLQRYLD
                     NVGEYGDRVSTAYFYMAESYYRMKRFDHADIAYSMAVYREPGLARWWFRYASAVESLG
                     EYTRAFDLYGKALKLSPSMAEAQSGQSRVKARM"
BASE COUNT          625 a         1174 c         1264 g          539 t
ORIGIN      
        1 agggctatac agacgggcgc gacgaggcgc tgggcggagt ggcgtaccat gggcggtctc
       61 ccagtgatga attttctatc gattagatcg tcgatacgcg aaagaaccta tcacgagctg
      121 cctattaaac caagcgccgg agcgacgaat cgtcggtttg gttgtatcgt agtcttgata
      181 tcgatacaaa tatagttata caatgtggcg aaacgcgggc atgggccggc gtcaagacgt
      241 gaaacgctcg acgaggatag cgcgatgctc tattatggtg gcgtttccgg tagcgaagag
      301 gaggaagggt gaacaagata gtatcgaagg cccgcctgtc cgatgaggta ttccgcatcg
      361 aggtggaggc cccgaacata gcccgcgagc gcaaggccgg gcagttcgtg atcgtccaga
      421 ccgataccga gtggggcgag cgcgtcccct tgaccatagc cgacgccgac ccgcagcgcg
      481 gtaccgtcac gctgatcttc cagacggtcg gcgtcagcac gcacaagctc gccgagctcg
      541 aaccgggcca ggccatcgag gcgatactcg ggccgctggg gaacccgacg catatcgagc
      601 gcttcgggcg cgccgtctgc gtcggcggcg gcatcggcgt ggcgccgatg tacccgattg
      661 cccaggcgct gaagcgcgcc ggcaaccgcc tgacggtgat catcggcgcg aggacccgcg
      721 acctggtgat actcgaggac gagatgcgcg ccatcgccga cgagctgatc gtggtgaccg
      781 acgacggcag ccacggccgc aaggccctcg tcaccgagcc gctcaaggag ctgtgcgggt
      841 ccgctacccc gccggacctc gtcgtcgcga taggcccgcc ggtgatgatg aagttctgcg
      901 ccgaggcgac caggcccttc ggcgtgagga cgctcgcgtc gctcaacacg atcatgatcg
      961 acggcacggg catgtgcggc ggctgccgcg tctcggtcgg cggccagacc aggttcgtct
     1021 gcgtcgacgg gcccgagttc gacgcccacc aggtggactg ggacggcatg ctgctcaggc
     1081 tgcgcgccta taagtcgcag gaagacgcgg cgcacaaggc gctgcacgag agcggccagt
     1141 gcaggctcga cgcgatggcc gaggcggcca ggacccaggc ccacgccgag ggaggctcgc
     1201 gatgaacctg cataacgcgg gggagctcgg ctccaaggcc aaggaagtcc tggccggcct
     1261 cgacggccgc gagctcggga acaaggaccg gatggccctg cccgtgcagg atatgccctg
     1321 ccaggacccg ctggatcgcg cccataacat ggaagaggtg gcgctcggct acaccgaggc
     1381 ccaggccagg atcgaggccg ctcgctgcct gcagtgcaag aacgcgccct gcgtgtccgg
     1441 ttgcccggta tccatagata ttcccggctt catcgcgcag gtggccaagg gcgactacca
     1501 cgccgcggcc gatgttatac gcaggaccaa cctgctgccg gccatctgcg gccgcgtctg
     1561 ccctcaggag aagcagtgcc aggcgccctg caccctcggc aaggcgctca agggcgtcga
     1621 caaggccgtg cagattggtc gcctcgagcg cttcgtggcc gaccgggagc gcgcggcagg
     1681 aggggcgccg atgccccgcg tggccgcccc gaccggcaag cgggtggccg tgataggcgc
     1741 cggccccgcc ggcctgaccg tagcggccga cgtgcgccgc gagggccacg aggtggtcat
     1801 cttcgaggcc ttccagaagc caggcggcgt gatggtctac ggtatcccgg agttccgcct
     1861 gcccaaggcc atagtccgcg aggaggccga gaagctcgag cggatgggcg tgagcttcga
     1921 gctgaactac ctggtcggcc gcacgcggcc gctgtccgcc ctgctcgagg aggacggctt
     1981 cgacgcggcc ttcgtcggcg tcggcgccgg cctgccgaag ttcatgggca tacccggcga
     2041 gaacctcgtc ggcgtgttca gcgccaacga gtacctgacc cgcgccaacc taatgcgcgc
     2101 ctgggaccgc gggcgcgccg ccacgccgat ctacccgtcc aggcgggtcg cggtgctcgg
     2161 cggcggcaac gtcgcgatgg actcggcgcg catggccctg aggctcggcg ccgacgaggt
     2221 gcacgtattg taccggcgca cccgcgacga gatgccggcc cgcgccgagg aggtggggca
     2281 cgcgatcgag gaaggcatcg tcttcgactt cctggccagc cccaccgagg tgctcggcga
     2341 cgataagggc gcggtccggg gcctccgcgt gatgatgtac gagctcggcg agcccgacgc
     2401 gtccggtagg cgcagccccg tcgcgatcaa gggctcggag cgggacgtgc cctacgacac
     2461 ggtcatcgtc gccgtgggca acgactcgaa tccgctcctg gccaagacga cgccgggtct
     2521 ggcggtcgac aagaagggcc acgtgctggt cgacgagaac cagaagagct cgctcgacgg
     2581 cgtctgggcc ggaggcgaca tcgtgctcgg cgccgcgacc gtgatactcg cgatgggcga
     2641 gggacggcga gccgcggcgg cgattaacga gtacctgagc cgaggcgaat agggcgcggg
     2701 tcattgatcc gaagcccggc ttcggccata atgggggtcg agggcgcgtc gatcggcgtg
     2761 tccccgaccc ttttctgtcg tacggagagt cttggcatgc gatttagcgt ctgtaccgtc
     2821 actatcctca gcctcgtcgt cgccttgggt tccgtcgtgg ccgtatcggc ccaggaaccg
     2881 tcccaagcgc cggcccagcc ggacttgccg gctccggcga tagccgttcc cgcccaggcg
     2941 caaccctcat tgccggtcaa ggccgacgcc ttgctcctgt accggcaggg gcgagacctg
     3001 gagacggccg gcaagacggc cgaggccgcg gccaagtaca gggaatcggt agcggtctgc
     3061 gaccgggagc tggccaccga cccgacgagg atggacgcct acacggtcaa gtgctggagc
     3121 ctgttccggc tcgagcgcta ccgcgaggtg gtcgacatcg gcgccgccgc cctcaaggtg
     3181 cgcttcgacg cgcgcgtggt cgaggtgatg ggcgaggcgt acttccacct gggggacgat
     3241 aagagcgccc tcaagtacct gcagcgctat ctggacaacg tcggggagta cggcgaccgc
     3301 gtctccaccg cctacttcta tatggccgag agctactatc ggatgaaacg cttcgaccac
     3361 gccgacattg cgtacagcat ggccgtctac cgggagcccg gcctcgcgcg ttggtggttc
     3421 cgctacgcgt cggcggtcga gtcgctcggc gagtacacgc gcgccttcga cctctacggc
     3481 aaggccctca agcttagccc gagcatggcc gaggcgcaga gcggacagag ccgcgtcaag
     3541 gccaggatgt aggccgggcc gctaccccgc tagagcaggc tgaccgggtc cgggtctatc
     3601 tc
//
LOCUS       DAOOLN010000040         3580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_31562_length_3580_cov_3.142411, whole genome shotgun sequence.
ACCESSION   DAOOLN010000040 DAOOLN010000000
VERSION     DAOOLN010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31562_length_3580_cov_3.142411"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PLW80_00870"
     CDS             complement(<1..762)
                     /locus_tag="PLW80_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB65081.1"
                     /translation="MFDPPLAPANGVRVCVIGGGGTGAALAYDLCLRGFSVTLLEKGE
                     LTSGTTGRHHGQLHSGARYAVGDRAIARECMEETLILRRIAPDAVEYNGGVFVALSDD
                     EADYAPVFTAACVESGIPAREVTLAEARAWEPAINPAARRAVWVPDGAFDAFRVPLAF
                     FAAARRLGAVIRPFVEAVGFDLTGGRVRAVFARPRGSTRDERIECDYVVSATGAWAGA
                     VGRLAGLDVPITPAPGTMVAVRGRVSDMVVSRLAPP"
     gene            complement(852..1523)
                     /locus_tag="PLW80_00875"
     CDS             complement(852..1523)
                     /locus_tag="PLW80_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB65082.1"
                     /translation="MVDVLILDSEERIKAFTHPYRMRLVHVFRETRRPMTATEVARVI
                     GDGPGRVHYHMRVLEAAGIVTVARTETVNGIVARYYEPTARHYSVGEAARGAIGGEPM
                     RDEVTRMISRRFREGLRAFLDSATSGDADGVGSGEAEACPSAFLLERTVHCDDEAWLG
                     FRDAVEALIERYAEPAPGSKRRRVFMAGATDRPNTEREARPPRRRRAGPHDRPDWPPF
                     PELER"
     gene            complement(1676..2269)
                     /locus_tag="PLW80_00880"
     CDS             complement(1676..2269)
                     /locus_tag="PLW80_00880"
                     /inference="COORDINATES: protein motif:HMM:NF021612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase-like protein"
                     /protein_id="HPB65083.1"
                     /translation="MPLIDSIAQAEVRIGSWPAALILLAVVIVAVVALALWLGLAIGR
                     RSGRLEAERSGREAEAAARDDAVRRSRAVLTGQIGEQLAPYLPGFPCDPADARFIGKP
                     IDFIAFPGASAGAPVEALFIEVKTGDSRLSAAERALREAVEAGRVRWVEYRPPVGSRA
                     PGLSSAAGLAAADTRPGPGGGPSGSAVPTKAPRRPCA"
     gene            2259..3092
                     /locus_tag="PLW80_00885"
     CDS             2259..3092
                     /locus_tag="PLW80_00885"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HPB65084.1"
                     /translation="MSGILRLLKRVYGASVKRKAMRDADTSAGTAIDDALGRAAELVS
                     GSSYLIAFTGAGVSAESGIPTFRGPGGIWGSYDESHLELGFFMREPEIAWKTIKQIFY
                     SFTLSVEPNDAHRVLARWEAAGRLRLVVTQNIDGLHRRAGSRKVAEFHGSCDELVCMR
                     CGGRVPATAERLEALPPRCECGGLLKPGFVFFGEGIPEDAYETAFSAAALADACLVIG
                     STGTVYPAASIPRAVKRAGGRVVEINPEPSEFTDGVSDVFIRLPAAEAMRRLEAAVVR
                     L"
     gene            3089..>3580
                     /locus_tag="PLW80_00890"
     CDS             3089..>3580
                     /locus_tag="PLW80_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPB65085.1"
                     /translation="MSGPIPFSVVFLTAAAGPGTRAAPFGAARVASALKAWSAPQPAA
                     SAGSNDAPSGPGRPIHASIVEAEPDESPEALAARTLALSPDLVGLSLYSWNSVRLEAA
                     GAALKAARPGLVIVAGGPDASADPEALAARGPADVVVAGEGERVIVDLVAAMLSGKPL
                     PGPV"
BASE COUNT          536 a         1293 c         1251 g          500 t
ORIGIN      
        1 gggaggggcc agtcgtgata cgaccatatc ggagacgcgg ccgcgcaccg cgaccatcgt
       61 gccaggcgcg ggcgtgatcg gcacgtcgag gccggccagc cgcccgaccg ccccggccca
      121 ggcgccggtc gcgctgacga cgtagtcgca ctcgatcctc tcgtccctgg tcgagccgcg
      181 gggcctcgcg aagaccgcgc gcaccctgcc cccggtcagg tcgaagccga ccgcctcgac
      241 gaagggcctg atcacggcgc cgagtcggcg cgccgcggcg aagaaggcca acggcaccct
      301 gaaggcgtcg aaggcgccgt ccggcaccca gaccgcgcgc ctcgcggccg ggtttatggc
      361 cggctcccag gcgcgggcct cggcgagcgt cacctcgcgc gccgggatgc cggactcgac
      421 gcaggccgcc gtgaagaccg gcgcatagtc ggcttcgtcg tcgctcaggg ccacgaacac
      481 gccgccatta tactcgacgg cgtccggagc gatacgcctg aggatcagcg tctcctccat
      541 gcactcgcgg gctatagccc ggtcgccgac cgcgtagcgg gcgcccgaat gcagctggcc
      601 gtggtggcgg ccggtggtcc cggaagtcag ttcgcccttc tcgagcagcg tcaccgagaa
      661 gccgcggagg cacaggtcgt aggcgagcgc ggcaccggtg ccgcccccgc cgatcacgca
      721 gacgcggacg ccgttagcgg gcgccagggg tggatcgaac atgatggcga gtatactggc
      781 agtcggtgga gcgggcaagc gcgaccgaac cctcgatccg cgagcgcgac caataagatt
      841 cgtaacaccg ctcagcgttc gagctcggga aagggcggcc aatcgggccg atcgtgtgga
      901 ccggcccgtc ggcgacgcgg tggccgggcc tcacgctcgg tgttcggtct atcagtggcg
      961 ccggccatga acaccctccg gcgcttcgag ccgggcgcag gctcggcgta gcgctcgatc
     1021 agggcctcga ccgcgtctcg gaagccgagc caggcctcgt cgtcgcagtg gaccgttcgc
     1081 tcgaggagga aggccgacgg acaggcctct gcctccccgg aacccactcc atcggcgtct
     1141 ccggaggtcg cgctgtccag gaaggcgcgg agcccttcgc ggaagcggcg gctgatcatg
     1201 cgggtcactt cgtcgcgcat cggctccccg cctatcgcgc cgcgcgccgc ttccccgacg
     1261 ctatagtgcc gggcggtcgg ctcgtagtaa cgcgccacga tgccgttgac cgtctccgta
     1321 cgcgccaccg tcacgatacc ggcggcctcg agcacgcgca tgtggtagtg cacccgaccc
     1381 ggcccatccc caatgaccct cgccacctcg gtggccgtca tcggacgcct cgtctccctg
     1441 aacacatgca ccagcctcat ccgatacggg tgcgtgaagg ccttgatgcg ttcttcgctg
     1501 tcgaggatca ggacgtctac catgcgccca gcataacgtt cagctttatt gaacgtcaag
     1561 gaaagcgtac gatgcgggag gtgaggagcc ggatgctatc cataaggttc gcaacaccct
     1621 acacggctcg agtcacgagg cgctatgcct aaggttcata ccactaaggc ccgcgctagg
     1681 cgcacgggcg acgaggggcc ttcgtcggaa cggcggatcc tgacggcccg ccgcccgggc
     1741 ccgggcgcgt atcggcggcg gccaagcccg ccgcggacga gagccccggc gcccgggacc
     1801 cgaccggagg ccggtactcc acccagcgca cgcggccggc ctccacggcc tcccgtagcg
     1861 ctcgctcggc cgccgacaga cgcgagtcgc cggtcttcac ctcgatgaac agcgcctcga
     1921 cgggggcccc tgccgaggcg ccggggaagg cgatgaagtc tatcggcttg cctatgaagc
     1981 gggcgtcggc cgggtcgcag gggaagccgg gcaggtacgg ggccagctgc tcgcctatct
     2041 gcccggtcag taccgcgcgg gagcggcgca cggcgtcatc gcgggcggcc gcttcggcct
     2101 cgcggccgga acgctcggcc tcgagtcggc ccgatcggcg cccgatggcc aggcctagcc
     2161 acagcgcgag cgccaccacg gctacgatga cgacggcgag caggatgagc gcggctggcc
     2221 aggagcctat cctgacctcc gcttgcgcta tggagtcgat gagcggcata ctgaggctac
     2281 tgaagcgcgt ttacggcgcc tcggtcaaga ggaaagccat gcgagacgcg gataccagcg
     2341 ccgggacggc gatcgacgac gctctagggc gcgcggccga gcttgtctcg ggctcgagct
     2401 acctgatagc ctttaccggg gccggcgtgt ctgccgagtc gggcataccc accttccgtg
     2461 gcccgggcgg catctgggga tcctacgacg agagccacct ggagcttggc ttcttcatgc
     2521 gtgagccgga gatagcctgg aagacgatca agcagatctt ctattccttc accctaagcg
     2581 tcgagccgaa cgacgcgcat cgcgtcctgg cccgctggga ggccgccggc aggctcaggc
     2641 tcgtcgtcac gcagaacata gacgggctgc accgccgcgc cggctcgagg aaggtggccg
     2701 agttccacgg ttcctgcgac gagctggtct gcatgcgctg cggcggccgc gtcccggcga
     2761 ccgccgagcg actcgaagcc ttgccgccgc gctgcgagtg cggcgggctc ctgaagccgg
     2821 gtttcgtgtt cttcggcgag ggcataccgg aggacgccta cgagacggcc ttctccgcgg
     2881 ccgcgctcgc ggacgcctgc ctcgtgatcg gctcgaccgg caccgtctac ccggcggcct
     2941 cgataccgcg cgcggtcaag cgagcgggcg gccgcgtcgt cgagatcaac cccgagccga
     3001 gcgagttcac cgacggggtc agcgacgtgt tcatccgcct gccggccgcc gaggcgatgc
     3061 gcaggctcga ggccgcggtg gtaaggctat gagcggcccg ataccgttct cggtcgtctt
     3121 cctgacggcg gccgcggggc cgggcacgcg ggccgcgccg ttcggtgccg cccgcgtcgc
     3181 gtcggcgctc aaggcctggt ccgcgccaca gcccgcggcg agcgccggat cgaacgacgc
     3241 cccatccggc cccggccgtc cgatacacgc gtccatcgtc gaggccgagc ccgacgagtc
     3301 gcccgaggcc ctcgcggccc ggacgctcgc cctctcgccc gacctcgtcg ggctgtcgct
     3361 gtactcgtgg aactcggtcc ggctcgaggc cgccggggcg gctctcaagg ccgctcgccc
     3421 cggcctcgtc atcgtcgccg gagggccgga cgccagcgcc gatcccgagg cgctcgcggc
     3481 gcgcggcccg gccgacgtcg tggtggccgg cgaaggcgaa cgggtcatcg tcgacctcgt
     3541 cgccgccatg ctctccggaa agccactgcc gggacccgtc
//
LOCUS       DAOOLN010000041         3525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_32206_length_3525_cov_2.998271, whole genome shotgun sequence.
ACCESSION   DAOOLN010000041 DAOOLN010000000
VERSION     DAOOLN010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32206_length_3525_cov_2.998271"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PLW80_00895"
     CDS             <1..635
                     /locus_tag="PLW80_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPB65086.1"
                     /translation="HANSDDHAQSHRDGDRFESFAKAMAELGETGVSLFSFHSTSKGY
                     LGECGQRGGYMEVRNVGPEVMAQVLKMQSVSLCANLPGQVAMYSLVAPPAVGGPSRPE
                     YDRERSAILGELRERARLLADGLNAIPGFHCNAVAGAMYAFPRLELPAGRTDEDWCMA
                     LLESKGICVVPGSGFGQLPGTAHFRTTILPPRDQLAEVVAAIASFNAEYR"
     gene            645..923
                     /locus_tag="PLW80_00900"
     CDS             645..923
                     /locus_tag="PLW80_00900"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPB65087.1"
                     /translation="MEWRASHILVSDRALAQELVRKAKQGADFGSLARQFSTCPSKSS
                     GGDLGWFGPGKMVAPFEHACKGLGVGSISDVVQTQFGYHVIKVTGKRD"
     gene            1000..2469
                     /locus_tag="PLW80_00905"
     CDS             1000..2469
                     /locus_tag="PLW80_00905"
                     /inference="COORDINATES: protein motif:HMM:NF039513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5716 family protein"
                     /protein_id="HPB65088.1"
                     /translation="MGIRSPGVFATLPQLFFSPLASPNREHYAALLVIYYRLFQESPH
                     GIERKALLYRFAEYIEQHRSQFEAEEGIGDAPDSPVDERTSAETGESLFPPEETSAVE
                     PARYLAARAIRTFIAAGWMSEEVLPDYTRILNMVSHARPFLEALARIEEGLKVEYESH
                     VVSVYSLLCGDAAKDNGHYLVLNAHGQTIALIDSLKVLSQSIREHYERLTGRSEGLDV
                     ADILALHYGEYASDILDGAYKRLKTSDNLSRYRPRILSQVRDFLLDRTWLDECAFKYS
                     RTASLPVSEARAKLVAMLEEIRDTLKAVDPLLEEIDRRNMLYAKSSVDRIKALLEPAS
                     SIAGRIMRAAQSICDTPQLYRELPYHLYRIQALTPESRYKRWLREILEFEYAEPTVAD
                     PAQFDRLEAEFRLRLERQLSPARISEWLDSKGGQSSPLVAAELAVTVDDFVRLVYAIL
                     FADSRPDSFSYFVEEIEGGKLVEVPAGWLVPDVRLRRSI"
     gene            2466..3083
                     /locus_tag="PLW80_00910"
     CDS             2466..3083
                     /locus_tag="PLW80_00910"
                     /inference="COORDINATES: protein motif:HMM:NF025205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HPB65089.1"
                     /translation="MTIDTAYLSMIGDLTDGETDLFRLAAQTLMSRSFIVRGTEKADV
                     LWDFSIRNIHLLEAWFACAGIQLKRDEMLGVIALRPGASMRARLGKEETCALLVARLS
                     FEEKRVELSLSRFPSIRILDFVQRYRTVIGIDLKKTRLAEILRSFSRWRLIGVEGDPT
                     DPESAIVLYPSLALTLDQAAVDEVLQAVTSVAPETDPSEEDEDVE"
     gene            3080..>3525
                     /locus_tag="PLW80_00915"
     CDS             3080..>3525
                     /locus_tag="PLW80_00915"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB65090.1"
                     /translation="MITLRWVRLVNWHYFRDETLRIGERSLISGDNGMGKSTIVDAIQ
                     YALAADLRKARFNQAAGDRRGGRDLVGYVRCKIGSDTTEYLRGDTVSHVMLQFEANDE
                     AFTAAVCVEAFSDGRCTERFWISQGIGVDSISVHDGKNKPLTWRQF"
BASE COUNT          690 a         1157 c         1044 g          634 t
ORIGIN      
        1 atcacgccaa ctccgacgat cacgcccagt ctcaccgcga cggcgaccgc ttcgagtcgt
       61 tcgccaaggc gatggccgaa ctcggcgaga ccggcgtgtc gctgttcagc ttccactcga
      121 ccagcaaggg atacttaggc gagtgcggcc agagaggcgg ctacatggag gtccgcaacg
      181 tcgggcccga ggtgatggcc caggtgctca agatgcagtc cgtcagcctc tgcgccaacc
      241 tccccggcca ggtggccatg tacagcctcg tcgccccgcc cgcggtcggc ggaccgagcc
      301 gccccgagta cgaccgcgag cgctcggcca ttctcggcga gctgcgcgag cgcgcgaggc
      361 tcctcgccga cggcctcaac gccatccccg gcttccactg caacgccgtc gccggcgcca
      421 tgtacgcctt cccccgcctc gagctaccgg cgggcaggac cgacgaggac tggtgcatgg
      481 ccctgctcga gagcaagggc atctgcgtcg tgccgggctc gggcttcgga cagctacccg
      541 gtaccgccca cttccgcacc accatactgc cgccccgaga ccagctcgcc gaggtcgtgg
      601 cggcgatagc gtcgttcaac gccgagtacc gataggagac gatcatggaa tggagagcca
      661 gccacatact cgtcagcgac cgcgccctgg cgcaggagct cgtacgcaag gccaagcagg
      721 gagccgactt cggctcgctc gcgaggcagt tctcgacctg cccgtccaag tcgtcgggag
      781 gcgacctggg ctggttcggc cccggcaaga tggtcgcccc cttcgagcac gcctgcaagg
      841 gcctcggcgt cggctccatc tccgacgtcg tccagaccca gttcggctac cacgtcatca
      901 aggtgaccgg gaagcgggac tagagttatg aaggccgcgc gaccacgacc tcgaggagac
      961 gcgatggcca tgatgccatg cctgatacta taatcaggca tgggcatccg ttccccgggg
     1021 gttttcgcaa ccctcccgca gttattcttc tctccgcttg ccagccctaa ccgggagcac
     1081 tacgccgccc tactcgtcat ctactaccga ctgttccagg agtcgccgca cgggatcgag
     1141 cgcaaggccc tgctatatcg attcgccgaa tacatcgagc agcatcgtag tcagttcgag
     1201 gcggaagagg gcatcggcga cgcgccggat tcccccgtcg atgaacggac aagcgctgaa
     1261 accggggaga gcctcttccc acccgaggag acgagcgcgg ttgagccggc gcgttactta
     1321 gccgcgcgcg ctatacgcac cttcatcgcc gccggttgga tgagcgaaga agtcctaccc
     1381 gactatacgc gaatcctaaa catggtctcc cacgcccgcc cgttcctcga ggccctcgcc
     1441 cgtatcgaag agggcctcaa agtggagtac gagagccatg tcgtctcggt ctattcgcta
     1501 ctctgcggag acgcggccaa ggataacggg cactacctcg tgttgaacgc ccacggccag
     1561 accattgccc tcatcgattc gctcaaggtc ctctcacaga gcatccgcga gcattatgag
     1621 cgccttacgg gtaggagcga aggactcgat gtggcggaca tcctcgcgct tcactacggc
     1681 gaatatgcct cggacatact cgacggcgct tacaagcggc ttaagacctc ggataaccta
     1741 tcgcgctata ggccgcgcat actctcgcag gtacgggact tcctgctcga ccggacttgg
     1801 ctcgacgaat gcgccttcaa gtattcgcgt acggcctcgc tccccgtctc cgaagcgcgc
     1861 gctaagctcg tagcaatgtt ggaggagatc cgggacaccc taaaggcagt cgaccccctg
     1921 ctagaggaga tagataggcg caacatgctc tacgctaagt caagcgtcga tcggataaag
     1981 gccctgctag aaccggcgtc ctccatcgca ggccgcatca tgcgcgccgc gcaatccatt
     2041 tgcgataccc cgcagctcta ccgggaacta ccgtatcacc tctaccgtat ccaggcgctg
     2101 accccggaat cgcgatacaa gcgctggcta cgcgagatcc tggaatttga atacgccgaa
     2161 ccgaccgtag cagatccggc ccaattcgat cgtctcgaag ctgagttcag gctacgactt
     2221 gaacgccagt tgagccccgc acgtatctcg gagtggctcg actccaaggg gggacagagc
     2281 tcgcctttgg tcgccgccga gctcgccgtc accgtcgatg atttcgtaag gctcgtgtac
     2341 gctattctct tcgccgactc gcgccctgat tctttctcgt acttcgtaga ggagatcgaa
     2401 ggcggaaagc tcgtagaagt gcccgcgggg tggctcgtac cggacgtacg cctgaggaga
     2461 agtatatgac catcgatacc gcttacctca gtatgatagg agacctaacc gacggcgaga
     2521 cggacctctt ccgtctcgcc gcccagaccc tgatgtcccg tagtttcatc gtgcgcggca
     2581 cggagaaagc tgatgttctt tgggatttct cgatccggaa catacacctg ctcgaagcct
     2641 ggttcgcctg cgcgggtatc cagcttaagc gagacgagat gctcggcgtg atcgccctgc
     2701 gacccggcgc gtcgatgcgc gcgcgactcg ggaaggagga gacttgcgcc ctcttagtcg
     2761 cgcgtctctc gttcgaggag aagcgggtag aactgagcct ttcgcgcttt ccgtctatca
     2821 ggatattaga cttcgtgcaa cgctaccgca ctgtgatcgg aatcgacctc aagaagaccc
     2881 gcttggccga gatcctgcgc tctttcagcc gctggcggct tatcggcgta gaaggggacc
     2941 cgaccgatcc ggaaagcgcc atcgtcctct atccgtcgct tgccctcact ctcgaccaag
     3001 ccgccgtgga cgaggtactg caagcggtca cgtcagtagc gcccgagacg gatccctcgg
     3061 aggaagatga ggacgtggaa tgataacgct acgctgggtt cgcctcgtga actggcatta
     3121 cttccgcgac gagacgctgc gtatcggcga acgatcgctt atttccggag acaacggcat
     3181 gggaaagtcc acgatagtgg acgccatcca atacgccctc gcggccgacc tgcgcaaggc
     3241 ccgcttcaac caagcggcgg gggacaggcg cggcggacgc gacctcgtcg gatacgtgcg
     3301 ctgcaagatc ggtagcgata ccaccgaata cctgcgcggc gataccgtct ctcatgtgat
     3361 gctccagttc gaggcgaacg acgaggcgtt caccgccgcc gtctgcgtcg aggccttttc
     3421 ggacgggcgc tgcacggagc ggttctggat aagccagggt atcggcgtag attccatctc
     3481 cgtgcacgac gggaagaaca agcccttgac ctggcgccag ttcaa
//
LOCUS       DAOOLN010000042         3524 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_32219_length_3524_cov_3.349668, whole genome shotgun sequence.
ACCESSION   DAOOLN010000042 DAOOLN010000000
VERSION     DAOOLN010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32219_length_3524_cov_3.349668"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PLW80_00920"
     CDS             complement(<1..650)
                     /locus_tag="PLW80_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB65091.1"
                     /translation="MLRLFKYLKPYALMVALALLMEFASSMAELSLPALMSVIVDEGV
                     ATGDIALIWRTGARMLAIAFIGVAANLTGGFFAARSAMGFGKTLRSKVFSRVSSFSLR
                     ELDRFGTSSLITRSTNDITQVQQVTFMMQRMMAKAPIMAIGGIAMALATEPRLAWILL
                     VVIPILGAAIVIIALKGLPLFKSIQAKIDALNRVMRENLSGIRVVRAFDWVEHERDR"
     gene            complement(654..1154)
                     /locus_tag="PLW80_00925"
     CDS             complement(654..1154)
                     /locus_tag="PLW80_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HPB65092.1"
                     /translation="MIDDMRDERPIAERLFEAFRAIGPFKRRPSLGHAQRPGEFQVMH
                     RLSHEPPESGMRVGDLAAWLSVKPPTVTKAVDALVARGLVERYADEADRRVIRVRLSP
                     DGRTLDSSFREQALGEIRGLVDYLGEDDSATLARLLTRAAEYMNERYGRACNGACGRA
                     KERGAD"
     gene            1291..3153
                     /locus_tag="PLW80_00930"
     CDS             1291..3153
                     /locus_tag="PLW80_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HPB65093.1"
                     /translation="MSFLPTTRAELAARRLDGLDFVIVTADAYVDHPSFGAALIGRRL
                     ERLGYSVGVIARPDPTDVEAFRALGRPSLAFLVAGGAIDSMVCKYTANRKPRSTDEYA
                     PGGDPSLCRGADGSIRAFAVNGRPASARPDRAVIAFAGKCREAYKGVPVIAGGLEASL
                     RRLSHYDYLSDTVRRPIIVDSKADLIVYGMGERAIAEAARVLAEARSRSDSFDPAALR
                     GIRGTVWRCSRREDVPEGAVSLPDHEAAAADGRAFAESFAAQYRNTDPVSARPLVEAA
                     AGQFVVQEPPQLPLSRAELDELYGLGFERAWHPAYDRYGGVPALAEVRFSIASSRGCY
                     GACAFCALAFHQGRVVTSRSKESIVDEARLLTGLTGFKGYIHDVGGPTANFRGPACDK
                     QAGGGACVDRRCLAPEPCPALKADHRDYVSVLRAVRAVPGVKKAFVRSGVRFDYVMAD
                     SDRTFLRELAAHHVSGQLKVAPEHVSDRVLRLMGKPPRRVYDAFAGEWSRVNAELGLK
                     QFLVPYYIASHPGAELSDAIELAEYLRDSGFVPDQVQDFYPTPGTLATAMYRSGLDPL
                     TMVPVYVARGAKERSYQRALLQYSKPENRKLVIEALRLAGRTDLIGRGPKCLVS"
     gene            3260..>3524
                     /locus_tag="PLW80_00935"
     CDS             3260..>3524
                     /locus_tag="PLW80_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65094.1"
                     /translation="MTKRMRVALAPVLAIILALSACVGAPEPAPGVLDNVSTLPGGSA
                     YRPSDPLPLAPELTKRVLPNGLTYYVRRNGNPGGRAVMFLIVNS"
BASE COUNT          544 a         1235 c         1150 g          595 t
ORIGIN      
        1 cggtcccgct cgtgctcgac ccagtcgaag gcgcgcacca cccggatgcc ggagaggttc
       61 tcgcgcatca cgcggttgag cgcgtcgatc ttggcctgga tggatttgaa taggggtaga
      121 cccttgagcg cgatgatgac gatggccgcg ccgagtatcg ggatgacgac gagcaggatc
      181 cacgccaggc gcggttcggt agccagcgcc atcgcgatgc cgccgatggc catgatcggc
      241 gccttggcca tcatccgctg catcatgaag gtaacctgct ggacctgggt gatgtcgttc
      301 gtcgacctgg tgatcagcga cgaggtgccg aagcggtcga gttcccggag cgagaacgac
      361 gatacgcgcg agaagacctt ggagcgcagc gtcttcccga agcccatcgc cgacctggcc
      421 gcgaagaagc cgccggtcag gttggccgct acgcctatga acgctatggc cagcatccgg
      481 gcgccggtgc gccagatcag cgcgatatcg ccggtagcca cgccctcgtc cacgatgacc
      541 gacatcagcg ccggcaagct cagctccgcc atcgagctgg cgaattccat cagaagcgcc
      601 agcgctacca tgagcgcgta cggcttcagg tacttgaaga gtctcagcat gtcttaatcc
      661 gctcctcgtt ccttggctcg gccgcaggct ccattacagg cgcggccgta ccgttcgttc
      721 atgtactcgg cggcccgagt caggagccgc gcgagcgtcg cgctgtcatc ctcgcccagg
      781 tagtcgacga ggccccgtat ctcgccgagc gcctgctcgc ggaatgagct gtccagcgtt
      841 ctaccatcgg gagacaggcg caccctgatg acccgcctat ccgcctcgtc ggcgtagcgc
      901 tcgaccaggc ccctggcgac cagcgcgtcg accgccttgg tcacggtcgg cggcttgacc
      961 gacagccagg ccgccaggtc gccgacgcgc atgcccgact cggggggctc gtgcgacagt
     1021 cggtgcatga cctggaattc gcccggccgt tgggcatgcc cgaggctcgg ccggcgtttg
     1081 aacggtccga tggccctgaa cgcctcgaag agccgctcgg cgatcggccg ctcgtcccgc
     1141 atatcgtcga tcatgttcgt cctcgtagct tcgcatcctc atgcaattag ccttgctaaa
     1201 agttagcctt gctaaatata ttaacgccgg cttgcctggt caaggctatg accacgcgac
     1261 caagaaggcc cgggagagta tactacgggc atgtcgttcc tccccaccac gcgagccgaa
     1321 ctggccgcca ggcgcctcga cgggctcgat ttcgtcatcg tcacggcgga tgcctacgtc
     1381 gaccatccgt cgttcggcgc ggccctgatc gggcgcaggc tcgagcgcct cggctactcg
     1441 gtcggcgtca tagccaggcc cgacccgact gacgtcgagg ctttccgcgc cctcggtcgt
     1501 ccgtccctgg ccttcctcgt cgctggcggc gcgattgact cgatggtctg caagtacacc
     1561 gccaaccgca agccgcgctc gaccgacgag tacgcccctg gcggcgaccc gagcctctgc
     1621 cgcggcgccg acggctcgat acgggctttc gcggtcaacg gccggcctgc gagcgccagg
     1681 ccggaccggg cggtgatcgc cttcgccggc aagtgccgcg aggcctacaa gggcgtgccg
     1741 gtgatagccg gcggcctcga ggcctcgctg cgccgcctgt cgcactacga ctacctgtcg
     1801 gatacggtgc gcaggcccat catcgtcgac tccaaggccg acctcatcgt ctacggtatg
     1861 ggagagcgcg cgatcgccga ggcggcccgc gtcctcgccg aggcccgctc ccgatccgac
     1921 tcgttcgatc cggcggcgct acgaggcatc cgcggtacgg tatggcgctg ctcgcgccgg
     1981 gaagacgtgc cggagggcgc cgtgagccta cccgaccacg aggccgccgc cgcggacggc
     2041 cgggccttcg ccgagtcgtt cgcggcgcag taccgtaaca ccgacccggt atcggcccgg
     2101 cccctcgtcg aggcggccgc cggccagttc gtcgtgcagg agccgcctca gcttccgctg
     2161 tcccgcgcgg agctggacga gctatacggc ctcggcttcg agcgcgcttg gcacccggcc
     2221 tacgaccggt acgggggcgt accggccttg gccgaggtgc gcttcagcat agcctcgagc
     2281 cgaggctgct acggcgcttg cgccttctgc gctctcgcgt tccatcaggg ccgcgtcgta
     2341 acgagccggt caaaggagtc catcgtcgac gaggcgcggc tcttgacggg cttgaccggc
     2401 ttcaagggtt atatccatga tgtcggcggg ccgacagcca acttccgagg gcccgcctgc
     2461 gataagcagg ccgggggcgg cgcctgcgtc gatcggcgct gcctggctcc cgagccatgt
     2521 ccggcgctca aggccgacca ccgggactat gtctccgtgc tgcgggcggt ccgcgccgta
     2581 cccggcgtca agaaggcctt cgtgcgctcg ggcgtgcgct tcgactacgt gatggccgac
     2641 agcgaccgca ccttcctgcg cgaactggcc gctcaccacg tctccggcca gctcaaggtg
     2701 gcgccggagc acgtgtccga ccgggtgctc cgcctgatgg gaaagcctcc gcgccgcgtc
     2761 tacgacgctt tcgccggcga gtggtcgcgg gtgaacgccg agctcggtct aaagcagttc
     2821 ctggtgccgt actatatcgc ctcgcaccct ggcgctgagc tgtccgatgc gatagagctg
     2881 gccgagtacc tgcgcgactc gggcttcgtg cccgatcagg tgcaggactt ctacccgacg
     2941 ccgggtacgc tcgcgacggc catgtaccgg agcggcctcg acccgctgac gatggtgccg
     3001 gtctacgtgg cgcgcggcgc caaggagcga tcctaccagc gcgccctgct acagtactcg
     3061 aagcccgaga accgcaagct ggtcatcgag gcgctcaggc tggccggccg gaccgacctg
     3121 atagggcgcg ggccgaaatg cctcgtgtcg taacattggt cgtcggcgtg cctttacgct
     3181 atattcaaac gacggcctcg ccccgatggc tcggcactaa ggctcggcca ttaggctggc
     3241 gcgtcgataa ggagtaccca tgacgaagcg gatgcgggtc gccctggccc ccgttctagc
     3301 gatcatactg gccctgtcgg cctgcgtcgg cgccccggag ccagctcccg gcgtcctgga
     3361 caatgtatcg accctgcccg gcggcagcgc ctaccggccg tccgacccgt tgccgctggc
     3421 gcccgagctg accaagcgcg tgctgccgaa cggcctgacc tactatgtcc gacgtaacgg
     3481 caaccccggt ggccgagcgg tcatgttcct gatcgtcaac tcgg
//
LOCUS       DAOOLN010000043         3507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_32417_length_3507_cov_2.785342, whole genome shotgun sequence.
ACCESSION   DAOOLN010000043 DAOOLN010000000
VERSION     DAOOLN010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32417_length_3507_cov_2.785342"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3083
                     /locus_tag="PLW80_00940"
     CDS             <1..3083
                     /locus_tag="PLW80_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPB65095.1"
                     /translation="SGMKRVITSCAGRPVAVAMGLVAIMMAGIGAAIDLPRRSLPDVS
                     VPRVLVEATMPGMSAAAMRSLVTCPLEDALASADGLIGSSSVSRDGRTVITLDFNWGE
                     DAARMASNVRDIIDATYPGLPEGATKPSVTADDPAASPLVTVTMAPMAGDLAFAKRMA
                     EREGRARLRRVVGVGAVTVVGGRDRELAVAVDERKAAARGMTARDVASALAAECADVP
                     LGSVEGGGLELVVIAEGRPKTVAELEAVVAVSPAGSFRLSELARVYERDAPADSLYVL
                     DGIETVALEVYLRPGADPVATAREVVLVAEAMAVEFTDELAISTVGDSSTAVAASIRE
                     LAWAAVIGSIAAAVVLYAMLRDARCAVMVAVSIPICIAATLAALSLAGRSLNRMSLSG
                     LALAVGIISDNAVVTLDALSKVGETMPRGTSRRMYPTPAEIGEAASSVVGGTFGGMVT
                     TVAVFMPIFYLPGAIGSIFGDLAFAVVVASACGWVCAMTILPAAYRLFGTAGSGGRPP
                     ARLAASYARSLSTAMRRPSRLVICAAIAAAIGVALAATRDVSFMPRGAVSELLVTARF
                     EPGSDAEGIRPEAISLSAILSRVDGVVSTRGWAGAEVGDLSRLADPAYGPERLSLLCA
                     LSSKADTETIAREIAAAARAALPPSASLTVAEPPDPAAAIMGLVEGSTIVVNADSVEA
                     AKAAADAFESYLSDLAGDALASVVRSPDGQARRVLASPDRQRIAGLRVKLAEVEAVIR
                     TATEGVRVGSMRSDGEDTDIVVFAGGAGSADRGGSLESLRSITVEAGDSAPIHASTVA
                     TFDLAYSAATLARLDRRDAIYLTPVAALSGRGALEAAVERAMIAYGSGARSSVHRSGG
                     SAFGRYAEAMAWTLALVLVLLYLVIGSQFESFVAPLVIMAAIPLALAGSGPTMLIAGV
                     GLDSGSIMGLVALFGTVVNSAILLYEACAARLAAGEAPDSAAFLGAIDRLRPVLATTM
                     TTLVALVPLCLNPEASPQRSMAVSMIGGMAASTALTLFITPTVLAALARRRAAA"
     gene            3080..>3507
                     /locus_tag="PLW80_00945"
     CDS             3080..>3507
                     /locus_tag="PLW80_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPB65096.1"
                     /translation="MSDSRAHWYGKPVAAVCVAAAAVALSARTLAVTEFGGSPGSGGS
                     VCVVSLSQYGTNAREMESLVAIPLEDMLASLPGVIGTSSSCEYGEARVVARFAEGRDG
                     TEAVSAAAQRLYESLPRSVQRPRISSARDDVGPAWVAAAVS"
BASE COUNT          505 a         1165 c         1237 g          600 t
ORIGIN      
        1 atagcgggat gaagcgcgtc atcacgtcgt gcgcgggccg gccggtggcc gtggcgatgg
       61 gcttggtcgc catcatgatg gccggcatcg gggccgcgat agacctgccc aggcggagcc
      121 tgcccgatgt gtcggtacca cgcgtcctcg tcgaggcgac gatgcccggt atgagcgcgg
      181 cggcgatgcg ctccctcgtc acctgcccgc tcgaggacgc gctcgcctcg gcggacgggc
      241 tcatcgggtc gtcgagcgtt agccgggacg gacgtaccgt catcaccctc gatttcaact
      301 ggggcgagga cgcagcgagg atggcgtcga acgtacgcga tatcatcgat gcgacctatc
      361 cggggttgcc ggaaggcgcg accaagccaa gcgttactgc cgatgacccg gcggcgtcgc
      421 cgctcgtcac ggtgactatg gccccgatgg ccggggacct ggccttcgcc aagcgcatgg
      481 ccgaacgcga gggccgggcg aggttgcggc gcgtggtagg cgtcggcgcc gtgaccgtgg
      541 tcggcggcag ggatcgcgag ctagccgtcg cggtggacga gcgtaaagcg gccgcgcggg
      601 gcatgacggc aagggacgtg gcctcggcgc tggccgccga gtgcgccgac gtcccgctcg
      661 gttcggtcga gggcggagga ctcgagctcg tcgtcatagc ggagggtcgg ccgaagaccg
      721 tcgccgagct tgaagccgtg gtagccgtct ctcccgccgg ctcttttcgc ctatccgagc
      781 tggcacgggt ctatgagcga gacgcgccgg ccgatagtct ctacgtgctc gacggcatcg
      841 agacggtggc gctcgaggtc taccttcgtc caggcgccga cccggtggct acggctcgcg
      901 aggtggtact ggtggccgag gcgatggcgg tcgaattcac ggacgagctg gccattagta
      961 cggtcggtga ctcgtcgacg gccgtagcgg cctcgatacg ggaactggcc tgggcggccg
     1021 tcatcggctc gatcgcggcc gccgtcgtgc tgtacgccat gctccgggac gcccgctgcg
     1081 ccgtcatggt cgccgtttca atcccgatat gcatcgccgc aaccctggcc gctttgtcgc
     1141 tggcgggccg atcgctcaac cgcatgagcc tgtccggcct cgccctggcc gtgggtatca
     1201 tatcggacaa cgcggtggtt accctggatg ccctgtcaaa ggtgggagag acgatgccgc
     1261 gcggcaccag tcggcgaatg tacccgactc cggccgaaat tggcgaggcc gcttcctcgg
     1321 tagtcggcgg aacgttcggc ggcatggtaa cgaccgtggc cgtgttcatg cccatcttct
     1381 acttgccggg cgctatcggc tccattttcg gcgacctggc cttcgccgtc gtcgtcgcca
     1441 gcgcctgcgg ctgggtctgc gcaatgacca tactaccggc ggcgtatagg ctcttcggaa
     1501 cggccggctc gggggggcgg ccgccggcca ggctcgcggc atcgtacgcg cgctcgctgt
     1561 caacggctat gcgcaggccg tcgaggctcg tcatctgcgc cgctatcgca gccgcgatcg
     1621 gagtcgcctt ggcggccacg agagacgtat cgttcatgcc gcgcggagcc gtctccgaac
     1681 ttctcgtgac cgctcgattc gagcccggta gcgacgccga aggcatccgg ccggaggcaa
     1741 tatcgttaag cgccatccta tcgagggtcg acggcgtcgt gtcgacgagg ggttgggccg
     1801 gagccgaggt cggcgacctg tcgaggctcg ccgatcccgc gtatgggccc gagcgcctga
     1861 gcctcctctg cgccctatcc tcgaaggcgg acaccgaaac gatagccagg gagatagcgg
     1921 ccgccgcgag ggctgccctg cctccttcag cctcgctaac cgtggcggag cctccggatc
     1981 cggcggccgc catcatgggg ctcgtcgagg gttcgaccat cgtcgtgaac gccgacagcg
     2041 tcgaggcggc caaggcggcc gcggacgcgt tcgagtccta cctgtccgac ctggccggag
     2101 acgcgctcgc ttcggtcgta cggtcgccag atggccaggc cagacgagtc ctggccagcc
     2161 ctgataggca acggatagcc ggccttcgcg tcaagcttgc ggaggtcgaa gccgtcatca
     2221 ggaccgctac cgagggcgtg cgggttggga gcatgcgttc cgacggcgag gatacggata
     2281 tcgtcgtctt cgcaggcgga gccggcagcg cggatcgcgg agggagcctc gagtcactgc
     2341 gctcaatcac cgtcgaagcc ggggactccg cgcccataca cgcctcgacc gtcgcgacct
     2401 tcgacctggc ctacagcgcc gccacgctcg ccaggctgga ccggcgggac gcgatctact
     2461 tgacgccggt cgccgccctg tccggtcgcg gcgcgctcga agcggccgtc gagagggcca
     2521 tgatcgcgta cggcagcggc gccaggtcca gtgtccatcg ttccggcggc tccgccttcg
     2581 gtcgctacgc cgaggctatg gcatggacgc tcgcccttgt gctggtcctt ctatacttgg
     2641 tgatcggtag ccagttcgag tctttcgtgg cgccgttggt catcatggcc gccattcctt
     2701 tggccctggc cggatcgggt ccgacgatgc tgatcgccgg cgtcggcctc gactcggggt
     2761 cgatcatggg cctcgtcgcc ctgttcggca cggtcgtcaa tagcgcgata ctgctctacg
     2821 aggcctgcgc ggcacggctc gcggccggcg aggcgcccga tagcgccgcg ttcctagggg
     2881 ccatagacag gttgaggccg gtactggcga cgacgatgac gaccctggtc gccctcgttc
     2941 cgctctgcct gaatccagag gcgtcgcccc agcgttccat ggccgtgtcg atgataggcg
     3001 gcatggcggc gtccaccgcg cttaccctgt tcataacgcc gaccgtcctg gccgccctgg
     3061 ctcgacgaag ggccgcggca tgagcgattc ccgcgcccac tggtacggaa agccggtagc
     3121 ggcggtctgc gtcgccgccg ccgccgtagc cttgtcggct aggacgctcg ccgtgaccga
     3181 attcggaggc tcgcccggct cgggtggaag cgtctgtgtc gttagcttga gccagtacgg
     3241 cacgaacgcc cgcgagatgg agagcctcgt cgctataccg ctcgaggaca tgttggcctc
     3301 gctaccgggt gtcatcggca cgtcgagttc gtgcgaatac ggcgaagcca gggtggtagc
     3361 gcgcttcgcc gaaggccgcg acggaaccga ggcggtcagc gcggcggccc agcgcctata
     3421 cgaatccttg ccgcgctcgg tgcagcgccc aaggataagt tccgcccgcg atgacgtcgg
     3481 accagcctgg gtggccgcgg ccgtttc
//
LOCUS       DAOOLN010000044         3463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_32966_length_3463_cov_3.408451, whole genome shotgun sequence.
ACCESSION   DAOOLN010000044 DAOOLN010000000
VERSION     DAOOLN010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32966_length_3463_cov_3.408451"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2196
                     /gene="mutS"
                     /locus_tag="PLW80_00950"
     CDS             <1..2196
                     /gene="mutS"
                     /locus_tag="PLW80_00950"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPB65097.1"
                     /translation="WADASTGEFKAGSFGRGDPERLRRELFRLSPREALVRESTLDDD
                     GVARALSERPAIVLNRFPDWTFSLEQGRSTLLGHFGTSSMKGFGFDDGDPALAAAGAL
                     LEYLRDSVKARLSQFRTLGGCDDAEFVSIDESSQKNLELDRNLRDGGAAFTLYGVVNH
                     TKTAMGARALRSRLQRPLRSAAAVNGRLDAVDALYRDQRSLERVRAILGSCRDLERLA
                     SRLAMDRASPKDLVALRDTIRAALSLGPALPIAAPTALSTLAEPGDRDAALAFADVIA
                     RAVVDDPAMSPADGSVIREGWSSELDGLRSLRSDAHGVLEAYLEEERRSSGLSGLRVR
                     YNKIIGYYLELSKGAAQSAPAHFIRRQSTTNTERFTTERLGQLESDIHGAAERIVELE
                     RSLFSALCEELKPSAPLLFETAAELAELDCAASFAWVATARGYVRPTVDESLVLEVRG
                     GRHPVVEAYLPSGEFVPNDLLLDGDGTGFALITGPNMAGKSTFLRQNALIALLAQAGS
                     FVPASSARIGVVDRIFCRVGAQDNLARGESTFLLEMHETASILNNVGARSLVVMDEVG
                     RGTGTLDGLSIAWAVSEHVLDACRSRTLFATHYHELTALRHERLVDLSMAVEEREGEV
                     VFLKRIRSGAATGSYGIHVARLAGVPLAVVARAMEIKATLEAGEARLAVDDIPEVGGA
                     TLPREPEAGGLFSVEELVLDELRSLDLDRITPIEALSRLAAAQKALRGR"
     gene            2368..3054
                     /locus_tag="PLW80_00955"
     CDS             2368..3054
                     /locus_tag="PLW80_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPB65098.1"
                     /translation="MRCVICGLPADADSRYPLCARCAGRLLDEALESAELSRPAARDP
                     SRCPVCGKLAVSELGPCMRCRSAGYQFDSALPLLRYSGDARAALLSYKAGRTSLASFF
                     ADALAVALSGHYSGRTVVPVPPRPGKIRRYGWDQVERIARILERRHGIAVARVLKRAG
                     GHQQKSLGLEARAVNMRGAISLRRGAEAPLAPVLLDDVMTTGATLSACADALKGAGAG
                     RVDAIVIAAD"
     gene            3186..>3463
                     /locus_tag="PLW80_00960"
     CDS             3186..>3463
                     /locus_tag="PLW80_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HPB65099.1"
                     /translation="MTESIELYNDIASLLSGIGVELVELHLSGKRGGTKASAVVYKSG
                     GTGIDECSAAHRLIQARLEVLLGTDDFQLETASPGVDRVIRSPAEYAI"
BASE COUNT          542 a         1153 c         1186 g          582 t
ORIGIN      
        1 tgggccgacg cttcgaccgg cgagttcaag gcaggctcgt tcgggcgcgg cgatcctgag
       61 cggctgcgca gggagctgtt caggctgtcg ccgcgcgagg ccctcgtccg ggagtcgacc
      121 ctcgacgacg acggcgtggc gcgcgcccta tccgagcgac cggctatcgt gctgaaccga
      181 ttcccggact ggaccttctc gctcgagcag ggcaggtcga ccctgctcgg ccacttcggc
      241 acgagcagca tgaagggctt cggcttcgac gacggcgacc cggcgctggc cgcggctggc
      301 gccctgctcg agtacctgcg cgattcggtc aaggcgcgtc tgtcgcagtt caggaccctc
      361 ggcggttgcg acgacgccga gttcgtgtcg atcgacgagt cgagccagaa gaacctcgag
      421 ttagaccgta acctgcgcga cggtggggcg gcgttcacgc tgtacggcgt cgtcaaccac
      481 acgaagacgg ctatgggcgc tcgggcgctc cgctcgaggc tgcagcgccc gctccggtcg
      541 gccgccgcgg tcaacgggcg gctcgacgcc gtcgacgcgc tgtatcgcga ccagcgatcg
      601 ctcgagcgtg tacgcgcgat actcggttcg tgccgcgacc tcgaacgcct ggcctcgagg
      661 ctggcgatgg accgcgcctc gcccaaggat ctcgtcgctc tacgggacac gatccgggcc
      721 gcgctgtcgc tgggtccggc cctgccgata gccgcgccga cagccctgtc gacgctcgcc
      781 gaaccaggcg atagagacgc ggccctggcc ttcgccgacg tcatagcgcg ggccgtggtc
      841 gacgacccgg ccatgtcccc cgccgacggc tccgtgatac gggagggctg gagctccgag
      901 ctcgacggtc tacggtcgtt gcggtcggac gcccacggcg tgctcgaggc ctacctcgag
      961 gaagagcggc ggtcctcggg gctttcgggg ctgcgggtca ggtacaacaa gatcataggc
     1021 tactacctgg aactgtccaa aggcgctgcc cagtcggctc cggcgcactt catccgacgg
     1081 cagtcgacga cgaacaccga gcgcttcacg accgagcggc tcggccagct ggagtcggat
     1141 atccacggcg cggccgagcg tatcgtcgag ctggagagat cgctcttctc cgctctgtgc
     1201 gaggagctca agccgtccgc gccgctgtta ttcgagacgg ccgccgagct ggccgagctg
     1261 gactgcgccg cgtcgttcgc ctgggtcgct acggcccgag gctacgtccg ccctaccgtc
     1321 gacgaatcgc tggttctcga ggtgcgggga gggcggcatc ccgtcgtcga ggcgtacctg
     1381 ccctcgggcg agttcgtacc gaacgaccta ctcctcgatg gcgacggaac aggcttcgcc
     1441 ctaatcaccg gcccgaacat ggccggtaag tcgacgttcc tcaggcagaa cgcgctcatc
     1501 gcgctactcg cgcaggcggg ctccttcgtg cccgcctcgt ctgccaggat aggcgtcgtc
     1561 gacaggatct tctgccgcgt cggcgcccag gacaacctgg ccaggggcga gtctaccttc
     1621 cttctcgaga tgcacgagac ggcgagcatt ctgaacaacg tcggcgcgcg tagcctcgtc
     1681 gtgatggacg aggtggggcg cggcaccggc acgctggacg gcctgtcgat agcctgggcc
     1741 gtcagcgagc acgtcctcga cgcgtgccgt tcgaggaccc tgttcgccac gcactatcac
     1801 gagttgacgg cgctcaggca cgagcggctc gtcgatctgt cgatggccgt ggaggagcgc
     1861 gagggcgagg tggtcttcct caagcggatc cggtccggcg cggcgaccgg gtcatacggc
     1921 atccacgtgg ccaggctggc cggggtgccg ctcgcagtcg tcgcccgggc catggagatc
     1981 aaggcgacgc tcgaagccgg cgaggccagg ctcgccgtcg acgatatacc cgaggtcggc
     2041 ggcgctacgc tcccgaggga accggaggcc ggcgggcttt tctcggtcga ggaactcgta
     2101 ctcgatgagt tgcgatcgct ggacctggac cgtatcacgc cgatcgaggc cctttccagg
     2161 ctggcggccg cccagaaagc cttacggggg cgctgaagcg gatgcatgag ggaaccggaa
     2221 cgagagccgt aacggggccg gaaggggaac cgttacgggt gaatgcgccg tgaaccttcg
     2281 cggcgtacaa tagacatggt acgacctgat cgagttgggt acgcgtccct ccgagcgctc
     2341 cggcgagccg tctcgacgtt cgcgccgatg cgttgcgtca tctgcggctt gcctgccgac
     2401 gccgatagcc gctatccgct gtgcgcccga tgcgcggggc ggcttctcga cgaagcgctc
     2461 gagtcggcgg agctctcacg gccagccgcc cgagatcctt cgcgctgccc cgtctgcggc
     2521 aagctcgccg tatcggagct cggcccctgc atgcgctgcc ggtcggctgg ctaccaattc
     2581 gactcggccc tgccgctcct gcgctattcc ggagatgcgc gggccgcgct cctgtcgtat
     2641 aaagctggac gcacgagcct cgcctcgttc ttcgccgacg ccttggccgt ggccctatcg
     2701 gggcattact cagggagaac ggtcgtaccc gtgccgcctc ggcccggcaa gatacgacgt
     2761 tacggctggg atcaggtcga acgcatcgcc cgtatactgg agagacgcca cgggatcgcc
     2821 gtagccaggg tgctcaagcg cgcgggcggc caccagcaga agagccttgg cctcgaggct
     2881 cgcgcggtga acatgcgcgg cgccatatcg ctacggcgcg gagcggaagc gcctctcgcg
     2941 ccagtcctgc tcgacgacgt gatgacgacc ggcgcgaccc tgtccgcctg cgccgacgcg
     3001 ctcaagggcg ccggcgcggg gcgtgtcgac gccatcgtca tagcggccga ctgatagatg
     3061 gccatggcga ggggcctgcg gcgtattgac ttagcgtgac gcgaaggcta tgatggctac
     3121 agacatggtt tttcttagtt tgaatgtaag aagagtcgtt cccacggctc tttttttata
     3181 ggtgtatgac ggagtcaata gaactctaca atgatatagc ctctctcctc tcggggattg
     3241 gcgtcgagct ggtcgaattg cacctgagcg gaaaacgcgg cgggacgaag gcgtcggccg
     3301 tcgtgtacaa gtcgggcggc accggcatcg acgagtgctc cgcggcgcac cgcctcatcc
     3361 aggctcgcct ggaggtcctg ctcggtaccg acgacttcca gctggagacc gcctccccgg
     3421 gcgtcgaccg cgtaatccgc tccccggcgg agtacgccat att
//
LOCUS       DAOOLN010000045         3456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_33053_length_3456_cov_3.072332, whole genome shotgun sequence.
ACCESSION   DAOOLN010000045 DAOOLN010000000
VERSION     DAOOLN010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33053_length_3456_cov_3.072332"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PLW80_00965"
     CDS             complement(<1..449)
                     /locus_tag="PLW80_00965"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPB65100.1"
                     /translation="MSGAMVVILMGSKSDEAHCATIAEALDALGVAHESRVGSAHKTP
                     ARVLAMLAEYEASGRPRVYITVAGRSNALSGFVDGAVEAPVIACPPPSDAFGGADLYS
                     SLRMPSGVAPALVLDPANAALAAAKILGLSDPAVAARVRAFREANARA"
     gene            complement(446..1384)
                     /locus_tag="PLW80_00970"
     CDS             complement(446..1384)
                     /locus_tag="PLW80_00970"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPB65101.1"
                     /translation="MTFEGCDLGLAGKRSGKVRDWYPLPGGRRLIVTTDRLSAFDRTL
                     AAVPYKGQVLNQLSAWWFERTRDIVGNHLLSVPDPNAVVALEAEPLPIEVIVRGYVTG
                     VTSTALWYRYSLGERSIYGYDFPDGLRKNQALPEPIITPTTKGGPTGHDERLTGVEVV
                     SGGYLDEADWATVSRAALALFRRGQETAAAAGLLLVDTKYEFGRAPDGSIILIDEVHT
                     PDSSRFWKAESYRSRFEAGHEPECFDKEYVRLAFAELGYRGDGPVPPVRDSLWVETSS
                     LYRSAYERLTGESFVPGESPVGPRLERNLRAAGLLS"
     gene            complement(1606..2388)
                     /gene="purQ"
                     /locus_tag="PLW80_00975"
     CDS             complement(1606..2388)
                     /gene="purQ"
                     /locus_tag="PLW80_00975"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002677984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HPB65102.1"
                     /translation="MRRPKAIVLHAAGTNRDPDAYVALGLSGADPEIVHVNQLRSGER
                     RLTDYRLLVIPGGFSYADALGAGRTFALDLASYFADEVAEFVASGKPVIGICNGFQVL
                     VKAGILPGFGIGGRRLATLARNDNGRFECRWSTLLAPSSTCVWTRSLPGPLACPIAHG
                     EGRFVVDAEATLEKLRSAGQIALVYGDDDGRPANGEYPANPNGSVADVAGICNPAGNV
                     LGLMPHPENNVVVRARDGEARRERTRACLALWRNGVEHAKEL"
     gene            complement(2385..>3456)
                     /locus_tag="PLW80_00980"
     CDS             complement(2385..>3456)
                     /locus_tag="PLW80_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPB65103.1"
                     /translation="ATAPSSGEALLAILAHPAVRSREATVRLYDHEVQGATMLGPYAG
                     LKADGPQDAAVLRPRESRGQVAVSLSNGFNHRYGAADPYRMAVSALDEAVRNAVAVGA
                     DPDRIAVLDNFCLGDPTVPETMWTLLEAARGCYDAATAHRTPFISGKDSFNNAYLAPD
                     GRRVSVPPSLLISAIGIVPDAGAVPGSDLKAPGDRLYLVGTMEPSFGGSVYADLYGVP
                     DGAAPGVPAASPGAPMAYRALHRAIAAGLVRACHDLSDGGLATALAEMCLGGRLGAKA
                     TSPGDGLVAAALFGETNGCLVVEVADTAAAEFERIMAGIECRRIGETDGGGRLELVAG
                     DEGFAVALDDLAVAFGGTRSER"
BASE COUNT          516 a         1193 c         1198 g          549 t
ORIGIN      
        1 gccctggcgt tggcctcgcg gaaggccctg acccgggcag cgacggccgg gtccgacagg
       61 ccaaggatct tggcggccgc cagcgccgcg ttggccgggt ccagcaccag ggccggcgct
      121 acgccgctcg gcatccgtag cgacgagtag aggtcggcgc cgccgaaggc gtccgacggc
      181 ggcgggcagg cgatcaccgg cgcctccacc gcgccgtcga cgaaaccgga cagcgcgttg
      241 gacctgccgg cgacggttat atagacgcgc ggccggcccg aggcctcgta ctcggcgagc
      301 atggccagca cgcgcgcggg cgtcttgtgc gccgagccga cccggctctc gtgggccacg
      361 ccgagcgcgt cgagggcttc ggctatcgta gcgcagtgcg cttcgtcgga cttggagccc
      421 atcaggatga cgaccatggc gccgctcacg atagcagccc cgcggcgcgc aggttgcgct
      481 cgagcctcgg cccgaccggg gattctcccg gcacgaagga ctcgccggtc aagcgctcgt
      541 aggccgagcg gtacagcgag ctcgtttcga cccagagcga gtcgcgcacg ggcggtaccg
      601 ggccgtcgcc gcggtagccc agctccgcga acgccagccg cacgtattcc ttgtcgaagc
      661 attccggctc gtgcccggcc tcgaatcggg agcggtagct ctcggccttc cagaagcgcg
      721 acgagtcggg cgtatgcacc tcgtctatca gtatgataga gccgtccggg gcgcggccga
      781 actcgtactt cgtgtcgacg agcaggagcc cggcggccgc ggccgtctcc tggccgcgcc
      841 tgaacagcgc cagggccgcg cggctcaccg tcgcccagtc cgcctcgtcg aggtagcctc
      901 cggagaccac ctcgaccccg gtcagccgct cgtcgtgccc ggtcggcccg cccttcgtcg
      961 tcggcgtgat gatgggctcg ggcagggcct ggttcttgcg caggccgtcc gggaaatcgt
     1021 agccgtagat cgatcgctcg ccgagcgagt agcggtacca gagggcggtc gaggtgacgc
     1081 cggtgacgta gccgcgcacg atcacctcga taggcagcgg ctcggcctcg agcgccacga
     1141 cggcgttcgg gtcagggacc gacagcaggt ggttgccgac gatgtccctc gtccgctcga
     1201 accaccaggc cgacagctgg ttgagcactt gccctttgta cggtaccgcc gccagcgtcc
     1261 ggtcgaacgc cgaaagtcgg tcggtggtga cgataagccg ccgcccgccc ggtagcgggt
     1321 accagtccct caccttgccc gagcgcttgc cagccaaccc tagatcgcat ccctcgaacg
     1381 tcatgttcac ccctctaagt atcattgata atttgaacca cggagacgcg gaggcacaga
     1441 gagtattctt ttatatctgt atactcatca ccttcaccga gcctccgccc tccccaaatc
     1501 ctctctttaa tccttcttcg ccttctccgt gtccccgtgc ctccgtggcc caattcccct
     1561 attcaactct tcttcttttt atccgtgtcc ccgtggttag aaccctcaca actccttcgc
     1621 gtgctcgacg ccgttccgcc acagggccag acaggcgcgg gtgcgctcgc ggcgggcctc
     1681 gccgtcgcga gcgcggacga cgacgttgtt ctccgggtgc ggcatgagcc cgagcacgtt
     1741 gcccgcgggg ttgcagattc cggcgacgtc cgccacggaa ccgttcgggt tggccgggta
     1801 ctcgccgttg gcgggtcttc cgtcgtcgtc gccgtagacc agcgctatct gacccgcgga
     1861 tcgtagcttt tcaagggtgg cctccgcgtc gacgacgaag cgtccctcgc cgtgggctat
     1921 cgggcaggcg agcggcccgg gcaggctccg ggtccagacg caggtcgacg atggggccag
     1981 cagcgtcgac caacggcact cgaagcggcc gttgtcgttg cgcgcgagtg tcgcgaggcg
     2041 ccgaccgccg atcccgaagc cgggcagaat gccggccttg acgagcacct ggaagccgtt
     2101 gcagatgcct atcacgggct tgcccgaggc gacgaactcg gcgacctcgt cggcgaagta
     2161 cgaggccagg tcgagcgcga aggtccgtcc ggcgccgagc gcgtcggcgt acgagaagcc
     2221 gccgggtatg acgagtagcc ggtaatcggt caggcggcgc tctcccgagc gtagttggtt
     2281 gacgtgtacg atctccgggt cggctccgga caaaccgagg gccacgtagg cgtccgggtc
     2341 gcggttcgtg cctgcggcgt gtagcactat cgccttgggt cgtctcatcg ttcgctcctc
     2401 gttccgccga aggccacggc caggtcgtcg agcgcgacgg cgaagccttc gtcgcccgcg
     2461 accagttcga ggcggcctcc accgtcggtc tcgcctatcc tgcggcattc gataccggcc
     2521 atgatgcgtt cgaactcggc ggcggccgta tccgcgacct cgacgacgag gcagccgttc
     2581 gtctcgccga acaacgccgc ggcgaccaag ccgtcgccgg gcgaggtagc cttggcgccg
     2641 agccgccctc cgaggcacat ctcggcgaga gccgtcgcca ggccgccgtc ggatagatcg
     2701 tggcaggcgc gtacgaggcc ggcggcgatg gcccggtgga gggcgcgata ggccatgggc
     2761 gcccctggcg aggcggcggg tacgcccggg gcggcgccgt cggggacgcc gtacaggtcg
     2821 gcgtagaccg agccgccgaa cgacggctcc atggtgccta cgaggtacag cctgtcgccc
     2881 ggcgccttga ggtccgaacc tggcacggcc ccggcgtcag gcacgatgcc gatagccgag
     2941 atcaggagcg agggaggcac cgataccctg cggccgtccg gagccaggta ggcgttattg
     3001 aaggagtcct tgccggatat gaagggcgtc cggtgcgccg tcgccgcgtc gtagcaaccg
     3061 cgggccgcct cgaggagcgt ccacatcgtc tcgggtaccg tcgggtctcc gaggcagaag
     3121 ttatcgagca ccgcgatccg gtcggggtcg gcgccgacgg ccaccgcgtt gcgtacggcc
     3181 tcgtcgaggg cggagaccgc catgcgatag gggtcggccg cgccgtagcg atggttgaag
     3241 ccgttcgaca gggagaccgc cacctgaccc ctcgactcgc gcggccggag cacggcggcg
     3301 tcctgcgggc cgtcggcctt gaggcccgcg taggggccga gcatggtggc gccttgcacc
     3361 tcgtggtcgt agagcctcac ggtggcttct ctggaacgaa ccgccggatg ggccaggatg
     3421 gcgagcaggg cctcaccgct cgatggagcc gtagcc
//
LOCUS       DAOOLN010000046         3454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_33075_length_3454_cov_2.432186, whole genome shotgun sequence.
ACCESSION   DAOOLN010000046 DAOOLN010000000
VERSION     DAOOLN010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33075_length_3454_cov_2.432186"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /gene="fusA"
                     /locus_tag="PLW80_00985"
     CDS             <1..616
                     /gene="fusA"
                     /locus_tag="PLW80_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257779.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPB65104.1"
                     /translation="HKKQTGGSGQFARIAGYMEPIEGAVYEFVDSVKGGAIPNEYIPS
                     CDKGFKEAAKKGSLIGFPVVNIRCVINDGASHPVDSSDIAFQLAAIGCFREAYEKAKP
                     VILEPIMKVVAEGPAEFQGNIFASVNQRRGVITASTEEASFCRVEAEVPLSEMFGYST
                     VLRSMTQGKAEFSMEFLKYGKVPQSVSEQLRKDYEEKRKKEQGR"
     gene            786..1490
                     /locus_tag="PLW80_00990"
     CDS             786..1490
                     /locus_tag="PLW80_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65105.1"
                     /translation="MLKEELIKKSPVRVLERGIEGGLKAGNIGVIASRKGVGKTSVLV
                     QLALDKLMQGKKVIHVSFTTHTSYVISWYENIFTELAKKRNLEDHSSVHDDLMKNRVI
                     MNFNQEGVTTDQILKSLRAMIVDGGFDADAIIADGFDFARADAEHFSKVRAFAEEMKL
                     EFWYSCTMSGEEPLLDKKNVPVILRPFLDVISVLIVLEPKAEYIHFIVVKDHGRMNPE
                     DLNIKLDSKTLLIAEA"
     gene            complement(1965..2453)
                     /locus_tag="PLW80_00995"
     CDS             complement(1965..2453)
                     /locus_tag="PLW80_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65106.1"
                     /translation="MATSIHARFLQDARTLLEQASGLPELASELKEYGYTPGKFQEGF
                     KLLEEAEVLSRRKAVEYGEKYESSAKLVKAWKGANVLYQKALKVARVALRDAPKSTEA
                     LGLGGPRNLSVSGWYEQAGDFYANLLANDGYVRALGAFGYSRERLVEERRVIEAVMDT
                     RD"
     gene            complement(2925..>3454)
                     /locus_tag="PLW80_01000"
     CDS             complement(2925..>3454)
                     /locus_tag="PLW80_01000"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HPB65107.1"
                     /translation="LLVPGDERLEPRGPSDLNDTMLRRAFAPALDFGAAELAAWNVSA
                     GERAAVAAMAALVASIASGLPSAAPLFDRCYLGLGADGHVAGVFPGSPAARRAGSRSA
                     PIAVAGTAPEEPRARVSLSLPTLASTRGTRFIVRAAGKEDALRRLASGDPACPAVAAA
                     AEGALAFVLARQARP"
BASE COUNT          605 a         1104 c         1165 g          580 t
ORIGIN      
        1 gcacaagaag cagaccggcg gctcgggcca gttcgcccgc atcgccggct acatggagcc
       61 tatagagggc gcggtctacg agttcgtcga ctccgtcaag ggcggggcta taccgaacga
      121 gtacataccg tcctgcgaca agggcttcaa ggaggcggcc aagaagggct cgctgatcgg
      181 cttcccggtg gtcaatatcc gctgcgtgat caacgacggc gcgagccacc cggtcgactc
      241 ctccgacatc gcgttccagc tggccgcgat cggctgtttc cgcgaggcct acgagaaggc
      301 caagccggtg atcctcgagc cgatcatgaa ggtggtcgcc gagggccccg ccgagttcca
      361 gggcaacatc ttcgcgtcgg tcaaccagag gcgcggcgtg atcacggcca gcaccgagga
      421 ggcctcgttc tgccgcgtcg aggccgaggt gccgctgtcc gagatgttcg gctactcgac
      481 ggtgctgcgc tcgatgaccc agggcaaggc cgagttctcg atggaattcc tcaagtacgg
      541 caaggtgccc cagagcgtct ccgagcagct ccgcaaggac tacgaggaga agcgcaagaa
      601 ggagcagggc cgctagccgc gaggcggcgc ccgtagcggg cgggccggaa ttctccggcc
      661 cgtttgcgtt ttgtacggaa cggccgtata ctcgtatacg tggtacaccg agagcggccc
      721 gccggggccg cgttgaaccg atcaatatac tacacgacac ctaaccgaca tcgtaaggag
      781 ttcccatgct gaaagaggag ctgatcaaga agagtcccgt tcgcgtcctc gagcggggca
      841 tcgagggcgg cctcaaggct ggtaatatcg gcgtcatcgc gagccgcaag ggcgtaggca
      901 agacgagcgt gctggtccag ctggccctcg acaagctcat gcagggcaag aaagtcatcc
      961 acgtctcctt caccacccac acgagctacg tcatctcgtg gtacgagaat atcttcaccg
     1021 agctcgccaa gaagcgcaac ctcgaggacc actcgagcgt ccacgacgac ctgatgaaga
     1081 accgcgtgat catgaacttc aaccaggagg gcgtgaccac cgaccagatc ctcaagtcgc
     1141 tccgcgcgat gatcgtcgac ggcggcttcg acgccgacgc catcatcgcc gacggcttcg
     1201 acttcgcccg cgccgacgcc gagcacttct ccaaggtccg cgccttcgcc gaggagatga
     1261 agctcgagtt ctggtactcc tgcaccatgt ccggcgagga gccgctcctg gacaagaaga
     1321 acgtgccggt tatcctccgg cccttcctcg acgtgatctc cgtgctgatc gtcctcgagc
     1381 ccaaggccga gtacatccac ttcatcgtcg tcaaggacca cgggcgcatg aaccccgagg
     1441 acctgaacat aaagcttgac tcgaagactc tcctgatcgc cgaagcctag ttttcgcgcg
     1501 tagcgaggcg tctacttcgt cgcccccgaa gggaagcccc gatccgttcc tctcgacgtg
     1561 caaggagcac gccgtcgagg cccggatcgg ggcgcttctc gtatacatcc tcgctacgcg
     1621 cgaaaacccg agtagagtga gacataccgg ggcgatcgcc tcgtctctcg ccccgaaggg
     1681 aaccccccgg gcccctcgac gtgcagagag cacgccgtcg tggcccgatc cgttgaaccg
     1741 gatcggggcg ttgctcgtcc ctacgccgcc ttgaccttct tcttgccgcc gctatgcctg
     1801 cgtacggtcg gcgcgagggg gccgagcttc ggtagctcgt cgcgcttctc gtagaaggcc
     1861 acctcgcaga tcgcgcggag gtcggagacc cagtcgtcca gttccttgag cttccggtcg
     1921 cgggcggcgg taaccgcctt ggcttgggcg gcctcgatag ccgtctagtc cctcgtatcc
     1981 atgacggcct cgataacccg ccgttcctcg acgaggcgct cgcgggaata gccgaaggcg
     2041 cccagggctc gtacgtagcc gtcgttggcc aacaggttcg cgtagaagtc gccggcctgc
     2101 tcgtaccacc cggagacgct caggttgcgc gggccgccta gaccgagagc ctcggtgctc
     2161 ttgggggcgt cgcgcagggc cacgcgggcc accttcaggg ccttctggta caggacgttg
     2221 gcgcccttcc aggccttgac gagcttggcg ctgctctcgt acttctcgcc gtactcgacc
     2281 gccttgcgcc tggacagaac ctcggcttct tcgagcagct tgaacccttc ctggaacttg
     2341 cccggcgtgt agccgtactc cttcagctcc gaggccagct ccggtagccc cgatgcctgt
     2401 tccaggagag tcctggcgtc ctgaaggaac ctggcatgaa tcgatgtggc catcgcccac
     2461 ctccaaacgt gtattggtgc cgccaatgac ggctttttgt actcgggagc cgtgtagccg
     2521 gacatggaag cggccttgcc tggccgggga gcgatattcg gcgatcgatg agcgatattc
     2581 ggcgatcaat gggcgatctt ccgagatcga tgggcgatct tccgggatcg aggagcgatg
     2641 ttccgggatc gatgagcgat attcggcgat cgatgagcga tgttccggga tcgatgggcg
     2701 atgttccggg atcgatccgt catggtcgga tggaatcagg gggcaggcgt cgcttgctat
     2761 gattaggagc ctccggtcag cgcgggttcg acacggcgaa ttgctgcagg ctcccgatgt
     2821 ggatgcttcc gttcgcccgt ggcggctgga agccatgggc tcagtgtagg ccggatcagg
     2881 gattcgtcaa gggaaagttc tgatagggag tggcgtgaag ccgattatgg ccgcgcctgt
     2941 cgggcgagca cgaacgccag agcgccttcg gccgcggcgg cgaccgccgg gcaggccggg
     3001 tcgccggagg ccaggcggcg gagcgcgtcc tccttgccgg cggcgcggac gatgaagcgc
     3061 gtgcctcggg tcgaggcgag ggtcgggagg gacaggctga cgcgggcgcg cggctcctcg
     3121 ggcgccgtac cggcgacggc tatgggcgcg gaacgggaac cggcccgacg ggcggccggg
     3181 ctgccgggga agacgccggc gacgtggccg tcggcgccga ggcccaggta gcagcggtcg
     3241 aagagcggcg cggccgacgg caggccggag gcgatggacg cgacgagggc ggccatcgcc
     3301 gcgacggcgg cgcgctcccc ggcggagacg ttccaggcgg ccagctcggc cgcgccgaag
     3361 tccagggccg gggcgaaggc gcgcctgagc atcgtgtcgt tcaggtcgga gggcccgcgt
     3421 ggctcgagcc gctcgtctcc gggcacgagc agga
//
LOCUS       DAOOLN010000047         3452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_33096_length_3452_cov_2.261996, whole genome shotgun sequence.
ACCESSION   DAOOLN010000047 DAOOLN010000000
VERSION     DAOOLN010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33096_length_3452_cov_2.261996"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..383)
                     /locus_tag="PLW80_01005"
     CDS             complement(81..383)
                     /locus_tag="PLW80_01005"
                     /inference="COORDINATES: protein motif:HMM:NF016974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HPB65108.1"
                     /translation="MSYSSSLGTGVTNTRAIVHLALDIPPGCGFRVGGETREWIEGKA
                     FAFDDTIEHEAWNTSDAPRAILILDCWNPHLCEGERAAITAWFAASDAEIGPGKPL"
     gene            complement(403..1914)
                     /locus_tag="PLW80_01010"
     CDS             complement(403..1914)
                     /locus_tag="PLW80_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65109.1"
                     /translation="MSEEQKAEDSGKGQDRKPEYTPPKGAVKQLSVKAGGRSLEYRAE
                     ADWIVLRKDEKPTAEMFYTYYSVEGDDGRPVTFVFNGGPGASSVYLHFGAIGPKRAEF
                     GGGGEPLPPPHRLIDNDETWLRFTDLVFIDPIGTGLSRMVPDDKAEAAKKDQGAAPDA
                     KKDVEYWGIKRDLESIGEFVSKFLSRYKRWESPVYLAGESYGGFRVARLARQLQEQYG
                     VALAGAVIISPALEFSLLDASDYDDLPWVDSFATMAGAAAYHGRARKQKPGESIRDYA
                     TRASGFAVRELLPVLAAGDFLGRARKDRTLDAAADYIGLPRAVVRAKYGRVTIDYFVK
                     NLLRDKGLVVGLYDASAVVRDPYPDRDHWSGPDPTLHVIERVFAAAINTQLRANIGLE
                     TERDYTLSSDEANENWKNDMRKHALESQTGSTDELRYGMSINPHMKVYLTHGVYDLVT
                     PYFAAERIKRLMKLDDERERMLTVRYYEGGHMFYTWDDSRKAFARDMEAFYGA"
     gene            1995..3026
                     /locus_tag="PLW80_01015"
     CDS             1995..3026
                     /locus_tag="PLW80_01015"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HPB65110.1"
                     /translation="MTINRVAVRNGLGRAIPAVATLILCASCATGWHGEERDFRGDMR
                     ELVQDVSAYAHDSRPGFIVIPQNGHQLLTLDGQSSGATASDYLAAIDGVGREDLFYGY
                     DGDDVATPAPERDAMLAFMDLAEANGIQVLATDYCSTPAKVDDSYARSASRGYISFAA
                     ERRELDGVPAYPAAPMNAQPAVDVSSLASARNFLYLINPGAYATKDAYLSAIDASDYD
                     LVIMDLFYEDADGVATALSQTDLDTIRDKSGGGRRLLVCYMSVGEAEDYRYYWDESWD
                     DEPPSWIAGENPYWAGNYKVEYWDSGWKAVLYGSSGAYLDRVIAAGFDGVYLDIIDAF
                     EYFEEALGL"
     gene            complement(3089..>3452)
                     /locus_tag="PLW80_01020"
     CDS             complement(3089..>3452)
                     /locus_tag="PLW80_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPB65111.1"
                     /translation="KMRLSFIDGDNYVFMDQESFEQYELTKDDLGDNAQYITPEDDFD
                     VEITFYEGKPVGVVLPISIERTITYCEPGIKGDTSGKSSKPATLDSGCVVQVPLFCDI
                     GTRIRLDTRDGSFQERVK"
BASE COUNT          568 a         1105 c         1149 g          630 t
ORIGIN      
        1 ggccgagaac catcgaaatc agcatgtctg tctccgcgaa aacgggatat tgcgagagac
       61 tatcagaatg ctgcgatcgc tcaaagcggt ttgccgggcc ctatttccgc gtccgacgcc
      121 gcgaaccatg cggtgatcgc ggcacgctcg ccttcgcaca ggtgcgggtt ccagcaatcg
      181 aggatcagga tcgcgcgggg cgcatcgctg gtattccacg cctcgtgctc gatcgtatcg
      241 tcgaacgcga aggccttgcc ctcgatccac tcgcgcgtct cgccgcccac ccggaacccg
      301 cagccgggcg gaatgtcgag tgcaaggtgc acgatcgcgc gggtattggt gacgccggtg
      361 ccgagcgacg atgagtatga catagctgac ctccccggta gcctaggccc cgtagaacgc
      421 ttccatgtcg cgggcgaagg ccttacggct gtcgtcccag gtgtagaaca tgtgcccgcc
      481 ctcgtagtac ctgacggtca gcatgcgctc tcgctcgtcg tcgagcttca tgagccgctt
      541 gatgcgctcg gccgcgaagt acggcgtgac caggtcgtac acgccgtggg tcaggtagac
      601 cttcatgtgc gggttgatgc tcatgccgta gcgcagctcg tcggtcgagc cggtctggct
      661 ctcgagcgcg tgcttgcgca tgtcgttctt ccagttctcg ttggcctcgt cgctcgacag
      721 cgtgtagtcg cgctcggtct cgagcccgat gttggcgcgg agctgggtat tgatcgcggc
      781 ggcgaacacg cgctctatca cgtgcagggt cgggtccggg ccggaccagt ggtcgcggtc
      841 cgggtacggg tccctgacta cggcgctcgc gtcgtagagg ccgacgacga ggcccttgtc
      901 gcgcagcagg ttcttgacga agtagtcgat ggtcacgcgg ccgtacttgg cccggacgac
      961 ggcccgaggc aggccgatgt agtcggcggc ggcgtcgagc gtgcggtcct tgcgggctcg
     1021 ccccaggaag tcgccggcgg ccagcacggg cagcagctcg cgtaccgcga agccggacgc
     1081 gcgcgtcgcg tagtcgcgta tgctctcgcc gggcttctgc ttgcgcgcgc ggccgtggta
     1141 ggcggcggcg ccggccatcg tcgcgaagct gtccacccag ggcaggtcgt cgtagtctga
     1201 cgcgtcgagt agcgagaact cgagggccgg cgatatgatc acggcgccgg cgagcgccac
     1261 gccgtactgc tcctggagct ggcgcgcgag cctggctacg cggaagccgc cgtagctctc
     1321 gcccgcgagg tagacggggc tctcccagcg cttgtagcgc gacaggaatt tggatacgaa
     1381 ctcgcctatg gactcgaggt cgcgcttgat gccccagtac tctacgtcct tcttggcgtc
     1441 gggcgccgcc ccctggtcct tcttggctgc ctcggccttg tcgtcgggca ccatgcgcga
     1501 cagccccgtg ccgatcgggt cgatgaagac gaggtcggtg aagcgtagcc aggtctcgtc
     1561 gttgtcgatg aggcgatgcg gaggaggcaa cggttcgccg ccgccgccga actcggcccg
     1621 tttggggccg atggcgccga aatgaaggta gaccgacgag gcgccgggcc cgccgttgaa
     1681 gacgaaggtg acgggacggc cgtcgtcgcc ctcgacgcta tagtacgtat agaacatctc
     1741 ggcggtcggc ttctcgtcct tgcgcaggac tatccagtcg gcttccgcgc ggtactcgag
     1801 gctcctgccg ccggccttga cagacagttg cttgaccgct cccttgggcg gagtgtactc
     1861 gggcttcctg tcctggccct tgcctgaatc ctcggctttc tgctcctctg acatggctac
     1921 ctccttgaac gcgggctata gtatagcacg aggcgcgctt ggccgtaacg ccgcgcgttc
     1981 atagggaggc tagcatgaca atcaatcgag tcgcggtacg gaacggccta gggcgcgcta
     2041 tcccggcggt agccacgctt atactctgcg cgtcctgcgc caccggctgg catggcgaag
     2101 agcgcgactt caggggcgac atgcgcgagc tcgtccagga cgtatcggcc tacgcccacg
     2161 atagccgtcc cggtttcata gtcatcccgc agaacggcca tcagctgctt acccttgacg
     2221 gccagtcctc cggcgctacc gcgtcggact acctggcggc catagacggc gtgggccgcg
     2281 aggacctctt ctacggctac gatggcgacg acgtggcgac gcccgccccg gagcgggacg
     2341 ccatgctggc cttcatggac ctggccgagg ccaacgggat ccaggtgctg gctacggact
     2401 actgctcgac cccggccaag gtggacgact cgtacgcgcg gagcgcctcc aggggctata
     2461 tatcgttcgc ggccgagcgg cgcgaactcg acggggtccc cgcctacccg gccgcgccga
     2521 tgaacgccca gcctgccgtc gacgtatcgt cgctagcgtc cgcgcgcaat ttcctgtacc
     2581 tcataaaccc cggcgcctac gccacgaagg acgcgtacct aagcgctatc gacgccagcg
     2641 actacgacct cgtcatcatg gacctgttct acgaggacgc cgacggcgtg gcgacggccc
     2701 tgagccagac ggacctcgat acgattcgcg acaagtccgg aggaggcagg aggctgctcg
     2761 tctgctacat gagcgtcggc gaggcggagg actaccggta ctactgggac gaatcatggg
     2821 acgacgagcc gccctcctgg atagccggcg agaatccgta ctgggcaggc aactacaagg
     2881 tcgaatactg ggactcaggc tggaaggccg tgctgtacgg ctcgagcggc gcctacctgg
     2941 accgcgtcat cgcggcaggc ttcgacggcg tctacctgga catcatagac gcgttcgaat
     3001 acttcgagga ggcgctcggg ctctagcgcg gcgaaaaaaa accgcggcca tgcggccgcg
     3061 gctttatcgt agcgctcggg aatcgagcct acttcacgcg ctcctggaag ctaccgtcac
     3121 gggtgtcgag cctgatcctc gtgccgatgt cgcagaacag gggcacctgg accacgcagc
     3181 cggaatcgag ggtagccggc ttggacgact ttcccgaggt gtctcccttg atgccgggct
     3241 cgcagtaggt gatagtccgc tcgatggaga tcgggagcac gacgccgacc ggcttgccct
     3301 cgtagaaggt aatctcgacg tcgaagtcgt cctcgggcgt gatgtactgg gcgttatcgc
     3361 cgaggtcatc cttggtcagc tcgtactgct cgaagctctc ctggtccatg aagacgtagt
     3421 tgtcgccgtc gatgaaggag aggcgcatct tg
//
LOCUS       DAOOLN010000048         3444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_33193_length_3444_cov_2.901446, whole genome shotgun sequence.
ACCESSION   DAOOLN010000048 DAOOLN010000000
VERSION     DAOOLN010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33193_length_3444_cov_2.901446"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PLW80_01025"
     CDS             complement(<1..587)
                     /locus_tag="PLW80_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65112.1"
                     /translation="MTGAFTLAIGERLYGLRLAGGRLGSRPVAGSEAATGRLVRLEPR
                     GPGGPVPGGAAAGVDWDAAEDLLGDAGFWAALSPEDAASLADIAGRVAAELEGGRPGA
                     EAMAAACALELVVSAARLARSYPSARRPWREPDGVWSVDDAVRYIEANYAESFTLDWF
                     VARCATNASDFSRRFKERAGCPLFEFINRQRVARA"
     gene            complement(652..1632)
                     /locus_tag="PLW80_01030"
     CDS             complement(652..1632)
                     /locus_tag="PLW80_01030"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF039236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HPB65113.1"
                     /translation="MIRNLWYAVLESKEIPKNKPVGFMRLGERMVFWRDEAGEAHCCF
                     DKCAHRGASLALGDLVDGHARCPFHGLEYDGSGRCVRIPANGRDAPVPPAFRMRSYPV
                     FEGWGWVFIWWGDGEPDPAEPPYFEDLKGFTRWGSVQDPWNNHYTRVAENQIDVAHLP
                     FVHHNTIGRGNKTLVEGPGVEWVSDRLFFQYVYNKVDDGSKPRGPNEVPVPDPSRDYK
                     IEFLMPNLWENRISEKLRVSAAFVPVDDEHTLLYLRFYPGFMPFPPLAWLIGKIGSRM
                     NLVIAHQDRRVVNTQLPKSDGTGTGENLFRGDKPIMEFRKKRQELRKALG"
     gene            1800..>2606
                     /locus_tag="PLW80_01035"
     CDS             1800..>2606
                     /locus_tag="PLW80_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 family protein"
                     /protein_id="HPB65114.1"
                     /translation="MAGTKAFDNETYIAEQASCIRERMRRFDGRLYLEFGGKLLADFH
                     AARVLPGYDPNVKIRLLKELERDAEIVLCIHAGAIEHRKIRADYGITYDADAMKLIDD
                     LGEWGLSVSAVVITRWEGQPAAKAFRTKLENRGVAVYVHEPTKGYPTDVDRIVSDEGY
                     GANPRIATTKPLVVVTGPGPGSGKMATCLSQVYHDRRIGERAMYAKFETFPVWNLPLK
                     HPVNLAYEAATLDLRDFNLIDPFHLEATGEVAVNYNRDVESFPILKGILDR"
     assembly_gap    2607..2616
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2617..3388
                     /locus_tag="PLW80_01040"
     CDS             <2617..3388
                     /locus_tag="PLW80_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1846 family protein"
                     /protein_id="HPB65115.1"
                     /translation="APDEEHLALAILDRITGEPDAYRSPTEMGVNRVGFAIVDDEAAR
                     EAARQEAARRYLRCAAEYMMGMADKAALDKADLLLKELGIGPEYRKVVPAARAAAAEA
                     RERAEADPGAAAGGVAACGAALELPSGEIVSGKNSKLLHAATAAVLNAVKALAGVPDQ
                     IHLLSPLVIESIARLKREVLGRKSASLDVDEALIALSISATLTPMVQACVDHLHELRG
                     CQMHLSHLPTPGDEAGLRKLGVNLTADPAYATKRLFVE"
BASE COUNT          550 a         1156 c         1208 g          520 t
ORIGIN      
        1 caggcgcggg cgacgcgctg gcggttgatg aactcgaaca gggggcagcc ggcgcgctcc
       61 ttgaagcggc gcgagaagtc gctcgcgttc gtggcgcacc tggcgacgaa ccagtccagg
      121 gtgaaggact cggcgtagtt ggcttcgatg tagcggaccg cgtcgtcgac cgaccagacg
      181 ccgtcgggct cgcgccaggg ccggcgggcg ctcgggtagc tgcgggccag gcgggcggcc
      241 gacacgacca gctccagcgc gcaggcggcc gccatggcct cggctcccgg ccgcccgccc
      301 tcgagctctg cggcgacgcg gccggctatg tccgcgagcg acgcggcgtc ctcgggcgac
      361 agggcggccc agaaccccgc gtcgccgagc aggtcctcgg cggcgtccca gtcgacgccg
      421 gccgccgctc ctccgggcac ggggccgccg ggcccgcgcg gctcgagccg tacgagccgg
      481 cccgtcgcgg cctcggaacc ggcgaccggg cgcgagccaa ggcggccgcc ggctaaccgg
      541 agaccgtaga gccgctcgcc gatggcgagc gtgaacgcgc ccgtcatgac gccccggccc
      601 gcggggccgg ggggcgggcc gaacggcccg gctcgggggc aggccctatc gtcaacctag
      661 agccttccgg agttcctgac gcttcttgcg gaactccatg atgggcttgt cgccgcggaa
      721 caggttctcg ccggtgcccg tgccgtcgga cttgggcagc tgcgtattga cgacgcgtcg
      781 gtcctggtgg gctatgacca ggttcatgcg gctgcctatc ttgccgatga gccaggccag
      841 cggcgggaag ggcatgaagc cgggatagaa gcgcaggtag agcagggtgt gttcgtcgtc
      901 gaccggtacg aaggcggccg acacgcggag cttctcggag atgcggtttt cccagaggtt
      961 gggcatgagg aattcgatct tatagtcgcg gctcgggtcg ggcaccggta cctcgttcgg
     1021 gccgcggggc ttggaaccgt cgtcgacctt gttgtagacg tactgaaaga acagccggtc
     1081 ggaaacccat tcgacgcccg ggccctcgac gagggtcttg ttgccgcggc ctatggtgtt
     1141 gtggtggacg aaggggaggt gcgcgacgtc tatctggttc tcggcgacgc gggtgtaatg
     1201 gttgttccac gggtcctgga cgctgcccca gcgcgtgaag cccttgaggt cctcgaagta
     1261 cggaggctcg gccgggtcgg gctcgccgtc gccccaccag atgaacaccc agccccagcc
     1321 ttcgaacacc gggtaggagc gcatcctgaa cgccggcggc accggcgcgt ccctgccgtt
     1381 ggcgggtatc ctgacgcaac ggcccgagcc gtcgtactcg agtccgtgga aggggcagcg
     1441 ggcgtggccg tcgacgaggt cgccgagcgc gagggacgcg ccccggtgcg cgcacttgtc
     1501 gaagcagcaa tgggcctcgc cggcttcgtc gcgccagaag accatgcgct ccccgaggcg
     1561 catgaagccg accggcttgt tcttaggaat ttccttggat tccagcaccg cgtaccatag
     1621 gtttctgatc atcggccgtc tcctgtaggt tcagctcatg gtgccggtac tataatccgg
     1681 cgaattcggg catgcaagga cgaaatcgca ggccgcgtgt tctggcgcct cgcggccgtt
     1741 atctggcgtt ccggctacgt tccggtgtag actgtcgtcc cgtcagggag gctccatcga
     1801 tggcgggaac caaggcgttc gacaacgaga catacatagc cgagcaggcg tcgtgcatcc
     1861 gggagcgcat gcgccgcttc gacgggcgcc tgtacctgga gttcggcggt aagctcctgg
     1921 ccgacttcca cgcggcgcgg gtactgcccg gctacgaccc gaatgtcaag atacggcttc
     1981 tcaaggaact ggagcgcgac gcggagatcg tgctgtgcat ccacgccggg gcgatcgagc
     2041 accgcaagat ccgcgccgac tatggcatca cctacgatgc cgacgcgatg aagctgatcg
     2101 acgacctggg cgagtggggc ctatcggtct cggcggtcgt gatcacgcgc tgggagggtc
     2161 agccggcggc caaggccttc cgcaccaagc tcgagaaccg cggcgtggcc gtctatgtgc
     2221 acgagccgac caagggctac ccgaccgacg tggaccgtat cgtctccgac gagggctacg
     2281 gcgccaaccc gcggatagcg acgacgaagc ccctcgtcgt ggtgaccggc ccggggccgg
     2341 gctcgggcaa gatggccacc tgcctgtcgc aggtctacca cgaccgccgc atcggagagc
     2401 gggccatgta cgccaagttc gagaccttcc cggtctggaa cctgccgctc aagcacccag
     2461 tcaacctggc ctacgaggcg gcgaccctcg acctacggga tttcaacttg atcgacccct
     2521 tccacctcga ggcgaccggc gaggtagcgg tcaactacaa ccgcgacgtc gagtcgttcc
     2581 cgatactcaa gggcatcctg gaccgcnnnn nnnnnncgca cccgatgagg agcacctggc
     2641 gctcgccatc ctggaccgca tcaccggcga gccggacgcc taccgctcgc ctaccgaaat
     2701 gggggtcaac cgcgtcggct tcgcgatcgt cgacgacgag gcggcccggg aggccgccag
     2761 gcaggaggcg gcgcgccgtt acctgcgttg cgccgccgag tacatgatgg gcatggccga
     2821 caaggcggcc ctcgacaagg ccgacctgct cctcaaggag ctgggcatcg ggccggagta
     2881 ccggaaggtg gtgccggccg ccagggccgc cgcggccgag gcccgcgagc gcgccgaggc
     2941 cgatcccggt gccgcggccg gaggcgtcgc cgcctgcggg gcggccctcg agctgccttc
     3001 cggggagatc gtcagcggca agaactccaa gctgctccac gcagcgacgg ccgccgtgct
     3061 caacgccgtc aaggcgctgg cgggcgtgcc cgatcagatc catctcctgt cgccgctagt
     3121 catcgagtcg atagccaggc tcaagcgcga ggtgctcggc cgcaagagcg ccagcctgga
     3181 tgtggacgag gcgctcatag cgctgtcgat cagtgccacc ctgacgccaa tggtccaggc
     3241 ctgcgtcgac cacctgcacg agctgcgcgg ttgccagatg cacttgtccc acctgccgac
     3301 gcccggcgac gaggcgggcc tgcgtaagct cggcgtcaac ctgaccgccg acccggccta
     3361 cgcgacgaag cggctgttcg tagaataggc gcgccgtcat agtaacggcg tcgcgccgtc
     3421 atagtaacgg cgtcgcgccg tcat
//
LOCUS       DAOOLN010000049         3430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_33372_length_3430_cov_2.296889, whole genome shotgun sequence.
ACCESSION   DAOOLN010000049 DAOOLN010000000
VERSION     DAOOLN010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33372_length_3430_cov_2.296889"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1529
                     /gene="dnaX"
                     /locus_tag="PLW80_01045"
     CDS             <1..1529
                     /gene="dnaX"
                     /locus_tag="PLW80_01045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPB65116.1"
                     /translation="SLNCERGPTDKPCGACDSCLSIAKGSSLDVIEIDGASNTSVENV
                     RQIKDEVLFPPNSSKYKIYIIDEVHMLSNSAFNALLKTIEEPPPYIVFIFATTELHKV
                     PATIKSRCQQFGFRLFSLDVIKGLLRSAADDMGLEADDDALLWIAREATGSMRDAYTL
                     FDQVASFSDGRITAAKIRDKLGLVGSERMGALMSLCVSGDRKGALETLDAILDSGVSV
                     EQFASDAVSYIRSLLLVASGIERESLLGAPRSSFPAALTEALDAPRLERALSGLFDLY
                     RDMRYSVNPRWELELAVSRLCAIRDYVSSAELSRTIRGMKAAIVEGRAPGAAAVGAAL
                     REASAVSPRIEAAAAERPDPAAAGAEPPAENPGSATPGLDVDGIDTVALRASLLAAVG
                     KNRVVLSTCLERSGDWVVDGDKLVIHCLNGYDAGAVLADAGLLAKKALDITGRPLRIE
                     PRAAEPAPAPTPPARSGDDEDSDDGDDGPVDPVGIVEKVFRGRRVSAPASNERSESWT
                     "
     gene            1520..1768
                     /locus_tag="PLW80_01050"
     CDS             1520..1768
                     /locus_tag="PLW80_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPB65117.1"
                     /translation="MDMSDIMKMLKDPQALQRQALEAQERMAAISAVGSAGGGMVRVT
                     LSGTMELLNVEIAPEAIDPSDPAMLQDLIKAAHNDASY"
     gene            1831..2418
                     /gene="recR"
                     /locus_tag="PLW80_01055"
     CDS             1831..2418
                     /gene="recR"
                     /locus_tag="PLW80_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPB65118.1"
                     /translation="MKALEDLVGILARLPGIGRKSAARIAFHILRSDDQYVALLAERI
                     GSLKERVRFCSVCGSYAEDDPCEVCSSPMRDRSLICVVEQAQDVLTIEASREYRGVYH
                     VLGALISPLDGIGPDALRLDTLVARVRDQGVAEVVIATNPTVEGDTTALYIKKVLEGC
                     GARVTRLATGIPVGGDLEYADKLTLARSFRGRVAL"
     gene            complement(2415..>3430)
                     /locus_tag="PLW80_01060"
     CDS             complement(2415..>3430)
                     /locus_tag="PLW80_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65119.1"
                     /translation="RRAEAERASYLLGQFYDQATEDEGADFITASVATEERRLKERRE
                     ESEKNFFDLSKIEFDEEDVRRIEIPAGLERDEDLVLAWCHKYWLRVDDPAFERIVWLY
                     SKKYGTKHYEVFKAIKTGRHRKYQDDDILTMVATTIATRYSYTVQGDLYMQSAWRRVK
                     ASMYGQVPPRRPAQTETTEREPAQRKPRSAKPRRERKAATPLVTQTRPKPDERPKLRP
                     AAAPAKAIAIPDDRRPTGSISDRIRRLSGRAYDVYRDIFLAKVRVSIREELGKRRTKP
                     GISREALNEAENLVYEFMDRNYANAYMDWESSEHRKRVAALGFSLDGLDEVVEACYRR
                     LGS"
BASE COUNT          564 a         1078 c         1131 g          657 t
ORIGIN      
        1 gatcgcttaa ctgcgagcgc ggccctaccg acaagccctg cggagcctgt gactcgtgcc
       61 tgtccatcgc caagggctcc tcgctcgacg tgatagagat tgacggagca tcgaacacct
      121 cggtggagaa tgtacggcag atcaaggatg aggtactgtt tcctcccaat tcgtcgaagt
      181 acaagatcta catcatcgac gaagtacaca tgctgtccaa tagcgccttt aacgcgctcc
      241 tcaagaccat agaggagccg ccgccctata tcgtcttcat cttcgcgact accgagcttc
      301 acaaggtccc cgctacgatc aagagtcgtt gccagcagtt cggctttagg ctgttctcgc
      361 tggacgtgat caaagggctg ttgcggagcg ccgcggacga tatggggctc gaggccgatg
      421 acgacgcgct cctatggata gcccgcgagg ccacgggctc gatgcgcgat gcatatacgc
      481 tgttcgacca ggtagcctcg ttctccgacg ggcgtattac cgccgccaag atccgcgata
      541 agctcggcct cgtcggtagc gagcgcatgg gcgccctgat gagcctatgc gtgtccgggg
      601 acaggaaggg cgccctcgag accctcgacg ccatcctcga ctcgggcgtg tcggtggagc
      661 agttcgcttc cgacgccgtc tcctacatcc gatccttgct gctcgtcgcg agtgggatcg
      721 agcgggagag cctcctggga gcccccagat cgtcgttccc ggccgccttg accgaggcgc
      781 tcgacgcgcc gcgcctggag cgagcgctgt ccggcctgtt cgacctgtac cgggatatgc
      841 gctatagcgt caacccgcgc tgggagctcg agctggccgt ctcgaggctc tgcgccattc
      901 gcgactacgt ttcgtcggcc gagctgtcca ggaccattcg cggcatgaag gccgccatcg
      961 tggagggccg ggcgcccggc gcggcggccg taggggccgc gcttcgcgaa gcgtcggccg
     1021 tttcgccccg catcgaggcc gcggcggcgg agcgacccga tccagccgcc gccggcgccg
     1081 agcctccggc ggagaatccg gggtcggcca cgccgggttt ggacgtcgac ggcatcgata
     1141 ccgtggcgct cagggcttcg ctactggcgg ccgtcggcaa gaacagggtg gtcctgtcta
     1201 cctgcctgga gcgttccggg gactgggtag tggacggaga caagctggtc atccattgcc
     1261 tgaacggtta cgacgccggc gccgtgcttg ccgacgccgg cttgttggcc aaaaaggcgc
     1321 tggacattac cggtcggccc ctgcgtatcg agcctcgagc ggcagagccc gccccggcgc
     1381 cgacgcctcc agcgaggtcg ggcgacgacg aagactcgga cgacggagac gacgggcctg
     1441 tcgatccggt aggcatcgtc gagaaggttt tccgcggccg gcgcgtctcg gcgccggcga
     1501 gcaacgaacg gagcgaatca tggacatgag cgatatcatg aagatgctga aggatccgca
     1561 ggccctgcag cgccaggcct tggaggccca ggaacgcatg gccgccatca gcgcagtcgg
     1621 ttcggctggg ggcgggatgg tccgcgtcac gctgagcggg accatggaac tcctgaacgt
     1681 cgagatagcg cccgaggcga tagacccatc cgacccggct atgctccagg acctgatcaa
     1741 ggcagcccac aacgacgcgt cctactgagt caaggaagcg ctacaggccg acgtcgcccg
     1801 taacctcggc tcgatgccgg gcctgggccg gtgaaggcgc tcgaggacct cgtcggtatc
     1861 ctggccaggt tgccgggcat aggtcggaag agcgccgcca ggatcgcctt tcatatactc
     1921 aggtctgacg accagtacgt tgcgctcctg gcggaacgta tcggttcgct taaggaacga
     1981 gtgcgcttct gctccgtctg cggttcgtac gccgaggacg acccctgtga ggtctgctcc
     2041 tcgccgatgc gcgaccgaag cctgatctgc gtggtggagc aggcgcagga cgtgctgacc
     2101 atcgaggcct cgcgcgagta ccggggcgtc taccatgtgc tcggcgccct gatctcgccg
     2161 ctggacggca taggccccga tgcgctcagg ctcgatacgc tggtcgccag ggtgcgcgac
     2221 cagggtgttg ccgaggtggt catagcgacc aatccgaccg tggagggcga tacgacggcc
     2281 ctctacataa agaaggtgct cgagggctgc ggggcgaggg tgacgcgcct ggcgaccgga
     2341 atcccggtcg gcggcgactt ggaatatgcc gacaagctga cattggccag gtcgttccgc
     2401 ggccgcgtcg cgctctagga gcccagcctc cggtaacagg cctcgacgac ctcgtcgagg
     2461 ccgtcgagcg agaagccgag cgctgcaacg cgcttccgat gctctgacga ctcccagtcc
     2521 atgtacgcgt tggcgtagtt cctgtccatg aactcataga cgaggttttc tgcctcgttc
     2581 aacgcttcac gcgatatccc cggcttggtt cgacgtttac cgagttcctc gcgtatgctt
     2641 acgcgtacct tagcgaggaa aatatcccga tatacgtcat aggcgcgacc agaaagccgc
     2701 ctgatcctgt cgctgatgga gcccgtcggc cttcggtcgt ccggtatggc tatggccttg
     2761 gcgggagcgg ccgccggccg aagtttcggc cgctcgtcgg gcttcggtcg ggtctgggtg
     2821 acgaggggcg tcgccgcctt acgctctcgc ctgggctttg ccgaccgcgg cttacgctgg
     2881 gcgggctctc tctcggtggt ttctgtttgc gccggcctac gcggcgggac ctgtccgtac
     2941 atggacgcct tgacgcgacg ccatgctgac tgcatgtaca ggtcgccttg aacggtgtaa
     3001 ctgtagcggg ttgcgatggt cgtggcgacc atggtcagta tatcgtcgtc ttggtacttc
     3061 cggtgacggc cggtcttgat ggctttgaac acctcgtagt gcttcgtacc atatttcttg
     3121 gagtagagcc atacgatgcg ctcgaaggcc gggtcgtcga ccctgagcca atacttgtgg
     3181 caccaggcga gcacgagatc ctcgtcgcgt tcgaggccgg ccgggatctc gatgcgccga
     3241 acgtcctctt cgtcgaattc tatcttggat aggtcgaaga agttcttctc gctctcctcg
     3301 cgtcgctctt tgagcctgcg ttcctcggtg gcgacgctcg cggttatgaa gtcggcgcct
     3361 tcgtcctcgg tcgcctggtc gtagaactgt ccgagtaggt aggacgctcg ctcggcttcg
     3421 gcgcgccggt
//
LOCUS       DAOOLN010000050         3421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_33485_length_3421_cov_3.097148, whole genome shotgun sequence.
ACCESSION   DAOOLN010000050 DAOOLN010000000
VERSION     DAOOLN010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33485_length_3421_cov_3.097148"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..330)
                     /locus_tag="PLW80_01065"
     CDS             complement(52..330)
                     /locus_tag="PLW80_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HPB65120.1"
                     /translation="MTIDRLNPLDPIQPKKPSGAARAERNSGSDSVSLSSEAVEKAEL
                     FKALELAKAAPEIRMDRVAELKAKIDDPDYLNDAILSATADRILDQLL"
     gene            complement(383..1063)
                     /gene="rsmI"
                     /locus_tag="PLW80_01070"
     CDS             complement(383..1063)
                     /gene="rsmI"
                     /locus_tag="PLW80_01070"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPB65121.1"
                     /translation="MATLYMVATPIGNLEDITLRAVRTLGEVDAIACEDTRHTLRLLN
                     HLGVKKQLVACYAYEEEKGSARILALLEQGKDVAYCSDAGTPCLSDPGILAAARAREA
                     GHDVVPIPGPSAFAALVSASGIFYKTVAFDGFPSPKPGRRRSRVAELMSRDEAFVMYE
                     SPHRIVKLLTDIADIDAERRLCVGREMTKLHEEFLVGSAAEVRDLLAARPEVKGEIAL
                     LVSGAKKG"
     gene            1126..1551
                     /locus_tag="PLW80_01075"
     CDS             1126..1551
                     /locus_tag="PLW80_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65122.1"
                     /translation="MNDLVFAALLASIGAFLLWLFWALVYSPYAARKERASPRFVPDA
                     RGEMREKGVGARSCPVCGERLGPGALVKSKVYKSSGSDKVMYIYGCPYCWPENSEYRR
                     VCPVCEKVVPRDGYLLARYFERPERRHVHVLGCSGCRRG"
     gene            1750..3117
                     /locus_tag="PLW80_01080"
     CDS             1750..3117
                     /locus_tag="PLW80_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65123.1"
                     /translation="MRAISVPLVIVTAFALGPGVAAFAQGRPEGPGFEPLPLSSPAYA
                     AVDALYLATGAGTASNARPWSRSEAMQILGRIDRSSLGAQLSGLYDAAERAIGAGRRF
                     GTPDGFSLGGGLDLAAEAYYHANPDDFYLESDWAHGFESREPLARLRLDFAIDELMYV
                     YCDLQYGRNRISYLDEVSNMPDAGVGAVIGERTAVDDSADRAERGAYVERSYSFGSGL
                     ISNVVLHSYDFDFEWPKRAVATIGGDRWTASLARDSISWGNGRSGNFVLDGHADFNDY
                     ARVTAWSDRFKYDWVNVFLPTETGTAEAPDQAFRVFMGHRLEFRALDSLTLALSEDVM
                     YRNDVFDPRYLNPAFIYHNLNNRSMFNAIAHLELDWAPAPGWKLYAQYALDQGRAPNE
                     SSAQANAMAYLGGLERGFALGRGILTSSIELAWTDPLLYRRDGVDFIVVRRYFCHGDP
                     GATAT"
     gene            3114..>3421
                     /locus_tag="PLW80_01085"
     CDS             3114..>3421
                     /locus_tag="PLW80_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65124.1"
                     /translation="MSHLDYLGYPYGGDVQLARADLEYLAPGVGTFSAYATAMIRGRM
                     SFFTSHNLGGDNGGMANYEGSTPSGDAVDERLVVGLYARVALPKPAIAAEASAWLRVD
                     "
BASE COUNT          573 a         1139 c         1112 g          597 t
ORIGIN      
        1 aaaaggacgg aagcgcaagc ctccgccctc gtttggacac catgaagacg ctcagagcaa
       61 ctggtcgagt atgcgatccg ccgtggcgct caggatcgcg tcgttcaggt agtcggggtc
      121 gtcgatcttg gccttgagct cagcgacgcg atccatcctg atctccggcg ccgccttggc
      181 cagctcgaga gccttgaaga gctcggcctt ctcgacggcc tccgaggaca ggctaacgga
      241 atcgctgcca gaattcctct cggcgcgagc ggcgcccgag ggtttctttg gctggatcgg
      301 atccagcggg ttcagcctat caattgtcat cggtggcccc tgcctttcac atactcaata
      361 tcggcaccgt aaggcgttta gtttacccct ttttcgcccc ggacacaagt agcgctatct
      421 cgcccttgac ctcgggccgc gccgcgagca ggtcccggac ctcggccgcc gatccgacca
      481 ggaattcctc gtggagcttg gtcatctcgc ggccgacgca caatcgcctc tcggcgtcaa
      541 tatcggcaat atccgtaagg agcttcacta tgcggtgcgg cgattcgtac atgacgaagg
      601 cctcgtcgcg cgacatgagc tcggcgaccc tggaacggcg ccggccgggc ttcggcgagg
      661 ggaagccgtc aaaggcgaca gtcttataga agatgcccga cgccgagacc agcgcggcga
      721 aggcgctcgg gccggggata ggcacgacgt cgtggccggc ctcgcgggcg cgggctgccg
      781 ccaggatgcc tgggtcggag aggcagggcg tgccggcgtc ggagcagtag gccacgtcct
      841 tgccctgctc caggagagcg aggatgcggg cggagccctt ctcctcctcg tacgcgtagc
      901 aggcgacgag ctgcttcttg acgcccaggt ggttgagcag cctgagcgta tgcctggtat
      961 cctcgcaggc tatcgcgtcg acctcgccga gcgtcctgac ggcgcgcagg gttatgtcct
     1021 cgaggttgcc tatgggggtc gcgaccatgt acagggttgc catcgcccca gtatcgccgc
     1081 aatcggctcg ggcagtcaac gtcgcgccat gctatactgg cccgcatgaa cgacctcgta
     1141 ttcgcggccc tgctcgcgtc gatcggcgcc ttcctcctct ggctgttctg ggccctcgtc
     1201 tacagccctt acgccgcccg caaggagcgc gcctcgcctc gcttcgtgcc cgacgcccgc
     1261 ggcgagatgc gcgagaaggg cgtaggcgcg cggagctgcc cggtctgcgg cgagcggctc
     1321 ggccccggcg ccctggtcaa gtcgaaagtc tacaagagtt ccggcagcga caaggtgatg
     1381 tacatctacg gctgcccgta ctgctggccg gagaacagcg agtaccgtcg agtctgcccg
     1441 gtatgcgaaa aagtcgtgcc gcgcgacggc tacctgctag cgcgctactt cgagcgcccc
     1501 gagcgtcgcc atgtccacgt gctcggctgc tccggctgcc gaagagggtg atccgactgg
     1561 aaccagcgaa gagcatacgt gtgaaggctt gaaccacgga aacacggaga cacagaggaa
     1621 taagacaagt tcttatcatc ctctccgtgc ctctgcggca aagcccaaag agttccttcc
     1681 aatccgacta tgaactcgcg ggctggcgga gaggcggcgc gagcggtaga attcgaccgg
     1741 aggcatacga tgcgcgccat atccgttcct ctcgtcatcg tcaccgcttt cgcgctcggc
     1801 ccgggggtag cggccttcgc ccagggccga ccggaaggtc ccgggttcga gcccctgccg
     1861 ctgtcgtcgc cggcctatgc cgccgtggac gcgctctacc tggctaccgg cgccgggacc
     1921 gcgtcgaacg cgaggccctg gtccaggtcg gaagccatgc agatcctcgg acggatcgac
     1981 cgatcatcgc tcggggccca gctctccggg ctctacgacg cggccgagcg ggcgataggc
     2041 gctggacggc gcttcggtac gcctgacggg ttcagcctgg ggggcgggct cgacctggcg
     2101 gccgaggcct actaccacgc gaatccggac gacttctatc tcgagtccga ctgggcccac
     2161 ggcttcgagt cgagggagcc gctggccagg ctcaggctcg acttcgccat cgacgaactc
     2221 atgtacgtct actgcgacct gcagtacggc cggaaccgga tcagctacct ggacgaggta
     2281 tccaacatgc ccgatgccgg cgtcggcgcc gtgatcggcg agaggacggc cgtggacgac
     2341 tcggcggatc gagcggagcg aggcgcctac gttgagcgtt cgtatagttt cggtagcggc
     2401 ctgatctcga acgtcgttct ccatagctac gacttcgact tcgagtggcc caagcgggcc
     2461 gtggcgacga taggcggcga tcgatggacc gcgtcgctcg cgagggactc gatatcgtgg
     2521 ggaaacggcc gttccggcaa cttcgtgctc gacggccacg ccgacttcaa cgactacgcg
     2581 cgcgtcaccg cttggtccga ccgcttcaag tacgactggg tcaacgtgtt cctgccgacg
     2641 gagacgggta cggccgaggc cccggaccaa gcgttccggg tattcatggg gcaccggctt
     2701 gaattcaggg cgctggatag cctgacgctc gcgctgtccg aggacgtgat gtaccgtaac
     2761 gacgtcttcg acccgcgcta cctgaacccc gccttcatct accacaacct gaacaatcgc
     2821 tcgatgttca acgccatcgc tcacctggag ctggactggg cccccgcgcc cggctggaag
     2881 ctctacgccc agtacgccct cgaccagggc cgcgctccga acgagtcgtc ggcccaggcc
     2941 aacgccatgg cctacctcgg cggcctcgag cggggcttcg cgctcggccg gggcatactg
     3001 acctcgtcca tcgagctggc ctggaccgat cccctgctct accgccgcga cggcgtcgac
     3061 ttcatcgtcg tccgcaggta cttctgccac ggcgatccgg gggctacggc tacgtgagcc
     3121 acctcgacta cctgggctat ccgtacggcg gcgatgtcca gctggcgcga gccgacctcg
     3181 agtacttggc gccgggcgtc gggaccttca gcgcctacgc gacggccatg atacgcggcc
     3241 ggatgagctt cttcacctcg cacaacctgg gcggcgacaa cggcggaatg gcgaactacg
     3301 aaggctcgac gccgtcaggc gacgcggtcg acgagcgcct cgtcgtcggt ttgtacgcta
     3361 gggtagcgct accgaagccg gccatcgccg ccgaggctag cgcctggcta cgggtggatt
     3421 g
//
LOCUS       DAOOLN010000051         3391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_33891_length_3391_cov_2.566547, whole genome shotgun sequence.
ACCESSION   DAOOLN010000051 DAOOLN010000000
VERSION     DAOOLN010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33891_length_3391_cov_2.566547"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PLW80_01090"
     CDS             complement(<1..228)
                     /locus_tag="PLW80_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65125.1"
                     /translation="MASNNDKHVRPESSDRRPRGNSFIYIGTVALLIITVVAFVFVPA
                     MGSMGGSGGELNFGSWNGKPIAFAQGGYFASQ"
     gene            complement(375..2555)
                     /locus_tag="PLW80_01095"
     CDS             complement(375..2555)
                     /locus_tag="PLW80_01095"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB65126.1"
                     /translation="MTTNDTRFQAVDSANARAWDILSAQPQSALEEAERALATASEAG
                     YAKGVADAHLNIGWSMYYLTHLPEAYRAFLEVHREYLALGDVLGVCKTLNALGVYHYS
                     IFRLEKAVDYFTQSLEVAKANGIEDRELIVGINIGELCLLLGNPQDALEYLIPAYVRM
                     IGVLPPSNVADCLRNIGKAFLQLDNPALAAEYIRRSYSLAKDVGESIMATDSVETLAD
                     VAIESGEYDEAERLVREGLELVDVTGNSGQRVGLLIVEGTLLNARGRHGEALGPLKRA
                     EELSESLNLKSRLFKVHEQMSEVYRALGDYERALDYYKKFSDFRQLVQHEDTANKLRG
                     FQAQVDIERAQQEAEIYRLRNIDLKEKTEALENINRQITSISRIGQRITASLDYGTVV
                     QTLYDCLKPFIDIDMFGIALSDPGRGQLVYKRYYEDGVRKSDWRINLATDSSFTVWAF
                     RNNKPVLITDKENEYGQYLTRRPSSKGHPSQSIVCMPLSIEEKTIGVMTIQNYEPRAY
                     APSDLSFLQALAPYVSIAVENALIHDRLEVLNRALSDEKRRLERATLKISHLANHDTL
                     TGLPNRRLLVELMDKSVETARRTGSKIGVVFMDLDDFKPINDQFGHTAGDSALVAMAD
                     RIRGLVRASDIVARIGGDEFLAIITNIQHRDDVERVARKVIEECGTPLTFSGASWKVG
                     VSIGIALFPDDGESIEDLVNRADSAMYRVKHMSKNAYSFWGDSA"
     gene            2704..3231
                     /locus_tag="PLW80_01100"
     CDS             2704..3231
                     /locus_tag="PLW80_01100"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPB65127.1"
                     /translation="MSIVTRTGDEGMTGLWSGERVRKEDPRVEAYGTVDELDSVIGEA
                     KHWLKRVDCRRIAEELQRDLYRVAGQLATRTGSFSKPMTDADEVRLASYVGAFEDEIP
                     LTGFVVPGSTLQSAKLDVARTVCRRAERRIVALSRDADVPGQVIRYMNRLSDLLFMMA
                     RAEEASDGGVRYVRD"
BASE COUNT          571 a         1015 c         1126 g          679 t
ORIGIN      
        1 ctgcgaagcg aagtagcctc cctgcgcgaa ggctatcggc ttgccgttcc aggagccgaa
       61 gttgagctct ccgcccgagc cgcccatgct gcccatcgcc gggacgaaga cgaacgcgac
      121 gaccgtgatg atgaggagcg cgacggtgcc tatgtagatg aatgaattgc ctcgagggcg
      181 acggtcgctc gattcgggcc tgacgtgctt gtcgttgtta gatgccatcg aaaccttcct
      241 ggaacatatg atgggtggac ggtcgctcgg ccggtattga cggtactctc gttagcaaca
      301 atccctcgac gtaaagcgcg gaaaaacata gcacggggct atggcagtgt caatcgagtc
      361 cgatccgggc gtttctaggc gctgtcaccc cagaacgagt aggcgttctt gctcatatgc
      421 ttgacccggt acatggccga gtcggcgcgg ttgacgaggt cctcgatgga ctcaccatca
      481 tcggggaaga gcgctatacc tatcgatacg cccaccttcc aggaggcgcc ggagaaggtc
      541 aggggggttc cgcactcctc tatgaccttg cgggcgactc gctccacgtc gtcgcggtgc
      601 tggatgttcg tgatgatggc caggaactca tcgccgccga tgcgcgccac gatgtcggag
      661 gcccgcacga ggccgcggat gcggtcggcc atcgcgacga gggcgctgtc tccggccgta
      721 tggccgaact ggtcgttgat cggcttgaag tcgtccaggt ccatgaacac gacgccgatc
      781 ttgcttccgg tgcgcctggc cgtctcaacg ctcttatcca tcagttctac gaggaggcgc
      841 cggttcggga gccccgtcag cgtgtcgtgg ttagccaggt ggctgatctt gagcgtagcg
      901 cgctcaagcc gccgcttctc gtccgagagg gcgcggttca ggacctccag gcggtcgtgg
      961 atcagggcgt tctcgacggc gatgctgaca tacggagcca gggcctggag gaaggacagg
     1021 tcgctcggag cgtacgcgcg cggttcgtag ttctggatgg tcatcacgcc gatggtcttt
     1081 tcctcgatcg acagcggcat acagacgatc gactgcgagg ggtgcccctt gctcgatggc
     1141 cgcctggtca ggtactgccc gtactcgttc tccttgtcgg tgatcaggac cggcttgttg
     1201 ttcctgaacg cccagacggt gaagctcgaa tccgtcgcca ggttgatcct ccagtcgctt
     1261 ttacgcacgc cgtcctcgta gtagcgctta tagacgagct ggccgcgccc ggggtcggag
     1321 agggctatgc cgaacatgtc tatgtcgatg aagggcttga ggcagtcata gagggtctgc
     1381 accaccgtac cgtagtcgag actcgccgtg atgcgttggc ctattcgcga gatagaggtg
     1441 atctggcgat tgatgttctc gagcgcctcg gtcttctcct tgaggtcgat gttgcgcagg
     1501 cggtagatct cggcttcctg ctgggcgcgt tcgatgtcga cctgggcttg gaaaccgcgc
     1561 agcttgttcg ccgtgtcctc gtgctggacc aactgcctga agtcggagaa tttcttgtag
     1621 tagtcgaggg cccgctcgta gtcgcctagg gcgcggtaga cttccgacat ctgctcgtgg
     1681 accttgaaca gcctgctctt gaggttgagc gactcgctca gctcctcggc ccgcttgagc
     1741 ggcccgagcg cttcgccatg gcggcccctg gcgttcaaga gcgtgccctc gacgatgagc
     1801 aggccgacgc gctggcccga gttgccggta acatcgacca gctcgaggcc ttcgcggacc
     1861 aggcgctccg cctcgtcgta ctcgccgctc tcgatggcca catcggccag ggtctcaacg
     1921 ctgtcggtcg ccatgatgga ctcgcctacg tccttggcga ggctgtacga gcggcgtatg
     1981 tactcggccg ccagggccgg gttgtcaagc tgcaggaagg ccttgccgat gttgcgcagg
     2041 cagtcggcga cgttgctcgg cggcaacacg cctatcatcc tgacgtaggc gggtatcagg
     2101 tattcgaggg cgtcctgcgg attgccgagc aacaggcaga gctcgccgat gtttatgccg
     2161 acgatgagtt cccggtcctc gatgccgttc gccttggcta cctcgaggct ctgcgtgaaa
     2221 tagtccacgg ccttctcgag gcggaaaatc gagtagtggt agacgcccaa agcgttcagc
     2281 gtcttgcaga cgccgagtac gtcgcctagg gcgaggtact cgcgatggac ctccaggaag
     2341 gcgcgatagg cctcgggcag gtgcgtcagg tagtacatgg accagccgat attgaggtgg
     2401 gcgtcggcga cacccttggc gtaaccggcc tcggacgccg tcgccaaagc ccgctcggct
     2461 tcctcgaggg ccgactgggg ctgggcgctc aagatatccc aggcgcgagc gttggctgag
     2521 tcgacggctt ggaagcgggt atcgttcgtg gtcaatacgt gcacctattc aagaatgtta
     2581 ctatggatcg ggcgattgta tccagatcat tgtagcacga gttggcgccg ctgagcgtgc
     2641 tatatatgct actatacgcg cgccgccaaa cgcggcgcga cggagacggg gcgaggagcg
     2701 ttcatgagca tcgttacgag gaccggggac gagggaatga ccgggttatg gtcgggcgag
     2761 cgggtccgca aggaggatcc ccgcgtcgag gcgtatggca ccgtggacga gctggactcg
     2821 gtcatcggcg aggccaagca ctggctcaag cgcgtcgact gcaggcgtat cgccgaggag
     2881 ctgcaacgcg atctgtaccg ggtagccggg cagctggcca ctcgtaccgg ctctttctcc
     2941 aagcccatga cggacgcgga tgaggtcagg ctggcatcct acgtcggggc cttcgaggac
     3001 gagataccgc tgaccggctt cgtcgtaccc ggctcgacgc tccagtcggc caagctcgac
     3061 gtggccagga ccgtctgcag gcgggcggag cggcgcatcg tcgcgctgtc ccgcgacgcg
     3121 gacgtgcccg gccaggttat ccgctacatg aaccggctgt cggacctgct gttcatgatg
     3181 gcccgcgccg aggaggcctc ggacggcggc gtccggtatg tccgcgactg aggtcgagcc
     3241 cgctagtcga tcaggtcgtc gtcgccctcg aggaccaggt cgccgtcctc ccacttccgc
     3301 ttgtagtagg cgatgaaggc cttcgtggcc ttctcggcct cgtcgcgcct gaccgtgccc
     3361 atgatgtcgt actcctcgag caccatggtc c
//
LOCUS       DAOOLN010000052         3382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_34013_length_3382_cov_2.770063, whole genome shotgun sequence.
ACCESSION   DAOOLN010000052 DAOOLN010000000
VERSION     DAOOLN010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34013_length_3382_cov_2.770063"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLW80_01105"
     CDS             complement(<1..259)
                     /locus_tag="PLW80_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HPB65128.1"
                     /translation="MAPRTAAELAAVLDGHKEDGKLLAGGTDLIPGIRNGVMKPKVVI
                     DLKKYSGADRLSYGADGLSIGPAVTINDILRCKEARERYPVL"
     gene            486..1439
                     /locus_tag="PLW80_01110"
     CDS             486..1439
                     /locus_tag="PLW80_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPB65129.1"
                     /translation="MVSFPIDTEGGPQVVLELIYRLKVKDVMMRDVITASASSSLRDI
                     QLAMRDRGITGVPIVDGDRLLGIVSIGDVIQALDGGWIHELASERMSTSLIVLEDDMP
                     LSFAITYFNRYGYRRFPVLDKNASLVGIVTAADVLRALLIEMNREVEKLEEGLAGRAA
                     AARDAAATATAPGGEAGLTMSFVSRRFDFENAGKASAELKKALRGLGVDAASIRRAAI
                     VSYELEMNQVIHSDGGVMEFCATQDSVRIVARDEGPGIADVEAALTEGYSTANEWIRS
                     LGFGAGLGLPNAKRSSDEFELSSRVGAGTTVLAVVKYQERS"
     gene            1436..1768
                     /locus_tag="PLW80_01115"
     CDS             1436..1768
                     /locus_tag="PLW80_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65130.1"
                     /translation="MKVSDLSTRLGFEAIYAPAGDADVNDGYASDLLSDVMAKAGDGS
                     VLITIQAHKNTVAVASLIGIPAVIICNDRPVPSDMIEAAEAEGVALLRTPKTQFEVAG
                     TLYPELRG"
     gene            1789..2523
                     /locus_tag="PLW80_01120"
     CDS             1789..2523
                     /locus_tag="PLW80_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPB65131.1"
                     /translation="MFVACDFHNHSCLSPCGSLEQSPALLARLARKRGLGLVALTDHN
                     SALNAPAFAECAAREGVAALYGMEATSAEEVHVLCLFAEVGAALDFGRFIEARLPLLP
                     YDPGKLGDQVVVDADENVLDLPELYFGAALELDYGELCESAAERGAIVIPAHIDRPMF
                     GAISQLGFLPDGPYAAVEAVRPLPDGAARGYTVITGSDAHYPEHVARRPFGLELADGW
                     ASGGKVDLEAVRVALAEGRVRLPFAT"
     gene            2535..>3382
                     /locus_tag="PLW80_01125"
     CDS             2535..>3382
                     /locus_tag="PLW80_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65132.1"
                     /translation="MMARARGGAVAVIAAALLSLAPIVGRAAALGAVDLDDATVLAAP
                     PPRVAGSEADYTRVLVDDEASLSDRLLVHVEGPKGLPLTAFTAEPDRTLFSRLPESGR
                     RGVWNALREEPLASSTLWLCFRLDNRSPCAAWALVVEGAGFKPLAMVIVGGDGGIQHY
                     GVESLDALRMGTSLADLSAYALNVPPESERTVYLRLSEGSLFGPGFGVWSHGLFLERS
                     EARLGLMSGIAGFAVAACVFYLLLSSRGWPVPYSGGVRRADQALSYAMMLRYSFVLHT
                     QADLVDA"
BASE COUNT          515 a         1135 c         1138 g          594 t
ORIGIN      
        1 atagaaccgg atagcgctcg cgcgcttcct tgcagcgcag gatgtcgttg atggtgaccg
       61 ccgggccgat cgataagccg tcggctccgt acgacagcct gtcggcgccg ctatacttct
      121 taagatcgat gacgaccttc ggtttcatga cgccgttacg gataccgggg atgaggtcgg
      181 tcccccccgc caagagcttg ccgtcttcct tatggccatc gagcaccgcg gccagttccg
      241 ccgccgtcct aggggccaag tacctgaact cgtagaccat gtgtgctgcc tttcgagcgt
      301 attcacgaaa caaaaacccg gatcatacga accgggcttc tagaaggaaa tgctactgac
      361 ttgactaaag ttaagacacc gatccagcga ggtgtcaagt aaaatgtact ccgggcggcg
      421 gaccggccgc ctaggcggct cgtgtcggct tgtggtcgaa acggtttcat gagacaaccg
      481 ggccgatggt ctcgttccct atcgataccg agggcggccc ccaggtcgtc ctggagctca
      541 tctatcgcct caaggtcaag gacgtgatga tgcgcgacgt catcaccgcc tccgcctcgt
      601 cgagcctgcg cgacatccag ctagccatgc gggaccgcgg catcacgggg gtgcccatcg
      661 tcgacggcga caggctgctc ggcatcgtgt ccatcgggga cgtcatccag gccctcgacg
      721 gcggctggat acacgagctc gcgtccgagc gcatgagcac ctcgctcatc gtgctcgagg
      781 acgatatgcc gctgtcgttc gccattacct acttcaaccg ctacggctac cggcgctttc
      841 ccgtactcga taagaacgcc tcgctcgtcg gcatcgtcac cgcggcggat gtgctgcgcg
      901 cgctgctcat agagatgaat cgcgaggtcg agaagctcga ggaggggctg gccggccggg
      961 cggcggcggc gagggacgcc gccgctacgg cgacggcccc cgggggcgag gccggcctga
     1021 cgatgagctt cgtctcgcgg cgcttcgatt tcgagaacgc cggcaaggcc tccgccgagc
     1081 tcaagaaggc gctcaggggg ctcggcgtcg atgccgcgtc gatacgacgg gccgccatcg
     1141 tcagctacga gctggagatg aaccaggtca tccattccga cggcggcgtc atggaattct
     1201 gcgcgacgca ggactcggtc aggatagtgg cacgggatga gggaccgggg atagcggacg
     1261 tcgaggccgc cctgaccgag ggctactcca cggccaacga gtggatacgt tcgctcggct
     1321 tcggcgcggg cctgggcctg ccgaacgcca agcgatcatc ggacgaattc gagctttcat
     1381 cgagggttgg cgcggggacg accgtgctcg ccgtcgtcaa gtatcaggag cgatcatgaa
     1441 ggtaagcgat ctatcgacgc gcctcggttt cgaggccatc tacgcgccgg ccggggatgc
     1501 cgacgtcaac gacggctacg cgtccgacct cttgtcggac gtcatggcca aggccgggga
     1561 cggctcggtg ctcatcacca tacaggcgca taagaatacc gtcgcggtcg cctcgttgat
     1621 tggcatcccg gccgtcatca tctgcaacga ccgtccggtg ccgagcgaca tgatcgaggc
     1681 cgccgaggcc gagggcgtcg ccctgctcag gacgcccaag acgcagttcg aggtcgccgg
     1741 gacgctctat ccggagctgc ggggctaggt tcggcccggc cccccggcat gttcgtcgcc
     1801 tgcgacttcc ataaccactc gtgcctatcg ccgtgcggta gcctggagca gtcgccggcg
     1861 ctactggcca ggctggcccg caagcgcggt ctgggcctcg tcgcgctgac cgaccataac
     1921 agcgccttga acgctccggc cttcgccgag tgcgccgcca gggaaggcgt cgccgcgctc
     1981 tacggcatgg aggccacgag cgccgaggag gtgcatgtgc tctgcctgtt cgccgaggtg
     2041 ggagccgccc tcgatttcgg gcgcttcatc gaggcgcgct tgccgctgct gccctatgat
     2101 ccgggcaagc taggcgacca ggtggtggtc gatgcggatg agaacgtact cgacctgccc
     2161 gagttgtact tcggcgcggc cctcgagctc gattatggcg agctgtgcga gtcggccgcc
     2221 gagcgaggcg ccatcgtcat ccccgcgcat atcgaccgcc ctatgttcgg cgccatatcg
     2281 cagctcggct tcctgcccga cggcccctac gcggcggtcg aggcggtcag gccactgccc
     2341 gacggggcgg ccagaggcta cacggtcata accggctcgg acgcccatta tcccgagcac
     2401 gtggcgcggc gccccttcgg gctcgagctg gccgacggct gggcctccgg cggcaaggtc
     2461 gacctcgagg ccgtccgcgt cgcgctggcc gaggggcggg tgcgcctgcc gttcgccaca
     2521 tgaggccgac ggcggtgatg gcgcgcgcac ggggcggcgc ggtcgcggtg atcgcggccg
     2581 cgctcctctc gctcgcgcct atcgtcggcc gtgccgccgc gctcggcgcc gtcgacctcg
     2641 acgacgctac ggtcctggct gctcccccgc cgcgcgtcgc gggcagcgag gccgactata
     2701 cccgcgtcct ggtcgacgac gaggcctcgc tgtcggatcg cctgctggtg cacgtcgagg
     2761 ggccgaaggg cctgcccctg accgcgttca cggccgaacc tgatcggacg ctgttctcgc
     2821 ggctgcccga gagcggtagg cgaggcgtct ggaacgccct acgcgaggag ccgctcgcct
     2881 cgtcgactct gtggctatgc ttcaggctcg ataatcgctc gccgtgcgcg gcgtgggccc
     2941 tcgtcgtcga aggcgccggc ttcaagcctc tggcgatggt catcgtcggg ggcgacggcg
     3001 gtatccagca ttacggcgtc gagtccctcg acgccctgcg catggggacg agtctggccg
     3061 acctgtcggc gtacgcgctg aacgtaccgc cggaatcgga gcggaccgtc tacctgcgcc
     3121 tgtccgaggg ctcgctcttc ggccccggct tcggcgtatg gtcgcacggg ctcttcctgg
     3181 agcggtccga ggccaggctc ggcctgatga gcggcatagc cggcttcgcc gtagccgcct
     3241 gcgtgttcta cctgctcttg tcgtcgcgcg gctggcccgt tccatattcc ggtggcgtcc
     3301 gtcgcgccga ccaggcgctc tcctacgcca tgatgctgcg ttactccttc gtgctgcata
     3361 cgcaggcgga tctggtcgat gc
//
LOCUS       DAOOLN010000053         3381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_34025_length_3381_cov_3.155743, whole genome shotgun sequence.
ACCESSION   DAOOLN010000053 DAOOLN010000000
VERSION     DAOOLN010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34025_length_3381_cov_3.155743"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..624
                     /locus_tag="PLW80_01130"
     CDS             115..624
                     /locus_tag="PLW80_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPB65133.1"
                     /translation="MKESVFNQYLTFTLHDEHYAVSVGKVREVLEYTRITKLPRTADY
                     MKGIINLRGKGVPVIDLRLKFGMDETPVAKDTAIIVLDVEGDDGEVIVGALADSVHEV
                     VEIAGDRIEPAPRFGTKLAAEFIDGIGKRDDAFIVILNIDRIFNSDEVAMLRAQDAAA
                     VAETADTAK"
     gene            696..1916
                     /locus_tag="PLW80_01135"
     CDS             696..1916
                     /locus_tag="PLW80_01135"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPB65134.1"
                     /translation="MNPIDIYVNKSVPFKFRGQEMGFELSHALFSSFDIDAGSRLLLK
                     LVAKNVEDGLVGSILDVGSGVGVLGVACARGYPGASLRLRDRDALACAFSERNARRNK
                     VRGATVDRALFLDGLGSERFDLALCNVPAKAGPPVLDRFLRDLPGVLTDSGRGAVVVV
                     QTIAEAAAESIRGSGAEIVATERGPGHTALLFARGGAAAYADDGSSYAVERSERRGLR
                     AGKSVYAIKGYWGLPEFDTSSFATELAVELFEHASAGLLIRRAAVVNPGVGALASYVG
                     VRAKGATIDLCGRDSLALVASARNLALATRSGSEPGAAFAFQADLPDSAYDLLVEFPD
                     LTPRVDTTGDSWETAARVLKRGGAFVASMPSTAMDRFERRRPKGFTRLAEKKKKGFTC
                     AAWRFDGLEVSAEA"
     gene            2007..>3381
                     /locus_tag="PLW80_01140"
     CDS             2007..>3381
                     /locus_tag="PLW80_01140"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013944825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HPB65135.1"
                     /translation="MNLDCIRHPRLREWIDENVAMCMPDDVVVCDGGHEEADRLAERM
                     LRSGSFKRLEKRPNSFAVRSDPGDVARVENNTFICSRFREEAGPTNNWRDPQEMKGEL
                     RQRFFGCMRGRTMYVIPFSMGPIGGPLSQIGVELTDSVYVVLNMRIMTRMGEAVLKEL
                     DRDKPFVKCLHSVGYPLTLGRHELPWPCDSNNKYIVHFPEERSIWSYGSGYGGNALLG
                     KKCYALRIASVMARDQGWLAEHMLILGITNPDGKKKYIAAAFPSACGKTNLAMLVPNL
                     PGWKVETVGDDIAWLRWGDDGRLYAINPEYGFFGVAPGTSLKSNPNAMRSTEKNCIFT
                     NVATTPDGDVWWEGIGGEPPAGLVDWLGEPYDPSKGKPAAHPNSRFTAPASQCPVIDQ
                     AWEDPRGVPISAILFGGRRAGTVPLVHEAMDWEHGVFMGSIVSSETTAAAAGAVGQLR
                     RDPFAMLP"
BASE COUNT          582 a         1124 c         1129 g          546 t
ORIGIN      
        1 cctttcgtgg atacgcggag aggagcttag gcgcaacgca gcgatagccc tgggagccgg
       61 cgcatttgac gcggacgcgg acaggcgcta gagttagggc aggagtaccg agcgatgaaa
      121 gagagtgtct tcaaccagta cctgaccttc acgttacacg acgagcacta cgccgtatcg
      181 gtcggcaagg tgcgggaggt cctcgaatat acccgtatca ccaaactgcc gcgcaccgcc
      241 gactacatga agggcatcat caacctgcgc ggcaaaggcg tgccggtgat cgacctgcgg
      301 ctcaagttcg gcatggacga gacgcccgtc gccaaggata cggccatcat cgtgctcgac
      361 gtcgagggcg atgacggcga ggtgatagtc ggcgccctgg cggactcggt ccacgaggtg
      421 gtcgagatag ccggggaccg tatcgagccg gctccccgat tcggtaccaa gctcgcggcg
      481 gagttcatag acggcatagg caagcgcgac gacgcgttca tcgtcatcct gaacatcgac
      541 cgcatcttca actcggacga ggtggccatg ctgcgagccc aggacgcggc tgcggtcgcc
      601 gagaccgccg atacggcgaa gtagcctata cgcccgtgca gggcgggccc gcccggcgct
      661 tgctacgaag gccgggccgt cgtactatgg ccgagatgaa ccctatcgat atatacgtca
      721 acaagagcgt cccgttcaaa ttcaggggcc aggagatggg cttcgagctg tcccacgccc
      781 tgttcagctc cttcgacata gacgcgggct ccaggctgct cctcaagctc gtggccaaga
      841 acgtcgagga cggcctcgtc ggctcgatac tcgacgtcgg ctccggcgtc ggcgtgctgg
      901 gagtcgcctg cgccaggggc tacccgggcg ccagtctgcg cctgcgcgac cgcgacgccc
      961 tcgcctgcgc cttcagcgag cgcaacgcca ggcgcaacaa ggttcgcggc gccacggtcg
     1021 accgggccct gttcctcgac gggctcggtt ccgagcgctt cgacctggcg ctgtgcaacg
     1081 tgccggccaa ggccggcccg ccggtcctgg atcgcttcct gcgcgacctt cccggcgtcc
     1141 tgaccgactc ggggcgcggg gctgtcgtcg tcgtgcagac gatagccgag gccgcggccg
     1201 agtccatccg tggttcaggg gccgagatcg tggcgaccga gcgcgggccc gggcatacgg
     1261 ccctgctgtt cgccagggga ggcgccgccg cgtacgcaga cgacgggtcg tcgtacgccg
     1321 tcgagcgttc ggaacgacgg ggcctgcggg ccggcaagtc cgtctacgcg atcaagggct
     1381 actggggcct ccccgagttc gacacctcgt ccttcgccac ggagctcgcc gtcgagctgt
     1441 tcgagcacgc ctcggccggc cttctcatca ggcgggcggc cgtcgtcaat ccgggagtcg
     1501 gggcgttggc cagttacgtc ggggtccgcg ccaaaggcgc gacgatagac ctgtgcggcc
     1561 gcgactcgct ggcgctcgtc gccagcgcca ggaacctggc gctggccacc cggtccggtt
     1621 ccgagccggg cgccgccttc gcgttccagg ccgacctgcc cgattcggcc tacgacctgc
     1681 tcgtcgagtt ccccgacctc acgccgcgcg tcgacacgac cggcgactcg tgggagacgg
     1741 ccgctagggt gctcaagcgc ggcggggcct tcgtcgcctc gatgccgtcg acggcgatgg
     1801 accgtttcga gcggcggagg cccaagggct tcacgcgcct ggccgagaag aagaagaagg
     1861 gcttcacctg cgccgcgtgg cgcttcgacg gcctggaggt ctcggcagaa gcctaaggtc
     1921 caggcgccgt tccggccgcg atcgttttat cttgtgcaat acgcgtttcg ggtttaaaac
     1981 tgtccgcgaa gaaggagacc ctcggtatga acctcgattg catcaggcat ccacgcctcc
     2041 gcgagtggat agacgagaat gtggccatgt gcatgccgga cgacgtggtc gtctgcgacg
     2101 gcgggcatga ggaggccgat aggctggccg agcgcatgct ccgctccgga agcttcaagc
     2161 ggctggagaa gcgtcccaac tcgttcgcgg tccgtagcga cccgggagac gtcgcccgcg
     2221 tcgagaacaa taccttcatc tgctcgcgtt tccgcgaaga ggccggccca acgaacaact
     2281 ggcgagatcc ccaggagatg aagggcgagc tcaggcagcg cttcttcggg tgcatgcgcg
     2341 gtcgcaccat gtatgtcatt ccgttttcga tggggcccat aggcggcccc ctgtcccaga
     2401 taggcgtcga actgaccgac agcgtctacg tcgtgctgaa catgcgcatc atgacgcgca
     2461 tgggcgaggc cgtgctcaag gagctcgacc gcgacaagcc cttcgtcaag tgcctgcatt
     2521 cggtaggcta cccgctgacg ctcggccggc acgagctacc ctggccctgc gactcgaaca
     2581 ataagtatat cgtccacttc cccgaggaac gctcgatctg gtcgtacggc tcgggctacg
     2641 gcggcaacgc gctgctcggc aagaaatgct acgcgctacg catcgccagt gtcatggcgc
     2701 gggatcaggg ctggctcgcc gaacatatgc tcatcctcgg catcacgaat cccgatggga
     2761 agaaaaagta catcgcggcg gccttccctt cggcctgcgg caagaccaac ctggccatgc
     2821 tcgtgccgaa cctgccgggc tggaaggtgg agacggtcgg agacgacatc gcctggctgc
     2881 gctggggcga cgacgggcgg ctctacgcca tcaacccgga gtacggcttc ttcggcgtcg
     2941 caccgggcac cagcctcaag tccaatccca acgcgatgcg ctccacggag aagaattgca
     3001 tcttcacgaa cgtggccacg acgccggacg gcgatgtctg gtgggagggc ataggcgggg
     3061 agcctccggc cggcctcgtc gactggctcg gcgagcccta cgacccgtcc aagggcaagc
     3121 cggccgccca cccgaacagc cgcttcaccg cccccgcgtc ccagtgccct gtgatcgacc
     3181 aggcctggga ggatcccagg ggagtgccga tcagcgccat cctgttcggc gggcgcaggg
     3241 cgggtaccgt accgctcgtc catgaggcga tggactggga gcatggcgtg ttcatgggtt
     3301 ccatagtctc gagcgagacc accgccgccg ccgccggcgc cgtcggccag ctcaggcgcg
     3361 accccttcgc gatgctgccg t
//
LOCUS       DAOOLN010000054         3344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_34526_length_3344_cov_3.468531, whole genome shotgun sequence.
ACCESSION   DAOOLN010000054 DAOOLN010000000
VERSION     DAOOLN010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34526_length_3344_cov_3.468531"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1402
                     /locus_tag="PLW80_01145"
     CDS             <1..1402
                     /locus_tag="PLW80_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPB65136.1"
                     /translation="PAIELSAAETLFSPNGDGRKDVLSIRQTSTPGDTWEGAILDSAG
                     RVMRAWTWKDAAVDFDWDGADSEGNKVADGAYRYVVRSEDPAGNKTEKAIQRMVVDTR
                     QTQAFVTASAAGFSPNGDGMFDEMSFGLVVKLTDGIDSWKLAMVGDDGVTRRSFTGKG
                     ATIPAKLAWDGKSDDGQLRQGAYTAVFTVDYLKGDRAQASSATFSLDTEGPKVAMTTS
                     PRYFSPDNDGVDDELRISLAVSDASVIDSWRFDIVEVAVSESAGAKRAERVFFSWSGR
                     GKPAERLAWDGRSQKGELVEAATDYPYRLTIVDELGNQTVSEGLISVDVLVIRDGDRL
                     KIKVPSIVFRPDFADFKDLPQETLDRNAEVLKRIAQILNRFKDYKIRVEGHANSIAKM
                     TGAGQPAIDREETRELLPLSENRAALVMQKLIEYGVDPKRLSVRGLGSSEPVVPFTDA
                     ENRWKNRRVEFILIKE"
     gene            1406..1642
                     /locus_tag="PLW80_01150"
     CDS             1406..1642
                     /locus_tag="PLW80_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65137.1"
                     /translation="MPYEGVLAIVSIFIVAPCIVFGFILLAKRGRNRVDLARCAVREK
                     ELELERERLRLEALQLENRKLDLMIEHRIDSLKP"
     gene            1738..2634
                     /locus_tag="PLW80_01155"
     CDS             1738..2634
                     /locus_tag="PLW80_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HPB65138.1"
                     /translation="MPIPEYPEFAPVTVDMRDDLYPALNLLADGVSEFTFSGLYLFRD
                     TYKYTASHISGSTYVISGEKRGERFFFTPCCIPPNATLERLFEDHCYLKNMSESQCKA
                     ERIRLEAAGYEVHEDRDNFDYLYDRKDLAELSGKAYHKKRNLVNAFLNSYSYEQKELD
                     ASNVADAIRVLDAWRVEKGIDGDYAAAREALELYERLGMRGAVYYVDGKPAGYALGEP
                     IAKARMFAVHFEKATGDYKGIYQFINQAFAQALPPYFRQVNREQDLGDEGLRQAKMTY
                     RPSGFVRKYRVMPTKPSFLPEA"
     gene            2638..>3344
                     /locus_tag="PLW80_01160"
     CDS             2638..>3344
                     /locus_tag="PLW80_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65139.1"
                     /translation="MGGRMSFDELTTTGLSRAFSARRGLPEAAASTLGVRDSDGLALE
                     LAGRTCCLTMLAGSGSRWVASLEAARAAGRDMGADPRAPRGLYPVRNAMPFGPDPAPI
                     ASYALAAVRDLGHHVIVVRGWEREIEERILGPFGFAPGSYRFATQGAPGGKPRGHGDA
                     AFQTLGLWSGYDYVTVNFGGDASSPLTALASLAAMAALERELGDRAPGLLLPAALVDS
                     PAYPIGLGPGGLPASFG"
BASE COUNT          571 a         1164 c         1086 g          523 t
ORIGIN      
        1 gcccgccata gagctgtccg ccgccgagac cctgttcagc ccgaatggcg acggccgcaa
       61 ggatgtcctc tccatacgcc agacgtcgac gcccggtgac acctgggaag gggccatcct
      121 cgactcggcc ggccgcgtca tgcgcgcctg gacctggaag gacgccgccg tcgacttcga
      181 ctgggacggc gccgattccg aggggaataa ggtcgccgac ggcgcctacc gctacgtcgt
      241 gcgatccgag gacccggccg gcaacaagac cgagaaagcc atccagagga tggtcgtcga
      301 taccaggcag actcaggcct tcgtcaccgc cagcgcagcc ggcttcagcc cgaacggcga
      361 cggcatgttc gacgagatgt ccttcggcct ggtcgtgaag ttgaccgacg gcatcgattc
      421 ctggaagctg gccatggtcg gcgatgacgg cgtcacgcgc cgctcgttca cgggcaaggg
      481 cgcgaccatt cccgccaagc tggcctggga cggcaagtcc gacgacggtc agctcaggca
      541 gggcgcctat accgccgtct tcacggtcga ttacctcaag ggcgaccgcg cgcaggcctc
      601 cagcgcgaca ttctccctcg ataccgaggg gccgaaggtc gccatgacga cctcgccccg
      661 ttatttcagc cccgataacg atggcgtcga cgacgagctc aggatatcgc tcgccgtctc
      721 cgacgccagc gtgatcgact cctggcgctt cgacatcgtc gaggtggccg tgagcgagag
      781 cgccggcgcc aagcgggcgg agcgcgtctt cttctcgtgg agcggccgcg gcaagccggc
      841 cgagcgcctg gcctgggacg gccgctcgca gaagggcgag ctggtcgagg ccgcgaccga
      901 ttacccgtac cgcctgacca tcgtcgacga gctgggcaat cagacggtct ccgaggggct
      961 catctccgtg gacgtcctgg tcatccgcga cggcgaccgg ctcaagatca aggtcccgtc
     1021 gatcgtgttc aggccggact tcgccgactt caaggacctg ccgcaggaga ccctcgaccg
     1081 caacgccgag gtgctcaagc gcatcgccca gatcctcaac cgcttcaagg actacaagat
     1141 ccgggtggag ggccacgcca actcgatcgc caagatgacc ggggcaggcc agccggcgat
     1201 cgaccgggag gagaccaggg agctcctgcc gctgtcggag aatcgggccg cgctcgtgat
     1261 gcagaagctc atcgagtacg gcgtcgaccc gaaacgcctg tcggtacggg ggctgggctc
     1321 ctccgagccg gtcgtgccgt tcaccgacgc cgagaaccgg tggaagaacc gccgcgtcga
     1381 attcatcctg atcaaggaat agagcatgcc gtacgaggga gtcctggcca tcgtgtccat
     1441 cttcatagtg gcgccgtgca tcgtgttcgg cttcatcctg ctcgccaagc gcggccgcaa
     1501 tcgggtggac ctggcccgtt gcgctgtccg cgagaaggag ctcgagctgg agcgcgagcg
     1561 cctgcgcctc gaggccctgc agctcgagaa ccgcaagctc gacctgatga tcgagcatcg
     1621 gatcgactcc ctcaaaccct agccggggcg atcgcctcat caaggcccgg gacgcgcatt
     1681 catcgcggcc cgggcctttc cttgattcta tcgggaattt tgcatatagt cgtaggcatg
     1741 ccaataccag aataccctga attcgccccg gtgaccgtcg acatgcgcga cgacctctac
     1801 ccggccctca acctgctagc ggacggcgtg tcggagttca ccttctccgg cctctacctg
     1861 ttccgggaca cctacaagta caccgcgtcg catatcagcg gctccaccta cgtaatctcc
     1921 ggcgagaagc gcggggagcg cttcttcttc acaccgtgct gcatcccgcc gaacgccacg
     1981 ctcgagcgcc tgttcgagga tcactgctac ctcaagaaca tgtccgagag ccagtgcaag
     2041 gccgagcgca tccgcctcga ggccgccggc tacgaggtcc acgaggaccg cgacaacttc
     2101 gactacctgt acgaccgtaa ggatctcgcc gagctgtcgg gcaaggcgta ccataagaag
     2161 cgcaacctgg tgaacgcctt cctgaactcg tactcgtacg agcagaagga gctcgacgcc
     2221 tcgaacgtcg ccgacgctat ccgcgtcctg gacgcgtggc gggtcgagaa aggcatagac
     2281 ggcgactacg cggccgccag ggaggccctc gagctgtacg aacgcctggg catgcgcggc
     2341 gcggtttact atgtcgacgg caagccggcc ggctacgcgc tcggcgagcc tatcgccaag
     2401 gcccgcatgt tcgccgtgca cttcgagaag gcgaccggcg actataaggg catctaccag
     2461 ttcatcaacc aggccttcgc ccaggccctg ccgccctact tcaggcaggt caaccgcgaa
     2521 caggacctcg gcgacgaggg gctcaggcag gccaagatga cctaccgccc cagcggcttc
     2581 gtgcgcaagt accgcgtcat gccgacgaag ccgtccttcc tgcccgaggc ttaggcgatg
     2641 ggcgggcgga tgagcttcga cgagttgacg acgaccggcc tgtcgcgggc cttctcggcc
     2701 cgtcgtggcc tgcctgaggc ggccgcgtcg acgctaggag tccgggactc ggacgggctg
     2761 gcgctcgagc tcgctgggcg gacctgttgc ctgaccatgc tcgccggctc gggctcgcgc
     2821 tgggtagcct cgctggaggc ggccagggcg gccggccggg atatgggcgc cgatcccaga
     2881 gcgccgcgcg gcctctatcc cgtccgtaac gccatgccct tcgggccgga cccggcgccg
     2941 atcgcttcgt acgcgctcgc ggccgtacgc gacctcggcc accacgtcat cgtcgtgcgg
     3001 ggctgggagc gcgagatcga ggagcgcatc ctcgggccgt tcggcttcgc gccaggctcg
     3061 tatcgcttcg cgacccaggg cgcccccggc ggtaagccgc gcggccatgg cgacgccgcg
     3121 ttccagacgc tcggcctatg gtccggctac gactacgtga cggtgaactt cggcggcgac
     3181 gcctcgagcc cgttgaccgc cttggcctcg ctcgccgcga tggccgcgct ggagcgcgaa
     3241 ctcggcgacc gggccccggg gctcctcctg ccggcggccc tcgtcgactc gccggcctac
     3301 ccgataggcc tagggcccgg cggcctaccg gcgagcttcg ggca
//
LOCUS       DAOOLN010000055         3339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_34590_length_3339_cov_2.851096, whole genome shotgun sequence.
ACCESSION   DAOOLN010000055 DAOOLN010000000
VERSION     DAOOLN010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34590_length_3339_cov_2.851096"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PLW80_01165"
     CDS             <1..311
                     /locus_tag="PLW80_01165"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPB65140.1"
                     /translation="ELPLLAYVTGAAVFVTLFFEEALWHNHVCPYGTILSLTSRKAVK
                     SEKIDEAACVSCGKCRTVCPVDCIDIVADTKKMRIRTPDCLTCHACEAVCPIGAISYR
                     "
     gene            392..868
                     /locus_tag="PLW80_01170"
     CDS             392..868
                     /locus_tag="PLW80_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPB65141.1"
                     /translation="MQDCDYSGAETGLVPLPLTPETVRELWSRTYNTAGKPDWSHTFP
                     YYHPDIVFQDSIQRVEGIEAFKALCGRLTDRCEELRMDIHSVARSGRSIHLQWTMTMM
                     FKKFPSTPVYGATVLTLHEDGRIIGQRDYYDLWGDIFNGVPWFKGPYRRFMARKFG"
     gene            complement(900..2525)
                     /locus_tag="PLW80_01175"
     CDS             complement(900..2525)
                     /locus_tag="PLW80_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB65142.1"
                     /translation="MEYDAVVVGAGMAGLTAAAYLCKAGKSVALLEKRGKTGGLVDSF
                     ERNGFTFDGGIRAVENSGIVFPMLRQLGVELEFLQSPVSIGFGDDVLRLSPEAGLDEY
                     RALLRRRFPGIVSEVDAIVDEVARVMDYMAVLYGIDNPLFLDLKDDPGYVLKVILPWM
                     LKYMTTMPKVARLRMPVDERLAALTGSRELADMIGQHFFKKTPAYFALSYFSLYLDYR
                     YPRGGTGRLPEALESYIRDRGGSVMTGVEVAGLDAARRVVTDASGREYGYRRLVWAAD
                     SRLLYRLALASPLADRGVARRVEAKAAELASARGGDSIFTLYVAADLPPVFFSSVSSA
                     HFFYTPRGEGLSSLGPWPLEAKNEVDVAQDDVAAGEDPDKEALLAWLGDYLERTTYEI
                     SIPVLRDPTLAPEGKAGLIVSTLMDYDLAKRAEERRWYDEYKEFCSARIVDILDRGAY
                     PCLRASVIERTASTPVTIRRETGNTDGAITGWAFTNPMMPAVNDLPKVTRSIRTPIPG
                     VYQAGQWTFSPSGLPISILTGKLAADAVVKDLR"
     gene            2692..>3339
                     /locus_tag="PLW80_01180"
     CDS             2692..>3339
                     /locus_tag="PLW80_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPB65143.1"
                     /translation="MPNRTNSLSLPPVIAVDEGTCVNCHMCIAVCPVKYCIDGSGDKV
                     TINPDLCIGCGSCVKVCTQKARVIIDDAARFVEALGRGEKLVAVVAPAIAASYPGEHR
                     RFLGWLKGLGVSACFDVSFGAELTVRSYLRHIEATRPELVIAQPCPAIVNYIEIYRPE
                     LLGYLAPADSPMLHTIKMIREYYPAYRDHKILVVSPCAAKKREFEETGAGDYNVTL"
BASE COUNT          605 a         1085 c         1050 g          599 t
ORIGIN      
        1 aggagctccc gctcctggcc tacgtgaccg gggcggccgt tttcgtgacg ctgttcttcg
       61 aggaggccct gtggcataac cacgtgtgcc cgtacggcac aatcctcagc ctgacgtcac
      121 gcaaagccgt caagagcgag aagatcgacg aggcggcctg cgtctcctgc ggcaagtgcc
      181 gcacggtatg ccccgtcgac tgcatcgata tcgtcgcgga cacgaagaag atgcgcatac
      241 gcacgcccga ctgcctgacc tgccatgcct gcgaggccgt atgtcctata ggggctattt
      301 cgtaccggta atgaattgtc cgacgccggc tacatgaaca gcgtggccgg cgagcttgcc
      361 gcctagcccc tctcgtgtta ccgtggaaac catgcaagac tgtgattatt cgggtgccga
      421 aaccggtctc gtcccgttgc ctctgacgcc cgagacggtc agggaactat ggtcgaggac
      481 ctacaacaca gcgggcaagc cggattggtc gcataccttc ccgtactatc atccggatat
      541 cgtcttccag gactcgatcc agcgggtcga gggcatcgag gcgttcaagg cgctctgcgg
      601 gaggctcacg gaccgctgcg aggagctgcg catggatatc cactcggtcg cgcgttcggg
      661 ccgctccatc cacctgcagt ggacgatgac gatgatgttc aagaaattcc cgagcacgcc
      721 cgtctacggc gccacagtgc tgaccctcca cgaggacggc cgcatcatcg gccagcgcga
      781 ctattacgac ctttggggcg acatcttcaa cggcgtaccg tggttcaagg ggccgtaccg
      841 ccggtttatg gccaggaaat tcggctaggc ccgcggcggg cccgcggctg ggccgtggcc
      901 tagcgcaggt ccttgacgac agcgtcggcc gcgagcttgc cggtcaggat cgatatcggc
      961 agccccgacg ggctgaaggt ccattggccg gcctggtaca cgccaggtat cggcgtgcgt
     1021 atggatcgcg tcaccttcgg caggtcgttc acggccggca tcatcgggtt cgtgaaggcc
     1081 cagccggtga tcgcgccgtc ggtgttgccg gtctcgcgcc gtatggtcac cggggtcgag
     1141 gcggtccgct cgatcacgga ggctcgcaag cagggatagg ccccgcggtc gagtatgtcg
     1201 acgatgcgag ccgaacagaa ttccttgtac tcgtcgtacc agcgccgttc ctccgcgcgc
     1261 ttggccaggt cgtagtccat cagcgtgctg acgatcaggc ccgccttgcc ctcgggcgcc
     1321 agcgtggggt ccctgaggac cggtatcgat atctcgtagg tggtacgctc gagatagtct
     1381 ccgagccagg ccagcagggc ttccttatcg ggatcctccc cggccgcgac gtcgtcttgg
     1441 gcgacgtcga cctcgttctt ggcttcgagg ggccaaggtc cgagcgacga caggccctcg
     1501 ccgcgcggcg tgtagaagaa gtgagcgctc gacacggacg agaagaacac gggcggcagg
     1561 tcggcggcga cgtacagcgt gaagatcgag tccccgccgc gcgccgacgc gagctcggcc
     1621 gccttggcct cgacgcgcct ggcgacgccc cggtcggcta gcggagaggc cagcgccagc
     1681 cggtacagga gccgcgagtc ggctgcccag acgagcctgc ggtagccgta ctcgcgcccg
     1741 gacgcgtcgg tgacgacccg acgggccgcg tcgaggccgg cgacctcgac gccggtcatc
     1801 accgagccgc ctcggtcgcg tatatacgac tcgagggcct cgggtaaccg gccggtgccg
     1861 cccctggggt agcgatagtc gaggtacagg ctgaagtacg acagggcgaa gtaggccggc
     1921 gtcttcttga agaagtgctg gccaatcata tcggccaact cgcggctgcc ggtcagggcc
     1981 gccaaccgct cgtccaccgg catgcgcagc ctggccacct tgggcatggt cgtcatatac
     2041 ttgagcatcc aaggcaggat gaccttgagt acgtagcccg gatcgtcctt cagatcgagg
     2101 aacaaggggt tgtcgatgcc gtagaggacg gccatgtaat ccatcaccct ggccacctcg
     2161 tcgactatcg cgtcgacctc ggagacaatc ccggggaaac ggcggcgcag caaggcccgg
     2221 tattcgtcga gaccggcctc gggcgatagc ctgagtacgt cgtcgccgaa gcctatcgac
     2281 acggggctct gcaggaactc cagctcgaca ccgagctgcc tgagcatcgg gaacacgatg
     2341 cccgagttct cgacggcgcg tatgccgccg tcgaaggtga acccgttccg ttcgaacgaa
     2401 tcgacgaggc cgccggtctt gcctcgcttc tcgagcaagg ctaccgattt gcccgcttta
     2461 cagagatagg cggcggcggt cagccccgcc atgcctgcgc cgaccacgac ggcatcgtac
     2521 tccatggttc ttcctgacgt ttcagtatag aggaaagcct atcaaactat tacctcgggt
     2581 cggcggcttg tcaatctcga tttgatcgcc cggccatgtt actatacgtg atcgcttgca
     2641 catgaactcg ctcgtgtata gaattcgacc ccaatacgga gactagccac catgccgaac
     2701 cgaacgaact ccttgtcgct accgccggtc atagcggttg acgagggtac ctgcgtcaat
     2761 tgccatatgt gtatcgccgt ctgcccggtc aagtactgta tcgacggctc gggcgacaag
     2821 gtaacgataa accccgatct gtgcatcggc tgcggtagct gcgtcaaggt ctgtacgcag
     2881 aaggcgcgcg tcatcattga cgatgctgcc aggttcgtcg aggccctcgg ccgtggcgag
     2941 aagctcgtcg cggtagtcgc gccggccata gccgcgagct acccgggcga acaccgccgc
     3001 ttcctcggct ggctcaaggg cctcggcgta tcggcctgct tcgacgtcag cttcggcgcc
     3061 gagctgaccg tccgctccta cctgcgccat atcgaggcga cgcggcccga gctcgtcatc
     3121 gcccagccgt gcccggccat cgtcaactac atcgagatct acagacccga gctactcggc
     3181 taccttgccc cggccgatag cccgatgctc cataccatca agatgatacg ggagtactat
     3241 ccggcctatc gcgaccacaa gatactcgtc gtctcgccct gcgcggccaa gaagcgcgaa
     3301 ttcgaggaga ccggcgccgg cgactacaac gtgacgctc
//
LOCUS       DAOOLN010000056         3333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_34665_length_3333_cov_4.244356, whole genome shotgun sequence.
ACCESSION   DAOOLN010000056 DAOOLN010000000
VERSION     DAOOLN010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34665_length_3333_cov_4.244356"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..>3333
                     /locus_tag="PLW80_01185"
     CDS             38..>3333
                     /locus_tag="PLW80_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65144.1"
                     /translation="MKTQAVFPVFRSWYDAEPLGSVTIRNDEDGPIDNLRVSFFAPEY
                     MGGPRLCSVVRRVEKGESVTVPLYAVFDERVLTLTENGSTSAEIAVEYSYVGTRRTAK
                     EGLSLSLHHRNAMSWEDDRRAAAFVSPTDPAVLWFSRYSASVVRDRLRTELPPNLQYA
                     LGLFEALKLYGLNYVIDPNSAYVDMSESAAVVDYLQYPSQTLMYRGGDCDDLSILFCS
                     LLESQGIATAFITIPGHIYMAFDLGLSEAEARERFFDPGLLLFRDGRAWAPVEITMVK
                     DGFVKAWRVGAKQWIDNDRAGTAAFYAMRDAWTEYKPSGLQDAATRFSLPDEARAMAA
                     FDSGVDRFVAREMEPVVAEYERKLARRREPEVLNEYGTAMARVGMLDQAWERFAEASK
                     AGYAWAWNNLASIAFVRKDYELAYSYYEWTTTLLPGDPVAVLGMARCAYELDRYAEAA
                     ILYEALTVDAPALAVKYGYLASAYGGTGRAWSMADRLAGSVWSRPDMSFDLPSSAVAP
                     AVARTAPVAPTPAPAARIAPPPADDTPAETEKVAVPTPTASPEPAVEPEPAAKAEPAV
                     EPEPATEPEPVVEPEPAAEPESAQAVKQQGSASIQAVAAAKALAKEAKPAEPVAKTSA
                     TPSSIATVAAPAPSAAIAPPAVVDKPPEDREIAAKAAEAAIVAPPAVIERPPEDREIA
                     AKAAIAPTPSDTTSAQDPVATPTPKPVVVEPPAEIARTQEPVAVAPKPVVVAPPAETA
                     RAPVAEAAAEVMRVQEPIAVAPKPVVVAPPAETARTQEPVNAAPEPVAVEPKPVVVEP
                     PAPKAVEAPTPADTAPVVERRPAATTPAAAAAEDASGLAQEGGPIPDPTPSQRPVVDL
                     DHTSVSEAGLAERPAAPAEADGKPIRSAYAPAARFAPTVEAHAAPAFGKRLPDGADAS
                     GYERLPVSAEPPRARTIDLRAPEIRVGSGTWKREESRAAMTDPKAYYAKLVIPTPGLS
                     GTTAYEFKARATGSGWVGFGLHVHGRGAWRLTGYGGGDSLLVWVTSDPKAYGDAAPRL
                     QVYRSRGEVAMDLLAEARLVGSAFEDAAYRVEYDPAAGSVRAYVNGALALSASGIANP
                     MGNAVKNYFA"
BASE COUNT          539 a         1227 c         1124 g          443 t
ORIGIN      
        1 gacgggttct tctgcggcgc ggctcagctt ccgctcggtg aagacccagg ccgtcttccc
       61 ggtcttccgc tcctggtacg acgcggagcc gctcggctcg gtgacgatac gcaacgatga
      121 ggacggcccg atagacaacc tgcgcgtctc gttcttcgcg cccgagtaca tgggcggccc
      181 gaggctgtgc tcggtcgtcc gacgcgtcga gaagggcgag agcgtcaccg tgccgctcta
      241 cgccgtcttc gacgagcgcg tgctgacgct gacggagaac ggctcgacga gcgccgagat
      301 agcggtcgag tactcgtacg tcggcacgcg gcgaacggcc aaggaaggcc tgagcctgtc
      361 gctccaccac cgcaacgcga tgtcctggga ggacgaccgg cgcgccgccg ccttcgtctc
      421 gccgaccgac ccggcggtgc tctggttctc ccgctactcg gccagcgtcg tgcgcgaccg
      481 actgcgcacc gagctgccgc cgaacctgca gtacgcgctc ggcctcttcg aggcgctcaa
      541 gctctacggg cttaactacg tcatcgaccc gaacagcgcc tacgtcgaca tgtccgagag
      601 cgccgccgtc gtcgactacc tgcagtatcc gagccagacc ctgatgtacc gcggaggcga
      661 ctgcgacgac ctgtccatcc tgttctgctc cctgctcgag tcgcagggca tagcgacggc
      721 cttcatcacg atcccaggcc atatctacat ggccttcgac ctgggcctct ccgaggccga
      781 ggccagggag cgtttcttcg accccggtct gcttctgttc agggacggcc gcgcctgggc
      841 gccggtcgag ataacgatgg tcaaggacgg cttcgtcaag gcgtggcgcg tcggcgccaa
      901 gcagtggata gacaacgatc gggccggtac cgcggccttc tatgcgatgc gcgacgcctg
      961 gaccgagtat aagccgagcg gccttcaaga cgccgcgacc cgcttctcgt tgcccgacga
     1021 ggcccgcgcg atggccgcct tcgactcggg tgtcgaccgc ttcgtggccc gcgagatgga
     1081 gccggtcgtc gccgagtacg agaggaagct ggccaggcga cgcgagcccg aggtactcaa
     1141 cgagtacggc acggcgatgg cccgcgtcgg catgctcgac caagcctggg agcgtttcgc
     1201 cgaggcctcc aaggccggct acgcctgggc ctggaacaac ctggccagca tcgccttcgt
     1261 acgcaaagac tacgagctgg cttattcgta ctacgaatgg acgacgaccc tgctgcccgg
     1321 cgaccccgtc gcggtgctcg gcatggcgcg ctgtgcctac gagctggacc gctacgccga
     1381 ggcggccata ctctacgagg ccctcaccgt cgatgccccg gcgctggctg tcaagtacgg
     1441 ctacctggcc tcggcctacg gcgggaccgg gcgcgcctgg tccatggccg accgcctcgc
     1501 cggctccgtc tggtcgaggc ccgacatgag cttcgacctg ccctcgagcg ccgtggcgcc
     1561 ggcggtggcc aggaccgcgc cggtagcccc gacgcccgcc ccggcagccc ggatcgcgcc
     1621 gccgccggcc gacgacacgc ctgccgagac cgagaaggtc gccgtgccta cgccaactgc
     1681 gagccccgaa cccgcggtcg agcccgagcc ggcagccaag gccgagccag cggtcgagcc
     1741 cgagccggct actgagcccg aaccagtcgt ggagccggaa cccgcggccg agcccgagtc
     1801 ggcacaagca gtcaagcaac agggctctgc ctcgatccaa gccgtcgcgg ccgccaaggc
     1861 gctcgcgaaa gaagccaaac cggccgagcc cgtggcaaag acgtccgcta ccccgtcgtc
     1921 catagcgacc gtagccgcgc cggccccgag cgccgcgata gcgcctccgg ccgtcgtgga
     1981 taagccgcca gaggacaggg agatcgccgc caaggccgcc gaggccgcaa tcgtcgcgcc
     2041 acccgcagtc atcgagcggc cacccgagga tagggagatc gccgcgaagg ccgcgatagc
     2101 gccgacgccg tctgatacga cgagcgccca ggatccggtc gccacgccaa cgccgaagcc
     2161 cgtcgtggtc gagccgccgg ccgagatcgc gcggacacag gaaccggtag ccgtcgcgcc
     2221 gaagcctgtc gtcgtagcgc cgccggcaga aacggcgcgg gcgccggtcg ccgaagcggc
     2281 ggcggaggtg atgcgcgtgc aagagccgat agccgtggcg ccgaagcccg tcgtcgtagc
     2341 gccaccggcc gaaacggcac ggacgcagga accggttaac gcagcaccag agccggtcgc
     2401 tgtcgaaccg aagcccgtcg tggtagagcc gccggcgccg aaggcggtcg aagctccgac
     2461 gccggccgat acggctcccg tcgtcgagag gcggccggcc gcgacgacac cggccgcggc
     2521 agcggccgag gacgcgtccg ggctcgctca ggaaggcggc ccgatacccg atcctacgcc
     2581 atcgcaacgt ccagtagtcg accttgacca tacaagcgtc tcggaagccg ggttggccga
     2641 gcgaccggcg gctccggccg aggccgacgg aaagccgatc cggtcggcct acgcgccggc
     2701 cgcccgcttc gcgccgaccg tcgaggcgca cgccgcgccg gccttcggga agcggctgcc
     2761 cgacggcgcg gacgcgtccg gctacgagcg cctgcccgtc tccgccgagc cgccccgcgc
     2821 tcggaccata gacctgcgag cgccggagat tcgcgtcggc tcaggcacct ggaagcgcga
     2881 ggagagccgc gccgcgatga ccgaccccaa ggcctactac gccaagctgg tcatcccgac
     2941 gcccggactg tccggtacga ccgcctatga gttcaaggcg cgcgccaccg gttccggctg
     3001 ggtcggcttc ggcctccacg tccacgggcg cggagcctgg aggctgaccg gttacggcgg
     3061 cggcgactcc ctgctcgtct gggtgacgag cgaccccaag gcctacggcg acgccgcgcc
     3121 ccgcctccag gtctaccggt ctcgcggcga ggtggccatg gacctgctcg ccgaggcgag
     3181 gctggtcggc tccgccttcg aggacgccgc ctaccgcgtc gaatacgacc cggcggccgg
     3241 ctccgtacgc gcctacgtga atggagccct cgccctctcg gcctccggca tcgccaatcc
     3301 gatgggcaat gcggtgaaga actacttcgc cat
//
LOCUS       DAOOLN010000057         3333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_34667_length_3333_cov_3.785540, whole genome shotgun sequence.
ACCESSION   DAOOLN010000057 DAOOLN010000000
VERSION     DAOOLN010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34667_length_3333_cov_3.785540"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PLW80_01190"
     CDS             complement(<1..819)
                     /locus_tag="PLW80_01190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB65145.1"
                     /translation="MPVERRKPFSAASALRRAAAYAASFPKPIWLLVAATLVESTGRF
                     MVVPYLSLYMKGEGVSLGALGFVLGAAPIASVAFGAWGGHLADRWGRKPVQILGVSLS
                     ASALFGFAFAGSNVYVLALLNFLNGMTRTFYRPATNAAIADFAPPERRSEAYALHRIA
                     INAAFGWGPLIGVGLFLVAPRTGFIVSGVLNLLVGVFIALAIPESSSPSESSSPSESS
                     SPLESASPLESAQVGERLDRLGAATRPAWRAIAADGVFWLWTFGMMLVWGAYDLI"
     gene            923..3322
                     /locus_tag="PLW80_01195"
     CDS             923..3322
                     /locus_tag="PLW80_01195"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HPB65146.1"
                     /translation="MNRKHSRAPWRFLTVAALFVVATGAFAQSGGSADPHTVWDYADN
                     PAAWSLFPDAIAFGVRAEAAVSDLASFAGGTLPATVDLAYGLPGLVYRYRDDGSSSTH
                     ALTSAFSFGRSFGLGLRFRWDGTTTSAWGVQDLGVMLRPFGYASVSASVDDVLDLSGD
                     GYEAALGLAVRPLAFNPRLGSALTLSADARLGPDGFAMAGAGARFVLADWLSLSGSYS
                     FDDASFGLGFAVSLSGAETSVGGRLNGMHQGGDVSLGQSVRLGRAAKSAERVFGSSVL
                     VIDEPGLFQPNPPRVDMDPGFGDEVALWFDLAVAAIDRAASDASIRALAMFEPPLFDT
                     DARAQEFGRALGRFRAAGKPVYVFAQSMGRLSYVYAAAGAELVALDPNGGLPIVDVSS
                     THLYFKDLLARLGVRTYSLQSHDTKTANNAMTEPSMTGPERAMIERYVGGLASQAWAS
                     LDEARGDRVVGGGRAAIGAGPYLDPRKAVEAGLADELLYRDEFEERLTELTAGAGRVD
                     LRSYARERGLEWGPPPGRKVAVVYLSGSIIEGEGVAGSSIGESAAELLAALRDDPTVA
                     GVIMRVDSGGGSALTSDHIARQVKKLKDSGKPVVVSMAGYAASGGYYISAYADRIIAE
                     AGTLTGSIGVTGFDINLTGTLELLGVGSGVVSAGPSGEFGNPFLPRREADGQAERGMI
                     MYIYERFVNVVAEGRGMDRARVDELGKGQIWLGSEAVENGLVDALGGLVDAKAAMEEL
                     LGAAGRFEVYLPGTLEPGSILSLLGLAKAGGASSSMLASARAFVNELAELGEGALYLA
                     P"
BASE COUNT          495 a         1148 c         1168 g          522 t
ORIGIN      
        1 gatcaggtcg taggcgcccc agacgagcat catcccgaag gtccagagcc agaagacgcc
       61 atcggccgcg atggctcgcc aggctggcct cgtcgcggcg cccaagcggt cgagccgctc
      121 gccgacctgc gccgactcca gaggagacgc cgactccaga ggagacgatg actccgaagg
      181 agacgatgac tccgaaggag acgatgactc gggtatggcg agcgctatga acacgccgac
      241 gagcaggttc aggacgccgc tgacgatgaa gccggtccgc ggcgcgacga ggaacagccc
      301 gacgccgatc agcgggcccc agccgaaggc ggcgttgatg gcgatgcggt gcagcgcgta
      361 ggcttccgat cggcgctcgg gcggcgcgaa gtcggcaatc gcggcgttgg tcgccggacg
      421 atagaaggtg cgcgtcatgc cgttcaagaa gttgagtagc gcgagtacgt agacgttcga
      481 cccggcgaac gcgaagccga acaaggccga ggcgctcaag gatacgccga gaatctgcac
      541 cggtttgcgg ccccatcggt cggccaggtg cccgccccag gcgccgaagg cgacactggc
      601 gatcggagcc gcgccgagca cgaacccgag agcgccgagg ctgacgccct cgcccttcat
      661 gtacaggctc aggtacggca ccaccatgaa gcgccccgtg gactcgacca gggtggcggc
      721 gacgaggagc cagatcggct tcgggaagct tgcggcgtag gcggcggcgc ggcgcagcgc
      781 gctcgcggcg gagaaaggct tgcggcgttc gacgggcatg gtgcggcgag tatagcgaca
      841 acgaaacgtg gtcggctatg gtctggcgcg atatggtagt agcgagtttt ggtacgatat
      901 actgtcctcg aggagaccgt tcatgaaccg aaagcatagt cgggcgccat ggcgcttttt
      961 aaccgtcgcg gcgctgttcg tcgtcgcgac cggcgcgttc gcccagtcgg gcggatccgc
     1021 cgacccgcat acggtctggg actatgccga taacccggcc gcctggtcgc tcttccccga
     1081 cgcgatcgcc ttcggcgtgc gggcagaggc cgccgtatcc gatttggcct cgttcgcggg
     1141 cggtaccttg ccggcgactg tcgacctggc ctacggcctg cccggactcg tctatcgcta
     1201 ccgcgacgac ggtagctcgt cgacccacgc cctgacgagc gctttcagct tcggccgctc
     1261 cttcggcctg ggcctgcgct tccgttggga cgggacgacc acgtcggcct ggggcgtcca
     1321 ggacctcggc gtgatgctca ggcccttcgg ctacgcgtcc gtctccgcct cggtcgacga
     1381 cgtgctcgac ctgtccggcg acggctacga agccgcgctc ggcctggcag tccggccgct
     1441 cgccttcaat ccgaggctcg gctccgcgct cacgctgagc gccgacgccc ggctcggccc
     1501 cgacggcttc gctatggccg gcgccggcgc caggttcgtc ctggccgact ggctcagtct
     1561 ctcgggctcg tactcgttcg acgacgcgtc cttcggcctc ggcttcgccg tctcgctgtc
     1621 cggcgccgag acctcggtcg gcgggcggct gaacggcatg catcagggcg gcgacgtcag
     1681 cctgggccag tccgtgcggc tcggccgggc cgccaagtcg gccgagcgcg tcttcgggtc
     1741 gtccgtcctc gtcatcgacg agccgggact gttccagccg aatccgcccc gcgtcgacat
     1801 ggaccccggc ttcggggacg aggtggccct ctggttcgac cttgcggtcg ccgccatcga
     1861 ccgcgccgcc tccgacgcgt cgatccgggc cctggccatg ttcgagccgc ccctgttcga
     1921 taccgacgcg cgcgcccagg aattcggccg cgcgctcggg cgcttccggg cagccggcaa
     1981 gccggtctac gtgttcgccc agtcgatggg ccggctgtca tacgtctacg ccgcggccgg
     2041 cgccgagctc gtcgccctgg acccgaacgg cggcctgccc atcgtcgacg tctcgtcgac
     2101 ccacctatac ttcaaggacc tgctcgccag gctaggcgtc aggacctaca gcctgcagtc
     2161 ccacgatacg aagacggcca acaacgcgat gaccgagccg tccatgacgg gacccgagcg
     2221 cgctatgatc gagcgctacg tcggcggcct ggcgtcgcag gcctgggcct ctctcgacga
     2281 ggcgcgcggc gaccgcgtcg tgggcggcgg cagggccgct atcggcgccg ggccgtacct
     2341 cgatccgcgc aaggccgtcg aggccgggct cgccgacgag ctactgtacc gcgacgagtt
     2401 cgaggagcgg cttaccgagc tgaccgccgg cgcgggccgc gtcgacctgc gttcctacgc
     2461 gcgcgagcgc ggcctcgagt ggggccctcc gcccggcagg aaggtggccg tggtctacct
     2521 gtccggctcc atcatcgagg gcgagggcgt agccggctcg tcgataggcg agtccgccgc
     2581 cgagctactc gccgcgctgc gcgacgaccc gacggtggcc ggcgtgatca tgcgcgtcga
     2641 ctcgggcggc ggctccgcgc tgacgagcga ccacatcgcc aggcaggtca agaagctcaa
     2701 ggactcaggc aagcccgtcg tcgtgtcgat ggccggctac gccgcgtccg ggggctacta
     2761 catctccgcc tacgctgaca ggatcatcgc cgaggcgggt accctgaccg gctcgatagg
     2821 cgttaccggc ttcgatatca acctgaccgg cacgctcgag ctgctcggcg tcggctccgg
     2881 cgtcgtcagc gccggcccga gcggcgagtt cggcaatccc ttcctaccgc gccgcgaggc
     2941 cgacggccaa gccgagcgcg gcatgatcat gtatatctat gagcgcttcg tgaatgtggt
     3001 ggccgagggc cgcggcatgg acagggcccg ggtcgatgaa ctcggcaagg gccagatctg
     3061 gctcggctcg gaggccgtgg agaacgggct cgtcgacgcg ttgggagggc tcgtcgacgc
     3121 caaggccgcg atggaggaac tgctcggcgc ggcgggccgt ttcgaggtct atctgcccgg
     3181 tacgctcgag ccaggctcga tcctgtcgct gctcggcttg gccaaggcgg gcggcgcgtc
     3241 gtcttcgatg ctcgctagcg ctcgagcctt tgtcaacgag ctggcggagc tgggcgaggg
     3301 cgccttgtat ttggctccct agtacctgta ccg
//
LOCUS       DAOOLN010000058         3330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_34719_length_3330_cov_3.429008, whole genome shotgun sequence.
ACCESSION   DAOOLN010000058 DAOOLN010000000
VERSION     DAOOLN010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34719_length_3330_cov_3.429008"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PLW80_01200"
     CDS             complement(<1..597)
                     /locus_tag="PLW80_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPB65147.1"
                     /translation="MRPDTAFRNDARAKVTGRATYADDISIAGMLHAAPVYADYVSAK
                     DLVIDAAEALASPGCVRVITAADVPGSARFGQIEKDYPIFCSGRVRSYGDVCALVVAE
                     TRAAAKAAAALVRVTATPVRPVLTIDEALEPGAPVVPSYGETNVVTHHRLRHGDPESV
                     FAGCELVLEEEFSTQAIEHAYMEPETAVCVPRSDGVVEV"
     gene            complement(601..1497)
                     /locus_tag="PLW80_01205"
     CDS             complement(601..1497)
                     /locus_tag="PLW80_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPB65148.1"
                     /translation="MEGVLDWGVEIIRAIQTVASPGLTAVMRALTLMGSEYFYLIFLP
                     ILFWCVDERYGAHFGLVFLLSSFVNGWLKIVFAQPRPYHLDPAVGLGTESSYGLPSGH
                     AQGTATFWGLLAPKIRRPWGLVLALAVPLVISFTRLYLGLHFPTDLFLGLALGWAFAI
                     AGIVLGDRAARLLASWNVRVRILLAAGIALAMNALNMKDTNLSGIFFGTAVGACFLFD
                     RLRFDASSGGFGQKALRLALGLAGVAAIYFGVKLMAPAWGEPLYALFRFVRYALVGAW
                     VSFGAPWAFMRLGLAGARPAEG"
     gene            complement(1518..>3330)
                     /locus_tag="PLW80_01210"
     CDS             complement(1518..>3330)
                     /locus_tag="PLW80_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPB65149.1"
                     /translation="GIDGAADLLEVVTNAEPLESIPTAPSTADRAPSSAERMSIRRYE
                     ALFKGLVQHASDVVLILESDGRVRFCGPSARSLLGYAPEEIEGRDFFSLVGEGFAAEQ
                     RAMFSSNLVAEHPTFLAGFPLRKADGAELPVEAAASNYLRNPDIVGVIMTLRDVSRQK
                     KAEERALFYEYFDPLTRLPNRESFVRDVDRNVSIAMNRGRVFGVMALGLDRFKMVNDM
                     YGTKTGDEVLKNVAGALRSSFRNDDIVSRYRGDKFLALFPDIKSQDHIKEIIAKARAA
                     FIDPIPIGLGQKLNLSASMGVAFFPNDGKDAAELVRNAETALYMAKESGRDGYRLFDA
                     RLNGEIIERQRIESDLGQAIGERRFEPFFQPKVDRSGFIVGAEALVRWRLPNGETKNP
                     SEFIDVAERSGNIDRIGSMIIMKTCELAASWSAAGLPDIPVSINLSPRQFGQEFLVDD
                     ITAIIESTGVDPRRLEFEITESGIMTNERESIRKLLELKALGAAVSIDDFGTGYSSFS
                     KLKDYPVDTIKMDKSFIDPLPHDKKASIIASAIVDMAHTLSFSVVAEGVETLDQMAFL
                     DTIFCDQYQGYLFSKPVPEDEFRLMLSAGAALLPRSA"
BASE COUNT          561 a         1072 c         1145 g          552 t
ORIGIN      
        1 gacctcgacc acgccgtcgg atcgcggcac gcagacggcc gtctccggct ccatgtacgc
       61 gtgctcgatc gcctgggtgc tgaattcctc ctccagcacg agctcgcagc cggcgaagac
      121 cgactcgggg tcgccatggc gcaggcggtg gtgggtaacg acattcgtct cgccgtagga
      181 cggcacgacc ggcgctccgg gctcgagggc ctcgtcgatg gtcagcaccg gcctgaccgg
      241 cgtggccgtg acccgtacga gggcggcggc ggccttggcg gcggccctcg tctccgcgac
      301 gaccagggcg catacgtcgc cgtaggatcg gacgcggccg gagcagaaga tggggtagtc
      361 cttctcgatc tggccgaagc gcgcggagcc gggcacgtcg gccgcggtga tcacgcgcac
      421 gcagcccggg ctcgccagcg cctccgccgc gtcgatgacg aggtccttgg ccgaaacgta
      481 gtcggcgtag accggcgcgg cgtggagcat gcccgctatc gatatgtcgt cggcgtaggt
      541 ggcgcgcccg gttactttgg cgcgcgcgtc gttgcggaag gcggtatcgg gccgcatcgc
      601 tcagccttcg gccggcctgg cgcccgccag gccgaggcgc atgaaggccc agggggcgcc
      661 gaacgaaacc caggccccga cgagggcgta gcggacgaag cggaacagcg cgtagagcgg
      721 ttcgccccaa gccggcgcca tcagcttgac gccgaagtag atggccgcga ccccggcgag
      781 gccgagggcc agccgtaggg ccttctggcc gaagccgccg gacgaggcgt cgaagcgcaa
      841 tctatcgaat aggaagcagg ctccgacggc cgtgccgaag aagatgccgg acaggttcgt
      901 gtccttcata ttgagcgcgt tcatcgcgag cgcgatgccc gcggcgagga gtatcctgac
      961 gcgcacgttc caggatgcga ggagcctggc cgccctgtcg cccaggacga tgccggctat
     1021 cgcgaaggcc cagccgaggg ccaagcccag gaacaggtcc gtcgggaagt ggagcccgag
     1081 gtacagcctc gtgaagctga tgacgagcgg cacggccagg gccaggacga ggccccaggg
     1141 cctgcgaatc ttcggcgcca ggagccccca gaaggtggcc gtgccctggg cgtgccccga
     1201 aggcaggccg tagctcgact cggtgcccag gccgaccgcc gggtcgaggt gatagggcct
     1261 cggctgggcg aagactatct ttagccagcc attgacgaag ctcgacaaca ggaagaccag
     1321 cccgaaatgc gcgccgtagc gctcgtcgac gcaccagaac aggatgggca ggaagatcag
     1381 gtagaagtac tcggacccca tcagagtcaa cgcgcgcatg accgcggtca ggcccgggct
     1441 cgctacggtc tggatggccc ggataatctc gactccccag tccaagacgc cttccatcgt
     1501 tcgctcctct cttcgatcta cgcgctacgc ggcaacaggg ccgcgcctgc cgacagcatg
     1561 agcctgaatt catcttccgg aacgggcttc gagaataggt agccctggta ctggtcgcag
     1621 aatatcgtgt cgaggaaagc catctggtcc agcgtctcga cgccttcggc cacgacgctg
     1681 aagctcaggg tgtgcgccat gtcgacgatg gccgacgcga tgatcgaggc cttcttgtcg
     1741 tgcggcagcg ggtcgatgaa cgacttgtcc atcttgatcg tgtccacggg gtagtccttg
     1801 agcttcgaga acgacgagta gccggtgccg aagtcgtcta tcgagacggc ggcgccgagg
     1861 gccttgagct cgagcaactt ccgtatgctc tcacgctcgt tggtcatgat gccgctctcg
     1921 gtgatctcga attcgagccg ccgcgggtcg acgccggtgg attctattat tgccgttata
     1981 tcgtcgacca ggaactcctg gccgaactgg cgcggcgaca ggttgatcga aacggggatg
     2041 tccggaagac cggccgctga ccaggaggcc gccagctcgc aggtcttcat gatgatcatc
     2101 gagccgatgc ggtcgatgtt gcccgaccgc tcagccacgt cgatgaattc ggacgggttc
     2161 ttggtctcgc cgttcggcag gcgccagcgc acgagggcct ccgcgccgac gatgaagccc
     2221 gagcggtcga ctttgggttg gaagaacggc tcgaaacgcc gctcgcctat ggcttggccc
     2281 aggtcgctct cgatgcgctg gcgctcgatg atctcgccgt tcagcctcgc gtcgaacaga
     2341 cggtagccgt cgcggccgga ttccttggcc atgtacagcg ccgtctccgc gttgcgcacc
     2401 agctcggcgg cgtccttgcc gtcgttcggg aagaacgcca cgcccatcga cgccgacagg
     2461 ttgagcttct ggcccaggcc gatgggtatg gggtcgatga aggccgcgcg cgccttggct
     2521 atgatctcct tgatgtggtc ctgcgacttg atgtcgggga acagcgccag gaatttgtcg
     2581 ccgcggtagc gcgagacgat gtcgtcgttg cggaacgagg agcgtagcgc gccggccacg
     2641 ttcttgagca cctcgtcgcc ggtcttcgtg ccgtacatgt cgttgaccat cttgaagcgg
     2701 tcgagtccga gcgccatcac gccgaagacc cggccgcggt tcatcgctat cgatacgttc
     2761 cggtcgacgt cgcgcacgaa ggactcgcgg ttcggcaacc tggtcagcgg gtcgaagtac
     2821 tcgtagaaca gcgcccgctc ctcggctttc ttctgcctgg acacgtcgcg cagcgtcatg
     2881 atgacgccga cgatgtcggg gttgcgcagg tagttcgatg cggccgcctc caccggcagc
     2941 tcggcgccgt cggctttgcg tagcgggaag ccggcgagga aggtcgggtg ctcggcgacc
     3001 aggttcgacg agaacatggc tcgctgctcc gcggcgaagc cctcgccgac cagcgagaag
     3061 aagtcccgcc cctcgatctc ctcgggcgcg tagccgagca gcgagcgggc gctcggcccg
     3121 cagaagcgca cgcggccatc ggactcgagt atgagcacca cgtcggaggc gtgctgtacg
     3181 agccccttga acagcgcctc gtagcgccgt atggacatgc gttcggcgct cgacggcgcg
     3241 cgatccgccg tcgacggcgc ggtcgggatg ctttcgagcg gttcggcgtt cgtcacgact
     3301 tcgagtagat ccgcggcgcc gtcgataccc
//
LOCUS       DAOOLN010000059         3296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_35219_length_3296_cov_3.396791, whole genome shotgun sequence.
ACCESSION   DAOOLN010000059 DAOOLN010000000
VERSION     DAOOLN010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35219_length_3296_cov_3.396791"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1512)
                     /locus_tag="PLW80_01215"
     CDS             complement(<1..1512)
                     /locus_tag="PLW80_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPB65150.1"
                     /translation="MRTMARVSTRLAISLATLLAMLLASANACSLLVPYSFEVVAWSP
                     GQERIDEAGGIIVGVEFSREADRPSSESAFSLSVDGEAVLGAFSWLGARMGFTPYAPL
                     RDGADYELRVGTGAMDQAGVSLERAFEARFYTKAERSRPRFVSSDPPDGGRMVGELGT
                     LTLEFDEAIDPIGFRSSLSIAPRARGSWRLVEGGRRIEFRPTEPWARSVDYTCEADAG
                     LRDEEGNEIGRSVVVRFVAGLEGVAPEVTAAAAVDAEGEVVALLEADEAEDGAYTLNR
                     GWESRWRLRMDFSEPVELAGLASALVCDSGPKPLIETIGAYSARVLWRFDEPPEWNGV
                     FTLRVESGIEDQDGDASDSDIVYRIVADGPGSRPPRFVGLRLPLAPGAEAADRELAAF
                     ALDEPFSTLAIAGGDGRYPIGVPTGTSIELYLELAEGASIDLLSTMGAFSFSATNGSL
                     EFDALRVVAGGFDYAEPHPAWSGLDIVRIDGSMTNRADCGLVTFSLDGGYADSW"
     gene            complement(1509..2987)
                     /locus_tag="PLW80_01220"
     CDS             complement(1509..2987)
                     /locus_tag="PLW80_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPB65151.1"
                     /translation="MYRSADEKPQGPPRGAAFIVALCACSLAQSACGLVDLRPVAVAT
                     HPSGPYEVVASRDETLWVMFPAAPSRIDAEEAVTVRSSTGPTDGDLSWDGLCLSWRPI
                     EALDPGSRYRFVLSGDVRMEDGRVADLALDLPFFVLRAGSMPRLVSSAPSDGASVAAS
                     ASGSTALVLRFSEPMDEASVESAFSLSPGPDYRFLWGEGGSLMSIRLLERLSPCVCYA
                     WSLGQDARAVDGAPIARAESGCFVTDLDAETPSVLATGPAALVDGRWVELSTDLSETD
                     AGDSVAVRFSEPMDVDSVVGAITMSPARSGQVVAASTELFVYTPREPWTPEEYQRLVV
                     RTAAKDTSGLALPRERVFGFTPRTPHLELSSVSTLVGEESPSPGEGGAFAVTIGDPPE
                     GELTLILRFSDSFAAVAMESATRSVTVSAFFPSGLRSPRLRSAWWPSADTLSMTWEGL
                     EASDADSERYYRLVVRGGEDGLASQRGLRLRADLAQILEAKP"
     gene            complement(2968..>3296)
                     /locus_tag="PLW80_01225"
     CDS             complement(2968..>3296)
                     /locus_tag="PLW80_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65152.1"
                     /translation="DSGSASFTARVAFVDDPKDLRPGMFARVSVVAGPPTVAVVIPSS
                     SALGEGDQASAFVVSDGVVWRRSLTLGDVGPGGRVVLSGLKVGEVVVDEPGSRLEEGC
                     RVSIGR"
BASE COUNT          588 a         1172 c         1062 g          474 t
ORIGIN      
        1 ccaggaatcg gcgtagccgc catcgagact gaaggtgacg aggccacaat cggcgcggtt
       61 cgtcatcgag ccatcgatgc ggactatgtc cagcccggac caagccgggt gaggctccgc
      121 gtagtcgaaa ccgccggcta ctaccctcag cgcgtcgaac tcgagcgagc cgttcgtcgc
      181 cgaaaatgag aacgcgccca tcgtcgaaag gaggtcgatg ctcgcgccct ctgccagctc
      241 gaggtacagc tcgatcgagg tgcccgtcgg gacgccgatc ggatagcgtc catcgccgcc
      301 agctatcgcc aaggtagaaa acggctcgtc cagcgcgaaa gccgcgagct cgcgatccgc
      361 cgcctcagcc cccggcgcca gcggtagccg aaggccgaca aaacgaggcg gcctcgagcc
      421 gggcccgtcg gccacgatgc ggtacacgat gtcggagtcg gacgcgtcgc cgtcctggtc
      481 ttcgatcccg gactcgactc gcagcgtgaa tacgccgttc cattccggcg gctcgtcgaa
      541 gcgccagagc accctggccg aatacgctcc tatcgtctcg atgagcggtt tcggaccgga
      601 atcgcacacg agagccgagg ccaggccggc tagctcgacc ggttcggaaa aatccatccg
      661 gaggcgccaa cgcgactccc atccgcggtt caaggtatac gcgccgtcct cggcctcgtc
      721 ggcctcgagc agggccacaa cctctccttc ggcgtcgacg gcggcggcgg ccgtcacttc
      781 gggcgcgacg ccttcgagcc cggcgacgaa gcgtacgact accgagcggc cgatttcgtt
      841 gccctcctcg tcgcgcaggc cggcgtccgc ctcgcacgta tagtcgaccg aacgcgccca
      901 gggttcggtc ggcctgaact cgatccgtcg tccgccctcg acgagccgcc aggagccgcg
      961 cgcccgaggc gcgatcgaca ggctggagcg gaacccgatc gggtcgattg cctcgtcgaa
     1021 ttcgagcgtg agcgttccaa gctcgccgac catgcgcccc ccgtccggcg gatcgctcga
     1081 cacgaaccgc ggccttgagc gctcggcctt cgtgtagaag cgggcctcga aggctcgctc
     1141 gagcgatacg ccggcttggt ccatcgcgcc cgtaccgacg cggagctcgt agtcggcgcc
     1201 gtctcgtagc ggcgcatacg gcgtgaagcc catccgtgcg cccagccagg agaacgcacc
     1261 gaggacggcc tcgccgtcga cgctcaacga gaaggcgctt tcagagctag gcctgtcggc
     1321 ctctcggctg aattcgacgc cgacgataat cccgcccgct tcgtcgatcc tctcctgacc
     1381 aggcgaccag gcgacgacct cgaaggagta cggtaccagc agagagcagg cgttggccga
     1441 cgccaggagc atcgccagga gcgtcgccag ggatatcgct aaccgcgtcg atacgcgggc
     1501 catcgttctc atggtttagc ctccaggatc tgcgccaggt cggcccgcag gcgtaggccg
     1561 cgctggctcg caagtccgtc ttctccgccc cgcacgacca ggcggtagta ccgctcgctg
     1621 tcggcgtcgc tcgcctccaa cccctcccag gtcatcgaca gggtatcggc cgacggccac
     1681 caggcgctcc tcaggcgcgg cgaccggagc ccggacggga aaaaggccga taccgtcacc
     1741 gacctcgtag ccgattccat cgcgacggcc gcgaagctgt ccgagaatct gaggatcagt
     1801 gtcagttcgc cttccggcgg gtcgcctata gtcacggcga acgctccccc ttcgcccggc
     1861 gacggactct cctcaccgac tagggtcgag acgctcgata gctcgaggtg cggcgtccgc
     1921 ggcgtgaaac cgaagacgcg ttcccgcggc agggcgagcc cggacgtgtc tttggccgcg
     1981 gtcctcacga cgaggcgctg gtattcctcg ggcgtccaag gctctcgagg ggtatagacg
     2041 aacagctccg ttgacgccgc gacgacctgg ccggaacgag ccggcgacat cgttattgcg
     2101 ccgacgacac tatcgacgtc catgggctcc gagaacctga cggcgaccga gtcgcccgcg
     2161 tccgtctcgg acaggtcagt cgatagctcg acccagcgtc cgtcgacgag agcggcaggc
     2221 ccggtggcga gcaccgaggg cgtctccgca tcgaggtcgg ttacgaagca gccagactcc
     2281 gccctggcta tcggagcgcc atcgacggcc cgcgcgtcct ggcctaagga ccaagcgtag
     2341 cagacgcatg gcgaaagccg ctcgaggagg cggatcgaca tgagcgaccc gccttcgccc
     2401 caaaggaaac ggtagtccgg tcccgggctg agcgagaagg ctgactcgac gctagcctcg
     2461 tccatcggct ccgagaagcg tagcacgagg gccgtggaac cggatgccga cgccgcgaca
     2521 ctcgctccat ccgatggtgc ggaggatacg aggcgaggca tggagccggc tcgcaggacg
     2581 aagaacggaa ggtccagcgc caagtccgcc accctgccgt cttccatgcg cacgtccccc
     2641 gacagcacga aacggtagcg cgatcccgga tcgagcgctt cgatcggtcg ccagctcagg
     2701 cataggccgt cccaggatag gtcgccgtcg gtcggcccgg ttgaggagcg aacggtcaca
     2761 gcctcctcgg cgtctattcg cgacggggca gccgggaaca tgacccacag ggtctcgtcc
     2821 ctcgaggcga cgacttcgta tggtcccgaa gggtgggtcg ctaccgcgac cggcctcaag
     2881 tcgacgagcc cgcaagcgct ctgagcgagc gagcacgcgc agagcgcgac gatgaaggca
     2941 gctccgcgcg gcggcccctg gggcttctca tcggccgatc gatacacggc atccctcctc
     3001 gagcctcgaa cccggctcgt cgactaccac ctcgccgact ttcaggccgg acaacaccac
     3061 gcggccgccg gggccgacgt cgccgagcgt gagcgacctc ctccatacaa cgccgtcgct
     3121 gaccacgaag gcgctcgcct ggtcgccttc gccgagcgcc gacgacgacg ggatgaccac
     3181 cgccacggtc ggcggacccg ctaccacgct tacccgcgcg aacatgccgg gcctcagatc
     3241 cttgggatcg tcgacgaacg cgacgcgcgc cgtgaacgac gcgctgcccg aatcag
//
LOCUS       DAOOLN010000060         3296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_35223_length_3296_cov_3.141314, whole genome shotgun sequence.
ACCESSION   DAOOLN010000060 DAOOLN010000000
VERSION     DAOOLN010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35223_length_3296_cov_3.141314"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1779)
                     /locus_tag="PLW80_01230"
     CDS             complement(<1..1779)
                     /locus_tag="PLW80_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPB65153.1"
                     /translation="MPLPRLTLPVRDYVTKGAGLEDALRASEGQLGTVRVELGAPPSM
                     ADVAAAREHYRKTGMPVAFKAYARPGADSPLEAYSLSVAGDGSPSMLTLLLDEAAAAG
                     QARVSIGGRPALGPSAVGPDRRLVVPITAADSPDAGSGGAVVVELEAGPPESSRTARV
                     AIADASPGEPRVLLATRRTGARSVIEALRPVRTVSYDELGGAGIYRYELIVLDGPDLS
                     SLDGGVAEAIATYVERGAGSLLVAVDSPEVGKPGDCPAIEGLLPVDLSPRALSRLPDV
                     AMAVALDVSGSMYGDKLSLAKAIGLELFANLKATDEAGIILFDEEARWLYPLASVGRL
                     DAERSLAPLRAGGGTRLFPAVAEAIGSLEASGMPEKRLVVVSDGITEPADFDALAARA
                     FRSGIAISAMAVGGEYDKALLTRLSAGSGGRFYRVRDASEVPSLIIEDRKSVSRTVFS
                     ESRVAVVDMAGRPAGHIDGMARLGSRPDALAFYSSAAGDPLLATRRVGSRSTVVYASD
                     LYGRYGREFLENPGTLSLLDALLDGISGESAPALTVTETVDGLALTLAADYLVEPWAA
                     IADGSGRVVANAPFERVAPGHFHAAPG"
     gene            1993..2460
                     /locus_tag="PLW80_01235"
     CDS             1993..2460
                     /locus_tag="PLW80_01235"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HPB65154.1"
                     /translation="MDTNSLRPFFDAAGSVFAQMFGLEPEASAPRALDGAESHDWEVS
                     GLLGIAGSSQGVLALRFTMALVDRLLEASGVESRDEQDRLATASGLVAEMTNIVAGNA
                     IGEIAGQNLEISPPVVIKGKNHQITWPKIAPVVAVDFKTSLGAFELAVCYNGK"
     gene            2729..>3296
                     /gene="hisG"
                     /locus_tag="PLW80_01240"
     CDS             2729..>3296
                     /gene="hisG"
                     /locus_tag="PLW80_01240"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPB65155.1"
                     /translation="MERKDGKLRLALPKGRMQEGVLRLLAEAGLDMRAAERDYRPAAG
                     LDWLDVKVLKPQNIIEMLAAGSRDLGFAGADWVAELGMDLVEVLDTGLDPVRLVAAAP
                     AAMLESGRLPERPLVVASEYEGLARRWIAERGLEAEFVRSYGATEVFPPEDADCIVDN
                     TASGSTLRANGLGVVAEVMSPTTRLTANS"
BASE COUNT          516 a         1133 c         1166 g          481 t
ORIGIN      
        1 gcccggggcc gcgtggaagt gccccggggc cacgcgctcg aacggcgcgt ttgcgacgac
       61 gcggcccgaa ccgtccgcta tggccgccca tggctcgacg aggtagtcgg cggccagcgt
      121 cagcgcgagg ccatcgacgg tctcggtcac ggtgagcgcc ggcgcggact cgccggatat
      181 gccgtcgagt agcgcgtcga gtagcgatag cgttccggga ttctcgagga actcgcggcc
      241 gtagcggccg tagaggtcgc tcgcgtagac gacggtcgac ctggagccga cgcgcctcgt
      301 cgccagcagc gggtcgccgg ccgcgctcga gtagaaggcc agcgcgtccg gcctcgaccc
      361 gagcctggcc atgccgtcga tatggcccgc gggccgcccg gccatgtcga cgacggcgac
      421 gcggctctcg gagaacaccg tcctcgagac gctcttccgg tcctcgatga tcagcgacgg
      481 cacctcggac gcgtccctga cgcggtagaa ccggccgccg gacccggcgg acagccgcgt
      541 cagcagggcc ttgtcgtact cgccgccgac ggccatcgcc gatatcgcga tgcccgaccg
      601 gaaggccctg gccgccagcg cgtcgaagtc ggccggctcg gtgatgccgt cggagacgac
      661 gaccagccgc ttctcgggca tgcccgacgc ctcgagcgag ccgatggcct cggcgacggc
      721 cggaaacagc ctcgtgccgc ctccggcccg gagcggcgcc aggctacgct ccgcgtcgag
      781 ccgtccgacc gacgccagcg ggtacagcca gcgcgcttcc tcgtcgaaca ggatgatgcc
      841 ggcctcgtcc gtcgccttga ggttggcgaa cagctcgagg cctatggcct tggccagcga
      901 cagcttgtcc ccgtacatcg agccggacac atcgagggcc acagccatcg cgacgtcggg
      961 caggcgcgac agcgccctcg gcgacaggtc gaccggcagg agaccctcga tcgccggaca
     1021 gtcgccgggc tttcctacct cgggcgagtc gaccgcgacc aagaggctgc cggcgccgcg
     1081 ctcgacatag gtcgctatcg cctcggcgac gcctccgtcg agcgacgata ggtccgggcc
     1141 gtcgagcacg atcagttcat accgatagat gccggcgccg ccgagttcat cgtagctcac
     1201 cgtacgtacc gggcgcagcg cctcgatcac gctcctggcc cccgtccgcc gcgtggccag
     1261 gaggacgcgg ggctcgccgg gcgaagcgtc ggctattgcg acgcgagccg tcctggagga
     1321 ttcgggcgga ccggcctcca gctcgacgac gaccgcgccg cccgatcccg cgtcgggcga
     1381 gtcagcggcg gttatgggca cgacgagcct gcggtcaggg ccgaccgccg acgggccgag
     1441 cgcgggccgt ccgccgatcg agacgcgggc ctggccggcg gcggccgcct cgtcgagcag
     1501 cagggtcaac atggacggcg agccgtcccc cgcgacggac agggaatagg cctcgagcgg
     1561 cgaatcggcg ccagggcgcg cgtacgcctt gaacgcgacc ggcatgccgg tcttccggta
     1621 gtgttcccgc gccgcggcga cgtcggccat ggacggaggg gcgccgagct cgacgcggac
     1681 cgtgccgagc tggccttcgg acgcgcggag cgcgtcctcg aggcctgccc ccttcgtcac
     1741 gtagtcccgg acaggaagcg tgaggcgcgg taggggcatg ggaagccgga gcgcgtaggg
     1801 gacgagcgtc aggaggggga gagcgatgac gagcccgaac cttacgcgtc tcgcggcgcc
     1861 cgcctgaggg cccgtgtcgt cgcctacctt ttcgcccttg tggttcgcca tggttcgcac
     1921 tattctaata cggtatcggc tggcggacaa ccgaaaccgc gatgctaaaa ccgatcacga
     1981 ggatgcgatt cgatggatac gaatagtttg cggccgttct tcgacgcggc cggctcggtc
     2041 ttcgcgcaga tgttcggcct cgagccggag gcctcggccc cgcgggccct cgacggggcg
     2101 gaatcccatg actgggaagt ttccgggctg ctcggcatag ccggctcgtc gcagggcgtg
     2161 ctagcgctca ggttcaccat ggccctcgtc gacaggcttc tcgaggccag cggcgtcgag
     2221 tcgcgcgacg agcaggacag gctggccacg gcttccggcc tcgtggccga gatgacgaac
     2281 atcgttgccg gcaacgccat cggcgagatc gcggggcaga acctcgagat atcgcccccg
     2341 gtggtcatca agggcaagaa tcatcagata acctggccaa agatagcgcc ggtcgtcgcc
     2401 gtcgacttca agacgagcct cggcgccttc gagctggcgg tatgctataa cggcaagtag
     2461 cctcgatagg cgcttgacgc gggacctccg ttccgtgtag cgtatctccg atgagtaacg
     2521 ctcgatacgt acagttctgc catctccatc atcacggcca cgcctatgcg gtcgcgatga
     2581 tcgtcatacc ctaggttcca gtatcggaac cgaacggaac gatacgccgg ccgcccgagg
     2641 ccggcgtttt cgtttcttcg gggcttccgg gcctccgcga gacggacgcg gccgcgcgcg
     2701 gccttctatc tcggaaggag cgaacgacat ggaacggaag gacgggaagc tgcgcctggc
     2761 gctgcccaag ggaaggatgc aggagggcgt gctgcgcctc ctcgcggagg cgggcctgga
     2821 catgagggcc gccgagcggg actacaggcc ggcggccggc ctcgactggc tcgacgtcaa
     2881 ggtgctcaag ccccagaaca tcatcgagat gctcgccgcc ggcagccgcg acctcggttt
     2941 cgccggggcc gactgggtgg ccgagctcgg tatggatctc gtcgaggtgc tcgataccgg
     3001 tctcgacccg gtgcgcctcg tcgccgcggc ccccgcggcc atgctcgaat cgggccgact
     3061 gcccgagcgc cctctcgtgg tcgccagcga gtacgagggc ttggctcggc gatggatagc
     3121 ggagcggggc ctggaggcgg agttcgtgcg ctcctacggg gccaccgagg tcttccctcc
     3181 ggaggatgcg gactgcatcg tcgacaatac ggcgtccggc tcgacgctgc gcgccaacgg
     3241 cttgggcgtc gtcgccgagg tgatgagccc gacgacgcgg ctcaccgcca acagcc
//
LOCUS       DAOOLN010000061         3296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_35226_length_3296_cov_2.096267, whole genome shotgun sequence.
ACCESSION   DAOOLN010000061 DAOOLN010000000
VERSION     DAOOLN010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35226_length_3296_cov_2.096267"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLW80_01245"
     CDS             complement(<1..242)
                     /locus_tag="PLW80_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB65156.1"
                     /translation="MLRIDDLSVHYGGIHALQGISFEVPEGKIVTLIGANGAGKSTTL
                     KSVVGLVKPSGGSISLDGERVSGLATRDIVRRGVVLA"
     gene            complement(242..973)
                     /locus_tag="PLW80_01250"
     CDS             complement(242..973)
                     /locus_tag="PLW80_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB65157.1"
                     /translation="MRFGGLTAVSDLNIEIQKNEIVGLIGPNGAGKTTAFNAITGVYR
                     PTEGRVVFKGRDITGQKPHAITKLGIARTFQNIRLWKDMDVLENVLVACHLRMDVGVF
                     ASTLHLPGYRVKERRSREFAMSLLESVGLADQAREAATSLPYGKQRRLEIVRALATEP
                     SLLLLDEPAAGMNPQESLELMDFIGSIRDRYDLTILLIEHHMQVVMGVCTRMYVLDYG
                     VTIAHGTPDEIRRDPKVIEAYLGVD"
     gene            complement(994..2052)
                     /locus_tag="PLW80_01255"
     CDS             complement(994..2052)
                     /locus_tag="PLW80_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB65158.1"
                     /translation="MKIKRNTLLTLAGLALLVGAIAAADATLGRFSVRILNLSAIYIV
                     LALSLNLLNGFTGLFSLGHAGFMAVGAYVSALLTMSPAQKDMNFFLEPIVPFLRDIQL
                     PFPLALLAAGTCAGLVAVVIGVPALRLRDDYLAIATLGFAEIIRVLITNAQSLTNGAQ
                     GLKAITKYSTTWVVWSVAALSVVFMLALVRSSYGRTMKAVRDDEVAAEAMGVNVFKVK
                     STSFVISSFMAGVGGALLGHMITTIDPKMFTLTLTFNILLIVVLGGNGSITGSVIGAI
                     VVTVLMEALRFLDMPMNFLFIKTNGLPGLRMVIFSILLMIVVLYRQRGLMGNKEFDWD
                     GFIGIFRRKGASSAGGRS"
     gene            complement(2057..2950)
                     /locus_tag="PLW80_01260"
     CDS             complement(2057..2950)
                     /locus_tag="PLW80_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB65159.1"
                     /translation="MELSTFFQQLTNALSLGSLYALIAIGYTMVYGILRLINFAHGDV
                     FMLGAYFAFYAATSLAMPWWVAFIVAIGLTAIFGMGLERVAYRPLRDSPKISVMISAI
                     GASFLIENVGIVVFGGRPKSFPSIPLFDTVVHLGPVSVLSVSLFIPVITAAILVALLW
                     IVNGTKTGLAMRAVSTDLDAARLMAIDVNRTISLTFGIGSLLAGLGGIMWTMKYPQLN
                     PLMGVMPGLKCFIAAVIGGIGNIPGAVLGGFLLGVLEIMIVAFLPELTGYRDAFAFVL
                     LIVVLLVKPSGLLGKHQAVKV"
     gene            complement(3088..>3296)
                     /locus_tag="PLW80_01265"
     CDS             complement(3088..>3296)
                     /locus_tag="PLW80_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HPB65160.1"
                     /translation="DGYLLAIDAIKRAGGIDPLKIRDAIAATKDFAGAAGYVTLDANG
                     DPIKSAVIKEVKGGKFVFKTTVAP"
BASE COUNT          598 a         1030 c         1098 g          570 t
ORIGIN      
        1 gcgagtacca cgccgcggcg cacgatgtcc ctggtcgcca gcccggatac gcgctctccg
       61 tccaggctga tcgatccacc gctcggcttg accaggccga cgacggactt gagcgtcgtg
      121 ctcttgccag cgccgttcgc gccgatcagc gtgacgatct tgccctccgg cacttcgaac
      181 gagatgccct gcagggcgtg gatgccgccg taatgtaccg acaggtcgtc tatcctgagc
      241 atcagtccac ccccagatag gcttcgatga ccttgggatc gcggcggatc tcgtcgggag
      301 tcccgtgggc tatcgtcacg ccgtagtcga ggacgtacat gcgcgtgcag acgcccatca
      361 ccacctgcat atggtgctcg atcaacagta tggtcaggtc gtagcggtcc ctgatggagc
      421 cgatgaagtc catgagttcg agcgactcct gcgggttcat gcccgctgcc ggctcgtcga
      481 gcaggaggag cgaaggctcg gtggccagcg cccgcactat ttcaagccgg cgctgcttcc
      541 cgtatggtag cgacgtggcc gcctcgcgcg cctggtcggc caatccgacc gactcgagca
      601 gcgacatggc gaactcgcgc gagcgtcgtt ccttgacgcg gtagccgggc agatgcagcg
      661 tcgaggcgaa caccccgacg tccatcctga ggtggcaggc gacgagcacg ttctcgagca
      721 cgtccatgtc cttccagagc cgtatgttct ggaaggtcct cgcgatgccg agcttggtga
      781 tcgcgtgggg cttctggccg gtgatgtcgc ggcccttgaa gaccacccgc ccctcggtcg
      841 gcctgtagac gccggtgatg gcgttgaagg cggtggtctt gcccgcgccg ttcgggccga
      901 tgaggccgac gatctcgttc ttctggatct cgatgttcag gtcggacacg gcggtcaggc
      961 cgccaaagcg catcgagacg tccctggtgc ttatcacgag cggcctcccg cgctcgacgc
     1021 gcccttccgc cggaagatcc cgatgaagcc gtcccaatcg aattccttgt tacccatgag
     1081 cccccgctgc cggtatagca cgacgatcat cagcagtatc gagaagatga ccatgcggag
     1141 ccccggtagc ccgttcgtct tgatgaacag gaaattcatg ggcatgtcca ggaatcggag
     1201 cgcctccatc agcacggtga cgacgatggc tcctatcacc gagccggtga tcgagccgtt
     1261 gccgccgagc acgacgatca gcaggatgtt gaaggtgagc gtcagcgtga acatcttggg
     1321 gtcgatggtt gttatcatgt gcccgagcag ggcgccgccg acgccggcca tgaagctgga
     1381 gatgacgaag ctcgtcgact tgaccttgaa cacgttgacg cccatggcct cggcggccac
     1441 ctcgtcgtcc cgcaccgcct tcatcgtgcg gccgtagctc gagcgcacga gggccagcat
     1501 gaacacgacc gatagggccg ctaccgacca gaccacccag gtcgtcgagt acttggtgat
     1561 ggccttgagg ccctgggcac cgttcgtcag gctctgcgcg ttcgtgatga gcacacggat
     1621 gatctcggcg aagccgagcg tcgcgatggc caggtagtcg tccctgagcc ggagcgccgg
     1681 tacgccgatg acgacggcga ccaggccggc gcaggtcccg gcggccagga gggccaacgg
     1741 gaacggcaac tggatgtcgc gcaggaaggg cacgatgggc tccaggaaga aattcatgtc
     1801 cttctgcgcc gggctcatcg tcaggagcgc gcttacgtac gcgccgaccg ccatgaagcc
     1861 ggcgtggccg agcgagaaga ggcccgtgaa cccgttgagc aggttgagcg acagggctag
     1921 cacgatatag atggctgaga ggttgagtat cctgaccgag aacctgccga gcgtcgcgtc
     1981 ggccgcggcg atggctccga ccaaaagggc gaggcccgcg agggttagta gcgtgttccg
     2041 cttgatcttc atagtgtcac accttgaccg cttgatgctt gccgagcagg cccgacggct
     2101 tgacgaggag tacgacgatc agtagcacga acgcgaacgc gtcgcggtag ccggtcagct
     2161 ccggcaggaa cgccacgatc atgatctcga gcacgccgag caggaagccg ccgagcacgg
     2221 cgccggggat gttgcctatg ccgccgatca cggcggcgat gaagcacttg agcccgggca
     2281 tcactcccat cagcgggttc agctgggggt acttcatcgt ccacatgatg ccgccgaggc
     2341 cggccagcag cgagccgatg ccgaaggtca gcgagatcgt cctgttcacg tcgatggcca
     2401 tcagcctggc cgcgtccagg tcggtggaga cggcgcgcat ggccagcccg gtcttcgtac
     2461 cgttgactat ccagagtagc gcgacgagta tggcagccgt gatgaccggg atgaacaggc
     2521 tgaccgacag caccgacacc gggccgaggt gtaccaccgt gtcgaacagc gggatgctcg
     2581 ggaagctctt ggggcggccg ccgaacacga cgatgccgac gttctcgatc aggaaggacg
     2641 cgccgatcgc gctgatcatc accgagatct tcggcgagtc acggagcggc cgatacgcga
     2701 cccgctccag gcccatgccg aatatcgccg tcaggccgat cgccacgatg aaggctaccc
     2761 accacggcat cgcgagcgaa gtcgcggcgt agaacgcgaa gtacgcgccg agcatgaaga
     2821 cgtcgccatg ggcgaagttt atcaggcgta ggataccgta gaccatcgtg tagcctatcg
     2881 ctatgagggc gtagaggcta ccgaggctca acgcgttggt cagttgctgg aagaaagtcg
     2941 atagttccat tggttcgggt cctcgggcgc cgcgatcgcg gctgtaaacg aggacgggcg
     3001 cctccgtcgg cgtggacggc tgcgcccgtc gcgcgtccgg ccgtcgcgaa gcggcggacc
     3061 ggctacgcta tctatggatg gcggccgtca aggggctacg gtcgtcttga acacgaactt
     3121 gccgcccttc acttccttga tgacggcgct cttgatcggg tcgccgttgg cgtcgagggt
     3181 cacgtagcca gcggcgccgg cgaagtcctt ggtggccgcg atggcgtcgc ggatcttgag
     3241 cgggtcgatg ccgccggcgc gcttgatcgc gtcgatcgcg agcaggtagc cgtcga
//
LOCUS       DAOOLN010000062         3288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_35338_length_3288_cov_2.395917, whole genome shotgun sequence.
ACCESSION   DAOOLN010000062 DAOOLN010000000
VERSION     DAOOLN010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35338_length_3288_cov_2.395917"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PLW80_01270"
     CDS             <1..717
                     /locus_tag="PLW80_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65161.1"
                     /translation="NKILLTSHVYQRLKGAGVSIQQVDGINKVDFFNTGAIEFKGISL
                     TVYDTVFLDTEGYRLGYREAMENLYVAIDKGMWKSSIFETAATLAEAIIDNLPGGEKG
                     GASRRSMLDDLKAATDSFGVERYEKAVASFGELVRKLEALPGMDGLALEYLRGVHDNY
                     TALVDAFTARLDRDIDEHLDTVFGQKELERFRLYRQHHALYGELREQTRLQARSRKVI
                     WFREADRISPELSVRIMARK"
     gene            755..1447
                     /gene="rsmG"
                     /locus_tag="PLW80_01275"
     CDS             755..1447
                     /gene="rsmG"
                     /locus_tag="PLW80_01275"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPB65162.1"
                     /translation="MNETLLTRGLAELGLADDRTASLLGRYVASIEAWNPAYGLVGAS
                     GDELVIKHILDSLAPLAIVRRSLAEAATGTAGYGERASLVDLGTGAGLPGVPLAIAMP
                     EVDVTLLDRMTRRIRFLEAVRAELALENVDIVEEQVERAKGSYDVVTFRAFRPFERKL
                     FKRVFSVCSPGGRVVAYKGKADRARAELEAIDGLYESAEITPVVVPFLADERCVVVMR
                     PKASPTASPRKP"
     gene            complement(1460..1774)
                     /locus_tag="PLW80_01280"
     CDS             complement(1460..1774)
                     /locus_tag="PLW80_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65163.1"
                     /translation="MEIPARRASTSDRIPTASGTNVAFTVTNGTGRSGAFTVLFFVTD
                     SKTYKLRQIHEERVKLDAGEAFGLPPYRFDAPGKYKLVVKDSWGNPLVEREFMVTERE
                     DT"
     gene            complement(1846..2385)
                     /locus_tag="PLW80_01285"
     CDS             complement(1846..2385)
                     /locus_tag="PLW80_01285"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPB65164.1"
                     /translation="MDMKRIGCIVALALLAGSAFAQSSEDEARRRLVDAAFKYRGVPY
                     VYGAESPRAFDCSGFVRYVYREALGLELPRSARSFWSSGSPIDWRKARAGDVLVYDTV
                     GGAPSHVALVVGDGTVIHAVSAGTKTGVIVSPMSDRYWAPRLLGARSFIVASTETVSA
                     APVAPASPRLRRPRRRQRR"
     gene            2597..>3288
                     /locus_tag="PLW80_01290"
     CDS             2597..>3288
                     /locus_tag="PLW80_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65165.1"
                     /translation="MKRFVCLAMIATTLSIGAFGQSYAALPTGSVGSADPQFALSILT
                     DYFEKHERSSRTGGVVLSSIGSALLAGGLAGVGYSFAAPSTSFSDPEGQMLMRGLSIG
                     VSGAGLIVGGIGIGILAKPDDAYKREYAYLYAETDPVVQEAIAFGIMKELADDAKRGR
                     IVGGIVNVATPLAAAGGHAIAAAATGDWSDFDDRVIGTMSWTLPSLVSGIIVLVSGRS
                     TEERMLDSYRAM"
BASE COUNT          587 a         1067 c         1089 g          545 t
ORIGIN      
        1 aacaaaatac tcctgaccag ccacgtctac cagcggctca agggcgcggg cgtctcgatc
       61 cagcaggtcg acgggatcaa caaggtggac ttcttcaata ccggcgccat cgagttcaag
      121 ggcatcagcc tgacggtcta cgacacggtc tttctcgata ccgagggcta caggctcggc
      181 taccgcgagg cgatggaaaa cctgtacgta gccatcgaca agggcatgtg gaagtcgagc
      241 atcttcgaga cggccgcgac cctggcggag gccatcatcg acaacctgcc cggaggggag
      301 aagggcggcg cctcgaggcg ctcgatgctc gacgacctca aggccgccac cgatagcttc
      361 ggggtagagc gctacgagaa agcggtcgcg tcgttcggcg aactggtgcg caagctcgaa
      421 gcgttacccg ggatggacgg attggcgctc gagtacctgc ggggggtaca cgataattat
      481 acggcgctcg tcgacgcgtt caccgccagg ctcgaccgcg atatcgacga gcacctcgac
      541 acggtcttcg ggcagaagga gctggaacgc ttcaggctat accgccagca ccacgccctg
      601 tacggggagc tccgggagca gaccaggctg caggcgcgct cccgcaaggt catctggttc
      661 cgcgaggccg ataggatctc gcctgagctg agcgtccgca tcatggccag gaagtaacta
      721 tgcgggcggc cctctttcag gctacattcg agccgtgaac gaaacgctgc ttacccgggg
      781 gctggccgag ctcggcctcg cggacgaccg gaccgcgagc ctgctcggtc gctacgtcgc
      841 ctcgatcgag gcctggaacc ccgcctacgg cctcgtcgga gcctcgggcg acgagctcgt
      901 catcaagcat atcctcgaca gcctggcgcc gttggccatc gtcaggcgat cgctcgccga
      961 ggcggcgacc ggaaccgcgg gctacggcga gcgggccagt ctcgtcgacc tcgggaccgg
     1021 ggccggcctg cccggcgtgc cgctggccat agcgatgccg gaggtcgacg tgaccctgct
     1081 cgaccgcatg accaggcgca tccgtttcct ggaggcggtc cgcgccgagc tagcgcttga
     1141 gaacgtcgac atcgtcgagg agcaggtcga gcgcgccaag ggttcttacg atgtagtcac
     1201 cttcagggcc ttcaggcctt tcgagcggaa gctgttcaag cgcgtcttct cggtctgctc
     1261 gcccggcggc cgcgtcgtcg cgtacaaggg caaggccgac cgcgcccgcg ccgagctcga
     1321 ggcgatagac gggctctacg agagcgcaga aataacgccg gtcgtcgttc cgttcctggc
     1381 cgacgagcgc tgcgtcgtcg tcatgcggcc aaaggctagc ccaacggcta gcccgcgcaa
     1441 gccctaggcc gactaccgac tacgtatcct cacgctccgt aaccatgaac tcccgctcga
     1501 cgagcgggtt gccccacgag tccttgacca cgagcttgta cttgccgggc gcgtcgaaac
     1561 ggtacggcgg taggccgaag gcctcgcccg cgtcgagctt gactcgttct tcgtggatct
     1621 gccggagctt gtaggtcttg gagtcggtga cgaagaacag tacggtgaaa gcgccagagc
     1681 ggccggtgcc gttcgtcacg gtgaacgcga cgttcgtgcc ggaggccgtc gggatgcggt
     1741 ccgaggtcga agcccgcctg gccgggatct ccacgcctat atcggcgatg acctgttcgg
     1801 ggacacttgg cccggccttc ggagcggctt taggtgctac tggcgctacc ggcgctgccg
     1861 gcgtcgcggc cgccgcagcc ttggcgaggc gggtgctacc ggcgctgctg acacggtctc
     1921 cgtcgacgca acgatgaacg agcgggcgcc gagcaggcgt ggagcccagt agcggtcgct
     1981 catcggcgag acgatgacgc cggtcttcgt cccggccgat acggcgtgga tgacggtgcc
     2041 gtcgccgacg accagcgcga cgtggctcgg ggcaccgccg accgtgtcgt agaccagcac
     2101 gtcgccggcc cgggccttgc gccagtctat gggcgagccg ctcgaccaga acgaccgcgc
     2161 gctacgcggc agctcgaggc cgagcgcttc gcgatacacg tagcggacaa agcccgagca
     2221 gtcgaaggcc cggggggact cggcgccgta gacgtacggt accccccggt acttgaaagc
     2281 ggcgtcgacg agcctgcgcc tggcctcatc ctcagaactc tgagcgaagg ccgaacccgc
     2341 gagcagcgcc agggccacga tacacccgat tcgtttcata tccataccac gactatccgt
     2401 cggcctcgcc cgctcgtcaa gcgcgaggcg tctccatgtg ctatgctatc gaatgtgata
     2461 gcgatgaact atcgattctg atatgagtga ggagcggcaa gcttgtagaa cggccttcga
     2521 gctataccat aaccatggca cgcttattgc agtattaccg ctaggcgccc gagtatcgag
     2581 cgccgggagg ttgaccatga aacgattcgt ctgtctagcg atgattgcca cgacgctgtc
     2641 gatcggagcc ttcggccagt cctacgccgc gctaccgacc ggttcggtcg gctcggccga
     2701 cccgcagttc gccttatcca tactgaccga ctacttcgag aaacacgagc gctcgagcag
     2761 gaccggcggc gtcgtgctgt cgagcatagg cagcgccctg ctcgccgggg gcctggcggg
     2821 cgtcggctat tcgttcgccg caccgtcgac gagcttctcc gatcccgagg gtcagatgct
     2881 gatgcgcggt ctgtcgatag gcgtctcggg ggccgggttg atcgtcggcg gcatcggcat
     2941 cgggatcttg gccaaaccgg atgacgcgta caagcgcgaa tacgcctatc tgtacgccga
     3001 gaccgacccg gtcgtacagg aggcgatagc gttcggtatc atgaaggaac tggccgacga
     3061 cgcgaagcgc ggcaggatag tcggcggtat cgtgaacgtc gccacccccc tcgccgccgc
     3121 cggcgggcac gcgatcgcag cggccgccac cggcgactgg agcgatttcg acgacagggt
     3181 catcggtacg atgagttgga ccctgccgag cctcgtctcg ggcatcatcg tgctcgtctc
     3241 cggacgctcg accgaggagc gcatgctcga ctcgtaccgc gcgatgag
//
LOCUS       DAOOLN010000063         3257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_35821_length_3257_cov_2.783573, whole genome shotgun sequence.
ACCESSION   DAOOLN010000063 DAOOLN010000000
VERSION     DAOOLN010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35821_length_3257_cov_2.783573"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PLW80_01295"
     CDS             <1..508
                     /locus_tag="PLW80_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent RNA helicase"
                     /protein_id="HPB65166.1"
                     /translation="PEKKRKKALLVWDAFRRARENLPADAGLAYKGMLGVVIVGHDRL
                     MDGEKLETILRVAHWLDPEADLKRDWPRKKQFNVNEDLDALVSALDHVLQVSYWKTKN
                     PELGFVAMFTDTAGGYWFRVSRGFHTALNESLASLEALVDEVSELASPEQKERVGSLY
                     RRLSEFFA"
     gene            complement(593..1306)
                     /gene="trmB"
                     /locus_tag="PLW80_01300"
     CDS             complement(593..1306)
                     /gene="trmB"
                     /locus_tag="PLW80_01300"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HPB65167.1"
                     /translation="MESKAAGLTGPLSSRGDGIHSYVIRAGRMSDVQKDAMIRLYPVY
                     GFAWDPERTLGPADILGREAPFVVEVGFGMGQATADIAEALPDTAFLGFEVHAPGVGR
                     LLAEIEARGIRNLRVSRHDAVEAIAAVLAPGSVDGFHVFFPDPWPKKRHHKRRIMNAA
                     FVRLMSSRLKPGGYIYYATDWEEYAEATLETLLAEPTLENAHERWAERRSWRPVTKFE
                     RRAIEDGRPIRELEFRRRT"
     gene            complement(1282..1917)
                     /locus_tag="PLW80_01305"
     CDS             complement(1282..1917)
                     /locus_tag="PLW80_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65168.1"
                     /translation="MALALAVSCSSSPPEIVSLAVRLIVVTTDPGGARDERLSVFASV
                     ADAQGVGDVEYLYVVHDGDELCWTMGPSEWRRSDDGSSVWLGANSLDAPGTTIPRGSY
                     RLILIDKAGERAERSFSLSAPETSAYDTPSLRLSGTTVVVATPYPTNTAFFLDAGGNV
                     THTAAIAKGSTALDSLWPEGKWRTEADYIAVYGLEPKAEIGFFSWKARQPD"
     gene            complement(1967..3256)
                     /gene="hisS"
                     /locus_tag="PLW80_01310"
     CDS             complement(1967..3256)
                     /gene="hisS"
                     /locus_tag="PLW80_01310"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPB65169.1"
                     /translation="MIQPRVLKGFRDSLPRAEIERAAMIEALTGAFRSFGFVPIDTPV
                     LEYAEILLGKAGGETEKQVYRFADNGGRDVAMRFDLTVPFARFMAEHSGELYLPFRRY
                     HMAKVWRGENTQRGRYREFMQCDFDIVGVDTASADFDIVATIARSMTELGVPGATIRV
                     NHRGIFNRFLERAGVADRSVEALRVVDKLAKIGEPETRRQLTELAGVAAADEIMAFIA
                     GGEAFGPTLDRMTRLAGGPNPDSDRLSEIFSMAEACGLSSSLELCPSITRGLDYYTGV
                     VFETFLRELPGIGSVCSGGRYNDLASLYTKQRLPGVGASVGIDRLIAALEELGRSGAK
                     AGYTRVLVANVEPALTPEYQAAAAVLRDAGIACEVYPEPRKLALQYAYAEQKGASYAL
                     IRGSDDKARGLWLLRDLAARSNAEHADLGSVIAALGD"
BASE COUNT          553 a         1073 c         1095 g          536 t
ORIGIN      
        1 ccccgagaag aagcgcaaga aggccttact cgtctgggac gccttcaggc gggctaggga
       61 gaacctgcct gcggacgcgg gactggccta taagggtatg ctcggcgtcg ttattgtcgg
      121 ccacgaccgc ctgatggacg gcgagaagct agagaccatc ctgcgcgtcg cccattggct
      181 cgacccggag gctgacctca agcgggactg gccgcgtaag aagcagttta acgtgaacga
      241 agacctggac gccctggtct cggccctcga ccacgtgctc caggtatcgt actggaagac
      301 gaagaacccc gagctgggct tcgtcgcgat gttcaccgac accgccggag gctactggtt
      361 cagggtatcg cgcggcttcc atacggccct gaacgagagt ctggcgagcc tcgaggctct
      421 ggtcgacgag gtctcggagc tggcgagccc cgagcagaag gagcgcgtcg gatccctgta
      481 ccgcaggttg tcggagttct tcgcgtaggc ggcgccaacg ctacgtgcgg cgccaacgct
      541 acgtgcggcg ccgacgctac gtgcggcgcc gacgctacgt gcggcgccga cgctacgtgc
      601 gtcgcctgaa ctccagctca cgtatcggcc gcccgtcctc gatggcccgg cgctcgaatt
      661 tagttacggg cctccagctc ctgcgctcgg cccatcgttc gtgcgcgttc tcgagggtcg
      721 gctcggcgag gagcgtctcg agcgtcgcct cggcgtattc ctcccaatcg gtcgcgtagt
      781 agatatagcc gcccggcttg agtcggctcg acatgagccg gacgaaagcc gcgttcatga
      841 tgcgccgctt gtggtgccgc ttcttgggcc atgggtcggg gaagaacaca tggaatccat
      901 cgaccgagcc gggagccagg accgcggcta tcgcctcgac cgcgtcgtgc ctggacacgc
      961 gtaggttacg tatgccgcgg gcctctatct cggccaggag cctgccgaca cccggcgcat
     1021 gtacctcgaa accgaggaag gccgtatcgg gcagggcctc ggctatgtcg gcggtcgcct
     1081 ggcccatgcc gaagccgacc tcgacgacga aaggcgcctc gcgtcccagt atgtccgcgg
     1141 gtccgagcgt gcgctcgggg tcccacgcaa agccgtagac ggggtagagc cggatcatcg
     1201 cgtccttctg gacatcgctc atgcggccgg ctcggatgac gtagctgtgt ataccgtccc
     1261 ctcgggacga tagcgggccg gtcagtccgg ctgccttgct ttccatgaga agaatcctat
     1321 ctccgccttc ggctcgaggc cgtagacggc aatgtagtcg gcctcggtac gccatttccc
     1381 ctctggccag agcgaatcaa gcgcggtaga gcctttggcg atggcggcgg tgtgcgtcac
     1441 gttgccgccc gcgtcgagga aaaaagccgt attcgtcgga tagggcgtgg cgacgacgac
     1501 cgtggtgccc gacaggcgca gcgacggcgt atcataggcg ctcgtctcag gcgcgctgag
     1561 cgagaagctc cgctcggcgc gctcaccagc cttgtcgatc aggataaggc gataggagcc
     1621 gcgcggtatc gtcgttccag gggcgtcgag cgaattcgcc ccgagccaga cgctcgagcc
     1681 gtcgtccgag cggcgccatt cggacggccc catcgtccag cacagctcgt cgccgtcgtg
     1741 gacgacgtac aggtactcga cgtcgccgac gccctgggcg tcggcgaccg aggcgaacac
     1801 cgacaggcgc tcgtccctgg cgccgcccgg atcggtcgtc acgacgatca gccgaaccgc
     1861 caacgatacg atctccggtg gcgacgagga gcaggagacg gccagcgcca gcgccacggc
     1921 tgacgacaac gtcgccgcga gggtcggtcc gagccgccgc aagcgctcag tcccccagcg
     1981 cggcgatcac cgagcccagg tcggcgtgct cggcgttcga cctggccgcc aggtcgcgga
     2041 gcagccacag cccacgtgcc ttatcgtcgg aaccgcgtat caacgcgtac gacgcgccct
     2101 tctgctcggc gtaggcgtac tgcagggcca gcttgcgcgg ttcgggatac acctcgcagg
     2161 caattccggc gtcgcggagc acggcggcgg cggcctgata ctctggcgtc agggccggct
     2221 cgacgttggc gacgaggacg cgggtataac ccgccttggc gcctgaacgt ccgagctcct
     2281 cgagggcggc tatcaggcgg tcgatgccta cggaggcgcc gacgccgggc aggcgctgct
     2341 tcgtgtacag cgaggcaaga tcgttgtaac ggccgcccga acagaccgag cctatgccgg
     2401 gcaactcgcg gaggaaggtc tcaaagacga caccggtata gtagtcgagg ccccgggtga
     2461 tgcttggaca cagttctagc gagcttgaga gcccgcaggc ctcggccatc gagaaaatct
     2521 cggacagacg atcggagtcg gggttcgggc cgcctgccag tcgcgtcatg cgatcgaggg
     2581 tggggccgaa ggcctcgccg ccggcgatga aggccatgat ctcgtctgcc gcggcgacgc
     2641 cggccagctc cgtcagctga cgccgcgtct caggctcgcc tatcttggcc aacttgtcga
     2701 ctacgcgtag cgcctcgacg gacctgtccg cgacgccggc acgctccagg aagcggttga
     2761 atataccgcg gtggttcacg cggatggtcg cgccgggcac gccgagctcc gtcatcgatc
     2821 gggcgatggt cgccacgatg tcgaagtccg ccgaggcggt gtcgacgccc acgatatcga
     2881 aatcgcactg catgaactcg cgataccgcc cgcgctgcgt gttctcgccg cgccagacct
     2941 tggccatgtg gtagcgcctg aaaggcaggt acagctcgcc cgagtgttcc gccatgaagc
     3001 gggcgaaggg gacggtcagg tcgaagcgca tcgccacgtc gcgccctccg ttatcagcga
     3061 agcggtacac ctgcttctcc gtctcgccgc cggccttacc gagcagtatc tcggcgtact
     3121 cgaggacggg cgtgtcgatc ggcacgaagc cgaagctcct aaaggcgccg gtgagcgcct
     3181 cgatcatcgc ggcccgctcg atctcggctc gcggaagcga gtccctgaag cccttgagca
     3241 ctcgaggctg tattatt
//
LOCUS       DAOOLN010000064         3246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_35980_length_3246_cov_3.002194, whole genome shotgun sequence.
ACCESSION   DAOOLN010000064 DAOOLN010000000
VERSION     DAOOLN010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35980_length_3246_cov_3.002194"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="PLW80_01315"
     CDS             complement(<1..1356)
                     /locus_tag="PLW80_01315"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPB65170.1"
                     /translation="MRTQNVLEMRGICKSFPGVRALHDVSFTLRAGEVHALMGENGAG
                     KSTLIKVMTGVYEKDSGQIAIEGNAIHFKSPQDAQAFGVGTVYQEITLCPNLTVAENM
                     FIGRGRYAFVNWKRMRERAARLLDSLGIPARPTQQLSSCSLAIQQMIAIARAIDMDCK
                     ILILDEPTSSLDEDEVRKLFSLMRELKSRGVGIVFVTHFLDQVYEISDRITVLRNGEF
                     IGEYEASALPQFELVSKMMGKALDDVARIRKHEPSTRDGETPVFEATGLSSAAGVRPF
                     DFSIRKGEVNGFTGLLGSGRSESVRAIYAADKVTGGDIKIKGKKANIGEPIHAMRLGI
                     GYLPEDRKGDGIIADLSVRDNIILALQVLHGFWKPFSRKQAEAFADEYIRSLNIKTAS
                     SATPIKSLSGGNQQKAILARWLLTHPEYMILDEPTRGIDVGTKVEIQKLVLKLAEEGV
                     SV"
     gene            complement(1463..2467)
                     /locus_tag="PLW80_01320"
     CDS             complement(1463..2467)
                     /locus_tag="PLW80_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPB65171.1"
                     /translation="MSSQSRLTKVFCTALVLLVAFAGAVSAQGSLIRVGIVNLPPEES
                     GYRQANVEDMNAVFSRANGYDMKQTNTGDNSEQIAAAQGYIRDGVKYLLISAANASGW
                     DNVLKAAKTAGVTVFLFDRLIQTDASNYKAALVSDMAAEGTTAVNWLLSQKLPAYNVI
                     LIRGQMGSAAELGRSSALLKARDSGKVKIVADGTGGDSWSPEEARKVVEAAIAAGKDF
                     NVIYAQNDGMAQGAVQALDAAGISHGKNGKVKIIGFDFNRFALRNVQAGYWDCDIQCS
                     PRQAAEISGWIKTLESGKKSPTGIVYQKEIYADTATITDELILSKGINADPGKGVITK
                     "
     gene            2913..>3246
                     /locus_tag="PLW80_01325"
     CDS             2913..>3246
                     /locus_tag="PLW80_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPB65172.1"
                     /translation="MDQNETARELLALNMKRLRAAVGISQMVLAQRVGCSTTLIGNIE
                     IQKRFPSAENLDRIAGSFGVPVYELFIRDSPAIDRVASNFEVKESIERKVLGPSTACS
                     TRKGSTTSD"
BASE COUNT          571 a          989 c          968 g          718 t
ORIGIN      
        1 cacgctgacc ccttcctcgg cgagcttgag caccagcttc tggatctcca ccttggtccc
       61 gacgtcgata ccccgcgtcg gctcgtcgag tatcatgtac tcgggatggg tcaacagcca
      121 tcgggcgagg atggccttct gctggttgcc cccagagagc gatttgatcg gcgtggcgct
      181 ggacgcggtc tttatgttca ggctccggat gtactcgtcc gcgaacgcct cggcctgctt
      241 cctcgagaac ggcttccaga acccgtgcag gacctgcagc gccaggatga tgttgtctcg
      301 cacggagagg tcagcgatga tgccatcgcc cttgcgatcc tccggcaggt agccgatgcc
      361 cagccgcatc gcgtggatgg gctccccgat gttcgccttt ttgcccttga tcttgatgtc
      421 gccgccggta accttatcgg ccgcgtagat cgcgcggacg ctctcgctgc gcccggagcc
      481 gagcaaaccg gtgaacccgt tcacttcgcc tttccgtatg gagaagtcga agggcctgac
      541 ccccgccgcg ctcgatagcc ccgtcgcctc gaacaccggc gtctcgccgt cgcgagtcga
      601 aggctcatgc ttccgtatcc tcgcgacgtc gtcgagcgcc ttacccatca tcttcgatac
      661 cagctcgaac tggggcaggg ccgacgcctc gtactcgcct atgaattcgc cgttgcgcag
      721 caccgtgatc ctgtcgctga tctcgtatac ctggtctagg aagtgggtca cgaagacgat
      781 gcccacgccc ctggacttca gctcgcgcat cagcgagaag agcttgcgaa cctcgtcctc
      841 gtcgagcgac gaagtcggct cgtccaggat gaggattttg cagtccatgt cgatggcgcg
      901 ggcgatcgct atcatctgct ggatggccag cgagcagctc gacagctgct gggtcggcct
      961 ggccgggatg ccgagcgagt ccaggagcct ggcggcccgt tccctcatcc gtttccaatt
     1021 gacgaaggcg tagcggcccc tgcctatgaa catgttctcc gcgacagtca ggtttgggca
     1081 gagtgtaatc tcctggtaca cggtgccgac cccgaaggcc tgagcatcct ggggcgactt
     1141 aaaatggatg gcgtttcctt ctatcgctat ctggccggag tccttctcgt agacgccggt
     1201 catcaccttg atcaaggtgg acttgccggc gccgttctcc cccatgaggg cgtgaacctc
     1261 tcccgcccga agggtgaagc tgacgtcgtg cagggccctg accccgggaa acgacttgca
     1321 tatgccccgc atctcaagca cattctgcgt tcgcactcgt tactctccct gatgcatcga
     1381 gaataaaaac gtgcggcgcg gcaagtctga ctatacccag cgccgcgccg cacgcctcaa
     1441 gcggttctat gactatatac ggttacttcg tgatcacgcc cttgcctgga tcggcattga
     1501 tgcccttgct caggatgagc tcgtcggtga tcgtggccgt atcggcgtag atctctttct
     1561 gatagacgat gccggtgggg ctcttcttgc cggattccag ggtcttgatc caaccggaga
     1621 tctcggccgc ctggcggggg ctgcactgga tgtcgcaatc ccagtagccg gcctgtacgt
     1681 tgcgtagggc gaagcggttg aaatcgaagc cgatgatctt gaccttgccg ttcttgccgt
     1741 ggctgatgcc ggcggcgtcg agggcctgga ccgcgccctg ggccataccg tcgttctgcg
     1801 cgtagatgac gttgaagtcc ttgccggcgg ctatcgcggc ctcgactacc ttgcgggcct
     1861 cttcggggct ccagctgtcg ccgccggtcc cgtccgcgac gatcttgacc ttgcctgagt
     1921 cgcgggcctt gagcagggcg ctgctacggc caagctcggc cgctgaaccc atctgccccc
     1981 ggatgaggat cacattgtag gcggggagct tctggctcag aagccagttg acggcggtgg
     2041 ttccctcggc ggccatatcg gagacgaggg cggccttata gttcgaggcg tcggtctgga
     2101 tgaggcgatc gaagaggaat acggttacgc cggcggtctt ggcagccttc agtacgttat
     2161 cccagccgga cgcgttcgcc gccgagatca gtaggtactt gactccgtcg cggatatagc
     2221 cctgggccgc cgcgatctgc tcgctattgt cgccggtgtt ggtctgcttc atgtcatagc
     2281 cgttggccct cgagaagacg gcgttcatgt cctcgacgtt ggcctggcga tagccggatt
     2341 cctcgggcgg caggttcacg attcccaccc ggatcaggga accctgggcg ctgaccgcgc
     2401 cggcgaaagc gaccaggagt actagggccg tacagaatac tttcgttaac cgagattgag
     2461 atgacatttc atgcctccga tactacggaa caacttcacg ttctctggcg gcaacgccgc
     2521 gcattcgctt gactttcgaa gccctcgggt agtgacaacg ctgaaaccgg aatcctgaga
     2581 ccgctcgctc ctccttccat cgtcgatacc gagtcggacg attctgtccg ctcatcggga
     2641 ttgggtgtat gtacgaaaca ctctgttctc tgtagcgtgt acgtacacgg tgctactcgt
     2701 atggtactat cattcgtatt tctgtcaatc tttttttagt tcggataggt ttttcggcgg
     2761 taccgcgcac gcagtcctta tcgatcttgg cttccggcga atgcttcgat gtacgaagat
     2821 ggcctaatag aaataaacta ataccgccaa gggatgattg aggcatggct tttccggggt
     2881 gacactatag cacccgggag gagctcaacg ctttggacca gaacgaaacc gctcgagagt
     2941 tgctcgccct taacatgaag cggctcaggg ccgctgtcgg gatatcgcag atggttctgg
     3001 cgcagcgggt cggctgctcc accacgctca taggcaatat agagatacag aagcggtttc
     3061 cctcggccga gaacctggat cgtatagccg gctccttcgg cgttccggtc tacgagctgt
     3121 tcatccggga ctccccggct atcgataggg tcgcttcgaa cttcgaggtc aaggaaagca
     3181 tcgagcgcaa ggtgctcggg ccatccacgg cgtgctcgac gagaaagggt tctactacca
     3241 gtgacg
//
LOCUS       DAOOLN010000065         3229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_36228_length_3229_cov_3.186830, whole genome shotgun sequence.
ACCESSION   DAOOLN010000065 DAOOLN010000000
VERSION     DAOOLN010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36228_length_3229_cov_3.186830"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2212)
                     /locus_tag="PLW80_01330"
     CDS             complement(<1..2212)
                     /locus_tag="PLW80_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HPB65173.1"
                     /translation="MFDDMSPDDLEYVGKKAGRPDMLEKLTGEATFVSDMSLPGMLHV
                     QLKKSPYARARIKGVDVSKAAAMPGVRAVVTGDELDYHIGLYIVDKYILAKGEVRHYG
                     EAVAAVAAETIEQARAAVEAIEVDYEPLAPVLHHMDALKPDAPLVHPDLGTYSYIEAV
                     FTPKPGTNIANHTKLRKGDVEKGFAESTYVVEREYSCPSVQHVPMETHVAIVQWGRGD
                     KVSIWTSAQSPFTVRNLFCHAFGLPHINVRVQIPYVGGGFGGKAGIGVEPLCAVLSRK
                     AGGKPVKFQASREEEFSLLPCRSALTYRIKTGVSAEGKILAQHMTMYWDSGAYADYAV
                     NVTRASGYSASGPYEIPNAWIDAYTIYTNKPYGTAYRGFGHVEFHWAVERHMELVATA
                     IGMDPLAFRLKNALRPGSVTLTGEKIVEHTGDVVKCIEEAARRVGYGQLTEAEKAREK
                     QTGWKIGKGVAALQKAPAMPSYTATAVVLKMNEDGSVLVNLALTDYGQGTYASIRQIV
                     AERLGFPIEKIKVAFENDTDRDPYDWQTVASKGLLLSGNAAILAAEDLLKNAYAVAAE
                     VLHANPRDLAHDGTRVFVRHRADQCVSFASMAVGFALPNGSGVGGPLIGVGRYIAQGL
                     SNLNKETGEGNPALDWTFGAHGMVVEVNEETGEYRVLKIASIYDVGRAINPDIVRGQA
                     IGGMMQGLGTAICEGYIYDQQGRLLNPSFTDNKIPTAKDLPLEVECAVVETPQVD"
     gene            complement(2205..2690)
                     /locus_tag="PLW80_01335"
     CDS             complement(2205..2690)
                     /locus_tag="PLW80_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPB65174.1"
                     /translation="MRIKLTVNGLIYERDCIEHKTLLRFLREDLGLMGTKEGCGMGEC
                     GACTVIMNGRAVNSCMVLAVEADGASIRTIEGEAEGPSLSDIQRAFERNHAVQCGFCT
                     AGMVMSVKELLAEHPKPSADQVKEAIEGNFCRCTGYTQIVEAVLDLTGSPEKKGELEH
                     V"
     gene            complement(2692..>3229)
                     /locus_tag="PLW80_01340"
     CDS             complement(2692..>3229)
                     /locus_tag="PLW80_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HPB65175.1"
                     /translation="LCDCAHELASHQIRNRATVAGNVVNASPCSDMAPALLCLGAKAT
                     IRSATGERVVPFSQFFAGVKKTVLAPGEFVSCIMVPAEAAGARGRYLKLKRIAGHDLG
                     IVGVLMTRRDGELRFAVSSAAPTPVLVDSVTEKDSADEAVRKVLAAVSPISDVRGTKE
                     YREHMIGVYARRLFTEVE"
BASE COUNT          492 a         1055 c         1068 g          614 t
ORIGIN      
        1 cgtccacctg cggcgtctcg accacggcgc actccacctc gagcggcaga tccttggccg
       61 tcggtatctt gttgtcggtg aaggacgggt tgaggaggcg gccctgctgg tcgtagatgt
      121 agccctcgca gatcgcggtg ccgaggccct gcatcatgcc tccaatcgcc tgcccgcgga
      181 cgatgtccgg gttgatcgcg cggccgacgt cgtagatgct ggctatcttg agcacgcggt
      241 actcgccggt ctcctcgttc acctcgacga ccatgccgtg ggctccgaag gtccagtcga
      301 gggccgggtt gccctcgccg gtctccttgt tcaggttgga caggccctgg gcgatgtagc
      361 ggccgacgcc gatcagcggt ccgccgacgc ccgaaccgtt cggcagagcg aagccgaccg
      421 ccatcgacgc gaaactgaca cactggtcgg cgcggtgcct cacgaagacc ctcgtcccgt
      481 catgggccag gtcgcgcgga ttggcgtgga gtacctcggc ggccaccgcg taggcgttct
      541 tgagcaggtc ctcggcggcc aggatggcgg cgttgcccga cagtagcagg cccttggacg
      601 cgactgtctg ccagtcgtag gggtcgcggt ccgtgtcgtt ctcgaaggct accttgatct
      661 tctcgatcgg gaagccaagc cgctccgcga cgatctgccg tatcgaggcg taggtgccct
      721 ggccgtagtc ggtcagggcc aggttgacga ggaccgagcc gtcctcgttc atcttgagca
      781 cgaccgcggt ggccgtatac gacggcatcg ccggcgcttt ctgtagcgcc gcgacgccct
      841 tgcctatctt ccagccggtc tgcttctcgc gggccttctc tgcctcggtc agctggccgt
      901 agccgacgcg gcgcgccgcc tcctcgatgc actttaccac gtcgccggtg tgctcgacga
      961 tcttctcccc ggtcagggtg acggagcccg gcctcagcgc gttcttcagc cggaacgcga
     1021 gcgggtccat gccgatcgcg gtcgccacca gctccatatg gcgctcgacg gcccaatgga
     1081 attcgacgtg gccgaagcca cggtaggcgg taccgtaggg tttgttcgtg tagatcgtgt
     1141 aggcgtcgat ccatgcgttc ggtatttcgt atggaccgga cgccgagtag ccggaggccc
     1201 tcgtcacgtt gacggcgtag tccgcatagg cacccgagtc ccagtacatg gtcatgtgct
     1261 gggcgaggat cttgccctcg gcggacacgc cggtcttgat acggtaggtg agcgctgaac
     1321 ggcagggcag gaggctgaac tcctcctcgc ggctggcctg gaacttgaca ggcttgccgc
     1381 ccgccttgcg cgacagtacg gcgcagagtg gctcgacgcc gatgccggcc ttcccgccga
     1441 agccgccgcc gacgtagggg atctgcacgc gcacgttgat gtgcggtagc ccgaaggcgt
     1501 ggcagaacag gttccgcacg gtgaacggcg actgggcgct cgtccagatc gataccttgt
     1561 ctccccggcc ccactgcacg atggctacgt gcgtctccat cggcacgtgc tgcaccgacg
     1621 ggcaggaata ctccctctcg acgacgtagg tcgactcggc gaagcccttc tcgacgtcgc
     1681 ctttgcgcag cttggtatgg ttggcgatgt tcgtgcccgg cttgggcgtg aagacggcct
     1741 cgatgtacga gtacgtgccc aggtccggat ggaccagcgg cgcgtccggc ttgagcgcgt
     1801 ccatgtggtg gagcaccggg gccaggggct cgtagtcgac ctcgatcgcc tcgaccgccg
     1861 cccgggcctg ctcgatggtc tcggcggcga cggccgccac ggcttcgccg tagtgcctga
     1921 cctcgccctt ggccaggata tacttgtcga cgatatacag gccgatgtgg tagtcgagct
     1981 cgtcgccggt gacgacggcc cggacgcccg gcatcgccgc ggccttggag acgtctacgc
     2041 ccttgatgcg cgcgcgcgcg taggggctct tcttgagctg cacgtggagc atacccggca
     2101 gagacatgtc gctgacgaag gtggcctccc cggtcagctt ctcgagcatg tccggccgtc
     2161 cggccttctt gccgacgtac tcgaggtcgt cggggctcat atcgtcaaac atgctcgagc
     2221 tctcccttct tctcggggct acccgtaagg tcgagcaccg cctcaacgat ctgggtataa
     2281 ccggtacacc ggcagaagtt gccctcgatg gcctccttga cctggtcggc gctcggcttc
     2341 gggtgttcgg cgagcaactc cttgaccgac ataaccatgc cggccgtgca gaagccgcac
     2401 tggacggcgt ggttgcgttc gaaagcccgc tgtatatcgg agagcgacgg tccctcggcc
     2461 tcgccttcta tcgtccgtat cgacgccccg tccgcctcga cggcgagcac catgcaggag
     2521 ttgaccgctc ggccgttcat gatgacggtg caggcgccgc actcgcccat gccgcagcct
     2581 tccttggtgc ccatgagacc gagatcctcg cggaggaaac ggagcagcgt tttgtgctct
     2641 atgcagtcgc gctcgtagat gagcccgttg accgtaagct tgattctcat atcactcaac
     2701 ctccgtgaac aggcgccggg catagacgcc tatcatgtgc tcgcggtact ccttggtgcc
     2761 gcgaacgtcg ctgatcggcg atacagcggc gagcaccttg cgtacggcct cgtccgccga
     2821 gtctttctcc gtgaccgagt cgacgaggac cggcgtcggg gcggccgagc tgacggcgaa
     2881 gcgtagctcg ccgtcgcgcc tggtcataag gacgccgacg atgccgaggt cgtggccggc
     2941 gatgcgcttg agcttgagat agcggccgcg agccccggcg gcttctgccg gaaccatgat
     3001 gcacgatacg aattcgcccg gcgcgagcac ggtcttcttg acgcccgcga agaactgcga
     3061 gaatggcacg acgcgctcgc cggtagccga gcgtatcgtc gccttggctc ccaggcagag
     3121 cagggcggga gccatgtcgg agcatggcga ggcgttgacc acattaccag ccacggttgc
     3181 gcggttgcgt atctggtggc tggcgagctc gtgagcgcag tcgcataga
//
LOCUS       DAOOLN010000066         3227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_36258_length_3227_cov_3.174653, whole genome shotgun sequence.
ACCESSION   DAOOLN010000066 DAOOLN010000000
VERSION     DAOOLN010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36258_length_3227_cov_3.174653"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            376..1236
                     /locus_tag="PLW80_01345"
     CDS             376..1236
                     /locus_tag="PLW80_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024752187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HPB65176.1"
                     /translation="MIINHNISAMYANRQLGVNGLAVDKGMEKLSSGMRINRAGDDAS
                     GLAVSEKMRSQIRGLNQASKNAANGVSFIQTTEGYLQESQDILQRLRELSIQAANGIY
                     TAEDRMQIQVEVSQLVDEIDRIASHAQFNGMNLLTGRFAREDGENLVTGSMWFHIGAN
                     MDQRERVYIGTMTAYGLGVKQIGSDEIVSLESPDGANRTIGVLDEALKKVNKQRADLG
                     AYQNRLEHAVKGIDIGAENLQAAESRIRDTDMASEMVHYTKDRILVQASSAMLAQANQ
                     KTASVLQLLQ"
     gene            1491..2339
                     /locus_tag="PLW80_01350"
     CDS             1491..2339
                     /locus_tag="PLW80_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HPB65177.1"
                     /translation="MIINHNMSAMYANRSLGVTGVELNKNMEKLASGQRINRAGDDAS
                     GLAVSEKLRGQVRGLNQAQRNIENGVSFIQTTEGYLQETQDILHRVRELAVQSANGIY
                     TAEDRMQIQVEVSQLVDEINRIASHAQFNGMNILTGRFSKDGGNIMQLQVGANMDQNE
                     RIFIGTMTATALGLQGAQGTDGMISIEDPEKANASIGNLDAALKTVSKQRADLGAYQN
                     RFEMASKGVAIAAENLQAAESRIRDTDMASTMVDYVKNQILSQAGGAMLAQANTRTQS
                     VMQLLG"
     gene            2517..2849
                     /locus_tag="PLW80_01355"
     CDS             2517..2849
                     /locus_tag="PLW80_01355"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HPB65178.1"
                     /translation="MAIDVSAVYTLPNAASALQQEAAKPRAKQGDVMRTEPPVSMDTI
                     IEELVDVSAAFNKRLSFSLNEKLGQVVVKVIDTDTDKVIREIPPAELQHVNERIREVI
                     GILFDERA"
     gene            2875..>3227
                     /locus_tag="PLW80_01360"
     CDS             2875..>3227
                     /locus_tag="PLW80_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
                     /protein_id="HPB65179.1"
                     /translation="MSDISIPGVKSKYDTAKIIEGLMAVERIPLTRSQARLEEIRLQR
                     TVWLDLNRRMSSLRESARSLFSFQNPFNERTASSSDESVLTGTATRPAIEETKSFVVK
                     RTASADRFMSDAVPA"
BASE COUNT          718 a         1038 c          972 g          499 t
ORIGIN      
        1 cgtcgtcggc gatgaacgag aattcttcgg ttttcatgcc tggcagtata cacccggggc
       61 cgcgcctacg caaaaagaat acttcgggcg gatggacgat tcgctagcag cgatccatag
      121 ccaaagctct cgataggtaa aaataggact caaggaatcc cgcgcccatg ccgatatata
      181 aagaggagtt gggctttagg cgtttcggtc catcaggggc cgtcgcgttc ctaccctact
      241 ctacgataat cggccccggg atagataact tgagcgtcgc cgaagggcga aaggaggagg
      301 aggcgaccta gaccggccac gccgtatcga gacgcggaac acggatgctc cgcgcacaga
      361 cacaaggagg aagtcatgat catcaaccac aacatcagcg cgatgtacgc caaccgccag
      421 ctcggcgtca acggcctcgc cgtcgacaag ggcatggaga agctgtcttc gggcatgcgc
      481 atcaaccgcg ccggcgacga cgcttccggg ctagccgtat ccgagaagat gcggtcccag
      541 atccgcggcc tgaaccaggc ctcgaagaac gcggccaacg gcgtgtcgtt catccagacg
      601 accgagggct acctgcagga gagccaggac atcctgcagc gcctcaggga gctgtcgatc
      661 caggcggcca acggcatcta caccgccgag gaccgcatgc agatccaggt cgaggtatcc
      721 cagctcgtcg acgagatcga ccgcatcgcc agccacgccc agttcaacgg catgaaccta
      781 ctgaccggcc gcttcgcccg cgaggacggt gagaacctgg tcaccggctc tatgtggttc
      841 cacatcggcg ccaacatgga ccagcgtgag cgcgtgtaca tcggcacgat gaccgcctat
      901 ggcctcggcg tcaagcagat cggctcggac gagatcgtat cgctcgagtc gcccgacggc
      961 gcgaaccgga ccatcggcgt cctcgacgag gcgctcaaga aggtcaataa gcagcgagcg
     1021 gacctcggcg cttaccagaa taggctcgaa cacgccgtca agggcatcga catcggcgcg
     1081 gagaacctcc aggccgccga gagccgcatc cgcgacacgg acatggcgtc ggagatggtc
     1141 cactacacga aggaccgtat cctcgtccag gcctcgagcg cgatgctcgc ccaggcgaac
     1201 cagaagaccg cttccgtctt gcagttgctc cagtaaggct gtatactaaa aaaagatagg
     1261 gccgtcccct cccgaccggg aaagggacgg ccgacggatc tttgacaaac accctcgtgc
     1321 gcatgccggc ggagatgccg gccctcccct cagggagggc cggttccccc gccgtatggg
     1381 gcatgaacgg acgggaacgg gcgcgagaac acggaatccc ggcctcgacg ccatgcgcgc
     1441 gggacgcgcg gcaaggaatg ccgcgcagta cgtcctgcaa ggagggtcat atgatcatta
     1501 accacaacat gagcgcgatg tacgccaaca ggtcgctggg cgttaccggg gtcgaactca
     1561 acaagaacat ggagaagctc gcctccggtc agcgcatcaa ccgcgccggc gacgacgcct
     1621 cgggcctcgc ggtttccgag aagctccgcg gccaggttcg cggcctcaac caggctcagc
     1681 gcaacatcga gaacggcgtt tcattcatac agaccacgga gggttacctc caggagacgc
     1741 aggacatcct gcatcgcgtg cgcgaactgg ccgtccagtc ggccaacggc atctataccg
     1801 ccgaagaccg catgcagatc caggtcgagg tttcccagct ggtcgacgag atcaaccgga
     1861 tcgccagcca cgctcagttc aacggcatga acatcctgac cggccgcttc tcaaaggacg
     1921 gcgggaatat catgcagctg caggtcggtg ccaacatgga tcagaacgag aggatcttca
     1981 tcggcaccat gaccgccacg gctctcgggc tacagggcgc tcagggtacg gacggtatga
     2041 tctccatcga ggatccggag aaggccaacg cctccatcgg caacctcgac gccgccctca
     2101 agacggtttc gaagcagcgc gccgacctcg gcgcctacca gaaccgcttc gagatggctt
     2161 ccaagggcgt cgcgatagcc gccgagaacc tccaggccgc cgagagccgc atccgcgaca
     2221 ccgacatggc ctcgacgatg gtcgattacg tcaagaacca gatcctgtcc caggccggtg
     2281 gggcgatgct cgcccaggcc aataccagga cccagtccgt gatgcagctg ttgggttaat
     2341 acaaccgttc atagattcat tgcggagagc ttcctggacg tcgtctttcc gcgacggagt
     2401 ctgatctgac cggaaggggg gggatcgcgc cctcccccct tcatctttac gaaccgggcg
     2461 cgcccccgcg acagggagtc ggaacgggag cgctcccatg agacaggagg aaacgtatgg
     2521 caatcgacgt ttctgccgta tatacgctcc ccaacgccgc gtcggccttg cagcaggaag
     2581 ccgctaagcc gcgcgccaag cagggcgacg tgatgaggac cgagcctccc gtatcgatgg
     2641 ataccatcat cgaggagctc gtggacgtca gcgccgcgtt caacaagcgg ctatcgttca
     2701 gcctgaacga gaagctcggc caagtcgtcg tcaaggtaat cgataccgat accgacaagg
     2761 taattaggga aattccgccc gccgagcttc agcacgtaaa cgagcgtata cgggaggtaa
     2821 tcggcatcct gttcgacgag agggcgtaac ccggaaggag ggcctcgagg ccccatgtcg
     2881 gatatcagca tccccggcgt caagagcaag tacgataccg ccaagataat cgaaggcttg
     2941 atggccgtcg agcgcattcc gctcacgcgc tcccaggccc gcctcgagga gatacgcctc
     3001 cagcgtacgg tctggctcga cctcaaccgc cgcatgtcgt cgctccggga gagcgctcgt
     3061 tcgctcttct ccttccagaa ccccttcaac gaacgcacgg ccagctcgag cgacgagtcg
     3121 gtattgaccg gcaccgccac caggccggcg atcgaggaga cgaagtcctt cgtcgtcaag
     3181 cggacggcga gcgccgaccg tttcatgtcg gacgccgtac ccgccga
//
LOCUS       DAOOLN010000067         3225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_36288_length_3225_cov_2.780126, whole genome shotgun sequence.
ACCESSION   DAOOLN010000067 DAOOLN010000000
VERSION     DAOOLN010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36288_length_3225_cov_2.780126"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PLW80_01365"
     CDS             <1..639
                     /locus_tag="PLW80_01365"
                     /inference="COORDINATES: protein motif:HMM:NF016230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPB65180.1"
                     /translation="LNTALSMAQGITYFSGVRPCTYEEDFIVEKSERVANLVHAAGIQ
                     SPGLASAPAIAEDVAAMVVELASRFRRVTPRAGWRAGRPAPREPRRLGLEARDALITG
                     NPAYGRVVCRCEEVSEGEIVDAIRSIVPATDLDAIKRRTRAGMGRCHGGFCTPRVMEL
                     VARETGLPAWAVTKKGPGSELCVGPTKAGPPRFSADAAHADAAHADAGGIEP"
     gene            636..1985
                     /locus_tag="PLW80_01370"
     CDS             636..1985
                     /locus_tag="PLW80_01370"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB65181.1"
                     /translation="MIRRFETDVVVLGAGPAGLAAACAAMEAGARVVVVEREERTGGI
                     LKQCIHDGFGLARYGRQLSGPEYALRDADRFGALGGLAMLEAFVTSAVRRDGGGFELA
                     CVDRGGACLLRSTALVLATGCRERTDRQVFVHGDRPAGVFTAGLAQYMINVQGLLPGR
                     RAVILGSGDIGLIMARRMTLEGMEVLGAYEAKPEPSGLSRNLRQCLDDYGIPLTLSST
                     VTAVHGERRVEGVTTALVDGSMRPIPGTERDLSCDTLVLSVGLIPENELAKSLGVPLD
                     PRTRGPRVDQNMHTLVDGVFACGNAVHVNDLADYVSESGERAGSAAAAYARSGPGSAG
                     RKRRLSPLVAGAGCLYVVPQLLDLDALDRAAGTRDDLCVYFRSNRTMPVGATARLAVG
                     DGPGGAALLERRYRALRPPEMERIKAATGALAAPLASQAEPGRGASLTIEPDGVEDR"
     gene            1982..2353
                     /locus_tag="PLW80_01375"
     CDS             1982..2353
                     /locus_tag="PLW80_01375"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HPB65182.1"
                     /translation="MSERKELTCIECPRGCRLTVEVDGARAMVSGNACPKGAVWGAQE
                     AVSPMRVLTTTVAVDGSARKRLPVRSDGVLPLGRLLDAMAAIDPIVVRPPFRRGDVVV
                     RDLLGLGVDLVATDDLLGDGR"
     gene            2350..>3225
                     /locus_tag="PLW80_01380"
     CDS             2350..>3225
                     /locus_tag="PLW80_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPB65183.1"
                     /translation="MSAASSADLVLAIDCGTQSLRALAFDGRGALVARRKVAYEPYFS
                     SRPGWAEQDPELWWRSLVAASSGLVAELPGGADRIAALAVTTQRDTIVCLDGDLRPLR
                     PAILWLDARRAPAPYHPSALVALGVSLVGMREALQKTQEAGKCNWIMANEPGLWERTR
                     ACVLVSGFLNARLSGELADSTASQIGHLPFDYRRQAWAAPGSRNARMFPIAPEKLPRL
                     APAGTELGRLSARAAAELGLPAGLPVIAAGSDKGCETIGSGVVDETAASLSFGTTATV
                     QTTSQRYREPIAFMPA"
BASE COUNT          423 a         1178 c         1166 g          458 t
ORIGIN      
        1 ctcaatacgg cgctatcgat ggcccagggc atcacctatt tctcgggcgt gcgcccctgc
       61 acctacgagg aggacttcat agtcgagaag agcgagcgcg tcgcgaacct cgtgcacgcg
      121 gccggcatcc agtcgccggg gctggcctcg gcgccggcca tagccgagga cgtggcggcg
      181 atggtcgtcg agctggcgag tcgcttcagg cgcgtgacgc ctagagccgg ctggcgggcg
      241 gggcggcccg cgcctcgcga accgaggcgc ctcggcctcg aggcccgcga cgcgttgata
      301 accgggaacc cggcttacgg ccgcgtcgtc tgccgctgcg aggaggtctc cgagggcgag
      361 atcgtggacg cgatacgctc gatcgtgccg gccaccgacc tggatgcgat caaacgccga
      421 acgcgggcgg gcatgggccg ctgccacggc ggcttctgca cgccgcgggt catggagctc
      481 gtggcgcgag agacggggct gccggcctgg gccgttacca agaagggccc gggctcggag
      541 ctgtgcgtcg gcccgaccaa ggccggcccg ccgcgcttct ctgccgacgc cgcgcacgcc
      601 gatgccgcgc acgccgacgc cggcgggata gagccgtgat ccggcgtttc gagaccgacg
      661 tcgtggtgct cggcgccggc ccagccggcc tggccgcggc ctgcgccgcg atggaggccg
      721 gcgcccgcgt cgtcgtcgta gagcgcgagg agcggaccgg cggcatcctc aagcagtgca
      781 tccacgacgg tttcggcctg gcgcgctacg gccgccagct gtccgggccc gagtacgccc
      841 tgcgcgacgc cgaccgcttc ggcgcgctcg gcggcctggc catgctcgag gccttcgtca
      901 cgtcggcggt tcgcagggac ggcggcggct tcgagctggc ctgcgtcgac cgcggcggcg
      961 cctgcctgct acggtccacg gccctcgtgc tggcgaccgg ttgccgcgag cgcaccgacc
     1021 gccaagtctt cgtgcacggc gaccggccgg ccggcgtgtt caccgcgggc ctggcccagt
     1081 acatgatcaa cgtgcagggc ctcctgcccg gccgccgcgc cgtgatcctc ggctccggag
     1141 acatcggcct gatcatggcc cgccgcatga ccctcgaggg catggaggtg cttggcgcct
     1201 acgaggccaa accggagccc tcgggcttgt cgcgcaacct gcgccagtgc ctcgatgact
     1261 acggcatccc gctgaccctg tcgtccaccg tgacggcggt tcacggcgag cgccgcgtcg
     1321 agggcgtaac gacggccctc gtcgacggct cgatgcggcc gatacccggc acggagcgcg
     1381 acctgagctg cgacaccctc gtgctgtcgg tcggactgat acccgagaac gagctggcca
     1441 agagcctcgg tgtgccgctc gacccgcgga cgcgcggccc gcgggtcgac cagaacatgc
     1501 acaccctcgt cgacggggtg ttcgcctgcg gtaacgcggt ccatgtgaac gacctggccg
     1561 actacgtctc cgagtcgggc gagcgggccg gctcggccgc ggcggcgtac gcgcgctccg
     1621 gccccggctc ggcgggccgt aagcgccgcc tgtcgccgct cgtcgcgggg gccggctgcc
     1681 tctacgtcgt gccgcagctc ctggacctgg acgcgctcga tcgcgccgcc ggaactcgcg
     1741 acgacctatg cgtctacttc cgctcgaacc ggaccatgcc agtcggcgcg accgcgagac
     1801 tggccgtcgg ggacgggccc ggcggggctg cgctactcga gcggcggtac cgcgccttgc
     1861 gcccgcccga gatggagcgt atcaaggcgg ccactggcgc cctggccgcc cctctggcta
     1921 gtcaggcgga gccggggcgc ggcgccagcc taacgatcga accagatgga gtggaggacc
     1981 gatgagcgag cgcaaggaac tgacctgtat cgaatgccct cgcggctgcc ggctgacggt
     2041 cgaggtggac ggcgctcgcg cgatggtgag cggcaacgcc tgccccaagg gtgccgtttg
     2101 gggcgctcag gaggccgtgt cccctatgcg cgtcctgacc acgaccgtcg cggtcgacgg
     2161 ctcggccagg aagcgccttc cggtgcgctc cgatggggtc cttcctctcg gccgcctcct
     2221 cgacgcgatg gccgccatcg accccatcgt cgtccgtccg ccgttccgtc gcggcgacgt
     2281 cgtcgttcgc gacctcctcg gcctcggcgt cgacctcgtc gccaccgacg acctcctcgg
     2341 ggacggacga tgagcgccgc gtcctcggcc gacctggttc tggccattga ctgcggcacc
     2401 cagagccttc gggcgctggc cttcgacggg cgtggcgcgc tcgtcgcgcg gcgtaaggtg
     2461 gcatacgaac cgtacttcag ctcgcgcccc ggttgggccg agcaggaccc cgagctgtgg
     2521 tggcgatcgc tcgtcgcggc ctctagcggg ctcgtcgccg aactacctgg cggcgccgac
     2581 cggatcgcgg ccctggcggt gacgacccag cgcgacacga tcgtctgcct cgacggggac
     2641 ctgcgccccc tgcgcccggc catcctgtgg ctcgacgcgc gcagggcgcc ggcgccgtac
     2701 catccatcgg ccctggtggc cctcggcgtg tcgctcgtcg gcatgcgcga ggctctccaa
     2761 aagacgcagg aagccggcaa gtgcaactgg atcatggcca acgagccggg gctctgggaa
     2821 cggacgcggg cctgcgtgct ggtctccggt ttcctgaacg cgaggctctc gggcgagctg
     2881 gcggactcga ccgcgtcgca gatcggccac ctgcccttcg attaccggcg tcaggcctgg
     2941 gccgccccgg gtagccgcaa cgcgaggatg ttcccgatag cgccggagaa gctgccgcgc
     3001 ctcgcgcccg ccggtacgga actcgggcgc ctgtcggccc gggcggccgc cgagctcggg
     3061 ctgcccgccg gcctgccggt gatagcggcc ggcagcgaca agggctgcga gacgataggc
     3121 tcgggcgtgg tcgacgagac ggccgcgagc ctgtccttcg gcacgacggc gaccgtgcag
     3181 acgaccagcc agcgctaccg cgaaccgata gccttcatgc cggcg
//
LOCUS       DAOOLN010000068         3193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_36780_length_3193_cov_11.477374, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000068 DAOOLN010000000
VERSION     DAOOLN010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36780_length_3193_cov_11.477374"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PLW80_01385"
     CDS             <1..369
                     /locus_tag="PLW80_01385"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 family protein"
                     /protein_id="HPB65184.1"
                     /translation="VGMVAIGVGLGLAFGFASILFALPLSWVLGVPYALALVLTAFAP
                     EDFAAIAWDSAGVTTGPITVPLVIATGLGIGGHAGVASSFGVVAAASVFPVVAVLGSG
                     LWRTVQARSALAAMSQGGRS"
     gene            366..968
                     /locus_tag="PLW80_01390"
     CDS             366..968
                     /locus_tag="PLW80_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65185.1"
                     /translation="MIRSALSKITCAVDSRLSAIIDEALSDMAIPEAFIQRAKQVSFI
                     ERSGPFGLAARAALSESRAELYRFYVPAGLESAAARRVAEAADLYLPGRGTLFAEDVE
                     VLSAEAPAYDESAISSACEEGSCSRPLEHYSVLCCIVQRGMGEDLARTVLEMGLCVPI
                     VSFGEGMGLRNKLGLLRITIPVDKEILYFVVSRSVSICRF"
     gene            complement(997..1743)
                     /gene="istB"
                     /locus_tag="PLW80_01395"
     CDS             complement(997..1743)
                     /gene="istB"
                     /locus_tag="PLW80_01395"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HPB65186.1"
                     /translation="MDDLGLGFMAASVESFLSDQSHKDTTLAESIGALLDIEYSARKE
                     RTARTRLKLSGMPQSKRLDDFDLSWLKGGLTKERFEELRSLSFIDRKENVLLLGPSGV
                     GKTHLMLALGQKACLSGYTAYYMTCRDAIESLLHAREQNRLKRRLAWLRKPHVLLLDE
                     VGYEPLTPEQAHVFFQLVNARYETGSVIMTSNKAFGKWAELMSDEAVATAMLDRLLHH
                     AHVVSLKADSYRMKDRLRVGSATSGGYSFE"
     gene            complement(1782..3029)
                     /gene="istA"
                     /locus_tag="PLW80_01400"
     CDS             complement(1782..3029)
                     /gene="istA"
                     /locus_tag="PLW80_01400"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HPB65187.1"
                     /translation="MLEARLLQAQGHTQLEIAELLGVCERTVRNYMKQMPRARKRPKR
                     PSKLDPFKRVILDQLEKNPSYNGELIFERITGMGYAGKKTVMKEFVAKARRRIEATAV
                     QRFETEPGLQAQVDWKEFGKQVVDGKETKLYAFVMVLGYSRKPFVCFTTSMDQATLLA
                     CHVLAFVYFGGVPREILYDNMRTAFQPDQEGNWRPTKRLAALAVHYDFVPKRCRVRRP
                     ETKGKVERTIGYLDRNFWPRMDGEPLSLSLLNERVQEWLEAICDKRLDDLGESRADRF
                     GREAHSLKPLPAGRFDVRHVVPVVVSRESTIRYETNRYSVPPEHIGATALLYVHPLSR
                     LAELVVGERSVRSFPLARPGSRSSVSFDEDRAALAKRWNDDRTRLAIARVPRKRRPRT
                     LVEVAVRSPSSYEAFAEAVEARA"
BASE COUNT          507 a         1016 c         1022 g          648 t
ORIGIN      
        1 gtcggcatgg tcgcgatcgg cgtcggcctc ggcctggcct tcggcttcgc gtcgatcctg
       61 ttcgccctgc ccctatcgtg ggtgcttggc gtgccctacg ccctggccct cgtcctgacc
      121 gccttcgccc cggaagactt cgccgctata gcctgggact ccgccggcgt gacgacgggt
      181 ccgataacgg taccgctggt catcgccacc ggcctgggta ttggcggcca cgccggcgtc
      241 gcctcgagct tcggcgtcgt ggcggccgcg agcgtgttcc ccgtggtggc cgtgctcggc
      301 tccgggctgt ggcggaccgt gcaggcgcgc tccgcgctgg cggcgatgtc ccaggggggc
      361 cggtcatgat acgatccgcg ttgtccaaga tcacctgcgc cgtcgattcg cggctatcgg
      421 ccatcatcga cgaggcgctc tccgatatgg ccataccgga ggccttcatc caacgggcca
      481 agcaggtatc gttcatcgag cgctccggcc ccttcgggct ggcggccagg gcggcgctgt
      541 cggagtcgcg cgccgagctg taccgtttct acgtacccgc gggcctggag tcggcggctg
      601 ctcggcgtgt cgccgaggcg gccgacctgt acctgcccgg ccgcggtacg ctcttcgccg
      661 aggacgtcga ggtactgtcg gccgaggcgc cggcctacga cgagtcggcc atctcgtcgg
      721 cctgcgagga gggctcctgc tcgaggccgc tcgagcacta ttcggtgcta tgctgcatcg
      781 tacagcgagg catgggagag gatttggcgc gcaccgtgct cgagatgggg ctgtgcgtgc
      841 ccatcgtctc cttcggcgag ggcatgggcc tgcgtaacaa gctcggcctc ctgcgcatca
      901 cgataccggt ggacaaggag attctctatt tcgtcgtgtc aaggtcggtt tcaatttgcc
      961 ggttttagtc ggtttgattt tgccggttcc cgtcacctac tcgaacgaat aaccgcctga
     1021 tgtagccgat ccgacgcgta gtcgatcctt catcctgtac gagtcggcct tgaggctcac
     1081 gacgtgcgcg tggtggagca agcggtcgag catggccgtc gcgaccgctt cgtcggacat
     1141 gagctcggcc cattttccga aggccttgtt ggaggtcatg atgaccgatc cggtctcgta
     1201 ccgcgcgttc acgagttgga agaatacgtg cgcctgctcc ggtgtcagcg gctcatagcc
     1261 cacttcgtcg agtagcagga cgtgcggctt cctgagccat gctagccgac gtttcagccg
     1321 gttctgctca cgcgcgtgga gtagcgactc gatagcatcg cggcaggtca tgtaataggc
     1381 cgtgtacccc gagaggcacg ccttctggcc gagcgcaagc ataaggtgcg tcttgccgac
     1441 gccggagggg ccgaggagca ggacgttctc cttgcggtcg atgaacgaga ggctccgcag
     1501 ttcctcgaag cgttccttcg tgagcccgcc cttcagccag gacaggtcga agtcgtcgag
     1561 gcgcttggac tggggcatgc ccgagagctt gagccgggta cgcgccgtcc gctccttgcg
     1621 agccgagtac tcgatatcga gcagcgcccc gatggactcg gcgagcgtcg tatccttgtg
     1681 ggattggtcg gaaaggaacg actccacgga cgcggccatg aagccgagcc cgagatcatc
     1741 catgcggctc gcgagggcgg ccgttgatat cgacggcccg ctcatgctcg cgcctcaacc
     1801 gcttcggcga aggcttcgta cgacgagggc gaacgaacgg ctacctccac cagagtccta
     1861 ggacgccgct tccgtgggac gcgcgctatg gctaatcgcg ttctgtcatc gttccagcgc
     1921 ttggccagcg cggcgcggtc ctcgtcgaag gatacggatg atcgactgcc cggacgcgcc
     1981 agaggaaagg accgtacact ccgctcgccg accacgagct cggcaagtcg cgacagcgga
     2041 tggacataca ggagcgccgt agcgccgata tgctccggcg ggacgctgta gcggttcgtc
     2101 tcgtaccgta tcgtcgattc ccttgaaacc acgacgggga cgacgtgcct gacgtcgaac
     2161 cgccccgcgg gcaggggctt gagcgaatgg gcttcgcgcc cgaagcgatc ggcccggctc
     2221 tcgccgagat catcgagccg cttgtcgcag atggcctcga gccattcctg tacccgctcg
     2281 ttcaggagcg agaggcttag gggctctccg tccatccgag gccagaagtt gcggtccagg
     2341 tagccgatcg tgcgctcgac cttgcctttc gtctccggcc tgcgtacgcg acaacgcttg
     2401 ggcacgaagt cgtaatgcac ggccagggcc gcgagtcgct tagtgggccg ccagttaccc
     2461 tcctgatcgg gctggaaggc ggtccgcatg ttatcatata ggatctcgcg cggcacgccg
     2521 ccgaagtaga cgaacgccag tacgtggcac gccaggagcg tcgcctggtc catcgaagtc
     2581 gtgaagcaga cgaagggctt tcgcgagtag ccgaggacca tgacgaacgc gtagagcttt
     2641 gtctccttac cgtcgacgac ttgcttgccg aattctttcc agtcgacctg ggcctggagc
     2701 ccgggttcag tctcgaagcg ctgtaccgcc gtcgcctcta tgcgccgacg agccttcgcg
     2761 acgaactcct tcatgacggt tttcttgccg gcatagccca tgcccgtgat acgctcgaag
     2821 atgagttcgc cgttatacga cgggttcttt tctagctgat cgaggatgac ccgcttgaac
     2881 ggatccagct tgctcggccg cttcgggcgt ttcctcgccc ggggcatctg cttcatgtag
     2941 ttccggactg tgcgctcgca gacaccgagt agttctgcta tctcgagctg cgtgtgtccc
     3001 tgcgcttgca gtagtcgcgc ttcaagcata cgattctccc tgtccatggg gctctctccg
     3061 tagatggttg ttgtggtgac tccatcatac agaggctgag ccccattctt cttcccctac
     3121 gacgtagggt tatggacagg aaaccggcaa attctccccg accaattccg gcaaattatc
     3181 accgactgtg aca
//
LOCUS       DAOOLN010000069         3178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_37022_length_3178_cov_3.185399, whole genome shotgun sequence.
ACCESSION   DAOOLN010000069 DAOOLN010000000
VERSION     DAOOLN010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37022_length_3178_cov_3.185399"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PLW80_01405"
     CDS             complement(<1..337)
                     /locus_tag="PLW80_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB65188.1"
                     /translation="MDDDRTILIVDDSRAFALAAAEMVEGEGYDVHLAHSATDALEAA
                     ERVRPRVVFLDVVLPDGEGYEVCSALKRTFMHRPLQVVMMSAVYDAKRMDRLLATGAD
                     DFIKKPFEAL"
     gene            complement(365..1933)
                     /locus_tag="PLW80_01410"
     CDS             complement(365..1933)
                     /locus_tag="PLW80_01410"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HPB65189.1"
                     /translation="MSLPELGTFLIPPLVGSVIGLFTNWLAIKMLFRPLAEKRLLGLR
                     VPFTPGILPRERERMARSLGETVAVDLLDERTVSERLRSQAFKDAVRSAAYGLGKKAL
                     EAKPGDLVSGLDGRTGKALRDVALELLSGVASSGVFARSVSAGAKAAVAASNAVKLSD
                     VVPGSAVGALAEALTGPEGGARTAAFLAEALMDAFEMAADEGKAVSDIVGADRLVALA
                     EKAVLAAYPSVIEATVGMMADKKVAASMEKAAARVIKRALDRFNSVQRFFISLGQYDK
                     AIMENMPATIADLGDSMRGILSEPSTKAAVLAKAAAAARGFAARPLAGFGFMRLAESR
                     EAARLSLEEALRDALAPLEPRAIAGMISGMLDRASLGEILEALPGVAERIGPALARWL
                     AGLSSKRPPDGSAGGAIAAAFFAAFASSFGSEASGAPLSETVSIDDEALGAIAASAAD
                     GLSELAAAQSGEVIRSIDIRSLVVDKIDSLDMIEVERMILKVVDKELGAITMFGGILG
                     AVIGLFQSLLFMLR"
     gene            complement(1930..2316)
                     /locus_tag="PLW80_01415"
     CDS             complement(1930..2316)
                     /locus_tag="PLW80_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65190.1"
                     /translation="MIKTRIAASTALAIVAATTMALAACSVDKNPRVELNETPIISGG
                     LGWGVVSLSYARLMLEPSLDAADSGTCRRGDVGELLARTRVAKGRDGGVWYRARFDGG
                     TGWLHESALAVFRTENEARNAVEARP"
     gene            2511..2972
                     /locus_tag="PLW80_01420"
     CDS             2511..2972
                     /locus_tag="PLW80_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65191.1"
                     /translation="MALSSGMGSGTAPGAAVSAELGARIGGDRLAAMIRAGVVKQAAS
                     AYTDVWTRYRGFSGLSLAAGPSLSLLEAGRLELRLLAGGALARYDLSYSYFAFPYIEP
                     GASYCVAWLDDRFSLRLGLSLPVYFRADALSFGARAVAELRYASPAAEPAP"
     gene            2969..>3178
                     /locus_tag="PLW80_01425"
     CDS             2969..>3178
                     /locus_tag="PLW80_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65192.1"
                     /translation="MSRRSGRAILAALAALGSAVAAASMAACAMPEPPNRVALVYGVS
                     IYLESLPQTKRPNLSLTDDDAYAIGA"
BASE COUNT          533 a         1097 c          999 g          549 t
ORIGIN      
        1 cgagcgcctc gaacggcttc ttgatgaagt catccgcgcc agtcgccaaa agcctgtcca
       61 tgcgcttggc atcgtagact gcggacatca tcacgacctg gagcggtctg tgcatgaagg
      121 ttcgcttgag ggctgaacag acctcgtagc cctcgccgtc gggtagcacc acgtcgagga
      181 acacgacgcg aggacggacg cgctcggcag cctcgagcgc gtcggtggcc gaatgggcca
      241 ggtgcacgtc atagccctcg ccctcgacca tctcggccgc ggcgagcgcg aaggcgcgcg
      301 agtcgtcgac tatcaggatc gtcctgtcat catccacttt ttacctcacg gtcggggccc
      361 ctcgctatcg cagcatgaac aggagactct ggaaaaggcc gatgacggcc ccgagtatgc
      421 ctccaaacat ggtgatggcg ccgagttcct tatccactac tttcaggatc attcgttcta
      481 cttcaatcat atccaacgag tcgattttat caaccacgag ggaacgaata tctatggacc
      541 tgatcacctc gccgctctgg gccgccgcca gctcggaaag gccatcggca gccgaggccg
      601 cgatcgcgcc gagcgcctcg tcgtcgatcg acacggtctc cgacagcggc gcgccagacg
      661 cctcggagcc gaaggacgac gcgaaggccg cgaagaaggc ggccgctatg gctccgccag
      721 ccgagccgtc gggggggcgc ttcgaggata ggccggccag ccaacgggcc agggcgggtc
      781 ctatgcgctc ggccactccg ggcaaggcct cgagtatctc gccgaggctg gcccggtcga
      841 gcatcccgga gatcatgccg gctatggctc gcggctcgag cggagccagc gcgtcgcgga
      901 gcgcctcttc gagggacagc ctggcggcct cgcgcgactc ggctaaccgc atgaagccga
      961 aaccggccaa aggcctagcg gcgaaccccc tggctgcggc cgcggccttg gccagtacgg
     1021 ccgccttggt cgaaggttcc gacagtatcc cgcgcatcga atcgcccaag tcggcgatgg
     1081 tcgctggcat gttctccatg atggccttgt cgtactgccc aaggctgatg aagaagcgct
     1141 gcaccgagtt gaaacggtcc agggctcgct tgatgacgcg ggccgcggcc ttctccatcg
     1201 acgcggcgac cttcttgtcg gccatcatgc cgacagtcgc ctcgatcacc gacggatagg
     1261 cagcgagtac ggctttctcg gctagggcga caagcctgtc ggcaccgacg atatcggaga
     1321 cggccttgcc ctcgtcggcc gccatctcga aggcgtccat gagcgcctcg gccaggaagg
     1381 ctgcggtcct ggctccgccc tcgggtccag tcagggcctc ggctagagcc ccgacggccg
     1441 agcctggcac cacatcggac agcttgacgg cattcgacgc agctacggcg gccttggcgc
     1501 cggccgatac cgagcgggcg aagacgccgc tcgaggcgac gccggacagc aactcgagcg
     1561 ctacgtctcg cagggccttt ccggtcctgc cgtccagccc cgatacgagg tctcccggct
     1621 tggcctcgag ggccttcttg ccgagtccgt aggccgccga gcgtacggca tccttgaacg
     1681 cctggctacg cagccgttcc gagactgtgc gctcgtcgag gagatcgacc gcgacggtct
     1741 cgccgagcga gcgggccata cgctctcgct cacgtggaag tatgcccggc gtaaacggaa
     1801 cgcgcaggcc gaggagccgt ttttcagcca gcggcctgaa tagcatcttg atcgccagcc
     1861 aattggtgaa caggccgatg accgaaccta ccagcggcgg gatcaggaaa gtgcccagct
     1921 ccggaaggct cacggcctcg cctccaccgc gttcctggcc tcgttctcgg tcctgaacac
     1981 agccaacgcc gattcgtgca gccagccggt ccctccgtcg aagcgagcgc ggtaccagac
     2041 gccgccgtcg cggcctttgg ctactcgcgt cctggccaat agctcgccga cgtcgccgcg
     2101 tcggcaggtg cccgagtcag ccgcgtcgag cgaaggctcg agcatcagcc tggcgtagga
     2161 cagcgagacg acgccccagc cgaggccgcc ggatatgata ggcgtctcgt tgagctctac
     2221 gcgcggattc ttatcgaccg agcaggctgc aagcgccatc gtcgttgcgg cgacgatggc
     2281 caaggcggtg gaagcggcga tacgggtttt aatcatcgaa tccctttcta ttcggtacac
     2341 gaacctgagt cgatttgggt actggttgaa tttatccaaa tttcgaggca tgttaaagcc
     2401 catctatgcg cgttattcga ttaaccatac tagcttcgct cctggcctcg acggcggcct
     2461 tcgcggcgcc cgctccggcc gtatcgttct tagcctcagt agcacccggc gtggccctga
     2521 gttcaggcat gggctcgggc accgcccccg gcgccgcggt ctcggccgag ctaggcgctc
     2581 gtatcggcgg cgaccgatta gccgcgatga tccgggccgg cgtcgtcaag caggctgcct
     2641 cggcctatac cgacgtctgg acgcgctacc gaggcttctc cggactgagc ctagccgccg
     2701 gcccgagcct aagcctactc gaggccggcc gcctcgagct caggctgctg gccggcggcg
     2761 cgctggcccg ctacgacctg tcgtactcgt acttcgcctt cccgtatata gagcctggag
     2821 cctcgtactg cgtcgcctgg ctcgacgacc gcttctcgct caggctgggc ctgtccctgc
     2881 ccgtatactt ccgggccgac gccctatcct tcggagcccg ggccgtagcc gagcttcgct
     2941 acgcctcgcc ggccgcggag cccgcgcctt gagccgtcgc tcgggccgcg ccatcctagc
     3001 cgcgctagcc gcgctcggat cggcggtcgc cgcggcctcc atggccgcct gcgcgatgcc
     3061 ggagccgccg aaccgggtcg cgctggtcta cggcgtttcg atatatcttg aaagccttcc
     3121 tcagactaaa cgtcctaacc tgagcctgac cgacgacgac gcctacgcca taggcgcg
//
LOCUS       DAOOLN010000070         3172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_37111_length_3172_cov_2.669233, whole genome shotgun sequence.
ACCESSION   DAOOLN010000070 DAOOLN010000000
VERSION     DAOOLN010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37111_length_3172_cov_2.669233"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1822)
                     /locus_tag="PLW80_01430"
     CDS             complement(<1..1822)
                     /locus_tag="PLW80_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPB65193.1"
                     /translation="MTINTFSQVIMSPLLSRLSVLACAAALFFSLRKKDAEGRSGSFL
                     VIIAFLVVRDTFLAVVDLPGAPYLTDLLYFSLAAFMVMAPFERSRLMLIATMAVNAAA
                     AGLYVAYAYAGVLPSLPAAAFPLVPVADALFVAASGAVNASDRGDGSSRLMSESWPVV
                     FACLGAYAAAAFFLGYESAVFVRLVVPASHAWLLVVALIDLRDHDAETIKAVSYYEES
                     IDSLYNLTFRVGTVLKGSFTTEDVLASMNEVMIAETGADGGTIYLVDEFDDIIVAKAY
                     AGTFPPPFPLPESLPRKQSRIESYLRHAQFRLGETVLGGVAKTGKNVYSPDTANDPRF
                     AALRAEGANMGEDFLRTSSFMAVPLMVEDKIIGVSAVARVGSGDQFDEAAFDRFKLLA
                     NFGTLAVSNFFSFLAANERSAIERSAGIAAEIQSTIIPKKLPQFPSLAIGAFTSPARG
                     VYGDYYDVLRTREDRVVGVIGDVAGKGVQAALVMVMIRAILHLITNTNKDIATVLDWV
                     NRGITGKIDMDHYATLAIVALNVATGELEYANAAHQPLLVYRRSTDTIETIETKSVPI
                     GVERANEYRRKALRLYDGDIAVLYTDGIVEAMNEQGKQFGR"
     gene            2024..>3172
                     /locus_tag="PLW80_01435"
     CDS             2024..>3172
                     /locus_tag="PLW80_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65194.1"
                     /translation="MVYCCIYIDYLSQLERTGVFEVTAFLDELGSTAAEFGGVVGSRK
                     DPLVVAFPSGGLFDGIQAVEALRRTVESLTVREAALRGASLTVYEGESVDDAVAFAAA
                     IRYRDHSAYSKTLSPEAASRLAAYFPSGDDGWRQPLHVGLMADADATRLFDRPRLAAT
                     LAKAIGKSRHGPRLVHLDAGSSVRSIDAIAQEMELEPERALILCGARLRPIPFSPLIE
                     AIMARFRDARGDGALVGDDGLLSGDDALLSGDDALLSGDDAMAFRFVEASSFSAAAPG
                     SVAMGCAAFIGSWLDAFGASGGVVACDAPERFSPEARELIAARLESGRGAERYLSIAS
                     GGPVERWIGPWAARVPAGLADADDRPSALSLALGSTEGPTRAALAERFG"
BASE COUNT          546 a         1018 c         1083 g          525 t
ORIGIN      
        1 tgcggccgaa ctgcttgccc tgctcgttca tcgcctcgac gataccgtcg gtgtacagca
       61 cggcgatgtc gccgtcatag agcctgaggg ccttgcgcct gtattcgtta gcacgctcca
      121 cgcctatcgg cacgctcttc gtctcgatgg tctcgatcgt atcggtcgag cggcggtaca
      181 cgagcagcgg ctgatgggcc gcgttggcgt actcgagctc gcccgtagcc acgttgagcg
      241 ccacgatagc gagcgtcgcg tagtggtcca tgtcgatctt gccggtgatg ccgcggttga
      301 cccagtcgag cacggtagcg atgtccttgt tcgtgttcgt gatcaggtgg agtatcgcgc
      361 gtatcatcac catgacaagg gccgcctgga cgcccttacc ggccacgtcg cctatcacgc
      421 ctactaccct gtcctcgcgg gtacgtagca cgtcgtagta gtcgccgtac acgccgcggg
      481 ccggcgaggt gaacgcgccg atggcgagcg acgggaactg cggcagcttc ttcgggatga
      541 tggtgctctg gatctcggcc gcaatgccgg cagaacgctc gatcgcgcta cgctcgttgg
      601 cggccaggaa cgagaagaag ttactgacgg ccagggtgcc gaaattggcc agcagcttga
      661 agcggtcgaa ggccgcctcg tcgaactggt cgcccgaacc cacccgggcg acggccgaca
      721 cgccgatgat cttgtcctcc accatcagcg gcacggccat gaacgaggag gtcctgagga
      781 aatcctcgcc catgttcgcg ccctcggccc gtaacgccgc gaagcgcggg tcgttcgccg
      841 tgtccggcga gtagacgttc ttgccggtct tggccacgcc gccgagcacg gtttcgccga
      901 gcctgaactg ggcgtgccgc aggtacgact cgatccggct ctgcttgcgc ggcaggctct
      961 cgggtagcgg gaagggcggc gggaaggtgc cggcgtaggc cttggctacg atgatatcgt
     1021 cgaattcgtc cacgaggtag atcgtgccgc catcggcccc ggtctcggcg atcatgacct
     1081 cgttcatgct ggcgagcacg tcctccgtcg tgaagctgcc tttgagcacg gtaccgacgc
     1141 ggaaggtcag gttatacagc gagtcgatgg actcctcgta gtaggagacg gccttgatcg
     1201 tctcggcgtc gtgatcgcgc aggtcgatga gggcgacgac gagcagccag gcgtgcgacg
     1261 cgggcaccac gagccgaacg aacaccgccg actcgtagcc gaggaagaac gcggcggcgg
     1321 cgtacgcgcc gagacaggcg aagacgaccg gccagctctc ggacatcagc cgactggatc
     1381 cgtctccacg gtcggaggcg ttgacggcac ccgacgcggc gacgaacaga gcgtcggcca
     1441 cgggcaccag agggaaggcg gcggccggta acgacggcag gacgccggca taggcgtagg
     1501 cgacgtagag tccggccgcg gccgcgttga cggccatggt cgcgatcagc atgagcctcg
     1561 atcgctcgaa cggcgccatg accatgaacg ccgccaggct gaaatagagg aggtcggtca
     1621 ggtacggcgc gcccggtagg tcgacgacgg ccaggaaggt gtccctgacg acgaggaagg
     1681 ctatgatgac gaggaacgat ccggagcgcc cctcggcgtc cttcttgcgt agcgagaaga
     1741 acaacgcggc cgcgcacgcc agcacggata gccgggacag gagcggcgac atgataacct
     1801 ggctgaacgt attgatagtc ataggattcc cttgaggtta tgctcggacc gaaaggtcgc
     1861 gggaccgctc gtacccgcta gggcgcaagt gtagcatggc aaaaaaaata tcgtcaagcg
     1921 aaaaggaaag ctggaagccc ggcagcctcg aatcccgctt cacgatcggc ccggcatgga
     1981 ctatactcgc ctgagagaaa aacgcacggg ggacgttcgc ctcatggtgt actgctgcat
     2041 ctacatagat tacctgtctc agctcgagcg taccggcgtg ttcgaggtca cggccttcct
     2101 ggacgaactc gggtcgacgg cggccgaatt cggcggcgtg gtcggctcca ggaaggatcc
     2161 gctcgtcgtc gccttcccct ccggcggtct cttcgacggt atccaggcag tcgaggccct
     2221 gcggaggacg gtcgagtcgc tgacggtccg cgaagccgcg ctacgcgggg cgagcctgac
     2281 tgtctacgag ggcgaatcgg tcgacgacgc cgtggccttc gcggcggcga ttcgctatcg
     2341 cgaccattcg gcgtactcaa agacgctcag ccccgaggcg gcgagccgcc tggcggccta
     2401 tttcccgagc ggcgacgatg gctggcggca accgctccac gtcggcctga tggccgacgc
     2461 cgacgcgacc cgtctgttcg accggcccag gctcgctgct acgctggcca aggccatcgg
     2521 caagtcgagg cacggaccga ggctcgtcca cctcgacgcc ggctcgagcg tccggtctat
     2581 cgacgcgata gctcaggaga tggagctgga gccggagcgc gcgctgatac tctgcggcgc
     2641 caggctacgc cccataccgt tctcgccatt gatcgaggcg atcatggcgc gcttccgaga
     2701 cgcgcgcggc gacggcgcgt tagtaggcga cgacgggtta ttaagcggcg acgacgcgtt
     2761 attaagcggc gacgacgcgt tattaagcgg cgacgacgcc atggccttcc gcttcgtcga
     2821 ggcgtcctcg ttctcggccg cagctcccgg ttcggtagcc atgggatgcg ccgcgttcat
     2881 aggctcctgg ctcgacgcct tcggtgcctc gggcggagtc gtcgcctgcg acgctcccga
     2941 acgtttctcg cccgaggccc gcgagctcat cgcggcgagg ctggagtccg gccgcggcgc
     3001 cgagcgctac ctgtccatag catctggcgg accggtcgag cgctggatag gcccctgggc
     3061 ggccagggtc ccggcgggcc tcgccgacgc cgacgacagg ccgagcgccc tctccctggc
     3121 gctcggctcg accgaggggc cgactcgcgc ggcgctcgcg gagcgcttcg gc
//
LOCUS       DAOOLN010000071         3162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_37275_length_3162_cov_2.598326, whole genome shotgun sequence.
ACCESSION   DAOOLN010000071 DAOOLN010000000
VERSION     DAOOLN010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37275_length_3162_cov_2.598326"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..725
                     /locus_tag="PLW80_01440"
     CDS             261..725
                     /locus_tag="PLW80_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPB65195.1"
                     /translation="MSPERSYKIVELTRMFGLTARTLRYYEELGLLESEDRDATEHRR
                     YSERNVVRLKRVQQLKDYGLTLSEIRELFDLAKRDRTGVSVRESLAAKYRARLEDAKR
                     RRESLDAYIEDLSWHVEQLERVSDFFDCPGASCAACRWAERCDVRALIAKDA"
     gene            728..2185
                     /locus_tag="PLW80_01445"
     CDS             728..2185
                     /locus_tag="PLW80_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPB65196.1"
                     /translation="MYCGIDVGTSSVKCLITDASGSRLGLGKAPVGLHLGDRGFEADP
                     MEWLRATAIAYAEAATGIGEATLAAGLAAVAVSGNGPTLVTVGGDGLPIGRAASWLER
                     GAVAEAERVSALAGRRVDPSFYLPKALRAIESPQGDDVAMFFSGPEYLAFALGAAPVS
                     YLADDYYDAFVWDRAVAARLGLADSLFPRYIRPATVIGSVSRQAAEAVGLPAGLPIVA
                     GFPDFLAALVGSGAVETGLACDRSGSSEALNVCADRPFPDRAIFSLPHAVSGLWNLSG
                     GLSTSGTAVEWFARASGYSTGGVSGYDGNEALFLDAERSNGDVPIFLPYLSGERAPLW
                     EPGLRGAFIGLSLDHGRKEMARAVVESIAFGLRLAADRIRGGGFDIRLARCSGGAARD
                     ASLCAIKADVLGLPVEVPASPECEAMGDACACAVALGERSSLAEASASMVAVGARYEP
                     DPRRAAAYDERYERWERALDAVLSMSAERAPKEKR"
     gene            2187..3140
                     /locus_tag="PLW80_01450"
     CDS             2187..3140
                     /locus_tag="PLW80_01450"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HPB65197.1"
                     /translation="MIVLPPSMMQSLILFLLICVGFAAVKTRTLSPDNVRALSRFLVD
                     YTLPAVIIISMQRPFSPALRDQALRILGLSLLFYAASFPLAYLATAAYRKSGRAELGV
                     HRFAMCFSNVAFMGFPVAESLLGKESLFILSIYNIPFQVLAFSVGIVMISGRRSGAGA
                     PGTRHPSLGSLRSLVNPSIVSAVAGFALFLGSVRIPEPLYSAMDLLGGMTTPLAMVVI
                     GATLANTRIGGVLSNPRLWLTTAYRLALLPALAFAAAKAAGLTGLELSIPVLVAAMPV
                     AANSTILAGVYGGDDVTASALVFASTLLSLFTIPALGYLIA"
BASE COUNT          465 a         1143 c         1006 g          548 t
ORIGIN      
        1 aacacggcga ccgacagcgg atcggctacc ttcgtaggct tcgcgcacga ggctccgagc
       61 atcaggcccg cggcgcaggc gatggcggca aacggacgaa cgtatcggtg cataatcgaa
      121 ctcctgtctt ggcgcctcca tcattccggc tcgaggcgcg ctattcacga aactcggcca
      181 tgagtatact tatcgcggtg ctagtcgcgg agtagccgca ggcttccgtt cttgaaagct
      241 tacgttaaag gtcaacctga atgagccccg agcgatcgta taaaatagta gaactaacgc
      301 gcatgttcgg cctgacggcc aggacgttgc gctactacga ggagctcggc ctcctcgagt
      361 ccgaggaccg ggacgcgacc gaacatcgcc gctactcgga acgtaacgtc gtgcgcctga
      421 agcgcgtgca gcagctcaag gactacggcc tgacgctgtc ggagatacgc gagctgttcg
      481 acctggccaa gcgcgatcgc accggcgtat ccgtcaggga gagcctcgcg gccaagtacc
      541 gcgcaaggct cgaggacgcc aagcgacgcc gcgagtcgct cgacgcctat atcgaggacc
      601 tgtcctggca cgtcgagcag ctcgagcgcg tgagcgactt cttcgattgc cccggcgcgt
      661 cctgcgccgc ctgccgctgg gccgagcgct gcgacgtacg cgccctgata gcgaaggacg
      721 cctaaccgtg tactgcggca tcgacgtcgg cacctcatcg gtcaagtgcc tcattaccga
      781 cgcgagcggc tccaggctag gcctcggtaa ggccccggtc ggcctgcact taggcgacag
      841 gggcttcgaa gccgacccga tggagtggtt gcgcgcgacc gccatcgcct acgccgaggc
      901 cgcgacaggc attggcgaag ccacgctcgc ggcggggctc gccgcggtag ccgtatcggg
      961 taatggcccg accctggtga ccgtcggcgg agacggactg ccgatcgggc gcgccgcctc
     1021 gtggctggag cgaggcgccg tcgccgaggc cgagcgagtc tcggccctgg ccggccgccg
     1081 cgtcgacccg tcgttctacc tgcccaaggc cctgcgcgcc atcgaatcgc cccaaggcga
     1141 cgacgtagcc atgttcttct ccggcccgga atacctggcc ttcgcgctcg gcgccgcccc
     1201 tgtatcctat ctcgcggacg actactatga cgccttcgtc tgggacaggg ccgtcgcggc
     1261 tcggctcggg ctagccgact cgctctttcc ccgctacata cgccccgcga ccgtcatcgg
     1321 ctcggtcagc cggcaggccg ccgaggcggt cggcctaccc gccggcctgc ctatcgtcgc
     1381 cgggttccct gacttcctcg ccgctctcgt tggctccgga gcggtcgaga cgggcctcgc
     1441 ctgcgaccgt tccggctcga gcgaggcgct caacgtctgc gccgatcgcc cgttcccgga
     1501 ccgcgccatc ttcagcctgc cccacgcggt gagcggcctc tggaacctat cgggcggcct
     1561 gtcgacgagc ggcacggcag tcgagtggtt cgcccgcgcc tccggctact cgacgggagg
     1621 agtatccggc tacgatggca acgaagcact gttcctggac gcggagcgct ccaacgggga
     1681 cgtgccgata ttcctcccat atctatccgg cgagcgagcg ccactctggg agccgggtct
     1741 gcgaggcgcc ttcatcggcc tgtccctgga ccacggcagg aaggagatgg ctcgcgccgt
     1801 ggtcgagtcg atcgccttcg gcctgcgcct cgcggccgat cgcatacgcg gcggcggctt
     1861 cgacatccgc ttggcgcgct gctcaggcgg ggccgcccgc gacgcctccc tctgcgccat
     1921 caaggccgac gtcctgggcc tgcccgtcga agtgccggcg tcgccggagt gcgaggccat
     1981 gggcgacgcc tgcgcctgcg cggtcgccct cggcgaacga tccagcctgg ccgaggcgtc
     2041 ggcctccatg gtcgccgtcg gggcccgcta cgagccggat ccgcgacgcg cagccgccta
     2101 cgacgaacgc tacgaacgtt gggaacgggc cctcgacgcc gtactctcga tgagcgccga
     2161 acgggcgccg aaggagaagc gctagcatga tcgtcctgcc tccctcgatg atgcagagcc
     2221 tcatcctatt cctgctcatc tgcgtcggct tcgctgccgt caagacccgt accctgtctc
     2281 ccgacaacgt gcgcgccctg tcgcgcttcc tcgtggacta caccctgccg gccgtcatca
     2341 tcatatccat gcagcggccc ttcagccccg cgctgcgcga ccaggccctg cgcatactgg
     2401 gcctctcgct gttgttctac gcggcgtcct tcccgctcgc ctaccttgca accgcggcgt
     2461 accgcaagtc cgggcgagcg gagctcgggg tgcaccgctt cgcgatgtgc ttctcgaacg
     2521 tcgccttcat gggcttcccg gtggcggagt cgctcctagg caaggaaagc ctgttcatcc
     2581 tctcgatcta caacataccg ttccaggtac tcgccttctc ggtcggcatc gtgatgatat
     2641 cggggcgacg ctcgggcgcc ggtgcgccgg gaacgcgcca ccccagcctc ggctcgctcc
     2701 gttcactcgt caacccgtcg atcgtgtccg cggtcgcggg ctttgcgctg ttcctcggtt
     2761 cggtacgcat acccgagccg ctgtactcgg cgatggacct gctcggcggc atgaccacac
     2821 cgctggcgat ggtcgtcatc ggcgcgacgt tggccaacac gcgcatcggc ggagtcctgt
     2881 cgaacccgcg gctatggctg acgacggcct atcgcctcgc cctcctgccc gccctggcgt
     2941 tcgccgcggc caaggccgct ggacttacgg gcctggagct gtcgattccg gtgctcgtgg
     3001 cggctatgcc cgtggcggcc aatagcacca tactcgccgg cgtctacggc ggcgacgacg
     3061 tgaccgcctc ggccctggtc ttcgcctcga cgctactgtc gctgttcacc atccccgcgc
     3121 tcggctacct gatagcctga cccgagcctc ggcgccgcgg tt
//
LOCUS       DAOOLN010000072         3153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_37402_length_3153_cov_2.595868, whole genome shotgun sequence.
ACCESSION   DAOOLN010000072 DAOOLN010000000
VERSION     DAOOLN010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37402_length_3153_cov_2.595868"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PLW80_01455"
     CDS             <1..576
                     /locus_tag="PLW80_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65198.1"
                     /translation="SISDIVLRSRASTRRLFGLEPTGGLPAAASPATFQASADLAASA
                     TQTQAIDTVEPEASAMFVDRPSVSDISGTWRGDKGLETVRIFPNGAGLAVLSGGGTLK
                     LRVTIRGGEVVIAQDQANDAALYKASSVSFAMAKAIAERARPMRWVFRLDATRDLLSG
                     MKESVAVSYSGDELLIDNDYVREASWTRISR"
     gene            complement(537..1022)
                     /locus_tag="PLW80_01460"
     CDS             complement(537..1022)
                     /locus_tag="PLW80_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65199.1"
                     /translation="MKRIVILACWIAVASYCAMAVVWGPSGVIVTKRAFDTAATMRRN
                     IDDLARRNERYAAELEGLRNASEPTALEARSIGYLAADEVAVRLSLEPEPAVPAEAGR
                     LLEYTGASALAEARIKDIAAMAGLVVLLAGLALRAARSARSAQGRAGQREILVQEASR
                     T"
     gene            1198..>3153
                     /locus_tag="PLW80_01465"
     CDS             1198..>3153
                     /locus_tag="PLW80_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65200.1"
                     /translation="MQNKTQQGKPDPIPTPEHDLERYGVWVKAEPKDLTDDTLVADGS
                     VASTSAEGSDAVLLAEEEELLDSFDLPNLDTAVETPAETPLEGEAESIDFDDFESLAP
                     LDDEDGISEADVPDTGDELDDGVVESTVIDMSLDDLDFDEVKTEAPAAEPPAERRSAS
                     VDAFETTEISIEDFGLDDDGPTGPALDDLERFAASPASASPALADPAEADEEFESLDI
                     DLQFDDTIPSAESEEATDTFSSEDDILGSSSEFESIDLDSIGLDEPKPKPAPVAVREA
                     PIASEPTDDSEGISLDSFIDSDDDASIGIIPDLEIESVSIGSGESGFDDIQAVSDDLA
                     SSAPSSDLLRQIASELSSIKQELVSLRDQLSSIKAAGASVGEAASAEGVEDELADASG
                     GFFDDEDDDTIALTGDELDNILNTADFTEETVSEEPEDEAPELSIPDDVELLPEDGVY
                     AEASEPGIETIELAEEATSFADADLSGEGIEAVEPIDGVTPIIPGPEDTSFLDDDTEA
                     RDFQELEGMPIEDVPLVEPSETDLDIDLAPAFEGDDEDLPVIEAADDESELVLDIDAG
                     DEPVVSTVDSFDEPIDEVEDAMDLEEVIEPEELGGLELHAEKSTEPVEELLPSDDVLE
                     ELTLDDFGDDSVDELVVEAPLETPQASD"
BASE COUNT          644 a         1015 c          962 g          532 t
ORIGIN      
        1 tccatcagcg acatcgtgct acgctctcgc gcttccacga ggcggctatt cggcctcgaa
       61 ccgacgggcg gcctaccagc ggccgcgagc cccgcgacct tccaggcttc ggccgaccta
      121 gcggcatccg ccacgcagac acaggccatc gacacggtcg agcccgaggc gagcgccatg
      181 ttcgtcgacc ggccgagcgt ctcggacatc agcggcacct ggaggggcga caagggcctc
      241 gaaacggtac gtatcttccc gaatggcgct ggcctggccg tactgagcgg aggcggcacc
      301 ctaaaactgc gcgtgacgat acgcggagga gaggtcgtca tcgcccagga ccaagccaat
      361 gacgccgcgc tgtacaaggc ctcctcggtc agcttcgcga tggccaaggc tatcgccgag
      421 cgcgccaggc ccatgcgatg ggtcttccgc ctcgacgcga cccgagacct cctgtccggc
      481 atgaaggaat cggtagccgt cagctactcc ggcgacgagc tacttatcga caacgattac
      541 gtccgggagg cttcctggac caggatctcg cgctgaccgg cgcgcccctg cgccgatcga
      601 gccgatcgag cggccctcag cgccaggccg gccaacagga cgaccaggcc ggccatcgcg
      661 gcgatatcct tgatccgcgc ctccgcgagc gccgaggcgc ctgtatactc gagtagtctg
      721 ccggcttcag ccggtactgc cggctcgggt tcgagcgata agcgaacggc tacctcgtca
      781 gccgccaggt agccaatcga cctggcctcg agcgcggtag gctcggaggc gttacgcagg
      841 ccttcgagct cggctgcgta gcgctcgttg cgcctggcca gatcgtctat attacggcgc
      901 atcgtcgcgg ccgtatcgaa ggcccgcttg gtcacgatga cgccgctcgg accccatacg
      961 acggccatgg cgcagtagga cgcgacggct atccaacagg caagtatcac tattcgtttc
     1021 atcactctat ttacggaacg agcgccttct ttctttacca gcgtcatccg gaattccgat
     1081 acggttgaca ataggaagaa gcgcgattag agtaatttca gtcgtatgct ctatgtattt
     1141 atccggtttc tgccgatatt tggatgagat caatccagtc gatggagtgc gcgcgccatg
     1201 cagaataaga cccagcaagg gaagccagat cccattccta cccccgagca cgatcttgaa
     1261 cgatacggcg tctgggtcaa ggcggagccg aaagacctga ccgacgacac gctcgttgcc
     1321 gatggatcgg tagcctcgac ttcggccgaa ggctcggatg cggtcctgct cgccgaggaa
     1381 gaggaactgc tcgattcgtt cgacctaccg aatctggata cggccgtcga gaccccggcc
     1441 gagacacctc tcgaaggtga ggccgagagc atcgacttcg acgatttcga atccctcgcc
     1501 ccgctcgacg acgaggacgg catctccgag gcggacgtcc ccgatacggg cgacgagctc
     1561 gacgacgggg tcgtggagag cacggtcata gacatgtccc ttgacgatct cgatttcgac
     1621 gaggtaaaga cggaggcgcc agcggcggaa cctccggccg agcgacgatc ggcctcggtc
     1681 gatgcattcg agaccactga gatcagtatc gaggacttcg gcctcgatga cgacggcccg
     1741 accgggcccg cgctcgacga cctagagcgc ttcgcggcca gcccagcttc ggccagccca
     1801 gctttagccg atccggccga agctgacgag gagttcgaat ccctcgatat cgaccttcag
     1861 ttcgacgaca ccatcccatc ggcggaatcg gaggaggcga ccgatacctt ctcctccgaa
     1921 gacgacatcc tgggctcgag ctcggaattc gaatcgatag atctcgattc cataggcctc
     1981 gacgaaccca agcccaagcc cgctcccgta gccgttcgcg aggcaccgat cgcgagcgag
     2041 ccgaccgacg attccgaggg catatcgctc gactccttca tagattccga cgacgacgcg
     2101 agcatcggga tcatccccga cctcgagata gagagcgtat ccatcggttc cggcgaatct
     2161 ggcttcgacg acatccaggc cgtatcggac gacctcgcct cctcggctcc gtcctccgac
     2221 ctgctcaggc agatcgctag cgaactgtca tcgattaagc aggagctcgt gagccttcgc
     2281 gaccagctga gcagcatcaa agcggctgga gcctccgtcg gtgaagcggc ctccgcggaa
     2341 ggcgtcgagg atgagctagc cgatgcatcc ggtggcttct tcgacgacga ggatgacgac
     2401 acgatagcct tgaccggcga cgagcttgac aacatcctca atacggccga cttcaccgag
     2461 gagaccgttt cggaggaacc cgaggatgag gctcccgaac tgtcgatacc cgacgacgtc
     2521 gagctattgc ctgaggacgg cgtctacgcc gaggcctcgg agcccgggat cgaaaccatc
     2581 gagctagcgg aggaagctac gagcttcgcg gacgctgacc tttcgggcga ggggatcgaa
     2641 gccgtcgagc ccatcgatgg agttacgccg atcatccccg ggcccgagga cacgagcttc
     2701 ctggacgacg ataccgaggc tcgggatttc caggagctcg agggcatgcc catagaggac
     2761 gttcctctcg tcgaaccgag cgagactgat ctcgatatcg acctcgctcc cgccttcgaa
     2821 ggggacgacg aggacctgcc ggtcatagag gccgccgacg acgaatcgga gctggtactc
     2881 gacatagacg ctggagacga acctgtcgtc tcgacggtgg actcgttcga cgaacccatc
     2941 gacgaggtag aggacgcaat ggacctcgag gaggtcatcg agcctgaaga gctaggcggg
     3001 ctcgagctcc atgccgagaa gtcgaccgag ccggtcgagg agctgctacc aagcgatgac
     3061 gtcctcgagg aactcacgct cgatgatttc ggcgacgatt ccgtcgatga gctcgtcgtg
     3121 gaggctccgc tcgagacgcc ccaggcatcc gac
//
LOCUS       DAOOLN010000073         3127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_37794_length_3127_cov_3.725911, whole genome shotgun sequence.
ACCESSION   DAOOLN010000073 DAOOLN010000000
VERSION     DAOOLN010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37794_length_3127_cov_3.725911"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..897)
                     /locus_tag="PLW80_01470"
     CDS             complement(28..897)
                     /locus_tag="PLW80_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65201.1"
                     /translation="MANAIARETRKAEDDPRKRVEAIVRAMALAFDSPLTGLELGARD
                     EYEALLAPDGGYNRATIRRVASVLNAYLALFGQDGVARAGARAASRVCEENDELLRYL
                     ASLEPPDRVAERKAGFVPANLGASFVRKGLGLPLFGRFRPSAPPTAPGLILAYRSAER
                     DGTARLYVTRNGPGAAITYAGLGAELSSAARAMECAIVPADVYEPEAGCDAHDFGRYV
                     VVMARRPSRPRRRPVTTLWDPAEYGFLYYIPDDGLAADRLARFLRDLLSGMFGVPHRE
                     AERLAALFSERSP"
     gene            complement(902..2026)
                     /locus_tag="PLW80_01475"
     CDS             complement(902..2026)
                     /locus_tag="PLW80_01475"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB65202.1"
                     /translation="MTAYNQSMLVNGTDILEPLGICYESLDGDGRIVPANERFWAMAD
                     SPMPSGPVSLDEGFAELVGLERELDALMHEGSGQVRLSGFRRGERYLNLTILPGVAVL
                     QDATGEMLAKLTVVQSKNEMAILDEALHSKNAELLEAYGRLDELMNTIRTHNHDLELE
                     VRKRTSELLRSRLSVITTLARVAEFRDTDTGRHTFRIGQASVMIGRELGLPEDRCEQL
                     YYASLLHDIGKIGIPDSILLKPGRLDESETRVMQGHTRIGAEILDRKDHSLFDTAKRV
                     ALYHHERWDGSGYPEGLAGEAIPLVGRICAVADVFDALLSKRPYKEPWPLDEALGEIA
                     ARGGTQFDPTIVAAFMRVADAIASLHDDDETGELLPPELD"
     gene            complement(2094..>3127)
                     /locus_tag="PLW80_01480"
     CDS             complement(2094..>3127)
                     /locus_tag="PLW80_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HPB65203.1"
                     /translation="LRELRKGSVIELPDDDEGRAWLRLGGARVAELSGLRADGDSLSA
                     DFAGRPTDAGASKGAADPMAALAAELKAGLAAVSSGVSEAVSSMARRIDELKGGQEDL
                     SDRVLFGQADSGGRSPGKPFSSLAGVPAEPFALFLSGERPQVCALVLSFLDDALAARL
                     LSLLSEAVRPEVTRRIATMDWVTPQALSETERILGKKLSAIGRSGFEADGIKKVVGIL
                     NFSPRETERSVITALDAIDPELSESIKRSMFVFEDIIILDDESIAAVLERADEKDFVV
                     AMKPMKDDERRRLLERLPAGSRERVGEAYDALGRVRMSDCDAAGFRVVEIVRSLEREG
                     RIGIVRQGD"
BASE COUNT          485 a         1066 c         1032 g          544 t
ORIGIN      
        1 aacgcgccct gaaccgtagc gtcaccttca aggcgatctt tcgctgaaca gggccgctag
       61 cctttccgcc tcgcggtgcg gtaccccgaa catgccgctg agcagatccc gtaggaaacg
      121 ggcgagcctg tcggccgcga gtccgtcgtc gggtatgtag tataggaagc cgtattcggc
      181 cggatcccag agagtcgtta ccggtcgccg tctgggccga gacgggcggc gggccatgac
      241 gacgacgtaa cggccgaagt cgtgggcatc gcaccccgct tccggctcgt atacgtcggc
      301 cggtacgatg gcgcattcca tcgcgcgcgc cgcgctcgac agctcggctc ccaatccagc
      361 atacgtgatc gcggcacccg gtccgttgcg cgtcacgtat aggcgagccg tgccatcccg
      421 ctcggccgaa cgatacgcca ggatcagccc cggggccgtg ggcggcgcgc tcggccggaa
      481 tcggccgaag agcggtaggc ccaagccctt ccgaacgaaa gacgctccca gattggccgg
      541 tacgaagccc gctttgcgct cggcgacccg gtccggcggt tcgagcgatg ccaggtagcg
      601 cagtagctcg tcgttctcct cgcatacccg gctcgcggcc ctggctccgg ccctggcgac
      661 gccgtcctgg ccgaataacg cgagataggc gttgagtacc gacgcgactc tgcgtatcgt
      721 ggcgcggttg tagccgccgt ccggggcgag caaggcctcg tattcgtctc gagcgccgag
      781 ttcgaggccg gtgagcgggc tgtcgaaggc cagggccatg gcgcgtacga tcgcctcgac
      841 gcgttttcta ggatcgtcct cagccttgcg cgtctcacgg gcgatggcgt tcgccatggc
      901 gtcaatccag ttccggcggc aatagctcgc ccgtttcgtc gtcgtcatgg aggctcgcga
      961 tggcgtcggc tacgcgcatg aacgccgcga cgatggtcgg gtcaaactgc gtaccgcctc
     1021 gcgccgctat ctccccgagc gcctcgtcga gcggccacgg ctccttgtac gggcgcttgg
     1081 acaggagcgc gtcgaagacg tcggccacag cgcagatacg cccgacgagc ggtatggcct
     1141 cgccggccag cccctccgga tagcccgaac cgtcccaccg ctcgtggtga tagagcgcga
     1201 ctcgtttggc cgtgtcgaag aggctgtgat ccttacgatc cagtatctcc gcgcctatgc
     1261 gcgtatggcc ctgcatgacg cgcgtctcgc tctcgtccag ccggcccggc ttgagcagga
     1321 tggagtccgg tatgcctatc ttgcctatgt cgtggagcag gctcgcgtag tagagctgct
     1381 cgcagcggtc ttccggcagc ccgagctccc ggccaatcat gacgctggcc tggcctatcc
     1441 tgaaggtgtg cctgcccgta tcggtgtccc tgaattccgc cacgcgggcg agcgtcgtta
     1501 tgaccgacag cctcgagcgc aacagctccg aggttcgctt cctgacctcc agctccagat
     1561 cgtggttgtg ggtcctgatc gtgttcatca gctcgtccag ccgaccgtag gcctcgagca
     1621 gctcggcgtt tttcgaatgc agggcctcgt cgagtatggc catctcgttc ttggactgaa
     1681 cgacggtgag cttggcgagc atttcgcccg tcgcgtcctg caacacggcc acaccgggta
     1741 gtatggtcag gttcaggtag cgctcgccgc gcctgaagcc gctcagcctg acttgtccgc
     1801 tcccctcatg catcagggcg tccagttcgc gctcgagtcc gaccagttcg gcgaagccct
     1861 cgtccaggct gactggcccg gagggcatgg gcgaatcggc catggcccag aagcgctcgt
     1921 tggctggaac gatccggcca tcaccgtcga ggctctcgta gcaaattccc aaaggctcca
     1981 gtatatctgt tccattgacg agcatacttt gattgtaggc cgtcatgccc ctacggtcaa
     2041 cgcgaggcgg atcggatcgg tacgaccgga tccgggccgc ctcgtgaacc gcgctagtct
     2101 ccctgcctga ctatgccgat gcgtccttcc cgctccaggg acctgacgat ctcgaccact
     2161 ctgaagccgg ctgcgtcgca gtcggacata cgcacgcggc cgagggcgtc gtaggcctcc
     2221 ccgacccgct cgcggctccc ggccggcagg cgctcgagaa ggcggcgccg ctcgtcgtcc
     2281 ttcatcggtt tcatggcgac gacgaagtcc ttctcgtcgg cccgctcgag cacggccgct
     2341 atcgactcgt cgtcgaggat gatgatgtcc tcgaagacga acatgctgcg cttgatcgac
     2401 tcggatagct cggggtcgat cgcgtccagg gcggtgatga ccgatcgctc cgtctcgcgc
     2461 ggcgagaagt tcaggatgcc gacgaccttc ttgatgccgt cggcctcgaa gccggagcgg
     2521 ccgatggccg acagcttctt gccgaggatg cgttccgtct ccgatagcgc ctgcggcgtc
     2581 acccagtcca tcgtcgcgat gcggcgcgtc acctcgggcc ggaccgcctc ggacagcagc
     2641 gacaggagcc gggccgccag ggcgtcgtcc aggaacgaca ggacgagcgc gcagacctgg
     2701 ggacgttctc cggacaggaa cagcgcgaag ggctcggccg gcacgccggc gagcgacgag
     2761 aaaggcttgc ccggcgagcg accgcccgag tcggcctggc cgaacagcac gcggtccgac
     2821 aggtcctcct ggccgccctt gagctcgtcg atgcggcggg ccatgctgga gacggcctcg
     2881 gaaacgcctg aggagacggc cgcgagaccg gccttgagct cggcggccag ggcggccatc
     2941 gggtcggccg cgcccttgga ggctccggcg tcggtcggcc gcccggcgaa gtcggccgac
     3001 agcgagtcgc cgtcggcgcg taggcccgac agctcggcta cgcgggctcc gccgaggcgc
     3061 agccaggccc gcccctcgtc atcgtcgggc agctcgatca cggagccctt gcgtaactcg
     3121 cggagga
//
LOCUS       DAOOLN010000074         3123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_37855_length_3123_cov_3.680248, whole genome shotgun sequence.
ACCESSION   DAOOLN010000074 DAOOLN010000000
VERSION     DAOOLN010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37855_length_3123_cov_3.680248"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PLW80_01485"
     CDS             complement(<1..205)
                     /locus_tag="PLW80_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HPB65204.1"
                     /translation="MSGNVFDRVLNAAGEALIIAELGTGHGGDELKAMELAAAAVEAG
                     ADCVKLQHVYADEIIHPETGLVPL"
     gene            303..548
                     /locus_tag="PLW80_01490"
     CDS             303..548
                     /locus_tag="PLW80_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65205.1"
                     /translation="MKHLFCDVCKKEVVDPIPTRTFFHIREFDLCEACHDDLEAAIKY
                     TVRGKKPFDFSWYDKMRIELVEDGVKKNKISSSKTQR"
     gene            800..1537
                     /locus_tag="PLW80_01495"
     CDS             800..1537
                     /locus_tag="PLW80_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPB65206.1"
                     /translation="MSGHSKWATIKHKKGAADAKRGQMFTKLIKEISIAARMGGGDPD
                     TNARLRTAVLKARGANMPKDNIDRAIKKGTGELEAAVYEELVYEAYAPGGIAILIEVL
                     TDNKNRAAAEVRNILTRSGGQLATSNAVLRLFARKGVISLDGERYSEDKVMELALEAG
                     ADDIVNEDGELVVYTSPEAFEAVLNAVNAAGMETDGAEISMIPETYVTPEGDNGGKIQ
                     KLIDKLEESDDVQNVYHNAELPEEAEE"
     gene            1541..2074
                     /gene="ruvC"
                     /locus_tag="PLW80_01500"
     CDS             1541..2074
                     /gene="ruvC"
                     /locus_tag="PLW80_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPB65207.1"
                     /translation="MPGGTERLRVLGIDPGLAAVGWGVVDLDGARVVYRGHGCFVTKP
                     GVGMAERLALIRDALKDVIAEYGPSEAAMEALYFSRNVTSALAVAEARGVIRLACRDA
                     GLSMSEYGPMDVKKAATGSGRSGKDDVQSFVRLVLGLSAIPKPDHAADALAVALCHCQ
                     GAGVRSALVDASRPGRL"
     gene            2082..2675
                     /gene="ruvA"
                     /locus_tag="PLW80_01505"
     CDS             2082..2675
                     /gene="ruvA"
                     /locus_tag="PLW80_01505"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPB65208.1"
                     /translation="MFNAIRGTLCHKGADTIRIDTNGVEWEFSVPARSVDAFGRLGDE
                     ARVLCWLLHREDSMRLYGFPSEAERSIFIELIGVDGIGPKQALKILGGIGAGDLERAL
                     ESEDLARLESVPGLGKKTAQKLVFALKGRLPSVSGASEAAPAGPHGDIVRALVDMGYD
                     RRRVAEALARVDAGIPPDHPERERELFRLAIVELSAS"
     gene            2672..>3123
                     /locus_tag="PLW80_01510"
     CDS             2672..>3123
                     /locus_tag="PLW80_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002679608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB65209.1"
                     /translation="MSALVSGSFDPAEDERENALRPRRLTEFLGQASVKANLSVFIEA
                     ALKRSEALDHVFLMGPPGLGKTTLAGILSHELGVDFKVTSAPALEKPKDLAGILTTLA
                     PRSIFFIDEIHRLKPAIEEMLYMAMEDGELDFIIGQGPAARTLRIPLPP"
BASE COUNT          602 a         1011 c          981 g          529 t
ORIGIN      
        1 gtaacggcac gaggcccgtc tcgggatgta tgatctcgtc ggcgtagaca tgctgcagct
       61 tgacgcaatc cgccccagcc tcgaccgcgg cggcggccag ttccatcgcc ttgagctcgt
      121 cgccaccgtg gccggtgccc agctcggcga tgatcaaggc ctcgccggcc gcgttcaaga
      181 cgcgatcgaa tacgtttccg ctcatcttag ctccgttcca gcgccagaac gctattcctc
      241 tcgacaatcg acgccctata ctatagaatc gtcggcatcg acagcaatcg aggagagtca
      301 atatgaagca cttattctgc gatgtctgca agaaagaagt agtcgatccc atcccgacga
      361 ggacgttctt ccacatacgc gagttcgatc tatgcgaggc ctgccatgac gacctcgagg
      421 cggcgatcaa gtatactgtg cgcggaaaga agccgttcga cttctcctgg tacgataaga
      481 tgcgcataga actcgtggaa gacggcgtca agaagaacaa gatctcttcc tccaagactc
      541 agcgctgagt cgacgacctt ggccacggtt ccgctctccg cgaggccgtg gcttgtagcc
      601 gagtttgttc tgccgttttc tgcgctttta gctcctttta cgctatatag tccaatcgtt
      661 gacagtaaat cgcggcttgt gctagtatta cgctcgcaaa aaatgaacgg atcatcccgt
      721 tttcataggc tttccagcct cggaagcgcg tatcgtcgat cggttcgcaa ccgcgtattc
      781 tacacggcaa ggagtcacaa tgtcaggaca tagcaaatgg gcgacgatca aacacaagaa
      841 gggcgcggcc gacgctaagc gcggccagat gttcaccaag ctgatcaagg aaatctcgat
      901 cgcggcgcgc atgggcggcg gcgatcccga taccaacgcc cgcctcagga ccgccgtcct
      961 caaggcccgc ggcgctaaca tgcccaagga caacatcgac cgggccatca agaagggcac
     1021 cggcgaactc gaggccgcgg tctacgagga gctggtctac gaggcctacg cccccggcgg
     1081 catcgccatc ctgatcgaag tcctgaccga caataagaat cgcgctgccg ccgaggtgcg
     1141 caatatcctg acccgttccg gcggccagct ggccacctcc aacgcggtac tccggctgtt
     1201 cgcccgcaag ggcgtcatat cgctcgacgg ggagcggtac agcgaggaca aggtcatgga
     1261 gctcgccctc gaggccggcg ccgacgacat cgtcaacgag gacggtgaac tcgtcgtcta
     1321 taccagcccg gaggccttcg aggccgtgct gaacgcggtc aacgcggccg gcatggaaac
     1381 cgacggcgct gagatatcca tgataccgga gacctacgtt acgcccgagg gcgataacgg
     1441 gggcaagatc cagaagctca tcgacaagct cgaggaatcc gacgacgtac agaacgtcta
     1501 ccataacgct gagctccctg aggaagccga ggagtagtcc atgccgggcg gaaccgaaag
     1561 gctccgcgtc ctcggcatag accccggcct cgcggccgtg ggatggggcg tcgtcgacct
     1621 agacggcgcc cgcgtcgtat acaggggaca cggctgcttc gtaaccaagc cgggcgtggg
     1681 gatggccgag cgcctggcgt tgatcaggga tgcgctcaag gacgtgatcg ccgagtacgg
     1741 gccgtcggag gccgcgatgg aggccctata cttctcacgc aacgtgacga gcgcgctcgc
     1801 cgtcgcggag gctcgaggcg tgatccggct cgcctgccgc gatgccggcc tttccatgag
     1861 cgagtacggc ccgatggacg tcaagaaggc ggcgaccggc tcggggcgct cgggcaagga
     1921 cgacgtccag tccttcgtcc ggctggtgct cggcctgtcg gccatcccga agcccgacca
     1981 cgccgccgac gcgctcgcgg tggcgttatg ccattgccag ggagccggcg tgcgctcggc
     2041 cttggttgat gcatcgcgcc ccggccgact ataatccggc tatgttcaac gctattcgcg
     2101 gcacgctctg ccataagggc gccgacacga tcaggataga caccaatggc gtcgagtggg
     2161 aattctcggt gccggctcgc tcggtcgacg ccttcgggcg gctcggcgac gaggcgcgcg
     2221 ttctatgctg gctactccac cgcgaggatt cgatgcgcct ctatggcttc ccctccgagg
     2281 cggagcgctc gatcttcatc gagctgatag gcgtcgacgg catcgggccg aagcaggccc
     2341 tcaagatact tggcggcatc ggggctggcg acctcgagcg cgcgctcgag agcgaagacc
     2401 tggccaggct cgagtccgtc cccggacttg gcaagaagac ggcccagaag ctagtcttcg
     2461 ccctcaaggg caggttgccg tcggtatcgg gagcatcgga ggccgccccc gccgggccgc
     2521 atggcgacat cgtgcgggcc ctcgtcgaca tgggctacga taggcgccgg gtcgccgagg
     2581 cgctggcccg cgtcgacgcc ggcattcccc ccgaccatcc cgagcgcgaa cgggagctgt
     2641 tcaggctcgc gatcgtcgag ctgagcgcct cgtgagtgcc ctggtatccg gctcgttcga
     2701 cccggccgag gacgaacggg agaacgcgct caggccccgg cgcctgaccg agttcctcgg
     2761 gcaggcctcg gtcaaggcca acctgtcggt cttcatcgag gcggccctga agcgcagcga
     2821 ggcgctcgac cacgtgttct tgatggggcc gcccggcctc ggcaagacga cgctcgccgg
     2881 catcctgtcg cacgagctcg gcgtcgactt caaggtaacg agcgccccgg cactcgagaa
     2941 gcccaaggac ctggccggca tactgacgac cctcgcgccc agatcgatct tcttcatcga
     3001 cgagatccat aggctcaaac cggcgatcga ggagatgctg tacatggcga tggaggacgg
     3061 cgagctcgac ttcatcatcg gccagggacc ggcggccagg actctgcgca tcccgctgcc
     3121 ccc
//
LOCUS       DAOOLN010000075         3121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_37880_length_3121_cov_4.053816, whole genome shotgun sequence.
ACCESSION   DAOOLN010000075 DAOOLN010000000
VERSION     DAOOLN010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37880_length_3121_cov_4.053816"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="PLW80_01515"
     CDS             <1..857
                     /locus_tag="PLW80_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB65210.1"
                     /translation="PGLAKTLAVRTFAAAAGLSYRRIQFTPDLLPGDITGTLFYDAAS
                     GAFVPRRGPVFANIVLADEINRAPAKVQSALLEAMEERQVTMGDHSLPLPEPFFVLAT
                     QNPIEHEGTFRLPEAQLDRFMLKVVVDYPDEAEELDVVLRYADRPAGKADEAAVQPVI
                     GQAEIAALKAAAASVRFERDAAAYAVAVVRATRPSRDGKQAAGALRELSRYIEYGASP
                     RGSIALYRAARAAALLAGRDYVLPDDVKRSAPAALRHRVIPSYEAEADRVGPDAIVKG
                     VLSAVPVP"
     gene            857..1765
                     /locus_tag="PLW80_01520"
     CDS             857..1765
                     /locus_tag="PLW80_01520"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPB65211.1"
                     /translation="MDDASLRERIRALVFAAPALSEALASGGFRSVFKGRGMDFEALR
                     EYEATDDALRMDWNATARFGRPFVKTYKDDRDLTLYLIVDESRSMDFGAGRAKRETAA
                     LAASLLAYACSLNGIRVGALFFGGAAAMDGRAPASGQGAARALMERLAAGPASAAGPA
                     SAAGPARFGSELGAALTAASSILKRRSLVLVVSDFMCSGYALPLALLARRNDVAALRV
                     HDRLDSAPPALGLTLRSADAESGERRLVTPRSAAYRRERERWAKAARLEWLVALSAAK
                     VPYLEMDSSTDPVEELIAFFGRRRSA"
     gene            1765..2697
                     /locus_tag="PLW80_01525"
     CDS             1765..2697
                     /locus_tag="PLW80_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65212.1"
                     /translation="MGVIGRRPRRRVGGPLVGAALAVALLAAAPTGARAEAAEASILR
                     VEFDPPTFYPGQATLAYATLDPGTASWSEAVLAVVSERPDEAGAPETGAPEVEILSAR
                     LERRGGQPILIVRFVPWTAGRGLIPGMELGGLAVTGLRFDCASALTSLRPPVLLPQLE
                     PPGLRSRLYLAGGVALVALLAGLTAAFRAAPWFGAIKARWAAARSRKEFEALLEAVVE
                     IEPETAAWAALCAAVRRFVGTRTGVDWSAMTASEIALSPEGPVPGPTRDAAAAILSSG
                     DAARFAGSVEAGLADAVAAARAVADSLDEAGRAP"
     gene            2694..>3121
                     /locus_tag="PLW80_01530"
     CDS             2694..>3121
                     /locus_tag="PLW80_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPB65213.1"
                     /translation="MIAFDHPLALALCLAPVAAAVAGARGRNRSRLVVLPLDVWGGAI
                     SAEAPAPWRAARAASALLFALAWMALSVAAAGPASVAESAGRANAGADVVFVLDASPS
                     MAARDLEPTRLEAAKAFVLSWLGSPEGASGAAVGLVAFGA"
BASE COUNT          346 a         1191 c         1137 g          447 t
ORIGIN      
        1 tgcccggcct ggccaagacc ctggcggtca ggaccttcgc tgccgcggcc ggcctatcgt
       61 accgccgcat ccagttcacg cccgatctcc tgcccggcga catcaccggc accctgttct
      121 acgacgccgc ctccggcgcc ttcgtcccgc ggcgcggccc ggtcttcgcg aacatcgtgc
      181 tggccgacga gatcaaccgc gcgcccgcca aggtgcagtc ggccctgctg gaggcgatgg
      241 aggagcgcca ggtgacgatg ggcgaccaca gcctgcccct gcccgagccg ttcttcgtgc
      301 tggcgacgca gaacccgatc gaacacgagg gcaccttcag gctgccggag gcccagctgg
      361 accgcttcat gctcaaggtc gtcgtcgact accccgacga ggccgaggag ctcgacgtgg
      421 tgctgcgcta cgccgatcgt ccagccggca aggccgacga ggcggccgtc cagccggtca
      481 tcgggcaggc ggagatcgcc gcgctcaagg cggcggccgc gtcggtgcgc ttcgagcggg
      541 acgccgccgc ctacgccgtc gccgtggtcc gggccacgag gccgtcgcgg gacggcaagc
      601 aggccgccgg cgccctgcgc gagctgtcgc gctacatcga gtacggcgcc agcccgcgcg
      661 gctcgattgc gctgtaccgc gcggcccggg ccgccgcgct gctcgccggc cgcgactacg
      721 tgctgcccga cgacgtcaag cgatccgctc cggcggcgct acgccaccgc gtcatcccgt
      781 cctacgaggc ggaggccgac cgggtcgggc cggacgccat cgtcaagggc gtgctgtcgg
      841 ccgtgccggt gccctgatgg acgacgcttc gctgcgcgag cggatacgcg ccctcgtgtt
      901 cgccgcgccg gccctgtccg aggcgctcgc ctcgggcggc ttcaggtcgg tgttcaaggg
      961 gcgcggcatg gacttcgagg cgctccgcga gtacgaggct accgatgacg cgctgcgcat
     1021 ggactggaac gcgaccgcgc gcttcggtcg gcccttcgtc aagacctata aggacgaccg
     1081 cgacctgacg ctctacctga tcgtcgacga gtcccgctcg atggacttcg gcgccggccg
     1141 ggccaagcgc gagaccgccg ccctggccgc ctcgctcctg gcctacgcct gctcgctcaa
     1201 cggcatcagg gtgggcgcgc tgttcttcgg cggcgccgcc gcgatggacg gccgcgcgcc
     1261 ggcctcgggc cagggagccg cccgggcgct catggaacgt ctcgccgcgg ggccggcgag
     1321 cgccgcgggg ccggcgagcg ccgcggggcc ggcgcggttc gggtcagaac tcggcgcggc
     1381 cctgaccgcc gcctcgtcca tcctgaaacg ccgtagcctc gtcctcgtcg tctcggactt
     1441 catgtgctcg ggctacgccc tgcccctggc cctgctggcc aggcgcaacg acgtcgcggc
     1501 cctgcgcgtg cacgaccgcc tggactcggc cccgcccgcc ctcggcctga cgctgcgatc
     1561 ggccgacgcc gagtcgggcg agcggcgcct cgtgacgccc cgctcggccg cgtaccgccg
     1621 cgagcgggag cgatgggcca aggccgcccg gctcgagtgg ctcgtggcgc tgtccgcggc
     1681 caaggtgccg tatctcgaga tggactcgtc caccgacccg gtcgaggagc tcatcgcctt
     1741 tttcggccgg aggaggagcg cctgatgggc gtcatcgggc ggcgcccccg ccgccgcgtc
     1801 ggcggcccgc tcgtgggcgc ggcgctcgcc gtagccctcc tggccgccgc gccgacgggc
     1861 gctcgcgccg aggcggcgga ggcttctatc ctgagagtcg agttcgaccc tcctaccttc
     1921 taccccgggc aggcgacgct ggcctacgcg accctcgatc ctggaacggc ttcctggagc
     1981 gaggccgtcc tggccgtcgt ctccgagcgg cccgacgagg ccggggctcc cgagaccggg
     2041 gctcccgagg tcgagatcct gtcggcccgg ctcgagcgtc gaggcggcca gccgatactg
     2101 atcgtccgct tcgtcccgtg gacggcgggc cggggcctca tcccgggcat ggagcttggc
     2161 ggcctcgccg tgacgggcct acgcttcgac tgcgccagcg ccctgaccag cctgcggcct
     2221 cccgtcttgc tgccccagct cgagcctccg gggctccgct cgcggctcta cctcgccgga
     2281 ggcgtcgccc tggtcgcgct gctcgccggc ctgaccgcgg ccttccgcgc cgcgccctgg
     2341 ttcggcgcca tcaaggcgcg atgggccgcc gcgaggagcc ggaaggaatt cgaggctcta
     2401 ctggaagcgg tcgtcgagat cgagccggag acggccgcct gggccgcgct ctgcgccgcc
     2461 gtcaggcgtt tcgtcgggac caggaccggc gtcgactgga gcgccatgac ggcgtccgag
     2521 atagcgctct ccccggaggg ccccgtgccg ggtcctaccc gcgacgccgc ggccgcgatc
     2581 ctttcgtccg gggacgccgc ccgtttcgcg ggctcggtcg aagccggcct ggctgacgcc
     2641 gtcgcagcgg cccgcgcggt cgcggacagc ctcgacgagg cggggagggc gccgtgatag
     2701 ccttcgacca tccgctcgcg cttgcgctgt gcctggcccc ggtggccgcc gccgtggcgg
     2761 gggcccgagg gcggaaccgg agccgtctcg tagtcttgcc gctcgacgtc tggggcggcg
     2821 ccatcagcgc cgaagcgcct gcgccttggc gggccgcgcg cgcggcctcg gcgctgctgt
     2881 tcgccctggc ctggatggcg ctgtcggtag ccgccgccgg cccggcctcc gtcgcggaat
     2941 cggccggccg cgccaacgcc ggagccgatg tggtgttcgt gctcgacgct tcgccgagca
     3001 tggccgcccg agacctcgag ccgaccaggc tggaggccgc caaggctttc gtgctgtcct
     3061 ggctcggttc gcctgagggc gcgtcggggg ccgcggtcgg cctggtggcg ttcggggccg
     3121 a
//
LOCUS       DAOOLN010000076         3116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_37970_length_3116_cov_2.941522, whole genome shotgun sequence.
ACCESSION   DAOOLN010000076 DAOOLN010000000
VERSION     DAOOLN010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37970_length_3116_cov_2.941522"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..1076
                     /locus_tag="PLW80_01535"
                     /pseudo
     CDS             66..1076
                     /locus_tag="PLW80_01535"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1073..2011
                     /locus_tag="PLW80_01540"
     CDS             1073..2011
                     /locus_tag="PLW80_01540"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HPB65214.1"
                     /translation="MIDYTKRVRLVAAADAALALLVSLGLILSPTSSGSKESRRDLLD
                     DASAVTSIRISGGEPVELVKTGTGWEAVDGDAWLPVDSSRVAAFLKAVDSIERAELVA
                     EDASSQPALGLGAGARKVTLLGEGGKELCAFALGSYASSPGTVYISIGDGPESYAVAS
                     GMASYALGRRSSWLDLRAWSSPPTPDDVEEVLLRGSLDGAGGTAVSLDYMIARSGKGW
                     MSGGEALDAMKVDAMIRAIAALRGDDYADADEPAGNAVVELELRLGNGRTLNLAVEPV
                     RADGRYPARSSQRERGMYLPAWSLTEALKPIGELRP"
     gene            complement(2012..>3116)
                     /locus_tag="PLW80_01545"
     CDS             complement(2012..>3116)
                     /locus_tag="PLW80_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPB65215.1"
                     /translation="AKVADAWAAQPNLLAVVIRDPDGEVLYARPGSSPYYARSDEGLV
                     FERPEGTTTRFSGPISAGMSVEALYVTLTQEAAFYPIRDGAVALASLLALLVAWLLAA
                     ANAKPEATNESLRESPEAVTDDWEPSIPELIPPESSLYAEDPFGDSTSSPEPAADEPA
                     AYEPVAEDAAADEPTARTAPPGPKSAFIGQNDLPEGCLEGPRGLFDAETGLGWEAYLR
                     ERLSAELKRSASFEQDLSLLIASIDGAERGDEEFNLFASSVRDYFTFKDMAFLFGDNG
                     AALILPNMDVDHALRMCGELMKKLSASMAVRRAGGTGVYMGLSSRAGRLVDADRIIGE
                     SMAALSKARSDGQGGIMAFRPDPEKFRAYLAGQ"
BASE COUNT          463 a         1018 c         1092 g          543 t
ORIGIN      
        1 acgccgataa gagcctgggg ggggactagc aggcgctacg gggcaacctc gcgagggccg
       61 gctacgagac gcgcgagctg gagcgcggcc gggccgtcga cgacgacgtc gatgtgctgt
      121 tcgtgctcgg caacgcggcg atggatcgtt acgactcggc tttcgtagac gccttcgtca
      181 tgcgcggggg caagaccttc ttcgcggtca agggtgtcga cgtgcgggcc gactacgggc
      241 tggcggcgtc cgcggtaccg gacggcggcc tcttgtcgat gctgtcgtcc tacggcttcg
      301 acgtggatcg ctcgctggtc ctcgaccagt cgagccttac cgtgccgttc cagacccagg
      361 ggccgaccgg ctcgtaccag atccagtacc tgcgctaccc gttctggatc atcgtcgacg
      421 agcgtttcgt atcggacgac tcgccgatga cggcccgctt cggcggtctc gacctgttct
      481 ggccgagccc gctacgcctc cgacccgtcg aaggcgtcga gtacgtcgag ctggccaaga
      541 ccagcccgaa ggcctggctg cagaccgagc gcttcgccgc cggtccggac gaccaggccc
      601 tgtatgcgct cgagcgcgac ggcacgggcg gtcagtacct gctcgcggcc tcggcgagcg
      661 gatccttcag gagcgccttc tcggccggag atccgccgta cagggacgga gccccgccgc
      721 tagcgccgac gagagtcgag tccgagccga cacgtatggt cgtcgtctcg tccgcagact
      781 tcctaagcga cctgatgcgc atgagcgact cgggcttcaa cgcgtcgttc gcgctgaatg
      841 cggccgattg gctcgcctcg agcgacgact tgatcgcgat ccggtccagg gccgtcgccg
      901 acacgcggct caaccgcgtc gacgacgacg gcctgcggga tttcctggtc gccgttacct
      961 atatagtctg cctcggcatc gtgccattcg gcgtcgtggc cttcgccttt gcgcgctcgg
     1021 cgcgcaggcg cggcgccgaa gccgcctcga ggccttcgag gggaggagcg gcatgatcga
     1081 ctatacgaag cgcgtacggc tcgtcgccgc ggccgacgcg gcgctggccc tgctcgtgtc
     1141 gctcggcttg atactatccc cgacttcgtc tggctcgaag gagtcgcggc gcgacctcct
     1201 cgatgacgcg tctgccgtaa cctcgatacg catatccggg ggcgagccag tcgagctcgt
     1261 caagacaggt accggctggg aagcggtcga cggcgacgcg tggctaccgg tggattcatc
     1321 ccgtgtggct gcctttctga aggcggtcga ttcgatcgag cgggccgagc ttgtcgccga
     1381 ggacgcttcg tcgcagccgg ccctcggcct cggcgcggga gcgcgcaagg tgactctgct
     1441 gggcgaaggc gggaaggagc tatgcgcgtt cgccctcggt tcgtacgctt cgtcgcccgg
     1501 tacggtctat atatcgatag gcgatgggcc ggagtcgtac gccgtcgcct ccggcatggc
     1561 ctcctacgcg ctcggccgtc gctcgagctg gctcgacctt cgggcctggt cgtcgccccc
     1621 gacccccgac gacgtcgagg aggtactgct ccgcggctcg ctcgatggcg ctggcggcac
     1681 agccgtgagc ctcgattata tgattgcgag atccggaaag ggctggatga gcggcggcga
     1741 ggctctcgac gcaatgaagg tcgacgcgat gattcgggcg atcgccgcgc tacgcggcga
     1801 cgattacgcc gacgccgacg aacccgcggg aaacgcggtc gtcgagctgg agctgcgact
     1861 cgggaacggc aggacgctga acctggccgt cgagccggtt cgcgccgatg ggcgctatcc
     1921 ggcccggtcg tcgcagcggg agcgcgggat gtacctgccg gcctggagcc ttaccgaggc
     1981 cctcaagccg atcggggagc tacggcccta gctattgtcc ggcgagatag gcgcggaact
     2041 tctcaggatc ggggcggaag gccatgatgc cgccctggcc gtcggagcgc gccttggaca
     2101 gggcggccat cgactcgccg atgatacggt cggcgtcgac gaggcgtcca gccctcgagg
     2161 acaggcccat gtagacgccg gtcccgccgg ctcgcctgac cgccatcgac gcggacagtt
     2221 tcttcatcag ctcgccgcac attctgagcg cgtggtccac gtccatgttc ggcaggatga
     2281 gcgcggctcc gttgtccccg aacaggaaag ccatgtcctt gaaggtaaag taatcgcgca
     2341 cggacgacgc gaagaggttg aactcctcgt cgccgcgctc ggccccgtca atggacgcga
     2401 tcagcagtga taggtcctgc tcgaacgagg cggagcgctt gagctcggcg ctgagtcgct
     2461 ctcgcaggta ggcttcccag ccgaggccgg tctcggcgtc gaacagtccg cgtgggccct
     2521 cgaggcaacc ttccggcagg tcgttttggc cgatgaacgc ggacttcggc ccgggaggag
     2581 cggttctcgc ggtcggctcg tcggccgcgg catcttcggc cacgggttcg taggccgcgg
     2641 gttcgtcggc cgctggttcg ggcgatgatg tgctatcgcc gaacgggtcc tcggcgtata
     2701 gcgacgactc gggcggtatg agctccggaa tgcttggctc ccaatcgtcg gtaacggctt
     2761 ccggcgactc gcggaggctc tcgttcgtag cttccggctt ggcgttcgcc gccgcgagga
     2821 gccatgcgac cagtagggcc aggagcgagg cgagggccac ggctccgtcg cggatcgggt
     2881 agaatgcggc ttcctgcgtc agggtcacgt agagcgcctc gacgctcatg cccgcggaga
     2941 tggggccgct gaaacgcgtg gtcgtgcctt cgggacgctc gaagactagc ccctcgtcgc
     3001 ttcgagcgta gtatggtgac gagcccggtc tggcgtacag gacctcgccg tccgggtctc
     3061 tgatgacgac ggccaaaagg ttcggttgag ccgcccacgc gtcggcgacc ttggcc
//
LOCUS       DAOOLN010000077         3114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_38006_length_3114_cov_2.720170, whole genome shotgun sequence.
ACCESSION   DAOOLN010000077 DAOOLN010000000
VERSION     DAOOLN010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38006_length_3114_cov_2.720170"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..378)
                     /locus_tag="PLW80_01550"
     CDS             complement(151..378)
                     /locus_tag="PLW80_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65216.1"
                     /translation="MTFNALYESTFEVELAGAELAGLYVSLTAAEERLDRNQLQALDR
                     LRSVLYENLSVEELESLPGLYTAAAGPGGSA"
     gene            complement(368..1303)
                     /locus_tag="PLW80_01555"
     CDS             complement(368..1303)
                     /locus_tag="PLW80_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB65217.1"
                     /translation="MKIRFWGARGSLPTPLTPAKLRSKISSIVQRITPADLESQESRE
                     IFLARLPDYLFSTVGGNTACVEVITSEPCRIVFDAGTGIHNLGNEITAQRKPADVHLF
                     FSHFHWDHIQGFPFFGPAFDPRTRLRLYSPDPRLREILEGQMKAPYFPVGLDDMAAGK
                     EFIRLESGVDIGGVRIMHRAMSHPGGCYSYAVEEAGKKAIYSTDTELDPKDFNRTPEN
                     ESYFRGADALIIDAQYTLGEAIEKQYWGHSSFSLAADFAASWNIKRLVLFHHEPAYGD
                     QKIGSILKSASWYLKHIEDHGIEVVLAREGVEIDV"
     gene            complement(1817..1898)
                     /locus_tag="PLW80_01560"
     tRNA            complement(1817..1898)
                     /locus_tag="PLW80_01560"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1862..1864),aa:Tyr,seq:gta)
     gene            complement(1931..2003)
                     /locus_tag="PLW80_01565"
     tRNA            complement(1931..2003)
                     /locus_tag="PLW80_01565"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1968..1970),aa:Thr,seq:tgt)
     gene            complement(2064..>3114)
                     /locus_tag="PLW80_01570"
     CDS             complement(2064..>3114)
                     /locus_tag="PLW80_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65218.1"
                     /translation="PESAATIELYRSERKRPRILSAAFDANGELMAADFDASEIAYLS
                     DPASRFAGLSVEVGRVYSDSFPRVSLDVTVKDRHGRPVTGLGYPNFYISELVKTKERR
                     VEGDKPVDYIASSIRPAGELSFDGSLDQSSGIDLTFLLEGSPQLSPMRLEARDAAYAV
                     YESLGGGTDSMARLVVAGRVAQPPSAANVAAIGAAALGYEPSSDWRFDSGLRLAAGTL
                     FGSSRRKAVVYLGTGSVNEPYLDGSSLAELASLLENNCISLYAVIVGKGGPSEALSFL
                     VERSGGSVYRADRPEGLAVVTASIRQARTGRYRLSYLSSADDGFGRSYLPFSVEVYLR
                     DRSGKDEGGFFAPIR"
BASE COUNT          576 a          972 c          993 g          573 t
ORIGIN      
        1 cctcgcctgt cggcctgtcg tagcgttccg ccagcgcgat gacgccgagc ccgagggcca
       61 cggcggcgat gacgaggagc gcgacgacga cgaaggcggc cttggcggcc ttgccgaccg
      121 cgccagttcc ctggaccttg gtacgtcggc tcatgccgat ccccccgggc cggcggccgc
      181 ggtatagagc ccgggaaggc tctcgagctc ctctaccgac agattctcgt ataggacgga
      241 cctgaggcgg tcgagggcct ggagctggtt cctgtcgagc cgttcctcgg cggctgtcag
      301 cgagacgtag aggcccgcca gctcagctcc ggccagctcg acttcgaatg tagactcgta
      361 cagggcgtta aacgtcaatc tctacgcctt ccctcgccag gaccacctct atgccgtggt
      421 cttcgatatg cttaaggtac catgacgctg acttgaggat cgagcctatc ttctggtcgc
      481 cgtaggccgg ctcgtgatgg aacaggacaa gccgcttgat gttccatgag gccgcgaagt
      541 cggccgccag gctgaacgac gaatggcccc agtattgctt ctctatcgcc tcgccgagcg
      601 tgtactgggc gtcgatgatc agggcgtccg cgcccctgaa gtacgactcg ttttccggcg
      661 tccgattgaa gtccttgggg tcgagctcgg tatcggtcga gtagatggcc ttcttgccgg
      721 cttcctcgac ggcgtagcta tagcaaccgc cgggatggct catcgcgcgg tgcatgatgc
      781 gtacgccgcc tatgtcgacg ccgctctcga gccggatgaa ttccttgccg gcggccatgt
      841 cgtcaagccc gacggggaag tacggcgcct tcatctgccc ctcgagtatc tcgcgtagcc
      901 tggggtcggg actgtacagc cgtagcctcg tcctcgggtc gaaggccggc ccgaagaacg
      961 ggaagccctg tatatggtcc caatggaagt gcgagaagaa caagtggacg tccgccggct
     1021 tccgctgagc cgtgatctcg ttcccgagat tatggatgcc ggtgccggcg tcgaacacga
     1081 tgcggcaagg ttctgaggtg atcacctcga cgcaggcggt attgccaccg acggttgaaa
     1141 acaggtagtc gggtagccgc gcgaggaata tctcacgcga ctcctgcgac tccaggtcgg
     1201 cgggcgttat gcgctggact atcgaggata tcttgctacg tagcttggcc ggggtcagcg
     1261 gcgtcggaag cgagccgcgg gctccccaaa agcgaatctt catcgatcgt ctccaatgcc
     1321 gcgccgtacg gtatgacgag ggtccggttc gcccttttgc gagcgaaccg ctcggctatc
     1381 tcccctgctc cgatgatcgg ccccttgccg atgccggggc aggagccggc ttcgtcttgc
     1441 cagtcatcat ggctatcgag gatgcctggc cagaaggggt acgtcgcgca ctgtatcggt
     1501 ctcgactcgt acgcggcgca gcctccgtcg ctccatagta tacaatcatt attcggcttt
     1561 tccttcaatg aaatcgccag ccccgtgccg atgtcgacgg tacgcaggaa cgctccgacg
     1621 aacgccctga acggaagccc cgaacgcgcg tgtaggcgtc gcaggtcgtc ggctgataga
     1681 aagacatacc ccggctcgta cctgcagcac gctgagcacc tggcgcactc gaagcgtagc
     1741 ccctcgtgcc agaacgattg gttcatcgaa gtcctcgcat atgaagaagg accgccagac
     1801 ggcggtcctt gaaggatgga gagagaagga tttgaacctt cgaaggctta gccaacagat
     1861 ttacagtctg ccccctttga ccactcggga atctctccag ggtaaatccg atcgccaccg
     1921 gactttcttg agccatctcc cggaatcgaa ccggggacct cgagattaca agtcacgcgc
     1981 tctgccaact gagctaagat ggcgccgaac gagcgctact atacagaacg aacgggagcg
     2041 cgtcaagccg ggcgataaag aaactatcgg atcggcgcga agaatccgcc ctcgtctttc
     2101 ccggacctgt cgcgtaggta gacctccaca ctgaacggca ggtagcttct gccgaagcca
     2161 tcgtccgctg acgataggta ggataggcgg tagcggcccg ttcgggcctg gcggatactg
     2221 gctgtgacga ccgccagccc ttctggccta tcggcccggt acaccgagcc gccgctccgt
     2281 tcgaccagga aggacagggc ctcggacggg cctcccttgc cgacgataac ggcgtagagc
     2341 gagatgcagt tattctcgag aagcgatgcg agctcggcga gtgacgatcc gtccaggtat
     2401 ggctcgttga ccgagccggt gcctaggtag accacggctt tgcgcctgga cgagccgaac
     2461 agggtgcctg ccgcgagccg aaggcccgaa tcgaagcgcc agtccgacga tggctcgtag
     2521 ccgagggccg ccgcccctat ggccgcgacg ttggcggcgg aaggcggctg ggcgacgcgg
     2581 ccggccacga cgagcctggc catcgaatcc gtcccgccgc cgagcgactc gtagacggcg
     2641 taggcggcgt ccctcgcctc gagccgcatc ggtgagagct ggggcgaacc ctcgaggagg
     2701 aaggtcaggt cgatgccgct cgattggtcg agcgagccgt cgaacgacag ctcgccagcc
     2761 ggacgtatcg acgaagcgat ataatcgacg ggcttatctc cctcgacgcg tcgttccttg
     2821 gtcttcacga gttccgagat atagaagttc gggtagccga gccccgtgac aggcctgccg
     2881 tgcctatcct tgacggtaac gtcgaggctg acgcgaggga aggagtcgga gtacaccctg
     2941 ccgacctcga ccgacagccc ggcgaacctg gaggccgggt ccgataggta ggcgatctcg
     3001 gaggcgtcga agtcggccgc cataagttcg ccgttggcat cgaacgccgc agacaggatg
     3061 cgcggcctct tgcgctccga ccggtatagc tctatcgtcg ctgcgctctc gggg
//
LOCUS       DAOOLN010000078         3109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_38088_length_3109_cov_2.343157, whole genome shotgun sequence.
ACCESSION   DAOOLN010000078 DAOOLN010000000
VERSION     DAOOLN010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38088_length_3109_cov_2.343157"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLW80_01575"
     CDS             <1..202
                     /locus_tag="PLW80_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65219.1"
                     /translation="QEGKDALAWSSTEGYAPGSVYDELYSGEERASWVYYGWNPLLRP
                     DGERWAFDISCLYAYVGFSMFF"
     gene            290..1366
                     /gene="mtnA"
                     /locus_tag="PLW80_01580"
     CDS             290..1366
                     /gene="mtnA"
                     /locus_tag="PLW80_01580"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPB65220.1"
                     /translation="MSQIIAMEYAEDRLRLVDQRRLPGSYEFFECRTYRDCAFAIKDM
                     VVRGAPAIGAAAAYGAALAASEFSSLGAAEFDPAMRVALDELRRARPTAVNLSWAVDR
                     MACALDKAIAGGTGPGRTLYERLKAEADAILADETARSERLSEHGNALIPDGAAILTH
                     CNTGSLAVPGPGTALGMIKAAHRAGKGVSVYADETRPRLQGARLTAWELDREGVPVTL
                     IADSVAATLMRDGKIGLVVVGADRIAANGDAANKIGTFMLSVLAARYGVPFYVAAPVS
                     TIDFACPSGDAIEIEERPADEVTTIEGVRVAPDGIHVYNPAFDVTPAEHITAIVTERG
                     VLRPPFAEAIAALRATPGVGEGVL"
     gene            1363..2547
                     /locus_tag="PLW80_01585"
     CDS             1363..2547
                     /locus_tag="PLW80_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPB65221.1"
                     /translation="MRSDRGGFDTRAIHAGGGVNPERALCPPIFMTSSFTFDDLAAAD
                     DVMSFRSDDYVYTRGNNPTLRLFEARMASLEGGSGAVAFASGMAAISSTLLSLLKPGD
                     ELVAHRTLYGSAYSFVSTLLPRYGIACRFVDLTDPRAVETALGERTRVVYFETPSNPN
                     LDIVDIRLVGRAAAARGARIVVDNTFATPYLQRPLELGADVVVHSATKYICGHGDAVA
                     GVAVAKDPDYVKTLKFGYMCELGGVLSPFNAWLLLRGLKTLGLRMRQHCANAERVAAF
                     LADHPKVQRVFYPGLEGSPGRETAMSQMSGFGAIISFEVKGGLAAASRAVDAAELFPI
                     AVSLGDCETLIQLPAAMTHRGYDRERLAEFGLTESMVRLSVGLEDSDDIVAELDRMLA
                     EA"
     gene            2574..2948
                     /locus_tag="PLW80_01590"
     CDS             2574..2948
                     /locus_tag="PLW80_01590"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB65222.1"
                     /translation="MRTLIRNGTILTMEGPEPELVFGDLGLDGSRIAFIGPSPAGFSP
                     ERTIDAAGGVVMPGLVDAHTHIAMSLLRHYADDLPFWNWLFERVLPAEEKLCGRYVYD
                     GSTLSLAEMIRGGVTCFADMHL"
BASE COUNT          507 a         1064 c         1040 g          498 t
ORIGIN      
        1 ccaggagggc aaggatgcgc tagcctggtc gtcgaccgag ggctacgcac cgggaagcgt
       61 ctatgacgag ctatactcag gcgaggagcg ggcgagctgg gtttattatg gttggaaccc
      121 gctcctgcga ccggatggcg agcgctgggc cttcgatatc agctgccttt acgcgtacgt
      181 ggggttttcg atgtttttct aaaaccccgg cctcgccgcg tttgacagag ccacccttgg
      241 gcactactat cggcatacta gctaaacccg aggggagcga acccgattca tgagccagat
      301 catagccatg gaatatgccg aggacaggtt gcgcctcgtg gaccagcgcc gcctgcccgg
      361 ctcgtacgag ttcttcgagt gccggaccta ccgcgactgc gcgttcgcga tcaaggacat
      421 ggtcgtccgc ggcgcgccag cgatcggggc cgccgctgcc tacggggcgg cgctcgccgc
      481 gtcggagttc tcgtcgctcg gggccgccga gttcgacccg gccatgcgcg tggctctcga
      541 cgagctgagg cgggccaggc cgacagcggt caacctgagc tgggccgtgg atcgcatggc
      601 ctgcgccctc gacaaggcga tcgcgggcgg gacagggccg ggccggacgc tctacgagcg
      661 gctcaaagcc gaggcggacg ccatcctggc cgacgagacg gcccgtagcg agcgcctatc
      721 ggaacatggc aacgccctca tccccgatgg tgcggcgata ctgacccact gcaatacggg
      781 ttcgctggcc gtgccggggc cgggcaccgc gctcggtatg atcaaagcgg cccaccgggc
      841 cggcaagggc gtatcggtct acgccgacga gacgcgtccg cgcctccagg gcgctcgctt
      901 gaccgcctgg gaactcgacc gcgagggcgt gccggtcacc ctgatagccg acagcgtcgc
      961 cgcgacactg atgcgggacg ggaagatcgg cctggtcgtc gtcggggccg accgtatcgc
     1021 cgccaacggt gacgcggcga acaagatagg caccttcatg ctgtcggtcc tggccgcgcg
     1081 ctacggcgtg cccttctacg tggccgcgcc cgtatcgacg atagacttcg cttgtccctc
     1141 cggcgacgcg atcgagatcg aggagcgccc agccgacgag gtgacgacta tcgagggcgt
     1201 cagggtcgcg ccggacggca tccacgtcta taacccggcc ttcgacgtga cgccggccga
     1261 gcatatcacg gccatcgtca ccgaacgcgg cgtgctccgg ccacccttcg ccgaggctat
     1321 cgcggccctg cgcgctactc ccggcgtcgg cgagggggtc ctatgagaag cgaccgcggc
     1381 ggcttcgata cccgagcgat tcacgccggc gggggcgtca acccggagcg cgcgctgtgc
     1441 ccccccatct tcatgacctc gtcgttcacc ttcgacgatc tggccgccgc tgacgacgtg
     1501 atgagcttca ggagcgatga ctatgtctac acgcgcggca acaacccgac gctcaggctg
     1561 ttcgaagcgc gcatggcgtc cctcgagggg ggctccggcg cggtggcctt cgcctccggc
     1621 atggccgcca tcagctcgac gctcctgtcg ctgctcaagc caggggacga actcgtcgcc
     1681 caccggaccc tgtacggctc ggcgtatagc ttcgtctcga cgctgttgcc tcgctacggc
     1741 atcgcctgcc gcttcgtcga cctgaccgat ccgagggccg tggagacagc cctcggcgag
     1801 cgcacccgcg tcgtctactt cgagaccccg agcaacccga acctcgacat cgtcgatatc
     1861 aggctcgtcg gccgagccgc cgccgcccgc ggcgccagga tcgtcgtcga taacaccttc
     1921 gccactccct atctgcaacg gccgctcgag ctcggcgccg acgtggtcgt ccacagcgcc
     1981 accaagtata tctgcggcca cggcgacgcg gtcgcgggcg tcgcggtcgc caaggacccc
     2041 gattacgtta agacgctcaa gttcggctac atgtgcgagc tcggcggcgt cctgagcccc
     2101 ttcaacgcct ggctcctcct ccgggggctc aagaccctcg ggctcaggat gcgccagcac
     2161 tgcgccaacg ccgagagggt agccgccttc ctggcggacc accccaaggt gcagcgcgtc
     2221 ttctatcccg ggctcgaggg tagcccggga cgcgagaccg cgatgtccca gatgagcggc
     2281 ttcggcgcca tcataagctt cgaggtcaag ggggggctgg ccgcggcctc gcgggcggtg
     2341 gacgcggccg agctattccc gatagcggtc agcctcgggg actgtgagac gctgatccag
     2401 ttgcccgccg cgatgaccca ccggggctac gaccgggagc ggctggcgga gttcggcctg
     2461 accgagagca tggtcaggct gtcggtcggc ctcgaggact cggacgatat cgttgccgag
     2521 ctcgaccgca tgctcgccga ggcctgatag gcggcgatgg acgaggagac gagatgagaa
     2581 cgctgatacg gaacggtacg atactcacga tggaaggccc cgagcccgag ctggtcttcg
     2641 gggacctggg cctagacggt agccgcatcg ccttcatagg cccttctcct gcgggcttct
     2701 cgcccgagcg gacgatagac gccgccggcg gcgtcgtgat gcccggcctc gtcgacgcgc
     2761 atacccatat agcgatgagc cttctccgcc attacgccga cgacttgccg ttctggaact
     2821 ggctattcga gcgcgtactg ccggccgagg agaagctatg cggccgctac gtctacgacg
     2881 gctcgacgct cagcctcgcc gagatgatac gcggcggggt cacctgcttc gccgacatgc
     2941 acctgtgacc cgagatcgtc gcgagcacgg ccgcggagct gcacatgcgc gcgagcgtcg
     3001 ggctcgtcgt ggtcgaatcg gcgactcgct gggacaccgg cccggacgag tacatcgaga
     3061 agggtctcgc gctgcgcgac gagtaccgcg gcgacgcgct gatcaccac
//
LOCUS       DAOOLN010000079         3099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_38248_length_3099_cov_3.279895, whole genome shotgun sequence.
ACCESSION   DAOOLN010000079 DAOOLN010000000
VERSION     DAOOLN010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38248_length_3099_cov_3.279895"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1668
                     /locus_tag="PLW80_01595"
     CDS             <1..1668
                     /locus_tag="PLW80_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65223.1"
                     /translation="SGLAGLLEALPMEAFAYLYTIIIAENELSADELAHFVSGLGLGR
                     AGAAVLRRLLVQAGLVDPFDPRTPVEPLSAEELAGVIGDDVAESLRASLSRYLVGLYR
                     SGRIRASLGFLRRVGERQDDERLICDCLFEDAMRPDKPLSDEPTFLSPSSAAVNRFWT
                     ALVARDRIASEAAAVAADERLAGPRAQAMKALVKSELAYACGDAERASKGAREAMLAL
                     GKGAPPRLEARSQRLMGLASLALDRHTEAADYLTNAQELSESAGIEYERMMAAYAKAV
                     VDFLSGAIGRSLKILLHAEEASSRLFRMDVKAAIESLRGRIDLELGAYDEAARRFGAL
                     AETATAYGMGEAAKRARIWGARAVAYAGGYDVAAGVLEAEAAAGERPDPEAMVFRGEL
                     ELLRGRPHEARAWLSAPEEPPPRAFDPADSFDWSCLFSEMEGRSVGFEAADAPLSQLR
                     TSLELFARGLDERDASCAVKLHELTRSERGAGNNPGMGTYGFFCYLLEERLPSPPVDK
                     QTVLSRSFKLLQQRAGRIEERSQRALYMEKNIWNRRLIEAARLHKFI"
     gene            1722..2612
                     /locus_tag="PLW80_01600"
     CDS             1722..2612
                     /locus_tag="PLW80_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPB65224.1"
                     /translation="MEHLEGELPAPYRRDGDLLTVMGEERTAYWTRNVWKEPFTAEFG
                     SISDAARLLKGMQRNWAHYPTRNARRGELIAAALPPLPSKPKAFPFSVPGAPMGAFTL
                     LDEHTLMASAACSSPWPNGRLDFAEDKEGPPSRAYRKLWEALVMAGAMPGPGRRCVDA
                     GACPGGWTWVLAGLGAEVVAIDRAPIDERVAAMPGVTFLKHNAFTIKPEDIGPVDWFC
                     SDVICYPPALYDWVSLWLDSGLARNFVCTIKMQGAEWDRQTVARFAAIPGSRVLHLWH
                     NKHELTWIRVLDGAGKRREA"
     gene            2619..>3099
                     /locus_tag="PLW80_01605"
     CDS             2619..>3099
                     /locus_tag="PLW80_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65225.1"
                     /translation="MKKYRLAVLVIGLPLVAAALVAIDGNFKRSIDEQIAEEATIFDS
                     RIDATTETLARFSSYIFTREVDNPEVRSLMRRAWLGDEGQRAAYRAELRSRMLSAYDL
                     MGEYHYRQLHFHLPDGTSFLRMHSPEKFGDSLVGIRATVAAVNERLSPVIAFEEGRIY
                     "
BASE COUNT          522 a         1054 c         1037 g          486 t
ORIGIN      
        1 tccgggttag ccggcctgct cgaggccctg cccatggagg cgttcgccta cctgtatacg
       61 ataatcatcg ccgagaacga gctgtcggcc gacgagctgg ctcatttcgt gagcggcctc
      121 gggctcggcc gggccggcgc ggccgtgctg cgccgcctgc tcgtgcaggc cggcctcgtc
      181 gacccgttcg acccgaggac gccggtggag ccgctatcgg ccgaggagct ggccggcgtg
      241 ataggcgacg acgtggccga gtcgctacgg gcgagcctat cgcgatacct ggtagggctc
      301 tatcgatcgg gacgcatacg ggccagcctg ggcttcttgc gcagggtcgg cgagcgacag
      361 gacgacgagc gcctgatctg cgactgcctg ttcgaggacg cgatgaggcc ggacaaaccc
      421 ctgagcgatg agcccacttt cctgtccccg tcgtccgcgg ccgtcaacag attctggacg
      481 gccctcgtcg cccgcgaccg gatcgccagc gaggcggccg cggtagcggc cgacgaacga
      541 ctggcaggcc cgcgggccca ggccatgaag gccctcgtca agtcggagct ggcctacgcc
      601 tgcggcgacg ccgagcgcgc gtcgaagggc gccagggagg cgatgctcgc gctcggcaag
      661 ggcgcgccgc cgaggctcga ggccaggagc cagcgcctga tgggcctggc ttccttggcg
      721 ctcgatcgcc ataccgaggc cgcggattac ctgacgaacg cccaggagct gtcggagagc
      781 gccggcatcg agtacgagcg aatgatggcc gcctacgcca aggccgtcgt cgatttcctc
      841 tccggcgcta taggaaggtc gcttaagata ctcctgcatg ccgaggaggc gtcgtccagg
      901 ctgttcagga tggacgtcaa ggcggctatc gagtcgctac gcggcaggat agacctcgag
      961 ctcggcgcgt acgacgaggc ggccaggcgc ttcggggcgc tcgccgagac ggctaccgcc
     1021 tacggcatgg gcgaggcggc caagcgcgcg cgtatctggg gcgccagggc cgtcgcgtac
     1081 gcgggcggct acgacgtcgc cgcgggcgtc ctcgaggccg aggccgccgc gggcgagcgc
     1141 cccgaccccg aggcgatggt cttccgcggc gagctcgagc tgctacgcgg caggccgcac
     1201 gaggcgcggg cctggctatc ggctcccgaa gagccgccgc cgcgcgcctt cgatccggcc
     1261 gactcgttcg attggtcatg cctcttctcg gagatggaag gcaggagcgt aggcttcgag
     1321 gcggccgacg cgccactatc gcagctgcgg acatccctcg agctgttcgc caggggcctc
     1381 gacgagcgcg acgcgtcctg cgccgtcaag ctccatgagc tgacgcgctc cgaacgcggc
     1441 gccggcaaca atcccggcat gggcacctac ggtttcttct gctacctgct cgaggagcgc
     1501 ctgccgtcgc cgccggtcga caagcagacc gtgttgtcgc gctcgttcaa gctgctccag
     1561 caacgagccg gtcgcatcga agaaaggtct cagcgcgctt tatatatgga aaagaatata
     1621 tggaataggc ggctgatcga agcggcgagg ttgcataaat tcatatgagc gatacgatta
     1681 cgcgactaga cgcggtctgc taccatgcgg ctcccggatt catggaacac cttgagggcg
     1741 aactgccagc gccctaccgt cgcgacggcg acctcctgac cgtcatgggc gaggagcgga
     1801 cggcctactg gacgaggaac gtctggaagg agccgttcac ggccgagttc ggctctatct
     1861 ccgacgcggc ccgcctgctc aagggcatgc agcgcaactg ggcccactac ccgacccgca
     1921 acgccaggcg cggcgagctg atagccgccg ccctgccgcc gctaccgtcc aagcccaagg
     1981 ccttcccgtt ctccgtgccc ggagcgccca tgggagcgtt cacgctgctc gacgagcata
     2041 cgctgatggc ctcggccgcc tgctcgagcc cgtggccgaa cggccgcctc gacttcgcag
     2101 aggacaagga aggccctccg agccgcgcgt accgcaagct atgggaggcc ctcgtcatgg
     2161 cgggcgccat gcccggcccc ggacggcgct gcgtcgacgc gggcgcctgc cccggcggct
     2221 ggacctgggt actggccggc ctcggcgccg aggtcgtcgc catcgaccga gcgcccatag
     2281 acgagcgggt cgccgcgatg cccggcgtca cgttcctcaa gcacaacgcg ttcacgatca
     2341 agccggaaga tatcggcccc gtcgactggt tctgctccga cgtcatctgc tacccgccgg
     2401 ccctgtacga ctgggtgtcg ctctggctcg actccggcct ggcgcgcaac ttcgtctgca
     2461 ccatcaagat gcagggcgcc gagtgggacc gccagaccgt cgcccgtttc gcggcgatcc
     2521 ccggctctcg cgtgctgcac ctctggcaca acaagcacga attgacctgg atacgcgtgc
     2581 ttgacggagc cgggaaacgg cgcgaagctt gagacgccat gaaaaaatac aggcttgccg
     2641 tactcgtcat cggattgccc ctcgtcgccg cggcgctcgt agccatagac ggcaatttca
     2701 aacgctcgat agatgagcaa atagccgaag aggctactat cttcgattcc cgcatcgacg
     2761 cgacgaccga gacgcttgcg cgcttctcga gctacatctt cacgagggag gtggacaatc
     2821 ccgaggtgcg ttcgctgatg aggcgggcct ggctcggcga cgaggggcag cgagcggcct
     2881 accgggcaga gctacgctcg cgtatgcttt cagcctacga cctgatgggc gagtaccatt
     2941 accgccagct ccactttcac ctgcccgacg gcacgagctt cctgcgcatg cacagccctg
     3001 aaaaattcgg cgactcgctc gtcggcatca gggccacggt cgcggcggtc aacgagcggc
     3061 tgtcgcccgt catagccttc gaggaaggcc gcatctaca
//
LOCUS       DAOOLN010000080         3069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_38742_length_3069_cov_2.745853, whole genome shotgun sequence.
ACCESSION   DAOOLN010000080 DAOOLN010000000
VERSION     DAOOLN010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38742_length_3069_cov_2.745853"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1067)
                     /locus_tag="PLW80_01610"
     CDS             complement(60..1067)
                     /locus_tag="PLW80_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPB65226.1"
                     /translation="MKKPRLRSIDDLAKLAGVSKSTVSRALNDSPLVGVATKDKILAL
                     AKDYDFRPSVTARALSTRSSRTVAFVIDAYDPECGLHSPFSLEIMGGAATGLHELGYG
                     MLIVQADGARGDWAEEYLVSGRVDGFILMTYENKRRHVQALVDLEAPFTVWGSCDMDP
                     CCSVSTDNRLGGRLAGEHLVATGRRRIGFIGGSMDDSEVVQRLLGFSEAVAIDRSLHT
                     PGLEAFGDFSEPSGFVAMNELLDRDGSVDAVFAAGDMMAIGAIRALATRGRRVPDDVA
                     VVGYDDLFVSAFTTPSLTTISQHIADTGRTLASRLVANIERGVVESAVVPVELIKRDS
                     A"
     gene            1166..2356
                     /locus_tag="PLW80_01615"
     CDS             1166..2356
                     /locus_tag="PLW80_01615"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPB65227.1"
                     /translation="MPNHSSLAAVMFVELAGFSDILAASERTALSLLGEYRRLADPIV
                     AEHSGETVDATNSELLVVFSSAVAAVQCALHLVLALAPLSAEGRDDVIAEPRVGLHLG
                     EIWRDSGRVYGNGVNVAARVMQAAPAGAVYLTEDVFRQVSSKLDLNSRPVGAVSMKNI
                     DRPIALYELDAGRGFVEGVAADGASAAGGDRPCEPETASNGPAESGGNLSDDAAAIAA
                     GIASLKQLKNLKSLKDLGSLKALEGLKGLKALEKLEALDRLDKDGELDGLGESIARRV
                     EANLKGIPGVTARTDDGTLDVSFEIGSGKVAGAAGRQAVDPGAAADKARARIAAGLRK
                     MLMSFGLGAAFGYGYASTDQWLFLAGAAAFGLFPFISGAKRLISAASELRLIEKRRKR
                     GAGA"
     gene            complement(2417..>3069)
                     /locus_tag="PLW80_01620"
     CDS             complement(2417..>3069)
                     /locus_tag="PLW80_01620"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPB65228.1"
                     /translation="QYMKKYLVLAGNIGAGKSTLVGLLAERLGYRPYFEPVAENPYLE
                     DFYADMERWAFHSQAFFLTHRVRSHRALMDDAYSVVQDRSMYEDAEVFARNLYERGSM
                     SARDWKSYSELYRTLTSLLPPPDLVVYLRASVPTLKRRIAKRGRDFEASISDEYLAGL
                     NRLYEEWIDGFDLAPVLVVPGDRLDFVAESKDLDSIVRTIEGRLRDKQGSLFPVGM"
BASE COUNT          551 a          950 c         1028 g          540 t
ORIGIN      
        1 cctccttctc attgtgcaac cggttgcaaa accgagaaaa aaaagatgca ggcatcagat
       61 caggccgaat cgcgtttgat cagctccact ggaacgacgg cgctctcgac gacgccacgc
      121 tcgatgttcg ccacgaggcg gcttgcgagg gtacgcccag tatctgctat atgctggctt
      181 atcgtcgtca gcgacggcgt cgtgaacgcc gagacgaaca ggtcgtcgta gccgacgacg
      241 gcgacgtcgt cgggcacgcg cctgcccctg gtcgccaggg cgcgtatcgc gccgatggcc
      301 atcatgtctc cggcggcgaa cacggcgtct accgaaccgt ctcggtcaag gagctcgttc
      361 atcgctacga agccggacgg ctctgaaaaa tcgccgaacg cctcgagccc gggcgtgtgg
      421 agggacctgt ctatggcgac ggcctcggag aagccgagga gccgctgcac cacctcgcta
      481 tcgtccatcg acccgccgat gaagccgatg cgacggcgac cggtggcgac gaggtgctcg
      541 cctgccaggc gtcccccgag gcggttgtcg gtcgagacgg agcagcacgg atccatgtca
      601 caggaacccc agacggtgaa tggggcctcg aggtcgacga gagcctgcac gtgtcgccgc
      661 ttattctcgt aggtcattag gatgaagccg tctactcggc ctgagaccag gtactcctcg
      721 gcccagtcgc ctcgcgcccc gtcagcctga acgatcaaca tgccgtaacc cagctcgtgg
      781 aggcccgtgg cggctcctcc catgatctcc aggctgaacg gcgagtggag gccgcattcg
      841 gggtcatagg cgtctatcac gaaggctacc gtacggctgg agcgcgtcga aagggcgcgg
      901 gcggtgacgc tcggtctaaa atcgtagtcc ttggcgagcg cgaggatctt atccttcgtg
      961 gcgacgccga cgagagggct gtcgttgagc gctctcgaga cagtggactt ggagacgcca
     1021 gcaagcttgg cgaggtcgtc tatcgaccta agtcgtggtt tcttcatatt gcaaccggtt
     1081 gcaatatatg attgccccga ctcatctgtc aagtggcgag cgatgtcact tgccaacaac
     1141 acgcccggct ctttacattt atgtgatgcc gaaccatagc tcgctggccg ccgtgatgtt
     1201 cgtcgaactt gccggtttct ccgatatcct ggccgcgagc gagcgcacgg ccctgtcgtt
     1261 gttgggcgag tatcggcgcc tagccgatcc catcgtcgcc gagcattcgg gcgagacagt
     1321 cgacgcgacg aattccgagc tactcgtcgt gttctcgagc gcggtggcgg cggtacagtg
     1381 cgccctgcac ctggttctgg cgctggcgcc gctgtcggcg gaaggtcggg atgatgtgat
     1441 cgccgagcct cgcgtcggcc tgcaccttgg agagatatgg cgcgattccg gccgcgtcta
     1501 tggaaatggc gtcaacgtgg ccgcccgcgt gatgcaggcc gccccggccg gcgccgtcta
     1561 cctgacggag gacgtgttcc gccaggtatc gagcaagctc gacttgaact cgaggcccgt
     1621 tggcgccgta tcgatgaaga atatcgatag gcctatagcg ctctacgagc tcgacgccgg
     1681 gcgcggcttc gtcgaaggcg ttgcggccga tggcgcgtcg gccgcaggcg gagatcggcc
     1741 atgcgaacca gaaacggctt cgaacgggcc cgccgaaagc ggtgggaacc tctccgacga
     1801 cgcggcggcg atcgccgccg gtatcgcgag cctcaaacag ctcaagaacc tcaagagcct
     1861 aaaggacctg ggctcgctca aagccttgga aggcctcaag ggactcaagg ctctggagaa
     1921 gctcgaggcg ctcgaccggc tcgacaagga cggcgagttg gacggcctcg gcgagagcat
     1981 agcccgtagg gtcgaagcca acctgaaagg gattcctggc gtgacggcca ggacggacga
     2041 cggtacgctg gacgtctcgt tcgagatcgg ttcagggaag gtcgccggcg cggccggtcg
     2101 ccaagccgtg gatcccgggg ccgcggccga caaggctagg gcgaggatag ccgccggcct
     2161 gcggaaaatg ctcatgagct tcggcctcgg agccgcgttc ggctacggct acgcgtcgac
     2221 cgaccagtgg ctgttcctgg cgggggcggc agccttcggg ctcttcccgt tcatctccgg
     2281 cgccaagcgc ctgatctcgg ccgcctcgga gctacgtctg atcgagaagc gccggaagcg
     2341 cggcgccggg gcctagcgct cggcgcgggg gcctagcgcg cggcgcgggg gcctagcgcg
     2401 cggcgtagga tgccggttac atgcctaccg ggaataggct gccctgcttg tcgcgcagtc
     2461 gaccttctat agtgcgcacg atcgaatcga ggtccttcga ctcggcgacg aagtccagcc
     2521 ggtcgcccgg cacgacgagc accggcgcga ggtcaaagcc gtctatccat tcctcgtaaa
     2581 gccggttgag cccagccagg tactcgtcgg atatgctcgc ctcgaagtcg cggccgcgct
     2641 tggctatgcg cctcttgagc gttggcaccg aggcgcgcag gtagacgacc aggtcagggg
     2701 gcggcaggag gctcgtcagc gtgcggtaca gctcgctgta gctcttccag tcgcgcgcgc
     2761 tcatcgagcc gcgctcgtac aggttgcggg cgaagacctc cgcgtcctca tacatggagc
     2821 ggtcctgtac caccgagtag gcgtcgtcca tgagggcgcg gtgggagcgt acgcgatggg
     2881 tcaggaagaa ggcctgggag tgaaaggccc agcgctccat gtcggcgtag aagtcctcca
     2941 ggtacggatt ctccgccacc ggctcgaaat agggccggta gccgagtcgc tccgcgagga
     3001 gcccaacgag agtggatttt cctgcgccta tgttgccggc aagaacgaga tatttcttca
     3061 tatactgtt
//
LOCUS       DAOOLN010000081         3067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_38773_length_3067_cov_3.740704, whole genome shotgun sequence.
ACCESSION   DAOOLN010000081 DAOOLN010000000
VERSION     DAOOLN010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38773_length_3067_cov_3.740704"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PLW80_01625"
     CDS             complement(<1..649)
                     /locus_tag="PLW80_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB65229.1"
                     /translation="MSGALTIAKRELSAYFNSPVAYIFIVAFLLSCGTLFFFVGDFFA
                     AGQATMRGYFSLMPFVMSVLVPALTMRLWAEERKQGTYELLLTMPLMDGQLVVGKYLA
                     ALAVVAAALALSLPVPLLASMFGRFDSGTIVAEYLGALLMASAASAIGQAVSSASKNQ
                     ISAFMATVMLLLALSLMSRLTTLLSLPGWLAGLVNWVSLSYHFGSFSRGVIDTRDV"
     gene            complement(646..1614)
                     /locus_tag="PLW80_01630"
     CDS             complement(646..1614)
                     /locus_tag="PLW80_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB65230.1"
                     /translation="MVEVSALVKRYGSFEAVRGVSFSVGSGKVLGLLGPNGAGKTTIM
                     KVLTGYHRPSSGRAVLDGRNVVEDPEGVKASLGYLPEGVPLYLDMTVAEYLDFVAAAR
                     GLGGKERRAARKAAIERAVDACGLSGVAGIRLERLSKGFRQRVGLAQAIVHDPAILIL
                     DEPTTGLDPNQILEIRSLIRSLGAEKTVILSTHILQEVEALCSEVIILNDGLVVAMGS
                     AEAIGAGLQGDERLECAVKGGFDPVEALTSIRAIPNVLSAEPTQAASGETTRFAVTAG
                     KGRGDDVAEGLFDWAAANGTKLIELKRERLSMEDIFVRLTREEPKR"
     gene            complement(1679..2608)
                     /locus_tag="PLW80_01635"
     CDS             complement(1679..2608)
                     /locus_tag="PLW80_01635"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002679668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HPB65231.1"
                     /translation="MNLEAFILGCGGMMPLPHRHLTSVLLRREGDLFLFDAGEGTQVA
                     LRRLNLRWKKISAIFISHTHADHVTGLPGLLMLSSQVDRDEPLYIYGPPRIAEYVETS
                     RKVLDMYINYEIVVKEVQAPAVCYEGDGFVVRAFPLRHTKTCYGYAMEESPRPGAFHP
                     ERATELGVQRGPLWSRLQAGEVVQASDGREVRPEDVLGPTRSGRKFSFVTDSLYLPEI
                     APEVAGSDLFVCEGMFERELLQSAVEKKHMAAFQAATIARDAGGVKKLALIHYSPRYA
                     DQELRVLQDEARAIFPETVLSKDRMRFSLDYVD"
     gene            2716..>3067
                     /locus_tag="PLW80_01640"
     CDS             2716..>3067
                     /locus_tag="PLW80_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPB65232.1"
                     /translation="MQAFMDLGLNDALAKAFVSLGFEDPTEIQKLAIPAILAGRDAYV
                     SSATGTGKTFAYLAPILDSLLSSAEDSPASVQAIVVAPTHDLAVQIEREAGRLAEAAG
                     FKLRAMCALGSVPLK"
BASE COUNT          567 a         1007 c          972 g          521 t
ORIGIN      
        1 ccacatccct cgtatcgatc acacctctcg agaacgagcc gaaatggtac gacagcgaca
       61 cccagttgac gaggccggcc agccagccgg gcagcgatag gagcgtggtg agcctggaca
      121 tgaggctgag cgccaggagc agcatcaccg tggccatgaa ggcgctgatc tggttcttcg
      181 acgcgctcga caccgcctgg cctatggccg aggcggccga ggccatcagg agggcgccca
      241 ggtactcggc gactatggtg cctgagtcga agcggccgaa catcgaggcc aggagcggca
      301 ccggcaggct cagcgcgagc gcggcggcca cgacggccag ggccgcgagg tacttgccga
      361 cgacgagctg tccgtccatc aatggcatgg tcaggagcag ctcataggta ccctgcttgc
      421 gttcctcggc ccagagcctc atcgtcagcg ccgggacgag gaccgacatg acgaacggca
      481 tcagcgagaa gtacccgcgc atcgtcgcct gcccggcggc gaagaaatcg ccgacgaaga
      541 agaacagcgt cccgcaagag agcaggaacg cgacgatgaa gatataggcc accggcgagt
      601 tgaaataggc cgaaagttcc ctcttggcta tagtaagggc gccgctcatc gctttggctc
      661 ctccctggtc agtcgcacga aaatatcctc catcgagagg cgctcgcgct tgagttcgat
      721 gagcttcgtg ccgttggcgg cggcccaatc gaacagcccc tcggcgacgt cgtctccccg
      781 gcccttgccg gcggtcacgg caaatcgggt cgtctcgccg ctcgcggctt gggtcggctc
      841 cgcggacagc acgttcggga tggcgcgtat agacgtcaga gcctcgacgg ggtcgaaacc
      901 gcccttgacg gcgcattcga gccgttcgtc gccctgcagg cccgcgccta tggcttcggc
      961 gctacccatg gcgacgacga gtccgtcgtt gaggatgatc acctcggagc agagcgcctc
     1021 gacctcctgg agtatatggg tcgacagtat gaccgtcttc tccgccccga gggaacggat
     1081 cagcgaacgg atctcgagga tctggttcgg gtcgaggccg gtcgtcggct cgtcgaggat
     1141 gagtatggcc ggatcatgca cgatggcctg ggccagcccg acgcgctgcc tgaagccctt
     1201 ggatagccgc tcgagccgaa tgcccgctac gcccgacagg ccgcaggcgt cgacggcccg
     1261 ctcgatcgcg gcttttctgg cggcgcgcct ctccttgcct ccgagcccgc gggcggcggc
     1321 gacgaagtcg agatactcgg ccacggtcat atcgaggtag aggggtacac cctcgggcag
     1381 gtagcctagg gaggccttca ctccctccgg atcctcgacg acgttccgtc catcgaggac
     1441 ggcgcgaccg gaactcggcc ggtgataccc ggtcagcacc ttcatgatcg tcgtcttgcc
     1501 agccccgttc gggccgagca ggccgagcac cttgcccgag ccgacggaga agctcacgcc
     1561 gcgcacggcc tcgaaggagc cgtagcgctt gacgagagcc gatacttcta ccatacgagc
     1621 ctccagaaca taatccggga ctgaaacaac ccggggttcg ggtggttacc gctccgcgtc
     1681 agtccacgta gtcgagcgag aagcgcatcc tatccttgga gagtacggtc tcggggaata
     1741 tcgcccgcgc ctcgtcctgc agtacgcgca gctcctgatc ggcgtagcgc gggctatagt
     1801 ggatcagggc cagcttcttg accccgcctg cgtcgcgggc gatggtcgcg gcctggaagg
     1861 ctgccatatg cttcttctcg acggcgctct gcaatagctc ccgctcgaac atgccctcgc
     1921 agacgaacag gtcggagccg gccacctcgg gcgcgatctc aggcaggtac agtgagtcgg
     1981 tgacgaagct gaatttcctg ccgctcctcg tcgggccgag cacgtcttcc ggcctgacct
     2041 cccggccgtc cgaggcctgc acgacctcgc cggcctgcag cctcgaccag agcggtccac
     2101 gctggacgcc cagctcggta gccctctccg ggtggaaagc gccgggacgc ggcgactctt
     2161 ccatggcgta gccgtagcag gtcttggtat gcctgagcgg gaaggccctg actacgaagc
     2221 cgtcgccctc gtagcagacg gccggcgcct gtacctcttt gaccacgatc tcatagttaa
     2281 tgtacatgtc gagcacctta cggctagtct cgacgtactc ggctatacgc ggcgggccgt
     2341 agatgtacag gggctcgtcc cggtcgacct ggctcgacag catgagcaag ccaggcaggc
     2401 cggtgacgtg gtcggcgtgg gtatgcgaga tgaagatggc gctgatcttc ttccagcgga
     2461 ggttaagccg ccggagggcc acctgcgtac cctccccggc atcgaagagg aacaggtcgc
     2521 cctcgcgcct gagaaggacg ctggtaaggt gccgatgcgg caggggcatc atgccgccgc
     2581 agccgaggat aaaggcttct aggttcatgt gggactacta tacgtaaaaa gcggcatacg
     2641 atgcaacgga gaagtcgtga cgtcacgctc gcccgactat tgcgtatcgc tccgctttta
     2701 tgcgaatata ttaagatgca agcatttatg gacctcgggc tgaacgatgc cttagccaag
     2761 gccttcgtct cccttggatt cgaagatccg actgaaatcc agaagctggc catcccggcc
     2821 atactcgccg gcagggacgc ctacgtcagc tccgcgaccg gtaccggcaa gaccttcgcg
     2881 tacctcgcgc cgatactcga ctccctccta tcctcggcgg aagactcccc ggcctcggtc
     2941 caggccatcg tcgtagcgcc aacccacgac ctggccgtcc agatcgaacg cgaggccggt
     3001 cgcctcgccg aggccgccgg cttcaagctg cgagccatgt gcgccctcgg ctcggtaccg
     3061 ctcaaac
//
LOCUS       DAOOLN010000082         3063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_38834_length_3063_cov_2.560173, whole genome shotgun sequence.
ACCESSION   DAOOLN010000082 DAOOLN010000000
VERSION     DAOOLN010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38834_length_3063_cov_2.560173"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="PLW80_01645"
     CDS             <1..727
                     /locus_tag="PLW80_01645"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPB65233.1"
                     /translation="VDTADTSGSWLAYVVGLARGRGTPLALYNAGPSRKTEAWLADAP
                     LFDDPDRTVAYFRGEAVAWAVKEGRRMAKAALLELGISWHAESLAQCVRDGDTKAVEL
                     FIASGFPPDVRDKAGVPMLCLAARSRHVGIVELLLDKGANIDAQSDDRGYSALMDAAQ
                     QGDDRLLEILLARHADPDLRSKDGQTALVLAVGRSDATAVERLLRGGADPDIADKLGL
                     SARKYAKLFHDPRIDEAFSAIAG"
     gene            784..854
                     /locus_tag="PLW80_01650"
     tRNA            784..854
                     /locus_tag="PLW80_01650"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:816..818,aa:Gln,seq:ctg)
     gene            894..965
                     /locus_tag="PLW80_01655"
     tRNA            894..965
                     /locus_tag="PLW80_01655"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:927..929,aa:Glu,seq:ctc)
     gene            1223..2191
                     /locus_tag="PLW80_01660"
     CDS             1223..2191
                     /locus_tag="PLW80_01660"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HPB65234.1"
                     /translation="MNEVPMASPVISLAPLQGVTTRVFRNAYAKRFGGVDYAIAPYLM
                     SVPTPDPRGDHFKELFPEYNARMPVVPQILSNDAAGFVATASALEALGYVEVNWNLGC
                     PYPMVTRKRRGAGLLPHPDVVRAFLEEACGRIRLELSIKLRLGLADSAEILGLMPILN
                     DYPLRSVILHPRVGAQLYEGSVDLDAFAAAASLSTRELAYNGDIVDEASYATIRARFP
                     SVSEFMLGRGVVSDPFLPARIKGLGLPDDPKATIKAFHDDVYFSYRETLSGRTHALDK
                     MKELWSYLGASFARSEDGLKEIRKAASFEAYEKAVRMAFSDDTAQQ"
     gene            complement(2235..2507)
                     /locus_tag="PLW80_01665"
     CDS             complement(2235..2507)
                     /locus_tag="PLW80_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPB65235.1"
                     /translation="MGRKIYVGNLSYNTYENALRDLFAAYGQVDSVKIITDRDSGQSK
                     GFCFVEMGSDAEAQAAIQGINGREVDGRQLKVNEAMDKPRRDNNRY"
     gene            2881..3030
                     /locus_tag="PLW80_01670"
     CDS             2881..3030
                     /locus_tag="PLW80_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65236.1"
                     /translation="MSLLNRRAGGATILVTRKRSTMPHTRNYRLLVLMALMQGIVFYG
                     PVATI"
BASE COUNT          565 a          942 c          968 g          588 t
ORIGIN      
        1 cgtcgatacg gccgatacga gcggatcctg gcttgcctat gtcgtcggcc ttgcccgcgg
       61 cagggggacg ccactagcct tatataacgc cgggccatcg cgcaaaaccg aggcctggct
      121 cgccgacgcg ccgctattcg acgatccgga ccggacggta gcgtatttca gaggcgaggc
      181 ggtggcatgg gccgtcaagg aggggcgccg gatggctaag gccgccctgc tcgagctggg
      241 catatcctgg cacgccgagt ccttggcgca gtgcgtccgc gatggcgaca cgaaggccgt
      301 cgagctattc atcgcctcgg gcttcccgcc cgacgtgcgc gacaaggccg gcgtcccgat
      361 gctctgcctg gcggccagat cgaggcatgt cggtatcgtc gagctgctcc tcgacaaagg
      421 cgcgaacata gacgcccaga gcgacgaccg gggctactcc gcgctgatgg acgcggcgca
      481 gcagggcgac gatagactgc tcgagatcct gctcgctagg cacgccgacc ccgacctccg
      541 tagcaaggac ggtcagaccg cgctcgtgct cgccgtgggg cgtagcgacg cgaccgcggt
      601 ggagcggctc ctgcgcggcg gagccgatcc cgacatcgcc gataagctag gcctcagcgc
      661 caggaagtac gcgaagctgt tccatgatcc gcgcatcgac gaggcgtttt cggccatcgc
      721 cggataagcg atactcttga ctctctgaat cgatttcagc tatattccac ctgcccaatg
      781 cattccccgg ttgtgtaatg gtagcacagc agactctgga tctgcttgtg ggggttcaaa
      841 tcctccctgg ggaactacct gtaatcggga tcaccgatcg cagcgaccga tacggcccct
      901 tcgtctatcg gttaggacaa aagattctca atctttaaag aggggttcga ctcccctagg
      961 ggctaaaaag acgacccagg caaagcctgg gtcgtctttt taattaagcc ccaggggagt
     1021 cgaaccggag gggccggtcc gagcgaacgc gaggacgagg cgcatggatg cgccgagagg
     1081 acccggaggc ggcccggagc gaggagccag gacggctccg aagcgtaggg cggctcccct
     1141 aggggctgca ctaggggagt cgaaggggtt atcgctctcc gggtctcccc acgttccgcc
     1201 gatacgagtt actatccttc gcatgaacga agtaccgatg gcgtcacccg ttatttccct
     1261 tgccccgctc cagggcgtga cgacgcgcgt gttcaggaac gcctacgcga agcggttcgg
     1321 cggcgtcgac tacgcgatag cgccgtatct catgtcggtg ccgacgcccg atccgcgcgg
     1381 cgaccacttc aaggagctat tcccggagta caacgcgcgc atgccggtcg tgccgcagat
     1441 cctgagcaac gatgcggcgg gcttcgtcgc gacggcctcg gcgctcgagg cgttaggcta
     1501 cgtcgaggtg aactggaacc taggctgccc gtacccgatg gtcacgcgta agcggcgcgg
     1561 ggcggggcta ttgccgcatc ccgacgtagt cagggccttc ctcgaggagg cttgcggtcg
     1621 tatacggctc gagctgtcga tcaagctacg gctcggcctc gcggactcgg cggagattct
     1681 cgggctcatg ccgatcctga acgattatcc gctacgctcg gtcatactgc acccacgggt
     1741 cggcgcgcag ctgtacgaag gctcggtaga cctggacgcc ttcgccgcgg ccgcgagcct
     1801 gtcgacgcgc gagctcgcgt ataacgggga tatcgtcgac gaggcctcgt acgcgacgat
     1861 acgcgcccgt ttcccttccg tgagcgagtt catgctgggg cggggcgtcg tatccgatcc
     1921 cttcctgccg gccaggatca agggcctagg cctgcccgac gatcccaagg cgacgatcaa
     1981 ggcgttccat gacgatgtgt atttcagcta cagggaaacg ctgtcgggac ggacgcacgc
     2041 gctcgacaag atgaaggagc tatggtcgta cctgggcgcc tcgttcgcgc gctcggagga
     2101 cgggcttaag gagatccgta aggcggcgag tttcgaagcc tacgagaagg cggttcggat
     2161 ggcgttttcc gacgacacgg cgcagcaata aaaaaaaccg gcctcgcggc cggttcctta
     2221 tccacggtta ctgattaata gcggttgttg tcgcggcgcg gcttatccat cgcctcgttc
     2281 accttgagct gcctgccgtc gacctcgcgg ccgttgatgc cctggatggc ggcctgcgcc
     2341 tcggcatccg agcccatctc gacgaagcag aagcccttgg actggccgga gtcgcgatcg
     2401 gtgatgatct ttaccgagtc gacctggccg taggcggcga agaggtcgcg gagagcgttc
     2461 tcgtaggtgt tgtagctcag gttgccaacg tagattttcc tgcccatggt tcgttccttt
     2521 tcggaggcct agcctccgag cgtttgatag tacgcctata cagatacagg cgattgcggg
     2581 agttcaggac caccgggcgt aaatctttgc gtcggccgag gatcgcgatc gctcgcgaac
     2641 cccgggaaag ggcgttacat cgatgtctag atagactctc tcggaaccta gtatatacga
     2701 agaatccgaa cgtacctagt accgtcaagg cggaattgcc ctcgtttgat cgaattccat
     2761 cggggtttta acggtttttc ctacccgcat gaaccgtcgg agccgtcctg gtcaataatc
     2821 ttgaccggac ccatgagccc ggttactcta ttgccatgcg accgatctga tcgtcagggg
     2881 atgagtcttc tcaatcgtcg cgccggaggc gcgaccatcc tcgttacacg aaagcgatca
     2941 accatgccgc atactcgcaa ctaccgcctg ctcgtgctga tggcgctgat gcagggcatc
     3001 gtgttctacg gtcccgtggc caccatctag ccgcgcctac ggcctcgacg tcgaggccct
     3061 ctt
//
LOCUS       DAOOLN010000083         3060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_38886_length_3060_cov_2.891847, whole genome shotgun sequence.
ACCESSION   DAOOLN010000083 DAOOLN010000000
VERSION     DAOOLN010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38886_length_3060_cov_2.891847"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLW80_01675"
     CDS             complement(<1..385)
                     /locus_tag="PLW80_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65237.1"
                     /translation="MTLLSMALRNLGRNRRRTALAASSVAIAILLVVFMQGMVGGFLG
                     SIVKNYTKNEVGHVNIVTEEYRERERFSPVDAAIPDSSAVIRALEGAPGLEGAKFAER
                     IRFGVILSAGANSKAALGLAGDPVTE"
     gene            complement(396..1655)
                     /locus_tag="PLW80_01680"
     CDS             complement(396..1655)
                     /locus_tag="PLW80_01680"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPB65238.1"
                     /translation="MGTLWKIAARNVLRHKRRTVITGVVMAVGIGLFIMMDSVLVGMD
                     RITLDNMIDYTESVAKVRTPEYLSNQLGTPLDYGVPDPEAAMRAIMAADGRVTAVAPR
                     ARFVANVSNYEDSLPVVATAVDPELDALVFRLPEAVVSGSWLSREAPGDVVIGSGLAK
                     ELGVEVGDYLVVSASTVYENVNADEYRVGGVVFTPMPYINDSGLFMAYGDADALLGTD
                     GFASELVVASARAASLSATLGNADDIAVQAAAALPGLRVDSITELAKDYLAMRAMKSK
                     FSYMIIVVVLLIAAVGIVNTILMSVYARIREIGVLRAYGMSPKDIRRLFTLEGLLVGA
                     VGSLGGVLIGALMDWYFVKVGMSIGAIVGSIDMGGLPLTGTLYGEWNPATMAVGFAFG
                     LVVAFLAARIPAKRAARLEVTDALRFV"
     gene            complement(1658..1942)
                     /locus_tag="PLW80_01685"
                     /pseudo
     CDS             complement(1658..1942)
                     /locus_tag="PLW80_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(1879..2238)
                     /locus_tag="PLW80_01690"
     CDS             complement(1879..2238)
                     /locus_tag="PLW80_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB65239.1"
                     /translation="MAVVGPSGSGKTTLLNIIGCLDKPSSGRVVYEGADLGSATQQAL
                     SDYRKDRISFIFQSYNLIPVLTVAENVELPLVIEKRRDKAAMRAKALEMLAAVSLEGK
                     ANRFPRELSGARNSASL"
     gene            complement(2340..2678)
                     /locus_tag="PLW80_01695"
     CDS             complement(2340..2678)
                     /locus_tag="PLW80_01695"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HPB65240.1"
                     /translation="MAERSRLARLGLDKLIHEKSRLMILTYLASGGEPEIGFTELRDA
                     LSMSAGNLSIQLRTLEEAGLVSINKRFKANKSYTGVRLSVEGGRALDEYLSELETILA
                     TARKTRGEAL"
     gene            complement(2681..>3060)
                     /locus_tag="PLW80_01700"
     CDS             complement(2681..>3060)
                     /locus_tag="PLW80_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65241.1"
                     /translation="RMAHATMPLTLGMVAGIAYAFYAGRPWLALPVSSFLFGVMANVI
                     AGKGGLRAYYVIGYWGILLGLAALPLVERAPFLWLFVIYGGMFFAFAITQAADNAVAR
                     GAGQDSRQTTDRSDAPDGREAGD"
BASE COUNT          503 a         1033 c          977 g          547 t
ORIGIN      
        1 tctcggttac cgggtcgcct gccagcccga gcgcggcctt gctattggcg ccggcggaca
       61 gtatcacccc gaagcgtatc cgctcggcga acttggcgcc ctcgaggcca ggtgcgccct
      121 cgagcgcccg tatgacggcg ctcgaatccg ggatggccgc gtcgaccggc gagaagcgct
      181 cccgttccct atattcttcg gtcacgatat tcacgtggcc gacttcgttc ttggtgtaat
      241 tcttgacgat cgacccgagg aagccgccga ccatgccttg catgaagacg acgagcagga
      301 tggctatggc gacggacgac gccgcgagcg ccgtacgcct gcggttcctg cccaggttcc
      361 gtagcgccat cgaaagtagt gtcatcgcgt cctccttaga cgaagcgcag cgcgtcggta
      421 acttccagcc tggcggcgcg cttggccggt atcctcgcgg ccaggaacgc cacgaccagc
      481 ccgaacgcga agccgaccgc catggtcgcc gggttccact cgccgtagag cgtgccggtc
      541 agcggcaggc cgcccatgtc tatcgacccg actatcgcgc ctatcgacat gccgaccttg
      601 acgaagtacc aatccatcag ggcgccgatt agcacgccgc cgaggctgcc gacggcgccg
      661 accagcagac cctcgagcgt gaagagcctc cgtatgtcct tgggcgacat gccataggcc
      721 ctgagcacgc cgatctcgcg gatcctcgcg tagacgctca tcaggatggt gttgactatg
      781 ccgactgccg ctatgagcag cacgacgacg atgatcatgt acgagaactt gctcttcatc
      841 gcgcgcatgg ccaggtagtc cttggccagc tcggtgatcg agtcgacccg caggccgggg
      901 agcgcggcag cggcctgcac ggctatatcg tcggcgttgc cgagggtggc gctcagactg
      961 gccgctctcg cgctggccac gaccagttcg ctcgcgaagc cgtcggtgcc gagcagcgcg
     1021 tccgcgtcgc cgtaggccat gaataggccg gagtcgttga tatagggcat cggggtgaag
     1081 acgacgccgc ctacgcggta ctcgtcggcg ttcacgttct cgtagaccgt cgaggctgaa
     1141 acgacgaggt agtcgccgac ctcgactccg agctccttgg ccaggccgga gccgatgacg
     1201 acgtccccag gcgcctcgcg gctcagccac gatcccgaga cgacggcctc gggcagcctg
     1261 aacacgagtg cgtcgagctc cgggtcgacg gccgtggcga cgaccggcag cgagtcctcg
     1321 tagttcgaga cgttcgccac gaagcgggcg cggggcgcga ccgccgtgac tcggccgtcc
     1381 gcggccatga tggcccgcat cgccgcctcg gggtcgggga cgccgtagtc cagcggcgtg
     1441 ccgagctggt tcgagaggta ctccggcgtg cggaccttcg ccaccgattc cgtatagtct
     1501 atcatattgt ccagggtgat gcggtccata ccgacgagca ccgagtccat catgatgaac
     1561 aggccgatac cgaccgccat gacgacgccg gtgatcaccg tacggcgttt gtgcctgagc
     1621 acgttgcgcg ccgctatctt ccagagcgtt cccacggcta cctcctctcg tcggcgacaa
     1681 gtcggccgtc tcggatggtc acgactctgc gggcgtgctg catgacgagc ttgtcgtggg
     1741 tcgagaagac gaaggtcgtc ccgcgcgagg cgttgatcgc cttcatgagg ttgacgatct
     1801 cctcggcggt atggctgtcc aggttggcgg tcggttcgtc ggcgagcacc acggacggat
     1861 ccttgaccag ggcgcgggct atagcgacgc gctgttcctg gcccccgaca gctcgcgcgg
     1921 gaagcggttc gccttgccct cgagacttac ggccgctagc atctcgagcg ccttggcccg
     1981 catggccgcc ttgtcgcgtc gcttctctat cacgagcggc agctcgacgt tctcggccac
     2041 ggtgagcacc ggtatcaggt tgtacgactg gaagatgaag ctgatgcggt ccttgcggta
     2101 gtcggatagc gcctgctgcg tcgcggaacc caggtcggcc ccctcgtaga cgacgcgtcc
     2161 cgagctgggc ttgtccaggc aaccgatgat gttgagcaac gtcgtcttgc ctgatcccga
     2221 cgggccgacc actgccataa actcccccgg ctcgacctcc atcgagacgc cgtcgagcgc
     2281 gcgcacctcg ttatcgccct tgctgtagac cttggtcagg tccttgagct ccagtacgtt
     2341 cataacgcct ccccgcgcgt cttgcgcgcc gtagccagaa tggtctcgag ttcggatagg
     2401 tattcgtcga gcgccctccc gccttccacg ctcaggcgga cgccggtata cgatttgtta
     2461 gccttgaagc gcttgttgat ggagacgagc cccgcctcct cgagcgtccg gagctggatg
     2521 gacaggttcc ccgcgctcat ggacagcgcg tcgcgtagct cggtgaaccc tatctccggt
     2581 tccccgccgg aagccaggta ggtcaggatc atcagccgcg acttctcgtg gatcagcttg
     2641 tccagcccga gcctcgcgag acggcttcgc tcggccacgg ctagtctccg gcctcgcggc
     2701 cgtcaggcgc gtcggaacgg tcggtcgttt gcctcgagtc ctgtcccgcg cccctcgcga
     2761 cggcgttgtc cgccgcctgg gtgatcgcga acgcgaagaa catgccgccg tagatgacga
     2821 acagccacag gaacggcgcc cgctcgacta ggggcagggc cgcgaggccg agcaggatac
     2881 cccagtagcc gattacgtag tacgccctga gtccaccctt cccggctatc acgttggcca
     2941 tcaccccgaa caggaaggag ctgaccggca gggccagcca gggcctaccc gcgtagaacg
     3001 cgtaggctat gccggccacc atgccgagcg tcagcggcat cgtagcgtgc gccattcgcc
//
LOCUS       DAOOLN010000084         3052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_39022_length_3052_cov_2.080747, whole genome shotgun sequence.
ACCESSION   DAOOLN010000084 DAOOLN010000000
VERSION     DAOOLN010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39022_length_3052_cov_2.080747"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PLW80_01705"
     CDS             <1..475
                     /locus_tag="PLW80_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65242.1"
                     /translation="GGAAMNCRSFRNQLDALDNDDLPIEMLVHARSCPECAAEAAALK
                     AALSLYRLPDLVGSPDVAPRVMALLPFVPAPRRSVSMGNWLVTGFVIVFSMVLVPLLA
                     EFRALKAVYGNVFTVSLSMALGLFVTLYAGAFIMSHLDEFTRRLKEREAQHGRAA"
     gene            complement(472..2442)
                     /locus_tag="PLW80_01710"
     CDS             complement(472..2442)
                     /locus_tag="PLW80_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65243.1"
                     /translation="MRTKPFILVGLYCIALFISCDFKTTIFGDFNSPNDPRTTEYADY
                     RIEVLAGIGSNPSPETTDATTLRLSHVTDLEASGGYLYVADSLHHRVIRIHETSGAAS
                     AIAGTGVPGYSGDGGPAASARLFAPTAVLAWDDGSLFILDAMNDAVRRVSADGVISTY
                     ADLAALTGEKADLWMQADWNRGCMARLQNDILVYHYHRIYRLSGTIGSVSAVEYPATD
                     FFGTIGGDPNSFGVDDGGALWLSAWLHESQKRVLGRVASGSTEFEVMREFDYGASDSI
                     DFDSSGKVLAYKDQDEIIAYDPSDGSVTNRTDEDGGSVFCINSSGVLYRYNVWCDLGQ
                     HVITKMTAPLTAPAVFAGIASLVPTGDPTDRIQPWHLALDEEDGELYYTDTSQTIVRS
                     VNVSTGQGTLVASGLPGSPKGVSIGPGGEIVFSNNGDSNIYRLSGSSYSLIATYDSPL
                     EDVAVAPNGAVFAASFTFSPSGGFTYFTGLVKRVSGEHSILRVGNGTPIAYADPDGSY
                     STAIPIGAVWAICFGPDGTLYLAEEERRILAVAPTGVVTRISGGIWTETSPDRVWFDS
                     FEIEDEFMKAREASIRGSPRQIAYGADGCLYIAHQYDAVLRVNAESRIEQLRFHLDGR
                     TVDLCARGIAVDSARRLYLATEYQIIRATPRL"
     gene            complement(2439..>3052)
                     /locus_tag="PLW80_01715"
     CDS             complement(2439..>3052)
                     /locus_tag="PLW80_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65244.1"
                     /translation="KYLWGSSDEVVARITKEGEDDSLPISLEQTNLFSAGRYVLEAKL
                     GNDWEWMFKQEIEIVPGSEQSLSIPHLDFSNSWKISDLTIQRSLAFDRLVAIQKARKS
                     RAAISNLGAVIGLAGLGVAVYGIVDGTSAYPEYQVAATPAEALLLRQRLVLDQNYLVF
                     GLAGSGASFLLWTVRFGKQEELRNAQDEIDRLERMIDELRSQE"
BASE COUNT          553 a          955 c          905 g          639 t
ORIGIN      
        1 aggaggggcc gctatgaatt gcaggagctt caggaaccag ctcgacgcgc tcgataacga
       61 tgacctgccg atcgagatgc tcgtccacgc gcgttcgtgt cccgaatgcg cggccgaggc
      121 cgccgccctc aaggcggcgc tgagcctgta ccgcctgccc gacctggtcg gctcccccga
      181 tgtcgcgccc agggtcatgg ccctcttgcc cttcgtgccg gccccgcgcc gctcggtgtc
      241 catggggaac tggctggtga caggcttcgt catcgttttc tcgatggtgc tcgtgcctct
      301 cctcgccgag ttccgtgcgc tcaaggccgt ctacggcaac gtcttcaccg tgtctttgtc
      361 gatggcgctc ggcctcttcg tcaccctcta tgccggcgcc ttcatcatga gccacctgga
      421 cgagttcacc cggcgcctca aggagcgcga ggcccagcac ggacgggctg cctagaggcg
      481 cggggtagcc ctgattatct gatattccgt agccaggtac aggcgcctcg ccgagtcgac
      541 ggctatcccc ctggcgcata ggtcgaccgt ccgtccgtcc aaatggaagc gcagctgctc
      601 tatccggctc tcggcgttga cgcggagcac cgcgtcgtac tggtgcgcta tatagaggca
      661 cccgtcagcg ccatacgcga tctgcctcgg cgagccgcgt atcgaggctt ccctggcctt
      721 catgaattca tcctcgatct cgaacgagtc gaaccagacg cgatcgggac tggtctcggt
      781 ccagatgccg cctgaaatgc gtgtaaccac gccagtgggc gcgacggcca gaatccgccg
      841 ttcttcctct gcgaggtaga gcgtcccatc gggcccgaag catatcgccc ataccgcccc
      901 gatgggtata gccgtggaat agctaccgtc agggtcggca tacgctatcg gcgtgccgtt
      961 ccccacccgg agtattgagt gctccccgga cacgcgtttg accaggcctg taaagtaggt
     1021 gaatcctccg gacggtgaaa aggtgaacga ggcggcgaag accgccccgt tcggcgctac
     1081 ggccacgtcc tcgagcggac tatcgtacgt ggcgatcaac gagtagctac tccccgaaag
     1141 cctatagata ttcgagtcac cgttattcga gaagactatt tccccacccg ggccgatgga
     1201 gacccccttg ggcgatcccg gaaggccgga agcgacgagg gtcccctggc ccgtcgagac
     1261 gttaacgctg cgtactatgg tctggctagt atccgtataa tagagttccc catcctcttc
     1321 gtccaaggcc aggtgccagg gttggattct gtccgtcggg tcgccggtgg gaaccaacga
     1381 agcgatcccg gcgaatacgg ctggggcggt caggggggcg gtcatcttgg ttatgacgtg
     1441 ttggcctaga tcgcaccaaa cgttgtagcg gtatagcacg ccggagctat tgatacagaa
     1501 tacagagcca ccgtcttcgt cggtacggtt ggtcacggag ccgtccgatg gatcgtaggc
     1561 gataatctcg tcttgatctt tgtaggcgag aaccttgccc gatgaatcga agtctattga
     1621 atccgatgct ccatagtcga attcgcgcat gacctcgaat tcggtcgagc cgctcgctac
     1681 tctgcccaag acccgcttct ggctctcatg taaccaggcc gatagccata gcgctccgcc
     1741 gtcgtcgacg ccgaacgaat tcgggtcacc cccgatggtg ccgaagaaat cggtggcggg
     1801 gtactcgacc gcgctgaccg aaccgatcgt gccgcttaac cggtagatgc gatggtagtg
     1861 atacaccagg atgtcgttct gcagccgggc catgcagccc ctgttccagt cagcctgcat
     1921 ccaaaggtcc gctttctcgc ccgtcagcgc ggccaggtcg gcgtaggtgc tgatgacgcc
     1981 gtccgcgctt accctcctga cggcgtcgtt catggcgtcc agtatgaaca gcgatccgtc
     2041 gtcccaggcc agcacggccg tcggagcgaa gagcctggcc gaggcggccg ggcctccgtc
     2101 gccggaatat cccgggacgc cggtccccgc tatcgccgat gccgcgccgc tcgtctcgtg
     2161 tatcctaatc acccggtgat gcaacgagtc ggcgacgtac agatagccgc ccgacgcttc
     2221 caggtcggtg acgtgggaca ggcgcaacgt cgtcgcgtcg gtcgtctccg gagaagggtt
     2281 cgagcctatg ccggctagta cctcgatcct gtagtcggcg tactcggtgg tgcgcgggtc
     2341 gttcggactg ttgaagtcgc caaagatggt ggtcttaaaa tcgcaggata taaagagcgc
     2401 gatgcaatac aggccgacga gtatgaacgg cttagttctc attcctggct cctcagctca
     2461 tcgatcatcc gctctaggcg gtcgatctca tcctgcgcgt tgcgcaattc ctcctgtttt
     2521 ccgaagcgta ccgtccagag caggaacgag gctccgctac ccgccagccc gaataccagg
     2581 taattctgat cgaggacgag tcgttgcctg agcagcaagg cttcggccgg ggtggcggcg
     2641 acttggtact cggggtaggc gctcgtgccg tccacgatac cgtagaccgc tacgcctaag
     2701 ccggctaaac ctataaccgc gccaaggtta gaaatcgccg ccctggactt cctggctttc
     2761 tggatggcta cgaggcgatc gaacgccagg ctgcgttgga ttgttaaatc cgatatcttc
     2821 catgagttcg agaagtccag atgcggtatc gatagcgatt gctcgctccc gggtacgatt
     2881 tcgatttcct gcttgaacat ccactcccag tcattgccga gctttgcctc taggacgtat
     2941 cggccggctg agaatagatt ggtttgctcc aagcttatcg gaaggctatc atcctctccc
     3001 tcttttgtta tccgcgctac gacttcgtcg ctggatcccc ataggtactt ga
//
LOCUS       DAOOLN010000085         3038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_39253_length_3038_cov_3.701307, whole genome shotgun sequence.
ACCESSION   DAOOLN010000085 DAOOLN010000000
VERSION     DAOOLN010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39253_length_3038_cov_3.701307"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="PLW80_01720"
     CDS             complement(<1..524)
                     /locus_tag="PLW80_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB65245.1"
                     /translation="MIEIRNASKSFGPTMAVDNVSLTIRDGELFGLLGPNGAGKSTLI
                     RMITGLLAQDGGSVSFFDGPAGTAGPLDAKAVKRMIGLVPQDLAFYQNYSAIENVKFF
                     ASLYGLRGKALTEAAEAALAFAGLSDTGKKPAKAFSGGMKRRLNIACGIAHDPRVVIL
                     DEPTVGIDPQSRNH"
     gene            complement(620..1249)
                     /locus_tag="PLW80_01725"
     CDS             complement(620..1249)
                     /locus_tag="PLW80_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB65246.1"
                     /translation="MAIRLLIGDDDPLIREALEIVFGRDEGFTVVASVADGLEAVRAC
                     RSLSVDVALLDIRMPGKNGIEAAAEIAVGGTCRVLLLSTFKETELVRGALASGASGYL
                     LKGCSSDEIKEAVRLVHQGHTVFQDEVFEAVRRASANAPGDLSALSERERSLVRLIAE
                     GYSNRQAADSLGLSEGTIKNYVSSILDKLGLQQRTQIAIYCVTGRRDFS"
     gene            complement(1221..2405)
                     /locus_tag="PLW80_01730"
     CDS             complement(1221..2405)
                     /locus_tag="PLW80_01730"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPB65247.1"
                     /translation="MPALEAIIAAVILVRYAIGRPDAIGPALAVFLAFQTLVAAGPWP
                     RPGTARQAAPRSAVWLVLAAAKLALCATAYRAEPACVLAWPAVIMGAPLRGPLAAPAD
                     DGWRPVPWVVAAALAAAPSPLIEVGQTPVYLGVWAVACAWAADSAVRSIRERRLRSRV
                     GSLERELASSQERLDGLRRVGAEAERLAAISERERLARSLHDELGHTLTGGIMQLDAA
                     RLLFDDEPERARRIVDRVSATLKDGLSSIRLSLRAMKPEAEAIGAQRLRAALEDFRSR
                     HGLETRLSLDGPLEAMPAEQWRVAADNLTEAMTNTLRHGRASLFSCSVTALNRLYKVE
                     FKDDGSTGAVVRQGMGLEGMETRTREAGGTMLVDSSRGFSVIMLFPRGGTNDGDTATD
                     RR"
     gene            complement(2466..2951)
                     /locus_tag="PLW80_01735"
     CDS             complement(2466..2951)
                     /locus_tag="PLW80_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65248.1"
                     /translation="MASGLALYAVHWFYEIVNALIQHFSGHALWTVPTGTSFLLLVGV
                     GWELSMMFAVAGLIFSKILPDDPKAKVLGVNNRVFFAVANAAFFSVFEIFLASTPAFH
                     WVYPWWGAFPVFITTYVPFFLAANLAYDWKPATRRRLIGGLFIVDAAMLAVFAGVLGW
                     I"
BASE COUNT          518 a          982 c         1054 g          484 t
ORIGIN      
        1 atatggttgc gcgactgggg gtcgataccg accgtcggct cgtccaggat gacgacgcgc
       61 gggtcgtggg ctatgccgca ggcgatattc aacctgcgct tcatgccgcc ggagaatgct
      121 ttggccggtt tcttgcccgt gtcggagagg ccagcgaacg ccagcgccgc ctcggcggcc
      181 tcggtcaggg ccttgccgcg gaggccgtac aggctggcga agaacttgac gttctcgatc
      241 gcgctatagt tctggtagaa ggccaggtcc tggggtacga gccctatcat gcgcttgacg
      301 gccttcgcgt cgagcgggcc ggccgttccc gccgggccgt cgaagaacga taccgagccg
      361 ccgtcctgtg ccaggaggcc ggtgatcatg cggatgaggg tcgacttgcc ggcgccgttg
      421 gggccgagca gtccgaatag ctcgccatcg cgtatggtca gcgagacgtt gtccacggcc
      481 atggtaggcc caaagctctt gctcgcgttc cgaatctcta tcatctcgtc ctcctcgccc
      541 tatcgtggtt cgagcatagc gacggcggag cgcggcgggt agtacgtttg gtaacgcgcc
      601 ggagtgacta aagtcaggcc taggagaagt ccctgcggcc ggtcacgcag tagatggcta
      661 tctgggtgcg ctgctggagc ccgagcttgt ccaggatgga cgagacgtag ttcttgatcg
      721 taccctcgga caggccgagg ctgtcggccg cctgccggtt cgaatagccc tccgctatca
      781 gcctgacgag ggagcgctcc cgctcgctca aggcggatag gtcgcctggc gcgttggccg
      841 aggcccgcct gacggcctcg aacacttcgt cctggaagac cgtgtgcccc tgatggacga
      901 gcctgaccgc ttccttgatc tcgtcgctcg agcagccttt gagtagatag cccgacgcgc
      961 cgctcgccag tgcgccgcgc acgagctcgg tctccttgaa ggtggacagg agtaggaccc
     1021 tgcacgtgcc accgaccgcg atctcggccg ccgcttcaat gccgttcttc ccgggcatgc
     1081 gaatgtcgag gagggccacg tcgacggaca gcgagcggca ggcgcgcaca gcctcgagcc
     1141 cgtcggccac ggaggcgacc acggtaaagc cttcatcccg cccgaagact atctcgagcg
     1201 cctcgcggat cagcgggtcg tcatcgccta tcagtagccg tatcgccatc gttcgtccct
     1261 cctcgcggga agagcatgat aaccgagaat cccctcgacg agtccaccag catcgtaccg
     1321 ccggcctcgc gggttctggt ctccatgcct tcgagcccca tgccctgccg gactaccgcg
     1381 ccggtcgagc cgtcgtcctt gaactcgacc ttgtagagcc ggttgagcgc cgtcaccgag
     1441 caggagaaga gactggcccg tccgtgccta agcgtgttgg tcatagcctc ggtcaggttg
     1501 tcggcggcga cgcgccattg ctcggcgggc atggcctcga gcggtccgtc gagggagagc
     1561 ctggtctcga ggccgtggcg ggagcggaag tcctcgagcg cggcccggag ccgctgggcg
     1621 ccgatcgcct cggcctcggg cttcatcgcg cgcaggctca ggcgtatcga cgataggccg
     1681 tccttgaggg tggccgaaac ccggtctacg atgcgcctgg cccgttcggg ctcgtcgtcg
     1741 aacaggagcc tggccgcgtc cagttgcatg atgccgccgg tgagcgtatg acccagctcg
     1801 tcgtggaggc tgcgcgccag cctctcgcgc tcgctgatgg cggcgaggcg ctcggcctca
     1861 gcgccgacgc gcctaaggcc gtcgagccgc tcctgggaac tggccagctc gcgctcgagc
     1921 gagccgaccc ggctacgcag gcggcgctcg cgtatcgaac ggaccgccga gtccgcggcc
     1981 caagcgcagg cgacagccca gacgccgagg tagaccggcg tctggcccac ctcgatcagc
     2041 ggggacggcg ccgcggcgag cgccgcggct acgacccatg gaacggggcg ccaaccgtcg
     2101 tcagccggcg cggctagcgg accgcgaagc ggcgcgccca tgatgacggc tggccaggcc
     2161 aggacgcaag ccggctcggc gcgataggcg gtagcgcata gagccagctt agcggccgcc
     2221 aggacgagcc agacggcgga gcgcggggct gcctggcgcg ccgtaccggg cctcggccac
     2281 ggtccggcgg ctaccagcgt ctggaacgcc aggaagacgg cgagggcggg cccgatcgcg
     2341 tcggggcggc cgatagcgta ccggacgaga atgaccgccg cgatgatagc ctccagggcc
     2401 ggcacgtcag cctccgtggg cgcggcggat cctcgtcggt agcggcccta tagtcgaccc
     2461 ttcggctaaa tccatccgag tacgccggcg aatacggcga gcatcgccgc gtcgacgatg
     2521 aacagcccgc ctatcagccg ccgcctggtc gcgggcttcc agtcgtaggc gagattggca
     2581 gcgaggaaga agggcacgta ggtggtgatg aacaccggga aggctcccca ccaggggtag
     2641 acccagtgaa aggccggggt ggaggcgagg aagatttcga agaccgagaa gaaggccgcg
     2701 ttcgccacgg cgaagaacac gcggttgttg acgccgagca ccttggcctt cgggtcgtcg
     2761 ggaaggatct tcgagaagat caggcccgcg acggcgaaca tcatcgacag ctcccagccg
     2821 actccgacga gcagcaggaa cgaggtgcca gtcggtaccg tccagagcgc gtggcccgag
     2881 aagtgctgga tgagggcgtt gacgatctcg tagaaccaat ggaccgcgta cagcgctagt
     2941 cccgaggcca ctccacgcca gtttttcttg aatctcgttg aagtagacgt acgcgacgaa
     3001 ggcgaggatg gggatgaaat accactggaa ggttgctg
//
LOCUS       DAOOLN010000086         3034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_39335_length_3034_cov_3.449144, whole genome shotgun sequence.
ACCESSION   DAOOLN010000086 DAOOLN010000000
VERSION     DAOOLN010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39335_length_3034_cov_3.449144"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PLW80_01740"
     CDS             complement(<1..502)
                     /locus_tag="PLW80_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPB65249.1"
                     /translation="MVSQIYGRLDDDDEEEDKTQRGPDVLAERLLETRTVLLSGQVNK
                     ELAERVVRQLLLLEAKGPEPIKIMIDSPGGDVDAGYAIFDMARFVKPEVWMIGMGLVA
                     SAGALILLAAPKKRRIAFPNSHYLIHQPLSGLRGVATEIEIHAREIEKTRDRINKLIA
                     AETGQPL"
     gene            729..1361
                     /locus_tag="PLW80_01745"
     CDS             729..1361
                     /locus_tag="PLW80_01745"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPB65250.1"
                     /translation="MISMPRTVIAASLALLSLASCSGPKDGEAPVSTMASAAPSSAET
                     PSILAADAAPWYAEPMEALGFFVFPRPEPMPPFEVAPLAGGRAGLGDAAGKVVLLNFW
                     ATWCPPCRSEMPSIQRLHDAMAGRPFAVMAVSVAESAATVRAFLKTDPYTFPIYLDEG
                     GRASGPFVGRGIPTTFVLDKQGRAIAGVIGARSYDDPEVLALFKALAERL"
     gene            1358..2299
                     /locus_tag="PLW80_01750"
     CDS             1358..2299
                     /locus_tag="PLW80_01750"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPB65251.1"
                     /translation="MIADVSILVALGAGLLSFLSPCVLPLIPGYLSFISGAGIERIRD
                     GSERSGVFWRTLFFVTGFSAVFIALGLAFSGGGMLTAGRANRVVTVAAGSLIAALGLN
                     MIFGFLRFLNLEARVKAPAKPRSAAGAFVVGMAFGAGWTPCVGPILASILVMAARSGG
                     AGRAVMLLTAYSAGLALPFIAAGLFFERLSPVWAWFKRHGRGVQIVSGLLLLAIGVSM
                     AIGRLGSIGAISARAGIGLKAALATDPAAVRLWSAAVVAALAAILVVAPLARRRPFAK
                     LWRIVALALLAVLLTLEAAGAVSMASLIADWLLFQGA"
     gene            complement(2296..>3034)
                     /locus_tag="PLW80_01755"
     CDS             complement(2296..>3034)
                     /locus_tag="PLW80_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HPB65252.1"
                     /translation="VSSMLLNVYSAVAVIETLPLTMREITILAVLCLSAHNLIIETAV
                     MRKSGSSAIKMLSLRILGGVALAYALNLLLPGDASSVAASTADTARAALLPALGAWGL
                     STLRLVVKILLLVNGIMIAQRLMEAFRVLDFLSRLAAPLMRVFGLSDNSSFLWIVINV
                     VGYAYGAAIIMERVKGGAMKPQEADLFNHHACLCHSLLEDTSLFLAIGTPLGWITLPR
                     IVAALLVVWLERLRRGRFRRSFRAGTA"
BASE COUNT          459 a          999 c         1013 g          563 t
ORIGIN      
        1 cgagcggctg gccggtctcc gcggctatca gcttgttgat gcggtcgcgg gtcttctcga
       61 tctcgcgggc gtggatctct atctcggtgg ccacgccgcg cagtcccgac agcggctggt
      121 ggatcaggta gtgcgagttc gggaacgcga tgcggcgctt cttgggggcc gctagcagga
      181 tgagggcgcc ggcgctcgcg acgaggccca tgcctatcat ccagacctcc ggcttgacga
      241 agcgggccat gtcgaagatg gcgtagccgg catcgacgtc tccgccgggg gagtctatca
      301 tgatcttgat cggctcgggg cccttggcct cgagcaatag cagctggcgg acgacccgct
      361 cggccagttc cttgttcacc tgtccggaca gcaggacggt acgcgtctcg agtaggcgct
      421 cggcgaggac gtccgggccc cgctgagtct tgtcctcctc ctcgtcgtcg tcgtccaggc
      481 gcccatatat ctgactgacc atgctcatgt cgtctcctcg aattcggtgt tggtaaaggc
      541 tcgcgcggag gcgaaccaaa agctagtata ataaaaaagt gccgattcgc ctatgcggcg
      601 gtccgaggcg gcgcttgctg gaatgcttga aatatagtaa attacctata taattcccaa
      661 tccgccatgc gctaccttga cgcatcaggg ttgcgaaccc gtcgtatagc gggccaatag
      721 gagacgcgat gatatcgatg ccccggacag tcatagccgc ttcgctcgcc ctgcttagcc
      781 tcgcctcgtg ctcggggccg aaggacggag aagcccccgt atcgacgatg gcctccgcgg
      841 cgccctcctc cgccgaaacg ccctcgatat tggcggcgga cgccgcgccg tggtacgccg
      901 agcctatgga ggcgctcggc ttcttcgtgt tcccgaggcc ggaaccgatg ccgcccttcg
      961 aggtcgcgcc cctggccggc ggcagggcgg gactgggcga cgcggccggc aaggtcgtcc
     1021 tgctcaactt ctgggccacc tggtgcccgc cctgccgtag cgagatgccg tccattcagc
     1081 gcctccacga cgccatggcc ggccggccct tcgcggtgat ggcggttagc gtagccgagt
     1141 cggccgcgac tgtgcgcgcc ttcctcaaga cggatcccta taccttcccg atctacctcg
     1201 acgagggagg ccgggcctcc ggccccttcg tgggccgcgg catacccacc accttcgtac
     1261 tcgacaagca gggccgggcg atagccggcg tcataggcgc ccggtcctac gacgaccccg
     1321 aggtgttggc gctgttcaag gcgctcgcgg agcgtctgtg atcgctgacg tatcgatact
     1381 cgtcgccctg ggcgcggggc tcctgtcgtt cctatcgcca tgcgtgctgc cgttgatccc
     1441 cggctatctg tcattcatca gcggtgccgg catcgagcgc atacgcgacg gttctgagcg
     1501 ctccggcgtc ttctggagga cgttgttttt cgtgaccggc ttctcggccg tgttcatagc
     1561 gctggggcta gcgttctcgg gcggcggcat gctgaccgcg gggcgcgcca accgcgtggt
     1621 gacggtcgcg gccggttcgc tcatcgcggc gctcgggctg aacatgatct tcggcttcct
     1681 acgcttcttg aacctcgaag ccagggtcaa ggccccggcc aagccgagga gcgccgccgg
     1741 tgccttcgtc gtcggcatgg ccttcggggc cggctggacg ccctgcgtgg ggcctatcct
     1801 ggcctcgata ctcgtaatgg ccgcacggtc cggtggggcc ggccgggcgg tcatgctcct
     1861 gacggcctat tcggccggct tggccctgcc gttcatcgcc gcgggcctgt tcttcgagcg
     1921 cttgtcaccg gtctgggcct ggttcaagcg ccatggacgc ggcgtccaga tcgtctcggg
     1981 cctcctactg ctcgctatcg gcgtatcgat ggccataggc cggctcggct cgataggcgc
     2041 gatcagcgcc agggccggca taggcttgaa ggccgccctg gccaccgatc cggcggccgt
     2101 caggctatgg agcgcggccg tcgtcgccgc gctcgcggct atcctggtcg tcgcgccgct
     2161 ggcgcggcgc cggcccttcg ccaagctatg gcgtatcgtc gcgctggccc tcctcgccgt
     2221 tctactgacg ctcgaggccg cgggcgccgt atcgatggcg agtctgatag ccgattggct
     2281 gttgttccag ggagcctagg ccgtaccggc cctgaacgag cgcctgaatc gcccgcgcct
     2341 gaggcgctcg agccagacga ccaggagggc ggcgacgatc ctgggcaggg taatccagcc
     2401 tagcggcgtg ccgatcgcca ggaacagcga cgtgtcctcg agcaggctgt ggcataggca
     2461 ggcgtggtgg ttgaacaggt cggcctcctg cggcttcatc gctccgccct ttacccgctc
     2521 catgatgatc gcggccccgt aggcgtagcc gacgacgtta atgacgatcc acaggaagct
     2581 ggagttatcc gagaggccga agacgcgcat gagcggggcg gccagcctcg aaaggaagtc
     2641 gagcacgcgg aacgcttcca tgagccgctg cgctatcatg atgccattga cgagcagcag
     2701 gatcttgacg accagcctga gcgtcgacag gccccacgcg ccgagagccg gaaggagcgc
     2761 ggcccgcgcc gtatcggccg tcgacgcggc gacggaggat gcgtctcccg gtagcagcag
     2821 attgagcgcg taggcgagcg cgacgccgcc gagtatcctg agtgacagca tcttgatcgc
     2881 cgaggagccc gacttccgca tcacggcggt ctcgatgatc aggttgtggg cggacaggca
     2941 cagcacggcc agaatcgtta tctcacgcat cgtcagcggt agcgtctcga tcaccgccac
     3001 ggcgctgtac acgttgagca gcatcgagct gacg
//
LOCUS       DAOOLN010000087         2986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_40254_length_2986_cov_2.976117, whole genome shotgun sequence.
ACCESSION   DAOOLN010000087 DAOOLN010000000
VERSION     DAOOLN010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40254_length_2986_cov_2.976117"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="PLW80_01760"
     CDS             complement(<1..1157)
                     /locus_tag="PLW80_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPB65253.1"
                     /translation="MPDYGTPAGLDEAELQKRVRHDYRLSEAEVEARLRAELPDLSAD
                     ELRRWSDSGALEWRMLDGEKRYFNRAVVNLWRLCPEARDRRAAHSGARPAGDTGVAGL
                     AFDLLDHMRRALDAAKATGPGYVMPIRIRLDYTLTVRPGAVPEGETLRCWLPLPRVGG
                     QHLDLAILATDPADATAAPEEAPQRTLYLERPASPAGSPTVFSASYEFTSRDYAPLVD
                     PERVRPTDASTELYREYTAERPPHLVLSPEIRGLASSIVGSEANPYRKARLIFAWFDR
                     NVTYTATPEYSTIPNLSEYCLRERRGDCGVQAMLFIALCRAVGVPARWQSGLTLLPGK
                     MNLHDWALFHVEPYGWLPADPSRGFRDTDDEELRWFYLGGIDAFRAVVNDD"
     gene            complement(1210..1503)
                     /locus_tag="PLW80_01765"
     CDS             complement(1210..1503)
                     /locus_tag="PLW80_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65254.1"
                     /translation="MSKLRTGIPVFSFNNALLNVLEDDYDDAYAVLAAYKEGPRDGGT
                     GDEGPGGEDARGVERIRNAAEIVLGGWKSPSPGERAIELYDRPKTPRSPRNQE"
     gene            complement(1460..2746)
                     /locus_tag="PLW80_01770"
     CDS             complement(1460..2746)
                     /locus_tag="PLW80_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65255.1"
                     /translation="MLKKTRSDATLIRDLPDFTRFMPFLMPNKGGSVINYEQDLDVTE
                     TLDLIKQVNRELIKEREILTLFGVVINAAVRAVAMRPKLNRFISGYRFWQRNQILVNF
                     VAKKEISDEGQEVNVKIDLSPYETLATTARKVRSAVKRAVSDDGAENEAVVSTIMRLP
                     DPIVKLLVRGMDWLDQRNLMPRSMTDSDPMWSSVFLTNVGSFGLDAPYHHLFERGNCP
                     VFLAMGKVREERLLDEEFRPVTRKMLRLRYTFDDRVADGVYMGRALDLVSVSWSTPTS
                     CWNRRYSAANCWPSCGSGITLPRPYRLGPPRFRPPRGFEHERQIRGGGSRAHLRGGAR
                     QVLQRASEEAPGRGGDAGGNARGWRRSPRGGRRDGARVPRRGGRRQARPQDSQRLQPV
                     RHHVRQVAVPVGADTVLLRVREHVQAAYRHTRVQFQ"
BASE COUNT          461 a          995 c          998 g          532 t
ORIGIN      
        1 tagtcgtcgt tgaccacggc cctgaacgcg tcgatgccgc cgagatagaa ccagcgtagc
       61 tcctcgtcgt ccgtgtccct gaagccgcgc gacgggtcgg ccggtaacca gccgtagggc
      121 tcgacgtgga acagcgccca gtcgtgcaag ttcatcttgc cgggtagcag ggtcaggccg
      181 gactgccagc gcgccggcac gccgaccgcg cggcacagcg cgatgaacag catggcctgg
      241 acgccgcagt cgccgcgccg ttcgcgtagg caatactcgg agaggttcgg gatagtcgag
      301 tactccggcg tggcggtata ggtcacgttg cggtcgaacc acgcgaagat cagcctggcc
      361 ttgcggtagg ggttcgcctc ggagccgacg atggacgagg cgaggccgcg gatctccggg
      421 ctcaggacca ggtggggcgg gcgctcggcc gtatactcgc gatacagctc cgtcgatgca
      481 tccgtgggcc gtacccgctc cggatcgacg agcggggcgt agtcgcggct cgtgaactcg
      541 tagctcgccg agaagaccgt cggcgagccg gccggtgacg cgggccgttc caggtacagc
      601 gtccgttgcg gcgcttcctc gggggccgcc gtcgcgtcgg cgggatcggt ggcgagtatg
      661 gccaggtcga ggtgctgccc tccgacgcgc ggcaacggta gccagcaacg tagcgtctcg
      721 ccctcgggta cggcgcccgg gcgcacggtc agcgtatagt cgagcctgat cctgatcggc
      781 atcacgtagc ccggacccgt ggccttggca gcgtcgagcg ctcgtctcat atgatcgagc
      841 aggtcgaacg cgaggccggc tacgcccgta tcccccgcag gcctggcgcc ggagtgggcc
      901 gcccggcggt cgcgcgcctc ggggcagagc cgccacaggt tgacgacggc ccggttgaag
      961 tagcgcttct cgccatccag catgcgccac tcgagggcgc cggagtcaga ccagcgccgc
     1021 agctcgtccg cgcttaggtc tggcagttcg gcgcgcagcc gcgcctccac ctcggcctcg
     1081 gacaggcggt aatcgtggcg cacgcgtttc tgtagctcgg cttcgtcgag gccagcgggc
     1141 gttccatagt cgggcatggc gtcctcccac gaccgttata ccacgcctgg gcgatccgcg
     1201 aggctaaaac tattcctggt tcctcggact tcgtggcgtc ttcggccggt cgtacagctc
     1261 gatggcgcgt tccccgggcg acgggctctt ccagcctccg aggacgatct cggccgcgtt
     1321 ccgtatccgc tcgacgccgc gcgcgtcctc gccaccgggg ccttcgtcgc ccgtcccgcc
     1381 gtcgcgcggc ccttccttgt aggccgcgag caccgcgtag gcgtcgtcgt agtcgtcctc
     1441 gagcacgttg aggagcgcat tattgaaact gaacacgggt atgccggtac gcagcttgga
     1501 catgttctcg aactcgaagt agtacggtat ctgctccgac tggaacagcg acttgacgaa
     1561 catgatgtcg tactggttgt agacgctggc tatcctgagg gcgcgcctgc cggcggccac
     1621 ctcgtcgagg aacacgcgct ccatctcgac gccctcctcg ggggctgcgg cgccaccctc
     1681 gagcgttccc gcccgcgtcg ccgcctcgtc ctggcgcttc ttccgacgct cgctgtagta
     1741 cctgacgagc gccgccccga agatgagcgc gagagccgcc gccacgaatt tgacgttcat
     1801 gctcgaaccc cctcggcggc cggaacctcg gcggcccgag cctgtacggc ctcggcaggg
     1861 taatccctga gccgcagctc ggccagcagt tcgcggctga gtatcggcgg ttccagcagc
     1921 tcgtcggcgt gctccacgaa acgctgacca ggtcgagcgc gcggcccatg tagacgccgt
     1981 cggcgacgcg gtcgtcgaag gtgtagcgca gcctgagcat cttgcgcgtc accgggcgga
     2041 actcctcgtc gaggaggcgc tcctctcgca ccttgcccat ggccaggaat accgggcagt
     2101 tgccgcgttc aaacaggtgg tggtagggcg cgtccagccc gaaggagccg acgttggtca
     2161 ggaagacgct cgaccacatc gggtccgagt cggtcatcga gcggggcatc aggttgcgct
     2221 ggtcgagcca gtccatgccg cgcacgagca gcttgacgat gggatcgggc agcctcatga
     2281 tggtcgagac gacggcctcg ttctcggcgc cgtcgtccga taccgctcgc ttgaccgccg
     2341 accgcacctt gcgcgcggtg gtcgcgaggg tctcgtaggg gctgaggtcg atcttgacgt
     2401 tcacctcctg cccctcgtcg ctgatctctt tcttggccac gaagttgacg aggatctggt
     2461 tccgctgcca gaaccggtag ccggagatga aacggttgag cttgggacgc atcgccacgg
     2521 cgcgcaccgc cgcgttgatc accacgccga acagcgtcag gatctcgcgc tccttgatca
     2581 gctcgcggtt gacctgcttg atgagatcca gcgtctcggt gacgtccagg tcctgctcgt
     2641 agttgatcac cgacccgccc ttgttcggca tcaggaaggg catgaagcgg gtaaagtcag
     2701 gcaggtctct aatcagcgta gcgtctgatc tcgtcttctt gagcatcgtt ccctctcggc
     2761 cgtcgtgtct cgcggttccg gaatcatagc acggccgggg gagggcgtca cgcgcccgga
     2821 accgacgcgt ataggcttag cgccctcccg agcgccgctt ccattcctcg agcaggatgc
     2881 gcagcttgaa cgcgttcatc acgccgtcgc ggctcgagcc gaagtagcgt ttatcgatgg
     2941 agaaatcgac gctcgagccg cgacggcgtg atgaacgcgt tcatca
//
LOCUS       DAOOLN010000088         2984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_40284_length_2984_cov_2.877433, whole genome shotgun sequence.
ACCESSION   DAOOLN010000088 DAOOLN010000000
VERSION     DAOOLN010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40284_length_2984_cov_2.877433"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="PLW80_01775"
     CDS             <1..973
                     /locus_tag="PLW80_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65256.1"
                     /translation="AGIRPLAVYGPVHYQELPELFMDFICSLTGKSPSTTGAGSEGAL
                     TKGPFNSLTPTSDLNNALLGFILGGYDGFSTAAGYIGHKYRVEHDISLLVPELWARLT
                     PEERDPAFLIENGYLERVEDFEHGGRTVPAGRLGWRVTPLFASHFLGRIFDAPSSVFP
                     EEVLRPELQSLEYFADGVLNIAEAQERVARDYLQDGSIEAAIPPLQAILRVMADGSWN
                     GKGLDDPELRRLFDRDYVLASDWYAARLEAYAEAERSRLREGVAYLEEFLGSPRGRDE
                     RDLAKARRELTTVQQSLERASRPEYAASLVGTIGVDPLFRSGLGAGR"
     gene            970..2220
                     /gene="hutI"
                     /locus_tag="PLW80_01780"
     CDS             970..2220
                     /gene="hutI"
                     /locus_tag="PLW80_01780"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HPB65257.1"
                     /translation="MRATLLIENIGELATPLGSTARSGAAMRQLRIVRDAVVAVDVET
                     IAWAGPASELPRLEGPPPERLDARGKAVVPGFVDSHTHFVFGGYRDEEFFWRAEGLPY
                     MEIHARGGGIANSVRDTRAASAEELTAAAVARLERMLELGVTTVEGKSGYGLDLETEL
                     RQLEVMRLLDSRQPVDIVSTYMGPHSVPAEFKGNPSGYIDFVLREVMPRVKEGGLARF
                     CDVFCERGVFELDDSRRFLEAAKSMGFGVKLHADEIVRLGGAGLAAEIGAVSADHLLK
                     ASEDDLLAMAEAGVVATCLPLTAFSLREPYAPARAMIDRGLAVAVASDLNPGSCYSQA
                     IPLAAALSVLYMGMTIEETLTALTLNAAAALSLAGDRGSIEAGKLADLLVLDAPSYRH
                     LAYHVGMNVVGTVVKRGKVVSVRT"
     gene            2249..>2984
                     /locus_tag="PLW80_01785"
     CDS             2249..>2984
                     /locus_tag="PLW80_01785"
                     /inference="COORDINATES: protein motif:HMM:NF025694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4392 domain-containing protein"
                     /protein_id="HPB65258.1"
                     /translation="MSVSTYEAAGPASIEAAMAFDPGGRGLCGDPRLRVALRGSIRRA
                     AARLAGADRVLIVTGFLIERAGVGESDGPPGSAVLARALVRLGKDVHIVTDQYSAALV
                     EASAAALGSRPRVVTLSPGDDEPACIDILSGFRPDVVVAIERPGKAADGGFYSMRGSD
                     MRALVPDTDALVARASDYGGVSVAIGDGGNELGMGSIGAIIDERVPLGSTIRAVTPAD
                     EPIVAGVSNWGAYGLVRAFEKHENVPA"
BASE COUNT          456 a         1036 c          998 g          494 t
ORIGIN      
        1 ggcgggcatc aggccgctgg ccgtctacgg cccggttcat tatcaggaac tgcccgagct
       61 gttcatggac ttcatctgct ccctgaccgg caagagcccg tccacgacgg gggccggatc
      121 cgagggagcg ctgaccaagg gcccgttcaa ctcgctgacg ccgacctccg acctgaacaa
      181 cgcactgctc ggcttcatac tcgggggcta cgatggtttc tcgacggccg ccggatacat
      241 aggccacaag taccgcgtcg aacacgacat ctcgctgctc gtgcccgagc tgtgggccag
      301 attgactccc gaggagcgcg acccggcctt cctgatcgag aacggctacc tcgagcgcgt
      361 cgaggacttc gagcatggcg gccgaaccgt acccgcgggc cgcctcggct ggcgcgtcac
      421 accgctgttc gcgtcccact tcctcggccg catcttcgac gccccatcgt cggtgttccc
      481 cgaggaggtg ctccggcccg agctgcagag cctcgagtac ttcgccgacg gcgtgctcaa
      541 catagccgag gcccaggaac gcgtcgctcg cgactacctc caggatggtt cgatcgaggc
      601 ggccatcccg ccgctccagg ccatcctacg cgtgatggcg gacggttcct ggaacggcaa
      661 gggtcttgac gaccccgagc tacggcgctt gttcgatcgc gactacgtac ttgcgtccga
      721 ctggtacgcc gctcgcctcg aggcctacgc cgaggccgag cgatcgaggc tacgcgaggg
      781 tgtcgcctac cttgaggaat tcctaggctc gcctcgcggc cgtgacgagc gcgacctcgc
      841 gaaggcccgt cgcgagctga cgaccgtcca gcagagcctc gagcgcgcgt cgcggcccga
      901 gtatgccgcc tctctcgtag gcacgatcgg cgtcgacccg ctgttccgct ccggactggg
      961 agctggacga tgagggctac gctactgatc gagaacatcg gcgagctcgc cacacctctc
     1021 ggctcgacgg cgcgctcggg cgccgccatg cgccaactac ggatagtccg cgacgcggtc
     1081 gtcgccgtgg acgtggagac gatagcctgg gccggtccgg cctccgagct accgcggctc
     1141 gagggtccgc cgccggagcg cctcgacgcg cgcggcaagg ccgtcgtacc gggtttcgtc
     1201 gactcgcaca cccatttcgt gttcggcggc taccgcgacg aagaattctt ctggcgggcc
     1261 gagggcctgc cgtacatgga gatccacgcg cgcggaggcg gcatcgccaa ctcggtacgc
     1321 gataccaggg cggcctcggc cgaagagctg acggccgccg ccgtcgcccg cctcgagagg
     1381 atgctcgagc tcggcgtcac caccgtcgag ggcaagtctg gctacggcct cgacctggag
     1441 accgagcttc gtcagctcga ggtgatgcgg ctactcgact ccaggcagcc cgtcgatatc
     1501 gtatcgactt acatgggtcc gcattccgtc ccggccgaat tcaagggaaa cccgtccggc
     1561 tacatcgact tcgtcctacg cgaggtcatg ccccgggtca aggaaggcgg cctggcgcgt
     1621 ttctgcgacg tcttctgcga acgcggggtc ttcgaactcg acgactcgag gcgcttcctc
     1681 gaggcggcca agtccatggg cttcggcgtc aagctgcacg ccgacgagat cgtcaggctc
     1741 gggggagccg ggctagccgc cgagatcggc gcggtcagcg ccgatcacct tctcaaggct
     1801 tccgaagacg atctcctggc catggcggag gccggcgtcg tggcgacctg cctgccactt
     1861 acggcgttca gcctacggga gccgtacgcc cccgccaggg cgatgataga ccgagggctg
     1921 gcggtcgcgg tcgcgtcgga cctcaatccc ggctcgtgct acagccaggc cattcccctc
     1981 gccgcggcct tgtcggtgct gtacatgggc atgactatcg aggagacgtt gaccgcgctg
     2041 accctgaacg ccgccgccgc gctctccctg gcaggcgacc ggggctcgat agaggccggc
     2101 aagctggccg acctgctcgt gctggacgcc ccgtcatacc gccacctggc gtaccacgtg
     2161 ggcatgaacg tcgtcggtac cgtcgtcaag cgcggcaagg tcgtcagcgt ccggacctag
     2221 tcgccccaaa cgggctacga ggagtgtcat gagcgtttca acttatgagg ccgccggccc
     2281 ggcgtcgatc gaggccgcga tggctttcga cccgggcggc cgaggcctat gcggcgaccc
     2341 gcggttacgc gtcgcgctac ggggttctat ccggcgggcc gcggccaggc tggccggggc
     2401 cgacagggtc ctgatcgtca ccggcttcct tatcgagcgg gccggtgtcg gggagagcga
     2461 cggcccgccc ggctccgccg ttttggcgag ggccttggtc cgcctcggca aggacgtcca
     2521 tatagtcacc gaccagtact cggccgcctt ggtcgaggcg tcggccgccg cgctcggctc
     2581 gaggcctcgc gtcgtaacgc tgtctccggg cgacgacgag cccgcctgta tcgacatcct
     2641 gtcgggcttc cggccggacg tcgtcgtggc catcgagcgg ccaggcaagg cggccgacgg
     2701 cggcttctac tcgatgcgcg gctccgacat gcgagcgctc gtacccgaca ccgatgcgct
     2761 cgtggccagg gcgtccgact atggcggcgt ctccgtcgcc atcggcgatg gaggcaacga
     2821 gctggggatg ggctccatcg gggccatcat agatgagcgc gtcccgctcg gttcgacgat
     2881 tcgggcagta acgcccgccg acgagcctat cgtagccggc gtgtccaact ggggcgctta
     2941 cgggctcgtc agggccttcg agaagcatga gaatgttccc gccg
//
LOCUS       DAOOLN010000089         2980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_40348_length_2980_cov_3.292650, whole genome shotgun sequence.
ACCESSION   DAOOLN010000089 DAOOLN010000000
VERSION     DAOOLN010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40348_length_2980_cov_3.292650"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PLW80_01790"
     CDS             <1..395
                     /locus_tag="PLW80_01790"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HPB65259.1"
                     /translation="MPPAMSISSIRILKDELINTKLGVELVVSNPNAFALTFAELDYR
                     LYGEGRYWASGCLAKPFVVPAGEATKASLYLTMNFTDMSRSLLDQVIKLATVSYRLVG
                     SGRVDTGLEFLPRFELPFDMSGRAEVGR"
     gene            complement(392..1990)
                     /gene="purH"
                     /locus_tag="PLW80_01795"
     CDS             complement(392..1990)
                     /gene="purH"
                     /locus_tag="PLW80_01795"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010699651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPB65260.1"
                     /translation="MPIALISVSDKTGLDAFAGRLEKAGWRLLASGGTAKAIAAAGVA
                     VDEVADYTGSPEILGGRVKTLHPAIHGGILSRGTDADRSELAGLGWSELDLVVCNLYP
                     FEATMARPGAAEAACVEEIDIGGVALLRAAAKNYARVAVVCDPADYERVAASLETSVG
                     ESGGLGPELRRELAVKAFALCARYDAAIASWLEPESSVGPFGYAGQKLRYGENPHQKA
                     WLYTDEPGAGPLGGSVLQGKELSYNNLLDLDAAWRTAAGFDGAAAVVVKHLSPCGVAE
                     SGVAESGVAESGVATTDGESPARALEAAVACDPLSAFGGVVAVNRSFDADSVRALGET
                     FVECVAAPSFTPEARGLLAKRKNARLLEMGDAPSRTAVGYETRSIRGGFLRQEIDDGD
                     PDGTEWKVVSKRAPTEAELAALRFAWKACVSAKSNAILLAAPIDAADPSAGYATVGIG
                     SGQPNRVDSTRIAVGRAGDKARGSVLASDAFFPFPDSIEVAAAAGVSAIAHPGGSIRD
                     GLSVEAADAAGIAMIVTGVRHFRH"
     gene            complement(2066..2734)
                     /gene="purN"
                     /locus_tag="PLW80_01800"
     CDS             complement(2066..2734)
                     /gene="purN"
                     /locus_tag="PLW80_01800"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPB65261.1"
                     /translation="MSGPGRRLRVVVLASGNGTNLQALLDASAAGALRAEVAAVVSDR
                     TGAYALERARLAGLTAVEAPFERGGDRRAYDAALAKAVATHDPDYVLLLGWMRILSSA
                     FLTRFPGRVVNLHPALPGTFPGVRAIERAFEAYRTGAIDRTGVMTHLVPDEGVDSGPV
                     LAVEEVPILDGDDLGSLEARVHAVERRLLVKTVKGLVRKNNHGGTETRRKTEESGGEG
                     YGIP"
     gene            complement(2731..>2980)
                     /locus_tag="PLW80_01805"
     CDS             complement(2731..>2980)
                     /locus_tag="PLW80_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPB65262.1"
                     /translation="AVVRRGSWPVPPLYGRIERLGRVEPDEMYRVFNMGVGMVAVVDS
                     GRVDELRALVGEECWVIGELVGSAQGGKARARLDGERA"
BASE COUNT          460 a         1056 c         1008 g          456 t
ORIGIN      
        1 tcatgccgcc cgcgatgagc atctcgtcga tacgcatcct caaggacgag ctgatcaaca
       61 cgaagctcgg cgtcgagctg gtggtgagca acccgaacgc gttcgcgctg accttcgcgg
      121 agctggacta ccggctgtac ggcgaggggc gctactgggc gtccggctgc ctggccaagc
      181 ccttcgtcgt cccggccggc gaggcgacga aggccagcct ctacctgacg atgaacttca
      241 ccgacatgag ccgatccctg ctcgaccagg tgatcaagct ggcgaccgtc agctaccgcc
      301 tggtcggttc cggccgcgtc gacaccgggc tcgagttcct gccgcgcttc gagctgccct
      361 tcgacatgtc cggccgggcc gaggtcggcc gctagtgcct gaagtgcctg accccggtga
      421 cgatcatcgc gatgcccgcg gcgtcggccg cctcgacgct taacccatcg cggatggagc
      481 cgcccgggtg cgcgatcgcg cttacgccgg ccgccgccgc cacctctatc gagtcgggga
      541 acgggaagaa cgcgtccgag gccagcaccg agccgcgcgc cttgtccccg gcccgcccta
      601 cggcgatgcg ggtcgagtcg acgcggttcg gctggccgga gcctatgccg actgtcgcgt
      661 agccggcgct cgggtcggcc gcgtcgatag gcgcggccag caggatggcg ttggacttgg
      721 ccgacacgca ggccttccat gcgaagcgta gcgccgcgag ctcggcctcg gtcggagctc
      781 gcttggagac gaccttccac tcggttccgt cgggatcccc gtcgtcgatc tcctgcctga
      841 ggaaaccgcc gcgtatagag cgcgtctcgt atccgaccgc ggttcgcgag ggggcgtctc
      901 ccatttcgag caggcgggcg ttcttgcgct tggcgagtag cccgcgagcc tcgggcgtga
      961 acgacggggc ggcgacgcac tcgacgaagg tctccccgag cgcgcggact gagtcggcgt
     1021 cgaaggaccg gttgaccgcc acgacgccgc cgaaagcgga cagcgggtcg caggcgacgg
     1081 ccgcttcgag ggcgcgagcc ggcgactcgc cgtccgtcgt ggcgacgccg ctttcggcga
     1141 cgccgctttc ggcgacgccg ctttcggcga cgccgcaggg cgataggtgc ttgacgacga
     1201 ccgcggccgc cccgtcgaaa ccggcagccg tccgccaggc cgcgtccagg tcgagcaggt
     1261 tgttgtacga cagctccttg ccctggagca cgctgccgcc caggggcccg gcgcccggct
     1321 cgtcagtgta gagccaggcc ttttggtgcg ggttctcgcc gtagcgcagc ttctggccag
     1381 cgtaaccgaa cggccccacg ctggattccg gctcgagcca cgaggcaatg gccgcgtcgt
     1441 agcgggcgca tagcgcgaag gccttgaccg ccagctcgcg cctgagctcc ggcccgaggc
     1501 ctccagactc gccgacgctc gtttcaagcg aggccgcgac gcgctcgtag tccgcgggat
     1561 cgcagacgac ggcgacgcgc gcgtagttct tggcggcggc gcgcaggagg gcgacgccgc
     1621 ctatgtcgat ctcctcgacg caggccgcct cggctgcgcc tggccgcgcc atcgtcgcct
     1681 cgaagggata gaggttgcat acgaccaggt cgagctccga ccagccgagg ccggccagct
     1741 ccgagcggtc ggcgtccgtg ccgcgtgaca ggatgccgcc gtggatcgcg gggtgcagcg
     1801 tcttgacccg gccgcccagg atctccggcg agccggtgta gtccgcgacc tcgtcgacgg
     1861 ccacgcccgc cgcggcgatg gccttggccg taccgccgga ggcgaggagg cgccagccag
     1921 ccttttccag ccgccccgcg aacgcatcga ggcccgtctt gtcggatacc gatatcaacg
     1981 ctataggcat acacgctcct cgaaacaaaa attaggaatt tgaaccaaag aaacacggag
     2041 gcacagagaa aaaagaatca ataagtcatg gtattccata tccttctcct ccgctttcct
     2101 ccgtcttcct ccgcgtctcc gtgcctccgt ggttgttttt gcgtaccaag ccttttacgg
     2161 tttttactaa aaggcggcgc tcgacggcgt gtacccgcgc ctcgagcgat ccgaggtcgt
     2221 caccgtccag gatgggtacc tcctcgacgg ccaggaccgg cccggagtcg acgccctcgt
     2281 cgggcacgag gtgggtcatg acgccggtgc ggtcgatggc gccggtccgg taggcctcga
     2341 aggcccgctc gatcgcgcgc acaccgggga aggtgccggg cagggcagga tgcaggttga
     2401 cgacgcggcc cgggaagcgc gtcaggaagg ccgacgatag gatgcgcatc cagccgagta
     2461 gcagcacgta atcagggtca tgcgtcgcta cggctttcgc gagcgcggcg tcgtaggccc
     2521 gccggtcgcc gccgcgctcg aacggcgcct cgacggcggt aagtcccgcc agtctcgcgc
     2581 gctcgagcgc gtaggcgcca gtcctgtcgg aaacgacggc agctacctcg gcccgcaagg
     2641 ctccggcagc cgaggcgtcg agcagggctt gcagattcgt gccgttcccg gacgcgagga
     2701 ccacgaccct caggcgacgt ccggggccgc tcacgctcgc tccccatcga gtcgcgcgcg
     2761 agccttcccg ccctgcgccg agccgaccag ctctccgatc acccagcact cctccccgac
     2821 gagcgcgcgc agttcgtcga cccggccgct gtcgacgacg gccaccatgc cgacgcccat
     2881 gttgaacacg cggtacatct cgtcgggctc gacgcggcca aggcgctcga tacggccgta
     2941 cagcggcggt accggccagg agccgcgcct gaccacagcg
//
LOCUS       DAOOLN010000090         2974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_40475_length_2974_cov_2.864680, whole genome shotgun sequence.
ACCESSION   DAOOLN010000090 DAOOLN010000000
VERSION     DAOOLN010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40475_length_2974_cov_2.864680"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(391..2829)
                     /gene="pbpC"
                     /locus_tag="PLW80_01810"
     CDS             complement(391..2829)
                     /gene="pbpC"
                     /locus_tag="PLW80_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HPB65263.1"
                     /translation="MALAWATTAAKARRGATIVLAWAARPDTAPALAGAALFVLLFMA
                     FEPPTLAPAAYSSALYAASGELLGAKVAPDGQWRFEPSGEVPERYAVALVEYEDGRFY
                     GHAGFDPLAIARAARLNARSGRVVSGGSTVTMQAARLSKAPGPRDLGAKIVELWTAVR
                     LEFGQTKAEILAGYAGMAPYGGNVVGLEAAGFRWFGRPSEALTWAEAATLAVLPNSPG
                     LVHPGRSRDELRRKRDRLLSSLADAGRIDAEALAVALAERLPEEPLPMPDLAPHLLAK
                     TGPGRTVSTIDGRIQTVAVEIASRHSRRLGRNGVANLAIVVLRVDDGSVAAYVGNSPG
                     AGTDEARWVDCADSPRSSGSILKPFLYAAMIDSGELAPGRLVLDIPTRVGSYSPENNL
                     KTYSGAVRADEALARSLNVPFVRLLRSYGVERFASLLKRLGFSTLSRAPADYGLPLIL
                     GGAEATLIDAASAYASLARMAKADDPAPPVQSAGATWRSGDDAGDDAGDDAGDDAGDD
                     AGDGAGRSMFEPQFSAGAAWLTLEALVSVARPGEEASWQEFASSRRVAWKTGTSFGSR
                     DAWAIGATPEYVVAVWAGNADGSGRPELKGTDAAAPILFDTFQALPRSGWFAEPVRSL
                     RWETVCAESGYPAGPDCATTDRVLVPAASKADQPCPYCRTVSLSADGRYRVRAEEVGG
                     AELLVERRFALPPAVEWYYARSSPGYRPLPPWLPGSAGAEADEIEFLAPELGASLYVP
                     IELDGTPGKAVFRAAHRDRAARLFWHLDGVYLGETVVDHRVEARPAPGFHELVVVDGH
                     GSTASRRFEVYDRE"
BASE COUNT          445 a         1042 c         1078 g          409 t
ORIGIN      
        1 agagcgagac cgggcgcgat cggacgctcg cgtccttggg caggatcgcc gagtatccgg
       61 gctcgatcag cacgtcgtcg tacgaaaggg tctcgcgaat ctccatgggg cctcctcggt
      121 caatcgtatc ggcgtccgcc gacgatcgcc cgcggaccct cccgccgcgc tcggcgcggc
      181 gtgtccgcct aggtgcgtcc ggctcccgag agccggcgcc caagtcccgt agccccgggc
      241 gcgcccaaac cgacgctctg tcggcgtcga cggacggggc ctctgaaatg ttcgatgggg
      301 tgcatagcgg gttatacatc ggaatagtcg acgggcgcaa gcccggcagg cacgaaccgc
      361 tcggggatgc gaaccggcgc gctagaccgg ttactcccgg tcgtagacct cgaagcgccg
      421 cgaggcggtc gagccatgac cgtcgacgac gaccagctca tggaagcccg gagccggcct
      481 cgcctcgacg cggtggtcga cgaccgtctc gccaaggtag acgccgtcga ggtgccagaa
      541 cagccttgcc gcccgatcgc gatgggccgc gcggaacacg gccttgcccg gcgtcccgtc
      601 gagctcgatc ggcacgtaca gcgaggcccc gagctccggc gctaggaact cgatctcgtc
      661 agcttcggct ccggcagatc cgggcagcca gggcggtagc ggccgatagc ccgggctcga
      721 ccgagcatag taccactcga ccgcgggagg caacgcgaaa cgcctctcga cgagcagttc
      781 cgcgccgccg acttcctccg ccctgacgcg gtagcggccg tcggcgctca gcgataccgt
      841 ccggcagtac ggacagggtt ggtctgcctt cgaggccgcc ggtacgagta cgcgatcggt
      901 cgtcgcgcag tcggggccag cgggatagcc ggactcggcg cagaccgtct cccatctcag
      961 ggatcgcacc ggctcggcga accagcccga gcggggtagc gcctggaacg tatcgaacag
     1021 gatgggcgcg gcggcgtcgg tgcccttgag ctccggtcgg cccgagccgt cggcgttgcc
     1081 cgcccagacg gcgacgacgt actccggcgt cgcgcctatg gcccaggcgt cgcgcgagcc
     1141 gaagctggtg ccggtcttcc aggcgacgcg ccgtgagctg gcgaattcct gccaggaggc
     1201 ctcctcgcct ggcctggcca cactcacgag ggcctcgagc gttagccagg ccgcgccggc
     1261 cgaaaactgc ggctcgaaca tggagcggcc cgcgccgtcg cccgcgtcgt cgcctgcgtc
     1321 gtcgcccgcg tcgtcgcctg cgtcgtcgcc cgcgtcgtcg cccgaacgcc aggtagcgcc
     1381 ggcagactgc acgggcggcg caggatcgtc ggccttggcc atccgcgcga ggctggcgta
     1441 ggccgaggcc gcgtcgatga gggtcgcctc tgcgccgccg agtatcaggg gcagaccgta
     1501 gtcggctggc gctctcgaca gcgtcgagaa gccgagccgc ttgaggagcg aggcgaaacg
     1561 ctcgactccg tacgagcgga gcagcctgac gaagggcacg ttgagcgacc tggctagcgc
     1621 ctcgtcggcc cgcaccgcgc ccgaataggt cttgagattg ttctccgggc tgtacgagcc
     1681 gacccgcgtc ggtatatcga ggacaaggcg gccgggcgct agctcccccg agtcgatcat
     1741 ggcagcgtac aggaaaggct tgagaatgct ccccgagcta cggggagagt cggcgcagtc
     1801 cacccagcgg gcctcgtcag tcccggcccc tggcgagttg ccgacgtagg ccgcgacgct
     1861 accgtcgtcg acgcgtagga cgacgatggc gagattggcg acgccgttcc gcccaaggcg
     1921 cctcgagtgc ctcgaggcga tctccaccgc cacggtctgg atcctgccgt cgatggtcga
     1981 gacggtgcgc cccgggccgg ttttggcaag caggtggggc gcgaggtcgg gcatggggag
     2041 gggctcctcg ggcaggcgct cggccagggc gacggcgagc gcctcggcgt cgatacggcc
     2101 tgcgtcggcc agggaggaca ggagccggtc ccgcttgcgg cgcagctcgt cccgcgagcg
     2161 gccggggtgc acgaggccgg gactgttcgg caacacggcg agcgtggcgg cctcggccca
     2221 ggtgagcgcc tcggaaggcc ggccgaacca gcggaaaccg gcggcctcga gcccgacgac
     2281 gttaccgccg tagggcgcca tgccggcata cccggccagg atctccgcct tggtttggcc
     2341 gaattccagc cttacggcgg tccataattc gacgatcttg gccccgagat cgcgcggccc
     2401 gggagccttg gacagcctgg cggcctgcat ggtgacggtc gatcctccgg agaccacgcg
     2461 gccgctcctg gcgttgagcc tggcggctcg ggcgatggcc agcgggtcga agccggcgtg
     2521 gccgtagaag cgcccgtcct cgtactcgac gagggcgacc gcgtagcgtt cgggcacctc
     2581 gcccgacggc tcgaaccgcc actgcccgtc tggagccacc ttggccccga gtagctcgcc
     2641 cgacgcggcg tacagggcgc tcgaatacgc cgccggcgcc agggtcggcg gctcgaacgc
     2701 catgaacagg aggacgaaca gggcggcccc ggccaaggcc ggggccgtgt cgggccgggc
     2761 cgcccaggcc agaactatcg tcgcaccgcg ccgggccttg gcggccgtcg tcgcccaggc
     2821 cagcgccatc ggcgcaagcc tacggttcaa ggcctttggc cgcggctatt cgggttggcc
     2881 cgctgctcgg cgacgggctt gggcaagggc cggcccggca gcaccgcgaa caccgtcggg
     2941 tcgtacatcg cctcggcggt gaaggccggt agga
//
LOCUS       DAOOLN010000091         2953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_40864_length_2953_cov_2.921325, whole genome shotgun sequence.
ACCESSION   DAOOLN010000091 DAOOLN010000000
VERSION     DAOOLN010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40864_length_2953_cov_2.921325"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="PLW80_01815"
     CDS             <1..720
                     /locus_tag="PLW80_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPB65264.1"
                     /translation="VAATNEQASREQIEADIAALSGFDGTVLFYYSGHGIAEMRGESA
                     ICPYGSIDSSGYLVASEMITASELRGMFESAGLGRVVVVLDSCNSGGFVAAGATTDAI
                     PGSYGIYDDELAYTWYLGALGDAAAAFSYAAEPGYVVLAAAGADEYSWESGDHGIFTR
                     FLLEAAGDGSEAADSDGDGYLTTTELYRYALAGIEAEWNHIYDDTPSLRIQLYAGDYW
                     DDYLPHLSGTAIEYALMPAGD"
     gene            complement(791..994)
                     /locus_tag="PLW80_01820"
     CDS             complement(791..994)
                     /locus_tag="PLW80_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PC4/YdbC family ssDNA-binding protein"
                     /protein_id="HPB65265.1"
                     /translation="MDRRLAVLGPSGKDWTVELNLVSWNGREPKLDLRSWDPSHERMG
                     KGIALTRDEAEKLRDALADYLAT"
     gene            complement(1046..1558)
                     /locus_tag="PLW80_01825"
     CDS             complement(1046..1558)
                     /locus_tag="PLW80_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HPB65266.1"
                     /translation="MYYNTAIAYLLWALGGFGALGFHRFYLRKIPTGILWLCTGGLGM
                     VGSIYDFFTLSGQVREANIRAGYREALDMPRERVVIRERYVEPSQDRAHAKESIEKVI
                     LRTAKKNGGLVTPGEIAIESDYDSEAARSALEKLAAKGFCEMRVRPSGVIVYRFPEFA
                     AGDEGFEPGM"
     gene            1668..>2953
                     /gene="asnS"
                     /locus_tag="PLW80_01830"
     CDS             1668..>2953
                     /gene="asnS"
                     /locus_tag="PLW80_01830"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPB65267.1"
                     /translation="MKDIKAILAERSVGSAITAKGWARTKRSTKGATFVELYDGSCFS
                     GLQCVFDSGAAAPGLEATLARVTTGAALAVSGVLVDSPAAGQPVELKASAVEVIGECP
                     ADSYPLQKKAHSLEFLREIAHLRPRTNTIGAAARMRDEMAYAVHTFFHDRGFRYVHTP
                     LITASDCEGAGEMFQVTTLDLDAIAKRGEAVDYAKDFFGKKAFLSVSGQLNVETYCMA
                     LGKVYTFGPTFRAENSNTTRHLAEFWMIEPEFAFAELADDMELAEDFVKHLLGWALDR
                     CGDDMAFFDKRVQPGLIEMLDGVRSKRFAHMSYTDAVAELERRKEQFEFKPYWGCDLQ
                     TEHERYLSETVVGGPVIVTDYPKELKAFYMRLNDDGKTVRAMDVLVPRIGEIIGGSER
                     EYRYDVLVENMRAKDLAPEDYWWYLDLRRFGSAPHAG"
BASE COUNT          521 a          966 c          963 g          503 t
ORIGIN      
        1 gtagcggcca cgaacgagca ggcgagccgc gagcagatcg aggcggacat agcggcccta
       61 tcgggcttcg acgggacggt gctgttctac tattcgggtc acgggatagc ggaaatgcgc
      121 ggcgagtcgg ctatctgccc atacggatcg atcgactcga gcggttacct cgtggcgtcg
      181 gagatgatca ccgcttccga gctgcgcggt atgttcgagt cggccgggct cggccgcgtc
      241 gtcgtcgtgc tcgacagctg caattccggc ggcttcgtcg ccgccggagc cacgaccgac
      301 gcgatacccg gctcgtacgg tatctacgac gacgagctgg cctatacctg gtacctgggc
      361 gccctaggcg acgccgcggc ggccttctcc tacgcggccg agcctggcta cgtcgtgctg
      421 gcggccgccg gagcggacga gtactcctgg gaatcgggcg accacggcat cttcacgcgc
      481 ttcctgctcg aggccgccgg cgacggctca gaggcggccg acagcgacgg cgacggctat
      541 ctgacgacga ccgagctata ccgttacgcg ctcgcgggca tagaagccga gtggaaccac
      601 atctatgacg atacgccgag cttgcggata caactatacg ccggcgatta ctgggacgac
      661 tacctgcccc atctttcggg caccgcgata gagtacgcgc tcatgcccgc cggggattga
      721 gccggccgat agacgaacgg aggggccggc cgcgcggagc gcggccgggc gcgggcccga
      781 cccgcgcttc ctaggtggcc aagtagtccg cgagagcgtc ccggagcttc tcggcctcgt
      841 cgcgcgtcag ggctatgccc ttgcccatgc gctcgtggga agggtcccag ctacgcaggt
      901 ccagcttagg ctcgcgcccg ttccaggaga ccaggttgag ctcgaccgtc cagtccttgc
      961 ccgacggccc gagcacagcc aggcggcgat cgatccggaa ttgaatcaag ttgcggttcc
     1021 tatccactcc gtacctccac gctgtttaca tacccggctc gaagccctcg tctccggcgg
     1081 cgaactcggg gaatcggtag acaatgacgc ccgacgggcg cacgcgcatc tcgcagaagc
     1141 ccttggcggc tagtttctcg agagcactcc tcgcggcctc cgagtcgtag tcgctctcga
     1201 tggcaatctc gcccggggtg acgagtccgc cgttcttttt ggcggtgcgc aggatgacct
     1261 tctcgatcga ttccttggcg tgcgcgcgat cctggcttgg ctcgacatag cgctcgcgga
     1321 tgacgacgcg ctcgcgcggc atgtcgaggg cctcgcggta gccggcgcgg atgttggcct
     1381 cgcgcacctg ccccgacagc gtgaagaagt cgtagatgga gcctaccatg ccgaggccac
     1441 cggtgcagag ccagagtatg ccggtaggta tcttgcgtag gtagaaccta tggaaaccca
     1501 gggcgccgaa gcctccgagc gcccagagca ggtaggcaat cgcggtgtta tagtacattg
     1561 atcactccgt acgatggatg cgtatatagt accacagaat ctcccgatgg aaaaataccg
     1621 cgcgcggggc tttgaccaag gacggcgcct agtggtatta ctatgctatg aaggatatta
     1681 aagcgattct ggcggagcga tccgtaggct ccgcgatcac ggccaagggt tgggcccgca
     1741 ccaagcgctc gaccaagggc gccacattcg tcgagctgta cgacggctcg tgcttctccg
     1801 ggctccaatg cgtcttcgac tccggagccg cggcgcccgg gctcgaggcg actcttgcca
     1861 gggtgacgac cggcgcggcc ctcgccgtca gcggcgtgct cgtcgactcg ccggccgctg
     1921 gccagcccgt ggagctcaag gcctcggccg tcgaggtgat cggcgaatgc ccggccgact
     1981 cgtacccgct ccagaagaag gcccactcgc tcgagttcct gcgcgagata gcccacctgc
     2041 gcccgcgcac caatactata ggcgcggcgg cgcgcatgcg cgacgagatg gcctacgccg
     2101 tccatacctt cttccacgac cgcggcttcc gttacgtgca tacgcccttg atcaccgcca
     2161 gcgactgcga gggcgccggc gaaatgttcc aggtgaccac cctcgacctg gacgcgatag
     2221 ccaagcgcgg cgaggccgtc gactacgcca aggacttctt cggcaagaag gcctttctgt
     2281 ccgtctccgg gcagctgaac gtcgagacct actgcatggc cctcggcaag gtgtatacct
     2341 tcggtccgac cttccgagcc gagaattcga acacgacgcg tcacctggcg gagttctgga
     2401 tgatagagcc ggaattcgca ttcgccgagc tggccgacga catggagctg gccgaggatt
     2461 tcgtcaagca cctcctcggc tgggccctgg accgctgcgg cgacgacatg gcgttcttcg
     2521 acaagcgggt gcagccgggc ctcatcgaga tgctcgacgg cgtgcgttcc aagcgcttcg
     2581 cccacatgag ctacaccgac gccgtagccg agctggagcg acgcaaggag cagttcgagt
     2641 tcaagccgta ctggggctgc gacctccaga ccgagcacga gcgctacctg tccgagacag
     2701 tcgtgggcgg cccggtgatc gtgaccgact acccgaagga gctcaaagcc ttctacatgc
     2761 ggctgaacga cgacggcaag accgtgcgcg cgatggacgt gctggtgccg cgcatcggcg
     2821 agattatcgg cggctcggag cgcgagtacc gctacgacgt gctcgtcgag aacatgcgcg
     2881 ccaaggacct ggcgcctgag gactactggt ggtacctgga cctcaggcgc ttcggctcgg
     2941 cgccccacgc ggg
//
LOCUS       DAOOLN010000092         2936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_41207_length_2936_cov_2.668865, whole genome shotgun sequence.
ACCESSION   DAOOLN010000092 DAOOLN010000000
VERSION     DAOOLN010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41207_length_2936_cov_2.668865"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(471..1226)
                     /locus_tag="PLW80_01835"
     CDS             complement(471..1226)
                     /locus_tag="PLW80_01835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPB65268.1"
                     /translation="MNMLFIEAGEAGLALGRGDPRRKHIRSVLKKGPGDELAAGRADG
                     RLGVARIEADDEDGMTLSFAPGPEAPPLRPVTLLLGFPRPIQANRILKDLASLGVARI
                     MLSGTELGEKSYFQSDFFKNREFRSALIEGAEQAGNPRLPVVETAWTLARALDAIVPE
                     ADDAARDGASRFWAFDPYRAERPFGSVALSDSGPERPLVLAIGSERGWTDAELDLLSA
                     SGFPFASLGDRILKTETACVVAVAMALGKLGYI"
     gene            complement(1595..2788)
                     /locus_tag="PLW80_01840"
     CDS             complement(1595..2788)
                     /locus_tag="PLW80_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-2-enoyl-CoA reductase family protein"
                     /protein_id="HPB65269.1"
                     /translation="MKPMIRGNICLNAHPEGCAAMVENWIARAKADAPAMTAAALAAG
                     RPLPRATLVLGCSTGYGLASRIAAAFGCGAATVGVSLEREPSDKKAGTPGWYDNRAFD
                     AACTRDGLFSATIDADAFADATRAAVVALARECGLVFDQVVYSLASPVRVDPATGVMH
                     RSVLKPLGEPFTGRTFDVVDGRMSEVTIQPATDEEVEATVKVMGGEDWQLWIGALSEA
                     GVLAPGCVTLAYSYVGPAHTAGIYRGGSIGRAKEHLERTAAAISDRLAASGGQAFVAV
                     NKAVVTRASAVIPVVSLYVAALFKTMKELGLHEDCQDQMLRLYRERLLAPSAVPTDAA
                     GRIRLDDLEMRADVQEATARRFSAATEENLAETVDLDGFRHDFLEAHGFDMPGVDYDA
                     DVAFR"
BASE COUNT          455 a          915 c         1039 g          527 t
ORIGIN      
        1 ccgagcggct gagcatggtt cccgagcgac tgagcatggt tcccgagcgg ctgagcatgg
       61 ttcccgagcg gctgagcatg gttcccgagc gactgagcat ggttcccgag cggctgagag
      121 agaccatccg gccatcctgg tcatcatgtt ctgatttctt ggcgttctct gcgcatcggc
      181 gcctttacgc gaggcgattc ctaataggtt ccgcgtagag gtgcggaggc gataaggaaa
      241 agggcttcta cgacgtaaag cggaacatga tagtgcagga tttctcaaga tgaccatgat
      301 gatggatagg gaaagaacct tatacaggaa ttcatcatgg tcatcctgct atatcttaaa
      361 caccccaatc catagtcgtt atcttgccca tcctgtcctt atcttgttaa tcatgttctg
      421 tatttttcta ttcctccgtg ccttggtttg aggacgatgc gccgctcggc tcagatgtag
      481 ccgagcttgc cgagggccat ggccacggcg acgacgcagg ctgtctcggt cttgaggatg
      541 cggtcgccga ggctcgcgaa ggggaagccg ctggccgata gcaggtcgag ctcggcgtcc
      601 gtccagccgc gctcgctgcc tatggccagg acgagcggcc gttccggccc ggagtcggac
      661 agggcgaccg agccgaaggg acgctcggcc cggtaggggt cgaaggccca gaaccggctc
      721 gcgccatcgc gcgcagcgtc gtcggcctcc ggaacgatag cgtcgagggc gcgggccagg
      781 gtccaggcgg tctcgacgac cggcagcctc gggttgccgg cctgctcagc cccctcgatg
      841 agcgcggagc ggaactcgcg gttcttgaag aagtcgctct ggaagtacga tttctcgccg
      901 agctcggtgc ccgacagcat gatgcgcgcc acgccgaggg acgcgaggtc cttgaggatg
      961 cggttggcct ggatgggccg cgggaagccg aggagcaggg tgacgggccg caggggcggc
     1021 gcctcggggc cgggggcgaa ggacagcgtc atgccgtcct cgtcgtcggc ctctatccgc
     1081 gcgacgccga gcctgccgtc cgctcgcccg gcagccagct cgtcgccagg gcccttcttg
     1141 agcacggagc gtatatgctt gcggcgcggg tcgccgcgcc cgagagcgag gccggcctcg
     1201 ccggcctcga tgaagagcat gttcatggga gcctaggata gccgaaggac tcgagaaaaa
     1261 aaagaccgcc gcgttgctcg atggacggcg ccggaatggg cgcctttcgg gcccgccatc
     1321 gcggaggcgg gccggcgcgc cgatggctga tcggcgcgta tccgttagga atgggtggtc
     1381 gatgcctttc ctgatacctc ctagggtcca ttcgagtaac gcggcggccg gcaggtccgc
     1441 attcgtcccc ggttcgggcg gtcgtctccc cgggggattg tccaatcaaa ttggccatgg
     1501 agacgcagag cgaggttggc ttctaaccaa aacacgtttt ctccgtgcct ccgtgtctcc
     1561 gtgtctccgt ggttagtctt tccttatgtt ggccctatct gaacgccacg tcggcatcgt
     1621 agtcgacgcc gggcatgtcg aagccgtgcg cctcgaggaa gtcgtgcctg aaaccgtcga
     1681 ggtcgacggt ctcggcgagg ttctcctcgg tggccgcgga gaagcggcgg gcggtcgcct
     1741 cctgcacgtc ggcgcgcatc tcgaggtcgt cgaggcgtat gcggcccgct gcgtcggtcg
     1801 gcacggcgct gggcgcgagg aggcgctcgc ggtacagcct gagcatctgg tcctggcagt
     1861 cctcgtggag gccgagctcc ttcatggtct tgaagagggc ggccacatag agggagacga
     1921 cggggatgac ggcgctggcg cgcgtgacga cggccttgtt cacggccacg aaggcctggc
     1981 cgcccgaggc ggcgagccgg tcggagatgg ccgcggcggt ccgctcgaga tgttccttgg
     2041 cgcggcctat ggaaccgccg cggtagatgc ccgcggtgtg cgccggtccc acgtaggagt
     2101 aggcgagggt gacgcagccg ggggccagga cgccggcctc gctcagggcg ccgatccata
     2161 gctgccagtc ttcgccgccc atcaccttga cagtcgcctc gacctcctcg tcggtggcgg
     2221 gctggatggt cacctcgctc atgcggccgt cgacgacgtc gaaagtgcgg ccggtgaacg
     2281 gttcgccgag gggcttgagc acggaacggt gcatgacgcc ggtcgccggg tcgacgcgta
     2341 cggggctcgc cagggagtag acgacctgat cgaagacgag gccgcactcg cgggccaggg
     2401 cgacgacggc cgcccgggtg gcgtcggcga aggcgtcggc gtcgatggtc gccgagaaga
     2461 gtccatcgcg cgtgcaggcc gcgtcgaagg cgcggttgtc gtaccagccg ggcgtgcccg
     2521 ccttcttgtc gctgggttcg cgttcgagcg acacgccgac ggtggcggcg ccgcaaccga
     2581 aggccgccgc tatcctggaa gccaggccgt agccggtgga gcagccgagt acgagcgtcg
     2641 ccctcggtag cggcctgccg gcggccaagg ccgccgcggt catggccgga gcgtcggcct
     2701 tggcgcgggc tatccagttt tcgaccatcg cggcgcaacc ttcggggtgc gcgttcaggc
     2761 agatattgcc tcgtatcata ggcttcatcg tcgacctccc tcgtcgtccc gcttcgagcc
     2821 agaggccgcc ccggttggcg gttttccggc tatacagagc cattcggcct cgaccgcgag
     2881 ctcgccgtcc acgctgcccg cgccggactg cttgatgatc ttgcctgagt cgcgta
//
LOCUS       DAOOLN010000093         2933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_41258_length_2933_cov_3.508687, whole genome shotgun sequence.
ACCESSION   DAOOLN010000093 DAOOLN010000000
VERSION     DAOOLN010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41258_length_2933_cov_3.508687"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PLW80_01845"
     CDS             complement(<1..556)
                     /locus_tag="PLW80_01845"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPB65270.1"
                     /translation="MLLALDARNSGIVAGFRDGDGWKSIVRLGVDRSADEIGILLEAA
                     AARAGVGTTTASVDEAWLSCVVPALTSRLVHAVREAFGIDASTVGPGTRTGLKIRTDN
                     PSELGSDIVCSCVAARCVAARCVAAREITSREAESPAVIVVDFEAALVVSAVNADGEL
                     LGVSIAPGLETAARSLRASAAQIPE"
     gene            complement(560..1168)
                     /locus_tag="PLW80_01850"
     CDS             complement(560..1168)
                     /locus_tag="PLW80_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPB65271.1"
                     /translation="MERSNVRKIVVAGALGAVSAVMAVTPLGYLPWFGGASLTIMHIP
                     VIIAAVLEGPLAGTAVGLVFGVTSLVRAATAPVGPLDPLFANPIVSVLPRLFIAIAAW
                     AAFKLFRGKASPVAAGFAGAVGSLVNTALVLSALGLVGAEQLSGLFGVEPGALPAALA
                     ALALSNGLVEAAAAAVFTSSVVSAWKGIESARGKARLSEEER"
     gene            complement(1258..2106)
                     /locus_tag="PLW80_01855"
     CDS             complement(1258..2106)
                     /locus_tag="PLW80_01855"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HPB65272.1"
                     /translation="MKGLEFWKHVSIGRYVESDSMVHRMSPSTKYLWLLAAVLPASVS
                     RSAIVVAAVTLLPLILAPLAGVRIRFLLRGVLPVLPFVALAAAIQLLIAWPNDASPVL
                     ARVGPVSVTAREALAVLTMGLRFIAIMTAIGLFTSVTSEGDTARGVEDLFSPLSSLGF
                     PARPLALMIAVAFRFIPIIAGEFESVVKAQAARGADFGAGARGPIRKAKAYLPLLVPV
                     TIRALERAEALAEAMEARCYGTGPATRYVDTAGGAADVVARIVAMLWILAALAGPALA
                     ASVGGS"
     gene            complement(2103..>2933)
                     /locus_tag="PLW80_01860"
     CDS             complement(2103..>2933)
                     /locus_tag="PLW80_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB65273.1"
                     /translation="DSAATGSAATGSGGASAFSLRGASMRYLAGTESERLAVDAATLE
                     LPSGAITALIGRTGSGKSSLLQLLDALALPDSGIVVSLGRVTAESGADLRAVRMRAPL
                     AVQRPESALFELYAGDEVAFGPRNQGLSGKALVERCRAAMDEAGLPFDEYRDALSRAL
                     SGGRKRRLAIASVLSLDPEALLLDEPTSALDPASRDEVMRLIRSYADRGRTVVMSTHS
                     MDEAARADMVAVMRGGAVVAFGPPGQVFGEGWDPAWGVGRPFAYELLEAVSRDGEATQ
                     "
BASE COUNT          500 a         1050 c         1014 g          369 t
ORIGIN      
        1 tctcggggat ctgggcggcc gaggcgcgga gcgaacgggc ggccgtctcg aggccgggag
       61 cgatcgatac gccgagtagc tcgccgtcgg cgttgaccgc cgacacgacg agggccgcct
      121 cgaagtcgac gacgatgacc gcgggcgact ccgcttcgcg cgacgtaatc tcccgtgcgg
      181 ccacgcaccg tgcggccacg caccgtgcgg ccacgcacga gcagactata tcgctaccaa
      241 gttccgaggg gttatcggtg cggatcttga gccccgtcct ggtgcctggc ccgaccgtcg
      301 aggcgtctat cccgaaggcc tcgcggaccg cgtggacgag gcgggaggta agcgccggga
      361 ccacgcacga cagccaggcc tcgtcgacgg aagccgtcgt cgttcctacg ccggcgcggg
      421 ccgcagcggc ctcgagcagt atgcctatct catctgcgct acggtcgacg ccgagtcgca
      481 cgatggactt ccagccgtct ccatccctga acccggcgac gatgccagaa ttcctcgcgt
      541 cgagcgccaa cagcacggct cagcgctcct cttcggacag ccgggccttg ccccgagcgc
      601 tctcgatgcc cttccaggcg ctgaccaccg agctcgtgaa cacggcggcg gcggcggcct
      661 cgacgaggcc gttggatagg gcgagggcgg cgagggcagc gggcagggcg ccaggctcga
      721 cgccgaacag gccggatagc tgttccgcgc cgacaaggcc gagggccgat agcacgaggg
      781 ccgtgttgac gagactaccg accgcgccgg cgaagcccgc ggctaccggc gacgccttgc
      841 ccctgaacag cttgaaggct gcccaggccg ctatcgcgat gaacaggcgc ggcagcaccg
      901 acacgatcgg gttggcgaac aaggggtcga gcgggccgac aggagcggtt gccgcgcgca
      961 cgaggctcgt cacgccaaag accaacccga cggccgtacc ggcgagcggt ccctcgagca
     1021 cggccgcgat gatgaccggg atatgcatga tggtcagcga agccccgccg aaccacggca
     1081 ggtagccgag cggcgtcacc gccatgacag ccgagaccgc gccgagcgcc ccggcgacca
     1141 cgatcttccg tacgttcgat cgttccattc atcttcctcc gttcggggta cgcgccgcct
     1201 cggacggtcg cgtcccatgg ctatgagcgc ggccctggcc gcgcgcgaag aaccgtacta
     1261 ggagcctccg acggaggcgg cgagcgccgg ccccgccaag gcggcgagta tccatagcat
     1321 tgcgacgatg cgggcgacga cgtcggcggc ccctccggcc gtatcgacgt aacgcgtcgc
     1381 cggccccgtg ccgtagcagc gcgcctccat cgcctcggcg agggcctcgg cccgctcgag
     1441 cgcccggatg gtcaccggca cgaggagggg caggtacgcc ttggccttgc gtatcggccc
     1501 ccgagcgccg gcgccgaaat ccgcgcccct ggccgcctgc gccttgacga ccgactcgaa
     1561 ttcaccggct atgatcggta tgaacctgaa ggccacggcg atcatcagcg cgagcggcct
     1621 ggccgggaag ccgagggatg ataacgggga aaacaggtcc tcgacgccgc gcgccgtatc
     1681 cccctcgctg gtcaccgagg tgaacaggcc gatcgccgtc atgatcgcga tgaagcgcag
     1741 gcccatcgtc agcacagcca gggcctcccg cgccgtgacc gataccggcc ctacccgcgc
     1801 cagcacgggg ctggcgtcgt tcggccaggc gatgagcagc tggatggcag cggccagcgc
     1861 gacgaagggc agcacgggca agacgccgcg caagaggaag cgtatccgga ccccggcaag
     1921 cggcgccagg atcaacggta gcagcgtcac cgcggcgacg acgatcgccg agcgggatac
     1981 gctcgccgga agcaccgccg ccaagagcca caggtacttc gtcgacggcg acatgcgatg
     2041 gaccatcgag tccgactcga cgtagcggcc gatggatacg tgcttccaga attccaggcc
     2101 cttcattgag tagcctcccc atcccgcgag acggcctcga gcaactcgta ggcgaagggg
     2161 cgcccgacgc cccaggccgg atcccagcct tcgccgaata cctgcccagg cggcccgaac
     2221 gcgacgacgg cgccgccccg catcaccgcg accatgtcgg cgcgtgccgc ctcgtccatc
     2281 gaatgagtag acatgacgac ggtccggccg cgatcggcgt aggaccgtat cagacgcatc
     2341 acctcgtctc gcgacgccgg gtcgagggcc gaggtcggct cgtcgaggag tagcgcctcg
     2401 gggtcgagcg acagcaccga cgctatcgcc aggcgacgct tgcggccgcc cgacagggcg
     2461 cgcgacagcg cgtcccgata ctcgtcgaac ggcaggcccg cttcgtccat cgctgccctg
     2521 caacgttcta ccagggcttt gccggacagc ccctggttgc ggggcccgaa ggccacctcg
     2581 tcgccggcgt agagctcgaa cagggcgctc tcggggcgct ggacggcgag cggcgcgcgc
     2641 atacgcacgg cccgcaggtc ggcaccggac tccgcggtca cccggccgag cgatacgacg
     2701 atgcccgagt cgggcagggc caacgcgtcg aggagctgga gcaggctcga tttgcccgaa
     2761 ccggttcgac cgatgagcgc ggtgatcgcc ccggacggca gctcgagcgt cgcggcgtcg
     2821 acggcgagcc gttcgctctc ggtgcccgcc aggtagcgca tcgacgcgcc ccggagcgag
     2881 aaagccgaag cgccgcccga gcccgtggct gccgagcccg tggctgccga gtc
//
LOCUS       DAOOLN010000094         2929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_41335_length_2929_cov_3.872303, whole genome shotgun sequence.
ACCESSION   DAOOLN010000094 DAOOLN010000000
VERSION     DAOOLN010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41335_length_2929_cov_3.872303"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PLW80_01865"
     CDS             <1..304
                     /locus_tag="PLW80_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65274.1"
                     /translation="QAPFFALFLAAIASDKVEGLATLKALSALDLAPLAVLLPLPARA
                     LAWPFPQYWAARLALGASLGPGTGVAVGSPEALGLGLAVSAAWLSFLYARYRRRVD"
     gene            471..1901
                     /locus_tag="PLW80_01870"
     CDS             471..1901
                     /locus_tag="PLW80_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPB65275.1"
                     /translation="MNHTLERRPLFGPAAFYKEVLAVALPVMAQQAIQSLVSLIDNFM
                     VAGLGDAKMAGVNVANQVNFVYMVVMMTLSAAGGIYMSQYRGAKDEEGMRQAYRFKAI
                     MTLGVSALYFAVCQVMPSSLVTMMTIGNAAQAEIVAHGASYLRLVSWTFLPMALSQSI
                     GSAFREIGRPNAPLVISVVATGVNTFFNWVMIYGNLGAPALEIRGAAIATIIARVVEL
                     AAFVTYARAVKAPFYVPLAGLLKARWKLFREILGKSSLMLLSETSWVVSETVTTALYN
                     GRGGAEVVAGMSAGWAIANIFFLVFTGIHVSTGVVVGGLLGADRLDEARVKARWIMSG
                     SVVGGTVVALLQVASIAAVPIVFMNLTAEATKVTAGLLVVIAVYLPLWSLLNSQFAVS
                     RAGGDTMMGVVVDVGVTYALFIPGAFALALLTPIGPVYMYAIVKLTDLVKWVVACRWL
                     KKERWLRNLAAPADGAAEKTVVNGAG"
     gene            2171..>2929
                     /locus_tag="PLW80_01875"
     CDS             2171..>2929
                     /locus_tag="PLW80_01875"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HPB65276.1"
                     /translation="MSFPVTSSSASEPGSARPLRKPEWLKVAIPATEGSRMMTRLVGR
                     RGLHTVCDSARCPNKAECWGCGTATFMILGDTCTRNCRFCAVPTGKHGDEPNADEPRQ
                     LAEAIAELGLRYAVLTSVDRDDLPDRGAGHFAACVRAIKAANPGVRVEALTPDYREGE
                     LELILAEGPDALDHNVETVPRLQGVRDARASYAASLHTLELAAAWSPGRPAVVKSSLL
                     LGLGETRDEVLATMDDLRAIGCDAIVLGQYLRPTS"
BASE COUNT          414 a         1004 c         1018 g          493 t
ORIGIN      
        1 ccaggcgccg ttcttcgcgc tgttcctggc cgccatcgcc tccgacaagg tggagggcct
       61 ggctacgctc aaggccttga gcgccctcga cctggcgcct ctcgcggtgt tgctgccgct
      121 gccagcccgg gcgctggcct ggccctttcc gcagtactgg gcggcgcgcc tcgcccttgg
      181 ggcgtcgctc gggccgggaa ccggcgtcgc cgtcggttcg cctgaggcgc tcggacttgg
      241 cctggcggtt agcgcggcct ggctctcgtt cctatatgcg cgctaccgcc gccgggtgga
      301 ctagcggccg cgccgcgcgc ggcgcccggg cgggtggagc cgaccgggag ccgaatggaa
      361 cggcggccgc cttgggcggc cgggcgcggc gcccgccgcg cctgtccgaa gattcctata
      421 gtcgtcctgt ccctattgcc ctttattttt ttcttatcta tacatgagcg atgaaccata
      481 cgcttgaacg ccggccgctc ttcgggccag ccgcgtttta taaagaagtg ctcgccgtcg
      541 cgttgccggt gatggcgcag caggccatcc agagcctcgt gtcgctgata gacaacttca
      601 tggtggcggg gctcggcgac gccaagatgg ccggcgtcaa cgtggccaac caggtcaact
      661 tcgtgtacat ggtcgtgatg atgaccctct cggccgccgg cggcatctac atgtcgcagt
      721 accgcggcgc caaggacgag gaaggcatgc gccaggccta ccgcttcaag gccatcatga
      781 cccttggcgt ctcggcgctg tacttcgccg tctgccaggt catgccttcg agcctggtga
      841 cgatgatgac gatcggtaac gctgcccagg cggagatcgt cgcgcacggc gccagctacc
      901 tacggctcgt ctcgtggacc ttcctaccga tggccctgtc gcagtcgata ggctcggcgt
      961 tcagggagat aggccggccg aacgctccgc tcgtgatctc ggtcgtggct acgggcgtca
     1021 ataccttctt caactgggtg atgatctacg gcaacctggg cgctccggcc ttggagatac
     1081 gcggtgccgc catcgcgacc atcatcgcgc gcgtcgtcga gctggcggcc ttcgtgacct
     1141 atgcccgagc ggtcaaggcg ccgttctacg taccgctcgc gggcctcttg aaggcgcgct
     1201 ggaagctgtt ccgcgagatc ctgggcaagt ccagcctgat gctcctgtcc gagacgagct
     1261 gggtggtatc ggagaccgtg acgacggccc tgtacaacgg ccgtggcggc gccgaggtgg
     1321 tggccggcat gtcggccggc tgggcgatcg cgaacatctt cttcctcgtg ttcaccggca
     1381 tccacgtgtc cacgggagtc gtcgtcggcg gcctcctggg cgccgatagg ctcgatgagg
     1441 cgagggtgaa ggcgcgctgg atcatgagcg gctcggtggt cggaggcacc gtcgtggcgc
     1501 tcctgcaggt ggcgagcatc gccgccgtgc cgatcgtgtt catgaatctg acggccgagg
     1561 cgaccaaggt gaccgccggc ctcctcgtcg tcatcgcggt ctacctgccg ctctggagcc
     1621 tcctcaactc ccagttcgcc gtgtcgcggg ccggcggcga tacgatgatg ggcgtggtcg
     1681 tcgacgtcgg cgtgacctac gcgctgttca tcccgggcgc cttcgcgctg gccctgctga
     1741 cgccgatagg gcccgtgtac atgtacgcga tcgtcaagct gaccgacctg gtcaagtggg
     1801 tcgtggcatg ccgctggctc aagaaggagc gctggctcag gaacctcgcg gcgccggcgg
     1861 acggagcggc ggagaagacc gtcgtgaacg gcgccggctg agcggcggag aaggccgtcg
     1921 tgaacggcgc cggctgagcc gtcggccgag ccgccggccg agtggcgggg aaggccgtcg
     1981 tgaacggcgc cggctgagcc gtcggccgag ccgccggccg agcggcggag aagaccgtcg
     2041 tgaacggcgc cggctgagcg gcgggcatac ataggcttcg taccacagct cgaatagacg
     2101 cccccgcggc ttttacgcta cgatggctgc cgatgggggc gccgcgcgcg gccccgggag
     2161 ggtcgtagcc ttgtcatttc ccgtaacgag tagttccgcc tcggagcccg gttcggcccg
     2221 gccgctccgc aagcccgagt ggctcaaggt agccataccc gctaccgagg ggtcgcgcat
     2281 gatgacgcgg ctcgtcggcc gccgcgggct ccataccgtc tgcgattccg cccgctgccc
     2341 gaacaaggcc gagtgctggg gctgcggcac cgccaccttc atgatactcg gcgatacctg
     2401 caccaggaac tgccgcttct gcgccgtgcc cacgggcaag catggcgacg aaccgaacgc
     2461 cgacgagccg cgccagctcg cggaggccat agccgagctc ggcctgcgct acgccgtgct
     2521 gacaagcgtc gaccgggacg acctgccgga ccgcggcgcc ggccacttcg ccgcctgcgt
     2581 acgcgctatc aaggcggcga atcccggcgt ccgcgtcgag gcgctgaccc ccgactaccg
     2641 cgagggcgaa ctcgagctga tcctggccga gggcccggac gcgctcgacc ataacgtcga
     2701 gaccgtgccg cgcctgcagg gcgtgcgcga cgccagggct agctacgccg cgtcgctcca
     2761 taccctggag ctggcggccg cgtggtcccc gggccggccg gccgtcgtca agtccagcct
     2821 gctcctcggc ctcggcgaga cccgcgacga ggtgctcgcc acgatggacg acctgcgcgc
     2881 gatcggctgc gacgccatcg tgctcgggca gtaccttcgg ccgacgagc
//
LOCUS       DAOOLN010000095         2926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_41423_length_2926_cov_3.012887, whole genome shotgun sequence.
ACCESSION   DAOOLN010000095 DAOOLN010000000
VERSION     DAOOLN010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41423_length_2926_cov_3.012887"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PLW80_01880"
     CDS             complement(<1..556)
                     /locus_tag="PLW80_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65277.1"
                     /translation="MAAHGFGSGSRTTMGRAGRAMLGAAVAAILSASATGCWTPTRRP
                     AESVFPLPAFERYEPAAVSEVQRLIADGGRTLAGRKALIVHGVSYPLDCTGLVRAAYG
                     FASIDLAYRFDDYAGNGVRRLYQTLRDEGLLYAVRYPAPADLVFWDDTYDADGDGRPG
                     DELTHVGVVLSVETDGSVEYLHYHY"
     gene            complement(559..1143)
                     /locus_tag="PLW80_01885"
     CDS             complement(559..1143)
                     /locus_tag="PLW80_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPB65278.1"
                     /translation="MTTQSAASDPRAILLEALDDYRSARPEERDTCDSFIAFVASRED
                     CLLRSCLAGHVTGSAFVVDPDLRMTLLVHHAKLDKWLQPGGHCEPGEGAREAAARETL
                     EETGVLAGQLGAGVFDIDVHPIPARGSEPAHYHYDVRYLLVAEPGPTTASDESRAVEW
                     VDLDEAERRNPEPSITRMAAKARALRGVAPRSVG"
     gene            complement(1140..2072)
                     /locus_tag="PLW80_01890"
     CDS             complement(1140..2072)
                     /locus_tag="PLW80_01890"
                     /inference="COORDINATES: protein motif:HMM:NF015763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65279.1"
                     /translation="MATMMRMGRPAVRAATLACAMALAAAPLLASPFTSSDDDAPPLP
                     TVRAPGSQGPMVETQRGLRERAAESIRLFAEEPSGRALAGLLAAAFVYGVLHAMGPGH
                     RKTIVFSLFIGKKAATWEPLAAGFMAAGVHAGVGIAIVGVLSLAYGAIAGLGDAERVS
                     AYLDASTFGVLILAAIVLAALKLRAMASGKGRGSEPSGGRRGLYGIVLVSSLIPCPGA
                     TMILLFALYAGLPWIGAAAVLAMSVGMGVVISAAGYLAYAGRVGLFGSLKAREKTIGL
                     VSDLLELVSYLLVLGFSLYMAWPTITALPEVLGL"
     gene            complement(2072..2716)
                     /locus_tag="PLW80_01895"
     CDS             complement(2072..2716)
                     /locus_tag="PLW80_01895"
                     /inference="COORDINATES: protein motif:HMM:NF017985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HPB65280.1"
                     /translation="MNYRRAVASMLFALCAAPALWAHPHVFIDSSLEFEMSGRDCVGI
                     VVSWTFDVVFSADIIGQFDKDRDGAFNEAETATVQAKAFSNLRKYGYFTFVRKGSVRT
                     CPEAVEAFGVSQSDGRVTYRFRVPLEGKGVSDDFSVATFDSTFYCATKYVDGAASVRW
                     IGDRPADEPTIAIAANKDYPVYYNPLGSATDSRVYTKWEKGLQTAYPEEIRVRF"
BASE COUNT          471 a          972 c         1024 g          459 t
ORIGIN      
        1 ggtagtggta gtgcaggtac tcgacgctcc cgtcggtctc gacggacagt acgacgccga
       61 catgggtcag ttcgtcgccg gggcggccgt cgccgtcggc gtcgtaggtg tcgtcccaga
      121 acacgaggtc ggccggcgcg ggatagcgta ccgcgtagag tagcccctcg tcgcgtagcg
      181 tctggtacag cctgcggacg ccgttgcccg cgtagtcgtc gaagcggtag gccaggtcaa
      241 tcgacgcgaa gccatacgcg gcgcggacga gtccggtaca gtcgaggggg taactgactc
      301 catggacgat gagggccttc ctgccggcga gcgtacgccc gccgtcggct atcagccgct
      361 ggacctccga aacggcggcc ggctcgtagc gctcgaacgc gggcaacggg aagacactct
      421 cggcgggccg cctcgtcggc gtccagcagc cggtcgccga cgccgagagg atagccgcga
      481 ccgcggcgcc gagcatcgct cggcccgctc ggcccatcgt cgttctggaa ccggaaccga
      541 aaccgtgcgc ggccacggct agcccacgct cctcggagcg acgcctcgga gcgcccgcgc
      601 cttggccgcc attcgcgtta tcgacggctc ggggttgcgg cgctccgcct cgtcgagatc
      661 gacccactcg acggcccgcg actcgtcgct ggccgtcgtc ggccccggct cggctacgag
      721 caggtacctc acgtcgtaat gataatgcgc cggctcgctt ccacgggccg gtatcgggtg
      781 gacgtcgata tcgaagacgc cggcgccgag ctggccggcc aggacgccgg tctcctcgag
      841 ggtctcgcgg gccgccgcct cgcgcgcgcc ctcgcccggc tcgcagtggc ctcccggctg
      901 gagccatttg tccagcttgg cgtgatgcac gagcagggtc atgcgcaggt cggggtcgac
      961 gacgaaggcc gagccggtga cgtgccccgc gaggcaggag cggagcaggc agtcctcgcg
     1021 gctcgcgacg aaggcgatga acgagtcgca ggtatcgcgt tcctcggggc gcgccgaacg
     1081 gtagtcgtcg agggcctcca gcagtatcgc cctcgggtcg gatgcggcag attgcgtcgt
     1141 cataatccca ggacctccgg tagggcggtt atggtcggcc aggccatgta cagcgagaag
     1201 ccgagcacca gcaggtacga taccagttcg agcaggtccg agaccagccc gatcgtcttc
     1261 tcgcgggcct tgaggctgcc gaatagcccg acgcggccgg cgtaggccag gtatccggcc
     1321 gcggagatta ccacgcccat gccgaccgac atcgcgagca cggcggcggc gcctatccac
     1381 ggcaggccgg cgtacagcgc gaacagcagt atcatcgtgg cgccggggca gggtatcagg
     1441 ctggagacga ggacgatgcc gtacagccct cgccgcccgc cagagggctc gctcccgcgc
     1501 cccttgccgc tcgccatcgc gcgcagcttg agggctgcga ggacgatggc ggccaggatc
     1561 aggacgccga aggtcgaggc gtccaggtag gccgagacgc gctcggcgtc tcccaggccg
     1621 gctatcgcgc cgtaggccag cgacaatacc ccgacgatgg cgatgccgac gccggcgtgg
     1681 acgccggcgg ccatgaagcc cgccgcgagc ggctcccagg tcgcggcctt cttaccgatg
     1741 aacagcgaga acacgatagt cttgcggtgc cccggcccca tcgcgtgcag gacgccgtag
     1801 acgaaggcgg cggcgagcag gccggcgagg gccctgccgg acggttcctc ggcgaacagc
     1861 cgtatcgact cggccgcgcg ctcgcgcagg ccgcgctgcg tctcgaccat cgggccctgc
     1921 gagccgggcg cgcgcaccgt cggcaacggc ggcgcgtcgt cgtcgctcga cgtgaacggg
     1981 ctcgcgagga gcggggccgc ggcgagcgcc atggcgcagg ccagcgtggc cgccctgacg
     2041 gcgggacggc ccatcctcat catcgtcgcc atcagaacct gacccgtatc tcttcggggt
     2101 aggccgtctg gagccctttc tcccatttcg tgtagacgcg ggagtcggtg gcgctgccga
     2161 gcggattgta gtagaccggg tagtccttgt tggcggctat cgcgatggtc ggttcgtcgg
     2221 ccggccggtc gcctatccag cggacgctcg ccgcgccgtc gacgtacttg gtcgcgcagt
     2281 agaaggtcga gtcgaaggtg gcgacgctga agtcgtccga gacgcccttg ccctcgagcg
     2341 ggaccctgaa gcggtaggtc acgcggccgt cgctctggct gaccccgaag gcctcgaccg
     2401 cttccgggca ggtcctgaca gaacccttgc gcacgaaggt gaagtagccg tacttgcgga
     2461 ggttcgagaa cgccttggcc tgcaccgttg cggtctcggc ctcgttgaag gcgccgtcgc
     2521 ggtccttgtc gaactgccct atgatgtcgg cgctgaagac gacgtcgaag gtccatgata
     2581 ccactatccc gacgcagtcc cggccgctca tctcgaactc gaggctcgag tcgatgaaga
     2641 cgtgcggatg ggcccagagg gctggcgccg cgcatagcgc gaacagcata gaggctacgg
     2701 ctcgtcggta gttcatgggt gctcctcgcc gtcgattcta tagtcaagcg gccggatcgt
     2761 gaagcgttcc gggcgtagaa aatctcggat cccattgtcc ggaaatattg gcatatgcta
     2821 tattggcacg aaataggagc ttgtgccatg aattccgagc gtaacgacga ggatggcggc
     2881 tattcggggc cgatcctgta ttataccgac gccgtcatcg accact
//
LOCUS       DAOOLN010000096         2883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_42257_length_2883_cov_2.958628, whole genome shotgun sequence.
ACCESSION   DAOOLN010000096 DAOOLN010000000
VERSION     DAOOLN010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42257_length_2883_cov_2.958628"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PLW80_01900"
     CDS             complement(<1..187)
                     /locus_tag="PLW80_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HPB65281.1"
                     /translation="MMRSLYSGVAGLQNHQVRMDVLGNNIANVNTTGFKKGRANFQDL
                     LYQQMSGAAKPTEEVGGI"
     gene            complement(200..634)
                     /gene="flgD"
                     /locus_tag="PLW80_01905"
     CDS             complement(200..634)
                     /gene="flgD"
                     /locus_tag="PLW80_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HPB65282.1"
                     /translation="MDIDLRMSQADAARTSYQVDALNKELSGGKAFKTELDKDDFLKI
                     LITQLQHQDPTNPMQDKEFIAQMAQFSSLEQMTNMATSFGKLSTVLNSSEAQSLLGRT
                     VEVLDGDKAIYGKVSQVVRGEFPLVMVNGSFYDLDQVSKVEE"
     gene            complement(650..1912)
                     /locus_tag="PLW80_01910"
     CDS             complement(650..1912)
                     /locus_tag="PLW80_01910"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HPB65283.1"
                     /translation="MLALTRTVTAQSGLGASPPKADSAKAASVQGNPFTRLLVAAERG
                     VARAGTNAAKADGAGASRGVARRAVSAPSGAGGSNAALRAPAPAEASAGAEAARAKAT
                     ATKRSAGDARGITGKHPDEAPVSNVAAFQRRSVAGDEASARDGASALAAIAARDASGA
                     QAAKPTLVPGRDGSDQRRSGGENGSSGIETVGVGAERRADGAKVTVVDLRLKATRGTV
                     SRREAGNEADDRGGLASTAKPAGVADAANPGLALGEPVAETAGSAKQPIEAVVAPTPP
                     TAEGLASRLREGAADIVRSAQIVLRGGDSGVIRLRLEPESLGGVKIELKMAEKQISGK
                     IVVESDIAAEAFRSSLDSLKDAFAECGFETSALEVEVRNGMASGGDGRAGSERDDGPY
                     WSRSLRELDAAVPQLVGSGRDGLLDVVV"
     gene            complement(1974..2561)
                     /locus_tag="PLW80_01915"
     CDS             complement(1974..2561)
                     /locus_tag="PLW80_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlbB"
                     /protein_id="HPB65284.1"
                     /translation="MLIMILALAIGGLFWFDYLGIVDAKAFFAPALRLAGLRTRTGEA
                     LPSDSPTLLDDERFAKQLGAVEAMRQELSERERQAEERQAAVEAMAQEIDDRARELDE
                     REISFKQMAERYENRRANVEQNARYLTGMPPADAVRILAASDDQTVIDVLRAVEELAA
                     KSGEASVVAYWLSLLPAERSATLQRKMNEKPASLD"
     gene            complement(2631..>2883)
                     /locus_tag="PLW80_01920"
     CDS             complement(2631..>2883)
                     /locus_tag="PLW80_01920"
                     /inference="COORDINATES: protein motif:HMM:NF014144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HPB65285.1"
                     /translation="HRAGELYAVRLSQERERLFKSLAAAELEREAARVDYVEATKARR
                     LVTKLREREEAVYYKAVAREETKTMDDLAAGAHARADAR"
BASE COUNT          421 a         1002 c          908 g          552 t
ORIGIN      
        1 tgataccgcc gacctcctcg gtgggcttcg cggctccaga catctgttgg tacaagaggt
       61 cctggaagtt ggcgcggccc ttcttaaagc cggtggtgtt gacattggcg atgttattgc
      121 cgagcacgtc catgcggacc tggtggttct ggaggccggc gacgcctgaa tacaacgagc
      181 gcatcataac ggcgcctcct tactcttcga ctttactgac ctggtcgagg tcgtagaacg
      241 aaccgtttac catgacgagc gggaattcac cgcgcacgac ctgcgacacc ttgccgtaga
      301 tcgccttatc gccgtcgagc acctcgacgg tgcggccgag cagcgactgg gcctcgctgg
      361 agttaagcac ggtcgacagc ttgccgaagc tcgtcgccat gttggtcatc tgctcgagcg
      421 acgagaactg ggccatctgg gcgatgaact ccttatcctg catcgggttg gtcggatcct
      481 ggtgctggag ctgggtgatc aggatcttga ggaagtcgtc cttgtccagc tcggtcttga
      541 aagccttccc gccggacagt tccttgttca gggcgtccac ctggtagctg gtcctggccg
      601 cgtccgcctg gctcatccgt aggtctatat ccatcgatcg ctccttactt tacaccacta
      661 cgtcgaggag gccgtcccgg cccgacccga ccaattgcgg caccgccgcg tcaagttcgc
      721 gcagcgagcg gctccaatag ggaccgtcgt cgcgctcgct cccggcacgc ccatcgccgc
      781 cggaggccat gccgttccgg acctctacct ccagcgcgct cgtctcgaag ccgcattcgg
      841 cgaacgcgtc cttgagcgaa tcgagcgacg atcggaaggc ctcggccgct atatccgatt
      901 ctacgactat cttgccgctg atttgttttt cggccatttt aagctctatc ttgacgcctc
      961 cgagcgattc aggctccagc cggagccgaa tgacccccga atcgcctccg cgcaggacta
     1021 tctgagccga gcggacgata tcagcggctc cctcgcgcaa cctggacgcc aggccctcgg
     1081 ccgtgggagg agtcggagcc acaacggctt caatcggctg cttggcgctt ccagcggttt
     1141 cagcgaccgg ctcgcccaac gcgaggcccg ggttcgcggc atcggctacg cccgccggtt
     1201 tggcggtcga ggcgagcccg ccgcgatcgt cggcctcgtt gccggcctct cgcctcgaga
     1261 cggttcctcg ggtagccttg agccgcaggt ccaccacggt tactttggcc ccgtcagctc
     1321 ttcgctcggc gccgacgccg acggtctcga ttcccgagga accgttctcg ccgcccgagc
     1381 gccgctggtc ggagccatcc cgccccggaa ccagggtggg cttcgcggct tgggcgccgg
     1441 aagcgtcccg cgcggcgata gccgccagcg cggaggctcc gtcgcgcgcc gacgcctcgt
     1501 cacccgcgac ggagcggcgc tgaaaggcgg cgacgttcga gacgggggcc tcgtcgggat
     1561 gcttcccggt tattccgcgc gcatcgccgg cgctccgttt cgtagcggtg gccttggctc
     1621 gcgcggcttc ggctccggcg ctcgcctcgg cgggcgctgg agctctcagg gcggcattcg
     1681 acccgccggc tccggagggg gcggaaacgg cccgccgggc aacgccgcgg gacgcgcctg
     1741 ccccgtcggc tttggccgcg ttggtccctg ccctggcgac gccgcgctcg gccgcgacca
     1801 gcagcctagt gaatggattg ccttgtacgc tcgccgcctt agcggagtcg gccttcgggg
     1861 gcgaggcccc caggccgctc tgggcggtta cggtacgtgt tagcgccagc atgtatgcgg
     1921 gctccttccg cgtccggcta ggccgaaccg ctcgggagga gtcggctcgg gcgctagtcc
     1981 agcgacgctg gtttctcgtt catcttgcgc tgcagcgtcg cggaacgctc cgcgggcaac
     2041 agcgacagcc aatacgccac gacggaggct tctcccgact tggcggccaa ctcctccacc
     2101 gctctcagca cgtcgatgac cgtctgatcg tccgaggccg cgagtatcct cacagcgtcg
     2161 gccggcggca tgccggtcag atacctggcg ttctgctcga cgttcgctct tctattctcg
     2221 taccgttcgg ccatttgttt gaatgaaatc tcgcgctcgt cgagttccct ggccctatcg
     2281 tcgatttcct gagccatagc ctcgaccgcg gcctgcctct cctcggcctg acgctcgcgc
     2341 tcggatagct cctggcgcat cgcttcgacg gcgccgagct gcttcgcgaa gcgctcgtcg
     2401 tcgagcagcg tcggggaatc ggatggtagc gcctcgccgg tacgcgtcct gaggccggcc
     2461 agcctcaggg ccggggcgaa gaaagccttg gcgtccacga tcccgaggta atcgaaccag
     2521 aacaagccac ctatggcgag ggcgaggatc atgatgagca tgacgactac cctaccgaaa
     2581 acgcgcctct taccgaacga tgccatactg cccctctacg ttccagatgc ctagcgtgcg
     2641 tcggcacggg cgtgcgcccc ggcggccagg tcgtccatcg tcttcgtctc ttcgcgggcg
     2701 accgccttat agtacacggc ttcttctcgc tccctcaact tggtgacgag gcggcgggcc
     2761 ttggtagcct cgacgtagtc gacgcgcgcc gcctcgcgct cgagctcggc ggccgccaac
     2821 gacttgaaca gccgctcgcg ctcctgcgac agcctgaccg cgtacagctc gccggcccgg
     2881 tgg
//
LOCUS       DAOOLN010000097         2883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_42258_length_2883_cov_2.916549, whole genome shotgun sequence.
ACCESSION   DAOOLN010000097 DAOOLN010000000
VERSION     DAOOLN010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42258_length_2883_cov_2.916549"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..1501
                     /locus_tag="PLW80_01925"
     CDS             152..1501
                     /locus_tag="PLW80_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPB65286.1"
                     /translation="MRLSPLYRTVLAIAAPSVVELLLTSLTHLVDTIMVGRLGAYAIS
                     AVGLTTQPKFIMLAMFVALNVGSTALVARFKGQGDRASAELVSAQSVILALLAAGALT
                     VPGILLARPMVLFMGAAPDTVEAATSYFRILMMGFVPTTLPIAITALLRGVGDAKTSM
                     QYNIVANVVNVIFNYLLIYGKFGFPALGVAGAAIATVIGNCAACAMAVYALFRRRSGE
                     RSFASFGLTRENLVPNPAMLKRIVRIGLPSAAEQLALRAGLVIYTITITALGTEVFAA
                     HQIVLSILNISFVNGQAFGIAATSLAGQALGRGSPDAARRASGACQKLGAVVSTVMGV
                     LMFVFRRSLVAMFTAEPAIIAISSGVMLLVAFIQPFQSSFQIYAGALRGAGDSLYPAI
                     SLAAGILIIRPSLSYAFIHLLGLGLFGAWLALLADQVVRFGLIAIRFRGGKWVHTRV"
     gene            complement(1513..2049)
                     /locus_tag="PLW80_01930"
     CDS             complement(1513..2049)
                     /locus_tag="PLW80_01930"
                     /inference="COORDINATES: protein motif:HMM:NF015318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON domain-containing protein"
                     /protein_id="HPB65287.1"
                     /translation="MKRVVFVMVALMAAASAASAQDLVRSSAAPSIDGIAAAGEYSTW
                     FDRSEIRVGLSLSADGSTLYVAVDAPTAGWVAVGLGSLKMDGAYMVLAYVEDSGTVFI
                     GEETGYGRSHRPNAEKRLRASAVREANGRTTLELALPSAGLADGASLKALFAYGRRDD
                     RITKHVRYITAELPIVRR"
     gene            complement(2046..2696)
                     /locus_tag="PLW80_01935"
     CDS             complement(2046..2696)
                     /locus_tag="PLW80_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65288.1"
                     /translation="MKVTKSLVFIAWFLLPAIPAALYAASVGGFPDAYSVSVALGAYA
                     YALLAAQPALAARPAFIVKAIGAKGSATLHGVAPVLALGLAAAHRALKLGLGFEAGTT
                     QAVIGAASFWFLVAASIAAVILLAAFGDALGKALKPLRAWVAERLGLNYKAARAAHGL
                     AALAVVALFVHLSLASSSDLSFNPAGPAWLAAWTLVSFGVYARSRLSARRAGKASR"
BASE COUNT          438 a         1013 c          966 g          466 t
ORIGIN      
        1 ggctcaggct gcgcgcgatg aggacctatc gggaagccgt gccgctcgcc tcggtcgagg
       61 cggcccacgc cgcgatcgtg gccgaggcta accgcgccta agcgcccgct tgccgcgccg
      121 agcgcttcgg ctatatagtc aagcattgtc tatgcgcctt tcccccctgt accggaccgt
      181 cctggctatc gccgctccgt ctgtagtcga gttgctcctc acctcgctga cccacctggt
      241 cgataccatc atggtgggcc gcctcggcgc ctacgccatt tcggccgtcg gcctgaccac
      301 gcagcccaag ttcatcatgc tcgccatgtt cgtggcgctc aacgtcggct cgacggcgct
      361 cgtcgccagg ttcaagggcc agggcgaccg cgcgtcggcg gagctggtct cggcccagtc
      421 ggtgatactg gcgctgctgg ccgccggcgc gctgacggtg ccaggcatcc tcctggccag
      481 gccgatggtg ctgttcatgg gcgcggcgcc cgacaccgtc gaggcggcca cgagctattt
      541 caggatactg atgatgggtt tcgtgccgac gacgctcccc atagcgatca ccgcgctgct
      601 gcgcggcgtc ggcgacgcca agacctcgat gcagtacaat atcgtcgcca acgtcgtcaa
      661 cgtgatcttc aattacctgt tgatctacgg caaattcggt ttcccggccc tcggcgtggc
      721 gggcgcggcg atagccacgg tgataggcaa ctgcgccgcc tgcgcgatgg ccgtctacgc
      781 gctgttccgc cggcgctcag gcgagcgcag cttcgcgtcc ttcggcctga cccgggagaa
      841 cctggtgccg aacccggcga tgctcaagcg catcgtcagg ataggcctgc cttcggccgc
      901 cgagcagctg gccctgcgcg cggggctcgt catctatacg atcaccatca ccgcgctcgg
      961 taccgaggtc ttcgccgccc atcagatcgt gctgtcgatc ctgaatatat ccttcgtcaa
     1021 cggccaggcc ttcggcatag cggcgaccag cctggccggc caggcgctcg ggcggggcag
     1081 cccggacgcc gcgcggcggg cctcgggcgc ttgccagaag ctgggcgccg tcgtgtcgac
     1141 cgtcatgggc gtcctgatgt tcgtcttcag gcgctccctc gtcgcgatgt tcaccgccga
     1201 gccggcgatc atcgccatca gctccggcgt gatgctgctc gtcgccttca tccagccctt
     1261 ccagtcgtcg ttccagatct acgccggcgc cctccgcggg gccggcgact cgctctatcc
     1321 ggccatatcg ctggccgccg gcatcctgat catcaggccc tccctgtcct acgccttcat
     1381 ccacctcctc ggcctcggcc tgttcggggc ctggctggcc ctgctcgccg accaggtggt
     1441 acggttcgga ttgatcgcta tacgcttccg aggcggcaag tgggtgcaca ccagggtcta
     1501 gccggcgcgc tcctagcggc ggacgatggg caactccgcc gtgatgtagc ggacgtgctt
     1561 ggtgatccgg tcgtccctgc ggccgtaggc gaacagcgcc ttcaggctcg cgccgtcggc
     1621 gaggcccgcg gagggcaggg cgagttcgag ggtcgtgcgg ccgttcgcct cgcgtaccgc
     1681 cgaggctcgc agccgcttct ccgcgttcgg ccggtgcgag cgcccgtagc ccgtctcctc
     1741 gccgatgaag accgtgcccg agtcctcgac gtaggccagc accatgtagg cgccgtccat
     1801 cttgagcgac ccgaggccga cggctaccca gccggcggtc ggcgcgtcga cggcgacgta
     1861 gagcgtcgag ccgtcggccg atagcgacag gcccacccgt atctccgagc gatcgaacca
     1921 ggtggagtac tcgcccgcgg cggctatccc gtcgatggac ggcgcggcgg acgaccggac
     1981 caagtcctgg gccgaggcgg ccgacgccgc ggccatcaac gctaccatga caaaaacgac
     2041 gcgcttcatc gcgatgcctt tcccgcgcgc ctcgccgaca ggcgcgaacg cgcgtatacg
     2101 ccgaacgata cgagagtcca ggcggccagc caggccggcc cggccgggtt gaacgatagg
     2161 tcggagctcg aggcgagcga caggtggacg aacagggcga cgacggccag cgcggccagg
     2221 ccatgagcgg ccctcgcggc cttgtagttc aggccgagcc gctcggctac ccaggccctg
     2281 agcggcttga gcgccttgcc gagggcgtcg ccgaaggcgg cgagcaggat aacggccgcg
     2341 atcgaggccg cgactaggaa ccagaacgag gccgcgccga tgacggcctg ggtcgtaccg
     2401 gcctcgaagc cgaggcccag cttgagcgcc cggtgggcgg cggccaggcc gagggccagg
     2461 accggagcga cgccgtgcag ggtggcgctt ccctttgcgc cgatagcctt gacgatgaag
     2521 gccggcctgg cggccagggc cggctgagcg gccagaagcg cgtaggcgta ggcgcccagg
     2581 gcgaccgaga cgctataggc gtccgggaat ccgccgaccg aggccgcgta cagcgcggct
     2641 ggtatggcgg gcaagaggaa ccacgcgatg aacacgagcg attttgtcac cttcattacc
     2701 tccctagtgc gaaggatacc gcttcggtag cccgcgggca aacgaagccc gtaaacgaag
     2761 ccggaaggcc gggctaggcg cccccgctat cgagctcgcg gtactcgatg ccgaactcgt
     2821 agtcccagtc gccgaagtac agattgccgc cgttccactc gacctcgccg cgctggaagt
     2881 ccg
//
LOCUS       DAOOLN010000098         2882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_42272_length_2882_cov_3.423417, whole genome shotgun sequence.
ACCESSION   DAOOLN010000098 DAOOLN010000000
VERSION     DAOOLN010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42272_length_2882_cov_3.423417"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..2487
                     /locus_tag="PLW80_01940"
     CDS             91..2487
                     /locus_tag="PLW80_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65289.1"
                     /translation="MGSVPAASAILAPLLAFLAARWLALLVGKRYPNGRVGTMAALGV
                     VSSVALSAIPTIGRLEAGAYWLAADAASVGAMGALGATLGARLGVVAKAPGRAVPSDE
                     PAAWRRGLALALPLGVGFAIAAVFGLLTGADGQAVARAAVAFAATLWLAVDARPPALA
                     LAGVALVFAQFRSPAITRPPIQPVVAAIATIAAIAAAAATYKARPARDTAPALASSSP
                     SAQAAEAFQAFAAVALAWSLTAVGLGWPAAGLVAGAVGSLAGRRARHASSASGTGRFK
                     IESAMALAFIAGYGVSAPKAAANLPAALVIAASILVATALVSVLARNGLSRVPVAAGA
                     FAAMLVARRSGLASDAAMAGLVLAYLVAIPVSARASARPGTDDTARRIGTIVGVRPGA
                     DNLAAMALGAALDGRDRPVMAACVAAPEGSPGLGVDQAEETLVRCVAAAASTDVRVVP
                     TLVASRTVAAGLAGLASERGAELLLVGFDDEQKPSGTVDGLLASFPGAVIAVKRPGEF
                     QAAKRLVALAVSGAEASPGFGRAIAAIARAWAGDPKSVEAVMIGAPASAMLEAAEGLL
                     RPSRASSVASWRDVPAAIGTPTSDEAAFVVFSSRPGAKDWNPGHERLPVVMSSSFPDS
                     PTLLWFPPTASDDADKGDDAPAPALPTEPVAASEPYDRLPTIVQAAYAAGRVFVDMGE
                     DSLVDAIRRLTDSIFPFDRVASGRLAAQFSTVARKEPIELAPGILLLHAHAPGVSQTT
                     LAIGSRPDGWPIIALSSPVRVAVALVSPDDSGPEAHLDALSEVATAFRNLGLAELLQA
                     "
     gene            2590..>2882
                     /locus_tag="PLW80_01945"
     CDS             2590..>2882
                     /locus_tag="PLW80_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HPB65290.1"
                     /translation="MKDFADILARPHTAETIERLVRYAKIETTSDRHVDVIPSTPSQW
                     NLARLLETEIKALGVADVSVDEHCFVIARIPATPGCEKKPTIGLMAHLDTSSEV"
BASE COUNT          369 a         1060 c          994 g          459 t
ORIGIN      
        1 ggtggtaacg ctcggcgact tctagggccg acccgcgtcc gatcgaccct tgcgccgggg
       61 cgagggatcg cctagtttta gtcctacgat atgggtagcg tcccggcggc ctcggctata
      121 ctagcgcctc tcctggcctt cctggccgcg cgctggctcg cgctcctcgt cggcaagcga
      181 tacccgaatg gacgggtcgg gacgatggcg gcgctcggcg tggtctcatc ggtggcgctg
      241 tcggccatcc ctacgatagg gaggctcgaa gccggcgcct actggctggc ggccgacgca
      301 gcttccgtag gcgccatggg cgccctggga gcgacgctcg gcgcgcgcct cggcgtggta
      361 gccaaggctc ccggacgcgc cgttccgagt gacgagcccg cggcctggag acgtggcctc
      421 gcgctcgccc tgccccttgg cgtcggattc gcgatagcgg cggtattcgg cctgctaacc
      481 ggtgccgacg gccaggccgt ggccagggcg gccgtcgcct tcgcggcgac gctatggctc
      541 gccgtcgacg ctcggccgcc ggcgctggcg ctcgccggcg tagcgctggt gttcgctcag
      601 ttcaggtcgc cggccataac ccggccccct atccaaccgg tcgtcgcagc tatcgccacg
      661 atagctgcca tagcggcggc cgcggcgacc tacaaggcga ggccggcccg cgatacggcg
      721 ccggctcttg catcctccag cccgtcggcc caggcggccg aggccttcca ggccttcgcc
      781 gcggtggccc tggcttggtc gctcacagcg gtgggactgg gttggccggc ggccggcctc
      841 gtggccgggg cggtcggcag tctggcgggc aggcgcgcgc gacacgctag ttccgcctcc
      901 ggaacggggc gtttcaagat cgagtccgcc atggcgctcg cgttcatagc cggctacggc
      961 gtttccgccc cgaaagccgc ggcgaacctg ccggcggccc tggtcatagc cgcctcgata
     1021 ctcgtcgcga cggccctggt ctcggtcctg gcccggaatg ggctatcgcg tgttccggtg
     1081 gccgccggag ccttcgcggc catgctggtc gccaggcggt ccgggctagc cagcgacgcg
     1141 gcgatggccg gcctggtact ggcgtacctc gtcgccattc ccgtatcggc gcgagcctcg
     1201 gcgcgtccgg ggacagatga taccgctagg cgcatcggga ccatcgtcgg agtgcgcccc
     1261 ggagcggaca acctcgccgc catggccctg ggcgcggccc tcgacgggcg cgatcgcccc
     1321 gtcatggccg cgtgcgtggc cgcgcccgag ggctcgcctg ggcttggcgt cgaccaggcg
     1381 gaggagacgc tcgtccgatg cgtggcggcg gcggcctcga ccgacgtgcg cgtcgttccg
     1441 accctcgtcg cgtcgcgcac cgtcgccgca ggattagccg gcctcgccag cgagcgaggg
     1501 gccgagctcc tgctcgtcgg cttcgatgat gagcagaagc cgtccggcac ggtcgacgga
     1561 ctgctcgcct cgttcccggg cgccgtcatc gcagtcaagc ggccgggcga gttccaggcg
     1621 gccaagcgcc tcgtcgccct ggccgtctcc ggggccgagg cgtctccagg tttcggccgc
     1681 gccatagcgg ccatcgcgcg agcctgggcc ggcgacccca agtccgtcga ggcggtcatg
     1741 atcggcgcgc ctgccagcgc catgctcgag gccgccgagg gcctgcttcg accgtcccga
     1801 gcctcgtcgg tcgcctcgtg gcgcgacgtt ccagcggcta tcggcacgcc gacctccgac
     1861 gaggcggcct tcgtcgtgtt ctcgtcccgc cccggcgcca aggactggaa ccctggacac
     1921 gaacgcctac cagtcgtcat gagctcgtcg ttccccgatt cgccgacgtt gctctggttc
     1981 ccgcccacgg cgagcgacga cgcggacaag ggcgacgatg caccggctcc ggcactaccg
     2041 actgagcccg tggccgcctc tgagccgtac gaccggctgc cgaccatagt ccaggccgcc
     2101 tacgcggccg gcagggtctt cgtcgacatg ggcgaggact cgctggtcga cgctattcgt
     2161 cgcctgaccg actcgatctt cccgttcgac cgggtcgcct cgggcaggct ggccgcccag
     2221 ttctccaccg tcgcgcgcaa ggagccgata gagctggctc ctggcatcct gctcctgcac
     2281 gcccacgcgc cgggcgtctc gcaaacgacc ctggcgatcg gctcgcggcc ggacggctgg
     2341 cccatcatcg ccctgtcgtc cccggtccgc gtcgccgtgg ccctcgtgtc cccggacgac
     2401 tcgggtccgg aggcccacct cgacgcgctc tccgaggtag cgaccgcctt caggaatctc
     2461 ggcctggccg agctcttgca ggcctagcgt cggagggggc ggcgtgtggc ggtgcgcgcc
     2521 tcgtccgctc ccgggagaaa cgcgagcccg gcttgcatag agccgtggct gaaggcatac
     2581 tctcgtggta tgaaagactt cgccgacata cttgccaggc ctcatacggc cgagactatc
     2641 gagcggctcg tgcgctacgc caagatagag accacgagcg atcgtcacgt cgacgtcatc
     2701 ccgtcgactc cgtcccagtg gaacctcgcc aggctcctcg agaccgagat caaggcgctc
     2761 ggcgtcgccg acgtcagcgt cgacgaacac tgcttcgtca ttgcccgcat cccggccacg
     2821 cccggctgcg agaagaagcc gacgataggc ctgatggccc acctggacac ctccagcgag
     2881 gt
//
LOCUS       DAOOLN010000099         2882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_42279_length_2882_cov_2.654404, whole genome shotgun sequence.
ACCESSION   DAOOLN010000099 DAOOLN010000000
VERSION     DAOOLN010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42279_length_2882_cov_2.654404"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /locus_tag="PLW80_01950"
     CDS             complement(<1..1433)
                     /locus_tag="PLW80_01950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPB65291.1"
                     /translation="MNAVIETKPVQGDDGACNQGGPDACCAACAAHERARAEAFSGDA
                     EPRGHGLSAGTRREIATLTVSALLAVAGYVAQAASAESAGGPARWIALALFAVAYLLA
                     GRDVLASAARNIARGKVFDELFLMSIASLGAFAIGAMEEAIGVMIFYKVGEMLQESAA
                     DKSRASIRSLLAMRPDKARVRRGGDWVECRPEDVAPGDLVLVRPGERVPVDGVVTDGA
                     GAFDTSSMTGESAPRPAGPGAEALSGFIAVEAALTIRAERVASESSAARVVELVENAS
                     KAKAKTELFITRFARWYTPAVVAVALLVAFGPPLLVPGQRLADWAYRALVMLVISCPC
                     AFVISVPLGYFGGLGGAARRGILIKGASVLDALADAKTVVFDKTGTLTDGSFSVRELS
                     PEGGFSDEDLLGGAVAAETHSNHPLATAIREAWEGTGKPRPACDDGSYAEIPGHGAVA
                     TVGGREVLAGGDRLLHLRGVPHECRPAT"
     gene            complement(1430..1813)
                     /locus_tag="PLW80_01955"
     CDS             complement(1430..1813)
                     /locus_tag="PLW80_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPB65292.1"
                     /translation="MNPIADKADTECACRSLHEGPIAAARAAEEPVSRLLDLTELFKV
                     LGDPSRLRIIRALGAAELCVCDLSSALEMSQSAVSHQLAILRRSRLVRSRRDGKSVFY
                     ALDDGHVGQIVGIATEHVAERREEP"
     gene            1983..>2882
                     /locus_tag="PLW80_01960"
     CDS             1983..>2882
                     /locus_tag="PLW80_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPB65293.1"
                     /translation="MSLAFSLAIVSAALAAFTVVLAILLRRSAATASAATADASAARA
                     EADARAAEVESLRAVDARLLEELRPVVGAAIAEFRDLLDKAAAVGAKTDSQRETVLAA
                     GDSVSALVRLLSGMVDGLASYAEAFAGSKAAIGALAGGLERMKAEADLLADGAGALLA
                     DSKAGTASLDGAVGAIAEIGEAAKRVRANLGQIAGISARTNLLAMNAAIEAAHAGDSG
                     KGFAVVAGEVRALAELSAKTTKGIEGDMKAMEQAIARGNRAADETKEAFSAIARGVAA
                     SASETGGMAATMDERRRELAAVLP"
BASE COUNT          441 a         1024 c          988 g          429 t
ORIGIN      
        1 gtcgccggcc ggcactcgtg cggcacgcct cgtaggtgca ggaggcggtc tccgccggcg
       61 agcacctcgc ggccgccgac cgtggccacg gcgccgtggc cgggtatctc ggcgtacgag
      121 ccgtcgtcgc aggccgggcg cggcttcccc gtgccctccc aggcctcccg gatggccgtc
      181 gccagcgggt ggttcgagtg cgtctcggcc gcgaccgccc cgccgagcag gtcctcatcg
      241 ctgaagccgc cctctggcga cagctcgcgg acgctgaagc tcccgtcggt cagcgtgccg
      301 gtcttgtcga agactacggt cttggcgtca gccagcgcgt cgagcaccga ggcgcccttg
      361 atcaggatgc cgcgcctggc cgcgccaccc aggccgccga agtagccgag aggcacgctg
      421 atgacgaagg cgcaggggca ggagatgacc agcatcacga gcgctcggta ggcccagtcc
      481 gccaggcgct gccccggtac cagcagcggc ggaccgaacg cgacgagcag ggcgaccgcg
      541 acgaccgccg gcgtgtacca gcgggcgaag cgcgtgatga acagctcggt cttcgccttg
      601 gccttggagg cgttctccac cagctcgacg acgcgcgccg ccgatgactc tgaggctacg
      661 cgctccgccc gtatcgtcag cgcggcctcg accgcgatga agcccgacag cgcctcggcg
      721 cccggacccg ccggcctcgg cgcgctctcg ccggtcatcg acgaggtgtc gaaggcccca
      781 gctccgtcgg tcacgacacc gtcgaccggc acgcgctcgc ctggcctgac cagcaccagg
      841 tcgccgggcg ccacgtcctc gggccggcat tcgacccagt cgcccccgcg cctgacgcgc
      901 gctttgtcgg ggcgcatcgc caggagcgag cgtatcgagg cgcggctctt gtcggcggcg
      961 ctctcctgga gcatctcgcc gactttatag aagatcatga cgcctatcgc ctcctccatg
     1021 gcgccgatgg cgaaggcgcc gagcgaggct atggacatca ggaacagctc gtcgaagacc
     1081 ttgccccggg ctatgttcct ggcggccgat gcgagcacgt cgcggcctgc cagcaggtag
     1141 gctacggcga agagcgcgag ggctatccag cgcgccggac cgcccgcgct ctccgccgag
     1201 gcggcctgcg cgacgtagcc ggcgaccgcc agtagggccg atacggtcag cgtcgctatc
     1261 tcgcgcctcg ttcccgcgga caatccatgt ccacgaggct ccgcgtcgcc cgagaaggcc
     1321 tcggccctgg cccgctcgtg ggccgcgcag gccgcgcagc aggcgtccgg gccgccctgg
     1381 ttgcaggccc catcgtcgcc ctgaaccggc ttcgtctcga tgaccgcatt catggttcct
     1441 cccgcctctc ggccacgtgc tcggtggcga tgccgacgat ctggcccacg tgtccgtcgt
     1501 cgagcgcata gaacaccgac ttgccgtcgc gcctggagcg taccagtctg gagcggcgta
     1561 gtatggccag ctgatggctg accgccgact ggctcatctc caaagccgac gataggtcgc
     1621 agacgcacaa ctcggccgcc ccgagcgcgc gtatgatacg gagccggcta gggtcgccga
     1681 gcaccttgaa cagctcagta agatcgagga gcctggagac cggctcctcg gcggcccggg
     1741 cggcggctat ggggccttcg tgcaacgaac ggcacgcgca ttcggtatct gccttgtcgg
     1801 ctatagggtt catatcagtc tctcctgcta acacatgaac atatgaacaa aggaacatgt
     1861 atatatgtac tgacttggcg tcgccatggc aagcccccgg attgtcccat gacaaacaaa
     1921 acgctaatct gctataattt ccgtaccgtt ccgaagaatc atcgccatcg gagatgccga
     1981 tcatgagtct cgctttttcg ctcgccattg tctcggcggc cctggccgcg ttcacggtcg
     2041 ttctggccat cctcttgcgc cgtagcgccg ccacggcctc cgcggcgacg gccgacgcct
     2101 cggccgcccg cgccgaggcc gacgcccggg cggccgaggt cgagtccctc cgcgccgtcg
     2161 atgccaggct cctcgaggag ctacggccgg tcgtcggcgc ggccatagcc gagttcaggg
     2221 acctcctcga caaggcggcc gcggtcggcg ccaagaccga cagtcagcgt gagacggtgc
     2281 tcgcggccgg cgactcggtg tcggccctgg tcaggctgct gtccggcatg gtcgacggcc
     2341 tcgcctcgta cgcggaggcc tttgccggga gcaaggctgc catcggggct ctcgccggcg
     2401 gtctggagcg gatgaaggct gaggccgacc tcctggcgga cggcgccggc gccctcctcg
     2461 ccgactcgaa ggccggtacc gcctccctcg atggcgccgt tggcgcgatc gccgagatcg
     2521 gggaggcggc caagcgggtc agggcgaacc tcgggcagat tgccggcatc tccgcgcgca
     2581 cgaacctgct ggccatgaac gccgcgatcg aggcggcgca tgcgggcgac tcgggcaagg
     2641 gtttcgcggt cgtggccggc gaggtgcgcg ccctggccga gctgagcgcc aagacgacca
     2701 agggcatcga gggcgacatg aaggccatgg agcaggcgat agcgcgcggc aacagggccg
     2761 ccgacgagac caaggaagcc ttttccgcca tagccagggg tgtggccgcg agcgcctccg
     2821 agaccggcgg tatggccgcg accatggacg agcgcaggcg cgagctggcg gcggtactac
     2881 cg
//
LOCUS       DAOOLN010000100         2860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_42734_length_2860_cov_3.292335, whole genome shotgun sequence.
ACCESSION   DAOOLN010000100 DAOOLN010000000
VERSION     DAOOLN010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42734_length_2860_cov_3.292335"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PLW80_01965"
     CDS             <1..413
                     /locus_tag="PLW80_01965"
                     /inference="COORDINATES: protein motif:HMM:NF015321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HPB65294.1"
                     /translation="VDYDYVVVKIVEAGERYDVRAVMFDPALAQFVDKRLIDEGFVTV
                     QVRQGIAHFSPLAKAWERDVMRGAIADPNPVMAWCVSNAEIKPDVNGNIKPLGDHRSR
                     KIDLVVSSIMAHHGLSIELDDESSVYEERGLLTI"
     gene            410..1630
                     /locus_tag="PLW80_01970"
     CDS             410..1630
                     /locus_tag="PLW80_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HPB65295.1"
                     /translation="MSKLNAMQRLALRIVNRLDRSELSELLLGSPSASGKTVSHESAL
                     AVSTVFACVRILSETIGSLPLHVYKRLERGGKERAYAHPLYDVLATKPNAWQTSMEWR
                     EQMVEHLALRGNYYAAILRHGDDYVDDIIPFHPDRVKVNQLPDYSLIYEVERANGGRL
                     VLLQKDMLHIRAMPGKDGITGRSVIGYQKDVIGSAMATQEYAGRLFRNDATPGVVIKY
                     PKRLDDAAVKRLRDGWNDTYSGSANARKTAILEDGADIAKLSMSAEESQFIETRRLQR
                     SDIAGIFRVPLMLLQADQSTTTYASAEQFMLSFMIHTIRPWLVRIEQSLHRQLFTAQK
                     TYMPEFNPEGMLRGDIATRYKAYATGRQWGWLSANDIRDRENMNPIEGGDLYLTPSNM
                     DRVDDDDEKEKKNA"
     gene            1623..2420
                     /locus_tag="PLW80_01975"
     CDS             1623..2420
                     /locus_tag="PLW80_01975"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HPB65296.1"
                     /translation="MRSKWFAMQALADTAEISIFDEIGGWGITVADFKDAFDLVRDKK
                     AITVYINSPGGDVTTGMALYNLLAHERSKITVEVLGIAASIASVVALAGKELVMDEGS
                     YYMIHNPWSVAWGEADELRKTADVLDKMRGELINLYAARSGLDADEIGQMMDDETWMT
                     ADEAVEKGFASRVHKTVKAAALASFDLSKIGFQHPPKALAPKPRALEIQTIRDYEGFL
                     RDAGFSRAEAEALASGGYKTLRRDAGASRAGVDSIVAALHGVAQSMQ"
     gene            2431..>2860
                     /locus_tag="PLW80_01980"
     CDS             2431..>2860
                     /locus_tag="PLW80_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HPB65297.1"
                     /translation="MDPELKKALDELQSSWASYKKANDERLAALEKGQGVAEIEAKLA
                     RIDADVAKNQKIVDDFSALQAQVNRMNLGTAGGDVGGKSAALKEFNAWAHDKDAGQVF
                     KASATEGVNTDGGFMVVPELEAGIDRVAMDQCVMRQLADVK"
BASE COUNT          584 a          914 c          918 g          444 t
ORIGIN      
        1 acgtcgatta cgactatgtc gtcgtcaaga tcgtggaagc cggagaaagg tacgacgtcc
       61 gcgccgtcat gttcgacccc gcactagcgc agttcgtgga caagcgcctt atcgacgaag
      121 gctttgtcac tgtccaagtt cgccagggca tcgctcattt ctccccgctg gccaaggcgt
      181 gggagcggga tgtcatgcgc ggcgccatcg ctgacccaaa ccccgtcatg gcttggtgtg
      241 taagcaatgc cgagatcaaa ccggatgtca acgggaacat caagccactt ggtgatcacc
      301 gatcaagaaa gatagacctc gtcgtctcgt caatcatggc gcaccacggg ctgtctatcg
      361 agctggacga cgagtcgtcc gtgtacgaag agagggggct tctcacgata tgagcaagtt
      421 gaacgcgatg cagcgcctgg cactcaggat agtcaaccgc ctcgaccgct cagagctgtc
      481 ggagctcctg ctcggatcac cgagcgcgag cggcaagacg gtaagccacg agagcgccct
      541 ggccgtctct acggtcttcg cgtgtgtgcg tatcctctcg gagacgatcg gctccctgcc
      601 cctccacgtc tacaagcggc tcgagcgtgg cggcaaggaa cgggcctacg cgcatccgct
      661 gtacgacgtc ctggccacca agccgaacgc ttggcagacc tcgatggagt ggcgcgagca
      721 gatggtcgag cacctcgcgc tccggggtaa ctactacgcc gcgatcctcc ggcacggcga
      781 cgactatgtc gacgacatca tcccgttcca ccccgaccgc gtgaaggtca accagcttcc
      841 cgactactcg ctcatctacg aagtcgagcg agccaacggg ggccgcctcg tcctgctcca
      901 gaaagacatg ctgcacatcc gtgcgatgcc gggcaaggac gggattacgg gccgctcggt
      961 gatcggctac cagaaggacg tgatcggctc ggccatggcg acgcaggaat acgccggccg
     1021 gctattcagg aacgacgcga cgcccggcgt cgtcatcaag tacccgaagc gactcgacga
     1081 cgcggcggtc aagcgcctcc gcgacggctg gaacgacacg tactcgggat ccgcgaacgc
     1141 caggaagacc gcgatcctcg aggacggcgc cgacattgcc aagctgtcga tgagcgccga
     1201 ggagtcgcag ttcatcgaga ctcggcggct acaacgatcc gacatcgccg ggatcttccg
     1261 tgtgccgctc atgcttctgc aggcagatca gtcgacgacg acgtatgcga gcgcagagca
     1321 gttcatgctt tctttcatga tccacacgat ccggccgtgg ctcgtgcgca tcgagcagtc
     1381 gctgcaccgg cagctgttta ccgcgcagaa gacgtacatg ccggaattca accccgaggg
     1441 catgctccgc ggcgacatcg ccacgcggta caaggcttac gcgacgggcc gccagtgggg
     1501 ctggctgtcg gcgaacgaca tccgcgaccg cgagaacatg aacccgatcg agggcggcga
     1561 cctctacctg acgccgtcga acatggatcg cgtcgacgat gacgacgaga aggagaagaa
     1621 gaatgcgtag caagtggttc gcgatgcagg cgctcgccga cactgccgag atatccatct
     1681 tcgacgagat cggcggctgg ggcataacgg tcgccgactt caaggacgcc ttcgacctag
     1741 tcagggacaa gaaggcgatc accgtgtaca tcaactcccc gggcggcgat gtgacgaccg
     1801 ggatggccct ctacaacctg ctggcgcacg agcgcagcaa gatcaccgtc gaggtcctcg
     1861 gcatcgccgc ctcgatcgcg tcggtcgtcg cgctggccgg taaagagctc gtcatggacg
     1921 agggctcgta ctacatgatc cataacccct ggtccgtggc ctggggcgag gcggacgagc
     1981 tcaggaaaac tgccgacgtg ctcgacaaga tgcgcggcga gctgatcaac ctgtacgcgg
     2041 cccgctcggg tctcgacgcg gacgagatcg gccagatgat ggacgacgag acctggatga
     2101 ccgctgacga agcggtcgag aagggcttcg cgtcgagggt acacaagacg gtcaaggccg
     2161 cggcgctcgc gagcttcgac ctctctaaga tcgggttcca gcacccgccg aaggcgctcg
     2221 cgcctaagcc ccgggcgctc gagatacaga ccatccgcga ctacgagggc ttcctgcggg
     2281 atgcgggatt ctctcgggcc gaggccgaag cgctggcctc gggcggctac aagaccctcc
     2341 ggcgggatgc cggcgcgtcg cgtgccggag tcgacagcat cgtcgcggcc ctgcatgggg
     2401 tcgctcagtc catgcaataa ggagacccgt atggatcccg aactgaagaa agcgctggac
     2461 gagctccagt cgtcctgggc gagctacaag aaggcgaacg acgagcggct cgcggccctc
     2521 gagaagggcc agggcgtcgc cgagatcgag gcgaagctcg cgcggatcga cgccgacgtg
     2581 gccaagaacc agaagatcgt cgacgacttc tcggccctgc aggcgcaggt caaccgcatg
     2641 aacctcggca cggccggcgg cgacgtcggc ggcaagtcgg cggccctcaa ggagttcaac
     2701 gcctgggccc acgacaagga cgccggccag gtcttcaagg cctcggccac cgagggcgtg
     2761 aacaccgacg gcggcttcat ggtcgtcccc gagctcgagg ccggtatcga ccgcgtcgcc
     2821 atggaccagt gcgtcatgcg ccagctcgcc gacgtcaaga
//
LOCUS       DAOOLN010000101         2855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_42833_length_2855_cov_2.873929, whole genome shotgun sequence.
ACCESSION   DAOOLN010000101 DAOOLN010000000
VERSION     DAOOLN010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42833_length_2855_cov_2.873929"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2464
                     /locus_tag="PLW80_01985"
     CDS             <1..2464
                     /locus_tag="PLW80_01985"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB65298.1"
                     /translation="FRDSREWLWVGTSGGGLYRYLPGTDSFLSVRSASSGLPSDYVFS
                     IREDGEGRLWLALWFYGLSRYDPVGGTFENRRLEDGRAYFVNADLDGLVYVGTWGGGL
                     FELSLDDGAIRRFTSDGERRWSLPHDTMYSMLVDRAGDAWVGTNGGGFSMLLHDEAGM
                     SLYEHDPSDPGSLSSGKSISVLEDRRGRLWVGAYNGGVDRLDPGSSSFRRYRHDPDDE
                     RSLPNDIVVKIYEDGRGGLWFLTNDGLARYDEGRDAFERVRRDRSSTEGDAVRILYDM
                     VEEPGTGRYWIATYTSGLELWDPVDGTVERYPSVPGDPSTPSDNLVYAIAYDGRGRLW
                     AATNAGLCRYEGDGRFVRYEASKTDRSKLPSRSLRDVYVDSLGRLWVSTSGGGVARYV
                     EESDSFEHWTKRDGLQSNIVHGIVEAADGSIWAVSTSGLSVFDAAGGRWRPFSDQGRL
                     RYGEFTSGRFASRSGLLYFGAVNALYVIDPAAIAGSPDAPPIRFTSIKSLNEELDTGL
                     SPWFVDRVRLGWSDPHLSLSFAALDYADPTRVQYAYKLEGFDEDWVYSGARSYASYTN
                     LPGGRSYSFKVRAATAIGDWDGEATELFIEVDKAPWLRWWAILLYLVAAGLVAFTVRL
                     YASRAMLKSRVGELSALKGQLESANVKLEALAAHDGLTGLHNRRAFNDELAARWQAAG
                     ATGEPLAALMIDIDFFKPYNDRYGHQAGDACLAAIAAAVKAALDRPHDSASRYGGEEF
                     VALLPGTDAAGAARVAEKVRAAIEALGIPHGGSSVASAVTASVGYASLRPEPGSPPGR
                     LVDLADEALYRAKNGGRNRVSP"
     gene            2514..>2855
                     /locus_tag="PLW80_01990"
     CDS             2514..>2855
                     /locus_tag="PLW80_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002667902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB65299.1"
                     /translation="MASKGSASKYDESKIKTLSSLEHIRLRTGMYIGRLGTGANPDDG
                     IYVLLKEVVDNAVDEFIMGCGDRVTMTVQDGSVSVRDRGRGIPLGKVIECVSVINTGA
                     KYSDDVFQFSVG"
BASE COUNT          455 a          948 c          951 g          501 t
ORIGIN      
        1 gttccgcgac tcgcgggaat ggctctgggt cggcacgtcg ggaggcggcc tgtatcgcta
       61 cctgcccggg accgactcct tcctgtccgt acgctcggcc tcgtccggcc tgccctcgga
      121 ttacgtgttc tcgattcgtg aggatggcga gggccgcctc tggctggcgc tctggttcta
      181 tgggctgtcg cgctacgacc ccgtcggagg gaccttcgag aatcgaaggc tcgaggacgg
      241 ccgggcctac ttcgtcaacg ccgacctcga cggtctggtc tatgtcggta cctggggcgg
      301 cggcctcttc gagctgagct tggacgacgg ggcgatccgt cgattcacca gcgacgggga
      361 gcgccgatgg tcgctccccc atgacacgat gtactcgatg ctcgtcgacc gggccggaga
      421 cgcctgggtc ggtacgaacg gcggcggctt ctcgatgcta ttacacgacg aggccggcat
      481 gtcgctctac gaacacgacc cgtccgaccc cggctcccta tcgtctggca agagcatctc
      541 cgtgctcgag gaccggcggg gacgactctg ggtcggcgcc tataacggcg gcgtagaccg
      601 gctcgatccc ggctcgtcgt ccttcaggcg ctatcgccat gatcccgatg acgagcggag
      661 cctgccgaac gacatcgtcg tcaagatcta cgaggatggc cggggcgggc tctggttcct
      721 gaccaatgac ggcctggcca ggtacgatga ggggcgcgac gcgttcgagc gggtgcgccg
      781 ggatcgttcc tccaccgagg gcgacgccgt ccgtatcctc tatgatatgg ttgaggagcc
      841 cggtacgggc agatactgga tagcaaccta cacctcgggc ctcgagctct gggacccggt
      901 ggacggcacg gtggagcgct atccgtcggt gccgggcgac ccttccaccc cgagcgacaa
      961 ccttgtgtac gcgatcgcct acgacgggcg cggccggctc tgggccgcga cgaacgccgg
     1021 cctgtgccgc tacgagggcg acgggcgctt cgtccgctac gaggcttcga agacggaccg
     1081 ctccaagcta ccgtcccggt cgctacgcga cgtctacgta gactcgctcg ggaggctctg
     1141 ggtatcgaca agcggcggcg gcgtggccag gtacgtcgag gagagcgact cctttgagca
     1201 ctggaccaag cgcgacgggc tccagtcgaa tatcgtacac ggcatcgtcg aggcggctga
     1261 cggatcgatc tgggcggtgt cgacctccgg cctgtcggtg ttcgacgcgg ccggaggccg
     1321 gtggcgcccg ttctccgacc agggccgcct gcgctacggc gagttcacct cgggccgctt
     1381 cgcctcgcgt tccggcctcc tgtacttcgg cgcggtcaac gcgctctatg tcatcgatcc
     1441 ggccgcgatc gccggctcgc cggacgcgcc gcctatcagg ttcacgagca tcaagagcct
     1501 gaacgaggag ctggacaccg gcctatcgcc gtggttcgtc gacagagtca ggctcggctg
     1561 gagcgaccct catctgtcgt tatccttcgc ggccctcgat tacgccgatc cgaccagggt
     1621 ccagtacgcc tacaagctcg agggcttcga tgaggattgg gtctattcgg gcgcgcgctc
     1681 gtacgccagc tataccaacc ttcctggcgg caggtcgtat tcgttcaagg tgcgggccgc
     1741 cacggctatc ggcgactggg acggagaggc gaccgagctc ttcatcgagg tcgacaaggc
     1801 gccgtggcta cgctggtggg ccatccttct gtacctcgtc gcggccggcc tcgtggcctt
     1861 cacggtcagg ctatacgcca gccgggccat gctcaagagt agggtaggcg aactgtctgc
     1921 cctcaagggc cagctcgaga gcgccaacgt caagctcgag gctctggctg cccacgacgg
     1981 cctgaccggc ctccataatc gtagggcctt caacgacgaa ctggccgcgc gctggcaggc
     2041 cgccggggct accggtgaac cgctcgcggc gctgatgata gatatcgact tcttcaagcc
     2101 ctataacgac cgttatggcc atcaggctgg cgacgcctgc ctggcggcca tagccgcggc
     2161 cgtcaaggcc gccctcgacc gtccgcacga ctcggcctcc cgctacggcg gcgaggaatt
     2221 cgtggccctg ctacccggga cggacgcggc cggggcggcc agggtagccg agaaggtcag
     2281 ggctgccatc gaggccctcg gcatcccgca tggcggttcg agcgtcgctt ctgcggtcac
     2341 ggcgagcgtc ggctacgcgt ccctgcggcc ggagcccgga tcgcctccgg gccgcctcgt
     2401 cgatcttgcc gacgaggctc tgtaccgcgc caagaacggc ggacggaacc gcgtctcgcc
     2461 atagcgggct gcgcaacttc tagaagcggg ccgttccgtg ctatagtcga cccatggcat
     2521 cgaaaggctc cgcttcaaag tacgacgaat cgaagataaa gaccttgtcg tcgctcgagc
     2581 acatacggct acgcaccggc atgtacatcg ggcggctcgg caccggcgcg aatcccgacg
     2641 acggcatcta cgtcctcctc aaggaggtcg tggacaacgc cgtcgacgag ttcatcatgg
     2701 gctgcggcga ccgcgtgacc atgaccgtcc aggacggttc ggtgtccgtg cgcgaccgcg
     2761 gacggggcat acccctgggc aaggtgatcg agtgcgtatc cgtaatcaac acgggcgcca
     2821 agtattccga cgacgtgttc cagttctcgg tcggc
//
LOCUS       DAOOLN010000102         2853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_42886_length_2853_cov_2.226233, whole genome shotgun sequence.
ACCESSION   DAOOLN010000102 DAOOLN010000000
VERSION     DAOOLN010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42886_length_2853_cov_2.226233"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..847
                     /locus_tag="PLW80_01995"
     CDS             116..847
                     /locus_tag="PLW80_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB65300.1"
                     /translation="MLGIVELENVRKIYPLGKVQVEAVKGVSFCIEKGDFISIAGPSG
                     SGKTTILNMVGLVDQPTEGEVFLDGKPTSKLNDAELTRLRHETLGFIFQSFNLIPVLN
                     VWENIEFPLLLGKTRVGKDEKKDWIDHLIEEVGLSEWRKHKPNELSGGQRQRVAIARA
                     LVTRPQIVLADEPTANLDSATGEQILELMKKINRELETTFIFSTHDARIVDIADHIIR
                     LRDGLVTENRRREEEAPAACQGAEA"
     gene            850..2370
                     /locus_tag="PLW80_02000"
     CDS             850..2370
                     /locus_tag="PLW80_02000"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPB65301.1"
                     /translation="MPVTLRMAFRNLREHKAKSLIIGILLALGMLIMVLGNSFLDASK
                     RGIERSFTQNYTGDVIITGVADGPVSLFGVQSVGGMEQTPIIPDYEKVFAHVSGLRGV
                     ESMAGMASGYGIATNEAGEIAEDEDEDDEERMEGMMAALMMLFGVDAPGYWELFDTIE
                     LVDGEFIQPGRPGLMMADDRVAKLAEYLKRDIRVGDEILIQGMGDAGIRLRSVPLVGT
                     FKRVTDGAGPEQLTYIDIDTLRALSGMTIGASENIELGEAETSLLALDDLDALFGDDD
                     AFGVEAAPGSGAALTEAGIEGLLGDTSSRERLNAADTGSWHSILLRLDDPARTRAVAA
                     ELNAWFAMEGIQAQAGDWQKAAGPYAQSVDVLRIVFTVAVVILAVVAIIIIMNTLVVS
                     IIERTGEIGTMRALGGGKGFVRRLFAAETVALSLFFGLVGMVLSFLAAAVINAVGFEA
                     SNEFLRILFGGSVLRAVIDPLALLWSLAVIVAVGLVASVYPVSVALRIQPVRAMQS"
     gene            2383..>2853
                     /locus_tag="PLW80_02005"
     CDS             2383..>2853
                     /locus_tag="PLW80_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65302.1"
                     /translation="MNTLANIALRNLTRQKKRTVLLGSAIAFGVMIVTLINGFAGAFS
                     ANVAENFSDLMAGHVFVSGSEKGEGGFFGVSAVWSGQGRVLIDRVTVVSEELFREEEL
                     VRRGGGMELPDEWLLPPATPARIHVVKGLWWDFFGVSEAAAELGGTELTSSYHAQ"
BASE COUNT          501 a          949 c          935 g          468 t
ORIGIN      
        1 gtgccacgcg aacgaacaca acgagttcga cctcgccccg ctcccgcgga aaggcaagct
       61 cgtcgcgttc acgaacctgc acacgctgcc gctcgacttc gatgtgccga cgctcttgct
      121 cggcatcgtg gagcttgaga acgtcaggaa gatctacccg ctcggtaagg tccaggtgga
      181 ggcggtcaag ggcgtatcgt tctgcataga gaagggcgac ttcatatcga tagccgggcc
      241 gtcgggatcc ggcaagacga ccatcctgaa catggtgggc ctcgtagacc agcccaccga
      301 gggcgaggtc ttcctagacg gcaagccgac ctcgaagctg aacgacgcgg agctgacccg
      361 cctgcgccac gagaccctcg gcttcatctt ccagtctttc aacctgatcc cggtgctcaa
      421 cgtctgggag aacatcgagt tcccgctcct cttgggcaag acccgcgtcg gcaaggacga
      481 gaagaaggac tggatcgacc acctgatcga ggaggtcggg ctgtccgagt ggcgcaagca
      541 caagccgaac gagctgtccg gcggccagag gcagcgcgtc gccatcgcgc gggccctggt
      601 taccaggccg cagatcgtgc tggccgacga gccgaccgcc aacctggaca gcgcgaccgg
      661 cgagcagatc ctcgagctca tgaagaagat caaccgcgag ctcgagacta ccttcatctt
      721 cagtacccac gacgcgcgca tcgtcgacat agccgaccac atcatcaggc tacgcgacgg
      781 actcgtgacc gagaaccgcc gccgcgaaga ggaagcgccg gccgcctgcc agggcgccga
      841 ggcctgacca tgcccgtaac gctgcgcatg gcgttccgga acctgaggga gcacaaggcc
      901 aagagcctta tcataggcat actcctggcc ctgggcatgc tcatcatggt tctcggcaac
      961 tccttcctgg acgcctcgaa acggggcata gagagatcgt tcacccagaa ctacacgggc
     1021 gacgtgatca tcaccggcgt cgcggacggc ccggtgtcgc tgttcggcgt gcagtcggtc
     1081 ggcggcatgg agcagacgcc catcatcccg gactacgaga aggtcttcgc ccacgtctcg
     1141 ggcctgcggg gcgtcgagtc gatggccggc atggcctcgg gctacggcat cgccacgaac
     1201 gaggccggcg agatagcgga agacgaggac gaggacgacg aggagcgcat ggagggcatg
     1261 atggccgcct tgatgatgct cttcggcgtc gacgcccccg gctattggga gcttttcgac
     1321 acgatagagc tggtcgacgg cgagttcatc cagcccggcc ggcccggcct catgatggcc
     1381 gacgaccgcg tggccaagct cgccgagtac ctcaagcgcg acatacgggt aggcgacgag
     1441 atactcatcc aggggatggg agacgcgggc atacgcctgc gcagcgtgcc cctcgtcggc
     1501 accttcaagc gcgtgaccga cggcgcgggc ccggaacagc tcacctatat cgacatcgac
     1561 acgctgcgcg cgctgtccgg catgacgata ggcgcttccg agaatatcga gctcggcgag
     1621 gccgagacgt cgctgctcgc gctcgacgac ctcgacgcgc tgttcggcga cgacgacgcc
     1681 ttcggcgtcg aggccgcacc tggctcgggc gccgccctga ccgaggccgg catagaaggc
     1741 ctcctcggcg ataccagctc cagggagcgg ctcaacgcgg ccgacaccgg ctcctggcac
     1801 tcgatactcc tgcgcctcga cgacccggcc cgcaccaggg ccgtggcggc cgagctcaat
     1861 gcctggttcg ccatggaggg catccaggcc caggccggcg actggcagaa ggcggcgggg
     1921 ccgtacgccc agtcggtcga cgtgctgcgc atcgtcttca cggtggccgt cgtcatcctc
     1981 gccgtcgtcg cgatcatcat catcatgaac accctggtcg tctcgatcat cgagcgtacc
     2041 ggcgagattg gcaccatgcg ggcgctcggc ggcggcaagg gcttcgtgcg caggctgttc
     2101 gcggccgaga ccgtggcgct gtcgctgttc ttcggcctcg tcggcatggt cctgtccttt
     2161 ctagcggccg ccgtgatcaa cgccgtcggc ttcgaggcct cgaacgagtt cctgaggatc
     2221 ctgttcggcg gctcggtcct cagggccgtc atcgacccgc tcgcgctcct gtggtcgctc
     2281 gccgtgatcg tggccgtcgg cctcgtcgcg agcgtctacc cggtcagcgt cgcgctgcgc
     2341 atccagcccg tacgggcgat gcaatcttaa ggggataccg ctttgaatac cttggcaaac
     2401 atagccttac ggaacctgac ccgccagaag aagcgcaccg tgctgctcgg cagcgccatc
     2461 gccttcggcg tgatgatagt caccctgatc aacggcttcg ccggagcctt ctcggccaac
     2521 gtcgcggaga acttctccga cctgatggcc ggccacgtct tcgtctccgg ctccgagaag
     2581 ggcgagggcg gcttcttcgg ggtctcggcg gtatggtcag gccagggacg ggtcctgatc
     2641 gaccgggtca ccgtcgtttc ggaggaactc ttccgcgaag aggagctggt gcgccgcggc
     2701 ggcggcatgg agttgcctga cgagtggctg ttgccgccgg ccacgcccgc gcgcatccac
     2761 gtggtcaagg ggctgtggtg ggacttcttc ggtgtgagtg aagcggcggc cgagctgggc
     2821 ggcaccgagc tgacgtcctc ctaccacgcc cag
//
LOCUS       DAOOLN010000103         2843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_43119_length_2843_cov_3.290172, whole genome shotgun sequence.
ACCESSION   DAOOLN010000103 DAOOLN010000000
VERSION     DAOOLN010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43119_length_2843_cov_3.290172"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..1202
                     /locus_tag="PLW80_02010"
     CDS             129..1202
                     /locus_tag="PLW80_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HPB65303.1"
                     /translation="MPSAIRTYGFLSAKLKARISKTMPPETMDRLIKARSLPEAVQFL
                     RGTAYQAIEEAYTATGDLRAGEALLHERELRLYTGLFRYLDEPVLSFVRALAARYEID
                     QVKNAVRLWFDAHVRGRDVAGKSGYLFRGTIVNRMDVDAIVRAADIEGVAAALSGTPY
                     AAIARAELPAAQASRSVFSFELALDRRYYDEAFAAAASLGGRDREAAERALGVDVDLQ
                     NVSWLIRFKDFYGMGAEDAMSRLIPRGRAIKPAAVAAVWDSERGSRGAGGIGLDLLGR
                     SYPGVGAMMGGGGSAAKLAVLEAAIRRVADDEAGKSLCGYPFSIGVVLAYFTKKRAEM
                     RAVMTVLNAKYYALAEERIRSSL"
     gene            1199..>2843
                     /locus_tag="PLW80_02015"
     CDS             1199..>2843
                     /locus_tag="PLW80_02015"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HPB65304.1"
                     /translation="MMFTSPMRLLAAMVLDADSDAIAKELLRLGALEMVSIKELPGDW
                     ASRLGKAEPKVNLARLVELRKRVEGFMDMASPPVARPSLDGSADGEPADLDVAGKRLD
                     AIAADINGLRERQKDLQDQILKLGEIRRQLEAFGDLKAGAAASSAYSFLSIRAGLMPA
                     DVADGLHEALEGIPSVVLPSSGRDAAAASQARRHEAVGVMLVTLKRDAARVEPILERL
                     GWEDARLPESAGQGNAEALETIDRKIDELQARQAACVDDLNAYFTSIGSGLGSMWAAL
                     RTEELIATVRSGFARTDRTVLFSGWIPAEKAAAVEAGIRKAASGACSVEWLEPGKGEA
                     ARLEAPVEMRNPKPLKPFEGLVRNYAVPEYGSVDPTPFVAVAYLCMFGLMFGDAGHGL
                     VVLLVGLLGLASARRSGKPGDLYRVIAYCGGAAIVAGVLFGSYFGMQWLPPLWFDYHG
                     IVNGHEAAGSVRTLYDILGLTIRFGMVVLGLGFAINWVNLFRKRDWFTLLLDKAGVIG
                     GWIYGAGAWAAFYYVRHDYRELPPVGILALLLGLPTMILA"
BASE COUNT          441 a          944 c          999 g          459 t
ORIGIN      
        1 cgcggcccgc gaggcagccg acgccgaggc cgagcggaac gccggtacgg tagcgtcgga
       61 gcgcgagtcg ctcgtcgcgg agatcgtcgg cgtcatcgcc gggcggccgt gatggaggcg
      121 tgagacccgt gccctccgcg atacggacct atgggttcct gagcgccaag ctcaaggcca
      181 ggataagtaa gacgatgcct ccggagacga tggaccgcct gataaaggcg cgatcgctcc
      241 cggaggccgt tcagtttcta aggggtaccg cctaccaggc gatcgaggaa gcctatacgg
      301 cgaccggcga cttgcgagcc ggcgaggccc tgctccacga gcgcgagctc cgcctgtata
      361 ccggcctgtt ccggtacctg gacgagccgg tcctgtcgtt cgtacgggcg ctcgcggcgc
      421 gctacgagat agaccaggtc aagaacgccg tccgcttatg gttcgacgcc cacgtccggg
      481 gacgcgacgt ggccggtaag tccggctacc tgttccgcgg gaccatcgtg aaccggatgg
      541 atgttgacgc tatcgtacgt gccgccgata tcgagggcgt cgccgcggcg ctatctggca
      601 ctccttacgc ggccatagcg cgcgccgagc tacccgcggc ccaggcgtcc cgctccgtgt
      661 tctcgttcga gctggcgctc gaccgccgct actacgacga agccttcgcg gcggcggcca
      721 gcctgggcgg ccgcgaccgg gaagccgccg agcgcgccct cggcgtcgac gtggacctac
      781 agaacgtatc gtggctgata cggttcaagg acttctacgg catgggcgcc gaagacgcca
      841 tgagcaggct gataccccga ggccgcgcta tcaagccggc ggccgtggcg gcggtatggg
      901 atagcgagcg tggttcgcgc ggagcgggcg gcataggctt ggacctactc ggccgctcgt
      961 atccgggcgt cggcgcgatg atgggcggcg gcggttcggc ggccaagttg gccgtgctcg
     1021 aggccgccat acggcgcgtc gccgacgacg aggccgggaa gagcctctgc ggctatccgt
     1081 tctcgatagg cgtcgtgctg gcgtacttca ccaagaagcg cgccgagatg cgcgccgtaa
     1141 tgactgtcct gaacgccaag tactacgcgc tcgccgaaga aaggatacgg agctccctat
     1201 gatgttcacc tctccgatga gactgctcgc ggcgatggtc ctggacgcgg attcggacgc
     1261 catcgccaag gagctcctgc gcctcggggc gctcgagatg gtatcgatca aggaactgcc
     1321 cggcgactgg gcctcgaggc tcggcaaggc cgagcccaag gtcaacctgg ccaggctcgt
     1381 cgagctgcgc aagcgtgtcg agggcttcat ggacatggcc tcgcccccgg tggccaggcc
     1441 gtcgctcgac ggctccgcgg acggggagcc cgccgacctc gacgtcgccg gcaagcgcct
     1501 ggacgctata gcggccgaca tcaacggcct gcgggagcgc cagaaggacc tgcaggacca
     1561 gatcctgaag ctcggcgaga tacgcaggca gctcgaggcc ttcggcgacc tcaaggccgg
     1621 ggcggccgcg tcgtcggcgt attcgttcct gtccatacgc gccggactca tgccggcgga
     1681 cgtcgctgac ggcctccacg aggctctcga gggcataccg tcggtggtgt taccctcgtc
     1741 cgggcgggac gccgcggccg cctcgcaggc tcgtcgacac gaggccgtcg gcgtcatgct
     1801 cgttacgctc aagcgcgacg cggcgcgggt ggagcccatc ctcgagcggc tcggctggga
     1861 ggacgcgagg ctacccgagt cggcgggaca gggcaacgcc gaggcgctcg agacgataga
     1921 ccgcaagata gacgagctcc aggccaggca ggcggcctgc gtagacgacc tgaacgccta
     1981 tttcacgtcg ataggctccg gcctcggctc gatgtgggcc gccctgagga ccgaggagct
     2041 catcgcgacg gtccgctcgg gcttcgcgcg taccgaccgt accgtgctgt tctcgggctg
     2101 gatcccggcc gagaaagccg ccgctgtcga ggcgggtatc cggaaagcgg cctccggcgc
     2161 ctgctcggtc gagtggctgg agcccggcaa gggcgaggcc gccaggctcg aggcgccggt
     2221 ggagatgcgc aacccgaaac ccctgaagcc cttcgagggc ctcgtgcgca actatgcggt
     2281 gcccgagtac ggaagcgtcg acccgacccc cttcgtcgcg gtcgcgtacc tatgcatgtt
     2341 cggcctgatg ttcggcgacg ctgggcacgg cctcgtcgtg ctgctcgtcg gcctcctcgg
     2401 actggcctcg gcgaggcgat ccggcaagcc gggcgacctg taccgagtca tcgcgtactg
     2461 cggcggcgcg gccatcgtcg ccggcgtgtt gttcggctcg tacttcggga tgcagtggct
     2521 gccgccgctc tggttcgact atcacggcat cgtcaacggc cacgaggccg cgggctcggt
     2581 acgcaccctg tacgatatac tgggtctcac catacgcttc ggcatggtcg tgctcgggct
     2641 cggcttcgcg atcaactggg tcaacttgtt caggaagcgg gactggttca cgttactgct
     2701 cgacaaggcg ggcgtcatcg gtggctggat ctacggcgcc ggcgcctggg ccgcgttcta
     2761 ctacgtgcgg cacgactacc gggagctgcc gccggtcggg atcctggccc tgctgctcgg
     2821 ccttcccact atgatcctgg cct
//
LOCUS       DAOOLN010000104         2838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_43228_length_2838_cov_2.921667, whole genome shotgun sequence.
ACCESSION   DAOOLN010000104 DAOOLN010000000
VERSION     DAOOLN010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43228_length_2838_cov_2.921667"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLW80_02020"
     CDS             <1..299
                     /locus_tag="PLW80_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65305.1"
                     /translation="YAFPDGRQGLISAFLTEEPDGVGTLTLADDGVGLPPGVSFDSDQ
                     SLGFQLIHALACQAGATVSARPVEWGARFDIRIAGRAQAGCQDVAALDSRPISG"
     gene            complement(360..1265)
                     /locus_tag="PLW80_02025"
     CDS             complement(360..1265)
                     /locus_tag="PLW80_02025"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB65306.1"
                     /translation="MAKNMKSQPKRCAEPFQGRLVVITGATSGVGFHTARKYASMGAR
                     VLMINRNEDKSERVRKELSDEFGASVDYIIADLAVLADIQRAGRYLADLQAPIDALIH
                     NAGLHLTTRSETIDGLEVNFVLHYLSTFIITSMLLDKYRRDGTGRILFVGSEAYRFAA
                     WGLDLDDLQWERRRYAGIKAYGAGKLAQLLSMHRFAAELAPHGVTVNAMHPGMVRTET
                     GKDNGRLYRWYKKNLIDGRSMSPEVSAEALYYLGASSEPARTSDTFFHLTTEEELAPP
                     ARDLAAAEALWARTVELLRRKGVTA"
     gene            complement(1451..2359)
                     /locus_tag="PLW80_02030"
     CDS             complement(1451..2359)
                     /locus_tag="PLW80_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HPB65307.1"
                     /translation="MTDPLDELAASDFSRARLREALTRVGGFLRPEHQRLLSLDEVRS
                     ILKIRGEEYRGVMPVPLELIIGSEGRYRDFARGFLPRHDYLRDRWIRVDSAHYHDVIL
                     PPVRLYELGGAYFVRDGNHRVSVAKLQGALEIDAEVTSLGSAVRIDKGMDMDALRRAI
                     VGYEKREFYAQTLFGQVTDDPALDFTAPGAYDQIVEHVLVHKYYLNERSPEEISFVDA
                     LDSWYRAVYRPIIEAIEAEGLLSGFRGRTASDLYLYLVKHWDELKRRYGLHYPVSAAA
                     RDFRDRYGRGFLKDAKAFLGSLFKKR"
     gene            complement(2352..2714)
                     /locus_tag="PLW80_02035"
     CDS             complement(2352..2714)
                     /locus_tag="PLW80_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65308.1"
                     /translation="MGLGGSLRYNDGQNQFTQAEMWARVLAKIPALLWNRLVRGRAVD
                     VVLTHAPPRGIHDRPDRCHRGFDAFLWLMRAFRPRFLVHGHVHLYDAKERRVTRYRDT
                     VVVNAYGHAVLDTEASYD"
BASE COUNT          458 a          916 c          950 g          514 t
ORIGIN      
        1 agtacgcgtt cccggatggc cggcaagggc ttatatcggc attcctgacc gaagagccgg
       61 acggcgtcgg cacgctgacg ctcgcggacg acggggtagg cctgccgcct ggggtgagct
      121 tcgacagcga tcagagcctg ggtttccagt tgattcacgc cctggcctgt caggcggggg
      181 cgacggtgtc ggcgcgcccc gtagagtggg gcgcgcgctt cgatatccgt atcgccgggc
      241 gcgcccaggc cggctgtcag gacgttgccg cgcttgattc ccgcccgatc agcggatgac
      301 cgacgtcgct acggcccgtc ccgccatgac gcctcccatg gcgacgtaag cctcgtacat
      361 tacgccgtca ctcctttgcg gcgaagcagc tcgacggtcc gcgcccacaa cgcctccgcc
      421 gcggcgagat cccgggccgg aggcgccagt tcctcctccg tcgtcaagtg gaagaacgta
      481 tcgctcgtcc gggccggttc gctcgaggcg ccgagatagt acagcgcttc agccgaaacc
      541 tcgggcgaca tcgatctgcc gtcgatgagg tttttcttgt accatcggta gagcctgccg
      601 ttgtccttgc ccgtctccgt acggaccatg cccgggtgca tggcgttcac ggtcacgccg
      661 tggggcgcga gctcggcggc gaagcggtgc atcgacaata gctgcgccag cttaccggct
      721 ccgtacgcct tgatcccggc gtagcgccgt cgttcccact gcaggtcgtc caggtcgagg
      781 ccccaggccg cgaaacggta cgcctcggag ccgacgaaga gtatccgtcc cgtcccatcc
      841 ctccggtatt tatccagaag catcgacgtg atgatgaacg tcgagaggta atgcagtacg
      901 aagttgacct ccaagccgtc gatcgtttcc gaccgcgtgg tcaggtgcag gccggcgttg
      961 tggatgagcg cgtctatcgg cgcttgaagg tccgctaggt agcgtccggc ccgctggatg
     1021 tccgcgagga cggcgaggtc cgcgatgatg tagtccacgg acgcgccgaa ctcgtcggac
     1081 agctccttgc gcacgcgctc ggacttatcc tcattcctgt tgatcatgag cactcgagcg
     1141 cccatggagg cgtacttacg cgccgtatgg aaaccgacgc ccgaggtggc gccagtgatg
     1201 accaccagcc gtccttggaa cggttcagcg cagcgtttgg gctggctctt catgttcttc
     1261 gccatcgcga gcacgttcga ccacgtatac tgaggccaat atcgccgtat cgctgggttc
     1321 atgtcgctgc gctcctcgcg ttacgagatt ctactgtatc cgctacgtct gatacaggta
     1381 tgaggattgg tttggatcct gtagcgattc cgcgcccgtt tgcactcgcc tgaagcgcca
     1441 ttcagaagga tcagcgcttc ttgaatagcg agccaaggaa ggccttggcg tccttcagga
     1501 atccgcggcc gtagcggtcc ctgaaatcgc gggcggccgc ggagacgggg tagtgcaggc
     1561 cgtagcgccg tttgagctcg tcccaatgct tgacgaggta caggtagagg tcgctggcgg
     1621 tgcgtccgcg gaagcccgac agcagccctt ccgcctcgat ggcttcgatg atgggcctgt
     1681 agaccgctcg gtaccaggag tcgagcgcgt cgacgaagct gatctcctcg ggcgatcgct
     1741 cgttcaggta gtacttatgg accagcacgt gctcgacgat ctggtcgtag gcgcccggcg
     1801 cggtgaagtc gagggctggg tcatccgtca cctggccgaa cagggtctgc gcgtagaact
     1861 cccgcttctc gtagccgacg atggcccgcc tcagggcgtc catgtccatg cccttgtcga
     1921 tgcgcacggc cgagccgagc gaggtgacct cggcgtctat ctcgagcgcg ccctgcagct
     1981 tggccaccga tacgcggtgg ttgccgtcgc gcacgaagta cgccccgccg agctcgtaga
     2041 gcctgaccgg aggcaggatc acgtcgtggt agtgggccga gtcgacgcgt atccagcggt
     2101 cgcgcaggta gtcgtgccgg ggcaggaagc cgcgcgcgaa gtcgcggtag cggccctccg
     2161 agcctatgat cagctcgagc ggcaccggca tcacgccgcg gtactcctcg ccgcgtatct
     2221 tgagtatcga acgcacctcg tcgagcgaca gcaggcgctg gtgttccggc cggaggaagc
     2281 cgccgacgcg cgtcagcgcc tcgcgtagcc gggccctgga gaagtcgctg gccgccagct
     2341 cgtcgagcgg gtcagtcata gctcgcctcc gtgtccagga ccgcgtggcc gtacgcgttg
     2401 accacgaccg tgtcgcggta gcgcgttacc ctccgctcct tggcgtcgta gagatgcacg
     2461 tggccgtgca cgaggaagcg cggcctgaag gcccgcatca gccagaggaa cgcgtcgaag
     2521 ccgcggtggc agcggtctgg cctgtcatgg atgccgcgcg gcggcgcgtg cgtcagcacc
     2581 acatccacgg cccggccgcg gaccaggcgg ttccacagca acgccgggat cttggccagc
     2641 acgcgggccc acatctcggc ctgggtgaac tggttctggc cgtcgttgta gcgtagcgag
     2701 cctccgaggc ccatgacgat caggtcagct tcgcgcagca gcttgaagcc gacgtaggtg
     2761 gcgccgacgt ccctggatag gtcgtggggc gacggcggcg gcgtctacgt cgaaggaaac
     2821 ggcgccagca gcgcgacg
//
LOCUS       DAOOLN010000105         2837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_43252_length_2837_cov_3.054277, whole genome shotgun sequence.
ACCESSION   DAOOLN010000105 DAOOLN010000000
VERSION     DAOOLN010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43252_length_2837_cov_3.054277"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PLW80_02040"
     CDS             <1..376
                     /locus_tag="PLW80_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB65309.1"
                     /translation="RAAIARALIVRPELLLADEPTGNVDPTMARRIIRLFVELQRSGT
                     SVVVATHDLGLMDQFDGARRLVLGDGRLGAGTVSSRGDHRIAMAAAVAALAGSGPVAI
                     DAAECVSKSWPGFYGALDSIRA"
     gene            complement(366..1223)
                     /locus_tag="PLW80_02045"
     CDS             complement(366..1223)
                     /locus_tag="PLW80_02045"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HPB65310.1"
                     /translation="MRVAILGAGKMGAWLIRSLAAAGHELAAYDPSPGRLGAATAGVV
                     GSATGKPVEAADDVRALAAFGPELLVNCAPLGLTTEAFDAVTPHLDEACALSDIASVK
                     TGLASWYASSGRPFSSSHPMFGPTYADGSNLSEQSAVIIAESSDDGKAFWKSFYGSFG
                     IRVFEESFDGHDRTVAYSLGTPFASTMVFAACMKRLDAPGTNFRKHLDIARGLLAEDD
                     RLLAEIMFNPYTIRRLELINSQLAYLTHIIKDRDHEEMGKFLDKLRGNLGEAPDGPAG
                     YAAAPPTRP"
     gene            complement(1225..2382)
                     /locus_tag="PLW80_02050"
     CDS             complement(1225..2382)
                     /locus_tag="PLW80_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HPB65311.1"
                     /translation="MTLESLRRDIDRIDARILSLLDERLEKGLLTRRHKSDSLDSGRE
                     AEVLGRVSARSRCLADEAFTTGLYRRIMDESKAIQDRRLSLIGFQGMHGAYGEAAARA
                     WAPEAATMPFGEFSQVFDAVLAGDVDYGIVPVENTLGGVVGQVNSILVTTELRVAGAV
                     DMAVSHCLLAPPGADHRELRTVYSHGQALSQCRRFIERNGLEGVDWFDTAGAARMIAE
                     ERPKGAAAIASRFAAELYGLEIIKDGIQDSPNNRTRFFVLAKEARQATNGPADGDHGR
                     AGDDGRAGDDGRAGDHGKAGDDGRAGDKCSAVFFADDKAGALFAVLEVFAEAGINLTR
                     IESVPTEPGDYAIFIDFAGSDRDEAVIKALDRAGGLSRGFRLLGCYAERRL"
     gene            complement(2379..>2837)
                     /locus_tag="PLW80_02055"
     CDS             complement(2379..>2837)
                     /locus_tag="PLW80_02055"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPB65312.1"
                     /translation="GLRAGLGEPFFGALESLAAAALFAVPGVRGVEFGDGFAAARMRG
                     SEHNDPIVAPDGAMSKNGSGGVNGGISNGNELVVRVAMKPASTIAKPQRTWNFATGAM
                     DELSAPGRHDACIALRGAVALEAALAVVLADLELQALARGASLPNGGIPS"
BASE COUNT          401 a          959 c         1025 g          452 t
ORIGIN      
        1 acgcgccgcc atcgcccggg cgctgatcgt aagacccgaa cttctcctgg cggatgaacc
       61 gaccggcaac gtcgacccga caatggcgcg tcgaatcatc agattgttcg tcgagctcca
      121 aaggtcgggc acatcggtgg tcgtggcgac tcacgatctc ggcctcatgg accagttcga
      181 cggcgcccgc cgtctcgtcc tgggcgacgg ccgcctgggc gccgggacgg tatcgtcgcg
      241 aggcgaccac cgtatagcga tggccgccgc cgtggccgcc ctcgccggct cgggcccggt
      301 ggccatcgac gccgccgagt gcgtgtccaa gagctggccg ggcttctacg gggcgctcga
      361 ctcgatcagg gcctagtcgg cggcgcggcg gcgtacccgg ccgggccgtc gggagcctcg
      421 ccgaggttgc ctcgcagctt gtccaggaac ttgcccatct cctcgtggtc gcggtccttg
      481 atgatatggg tcaggtaggc cagctgggag ttgatcagct ccaggcgccg tatcgtgtac
      541 gggttgaaca tgatctcggc gagcaggcgg tcgtcctcag cgagtaggcc gcgcgcgatg
      601 tccaggtgct tgcggaagtt ggtgccgggc gcgtcgaggc gcttcatgca ggcggcgaag
      661 accatcgtcg aggcgaaggg cgtgcccagc gagtaggcga ccgtccggtc gtgcccgtcg
      721 aagctctcct cgaagacccg gatgccgaag gatccgtaga acgacttcca gaaggccttg
      781 ccgtcgtcgc tcgactcggc gatgatcacc gcgctctgct cgctcaggtt cgagccgtcg
      841 gcgtaggtcg ggccgaacat cgggtgggac gacgagaagg gccggcccga cgaggcgtac
      901 catgacgcga ggccggtctt gaccgaggct atgtcggaca gcgcgcaggc ctcgtccaga
      961 tggggcgtca cggcgtcgaa ggcctccgtc gtcaggccga gcggcgcgca gttgacgagc
     1021 agctccggcc cgaaggccgc cagggcgcgg acgtcgtcgg cggcctcgac gggcttcccg
     1081 gtggccgagc cgacgacgcc ggcggtcgcc gcccccaggc gaccgggtga cgggtcgtag
     1141 gccgccagct cgtggccggc ggccgcgagg gaacgtatca gccaggcgcc catcttgccg
     1201 gcgccgagta tcgcgacgcg cacgctacag cctcctctcg gcgtagcagc cgagcaacct
     1261 gaagcctcgc gacaggccgc cggcgcggtc gagcgccttg atcaccgctt cgtcgcggtc
     1321 ggagccagcg aagtcgatga agatggcgta gtcgccgggc tcggtcggca ccgactcgat
     1381 gcgcgtcagg ttgatgcccg cttcggcgaa cacctcgagc acggcgaaca gggcgccggc
     1441 cttgtcgtcg gcgaagaaga cggccgagca cttgtcgccg gctctgccat cgtcgccggc
     1501 tttgccgtgg tcgccggctc tgccatcgtc gccggctctg ccgtcgtcgc cggctctgcc
     1561 gtggtcgccg tcggccggtc cgttcgtggc ttggcgagcc tccttggcga gcacgaagaa
     1621 gcgcgtgcga ttgttcggcg agtcctggat gccgtccttg atgatctcga gcccgtagag
     1681 ttcggcggcg aagcggctgg ctatggccgc cgcgcctttc ggacgttcct cggctatcat
     1741 gcgggcggcg cctgcggtgt cgaaccagtc gacgccctcg aggccgttgc gctcgatgaa
     1801 gcgccggcac tgcgacaggg cctggccgtg ggagtagacc gtgcgcagct cgcggtggtc
     1861 ggccccgggc ggcgccagca ggcagtggct gacggccatg tcgacggcgc cggcgacgcg
     1921 cagctcggtc gtcaccagta tcgagttgac ctggccgacg acgccgccga gggtgttctc
     1981 gaccggtacg atgccgtagt cgacgtcgcc ggccagcacg gcgtcgaaga cctgggagaa
     2041 ctccccgaaa ggcatggtcg ccgcctcggg ggcccaggct ctcgccgccg cctcgccgta
     2101 ggcgccgtgc ataccctgga agcctatcag cgagagcctt cggtcctgga tggccttgct
     2161 ctcgtccatg atgcgtcgat acagccccgt ggtgaaggcc tcgtccgcca ggcagcgcga
     2221 gcgggcggac acgcgcccga gcacctccgc ctcgcggccg gagtcgagcg agtcgctctt
     2281 gtggcggcgc gtcaagaggc ctttctcgag cctctcgtcg agcagggaca ggatgcgggc
     2341 gtcgatgcgg tctatgtccc gcctgagcga ttcgagggtc atgatggtat tcctccgttc
     2401 ggtagcgagg ctcctcgggc gagcgcctgg agctcgaggt cggccaggac gacggcgagg
     2461 gccgcctcga gggccacggc gccgcggagc gcgatacagg cgtcgtgcct gcccggcgcc
     2521 gacaactcgt ccatggctcc cgtagcgaag ttccaggtgc gctgaggctt ggctatggtc
     2581 gaggccggct tcatcgcgac gcggacgacc agctcgttgc cgttcgatat gccgccgttg
     2641 acgccgcctg agccgttctt ggacatggcc ccgtccgggg cgacgatggg gtcgttatgc
     2701 tcggagcctc gcatgcgggc cgcggcgaag ccgtcgccga attctacgcc gcgcacgccg
     2761 ggcaccgcga acagggccgc cgcggccagc gactccagcg cgccgaagaa tggttcgccg
     2821 aggccggccc gtagccc
//
LOCUS       DAOOLN010000106         2836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_43264_length_2836_cov_3.600863, whole genome shotgun sequence.
ACCESSION   DAOOLN010000106 DAOOLN010000000
VERSION     DAOOLN010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43264_length_2836_cov_3.600863"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLW80_02060"
     CDS             <1..192
                     /locus_tag="PLW80_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65313.1"
                     /translation="RVEYGAPGLGIAELRDDSGLPRLAHEWIAFERMMREGRLDECYD
                     LLEHSLRVMKVLDSLSRGL"
     gene            483..1595
                     /locus_tag="PLW80_02065"
     CDS             483..1595
                     /locus_tag="PLW80_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB65314.1"
                     /translation="MKEKARTTILIVENDVLSALNVRETLEAAGYATLSTGTGEDAVR
                     LASDSSVGLVLMDISLDGELDGIDAAEAILRASSRPIVFMSSHDDAATMIRLERVDAY
                     GFMPKPFGNASLLATVSMALRRSAAERSAADRMVADYERRLLQSDTRLKESHHRIRND
                     IAAISSILSLQLGEAEHPETVEALSDALGRVESLSTLYEMLTVHDSGGDIEVRPYLER
                     LIGAIMPIFPGGSGIDVDCRVDEFMLDQKRTFTLGIIVNELLTNALKYAFDGHSEGSI
                     RLDVCVYDESVIMTMRDDGKGLPERFDIAESSGLGLTLISMLLDQFDGTLTMRSDHGT
                     VSTALIKAPGLEATAPAAPAGLEAAIVAKRARVNVA"
     gene            complement(1618..>2836)
                     /locus_tag="PLW80_02070"
     CDS             complement(1618..>2836)
                     /locus_tag="PLW80_02070"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPB65315.1"
                     /translation="DRLNGKPYLPLHPLIGDLLAESYGIMCYQEDVCKVAVALAGFSP
                     AEADKVRKALSKKDAPERLAAYAGRFRDGALARGVDERTIAETWAMIESFSGYSFVKA
                     HSASYAMLSFRSAYLRRHHPAEFMAAVISNRGGYYSTLAYVSEARRMGLRVLPPDVET
                     SRLRCRGRGSELRLGLGMVSTLSSACSEAIVRARRAGGPFRSVDALARRARVGRPDAE
                     ALVGSGALDSLSPGMTRADKLARLLSLCDEGKREARQGELFASEPAVPGPARPPGSSG
                     PSGPGARKRLESELLYLGTTLAEHPLALWPKALSMPRALGRDLPRLVGRRVALIGWPI
                     TAKPVVTSGGEAMEFVSFEDETALYETVLFPGAFARYRHLLFDERPLIVRGVVEEDRT
                     AVTVTVDSLERLG"
BASE COUNT          511 a          951 c          911 g          463 t
ORIGIN      
        1 cgcgtcgagt acggagcgcc gggcctaggc atcgccgagc tacgcgacga ctccggccta
       61 cctcggctag cccacgagtg gatagccttc gagcggatga tgcgtgaagg ccgactcgat
      121 gagtgctacg acctactcga acacagcctg cgggtcatga aggtactgga tagcctgagc
      181 cgcggactgt gacgcgttga gccggtatag acatagagtt attcctatat gacaccgcat
      241 aatatttgat atttctaacg aatttgaggt gattttgacc cttgtagaca attgtctaca
      301 cgcttcgggc aaaaaacagc ttgatcggct ctacacccgg agatacgatc cacgtaatga
      361 aaacgctttc aaaatcattt cataagaatt tatataaacg tagtgcgtac gaacgcgatc
      421 ggcgtagcct agctaggccg atcgcgttgt atgcgtcgaa tccgtccgat ggagcaagcg
      481 gagtgaagga gaaggcgcgc acgaccatcc tcatcgtcga gaacgacgtc ctgagcgcgc
      541 tcaacgtacg ggagacgctc gaggccgctg gatacgccac tctatccacc ggtacgggcg
      601 aagacgccgt gcgcctcgcc tcggactcgt ccgtcggcct cgtcctgatg gatattagcc
      661 tcgatgggga gctggacggc atcgacgcgg ccgaggcgat cctgcgcgcc tcctcgcgac
      721 caatcgtgtt catgtcttcc cacgacgacg cagcgacgat gatccgcctc gagcgcgtcg
      781 acgcgtacgg cttcatgccc aagccattcg gcaacgcgtc gctcctggct accgtatcga
      841 tggcgctcag gcgatccgcg gccgagcgtt cggcggcgga tcgtatggtc gccgattacg
      901 agcggcgcct cctgcagagc gacacgcggc tcaaggagtc tcatcaccgt atccgtaacg
      961 atatagcggc gatctcgtcc atcctgtctt tgcagctcgg cgaggccgag catcccgaga
     1021 ccgtcgaggc cctcagcgac gcgctcggcc gcgttgaaag cttgagcacg ctctacgaga
     1081 tgctcaccgt acacgactcg ggcggcgata tcgaggtgcg cccgtacctg gagcgcctca
     1141 taggcgccat catgccgatc ttccccggcg gctccgggat cgacgtcgat tgccgcgtcg
     1201 acgagttcat gctcgaccag aaacggacct tcacgctggg catcatcgtc aatgaactcc
     1261 tgacgaacgc gctcaagtat gccttcgatg gccacagtga aggttccata cgcctcgacg
     1321 tctgcgtgta cgacgagtcg gtcatcatga cgatgcggga cgacggcaag ggccttcccg
     1381 agcgcttcga tatagccgag tcgagcggcc tgggtctaac gctgatatcg atgttgctcg
     1441 accagttcga tggtacgctc accatgcgca gcgaccacgg gactgtcagc accgcgctga
     1501 tcaaggcgcc cgggctcgag gccaccgcgc cggccgctcc ggctggcctc gaggcggcca
     1561 tcgtcgcgaa gcgcgcccga gtcaacgtcg cctgagccgt tccggcgccg ccctccccta
     1621 tcctagccgc tccaggctat ccacggtcac ggtcacggcc gtccggtcct cctcgacgac
     1681 gccccggacg atgagcggcc gttcgtcgaa aagcaggtga cgataccgcg cgaacgctcc
     1741 ggggaacagc accgtctcgt acagcgcggt ctcgtcctcg aacgacacga actccatagc
     1801 ctcgccgccc gaggtgacga cgggcttggc cgttatcggc cagcctatca gcgcgacgcg
     1861 gcggccgacc aggcgcggca ggtcgcgccc gagcgcgcgc ggcatggaga gcgccttggg
     1921 ccatagggcc agcggatgct ccgccagcgt cgtgccgagg tagagcagct ccgactcgag
     1981 ccgcttgcgc gcgcccggcc ccgacggccc cgacgatccc ggcggccgcg ccggaccggg
     2041 gacggcgggt tcggaggcga acagctcgcc ctggcgggcc tcccgcttgc cttcgtcgca
     2101 tagcgacagg agccgcgcga gcttgtccgc ccgcgtcatg ccgggcgaca gcgagtcgag
     2161 ggcgcccgag ccgactagcg cctccgcgtc gggtctcccg acgcgggccc tgcgcgccaa
     2221 ggcgtcgacc gagcggaacg gcccaccggc gcgtcgcgcg cggactatgg cctcggagca
     2281 cgcggacgac agcgtcgaga ccatcccgag gccgagccgt agctccgagc cccggcctcg
     2341 acagcggagg cggctcgtct cgacgtcggg cggcagcacg cggaggccca tgcgcctggc
     2401 ctcgctgacg taggccagcg tcgagtagta gccgccgcgg ttcgagatga ccgcggccat
     2461 gaactccgcg ggatggtgac ggcgcaggta ggccgaacgg aacgacagca tcgcgtagct
     2521 ggccgagtgc gccttgacga acgagtagcc ggagaacgac tcgatcatcg cccaggtctc
     2581 ggctatcgtc cgctcgtcga ccccgcgggc cagcgcgccg tcgcggaagc ggccggcgta
     2641 ggctgccagg cgctccggag cgtccttctt ggacagcgcc ttgcgcacct tgtcggcctc
     2701 ggccggtgaa aagccggcca gggccaccgc gaccttgcag acgtcctcct ggtagcacat
     2761 gatgccgtaa ctctcggcga gcaggtcgcc gatcagcggg tgcagcggca gatagggctt
     2821 gccattcagc cggtcg
//
LOCUS       DAOOLN010000107         2832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_43357_length_2832_cov_2.818149, whole genome shotgun sequence.
ACCESSION   DAOOLN010000107 DAOOLN010000000
VERSION     DAOOLN010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43357_length_2832_cov_2.818149"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1290..2195
                     /locus_tag="PLW80_02075"
     CDS             1290..2195
                     /locus_tag="PLW80_02075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB65316.1"
                     /translation="MVGAASEFEEALSEQLKNDTLCQRPVVEVGREIVAYGKIEQWKE
                     GDGTNVYLLMGWVHPRARGRGIGTALLSWLEGEAAALAQRDHPGEPSEFAGNASSTET
                     SATKLLTDAGYRAAYTVAELRLDSSSHAEARAPALPDGLVIVAPGKDDYRKLTGTVVT
                     AFAKEYSEGRFDEPVDPGEYEESLSDDDREPSLWRIVKRGDEIVAVALCDARTDAMAE
                     VVELAVSPDWRRRGIARALMTATIDAAIAAGFRELRVYTIEQFQTRAIQLYQSLGFAR
                     YKDFPRYRKPMPGLPPRTLDARIGA"
     gene            2192..2641
                     /locus_tag="PLW80_02080"
     CDS             2192..2641
                     /locus_tag="PLW80_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HPB65317.1"
                     /translation="MIPDKVLSALDAHGLSALEFEPGSTPTVELAAARIGVEPARIAK
                     SLLFKSKDERYVMLVCPGDKRVQSGALKRLAGSKLSMTDAEETERVTGYKPGGVCPFA
                     LTGVEVLLDRELGDYEIVYPAAGTDATGVPVSLAQLEAATGGRIVDL"
BASE COUNT          588 a          858 c          865 g          521 t
ORIGIN      
        1 tgctagtact cgctccatca tgttcactcg cccttaagct ataaatatat ggctataaac
       61 ccgtacgatt cctattaggc accttgctca atacccattg gacaccttag actgtctacc
      121 atgctgcaat ccagttcacg cacgctacta tcccaaaacc ttaggcgata ccgcgcgatc
      181 catcatcttt ctcaagccga tttagccgaa gccgtcgggt gttcggcgac acttataacc
      241 aacatcgaac tcatgaagcg cttcccgtca ccggagaatc tcgatcggat cagcgcagcg
      301 accggcattc cggtatccga gctgttccgg gatgaatcgc cggatatggc gtgtcttcaa
      361 gagctctcgt tgatgcgcgc taggcttgag tccggcttca aacggttact cgacgaggct
      421 ctgcaagtag ctccgcgaga ctgactattg gggctcctgt tactaaatct gttaccggaa
      481 ccttgcccat tttctaagta aagtcctata atgccttagc aaggtttata aaggccgttg
      541 gcctatttcc ttgccggaca atagtaacag tgacacttta taaagctatt tcattacctg
      601 gtaagcactt gatgactttc gggaccaaga tgtcggaggt tcaaatcctc tcagcccgac
      661 ttaaaaataa tgggtcaccc tccttgggtg gcccgttgtt tttatatccg agcgggctga
      721 gaggatttga accggagcga gcgccgagcg atagcgagga tgagcgccca tggatgggcg
      781 cggaagcgag cggaggcggt cgcaaggagc gcgcacgatg cgcgcgactg gagaccaaat
      841 cctctcagcc cgacttaaaa ataatgggtc accctccttg ggtggcccgt tgtttttata
      901 tccgagcggg ctgagaggat ttgaaccgga gcgagcgccg agcgatagcg aggatgagcg
      961 cccatggatg ggcgcggaag cgagcggagg cggtcgcaag gagcgcgcac gatgagcgcg
     1021 actggagacc aaatcctctc agctcgaggt attcgagaac gatccggcat cgcttgcgct
     1081 gatcgtcggc ttcctcgata ctgttcccat ggaaaacata acacccttac cagaactagc
     1141 gggagccgtg ttcaggttcg cgcgcgggcc tgaagacggg gcggcgttgg ccgcgattca
     1201 cgacgcacgc ctcggaatag gcggcgttgg ccgcgattca cgacgcacgc ctcggaatag
     1261 acggcgtcga cccaagcgac tcctatgaga tggtcggcgc cgcgtcagag ttcgaagaag
     1321 ccctctcgga gcaactgaaa aacgacacgc tctgccagcg acccgtcgtc gaagtgggca
     1381 gagagatcgt cgcctacgga aaaattgaac aatggaagga aggcgatgga accaacgtct
     1441 atctgctgat ggggtgggtt catcccaggg ccagggggcg aggcatcggc accgccctcc
     1501 tctcatggct ggagggcgag gcggcggcgc tcgctcagcg cgatcacccc ggagaaccga
     1561 gcgaattcgc aggcaacgcc tcttcgaccg agacgagcgc gacgaagctc ctcaccgatg
     1621 ccggctatcg cgcggcgtat accgtggcgg agctacggct cgactctagt agccacgccg
     1681 aggctcgggc tccggccctg ccggacggcc tcgtcatcgt cgcccccggg aaggacgatt
     1741 atcggaagct gaccggaacg gtcgtcaccg ccttcgccaa ggagtactcg gaaggcaggt
     1801 tcgacgagcc ggtcgatccg ggcgaatacg aggaaagcct ttcggacgac gatcgagagc
     1861 cgtcgctctg gcgaatcgtc aagcgaggcg acgagatcgt ggcggtcgcg ctctgcgacg
     1921 ctcgaaccga cgccatggcc gaggtcgtcg agctcgccgt ttccccggac tggaggcgca
     1981 ggggcatagc ccgcgccttg atgacggcga ccatcgatgc cgcgatcgcg gccggattca
     2041 gggagctacg cgtatacacg atcgagcagt tccagaccag ggcgatccag ctctaccaat
     2101 cgctcggctt cgcccgttac aaggacttcc cccgataccg caagccgatg ccaggcttgc
     2161 ctccacgaac cctggacgct agaatcggtg catgattccc gacaaggtac tcagcgcgct
     2221 cgacgctcac gggctgagcg ctctcgaatt cgaacccggc tccaccccga cggtggagct
     2281 cgccgcggcg cgcatcggcg tcgagcccgc ccggatcgcc aagtccctac tgttcaagtc
     2341 caaggacgag cgctacgtca tgctcgtctg cccgggcgat aagcgggtac aatccggggc
     2401 cctcaagcgc ctcgctggct ccaagctgtc gatgaccgac gccgaggaga ccgagcgcgt
     2461 caccggctat aagccaggcg gcgtatgccc tttcgccctg accggagtcg aggtactgct
     2521 cgatcgcgag ctcggcgact acgagatcgt ctatccggcg gccgggaccg acgctaccgg
     2581 cgttccggtc agtctggcgc aactcgaggc cgccacgggc ggcaggatcg tcgatctctg
     2641 agacgggggt atcgactatg aggttaggca tcgccggtag cggcatgatc gtcgaggatg
     2701 gcctgcgagc catctccgag gcgaagggcg taactgccgt cgccatctgc gcgcgcgaga
     2761 agagccgcga tagggccgag gccctggccg cccgtcatgg cgtcggctcg acggtgtacg
     2821 actacgacga gc
//
LOCUS       DAOOLN010000108         2830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_43401_length_2830_cov_3.624505, whole genome shotgun sequence.
ACCESSION   DAOOLN010000108 DAOOLN010000000
VERSION     DAOOLN010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43401_length_2830_cov_3.624505"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /gene="der"
                     /locus_tag="PLW80_02085"
     CDS             complement(<1..852)
                     /gene="der"
                     /locus_tag="PLW80_02085"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPB65318.1"
                     /translation="MNEETTIRPSRTRWSGLPVVAIAGRPNVGKSTLFNRLLRSRRAI
                     VDPTPGVTRDPIEGEWLPDGADMPVTLVDTGGLKLDRDDMDDRVAKKSWERVASADLV
                     LFLLDAVDITPEDEEFASMLRRYAGKTVLVVNKADSPERDAAAWAHASWGYKDMVFIS
                     AEHGRNIDELAALIVSRLDWSGAKAEDEEHDDVRLAIMGKPNVGKSTLLNKLLGEERS
                     IVSDMPGTTRDVVEGRFEWNGRAFTVLDTAGMRRKAKVTEAVEYYSVNRAVKTLDRAD
                     VVVLMIDA"
     gene            complement(914..2656)
                     /gene="ptsP"
                     /locus_tag="PLW80_02090"
     CDS             complement(914..2656)
                     /gene="ptsP"
                     /locus_tag="PLW80_02090"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HPB65319.1"
                     /translation="MRTLQGLPASPGIAIGSAFMFLDDEEPTIPRYAVTESELDAEWD
                     RFVVAVGKAKDDVEALRDRAVREMGQEHGAIFDSHLIMLDDPDLLESIEASLRSSLLN
                     MEWIVHQYAQAIVKRLEGLDDPILQERTVDVHDITRRILNHLMFRERFSLADLNSDVV
                     LVARNLLPSDMIAMNRSKVRAIVMDAGGKTSHTAILARAFEIPAVLGLGGSARAIRNG
                     DIVVVDGNTGDVRVQPDPQTLERYERIRARMAERDETLSGLASLPAKTLDGKEVLIKA
                     NIEVPEETDSVLRHGAQGIGLFRSEFLFLQPGRAPSEESQYVAYGRVIEAMGDRPVTI
                     RTLDVGGDKVVPDLAIEEEKNPLLGWRAIRYCLSDKELFQSQLRAILRASVSGDARIM
                     FPMISGPVELDSALAQLEAAKAECRRRGQAFREDIPVGIMIEVPSAAMIADILAPKVD
                     FFSIGTNDLIQYTIAVDRGNERVAYLHEPFHPAVVRLVKKVIDDGHAAGIPVSMCGEM
                     ASDPFAAVVLLGLGLDEFSMSAVSVPDVKRTIRSIGFAEARAVADEVLKLGSHRETEE
                     YLAKRLADRVSRED"
     gene            complement(2708..>2830)
                     /locus_tag="PLW80_02095"
     CDS             complement(2708..>2830)
                     /locus_tag="PLW80_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPB65320.1"
                     /translation="YVTTTTVIGAVGSTGYSTGPHLHFTVSKNGRTLNPMSVLR"
BASE COUNT          433 a          926 c          956 g          515 t
ORIGIN      
        1 ggcgtctatc atcagcacga cgacgtcggc ccggtccagg gtcttcaccg cgcggttgac
       61 cgagtagtac tcgacggcct ccgtcacctt ggccttgcgc cgcatgccgg cggtgtcgag
      121 cacggtgaag gccctgccgt tccactcgaa acggccctcg accacatcgc gggtcgtgcc
      181 gggcatgtcg ctgacgatgg aacgctcctc gccgagcaac ttgttgagca acgtggactt
      241 gccgacgttc ggcttgccca tgatggcgag gcgcacgtcg tcgtgctcct cgtcctcggc
      301 cttggcgccg gaccagtcga gcctcgagac gatcagagca gcgagctcgt cgatattgcg
      361 accgtgctcc gcggagatga agaccatgtc cttgtagccc cacgacgcgt gggcccaggc
      421 ggccgcgtcg cgctcggggc tgtccgcctt gttgacgacg agcaccgtct tgccggcgta
      481 gcgcctgagc atcgacgcga actcctcgtc ctcgggagtg atatcgacgg cgtcgagcag
      541 gaacaagacg aggtcggccg aggcgacgcg ctcccagctc ttcttggcca cgcggtcgtc
      601 catatcgtct cggtcgagct tgaggccgcc cgtatcgacg agggtaaccg gcatatcggc
      661 gccgtcgggc agccactcgc cctcgatggg gtcgcgcgtg acgcccggcg tcgggtcgac
      721 gatggcccgc ctcgagcgga ggagtcggtt gaacagcgtc gacttgccga cgttcgggcg
      781 cccggctatg gctaccacgg gaaggccgct ccaacgcgtg cgggagggcc tgatggtcgt
      841 ttcttcgttc atgtgcggta aaggatacac cggaacgggc ggattggact agggttcggc
      901 cggggctccc gccctagtcc tcccgcgaca cccggtcggc cagccgcttc gcgaggtact
      961 cctccgtctc ccggtgggaa cccagcttga gcacctcgtc cgcgacggcc cgcgcctcgg
     1021 cgaagccgat cgagcgtatg gtccgcttga cgtcggggac cgagacggcc gacatcgaga
     1081 actcgtcgag gccgaggccg agcagcacga cggccgcgaa ggggtcgctg gccatctcgc
     1141 cgcacatcga caccgggatg ccggcggcgt ggccgtcgtc gatcaccttc ttgaccagac
     1201 ggaccacggc gggatggaac ggctcgtgca ggtaggctac ccgctcgttg cctcggtcga
     1261 ccgctatcgt gtactggatc aggtcgttcg tgccgatcga gaagaaatcc accttcgggg
     1321 ccaggatatc ggctatcatc gccgcgctcg gcacctcgat catgatgccg accggtatgt
     1381 cctcacggaa ggcctggccc cgtcgcctgc actcggcctt ggccgcctcg agctgggcca
     1441 gggcggagtc gagctcgacc gggcccgaga tcatcgggaa catgatgcgc gcgtcgcccg
     1501 atacgctggc gcgcaggatg gcccggagct ggctctggaa caattcctta tccgaaaggc
     1561 agtagcggat ggcccgccac ccgagcaggg ggttcttttc ctcctcgatg gccaggtcgg
     1621 ggaccacctt gtccccgccg acgtcgaggg tgcgtatggt gaccggccgg tctcccatcg
     1681 cctctatcac ccggccgtag gcaacgtact gcgattcctc gctcggggcc cggccgggct
     1741 gcaggaacag gaattccgac cggaacaggc cgatgccctg ggctccgtgg cgtaacacgg
     1801 aatcggtctc ctccggcacc tctatgttcg ccttgatcag cacttccttg ccgtcgagcg
     1861 tcttggccgg tagcgaggcc aggccgctca gcgtctcgtc gcgctcggcc atcctggctc
     1921 ggatgcgctc gtagcgctcg agggtctgcg ggtccggctg cacccggacg tcgccggtgt
     1981 tgccgtccac gacgacgatg tcgccgttcc tgatggctcg agccgagccg ccgaggccga
     2041 gcaccgccgg tatctcgaag gccctggcga ggatggccgt gtggctggtc ttgccgccgg
     2101 cgtccatcac gatggccctg accttggagc ggttcatcgc tatcatgtcc gagggtagca
     2161 ggtttcgggc gaccaggacg acgtcggagt tcaggtcggc gagcgagaag cgctcgcgga
     2221 acatcaggtg gttgagtatg cgcctcgtta tgtcatgcac gtctaccgtg cgctcctgca
     2281 gaatggggtc gtccaggccc tctaggcgct tgactatggc ctgggcgtac tggtggacta
     2341 tccactccat gttgaggagg ctcgaccgca gggaggcctc gatggactcc agcaggtccg
     2401 ggtcgtcgag catgatcagg tgcgagtcga agatggcgcc gtgctcctga cccatctcgc
     2461 ggaccgcccg gtcgcgtagc gcctcgacgt cgtccttggc cttgccgacg gccacgacga
     2521 accgatccca ctccgcgtcc agctcggact cggtgacggc gtacctgggg atggtcggct
     2581 cctcgtcgtc gaggaacatg aaggccgacc ctatggctat gcctggcgag gccggtaggc
     2641 cctggagcgt tctcatagta cgggcatggt agtccaaaag gattcgactg tcaaactgtg
     2701 ggcgtcgtca acggagcacc gacatcgggt tgagcgtcct gccgttcttg ctgacggtga
     2761 agtgcaggtg cgggccggtg ctgtagccgg tgctgccgac ggccccgatg accgtggtgg
     2821 tcgtcacgta
//
LOCUS       DAOOLN010000109         2822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_43579_length_2822_cov_3.067944, whole genome shotgun sequence.
ACCESSION   DAOOLN010000109 DAOOLN010000000
VERSION     DAOOLN010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43579_length_2822_cov_3.067944"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..698)
                     /locus_tag="PLW80_02100"
     CDS             complement(15..698)
                     /locus_tag="PLW80_02100"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HPB65321.1"
                     /translation="MACLGRARRGLSYRQGKRALAAHDAAAAVKLFRAAVASCPADER
                     GLLASYLYWLSVALRRLGNDGLAIKALASAQRLEPRGHSRSVYARVANQYGMPKAASA
                     EHDDYRAFCSIQLRKYLSGAPGGRFDSQEEIDAVLSIIAGAWLRLGEARPFADASCND
                     KLRAFRDVSIEYPTLRERGLGMETRVIPVDFYRGKALKANDRCTCGSGLPYGKCCGRV
                     RLPYEAERD"
     gene            complement(730..>2822)
                     /gene="glgX"
                     /locus_tag="PLW80_02105"
     CDS             complement(730..>2822)
                     /gene="glgX"
                     /locus_tag="PLW80_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HPB65322.1"
                     /translation="GVNFALFSRHATSVRLCLFAKADDGEPILDHRLDPVQNRTGDIW
                     HIHVDGLRPGALYAWRADGPFMPERGYRFNPNKLLVDPYAKALSGDFEWDMKAALGYD
                     ADDPRADLSFSRIDDAATMPKCVVVDDDEFDWEGDKPLNLPLRHCVIYETHVRGLSAH
                     ASAGSSHPGTYLGLVEAIPYFKRLGVTSLELLPVQEFDHHENTRKDPRTGKRLSQYWG
                     YSTLAFFAPKGRYASSSRSGQQVAEFKSMVKALHRAGIEVILDVVFNHTGEGSELGPT
                     VSFRGLDNPVWYMLDEDPRRYKNYSGCGNTLNCNHPVVRGFIVDCLHYWVADMHVDGF
                     RFDLGSILGRDQKGRLLENPPVIEQIAEDPLLRQTKIIAEAWDAGGAYQVGGFPGGRW
                     AEWNDRYRDDVRRFWRGDEGAVAAIATRLTGSSDLYLRDGRKPFHSINYITSHDGFTL
                     ADLVSYNGKHNERNGERGLDGHDSNFSYNHGHEGPSRAPAVESLRVRQQKNFLATLLL
                     SIGTPMLLGGDEFGRTQRGNNNAYCQDNETGWYDWSLAVEHDELLRFTARLIALRLAH
                     PAFRRPEFFTGKDANYNALPDIAWYAPDGSAPDWASMPSALAARLDGSKADIVADRDD
                     DDFLLLLNATAGAIDFRVGAPQAGWAWYRAVDTNLSSPYDAAEPGCEPPVDHETPYRL
                     GPKSLALLMAKPPT"
BASE COUNT          459 a          849 c         1014 g          500 t
ORIGIN      
        1 actatgtatc gtttctagtc acgttcggct tcgtacggaa gccgaacgcg gccgcagcac
       61 ttgccatagg gcaggcccga gccgcaggtg caacggtcgt tggccttaag agccttgccg
      121 cggtagaaat cgaccggaat gacgcgggtc tccatgccga ggcctcgctc gcgtagggtc
      181 gggtactcga tgctaacatc cctgaaggcc cgtagcttat cgttgcagct cgcatcggcg
      241 aaaggcctgg cttcgccgag gcgtagccag gccccggcta tgatcgagag tacggcgtcg
      301 atctcctcct ggctgtcgaa gcggccgccc ggtgctccgg acaggtactt acgcagctgg
      361 atggagcaga aggcgcggta gtcgtcatgc tcggcgctgg cggccttggg catgccgtac
      421 tgattggcga ccctcgcata gacggaccgt gaatggccgc gaggctccag gcgctgggcg
      481 ctggcgaggg ccttgatggc cagaccgtca ttaccgagcc ggcgcagggc taccgatagc
      541 caatagaggt agctggcgag caacccgcgc tcatcggccg ggcaggaagc cacggccgcg
      601 cggaacagct taacggcggc agccgcgtcg tgcgcggcaa gcgcccgttt tccctgtcgg
      661 taggataatc ctcgtcgcgc acgccctaag caagccatga aaagaaaata cgcataatcc
      721 gcgcgccgat caagtcggcg gcttggccat gagcagggcc aggctcttgg ggccgaggcg
      781 atagggcgtt tcatggtcta cgggcggctc gcatcccggt tccgccgcgt cgtagggtga
      841 cgacaggttc gtgtcgacgg cgcggtacca ggcccagccc gcctgcggcg cgccgacgcg
      901 gaagtcgatg gcgccggccg tcgcgttcag gaggagaagg aagtcgtcgt cgtcgcggtc
      961 ggcgacaata tcggctttgg agccgtcgag gcgagcggcg agggccgacg gcatagaagc
     1021 ccagtcgggc gcggaaccgt caggcgcgta ccaggcgatg tccggcaggg cgttgtagtt
     1081 ggcgtccttg ccggtgaaga actcggggcg cctgaacgcc ggatgggcta gccggagggc
     1141 tatgagcctg gccgtgaagc gtagcagctc atcgtgctcg acggccaggc tccagtcgta
     1201 ccagccggtt tcgttatcct ggcagtaggc gttgttgttg ccgcgctggg tcctgccgaa
     1261 ctcgtcgccg ccgagcagca tcggggtgcc gatggacagg agcagtgtgg ccaggaagtt
     1321 cttctgctgg cgcacgcgca gcgattcgac ggccggggcg cggctcgggc cctcgtggcc
     1381 gtggttgtag ctgaagttgg agtcgtggcc gtcgaggccg cgctcgccgt tccgctcgtt
     1441 gtgcttgccg ttgtagctga ccaggtcggc cagggtgaag ccgtcgtggc tggtgatgta
     1501 gttgatcgaa tggaacggct tacggccatc gcgcagatag aggtcgctcg agccggttag
     1561 cctagtggcg atcgcggcca cggcgccttc gtcgccgcgc cagaagcgcc gcacgtcgtc
     1621 tcggtagcgg tcgttccact cggcccagcg ccctcccgga aagccgccga cctgataggc
     1681 gccgcccgcg tcccaggctt ccgcgatgat cttggtctgc ctgagtagcg ggtcctcggc
     1741 tatctgctcg atgaccgggg ggttctcgag caggcggccc ttctgatcgc gcccgagtat
     1801 cgagccgagg tcgaaacgga agccgtcgac gtgcatgtcc gcgacccagt agtggagaca
     1861 gtcgacgatg aagcctcgga ccacgggatg gttgcagttc agcgtgttgc cgcagcccga
     1921 gtagttcttg tagcggcgcg gatcctcgtc gagcatgtac cagaccgggt tgtcgaggcc
     1981 gcggaaggac acggtcgggc cgagctcgct accctcgccg gtatggttga acaccacgtc
     2041 gagtatcacc tcgatgccgg ctcggtgcag tgccttgacc atggacttga actcggccac
     2101 ctgctggccg gagcggctcg acgaggcgta gcggcccttg ggcgcgaaga aggcgagggt
     2161 gctgtagccc cagtactggg atagccgctt gcccgtgcgc gggtcctttc gagtgttttc
     2221 atggtggtcg aattcctgga ccggcaacaa ctcgagactg gtcacgccga ggcgtttgaa
     2281 gtaggggatg gcctcgacga ggcccaagta ggtgcccggg tggctcgagc cggcgctcgc
     2341 atgggcggac aggccgcgta catgggtctc gtagatcacg cagtggcgca ggggcaggtt
     2401 gagcggcttg tcgccctccc agtcgaactc gtcgtcgtcg accacgacgc atttgggcat
     2461 cgtggccgcg tcgtcgatcc tggaaaagga caggtcggcg cgcgggtcgt ccgcgtcata
     2521 gccaagggcc gccttcatgt cccactcgaa gtcaccggac agcgccttgg cgtaggggtc
     2581 gacgagtagc ttgttagggt tgaagcgata gccgcgttcc ggcatgaagg gaccgtcggc
     2641 ccgccacgca tagagggcgc cgggccgcag gccgtcgacg tggatgtgcc agatgtcgcc
     2701 ggtgcggttc tggaccgggt ccaggcgatg gtcgaggatg ggctccccgt cgtcggcctt
     2761 ggcgaagagg cagaggcgta cgctcgtcgc gtgacgcgag aacagcgcga agttgacgcc
     2821 gc
//
LOCUS       DAOOLN010000110         2815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_43722_length_2815_cov_3.048913, whole genome shotgun sequence.
ACCESSION   DAOOLN010000110 DAOOLN010000000
VERSION     DAOOLN010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43722_length_2815_cov_3.048913"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2008)
                     /gene="purD"
                     /locus_tag="PLW80_02110"
     CDS             complement(<1..2008)
                     /gene="purD"
                     /locus_tag="PLW80_02110"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPB65323.1"
                     /translation="MKILLIGSGGREHAIAEALSRSPAGGELVIAPGNPGTAALGRNV
                     PVSLADVAAIAALAEAEAADLVVVGPEAPLALGLADELAARGIACFGPSRAAAALESS
                     KAFAKATMASLGVPTADYRVCTDFDEAAAFVRAAPWPLVVKASGLASGKGVVLPDSAG
                     EAVGALHAMKRGGRLGDAAREVVVEERLEGEELSLMAFCDGSRLALMPGARDHKRLLD
                     GDRGPNTGGMGAYAPACTTELAAEYARLTMEPLVDLMRARGTPFVGVLYAGLIVTPSG
                     PKVLEYNCRFGDPEAQAVLALFAGDLAATMAACAAGDLRKAPPSWKTGAAACVVMAGD
                     GYPERPVGGAVLSGLEPVADGRASVARVLHANTRLEGGRLVSGGGRVLSVVAAGDKLP
                     EAVAAAYAKADEVSFPGARYRRDIGARELARLAADKPRRSSAYASSGVDIDAGNEAVR
                     RMSAAVKATYSEAVLAGIGSFGGLYDATSIKTMAQPVLVASTDGVGTKVKLAAQAGSY
                     ASIGQDIVNHCIDDILVQGARPLMFLDYFASSRLDPAMVAEAVGGMAAACRAAGCALI
                     GGETAEMPGVYRDGEFDVAGTILGVVERGSILPAAGVGPGDALVGFASSGPHTNGYSL
                     ARKAFESVDLSTVYPELGRPLGEALLAPHRSYLPLLWPVLSGSPG"
     gene            complement(2005..>2815)
                     /locus_tag="PLW80_02115"
     CDS             complement(2005..>2815)
                     /locus_tag="PLW80_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPB65324.1"
                     /translation="SESCALLTLGCVGVEDIGPGRIDELTARGPLDCGGVDAEPGASC
                     VFEYVYFSRPDSVWNGVPVHEARKRFGAALALEAPANADLVIAVPDSSISAAIGYAQA
                     SGLPYDEGFAKSRYIGRTFIHPTQGLRDQGVALKFNAMPAAVSGRRVVVVDDSIVRGT
                     TTGPLVRLLREAGAAEIHVRIACPPLRHPCYMGVDMGPADGLVARRRSIEGIRGTVGA
                     DSLAYLSLDGMTRALAEAGAGAGTGFCSACFSGDYPIDVGGAAGKNGFEVI"
BASE COUNT          402 a         1009 c         1022 g          382 t
ORIGIN      
        1 ggcccggcga gccggacagg accggccaca ggagcggcag gtacgagcga tgcggcgcga
       61 gcagggcctc gccgagcggc cgaccgagct ccgggtagac ggtcgacagg tcgacgctct
      121 cgaaggcctt gcgcgccagc gagtagccgt tcgtgtgggg gccggatgaa gcgaacccga
      181 cgagggcgtc gccgggtccg acgccggcgg ccggcagtat ggagccgcgc tcgacgacgc
      241 cgagtatcgt gccggccacg tcgaactcgc cgtcccggta gacgccgggc atctccgcgg
      301 tttcgccgcc tatgagcgcg caaccggcgg cccgacaagc ggccgccatg ccgccgacgg
      361 cttcggcgac catcgcgggg tcgagcctgg agctggcgaa gtagtcgagg aacatgagcg
      421 ggcgggcgcc ctgcacgagt atgtcgtcga tgcagtggtt aacgatgtcc tggcctatgg
      481 aggcgtagga gccggcctgg gcggccagct tgaccttggt gccgacgccg tccgtcgagg
      541 cgaccagtac gggctgcgcc atggtcttta tgctcgtagc gtcgtagagc ccgccgaagg
      601 agccgatgcc ggcgagtacc gcctccgagt aggtggcctt gaccgcggcc gacatgcgcc
      661 tgactgcctc gttgccggcg tcgatgtcga cgccggagct agcgtacgct gaactccgac
      721 gaggcttgtc cgcggcgagc ctggcgagtt cccgggcgcc gatgtcgcgg cgatagcgag
      781 cgccggggaa tgatacttcg tcggccttgg cgtaggccgc ggctacggcc tcgggcagct
      841 tgtcgccggc cgcgacgacg gataggacgc gcccgccgcc ggagacgagg cgcccgccct
      901 ccaacctcgt attggcgtgc aggacccggg cgaccgaagc gcgtccgtcc gccaccggct
      961 cgaggccgga cagtacggct ccgccgaccg gccgctcggg gtacccgtcg ccggccatca
     1021 cgacgcaggc ggccgctccc gttttccagg acggtggcgc cttccgcagg tcgccggccg
     1081 cgcaagccgc catggtggcg gccaaatcgc cggcgaacag cgccagcacg gcctgggcct
     1141 ccgggtcgcc gaagcggcaa ttgtattcga gcaccttcgg gcccgagggg gtgacgatga
     1201 gccctgcgta caggacgccg acgaacggcg tcccccgcgc gcgcattaag tcgaccagcg
     1261 gctccatcgt cagcctggcg tactcggcgg ccagctccgt cgtacaggct ggcgcgtagg
     1321 cgcccatgcc gccggtattc ggccctcggt ccccatccag gagccgctta tggtcccgcg
     1381 cgccgggcat gagcgccagg cgcgacccgt cgcagaaggc catcaggctg agctcctcgc
     1441 cctcgagtcg ctcctcgacc acgacctccc gggcggcgtc gccgaggcgc ccgccgcgct
     1501 tcatcgcgtg cagggcgccg accgcctcgc cggccgagtc cggcaggacc acgcccttgc
     1561 cggaggccag gccggaggcc ttgacgacca gcggccaggg cgcggcccgg acgaaggcgg
     1621 ccgcctcgtc gaagtcggta caaaccctgt agtcggccgt cggcacgccg aggctggcca
     1681 tcgtcgcctt ggcgaaggcc ttggacgact cgagcgccgc agccgcccgg ctcgggccga
     1741 agcaggctat gccgcgcgcc gcgagctcgt ccgccagccc cagcgccagc ggggcctccg
     1801 gcccgacgac gaccaggtcg gccgcctcgg cctcggcgag cgcggcgatc gccgctacgt
     1861 cggcgagcga taccgggacg ttccgcccga gcgccgcggt gcccgggttg ccgggagcga
     1921 tgacgagctc gccgccagcc ggcgagcggc tcagcgcctc ggcgatcgcg tgctcgcggc
     1981 cgccagagcc tattaggagt atcttcatat cacctcgaat ccgttcttgc cggcggcgcc
     2041 gccgacgtct atcgggtagt cgcccgagaa gcaggcggag cagaagccgg ttccggcccc
     2101 cgctcccgcc tcggcgagcg ccctcgtcat cccgtccagc gataggtacg cgaggctgtc
     2161 cgccccgacg gttccgcgta tgccttctat cgatcgcctg cgcgcgacga gtccgtcagc
     2221 ggggcccatg tcgacgccca tgtagcaggg gtgccgtaga ggcgggcagg cgatgcgtac
     2281 gtggatctcc gcggcccccg cctcgcggag cagcctgacg aggggtcccg tcgtcgtgcc
     2341 gcgcacgatg ctgtcgtcga ccacgacgac gcgcctgccg ctgaccgcgg caggcatcgc
     2401 gttgaacttg agcgccacgc cctggtcccg taggccctgg gtcgggtgga tgaaggtccg
     2461 gccgatgtag cggctcttgg cgaagccctc gtcgtagggc aggcccgagg cctgggcgta
     2521 gcctatggcg gccgagatcg acgagtcggg tacggcaatc acgaggtcgg cgtttgcggg
     2581 cgcctcgagc gcgagcgcgg cgccgaagcg cttgcgggcc tcgtggaccg gcacgccatt
     2641 ccagacgctg tccggccgcg agaagtagac gtactcgaac acgcacgagg cgcccggctc
     2701 ggcgtcgacg ccgccgcagt cgagcgggcc gcgggcggtc agctcgtcga tgcgaccggg
     2761 tcctatgtcc tcgacgccga cgcaaccaag agtcaagagg gcgcagctct cgctg
//
LOCUS       DAOOLN010000111         2805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_43956_length_2805_cov_3.329091, whole genome shotgun sequence.
ACCESSION   DAOOLN010000111 DAOOLN010000000
VERSION     DAOOLN010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43956_length_2805_cov_3.329091"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PLW80_02120"
     CDS             <1..581
                     /locus_tag="PLW80_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65325.1"
                     /translation="APAPRALAGALGELAARRASEDFPPAAVKEPVRAMLRAGGFKPA
                     GRQKPASEYLARAARDGVFPSINGPVDCNNLLSLETGLPISLLDADELGGSALIRVCG
                     AGEAYVFNASGQEMDLAGLLCVCAADGSPLGNPVKDSMRGKLKDGTRRLAGFIYAPLG
                     LYSPTSLERVGERFAALLREHCGATARVAGTV"
     gene            584..1561
                     /locus_tag="PLW80_02125"
     CDS             584..1561
                     /locus_tag="PLW80_02125"
                     /inference="COORDINATES: protein motif:HMM:NF015889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HPB65326.1"
                     /translation="MGAFAQILVLLAFMAAGALIHRVRLSPPAKVTDRLIKLVLWALL
                     LVMGFRLGNARELRDRLGEIGLLAVGTAFATLAGTIAAIRAAYSLLETLRTRRGGHRA
                     GAPGARPDGGSGPVTDGSPRLGWEHFKGPSVLLVVVIVGFVTGLALPELALDYGSVTG
                     WVLNALLFMIGLQFAQSGISLRSAFVRADTALVPIATMVGSLAGGLVVAAAFSISAGK
                     ALSLAAGFGWYSLSGVLLSDLGDPVLGSAAFLANMARESMALLLIPLLSRSKRPYTAV
                     GAGGATAMDVTLPLIEQCLGPDSVPVSFTSGAILSLLVPVLVPLFYGLG"
     gene            complement(1594..2385)
                     /locus_tag="PLW80_02130"
     CDS             complement(1594..2385)
                     /locus_tag="PLW80_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65327.1"
                     /translation="MNQDQVKELLLRVEDCKTEFSVVFTGKKSGMVNGLYKPLTREIL
                     IHNKNFENDGQLIYTAIHEYAHHIHCERGGFVPGARSHTNEFWAIFHELLEKAEGLGL
                     YENSFDSDPDFVEMTGRIRALLPENGRVMLEFGKLVVEAEALCRKHLVRFEDYLDRAI
                     GIPRTSAAAAMKAYSFQVPAEIGWDAMKVVSGIRQSEVRGQAIEAFKSGKSPDTVKAM
                     VRRDKPADDPSERLQKERERLERTIKNLQDRLTMVESELSKMAAD"
     gene            2553..2780
                     /locus_tag="PLW80_02135"
     CDS             2553..2780
                     /locus_tag="PLW80_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65328.1"
                     /translation="MAKRTNYSFEKRQKDLAKKKKREDKLKKKQERKASGVQTIVQID
                     EYGNVVEIEVDADGTPIVDSDDDDDDDSEDD"
BASE COUNT          421 a          948 c          934 g          502 t
ORIGIN      
        1 tggccccggc gccgcgcgcc ctcgcgggcg cgctcggcga gctggccgct cgccgagcct
       61 cggaggactt cccgccggcc gccgtcaagg agccggtgcg cgcgatgctg cgcgcggggg
      121 gcttcaagcc ggccggtcgc cagaagcctg ccagcgagta cctggcgcgg gccgcccgcg
      181 acggcgtctt cccgtcgatc aacgggccgg tcgactgcaa caacctgctg tcgctcgaga
      241 ccgggctgcc catcagcctg ctcgacgccg acgaactcgg cggctcggcc ctgatccgcg
      301 tctgcggggc cggcgaggcc tacgtgttca acgcctccgg ccaggagatg gacttggccg
      361 ggctcctgtg cgtctgcgcc gccgacggct cgccgctcgg caacccggtc aaggactcga
      421 tgcgcggcaa gctcaaggac ggcacgaggc ggctcgccgg ctttatctac gcgcctcttg
      481 gcttatactc gcccacgagc ctcgaacggg tgggggagcg cttcgcggcc ctgctccgcg
      541 agcattgcgg cgcgacggcc agagtcgccg ggacggtcta ggcatgggcg ccttcgcgca
      601 gatactcgtc ctcctggcct tcatggccgc cggcgcgctg atccaccgcg tcaggctatc
      661 gccgccggcc aaggtgaccg accgtttgat caagctggta ctctgggccc tgctgctcgt
      721 gatgggcttc aggctcggga acgcgcgcga gctacgcgac cggctcggcg agataggctt
      781 gttggcggtc ggcacggcgt tcgccacgct cgccggtacg atagcggcga tccgcgcggc
      841 ctactcgctt ctcgagacgc tgcgaacccg tcgaggcggc catcgcgccg gcgcgcccgg
      901 cgcgcggccc gacggcggct cagggccggt gaccgacggg tcgccgcggc tgggctggga
      961 acatttcaag gggccgtcgg tcctgctcgt cgtcgtcatc gtcggtttcg tgaccgggct
     1021 cgccctaccg gaactggcgc tcgactacgg ctcggtgacc ggctgggtgc tcaacgcgct
     1081 cctgttcatg atagggctcc agttcgccca gtccggcata tcgctgcgct ccgccttcgt
     1141 gcgggccgac accgcgctcg tgccgatagc tacgatggtc ggcagcctcg ccggtggact
     1201 cgtcgtcgcg gcggccttct cgatcagcgc cggcaaggcc ctctcactcg cggccggctt
     1261 cggctggtat agcctctcgg gcgtgctgct atccgacctc ggcgacccgg tcctcggctc
     1321 ggcggccttc ctcgccaaca tggcccgcga gtcgatggcc ctcctcctga taccgctcct
     1381 gtcgcggtcc aagcggccgt acacggcggt cggggccggg ggggccaccg cgatggacgt
     1441 gacgctcccg ctcatcgagc agtgcctcgg cccggactcg gtgccggtta gtttcacgag
     1501 tggggcgata ctatcgctcc tcgtgccggt gctggtgccg ttgttctacg gcctcgggta
     1561 gcgtcccctg gctgcggctt agtcggccga ggcctagtcg gccgccatct tcgacagctc
     1621 ggactcgacc atggtcaggc ggtcctgcag gttcttgatg gtccgctcga gccgctcgcg
     1681 ctccttctgc aggcgctccg acgggtcgtc ggcgggcttg tcccgcctga ccatggcctt
     1741 gacggtgtcg gggctcttgc ccgacttgaa cgcctcgata gcctggccgc gaacctcgga
     1801 ctgcctgatg ccggagacga ccttcatcgc gtcccagcct atctcggcgg gtacctggaa
     1861 gctataggcc ttcatcgcgg cggcggcgct cgtcctcggg ataccgatgg cgcggtccag
     1921 gtaatcctcg aagcggacca ggtgcttacg gcacagcgcc tccgcttcga cgacgagctt
     1981 gccgaactcg agcatcacgc ggccattctc cggcagaagg gccctgatgc gcccggtcat
     2041 ctcgacgaag tccgggtcgg agtcgaagct gttctcgtac aggcccaggc cctcggcctt
     2101 ctcgagcagc tcgtggaaga tggcccagaa ctcgttcgtg tgcgacctgg cgccgggtac
     2161 gaagccgcct cgctcgcagt ggatatggtg ggcgtactcg tggatggccg tatagatcag
     2221 ctggccgtcg ttctcgaagt tcttgttatg gataagtatc tccctggtca ggggcttgta
     2281 gaggccgttg accataccgc tcttcttgcc cgtgaacacg acgctgaact ccgtcttgca
     2341 gtcctcgacc ctgagcaaga gttccttgac ctgatcctgg ttcatcgatg gcctccgccg
     2401 cgcactgtac tacggcgccg ctcggggcgg caagcgactc agtatcgcga ccggcataga
     2461 ccatgaggta aaaaaggttt aggatagtag ctctcaggcg tcggttcgaa gcgccgagcc
     2521 ccaaccatcg ttcaatcatg aaagggatat ccgtggcgaa aaggaccaat tattcgttcg
     2581 agaagcgcca gaaggatctg gctaagaaga agaagcgcga ggataagctc aagaagaagc
     2641 aggagcgcaa agcctccggc gtccagacca tcgtacagat agacgagtac ggcaacgtgg
     2701 tcgagatcga ggtggacgcg gacggcaccc ccatcgtcga ctccgacgac gacgacgacg
     2761 acgattcgga agacgactag acgcgggagc cgttccggcc ggata
//
LOCUS       DAOOLN010000112         2798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_44120_length_2798_cov_3.186657, whole genome shotgun sequence.
ACCESSION   DAOOLN010000112 DAOOLN010000000
VERSION     DAOOLN010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44120_length_2798_cov_3.186657"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="PLW80_02140"
     CDS             <1..1122
                     /locus_tag="PLW80_02140"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPB65329.1"
                     /translation="DRLFENEAIDVTTELLSVLPDACGRLLVSASLPNRTVDRSAAWF
                     RNPLRLIVDPSEALRTSIEHWCFHSVSRSKLDFLRRFEAAVKPERCLLFASSNASIFN
                     IQKRLDYLGFPAVVLKSDKEGEERKNALADFTSGAARWMITTDLGARGLDLPDVSHVI
                     SFDLPEEPTVYMHRAGRTGRAGKRGVSVTLADLVELKRASKIAVRYGFSFVCKILDSG
                     IVHDIEPENFFAIAEEEEAARKDVKLEGTKRPESGRRRRPVGTSVGERPRRPPAEGGY
                     RSAMEPNRQPARQPSQPPRRDRPRQSPKPGRPQPLAGQQGRSPVAAPAGSPNGTPNNP
                     PNGTPNGNPNGNPNGNQPAKRKHRRRKGGPKDAQKPSAE"
     gene            complement(1134..>2798)
                     /locus_tag="PLW80_02145"
     CDS             complement(1134..>2798)
                     /locus_tag="PLW80_02145"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HPB65330.1"
                     /translation="PPGYCSAQLIPYLGNKRALLPRLYPVFKRLTSGRVGPLSFADAF
                     AGSGAVSRLARAMGLRVASNDWEPYSQAIGHCWLALSPTDLDAAFGGGQGLASFLDDW
                     NSMHPLSGVPYGGAAEPYIARWYAPERTDAPRLGEERLFYTAENATFIDRVRERVESE
                     YPSPEPLSPGDVRRRVAMGALLLEAAVHTNTSGVFKAYHRGFGGHGKDALGRILGRME
                     LEAPVLPDAPPAEVYGLDAAAFMAGKSFDIAYYDPPYNQHQYGPNYHLLNTILVWDRE
                     PRPLASSERHSTKAGIPVDWKERRSPFCVRREAAGAIRSLLDATDAGSIVFSWNADGH
                     LSGEDMAALLAPRGRLEIIALDYIAYRGGRQSASRSERSREYLFVVDTRAEATDEDLA
                     RRSLADLAAVDEALRSSYDPERVRSVFFGSEAPWLEAAAFFGGGMRRAADDAGQLLRA
                     MPDDRRGPFCEALAACACSSVSDDLDALLATGNAAIGAGDRRAARIAGSKALRYVRKL
                     AHEKYADEFRRYLGAFAAMAEACGDEATSVGLVELAQLVERRKHGD"
BASE COUNT          474 a          977 c          929 g          418 t
ORIGIN      
        1 gaccgcctgt tcgagaacga ggccatcgac gtcaccaccg agctcttatc ggttctgccc
       61 gacgcctgcg ggcggctcct cgtctcggca tccctgccga accgcaccgt cgaccggtcg
      121 gcggcctggt tccggaaccc gctcaggctc atcgtcgacc cctccgaagc gctacgcacg
      181 agcattgagc actggtgctt ccactccgtg tcccgatcga agctcgactt cctccggcgc
      241 ttcgaggcgg cggtcaagcc cgagcgctgt ctgctattcg cctcgtcgaa cgcgtccatc
      301 ttcaacatcc agaaacggct ggactacctg ggctttcccg cggttgtcct caagtccgac
      361 aaggagggcg aggaacgcaa gaacgccctg gccgacttca cgagcggagc cgccaggtgg
      421 atgatcacca ccgacctagg cgctcgcggc ctcgacctgc cggacgtgtc ccacgtcatc
      481 agcttcgacc tgcccgagga gccgaccgtc tacatgcatc gcgccggccg aacgggccgg
      541 gccggcaagc gtggcgtatc ggtgacgctc gccgacctgg tcgagctgaa gcgagcgtcc
      601 aagatagccg tgcgctacgg cttctcgttc gtctgcaaga tactcgactc cggcatcgtg
      661 cacgacattg aaccggagaa ttttttcgct atcgcggagg aagaggaagc cgccaggaag
      721 gacgtcaaac tcgagggcac gaagcgcccc gagtccggca ggcgccggcg tccggtcggt
      781 acctcagtgg gcgagcggcc tcgccgcccg cccgcggaag gcggctatcg ctcggccatg
      841 gagccgaacc gccagcctgc gcgtcaaccg tctcagccgc ctaggcgcga tcggccgcgg
      901 cagtccccta agccaggtcg accgcagccc cttgccggcc agcagggccg ctcgcctgtg
      961 gccgcccccg ctgggtcacc gaacgggacg ccgaataatc ccccaaacgg cacccccaac
     1021 gggaacccca acgggaaccc caacgggaac cagcccgcca agcgtaagca tcgtcgccgc
     1081 aagggcggcc ccaaggacgc tcagaagccg agcgccgaat agcgctaccc ggttcagtcg
     1141 ccgtgcttcc ggcgctcgac gagctgggcg agttcgacga ggccgaccga cgtcgcttcg
     1201 tcgccgcagg cctccgccat cgccgcaaac gcgccgaggt atcgtcggaa ttcgtcggcg
     1261 tacttttcat gagccagctt acgcacgtaa cgcagggcct tcgacccggc gatcctggcc
     1321 gcgcgacggt ctccggcgcc gatagccgcg ttaccggtcg cgagcaaggc gtccaggtcg
     1381 tcggagaccg aggagcaggc gcaagcggcc agcgcttcgc agaacggtcc gcgcctatcg
     1441 tcaggcatgg cgcgcagaag ctggccggcg tcgtcggccg cccgcctcat gccgcccccg
     1501 aagaacgcgg ccgcctcgag ccagggcgcc tccgatccga agaacacgga gcggacgcgc
     1561 tcggggtcat agctagaccg cagggcctcg tccacagccg ccaggtcggc tagcgaccgc
     1621 ctggccaggt cctcatcggt ggcctcggcg cgcgtatcga cgacgaacag gtactcgcgg
     1681 ctccgctcgc tcctggacgc cgactgtcgc ccgccgcgat acgcaatgta gtcgagggcg
     1741 atgatctcca gtcggccgcg tggggctaga agggccgcca tatcctcgcc gctcaggtgg
     1801 ccgtcagcat tccagctgaa gacgatggag ccggcgtcgg tggcatcgag cagggatcgt
     1861 attgcgccgg ccgcctcgcg ccgtacgcag aacggcgagc gccgctcctt ccaatcgacc
     1921 gggatgcctg ccttggtaga atgacgttcc gacgaggcca agggccgagg ctcgcggtcc
     1981 cagacgagta tggtgttgag caggtggtag ttcggaccgt actggtgctg gttgtacggc
     2041 gggtcgtaat aggcgatatc gaaactcttt cccgccatga aggccgcggc gtcgagcccg
     2101 tagacctcgg ccggaggggc gtcgggcaac acgggggcct cgagctccat gcgtccgagt
     2161 atacgcccga gggcgtcctt gccgtggcca ccgaacccgc gatggtaggc cttgaagacg
     2221 ccggaggtat tcgtatgcac ggcggcctcg aggaggaggg cgcccatcgc gacacggcgg
     2281 cggacatcgc ctggcgatag aggctccggc gatgggtact cagactcgac gcgctcgcgg
     2341 acgcggtcta tgaacgtggc gttctccgcg gtatagaaga gccgctcttc tcccagcctc
     2401 ggcgcgtcgg tgcgttccgg ggcataccag cgcgctatgt acggctcggc cgcgcctccg
     2461 tacggtacgc ccgacagggg gtgcatcgag ttccagtcgt ccaagaacga ggcgaggccc
     2521 tggccgccgc cgaaggccgc gtccaggtcg gtcgggctca gggctagcca gcaatggcct
     2581 atggcctggc tgtacggctc ccagtcgttg gaggccaccc tgaggcccat ggccctcgct
     2641 agcctcgaga ccgccccgct acctgcgaac gcgtcggcga acgacaacgg ccccacccgg
     2701 ccagaggtca gccgcttgaa gaccgggtac agccgcggga ggagggcgcg cttgttgccg
     2761 aggtacggta tcagctgcgc cgagcagtag ccgggcgg
//
LOCUS       DAOOLN010000113         2785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_44409_length_2785_cov_3.190476, whole genome shotgun sequence.
ACCESSION   DAOOLN010000113 DAOOLN010000000
VERSION     DAOOLN010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44409_length_2785_cov_3.190476"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(45..176)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            243..1223
                     /locus_tag="PLW80_02150"
     CDS             243..1223
                     /locus_tag="PLW80_02150"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPB65331.1"
                     /translation="MELLSTSARDLERAAEAIRAGLLVAIPTETVYGLGADAWNRVAL
                     ARVFEAKRRPSFDPLIVHVAEPAGASEVADLSARYAATLAERFWPGPLTMVLPKRERV
                     PDLATSGLPTVAVRCPANAVAREIIRRSGVPVAAPSANPFGYLSPTRAEHVVAQLGDR
                     VDFIVDGGRCPVGVESTVLDLSTDEPLVLRPGGLPVELIAEVIPGVRVYDRATTSPSA
                     PGQLPSHYAPSRPLYLVPHGGVAGIEPRGRAAALCFGSDAAKSAERGERWSRIIDLSP
                     SMDPVEAAAALFDALHELDAAGFDELWAERLPDEGLGRAVNDRLYKASVK"
     gene            1322..1693
                     /locus_tag="PLW80_02155"
     CDS             1322..1693
                     /locus_tag="PLW80_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65332.1"
                     /translation="MDVRISNQLPLAYAYSAGASGRISVPVSPSQLLYSNFKNVSGIA
                     SAGASAYTLDKLKILDTIIERLRSAKNQPRVEREATGLSDERIDALIEQYGAQLHNAL
                     GAGPYAKPTGVVPGMLVSIAA"
     gene            complement(1855..>2785)
                     /locus_tag="PLW80_02160"
     CDS             complement(1855..>2785)
                     /locus_tag="PLW80_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPB65333.1"
                     /translation="GSFVDLGGYIDSRLHEAYGQRVDPWYRLVAAYRLGEPLVEPLAV
                     PGTEPAPTESGGTASADDEARRPVSPDEYALALTRFGLGYRVRAELGDEFRSVSENAV
                     RTIAAELDAAGRHDQAYRLIATLFWRTGFEPTRSDAELYWPRPYPEAFSPAIEATGLD
                     PHLLYGLARSESAFDPKAVSKSGAVGLIQLMPATAAETAERLDLDDYELTDPLDNVTI
                     GSSYLARLLDRLAGRVLPAVFSYNGGPTRFRRWEAEYGALPLDLLLEALSYAETRQYG
                     RNVGHAALSYAALYGEVDLRAYMAWLLGEGPRP"
BASE COUNT          443 a          979 c          931 g          432 t
ORIGIN      
        1 cctggccgcc ggactatcgc tttacgcctg taccacgaag cgttccacga agattacatc
       61 ggcgcgggta tcctggctcg ccggcgcccg gagagggcgc ggcctacagt tacgggctag
      121 ctccggattc tgaccggatt cccccgcgtc gcgatacctc ggtactatag gtaccgcgct
      181 gccgggggtc aaggggaccg ccaccgattg ccccgagaag gcgcggggcg tataatccgc
      241 gcatggaact cttgtcaacc tccgcgcggg acctcgagcg tgccgccgag gccatacggg
      301 ccgggctcct cgtagccata cctaccgaga ccgtctacgg cctcggcgcc gacgcgtgga
      361 accgggtcgc gctggcccgc gtcttcgagg ccaagcgccg cccgagcttc gacccgctga
      421 tcgtccacgt agccgagccg gccggcgcct cggaggtggc cgacctgtcg gcgcgctacg
      481 ccgcaaccct cgccgagcgc ttctggcccg gcccgctgac gatggtgctg cccaagcgcg
      541 agcgggtgcc cgacctcgcg acgagcggcc tgccgaccgt cgccgtccgc tgtcccgcca
      601 acgcggtggc ccgcgagatc atccgccgct caggcgtgcc ggtcgccgcg ccgagcgcca
      661 accccttcgg ctacctgtct ccgacccgag ccgagcacgt cgtcgcgcag ctcggcgatc
      721 gcgtcgactt catcgtcgac ggcggccgct gcccggtcgg cgtcgagagc accgtgctcg
      781 acctgagtac cgacgagccg ctcgtgctcc ggcccggcgg cctgcccgtc gagctgatag
      841 ccgaggtgat ccccggcgtg cgcgtctacg accgagccac gaccagcccg tccgcgccgg
      901 gccagctgcc gagccactac gcgccttcgc ggcccctgta cctcgttccc cacggcggcg
      961 tcgccggtat cgagccccgc ggtcgggccg ccgcgctctg cttcggctcg gacgcggcga
     1021 agtcggcaga gcgcggcgag cgctggtcca ggattatcga cctgtcgccg tcgatggacc
     1081 cggtcgaggc ggccgcggcc ctgttcgacg cgctccacga attggacgcc gccggcttcg
     1141 acgagctgtg ggccgagcgc ctgcccgacg aggggctcgg ccgggcggtc aacgaccggc
     1201 tgtacaaggc ctcggtcaag tagggccgtc tatcgtcgcc gttcgtcagc taacgctaat
     1261 actcgcccct cgccgctccg acaatatagg tagaaagtcg gtatcgagta cggagcggat
     1321 catggacgtg aggatttcga atcagcttcc attagcgtac gcctatagcg cgggggcgag
     1381 cggcaggata tccgtaccgg tctccccgtc acagctcctg tactccaact tcaagaacgt
     1441 atccggcatc gcgagcgctg gcgcttccgc ctacaccctg gacaagctca agatcctcga
     1501 tacgatcatc gagcgcctgc gaagcgccaa gaaccagccc agggtcgaac gcgaggccac
     1561 gggcctatcc gacgaacgca tcgacgcgct gatcgagcag tatggggctc agctccacaa
     1621 cgccctcggc gcgggaccgt acgccaagcc gaccggggtg gtcccgggta tgctcgtctc
     1681 catcgccgcc tagttcctca tctccgcctg agtggcacga agcttattgg tatctcgatg
     1741 cggtagatga aacctgcccg aacgccgagg cccgatcgct acaactcggc cgcggcgagc
     1801 cggacaagcg ctatacgtac ataacccaca agattcgtaa cactaaagct aggcctaggg
     1861 gcgcggaccc tcgccgagta gccacgccat ataggcgcgc aggtcgacct ctccatagag
     1921 cgcggcgtaa gacaacgcgg cgtgaccgac gttgcggccg tattgcctgg tctccgcgta
     1981 cgacagcgcc tcgagcagca ggtcgagcgg tagcgccccg tactcggctt cccagcgcct
     2041 gaagcgcgtc gggccgccgt tgtacgagaa tacggccggg aggaccctac cggccagccg
     2101 atcgaggagc cgggctaaat aggacgagcc tatcgtcacg ttatcgagcg ggtcggtcag
     2161 ctcgtagtcg tccaggtcca ggcgctcggc ggtctcagcg gcggtcgccg gcatcagctg
     2221 gatgagcccg acggccccgg acttggatac ggccttcggg tcgaaggcgc tctcggagcg
     2281 cgcgagcccg tagagcaggt gggggtcgag gccggtcgcc tcgatcgccg gactgaaggc
     2341 ctcggggtag ggccgcggcc agtacagctc ggcgtcgctg cgagtcggct cgaagccggt
     2401 tctccagaac agagtcgcta taagtcggta cgcctgatcg tgccgtccgg cggcgtcgag
     2461 ctcggcggct atcgtcctga ccgcgttctc ggacacggat cggaactcgt ccccgagctc
     2521 ggcccggacg cggtaaccga gcccgaagcg agtcagggct agggcgtact cgtccgggct
     2581 gacggggcgg cgcgcctcgt cgtccgcaga tgccgtcccg ccactctcgg tgggggccgg
     2641 ctcggttccg gggacggcga gcggctcgac gaggggctcg ccgagtctgt aggcggcgac
     2701 gagtcggtac catgggtcga cccgctggcc gtacgcctcg tgtagcctcg agtcgatgta
     2761 accgccaagg tcgacgaagg agccg
//
LOCUS       DAOOLN010000114         2759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_44986_length_2759_cov_2.852811, whole genome shotgun sequence.
ACCESSION   DAOOLN010000114 DAOOLN010000000
VERSION     DAOOLN010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44986_length_2759_cov_2.852811"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1494)
                     /locus_tag="PLW80_02165"
     CDS             complement(<1..1494)
                     /locus_tag="PLW80_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB65334.1"
                     /translation="MAKTSYATLFDTALEAGARRDYAEAETTLTRIVAETDAMPEAWL
                     YLGRARYALGDYGRAIPAFSTFLGYDPESAAGWFFLGRGYLAIGRNRAAAYSLQRAID
                     KGRAGADAWALLGFAELRQKRSAKAVACLERAISLAPNDHRVYRAYLNALYVHAIRSL
                     ARGQAAEAASMLGFVIDNGLDGPAQRLYRSRALRASGDVPSAVAELELAVRAEPDDPS
                     LRLQLAALRFSAGDPDGAMAEIERSGVSLPEQRGAPWTADAVERWRVIVALRDGDYRT
                     ALKAALERIRRGDADAAIRAVAAQANMELGRYDRAAAHYQRAVEADPGAPDLRIGLAL
                     AKYELGDYQAARAAARSATARGAARDDSLYVELLCDVKTGVDPRASLPTAQALLRARP
                     GDPRLMMALAECLYKTGEAALADGWFADVLRLWPSHELAMLYRVSVAESLGNDDDALE
                     RYGEYLASFPDNAAVRRDYIDALVASESWDRAATAIEEGKAYGAIHGS"
     gene            1493..>2759
                     /locus_tag="PLW80_02170"
     CDS             1493..>2759
                     /locus_tag="PLW80_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPB65335.1"
                     /translation="MRILYRSGGQSYRGGRCLEGRGGVQCGMRDQTRKRLGRLAFPLG
                     AGVLMLALAASLWALTLRERANRRLYLEYEVHRVVTAMTDLVRLRTLEAGDVQDVVGF
                     GLYAADGSAVSLYGEAPASMNVTTMGYAPSSFTLRESSVVMIRSIGGDLLGRRNMMGP
                     ERMATDRAARWRDIMQGRQGSAGALPPPAEPPESPQAGSFARTGRQPAISYIEFSTQG
                     FLAEQRLFLVIAVAATAAIAGLYLMLLAMNRGYVEAKDREVRDRELVELGQAARTIAH
                     EIKNPLGVIRIQCGLLRRGAPEGIVAGLAIIDDEALRLAALADRIRSFLKSGEAKPER
                     LDALGYLTDFGARYNGALELAVDVGAGTSVLVDAARLTEALDNLVSNAIDASRGAAEP
                     PRLEAMVRSRRLAIAVLDRGGGVPADRADR"
BASE COUNT          437 a          916 c          979 g          427 t
ORIGIN      
        1 cgagccgtgt atggcgccat aggccttgcc ctcctcgatg gcggtggcgg cccggtccca
       61 ggattccgac gcgacgagcg catcaatgta gtccctgcga acagccgcgt tgtccgggaa
      121 cgaggcgagg tactcaccat agcgctccag tgcgtcgtca tcgttgccta aggactcggc
      181 gacggacacg cggtacagca tggccagctc gtgcgatggc cagagccgta gcacgtcggc
      241 gaaccagcca tcggccaggg ctgcctcgcc cgtcttgtac aggcactcgg ccagcgccat
      301 catgagcctc gggtctccgg gacgggcgcg taacagggct tgcgccgtcg gtagcgaggc
      361 gcggggatcc acgccggtct ttacgtcgca gagcaattcg acgtagaggc tgtcgtcgcg
      421 ggcggccccg cgcgccgtcg cgctcctcgc ggcggccctc gcggcttggt aatcgcccag
      481 ctcgtacttg gccagagcca gcccgatccg gaggtcgggc gcgccggggt cggcctcgac
      541 ggcgcgttgg taatgggccg cggcgcggtc atagcggcca agttccatgt tcgcctgcgc
      601 ggcgacggct cggatggccg cgtccgcgtc gccgcgcctg atacgctcga gcgccgcctt
      661 cagtgcggtc ctgtagtcgc cgtcgcgtag cgcgacgatc acccgccagc gctcgacagc
      721 gtcggccgtc cacggcgcgc cacgctgctc ggggagggaa acacccgagc gctcgatctc
      781 ggccatcgcg ccatccgggt ctcccgccga gaaacgcagg gccgcgagct gcagcctgag
      841 cgacgggtcg tcgggctcgg cgcgcaccgc cagctcgagc tcggcgacgg cagacggcac
      901 gtcgccggag gccctgagcg ccctcgacct gtagagtcgt tgagccggcc cgtcgagccc
      961 attgtcgatc acgaagccca gcatcgaggc ggcttcggcg gcctggcctc gagccagcga
     1021 gcggatggcg tgcacgtaga gcgcgttgag gtaggctcgg tagacgcgat ggtcgttcgg
     1081 cgccaggctg atagccctct cgaggcaggc gacggccttg gccgagcgtt tctgtcgcag
     1141 ttcggcgaaa ccgagcagcg cccaggcgtc ggctccggcg cgccccttgt cgatggcacg
     1201 ttggaggcta taggcagcgg cgcggttacg gccaatggcc aggtacccgc gtcccaggaa
     1261 gaaccaaccg gcggcgcttt cgggatcgta gccgaggaag gtagagaagg cgggaatggc
     1321 gcgcccgtag tcgccgagcg cgtacctggc cctgcccagg tagagccagg cctctggcat
     1381 ggcgtcagtc tcggcgacta tcctcgtcag cgtagtctcc gcctcggcgt agtccctgcg
     1441 ggcgccggct tcaagggccg tatcgaatag ggtcgcgtag gaagtcttgg ccatgaggat
     1501 actgtacagg agcggcggcc aatcgtacag gggagggcgt tgcctcgagg gccggggcgg
     1561 cgtacaatgc ggtatgcgcg atcaaaccag gaagcgcctc gggcggctgg cctttccgct
     1621 cggcgccggc gtgctgatgc tggcgctcgc ggcgtcgctc tgggcgctca cgctcaggga
     1681 acgggccaat cgtcgcctgt acctcgagta cgaggtccac agggtcgtca ccgcgatgac
     1741 cgacctcgtc aggctcagga cactcgaggc cggcgacgtc caggacgtgg tcggcttcgg
     1801 tctatacgcc gcggacggta gcgccgtctc gctctacggt gaggcccccg cgtcgatgaa
     1861 cgtcacgacc atgggctacg ccccttcgag cttcactctg cgggagtcgt cggtcgtcat
     1921 gatacgatcg atcggcggag atctgttggg ccggaggaat atgatgggtc ccgagcgaat
     1981 ggcaacggat cgcgcggccc gatggagaga tatcatgcag gggcggcaag gctcggccgg
     2041 cgcgctaccg ccgccggccg agccgcccga aagcccccag gccgggtcct tcgcgcggac
     2101 tggccgccag ccagcgatat cctacatcga attctccacg cagggattcc tagccgagca
     2161 gcggctattc ctagtgatcg cggtcgccgc gaccgcggcc atcgcgggac tatacctgat
     2221 gcttctagcc atgaaccggg gctacgtcga ggccaaggac agggaggtac gcgatcgcga
     2281 gctcgtcgaa ctcgggcagg ccgccaggac tatcgcccac gagatcaaga acccgctcgg
     2341 tgtcatacgc atccagtgcg gcctcttgag gcgcggagcc cccgagggga tcgtagccgg
     2401 tctggccatc atcgacgacg aggccttacg gttggccgcg ttagccgacc ggatacgaag
     2461 cttcctcaag tcgggcgaag cgaagcccga gaggctcgac gcgctcggct atctgaccga
     2521 cttcggggcc cgctataacg gcgcactcga gctggccgtc gacgtcggcg ccgggacctc
     2581 ggtcctggtg gacgcggcca ggctgaccga ggccctggac aacctggtgt cgaacgccat
     2641 cgacgccagc cgaggggccg cggaaccgcc tcggctcgag gccatggtca ggtcgcgacg
     2701 cctggcgata gcggtactcg accgtggcgg cggcgtgccg gcggatcggg ccgatagga
//
LOCUS       DAOOLN010000115         2748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_45214_length_2748_cov_3.734497, whole genome shotgun sequence.
ACCESSION   DAOOLN010000115 DAOOLN010000000
VERSION     DAOOLN010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45214_length_2748_cov_3.734497"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PLW80_02175"
     CDS             <1..300
                     /locus_tag="PLW80_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65336.1"
                     /translation="LMITKPSELSVYPVPKLFIQHVGRHEVWGAIRGSELGDGTLETE
                     SDASLRQALRLLVEEDDLLEMYCGNILKNYRAGVYDGAYGAVHLALERRSDGAGR"
     gene            424..948
                     /locus_tag="PLW80_02180"
     CDS             424..948
                     /locus_tag="PLW80_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HPB65337.1"
                     /translation="MDKYRFTYQAIHRTVRDLADRVLAAGYETDIIVAIGSGGFIPAR
                     MLRTFINKPILAVGIAYYDQDNQPTDNPRKVQWIEEAERQLKGKRILLVDEVDDSRVT
                     LEYCVRELLSHHPESVAVAVLHNKLKEKRGAMPPEVKLYLSGLDIEDKWVCYPWDAQD
                     IDRHDRDAATTGQA"
     gene            945..1583
                     /locus_tag="PLW80_02185"
     CDS             945..1583
                     /locus_tag="PLW80_02185"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HPB65338.1"
                     /translation="MIAPDEGRRDVAALKAALRKSAKAALAAVTPERRAVASADAAAR
                     ASAHPAWPGAELVLAFLSMPSEIDTAPVVEAALAAGKRVAVPRIDGGDIAFVELAPDW
                     RAWPRDRWDIPAPPAEAPSIPPAEIAGLATLALVPGLAFDRTGGRLGRGKGYYDRFLA
                     SVAAARSAAAARSSGPSSPFVAIGYGYESQIVDSVPTDERDLPLDGLSLGQA"
     gene            1635..2111
                     /locus_tag="PLW80_02190"
     CDS             1635..2111
                     /locus_tag="PLW80_02190"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HPB65339.1"
                     /translation="MQAKKRVALVAHDNRKLDLVGWVEYNWRSLLKHELVCTGTTGRL
                     VSETIERKRSTEGGAGAPASSITLLKSGPLGGDQQLGAMIAEGRIDAVIFFWDPMSPQ
                     PHDVDVKALLRIAVVYNIPMACNRSTADFMISSPLFERAYDPIVKDYSAYIQRKVD"
     gene            complement(2108..>2748)
                     /locus_tag="PLW80_02195"
     CDS             complement(2108..>2748)
                     /locus_tag="PLW80_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65340.1"
                     /translation="SQAVSARVALGWEFSNVVFMGMGEPLDNFDGLAGALAVMFDQSA
                     LGLAQERVTVCTVGLPAGIARLKGLGYRRLGLSVSLNAPDDGLRSTLMPVGGAHGLGE
                     LAAALAAYPSRRNFALGVNYCLIPGVNDGPGAADGVAAFVRAAGRALVNVIPYNPGTA
                     PIGRAPTEPEVDAFMASLTERGLEARVRAPRGRGLAAACGQLGGPNATVGVP"
BASE COUNT          467 a          975 c          911 g          395 t
ORIGIN      
        1 ctgatgatca ccaagccgag cgagctgtcg gtctacccgg tgcccaagct gttcatccaa
       61 cacgtcggcc gtcacgaggt ctggggcgcc atacgcggct cggagctcgg cgacggtacg
      121 ctcgagaccg agtcggacgc cagcctcagg caggcgctca ggctccttgt cgaggaggac
      181 gacctcctgg agatgtactg cggcaacata cttaaaaact accgcgccgg cgtctacgac
      241 ggggcctacg gcgcggtgca cctcgcgctg gaacggcgat cggacggggc tgggcggtag
      301 cggcacagca tggcgcggcc gaggcggccg aggcttctag ctccagcagt tccttggtgg
      361 agctcgatac gccctcgtca tgactatctt gcgcccgacc gcttgacggg cgtatgatcg
      421 gacatggaca agtaccgatt cacctaccag gccatccacc gcacggtacg cgacctagcg
      481 gatcgcgtcc tggcggctgg atacgagacg gacatcatcg tcgccatagg ctcgggcggc
      541 ttcatcccgg cccgcatgtt gcgtaccttc atcaataagc ccatcctcgc cgtcggcatc
      601 gcctactacg accaggataa ccagccgacg gacaacccgc gcaaggtgca gtggatcgag
      661 gaagccgagc gccagctcaa gggtaagcgc atcctgctcg tcgacgaggt cgacgactcc
      721 cgcgtcaccc tcgagtactg cgtgcgcgag ctcctatccc atcatcccga gtcggtggcc
      781 gtggcagtac tgcacaacaa gctcaaggag aagcgcggcg ccatgccgcc cgaggtcaag
      841 ctctacctat ccggcctcga catcgaggat aagtgggtct gctatccctg ggacgcccag
      901 gacatagacc ggcacgaccg cgacgcggcg actaccggac aggcatgatc gccccggacg
      961 aggggcgacg cgacgtcgcc gcgctcaagg ccgcgctccg aaagtcggcc aaggccgccc
     1021 tcgcggccgt gacgcccgaa cggcgagcgg tagcgagcgc cgacgcggcc gcccgggcta
     1081 gcgcccaccc cgcgtggccg ggagcggagc tcgtgctcgc cttcctatcg atgccgtcgg
     1141 agatcgatac cgccccggtc gtcgaggccg cgctcgccgc gggcaagcgg gtagccgtgc
     1201 cgcgcatcga cggcggcgac attgccttcg tcgaactcgc gccggactgg cgggcctggc
     1261 cgcgcgaccg ctgggacatc cccgcgccgc cggccgaagc tccgtccata ccgccggccg
     1321 agatcgccgg gctggcgacg ctggcgctcg tgccagggct ggccttcgac cggaccggcg
     1381 gacgcctggg gcgaggcaag ggctactatg accgcttcct ggcttctgtg gcagcggcgc
     1441 ggtctgcggc cgcggcgcgg tcgtccggcc cctcgtcgcc cttcgtcgcg ataggctacg
     1501 gctacgagtc ccaaatcgtc gattcggtgc cgaccgacga acgcgacctg ccgctcgacg
     1561 gactatcgct cggccaggcg taggcccgga gccggccgtt ccatcgttct agcgaacctg
     1621 aagaggagat atcgatgcaa gccaagaaac gcgtggcgct ggtggcccac gacaaccgca
     1681 agctggatct ggtcggctgg gtcgagtaca actggcgatc gttgctcaag cacgaactcg
     1741 tctgtaccgg cacgaccgga cgcctggtat cggagacgat agagcgcaag cgctcgaccg
     1801 agggcggcgc cggggctccg gcgtcgagca tcaccctgct caaatccggc ccgctcggcg
     1861 gcgaccagca gctcggcgcg atgatagcgg agggccggat cgacgcggtg atcttcttct
     1921 gggacccgat gtcgccccag ccgcacgacg tggacgtcaa ggcgctcctg cgcatcgcgg
     1981 tggtctacaa catcccgatg gcctgcaacc gctccaccgc cgacttcatg atctccagcc
     2041 cgttgttcga gcgggcctac gatcctatcg tcaaggacta tagcgcctat atacagagga
     2101 aggtcgacta gggaacgccc acggtcgcgt tcgggccgcc gagctggccg caggcggccg
     2161 ccaggccgcg accccgcggc gcccgaacgc gcgcctccag gccgcgttca gtcagcgagg
     2221 ccatgaacgc gtccacctcc ggctccgtgg gggcccggcc tatcggcgcc gttcccggat
     2281 tgtacggtat cacgttgacg agcgcccgcc ccgcggcccg gacgaaggcc gcgacgccgt
     2341 cggccgcccc gggcccgtcg ttgacgcccg gaatcaggca gtagttgacc ccgagcgcga
     2401 agttgcgcct ggacggatag gcggccaggg ccgcggcgag ctcaccgagg ccgtgagccc
     2461 cgccgaccgg catcagcgtc gatcgcaacc catcgtccgg cgcgttcagg ctgaccgata
     2521 ggccgagcct tcggtacccg aggcccttga gccgagcgat gccggccggc aggcccacgg
     2581 tgcagacggt cacgcgctcc tgggccaggc cgagggccga ctggtcgaac atcacggcaa
     2641 gcgccccggc cagcccgtcg aagttgtcca gcggctcgcc catgcccatg aacacgacgt
     2701 tcgagaattc ccagccgagc gcgaccctgg cgctcaccgc ctgggaca
//
LOCUS       DAOOLN010000116         2738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_45449_length_2738_cov_2.598956, whole genome shotgun sequence.
ACCESSION   DAOOLN010000116 DAOOLN010000000
VERSION     DAOOLN010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45449_length_2738_cov_2.598956"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PLW80_02200"
     CDS             complement(<1..315)
                     /locus_tag="PLW80_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65341.1"
                     /translation="MGIRLVYGLKTDDASYLDSFYPARRLREAAAANGLDYGATVLPP
                     WSTIDDALAFCDGHAALVRGDAPQALYEALESICPFVINGAAATWLAGDKAASAERFA
                     AIG"
     gene            complement(349..2565)
                     /locus_tag="PLW80_02205"
     CDS             complement(349..2565)
                     /locus_tag="PLW80_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB65342.1"
                     /translation="MSEDLFSGSVHTDGRGPLADRMRPRVLDEFIGQDHIVGPGRLLR
                     RAIQRDRLSSIILSGPPGTGKTTLARVIANTTSSRFISINAVLAGVQAIRDAVESAKR
                     ERELYDRKTILFVDEVHRWNKSQQDALLPYVENGTIVLIGATTENPFFEVNKALVSRS
                     RVFQLHPLGDDDLVAAARYALSDRERGYGRWKVEFDDGALEHLVKVAEGDARSLLNAL
                     ELAVETSVEPWPPADGERVLVTLDAAEQSIQRRAVLYDRDGDYHYDASSALIKSLRGS
                     DPDAALYWLARMVYAGEDPRFIFRRLLISAAEDVGLADPAALGVVASCAEAYERIGLP
                     EGQFLLAEAALYLATAPKSNSALAYFDALAAVEAERAEVPDHLKDANRDAVGLGHGEG
                     YLYPHAYRSHWTAQQYLPDSLKGAVFYQPGSLGHEARVRDEVLRRRDAQLAAWLSGEL
                     DVPRPTAAPTSHAQAASWTARADSSSAGGLSGLRDLVFKLAFPPRHANVLVLAADDGL
                     MLREAVRTCVEGSACGVCRDAASIDRLAYAFKDVEELLRPSLAACADAARVADTAMEA
                     AGHAVFDLVVAAGFLSSYGAEAAVEGGRALVERLRADGGRRAFVFCERSPVAGALPTI
                     VAPLLEAGTLAAFTAADEAFFASSAAPGFSRERLEEAIVAMGLEPRRSEPYRYDTRRS
                     VGERDVLAWLSADSAYGSAIASAIGAEVVAVVLDALKKGIESGQAAWPNLWNVTLA"
BASE COUNT          421 a          901 c         1013 g          403 t
ORIGIN      
        1 ccctatcgcg gcgaagcgtt cggccgaagc ggccttatcg ccggcgagcc aggtcgcggc
       61 cgcgccgttg atgacgaagg ggcagatcga ttcgagcgcc tcgtacaggg cttgaggcgc
      121 gtcgccgcgg accagagccg cgtggccgtc gcagaaggcc agggcgtcgt cgatcgtcga
      181 ccaaggcggc aggaccgtcg cgccatagtc cagaccgttg gcggcggcgg cctcgcggag
      241 gcgcctggcg gggtagaagg agtcgaggta tgatgcgtcg tcggtcttga gtccgtagac
      301 gagcctgatg cccatggagg cggagtatag cgacctatcg cggccggctc aagccagcgt
      361 cacgttccag aggttcggcc aggcggcctg gcctgattcg atgcctttct tgagggcgtc
      421 gagtacgacg gcgacgactt cggcgccgat ggccgaggct atggccgacc cgtaggccga
      481 gtcggccgat agccaggcca ggacatcgcg ctcgccgacg gagcgccgcg tgtcgtagcg
      541 gtacggctcg ctccgtctcg gttcaaggcc catggcgacg atggcctcct cgaggcgctc
      601 ccgggagaag cctggcgcgg cgctcgacgc gaagaaggct tcgtcggcgg ccgtgaacgc
      661 cgcgagcgtt cccgcctcga gcagcggggc gacgatggtc ggcaaggcgc cggctaccgg
      721 cgagcgctcg cagaacacga aggcccggcg cccgccgtcg gcgcgtagcc gctcgaccag
      781 ggcccgcccg ccctccaccg cggcctcggc tccgtaggag gacaggaacc ccgcggcgac
      841 gaccaggtcg aagacggcgt ggcccgccgc ctccatggcc gtgtcggcca cgcgcgcggc
      901 gtcggcgcag gcggcgaggc tcgggcgaag aagctcttcg acgtccttga acgcgtaggc
      961 caggcggtcg atcgaagcgg cgtcgcggca gacgccgcag gcagagccct cgacgcaggt
     1021 cctgaccgcc tcgcgaagca tgagtccgtc gtccgccgcg agcacgagca cgttcgcgtg
     1081 cctcggcggg aacgccagct tgaagacgag gtcgcggagg ccggagagcc cgccggccga
     1141 gctcgagtcg gcgcgagccg tccaggaagc cgcctgcgca tggctcgtcg gcgcggccgt
     1201 cggccgcggg acgtcgagct ctccagacag ccaggccgcc aactgggcgt cacggcgcct
     1261 gagaacctca tcgcgcacgc gagcctcgtg gccgagcgag cccggctggt agaagacggc
     1321 gcccttgagc gagtcgggta ggtattgctg ggcggtccag tggctgcggt aggcgtgcgg
     1381 gtagaggtag ccttcgccgt ggccgaggcc cacggcatcg cggttggcgt ccttcaggtg
     1441 atccggcacc tcggctcgct ccgcctcgac ggccgcgagc gcgtcgaagt aggcgagcgc
     1501 cgagttggat ttgggcgcgg tggccaggta cagggcggcc tcggccagga ggaactgacc
     1561 ctcgggcagg ccgatgcgct cgtaggcctc ggcgcaggaa gccaccacgc cgagggcggc
     1621 cgggtcggcc aggccaacgt cctcggccgc cgagatgagg agtcgcctga agatgaaccg
     1681 cggatcctcg ccggcgtaga ccatgcgagc cagccagtac agggccgcgt cggggtcgga
     1741 gccgcgcagg ctcttgatca gggcgctcga ggcgtcgtag tggtagtcgc cgtcgcggtc
     1801 gtacagtacg gcgcgtcgct ggatgctctg ctcggcggcg tcgagcgtga cgaggacccg
     1861 ctccccgtcg gccggtggcc agggctcgac gctcgtctcc acggccagct cgagcgcgtt
     1921 gagaaggcta cgcgcgtcgc cttcggccac cttgaccagg tgctcgaggg cgccatcgtc
     1981 gaactcgacc ttccatcggc cgtagcctcg ctctcggtcg gacagggcgt aacgcgcggc
     2041 ggcgaccagg tcatcgtccc cgagcggatg gagttggaac acgcgcgagc gcgacacgag
     2101 ggctttgttc acctcgaaga agggattctc cgtggtcgca ccgatcagga ctatggtgcc
     2161 gttctcgacg tagggcagga gggcatcctg ctgggacttg ttccagcggt gcacctcgtc
     2221 gacgaagagt atggtcttgc ggtcgtacag ctcgcgctcg cgcttggcgg actcgacggc
     2281 gtcgcgtatg gcctggacgc cggcgagcac cgcgttaatc gaaatgaaac gcgagctggt
     2341 cgtgttggct atgactcggg cgagcgtcgt cttgccggtg cccggaggac cggagaggat
     2401 gatcgacgac aggcggtcgc gctggatcgc ccgcctgagc agacggccgg gaccgacgat
     2461 gtggtcctgg cctatgaact cgtcgaggac gcgcggccgc attctgtcgg ccagcgggcc
     2521 gcgcccgtcc gtatggacgg agcccgagaa taagtcctcg ctcacggccc gctattccct
     2581 gacccagcct ccccaggcgc cgttctcgta ggtgttggac catgggccga caccgtcggt
     2641 cagagcgaat atcctgcgct tgccaccgcc aaggtcgaac gagggcatcg aggtgatcga
     2701 cgtgcctgac aggctcgccg cgaaattcgt ggcgtccg
//
LOCUS       DAOOLN010000117         2733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_45558_length_2733_cov_3.166542, whole genome shotgun sequence.
ACCESSION   DAOOLN010000117 DAOOLN010000000
VERSION     DAOOLN010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45558_length_2733_cov_3.166542"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PLW80_02210"
     CDS             <1..534
                     /locus_tag="PLW80_02210"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB65343.1"
                     /translation="SPRSRPPRDGVAARSLGRSGRFLPALLLLGKASLLSGDDEKAVA
                     ALKRALAATPRAGEAAVWLARAYRASGLAAEASALCAIAVSADPGSVTALRLMAGLAY
                     DDERLADAKAYLDRALEASAEVGMAFIDRASIRWASGDRTGALADLDAAVTVLPVESN
                     AQKAAVSLGQRIREPTP"
     gene            531..1988
                     /locus_tag="PLW80_02215"
     CDS             531..1988
                     /locus_tag="PLW80_02215"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPB65344.1"
                     /translation="MRTSQTLCAPVAAVALTLGACAGYYVDYRYATSVELDALASVRG
                     YPETIIAGEPLGVAEAAAYAIANDQALSSDREALDLREGRWRLAFRSFLPSVTMGASA
                     DETLAAVGAESLSRSMSASVEQALWDGGRLAARRALESVELGLARLELERSTYETGEG
                     AVASYRGVMTARARLEIQTAALDAARLSRAALASELGLGLASADELLEADLEIGDAEI
                     ALSQASLELSEAEGNLAYALGVDKLPPLADGGLGQRPELKLDADELWRHVVELSPELR
                     EARYRIVAKRAEARAAAAGWLPAIGLTASWRLGGDRFPLSSLSWSVGARVEFSGPLAK
                     VGMGLETGGDGRRERNAANTLSAEPVPDPAGIMDIRSARLALRVEEEAYAAALRELRV
                     RTMAATRAYEHSLARRRLCRDAARLASIRFDSVGLRRDLGRATTLEALRAGLERADAE
                     IDLLEACASIVEAEREVERLIGLPPDGLCAFVEGL"
     gene            1985..>2733
                     /locus_tag="PLW80_02220"
     CDS             1985..>2733
                     /locus_tag="PLW80_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HPB65345.1"
                     /translation="MSSGFKRMWNSSAKALVASLALSTASCGASAAAATEEVLPVRVA
                     VATSLTLDDEIQGYGAIAYSRKVDLATAQSGVIASLPYREGEKVEKGDVVAVVTNPRV
                     LLALGIAENGVASASAELALAEARLFSGRLNAESRLLDIERERMELSLARLELREAER
                     KQADQEALYAAGSVAEETVRSGRFSLESAKGGIELIEREIASNLVGLRDEDLVARGLR
                     VPEGEAARAAALADLCTESLVAERDAAKASL"
BASE COUNT          434 a          870 c         1014 g          415 t
ORIGIN      
        1 tctccgcgaa gtcgtccacc gcgcgacggg gtggcggcgc ggtcgctcgg gcgctccggg
       61 cgcttcttac cggccttgct cctgctcgga aaggcgtcat tgctgtcggg ggacgacgag
      121 aaggcggtag cggcgctcaa acgggctcta gcggcgacgc ccagggcggg agaggcggcc
      181 gtatggctag ccagggccta tcgggcgagc ggcttggccg ccgaggcgtc ggcgctctgc
      241 gccatagccg tatcggctga tcccggatcg gttacggccc tcagactcat ggccggcctc
      301 gcatacgatg acgagcggct cgccgatgcc aaggcgtacc tcgaccgcgc gctcgaggcg
      361 tccgcggagg tcggcatggc cttcatcgac cgcgcgtcga tacgctgggc gtccggggac
      421 cgcaccggcg ccctcgccga cctcgacgcg gcggttacgg tacttccggt cgagtcgaac
      481 gcgcagaagg cggccgtcag cctgggacaa cggatacggg agcccacgcc atgaggacgt
      541 cgcagacgct atgcgcgccg gtcgcggccg tcgcgctgac gctcggagcg tgcgccgggt
      601 actatgtcga ttatcgctac gcgacgtcgg ttgagctcga cgccttggct tccgtacgcg
      661 gctatccgga gaccatcatc gcgggcgagc cgctgggcgt cgcggaagcc gcggcttacg
      721 ccatagccaa cgatcaggcg ctctcctcgg atcgtgaggc cctcgatctg cgcgaaggcc
      781 gatggaggct cgcgttccgc tcgttcctcc catcagtcac gatgggcgcg agcgccgacg
      841 agaccctggc cgccgtcggc gccgagtcgc tgtcccgttc catgtcggcc agcgtcgagc
      901 aggcgctatg ggacggcgga cggctagccg cgcgtcgagc tctcgagtcg gtcgagttgg
      961 gcttggccag actcgagttg gagcgatcga cctacgagac cggcgagggc gcggtcgcct
     1021 cgtatcgcgg ggtcatgacg gcgagggcac gcctggagat ccagacagcg gccttggacg
     1081 cggctcgcct gagccgagcc gcgctggcgt ccgagctggg tcttggacta gcctcggccg
     1141 acgagcttct cgaagccgac ctggagatag gcgatgccga gatagcctta tcgcaggcga
     1201 gtctggagtt gagcgaggcg gaagggaacc ttgcgtacgc gctcggcgtc gacaagctac
     1261 ctccgttggc ggacggcggc ctcggccagc ggcccgagct taagttggac gcggacgaac
     1321 tctggcggca cgtcgtcgag ctgtctcccg aactcaggga ggcgcgttac cgaatcgtgg
     1381 cgaaacgagc cgaggccagg gccgccgccg cgggatggct cccggccata ggcctgacgg
     1441 cgtcctggcg actaggcggc gatcggttcc ccctcagctc tctatcgtgg tccgtcgggg
     1501 cgagggtcga attctccgga cccctggcca aggtcgggat gggcttggaa acgggcggcg
     1561 acggaaggcg cgagaggaac gcagccaata cgctatcggc ggagccggtg ccggatcccg
     1621 cgggcatcat ggatatacgg agcgcccgct tggccctgcg cgtcgaggag gaggcctacg
     1681 cggcggcttt acgcgagctg cgagtcagga ccatggctgc gactagagcc tatgaacact
     1741 cgttggccag gcggcggcta tgccgagacg ctgcgcgctt ggcgtcgatc aggttcgact
     1801 cggtgggcct gagacgggac ttgggccggg cgacgacgct cgaggcccta cgcgccgggc
     1861 tagaacgagc tgacgccgag atcgatctgc tcgaggcctg cgcctcgatc gtcgaggcgg
     1921 agcgggaggt cgagcggcta atcggactgc cgcccgatgg cctatgcgct ttcgtggagg
     1981 gactatgagc tccgggttca aacggatgtg gaactcgagc gccaaggcgc tcgtcgcctc
     2041 gctcgccctg tcgacggcct cctgcggggc gagcgcggcc gccgcgaccg aggaagtcct
     2101 gccggttagg gtcgcggtag cgacctccct cacgctcgat gacgagatcc aggggtacgg
     2161 ggccatcgcg tactcgagga aagtcgacct ggctaccgcc cagtccgggg taatcgcatc
     2221 cttaccgtac agggagggcg agaaggtaga gaagggggac gtggtcgccg tcgtaacgaa
     2281 ccctcgcgtt ctactggccc tgggaatagc cgagaacggc gtggcgagcg ccagcgccga
     2341 gctcgccttg gcggaggccc gcctgttcag cggtcggctg aacgccgagt cgcggctact
     2401 cgacatcgag cgagagagaa tggagctgag cctcgccagg ctcgagctac gcgaggcgga
     2461 gcgcaagcag gcggaccagg aagcgctgta tgcggcgggg agcgtcgccg aggagacggt
     2521 acgatccggc cgcttctcgc tagagtcggc taagggcggc atagagctga tcgaacgtga
     2581 gatcgcgtcg aacctcgtcg ggttgcgcga cgaggacctc gtggccaggg gcctccgcgt
     2641 acccgagggg gaagcggctc gggccgccgc cctggccgac ctatgcacgg agagcctggt
     2701 cgccgagcgc gacgcggcca aggcctcgct cga
//
LOCUS       DAOOLN010000118         2730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_45626_length_2730_cov_3.384673, whole genome shotgun sequence.
ACCESSION   DAOOLN010000118 DAOOLN010000000
VERSION     DAOOLN010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45626_length_2730_cov_3.384673"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..1037
                     /gene="rsmH"
                     /locus_tag="PLW80_02225"
     CDS             45..1037
                     /gene="rsmH"
                     /locus_tag="PLW80_02225"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPB65346.1"
                     /translation="MSDQKPSFGHVPVMLNDALERLVPPSPDCLLIDGTLGLAGHSSA
                     FLSRYPGLRLIGVDADAEVQAIARARLEPYGDRVSLVNLFFDEFFSGYAAAVARGEAE
                     RPGLVLFDFGVSMFHFRESGRGFSLQGDERLDMRLSSGAERTAADLVAELDERELLRI
                     ISEYGEEPFAYHITKAIVRERAAARIDSTARLADIVRGAVPAKARYGRIHPATRTFQA
                     LRIAVNDELGRIERVLPAAISCLAEGGLIACISFHSLEDRIVKRTFRSLELRKERAYF
                     KEAGDRPIPVGEAERGLVILTKKPVEPADDEVAANPASRSAKLRVARAVGGARS"
     gene            1149..1439
                     /locus_tag="PLW80_02230"
     CDS             1149..1439
                     /locus_tag="PLW80_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65347.1"
                     /translation="MRKVWLTLLVLSIPCMLILTAAQGARYYAVTVELRRLEAAQADW
                     VEENRRLLADIAVAKSRARVGASMAEAEGYCMVGPKTTLRIRVKPGAEKRDG"
     gene            1432..>2730
                     /gene="murF"
                     /locus_tag="PLW80_02235"
     CDS             1432..>2730
                     /gene="murF"
                     /locus_tag="PLW80_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPB65348.1"
                     /translation="MAEDRGACDAAWFAGAAGGYIASGSNASIGRVETDSRKAGPFAL
                     FVAIRGERSDGHDYARRAADAGSPAALVSASWWAAYGPAAFAGSPCAVIVVPDTLAAL
                     QKAAASYRTRFPSLLRFGVTGSAGKTSAKELLAAIVGASRPTVKNPGNLNSDIGLPAS
                     MFLVRPEHEAAVFEMGINRPGEMDELAAVYEPDCALITNVGTAHVGVLGGTRRAIAEQ
                     KKRIASRFDGRQTLIVWEDDDFRDTLMSGVRGRVETYGPRSTDGFEGARDLGLEGWAL
                     RYRGVEARLRLPGAHNLLNALAAIRAGTLYGAGPEHVAAGLEAVSPLSGRTSVIRGEY
                     TVVDDSYNANAESAIAAIGFCDGANVTGGRIYVLGSMKELGAETVPSHRRVGEAVAGS
                     KADAVFFYGDEARDAYEAALAARPDGEYRWFGDYGELESAV"
BASE COUNT          433 a          921 c          944 g          432 t
ORIGIN      
        1 gccgaattcg ccgccgcctc cgagaccctg gacatacaga acctatgagc gaccagaagc
       61 cgtccttcgg acacgtgcca gtcatgctga acgacgccct cgagcgcctc gtcccgccgt
      121 cgccggattg cctcctgata gacggcaccc tcggcctggc cggccactcg agcgccttcc
      181 tgtcgcgcta cccggggcta cgcctgatag gcgtcgacgc cgacgccgag gtccaggcca
      241 tagccagggc caggctcgag ccctacggcg atcgggttag cttggtcaac ttgtttttcg
      301 acgagttctt ctccgggtac gccgccgccg tagctcgggg cgaggccgag cggccaggcc
      361 tcgtgctgtt cgacttcggc gtctcgatgt tccacttcag ggagtccggg cgcggcttca
      421 gcctccaggg cgacgagagg ctcgatatga ggctatcgtc cggcgccgaa cggaccgccg
      481 ccgacctcgt cgcggagctg gacgagcggg aactcctgcg gatcatctcc gagtacggcg
      541 aggaaccctt cgcctaccat attacgaagg ccattgtcag ggagcgcgcc gccgccagga
      601 tagactcgac ggcgcgcctc gccgacatag tacgcggtgc tgtgccggcc aaggcccgct
      661 acgggcgcat ccatccggcg accaggacct ttcaggctct acgcatcgcg gtcaatgacg
      721 agctcggccg tatcgagcgg gtcctgccgg ccgccatctc ctgcctcgcc gagggcgggc
      781 tcatcgcttg catcagcttt cactcgctcg aggaccgcat cgtcaagcgc accttccgct
      841 cgctggagct acgcaaggaa cgggcctact tcaaagaggc gggcgatcgg ccgatacctg
      901 taggagaggc ggagcgcggc ctcgtcatcc ttaccaagaa gccggtggaa ccggctgatg
      961 acgaggttgc ggccaatccc gcgtccagga gcgccaagct ccgggtcgcg agagccgtag
     1021 gaggggcgcg atcatgatag gaacgatcgg catggccgag ttcatcatgg aagcggtggc
     1081 cgtagtggcg ctgagcgtcg cggccatagc ctgcatccct cccgtgcccc gccagggaag
     1141 gtcgtacggt gaggaaagtc tggctgacgc tcttagtcct gtctattccg tgcatgctga
     1201 tactgacggc cgcccagggc gcgaggtact acgccgtgac ggtcgagttg cggcgcctag
     1261 aggcggcgca ggcggattgg gtagaggaaa acagaaggtt gctcgctgat atagccgtgg
     1321 ccaagtcgcg cgccagggtg ggcgcgtcga tggccgaggc cgagggctac tgtatggtcg
     1381 gccctaagac gacgttgagg atacgggtta agcccggggc ggagaaacgg gatggctgag
     1441 gataggggag cctgcgacgc ggcttggttc gcgggcgcgg ccggcggata catcgcgagc
     1501 ggatcgaacg cgtccatagg gcgcgtcgag accgattccc gcaaggccgg tccgttcgcg
     1561 ctcttcgtcg ctatacgcgg tgagcggagc gacggccacg actacgcccg ccgggcggcg
     1621 gacgcgggct cgccagccgc gctcgtctcg gcttcctggt gggccgccta cggcccggcc
     1681 gcgttcgcgg gttctccctg cgcggtcatc gtcgtcccgg acacgctcgc cgccctgcag
     1741 aaggcggccg cctcctaccg gacacgcttc ccctcgctcc tgcgtttcgg cgtcaccggc
     1801 tcggccggca agaccagcgc caaggagctg ctcgccgcga tagtcggcgc gtctcgcccc
     1861 accgtcaaga acccgggcaa cctaaattcc gacataggcc ttccggcatc gatgttcctc
     1921 gtccggccgg agcacgaggc cgcggtgttc gagatgggca tcaaccggcc cggcgagatg
     1981 gacgagctgg cggcggtgta cgagccggac tgcgccctca tcacgaacgt cggcacggcc
     2041 cacgtcggcg tgctcggcgg tacccgaagg gccatcgccg agcagaagaa gcgcatagcc
     2101 tcccgcttcg acggcaggca gaccctgata gtctgggagg acgacgattt ccgcgacacc
     2161 ctcatgtccg gcgtccgggg ccgcgtcgag acctacgggc cccgctcgac cgacggcttc
     2221 gagggcgcgc gcgatctggg gctcgagggc tgggcgctac gctaccgcgg cgtcgaggcc
     2281 aggctcaggc tacctggcgc ccataacctg ctcaatgcgc tcgcggcgat ccgcgccggg
     2341 acgctgtacg gcgccggacc cgagcacgtc gccgcggggt tggaggcggt ctcgccgctg
     2401 tccggccgga cgagcgtgat ccgcggcgag tacactgtcg tcgacgactc ctataacgcg
     2461 aacgccgaga gcgccatcgc ggccatcggc ttctgcgatg gggcgaacgt tactggcggg
     2521 cggatctacg tgctcggctc catgaaggag ctcggagccg agaccgtccc gtcgcaccgc
     2581 cgcgtcggcg aggcggtggc cggctccaag gcggacgccg tcttcttcta cggcgacgag
     2641 gctcgcgacg cctacgaggc cgccctggcg gcccgtccgg acggtgagta ccgctggttc
     2701 ggcgactatg gcgagctgga gtcggccgtc
//
LOCUS       DAOOLN010000119         2728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_45665_length_2728_cov_3.144033, whole genome shotgun sequence.
ACCESSION   DAOOLN010000119 DAOOLN010000000
VERSION     DAOOLN010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45665_length_2728_cov_3.144033"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PLW80_02240"
     CDS             complement(<1..239)
                     /locus_tag="PLW80_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HPB65349.1"
                     /translation="MGLRGELFSTRMACDGRTYFFNVKENRMGDLFLSIVESKPTETG
                     DFDRRSIVVFQDNADEFLKVGSRADSRDVLEGGLSR"
     gene            complement(291..2252)
                     /locus_tag="PLW80_02245"
     CDS             complement(291..2252)
                     /locus_tag="PLW80_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65350.1"
                     /translation="MEPTTIIHPATGFAIGDPYTDPLATRPTPEKARAFFDTLILSAS
                     GWRGVFGSSDDDLGEAITPAAAYAVARMADTFAGFLMRGRVGVGAPVRAPVVAPVRAP
                     AVALGVDSRPTGPAIADVMARVFLTRGLSVRYAFVCAAPEIMAWVRRAGALPADSPER
                     LDAFCYVSASHNPPGHNGVKFGLSDGGVLPGPEAKALIASLRDGACTAADIAAMAALS
                     AAAEPRAVAALYSGCATAKRRAISDYTLFAREVAGASPELAEQERELDALEAAIAARG
                     AGVVAEMNGSARSLSIDGDYLASIGVATLRLNDEPRRFAHRIVPEGESLEPCRLALAE
                     ARRADPRFSLGYVPDCDGDRGNIVYWDEAEGEARALEAQDTFALAALAELSRSAARAF
                     AGNEAPRLAVACNDATSLRVEALASALGAEAFRAETGEANVVGLARSLRERGYEVRVL
                     GEGSNGGVITHPASVRDPLNTMTALLKLLYLRDDGDAPGPFRLWLERSGQGEAYREDF
                     GLADVLASLPRFSTTSVFEDRAALRVRSRDHAALKNAYESIFRRRWPTIAKELEPRLG
                     PLSWKALASSGQSERELPGRFGDSGSGGLKLLLSNAAGQPAGFLWMRGSGTEPVFRVM
                     ADVRGADATLEGLLLRHHTEMVTEADSIA"
BASE COUNT          437 a          905 c          959 g          427 t
ORIGIN      
        1 cgcgaaagcc cgccctcgag cacgtcacgg ctgtcggcgc ggctgccgac cttgaggaac
       61 tcgtcggcgt tgtcctgaaa cacgacgatg gaccgacgat cgaagtcgcc ggtctcggtg
      121 ggcttgctct cgacgatcga gaggaagagg tcgcccatgc ggttttcctt gacgttaaag
      181 aagtacgtcc tgccgtcgca tgccatgcgc gttgaaaaga gctctccacg aaggcccatc
      241 gattcccctc cctatgaata cacttgcgga tactaacgct ttatgcggtt ctatgcaata
      301 gagtccgctt cggtcaccat ctccgtgtgg tgccggagga ggagcccttc gagcgtcgcg
      361 tcggccccgc gcacgtcggc catcacgcgg aacaccggct ccgtgcccga gccgcgcatc
      421 cacaggaacc cggccggctg gccggcggcg ttcgacagga gcagcttgag gccgcccgag
      481 cccgagtcgc cgaagcggcc cggcagctcg cgttccgact ggccggagct ggcgagcgcc
      541 ttccaggaca gcggcccgag cctcggctcg agttccttgg cgatggtcgg ccagcggcgg
      601 cggaagatgg actcgtaggc gttcttgagg gccgcgtggt cgcgcgatcg cacgcgcagg
      661 gcggccctat cctcgaagac gctcgtcgtc gagaagcgcg gtaggctcgc tagcacgtcg
      721 gccaggccga agtcctcgcg gtaggcctcg ccctgcccgg accgttcgag ccacagcctg
      781 aaggggccgg gcgcgtcgcc gtcgtcgcgt aggtacagca gcttgagcag ggcggtcatc
      841 gtgttgagcg ggtcgcgcac gctcgccgga tgggtgatga cgccgccgtt cgagccctcg
      901 ccgaggacgc ggacctcgta gccgcgctcg cgcagggaac gggccaggcc gacgacgttc
      961 gcctcgccgg tctcggcccg gaacgcctcg gcgccgagtg cggaggccag cgcctcgacg
     1021 cggaggctgg tcgcgtcgtt gcaggcgacc gcgagcctcg gcgcctcgtt cccggcgaag
     1081 gcgcgggcgg ccgagcggga cagctcggcg agcgcggcca gcgcgaaggt gtcctgggcc
     1141 tcgagcgccc tggcctcgcc ctcggcctcg tcccagtaga cgatgttacc gcggtcgccg
     1201 tcgcagtcgg gaacgtagcc gagcgagaag cgcgggtcgg cccgcctcgc ctcggccagc
     1261 gccaggcggc acggctcgag cgactcgccc tccggcacga tgcggtgggc gaagcggcgc
     1321 ggttcgtcgt tcaggcgcag ggtggcgacg cctatcgacg ccaggtaatc gccgtctatc
     1381 gacagcgacc gggccgagcc gttcatctcg gccaccacgc cggcgcccct ggccgctatc
     1441 gcggcctcga gcgcgtccag ttcgcgttcc tgctcggcga gttccggaga ggctccggcc
     1501 acctcgcggg cgaacaaggt gtagtccgag atcgcgcggc gcttggccgt ggcgcagccg
     1561 gagtacagcg cggccaccgc ccgcggctcg gcggcggcgc ttagcgccgc catcgcggct
     1621 atatcggccg ccgtgcaggc gccgtctctg agcgaagcta tcagggcctt ggcctccggg
     1681 ccgggcagca cgccgccgtc ggacaggccg aacttgacgc cgttatggcc gggcgggtta
     1741 tggctcgcgg agacgtagca gaacgcgtcg agccgctcgg ggctgtcagc tggcagggct
     1801 ccggcgcgcc tgacccaggc catgatctcc ggcgcggcgc agacgaaggc gtagcgcacc
     1861 gacaggcctc gggtcaggaa gacgcgggcc atcacgtcgg cgatggccgg cccggtaggc
     1921 ctcgagtcga cgccgagcgc gacggcgggc gcccggacgg gcgcgacgac gggcgcgcgg
     1981 acgggcgccc cgactcccac ccgcccgcgc atgaggaagc cggcgaaggt atcggccatc
     2041 ctggcgacag cgtaggcggc cgcgggcgtg atagcctcgc ccaggtcgtc gtccgacgag
     2101 ccgaacacgc cgcgccagcc ggacgccgac aggatgagcg tatcgaagaa ggcgcgcgcc
     2161 ttttcgggcg tcggcctcgt agcgagagga tcggtatatg ggtcgcctat cgcgaagccc
     2221 gtggcgggat ggatgatcgt tgtaggttcc atgcgcgtat tatagtaaaa aacgatgccg
     2281 aggaaagacc ccgatccgca cgggccgttc taggctccgt cacgcgaagg cgccaaggca
     2341 cggaggacgt ggcatacgcg aagacgagat cttcttaggc aaggcctcac accaaggcac
     2401 taagggatag ggttaagacg gaacaggatg atcaggatgg cgcaggatga ccaagaagaa
     2461 cgctaaggtc ttgtttattt cccttgtaca tcgtgtcttc atcttgtcat cctgttcgta
     2521 ttcgacaatc cgccgccggg ctcagtcgct cgggagccat gttccgtcgc cggggagcca
     2581 tgttccgtcg ccgggaacca tgtttcgtcg ctcgggagcc atgtttcgtc gctcgggaac
     2641 catggttcgt caccgaagaa ccatgtttcg tcgccgagga accatttttc gtcgccggga
     2701 accatgtttc gtcagcggga accatgtt
//
LOCUS       DAOOLN010000120         2723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_45778_length_2723_cov_3.563718, whole genome shotgun sequence.
ACCESSION   DAOOLN010000120 DAOOLN010000000
VERSION     DAOOLN010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45778_length_2723_cov_3.563718"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PLW80_02250"
     CDS             complement(<1..772)
                     /locus_tag="PLW80_02250"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HPB65351.1"
                     /translation="MIAKRLLLALLSVSALALAGAQDAELAKALDAAASGYYLPEVRA
                     AFGTFTYEYSGLPTPFSRWVEDRALLAAAGSSRVRVLNRNAAAAMDPVLGATYADFIR
                     ETGAEALLSGRFFDEGGKARVRFELTELSSGILIGAGDWYVPAQAIPAYASVRPATGV
                     LDRSRELSRLSGSSPGGLSVSVSTDRGAGAAYRVGEALTLLVGVSDDAYARVYHVDGT
                     GRVQLIWPNRFGGGDGHIAAGSPVRLPPDESAPYAFVME"
     gene            904..1716
                     /locus_tag="PLW80_02255"
     CDS             904..1716
                     /locus_tag="PLW80_02255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HPB65352.1"
                     /translation="MPVRLLALDLDDTLLTPELRIHPANLDAVRRALDSGVDVMLASG
                     RTVESMMPYAIELGMKGRGLPMICANGAEVRDVDSGELTRRLTLSADACRAAIEALSG
                     YGLPVQAYDDEGIVVTERNRWTDRDRELTGLPIRVAGSHAEISDKPRSKLISAGEPGR
                     IAELAPVLRARLAGLAEVVVSKPYFIEVLPAGADKGEALAWVAGVRGVPRESVMAIGD
                     AGNDVGMVSWAGVGCAPADARPEVLAVALHVSPLPHDSGAVADLIDRLVLWV"
     gene            1776..1964
                     /locus_tag="PLW80_02260"
     CDS             1776..1964
                     /locus_tag="PLW80_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65353.1"
                     /translation="MSQEMKDLGIKRCYACIQWTGQRTFYPDKKKIKVDEGSDGVCLM
                     THQRIKGSGHCEQYFPLR"
     gene            2046..>2723
                     /locus_tag="PLW80_02265"
     CDS             2046..>2723
                     /locus_tag="PLW80_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPB65354.1"
                     /translation="MTTVRIRILAASILAAAFALEASADIEFVGLDISPDDRILFSAR
                     VEVPGEPGYETLFSADAETGELVQLTFYPESIAVVDDGRRLQIRNRFGVFMTERGFGG
                     LAPLAGIPSFARGSAVQQGRLVDSRPSPDGTMLLYLSPTSPARGDLILFDLSVGKETT
                     VALSIEYSADSFPASWSPDSRYFVYGKGSEIYYFSTEQARAGRVPDESWRRVGAGRVS
                     QARWSANG"
BASE COUNT          437 a          992 c          892 g          402 t
ORIGIN      
        1 gctccatcac gaaggcgtag ggcgccgact cgtccggcgg tagccgcacc ggcgagccgg
       61 ccgctatatg gccgtcgccg cctccgaagc ggttcggcca gatcaactgc accctaccgg
      121 tgccgtccac gtggtagacg cgcgcgtacg cgtcgtcgct gacgcccact agcaacgtca
      181 gcgcctcgcc gacccggtac gcggcgccgg cgccgcgatc ggtgctcacc gacacggaca
      241 ggccgccggg cgaggagccc gacagccgcg atagctcgcg cgacctatcg agcacgcccg
      301 tggccggcct cacggaggcg taggcgggta tcgcctgggc cggcacgtac cagtcgccgg
      361 cgcctatcag gatgccgctc gacagctcgg tcagctcgaa acgaacccgg gccttcccac
      421 cttcgtcgaa gaagcgcccc gacagcagcg cctcagcgcc cgtctcgcgg atgaagtcgg
      481 cgtacgtggc gccgagcacg gggtccatcg cggcggcggc gttgcggttg agtacgcgga
      541 ccctggacga gccggccgcg gcgagcagag cgcggtcctc gacccagcgc gagaacggcg
      601 tgggcaggcc cgaatactcg taggtgaagg tgccgaacgc ggccctgacc tccggtagat
      661 agtatccaga cgccgcggcg tccagggcct tggccagctc ggcgtcctgg gcccccgcca
      721 gggccagggc cgatacggac aacagcgcga gcagcagtct tttagctatc atgcgacctc
      781 catcccgacc acgaacacgc cgtagagtat atcgcgcccg gcgaaacggc gcatcctccc
      841 ggccgcccat ccgtaccggg cccgtccttg tgacgcccgc cgcacccatg gtatacaatc
      901 gacatgccag tacgactact cgccctcgac ctcgacgaca cgctcctgac ccccgaatta
      961 cgcatccacc cggccaacct cgacgcggtg cgacgcgctc tcgactcggg cgttgacgtg
     1021 atgctcgcct cggggcggac cgtcgagtcg atgatgccct acgccatcga gctcggcatg
     1081 aagggccgcg gcctgccgat gatctgcgcc aacggcgccg aggtccgcga cgtcgattcg
     1141 ggcgagctga cgcgcaggct caccctgtcg gccgacgcct gccgggcggc gatcgaggcc
     1201 ctgtccggct acggcctccc ggtccaggcc tacgacgacg agggtatcgt cgttaccgag
     1261 cgcaaccgct ggaccgaccg cgaccgcgag ctgaccggcc tgcccatacg cgtggccggg
     1321 tctcacgccg agatatcgga caagccgagg agcaagctga tctcggccgg cgagccgggc
     1381 cgcatcgccg agctcgcccc ggtcctgcgc gcgcggctcg cgggcctcgc cgaggtggtc
     1441 gtctccaagc cgtacttcat cgaggtgcta ccggccggcg ccgacaaggg cgaggccctg
     1501 gcctgggtcg cgggcgtccg aggcgtgccg cgcgagtcgg tgatggccat cggcgacgcg
     1561 ggcaatgacg tgggcatggt ctcctgggcc ggcgtcggct gcgccccggc ggacgcgagg
     1621 cccgaggtgc tggccgtcgc cctccacgtg tcgcccctgc cccacgactc cggcgccgtc
     1681 gccgacctga tcgaccgtct cgtcctctgg gtttgattgc cttcaactcc ccccgcccgc
     1741 ctgacgagaa taagaacaga cggaaaggag cgagcatgtc ccaggaaatg aaggatctcg
     1801 gtatcaagcg ctgctacgcc tgtatccaat ggaccggcca gcggacgttc tatcccgaca
     1861 agaagaagat caaggtcgac gaggggagcg acggcgtctg cctgatgacc catcagagga
     1921 tcaagggctc cgggcactgc gaacagtact tcccgctgcg ctgatcccgc gataaggcca
     1981 cccagggccg gcttccccta tcgctcgacg gttttttagc cgaaaatgga tcgaagggga
     2041 acgccatgac cactgttcgt atccgcatcc tggccgcctc gatcctagcc gccgctttcg
     2101 ccctcgaggc ctcggccgat attgagttcg tggggctgga catctcgccc gacgaccgga
     2161 tcctgttcag cgcccgcgtc gaggtgccgg gcgagcccgg ctacgagacc ttgttctcgg
     2221 ccgacgccga gacgggcgag ctcgtccagc tgaccttcta ccccgagtcc atagcggtgg
     2281 tcgacgacgg ccgccgcctc cagatacgca accgcttcgg cgtgttcatg accgagcgcg
     2341 gcttcggcgg cctcgcgccg ctggccggca taccgtcatt cgcgcgcggc tccgcggtcc
     2401 agcagggccg cctcgtcgac tcgaggccct cccccgacgg caccatgcta ctctacctgt
     2461 cgccgacgag ccccgcccga ggggacctga tcctgttcga cctctccgtc ggcaaggaga
     2521 cgacggtggc cctgtcgatc gagtactcgg ccgacagctt cccggcctcg tggtcgccgg
     2581 acagccgcta cttcgtctac ggcaaaggct cggagatcta ctacttctcc accgaacagg
     2641 cgcgggccgg gcgcgtaccc gacgagagct ggcgcagggt cggagcgggc cgcgtatcgc
     2701 aggcgcgctg gagcgccaac ggc
//
LOCUS       DAOOLN010000121         2720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_45843_length_2720_cov_3.670544, whole genome shotgun sequence.
ACCESSION   DAOOLN010000121 DAOOLN010000000
VERSION     DAOOLN010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45843_length_2720_cov_3.670544"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="PLW80_02270"
     CDS             complement(<1..1004)
                     /locus_tag="PLW80_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65355.1"
                     /translation="MKRSVSPSMVAGRVVAPPSKSAMQRAVACAAMADGVSTITNPSL
                     CADAIAACRVAEALGASVTRYDDRVVVRGGLSAAPGIVVASCGESGLCLRMFSAIAAL
                     LPVDVELRAEGSLASRSVAMVADALEALGVRCSTNGGLPPVRLRGPLWPGRVRVDGSA
                     SSQFVTGLVMALAMAGGESTVSVERLASGGYVELTFEVMRAFGAEAERDGTEIRIAGT
                     PYRAAEYEVEGDWSGAAFMLVAGAVAGRGDLEVVGLRHGSSQPDRAIVTALEAAGAEL
                     ERVPGGYRVGGGALLGFDFDATDCPDLFPPLVALASRCRGTSRLTGAARLRGKESDRA
                     A"
     gene            complement(988..1518)
                     /locus_tag="PLW80_02275"
     CDS             complement(988..1518)
                     /locus_tag="PLW80_02275"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPB65356.1"
                     /translation="MEKRSYRELGVALVGLPASGKTSSGVLLADLLGMPFVDLDERVE
                     REAGRSIPALFEDEGEDGFRRRERVALEHVAAGVAAGEPVVLATGGGTVELPENRSVL
                     RERFVTVWIKVDPAAAAARSLGSGRPLLSGDVEAGLRRLYERRRAWYDECAAIAVQSD
                     GMRPAMLAEAIREALR"
     gene            complement(1568..2602)
                     /locus_tag="PLW80_02280"
     CDS             complement(1568..2602)
                     /locus_tag="PLW80_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65357.1"
                     /translation="MVHIAHRVAGAVAGLSFLASCVPEPALERFSADDLRPPAVASWG
                     PIGPSEVSIGFDEDIAGAEPGFVSYPGPAVASVALGEDGRSVRVLLDGAQAPGVAYSV
                     SGVVADAAGNLSGFALPYWGHNPDPPAMLINELVTEGSATHPDAVEFYVARGGDCAGL
                     AFFVGSPMDYACRYVFPPLAVAAGDYVVLHLKPQGVAGEVDESTAKDESAGLDASASA
                     WDLWCRGGDGALPGKNGVVALCSSPHGAMLDAVPYSERFADSDTAYGGFGSAALRDRV
                     AAVVAAGAWVGSDPPRPEDCARSAGTTSTRSVCRSSSSGDSDSGADWHVVPTKGASLG
                     SANADEVYAP"
BASE COUNT          428 a         1004 c          957 g          331 t
ORIGIN      
        1 gcggcccggt cgctctcctt gccgcgcagc ctggcggccc cggtcagccg gctcgtccct
       61 cggcagcgcg aggccagggc gacgagcggc gggaacaggt cggggcaatc ggtcgcgtcg
      121 aagtcgaagc cgaggagggc gccgccgccg acccgatagc ccccgggtac gcgctcgagc
      181 tctgccccgg ccgcctcgag cgccgtgacg atggcgcggt caggctggct cgagccatgc
      241 ctcagcccga ccacctcgag gtcgccgcgg ccggcgacgg ctccggcgac cagcatgaag
      301 gcagcgccgc tccagtcccc ctcgacctcg tactcggccg cccggtacgg cgttccggct
      361 atccgtatct cggtcccgtc gcgctcggcc tcggcgccga aggcgcgcat cacctcgaag
      421 gtcagctcga cgtagcctcc gctcgcgagc cgctcgaccg acaccgtcga ctcaccgccg
      481 gccatggcca gggccatcac gagtccggtg acgaactgcg agctcgccga gccgtcgacc
      541 cggacgcggc ccggccagag cggcccgcgc agcctgaccg gcggcagccc gccgttcgtc
      601 gagcagcgca cgccgagggc ctcgagggcg tcggcgacca tcgcgaccga gcgcgaggcc
      661 agggagccct cggcccgcag ctccacgtcg accggcagca gggccgcgat agccgagaac
      721 atgcgcaggc acaggcccga ctcgccgcag gaggcgacga cgattccggg ggcggccgaa
      781 aggccgcccc gcacgacgac ccggtcgtcg tagcgcgtga ccgaggcgcc gagggcctcg
      841 gctacgcggc aggcggcgat cgcgtcggcg cagaggctcg ggttcgttat ggtcgagacc
      901 ccgtcggcca tggcggcgca ggctacggcg cgctgcatgg cgctcttgga cggcggggcg
      961 acgacgcgcc cggccaccat ggacgggcta acggagcgct tcacggatgg cctccgccag
     1021 catggccggg cgcatgccgt ccgactggac ggctatcgcg gcgcattcat cgtaccaggc
     1081 cctcctgcgc tcatagagac gacggagccc agcctcgaca tcgccggaca ggagcggccg
     1141 gcctgaaccg agacttcggg cagcggccgc cgccggatcc accttgatcc agacggtgac
     1201 gaagcgctca cgaaggacgg aacggttctc gggtagctcg acggtgccgc cgccggtggc
     1261 cagcaccacg ggctcgccag cggcgacgcc agcggcaacg tgctcgaggg ccacgcgctc
     1321 ccgccgccgg aagccgtcct ctccctcgtc ctcgaacagg gccgggatcg accgcccggc
     1381 ttcccgttcg acgcgctcgt ccaggtcgac gaagggcatg ccgagcaagt cggccaggag
     1441 gacaccggaa ctggtcttgc cggaggccgg caggcccacg agggcgacgc caagctcccg
     1501 atacgatcgt ttctccatgc cgcatcttat ccggcgcgag tcccgtcggt ctaggccgtg
     1561 ccccggctca aggcgcgtag acctcgtcgg cattggcgga gccgagcgaa gcgcccttgg
     1621 tcggaacgac gtgccagtcg gcgcccgagt cgctgtcgcc cgacgacgat gagcggcaga
     1681 cgctgcgggt gctcgtcgta cccgccgacc tggcgcagtc ctccggcctg ggcggatcgg
     1741 aaccgaccca ggctcctgcc gcgacgaccg ccgcgacccg gtcgcgcagg gccgcagacc
     1801 cgaagccgcc gtaggcggta tcggaatcgg cgaaacgctc gctgtagggc accgcgtcga
     1861 gcatggcgcc atgaggcgac gaacagaggg cgaccacgcc gttcttgccg ggcagggcgc
     1921 cgtcgccgcc gcggcaccag aggtcccagg ctgaagcgct cgcgtcgagg ccagccgact
     1981 cgtccttggc cgtcgactcg tcgacctcgc cggccacgcc ctgaggcttc aggtgcagca
     2041 cgacgtagtc tccggcggcc acggcgagcg gcgggaacac gtagcggcag gcgtagtcca
     2101 tcggcgagcc gacgaagaag gccagaccgg cgcagtcgcc gccccgcgcc acgtagaact
     2161 cgaccgcgtc cgggtgggtc gccgagccct cggtaacgag ctcgttgatc agcatggccg
     2221 gcgggtccgg attgtggccc cagtatggca gcgcgaagcc ggacaggttg cccgccgcgt
     2281 cggcgacgac gccggacacc gagtaggcca cgcccggcgc ctgggcgccg tcgagcagca
     2341 ccctgactga acggccgtct tcgcccaagg cgaccgacgc caccgccggc cctggatacg
     2401 agacgaagcc gggttcggcc ccagcgatgt cctcgtcgaa gccgatcgat acttcggacg
     2461 ggccgatggg cccccaggac gccacggccg gcggccggag gtcgtcggcc gagaaccgtt
     2521 ccagcgccgg ctccggtacg cacgaggcca ggaacgacag cccggcgacg gcgcccgcga
     2581 cgcgatgcgc gatatgaacc ataatgatcc tcccgtcgac catctacgcc gtaaggatcg
     2641 cccgcgcttg catccagtat cgaattgtct gatagtcata gcggtcgatc cttacggcgt
     2701 agatggtcga cgggaggatc
//
LOCUS       DAOOLN010000122         2709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_46098_length_2709_cov_3.100603, whole genome shotgun sequence.
ACCESSION   DAOOLN010000122 DAOOLN010000000
VERSION     DAOOLN010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46098_length_2709_cov_3.100603"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="PLW80_02285"
     CDS             complement(<1..1002)
                     /locus_tag="PLW80_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPB65358.1"
                     /translation="MSLNHALRFKAAIAAVVLVSTLSAACAPSRVGKEDRAPDPAFVV
                     AHSDGIVSRFADLSVVLSSGRDADALRGVNPLSFEPPIAGTASWSADGSRLDFKPAVP
                     LEAGETYRAVFDFASIGEPSNGWFSFRIRAAEPGLSVVPGALYAAFDGSLSLDGSLRS
                     DDAPASADVERLVSATLGGRRLDLSWSHEGDGLHRFTVKRIPRSVRESTLTLSWDGQI
                     IGASAKGSERYRIPAEGSFELLSVTGPEPGESACLRLAFSEPVDKAQDFRGLIRTSSS
                     DTSSPGSLRYESEGGLVRVYSSLGWSEEVGVTVEKGLRGLSGGAIAVPVDSMAPAPEL
                     "
     gene            complement(1080..1730)
                     /locus_tag="PLW80_02290"
     CDS             complement(1080..1730)
                     /locus_tag="PLW80_02290"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPB65359.1"
                     /translation="MKFVFFGAPGAGKGTMAARAAVAFGLPHISTGEIFRAAMRAGTP
                     LGLRVKAVIESGGLVSDELTIGLVRERLGEADAARGWLLDGFPRTLAQAEALAGFCPE
                     DFVIDLEVSDAKVVERLSGRRMCRGCGRSFHVSLMPPAEEGVCDGCGGELYVRDDDGE
                     ASVRSRLEHYYRQTAPLVDFYKRRGTLVSVDGEGRADEVWVALEERMRGLIASAEA"
     gene            complement(1803..>2709)
                     /locus_tag="PLW80_02295"
     CDS             complement(1803..>2709)
                     /locus_tag="PLW80_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65360.1"
                     /translation="SGPMRTDLIARAAARTEEVFDRLGAYSYLSATDRGDFSYYRTFY
                     LEALRRIDDRIADGRLEPVDLSDFPQVIRFPRYEIRAALFIGSFDPFQMTHLATALRF
                     LASDAAGAPVVFVVPEGHDNPSKPRKSDYRYRYELLSMQVAGVFDPLIVPLDIGEGAD
                     TIGIVRRFIAMFPGARVSMTHLLGSDTLPFAASMLPEDLNAWRQESERRRVDFTYEAF
                     VVTREGSPVSASDVEAIRSRGLPIQVDDEPIGTPSSTDFRENRAFSIVFPTESVIRHM
                     EVLFRYNLNKPWSVCGLDEGPGRTR"
BASE COUNT          465 a          913 c          905 g          426 t
ORIGIN      
        1 cagttccggc gccggcgcca tgctgtcgac cggtacggcg atggcgccgc ctgataggcc
       61 tcgtaacccc ttctccacgg tcactccgac ctcctcgctc cagccgagcg acgagtaaac
      121 gcgcacgagg cctccctcgc tctcgtagcg cagcgagcca ggcgacgatg tatcggaaga
      181 cgaggtccga atgagtccgc ggaagtcctg ggccttgtcg accggctcgc tgaaggccag
      241 cctgagacag gcggactcgc ctggttcggg gccggtcacc gataggagct cgaagctacc
      301 ttcggccggt atcctatagc gctccgagcc cttggccgac gcgcctatga tctggccgtc
      361 ccagctgagc gtcagggtgg actcgcgcac cgagcgcggt atgcgcttga ccgtgaagcg
      421 gtggaggccg tcgccctcgt ggctccagga caggtcgagc cggcggccgc ccagggtcgc
      481 cgagacgagc cgctccacat cggccgaggc cggcgcgtcg tcggatcgga gcgagccgtc
      541 gagcgacagg ctgccgtcga acgcggcgta gagggcgccc ggcaccaccg acagcccggg
      601 ctcggcggcc cggatcctga acgagaacca gccattcgac ggctcgccga tggaggcgaa
      661 gtcgaagacg gcgcggtagg tctcgccggc ttcgagcggg accgccggct tgaagtcgag
      721 cctgctgccg tcggcgctcc acgaggcggt gccggctatc ggcggctcga agctgagcgg
      781 gttgacgccg cgcagcgcgt cggcgtctcg gccggagctg agcaccacgc tcaggtcggc
      841 gaaacgcgag acgatgccgt cgctatgcgc gacgacgaag gcgggatccg gagccctatc
      901 ctccttgccg acgcggctcg gcgcgcaagc ggccgacagg gtagaaacga gcacgacggc
      961 cgcgatggcg gccttgaagc gtagcgcatg gttcaggctc atgagacgtt ccttcttagc
     1021 cggggcgccg tcgatggttg gaccggcgct ccctagtata cgtcgtcggt tcagttccgt
     1081 caggcttcag cggaggctat aaggccccgc atccgctctt cgagcgcgac ccagacctcg
     1141 tcggcgcggc cctccccgtc gaccgagacg agcgtgccgc gacgcttata gaagtcgacg
     1201 aggggcgccg tctggcggta gtaatgctcg agtcggctac gaacggaggc ctccccgtca
     1261 tcatcccgga catagagctc gccgccgcag ccgtcgcaga caccttcctc ggctggaggc
     1321 atcaggctga catggaagct gcgcccgcag ccgcggcaca tccgccgccc ggacaggcgc
     1381 tcgacgacct tggcgtcgct cacctcgagg tcgatcacga agtcctccgg gcagaacccg
     1441 gccagggcct cggcctgggc cagggtcctc gggaaaccgt cgagcaacca gcccctcgcc
     1501 gcgtcggcct cgccgaggcg ctcgcgaacg aggccgatgg tcagctcgtc ggacacgaga
     1561 ccacccgatt cgatgacggc cttgaccctg agcccgagcg gcgttccggc gcgcatggcc
     1621 gccctgaaga tttcgcccgt cgagatatgc ggcaggccga acgccaccgc cgccctggcc
     1681 gccatggtgc ctttacccgc tccaggcgct ccgaagaata cgaatttcat agtcgacccg
     1741 ctccttgcgc gctatatgcc agcatcctat ctcaagtcgc gggcgcgcgc taggccgtac
     1801 ggctaccgag tccggccggg gccctcgtcc aggccgcaca cgctccacgg cttgttcagg
     1861 ttgtaccgga acagcacctc catgtgcctg atcaccgatt cggtagggaa cacgattgaa
     1921 aacgcccgat tctccctgaa atccgtgctg gatggcgtac cgatgggctc atcgtcgacc
     1981 tggataggta gtccgcggct cctgatcgcc tcgacgtcgg atgccgagac tggagagcct
     2041 tccctggtca cgacgaaggc ctcgtacgtg aaatcgacgc gcctacgttc agattcctgg
     2101 cgccatgcgt ttaggtcctc gggcagcatc gacgccgcga acggtagcgt atccgaaccc
     2161 aggaggtggg tcatcgagac ccgagcgccg gggaacatcg cgatgaagcg cctgacgatg
     2221 ccgatggtgt cggctccctc gcctatgtcc agaggcacga tcagcgggtc gaagactccg
     2281 gctacctgca tcgatagaag ctcgtaccga tagcgatagt cgctcttcct gggcttgctc
     2341 gggttatcgt ggccctcggg cacgacgaac acgacgggcg ctccggcggc gtccgaggcg
     2401 aggaacctga gcgccgtggc caggtgcgtc atctggaagg ggtcgaagga gccgatgaat
     2461 aacgcggcgc ggatctcgta acgcgggaag cgtatcacct ggggaaaatc ggacaggtcc
     2521 accggctcga gccggccatc cgctatgcga tcgtcgatcc gccgcaacgc ttcgaggtag
     2581 aaagtcctgt agtacgagaa gtcgcccctg tcggtggcgc tcaggtagga atacgcgccg
     2641 aggcgatcga acacctcctc ggtgcgggcg gccgcgcgag cgatgaggtc ggtccgcatc
     2701 gggccgctc
//
LOCUS       DAOOLN010000123         2704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_46229_length_2704_cov_2.935825, whole genome shotgun sequence.
ACCESSION   DAOOLN010000123 DAOOLN010000000
VERSION     DAOOLN010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46229_length_2704_cov_2.935825"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PLW80_02300"
     CDS             complement(<1..675)
                     /locus_tag="PLW80_02300"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HPB65361.1"
                     /translation="MRQFVLPGSWDGGPVCELEGRDARRLLTVLRLSPGDSFPAIGPD
                     GAPYSCRIESAGRGRLTLSVAPGALASAGYKPDVRAGRPASLVAGLAAGLAAGLAAGP
                     SRDEAVAVPRLILAVGLLKGSKLDEVARIAAEAGVEAIVPLCTARALPRDHADGRIER
                     LRRVVAEAIGQSGSGVATRVEAPTTVAGLCVARPGRSPGRLGLFFHEAPLAQSSIHRY
                     CTDVPDE"
     gene            complement(683..2182)
                     /locus_tag="PLW80_02305"
     CDS             complement(683..2182)
                     /locus_tag="PLW80_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPB65362.1"
                     /translation="MKNPNDRGRDRAAWGVVAVVSAFCVALSFSAPSAYAQDSKAESQ
                     RYFSIFQSAFNFILENYVEEVDPKVLYEGAMQGMFDSLGDPYSSFLTDAMMSDLNDTT
                     TGRFGGIGLYISKPLADAKAPDRRLFVEVVSPIEDTPGWRAGIMPGDQITHIDGEPTE
                     PLTMDEVLGRLRGEPGSTVTITLLRGAGLVFDERITRAVIEVPTVKRAMLPGGAGYLR
                     IIEFTPQTVSRVSEAIEWFKSQRYTSLVIDLRNNPGGLLQSVIQIADLFLDRGVIVST
                     KSRNRYENAVYQAKPPLAVPKDLRVVVLINRGSASASEILAGALKDQKRAYLIGEKSY
                     GKGSVQQVLPIGETGFKLTMSRYYTPSDANIDKVGIAPDLEVKEPELSPEEEESLSGL
                     IERDVFAAFAAANPNASPQQVDQYVEGLRAAGSPVSERVLKKLVRNQLERTSISPVYD
                     LEYDLQLNAALDALDDDDFWSRISEAYSVMELQALAVGAQAAGAVTAGR"
     gene            2323..>2704
                     /locus_tag="PLW80_02310"
     CDS             2323..>2704
                     /locus_tag="PLW80_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HPB65363.1"
                     /translation="MADFLADSDFELYRKLIYDESGIHFSATNRSILESRLRERLREN
                     KLQTPQEYYAILVKDKEELKVLLDSVTTNLTRFFRNQAHFDAIEHYVVPALLKLRQAE
                     RKIRVWSAGCSTGEEPYTIALLLKE"
BASE COUNT          470 a          897 c          877 g          460 t
ORIGIN      
        1 ctcgtccggc acgtcggtac aatagcgatg gatcgacgac tgcgcaaggg gagcttcgtg
       61 gaagaacagc ccgagccggc ccggcgatcg acccggcctg gccacgcata aacccgcgac
      121 ggtcgtcggc gcctcgacgc gcgtggccac gccggaaccg gactggccga tagcttcggc
      181 caccacgcgc ctgagcctct cgatacggcc gtcggcatgg tcgcgcggaa gcgccctcgc
      241 ggtgcataag ggtacgatgg cctcgactcc tgcctcggcg gctatcctgg ccacctcgtc
      301 gagtttggag cccttgagca ggccgacggc caggatcaac cgcggtacgg ctacggcctc
      361 gtcacggctc ggaccggccg cgagaccggc cgcgagaccg gccgcgagac cggccacgag
      421 acttgccggc cgaccggccc tgacatccgg cttgtagccc gcggatgcga gggccccggg
      481 agcgaccgac agggtcagcc gaccgcgccc ggcggactcg atccggcacg agtacggcgc
      541 gccgtccggg ccgatggccg ggaacgagtc gccgggagac agtctgagca cggtcaggag
      601 gcgccgcgca tcgcggccct cgagctcgca gacgggaccg ccgtcccagc tcccgggcag
      661 gacgaactgg cgcatcccct ggtcagcggc ccgcggtgac cgcgccggcc gcctgggcgc
      721 cgacggccag tgcctgtagc tccatcaccg agtacgcttc cgatatgcgc gaccagaaat
      781 catcgtcgtc gagcgcgtcg agcgctgcgt tcagctgcag gtcgtactcg agatcgtaga
      841 ccgggctgat cgacgttcgc tcgagctggt tccgtaccag cttcttgagc acacgctcgg
      901 acaccggtga gcccgcggcg cggagcccct cgacgtactg gtcgacctgc tgtggcgagg
      961 cgttcgggtt ggccgccgcg aaggccgcga agacgtcgcg ctcgatcagg ccggacaggg
     1021 actcttcctc ctcggggctg agttccggct ccttgacctc gaggtcaggc gcgatgccga
     1081 ccttgtcgat gttcgcgtcg ctcggcgtgt agtacctgga catggtcagc ttgaagccgg
     1141 tctcgccgat cggcaacacc tgctgcaccg agcccttgcc gtagctcttc tcccctatca
     1201 ggtaggcgcg cttctggtcc ttgagggcgc cggccaggat ctccgaggcg ctcgccgaac
     1261 cgcgattgat cagcacgacg acgcgcagat ccttgggcac cgccaggggt ggcttggcct
     1321 ggtaaacggc gttctcgtag cggttgcgcg acttggtcga cacgattact ccgcggtcga
     1381 ggaacaggtc cgctatctgg atgactgact ggagcaggcc gccggggttg ttgcgcaggt
     1441 cgatgacgag tgacgtatac cgctgcgatt tgaaccactc gatagcctcg ctgaccctgg
     1501 agacggtctg cggggtgaac tctatgatgc gcaggtagcc ggcgccgccg ggtagcatcg
     1561 cccgcttgac cgtgggcacc tcgatgaccg ccctggtgat cctctcgtcg aacacgaggc
     1621 cggcgccacg gagcagggta atggtcaccg tcgagcccgg ctcgccgcgt agccggccca
     1681 gcacctcgtc catagtcagc ggctcggtcg gctcaccgtc tatatgagtg atctggtcgc
     1741 cgggcatgat gcctgcgcgc cagccgggcg tgtcctcgat gggcgagacg acctcgacga
     1801 atagccgacg gtctggagcc ttggcgtcgg ccaggggctt cgaaatatag aggcctatac
     1861 cgccgaacct gccggtggtc gtatcgttca ggtcgctcat catcgcgtcc gtcaggaacg
     1921 acgagtaggg gtccccgagc gagtcgaaca tgccctgcat ggcgccctcg tacaggacct
     1981 tcggatcgac ctcctcgaca tagttctcga ggatgaagtt gaacgcgctc tggaatatgg
     2041 agaaatatcg ctggctctcg gccttggaat cctgggcata ggcggacggc gcgctaaagg
     2101 acagagcgac gcagaacgct gagactaccg cgacgacgcc ccaagcggcc cggtcgcgac
     2161 ctcgatcatt cgggttcttc atggcctaac tatcctcttt ggcgggccgg cagtcaaccg
     2221 cctttcgagg cccttctatt gacagcctag acttctcggc actagacttc gaccgagcga
     2281 gggccgcgag ctctccatgc acgttcggcg gagaggcaag cgatggcgga tttcctggct
     2341 gattcagatt tcgagctgta tcgcaagctg atatacgacg agagcggcat acacttctcc
     2401 gcgacgaacc gttccatcct cgagagccgg ctccgcgagc gcctccgcga gaacaagctg
     2461 cagacgccgc aggaatacta cgccatccta gtcaaggata aagaggagct caaggtgctc
     2521 ctcgactcgg taacgaccaa cctgacccgc tttttccgca accaggctca tttcgacgcc
     2581 atcgagcact acgtggttcc cgccctcctc aagctgcgcc aagccgagcg caagatacgg
     2641 gtctggagcg ccggttgctc gactggagag gagccgtata ccatcgcgct gctcctcaag
     2701 gagc
//
LOCUS       DAOOLN010000124         2704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_46230_length_2704_cov_2.795017, whole genome shotgun sequence.
ACCESSION   DAOOLN010000124 DAOOLN010000000
VERSION     DAOOLN010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46230_length_2704_cov_2.795017"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="PLW80_02315"
     CDS             <1..727
                     /locus_tag="PLW80_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPB65364.1"
                     /translation="ALSYAELSGGAFDPTIGPVVKLWNIGMDGERIPAPEEIAEALPL
                     VDASAVAVDDAASTVFLPRAGMMLDLGAIAKGYAADEVARVLLEHRVKAAVVDLGGNV
                     KVVGRKPDGSPWRIGIQDPFDDRGSRIGIAELEGGATVVTSGVYERYFIGDDGAHYHH
                     ILDPATGYPARNGLVSVTVISSSSIDADGLSTTLFSLGLERGMALVETLDHVKPSSST
                     RTGASTRAPAPGSCSSSIILRSP"
     gene            complement(733..1239)
                     /locus_tag="PLW80_02320"
     CDS             complement(733..1239)
                     /locus_tag="PLW80_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPB65365.1"
                     /translation="MNQITINGVAHTVSDKERPLSLLEYLRERRFLTGTKNGCEKGLC
                     GSCTVVVDGKALRSCRIPVEKALGASVVTIEGMEGPGGEPHPIQQAFMDAGAIQCGFC
                     TPGMVMSAYALLLRDPDPSRDDIRKALKGNLCRCAGYQQIVDAVRLAAKVMREQALPV
                     EAGCLPRR"
     gene            complement(1236..>2704)
                     /locus_tag="PLW80_02325"
     CDS             complement(1236..>2704)
                     /locus_tag="PLW80_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPB65366.1"
                     /translation="WSFRESYKRHPYRMRYKVGVSSEGRVLAVKATMHADSGAYLSVT
                     PWVTWRSTAQCFGPYVVDAIHADVYGVATNNVFTGAMRGFGAPQVNFAVEQLMDMAAH
                     ELKIHPLGMRRINMVKQDSVTVTGQKLDGHVVSMEDVMNRVVHEIGYRKKYEHCSFGT
                     PSEEGSDELYGIGLAISYRGASLGAEGMDFCSCIINGQYDGSILLETGIHENGQGAES
                     AMTLILAEELGVDLSRVRYKRSSTSSIPDSGTTVATRGTIMGGSAVVVAARQYRELLS
                     RHLADRLRCRPEDVTFHDDKIWGLNYEYSLSWEEAMRELFLQRVTPYAFGTFQAPPVS
                     WDDTTGQGDAYFTYVYSCQAVELTVDRKTGKIRLRSIVAGHDIGKAVNRALLLGQVYG
                     GVTQGVGMALMEDFAYEGGRPVCENFDSYLLPRATDLPEMTAVIVENHDPASLTGAKG
                     IGEPALELIAPAIANAVYNATGVRCRDLPIRLRPEDLA"
BASE COUNT          418 a          892 c          924 g          470 t
ORIGIN      
        1 ggcgctatcg tacgcggagc tgtcgggcgg cgctttcgac ccgaccatcg gccccgtcgt
       61 caagctgtgg aacatcggca tggacggcga gcggatcccc gctccggagg agatagccga
      121 ggccctgccc ctcgtcgacg cgagcgccgt agccgtggac gacgcggcca gcaccgtctt
      181 cctgcctcgg gccggcatga tgctcgacct cggcgccatc gccaagggct acgccgccga
      241 cgaggtggcc cgcgtcctgc tcgagcatcg ggtcaaggcc gccgtcgtcg acctcggcgg
      301 caacgtcaag gtggtcggcc ggaagccgga cggctctccc tggcgcattg gcatccagga
      361 tcccttcgac gaccgaggct cgcgcatcgg catagccgag ctcgagggcg gcgcgacggt
      421 cgtgacgagc ggcgtctacg agcgctattt catcggggac gatggagcgc actaccacca
      481 tatattggat ccggccaccg gctatccggc gaggaatggc ctcgtctcgg tcacggtgat
      541 cagctcgtcg tcgatcgacg ccgacggcct gtcgacgacg ctgttctccc tgggcctgga
      601 gcggggcatg gccctcgtcg agacgctcga ccacgtcaag ccgtcttcat cgacgaggac
      661 cggcgcgtct accagagctc cggcgccggg aagctgttca agctcgataa tcctgcgttc
      721 accctgacag cgctaccgtc tcggtaggca gccagcctcg accggtagcg cctgctcgcg
      781 catgaccttg gcggctagcc ggaccgcgtc gacgatctgc tgatagccgg cacagcggca
      841 gaggttgcct ttgagggcct tgcggatatc gtcgcggctc gggtcggggt cgcgtagcag
      901 gagagcgtag gcgctcatca ccatgccggg cgtgcagaag ccgcactgga tggctccggc
      961 gtccatgaag gcctgctgga tcgggtgcgg ctcaccgccg gggccctcca tgccctctat
     1021 ggtcaccacc gacgcgccga gcgccttctc cacgggaatc cggcaggatc ggagggcctt
     1081 accgtcgacg acgacggtgc aggagccgca gagacccttc tcgcagccgt tcttggtgcc
     1141 cgtcaggaag cggcgctcgc gcaggtactc gaggagcgac agcgggcgct ccttgtcgct
     1201 caccgtatgc gcgacgccgt tgatcgttat ctggttcacg cgaggtcctc cggtctgagt
     1261 cgaatcggca ggtcgcggca acgcacgccg gtggcgttgt acacggcgtt ggcgatggcc
     1321 ggcgctatca actccaacgc cggctccccg atgcccttgg ctccagtcag ggaggctggg
     1381 tcatggttct cgacgatcac ggcggtcatc tccggcagat cagtcgccct cggcagcagg
     1441 tacgagtcga aattctcgca gacgggcctg ccgccctcgt aggcgaagtc ctccatgagc
     1501 gccatgccga cgccctgggt cacgccgccg tatacctggc cgagcaagag ggcccggttg
     1561 accgccttgc cgatgtcgtg gccggccacg atgctccgta gtctgatctt gccggtcttc
     1621 cgatcgaccg tcagctcgac cgcctggcac gaatagacgt aggtgaagta cgcgtcgccc
     1681 tgaccggtcg tgtcgtccca gctcaccggc ggagcctgga aggtgccgaa ggcgtagggg
     1741 gtgacgcgct gcaggaacag ctcgcgcatc gcctcctccc agctcaggct gtactcgtag
     1801 ttcaggcccc agatcttgtc gtcgtggaag gtgacgtcct cgggcctgca gcgcaggcgg
     1861 tcggccaggt gccgcgacag gagctcgcgg tactgcctgg ccgcgaccac gacggcggag
     1921 ccgcccatga tggtgccgcg cgtggcgacg gtggtgcccg agtcggggat ggagctggtc
     1981 gacgagcgct tgtaacggac gcggctgaga tcgacgccga gctcttcggc gaggatcagc
     2041 gtcatcgccg actcggcgcc ctgcccgttc tcgtggatgc cggtctccag caggatggag
     2101 ccgtcgtact ggccattgat gatgcaggaa cagaagtcca tgccctcggc gccgagggat
     2161 gcgccccggt agctgatggc caggcctatg ccgtacagct cgtcggagcc ctcctccgac
     2221 ggcgtcccga acgagcaatg ctcgtacttc ttgcggtagc ctatctcgtg gacgacgcgg
     2281 ttcatcacgt cctccatgct gacgacgtgg ccgtccagct tctgcccggt gacggtgacc
     2341 gagtcctgtt tgaccatgtt gatgcggcgc atgccgagcg ggtggatctt gagctcgtgg
     2401 gctgccatgt ccatgagctg ctcgacggcg aagttgacct gcggggcgcc gaagccgcgc
     2461 atggcgccgg tgaatacgtt gttcgtcgcg acgccgtaga cgtcggcgtg gatggcgtcg
     2521 acgacgtagg ggccgaagca ctgggccgtg gagcgccagg tgacccaggg cgtcaccgac
     2581 aggtaggcgc cggagtcggc gtgcatcgtc gccttgaccg cgaggacgcg gccctcgctc
     2641 gatacgccta ccttgtagcg catgcggtac ggatggcgct tatagctctc gcggaacgac
     2701 cact
//
LOCUS       DAOOLN010000125         2697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_46401_length_2697_cov_2.036336, whole genome shotgun sequence.
ACCESSION   DAOOLN010000125 DAOOLN010000000
VERSION     DAOOLN010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46401_length_2697_cov_2.036336"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLW80_02330"
     CDS             complement(<1..397)
                     /locus_tag="PLW80_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HPB65367.1"
                     /translation="MAKPLEGVRVLDLTRVLAGPFCTMVLSDLGAEIIKVEVPGTGDD
                     SRAFGPFKNGQSLYFVNINRGKKSVAIDLKTDAGKKLLVDLAKECDVIVENFRPGTME
                     KLGLGWDVLKAANPRLIYAAVSGFGHTGPD"
     gene            complement(549..1421)
                     /locus_tag="PLW80_02335"
     CDS             complement(549..1421)
                     /locus_tag="PLW80_02335"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HPB65368.1"
                     /translation="MKMIDLTIPLGIGTPPWPTYEPLEVKYFKRLAPNGANGQLVTHS
                     NHIGTHLDGEIHFYTPGKDIASLDMDFLVHEGAVVDLSDIAGDYDVYTSKMVEDRVEV
                     KEGDILIIHTGFHHYGWDQPTGDEIRYMIKHPGPDREFAEWAKKKKLRWIGVDCGSAD
                     HPMNTKIRDWMPKQAAECDAHMKKKYGKGLSEVFADDKYQLMHIEMFNVGIIHAECLG
                     GDIDLLLNQRATIGCFPWRFVDGESSIARIVAMVDDERYEKLQAKKAKCELTKFGDIA
                     GAKAAWLHEEARKR"
     gene            complement(1574..>2697)
                     /locus_tag="PLW80_02340"
     CDS             complement(1574..>2697)
                     /locus_tag="PLW80_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HPB65369.1"
                     /translation="PPLDWERASGPMKGALIGAMLFEGMADTPEEAEAKLAAGEGITL
                     EPCHHRDAVGPMAGVVSPSMWMYELHDPVHDNRSWCSLNEGLGKVLRYGAYGPEVIER
                     LQWMNSVLGPLLQQAIRKAERIDIKAIVAQMLQMGDEGHNRNRAGTSIIIRELAPHIV
                     MLDYPKEKLSRALAFLNANDHFFLNLTMPSAKCSVDAAAGIEGSTIITTMARNGTEFG
                     IRLSGLPGRWFTGPSGIVDGLYLPGFGPEHAARDIGDSVITETSGIGGFAMAAAPAIV
                     KFVGGSPRDAIDVTMRMYEITNAESEAYRIPALDFRGTPTGIDVRKVVETGILPAINT
                     GIAHKDPGVGMVGAGLVKPPENAFRDALIAFAEKYAKKL"
BASE COUNT          440 a          846 c          843 g          568 t
ORIGIN      
        1 agtccgggcc ggtgtggccg aagcccgaga cggcggcgta gatcaggcgc gggttggccg
       61 ccttgaggac gtcccagccg agtccgagct tctccatcgt acccggcctg aagttctcga
      121 cgatcacgtc gcattccttg gccaggtcga cgagcagctt cttgccggcg tcggttttga
      181 gatcgatcgc gacgctcttc ttgccgcggt tgatgttgac gaagtagagg ctctggccgt
      241 tcttgaacgg cccgaaggcg cgcgaatcgt cgccggtgcc cggaacctcg accttgatga
      301 tctccgctcc cagatccgaa agcaccatcg tgcagaacgg tcctgccagt acccgggtaa
      361 gatcgaggac gcgtacgccc tccagcggct ttgccatacg tgctcctggt cattgataac
      421 cgataaagaa taccacggag gcacggaggc acggagaatg ataagaaaag ataaggacca
      481 ggttttccta gcctgttctt gaatccttct tatctccgct tctccgtgcc tccgtggcca
      541 tattcgcctt agcgcttgcg ggcttcctcg tggagccagg cggccttggc gccggcgatg
      601 tctccgaact tcgtcaactc gcacttggcc ttcttggcct gcagtttctc gtagcgctcg
      661 tcgtctacca tggccacgat gcgcgcgata ctcgactcgc cgtcgacgaa gcgccacggg
      721 aagcagccta tcgtcgccct ctggttcagg agcaggtcga tgtcgccgcc caggcactcg
      781 gcgtggatga tgccgacgtt gaacatctcg atgtgcatca gctggtactt gtcgtcggcg
      841 aatacctcgg atagtccctt gccgtacttc ttcttcatgt gggcgtcgca ctcggcggcc
      901 tgcttgggca tccagtcgcg gatcttcgtg ttcatcgggt gatcggccga gccgcagtcc
      961 acgccaatcc agcgcagctt cttcttcttg gcccattcgg cgaactcgcg gtccggcccc
     1021 gggtgcttga tcatgtagcg gatctcgtcg ccggtcggct ggtcccagcc gtagtggtgg
     1081 aagccggtat ggattatcag gatatcgcct tccttgacct cgacgcgatc ctcgaccatc
     1141 ttggaggtgt acacgtcgta gtcgccggcg atgtcggaca ggtcgacgac ggcgccctcg
     1201 tgcacgagga aatccatgtc caggctcgct atatccttgc ccggcgtgta gaagtgaatc
     1261 tcgccgtcga ggtgcgtgcc gatatggttc gagtgggtga cgagctgccc gttggcgccg
     1321 ttcggcgcga gacgcttgaa gtacttgacc tcgagcggct cgtaggtggg ccagggcggg
     1381 gtgccgatgc cgagcggaat cgtcaaatcg atcatcttca taagaacctc ctgtagatta
     1441 actgaacgaa tcagaccacg gagccactga gtaccgtaat gacgaaagac gcgaagcgtg
     1501 atggtcgatt cgcctgtatc tttaatcttc cgtcattctt accgactccg tgcctccgtg
     1561 gttctcttgt cgattatagc ttcttcgcgt acttctcggc gaaggcgata agggcgtccc
     1621 tgaaggcgtt ttcgggtggc ttgacgaggc cggcgccgac catgccgacg ccggggtcct
     1681 tgtgagcgat gccggtgttg atcgccggca gtatgcccgt ctcgaccacc ttgcggacgt
     1741 cgatgcccgt cggggtgccc ctgaagtcga gcgccggtat gcggtaggcc tcgctctcag
     1801 cgttcgtgat ctcgtacatc ctcatagtca cgtcgatcgc gtccctgggc gaaccgccga
     1861 cgaacttgac gatagccggg gcggccgcca tcgcgaagcc gcctatgccg gaggtctcgg
     1921 tgatcaccga gtcgccgata tcgcgggccg cgtgctctgg gccgaaaccc ggcagataca
     1981 gcccatcgac gattccggac ggcccggtga accagcggcc cggtaggccg gagagcctga
     2041 tgccgaactc ggtaccgttg cgggccatcg tcgtgatgat cgtcgagccc tcgatgccgg
     2101 cggccgcgtc gacggagcac ttggcgctcg gcatcgtcag gttcaggaag aagtggtcgt
     2161 tggcgttcag gaaggcgagc gccctcgaca gcttctcctt ggggtagtcg agcatgacga
     2221 tgtgtggcgc cagctcccgg atgatgatcg aggtgccggc gcggttgcgg ttgtggccct
     2281 cgtcgcccat ctgcagcatc tgtgcgacga tcgccttgat gtcgatccgc tcggccttgc
     2341 ggatggcctg ctgcagcagc ggcccgagga ccgagttcat ccactgcagt cgctcgatga
     2401 cctcgggacc gtaggcgccg tagcgcagca ccttgcccag gccctcgttg agcgagcacc
     2461 acgagcggtt gtcgtggacc gggtcgtgca gttcgtacat ccacatgctc gggctgacga
     2521 caccggccat cggacccacc gcatcgcggt ggtggcacgg ttcgagtgtg atgccctctc
     2581 ccgcagcgag tttcgcctcg gcctcctcgg gagtgtcggc catgccctcg aagagcatcg
     2641 ccccgatcag cgcacccttc atcggcccgc tcgcgcgctc ccaatccagc ggcggcc
//
LOCUS       DAOOLN010000126         2690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_46549_length_2690_cov_2.381025, whole genome shotgun sequence.
ACCESSION   DAOOLN010000126 DAOOLN010000000
VERSION     DAOOLN010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46549_length_2690_cov_2.381025"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1669
                     /locus_tag="PLW80_02345"
     CDS             <1..1669
                     /locus_tag="PLW80_02345"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HPB65370.1"
                     /translation="LIRAEDFKELEDRFYRDLEFGTGGLRGVIGGGYNRMNSLVVRRA
                     TQGMCDYLNATVGGKSLSACVAYDSRRYSDVFALDTALIFAANGIKAYLFTSLRPTPE
                     LSYAIRKLGADTGVVVTASHNPPAYNGYKAYWNDGAQVTSPHDTGIIDRVNAVKTIKS
                     MPRDEAIAKGLLVMIDKEIDDPYVAMVKSRLFRPELIKTMAKDVKVVYTPLHGTGAMP
                     FERVMGELGLSVVTVPEQREPNGDFPTVSYPNPEESAALKMALELGAKVKADVVMATD
                     PDADRLGIAVPDGKGGFALITGNQLGALHADYILLSLKELGRMPKRPATIKSIVTTEL
                     QARIARAYGAECHDCLTGFKWIADTMRRFERDGLDFVYATEESYGYLVEDEVRDKDGI
                     SAAAMTAEMTMYWRSKGKSLLQRLDELFVEHGFHEERGVNKYFEGPTGMDVMRGIMDA
                     YRADQPKTLGGIPVERIRDIKTGKAWSVAGGPGKAETVDLPTSDVIQWYLEDGTLVTV
                     RPSGTEPKIKCYVLARTAVGPEGLAAARKASNAKVDAILADIKKVIG"
     gene            1666..2376
                     /locus_tag="PLW80_02350"
     CDS             1666..2376
                     /locus_tag="PLW80_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPB65371.1"
                     /translation="MSPYGRRGYGSRPGEGRSSGRAGTGPSRRGDGAHGASRLSIDDV
                     DALFSAVGRVRFVAFDFETTGLSPERDRVVEIGAVAFRMALDESGPDGQPWRAIEDGS
                     FETLIHPGRPIPPEVSAIHGIDDLAVSGAPSFAEAAARFLPFIDGSILVAHNAPFDLG
                     FLRAETARAGIENPPNPAYDTIAIAKTAISGLPSYSLKSLASAFGIVQTAAHRGGDDA
                     KVCMDLFSRCINLIYTSK"
BASE COUNT          501 a          922 c          815 g          452 t
ORIGIN      
        1 gctgatccgg gcggaggact tcaaggagct cgaggaccgc ttctaccgcg acctcgaatt
       61 cggcaccggt ggccttcgcg gcgtgatcgg cggcggctac aaccgcatga actcgctcgt
      121 ggtccgccgc gcgacccagg gcatgtgcga ctacctgaac gcgaccgttg gcggcaagag
      181 cctgtccgcc tgcgtcgcct acgactcgcg ccgctactcg gacgtgttcg cgctcgacac
      241 ggctctgatc ttcgcggcca acggcatcaa ggcctacctg ttcacctcgc tacggccgac
      301 ccccgagctg tcctacgcga tcaggaagct cggcgccgac acgggcgtgg tcgtcaccgc
      361 gagccacaac cccccggcct acaacggcta caaggcctac tggaacgacg gcgcccaggt
      421 cacctccccc cacgacacgg gcatcatcga ccgggtgaac gccgtgaaga cgatcaagtc
      481 gatgcccagg gacgaggcca tcgccaaggg cctcctcgtc atgatcgaca aggagatcga
      541 cgacccgtac gtcgccatgg tcaagtctag gttattcagg cccgagctga tcaaaaccat
      601 ggccaaggac gtcaaggtgg tctacacccc gctccatggc accggggcca tgcccttcga
      661 gcgcgtcatg ggcgagctcg gtctgtcggt cgtcaccgtg ccggagcaac gcgagccgaa
      721 cggggacttc ccgactgtct cctacccgaa cccggaggag tcggccgctc tcaagatggc
      781 gctggaactc ggcgcgaaag tcaaggccga cgtcgtcatg gccaccgacc ccgacgccga
      841 ccggctcggc atcgctgtgc cagacggcaa aggcggcttc gccctgatca cgggcaacca
      901 gctcggcgcc ctccacgccg actatatcct cctgtccctg aaggagctcg gccgcatgcc
      961 caagcgaccg gcgacgataa agagcatcgt caccaccgaa ctgcaggcgc gcatagcccg
     1021 cgcctacggc gccgagtgcc acgactgcct gaccggtttc aagtggatag ccgacacgat
     1081 gcgccgcttc gagcgcgacg ggctcgattt cgtctacgcc accgaggaga gctacggcta
     1141 cctcgtcgag gacgaggtcc gcgacaagga cggcatctcg gccgccgcga tgaccgccga
     1201 aatgaccatg tactggcgct ccaagggcaa gagcctcctc cagcgcctgg acgagctgtt
     1261 cgtcgagcac ggcttccacg aggagcgcgg cgtcaacaag tatttcgagg ggccgaccgg
     1321 catggacgtg atgcgcggca tcatggacgc ctaccgcgcc gaccagccca agaccctcgg
     1381 cggcataccg gtcgagcgca tccgcgatat caagaccggc aaggcctgga gcgtcgcagg
     1441 cggccccggc aaggccgaga cggtcgacct gccgacgagc gacgtgatcc agtggtacct
     1501 ggaggacggc accctcgtca ccgtgcgccc gagcggcacc gagcctaaga tcaagtgcta
     1561 cgtgctcgcc cgcaccgcgg tgggcccgga ggggctcgcc gccgccagga aggcttcgaa
     1621 tgccaaggtc gacgctatcc tggccgacat caagaaagtg atcggttgag tccctacggc
     1681 cgtcgcggct acggctcccg tccgggcgag gggcgatcct ccggccgggc gggcaccggc
     1741 ccgtcgaggc gcggcgatgg cgcccatggc gcctcgaggc tttcgatcga cgacgtcgac
     1801 gccctgttct ccgccgtcgg acgcgtgcgc ttcgtcgcgt tcgacttcga gacgacgggc
     1861 ctgtccccag agcgcgaccg cgtcgtcgag ataggcgccg tcgcgttccg gatggccctg
     1921 gacgagtccg gaccggacgg ccagccgtgg cgcgcgatcg aggacggctc gttcgagacc
     1981 ctgatccatc cgggccggcc gatacctccg gaagtcagcg ccatccacgg catcgacgac
     2041 ctggccgtct ccggggcccc gagcttcgcc gaggccgccg cccgcttcct gcccttcatc
     2101 gacggctcga tactcgtcgc ccataacgcc ccgttcgacc tcggcttcct cagggcggaa
     2161 accgcccgcg ccgggattga aaacccgccg aatccggcgt atgatactat agctatagcg
     2221 aagacggcca tctcgggcct gcccagctat tcgctcaagt cgctcgcttc ggcgttcggc
     2281 atcgtgcaga cggcagccca ccgcggtgga gacgacgcaa aagtctgcat ggatttgttt
     2341 tcccgctgca taaacctaat ctacacaagc aaataaaaca attcgacaat ttcgtcgtat
     2401 cgacgtattt gtcgaattgt tttctccatc cttcaaacta cactttatca tggtacatat
     2461 ttgtcgatat cgacaaatag gtcgctgtgt gatattgtat taaatagaag acccaagttt
     2521 tcgcctgccg gcattgtgcc gatagtagaa tcgggcgcat gcgcgccgat accgtccgtg
     2581 tagggagttg gtatgaaaac gacgactggg tcgctcgtaa tccgcggcgc gctagcgctt
     2641 tctgtgcttt tcatggcctc cctggccgct tcctgcaaga gtcccctctt
//
LOCUS       DAOOLN010000127         2688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_46601_length_2688_cov_3.057729, whole genome shotgun sequence.
ACCESSION   DAOOLN010000127 DAOOLN010000000
VERSION     DAOOLN010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46601_length_2688_cov_3.057729"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..158
                     /locus_tag="PLW80_02355"
     CDS             <1..158
                     /locus_tag="PLW80_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB65372.1"
                     /translation="AYIVKFTAEKHADEGAMMAVAVIASLPVFVLFFTLSKYFLGGSA
                     VYQAGKE"
     gene            155..760
                     /locus_tag="PLW80_02360"
     CDS             155..760
                     /locus_tag="PLW80_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPB65373.1"
                     /translation="MSLFIFDMGGVMVGSFDVLPEAALRLGLPETELRRLAAPDLDEL
                     MAGRMAADDYWARFSVASGLSVGEDYWSTLFAPSLDPVMVGLARSLRRRGRVVCGTNT
                     IASHYDYLRAAGMYDVFDAVYASHLMGVCKPDPDFWRLILEAEGVAAPDAVFVDDMAE
                     NAEAAGALGLRAHLFDGVAGLEAFLDRLDDEPEAADRRLEA"
     gene            757..1821
                     /locus_tag="PLW80_02365"
     CDS             757..1821
                     /locus_tag="PLW80_02365"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPB65374.1"
                     /translation="MTRRSAHGRIGLVVVTMAGLAVAASVALASCARADPVKAVPEAD
                     LVVFSSHPDELAKTIIDEFRERTGLRVRLVLDGTGALLSRLSAGERADVLWGGGAESL
                     VANLGLFQPYLSPERDAVPAALKDEDGYWTGLTVLPMVIMVNARLVPPEAVPAGWSDL
                     LAPELAGGVAFANPVSSASSYTILRTMMVAARARAGGDQDADWRFVEGFIDAIGGSTL
                     SESAMVYRVVAAGEYLVGLTYENAGAESMRLGSDVRIVYPADGTSTVPDGVAILRDAA
                     HDEAARRFIDFVLGHDVASVVSTRFKRRSARVDVPAPDGLPPLESVRLLDYDFDAAVT
                     EREATMARFTTMLQEAGQRR"
     gene            complement(1818..2642)
                     /locus_tag="PLW80_02370"
     CDS             complement(1818..2642)
                     /locus_tag="PLW80_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB65375.1"
                     /translation="MRRLVIAEDEELARRELETTMPWERWGYMLVGSAEDGLHAWDLA
                     VERRAEVVLTDIRMPGLDGLALMRKAEAELAPLERPLFIIVSGHADFDYAKEAVRLGA
                     FDYLIKPVDDGELEDAMLRAAKRAGERERLAGLERAATADQAYGLLRDLTRERTAESG
                     DSYVEAAIADLGERFMTDLSADSVAGRLGISGDHLSRLLKRATGLTFNEYLTRLRMKR
                     AMELLRDPTVRVGEVADLSGYRDARYFSTLFRRVIGMTPSEFRSGRIRRDTGDPSP"
BASE COUNT          393 a          929 c          910 g          456 t
ORIGIN      
        1 cggcctatat cgtcaagttc accgcggaga aacacgccga cgaaggcgcg atgatggccg
       61 tggcggtcat cgcctcgcta ccggtcttcg tgctattctt caccctatcc aagtatttct
      121 tagggggatc ggccgtctac caggccggta aggaatgagt ctcttcatct tcgacatggg
      181 cggcgtgatg gtcggctcgt tcgacgtact acccgaggcc gcgctaaggc taggcctgcc
      241 cgagaccgag ctacggcgcc tggcggcgcc cgacctggac gagctgatgg ccgggcgcat
      301 ggccgccgac gactactggg cgcggttttc ggtcgcatcc gggctgagcg tcggcgagga
      361 ctactggagc acgctgttcg cgccatcgct cgacccggtg atggtcggcc tggcccgtag
      421 cctacgccgc cggggccgcg tggtctgcgg caccaacacg attgcttcgc attacgacta
      481 cctccgggcc gccggcatgt acgacgtctt cgacgcggtc tacgcgtccc acctgatggg
      541 cgtctgtaag ccggatcccg atttctggcg gctcatcctt gaagcggagg gcgtagcggc
      601 cccggacgcg gtcttcgtcg acgatatggc cgagaacgcg gaggcggcgg gcgccctcgg
      661 gcttcgcgcc cacctgttcg acggcgtggc cgggctcgag gcgttcctgg atcggctcga
      721 cgacgagccc gaggcggcag accggaggct cgaggcgtga ctcgccgctc agcgcacggt
      781 cggatcggcc tcgtcgtcgt caccatggcc ggcctagccg tcgcggcgag cgtcgctctc
      841 gcctcgtgcg cccgcgccga tccggtcaag gccgttcccg aggccgacct ggtcgtcttc
      901 tcgtcccacc ctgacgaact cgccaagacc atcatagacg aattcaggga gcgcaccggg
      961 cttcgggtaa ggcttgtatt ggacggcacc ggcgcgctgc tctccaggct atcggctggc
     1021 gagcgggccg acgtgctctg gggcggcggc gccgagagcc tcgtggcgaa cctggggctg
     1081 ttccaaccct acctgtcgcc ggaacgcgac gccgtcccgg ccgcgctcaa ggacgaggac
     1141 ggctactgga ccggcctgac cgtgctaccg atggtcatca tggtcaacgc gcgacttgtg
     1201 ccgcccgagg ccgtaccggc agggtggtca gacctgctgg ctccggagct agcaggcggc
     1261 gtggcgttcg cgaaccccgt ctcttccgcc agctcctata ccatcctgcg cacgatgatg
     1321 gtcgcggcca gggctcgggc ggggggcgac caggacgccg actggcgttt cgtcgagggc
     1381 ttcatcgacg ccatcggcgg ctcgaccctg agcgagtcgg ccatggtata ccgcgtcgtg
     1441 gccgcgggcg agtacctggt cgggctgacc tacgagaacg ccggggccga gtcgatgcgc
     1501 ctcggctcgg acgtcaggat agtctatccg gccgacggca cctcgaccgt gcccgacggc
     1561 gtggccattc tgcgagacgc cgcccacgac gaggcggcca gacgcttcat cgacttcgtc
     1621 ctcggccacg acgtggccag cgtcgtttcg acccgcttca agcggcgttc ggcccgtgtg
     1681 gacgtgccgg caccggacgg tctgccgccg ctcgagagcg tgcggttgct cgactacgac
     1741 ttcgacgctg cggtgactga gcgcgaggcg acgatggcgc gcttcacgac gatgctccag
     1801 gaagccggcc aaaggcgcta gggcgatggg tcccccgtgt cgcggcgtat cctgccgcta
     1861 cggaattcgc tcggggtcat gccgatgacc cgcctgaaca gagtgctgaa atagcgcgcg
     1921 tcacggtacc cggacaagtc cgccacctcc ccgacgcgca cggtaggatc gcgtaagagc
     1981 tccatcgccc tcttcatgcg cagtcgtgtc aggtactcgt tgaaggtcag acccgtggcc
     2041 cgcttgagca gcctggacag gtggtcgccg gagatgccga gccgcccggc gaccgagtca
     2101 gccgacaggt cggtcatgaa gcgctcgcca aggtcggcga tggccgcctc cacgtacgag
     2161 tcgcccgatt cggccgtacg ctcccgggtt aggtcgcgta ggaggccgta ggcctggtcg
     2221 gccgtcgccg cccgctcgag cccggccagc cgttcgcgct ccccggcgcg cttggccgcc
     2281 cggagcatcg cgtcctccag ctcgccatcg tcgacgggct tgatcagata gtcgaacgcg
     2341 ccaagtcgta ccgcttcctt cgcgtagtcg aagtcggcgt gcccggaaac gatgatgaat
     2401 agcggccgtt ccagaggcgc cagttccgcc tcggccttgc gcatcagggc gagcccatcg
     2461 agtcccggca tgcgtatgtc ggtgagcacc acctcggctc ggcgctcgac ggcgaggtcc
     2521 caggcgtgga ggccgtcctc agccgagcct acgagcatgt agccccagcg ttcccagggc
     2581 atcgtcgtct cgagctccct gcgggcgagc tcctcgtcct ccgctatgac gagccgcctc
     2641 atgcgtcctc cttcgcggcg gcggcgagac ccgccggtat cctgaccg
//
LOCUS       DAOOLN010000128         2686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_46634_length_2686_cov_3.103003, whole genome shotgun sequence.
ACCESSION   DAOOLN010000128 DAOOLN010000000
VERSION     DAOOLN010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46634_length_2686_cov_3.103003"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PLW80_02375"
     CDS             complement(<1..324)
                     /locus_tag="PLW80_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPB65376.1"
                     /translation="MSEGRRRSGSGGPTRAAGGSATRATAPGGRPRVAFLYEDDDLVA
                     VDKPCGLATIAPEGSRSKSLYDVVTAHIRRRNPKGRAAVVHRLDRDTSGVIVFAKNAH
                     AKAALM"
     gene            complement(321..605)
                     /locus_tag="PLW80_02380"
     CDS             complement(321..605)
                     /locus_tag="PLW80_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HPB65377.1"
                     /translation="MDDTQGTPTRVEGVEYPVEFEMRVIYLADCRDELTLALERTLTE
                     RGAALERPRELPAKGAKYGRLACRVRFDDQESLYAAYAAIGELAGVKAAL"
     gene            complement(623..2113)
                     /gene="sbcB"
                     /locus_tag="PLW80_02385"
     CDS             complement(623..2113)
                     /gene="sbcB"
                     /locus_tag="PLW80_02385"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008852 - exodeoxyribonuclease I activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HPB65378.1"
                     /translation="MAASILWYDLETFGLDPKHDRIAQFAGLRTNDELEPISDRLVLY
                     ERIAPDYLPSPYSCLVHRISPRRTLEEGIPEYEFAKAIRAEMLAPGTCVAGYNSMRFD
                     DEFVRNLLYRNLFDPYEREWADGNTRWDVLDLFRATRDLRPDGMSWPDGPDGKPDFRL
                     VGLAQANGVPLESAHDAFHDIGATIGLTRLVKRTQPRLYEWYWRHRTRDSLRPLVNLA
                     DREPLVHTAAQYTRPEGCTTIVAPVGMVPGRRDGLVAIDLRFDPSPLVDLSVDEIRRR
                     VFSRKSELADGERIPLVDIRLGRCPYLAPLATMDAGAADRLGLDRGLAIERAGSLARE
                     PELIQKLLAVFAPRAPEPMERDPDYRIYSGGFFRDEDKDAMAAVHEAIATLGPSEARP
                     QAYGMPFIDERLPQLVRRMFARNWPGALSPGEAARWRSFCAGRLLCPRIEGAVDMAGF
                     SKTVESLLGNLDTPAEDKPILLELLEYRRSLEQEVLSYEKEGTSRT"
     gene            2230..2670
                     /locus_tag="PLW80_02390"
     CDS             2230..2670
                     /locus_tag="PLW80_02390"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPB65379.1"
                     /translation="MRDERALLEKYLRDRGLKMTRARETVLDAFLGLESHVSVEAVIE
                     AARRLDPGVGQATVFRTVKLLVDAGLAREACHDDGSRLFEHAYNHAHHDHLTCTRCGA
                     VVEFIDPDIERSQEAVYRRYGFRSSGHRLELYGVCPDCAAKDAE"
BASE COUNT          405 a          891 c          931 g          459 t
ORIGIN      
        1 catcagcgcg gccttggcgt gggcgttctt ggcgaacacg atgacgcccg aggtgtcgcg
       61 gtcgaggcgg tgcaccacgg ccgcccgacc cttggggttg cgcctgcgta tgtgggccgt
      121 gacgacgtcg tagaggctct tggaccggga gccctcgggc gctatggtcg ctagcccgca
      181 gggcttatcg acggctacca ggtcgtcgtc ctcgtagagg aaagcgactc ggggcctgcc
      241 gccaggtgcg gtggctcgcg tcgcggagcc gcccgcggct cgcgtcggcc cgccagagcc
      301 ggacctccgc ctgccttcgc tcacagcgcc gccttgacgc cggccaactc gcctatggcg
      361 gcgtaggcgg cgtacagcga ttcctgatca tcgaaacgga ccctacaggc cagcctgccg
      421 tacttggctc ccttggccgg tagctcgcgc ggccgttcga gggccgcccc ccgctcggtc
      481 agcgtccgtt cgagggctag cgtcagctcg tccctgcaat cggcgaggta gatgacccgc
      541 atctcgaact cgaccggata ctcgacgccc tcgacgcgcg taggcgtccc ctgcgtatcg
      601 tccatagggt tactctcctt gatcatgtcc ggctcgttcc ttccttctcg tacgacagca
      661 cctcctgctc gagggagcgt cggtactcga gcagctcgag caggatcggc ttgtcctcgg
      721 ccggcgtgtc gaggttgccg agtagcgatt cgaccgtctt ggagaagccg gccatgtcga
      781 cggcgccctc gatgcggggg cagagcaggc ggccggcgca gaaggatcgc catcgggccg
      841 cctctccggg cgatagggcg cctggccagt tcctggcgaa catgcgcctg accagctggg
      901 gcaggcgctc gtcgatgaac ggcatgccgt aggcctgggg ccgagcctcg gacgggccaa
      961 gcgtcgctat ggcctcgtgc acggccgcca tcgcgtcctt gtcctcgtcc ctgaagaagc
     1021 cgcccgagta gatgcggtag tcggggtcgc gctccatcgg ctccggcgca cggggcgcga
     1081 acaccgcgag tagcttctgg atcagttccg gttctcgagc caggctcccg gcccgctcga
     1141 tcgccaggcc ccggtccagc ccgagccggt ccgcggcccc ggcgtccatc gtcgccagcg
     1201 gcgccaggta ggggcagcgg cccaggcgga tgtcgacgag ggggatgcgt tcgccgtcgg
     1261 ccagctcgga cttcctggag aagacccggc ggcgtatctc gtcgacggac aggtcgacga
     1321 gcggcgacgg gtcgaagcgg aggtcgatgg cgacgagccc atcccggcgg cccggtacca
     1381 tgccgaccgg cgcgacgatg gtcgtgcagc cctccggcct cgtgtactgg gcggcggtat
     1441 ggacgagggg ctcgcggtcg gccagattga cgaggggccg cagcgagtct cgtgtgcggt
     1501 ggcgccagta ccattcgtac agccgcggct gggtgcgctt gaccagcctc gtcaggccta
     1561 tcgtcgcgcc gatgtcgtgg aacgcgtcat gggccgattc gagcggcacg ccgttggcct
     1621 gggccaggcc gaccagcctg aagtccggct tgccgtcggg gccgtccggc cacgacatgc
     1681 cgtctggacg gaggtctctc gtcgcccgga acaggtcgag tacgtcccag cgcgtgttgc
     1741 cgtcggccca ttcgcgttcg tacgggtcga agaggttgcg gtagagcagg ttgcgcacga
     1801 actcgtcgtc gaagcgcatc gagttatagc cggctacgca ggtgcccggg gcgagcatct
     1861 cggcgcgtat cgccttggcg aactcgtact cgggtatgcc ctcctcgagc gtcctgcgag
     1921 gcgagatgcg gtggacgagg caggagtagg ggctcggcag gtagtccggg gctatgcgct
     1981 cgtagaggac gagcctatcg gatatcggct cgagctcatc gttcgtccgg agcccggcga
     2041 actgcgctat cctgtcgtgc ttggggtcga ggccgaaggt ctcgagatcg taccatagaa
     2101 tgcttgcggc catggggcgg agtataccct agggcgcgcg ggcgagtgaa gcgcgaaata
     2161 aaccttgaca gccctgtctg taaatgataa cccttatcaa tagacttgtt tccgtgaatg
     2221 gagtacgcct tgcgcgatga gagggcccta ctggagaagt acctacggga tcgcggcctc
     2281 aaaatgaccc gagcgcggga gaccgtgctc gacgctttcc tcggccttga atcccacgtc
     2341 agcgtcgagg cggtcatcga agccgcccgg cgcctcgacc cgggtgtcgg ccaggcgacc
     2401 gttttcagga ccgtcaagct gctggtcgac gccgggctgg cccgcgaggc ctgtcacgac
     2461 gacggcagcc gcctgttcga gcacgcgtac aaccacgccc atcacgacca cctgacctgc
     2521 acgcgctgcg gcgccgtcgt ggagttcatc gaccccgata tagagaggtc ccaagaggcc
     2581 gtctataggc gctacggctt ccggtcctcc ggccaccgcc tggagctgta cggcgtctgc
     2641 cccgactgcg ccgccaagga cgccgagtag cgccgagccc ctcctt
//
LOCUS       DAOOLN010000129         2669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_47064_length_2669_cov_2.249426, whole genome shotgun sequence.
ACCESSION   DAOOLN010000129 DAOOLN010000000
VERSION     DAOOLN010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47064_length_2669_cov_2.249426"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PLW80_02395"
     CDS             complement(<1..400)
                     /locus_tag="PLW80_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65380.1"
                     /translation="MDLDRAREYRSVYRVSFYATIVMLIIIPVQILVFVVTKIPATTI
                     AWFELFRDSVVLGMFHADLFILINNVLIAVIYLAFYHSLKDVDKGMLQIGIMIGLIGI
                     AAYISSNKTFELMALSRQYFLAAEGDAKLAI"
     gene            complement(416..1306)
                     /locus_tag="PLW80_02400"
     CDS             complement(416..1306)
                     /locus_tag="PLW80_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPB65381.1"
                     /translation="MHDYRAVRLIRGAKAVVEGAGVRLYRAVGLGDPEECDPFLLLDD
                     FRSDDPRDYSRGFPWHPHRGIETITYVLKGTVEHEDSIGNKGVIGPGDVQWMTAGSGI
                     FHQEMPRGDGDGSMHGFQLWANLPASDKMKEPRYRGIRGEDIPKVTTPNGVVVKIIAG
                     AIDGTTGPIVDVVNLPEYYDVRLPENVEYAHRTTLGSTVLIYVYEGSGSIVGSRASGG
                     SGGLEIGNRSLILFEDGRDIAIKSGAAGLKYLYVSGAPLREPIAWRGPIVMNTREELR
                     LAYEELDNGTFIKTNTRNRH"
     gene            complement(1466..>2669)
                     /locus_tag="PLW80_02405"
     CDS             complement(1466..>2669)
                     /locus_tag="PLW80_02405"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPB65382.1"
                     /translation="EYENVGTHAAVANTILQRDFLREAPLWISSLVSLALAAAVLLVI
                     GRLGTRAQLLVGLGMTVAVALADYAVFHFTGVYVAVLSPLLSTLVSFIAYSLLSFLLS
                     EREKSFLRKAFSTYLSGDVINEIISDPSMLKLGGQKKWITAMFTDIRGFSTISEALDA
                     EQLVKLLNIYLSGMSDIILENRGTIDKYEGDAIISFFGAPLGYQEHARLACRSAVLMK
                     RKEAELNERLLADGMTPNPLLTRIGLNTGDMVVGNMGTERKMDYTIMGNAVNLAARLE
                     GVNKQYGSWILMSDATYQEAGEEFLARRLDRVRVVGINTPVQLWELVGLKADTDEGTL
                     DFLARFEEAHKTFDGRDWKKAARLLSALAAERPDDGPTKSYIKKCEVFTLKPPASDWD
                     GVFSLTEK"
BASE COUNT          510 a          846 c          768 g          545 t
ORIGIN      
        1 cgatcgccag cttggcgtcg ccttctgccg cgaggaagta ctgccgcgat agggccatca
       61 gctcgaaggt cttgttcgag gagatgtagg cggctattcc gatgagcccg atcatgatgc
      121 cgatctgcaa catgcccttg tccacgtcct tgaggctatg gtagaaggcc aggtagatga
      181 ccgctatcaa aacgttgttg atcaggatga agagatccgc gtggaacatc cctagcacga
      241 cgctatctct gaacagttcg aaccaagcta tcgtcgtagc cgggatcttc gtaacgacga
      301 agaccagtat ctgcaccgga ataattatca acatgacgat cgtagcgtag aacgataccc
      361 tataaacgct acgatactcc ctggcccgat ctaaatccat agcgaagctt cctccctagt
      421 gccgattcct cgtgttggtc ttgatgaacg tcccgttatc tagctcctcg tacgcgagcc
      481 gcagctcttc cctggtgttc atgactatcg ggcccctcca cgcgatgggt tcgcggagcg
      541 gcgcaccgga aacgtacagg tacttgagcc cggccgcccc gctcttgatc gcaatatccc
      601 gcccgtcctc gaagaggatg agcgaacggt tccctatctc gagcccaccc gagccgccgc
      661 tcgccctcga gcccacgatg gagcccgagc cctcgtacac gtagataagg acggtactcc
      721 cgagtgtcgt ccgatgcgca tactcgacat tctccggaag gcgtacatcg tagtattccg
      781 gtaagttgac gacatcgacg attggcccgg tcgtaccgtc gatggcgccg gcgataatct
      841 tgacgaccac gccgttagga gtcgttacct tcgggatatc ctcgccgcgt atgcccctgt
      901 acctcggctc tttcatctta tcgctcgccg gcaagttagc ccatagctgg aagccgtgca
      961 tcgagccatc gccgtcaccg cgcggcatct cctgatggaa gatgccgctc cccgcggtca
     1021 tccattggac atcgcctggc ccgatcacgc ccttattgcc gatactgtcc tcgtgctcca
     1081 cggtgccctt gagcacgtac gttatcgtct ctatgcccct atgcgggtgc cacggaaacc
     1141 ccctgctata gtctctcgga tcgtccgagc ggaaatcgtc gagcaagagg aacggatcac
     1201 actcctcagg atcgcccagc ccgacggctc tatagaggcg tactcccgcg ccctcgacga
     1261 ccgccttggc ccctcgaatt aagcgaactg cccgatagtc atgcatgatg tacctcactc
     1321 ctcaacgaag ctgtataagc atcctcgcca cccgccccga aagtatgcgg cgagcgctac
     1381 cgcgcacgac cgcttcaatt tcaggcggct ttcgctgcga gtacgcgatc cggcgccgcc
     1441 agagcgcgag ctcgtcagcg atcgcttatt tttccgtcaa gctgaacacc ccgtcccagt
     1501 cggacgccgg aggcttcagg gtgaagactt cgcacttctt tatatacgac ttcgtcggac
     1561 cgtcgtccgg gcgctcggcg gccagggccg acagcaacct cgcggccttc ttccagtctc
     1621 gcccgtcgaa ggttttgtgc gcttcctcga agcgggccag gaagtcgagc gtcccttcgt
     1681 ccgtatcggc cttgaggccg accagctccc agagctggac cggcgtattg atgccgacca
     1741 ccctgacccg atcaaggcgc ctggccagga actcctctcc ggcctcctgg taggtcgcgt
     1801 ctgacatcag tatccagctg ccgtactgct tgttgacgcc ctcgagcctg gcggcgaggt
     1861 tcaccgcgtt gcccatgata gtgtagtcca tcttgcgctc ggtgcccatg ttgccgacga
     1921 ccatgtcgcc ggtgttcagc ccgatgcggg tcaggagcgg gttgggcgtc atgccgtcgg
     1981 cgagcaggcg ctcgttcagc tccgcttcct tgcgcttcat cagcaccgcg gaacggcagg
     2041 cgagcctggc gtgttcctgg tagccgagcg gggcgccgaa gaacgagatg atcgcgtcgc
     2101 cctcgtactt gtctatagtg ccccggttct cgaggatgat gtcgctcatg ccggacaggt
     2161 agatattgag cagcttgacg agctgctcgg cgtccaaggc ctcggaaatg gtcgagaagc
     2221 cgcggatgtc ggtgaacatc gcggtaatcc attttttctg gccgccgagc ttgagcatcg
     2281 acgggtcgga gatgatctcg ttgatcacgt cgccggacag gtaggtgctg aaagccttgc
     2341 gtaggaagct tttctcccgc tcggaaagga ggaacgatag cagggagtag gcgatgaacg
     2401 acaccagggt ggataggagt ggcgacagga cggcgacgta gacgcccgta aaatggaata
     2461 cggcgtagtc ggccagggcg acggcgaccg tcatgcccaa gccgaccagc agctgcgccc
     2521 gtgtgccgag cctgccgata accagcaata ccgcggccgc gagggcgagc gatactagcg
     2581 acgatatcca cagcggcgcc tcccgtagga agtcgcgctg cagtatcgtg ttggcgaccg
     2641 cggcatgcgt cccgacgttc tcgtattcc
//
LOCUS       DAOOLN010000130         2655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_47395_length_2655_cov_3.074615, whole genome shotgun sequence.
ACCESSION   DAOOLN010000130 DAOOLN010000000
VERSION     DAOOLN010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47395_length_2655_cov_3.074615"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PLW80_02410"
     CDS             complement(<1..220)
                     /locus_tag="PLW80_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65383.1"
                     /translation="MDDNGFCLRPLRASDKAAVMAISAKIWEGDDYLPHSFDDWVSDP
                     DGLFNGCFVGDRLVGFGRVGFVAPGHAWL"
     gene            356..1510
                     /locus_tag="PLW80_02415"
     CDS             356..1510
                     /locus_tag="PLW80_02415"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HPB65384.1"
                     /translation="MNALFALAFLVAPALIVYLCGHFKPLRTIGAVLLCYAAGVVVGN
                     VGVLPEGFAPVQSAMADVSVVLALPLLLFSIDVRAWFKVAGKGMLCMALAALSIIAVT
                     FALQLTFHSGRPDAWQLAGLAVGVYTGGTPNLAAIKAAIGVANDTYILFHTYDAVISF
                     AYIVVMASVARPILLRVARMRGFEPAAGAPETGGDMANDAAGISFREPGAVRGLVLAS
                     LLSAAIVGASAFLGSLAPGTGGTAVTILAVTTLSLAASFIGPVRRVRHTFQAGMYVIY
                     VFCFVVASMTKLDSLVRVDWAVLGYVSASIFGSLVVHALLSKLAGIDVDTFIVTSVSA
                     ICSPPFVPVLAEALKNRAVLMTGLATGLVGYAAGNYLGVSIAYLFRSLPL"
     gene            complement(1491..>2655)
                     /locus_tag="PLW80_02420"
     CDS             complement(1491..>2655)
                     /locus_tag="PLW80_02420"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB65385.1"
                     /translation="ARLGLVVSSEDAAAAEELGDAFDRPRVLRILQATTAAAYVVCAV
                     MGFNAATPFVIAFAMGTLNGTWPIINAIVADAAPAERRKEAFSLLYWGNNIGFSIGPF
                     VAGLLFTRAPRALFIGNGAVLFAAAIIVARYVRGGARTEAPVGVPTIDRQATAAAAAD
                     GPIACPADVQPTAAAAAAPSSVWVVFRRSPVLLLYSAGSVLTAFVYNQHTFALPIFLN
                     DALGEVAGPRVFGAAMMANGLTVVALTALVVLASKRLRSLTSVALASAFYAVGFGAYY
                     LVGGSRYGVPLALGATVVWTVGEILGSTNGNAFVAEKAPPAYRSRVNSVVSFSYIAGG
                     ALAPLAAGSIARAYGSAAVWPFVAASAALSAAFMYGVDRLDKKTGARVTAAGS"
BASE COUNT          410 a          908 c          918 g          419 t
ORIGIN      
        1 cgagccaggc gtggcccggc gcgacgaaac cgacccggcc gaagccgacg agccggtcgc
       61 ctacgaagca gccgttgaac aggccgtcgg ggtcagagac ccagtcgtcg aacgagtgcg
      121 gcaggtagtc gtcgccctcc cagatcttag cgctgatggc catcacggcc gccttgtcgg
      181 aggcgcggag cggcctaagg cagaacccgt tatcgtccat gggccattgg tagcacggct
      241 gggactcggc ggcaaggggt ggaccgctcg acggcggata gccgcgcggc cctcgacccg
      301 gcgcgatagt cgggctacac tgtaccgacc cggcccgacc gacgaggagg aatcgatgaa
      361 cgcgctgttc gccctggcct tcctagtggc ccccgcgctg atcgtctacc tatgcggcca
      421 tttcaagccc ctccgcacga taggcgcggt gctcctatgc tacgcggccg gcgtcgtggt
      481 cggcaacgtc ggagtcctac cggagggttt cgcacccgtc cagtctgcca tggccgacgt
      541 cagcgtcgta ctcgccctgc ccctgctcct gttctccatc gacgtcaggg catggttcaa
      601 ggtggcgggc aagggcatgc tgtgcatggc gcttgccgcc ctgtccataa tcgcggtgac
      661 cttcgcgcta cagctgacgt tccactccgg gaggccggac gcgtggcaac tggccgggct
      721 cgccgtcggc gtgtacacgg gcggaacgcc aaacctggcg gccatcaagg cggccatcgg
      781 cgtcgcaaac gatacctata tccttttcca cacctacgat gcggtcattt cgttcgccta
      841 catcgtcgtg atggcgtcgg tagcgcggcc catcctgctg agggtcgcgc ggatgcgcgg
      901 cttcgagccg gcggcgggag ctccggagac tggcggcgat atggcgaacg acgcggccgg
      961 catctcgttc cgggagcctg gcgcggtccg cgggctggtc ctggcctcgc tcctgtccgc
     1021 ggccatcgtc ggcgcctcgg ccttcctcgg ctcgctcgcg cctggcacgg gcggcaccgc
     1081 ggtaaccatc ctggccgtca ccacgctgtc cctggccgcg tcgtttatcg ggccggtcag
     1141 gcgcgtcagg catacgttcc aggccggcat gtacgtcatc tacgtattct gcttcgtcgt
     1201 cgcgtcgatg acgaagctcg atagcctggt tcgcgtcgac tgggcggtgc tcggctacgt
     1261 gtccgcctcg atcttcggca gcctggtcgt acacgcgctc ctgtccaagc tggccggcat
     1321 cgacgtggat accttcatcg tcacctcggt ctcggctata tgctctccgc cattcgtacc
     1381 ggtactcgcc gaggcgctta agaatcgggc cgtgctgatg actggactgg ccacgggcct
     1441 cgtcggctac gcagccggaa actacctggg cgtatcgatc gcctacctgt tcaggagcct
     1501 gccgctgtga cccgcgcgcc ggtcttcttg tccagccggt cgacgccgta catgaaggcc
     1561 gcggacaggg cggcgctggc cgccacgaaa ggccagacgg ccgcgctgcc gtaggctcgc
     1621 gcgatcgagc cggccgcgag cggcgccaag gcgccgccgg cgatatagga gaacgatacg
     1681 accgagttga cccggctacg gtaggcgggc ggcgccttct cggcgacgaa ggcgttgcca
     1741 ttggtgctgc ccagtatctc gccgaccgtc cagacgacgg tcgcgccgag ggcgagcggc
     1801 acgccgtagc gcgagccgcc gaccaggtaa taggccccga agccgaccgc gtagaacgcc
     1861 gacgccagcg ccacggaagt cagcgagcgt agccgcttcg aagccagtac gacgagcgcc
     1921 gtcagcgcga cgacggtcag gccgttggcc atcatcgcgg ctccgaacac ccttggcccg
     1981 gccacctcgc cgagcgcgtc gttcaggaat atcggtagcg cgaaggtatg ctgattgtag
     2041 acgaaggccg tcagcacgga tccggccgag tacagcagca gcaccgggct acgcctgaac
     2101 acgacccaga cggacgacgg agcggccgcg gcggccgcgg tcggctgcac gtcggccggg
     2161 caggctatcg ggccgtcggc cgcggcggct gcggtggcct gccggtcgat ggtcggaacg
     2221 cccaccggcg cttcggtccg ggcgccgccg cgcacgtagc gggccacgat gatcgcggcc
     2281 gcgaacagca cggcgccgtt gccgatgaac agggcccgcg gcgcccgcgt gaacagcagg
     2341 cccgcgacga agggccctat cgagaagcct atgttgttac cccagtacag gagcgagaag
     2401 gcttccttgc ggcgctcggc cggcgcggcg tcggcgacga tggcgttgat gatgggccag
     2461 gtgccgttga gcgtgcccat cgcgaaggct atgacgaagg gcgtggcggc gttgaagccc
     2521 atgacggcgc agacgacgta ggccgcggcg gtagtcgcct gcaggatccg cagtacgcga
     2581 ggccggtcga aggcgtcgcc tagctcctcg gccgccgcgg cgtcctccga gctgaccacc
     2641 agcccgaggc gggcg
//
LOCUS       DAOOLN010000131         2654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_47415_length_2654_cov_3.701039, whole genome shotgun sequence.
ACCESSION   DAOOLN010000131 DAOOLN010000000
VERSION     DAOOLN010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47415_length_2654_cov_3.701039"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1972)
                     /locus_tag="PLW80_02425"
     CDS             complement(<1..1972)
                     /locus_tag="PLW80_02425"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HPB65386.1"
                     /translation="MDELGLRASFRFEPSADGPEYGVDGLARKLTEARIAGVQGRRLD
                     EIAKTFAVAKAPVTETVASGHAPEAGLPEAAEWAEPSTPDEYLPFAAEALSAAPPPEL
                     YRVRVERVARERIVKKTSALPFLLPKEEKVVDYEKIELREPIAVDTRVIMSFWAPKGA
                     TVAALAPPRPGKPGKSVYGKPIPAPRVEDSAFYLGKGLSRVRNEVKAEVGGFVRVGER
                     WADLIPFGSGDYSVRLSDDGATALLDYTPGDKRLPPPDAAAILEAAHALGLGAETLIG
                     AEETASALLRSTRSGQPLVGFSLSCDRDASARVDVSPDRLRATLTVLKGRGRGKPLSL
                     TMVSEALAGRRLKGVKIDKLKTDVVAFYRGKETELEDYVLAVGKEPTKGKDRTLIYSV
                     AFLPVPQAKEYLEAVEAAPLLSKYAQRLDEFPLSAAGRVALVKKGQEIARFSQPSPGQ
                     AGVDVYGDAIPAPPGNDPLVKAFDYVRFSKESIEGEEDGLVLIGDGEAETASVAEAKP
                     SSGPGDAEPGSEAAAAPKEPPKPSAILVRVLPYRDAKVAVTIAEDAMSAAVDVEKGYG
                     LGRELTLELAQEALAQAGVTEGADLKELAAALNEAREGRIVSSRIIARGAPPVPAGGY
                     RLNWLIRIASGAALTMRTDGSVDYKNQDRSTVV"
     gene            complement(2023..>2654)
                     /locus_tag="PLW80_02430"
     CDS             complement(2023..>2654)
                     /locus_tag="PLW80_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HPB65387.1"
                     /translation="SKARALLESLKKDGASLQRAYIPEILGRVSELDPASRAAFDMRM
                     RKLSPRLVLNMLEDPKDAEKAQKIRRSCREYLGMDLEHLGIVYRDDMQDVALGSRIPI
                     IRYKPQSILSQAIYRLADKLLSSEDDDDAFLDPEYLDDSYGAADTEAEADFDAKLDYV
                     EELLSSGALTQGELIETIRMQQYELGQLRKENQFYKAKMAKALAQGFKA"
BASE COUNT          401 a          880 c          881 g          492 t
ORIGIN      
        1 cgacgacggt agagcgatcc tggttcttat agtcgacgga tccgtcggtc cgcatggtca
       61 aggcggcgcc gctggctatc ctaattagcc agttgaggcg gtagcctccg gccggcaccg
      121 gcggggctcc ccgcgctatg atccgcgacg aaacgattcg gccctcgcgg gcttcgttga
      181 gcgcggcggc cagttccttc aggtcggcgc cctcggtcac gccggcctgc gcgagcgctt
      241 cctgggccag ctccagggtc agctcccggc cgagcccata gcccttctcg acgtcgacgg
      301 ccgcggacat cgcgtcctcg gctatggtca cggcgacctt ggcgtcgcgg tacggcagta
      361 ccctgacgag aatggccgaa ggcttcgggg gctccttcgg agctgcggct gcctccgatc
      421 cgggctcggc gtctccggga ccggaggagg gtttcgcttc ggccacggac gcagtctcgg
      481 cctctccgtc gccgataagg accagcccgt cctcctcacc ctctatcgac tccttggaga
      541 accggacgta gtcgaaggcc ttgaccagcg gatcgttacc gggaggagcc ggaatagcgt
      601 ctccgtatac gtcgacgccg gcctgcccgg gagagggctg gctgaacctg gctatctcct
      661 gtcccttctt gaccagagcg acgcggccgg cggccgagag cgggaattcg tcgagccttt
      721 gggcgtactt ggataggaga ggcgccgcct cgacggcctc gagatactct ttggcctggg
      781 gcaccggcag gaacgcgaca ctatagatca acgtcctgtc tttgcccttg gttggctcct
      841 tgccgacggc caggacgtag tcctcgagtt ccgtctcctt gcctctatag aacgcgacca
      901 catcggtctt gagcttatcg atcttgacgc ccttgaggcg acgcccggcc agagcctcgc
      961 tgaccatcgt cagcgatagc ggtttgcctc ggccccgacc cttgagcacc gtcagcgtgg
     1021 ccctgagcct gtcgggcgaa acgtcgaccc tcgccgaggc gtcgcggtca caggacaagc
     1081 tgaagccgac gaggggctgg ccgctcctcg tcgacctgag cagcgccgag gccgtctcct
     1141 cggcgcctat cagggtttcg gcgcccaggc cgagggcgtg ggcagcctcg agtatcgcgg
     1201 cggcgtcggg aggcggcagg cgcttgtcgc cgggggtata gtcgagcagg gcggtggctc
     1261 cgtcgtccga cagccgcacc gagtagtccc cggagccaaa ggggataagg tcggcccagc
     1321 gctcgccgac ccggacgaaa ccgccaactt cggctttcac ttcgttccta actcgggaaa
     1381 ggcccttgcc gaggtagaag gccgagtcct cgacccgtgg cgccgggatc ggcttgccgt
     1441 agacgctctt gcccggcttg cccgggcggg gcggcgcgag ggcggccacc gtcgctcctt
     1501 taggcgccca gaagctcatg atgaccctcg tgtcgacagc tatcggctcg cgcagctcga
     1561 tcttctcgta gtcgacgacc ttctcctcct tcggcagcag gaaaggtagc gccgacgttt
     1621 ttttgacgat ccgctccctg gctacgcgct cgaccctgac gcggtacagc tccgggggcg
     1681 gcgcggcgga gagggcctcc gccgcgaaag gcaggtactc atcgggcgtg ctcggctccg
     1741 cccactcggc ggcctcgggc aatccggcct cgggcgcgtg tccggaggct acggtctcgg
     1801 tcacgggcgc cttggcaacc gcgaaggttt tagcgatctc gtcgagcctg cggccctgga
     1861 cgccggcgat cctagcctca gtcagcttcc tggccagccc atcgacgccg tactccgggc
     1921 cgtcggccga gggctcgaaa cggaaggaag cccgtagccc gagctcgtcc acgagcaagg
     1981 tcgtctcccc tcgaaaggtc gctccggcca tcgcttcggc tcctaggcct tgaagccctg
     2041 agctagggcc ttcgccatct tggctttgta gaactgattc tccttacgta actggccgag
     2101 ctcgtattgc tgcatacgta tcgtctcgat cagctcgccc tgggtcagcg cgccggacga
     2161 caggagctcc tcgacatagt cgagcttggc gtcgaagtcc gcctcggcct ccgtatcggc
     2221 ggcgccgtag ctgtcgtcga ggtactcggg gtcgaggaaa gcgtcgtcgt cgtcctcgga
     2281 cgagagcaac ttgtctgcca ggcggtagat agcctgcgac agaatggact gcggcttata
     2341 gcggatgatc ggtatgcggc taccgagcgc cacgtcctgc atgtcgtcgc ggtagacgat
     2401 gccgaggtgc tcgagatcca tgccgaggta ttcgcggcag gatcggcgga tcttctgggc
     2461 cttctcggcg tccttcggat cctcgagcat gttgagcacg agcctgggcg acagcttcct
     2521 catacgcatg tcgaaggcgg cgcggctcgc gggatcgagc tcggagacgc gcccgagtat
     2581 ctcggggata taggccctct ggagcgaggc gccgtccttc ttgagcgact cgagcagggc
     2641 gcgggccttg gact
//
LOCUS       DAOOLN010000132         2643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_47700_length_2643_cov_3.740340, whole genome shotgun sequence.
ACCESSION   DAOOLN010000132 DAOOLN010000000
VERSION     DAOOLN010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47700_length_2643_cov_3.740340"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PLW80_02435"
     CDS             complement(<1..187)
                     /locus_tag="PLW80_02435"
                     /inference="COORDINATES: protein motif:HMM:NF015699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65388.1"
                     /translation="MKSMTGYGSARLDSPAIKASIVAKSWNNRFLELAIQMPGYLGPL
                     ERRVRELVERRVARGKLE"
     gene            complement(184..1668)
                     /gene="murC"
                     /locus_tag="PLW80_02440"
     CDS             complement(184..1668)
                     /gene="murC"
                     /locus_tag="PLW80_02440"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPB65389.1"
                     /translation="MSATLPACLAGFRIHLVGAKGTGVCALAELLVGAGAEVTGSDVD
                     DTFYTDEVLAAIGVPVSPFSADNVRGGVDLVIHSKAYTAASNPELAEAEALGVPTMSY
                     AEALGSLSASRDSSGICGVHGKTTTTALAGVMARAARLPTAVLVGSAVGAFGGRSTIT
                     MGNELLIAETCEYRRSFMAFKPRRVVLTSVEPDHQDYYPDYEAIRAAFVEYLVALPPA
                     GTVIYCADDPGAVDAWTRAEAARPDLRGIPYGFSAPGDFGMEGYRVERERGIFKLRGF
                     GLEWRLRVPGRHVALDAAGAVALIAAIVADRDGARRLSEDELSAMAEALESFSGSRRR
                     SEVLGEVGGILFMDDYGHHPTAIRSTLAGLREFYPERRLVVDFMSHTCSRTRALLDEF
                     ASSFADADLLVLHRIYPSAREAPDPETTGRLLYERTVSATALAGRPSRQYYEDPLDAL
                     SFLSGELRPGDLFVTMGAGDNWKLGRALYEAGKAAAEARVKGQP"
     gene            complement(1710..>2643)
                     /locus_tag="PLW80_02445"
     CDS             complement(1710..>2643)
                     /locus_tag="PLW80_02445"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HPB65390.1"
                     /translation="KSAVEDLWKGQCGDAYWMAPTGGIDDPAIREAAYRSLLDAELTT
                     RQKGAFVPGVVKADIDFDGVKEYIYQGNDLNAYLHPRGAVLVELDALKARRNLCDLFS
                     PEPEGDRARKACFVDRLYAAEPGPDAVLRPWAGDIGPFSSHPYEEVLADGNHATLCFA
                     KDGVVSLGGAKRSIAVTKRYSFNKKVVRVAYSVQNRSDSDARFWLGVELNASLGTDRI
                     EWLEASGSDVVSVDPSAMSAARGSGFGAIAKLAIKTVGAVKSIAMATTTPAVVSSALI
                     GSPASQGVATLLLWRIELAADESWKTDILLSVHE"
BASE COUNT          429 a          892 c          894 g          428 t
ORIGIN      
        1 gctccagctt gcccctggcc acgcggcgct ccacgagttc gcgcacccgt cgctcgagcg
       61 gcccgaggta gccgggcatc tggatcgcca gctcgaggaa ccggttattc caggacttgg
      121 cgactatcga cgccttgatg gccggcgaat cgagccgcgc cgaaccgtat ccggtcatgc
      181 tcttcatggt tgtcctttca cgcgcgcttc ggccgcggcc ttcccggcct cgtacagggc
      241 gcggcccagc ttccagttgt cgccggcgcc catcgtgacg aagaggtcgc cgggtcgcag
      301 ctctcctgac aggaacgaca gcgcgtcgag cgggtcttcg tagtactgac ggctcggcct
      361 gccagccaag gcggtggccg agacggtgcg ctcgtagagc aaccggcctg tcgtctccgg
      421 gtcgggagcc tcgcgggccg atggatagat gcgatgcagc acgaggaggt cggcgtcggc
      481 gaacgacgag gcgaactcgt cgagcagggc cctcgtcctc gagcaggtat gcgacatgaa
      541 gtcgacgacg aggcgtcgct ccgggtagaa ttcgcggagg cctgccaggg tggatcgtat
      601 ggcggtcgga tggtggccgt agtcgtccat gaacaggatc ccgccgacct ccccgagtac
      661 ctccgacctg cggcgcgagc cggagaacga ctccagagcc tctgccatgg cggacagctc
      721 gtcctcggaa agccgccgag caccatccct atcggcgacg atggcggcta tcagcgctac
      781 ggcgcccgcg gcgtcgagcg cgacgtgccg gccgggcacc cggagccgcc actcgaggcc
      841 gaagccgcgc aacttgaaga tcccgcgctc acgctccacg cggtagcctt ccatgccgaa
      901 gtcgccgggc gctgagaacc cgtagggtat gcctcgcagg tcgggcctgg cggcctcggc
      961 ccgcgtccag gcatcgacgg ctccggggtc gtcggcgcaa tagatcaccg tcccggcggg
     1021 cggcagggcg accaggtact cgacgaaggc cgcgcggatg gcctcgtagt ccggatagta
     1081 gtcctggtgg tccggctcga cgctcgtcag cacgacgcgg cgcggcttga aggccatgaa
     1141 gctcctgcgg tactcgcagg tctcggctat caagagctcg ttccccatcg tgatcgtcga
     1201 ccggcccccg aaggcgccaa ccgcgctacc aacgagcacg gcggtcggca gccgcgcggc
     1261 gcgggccatc acgccggcga gcgcggtcgt cgtcgtcttg ccgtgtactc cgcagatgcc
     1321 gctcgagtcg cggctcgccg atagcgagcc gagggcctct gcatagctca tcgtcggtac
     1381 gccgagcgcc tcggcctcgg ccagctcggg attggaggcg gcggtatacg ccttcgagtg
     1441 gatgaccagg tcgacgccgc cgcgtacgtt atcggcggag aacggcgaca ccggtacgcc
     1501 gatggcggcc agcacctcgt cggtatagaa cgtatcgtcg acgtccgatc cggttacttc
     1561 cgcgcccgcg ccgaccagca actccgccag cgcgcagacg cccgtgccct tggctccgac
     1621 caagtgtatt ctgaatcccg ccaggcaggc gggcaaggtt gcggacatgg attcatacta
     1681 gctgaaagac gggctctgtg caatcgaggc tattcgtgga cggatagcag tatatcggtc
     1741 ttccagctct cgtcggcggc cagctcgatg cgccagagca atagggtcgc gacgccctgc
     1801 gacgccggag agccgatcag ggccgacgag acgaccgcgg gtgtcgtcgt ggccatcgct
     1861 atggacttga cggcgccgac ggtcttgatg gccaacttgg caatggcgcc gaagccggag
     1921 ccgcgagccg ccgacatggc cgacgggtcg accgatacga catccgagcc cgaggcctcg
     1981 agccactcga tcctgtcggt gccgagcgac gcgttgagct cgacgccgag ccagaagcgc
     2041 gcgtcgctgt cggagcggtt ctggacgcta taggcgaccc gcacgacctt cttattgaac
     2101 gagtagcgct tggtaacggc gatcgagcgc ttggcgccgc ccaggctgac gacgccatcc
     2161 ttggcgaagc acagggtggc gtggttgccg tcggccaaga cctcctcgta ggggtggctc
     2221 gagaagggcc ctatatcgcc ggcccacggc ctcaggacgg cgtccggccc gggctcagcc
     2281 gcatagagcc tgtcgacgaa gcaagccttt ctggcgcggt ccccctcggg ctcgggcgag
     2341 aacaggtcgc ataggttgcg cctggccttg agcgcgtcca actcgacgag cactgccccg
     2401 cgcgggtgca ggtacgcatt gaggtcgttg ccctggtaga tatattcctt gacgccgtcg
     2461 aagtctatat cggccttgac cacgccagga acgaaggcgc ctttctgccg cgtcgtcagc
     2521 tcggcgtcga gcagtgaccg gtaggccgcc tcgcgtatcg ccggatcgtc gatgccgccg
     2581 gtcggcgcca tccaatacgc gtcgccgcac tggcccttcc agagatcctc gacggcgctc
     2641 ttc
//
LOCUS       DAOOLN010000133         2643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_47710_length_2643_cov_1.926198, whole genome shotgun sequence.
ACCESSION   DAOOLN010000133 DAOOLN010000000
VERSION     DAOOLN010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47710_length_2643_cov_1.926198"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLW80_02450"
     CDS             complement(<1..258)
                     /locus_tag="PLW80_02450"
                     /inference="COORDINATES: protein motif:HMM:NF039826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HPB65391.1"
                     /translation="MFGFVSANPERLSPEAAERYQAGYCGLCHALGSRHGAAARLTVN
                     YDMTFLAVYLSAIHGEPGERGTERCVVHPLKAHEYLGGRIAD"
     gene            complement(243..1955)
                     /gene="dnaK"
                     /locus_tag="PLW80_02455"
     CDS             complement(243..1955)
                     /gene="dnaK"
                     /locus_tag="PLW80_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPB65392.1"
                     /translation="MGKAIGIDLGTTNSCVAIMEGGDAVVLPNAEGGRTTPSVVAFTA
                     AGERLVGQTARRQAVINHERTVSSIKRDMGSDRRVEIDGRRYSPQEISAMILQKLKAD
                     AEAYLGETVREAVITVPAYFTDAQRQATKDAGRIAGLEVKRIINEPTAAALAYGVDKD
                     YGQKVMVYDLGGGTFDVSLLSIGDGLIEVLATSGNNRLGGDDFDACLVDHLVAGFRKE
                     HGVDLAKDPAAMQRLREAAEKAKIELSGLVSTAVSLPYLTVGRSGPLHLEQTVARADF
                     DRLTAHLVEATMEPMRQAMRDSGLERGDIDRILLVGGSSRIPAVQAAIKAFMGKDPSK
                     GVNPDECVAIGAALQAGVLQGEVKDLLLLDVTPLSLGIETMGGVFSRIIDRNTSIPIK
                     KSQVFTTAANFQTSVDVHVLQGEREMAKHNKTLGKFKLSGIRRAPRGAPQIEVSFDID
                     ANGIVNVSARDLGTGKAQAITIEASSSLSDEEIERAIRDAKRYQAEDARQKSEVSAKD
                     DAERLIGQASSMLKGLDRDDKAAVEELIKAAKKAVKSKDAAQILAANEALRAAIEERT
                     CSDS"
     gene            2113..>2643
                     /locus_tag="PLW80_02460"
     CDS             2113..>2643
                     /locus_tag="PLW80_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB65393.1"
                     /translation="MFSILVVEDDDNARRLMETVLRRGGYEAIIAADGAEALERMRER
                     HVDLAVVDLMMPRMDGYEFTEALRRYRDETPVLMVTAMEGRADKRRGFVSGADDYMVK
                     PVDEEELLLRIKALLRRARIASERRLGIGGTTLDYDSMTVTRGGRVAELPAKEFALLE
                     LLLRRQGEVLPRSLIAS"
BASE COUNT          384 a          835 c          946 g          478 t
ORIGIN      
        1 gtccgctatg cgcccgccca ggtactcgtg ggccttgagc gggtggacga cgcaccgctc
       61 cgttccgcgc tcgcccggct cgccgtggat ggccgatagg tagacggcca ggaaggtcat
      121 gtcgtagttg accgtcagcc tggcggccgc gccgtgcctg gaaccgagcg cgtggcataa
      181 cccgcagtag ccggcctggt agcgctcggc ggcctcgggc gacaggcgct cggggttggc
      241 gcttacgaat ccgaacacgt gcgctcctcg atggccgcgc gcagggcctc gttggcggcc
      301 aggatctggg ccgcgtcctt cgacttgacg gctttcttgg cggctttaat gagttcctcg
      361 acggcggcct tatcatcgcg atcgagtccc ttgagcatgg aggaagcctg gcctatcagg
      421 cgctcggcgt cgtccttggc cgatacctcg ctcttctgcc tggcgtcctc ggcctggtag
      481 cgcttggcgt cgcggatggc gcgctcgatc tcctcgtcgc taagggagga gctagcctcg
      541 atggtgatgg cttgggcctt gccggtgccg aggtcgcgcg ccgagacgtt gacgatgccg
      601 ttggcgtcga tatcgaagct gacctcgatc tgaggcgcgc cgcgcggcgc ccgccggatg
      661 ccggatagct taaacttgcc gagggtcttg ttgtgctttg ccatctcgcg ctcgccctgg
      721 agcacgtgca cgtcgaccga ggtctggaag ttcgccgccg tggtgaagac ctggctcttc
      781 ttgatcggta tcgaggtgtt gcggtcgatg atcctggaga acacgccgcc catcgtctcg
      841 atgccgaggg acagcggcgt cacgtcgagg aggaggaggt ccttgacctc tccctggagc
      901 acgccggcct ggagcgcggc gccgatggcg acgcattcgt cgggattgac gcccttggac
      961 ggatccttgc ccatgaaggc cttgatcgcg gcctgcacgg ccggtatgcg cgacgagccg
     1021 ccgacgagca ggatgcggtc gatgtcgccg cgctcgaggc cggagtcgcg catggcctgg
     1081 cgcatcggtt ccatggtcgc ctcgacgagg tgggcggtca gccggtcgaa gtcggccctg
     1141 gcgaccgtct gctcgaggtg caggggcccg gagcggccga cggtcaggta gggcaggctg
     1201 accgcggtcg agacgaggcc gctcagctcg atcttggcct tctcggcggc ctcgcgcaga
     1261 cgctgcatcg cggccgggtc cttggcaagg tcgacgccgt gctccttcct gaagccggcg
     1321 acgaggtggt cgacgaggca ggcgtcgaag tcgtcgccgc cgaggcggtt gttgccggag
     1381 gtcgccagca cctcgatcag gccgtcgcct atggacagga gcgacacgtc gaaggtgccg
     1441 ccgccgaggt cgtagaccat caccttctgg ccgtaatcct tgtcgacgcc gtaggcgagg
     1501 gcggcggcgg tcggctcgtt gatgatgcgc tttacctcga gcccggcgat gcggccggcg
     1561 tccttggtcg cctggcgctg ggcgtcggtg aagtaggccg gcacggtgat caccgcctcg
     1621 cggaccgtct cgcccaggta ggcctcggcg tcggccttga gcttctggag tatcatcgcc
     1681 gatatctcct ggggagagta ccgccgcccg tcgatctcga cgcggcggtc gcttcccatg
     1741 tcccgcttga tcgacgagac ggtgcgctcg tggttgatca ccgcctgccg cctggccgtc
     1801 tggccgacga ggcgctcccc cgcggcggtg aacgcgacca ccgacggcgt cgtgcgcccg
     1861 ccctccgcgt tcggcagcac gacggcgtcg ccgccctcca tgatagccac gcacgaattg
     1921 gtcgtgccca ggtctatgcc tatagccttg cccatgtact ctcctcgggg cgccagccgg
     1981 cgccgttacg tggataggat agcgacccaa gatgaactga atgtaaacgg cttggcgaat
     2041 cgccccggaa taacgactat gtagtagtgg tacgaagctt gttggtcgta tagtaggatg
     2101 gctcggaggg atatgttcag cattcttgtg gtcgaggacg acgataacgc gaggcgcctc
     2161 atggagaccg tgctacgccg cggcggctac gaggcgataa tcgcggccga cggcgccgag
     2221 gcgctcgagc gcatgcggga gcgccacgtc gacctggccg tcgtcgacct gatgatgccg
     2281 cggatggacg gctacgagtt caccgaggcg ctcaggcgct atcgcgacga gacgccggtg
     2341 ctgatggtca ccgcgatgga gggccgggcc gataagcggc gcggcttcgt gtccggcgcg
     2401 gacgactaca tggtcaagcc ggtggacgag gaggagctgc tcctcaggat caaggcgctg
     2461 ctcaggcggg cgcgcatcgc ctccgagcgc aggctcggga taggcgggac gacgctcgac
     2521 tacgactcta tgaccgtgac gaggggcgga cgggttgcgg agctgccggc caaggagttc
     2581 gcgctgctcg aactgctgtt gcgccgccag ggcgaggtgt tgccgagatc gctgatcgcc
     2641 tcc
//
LOCUS       DAOOLN010000134         2621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_48242_length_2621_cov_3.871785, whole genome shotgun sequence.
ACCESSION   DAOOLN010000134 DAOOLN010000000
VERSION     DAOOLN010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48242_length_2621_cov_3.871785"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="PLW80_02465"
     CDS             <1..908
                     /locus_tag="PLW80_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase cyclase
                     subunit"
                     /protein_id="HPB65394.1"
                     /translation="GAWGQALLERWLVAGGAPANAPTESSSITAEPSMAEPGTVPRRG
                     TLPRIIPCLDVVGGRVVKGVRFEELRDSGDPAELAARYEASGADEIVLLDISAGLEGR
                     ETAAETVRAVRRRIGIPLCVGGGVRSLADAERVLAAGADKVALNSRGYRDPGLLTAIA
                     REFGRQCCVAAVDATRRGRSSGVAPDGAPVVVLDAGRTETATPVTDWIRRAVDAGAGE
                     LLLTSRDRDGTRSGYDLELLHSACAAAPGTPVIASGGAASPEQILEGLQAGADAALLA
                     GALHDGSLTIAAIKDYMRREGAEVR"
     gene            908..2263
                     /gene="hisE"
                     /locus_tag="PLW80_02470"
     CDS             908..2263
                     /gene="hisE"
                     /locus_tag="PLW80_02470"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HPB65395.1"
                     /translation="MLIPSIDILGGRAVQLVGGERFELDCGDPLELAERFSVLGELAV
                     VDLDAAMGSGSNLELVAELASRWPCRVGGGVRDESTMRRLLDSGARSVMVGTRATPEF
                     LSAFPRERLIAALDFKRGVVMTEGWKTSTGASVADKAAVLAPYVGGIIVTAIEIEGSL
                     SGIDLNASRALVESIRAAGFDGVLTLAGGVSSAAEVATLDAMGVDAQVGMALYKGVLD
                     PADAFAACLSSDRPDGLWPTVVCDESGVALGLAYSDAESLKAAITERSGVYRSRRRGL
                     WRKGESSGDRQTLLRVDADCDRDALRFTVRQSGAGFCHRGTRTCFGAVGAAGFGGDDR
                     GFALVSRTTERRLAEVAAGEAPAGSYTARLFTEPGLLDAKIAEEAAELCEARGPGRSA
                     EEAADLVYFVSVKLASDGTSLADVERVLDRRALRVTRRPGNAKSPYREQGNEAWKTGI
                     H"
     gene            2242..>2621
                     /locus_tag="PLW80_02475"
     CDS             2242..>2621
                     /locus_tag="PLW80_02475"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPB65396.1"
                     /translation="MEDRYTLRRVGLEEIGRRGRPADDPACEATARRIMDEVRDGGEA
                     ALARLAAELDGNAGRLWYGPDDFASALQSIPAAERGVLERAAGRVGAFAAAQLASLGT
                     VDLAVPGGRAGHEVPALVAGLRQL"
BASE COUNT          376 a          873 c          992 g          380 t
ORIGIN      
        1 ccggggcttg gggccaggcc ctgctcgagc gctggctggt cgcgggaggc gctccggcga
       61 acgctccgac cgagtcgtca tcgatcacgg cggaaccgag tatggcggag cccggcacgg
      121 taccgaggcg gggaaccctg ccgaggatca ttccatgcct cgacgtggtc gggggccgcg
      181 tcgtcaaggg cgtacgcttc gaggagctgc gcgactcggg cgacccagcg gagctggcgg
      241 cgcgctacga ggcgtcggga gccgacgaga tcgtcctgct cgacataagc gcgggcctcg
      301 aagggcgcga aaccgcggcg gagaccgtgc gggccgtcag gcggaggata ggcatacccc
      361 tgtgcgtcgg aggcggcgtc aggagtctcg ccgacgctga gcgcgtgctc gccgccggcg
      421 ccgacaaggt cgccctcaac tccaggggct atcgcgaccc gggtctcttg acggcgatcg
      481 cgcgcgagtt cggccgccag tgctgcgtcg ccgcggttga tgcgacgagg cgcggccggt
      541 cgtccggcgt cgcgcctgac ggcgcgcccg tcgtcgtgct ggacgcgggc cggacagaga
      601 cggctacccc ggtaacggac tggatacgcc gggccgtcga cgccggggcc ggcgagcttc
      661 ttttgacgag ccgtgaccga gacggcaccc gctcaggcta cgacctcgag ctcctgcatt
      721 cggcctgcgc cgccgcgccc ggaaccccgg tgatagcgtc gggcggagcg gccagcccgg
      781 agcagatact ggaaggccta caggccggag ccgacgcggc gctgctcgcc ggcgccctgc
      841 acgacggctc gttgacaatt gccgcgatca aggactacat gcgccgcgag ggcgcggagg
      901 tacgctgatg ctgattccat cgatcgatat cctgggcggc agggccgtcc agctcgtcgg
      961 gggcgagcgt ttcgagctgg actgcgggga tccgctcgag ctcgcggagc gcttctccgt
     1021 actcggtgag ctggccgtcg tcgacctgga cgccgccatg ggctccggct ccaaccttga
     1081 gctcgtggcg gagctggctt ctcgctggcc gtgcagagtc ggcgggggcg tgcgcgatga
     1141 gtcgacgatg cgccggctcc tcgactcagg cgcccgctcc gtcatggttg gtaccagggc
     1201 gacgccggaa ttcttgtccg ctttccccag ggaacgtctc atagccgccc tcgacttcaa
     1261 gcgcggcgtc gtcatgaccg agggctggaa gaccagcacc ggagcgagcg tagccgataa
     1321 agccgccgtg ctggctccgt acgtcggcgg tatcatcgtc accgctatcg agatagaggg
     1381 ctcgctgtcg ggtatcgacc tgaacgcgtc ccgcgcgctt gtcgagtcga tacgcgcggc
     1441 cggcttcgac ggggtgctga cgctcgccgg tggagtgagc tcggccgccg aggtggctac
     1501 cctcgacgcg atgggcgtcg acgcgcaggt cggcatggcc ctatacaagg gcgtactcga
     1561 tcccgcggac gcgttcgccg cgtgcctgtc ttccgaccgg cccgacgggc tgtggcccac
     1621 ggtcgtctgc gacgagtcag gcgtcgcgct cgggctcgcg tactcggacg cggagagcct
     1681 taaggctgcc ataacggagc gctccggcgt ctatcgctcg cgtaggcgtg ggctgtggcg
     1741 caagggcgag tcgagcggcg accgccagac tctcctacga gtcgacgccg actgcgaccg
     1801 agacgcgcta cgttttaccg tgcgccagtc tggcgccggg ttctgccacc ggggaacgcg
     1861 gacctgcttc ggggccgtcg gggcggccgg gttcggcggc gacgatcgcg gcttcgctct
     1921 ggtatcgagg acgacggagc ggcgcctcgc cgaagtcgcc gcgggcgaag cgccggcggg
     1981 ctcatatacg gccagactct tcacggaacc gggcttgctc gacgccaaga tagccgagga
     2041 ggccgccgag ctatgcgagg ctaggggacc ggggcgctcc gccgaggagg cggccgacct
     2101 cgtctatttc gtatcggtga agctggccag cgatgggacg agcctcgcgg acgtcgagcg
     2161 cgtgctcgac cgccgggctc tgcgcgtgac caggcggccc ggtaacgcca agtcgccgta
     2221 tagggagcag gggaacgaag cgtggaagac cggtatacac tgaggcgcgt cggcctcgag
     2281 gagataggcc gtaggggccg accggccgac gacccggcct gcgaggcgac ggctaggcgc
     2341 atcatggacg aggtacggga cggcggcgag gcagcgctcg cccgcctggc agccgagctc
     2401 gacggcaacg ccggccggct gtggtacggc ccggacgact tcgcctcggc gctgcaatcg
     2461 atcccggccg ccgagcgcgg ggtcctggaa cgggcggccg gcagggtagg ggccttcgcc
     2521 gccgcccaac tcgcctcgct cggaaccgtg gacctcgcgg tacccggcgg ccgggccggc
     2581 cacgaggtgc cggcgctggt ggccgggctg cgccagttgg a
//
LOCUS       DAOOLN010000135         2607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_48604_length_2607_cov_2.602273, whole genome shotgun sequence.
ACCESSION   DAOOLN010000135 DAOOLN010000000
VERSION     DAOOLN010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48604_length_2607_cov_2.602273"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1533)
                     /locus_tag="PLW80_02480"
     CDS             complement(<1..1533)
                     /locus_tag="PLW80_02480"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPB65397.1"
                     /translation="MAIATTKIRIGQSIGTRIALLFGLSTLAVLSALALIIAQQAFTS
                     AEDLSEAYSVQITDARADELAAIVSKYVRFAQDIAIRDVIIGGDYAEVTNALKSMNRV
                     IEQEVAGGFVAWPSGDYVTALGVPGNIADRDYFQDIMADKADIRVSNPVISKALGIPI
                     VVVAAAIKRNGRNDGMVGLQIPLDNLSRQIADVKLSDNSVAILVDGDGMVIAHPDPKI
                     VMNLNILKADEIGYEGLSAAGRIMQRGEPVSMRMKRPDGAMLHGFASPIANTPNWSLA
                     VLVPLSEIQASGRSIAVLVVAFSVAAIAIIVALSFLFGRSIAKPVRSIADAANELADG
                     KLDIEIPESAIRRVDEIGALSRSMGSTIARLREVVAEVAETSDSVSSGATELSTSAQQ
                     MAEGIAGIANSSQQLSQGATEQAASAEEVSASVEQMGANIKQNADNSSQTEKIATKAA
                     GDARQGAEAVAQTVQAMRQIAEKIGIIEEIARSTNMLSLNASIEAARAGEHGKGFAVV
                     ASE"
     gene            complement(1626..>2607)
                     /locus_tag="PLW80_02485"
     CDS             complement(1626..>2607)
                     /locus_tag="PLW80_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPB65398.1"
                     /translation="GFQIVVEHVRPGLDDDFDGRMAAGHDEAGMLVSSLAATVAKLRD
                     VITNVQGASAMMIDGSAKLSDASKQMAAGIAGIAESSQQLSQGSSEQAASAEQVSASV
                     EQMSANIKQNADNSFQTERISSKAATDAREGLTAVKDTVAAMRQIAERIAIIEEIASQ
                     TNMLSLNASIEAARAGEHGKRFAVVASEVGKLAERSKLAAGEISVLSQRSVEIADKAG
                     ALLEDMAPDIQKTADLVQEISVASREQDTGAQQINLAMTQLDSVIQQNASISEEFSAT
                     SEEIAGQARLVADTSTELADQAIGCRRWLGSSRSMARCDALSRNSNIIIL"
BASE COUNT          383 a          857 c          827 g          540 t
ORIGIN      
        1 ctcgctcgcc accaccgcga agcccttgcc gtgctcgccg gcccgcgccg cctcgatgct
       61 cgcgttcagg gacagcatgt tcgtcgatcg cgcgatctcc tcgatgatgc ctatcttctc
      121 ggctatctgg cgcatcgcct gcacggtctg ggcgacggcc tcggctccct gcctggcgtc
      181 gccggcggcc ttggtggcta tcttctcggt ctgggacgag ttgtcggcgt tctgcttgat
      241 gttggcgccc atctgctcga cggacgcgga gacctcctcg gcgctcgcgg cctgctcggt
      301 cgcgccctgg gacagctgct ggctcgagtt ggctatgccg gctatgccct cggccatctg
      361 ctgggccgag gtcgacagct ccgtggcgcc ggaggagacc gagtcgctcg tctcggcaac
      421 ctcggcgacc acctcgcgca ggcgggcgat ggtcgagccc atcgatcgcg acagcgcgcc
      481 gatctcgtct acgcggcgga tagccgactc gggtatctct atgtccagct tgccgtcggc
      541 cagctcgttg gccgcgtcgg cgatggaacg taccggcttg gctatggagc ggccgaacag
      601 gaacgacagc gcgacgatga tggctatggc cgccacgctg aaggccacga cgaggacggc
      661 gatggatcgt ccgctcgcct ggatctcgct gagcggcacg agcacggcca gcgaccagtt
      721 cggggtattc gcgatcgggg acgcgaagcc gtgcagcatc gcgccgtcgg gccgcttcat
      781 gcgcatcgat accggttcgc cgcgctgcat gatccgtccg gcggcggaca ggccctcgta
      841 gcctatctcg tcggccttga ggatgttcag gttcatgact atcttcgggt ccgggtgcgc
      901 gatgaccatg ccgtccccgt cgaccagtat ggcgacgctg ttgtcgctca gcttgacgtc
      961 ggctatctgc ctggacaggt tgtcgagcgg tatctggagg ccgaccatgc cgtcgttgcg
     1021 gccgttccgc ttgatggctg ccgcgacgac gacgatcggt atgccgagcg cctttgatat
     1081 caccggattg gagacgcgta tgtccgcctt gtctgccatg atgtcctgga agtagtcgcg
     1141 gtcggctatg ttgcccggca cgccgagcgc cgtgacgtag tcgccgctcg gccatgccac
     1201 gaacccgccg gctacctcct gctcgatcac ccggttcatg ctcttgagcg cgttcgtcac
     1261 ctcggcgtag tcgccgccta tgatcacgtc gcgtatcgcg atgtcctggg cgaagcggac
     1321 gtatttcgag acgatggcgg ccagttcatc ggccctggcg tcggttatct gcaccgagta
     1381 ggcctcggat agatcctcgg cgctcgtgaa cgcctgttgc gcgatgatga gcgcgagggc
     1441 ggacaggacg gcgagcgttg ataatccgaa cagcaacgct atgcgggttc cgatggattg
     1501 gccgatacgt atcttcgtcg tcgcgattgc catggtcgcc ctcctcgatc gtccaatgat
     1561 catccagcac gataattctt acgtacccgt tcgtagaatg caaccaaacg gtgctccgcg
     1621 agattctata aaataatgat attagaattc ctcgataaag cgtcacacct tgccatggat
     1681 cgagaagaac ccaaccaacg cctgcagcct atggcctggt cggcgagctc cgtggaggta
     1741 tcggcgacta gccgagcttg cccggctatc tcctcgctcg tcgcgctgaa ctcctccgag
     1801 atggacgcgt tctgctggat gaccgagtcg agctgggtca tcgccaggtt gatctgctgc
     1861 gccccggtgt cctgctcgcg gcttgccacg ctgatttcct ggaccaggtc ggccgtcttc
     1921 tggatatcgg gggccatgtc ctcgagcagc gcgcccgcct tgtcggctat ctcgacgcta
     1981 cgctgcgaaa gcaccgatat ctccccggcg gctagcttgg agcgctcggc cagcttgccc
     2041 acttcgctcg ccacgaccgc gaaacgcttg ccgtgctcgc ccgcgcgagc cgcctcgatg
     2101 ctggcgttca gggacagcat gttggtctgg ctggcgatct cctcgatgat ggcgatgcgc
     2161 tcggcgatct ggcgcatcgc ggccaccgta tccttgacgg ccgtcaggcc ttctctcgcg
     2221 tcggtggccg ccttggacga gatgcgctcg gtctggaacg agttgtcggc gttctgcttg
     2281 atgttggcgc tcatctgctc gacgctggcg gacacctgct cggcgctcgc cgcctgctcc
     2341 gacgagccct gggacagctg ttggctcgat tcggctatgc cggctatgcc cgcggccatc
     2401 tgcttcgacg cgtccgacag cttggccgag ccgtcgatca tcatggcgct cgccccctgg
     2461 acgttcgtga tgacgtcgcg cagcttggcc accgtcgcgg ccagggacga tacgagcatg
     2521 cccgcctcgt catggccagc ggccattctg ccgtcgaagt cgtcgtcgag gccgggccgg
     2581 acgtgttcaa ccacaatctg gaaaccg
//
LOCUS       DAOOLN010000136         2598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_48848_length_2598_cov_2.892253, whole genome shotgun sequence.
ACCESSION   DAOOLN010000136 DAOOLN010000000
VERSION     DAOOLN010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48848_length_2598_cov_2.892253"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PLW80_02490"
     CDS             <1..750
                     /locus_tag="PLW80_02490"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPB65399.1"
                     /translation="AVVVFADNAVQLDLDPAALARIGFDPGNRYSVPFMMAAAGVSVN
                     RTKVSGYEHSWSVFDRSDLGGRMTMLDDMREVLGAALKSLGYSVNDPDPAHIEEARRV
                     VERWKPNLVKFDAESFAKSFASGEFWVVQGYAENVFIEYPEARRAEVDFFFPKEGMPM
                     YMDSFCVLRGAKNVERAYAFIDYMLRPDVAARVADYLMLPSPNVPARALMKVEPNYDF
                     DDLARGEFKEDLGREALKLYNEAWRRIRVGN"
     gene            complement(889..>2598)
                     /locus_tag="PLW80_02495"
     CDS             complement(889..>2598)
                     /locus_tag="PLW80_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HPB65400.1"
                     /translation="RRVVAAVYLSEGDSGAAVLLFLPLRSSLNDEVRLAIERAARALS
                     GEDQPPSIAVDYPGYAAIRFDGVAIPAYGSGSTMRLGSLKAQAPSSLAVWVDPSAIAT
                     LLESMPGGLGAMFGGSSDYDDYDDYDDYDDYDDYDDYDYDDDGIDNAGIDNAGIDNTG
                     IDNTGIDNTGIDNAGDGAEDYAEDYAWDEPGQVDTLGAYDDLAALGANGPLDDVPGAI
                     GEALKAGLEELTGLSLALTVQPDRAWLRFGVELAAGGTLAELAEAASSGDRSLPYLSY
                     CDADALISFAWSAPYDWSLPLLEGLYALILPDGSFADDALAAAKAYAAASGLNGGMSI
                     SLAPSAELVRAVRTNADLDDAEALELVSRGLGLSVSGAMELRDRQAFRDAAAEAFGAI
                     AAPEYAELMEASGLSMSVDRGVGVASGMPYDAYRYEFTPTEAEEADSGEAMAMRLIGR
                     LMAPVMVYRDDKAFIGLGDPKAVASGIAKDGAASPLRADKAFKALRAGASADARALTY
                     VSTRNLARLILRLMPDDQEPLGFNAANLSGLLMWLDATPATLGFGLGLGAEDVKAAVA
                     IAN"
BASE COUNT          413 a          889 c          856 g          440 t
ORIGIN      
        1 gcggtggttg ttttcgccga caatgccgtg caactggacc tggacccggc cgccctggcg
       61 cgcatcggct tcgacccggg caaccggtac agcgtcccgt tcatgatggc cgcggccggc
      121 gtctcggtga accggaccaa ggtctccggc tacgagcata gctggagcgt cttcgaccga
      181 tcggacctcg gcggccgcat gaccatgctc gacgacatgc gcgaggtgct cggagcggcg
      241 ctcaagtccc tgggctactc ggtcaacgac cccgacccgg cccatatcga ggaggcccgc
      301 cgcgtcgtcg agcgctggaa gccgaacctg gtcaagttcg acgccgagtc cttcgccaag
      361 agcttcgcct ccggcgagtt ctgggtagtg cagggctacg ccgagaacgt gttcatcgag
      421 tacccggagg ccaggcgcgc cgaggtcgac ttcttcttcc cgaaggaggg catgccgatg
      481 tacatggaca gcttctgcgt gcttaggggc gccaagaacg tcgagcgggc ctacgcgttc
      541 atcgactaca tgctcaggcc cgacgtcgcc gcgcgcgtcg ccgactatct gatgctgccc
      601 tcgccgaacg tgccggcccg cgccctgatg aaagtcgagc cgaactacga cttcgacgac
      661 ctggcccgcg gcgagttcaa ggaagacctc ggccgcgagg cgctcaagct atacaacgag
      721 gcctggcgcc gtatccgcgt aggcaactga tcgtcgttca agccaggcat agagaactgg
      781 aggatacgag atacgcgaga ggagagagag ggggattcgc tgatctactc tctctcctcc
      841 tcttgcccga ctcttggact cgtatcctct tcgtccatct tcgcggactc agttcgctat
      901 cgcgacggcg gccttgacgt cctcggcgcc gagtcccagg ccgaagccga gcgtggcggg
      961 cgtcgcgtcc aaccacatga gcaggccgga taggttggcc gcgttgaagc cgagcggctc
     1021 ctggtcgtcc ggcatcaggc gcagtatcag cctagccaga ttcctcgtcg atacgtaggt
     1081 cagggcccgc gcgtcggccg aggctcccgc cctgagcgcc ttgaacgcct tgtccgcgcg
     1141 tagcgggctc gcggcgccgt ctttggctat cccggaggcc acggccttgg gatcccccag
     1201 gccgatgaag gccttgtcat cgcgatagac catgaccggg gccatcagcc tgccgatcag
     1261 cctcatcgcc atggcctcgc ccgaatcggc ctcctcagcc tccgtcgggg tgaactcgta
     1321 gcggtaggcg tcgtagggca tgcccgaggc cacgccgacg ccgcggtcca ccgacatcga
     1381 aagcccggac gcttccatca gttccgcgta ctcgggcgcg gctatggcgc cgaaggcctc
     1441 ggccgcggcg tcgcggaaag cctgcctgtc gcgtagctcc atcgccccgg agaccgacag
     1501 cccgaggccg cgcgacacca gctcgagcgc ctcggcgtcg tccaggtcgg cgttcgtccg
     1561 gacagcccga accagttcgg ccgacggtgc cagggatatc gacatcccgc cattcaggcc
     1621 gctcgccgcg gcgtaggcct tggccgccgc gagcgcgtcg tcggcgaacg agccgtcggg
     1681 caggatcagc gcgtagagcc cctcgagcag cggcaggctc caatcgtacg gggccgacca
     1741 cgcgaaggat atgagcgcat cggcgtcgca gtagctcagg tagggtaggg agcggtcgcc
     1801 cgaggacgcg gcctcggcta gctcggccag cgttccgccg gcggccagct ccacgccgaa
     1861 gcggagccag gcccggtcgg gctggacggt caacgcaagc gagagtccgg tcagctcttc
     1921 caggcccgcc ttcagcgcct cgcctatcgc ccccggcacg tcgtcgagcg ggccgttagc
     1981 gccgagcgcc gccaggtcgt cgtacgctcc tagagtgtcg acctggcccg gctcgtccca
     2041 cgcgtagtcc tcggcgtagt cctcggcccc gtcgcctgcg ttgtcgatgc ccgtgttgtc
     2101 gatgcccgtg ttgtcgattc ccgtgttgtc gatgcctgcg ttgtcgatgc ctgcgttgtc
     2161 gatgccgtcg tcgtcgtaat cgtagtcgtc gtagtcgtcg taatcgtcgt aatcgtcgta
     2221 atcgtcataa tcgtcgtaat cggacgatcc cccgaacatg gcgccgagcc cgccgggcat
     2281 cgactcgagc agggtggcta tggccgacgg gtcgacccag acggccaggc tggacggcgc
     2341 ctgcgccttg agcgacccga gcctcatcgt cgatccggag ccataggcgg gaatagcgac
     2401 gccgtcgaag cggatcgcgg catagcccgg gtagtcgacg gcgatgctcg gaggctggtc
     2461 ttctccgctc agggccctcg cggcccgttc gatcgcgagg cgaacctcgt cgtttaggga
     2521 cgatcgtagc ggcaggaaga gtagcaccgc ggccccgcta tcgccttcgg atagatagac
     2581 ggccgccacg acgcgcct
//
LOCUS       DAOOLN010000137         2597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_48883_length_2597_cov_2.439811, whole genome shotgun sequence.
ACCESSION   DAOOLN010000137 DAOOLN010000000
VERSION     DAOOLN010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48883_length_2597_cov_2.439811"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="PLW80_02500"
     CDS             complement(<1..888)
                     /locus_tag="PLW80_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65401.1"
                     /translation="MTINDFIALDWAETEGPLIEPPSFSPVVADPSFLFPEETPGGDW
                     ELFAHSAWGVRCYSSADGLSWRRRRLVVANAMRPFVRAIGGGFLLYVERYRPLALPLT
                     ALPGRRRWSSAIAVSASTDLDSWSTPRTLLEPTLDWMRDERLGESASNPCLTNDGDEW
                     RLYFSASLAWIEDCGFCEPRTIGLAVGPSPTGPFEPTREPVIEPAPGDLAAGSIKAIR
                     VDDGWIGLQNRLHVDAEGRSRSAIFALRSEDGRSWRPAMDEPLLAPKPGWASSHVYAC
                     DCRFREADGLWYLYFNARDG"
     gene            991..1659
                     /locus_tag="PLW80_02505"
     CDS             991..1659
                     /locus_tag="PLW80_02505"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPB65402.1"
                     /translation="MRPSNRSGPGALTDAVYARIRDAIVSAELRPNVRLVESELAVRL
                     DVSRTPVREALLRLEQEGFVRRERGWVVRENEPGEVRSRLECRVAIEGYAARLASQRR
                     DERQLASLRELASAMLEGDREPGRLHALNDAFHGLILEASGNGTLAALHAQTKMNYWD
                     LSVPVVFSAEADRLVDEQHAALLAAIADRDAGEAERVARAHVQGTLDIVLRELTARYP
                     GFSA"
     gene            complement(1669..2097)
                     /locus_tag="PLW80_02510"
     CDS             complement(1669..2097)
                     /locus_tag="PLW80_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HPB65403.1"
                     /translation="MNILIANDHGAVELKTAIVAALAAKGHRVENLGVDTGDSVDYPD
                     MAKLAAERYLAGGFDFGILCCGSGIGISIAANKVDGIRCAQVFDLYTAEMCKRHNDAN
                     FIAFGGRVEYAVPVVDMIERYMAAEFEGGRHRRRVDKLCR"
     gene            2318..>2597
                     /locus_tag="PLW80_02515"
     CDS             2318..>2597
                     /locus_tag="PLW80_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65404.1"
                     /translation="MRNRRSYILALGIAAFGIVALGVVWAQDSGSGGEASVQSAYDIF
                     GLSPDDTGDDAGDEAPTGDEALESLATDAVPEPAAEPVPEAGAEPIAEP"
BASE COUNT          418 a          880 c          915 g          384 t
ORIGIN      
        1 gccgtcgcgc gcgttgaagt acaggtacca cagtccgtcg gcctcgcgga aacggcaatc
       61 gcaggcgtag acgtgggacg aggcccagcc aggcttcggc gccaggagcg gctcgtccat
      121 cgccggccgc caggagcgtc cgtcttcgga gcgcagcgcg aagatggccg agcgcgagcg
      181 accttcggcg tcaacgtgga ggcggttctg aagccctatc cagccgtcgt ccacccgtat
      241 ggccttgatc gagcccgcgg cgaggtcccc gggggccggc tcgatgaccg gctcccgcgt
      301 cggctcgaag ggtccggtcg gcgacggccc gacggccagg cctatggtcc tgggctcgca
      361 gaagccgcag tcctctatcc aggccagcga cgcggagaag tacaggcgcc actcgtctcc
      421 gtcgttcgtc aggcaggggt tcgacgccga ctcgccgagc cgttcgtcgc gcatccagtc
      481 cagggtgggc tcgagcaggg ttctgggcgt cgaccaggag tcgaggtcgg tagaggccga
      541 aaccgctatg gccgaactcc accgccgccg gccgggcaag gcggtcaggg gcagggcgag
      601 cggacggtat cgctcgacgt agaggagaaa cccgccgccg atggcgcgga cgaagggccg
      661 catcgcgtta gccacgacga ggcggcgcct gcgccacgat agcccgtcgg ctgacgagta
      721 gcagcgcacg ccccaggccg agtgggcgaa cagttcccag tcgccgcccg gggtctcctc
      781 ggggaacagg aagctcgggt cggcgacgac cggcgagaag gacggcggtt cgatgagcgg
      841 gccctcggtc tcggcccagt ccaacgctat gaaatcgtta atggtcatcc cgaaagcata
      901 gcgccgccca taggcgagcg caagctccgc ccgacgacgc cgccttgacg ccgtagcgcg
      961 gctgggccgc ggttggtata ctgcgtgacc atgcgaccat cgaaccgatc cggccccggg
     1021 gccttgaccg acgcggtcta cgcccgcatc cgagacgcca tcgtgagcgc cgagctcagg
     1081 ccgaacgtcc gcctcgtcga gagcgagctg gccgtcaggc tcgacgtctc gcggacgccg
     1141 gtgcgcgagg ccctcctgcg gctggagcag gaaggcttcg tgaggcgcga gcggggctgg
     1201 gtggtacgcg agaacgagcc cggggaagtg cgctccaggc tcgagtgccg cgtggccatc
     1261 gagggctacg cggcccggct ggccagccag cggcgtgacg agaggcagct cgcgtcgctg
     1321 cgcgagctgg cctccgcgat gctcgaaggc gaccgcgagc cgggcaggct ccacgcgctg
     1381 aacgacgcct tccatggact gatactcgag gcgtcgggca acgggacgct ggccgcgctg
     1441 cacgcgcaga ccaagatgaa ttactgggac ctcagcgtgc ccgtcgtatt ctccgccgaa
     1501 gccgacaggc tggtcgacga gcagcacgcg gccctgctcg ctgccatagc cgaccgcgac
     1561 gccggcgagg cggagcgcgt ggccagggcc cacgtacagg gaaccctcga catcgtgctc
     1621 cgcgagctca cggcgaggta cccgggcttc tcggcctagc ggctcatcct agcggcacag
     1681 cttgtcgacg cgcctccggt ggcgtccgcc ctcgaactcg gcggccatgt agcgctctat
     1741 catgtcgacg accggcacgg cgtactcgac tcggccgccg aacgcgatga agttggcgtc
     1801 gttgtggcgc ttgcacatct cggcggtgta caggtcgaag acctgggcgc agcgtatgcc
     1861 gtcgaccttg ttcgcggcta tcgagatgcc aatgccggag ccgcagcaca ggatgccgaa
     1921 gtcgaagccg ccggccaggt atcgctcggc cgccagcttc gccatgtccg gataatccac
     1981 cgagtcaccc gtgtcgacgc cgaggttctc gacccggtgc cccttggcgg cgagggctgc
     2041 gacgatggcc gtcttgagct cgaccgcgcc gtggtcgttg gctatcagga tgttcatgtt
     2101 ccgtcctccg cccgccatcc taccatggac gctcgatctg ggcgatgccc ccttcagtcc
     2161 gtatctcgtc gattatccgc aggcggtacc cgatgaagcg gtccccgagg tagcggtccc
     2221 ggaacctagg atagcggcta aacgacgggg cgttccaggc cgatactgaa gcaggggcat
     2281 cccgcgaggg ttcgcccgtc ccgagaggag ccgactcatg cgcaatcgtc gttcgtatat
     2341 cctcgccctg ggcatcgccg ccttcggcat cgtcgccctc ggggtcgtgt gggctcagga
     2401 ctcaggttct ggcggagagg cttccgtcca gagcgcctac gatatcttcg gcctgagccc
     2461 cgacgatacg ggcgatgatg ccggcgacga ggcccctacg ggggacgagg ctctcgagtc
     2521 gctggccacg gacgccgtgc cggagccggc cgcggagcct gtacccgagg cgggggccga
     2581 gccgatagcc gagccga
//
LOCUS       DAOOLN010000138         2595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_48930_length_2595_cov_2.664173, whole genome shotgun sequence.
ACCESSION   DAOOLN010000138 DAOOLN010000000
VERSION     DAOOLN010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48930_length_2595_cov_2.664173"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1392
                     /locus_tag="PLW80_02520"
     CDS             121..1392
                     /locus_tag="PLW80_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65405.1"
                     /translation="MTIAITDNPALRALLAACCIALSSCGLVEPPVAELLTDVPEMAI
                     YAAAFNTQQERFRVNVSYDDQVAETLEKRADKPSLVIGRYLKSSRSRSSFQALDHLFS
                     DLIVNPSSFYDGLLELGNYEGRQILLPVSFNLPLIVFDRALEPTMPDGFVIDLADLET
                     FGAGLNKSSKASVTNMGFGPRWSPDFLFSAISLLGAGFKEGAPLKWDHAALDSSIAYI
                     RAWSERANESAAKEDEFQFKYLYLPAYRSVEEGRIGFAAMSSSEFFVISEERRASLAI
                     RWLSRSSSIPVEEDIVYAGICRKGEGRQAAEAFIKWFYSEASQRTILEDAKRYRSIES
                     SFGIAGGFSAIRSVNERLFPLYYPSLLGKLPPAGYLSPPNVLPAQWPAMKEAIVLPFL
                     LEQTGPTPPQGDRNQALEAKLQAWLKRRSSD"
     gene            complement(1389..2384)
                     /gene="dusB"
                     /locus_tag="PLW80_02525"
     CDS             complement(1389..2384)
                     /gene="dusB"
                     /locus_tag="PLW80_02525"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPB65406.1"
                     /translation="MNLHVPVRVGSYTTRGNLFLAPVAGYSDAAFRSICADFGCDLAF
                     TEMVSSEALTRGHLKTEVLLAREDNEPDYAVQLFGSGPETMAAAALIVAERWRPAVID
                     VNCGCPVPKIVKTGAGSALMRDPSRIRAIVAAMTGAVKTPVTVKIRLGWDDASVNYLE
                     AATAAVEGGAAAITLHARTRERGYAGTADRQAFFALAEALPVPVFASGDLFSAKAAVD
                     ILSGGSVAGVMFARGAMGDPFIFSRARAALEGLPEPIETAAGRMSVARRHLELSLRHY
                     GERTACVEFRKQACAYLKGVPGGAELRRLAVGCSTLAEYDGFFAAWAATAARGDA"
BASE COUNT          465 a          901 c          782 g          447 t
ORIGIN      
        1 cgtcttgatc tgcatgcgct tagtataccg tttatctccc ggaggcgcta tatgggttgc
       61 ttcgattccg tcaagatagt ccgcttgagc aaggctcgta gtccaggtta ctctcgactc
      121 atgacgatag cgatcaccga taacccggcc ctccgcgccc tgttggccgc ctgctgcatc
      181 gccctgtcat cctgcgggct cgtggagccc ccggtcgcgg agctgctaac ggacgtcccg
      241 gagatggcca tctacgccgc ggccttcaat acgcaacaag agcgcttccg cgtcaacgtc
      301 tcctacgacg atcaagtcgc cgagaccctc gagaaacgag ccgacaagcc gtccctggtg
      361 ataggccgct acctgaagag ctcacgttcc cgctccagct tccaggcgct cgaccacctc
      421 ttctccgacc tcatagtcaa ccccagctcc ttctacgacg gcctgctcga gcttggcaac
      481 tacgaggggc ggcagatcct gctaccggtg tcgttcaacc tgccgctgat cgtattcgac
      541 cgcgcgctcg agccgacgat gcccgacggc ttcgtcatcg acctcgccga cctggagacg
      601 ttcggcgcgg ggttgaacaa atcgtccaag gcttccgtca cgaacatggg cttcggaccg
      661 cgttggtcac cggactttct tttctcggcc atcagcctgc tcggcgcagg cttcaaggaa
      721 ggcgctccac tcaaatggga ccacgcggcc ctggactcga gcatcgccta tatacgcgcc
      781 tggtcggagc gggctaatga atccgccgcc aaggaagacg aattccagtt caagtacctc
      841 tacctgccgg cctaccgatc ggtagaggaa ggccgcattg gcttcgccgc gatgagttcc
      901 tcggaattct tcgtgatctc cgaggaacga agggcctcgc tggccatccg ctggctatcg
      961 cgttccagct cgattcccgt cgaggaagac atcgtctacg ccgggatctg ccgcaaggga
     1021 gagggcaggc aggccgccga ggcgttcatc aagtggttct attccgaggc gagccaacgg
     1081 accatcctcg aggacgccaa gcgctatcgt tcgatcgaat cgtccttcgg catcgccggc
     1141 ggcttttcgg ccatacgatc ggtcaacgaa aggctgttcc cgctctacta cccgtcgcta
     1201 ctcggcaagt tgccccccgc cggctacctt tcgccgccga acgtactccc ggcccaatgg
     1261 cccgcgatga aagaggctat cgtactaccc ttcttgctcg agcagaccgg ccccaccccc
     1321 cctcaaggtg acaggaacca ggccctcgaa gccaagctac aggcctggct taagcgacga
     1381 tcgagcgatt aggcatcgcc acgcgcggcc gtagcggccc aggcggcgaa gaagccgtcg
     1441 tactccgcca gggtcgagca gccgacggcc agccgacgca actccgcgcc gcctggcact
     1501 cccttgagat aggcgcaggc ctgtttcctg aactcgacac aggccgtccg ctccccataa
     1561 tggcggagag acagctccag gtggcgccgc gcgaccgaca tgcgcccggc ggccgtctcg
     1621 atcggctccg gtaggccctc caaggctgcc cgggccctcg agaaaatgaa cgggtcaccc
     1681 atcgctcccc tggcgaacat gacgccggcc acgctaccgc ccgacaggat gtccacggcc
     1741 gccttggcag agaacaggtc ccccgaggcg aacaccggca cgggcagagc ctccgccagg
     1801 gcgaagaacg cctgtcggtc ggcggttccg gcatagccgc gctccctcgt gcgcgcgtgc
     1861 agggtgatag cggccgcgcc gccctcgacc gccgcggtcg ccgcctcgag gtaattcaca
     1921 ctggcgtcgt cccagcccag gcgaatcttc acggtcacgg gcgtcttgac cgctccggtc
     1981 atcgcggcga cgatggcccg tatcctcgac gggtcgcgca tcagggccga tccggccccc
     2041 gtcttgacta tcttaggcac cgggcagccg cagttaacgt cgatcacggc cgggcgccag
     2101 cgttcggcga cgatgagggc ggccgccgcc atcgtctcag gccccgagcc gaacagctgg
     2161 acggcgtagt ccggttcgtt gtcctccctc gccagcagga cctcggtctt gagatggccg
     2221 cgggtcaggg cctcggaact gaccatctcg gtaaaggcca ggtcgcaacc gaagtcagcg
     2281 cagatcgaac ggaacgccgc gtcggagtat ccggccaccg gggccaggaa caggttgcct
     2341 ctcgtcgtat acgacccgac gcggacaggt acgtgcaggt tcatggtgtc tccgggaaga
     2401 atgtagctaa aaaaggcgcc cgtaccacgg ggcgcctcct atcgtcgcgg cgcggcgcgt
     2461 acggccggcc cacggtatca ttcgggcacg cggaacagcc tgcaggcgtt ctggtaggtc
     2521 gcctcggcca catcctcgac cggcaagtcg aggagcatgg ccaggtcgtc gaccgtggcg
     2581 ggcaggtact cgggt
//
LOCUS       DAOOLN010000139         2586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_49178_length_2586_cov_2.751087, whole genome shotgun sequence.
ACCESSION   DAOOLN010000139 DAOOLN010000000
VERSION     DAOOLN010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49178_length_2586_cov_2.751087"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="PLW80_02530"
     CDS             <1..1065
                     /locus_tag="PLW80_02530"
                     /inference="COORDINATES: protein motif:HMM:NF033751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pallilysin-related adhesin"
                     /protein_id="HPB65407.1"
                     /translation="IVCTGMDDANNQTMTVYWKSQDAYRDQQAAYIKILEQAGSAVLV
                     ESRDRPDSYKLGQSNAESWPVSVWKEDAGSGNYLDQIKETWQWSFADEAYVLAGTVSI
                     PGASIARQKAESILDGTAETFKAFLDGTWYKESSDPLSSGALFITFQSREEAVLFSGE
                     GLTEVYTWENSNPTRYGLYIASRNQSVRNLRRLMDIELASADSVNVRVFQDYRIKADI
                     SGRWDGRYRRLTPNLAAAFRRSPAMARSAQSDIGGLYESDDGSSLSLVGSEYVLKSPD
                     GEQRGAFSIYELQGRVVLDTRRIADGDGESVRRSWVATVAESRGPDGQSVQTLTLEEA
                     TIGILGIEPIQSPTLSFTRR"
     gene            complement(1077..2072)
                     /locus_tag="PLW80_02535"
     CDS             complement(1077..2072)
                     /locus_tag="PLW80_02535"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPB65408.1"
                     /translation="MRSSVPAIALLTIVVLTMVPLRAKCQGAEAVAALERLTASASAP
                     DLAARARTEFDTLSRIDEVAWLAGALAAKAVVPSQRGNLLVEQASALELLGRYGEASV
                     VWEVAATAVPGPADAKRLLAAAACALAAGDVDSAGALAKAVGFASPEPRTAELARLVS
                     GWAALSRGERAVAAGIAESILGRPSSLEAAALMLARAAAEGQAALAYETRLAALGSRP
                     EADSSAIAALSLASRSVIKEPAELSEPAVAADPVQPVAADGARKASYQVGAFRERANA
                     DALVSRLGGLGVTAKVARKADSGLFVVVAEGGVDPARTVLILKDAGFEAWAVEGP"
     gene            complement(2080..2445)
                     /locus_tag="PLW80_02540"
     CDS             complement(2080..2445)
                     /locus_tag="PLW80_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65409.1"
                     /translation="MMPWKLLAFIAVMAIVLVFIGFNLDNRCDISLVVVTFTSVPVVI
                     TILVTYILGLLSALVLSIGRRPSSSRRGIPLRQVAPSVSEQPGADGEAPFKPSASSSS
                     DTQPAGSGSKRPSKGKKRG"
     gene            complement(2442..2570)
                     /locus_tag="PLW80_02545"
     CDS             complement(2442..2570)
                     /locus_tag="PLW80_02545"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HPB65410.1"
                     /translation="MLGRTDQDMMVVFPASAERIGSFARVRLESIRGNTFKAVEVR"
BASE COUNT          493 a          850 c          835 g          408 t
ORIGIN      
        1 atcgtatgca cgggcatgga tgacgccaat aaccagacca tgacggtgta ttggaagtcg
       61 caggatgcgt atcgagacca gcaagccgcc tacatcaaga tcctcgagca ggcaggatcg
      121 gccgtactcg tcgagagccg cgataggccg gacagctata agctcggcca gtcgaacgcc
      181 gagagctggc ccgtctccgt ctggaaggag gatgcgggct cgggtaatta cctcgatcag
      241 atcaaggaaa cctggcaatg gagcttcgcc gacgaagcct acgtgctggc cggtacggtg
      301 agcatccccg gcgccagcat cgctcggcag aaggctgaat cgatcctcga cggcacggcc
      361 gagaccttca aggccttcct cgacggcacc tggtacaagg aatcgagcga cccgttgtcc
      421 tcgggggcct tgttcatcac cttccagtca agggaagagg cggtgctttt ctccggcgaa
      481 gggctgaccg aagtatacac ctgggagaac tccaacccaa cgcgctatgg cctttacata
      541 gcctcgcgga accagtcggt caggaatctg cgccggctca tggacatcga gctcgcctct
      601 gcagactcgg tcaacgtcag ggtcttccag gattaccgca tcaaggccga catatccgga
      661 agatgggacg gcaggtaccg caggttgacg cccaacctcg ccgcggcctt caggcgctcg
      721 ccggccatgg ccaggagcgc ccaatccgat ataggcgggc tctacgagtc ggacgacggc
      781 tcgtcgctgt cgctggtcgg ctcggaatac gtgctgaaaa gccctgacgg agagcagcgc
      841 ggcgcgttct caatctatga gctacagggc cgagtcgtcc tcgacacgag gcgtatcgcc
      901 gacggggacg gggagagtgt caggcgctcg tgggtcgcga ccgtggcgga gtcgcgcggc
      961 cccgacggcc aatcggtaca gacactgacg ctggaggaag cgacaatagg catcctcggg
     1021 atcgagccga tccagtcgcc gacgctgtcg ttcacgcggc gctagtggcc ttcgggctag
     1081 gggccttcta cagcccaggc ctcgaagccg gcatccttga ggataagcac cgtcctcgcc
     1141 ggatcgaccc cgccctcggc tacgactacg aacagccctg aatcggcctt acgcgccacc
     1201 ttcgccgtta cgccgagccc cccgagcctc gaaacgagag cgtcggcgtt ggcccgttcc
     1261 ctgaacgctc ctacctggta ggaggccttc cgagcgccgt ctgccgcgac cggctgaacc
     1321 ggatctgccg ctaccgccgg ctcagagagt tcggcaggct ccttgatgac cgaccgcgag
     1381 gcgagggaca gcgctgcgat agccgaggag tcggcctcgg gacgcgaacc caaggccgcg
     1441 agccttgtct cgtaggccag cgctgcctgg ccctcggcgg ccgctcgagc cagcatcagc
     1501 gccgcggcct ccagcgacga aggacgcccc aggatcgact cggcgattcc ggcggccacg
     1561 gctcgctcgc ccctcgacag cgctgcccaa cccgatacga ggcgggctag ctcggcggta
     1621 cgcggctcgg gcgaggcgaa tcctaccgcc ttggcgaggg cgccggcgga gtcgacgtcc
     1681 cccgcggcta gcgcgcaggc cgcggcggct aggaggcgct tcgcgtcggc tggaccgggt
     1741 acggcggtgg ccgcaacctc ccatacgacc gaggcctcgc cgtagcgccc cagaagctcg
     1801 agcgcggagg cttgctcgac gagaaggttc ccacgttggg acggaaccac ggccttggcg
     1861 gccaaggcac cggccaacca ggctacttcg tcgattcggc tcagcgtatc gaactcggtt
     1921 cgagcccgcg ccgccaagtc gggggctgag gccgacgcgg tcaagcgctc gagcgctgcg
     1981 acggcttcgg cgccttggca cttggcgcgg agcgggacca tcgtcaagac gacgatggtc
     2041 agaagtgcga tcgccggcac ggaggagcgc atcacgagct cagcctctct tctttccctt
     2101 cgaggggcgt ttcgaaccgg atccggcagg ttgcgtatcg gaggaggatg aggctgacgg
     2161 cttgaacggc gcctcgccat ccgcgcctgg ctgctcggaa acggacggcg cgacttgcct
     2221 gagcgggatg ccgcgcctcg acgacgaggg gcgcctgcct atcgagagga ccagggccga
     2281 tagcagcccc agtatatagg tgaccaggat ggttattacc accggtacag acgtgaaggt
     2341 gacgactacc agcgatatat cgcagcgatt atccaggtta aacccgatga acaccagaac
     2401 gatcgccatc accgctatga aagccagtaa cttccagggc atcatcgcac ctccacggcc
     2461 ttgaatgtat tccctcgtat cgactcgagc cgtaccctgg cgaacgaacc gatacgctcg
     2521 gccgacgccg ggaagacgac catcatgtcc tggtcggtac gcccgagcac ctcgtcgcct
     2581 cttcgt
//
LOCUS       DAOOLN010000140         2577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_49429_length_2577_cov_2.643537, whole genome shotgun sequence.
ACCESSION   DAOOLN010000140 DAOOLN010000000
VERSION     DAOOLN010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49429_length_2577_cov_2.643537"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PLW80_02550"
     CDS             <1..319
                     /locus_tag="PLW80_02550"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HPB65411.1"
                     /translation="REDGRDAFVEAYCPELSPASALLDPEREGAVLAALAKAPSKLFV
                     VQLQDLLDLSDRYRSDDPSDDRVNVPGSVDEFNWTWRMGPGVEELERDRAWTALVRAG
                     CGR"
     gene            350..>2577
                     /locus_tag="PLW80_02555"
     CDS             350..>2577
                     /locus_tag="PLW80_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB65412.1"
                     /translation="MRPIVAIAVRNLGVLEHVSRELRSRVTGAEITTIVGDRAFRETI
                     ARGAAASIDALVADAECAELIAALSKPEDLPLVGVIAPASSGAGLPRAIADAGFPMLR
                     LCAEPRPYAEVAAWVNGAVASIARAGGRAHDSVDYGQRYEDLVQALPDIVYELDVDGV
                     ITFINDSVSMLGYAPGDLIGKHYSVLLHDEDAAAVDRDRVLVDYVGYRTGVALSPKLF
                     NERRSIERRTTDLEVRLKRKRGSKNGLPELIGSVISYGEISSAGEYGRDGAREFKGSV
                     GIIRDVTLRRKSEETLRKLYQAIDQLGSCVFVLNHAFEVEYVNPAFFMLTGFAPPDVI
                     GGNVFRFLAFLPDTVDAVRKRVQDGFEAREEALVPRMGGGQFWASLSIAPVRTPSGAI
                     THAIAVMDDISARRSMEELLRGARLEAEEASRSKSRFISSMTHELKTPIASIIAAARS
                     LQGGEGGPVALILDNALRLLDILNGILDYVRSESDVGAVRRVSFRLGSFMERACGPHR
                     ATAVGKGLSFSLEADSDESVESDPDRLGRAIEILLDNAVRYTDSGSIRVTARIERLEG
                     NVPHLIVHVADTGPGIAAQDRERIFKPFSRAEHAARGGPGGSGVGLALARNIMQILGG
                     EVRLDTGFGIGSDFSLILPTASPVRTEAAEAAGYTLLLVDDNEVNLEYMRTLVENRGF
                     RVLCAESAAEAFRVLEAHYVDACILDIQMPGYSGIELAKGIRAYAGPRYSSDLPLFAM
                     TA"
BASE COUNT          444 a          828 c          865 g          440 t
ORIGIN      
        1 acgggaggac ggcagggacg ccttcgtcga ggcctactgt cccgagctga gtcccgcttc
       61 ggcgctactc gacccggagc gtgaaggtgc cgtgctcgcg gcgctggcga aggccccgtc
      121 caagttgttc gtcgtccagc tccaggacct gctcgaccta tccgatcgct ataggtcgga
      181 cgatccgagc gacgataggg tcaacgtgcc gggcagcgtg gacgagttca actggacctg
      241 gcgcatgggg cccggcgtgg aggaactcga gcgcgaccgt gcgtggaccg ctctcgtacg
      301 ggccggctgc ggccgataag gtagtatggc tcagagtaca aaagatgcga tgcgtccgat
      361 cgtcgccata gcggtcagaa atctgggagt cctcgagcac gtctcgcgcg agctccgctc
      421 aagggtgacg ggagccgaga taacgacgat agtcggcgac cgggctttcc gcgagacgat
      481 agcgcgcggc gccgccgcgt ccatagacgc gctcgtggcg gacgcggagt gcgccgagct
      541 gatagcggcg ctatcgaagc ccgaggatct gccgctggtc ggagtcatcg cgcccgcttc
      601 gagcggtgcc ggcctgcctc gggctatcgc ggacgccggt ttccccatgc tacggctatg
      661 cgccgagccg aggccctacg ccgaggtcgc ggcctgggtg aacggcgccg tcgcgtcgat
      721 agccagggcc ggggggcgcg cccacgattc ggtcgactac gggcaacgat acgaggacct
      781 ggtccaggcc ttgcccgaca tcgtctacga gctcgacgtc gatggcgtca tcaccttcat
      841 taacgactcg gtctccatgc tcggctacgc cccgggcgat ctcataggca agcactactc
      901 ggtcctgctc catgacgagg acgcagcggc cgtggaccgg gatagggtcc tggtcgacta
      961 cgtcggttac cggaccggcg tcgccctcag cccgaagctg ttcaacgagc gccgtagcat
     1021 cgagcggcgt acgaccgatc tcgaggtcag gctgaagcgc aaacgcggct ccaagaacgg
     1081 gctccccgag ctgataggca gcgtcatctc gtacggcgag atctcgtcgg ccggcgaata
     1141 cggtcgcgac ggagccaggg aattcaaggg ctcggtcggc ataatccgcg acgtcacgct
     1201 acggcgcaag tcggaggaga cgctccgcaa gctgtaccag gcgatcgacc agctcggctc
     1261 gtgcgtcttc gtgctcaacc atgcgttcga ggtcgagtac gtcaaccctg ccttcttcat
     1321 gctgacgggc ttcgcgccgc cggacgtgat aggcggcaat gtcttcaggt tcctggcctt
     1381 cctgccggat acggtcgacg ccgtccgcaa gcgggtccag gacggcttcg aggcgcggga
     1441 agaggctctc gtcccgcgta tgggcggcgg acagttctgg gcgtccctgt cgattgcgcc
     1501 ggtcaggacg ccgtccgggg cgatcaccca cgccatcgcc gtcatggacg acatcagcgc
     1561 taggcggtcc atggaggagc tactgagggg cgcgcgtctc gaagcggagg aggcgagccg
     1621 atcgaagtcg cgcttcatct cgagcatgac ccacgagctt aagacgccga tcgcgagcat
     1681 catcgccgcc gctcgttcgc tccagggcgg cgagggcggt cccgtagcgc tcatcctcga
     1741 caatgcccta cgtctcctcg atatactgaa cggcatactc gattacgtcc gttcggagag
     1801 cgacgtcggg gccgtacgac gtgtctcctt ccggctcggc tcgttcatgg agcgggcctg
     1861 cggcccacat cgcgcgacgg ccgtggggaa agggctatct ttctcgctcg aggcggactc
     1921 ggacgagagc gtcgagagcg acccggacag actcggccgc gccatcgaga tactgctcga
     1981 taacgcggtg cgctacaccg actcgggctc gatacgggta acggccagaa tcgagcgact
     2041 cgaaggtaac gtgccgcacc tgatcgtcca cgtggccgat acgggaccgg gcatagcggc
     2101 tcaggatagg gagcgcatat tcaaaccctt ctccagggcg gagcacgcgg cccgaggcgg
     2161 ccccggaggt tctggcgtcg gtctcgcgct cgccaggaac atcatgcaga tcctcggtgg
     2221 tgaggtacgg ctcgataccg gttttggcat aggcagcgat ttctcgctga tcctgccgac
     2281 cgcttcgccg gtcaggacgg aagcggcgga ggcggccggc tatacgctac tcctcgtcga
     2341 tgataacgag gtgaacctcg agtacatgag gaccctggtg gagaaccgcg gcttccgcgt
     2401 gctttgcgcc gagagcgcgg cggaggcgtt ccgtgtgttg gaagcccatt acgtcgacgc
     2461 ctgcatcctc gatatccaga tgcccggcta ttccggcatc gagctggcca agggcatccg
     2521 cgcttacgcc ggtccacggt actcctccga cctgcccctg ttcgccatga ccgccca
//
LOCUS       DAOOLN010000141         2576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_49457_length_2576_cov_2.566442, whole genome shotgun sequence.
ACCESSION   DAOOLN010000141 DAOOLN010000000
VERSION     DAOOLN010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49457_length_2576_cov_2.566442"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PLW80_02560"
     CDS             complement(<1..662)
                     /locus_tag="PLW80_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB65413.1"
                     /translation="MPEVRPDAAPPNDAPRSPADAAPPAAGTPAAGTPAIIELRDVRA
                     SSGGFEILDGACVAFPEGLCSVVMGPAGSGKSTLLKVAAGLVVPDSGSVLYRGADLAR
                     FGRKDEAAFRAATGFVFQDAALWADQNILMNVTMPLRVHKAWLGPSAMAELARETLGR
                     LGYDEGMAYRPAELSAGEQKLVSIARAVIHEPQIVLMDDPTASLDEDAAERVYALIAK
                     LR"
     gene            complement(655..1437)
                     /locus_tag="PLW80_02565"
     CDS             complement(655..1437)
                     /locus_tag="PLW80_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB65414.1"
                     /translation="MAIISALGRAARTKASGLLYGLGFFYNVLKETGRFFRRAQVGYK
                     VLIMQILFTGYEALAINAIMAIAIGAAINVIGSSLLPQFGQSQLMYSILIIVITRELG
                     PLLTAFVITARSGTAIATQLGTMVVNHEIEAYMAVGLNPISYLVVPRFIGVVVSMLVL
                     TVYFNIFGLLGSFIVVQVVNPTPIGEYLGALMSTLTVADVLSGLTKALVFGVIVSVVS
                     TYQGFAVNRASTEIPVAGIKAVGSSFILIILADVIVTLVQYA"
     gene            complement(1477..2259)
                     /locus_tag="PLW80_02570"
     CDS             complement(1477..2259)
                     /locus_tag="PLW80_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPB65415.1"
                     /translation="MKPECREHREKIAQHLLGDLTAEESRSLETHLAACPGCRSELDR
                     YAEVLHRLPAAGDEPDGLFELTAWRAAPDGRTRAVAKRALQPAEIVPLGDVPDAALAA
                     FIPALGPFSFADVDYDPFRSIASSLDRALIRPAVPQLALWAAIEGDLFRFAYDAGFTA
                     ALETKRGTAEWARFAALNELYRDLVRAILPFGAAPPGWRPWLTGDGRNGAAARASPAG
                     TASGAAARALARALGRGLSSIRPALMKRAGTANCFTLATIGG"
     gene            complement(2280..>2576)
                     /locus_tag="PLW80_02575"
     CDS             complement(2280..>2576)
                     /locus_tag="PLW80_02575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPB65416.1"
                     /translation="PPGIHISLEDERAAALPVAQLAVPEPQDAELLRFEERQRVRRAL
                     SALPADQRAVVELKFFQDRTFEEIAAILETPLSTIKSRLYTGLELLKDRLGARD"
BASE COUNT          414 a          842 c          923 g          397 t
ORIGIN      
        1 tcgcgcagtt tggctatcag ggcgtagacc cgctcggccg cgtcctcgtc caggctggcc
       61 gtcgggtcgt ccatgagcac gatctgcggc tcgtggatca cggcgcgcgc gatggagacg
      121 agcttctgct cgccggccga cagctccgcc ggccggtagg ccatgccctc gtcgtagccg
      181 agcctgccga gcgtctcgcg cgccagctcg gccatcgcgc tcgggccgag ccaggccttg
      241 tgcacgcgca gcggcatcgt cacgttcatc aggatgttct ggtcggccca gagcgcggcg
      301 tcctggaaca cgaagccagt ggcggcgcgg aacgcggcct cgtccttgcg gccgaagcgc
      361 gccaggtcgg cgccgcggta gagcaccgaa cccgagtcgg gcacgacgag gccggcggcc
      421 accttgagca gggtgctctt gcccgagccg gccggcccca tcaccacgga gcataggccc
      481 tcggggaagg cgacgcaggc gccgtcgagt atctcgaagc cgccggacga tgcgcgcacg
      541 tcgcgtagct cgatgatcgc cggagtaccg gcggccggag taccggcggc cgggggcgcg
      601 gcgtcggccg gtgaccgggg ggcgtcgttc gggggcgcgg cgtcgggtcg gacctcaggc
      661 atactgcacc agggtgacga tcacgtcggc caggatgatc aggatgaagc tcgagccgac
      721 cgccttgatg ccggcgaccg gaatttcggt gctggcccgg ttgaccgcga agccctggta
      781 ggttgacacg accgagacga tcacgccgaa gaccagggcc ttggtcaggc cggacaggac
      841 gtcggccacc gtcagcgtcg acatgagggc gccgaggtac tcgcctatcg gcgtcgggtt
      901 gaccacctgc accacgatga acgagccgag gagcccgaag atgttgaagt agacggtcag
      961 cacgagcatc gagacgacca cgccgatgaa gcgcggcacc acgaggtagg atatcgggtt
     1021 gagcccgacg gccatgtagg cctcgatctc gtggttgacg accatcgtgc cgagctgggt
     1081 ggcaatggcg gtgcccgagc gcgcagtgat cacgaaggcg gtcaggagcg gcccgagctc
     1141 gcgggtgatc acgatgatca ggatcgagta catgagctgg ctctggccga actgcggcag
     1201 gaggctcgag cctatcacgt tgatcgcggc gcctatcgct atggccatga tcgcgttgat
     1261 cgcgagggcc tcgtagccgg tgaacaggat ctgcatgatc aggaccttat agcccacctg
     1321 cgcgcgccgg aagaagcggc cggtctcctt gaggacgtta tagaagaagc cgagcccgta
     1381 caggaggccc gaggccttgg tacgggccgc gcgcccgagc gccgatatga tcgccatgct
     1441 acctcgcttc gagtatcggc ccgccgggcc ttccgttcaa ccgcctatcg tggcgagcgt
     1501 gaagcagttc gcggttcctg cccgtttcat cagggcggga cggatcgacg ataggcccct
     1561 accgagggcg cgggccaggg cccgcgccgc ggcgccgctt gcggttccgg ccggcgatgc
     1621 gcgggcggcc gctccgttac gtccgtcgcc ggtcagccag ggccgccaac cggggggagc
     1681 cgcgccgaac ggcaggatgg ccctcaccag gtcgcggtac agctcgttga gagcggcgaa
     1741 gcgggcccat tcggccgtgc cgcgtttcgt ctcgagagcc gcggtaaagc cggcgtcgta
     1801 cgcgaaccgg aacaggtctc cctcgatcgc ggcccagagc gccagctgcg ggacggcggg
     1861 acggatcagg gcgcggtcga gcgacgaggc tatcgagcgg aaagggtcgt aatcgacgtc
     1921 ggcgaacgag aaaggcccaa gggcggggat gaaggccgcg agcgcggcgt ccgggacgtc
     1981 gcccaacggg acgatctcgg cgggctggag cgcccgcttg gcgacggcgc gggtccggcc
     2041 gtcgggcgcg gcgcgccagg cggtcagttc gaagaggccg tcgggctcgt cgcccgccgc
     2101 cggcaaccga tgcaggacct ctgcatatcg gtccagttcc gacctgcagc cggggcaagc
     2161 ggcaaggtgc gtctcgagcg agcgcgattc ttccgccgtc aggtctccca gaaggtgctg
     2221 cgcgatcttt tcccgatgtt ccctgcattc cggtttcatg ttcccccccc ccttgcgcat
     2281 caatccctgg cgcccagccg gtccttgagc agttccagcc ccgtatagag ccgggacttg
     2341 atcgtgctca gcggcgtttc aaggattgcc gcgatttcct cgaatgtcct gtcctgaaaa
     2401 aatttcaatt cgacgacggc ccgctggtcc gccggcaggg cggaaagggc gcgccgcacc
     2461 ctctgccgct cttcgaaccg gagcagttcg gcgtcctggg gttccggcac cgccagttgc
     2521 gcgacgggca atgccgcggc tctttcgtct tcgagcgata tatggatgcc cggggg
//
LOCUS       DAOOLN010000142         2574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_49509_length_2574_cov_2.657404, whole genome shotgun sequence.
ACCESSION   DAOOLN010000142 DAOOLN010000000
VERSION     DAOOLN010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49509_length_2574_cov_2.657404"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PLW80_02580"
     CDS             complement(<1..724)
                     /locus_tag="PLW80_02580"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65417.1"
                     /translation="MGIARRLFLGLISSLLLALSLPNELFSWGAPALGLVALAPLFVA
                     LAESGSYRESFAIGSTFGGLAHGLSSYWLWFFKDFRFWTLGTTVIAYMIVYGTLGLYL
                     RGTLKRGGLARPLIFAMAWAVFEWGKSSGFLGYPWGLVAYSWNTVLPAVQIAESTGVY
                     GLSFALAFVSASIGEVLCGPPARLPGPADGVAERTPAGRLSAVVPPRPAWPDGRYASL
                     VNLVVAVLCLVAIYAYGLVALER"
     gene            834..1328
                     /gene="coaD"
                     /locus_tag="PLW80_02585"
     CDS             834..1328
                     /gene="coaD"
                     /locus_tag="PLW80_02585"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPB65418.1"
                     /translation="MVKAVFPGSFDPPTFGHLNIIERARTIFERVLVVVAVNRDKRYL
                     FDADERMAFMKELVAPWPNVEVHRCDTLIVDFAREHACRVLLRGVRSVQDFSYEFELS
                     ILNKGVGPEIETVFMPTDQKFFVLRSSAIKELASFGGDVSAMVPPVVAKALERFRVGA
                     HSST"
     gene            1401..1589
                     /gene="rpmF"
                     /locus_tag="PLW80_02590"
     CDS             1401..1589
                     /gene="rpmF"
                     /locus_tag="PLW80_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPB65419.1"
                     /translation="MAVPRCNTSKARSRRRRSINMKLVAPTIVSCGNCGNAVLFHRVC
                     PKCGFYRGRQVLTPETNA"
     gene            1602..1835
                     /gene="acpP"
                     /locus_tag="PLW80_02595"
     CDS             1602..1835
                     /gene="acpP"
                     /locus_tag="PLW80_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPB65420.1"
                     /translation="MDELYEKIKKIIADKLEVEEAKINLDASFRQDLGADSLDTYELV
                     YGIEEELKIRIPDEKANEFETVRDAYEFIKSQV"
     gene            1893..>2574
                     /gene="rnc"
                     /locus_tag="PLW80_02600"
     CDS             1893..>2574
                     /gene="rnc"
                     /locus_tag="PLW80_02600"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPB65421.1"
                     /translation="MSFRYDDIGRDRLEALAAFQRSLRFRFKDLSLLNRALTHRSHVN
                     EDPASRGNNERLEFLGDAVLGMATASRLFIALGDKSEGDLARIKSFVVSEDTLSDIAS
                     RLGVDGVLLVGKGEERSGGRNKKAILADAMEAVFGAAYLDAGFDKAKDLVLSLIDPEI
                     TKVLTDRHRKDYKTLIQEYAQKWHRCYPVYTLERKAGPDHDRTYWIRCELLGASYGPA
                     AGKTKKDAE"
BASE COUNT          524 a          793 c          823 g          434 t
ORIGIN      
        1 gccgctcgag cgctaccagg ccgtaggcgt agatagccac gaggcatagg actgcgacga
       61 cgaggttgac taggctagcg tagcgaccgt ccggccaggc cggccgaggc ggaacgacgg
      121 cggagaggcg acccgccggc gtccgctcgg ctacgccatc agccgggcca ggcaagcgcg
      181 cgggggggcc gcagagaacc tcgccgatcg aggccgagac gaaggccaac gcgaacgaca
      241 gtccgtacac gccggttgac tcggcaatct gcacggcggg aagcaccgtg ttccagctat
      301 aggcgacgag cccccatggg tagccgagaa agccggacga cttgccccac tcgaagacag
      361 cccaggccat cgcgaagatg agcggccgcg ccagcccacc tcgcttgagt gtcccgcgca
      421 ggtatagccc cagggtcccg tagactatca tgtaggcgat caccgtcgtg ccgagcgtcc
      481 agaacctgaa gtccttgaag aaccatagcc agtagctgga caggccgtgg gccaggccgc
      541 cgaacgtaga tcctatggcg aacgactcgc ggtacgaccc ggattccgcc agggcgacga
      601 acagcggcgc gagcgccacg aggccaaggg ccggggcgcc ccatgagaac agctcgttgg
      661 ggagcgatag tgccaggagg aggctcgaga tcaagccgag gaagagtcgc cgcgcgatgc
      721 ccatgggcac atgatacacg ctgatcgagc ccagcgccag cgatggccgt cggcgggcgc
      781 gtatacgtgg aaaacacctt gacggcgctc gcctacccgg atatggttcg tccatggtca
      841 aggccgtctt tcccggttcg ttcgatcccc cgaccttcgg gcacctgaac atcatcgagc
      901 gcgcccgtac gatattcgag cgggtcctgg tggtcgtcgc cgtcaaccgc gacaagcgct
      961 atctgttcga cgccgacgag cgcatggcgt tcatgaaaga gctggtcgcg ccctggccga
     1021 acgtcgaggt gcaccgctgc gacaccctga tagtcgattt cgcgcgggag cacgcctgca
     1081 gggtgctatt gcgcggcgta cggagcgttc aggacttctc gtacgagttc gagttgtcta
     1141 tcctgaataa gggagtcggc ccggagatcg agacggtctt catgcctacg gatcagaaat
     1201 tcttcgtact gcgctcgagc gccatcaagg agctcgcctc cttcggcggc gacgtcagcg
     1261 ccatggtgcc gccggtcgtg gccaaggcgc tcgagcgttt cagggtcggt gcccactcct
     1321 cgacttgaca aatatccggt atttcgttat tgtgcgccgg tatccgttac aggcacattt
     1381 tgtgggaatg aggttatagt atggctgtac ccagatgtaa tacttcgaag gcacgttcgc
     1441 gcaggcgccg ctctatcaat atgaagctcg tcgcgcccac gatcgtttcc tgcgggaact
     1501 gcggcaacgc cgtactgttc cacagggttt gcccgaagtg cggcttctat cgcggcaggc
     1561 aggttttgac ccccgaaacc aacgcgtaaa ggagactcgc catggacgag ttgtatgaga
     1621 agatcaagaa gataatcgcc gacaagctcg aggtcgaaga ggcaaagatc aacctcgacg
     1681 ccagctttag gcaggatctt ggcgccgaca gcctcgatac ctacgagctg gtctacggca
     1741 ttgaggaaga gctgaagatc cgcatccccg acgagaaggc caacgagttc gagaccgtcc
     1801 gggacgcgta cgaattcatc aagtcccagg tgtgacgtag ccgggcgggc ttgctggccc
     1861 gtccgcaatc gtcggtcaag gaggccgcga tcgtgtcctt ccgttacgac gacattggtc
     1921 gtgatcggct ggaggcgctc gcggcctttc agcgttcttt gaggttccgc ttcaaggatc
     1981 tatcgctcct caaccgggcg ctgacccacc gctcccacgt caacgaggac cccgcgtcgc
     2041 gaggtaacaa cgagcggctc gaattcctcg gcgatgccgt gctgggcatg gctaccgcct
     2101 cgcgcctttt catcgccctc ggcgacaagt cggagggcga cctcgccagg atcaagagct
     2161 tcgtcgtctc agaggatacc ctatcggaca tagcctcgag gctaggggtc gatggggtgt
     2221 tgctcgtagg caagggcgag gaacgctcgg gcgggcgcaa caagaaggcc atcctggccg
     2281 acgcgatgga ggccgtcttc ggcgcggcct acctcgacgc cggcttcgat aaggccaagg
     2341 acctcgtact gtcgctcatc gatcccgaga taaccaaggt cctgaccgat aggcaccgca
     2401 aggactacaa gacgctgatc caggagtacg cccagaagtg gcaccgttgc tacccggtct
     2461 atacgctcga gcggaaggcc ggaccggacc acgaccgcac ctattggata cgctgcgagt
     2521 tgctcggcgc gagctatggc ccggccgcgg gcaagaccaa gaaggacgcc gagc
//
LOCUS       DAOOLN010000143         2572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_49561_length_2572_cov_3.627334, whole genome shotgun sequence.
ACCESSION   DAOOLN010000143 DAOOLN010000000
VERSION     DAOOLN010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49561_length_2572_cov_3.627334"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="PLW80_02605"
     CDS             <1..1239
                     /locus_tag="PLW80_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65422.1"
                     /translation="MEFSSIEVSALDGGAEVLGFAVLPVFEGYARSGARVTMGHGVVA
                     GAEPGRYFVRFPLAQGDRSSLVASLAGDGAVSLMVIGPDGRERVAYTATVRGGDLLAV
                     PTAGFGAAAAVVVASGSTIASVQTEAGSGAPLSDLHAILSMPAPAGDYSIYRWDVLPR
                     TLVLDFADYDTQDRYLKRLAFFAEKPGFRGRLAADDEIAGLHGWNAHDYPTFTLARFY
                     ALAADTGFRLNAEEERFRALLLEHGILRLGDDGRPEEGEGAVISIARESSDELRRRFI
                     DHEASHALFFQDPEYRALATRLWNGLGPEARFFWKTHLAWRSYDVRDEYLCANELQAY
                     LTQQSLRAAPSYFMAITSRLAAAYPDRAERLTSEEAVVLASIRACGEELDAYLRRAWG
                     LSAGRFGRVASYYAGRSASR"
     gene            complement(1253..>2572)
                     /locus_tag="PLW80_02610"
     CDS             complement(1253..>2572)
                     /locus_tag="PLW80_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HPB65423.1"
                     /translation="LTHVLGDRRLRLLVDPEEAELNEVIAGCYVPAVTGDIVSVPLRG
                     RIDASPEPFHRAADAIRRSIDAISDDYSVQSFFGKRWFSNIVRNLELSNAPTPPVGPI
                     RSAYITAAGPSLERYAEEIRLRPKDAFLIATDTSLRALLGRGIRPDAVISIDCQQISY
                     YHFSLGLPPDVPLFLDLASPPTLSRLAVRPYFFSSGHPLSRYIASRYRAFPVLDTSGG
                     NVTHAAVSLAEYLGASTVRIFGADFSYPFGKSYARGTYIYPYFDVRQSRLRPSESLFA
                     EFLYRNQSISREDDDDGAFRYVTKPLVMYKERLERLAAVASCRIERERGFGVRAETLR
                     NVPPRGRDSGRVFAAGRPYQRPLEFLSAYHADIVALPAPYRHAAAYLDGLSPHEKDVW
                     TTMLPAAAVFGRLSGETQARPDELLTDTRDWCAGIVLNELRRSRDGA"
BASE COUNT          447 a          817 c          865 g          443 t
ORIGIN      
        1 atggaattct cttccatcga agtctcggcg ctcgatggcg gcgccgaggt cctgggcttc
       61 gcggtccttc ctgtattcga aggctacgcg cgctcgggcg cgcgtgtgac gatgggtcat
      121 ggcgtcgtcg caggcgcgga accgggacga tatttcgttc ggttccccct tgcccaaggt
      181 gatcgttcct cgctggtagc gagcctcgcc ggcgacggcg ccgtcagcct gatggttatc
      241 gggccggacg gacgggagcg agtcgcctat accgcgacgg tacgcggcgg cgacctgctg
      301 gccgtaccta cggccggctt cggagcggcg gcggccgtcg tcgtcgcgtc aggctcgacg
      361 atagcctcgg tccagaccga ggcgggcagt ggcgccccgt tgtccgattt gcacgccatt
      421 ctgtccatgc ccgccccggc cggcgattac tcgatctacc gctgggacgt actgcctcgc
      481 accctcgtgc tcgatttcgc cgattacgat acgcaggatc gatacctgaa gcgcctagcc
      541 ttcttcgccg agaagccggg cttcagggga cgcctggccg ccgacgacga gatagccggt
      601 ctccatggct ggaacgccca cgactatccg accttcacgc tagcgaggtt ctacgccctg
      661 gccgccgata ccggctttcg cctgaacgcg gaggaggaac gcttccgcgc cctgctactc
      721 gagcatggaa tcctgcggct cggcgacgac ggccgtcccg aggaaggcga gggcgcggtc
      781 atctccatag cccgcgagtc atcggacgag ctcagacgcc gcttcatcga ccacgaggcg
      841 tcgcacgcgc tgttcttcca ggatccagag taccgggcgc tggcgacgag gctctggaac
      901 gggctcgggc cggaggcgcg gttcttctgg aagactcacc tggcttggcg atcctacgac
      961 gtgcgcgatg agtacctctg cgccaacgag ctgcaggctt acttgaccca gcagagtctt
     1021 cgggccgccc cgtcgtactt catggccata acgtcgcgcc tcgcagcggc ctatccggat
     1081 cgcgccgagc ggctcaccag cgaggaggcg gtcgtgctcg catctatccg cgcctgcggc
     1141 gaggaactgg acgcgtacct gcgccgggct tgggggctgt cagccgggcg cttcggccgc
     1201 gtcgcgagct actacgcggg tcgctctgcg agtcgttaag cgtccatcca gctcaagccc
     1261 cgtcgcgcga gcgacgtagc tcattcagca caatgccggc acaccaatcc cgggtgtcgg
     1321 tcaagagctc gtcgggcctc gcctgggtct cgccggaaag gcgcccgaat acggccgcag
     1381 ccggcagcat cgtcgtccat acgtctttct cgtgcggaga cagcccgtcg aggtaggctg
     1441 cggcgtgacg gtatggcgct ggaagagcga cgatatccgc gtggtacgct gacaggaatt
     1501 cgagcggcct ctggtacggt cgacccgcgg cgaaaaccct gcccgagtcc cggccgcgag
     1561 gcggtacgtt gcgcaaggtc tcggcgcgca ccccgaaacc gcgctcccgc tctatcctgc
     1621 acgaggcgac ggccgctaga cgttccagcc tctccttgta catgaccagg ggcttcgtaa
     1681 cgtagcggaa agcgccgtca tcgtcgtcct ccctcgagat gctctggttg cggtacagaa
     1741 actcggcgaa gagcgactcg gacggccgta gcctcgactg ccgcacgtcg aaatacggat
     1801 agatgtaggt gcctcgcgcg tagctcttac cgaagggata ggagaagtca gccccgaaga
     1861 tcctgacggt cgacgccccg aggtactcgg cgagcgatac ggcagcgtga gtcacgttgc
     1921 cgcccgaggt gtcgagtacg gggaaggcgc ggtagcgaga ggctatgtat cgtgacaatg
     1981 ggtgaccgga ggagaaaaaa tacggccgaa cggccaggcg cgacagggtg ggcggcgagg
     2041 ccaggtcgag gaagagcggc acatccggcg gcaggccgag cgagaaatgg taatagctga
     2101 tctgctggca atcgatgctt atcaccgcgt cgggccgaat gccccgaccg agcaaggcgc
     2161 gcaggctcgt gtcggtggcg atcagaaacg cgtccttggg cctaagacgg atctcctcgg
     2221 cgtatcgttc gagagacggg ccggccgcag taatataggc cgagcgtatg ggcccgacag
     2281 gcggcgtcgg cgcgttggac agctcgaggt tacggacgat attcgagaac cagcgcttgc
     2341 cgaagaacga ttgaaccgag tagtcgtctg atatggcgtc tatggatcgg cgtatggcat
     2401 cggcggccct atggaacggc tcgggcgacg cgtctatcct gccgcgcagg ggaaccgaga
     2461 cgatgtcgcc ggtcaccgct ggcacgtaac acccggcgat aacctcgttc agttcggcct
     2521 cctccggatc gacgaggagc ctgagccttc gatcgccgag cacatgggtt ag
//
LOCUS       DAOOLN010000144         2566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_49737_length_2566_cov_2.864596, whole genome shotgun sequence.
ACCESSION   DAOOLN010000144 DAOOLN010000000
VERSION     DAOOLN010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49737_length_2566_cov_2.864596"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1320
                     /locus_tag="PLW80_02615"
     CDS             <1..>1320
                     /locus_tag="PLW80_02615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HPB65424.1"
                     /translation="DYGAGPGAPEYRYASRIGAMPPPGHQYRRLSYPRILDFSGRSWF
                     VEVRASTGYVSGVSSDAGPEFFLFAGALASFVLGFAVHSLSRSRARAEASAAAAGEEA
                     ELFFQLGPDMFMITELDGRIRRLNSEWTASLGYRVGELEGYLLIDFIHPDDREESIRV
                     CRGVGDGIALEGYVNRVRTAAGDYRRVEWRARRAPGGGAVFVGARDVTERESMEERLR
                     GSLVEREALIKEVHHRVKNNMQVISSLLELGSMDEADARFTAVAKEARGRIRSMALVH
                     EQLYKRDSISNIDLGEYLRELAGRVVGEFASGAVDLVVDAGVVPLDIDAAIPCGLVVN
                     ELVTNALKYAGAGGRRASLRISAREEGGWCSVAVEDDGPGIPDGAFERSMAGATLGLS
                     LVSGLASQLRGELRASSGPGARVELRFPLAPRGAGPGLADSPSDRPVG"
     assembly_gap    1321..1420
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1447..2067)
                     /gene="udk"
                     /locus_tag="PLW80_02620"
     CDS             complement(1447..2067)
                     /gene="udk"
                     /locus_tag="PLW80_02620"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0046108 - uridine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HPB65425.1"
                     /translation="MAEAKLIGICGGSGSGKTTIVRKISELVADFVFIPQDNYYKSAE
                     YVSNSNITAFNFDHPEAFDNELLWSQLSALKRMEPVEIPTYDFVHHRRAEETIKVTPR
                     KLVIFEGIMVFTNEKVRDLIDLKIFVDTPDDIRFIRRLSRDIKERGRTVDSVIDQYLN
                     VVRPGHFQFIEPTKRFADIIIPEGGANENALEVLVTFIKTILNSEG"
     gene            2295..>2566
                     /locus_tag="PLW80_02625"
     CDS             2295..>2566
                     /locus_tag="PLW80_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65426.1"
                     /translation="MKRRVPLIVACVLAAILVSSCLPGDGRNTSANPAGFLSGFWHGI
                     VAPVSLVVQIFKPTIRVYEVANTGFWYDLGFWLAIGGGAGGGCAAVG"
BASE COUNT          342 a          856 c          825 g          443 t
ORIGIN      
        1 gactacgggg ccggccccgg cgcccctgag taccgctacg cgtcgcggat cggcgccatg
       61 ccgcccccgg ggcaccagta ccgccgccta tcgtacccca ggatactcga cttctccggg
      121 cgatcctggt tcgtcgaggt ccgggcatcc accggctacg tatccggggt ctcctcggac
      181 gccggcccgg agttcttcct cttcgcgggc gccctggcct ccttcgtgct cggcttcgcc
      241 gtccactccc tgtcccggtc cagggcccgg gccgaggcca gtgcggccgc cgccggcgag
      301 gaagccgagc tgttcttcca gctcggcccc gacatgttca tgataaccga gttggacggc
      361 cggatacggc ggctcaactc ggagtggacg gcctccctgg gctaccgggt cggcgagctg
      421 gagggctacc tcctcatcga tttcatccat cccgatgacc gggaggaatc gatccgcgtg
      481 tgccgtggcg tcggcgacgg catcgccctc gagggctacg tcaaccgggt caggaccgcc
      541 gccggcgatt accgacgcgt cgagtggcgg gccaggcgcg cgcccggtgg cggcgccgtc
      601 ttcgtcggcg ccagggacgt gaccgagcgc gagtcgatgg aggagcggct gcgcggctcg
      661 ctcgtcgagc gagaggcgct cattaaggaa gtgcatcacc gcgtcaagaa caacatgcag
      721 gtcatctcga gcctgctcga gctcggctcg atggacgagg ccgacgcgcg tttcacggcc
      781 gtggccaagg aggcccgggg ccgcatccgc tccatggccc tggtccacga gcagctgtac
      841 aagcgcgact cgatctcgaa tatcgacctc ggggagtacc tgcgcgagct ggccggccgc
      901 gtggtcggcg agttcgcctc gggcgccgtc gacctcgtgg tggacgccgg ggtcgtaccg
      961 ctcgacatcg acgcggccat accctgcggg ctggtggtca acgagctcgt gaccaacgcc
     1021 ctcaagtacg ccggcgcggg cggacgccgc gcctccctcc gtatcagcgc gcgtgaggaa
     1081 ggcggctggt gctccgtcgc ggtcgaggac gacggccccg gcattcccga cggcgctttc
     1141 gagcgctcga tggccggcgc cacgctcggc ctgagcctgg tatcgggcct ggcctcccag
     1201 ctacgcggcg agctacgcgc gtcgtccggc ccgggcgccc gtgtcgagct gcgcttcccg
     1261 ctggcgcctc gcggcgctgg gccgggcttg gcggacagcc cgtcggaccg cccggtgggc
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccgcccggtg ggcgctccct
     1441 cgggcgtcag ccctcggagt tgagtatcgt cttgatgaag gtaacgagca cctccagcgc
     1501 gttctcgttc gctccgccct cgggtatgat gatgtcggcg aagcgcttgg tcggctcgat
     1561 gaactggaag tgtcccggcc gtaccacgtt caggtactgg tcgatcaccg agtcgaccgt
     1621 gcgaccgcgt tccttgatat cccgcgacag ccttcggatg aaccgaatat cgtccggcgt
     1681 gtccacgaag atcttcaggt cgatcaggtc gcgcaccttc tcgttcgtga acaccatgat
     1741 gccctcgaag atcaccagtt tgcgcggcgt gaccttgatc gtctcctccg ccctgcggtg
     1801 gtgcacgaag tcgtaggtcg gtatctcgac cggttccatc cgtttgagcg ccgagagctg
     1861 gctccagagc agctcgttgt cgaacgcctc cgggtggtcg aaattgaagg cggtgatgtt
     1921 cgagttcgag acgtactccg ccgacttgta gtagttgtcc tggggaatga acacgaagtc
     1981 ggccacgagc tcggatatct tgcgcacgat cgtcgtcttg ccggatcccg agccgccgca
     2041 gatccctata agtttcgctt cagccatggc gcgaggatag cacgctcctc gcgccttggg
     2101 aacggggggc gccatcgtcc gcctcccggt cgccgcggcc ggacgcgccc cccggcccga
     2161 gcctcctcgc gcgtcccgcg gccgcgctcc ggggtctcgg gcctcccccg ggacctccat
     2221 aggcaccgag ccttgcgggc ggcctctcgg cgcgctatat aggtgtagac tccgtttcga
     2281 tgaaaggata cccaatgaaa cgtcgcgttc cgctgatcgt cgcctgcgtt ctagccgcta
     2341 tccttgtatc gtcctgcctg cccggggacg gtaggaacac gagcgccaat cctgccggct
     2401 tcctctcggg cttctggcac ggcatcgtcg cgcccgtgtc gctcgtcgtg cagatcttca
     2461 agccgaccat ccgcgtctac gaggtagcca acacgggctt ctggtacgac ctgggcttct
     2521 ggctcgccat cgggggcggc gccggaggcg gatgcgccgc ggtggg
//
LOCUS       DAOOLN010000145         2566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_49740_length_2566_cov_2.753883, whole genome shotgun sequence.
ACCESSION   DAOOLN010000145 DAOOLN010000000
VERSION     DAOOLN010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49740_length_2566_cov_2.753883"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLW80_02630"
     CDS             complement(<1..212)
                     /locus_tag="PLW80_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65427.1"
                     /translation="MPYCSRCGVEVDDATSDCPLCEAPIQRLDAPASTLGAGPYPQHI
                     IDPQDRYRLSRAERRRIAIEILSLVA"
     gene            complement(213..1625)
                     /locus_tag="PLW80_02635"
     CDS             complement(213..1625)
                     /locus_tag="PLW80_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65428.1"
                     /translation="MKTEGDADAPQPWQAPIRSKFCRKYTDDRRYPDDRRYPDDHPWF
                     ALDHAAIIYPSILSDRVSAYYRIQAILDEPVDIALVQRALDALRPRFPYFQIELRRGF
                     FWFFFERNDSPNLVVEDSKFPMQRLSSGRKGVYLYRVRAHADRLSLEMCHILTDGYGA
                     MIYFRSLLAEYFRLKGVTTEYGPMVFAPNDAPGPEEWEDAFSRYATPGAPGPAKLGPA
                     WHLPGIGLPVHTMRVVTGELSLSSALAKAKEHGATLTIYLSSVFLAALQDVQDAEYEG
                     RPRSRRRPLRLQVPANLRNFFPSSTLRNFSLYALPELDTRLGHYEFSEIVARVKATMA
                     LAFTPKEIMRTIMRNVATAKHPVIRAVPLTMKNLIMRGLYRSHGEALYSSLSTNVGQA
                     RLPDSFASKVKRIDVVLPSTPGLKTVAGMLSHGDALSISFGSVIERRDLERAFFTRLV
                     RDGLKVRVESNLGAPEGKDA"
     gene            1715..>2566
                     /locus_tag="PLW80_02640"
     CDS             1715..>2566
                     /locus_tag="PLW80_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPB65429.1"
                     /translation="MIDLHTHSTASDGALSPAALIGKAAEEGLGAIALTDHDCLDGLD
                     EARTAASSIGMRFIAGIEIEIAFEPGEFHLLGLDLFDTRRPLRDAAASLADSRERRNR
                     AVLELFRDDGVELDYDELRAKAGTGMIGRPHIASMLVEKRIVRSKQAAFDKYLAKGRP
                     YYVRKACLELPEALRVVKDSGGLAFVAHPMSLFASWKRLRALFEEWKALGVDGIEAWH
                     PTARLVDCQRLDAMAREFGFRVSAADTLSPERQNELLQSALTAFDQAVAVSRESPQKA
                     SELYRQAA"
BASE COUNT          441 a          822 c          863 g          440 t
ORIGIN      
        1 atggccacca ggctgagtat ctcgatcgcg atgcgccgac gctcggccct ggacagccgg
       61 tacctgtcct gcgggtcgat gatgtgctgg gggtagggac cggcacccag cgttgaagcc
      121 ggcgcatcca ggcgctggat cggcgcctcg cagagcgggc aatcgctcgt cgcgtcgtcg
      181 acctcgacgc cgcatctact gcaataaggc atctatgcgt cctttccttc gggcgcgcca
      241 aggttcgact cgacccgtac cttgagcccg tccctgacga gccgcgtgaa aaaggccctc
      301 tcgaggtcgc gtcgctcgat cacgctaccg aagctgatcg acagcgcgtc gccgtggctg
      361 agcatgccgg cgaccgtctt gaggcccggg gtcgacggca gcaccacgtc gatgcgtttg
      421 accttggacg cgaaggagtc gggtagcctg gcctggccga cgttagtcga caggctcgag
      481 tagagggcct cgccgtggct gcggtagagg ccgcgcatga tcaggttctt catcgtcaac
      541 ggcacggccc ggatgacggg atgtttggcc gtcgccacat tccgcatgat cgtccgcatg
      601 atctccttgg gcgtgaaggc gagcgccatc gtcgccttga ccctcgcgac gatctcggag
      661 aattcgtaat ggccgagcct cgtgtccagc tccggcagcg cgtagaggct gaagttgcgc
      721 agggtcgacg aggggaagaa gttcctcagg ttggccggta cctggaggcg caggggccgc
      781 ctgcgcgacc gcggccgccc ttcgtactcg gcgtcctgga cgtcctgcag cgcggcgagg
      841 aacaccgacg ataggtagat cgtcagggtg gcgccgtgtt ccttggcctt cgccagggcc
      901 gatgacagcg acagctctcc cgtgacgacg cgcatcgtat ggacgggcag gcctatgccc
      961 ggcaggtgcc aagccggacc tagcttggcg gggcccggcg ccccgggcgt cgcgtagcgg
     1021 gagaacgcgt cctcccattc ttcaggcccc ggcgcgtcgt tcggggcgaa caccatgggc
     1081 ccgtactcgg tcgtaacgcc cttgagcctg aagtactcgg ccaggagaga cctgaaatag
     1141 atcatcgctc cgtagccgtc ggtcaggata tggcacatct cgagcgacag gcggtcggcg
     1201 tgggcgcgca cgcggtacag gtagacgcct ttccgtccgg acgacaggcg ttgcatcggg
     1261 aatttcgagt cctcgaccac gaggttcggc gagtcgttgc gctcgaagaa gaaccagaag
     1321 aagcctcgcc tcagctcgat ctggaaatag gggaaccgcg gcctgagcgc gtccagtgct
     1381 cgctggacga gggcgatgtc tactggctcg tcgagtatgg cctggatgcg gtagtaggcg
     1441 ctgacgcggt cggacaggat ggacgggtag atgatggccg cgtgatcgag cgcgaaccag
     1501 ggatggtcgt cggggtaccg ccggtcgtcg gggtaccgcc ggtcgtcggt gtacttacgg
     1561 cagaacttgg agcgaatggg agcctgccat ggttgcggcg catccgcgtc gccttcggtt
     1621 ttcacggcta ttataatact gacaagtcgt cgctttgccc atgccgagcg cgggcgctat
     1681 gcaaaaaacg atgaaagcgg cagaatagga tcagatgata gatctgcata cgcattctac
     1741 ggcgagcgac ggcgccctat ccccggcggc cctcatcggc aaggccgccg aggaaggcct
     1801 cggcgccatc gcgctgaccg atcacgactg cctggacggc ttggacgagg cgcggaccgc
     1861 cgcctcgtcc atcggcatgc gtttcatagc gggcatcgag atagagatag cgttcgagcc
     1921 cggcgagttc cacctgctcg gcctggacct gttcgataca cgtcggcccc tgcgcgacgc
     1981 ggccgcctcg ctggcggact cgcgcgaacg gcgtaaccgg gcggtcctgg agctgtttcg
     2041 cgacgacggc gtcgagctcg actacgacga gctacgcgcc aaggccggca ccggcatgat
     2101 tggccgtccc catatagcct cgatgctggt ggagaagcgc atcgtgcgta gcaagcaggc
     2161 cgccttcgac aagtacctgg ccaaggggcg gccttactac gtgcgcaagg cctgcctcga
     2221 gctgcccgag gcgctacgcg tcgtcaagga ctcgggaggc ttggccttcg tcgcccatcc
     2281 aatgtccctg ttcgcgagct ggaagcgcct gcgcgccctg ttcgaggaat ggaaggccct
     2341 cggcgtcgac ggcatcgagg cctggcaccc aaccgcccgc ctcgtcgact gccagcgcct
     2401 cgacgcgatg gcccgcgagt tcggcttccg cgtcagcgcc gccgatacgc tctcgcccga
     2461 gcgacaaaac gaactgctcc aatccgctct caccgcgttc gaccaggccg tcgccgtctc
     2521 gcgcgaaagc ccgcaaaaag ccagcgagtt gtaccgccag gcagcg
//
LOCUS       DAOOLN010000146         2558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_49961_length_2558_cov_2.780663, whole genome shotgun sequence.
ACCESSION   DAOOLN010000146 DAOOLN010000000
VERSION     DAOOLN010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49961_length_2558_cov_2.780663"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="PLW80_02645"
     CDS             complement(<1..965)
                     /locus_tag="PLW80_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPB65430.1"
                     /translation="MLEHIVRTKPAIQAAVKERLAAYAAEYAAVSGDFGASTAARLDE
                     FSSRGKMLRGCLVPFGYELAAGRVPAGDDGRATILAGAAMELFQSGLLVHDDIMDRDR
                     IRRGGPTVHAAYEAELERGRYADPGHNGQSLGICSGDVAFFAAFRILSELPVGAEAAR
                     RVVAIAARELSLVGVAQMQDVANGAIRPGSENPFRDAPCEPDEAGVLALYRYKTGRYT
                     FSLPLALGAAIAGADEQTSQALERAGESLGVLFQLKDDELGLFADEYELGKPIGADVR
                     EDKKTLLRLYLFALASGDERARLESMFGNPSAGAAEIEYLRSMAIG"
     regulatory      1061..1142
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            1161..2435
                     /locus_tag="PLW80_02650"
     CDS             1161..2435
                     /locus_tag="PLW80_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPB65431.1"
                     /translation="MALSLAEIIILCLLADWAFKAFNIPGLIGMLAAGAVLGPAVLDL
                     IDPALLSIGADLRMIALIVILLRAGFELSRNTLHRVGKQALLLAFLPALFEALAVTAL
                     GPRLLGLSIMESAILGCVLGAVSPAVVVPMMVRFNQERRGVAKGIPTLVLAGASIDDV
                     TVIVAYSVLVGIYTGGRVNVAWKILGIPLSIISGIAVGLALGLALYLLFERFNPRATK
                     RALVVIGASILLVQAGDRLEALDVPFAALIAVMAIGFVILEKKESMAHELSAKLGKIW
                     VFAEIILFSMVGAQVNFRAAADAGLGGVALILIALVSRSIGTLLCTAGAGLTTREKLF
                     VVISYLPKATVQAAIGSAPLAAMAAAGMPTAPGETILAVAVMSIALTAPIGAWAIAWA
                     GRNLLAVDEQGSGDSDRVAAYESDALVDDEDR"
BASE COUNT          378 a          894 c          845 g          441 t
ORIGIN      
        1 ccgatcgcca tcgagcgcag gtactcgatt tcggccgcgc cggcggacgg gttcccgaac
       61 atggactcga gccgggcgcg ttcgtcgccg gaagcgagcg cgaacaggta caggcgcagg
      121 agcgtcttct tgtcctcccg aacgtcggcg ccgatgggct ttccgagttc gtactcatcg
      181 gcgaacaggc ccagttcgtc gtccttgagc tggaatagga cgccgaggct ctcgccggcg
      241 cgctcgagcg cctggctcgt ctgctcgtcg gctccggcaa tggccgcgcc gagggccagc
      301 ggcaggctga aggtatagcg gccggtcttg tagcggtata gcgcgagcac gccggcctcg
      361 tccggctcgc agggcgcgtc gcggaacggg ttctccgagc cgggcctgat ggcgccgttg
      421 gccacgtcct gcatctgcgc cacgccgacg agggacagct ccctggcggc tatggcgacg
      481 acgcgccgcg ccgcctccgc gccgacgggc agctccgaga gtatcctgaa cgcggcgaag
      541 aacgccacgt ctcccgagca gatgccgagc gactggccgt tatggcccgg gtcggcgtag
      601 cggccccgct cgagctcggc ctcgtaggca gcatgcacgg tcggcccgcc gcgacggatc
      661 cggtcccggt ccatgatgtc gtcatggacg agcagaccgg actgaaacag ctccatggcg
      721 gcgccggcga gtatcgtcgc gcgcccgtcg tcgccggccg gcacccggcc ggcggccagc
      781 tcgtagccga agggcacgag gcaaccgcgc agcatcttgc cgcgcgagga gaattcgtcg
      841 agcctggcgg ccgtcgacgc gccgaagtcc cctgagactg cggcgtactc ggccgcatag
      901 gcggccaggc gttccttgac ggcggcctgg atagcgggct tcgtcctgac tatatgctcg
      961 agcactggac tctcctcggt tcgccagtct agccccgcgc ttcggcgggg tcaagcgagc
     1021 ccgtgcccga tattgacgaa cgattacggc atcgttaccg tgtgcgcgtg gcgatggagt
     1081 tcgccctggc cctcggtcag aaccgtacgc cgaccggcgg taccgatgac tcctaccgaa
     1141 cccccatcga tggagtatcc atggccctaa gcctcgctga aatcatcatc ctgtgcctgc
     1201 ttgccgattg ggcgttcaag gccttcaata tccccggcct gatcggcatg ctcgccgccg
     1261 gcgccgtgct cggaccagcc gtcctggatc tgatcgatcc agcgctccta tcgatcggcg
     1321 ccgacctcag gatgatagcg ctgatcgtca tcctcctgcg agccggcttc gagctgtcca
     1381 ggaataccct gcaccgcgtc gggaagcagg ccttgctcct ggcgttcctg cccgccctgt
     1441 tcgaggccct ggccgtcacc gcgctcgggc cgcgcctgct cgggctgtcg atcatggagt
     1501 cggccatact cggctgcgtg ctgggggccg tatcaccggc cgtcgtcgta ccgatgatgg
     1561 tccgcttcaa ccaggaacgc cgcggcgtgg ccaagggtat cccgaccctc gtactggccg
     1621 gggcgagcat cgacgacgtg acggtcatcg tcgcgtatag cgtcctcgtc ggcatctata
     1681 ccggaggcag ggtcaacgtt gcctggaaga tcctcggcat accgctttcg atcatctccg
     1741 gtatcgccgt cggattggcc ctcggcctcg cgttatacct gctcttcgaa cgcttcaacc
     1801 cgcgggcgac aaagcgcgcc ctcgtcgtga tcggcgcgtc catcctgctc gtccaggccg
     1861 gcgaccgact ggaggcgctc gacgtgccct tcgccgcatt gatcgccgtg atggccatag
     1921 gcttcgtcat cctggagaag aaggagagca tggcccacga gctgtcggcc aagctcggca
     1981 agatatgggt cttcgccgag atcatcctgt tctcgatggt cggcgcccag gtgaacttca
     2041 gggccgccgc cgacgccggc ctcggcggcg tcgccctcat cctgatagcc ctcgtgtcca
     2101 ggtccatcgg aaccctgcta tgcacggcgg gggccggcct gacgacgcgc gagaagctat
     2161 tcgtcgtcat cagctacctg ccgaaagcca cggtccaggc cgccatcggt tctgccccgc
     2221 tcgccgcgat ggcggccgct ggcatgccga cggcgccggg cgagaccata ctcgccgtcg
     2281 ccgtcatgag catcgcgctg acggccccga tcggggcctg ggcgatagcc tgggccggcc
     2341 gaaacctgct ggccgtggac gagcagggct caggcgactc ggaccgcgtc gccgcctacg
     2401 agagcgacgc cctcgtcgac gacgaggacc gttagccctt cgcggcgagc gcctccctcg
     2461 gggccttcat cgcgtcggtc cagtaagcca gctggtggag catggcctgg gcggccttct
     2521 tctggccctc gtccggtacg aaggagccgc tcgagtcg
//
LOCUS       DAOOLN010000147         2550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_50188_length_2550_cov_2.729860, whole genome shotgun sequence.
ACCESSION   DAOOLN010000147 DAOOLN010000000
VERSION     DAOOLN010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50188_length_2550_cov_2.729860"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="PLW80_02655"
     CDS             <1..1365
                     /locus_tag="PLW80_02655"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65432.1"
                     /translation="VRSTWRRKSWPSPTPADAGEEPDLRLSFDEAHPSDPSGKWSLEV
                     VGPVSISPSSRFGAGAGSFRAPATRLVLQPASVGVFTPNMPIGDMSLEFWLKPTRADS
                     GEIVMLWKANRKAGSSWLPQQVTCLISRNRMTFGFINFFADPSGKATTLSLQGSSVVV
                     PAAWSHHLVRFDSSTGLVEYLMNGEIEAVAYATSTGKQAGAVWSPMAGDSGRLEVAPN
                     YTGLIDELRIVRTFISAPRLGRYGAAGGTAVSPIFDLGGTNSLLKSIEADAMTPAESA
                     VHWSYRIGDSSAGWGDDEPAWVPFSPGVALSRAGAEIRGRYLQLRMDLYPDASGELSP
                     TVSTIRARFEPDLPPSPPAMARAKAGDVSIAVSWSPLTEADIAGYVVYYGSAPGDYFG
                     ADAREGRSPIFVPGGSASGLTLTGLKNGSLYFLAVAAYDGAHPPHIGDSSREISARPS
                     RVSP"
     gene            1362..>2550
                     /locus_tag="PLW80_02660"
     CDS             1362..>2550
                     /locus_tag="PLW80_02660"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB65433.1"
                     /translation="MSTAKKKTDSLALAQNALRLRDFETAERYSRKHLLADPKSSEAR
                     MVLGTVYLRTDRASDALAAFSSAVEADASDLRALEGMAVSYLRLGKPLDALAALERAI
                     DLNPNDAELLFNLGNAHKALGNLRAAEMSFAKAIELDGSHVMAYNNLGNVYLSLGDAS
                     KAVQVYWRGLAIDPNYPSFHYNLGMAYEKLGKPSDAITEYAAAVKGRPGWIDAMTRYG
                     ECLARNAESKRAIDVFTQIIAIDPLNAQARNGIGLAYAEAGKPDEAVSWFRKALEADP
                     RFLLAAVNIQDVLEKTGRYAEAVGEIQALVDLMPDNDGLRVQLARLYKALGHLDLAMD
                     TITDVLRRSPNDIEALKLYGTLLQALGKPAKAKEVYKKIVKLDPEQASFRIELARLYR
                     EAGD"
BASE COUNT          443 a          901 c          802 g          404 t
ORIGIN      
        1 gtgcgctcca cgtggcgcag gaagtcgtgg cccagcccga cgccggccga cgcgggggag
       61 gagcccgacc tgcggctgtc cttcgacgag gcgcatccat ccgatccgtc gggcaaatgg
      121 tctctcgagg tcgtcgggcc ggtgtcgatc agcccgtcgt cgcgtttcgg ggccggggcc
      181 ggttcgttca gggcgcccgc gaccaggctc gtgctccagc cggcgtccgt cggcgtcttc
      241 actccgaaca tgccgatcgg cgacatgtcc ctcgagttct ggctgaagcc gaccagggcg
      301 gactccggcg agatagtcat gctctggaag gccaaccgca aggccggttc ctcctggctg
      361 ccgcagcagg tcacgtgcct gatatccagg aaccgcatga ccttcggttt catcaacttc
      421 ttcgccgatc cgagcggtaa ggcgacgacg ctcagcctac agggctcgtc ggtcgtcgtc
      481 ccagccgcct ggtcgcatca cctcgtacgc ttcgactcct ccaccgggct ggtcgagtac
      541 ctgatgaacg gcgagatcga ggcggtggcc tacgccacct cgaccggtaa gcaggccggc
      601 gcggtctgga gccccatggc gggagactcc gggcgcctgg aggtcgctcc taactatacg
      661 ggcctgatcg atgagctgcg gatcgtgcgg accttcatca gcgcacctcg cctcgggcga
      721 tatggcgctg caggcggtac ggccgtctcg ccgatattcg atctcggcgg caccaactcg
      781 ctactgaagt ccatagaggc cgacgccatg acgccggccg agtcggctgt ccactggagt
      841 taccgcatcg gcgactcctc ggccggttgg ggcgacgacg aaccggcctg ggtaccgttc
      901 tcgcccggag ttgccctgtc acgagccggc gccgagatcc gcggccgcta cctgcagctc
      961 aggatggacc tatatcccga cgcctcgggc gagctgtcgc cgaccgtctc gacgatacgc
     1021 gcccgcttcg agcctgacct gccgccgagc ccgccggcca tggcgagagc caaggccggc
     1081 gacgtctcca tcgccgtttc ctggtctccc ttgaccgagg cggacatagc cggatacgtg
     1141 gtatactatg gctccgcgcc aggcgactac ttcggcgctg acgcccgcga gggacgtagc
     1201 ccgatctttg tgcccggcgg tagcgcttcg ggcctgacgc tgaccggcct caagaacggg
     1261 tcactatatt ttttggccgt cgccgcgtac gatggcgctc atcctcccca tatcggggat
     1321 tcatcgcgcg agatcagcgc gcggccatcg agggtctcgc catgagcaca gcgaagaaaa
     1381 aaaccgacag cctcgccctc gcccagaacg cgctcaggct acgcgacttc gagacggcgg
     1441 aacgatactc gcgcaagcac ctactcgccg acccgaaatc ctcggaggcc cgcatggtgc
     1501 tcggcacggt gtacctacgc accgaccggg cgagcgacgc gctcgccgcg ttctccagcg
     1561 cggtcgaggc cgacgcctcc gacctacgcg cgctcgaggg catggccgtg tcgtacctgc
     1621 gcctcggcaa gcccctggac gccctggccg cgctcgagcg ggccatagac ctgaacccga
     1681 acgacgccga gctactgttc aacctcggta acgcccacaa ggccctcggc aacctccgcg
     1741 ccgccgagat gtcgttcgcc aaggccatcg aactggacgg cagccacgtg atggcctaca
     1801 acaacctcgg caacgtctac ctatcgctcg gggacgcctc caaggccgtc caggtctact
     1861 ggcgcggcct ggcgatcgac ccgaactacc cgtcgttcca ctacaacctc ggcatggcct
     1921 acgagaagct ggggaagccg tccgacgcga tcaccgagta cgcggccgcc gtcaaggggc
     1981 gacccggctg gatcgacgcg atgacgcgat acggcgagtg cctggctagg aacgccgagt
     2041 cgaagcgcgc catcgacgtg tttacccaga tcatcgccat agacccgctc aacgcccagg
     2101 ccaggaacgg catcgggctc gcctacgccg aggccggcaa gccggacgag gccgtctcct
     2161 ggttccgcaa ggccctcgag gccgatcccc gcttcctact cgccgcggtg aacatccagg
     2221 acgtgctcga gaagaccggc cgctacgccg aggcggtggg ggaaatccag gccctcgtcg
     2281 acctgatgcc cgataacgac gggcttcgcg tccagctggc gcgcctgtac aaagcgctcg
     2341 ggcatctcga cctggccatg gatacgatca ccgacgtgct tcgccggtcg cccaacgaca
     2401 tcgaagcgct caaactctat ggcaccctgc tccaggcact cggcaagcct gccaaggcca
     2461 aggaagtcta caagaaaatc gtcaagctcg acccggagca ggcgtcgttc aggatcgagc
     2521 tggccaggct gtaccgggag gcgggcgatc
//
LOCUS       DAOOLN010000148         2549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_50227_length_2549_cov_2.500401, whole genome shotgun sequence.
ACCESSION   DAOOLN010000148 DAOOLN010000000
VERSION     DAOOLN010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50227_length_2549_cov_2.500401"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PLW80_02665"
     CDS             <1..635
                     /locus_tag="PLW80_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPB65434.1"
                     /translation="TGIKLARPELKVIVMTGDGDCTAIGGNHFIHAARRNVDITTIVM
                     NNNNYGMTSGQYSPMTPTGMLGTTAPYGNVERPFDLANLAIASGATFVARGTSYHVQL
                     LGEMIEKALLNPGFSVVEAVVGCPTYYGRKNKIGGAVELFNWQKEHAVNVKAAAAMAP
                     EKLEGKFLIGELYNKAAPEFTAEYARLVEGVRRKFDDFQGGVRGCAQNSD"
     gene            614..1150
                     /locus_tag="PLW80_02670"
     CDS             614..1150
                     /locus_tag="PLW80_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPB65435.1"
                     /translation="MRTEFRLSGSGGQGLLLAGIELAEGAILDGKNAVQTQSYGPEAR
                     GGSSKAEVVVCEDDIDYPKATDPDFLLALTTDAYKAYGTLMGKGLIIVDSSVCLDPAI
                     AARTVAVPILDTAATTIGKKVVANIVALGVLGGVSGVVRQATLEKAVLNRVPKGTEDL
                     NLKALRAGFELAAKATKA"
     gene            1164..>2549
                     /locus_tag="PLW80_02675"
     CDS             1164..>2549
                     /locus_tag="PLW80_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPB65436.1"
                     /translation="MSDTDSIKKGFEAYDSGVEKSIARFPERKKSFVTGSKSPVKRLY
                     TPLDDAGRDYARDVGFPGEYPYTRGVQNTMYRGRFWTMRQYAGFSTAEESNKRYKFLL
                     EQGTTGLSVAFDLPTQIGYDSDHALSQGEVGKVGVAIDSLADMEILFDGIPLDKVSTS
                     MTINAPASVLLAMYIAVAEKQGVSPDKLEGTIQNDILKEYVARGTYIFPPAPSMRLIT
                     DIFAYCSKSVPNWNTISISGYHIREAGSTAAQEVAFTIADGIAYVDAAIKAGLDVDQF
                     APRLSFFFNAHNDLFEEVAKFRAARRVWAKVMKERFGAKSPKSLSLRFHTQTAGSTLT
                     AQQPDNNIVRVAIQTLAAVLGGTQSLHTNSKDEALALPTEASVRIALRTQQIVAYESG
                     AAETIDPLAGSYYVESMTDRIQKEAEAYIATIDGLGGAVKAIEKGYPQQEIQDAAYQY
                     QMDVESGERVVV"
BASE COUNT          499 a          877 c          790 g          383 t
ORIGIN      
        1 ccaccggtat caagttggcg cgccccgagc tcaaggtcat cgtgatgacc ggtgacggcg
       61 actgcaccgc catcggcggc aaccatttca tccacgcggc ccgacgcaac gtcgacatca
      121 cgaccatcgt gatgaataat aacaactacg gcatgacgag tggccagtac tcgccgatga
      181 cgccgaccgg catgctcggc acgacggcgc cctacggcaa cgtggagcgg cccttcgacc
      241 tggccaacct ggccatagcc tcgggcgcca ccttcgtcgc ccgtggcacc tcctaccacg
      301 tacagctgct cggcgagatg atcgagaagg ccctgctcaa cccgggcttc tccgtggtcg
      361 aggcggtcgt cggttgcccg acctactacg gccgcaagaa taagatcggc ggcgcggtcg
      421 agctgttcaa ctggcagaag gagcacgcgg tcaacgtaaa ggccgcagcc gcgatggcgc
      481 ccgagaagct cgagggcaag ttcctgatcg gcgagctgta caacaaggcg gcgccggagt
      541 tcaccgccga atacgccagg ctcgtcgagg gcgtgcggcg taagttcgat gatttccagg
      601 ggggagtacg gggatgcgca cagaattcag actaagcggc tcgggaggcc aaggcctcct
      661 gctcgccggc atcgagctcg ccgagggcgc catcctggac ggcaagaacg ccgtccagac
      721 ccagagctat gggcccgagg cccgaggcgg ctccagcaag gccgaggtcg tcgtctgcga
      781 ggacgatata gactatccca aggccaccga cccggacttc ctgctcgcgc tgacgaccga
      841 tgcttacaag gcctacggca cgctgatggg caaaggcctg atcatcgtcg attcgtcggt
      901 atgcctcgac ccggccatcg ccgcgcggac cgtcgccgtg ccgatcctcg acaccgcggc
      961 gacgacgata ggcaagaagg tcgtcgcgaa catcgtcgcc ctcggcgtgc tcggaggcgt
     1021 atccggcgtc gtcaggcagg ccactctcga gaaggcggtg ctcaaccgcg ttccgaaggg
     1081 taccgaggac ctgaacctca aggcgctgcg ggccggcttc gagctcgcag ccaaggccac
     1141 gaaagcctaa ggcggaggca agcatgagcg ataccgattc catcaagaag ggcttcgagg
     1201 cctacgactc gggcgtcgag aagtcgatag ccaggttccc cgagcgcaag aagtccttcg
     1261 tgaccggctc gaagtcgccg gtcaagcgtc tgtacacgcc gctcgacgac gcgggccgtg
     1321 actacgctcg cgacgtcggc ttccccggcg agtatccgta cacgcgcggc gtccagaaca
     1381 ccatgtaccg cggccgcttc tggaccatgc gccagtacgc cggcttctcc accgccgagg
     1441 agtcgaacaa gcgctataag ttcctgctcg agcagggcac cacgggcctg tcggtcgcct
     1501 tcgacctgcc gacccagatc ggctacgatt ccgaccacgc gctgtcccag ggcgaggtcg
     1561 gcaaggtggg cgtagccatc gactccctgg ccgacatgga aatcctgttc gacggcatac
     1621 cgctcgacaa ggtgtccacc tcgatgacga tcaacgcccc cgcctcggtg ctcctggcga
     1681 tgtatatcgc cgtggccgag aagcagggcg tcagcccgga caagctcgaa ggtacgatcc
     1741 agaacgacat actcaaggag tatgtcgccc gcggcaccta tatcttcccg ccggcgccgt
     1801 cgatgcggct catcaccgac atcttcgcgt actgctccaa gagcgtgccc aactggaaca
     1861 cgatctcgat ctccggctac catatccgcg aggccggctc gacggccgcc caggaggtcg
     1921 ccttcacgat cgccgacggc atcgcctacg tcgacgccgc gatcaaggcc ggactcgacg
     1981 tggatcagtt cgcgccgcgc ctgtcgttct tcttcaacgc ccacaacgac ctcttcgagg
     2041 aagtggccaa gttccgggcc gcccgccgcg tctgggccaa ggtgatgaag gagcgcttcg
     2101 gcgccaagag cccgaagagc ctgtcgctac gcttccatac gcagaccgcc ggctccacgc
     2161 tgacggccca gcagcccgat aacaacatcg tccgcgtggc gatccagacc ctggccgccg
     2221 tgctcggcgg cacccagtcc ctgcacacca actccaagga cgaggctctc gcgctcccga
     2281 ccgaggcctc ggtgcgcatc gcgctgcgta cccagcagat cgtcgcctac gagtcgggcg
     2341 ccgcggagac catcgacccg ctcgccggct cgtactacgt ggagagcatg accgaccgca
     2401 tccagaagga agcggaggcc tacatcgcca cgatcgacgg cctcggcggc gcggtcaagg
     2461 cgatagagaa gggctaccca cagcaggaga tccaggacgc cgcctaccag taccagatgg
     2521 acgtcgagtc cggcgagcgc gtcgtggtc
//
LOCUS       DAOOLN010000149         2546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_50307_length_2546_cov_2.502208, whole genome shotgun sequence.
ACCESSION   DAOOLN010000149 DAOOLN010000000
VERSION     DAOOLN010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50307_length_2546_cov_2.502208"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="PLW80_02680"
     CDS             <1..912
                     /locus_tag="PLW80_02680"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB65437.1"
                     /translation="RIQSALLAGALGLVFALPYIAVVLGTKERADFSAAPPRKDWWTP
                     MLNAFKVRSFRMLIVVYLAVFVSLDLITTSFQFYMTYVLRRPDEFASVLGVLIVVEIV
                     AALFTAPLVRLTNKATATILGSALWMLTGLATLLVGPDSPGFAVYLVAAVMGVAMAFP
                     VVLLNSLFADVSDVGELYFGSRVEGTFSGVQTFVRKVASAGANASFMLAIGWAGFVAP
                     VRRVEGLRETLTFQAQPTAFETAIRATIAIAPLALLGLGIAVMLRWPITSERHGKLLE
                     YLAAAREGRECEPGLKAEVEALRDAVL"
     gene            913..2388
                     /locus_tag="PLW80_02685"
     CDS             913..2388
                     /locus_tag="PLW80_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HPB65438.1"
                     /translation="MTRPVLRLKPPTGWMNDPNGFIHSGGRYHLFYQHNPYAPRWGRM
                     HWGHAVSDDLIRWEHLPVALGPDSFWDGALGCFSGSAIERGGELCLLYTGVSILGQYQ
                     MLARSGDGVAFAKRRRPVIGRRLLPPGGSGRAFRDPKLFERDGAFYCVVGGDWKPEAP
                     AGGGGSRTARGTQVSLYRSDDLVEWSYVGPLLRGVLPDGGVFECPDYIRVDGKDVLIA
                     SPMHLPRNDEGEFENLHSTVYLPGALDLERGLFEPDDGPGVYRELDGGTDFYAAQTTT
                     APDGRALMVAWMQMWKRTMPTEAEGWAGAMTVPRELSYEGGRLRQRPVRELERYWADE
                     VRGPVGSIDGVEPLPAVGDGPFDIELAVDLGSSSRFGVRLFVPTGSAPPEPDGPCLIV
                     AYDRSSETLSIDRSRAPVAISSSDRREAQCLTRATRLRLDGRLLRLRLIVDGPYVELF
                     GDDGACALSTLYYAPGDHRGAAVISDGPARVVELVRRRIAI"
BASE COUNT          355 a          911 c          867 g          413 t
ORIGIN      
        1 cgcatccaga gcgcattgct cgccggagca ctgggcctcg tcttcgcgct gccgtatatc
       61 gccgtcgtgc tcggtacgaa ggaacgcgcc gacttctcgg cagccccgcc gcgcaaggac
      121 tggtggacgc cgatgctgaa cgccttcaag gtgcgtagct tccgcatgct catagtcgtc
      181 tacctcgccg tgttcgtgtc gctcgacctc atcaccacct cgttccagtt ctacatgacc
      241 tacgtgctgc gccgaccgga cgagttcgcc tcggtcctcg gcgtgctcat cgtcgtcgag
      301 atagtcgccg cgctgttcac ggctcccctc gtcaggctta cgaacaaggc cacggcgacg
      361 atactgggct ccgcgctatg gatgctcacg gggctcgcga ccctgctggt cggccccgac
      421 agccccggct tcgcggtgta cctcgtcgcg gcggtcatgg gcgtcgccat ggcgttcccg
      481 gtcgtgctgc tcaatagcct gttcgccgac gtcagcgacg tgggcgagct gtatttcgga
      541 agccgggtcg agggcacctt ctcgggcgtg cagaccttcg tgcgcaaggt agcctccgcc
      601 ggcgccaacg cgtccttcat gctcgcgata ggctgggccg gcttcgtggc gcccgtcagg
      661 cgcgtcgagg ggctcaggga gacgctgacg ttccaggccc agccgacggc gttcgagacg
      721 gcgatacgcg ccaccatagc catcgcgccg ttagcgctac tcggcctggg catagcggtc
      781 atgctgcgct ggcctatcac gagcgagcgg cacggcaagc tgctggagta cctggccgcg
      841 gcgcgcgagg gccgcgagtg cgagcccggc ctcaaggccg aggtcgaggc cttgcgcgac
      901 gcggtgctct agatgacgcg gccggtactg cgcctgaagc cccctacggg ctggatgaac
      961 gacccgaacg gctttatcca ctcgggcggg cgataccatc tcttctatca gcacaacccc
     1021 tacgcgccgc gctggggccg catgcattgg ggccacgccg tctcggacga cctgatacgc
     1081 tgggagcacc tgcccgtcgc gctcgggccg gactcgttct gggacggcgc gctcggctgc
     1141 ttctcgggca gcgcgatcga gcgcggcggc gagctatgcc tcctctacac cggcgtgtcg
     1201 attctcggcc agtaccagat gctagcgcgc tccggcgacg gcgtagcgtt cgccaagcgg
     1261 cggcgtccgg tcataggccg ccgcctgctg cctccgggcg gctcgggccg cgccttccgc
     1321 gaccctaagc tgttcgagcg cgacggcgcc ttctactgcg tcgtgggcgg cgactggaag
     1381 cccgaggcgc cggcgggcgg gggagggagc cggaccgcgc gcggtacgca ggtatcgctg
     1441 taccgctccg acgacctggt cgagtggagc tacgtcggcc cgctgctgcg cggcgtcctc
     1501 ccggacggcg gcgtcttcga gtgccccgac tatatacgcg tcgacggcaa ggacgtcctg
     1561 atcgctagcc cgatgcacct gccgcgtaac gacgaagggg aattcgagaa cctgcactcg
     1621 accgtctacc tgcccggcgc cctggacctc gagcgcgggc tcttcgagcc cgacgacggc
     1681 ccgggcgtct accgcgagct cgacggcggc acggatttct acgccgccca gacgacgacg
     1741 gcgcccgacg gccgcgccct gatggtcgcc tggatgcaga tgtggaagcg gaccatgccg
     1801 accgaggccg agggctgggc cggcgcgatg accgtgccgc gcgagctgtc gtacgagggc
     1861 ggccggctca ggcagcgccc ggtacgggag ctcgagcggt actgggccga cgaggtccgc
     1921 gggccggtcg gctcgataga cggcgtcgag ccgctgcccg cggtcggcga cggccccttc
     1981 gatatcgagc tcgccgtcga cctcggctcg tcgtcgaggt tcggcgttcg tctcttcgtc
     2041 cctaccggat cagcgccccc ggaaccggac gggccatgcc tgatcgtcgc ctacgacagg
     2101 tcgagcgaga ccctcagcat agaccgatcg cgcgcccccg tcgccatatc gtcgtctgac
     2161 cgcagggagg cgcaatgcct gacgcgcgct acgaggctca ggctcgacgg gaggctcctg
     2221 aggctcaggc tgatcgtcga cggcccgtac gtcgagctat tcggcgacga cggggcctgc
     2281 gccttgtcga cgctctatta cgccccgggc gatcaccgcg gcgcggcggt catctccgac
     2341 gggccggcgc gcgtcgtcga gctggtcagg cgccgcatag ccatctaggc gctcagcctt
     2401 gcccatgccc gggcggccgg gctatactcc cgatcgcgcg ctagccgatc ggtccgccga
     2461 gccgcgagcg cgacaccaaa acccagcctc gaggccctcc gccgggcgcg ccctctggcg
     2521 cgaattcccc aaccgttgcc tgcacg
//
LOCUS       DAOOLN010000150         2543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_50390_length_2543_cov_3.202974, whole genome shotgun sequence.
ACCESSION   DAOOLN010000150 DAOOLN010000000
VERSION     DAOOLN010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50390_length_2543_cov_3.202974"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PLW80_02690"
     CDS             complement(<1..916)
                     /locus_tag="PLW80_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPB65439.1"
                     /translation="MAHTSTYRINGEDLVFETGRMAKQANGSVFARFGGSAVIATACC
                     SDKVTEGLDFVPLTVEYNEKYYAAGKIPGGFIKREARPKDREILVSRLIDRPMRPLFD
                     KAFGREIQVVPTTVSADLVNPPDIIAINAASAAVHISDIPFNGPIAAVRVCYVGGTYV
                     INPTYDQIEKSALEIVVAGSEEGITMVEGGADEVSEELMIGAIEAAKKPIADICGVIR
                     AMRDAVGKPKLALAPLKAELARKAEIEAFGKERIGKAIFTKVKHERYAAVAKAIAETK
                     ATFADSLGDDEVQLKLFAALMEDIQYNLL"
     gene            complement(995..1264)
                     /gene="rpsO"
                     /locus_tag="PLW80_02695"
     CDS             complement(995..1264)
                     /gene="rpsO"
                     /locus_tag="PLW80_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPB65440.1"
                     /translation="MAVTKEDKARLVKEYGTSEKDTGATKVQIALLTERISGLTEHFK
                     VNKKDTNSKRGLMILIGQRRRLLKYLQRKDLNTYRDICDKLGIRK"
     gene            complement(1290..2090)
                     /locus_tag="PLW80_02700"
     CDS             complement(1290..2090)
                     /locus_tag="PLW80_02700"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase family protein"
                     /protein_id="HPB65441.1"
                     /translation="MRVVDWAEAVSGRAPMPEHSAATIGVFDGLHLGHRALIERVLAK
                     GPGLAPVIVTFRDNPKRITRPHRFSGSFFTLEQKMAALEEAGAEVCVLIDFSGNFSKL
                     AGSEFVSALGSIGVRSFVIGSDFKCGHRLSTDAEGLKAVAASFGADTEIVGPVTLGGE
                     PVSSSRIRAAIANGRTDLALAMLGRPYTLDLRRLEIGYERGSAAFSFKGSGLVEPAPG
                     RYEATEICVAGSGAVEATLRRDGTVHWARTALCADHEPAFLAFGSRIA"
     gene            complement(2087..>2543)
                     /locus_tag="PLW80_02705"
     CDS             complement(2087..>2543)
                     /locus_tag="PLW80_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65442.1"
                     /translation="RPVTIRSLELLSYEGGVARLRVSCTKGTYIRSLARDLALACGSR
                     GRLSALRRTFSGPFTAEAAIRADAFGPDSLRFLSEPDARGLNLGVVRLAGEEAHAFSN
                     GLPLFRLSTFAGLAGQGPVVAFDEAGRLLGVVSRASGAWRYSFVLGGEV"
BASE COUNT          452 a          805 c          810 g          476 t
ORIGIN      
        1 gcagtaggtt atactggatg tcctccatca gagcggcgaa cagcttgagc tggacttcgt
       61 cgtcgccgag cgagtcggcg aaagttgcct tggtctcggc gatggccttg gcgaccgcgg
      121 cgtaacgctc atgcttgacc ttggtgaaaa tagccttgcc gatgcgctcc ttgccgaagg
      181 cctcgatctc ggccttgcgg gcgagctcgg ccttaagcgg cgcgagcgcc agcttgggct
      241 taccgacggc gtcgcgcatg gcgcggatga cgccgcagat gtcggcgata ggtttcttgg
      301 cggcctcgat agcgccgatc atcagttcct cggacacctc gtcggcgccg ccctcaacca
      361 tcgtgatgcc ttcctcggaa ccggcgacga cgatctccag ggccgacttc tcgatctggt
      421 cgtaggtcgg attgatgacg taggtgccgc cgacgtagca gacgcgcacg gcggctatgg
      481 gtccgttgaa cgggatgtcg ctgatatgaa cggcggcgct ggccgcgttg atggcaataa
      541 tgtcgggggg gttgacgagg tcggcggaga ccgtcgtcgg cacaacctgg atctcgcgtc
      601 cgaaggcctt gtcgaacagc gggcgcatcg gcctatcgat gagcctggag acgaggatct
      661 ccctgtcctt gggcctggcc tcgcgcttga tgaacccgcc ggggatcttt ccggcggcgt
      721 agtacttctc gttgtactcg acggtaagcg gtacgaagtc gaggccctcg gttaccttgt
      781 cggagcagca ggcggtggct atgaccgcgg agccgccgaa acgggcgaag accgagccgt
      841 tggcctgttt ggccatgcgc ccggtctcga acacgaggtc ttcgccgtta attctatacg
      901 tgctggtatg tgccatatgc gatgggtctc catgggccgc gttcggccta atgacgaaag
      961 agcccgaacc gtcgttcggg ctccgataag acgattattt acggatgcct agcttgtcgc
     1021 agatgtcgcg atacgtgttc aggtccttgc gctgcaggta cttgagcaag cgcctccgct
     1081 ggccgatgag gatcatcagc ccgcgcttgg agttggtgtc cttcttgtta accttgaagt
     1141 gctcggtgag accgctgatg cgctcggtga gcaacgcgat ctggaccttg gtcgcgccgg
     1201 tgtccttctc gctggtacca tactccttga cgagcctggc cttatcctct ttcgtcactg
     1261 ccatgtctat aaactcctta ttgccgttat catgcgatcc tcgagccgaa cgcgaggaag
     1321 gcgggctcgt gatcggcgca taacgccgtc ctcgcccagt gtaccgtgcc gtcccgtcgc
     1381 agagtcgcct ccacggcgcc gctaccggcg acgcatatct ccgtcgcctc gtacctgccg
     1441 ggcgcgggct ccaccaaacc gctacccttg aacgagaacg ccgcggagcc tcgttcatat
     1501 ccgatttcga gccgccttag atccaaggtg taggggcgac cgagcatggc gagtgccagg
     1561 tccgtccttc cgttcgcgat agccgcgcgt atccggctcg agctgactgg ctccccgcca
     1621 agcgtgacag ggccgacgat ctccgtatcc gcgccgaagg aggccgctac ggccttgaga
     1681 ccctcggcgt cggtggatag tcggtgcccg cacttgaagt cgctaccgat gacgaacgaa
     1741 cgaacgccga tggaccctag ggccgaaacg aattcgctcc cggccagttt actgaaattc
     1801 ccggaaaagt caatcaggac gcagacctcc gcacccgctt cctcgagggc ggccatcttc
     1861 tgctcgaggg taaagaagct tccagagaag cgatgcggcc tcgttatccg tttcggattg
     1921 tccctgaagg tgactatgac cggcgccagg ccgggcccct tggcaaggac acgctcgatg
     1981 agcgcccgat gcccgaggtg caagccatcg aagacgccta tcgtggcggc cgagtgctcg
     2041 ggcatgggcg cacggcccga gaccgcttcg gcccaatcta cgaccctcac acctcgcccc
     2101 cgagcacgaa ggagtagcgc catgcgccgc tcgcgcgaga gacgacgcca aggagccggc
     2161 cagcctcgtc gaacgcgacg accggtccct gccctgccag gcccgcgaag gtagacagcc
     2221 tgaacaaggg cagtccgttc gagaacgcgt gggcctcttc gcccgccagg cggacgacgc
     2281 cgaggttaag gcctcgagcg tcaggttcgg acaggaagcg cagactatcg ggcccgaagg
     2341 catcggcgcg tatggccgcc tccgcggtga acggccccga aaaggtgcgc cggagcgccg
     2401 aaagcctacc acgcgagccg caggccagcg ccaggtccct ggctaacgag cgaatatagg
     2461 tacccttggt gcacgagacg cgcagcctgg ccacgccgcc ttcgtaggat aacagctcga
     2521 gggagcgtat cgtcacgggt cgc
//
LOCUS       DAOOLN010000151         2535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_50592_length_2535_cov_3.642339, whole genome shotgun sequence.
ACCESSION   DAOOLN010000151 DAOOLN010000000
VERSION     DAOOLN010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50592_length_2535_cov_3.642339"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1975)
                     /locus_tag="PLW80_02710"
     CDS             complement(<1..1975)
                     /locus_tag="PLW80_02710"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPB65443.1"
                     /translation="MSRYDKYSDSALDVLVKTQDIALRGPIGETGHRDLAEALMLFDL
                     PDIIAIAKSNNAVVDVELIKSLNERLESSAPREVEGPIKISGEFRSILGRAEALAGDA
                     KVEPSHLIRAAWPTIKGELAPFFRLEAAPALAIPADEIALPEIGPRAESAEAIAALLR
                     FGDELTGPDADFPVFGREAELDALSSVLMRYWKPNPLIVGESGVGKTALVQGLAMRIK
                     SGDVPARLRNARVFEVRVSELLAGTAMHGALEENVNQLVEAAESLQDVYLFIDEIHQV
                     VPAFANNPISETLKPALSSGRIRCIGATTSADYARYLEKDTALLRRFQTVLVKEPDET
                     TIGRIVAGVAPRLERHFGVSIPEPVRARSVEVARRYLPMRRFPDKALDVLDRASARAA
                     FRGDEELAERHLLEAVKDIANVVVEPGLDEASGLAGLEAAIGRDVLRQEAAIKSVSDA
                     VRVARMRLDPRRDRPSGAFLLTGPTGVGKTAFAERLAKALSGRDDALFRIDMSEFSDG
                     HTASRLLGAPPGYIGYDDAPLLSKAVDACSGGVLLLDEFEKAHQQVHRVFLQILDAGR
                     ATDSTGRTLSFSGLTIVATCNVGGDAGPALGFLAGTEAAALAASGTVPMAALRRAFPI
                     ELLNRFDAIVPFRALDRDDARAILTGVVVRDANE"
     gene            complement(1978..>2535)
                     /locus_tag="PLW80_02715"
     CDS             complement(1978..>2535)
                     /locus_tag="PLW80_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65444.1"
                     /translation="TKLSTIESYLIAERLKYSVHDDYIRTSFATDLYRDQDGDASVFI
                     IARVEEDGEYFKLMAPNLYRYPPDGPNAGWVYRTLLGICWRSKLIKYEYDERDGEIRA
                     IVEFPIEDAELGPRQFMRCLNGLVQIIDEYHPAVAAAMAGGPASLDDAEETADNVRRA
                     ERILRAAGVDRAKLSLAGSPVRLEE"
BASE COUNT          368 a          846 c          898 g          423 t
ORIGIN      
        1 tctcgttggc gtcgcggacc acgacgccgg tcagaatggc gcgagcgtcg tccctgtcga
       61 gggcccggaa cggcacgatg gcgtcgaagc ggttgagcag ctcgatcggg aaggccctac
      121 gcagggccgc catcggcacc gtgcccgaag ccgcgagcgc ggccgcctcc gtgccggcca
      181 ggaagccgag cgcggggccg gcgtctcccc cgacgttgca ggtggccacg atggtcaggc
      241 ccgagaacga cagcgttctg ccggtagaat cggtcgcgcg gcccgcgtcg agtatctgca
      301 ggaagacgcg gtgcacttgc tgatgggcct tctcgaactc gtcgaggagg agcacccccc
      361 cggagcacgc gtcgaccgcc ttggacagga gcggggcgtc gtcgtagccg atgtagccgg
      421 gaggcgcgcc gaggaggcgc gaggcggtat ggccgtctga gaactcgctc atgtcgatgc
      481 ggaacagcgc gtcgtcgcgg ccggataggg ccttggccag tcgctcggcg aaggccgtct
      541 tgccgacgcc cgtcgggccg gtcagcagga aggcgccgct cggcctgtcc ctgcgcgggt
      601 cgagccgcat cctggccacc ctgacggcgt cggagacgga cttgatcgcc gcttcctgcc
      661 tgagcacgtc gcggccgatg gcggcctcga ggccggccag cccgctcgcc tcgtcgaggc
      721 ccggctcgac caccacgttg gctatgtcct tgaccgcctc gaggaggtgt cgctccgcga
      781 gctcttcgtc gccgcggaac gcggccctgg cggaggcccg gtcgagcacg tcgagcgcct
      841 tgtcggggaa gcggcgcatc ggcaggtagc gcctggccac ctcgaccgag cgagcgcgga
      901 caggctccgg tatcgatacg ccgaaatggc gctcgagcct cggggcgacg ccagcgacga
      961 tacggcctat cgtcgtctcg tcgggctcct tgaccagcac ggtctggaag cgcctgagca
     1021 gggcggtgtc cttttcaagg tagcgcgcgt agtcggcgct cgtcgtcgcc ccgatacagc
     1081 gtatgcgccc ggaggacagg gccggcttga gggtctccga gatcgggttg ttggcgaagg
     1141 cgggcacgac ctggtggatc tcgtctatga acaggtagac gtcctggagg ctctcggccg
     1201 cctcgacgag ctggttgacg ttctcctcca gggcgccgtg catcgcggtg ccggcgagga
     1261 gctcggagac gcgcacctcg aagacgcggg cgttcctgag gcgggccggc acgtcgcccg
     1321 acttgatccg catcgcgagg ccctggacga gggcggtctt gccgacgccc gactcgccga
     1381 cgatcagcgg gttgggcttc cagtatcgca tgagcaccga gctgagcgcg tcgagctcgg
     1441 cctcgcggcc gaagaccggg aagtcggcgt ccggcccggt cagttcgtcg ccgaagcgca
     1501 ggagcgcggc tatcgcctcg gcggattcag cccgcggtcc tatctcgggc aacgctatct
     1561 cgtcggccgg gatggccagg gcgggcgccg cctccagcct gaagaacggc gccagttcgc
     1621 ccttgatcgt cggccaggcg gcccgtatga ggtgggacgg ctcgaccttc gcgtcgcccg
     1681 cgagcgcctc ggcccgcccg agtatcgacc tgaactcgcc ggagatcttg atcggcccct
     1741 cgacctcgcg cggggcgctc gactcgaggc gctcgttgag cgacttgatc agctccacgt
     1801 cgacgaccgc gttgttgctc ttggcgatcg cgatgatgtc cggcaggtcg aacagcatca
     1861 gggcctcggc caggtcgcgg tggccggtct ccccgatggg gccgcgcagg gctatgtcct
     1921 gagtcttgac gaggacgtcc agggcgctgt ccgaatattt atcgtacctg gacatgccta
     1981 ctcctcgagc ctgaccgggg atccggctag gctcaacttg gcccggtcga cgccggcggc
     2041 cctcaggatc cgctcggccc tgcgcacgtt gtcggccgtt tcctccgcgt cgtcgagcga
     2101 cgccgggccg ccggccatgg cagcggcgac ggccgggtgg tactcgtcga tgatctgcac
     2161 gagaccgttc aggcaacgca tgaactgcct ggggccgagc tcggcgtcct cgatcgggaa
     2221 ttcgacgatg gcccgtatct cgccgtcccg ctcgtcgtac tcgtacttga tcagcttgga
     2281 acgccagcag ataccgagca gggtccggta cacccagccg gcgttcggcc cgtcgggcgg
     2341 atagcggtag aggttcggcg ccatcagctt gaagtactcg ccgtcctcct cgaccctggc
     2401 gatgatgaac acgctcgcgt cgccgtcctg gtcgcggtat aggtcggtgg cgaagctcgt
     2461 acgtatataa tcgtcgtgca ccgagtattt gagccgctcg gcgatcaggt acgattctat
     2521 ggtcgacagc ttcgt
//
LOCUS       DAOOLN010000152         2529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_50753_length_2529_cov_3.399757, whole genome shotgun sequence.
ACCESSION   DAOOLN010000152 DAOOLN010000000
VERSION     DAOOLN010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50753_length_2529_cov_3.399757"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PLW80_02720"
     CDS             complement(<1..472)
                     /locus_tag="PLW80_02720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB65445.1"
                     /translation="MTAPDDIFRASFVQARQAMIVLDERGIPALWNAAFEELFTELAG
                     FVPERLAVPLFDWLEERESFGFSYYVTEVLHGRMGKAVVETGARTATGRRLWLRTTLS
                     TLRLDGDGRWIWCSFQDVTEQKLKERELVSAKEEAEKATQTKSQFLANMSHEIRT"
     gene            complement(503..1786)
                     /locus_tag="PLW80_02725"
     CDS             complement(503..1786)
                     /locus_tag="PLW80_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB65446.1"
                     /translation="MAKASVLIIDESDLFRDYLKARLSRAGLVATAAINGLDGISKMR
                     DDPPDLVVMDYHLTRKTCQEVLEEKARNPNTASVPVVLTAHKIDKNRLLELVRYNIRK
                     VFMKPIKMDGFYQIVSEITGARFEVDKTPCIVEAHVNDNIVFVELAKGINLEKVDLLR
                     FKIRELLDLYAIQKPRVLVMLSDMELSFVDGPNLEALLETIAEASRAKPRHIKVLTRS
                     SFAKEYVSGQPRFAEIEVVSSLEYALDGLVAEAARAADAPDDKAAIITDRILSAQGSA
                     SGGESVEMRFEAERVEAPRLSIESARHLGDGLSIAVVDDDFVIQELLKTTFEAIDAKV
                     HTFPNGRAFLTAVRSRVFDLVFLDLRMPEVDGFGVLAELSAQDLEMPIIVLSAVTQRE
                     SVVRAFKAGVKSYLVKPLKPEQILRKTLEILKANF"
     gene            complement(1788..>2529)
                     /locus_tag="PLW80_02730"
     CDS             complement(1788..>2529)
                     /locus_tag="PLW80_02730"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPB65447.1"
                     /translation="VAVVGIPKTIDNDLLFVDRSFGFETAVERAADAVTAAHAEAHSS
                     INGVGLVKLMGRDSGFIAVHTALATHEANFVLIPEAPFDLGGPNGLLAHLERRLQARN
                     HAVIVVAEGAGQDAIALDPSATDASGNRRLGDVGAWLKDAITAHFKARGLELNLKYID
                     PSYMIRSAPACPTDSVYCERLGNNAVHAAMAGKTRVLIGMVNNEFVHLPTGVVVTRRN
                     TVDPEGSLYRDAIDATGQPVSMRNAREA"
BASE COUNT          382 a          816 c          881 g          450 t
ORIGIN      
        1 gcgtgcgtat ctcgtggctc atgttggcca ggaactggct cttggtctgg gtggctttct
       61 cggcctcttc cttggccgag acgagctcgc gctccttgag cttctgctcg gtgacgtcct
      121 ggaacgagca ccagatccag cgtccgtcgc cgtcgagccg gagcgtcgac agagtggtcc
      181 gtagccagag gcggcggccg gtggccgtgc gcgcgccggt ctcgacgacg gccttgccca
      241 tgcggccgtg cagcacctcg gtcacgtaat acgagaagcc gaaggattcg cgctcctcga
      301 gccagtcgaa cagcggcacc gccagccgtt cgggcacgaa accggccagc tcggtgaaca
      361 gctcctcgaa cgcggcgttc cagagggcgg gtatgccccg ctcgtcgagc acgatcatgg
      421 cctgcctggc ctggacgaac gaggcgcgga agatgtcgtc gggcgctgtc atgatcgctc
      481 ctgcgccgcc acgggcgact cgctagaaat tggccttgag tatctccagg gtcttcctga
      541 ggatctgctc gggcttgagc ggcttgacca ggtagctctt gacgccggcc ttgaaggccc
      601 tgaccaccga ctcgcgctgg gtcaccgcgg acagcacgat gatcggcatc tcgaggtcct
      661 gggcggacag ctcggcgagt accccgaacc cgtccacctc cggcatgcgc aggtcgagga
      721 agaccaggtc gaacaccctc gagcgcaccg cggtcaggaa cgccctgccg ttcgggaagg
      781 tgtgcacctt ggcgtcgatc gcctcgaagg tggtcttgag cagttcctgg atgacgaagt
      841 cgtcgtcgac cacggctatc gacaggccgt cgccgaggtg cctggccgac tcgatcgaca
      901 gccgcggcgc ctcgacgcgc tccgcctcga agcgcatctc caccgactct ccgccggacg
      961 ccgagccctg ggccgacagg atgcggtcgg tgatgatagc agccttgtcg tcgggggcgt
     1021 ccgcggccct cgccgcctcc gcgaccagcc cgtcgagggc gtactcgagg ctcgagacca
     1081 cctcgatctc ggcgaagcgc ggctggccgg agacgtactc cttggcgaag ctcgagcggg
     1141 tcagcacctt gatgtgcctg ggcttggcgc gcgacgcctc ggcgatcgtc tcgagcaggg
     1201 cctcgaggtt cggcccgtcg acgaagctca gctccatgtc ggagagcatg acgagcaccc
     1261 ggggcttctg gatggcgtag aggtcgagca gctcgcggat cttgaagcgc aggaggtcga
     1321 ccttctcgag gttgatcccc ttggccagct cgacgaacac gatgttgtcg ttcacgtggg
     1381 cctcgacgat gcagggcgtc ttgtcgacct cgaagcgggc gccggtgatc tcggagacga
     1441 tctggtagaa gccgtccatc ttgatcggct tcatgaacac cttgcgtatg ttgtagcgga
     1501 ccagctcgag caggcggttc ttgtctatct tgtgggcggt gagcacgacg gggacggacg
     1561 cggtgttcgg gttgcgcgcc ttctcctcga gcacctcctg gcaggtcttg cgcgtcaggt
     1621 ggtagtccat gacgacgagg tcgggcgggt cgtcgcgcat cttcgagatg ccgtcgaggc
     1681 cgttgatcgc cgcggtggcc acgaggccgg cccggctcag cctggccttg agatagtcgc
     1741 ggaacaggtc cgactcgtcg atgatgagca cgctggcctt ggccacgcta cgcctcccgc
     1801 gcgttgcgca tcgagacggg ctggccggtc gcgtcgatgg cgtcgcggta caggctgccc
     1861 tccggatcca ccgtgttgcg ccgggtcacg acgacgccgg tgggcagatg gacgaactca
     1921 ttgttgacca tgccgatcag gacgcgggtc ttgccggcca tagcggcgtg gaccgcgttg
     1981 ttgcccaggc gctcgcagta gaccgagtcg gtcgggcagg ccggagcgct ccgtatcatg
     2041 tagctcgggt cgatgtactt gaggttcagt tcgaggccgc gcgccttgaa gtgggccgtg
     2101 atggcatcct tgagccaagc gccgacgtcg ccgaggcgcc ggttgcccga ggcgtcggtc
     2161 gccgacgggt ccaaggcgat cgcgtcctgg ccggctccct ccgccacgac gatgacggcg
     2221 tggttcctgg cctggagcct gcgctccagg tgcgcgagca gcccgttcgg gccgcccagg
     2281 tcgaacggcg cctcggggat cagcacgaag ttggcctcgt gggtggcgag cgccgtatgc
     2341 accgcgatga agcccgagtc gcggcccatc agcttgacga gcccgacgcc gttgatcgac
     2401 gagtgggcct cggcgtgggc ggccgtcacc gcgtcggcgg cccgctcgac ggccgtctcg
     2461 aaaccgaagc tacggtccac gaacagaagg tcgttgtcga tggtcttggg tatgccgacg
     2521 acggctacc
//
LOCUS       DAOOLN010000153         2526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_50854_length_2526_cov_2.152975, whole genome shotgun sequence.
ACCESSION   DAOOLN010000153 DAOOLN010000000
VERSION     DAOOLN010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50854_length_2526_cov_2.152975"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..627)
                     /locus_tag="PLW80_02735"
     CDS             complement(178..627)
                     /locus_tag="PLW80_02735"
                     /inference="COORDINATES: protein motif:HMM:NF024784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HPB65448.1"
                     /translation="MRVISHTIHYGIGELRSMIVKKPRGGEYEPLPGPTGRALWEDDS
                     AELPGERFALDPDTGAEGSNYGRVRHGGSILRQSPDEGKASDWLHVSIPGHGDVNVWR
                     VIAGAWCERRDGATQVHHINNNGFDNRPSNLLWVSQAEHRAIEQGAF"
     gene            839..1609
                     /locus_tag="PLW80_02740"
     CDS             839..1609
                     /locus_tag="PLW80_02740"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HPB65449.1"
                     /translation="MQAWLRTDPLYKDALTDVWLWNDFPGRKDFGGKDTGIDLVARTG
                     SGDYWAIQCKCYAEDAIIDKPAVDSFLATSSRSFLDDQLRTTTFSNRLWISTTNHWGP
                     NAEETIHNQKPPVHSVALSDVLASPVDWDALERGVHGGKKKEIDARKPKGFPDHIPFI
                     HAIPDPHVERWVLNDSAAFKAVFGKGCAAPDQKCEKDRYKDILNRAIVAAGGEITISW
                     TEHAEELIEHMDIDHLCRVDESVEKFIGECRAAFKALA"
     gene            1892..>2526
                     /locus_tag="PLW80_02745"
     CDS             1892..>2526
                     /locus_tag="PLW80_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65450.1"
                     /translation="MARIKGAETIYEAARAWGEACLVGDGSLFGSERLWTAERLAELK
                     RLYIDRPDESDRSFMDKLEDQLADGSPESKRLAAELFWPILLFTSAQKPATKIVKMRR
                     VWQWSGSSFPEDLPMLVPSLLAGIGSAGPALNNLFWLEYAFAIRLFLAIKAKPASERR
                     ELLADGWAFAAFMDGLDEAHGRQCYHMLAHLLFPDFFERIASRSHKAAIVNT"
BASE COUNT          515 a          752 c          769 g          490 t
ORIGIN      
        1 ccgccgcggg cttcctcggc gatcgcgggc ttgctcggcg atcgcggact tcctcggcga
       61 tcgcggacta aagccgcaat cgctgccgtc gttgaacctg actcgccatg ctcgcaggtt
      121 aacgacggcg cccgggtacg agcctcggcg atcccgcggc tccccgggga aacgcgccta
      181 gaacgcgccc tgctcgatgg cgcggtgctc ggcctgcgat acccagagca ggttggaagg
      241 ccggttgtcg aagccgttgt tgttgatgtg atgcacctgg gtcgcgccgt cgcgccgctc
      301 gcaccacgcg ccggctatca cgcgccagac attgacgtcc ccgtgcccgg ggatggagac
      361 gtggagccaa tcgctcgcct tgccctcgtc cggggattgc ctgagtatcg agccgccatg
      421 gcgcacgcgc ccgtagttgc tgccctcggc cccggtatcc ggatcgagcg cgaagcgctc
      481 gcccggcaat tcggcgctgt cgtcttccca cagcgcccgt cccgtgggcc cgggcagggg
      541 ctcgtactcg cctcccctcg gcttcttgac tatcatcgag cgtagctcgc ctatgccgta
      601 gtggatcgta tggctgatga cgcgcatggt tcctcctgcg tagagcatag catgggggag
      661 gagccgtggg gagcaaggca ggcacggtcg acacgttctg ttgccctcga ccgatcgagg
      721 ctatacaatg ctcgtattcg taacgacggg gtaaaacatg ggtttcgagg caatcctcaa
      781 tcactataga tcgcattcat tctccgaacg cgataagggc gcccgtttcg agcgcctcat
      841 gcaggcctgg ttgcgtacgg atccgctcta taaagatgcc ttgacggacg tctggctctg
      901 gaacgatttc cccgggcgta aagatttcgg cggtaaggat accggcatag accttgtggc
      961 tcgtaccggg agcggcgact attgggccat ccagtgtaag tgctacgccg aggacgcgat
     1021 catcgacaag cccgccgtcg atagcttcct ggccacctcg agccgctcct tcctggacga
     1081 ccagctacgc acgacgacct tctccaaccg gctatggata tccacgacca atcattgggg
     1141 cccgaacgcc gaagaaacaa tacataacca gaagcccccg gtacacagtg tagcactatc
     1201 cgacgtgctg gcttccccgg tggactggga cgcgctcgag cgtggcgttc atggcggcaa
     1261 gaaaaaggaa atcgacgcaa ggaaacctaa gggtttcccc gatcacatcc cgttcataca
     1321 cgctataccc gatccccacg tcgagcgatg ggtgctgaat gactcagcgg cgttcaaagc
     1381 ggttttcgga aaaggatgcg ccgcgcccga ccagaaatgc gagaaggata ggtataaaga
     1441 catactgaac agagcgatag tcgccgccgg aggagagata accatcagct ggaccgagca
     1501 cgcggaagaa ctcatagaac atatggatat cgatcatctg tgccgcgtcg acgagtcagt
     1561 cgaaaagttc attggagaat gccgcgccgc tttcaaagcg cttgcctgaa tccttaaaaa
     1621 agggaaccgg cgattgaccg gtccctcggc tccttccgcc atgggtggat gagtatcaca
     1681 aggcgaaaca tagctcttgt attgttttct tacaagagag ttaagtaccg cggagacact
     1741 tcatggcgat gagcaaaggc ggtggcgttg gcgttggcgg ggtgatgcta acggcaacat
     1801 gtaaggaagg cttacacgtt gcgtgtatac agaccagaat gaaccgtccg gtaaatccag
     1861 atggttcgca tataaagcac ggaggcgtct gatggctagg atcaaaggag ctgaaacgat
     1921 atacgaggct gcgcgggcgt ggggcgaggc gtgcctcgtc ggggacgggt cgctgttcgg
     1981 cagcgagcgg ctctggacgg ccgagcgcct ggcagagctt aagcggctat atatagaccg
     2041 tccggacgaa tcggatcgtt cgttcatgga caagctcgag gaccagctgg cagacggctc
     2101 gcccgagtcc aagcgactcg ccgccgagct cttctggccc atattgctct ttacatcggc
     2161 acagaagccg gcgaccaaaa tcgtgaagat gcgccgtgtc tggcagtggt caggctcgtc
     2221 cttccccgag gacctcccta tgctcgtccc gtcgctgctc gcgggcatag gcagcgccgg
     2281 accagccttg aacaatctct tctggctcga gtatgccttc gccatcaggc tgttcctagc
     2341 tatcaaggcc aagcccgcgt cggagcggcg ggaactgctg gcggatggct gggcctttgc
     2401 ggcattcatg gacgggctgg acgaggccca tggtcgtcaa tgctaccaca tgctcgcgca
     2461 tctcctcttt cccgatttct tcgaacggat cgcgagccgt agccacaagg cggctatcgt
     2521 caatac
//
LOCUS       DAOOLN010000154         2505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_51451_length_2505_cov_3.229796, whole genome shotgun sequence.
ACCESSION   DAOOLN010000154 DAOOLN010000000
VERSION     DAOOLN010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51451_length_2505_cov_3.229796"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PLW80_02750"
     CDS             complement(<1..608)
                     /locus_tag="PLW80_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65451.1"
                     /translation="MAKTVFRPQETVPLSSAYVLHAPGGDIVVEDVEAVAPALEYEGP
                     TADDLRAEAELFSQNWEREKEAMMAAARAEAQDIVAKAEAAAFEEVRRKNDQALKIRR
                     EAESAAEKAIAEAEAKIQAFEAQAKARVDEVTKGACKDGLDQGREEGFKEGKLEVERV
                     VGRREERGFARLEQHALQVPGESRAPERVAEHGTGKGRHGLGA"
     gene            complement(622..1701)
                     /gene="fliG"
                     /locus_tag="PLW80_02755"
     CDS             complement(622..1701)
                     /gene="fliG"
                     /locus_tag="PLW80_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HPB65452.1"
                     /translation="MPQAAPAKGKSTGPGASGSTGKKPKSAKDLNGRQKAAIFLITLG
                     SEISAEIFKHLREDEIETLTFEIARLENVEPELKDAILMEFQELMMASEFISSGGIDY
                     ARELLEKALGSQKAIDVINRLTSSLQVRPFDFIRRTDPANLLNFIQTEHPQTIALILA
                     YLEPNKASYILTKLPKEHQSDVAKRIATMDRTSPETLREVERVLEKKLSTLSSEDYTA
                     AGGVDAIVEILNLADRTTEKSILEALEEENPELAEEIKKKMFVFEDIVMLDNRSIQKV
                     IREVDTQDLTRALKGVDTEVQEKVFQNVSKRAAQALKEDMEYMGPVRIKDVEEAQQKI
                     VAVIRHLEDTGEIIIARGEDDQLIQ"
     gene            complement(1705..>2505)
                     /gene="fliF"
                     /locus_tag="PLW80_02760"
     CDS             complement(1705..>2505)
                     /gene="fliF"
                     /locus_tag="PLW80_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HPB65453.1"
                     /translation="PYDDSVVQPSVTLSTERTSYEYQGTGFNPEGPAGMEGQTAPAYK
                     DLQNMVGKATQTHEIQNEALNKRESFEESSPTVDRVTVSVNIDGVWNWKYNDKGEPAL
                     KADGGIEREYVPVPQADLDAAAALVRDAIGYDRMRGDSVTVRNIQFDRSAQFKAEDAA
                     FLRRQQTNRIVLYSLIGLAFLLVAFIVFRLASREVERRRRIAEEKRALEQQMLRENAI
                     RQAEEQNIEVSMSVEERKRLDLQEHAINMAKEHPEDVAQLIRTWLREE"
BASE COUNT          334 a          841 c          773 g          557 t
ORIGIN      
        1 gcgccgagtc catgccggcc tttgcccgtg ccgtgctcag caacgcgctc gggcgcgcgc
       61 gactcgcccg gcacctgcag cgcgtgctgt tccaggcggg cgaaccctct ttcctcgcgt
      121 cggcccacga cgcgttcgac ctcgagcttg ccctccttga acccttcctc tcggccctgg
      181 tccaagccgt ccttgcaggc acccttggtc acctcgtcga ccctggcctt ggcctgggcc
      241 tcgaaggcct ggatcttggc ctcagcctcg gcgatcgcct tctcggcggc cgactcggcc
      301 tcgcggcgta tcttcagggc ctggtcgttc ttgcgccgga cctcctcgaa ggcggccgcc
      361 tcggccttgg ccacgatgtc ctgcgcctcc gcccgggcgg cggccatcat ggcctccttc
      421 tcgcgttccc agttctggct gaacagctcg gcctcggccc ggagatcgtc tgcggtcggc
      481 ccctcgtact cgagcgcggg cgcgacggcc tccacgtcct cgacgacgat gtcgccgcct
      541 ggggcgtgga gcacgtaggc gctcgagagg ggcacggtct cctgcggcct gaacacggtt
      601 ttagccatac gctacctttt cctactggat cagctgatcg tcctcgccgc gggcgatgat
      661 gatctcgccc gtgtcctcga ggtggcggat taccgcgact atcttctgct gggcctcttc
      721 aacgtccttg atgcgtaccg ggcccatgta ctccatgtcc tccttgagtg cctgggcggc
      781 gcgcttggac acgttctgga acaccttctc ctggacctcg gtgtcgacac ccttgagggc
      841 ccgcgtgagg tcctgggtat cgacctcgcg gatgaccttc tggatcgagc ggttgtccag
      901 catgacgatg tcctcgaaga cgaacatctt cttcttgatc tcctcggcca gctcggggtt
      961 ctcctcctcg agcgcctcga ggatgctctt ctccgtggtg cggtcggcta ggttgagtat
     1021 ctcgacgatg gcgtcgacgc cgcccgcggc ggtatagtcc tcgctcgaca gcgttgacag
     1081 cttcttctcg agcacgcgtt ccacctcgcg cagggtctcc ggcgaggtac gatccatcgt
     1141 cgcgatgcgc ttggccacgt ctgactggtg ctccttgggc aacttggtca ggatgtagct
     1201 ggccttgttc ggctcgaggt aggccaggat cagggcgatg gtctgcgggt gctcagtctg
     1261 gatgaagttg agcaggttgg ccgggtcggt acggcggatg aagtcgaacg gcctgacctg
     1321 gagggagctg gtcaggcggt tgatgacgtc tatcgccttc tgggagccga gggctttctc
     1381 gagcagctcg cgggcatagt cgatgccgcc tgagctgatg aactcgctgg ccatcatcag
     1441 ctcctggaac tccatcagga tggcgtcctt gagctccggc tcgacgttct ccaggcgcgc
     1501 tatctcgaag gtcagcgtct cgatttcatc ctcgcgcagg tgcttgaaga tctcggcgga
     1561 tatctcgcta ccgagggtga tgaggaagat ggcggccttc tggcgaccgt tcaggtcctt
     1621 ggcgctttta ggcttcttgc cggtcgatcc gctcgctccc ggtcccgtcg acttgccctt
     1681 ggctggggcg gcttggggca tctgtcactc ctccctgagc caggtccgga tgagctgagc
     1741 cacgtcctcg ggatgctcct tggccatgtt gatggcgtgc tcctggagat ccagtcgctt
     1801 gcgctcctcc acggacatgg acacctcgat gttctgctcc tccgcctgcc gtatcgcgtt
     1861 ctccctgagc atctgctgct cgagggcccg cttctcctcc gctatcctgc gcctgcgctc
     1921 gacctccctc gaggccagcc tgaagacgat gaacgcgacg agcaggaagg ccaggccgat
     1981 cagcgagtat aacactatcc ggttcgtctg ttggcgccgg aggaaggccg cgtcctcggc
     2041 cttgaactgg gcgctacggt cgaactggat attcctcacg gtgaccgagt cgccgcgcat
     2101 cctatcgtag cctatcgcgt cacggacgag ggccgccgcg gcgtccaagt cggcctgcgg
     2161 caccggcacg tactcgcgct cgatgccacc gtcggccttg agggccggct cgcccttgtc
     2221 attatacttc cagttccaga ctccgtcgat atttaccgac acggtcactc tatcgacggt
     2281 cggagagctc tcctcgaagc tctcgcgctt gttcaaagcc tcgttctgga tctcgtgggt
     2341 ctgggtagcc ttgccgacca tgttctggag gtccttgtag gccggagcgg tctgtccctc
     2401 catgcccgcc gggccctcgg gattgaagcc cgtaccctgg tactcgtaac tcgtccgctc
     2461 cgtcgacagc gtgaccgacg gctgcacgac cgagtcgtcg taggg
//
LOCUS       DAOOLN010000155         2502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_51546_length_2502_cov_2.838578, whole genome shotgun sequence.
ACCESSION   DAOOLN010000155 DAOOLN010000000
VERSION     DAOOLN010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51546_length_2502_cov_2.838578"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..576)
                     /locus_tag="PLW80_02765"
     CDS             complement(79..576)
                     /locus_tag="PLW80_02765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB65454.1"
                     /translation="MIRTATTDDIPRIAEIQIGGWRYAYRGLLSDHELFSERLVVRAV
                     KATAARLEAGCEFVVFDDPADGVVKGFAWHGRCRDDDAADSYEINAIYVQPEFCRGGI
                     GGSMLGFVADYARARGYGELIVWVLDGNAKGLGFYEKHGFIADGKRKLIEKWGRDEIR
                     MVKRL"
     gene            complement(589..1104)
                     /locus_tag="PLW80_02770"
     CDS             complement(589..1104)
                     /locus_tag="PLW80_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65455.1"
                     /translation="MELRRFFSPSTVGLGTAIAAASSALSFLFAFIGLGRPYYASVFM
                     PLIMICLLALAWFMYLRDDGLARRPPASGRPSGASGAPATGAESEPWPAEPVAVGGLD
                     LDGGAAGSAAPYSVLFVPRSSGLVPRAPVAAPAGRRLSVRGACVWAAFELGAASAVLY
                     AATGLGASFFQ"
     gene            complement(1191..1688)
                     /locus_tag="PLW80_02775"
     CDS             complement(1191..1688)
                     /locus_tag="PLW80_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65456.1"
                     /translation="MAKRRVLLVAALVILAALAFAEPVAKDGMIIAPGTLDVNVGIGY
                     GYVWGLDIGGGAEFAIGKFMIGESLPFSYGVAARAGLGIGDTFPLSLAALGTVHFCWG
                     AIEWPDELAWLANVDSYIGLGLQILPGLYIASIGGSSYFINEHFAINVEGGLRASYLG
                     VLYKF"
     gene            complement(1725..>2502)
                     /locus_tag="PLW80_02780"
     CDS             complement(1725..>2502)
                     /locus_tag="PLW80_02780"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HPB65457.1"
                     /translation="FQARFRFNAAVGADGRPVSAAMPGLRSFSLAYSDPKPVDVEPSV
                     AFSGDAVVVEGVPRSSQMVYPDGGKVWCSPTSVSMVLGYWAGDRSPDGAAREARVRAA
                     VAGVYDHVYGGHGNWSFNVAYAGSAPGMEAYMARFEGLDRLEPWLSAGVPLVLSVAWD
                     TGAGRPITAAPVPRSSGHLTTLVGFTARGDAVMHEPASPSNDSVRRVYDRRELESRWL
                     AASGGAAYVIFPSAAGPDDRSRLFASTIGRRLAGIDGMAL"
BASE COUNT          452 a          823 c          892 g          335 t
ORIGIN      
        1 tcggccgcta tcgcgtaggg taagaccgta tcgccgacgt agcgcggcga tacgggtact
       61 gcgacgcggc ggcggccgct agagccgctt gaccatccga atctcatccc tgccccattt
      121 ttcgatgagc ttgcgcttgc cgtcggcgat gaagccgtgc ttctcgtaga agccgagccc
      181 tttggcgttg ccgtccagga cccacacaat cagctctcca tagccccgcg ccctggcgta
      241 gtcagccacg aagccgagca tggagccgcc tatcccgccg cggcagaatt ccggctgcac
      301 gtagatcgcg ttgatctcgt acgagtcggc cgcgtcgtcg tcgcgacagc gaccgtgcca
      361 tgcgaagccc ttgacgacgc cgtccgctgg gtcgtcgaag acgacgaatt cgcagcccgc
      421 ctcgaggcgc gccgccgtcg ccttgacggc ccggacgacc aggcgctcgg agaacagctc
      481 gtggtcggat agcaggcctc ggtacgcgta gcgccagccg cctatctgga tctcggctat
      541 cctgggtatg tcgtccgtag tcgcggtccg tatcatgggt gcttcctact attgaaagaa
      601 gctggcgccg agcccggtcg cggcgtacag aacggccgac gcggcgccca gctcgaaggc
      661 ggcccagacg caagcgcccc ggacgctgag acgacggccg gcgggagcgg cgaccggggc
      721 ccggggtacg aggccactgg aacggggaac gaacaggacg gagtacggcg cggccgagcc
      781 tgcggcacca ccgtccaagt cgaggccgcc gacggctacg ggttcggccg gccacggttc
      841 cgactcggcc ccggtggcgg gcgcgccgga cgcgccggac ggccgccccg aggcgggcgg
      901 cctcctggcc agtccatcgt cccgaaggta catgaaccag gccagggcga ggaggcagat
      961 catgatgagc ggcatgaaaa ctgaggcgta gtacggacgg ccgaggccga tgaacgcgaa
     1021 caggaaggac aaagccgacg aggcggccgc gatggcggtg ccgaggccga ccgtgctcgg
     1081 gctgaagaaa cgccgaagct ccatggagcg gcagtatggt ggcgcggcga gcgattgtca
     1141 ataaaaacgg gcgccaggag gcgcccgttc agtctaccgt gacgcctgga tcagaacttg
     1201 tagagcacgc cgaggtacga ggcgcgcagg ccgccctcaa cgttgatggc gaagtgctcg
     1261 ttgatgaagt agctcgagcc gcctatcgag gcgatataga ggccggggag gatctgcagg
     1321 ccgaggccga tgtagctgtc gacgttggcc agccaggcca gctcgtcggg ccactcgatg
     1381 gcgccccagc agaagtgcac cgtgccgagg gcggcgagcg acaaggggaa ggtatcgccg
     1441 atgccgaggc cggcccgggc agcgacgcca tagctgaagg gcaggctctc gcctatcatg
     1501 aacttgccga tggcgaactc cgccccgccg cctatgtcga ggccccagac atagccgtag
     1561 ccgatgccca cgttcacgtc gagcgtgccg ggagctatga tcatgccgtc cttggcgacc
     1621 ggctcggcga acgcgagagc ggccaggatg acgagcgccg cgaccaggag aactcttctt
     1681 ttagccatac gtacctccga agggatgaag tgaatgtacc atcactatag agccatcccg
     1741 tcgatcccgg caagccgtcg gccgatggtc gaggcgaaga ggcgtgagcg gtcatccggg
     1801 ccggcggccg acgggaagat gacgtacgcc gctccgcccg aggccgccag ccatcgcgat
     1861 tctagctcgc gccggtcgta gacgcggcgg acgctatcgt tcgacggcga ggccggctcg
     1921 tgcatcacgg cgtcgccgcg ggcggtaaag ccgaccagcg tggtcaggtg accggaggat
     1981 ctgggcaccg gcgcggccgt gatggggcgg cccgcgccgg tatcccaggc gaccgacagc
     2041 accaacggca cgccggccga cagccagggc tcgaggcggt cgaggccctc gaagcgggcc
     2101 atataggcct ccatgcccgg cgccgagccg gcgtacgcga cgttgaacga ccagttgccg
     2161 tgcccgccgt agacatggtc gtagacgccg gcgaccgcgg cccggacgcg ggcctcccgg
     2221 gcggccccgt cgggactgcg gtcgccggcc cagtagccga gcaccatcga tacggaggtc
     2281 gggctgcacc agaccttgcc gccgtccgga tagaccatct gcgagctgcg cggcacgccc
     2341 tcgacgacga cggcgtcgcc cgaaaacgcg acggagggct cgacgtcgac cggcttggga
     2401 tctgagtacg cgagcgagaa cgaacggagc ccgggcatgg cggccgagac ggggcggccg
     2461 tcggcgccga ccgccgcgtt gaagcggaag cgcgcttgga ag
//
LOCUS       DAOOLN010000156         2501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_51577_length_2501_cov_2.988962, whole genome shotgun sequence.
ACCESSION   DAOOLN010000156 DAOOLN010000000
VERSION     DAOOLN010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51577_length_2501_cov_2.988962"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="PLW80_02785"
     CDS             complement(<1..1095)
                     /locus_tag="PLW80_02785"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HPB65458.1"
                     /translation="MYDFDSPIDRRGTDSVKWMAGGACHSSIWPDVIPMWVADMDFAS
                     PPAVALAIRRRAEHPVYGYSMKTEAFFHSYLDWMGSRHGLAIEREWLSFSPGIVPAIA
                     TAVRALTEPGDGVAIMPPVYHPFRNMVEKNGRIVVEAPLSRSDGRYGIDLGALDAACS
                     RSRLLVLCSPHNPVGRVWTRDELEAVFDIAERRDVIVFSDEIHGDLVYDDGRMVPSLA
                     LGGGATDRLIAAWAPSKTFNIAGLQASFIVTPDPAIRARFDAESEATGIGSPNCLAQD
                     AVIAAYREGSAWLDEALRHMRGNYDALVSGLEARVPGIRAYPLEGTYLAWLDFRGVGL
                     QGDVGAEIVERAGLWLDAGLRFGSAGAGFAR"
     gene            complement(1099..1479)
                     /locus_tag="PLW80_02790"
     CDS             complement(1099..1479)
                     /locus_tag="PLW80_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPB65459.1"
                     /translation="MSKSVIATTSAPAAIGPYSQAVKVGDLVFCSGQLGLDPATGEFA
                     PGGVRAQAEQAMKNLEALAKAAGSSLDGIVKTTIFLKDMADFPVVNEVYGARFKGDKP
                     ARSTVAVAGLPKGGLVEIEAILSL"
     gene            1655..>2501
                     /locus_tag="PLW80_02795"
     CDS             1655..>2501
                     /locus_tag="PLW80_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPB65460.1"
                     /translation="MIRRVPTFCGKDCGGNACPLLAVLNGGRVERLERNPAGAELKPC
                     PRGYALHREHYAAERLLSPLIADGPRGCGRYREASWDEALSLVADRLGELRARHGPTG
                     LMALGSSGSTGLVHDSQNLLKRFVNACGGGVHPRSNYSNGAAGFALPYLFGAAAGESG
                     WDAKTVRQSRLIVLWGANILEARLGAELGPEVAAAARSGVPVVYIDPRRTMTAKALGA
                     SWIPIRPGTDAAMMLATLRVLFRDGLVDRDGARRLAVGLDELERYVSGGDDGVERSPA
                     WAERIC"
BASE COUNT          410 a          856 c          863 g          372 t
ORIGIN      
        1 acgagcgaag ccggcgccgg ccgaaccgaa gcggaggccc gcatccagcc acaggccggc
       61 ccgctcgacg atctccgcgc cgacgtcgcc ctgaagcccg acgcccctga aatccagcca
      121 ggccaggtac gtgccctcga gcggataggc ccgtatgccg ggaacgcggg cctcgaggcc
      181 ggatacgagg gcgtcatagt tgccgcgcat atggcggagc gcctcatcga gccaggccga
      241 gccctcgcgg tacgccgcta tgaccgcgtc ctgggccagg cagttcggcg agccaatgcc
      301 cgtggcctcg ctctcggcgt cgaatcgagc gcggatggcc ggatcgggcg taacgatgaa
      361 cgacgcctgg aggccggcaa tattgaaggt cttgctcggc gcccaggcgg cgatcaggcg
      421 atcagtcgcg ccgccgccca gggcgagcga cggcaccatg cgcccgtcgt cgtagaccag
      481 gtcgccatgg atctcgtcgg agaacacgat cacgtcgcgg cgctcggcta tgtcgaatac
      541 ggcctcgagc tcgtccctcg tccaaacgcg cccgaccggg ttgtgcgggc tacagagcac
      601 gaggagcctc gaacgggagc aagccgcgtc caaggctccc aggtcgatac cgtagcggcc
      661 gtcggacctg gagagagggg cctcgacgac gatccggccg ttcttctcga ccatgttcct
      721 gaacgggtgg tagaccggcg gcatgatggc gacgccgtcg cccggctcgg tcagcgctcg
      781 aacggccgtc gcgatcgccg ggacgatgcc aggagagaag gacagccact cgcgctcgat
      841 ggctaagccg tggcgcgagc ccatccagtc gaggtacgaa tggaagaacg cctcggtctt
      901 catcgagtag ccgtagaccg gatgctcggc cctccgcctg atcgccaggg cgacggccgg
      961 cggcgaggcg aaatccatgt cggccaccca catcgggatc acgtcgggcc agatcgacga
     1021 atggcaggcg ccgccggcca tccacttgac cgagtcggtg cctctccggt ctatcgggct
     1081 atcgaagtcg tacatggcct acagcgacag tatcgcctcg atctccacga gcccgccctt
     1141 gggtaagccc gcgaccgcga cggtcgatcg ggcgggcttg tcacccttga agcgggcgcc
     1201 gtagacctcg ttgaccaccg ggaagtcggc catgtccttg aggaagatcg tcgtcttgac
     1261 gatgccgtcg agcgagctgc cggcggcctt cgccagggct tcaaggttct tcatcgcctg
     1321 ctccgcctgg gcgcgcacgc cgccgggcgc gaactctccg gtcgccgggt cgaggccaag
     1381 ctggccggag cagaatacca ggtcgccgac cttgacggcc tgcgagtacg ggccgatggc
     1441 cgccggggcc gaggtggtgg ctatgacgct cttgctcatg atgacgctcc ttacggtgta
     1501 gtggctaggc gatggacgcc ttggctcgat gagataaccg gcgcgccggt tccaggcggc
     1561 tagtatgccc cctcccgggt atcgtgtcaa acaaaccccc gaacggcttg actgcggctt
     1621 gactgcgggt cgccgtacgt cgtctactca gggcatgata cggcgagtac cgactttctg
     1681 cggcaaggac tgcggcggca acgcctgccc gctcctggcg gtcttgaacg gcggccgcgt
     1741 cgagcgcctc gagaggaacc ccgccggagc cgagctcaag ccatgcccgc gaggctacgc
     1801 gctccaccga gagcactacg cggcagaacg gctcctctcg ccgctcatcg ccgacggccc
     1861 gcgcggctgt ggtcgctacc gggaggcgag ctgggacgag gcgctgtcgc tggtcgccga
     1921 tcgcctcggc gagctccggg ccagacatgg gccgaccggc ctgatggcgc tcggtagctc
     1981 cggctcgacg ggcctcgtcc acgacagcca gaacctgctc aagcgcttcg tgaacgcctg
     2041 cggcggcggc gtccatccga ggagcaacta ttcaaacggg gcggctggct tcgcgctacc
     2101 gtacctattc ggagccgcgg ccggcgaatc cggctgggac gcgaagacgg tcaggcaaag
     2161 ccgcctgatc gtcctttggg gcgcgaacat actcgaggcc aggctcggcg cggagctcgg
     2221 gcccgaggtc gccgcagccg cccgatcggg cgtgccggtc gtctatatcg acccgcgcag
     2281 gacgatgacg gccaaagccc tcggggcgag ctggataccg atacggccgg gcaccgacgc
     2341 ggccatgatg ctcgcgacgc tgcgcgtcct attccgggac ggcctcgtcg accgcgatgg
     2401 cgcccgccgc ctggcggtcg ggctcgacga gctggaacgc tacgtctcag gaggcgatga
     2461 cggcgtcgag cgctcgcccg cctgggccga gcgtatctgc g
//
LOCUS       DAOOLN010000157         2498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_51653_length_2498_cov_3.314368, whole genome shotgun sequence.
ACCESSION   DAOOLN010000157 DAOOLN010000000
VERSION     DAOOLN010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51653_length_2498_cov_3.314368"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PLW80_02800"
     CDS             <1..254
                     /locus_tag="PLW80_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65461.1"
                     /translation="IIAPMRLLIDFFPILLFYTRFASDAARYVRNGFDATYAPWPFKN
                     EQSYEPSDDEQWKNVLTAAGLSVAFAALDALVLWRVQLE"
     gene            315..1385
                     /locus_tag="PLW80_02805"
     CDS             315..1385
                     /locus_tag="PLW80_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPB65462.1"
                     /translation="MHRQAEARDATVLAVDVGGTNTSCAVVDVRDGYLTTTFERRYST
                     KAEPSLYEPLGRFIDEASSAGVALPELLCVSGAGPVVDRRIALTNAPWGIDGDGLSER
                     LGLPTYVINDFSAIAWGVLLLDPSDPNELAQLSAPDGAAPDPYGPVVVIGAGTGLGFG
                     YALRDDGSVRVYPSEGGHVCLPVYDDESRAFSRWLEARYGFAAGAEAGVSGQGIAAIF
                     EFLSASEPSLSEAARAALSAPAEDRPALVSTAADSGDALCLRVMDMFVRLYARVAADA
                     ASVFMPSGGIYLAGGIAAKNLARFTEGDRFMRSFGQGYREHVRAIAARTPVFVVKDYA
                     ISMYGAANAAIHLADGSRRRPC"
     gene            1379..>2498
                     /locus_tag="PLW80_02810"
     CDS             1379..>2498
                     /locus_tag="PLW80_02810"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HPB65463.1"
                     /translation="MLSDALRSDMVAKGVDVETTLSILADLNAGLYDGQKAVQATGVP
                     PVDGASIVPSAKDMAYRIGADEALSRLSDLGLELPDGARADGDSLLFDRAALEDLGER
                     LYPVAAWGVLNGGSATSYADRKKNAALGPGVFEAIRSGFELLAPLCDGRPKGVTPAYI
                     NPDGSPGERFLLLKMRAALLRAERYVERFGTPARPALPFFQMTSDGTDAQLADAYAAY
                     AGHPWIAALIERAGTDPTRPRSARQPLLAAFTHSADGRPRRVFDRAYGRQDSAIALPG
                     GHGQSFRVLADVYRGLLADGYRYAWIGNVDNVGYYPDPAELAVMALSGAEAAFEFSFR
                     TPVDIKGGILITTAEGGRTVADIGQAISFERVQELEAAG"
BASE COUNT          394 a          872 c          813 g          419 t
ORIGIN      
        1 ggataatcgc gcccatgcga ctgctgatcg acttcttccc catcctgctc ttctatacgc
       61 gcttcgcgag cgacgccgct cgctacgtac ggaacggttt cgacgcgacg tacgcgccgt
      121 ggccgttcaa gaacgagcaa tcctacgagc cgagcgacga cgagcaatgg aagaacgtat
      181 tgacggccgc cggcctgtcc gtagcgttcg cggcgttgga cgcgctcgta ctctggcgcg
      241 ttcagctgga gtagccgatg gcgcagcgac ccggtactta acagggtagg ctgacctgca
      301 ttagtattgc gggtatgcat agacaagcgg aagcgcgcga cgcgacggtg ctggccgtcg
      361 acgtcggcgg gacgaatacc agttgcgccg tcgtcgacgt ccgtgacggc tacttaacga
      421 cgactttcga gcgtcgctat tcgactaaag ccgagccgtc gctgtacgag ccgctcggcc
      481 gctttatcga cgaggcttcc tccgccggag tagccctgcc cgagctcctc tgcgtctccg
      541 gcgccggacc cgtcgtcgac cgccgcatag cgcttaccaa cgcgccctgg ggcatcgacg
      601 gggacggcct ctccgagcgc ctcgggctgc cgacctacgt catcaacgat ttcagcgcca
      661 tcgcctgggg cgtgctactc ctcgatccaa gcgacccgaa cgagcttgcc cagctttcag
      721 ctcccgacgg cgccgctccc gatccgtatg gcccggtcgt ggtcatcggc gcgggtaccg
      781 ggttaggctt cggatacgcg ctacgcgacg acggatccgt cagggtctat ccgtccgagg
      841 gcgggcacgt ctgcctaccg gtctacgacg acgagtcgcg cgcgttctcg cgctggctcg
      901 aggctcgcta cggcttcgca gccggcgccg aggccggcgt ctccggacag ggcatcgccg
      961 ccatcttcga gttcctgtcc gcctccgagc ccagcctgtc cgaagccgcg cgcgcggccc
     1021 tatccgcgcc ggccgaagat aggccggccc tcgtgtccac cgccgccgac tcaggcgacg
     1081 cgttatgcct acgggttatg gatatgttcg tgcgactgta cgcgcgagtc gcggccgacg
     1141 ccgcctcggt cttcatgccg tcaggaggca tctacctggc aggcggcatc gccgccaaga
     1201 acctggcgcg atttaccgag ggcgatcgct tcatgcgcag cttcggccag ggctatcggg
     1261 agcacgtccg ggcgatcgcg gctaggacgc cggtcttcgt cgtcaaggac tacgctatat
     1321 cgatgtacgg ggcggcgaac gccgcgatcc acttggccga cggatcgaga cggcggccgt
     1381 gctgagcgac gcgctgcgat ctgacatggt cgccaaggga gtcgacgtcg agacgaccct
     1441 gtccatcttg gccgacctga acgcgggcct ctatgacggt cagaaggccg tgcaggctac
     1501 cggcgtgccg ccagtcgacg gcgcatccat cgtgccctcc gccaaggaca tggcctacag
     1561 gataggcgct gacgaggcgc tgagtcggct ctcggacctc ggcctcgagc ttcccgatgg
     1621 cgcgcgagcc gacggcgaca gcctgctgtt cgaccgggcc gccctcgagg acctcggaga
     1681 gcggctgtac ccagtcgccg cctggggcgt actcaacggc ggctcggcga cgagctacgc
     1741 cgaccgcaag aagaacgccg ccctcggccc cggcgtgttc gaagcgatac ggtcgggctt
     1801 cgagctcctg gcgcctctat gcgacggcag gcccaagggc gtcaccccgg cctacatcaa
     1861 cccggacggt agccccggcg agagattcct gctcctgaag atgcgggcgg ccctcctgcg
     1921 cgccgaacgc tacgtcgagc gcttcgggac gccggcgcgg cctgcgctcc cgttcttcca
     1981 gatgacgagc gacggcaccg acgcccagct cgccgacgcc tatgccgcgt acgccgggca
     2041 tccttggatc gcagcgctca tagagcgggc cggcaccgat ccgacgcggc cgcgctcggc
     2101 gcgtcaaccg ctactggctg cttttacgca ttcagccgat gggcgtcccc gccgcgtgtt
     2161 cgatcgcgcc tacggaaggc aggacagcgc catcgccctg cccggcggac acggccagag
     2221 cttccgtgta ctcgccgacg tgtatcgcgg gcttctggcg gacggctatc gctacgcatg
     2281 gataggcaac gtcgataacg tcggctacta tccggacccg gctgagctgg ccgtcatggc
     2341 cctgtccggg gccgaggcgg cattcgagtt ttcgttccgc accccggtcg acatcaaggg
     2401 cggcatcctc atcacgacgg ccgaaggcgg gcgcacggta gctgatatcg gccaagccat
     2461 ctccttcgag cgcgtccagg aactggaggc cgccggct
//
LOCUS       DAOOLN010000158         2492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_51819_length_2492_cov_2.674600, whole genome shotgun sequence.
ACCESSION   DAOOLN010000158 DAOOLN010000000
VERSION     DAOOLN010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51819_length_2492_cov_2.674600"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PLW80_02815"
     CDS             <1..217
                     /locus_tag="PLW80_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65464.1"
                     /translation="GELLRDYNDGRSKNLYCIVANDAPLDELETLIDSARSLSGDLDL
                     KEKARRVRSEIQALERRLGFEFKLRRP"
     gene            214..1383
                     /locus_tag="PLW80_02820"
     CDS             214..1383
                     /locus_tag="PLW80_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65465.1"
                     /translation="MNRRTVIALGYLGLLLSATAVSAAQGPSPESDAVTASALSPPAR
                     LEYYGELGCSHCDTFMDRDLPAAEAASGVRVELEAFDILSEAGYSRCEARLAELGYGF
                     RIFPVLIVGRSAYQGNAAIEANLLPELTSYAETGSFRPRFDDGMAHDAGRGAPVWTVP
                     AILAAGLIDGVNPCAFTTLLFFMSYLGLRSGSRWRAAAAGLAFAAGVFLAYFAIGYGV
                     LNVFRLGGRLQGFRLALKLTVSVLTAAFFAVTVRDIAALLRDRSASPVMMLPARLRTD
                     INAMIRAGTGSPALILGAFVSGVVVSVLELACTGQIYFPAISAMVQAGVSGSGLLYLT
                     LYNLAFVLPLLAVLAASLLGLRQEALRSFFKRHLVASKAAQAVLFAGLSIAIWLA"
     gene            1446..2345
                     /locus_tag="PLW80_02825"
     CDS             1446..2345
                     /locus_tag="PLW80_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65466.1"
                     /translation="MESIEERQRRTKRHLGDRYDGRRVRSLNPFYQITPYIMPTRVDA
                     HDYFEDRIEISHTEAWLRRQRDAGYPEMGYLHVFIAAYVRMISQKPRLNRFIAGKKIF
                     ARNEITFSLALKKRLHEDSPETTVKMRFNPEDTIYDVMRTVNEAIGQNKETAATNATD
                     KVARLIMLCPGFLVSMLIGLLRVLDFYGIMPRVIHRASPFHTSVFITDLGSLGIKPIY
                     HHLYNFGTTSIFVAFGAKERQREILKDGTIVERRYIGMKVVNDERICDGHYYASAFKL
                     LLSYFKDPSQLERPPEAVVRDQD"
BASE COUNT          443 a          812 c          742 g          495 t
ORIGIN      
        1 cggggagctc ctccgcgatt acaatgacgg gcgctccaag aacctgtact gcatcgtcgc
       61 gaacgacgcc ccgctcgacg agctcgagac tctgatcgat agcgccagaa gcctatccgg
      121 cgacctggac ctcaaggaga aggcgaggcg ggtacgttcc gagatacagg ctcttgaacg
      181 tcgcctcggt ttcgagttca agctgaggcg gccgtgaatc gacgtacggt catcgctctc
      241 ggctatctgg ggcttcttct gtcggccacg gccgtctcgg ctgcccaggg accgtcgccc
      301 gagtccgatg ccgtcaccgc gtcggcctta agcccgcccg ctcgcctcga gtactacggc
      361 gagctcggtt gctcgcactg cgataccttc atggataggg acctgccggc ggccgaggct
      421 gcgagcgggg tgcgggtaga gctcgaagcg ttcgacatcc tgtcggaagc gggctactcc
      481 cgttgcgagg ccaggctcgc cgagttgggc tacggattcc gcatctttcc ggtactgatc
      541 gtcggtcggt ccgcctatca gggtaacgcc gccatcgagg ccaacctgct accggagctg
      601 accagctacg cggagacggg ctcgttcagg cccaggttcg acgacggaat ggcgcatgac
      661 gcaggtcgcg gcgcgccggt ctggaccgtt ccggccatac tcgccgccgg cctgatcgac
      721 ggcgtcaatc catgcgcctt cacgacgcta ctgttcttca tgtcgtacct ggggctgcgc
      781 tccggctccc gctggcgagc ggccgccgct gggctggcct tcgccgcggg cgtgttcctg
      841 gcgtacttcg ctatcggcta cggcgtactc aacgtgttca ggctcggcgg caggcttcaa
      901 ggattccgcc tggccctcaa gcttaccgtc agcgtgctga ccgcggcctt cttcgccgtg
      961 accgtcaggg atatcgcagc gctactgcgg gaccgctcgg cttcgccggt catgatgctg
     1021 cccgcccggt tacgcacgga catcaatgcg atgatccgcg ccggaacggg gagccccgcg
     1081 ctgatcctcg gcgcgttcgt ctcgggagtc gtcgtctcgg tgctcgagct cgcctgtacc
     1141 ggtcagatat atttcccggc catatcggcc atggtccagg cgggagtctc gggctccggc
     1201 ctcctgtacc tgaccctgta caacctggcg ttcgtcttgc ccctcctggc ggtcctcgcc
     1261 gcctcgctcc tgggcctgcg ccaggaagcg ctgagatctt tcttcaagag gcatctcgtc
     1321 gcgtccaagg ccgcccaggc tgtgttgttc gcggggcttt ctatcgcgat atggctggcc
     1381 tgaccaaggt cgccatctag taaacgagcc tccgagcgag tagaatggcc tctttcggag
     1441 gatcgatgga atctattgaa gagcgccaaa gacggaccaa gcggcacctc ggcgaccgat
     1501 acgacggcag gagggttcgt tccctcaatc cgttctacca gatcacgccc tatatcatgc
     1561 ccaccagggt cgacgcccat gattatttcg aagataggat tgagatatcg cacaccgagg
     1621 cctggctgag gcgccagcgc gacgccggat atccagagat gggctatctg catgtcttta
     1681 tcgcggccta cgtgcgcatg atctcccaga agccgaggct caaccgcttc atagccggca
     1741 agaagatttt cgcgcgcaac gagatcacct tctcgctggc tctcaagaag cggctccacg
     1801 aggacagccc ggaaaccacg gtaaagatgc gcttcaaccc agaagacacc atctatgacg
     1861 tgatgcggac cgtcaatgag gcgataggtc agaacaagga aaccgccgcg accaacgcca
     1921 ccgacaaggt cgccaggttg atcatgctct gccctggctt cctcgttagc atgctgatag
     1981 gactattacg cgtcctggat ttctacggga tcatgcccag ggtaatccac cgggccagcc
     2041 cattccatac cagcgtattc ataaccgacc tgggctcgct cggtatcaag ccaatctacc
     2101 atcacctgta caatttcggc accacgtcta tcttcgtcgc cttcggagct aaggagaggc
     2161 aacgagagat cctgaaagat ggtacgatcg tagagcgccg ctacatcggc atgaaggtcg
     2221 tcaacgacga gcgcatctgc gacggccatt actacgccag cgcgttcaag ctgctcctaa
     2281 gctacttcaa ggacccgagc cagcttgagc gaccgcccga ggcggtcgtc agggaccagg
     2341 attagcccag gtcggttccg gttgtaagct tccctttctt tggagccgtg ggctcggtcg
     2401 gcgcctcgtc agccttcgcg gtagccttcg cgtcagccgc gcccgccatg tcgccgagcg
     2461 cggtcttctc cttctccagc cgcttgagcc gg
//
LOCUS       DAOOLN010000159         2488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_51926_length_2488_cov_3.266749, whole genome shotgun sequence.
ACCESSION   DAOOLN010000159 DAOOLN010000000
VERSION     DAOOLN010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51926_length_2488_cov_3.266749"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..646)
                     /locus_tag="PLW80_02830"
     CDS             complement(104..646)
                     /locus_tag="PLW80_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65467.1"
                     /translation="MRRPSIIGFVAVLLTAALASASAQEWGVSVGSWSFAGGGPGPGA
                     YACAGLSAGLGQRLELEAFGVARATPAPFGDALAGAILGFSVFGPRRTTYFNMVLDLG
                     FLQTVVEDGIPGLGPSYAMLRLSPLVVGTPYYGHRDRIFSLGLLYDVRSGALSATWNF
                     LIVHWYPGSHAWLPAGDRER"
     gene            complement(643..1197)
                     /locus_tag="PLW80_02835"
     CDS             complement(643..1197)
                     /locus_tag="PLW80_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65468.1"
                     /translation="MKLVRTFACAALLAAAALPTSAEPRSPWLGGVLFSTRGFSEDSS
                     HAAGEYLDLGLFVDPFDFAVLRPSASLRLIVPLFPFDAGRIEGAAGLDLELFGLRKHP
                     LRAIMAQASAYAPALSAARSFSFVEPLSGEWAVGAHPIRLRTGDARYSFLSPELRFGP
                     GFEPAGWSLKLFEFSYLIFAEARR"
     gene            complement(1201..>2488)
                     /locus_tag="PLW80_02840"
     CDS             complement(1201..>2488)
                     /locus_tag="PLW80_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HPB65469.1"
                     /translation="GAARTAAAAARTAAAAIVALALLSASGCASYGRLERYRETYPDA
                     GAFLESRGVPAFGAAAPALYMDGGAWFERAMELIAGAEDYILISSFLITDIDRQAAIF
                     DALAEQMTDGVRVYMIVDSASYYRTYPMDAEPIPASTPLARNLGIPLIEYNPIRGRRI
                     FTLLGLLDRDHRKFWVVDGRIVVTGGQNVDYDSLRDVGNGGCVDAMIEYESPGAAAFL
                     RDSFVRTWNAYSLDPLDPDDFAVGDADTPYRLSVIDQGLRSPGLITDMFDGFMAFASE
                     ELWLVQCYAYLTPSLLDRVRFAVDRGVRVNVVLSREHVTARALYGSYYGMLDLLDAGA
                     SVYLYESDVGSLLHFKLIMADGELASVGSANYNLRSQTTSRELSFIVSDQESLAMIRA
                     ELDALLASCRPVGREEARRYRGLPYLLQNLLMQFWG"
BASE COUNT          472 a          821 c          880 g          315 t
ORIGIN      
        1 caggtcgtcg gccgagacgc gggccggtag cgaggcgagg acggtcagga cagccgcgag
       61 cgcggctatc cggcccgagg cgcttgtatg tacgagcatg gcgttacctt tcgcggtcgc
      121 cggcgggcag ccacgcgtgg ctacccgggt accagtggac gatgaggaaa ttccaggtag
      181 ccgacagggc gccggagcgg acgtcgtaca ggagccccag cgagaagatg cggtcgcggt
      241 ggccgtagta cggcgtgccg acgaccagcg gcgacagcct gagcatcgcg tacgacgggc
      301 ccagaccggg aataccatcc tcgacgacgg tctgcaggaa acccaggtcg agaaccatgt
      361 tgaagtaggt ggtccgccgg ggcccgaaca ccgagaagcc gaggatggcg ccggccagcg
      421 cgtcgccgaa cggcgccggc gtggcccgcg cgacgccgaa ggcctcgagc tccagtcgct
      481 ggccgagccc ggccgacagg ccggcgcagg cgtaggcgcc gggccccgga ccgccgccgg
      541 cgaacgacca cgagccgacc gagacgcccc attcctgcgc ggacgcgctt gccagcgccg
      601 ccgtcaggag aacagccaca aacccgatga tagatggccg cctcatcgcc gcgcctccgc
      661 aaagatcagg tatgagaact cgaacagctt gaggctccag ccggccggct cgaaccccgg
      721 gccgaagcgc agctccggcg acaggaagct atagcgcgcg tcaccggtcc tgagcctgat
      781 aggatgagcg ccgacggccc actccccgct cagaggctcg acgaaggaga agctcctggc
      841 ggccgacagc gccggcgcgt aggcgctggc ctgggccatg atcgcgcgga gcggatgctt
      901 cctcaggccg aacagctcca ggtccaggcc agcggcgccc tcgatccgcc ccgcgtcgaa
      961 cgggaacagg gggacgatca gccggagaga ggctgacggc ctcaggacgg cgaagtcgaa
     1021 cggatcgacg aagaggccca ggtctagata ctctccggcc gcgtgggacg agtcctcgga
     1081 aaagcccctg gtcgagaata ggacgccgcc gagccagggc gagcgcggtt cggccgaggt
     1141 tggcagggcc gccgccgcga gcaaggcggc gcaggcgaac gtacgtacca gcttcatagt
     1201 ctaaccccag aactgcataa gcaggttttg caggagatac ggcaggcccc ggtagcgtct
     1261 ggcctcctcg cggccgaccg gccggcagga ggcgagcaga gcatcgagct cggcgcgtat
     1321 catagccagc gactcctggt cggagacgat gaaggacagc tcccgcgagg tcgtctgcga
     1381 acgcaggttg tagttcgccg aaccgaccga agccagctcc ccgtccgcca tgatcagctt
     1441 aaaatggagc agggagccga cgtcagactc gtagaggtag accgaggcgc ccgcgtccag
     1501 aaggtcgagc atgccgtagt acgacccgta cagcgcccgc gccgtcacgt gctcgcgcga
     1561 cagcaccacg ttgacgcgca cgccgcggtc gaccgcgaag cgcaccctgt cgagcaggga
     1621 cggcgtcagg taggcgtagc actgcacgag ccacagctcc tcggacgcga aagccatgaa
     1681 gccgtcgaac atgtcggtga tgagccccgg gctacgcagg ccctggtcga tgaccgacaa
     1741 ccgatacggc gtgtcggcgt cgccgacggc gaagtcgtcg ggatcgagcg ggtcgaggga
     1801 atacgcgttc caggtacgca cgaagctgtc gcgcaggaag gcggccgctc ccggcgactc
     1861 gtactcgatc attgcgtcga cgcagccccc gttccctaca tcgcgcagcg agtcatagtc
     1921 gacgttctgg ccgccggtga ccacgatccg tccatcgacg acccagaact tgcggtgatc
     1981 gcggtcgagc aggccgagca atgtgaagat gcgccggccg cgtatggggt tgtactcgat
     2041 cagcggtata ccgaggttcc tggccaacgg cgtcgaggcc gggatgggct cggcgtccat
     2101 cggataggtg cggtagtacg acgccgagtc gacgatcata tacacgcgga cgccgtcagt
     2161 catctgctcg gccagcgcgt cgaagatggc ggcctgccgg tcgatatcgg ttatgaggaa
     2221 cgacgatatg agtatgtagt cctcggcgcc ggctatcagc tccatcgcgc gctcgaacca
     2281 agcgccgccg tccatgtaca gagccggcgc ggcggccccg aaggcgggca cgccgcgcga
     2341 ctccaagaag gcgccggcgt ccggataggt ctcccggtag cgctcgagcc taccgtagct
     2401 ggcgcagccg gaggccgata gcagggcgag cgcgacgatg gccgcggcgg ccgtccgggc
     2461 ggccgcggcg gccgtccggg cggccccg
//
LOCUS       DAOOLN010000160         2480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_52168_length_2480_cov_3.935670, whole genome shotgun sequence.
ACCESSION   DAOOLN010000160 DAOOLN010000000
VERSION     DAOOLN010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52168_length_2480_cov_3.935670"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1662)
                     /locus_tag="PLW80_02845"
     CDS             complement(<1..1662)
                     /locus_tag="PLW80_02845"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HPB65470.1"
                     /translation="MMRYPAGDERLFGISVPLGSIKVHGGWRVGEYPDLVPFAGLCKE
                     LGARLLQLLPLNDTGGQSSPYSALSAFALHPLYIRVRDLPEAAAAPGAVEELESFAAK
                     ATSGERFPYGACLDAKLAALRAVYKASADSIAADAGLASFESDRPWLRTYAVYRRLKS
                     SYGERAWRSWPDHGRPTRGEIDTLWDDPSARREQRFHAWMQYRAAEQFGAAAEAVAGM
                     GLVLLGDIPILMNDDSADVWADRGFFDDSLRAGAPPDMYSAVGQNWGFPIYDWAAMAE
                     DGYSFWRARVAEAARYYSAYRIDHVLGFFRIWALGDRDETGCLGRFVPGPELGADELT
                     RSGFSEARLRWLSEPHIPGAELRVAVPATEAASVVDAALERIGDEDLFRFKRSIRGEA
                     DIGALPLGGATRDYLKDRWRDRALLAIGGGRFAPTWSYRGTRAWASLSDGERSVLEAM
                     LGIASASAELDWEDRGRRLLSMLGSASDMLPCAEDLGAVPDCVPRVLSELGIPGLRVP
                     RWMRRWDEPGQPFMPLSEYGELSACTPSVHDTSTLRGWWEREDGRD"
     gene            complement(1659..>2480)
                     /locus_tag="PLW80_02850"
     CDS             complement(1659..>2480)
                     /locus_tag="PLW80_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HPB65471.1"
                     /translation="CALELADWAREHGSACFLVAHVTKDGMIAGPKAAEHTVDVVLMF
                     EQSETDARFLRSAKNRFGSTDEVGLFTMTERGLEEQTDPSELFLVRRAGEAPAGAAVA
                     ATWEGTRALLVEIQALTVPGKASLSRVYSERVEPARVARVAAVLEKHAGLGLSDQDLY
                     VNVSGGMRLSEPGIDLALAAAIYSARTGLALPPSAALAGELSLAGEVRPVRRMSGRVR
                     AARAMGFERVVGAASIRGEAAVRGEADDPDSWERVSTIGATIRALFGSGAKNGGR"
BASE COUNT          400 a          907 c          809 g          364 t
ORIGIN      
        1 gtccctgccg tcctcccgtt cccaccagcc gcgaagcgtc gaggtatcgt ggacgctcgg
       61 cgtgcaggcg gatagctccc cgtattcgga cagcggcatg aacggctggc ccggctcgtc
      121 ccatcgcctc atccagcgcg gtacccgtag cccgggtatg ccgagttcgg agagcaccct
      181 cggcacgcag tcgggaacgg cgccgagatc ctctgcgcag ggcaacatgt cggaggccga
      241 gccgagcatg gacaggagcc ggcggccgcg atcctcccag tcgagctcgg ccgacgccga
      301 agcgatgccg agcatggcct cgagcaccga ccgctcgccg tccgagaggc tcgcccaggc
      361 cctggttccg cgatatgacc aggttggcgc gaagcgtcct ccgcctatgg ccagcagagc
      421 cctatcacgc catctgtcct tgagataatc cctggtggct ccgcccagag gcagggcgcc
      481 tatatctgcc tcgccccgta tcgatcgctt gaacctgaat aggtcttcgt cgccgatgcg
      541 ctcgagcgcc gcatcgacga cgctcgcggc ctccgttgct ggtacggcaa ctcggagctc
      601 ggcgccgggt atatgcggct cggacagcca gcgtagcctt gcttccgaga agccagaccg
      661 cgtcagctcg tccgccccga gctcgggtcc gggtacgaag cggccgaggc agccagtctc
      721 gtcccggtcg ccgagcgccc atatcctgaa gaacccgaga acgtggtcga tgcggtaggc
      781 cgagtagtac ctggcggcct ccgcgacgcg cgctctccag aaggaatagc cgtcctccgc
      841 catggcggcc cagtcataga tcgggaagcc ccaattctgg ccgaccgccg aatacatgtc
      901 cggaggcgcg ccggcccgga gcgaatcgtc gaagaaaccg cgatccgccc agacgtcggc
      961 cgagtcgtcg ttcatcagga tgggtatgtc cccgaggagg acgaggccca ttccggccac
     1021 tgcctcggct gccgcgccga actgttcggc ggcccgatac tgcatccagg cgtggaagcg
     1081 ttgctcacgc ctagcggagg ggtcgtccca caacgtgtcg atctcgcctc gggtcggccg
     1141 cccgtggtcg ggccacgagc gccaggcccg ctcgccatag gacgacttaa gccgccggta
     1201 gacggcgtag gtacgcagcc acgggcgatc gctctcgaag gaggccaagc cggcgtcagc
     1261 ggctatgcta tcggccgacg ccttgtagac ggccctgagc gctgccagct tggcgtcgag
     1321 gcacgcgccg tatgggaaac gctcgcccga ggtcgccttg gcggcgaacg actccagctc
     1381 ctcgactgcc ccaggcgccg cggccgcctc cggcaggtcc cgaaccctga tgtagagcgg
     1441 gtgtagcgcg aaggccgaca gcgcggagta gggcgagctc tggccgccgg tgtcgttgag
     1501 cggaagcagc tggagcagcc tcgcgccgag ctccttgcat agcccggcga acggcacgag
     1561 gtccgggtac tcgccgaccc tccagccgcc gtgcaccttg atcgacccga gcggaaccga
     1621 gatcccgaac aatcgctcgt cgccggcggg gtaacgcatc atcgtcctcc gttcttggcg
     1681 cccgagccga agagggcccg tatcgtcgcg cctatcgtcg agacccgttc ccaagagtcc
     1741 gggtcgtcag cctcgccccg taccgcggcc tcgccccgaa tggaggcggc gccgaccacc
     1801 cgctcgaacc ccatcgccct ggccgcccga acgcggccgg acatgcgccg caccggccgg
     1861 acctcgcccg cgagcgacag ctccccggcg agcgcagcgg acggcggcaa cgccagcccc
     1921 gtacgcgccg agtagatcgc ggccgcgagc gccaggtcta tgcccggttc cgataggcgc
     1981 atgccgcccg agacgttgac gtacaggtcc tggtctgaaa ggccgaggcc cgcgtgcttc
     2041 tccagcacgg cggccacgcg cgccacccgc gccggctcga cgcgctcgga gtagacgcgt
     2101 gacaaagacg ccttgcccgg cacggtcaac gcctggatct ccaccagcag ggcgcgcgtc
     2161 ccctcccagg tagccgccac ggccgcaccc gccggcgcct cgccagctcg gcgcacgagg
     2221 aacagctcgg acgggtcggt ctgttcttcg agcccacgct cggtcatcgt gaacagccct
     2281 acctcgtcgg tcgagccgaa tcggtttttg gccgagcgta ggaaacgcgc gtcggtctcg
     2341 ctctgctcga acatcagcac cacgtcgacg gtatgctccg cggccttggg ccccgctatc
     2401 attccgtcct tcgtcacgtg ggcgacgagg aaacaggcgc taccgtgctc cctggcccag
     2461 tcggccagct cgagggcgca
//
LOCUS       DAOOLN010000161         2474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_52346_length_2474_cov_3.379909, whole genome shotgun sequence.
ACCESSION   DAOOLN010000161 DAOOLN010000000
VERSION     DAOOLN010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52346_length_2474_cov_3.379909"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PLW80_02855"
     CDS             complement(<1..408)
                     /locus_tag="PLW80_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPB65472.1"
                     /translation="MTNTKGAVTGVNGNMVSVRVDGTVSMNEVAYIVVGGKRLKSEII
                     RVQGDIVQTQVFEMTKGIKVGDPVEFTGEMLAVELGPGLLGKIYDGLQNPLPEVAAQT
                     GYFLEPGVYLEALPRNVKWSWTPVAKAGDRVERA"
     gene            complement(405..989)
                     /locus_tag="PLW80_02860"
     CDS             complement(405..989)
                     /locus_tag="PLW80_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65473.1"
                     /translation="MSSYYYFVATLPSLQAGASAPPLSHAEFLERSSRFLSEKDMAVL
                     EGARLFIPEDGSFPTAAGSSPLLARYYRWELSLRNELARLRAGRLQKPVEKHVKPGEP
                     EWDGLKGAQAAMQADDPLQGELLIERERWSFVESLAVGRFFDLEYLVSYALMLQALER
                     RARFSAQRGQEGYETVYRSVLETADYRDESGESK"
     gene            complement(997..1617)
                     /locus_tag="PLW80_02865"
     CDS             complement(997..1617)
                     /locus_tag="PLW80_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HPB65474.1"
                     /translation="MDIRVQELLEKIKHDGVEHAEAEAAKILADAEKKRADVIAAAEK
                     EAKAIVAKAESDAARAAESGRAALTQASRDLILAFSGEVQNLLAELVKAEVEAAFDAG
                     TVKKALPAILEAWSKDGRDDLSALLPAKDLKELEAFFKDRLAASFKKGVEFKPLKDAK
                     AGFRIVEKGGAAYYDFSAQAVAEMLGSYLNARLAAIATASVAEAAK"
     gene            complement(1657..2016)
                     /locus_tag="PLW80_02870"
     CDS             complement(1657..2016)
                     /locus_tag="PLW80_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65475.1"
                     /translation="MSSEARHFAKLFRVELDDLIDEIGLLVDRMENRYERGELTEYVH
                     LENAAFYKRMSDSLLKFAGIVDGIDPSIYKCMADIEADLLARSRDFTIKLEDPEAVHI
                     LLRRKLDKVKKFISSDE"
     gene            complement(2340..2411)
                     /locus_tag="PLW80_02875"
     tRNA            complement(2340..2411)
                     /locus_tag="PLW80_02875"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2376..2378),aa:Gly,seq:tcc)
BASE COUNT          444 a          814 c          705 g          511 t
ORIGIN      
        1 ggcgcgctcc accctgtcgc cggccttggc taccggcgtc caggaccact ttacgttcct
       61 cggaagcgcc tccaggtaca cgccgggctc gaggaagtag ccggtctggg ccgcgacctc
      121 gggcagcggg ttctggagcc cgtcgtagat cttgccgagc aggcccggcc cgagctcgac
      181 cgcgagcatc tcgccggtga attcgaccgg gtcgccgacc ttgatgccct tggtcatctc
      241 gaagacctgc gtctggacga tatcgccctg gacgcggatg atctcggact tgagccgctt
      301 accgccgacg acgatatagg cgacctcgtt catcgaaacg gtgccgtcga cgcggacgct
      361 gaccatgttg ccgttaacgc cggtgaccgc ccctttggtg tttgtcattt gctctctccg
      421 gattcatcgc ggtagtccgc ggtttcaagg acggaccggt aaacggtctc gtagccttcc
      481 tggcctcgct gcgcggagaa gcgagcccgg cgctcgagcg cctgcagcat caacgcgtac
      541 gacacgaggt actctagatc gaagaaccga cccacggcga gcgactcgac gaacgaccat
      601 cgctcccgct cgatcaatag ctcgccctgg agcggatcgt ccgcctgcat cgcggcctgg
      661 gctcccttga gcccgtccca ctccggctcg cccggcttca cgtgtttctc gaccggtttc
      721 tgtagcctcc cggcgcgaag tctcgccagc tcgttgcgca atgacagctc ccagcgatag
      781 taccttgcca gtagcggcga cgagccggcc gccgtcggaa aggagccgtc ctctggaatg
      841 aagagcctgg ccccctcgag cacggccata tccttctcgg acaggaagcg cgaggagcgt
      901 tccaggaact cggcgtggga taacggcgga gccgaggcgc cggcctgcag cgacggtagc
      961 gtcgccacga aataatagta ggaagacatg gcggccctac ttcgccgcct ccgcgacgga
     1021 tgcggtcgcg atagcggcca atcgggcgtt caggtacgaa ccgagcatct cggcgacggc
     1081 ctgggccgag aagtcgtagt aggcggcccc gcccttctcc acgatcctga acccggcctt
     1141 agcgtccttg agcggcttga actcgacgcc cttcttgaac gaggcggcga gcctgtcctt
     1201 gaagaaggcc tcgagctcct tgaggtcctt ggcgggcagc agcgccgaca ggtcgtcgcg
     1261 gccgtccttg gaccaggcct cgaggatagc cggcagggcc ttcttgacgg tccccgcgtc
     1321 gaacgcggcc tcgacctcgg ccttgaccag ctcggcgagc aggttctgga cctcgcccga
     1381 gaaggcgagg atcagatccc gcgacgcctg cgtcagcgcc gccctgccgg actcagcggc
     1441 cctggccgcg tcggactctg ccttggcgac tatggccttg gcttccttct cggcggccgc
     1501 gatgacgtcg gcccgcttct tctcggcatc ggcgagtatc ttcgccgcct ctgcctcggc
     1561 atgttccacg ccgtcgtgct tgatcttctc cagtagttcc tgcacccgaa tatccatggc
     1621 atcctctttc ccggtcgata gttagaacgc tggcgctcat tcgtccgacg agatgaattt
     1681 cttgacttta tcgagctttc gcctcagtaa aatatgtacg gcctcgggat cctctagttt
     1741 tatagtaaaa tccctcgacc tagccaacaa gtccgcttca atatcggcca tacatttata
     1801 gatcgacgga tcgattccgt caacaatgcc cgcgaattta agtagcgaat cggacatcct
     1861 cttatagaat gcggcgttct ccaggtgaac gtactcggtc agctcgccgc gctcgtagcg
     1921 attctccata cgatcgacga gcaggcctat ctcgtctata aggtcgtcga gctcgaccct
     1981 gaacagcttg gcaaaatgcc tcgcttcgct actcatagcc agatcccccc ttacccgcga
     2041 tcgcgatgaa ctaagtatcg tggacggctt ctgctggcgc aagattacgg cgcgcttcct
     2101 cgtcagcggt atgaggataa aaaaagagac ccgcttgacg gcgggcctct tttttaagcg
     2161 ccagaaggga gtcgccttcc gctcgccgac atcctgtcgg cgtcgcttca ggccgcctca
     2221 gaacctctcg gcgcatcccg cgcctcgcgc tcgctagctc gcgacggttc ttcgattcga
     2281 ctcccttctg acgccgatat aaataaagag gccctcctta cggagggcct ctttatttaa
     2341 gcgccagaag ggagtcgaac cctcgtctcc agcttggaag gctggggtaa tagccgttat
     2401 acgacatccg ctctatcgag tcttgcatgc gcatttcgtt ctgcgcatga tctttatacc
     2461 aaaaaaacga tctc
//
LOCUS       DAOOLN010000162         2474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_52349_length_2474_cov_3.252170, whole genome shotgun sequence.
ACCESSION   DAOOLN010000162 DAOOLN010000000
VERSION     DAOOLN010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52349_length_2474_cov_3.252170"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..1395
                     /locus_tag="PLW80_02880"
     CDS             67..1395
                     /locus_tag="PLW80_02880"
                     /inference="COORDINATES: protein motif:HMM:NF002759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HPB65476.1"
                     /translation="MPALNTKALCDFIDASPTPYHAVDSAAAMLNAVGAVRLDERDAW
                     SLEVGRAYYVSRDGASLIAFRVGLTAPSESGFALAGAHADAPGLRARIEKSVAAKGMD
                     RVAVEAYGGPIHATWLDRPLSLAGRVAVRTPDGIHRRLVNFARPVAVIPNLAIHFNRE
                     MNKGVEYPMNQALLPLVSAAGPQPAGADGQAQGGSWVLRAVAAELGVEPDAIVSAELT
                     FVDAAASAAIGADSEIVSAPRIDNLEGCHAVLSAFVSAPTGGHGQIAALFDNEEIGSA
                     TPRGADSSFLRDIIERIVALTDDSGPEAAHRALARSFSVSVDGAQAWHPAYADRFDED
                     FAPVLNGGPAIKANANVRYATDAIGEAAFRACCEAAGVPCQRFRMRADLSPGTTIGPI
                     SSALLGVRTVDVGVPMLAMHSARETAGAYDHDHMIAALGRFYASGPSIAD"
     gene            complement(1484..2179)
                     /locus_tag="PLW80_02885"
     CDS             complement(1484..2179)
                     /locus_tag="PLW80_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65477.1"
                     /translation="MKRFVISFIALAMMAASVQAQSLADNSYYRKSVELEAAARAAFD
                     EGEYDEAAELAALAQENARLSDEYVAMILAMRKAKTAIESAQARYDWATGVRAELRFA
                     EDYAGATVELAAARAAFQAEAYAEALVHAYTVDAYLAGITDEEALPAYFVVRELSRRA
                     DCLWRIAGLPFVYNDPYKWPVLYEANKRSLPDPANPDLILPGMTLVIPAIENELRDGV
                     WIDGKEYPTFGTE"
     gene            complement(2184..>2474)
                     /locus_tag="PLW80_02890"
     CDS             complement(2184..>2474)
                     /locus_tag="PLW80_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65478.1"
                     /translation="VWDAAVAASAAGNHESAAPLFEQSEYMFEAVYQKAAEKRAAAEA
                     AIQAAKDKNERSASVAQRGDEQLGVEPAAQAESQATDEAASADGTSTAGEEE"
BASE COUNT          365 a          853 c          824 g          432 t
ORIGIN      
        1 gtcgctcgcc gagcggctgg cgactatacg gcatagctcg aaacacgaat aatctggagg
       61 taatccgtgc cggcactgaa caccaaggcc ttatgcgact tcatcgacgc gtcgccgacg
      121 ccgtatcacg ccgtcgactc ggcggcagcc atgctcaacg ccgtcggggc cgtccgcctc
      181 gacgagcgcg acgcgtggag cctcgaagtg gggcgagcct actacgtttc gcgggatggg
      241 gcgtcgctga tagccttcag ggttgggctc accgctccgt ccgagtccgg cttcgccctg
      301 gccggcgccc acgccgacgc cccgggcctg cgagcccgca tcgagaagtc ggtggccgcg
      361 aagggcatgg atcgagtcgc cgtcgaggcc tacggcggac cgatacacgc tacttggctc
      421 gataggccgc tgtcgctggc cggtcgcgtg gccgtccgga cgccggacgg catccaccgc
      481 cgcctggtga acttcgcgag gcctgtcgcc gtgattccca atctggccat ccacttcaac
      541 cgcgagatga acaagggcgt cgagtacccg atgaaccagg cgctgttacc gctcgtatcg
      601 gccgccggac ctcaaccggc cggggccgac ggccaggctc agggcggctc atgggtccta
      661 cgcgccgtgg cggccgagct cggcgtcgag ccggacgcca tagtctccgc cgagctgacc
      721 ttcgtcgacg cggccgcctc ggcggctatc ggcgccgact ccgagatcgt atcggcgccg
      781 cgtatcgata atctcgaggg ctgccacgcg gtgctgtccg ccttcgtatc cgccccgact
      841 ggcgggcacg gtcagatcgc ggccctcttc gataacgagg agataggctc ggccacgccg
      901 cgcggcgccg attcgtcgtt cctgcgggat attatcgagc gcatcgtggc gctgaccgac
      961 gattccggcc ccgaggccgc ccatagggcg ctcgccaggt cgttcagcgt gtcggtcgac
     1021 ggcgcgcagg cctggcatcc cgcctacgcc gatcgtttcg acgaggattt cgcgcccgta
     1081 ttgaacggcg gcccggccat aaaggccaac gccaacgtcc gctacgccac cgacgcgatc
     1141 ggcgaagccg ccttcagggc ttgctgcgag gccgcgggcg tcccgtgcca gcgtttcagg
     1201 atgcgcgccg acctatcgcc tgggacgacg ataggaccta tcagctcggc cctgctcggc
     1261 gtacgcaccg tcgacgtcgg cgtgccgatg ctcgcgatgc actcggcccg cgagacggcc
     1321 ggcgcctacg accacgacca tatgatagcc gccctcggcc gtttctacgc gtccgggcct
     1381 tcgatcgcgg attaggctat cccctcgact ggcccaaaaa aagggccgcc cgatcgggcg
     1441 gccctttcgt cgttcaggcg accgcgtcgc cttcgagcgt atcctactcg gttccgaagg
     1501 tcggatactc cttgccgtcg atccagacgc cgtcgcgcag ctcgttctct atcgccggga
     1561 tgaccagggt catacccggc aggatcaggt ccgggttggc gggatcgggc agagagcgct
     1621 tattggcctc gtagagtacg ggccacttgt acgggtcgtt gtagacgaag ggtaggccgg
     1681 ctatacgcca caggcagtcc gcgcgcctgg agagctcgcg taccacgaag taggcgggta
     1741 gggcttcctc gtcggtaatg cccgccaggt aggcgtctac ggtatacgca tggacgagag
     1801 cctcggcgta ggcctcggcc tggaaagcgg cgcgggccgc agccagctcg accgtggcgc
     1861 cggcgtagtc ctcggcgaag cgtagctcgg cgcggacgcc ggtcgcccag tcgtagcgcg
     1921 cctgggccga ttcgatcgcg gtcttggcct tgcgcatcgc caggatcatg gcgacgtact
     1981 cgtcggatag cctggcattc tcctgggcca gcgccgccag ctcggcggcc tcgtcgtact
     2041 cgccctcgtc gaaggcggcg cgggccgcag cctcgagctc gacgctcttg cggtagtagg
     2101 agttatcggc caggctctgg gcctggaccg aggccgccat catggcgagg gctatgaagc
     2161 tgatgacgaa tctcttcatg tcgttactcc tcctcgcctg ctgtagacgt gccatcggcg
     2221 ctcgcggcct catccgtcgc ctgactctcg gcttgcgccg cgggttcgac gccgagctgt
     2281 tcgtcgcccc gctgggctac ggaggcgctg cgctcgtttt tgtccttggc ggcctgaata
     2341 gccgcttcgg cggccgcacg cttctcggcc gctttctggt agacggcttc gaacatgtac
     2401 tcggactgct cgaacagggg ggccgccgac tcatggttgc cggccgccga cgccgcaacg
     2461 gcggcgtccc agac
//
LOCUS       DAOOLN010000163         2467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_52569_length_2467_cov_2.871891, whole genome shotgun sequence.
ACCESSION   DAOOLN010000163 DAOOLN010000000
VERSION     DAOOLN010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52569_length_2467_cov_2.871891"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PLW80_02895"
     CDS             complement(<1..691)
                     /locus_tag="PLW80_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB65479.1"
                     /translation="MMRDRMSAGPMSPEAIRAAVDGLDASLFEPAEFDASSGERGGYS
                     NYSYWRSTARGFIKNRVALVFLALLVTLLVFTFVQPLLPGQKSPTKIHIDPATGMQYR
                     NVEPGAEFWFGTNTIGQDLWSRIWSGTRTSLLIGLIVGAVEMVVGIAFGAVWGYVRRL
                     DGVITELYNVLNNIPTTIVLMLLSYILRPSFRTMVIAMCATGWLGLARFVRNQIVIIR
                     DREYNLASRCLG"
     gene            complement(688..1710)
                     /locus_tag="PLW80_02900"
     CDS             complement(688..1710)
                     /locus_tag="PLW80_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB65480.1"
                     /translation="MLGCRFDRFYKEECRRDGRASGGQGQMLKYTVRRLLQSLVTLLI
                     VVSAVFLLMRLLPVEGYFGDGYDKLSESQREAVLQKMGLLDPWYIQLGSFYAGLLRGD
                     LGVSTIYRQDVPVLEVVAPRIPYSMRFGLVAIAIALVAGLGMGVSMAKHKGRALDKLW
                     TGYVVFVSAVPLAVYLLFIQLYVTELFGLPLLFDKYRPSSWILPALSMSLGSIAGYAM
                     WMRRYMVDELNKDYIRLAMAKGLPRSAIMVRHVMRNAYVPMAQYLPASILMTISGSIY
                     IESLYSIPGMGGLLVDVIQRQDNPMVQGLVLIFSSIGIVGLFLGDLLMALFDPRIKLA
                     KSEASR"
     gene            1870..>2467
                     /locus_tag="PLW80_02905"
     CDS             1870..>2467
                     /locus_tag="PLW80_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB65481.1"
                     /translation="MKKKQALAIAVFALFTIGQAWSQRKSADYYEMYAGEVTTLNYLI
                     SGSENEHIMFANVIDNLIEYDRYGVVQPGLAESWTTSPDGLVWTFKLRKNLRWYTWQG
                     KEYGDVTAQDWVDAAKYILTKANASRIVEPLSSVVKNADAYYEGKLSDFAQVGVRALD
                     KGTLEYTLEKPVPYFLSMLSYVTYLPVSGKFLAEAGAAF"
BASE COUNT          502 a          764 c          788 g          413 t
ORIGIN      
        1 tgccgagaca gcgcgacgcg aggttgtact cccggtctct gataatgacg atctggttgc
       61 gcacgaacct ggcgagcccc agccagccgg tcgcgcacat cgctatgacc atggtcctga
      121 agctcggccg gaggatatac gacagcagca tcagcacgat ggtcgtcggt atgttgttga
      181 gcacgttgta gagctcggtg atcacgccgt cgagcctgcg cacgtagccc cagacggccc
      241 cgaaggcgat gccgactacc atctcgacgg cgccgacgat caggccgatc aggagcgagg
      301 tgcgcgtgcc gctccagatc ctcgaccaga ggtcctgccc tatcgtgttc gtgccgaacc
      361 agaactcggc gcccggctcg acattcctgt actgcatgcc ggtggccggg tctatatgaa
      421 tcttggtcgg gctcttctgc ccgggcaaga gcggctgcac gaaggtgaag acgagcagcg
      481 tgacgagcag cgcgaggaag accaaggcca ccctgttctt gatgaaaccg cgcgccgtcg
      541 agcgccagta cgagtagttg gagtagccgc cgcgctcgcc cgagctcgcg tcgaactcgg
      601 cgggctcgaa cagggaggcg tcgaggccgt ccacggcagc gcggatcgcc tcggggctca
      661 tcggcccggc gctcatcctg tcccgcatca tcgcgaggcc tcgctcttgg ccagcttgat
      721 gcgcgggtcg aagagcgcca tcaggaggtc cccgaggaac agcccgacga tgcctatcga
      781 cgagaagatg agcacaaggc cctggaccat cgggttgtcc tggcgctgga tcacatcgac
      841 gaggagccca cccatgcccg gtatcgagta cagcgactcg atatagatgg agccggatat
      901 ggtcatcagg atcgacgcgg gcaggtactg ggccatcggc acgtaggcgt tgcgcatcac
      961 gtgcctgacc atgatcgcgg agcgcggcag ccccttggcc atcgccaggc ggatgtagtc
     1021 cttgttgagc tcgtcgacca tgtaacgtcg catccacatg gcgtagccgg cgatgctgcc
     1081 gagcgacatc gacaaggcgg gcagtatcca gcttgacggc cggtacttgt cgaacagcaa
     1141 cggcaggccg aagagctcgg ttacgtacag ctggatgaac agcaggtaca cggcgagcgg
     1201 cacggcgctg acgaacacga cgtagccggt ccagagcttg tccagcgcgc ggcccttgtg
     1261 cttggccatc gacacgccca tccccaggcc ggcgacgagg gcgatcgcta tggcgacgag
     1321 gccgaagcgc atggagtacg gtatcctcgg cgcgacgacc tcgaggaccg gcacgtcctg
     1381 acggtatatc gtcgacacgc cgagatcccc gcgcaggagg ccggcgtaga acgagccgag
     1441 ctggatgtac cagggatcca ggagccccat cttctggagc acggcctcgc gctggctctc
     1501 gctgagctta tcgtagccgt cgccgaagta gccctcgacg ggcaggaggc gcatcagcag
     1561 gaacaccgcc gagacgacga tgagcagggt gacgagcgac tgcaggagcc gccggacggt
     1621 atacttgagc atctgaccct gtccgccact ggcccggcca tcgcggcggc actcctcctt
     1681 gtagaagcgg tcgaagcgac atcctaacac ggtttggccg atgggaagcc gggaaatttc
     1741 atggtgggat catggcatcg ttcctacggg aggaaacgtt tcgtttgacg agcgttacta
     1801 tcgatggaat acttctagcg acgacggtgt taccgcccta cgatttctga tcggataagg
     1861 aaacgacgca tgaagaagaa acaggctctg gcgatagccg tattcgcgct gttcacgatc
     1921 ggccaggcat ggtcccagcg gaagtccgcc gattattacg agatgtacgc gggcgaggta
     1981 acgaccctca actacctgat ctcgggctcg gaaaacgagc atatcatgtt cgccaacgtc
     2041 atcgacaacc tgatcgagta tgaccggtac ggcgtcgtgc agccgggcct cgccgaatcg
     2101 tggacgacct cgcccgacgg actcgtctgg accttcaagc tcaggaagaa cctacgctgg
     2161 tacacctggc agggcaagga atacggcgac gtgacggccc aggactgggt cgacgcggcc
     2221 aagtacatcc tgaccaaggc gaacgcctcg cgcatcgtcg agccgctatc ctcggtcgtc
     2281 aagaacgccg acgcctacta cgagggcaag ctatcggact tcgcgcaggt cggcgtacgg
     2341 gcgctcgaca agggcacgct cgagtatacc ctcgagaagc cggtaccgta cttcctgtcg
     2401 atgctcagct acgtcaccta cctaccggtc agcggcaagt tcctggcaga ggctggcgcc
     2461 gccttcg
//
LOCUS       DAOOLN010000164         2467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_52570_length_2467_cov_2.809287, whole genome shotgun sequence.
ACCESSION   DAOOLN010000164 DAOOLN010000000
VERSION     DAOOLN010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52570_length_2467_cov_2.809287"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PLW80_02910"
     CDS             complement(<1..216)
                     /locus_tag="PLW80_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor N-terminal transmembrane
                     domain-containing protein"
                     /protein_id="HPB65482.1"
                     /translation="MRRPFLGRLSVRLLAFNLLLVFLPLASLLYLDTYEKQLLEMQEA
                     SMIQQGRVFASAMAGVDLAAESTTILAR"
     gene            complement(213..920)
                     /locus_tag="PLW80_02915"
     CDS             complement(213..920)
                     /locus_tag="PLW80_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB65483.1"
                     /translation="MNRIAVVDDEKNIRETIRLALEREGYAVEEYANGEEAWAAFRRR
                     WPDLAILDIMMPRMDGMELCRRVRAESTMPIIFLSSRDDELDRVLGLESGGDDYVCKP
                     FGMRELTARVRAALRRASPEPGTGVAAAYGLRAGRLELDDERYLVRWDGREAALTVTE
                     FRMLWNLAELPGAVRTREQLMRAAFPEDAWPNDKAADSHIKRIRAKLSAVDPGFDGIE
                     TVYGLGYRLGGEGGRAR"
     gene            1067..2443
                     /gene="creD"
                     /locus_tag="PLW80_02920"
     CDS             1067..2443
                     /gene="creD"
                     /locus_tag="PLW80_02920"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HPB65484.1"
                     /translation="MDGIRRITGSMGFKAALIAFLVLVMLIPMGLVRQVVGERADRAE
                     EAADEIVESWGGELRLAGPLLRIPCVRADEAIRRGDGGAETVERRTTAFDLWVGPTHL
                     GLKAEVASETRSRGVFGVPVFSGPVAFEGRFDAAEALGSLRDNEAAMLGEAELVMAIE
                     SQKGLRGIDGATWGGKPLVFRPGDAGLGLLSGGVKAAAPLESGAGADFALTASVQGGR
                     RLRLLPLGSSTSATVSSDWPAPSFQGEYLPASRGLGDDGFSATWRVSDLSRGIPSYWR
                     SGELPRGAMDRFYFGVDFFEALDRYALSERAAKHALLFVVVPFMTLFLLELFARRAVH
                     PIQYLLAGVANLVFYLLLLSLSEQVPFGAAYLIAASATGGLTFLYSWSMFKDLRKAWC
                     MAPVMGLSYLYLYAALRSEDSALLFGAIGAFAVTALVMFVTRDVDWYGYMKKAPGLAA
                     RGDDGASE"
BASE COUNT          336 a          863 c          862 g          406 t
ORIGIN      
        1 ccgggccagg atcgtcgtcg actcggccgc gaggtcgacg cccgccatcg ccgaggcgaa
       61 cacgcggccc tgctggatca tcgaggcctc ctgcatctcc aggagctgct tctcgtaggt
      121 gtccagatac aggaggctgg cgagcggcag gaagacgagc agcaggttga aggccaggag
      181 gcggaccgag agccgcccga ggaacggccg cctcatcgcg cccggccgcc ttcgccgccc
      241 aggcgatagc cgagcccgta gaccgtctcg atgccgtcga agcccgggtc gacggcggac
      301 agcttggctc ggatccgctt gatatggctg tcggccgcct tgtcgttcgg ccaggcgtcc
      361 tccgggaagg cggcccgcat cagctgctcg cgcgtgcgca ccgctcccgg tagctcggcc
      421 aggttccaga gcatgcggaa ctcggtcacg gtgagcgccg cctcgcggcc gtcccagcgc
      481 accaggtagc gctcgtcgtc gagttccagc ctgcccgccc gcaggccgta cgcggcggcg
      541 accccggttc cgggctccgg agaggctcgg cgcagggcgg cgcgcaccct ggcggtcagc
      601 tcccgcatcc cgaagggctt gcacacgtag tcgtcgccgc ccgactcgag gccgagcacc
      661 cggtccagct cgtcgtcgcg cgacgacagg aagatgatgg gcatcgtcga ctcggcccgc
      721 acgcgccggc acagctccat gccgtccatc ctgggcatca tgatgtccag gatggccagg
      781 tcgggccagc ggcgcctgaa ggccgcccag gcctcctcgc cgttggcgta ctcctcgacg
      841 gcgtagccct cgcgctcgag ggccagccgt atcgtctcgc gtatgttctt ctcgtcgtcg
      901 acgactgcta tgcggttcat cagccgcgag gatagcacgg cccgtcggcc gcgtgaatcc
      961 ccggacggcc gggaaccggg cggaagccgg aatgctgcca ccttccggcc accctgcggc
     1021 ccgattggcg gggtagcctc tcgcaagtcc cccggaagga gccactatgg acggtatacg
     1081 aagaatcacc ggcagcatgg gtttcaaggc ggccctcatc gcctttttgg tactggtcat
     1141 gctgatcccg atgggcctgg tcaggcaggt cgtcggcgag cgagccgatc gcgccgagga
     1201 ggccgcggac gagatagtcg agtcctgggg cggcgagctc aggctggccg gccccctcct
     1261 caggataccc tgcgtacgcg ccgacgaggc gatccggcgc ggagacggcg gcgcggagac
     1321 cgtcgagcgc cggactacgg ccttcgacct ctgggtcggc ccgacccacc tcggcctgaa
     1381 ggccgaggtc gcgagcgaga cgaggagccg aggcgtcttc ggcgtgccgg tgttctccgg
     1441 cccggtagcc ttcgagggcc gcttcgacgc ggccgaggcg ctcggtagcc tgcgcgacaa
     1501 cgaggccgcc atgctcggcg aggccgagct ggtcatggcc atcgaaagcc agaagggcct
     1561 gcgcggcatc gacggcgcca cgtggggcgg gaagcctctc gtcttcaggc ccggcgacgc
     1621 cggtctcggc ctcctgtcgg gcggcgtcaa ggcggcggct ccgctcgagt ccggcgccgg
     1681 agcggacttc gcgctgacgg cctcggtgca aggcggccgc cggctcaggc tactgccgct
     1741 cggctcgtcg acgtccgcga ctgtatcgtc cgattggccc gccccgtcgt tccagggcga
     1801 gtacctaccg gccagccgtg gtctcggcga cgacggcttc tccgccacct ggcgagtcag
     1861 cgacctgagc cgcggcatcc cgtcgtactg gcgctcggga gagctcccgc gtggcgcgat
     1921 ggaccgcttc tacttcggcg tcgacttctt cgaggcgctc gaccgctacg cgctttccga
     1981 gcgggccgcg aagcacgccc tgttgttcgt cgtcgtgccg ttcatgacgc tgttcctgct
     2041 cgagctgttc gcgcgtcggg ccgtgcatcc gatccagtac ctgctggccg gcgtggcgaa
     2101 cctggtcttc tacctcctgc tcctgtcgct gtcggagcag gtcccgttcg gggcggccta
     2161 cctgatcgcg gcctcggcga cgggtggcct gaccttccta tactcgtggt cgatgttcaa
     2221 ggatctccgc aaggcctggt gcatggctcc ggtgatgggg ctttcgtacc tgtacctgta
     2281 cgccgccctg cgctcggagg acagcgctct gctcttcggc gcgataggcg cgttcgcggt
     2341 gaccgcgctc gtcatgttcg tgacgaggga cgtggactgg tatggctata tgaagaaagc
     2401 cccgggcctt gcggcccggg gcgacgatgg agcctcggag taaggctcgg gctacgcctc
     2461 gccctcg
//
LOCUS       DAOOLN010000165         2456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_52946_length_2456_cov_2.487713, whole genome shotgun sequence.
ACCESSION   DAOOLN010000165 DAOOLN010000000
VERSION     DAOOLN010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52946_length_2456_cov_2.487713"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="PLW80_02925"
     CDS             complement(<1..1248)
                     /locus_tag="PLW80_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPB65485.1"
                     /translation="MASGKSVSKVLKILIVSAVVVAIALGVAFGVAIASVRNIMSVEN
                     FSTFDPPLPSRLLASDGRLITEFASSEKRELVSIKDMPQHLIDALVTREDQSFWTHPG
                     FTLKGYFRAFVGIVTGRNMGGGSTLTLQLAGTLYADRRDISFRRKFVELWWALQLERR
                     FSKQEILEMYMNRMIMGPGVYGVEAASKYFFGHSAKDVSIAESAILVIQLSSPTRYNP
                     LRNPNVARDRSREVLNQMIELGYVTKDAADESFALYWDNYDYTRVASGAFYARDDKAP
                     WFSEYVRRDLEKMLYGSIDLYKDGLTIHTTLDLDYQAVADKYMSRYIAQVNRDYASTS
                     SRRLKEAERIYAPMAELLGLAFNLEGMFFEETKVRGKAMEYYRKSVNPTVDAMALMFG
                     LEPLKLATNASYGAAKTEMEKNTV"
     gene            1555..>2456
                     /gene="gap"
                     /locus_tag="PLW80_02930"
     CDS             1555..>2456
                     /gene="gap"
                     /locus_tag="PLW80_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPB65486.1"
                     /translation="MKVAINGFGRIGRLVFQSIVDQGLLGKDKIDVVAVVDVATDAKY
                     FAYQLKYDSVHGRMNAKISSKKSDPSLEEDDVLVVNGHEVKCVMAEKELKNLPWAKLG
                     VEYVIESTGIFSNEKAYDHLEAGAKKVIISAPAKSKDESKKIKTFVVGVNENDYDASK
                     HHVISNASCTTNCLAPVVHVLLKEGIGIETGLMTTIHSYTATQKTVDGVSKKDWRGGR
                     AAAINIIPSTTGAAKAVGEVLPSTKGKLTGMSFRVPTSDVSVVDLTFRSEKDISIEEI
                     DALLKKASESYLKGILGYGEEELVS"
BASE COUNT          516 a          717 c          747 g          476 t
ORIGIN      
        1 gacggtattc ttctccatct cggtcttggc ggctccgtag ctggcgttgg tcgccagttt
       61 gagcggctcg aggccgaaca tgagcgccat cgcgtccacg gtggggttga cgctcttccg
      121 gtagtattcc atggccttgc ctcggacctt ggtctcctcg aagaacatcc cctcgaggtt
      181 gaaggcgagg ccgagcagct cggccatcgg cgcatagatc cgctcggctt ccttgaggcg
      241 gcgcgagctg gtcgaggcat agtcgcggtt gacctgggct atatagcggc tcatgtactt
      301 gtcggccacc gcctgatagt cgaggtcgag cgtcgtgtgg atggttagac cgtccttgta
      361 gaggtcgatc gagccgtaca gcatcttctc gaggtcgcgt cggacgtact cgctgaacca
      421 gggggcctta tcgtcgcggg cgtagaacgc gcccgaggcg acgcgggtat agtcgtagtt
      481 gtcccagtac agcgcgaagg actcgtctgc ggcgtccttc gtcacgtagc cgagctctat
      541 catctggttg agcacctcgc gcgagcgatc cctcgcgacg ttggggttcc tcagtggatt
      601 gtaacgggta gggctcgaca gctggatcac gaggatggcc gactcggcga tgctgacatc
      661 cttggcggaa tgcccgaaga agtacttgct ggccgcctcg acgccgtaga ctcccggccc
      721 catgatcatg cggttcatgt acatctcgag gatttcctgc ttcgagaagc gccgctcgag
      781 ctggagggcc caccacagct cgacgaattt gcgcctgaag cttatatccc gacgatcggc
      841 gtacagggtg ccggcgagct gcagggtcag cgtggagccg ccgcccatat tccgtccggt
      901 cacgatgcct acgaaggccc tgaagtagcc cttgagcgta aagccggggt gggtccagaa
      961 cgactgatcc tcgcgggtca cgagggcgtc gatcaggtgc tgcggcatat ccttgatcga
     1021 gacgagctct cgcttctcgc tcgaggcgaa ctcggtaatg agccgcccgt cgctagcgag
     1081 tagcctggac ggcaggggcg ggtcgaaggt ggagaaattc tcgaccgaca tgatattgcg
     1141 taccgacgct atagcgaccc cgaacgcgac gccgagcgct atggcgacga cgacggcgga
     1201 gacgatcaat atcttcagta ctttgctgac ggacttcccg gatgccatat cgccgaaagc
     1261 ctacgcgaaa gctcgccggt aggtcaagct ggatataaaa aaggccggtc ccgaagaccg
     1321 gcccgccctg acaggctgtc tgcctccgcc gcgacgaatt gggagggggg ccgcgacggc
     1381 gcccgacatt ctatatatcg gaaccggaac gcgtttttta aatataaata attgcatagg
     1441 gaacgcatga ttgcgtatat ttatccacgc attgacgaac gattctactt accgatatca
     1501 ttcgagaaaa taagatactg ttttatcgat tcttcttatg cataggaggc ctcaatgaag
     1561 gttgcaatca acggtttcgg ccggatcggg cggctggtct tccagtccat cgtcgaccag
     1621 ggactgctcg gcaaggacaa gatcgacgtg gtggccgtag tggacgtcgc cacggacgcc
     1681 aagtacttcg cctaccagct caagtacgat tcagttcacg gccgcatgaa cgccaagatc
     1741 tcctccaaga aaagcgaccc gtcgctcgag gaggacgacg tgctcgtggt caacggccac
     1801 gaggtcaagt gcgtcatggc cgagaaggag ctcaagaacc tgccctgggc caagctgggc
     1861 gtcgagtacg tcatcgagtc caccggcatc ttctcgaacg agaaggcgta cgatcacctc
     1921 gaggcgggag ccaagaaggt cattatcagc gctccggcca agagcaagga cgaatccaag
     1981 aagatcaaga ccttcgtcgt cggcgtcaac gagaacgatt acgacgcctc caagcatcac
     2041 gtcatctcga acgcgtcctg cacgacgaac tgcctcgcac cggtcgtcca tgtactgctc
     2101 aaggaaggca ttggcatcga gaccggcctg atgacgacca tccatagcta caccgccacc
     2161 cagaaaaccg tcgacggtgt gtccaagaag gactggcgcg gcggtcgagc cgccgccatc
     2221 aatatcatcc cgtctacgac cggtgccgcc aaggcagtcg gagaagtgct cccgagcact
     2281 aagggcaagc tgaccggcat gtcgttccgc gtgccgacgt ccgacgtttc ggtcgtcgac
     2341 ctgaccttcc gcagcgagaa ggacatctcg atcgaggaga tcgacgctct gctcaagaag
     2401 gccagcgaga gctacctcaa gggcatcctc ggctatggcg aggaggagct ggtatc
//
LOCUS       DAOOLN010000166         2447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_53227_length_2447_cov_2.938545, whole genome shotgun sequence.
ACCESSION   DAOOLN010000166 DAOOLN010000000
VERSION     DAOOLN010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53227_length_2447_cov_2.938545"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>698)
                     /locus_tag="PLW80_02935"
     CDS             complement(<1..>698)
                     /locus_tag="PLW80_02935"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HPB65487.1"
                     /translation="DSDGYAGGGANGQAEDFGGKLISRLVVNVSLPAYMIANLMGGYD
                     RARLLSMLPGLPIPFLSMAAAFASSAAVAALFKIRPERRGTFSSMASLSNTIFIGLPV
                     NMMIFGEESLPYVLIYYIANTVMFWTLGVYGISGDGARLAGNPAPRFLSPTGLRRLLS
                     PPLLGLMVAIVLIMLGVALPKPLLDTARTIGSMTTPLSMLFIGIVIAKVDWRSIRLER
                     DLVLVALCRFVVSPA"
     assembly_gap    699..798
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<799..1005)
                     /locus_tag="PLW80_02940"
     CDS             complement(<799..1005)
                     /locus_tag="PLW80_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65488.1"
                     /translation="MLNALGSVFSVLLMIALGFVLARRSWFSASGYAGGGANGQAVRE
                     RASKDSDGYAGGGANGQAVRERASK"
     gene            complement(1049..2263)
                     /locus_tag="PLW80_02945"
     CDS             complement(1049..2263)
                     /locus_tag="PLW80_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPB65489.1"
                     /translation="MKSTKGEQVWLAAGAASAAMAAFLVWAFAQPGTLGMYRWGYRAP
                     VLGLIALGALPIVAAAAARALSRTKGAAPRIATGIVFAASAVTLLTAISVSAYIFTNA
                     YGRAASPDRPTTIAPSDGIPRRTGADGEPILRVAVSSDGHYGRDASNAEARSAILRLS
                     EVEHRSGGLDAFINLGDIVEMGMEADDWREAIESIRRDAPTLPFLALLGNHDALIGGY
                     ARWQAAFGPKGGSSAPTGLSPSSWRIDAGRVHFIALDLLWGPEGFGAKEKAWLVAQLT
                     AIPPSDYTVVLSHCFFYSSGYVDEETGMAWYDHADMLREVAPLIAGKADLVISGHNHY
                     MEWIEADGTAWAVVGALGGKPDPVPSYVSPGSVWRNQGVFGRLVLELTPDGLACEFQD
                     QDGTPLFNRVLR"
BASE COUNT          423 a          736 c          795 g          393 t
ORIGIN      
        1 gccggcgaga cgacgaagcg gcagagggcc acgagtacca ggtcgcgttc gaggcgtatc
       61 gagcgccagt cgacctttgc gatgacgatg ccgatgaaca gcatcgaaag gggcgtggtc
      121 atcgagccta tggtcctggc cgtgtcgagg agtggcttgg gtagggcgac gccgagcatg
      181 atgaggacga tggcaaccat gaggccgagg agcggcggag acagtagcct gcggagcccc
      241 gtcggagata agaaccgcgg ggctgggttc ccggccaggc gggccccgtc tcctgagatg
      301 ccatagacgc cgagggtcca gaacatgacg gtattggcga tatagtagat gaggacgtag
      361 ggcaggctct cctcgccaaa gatcatcatg ttaacgggta ggccgatgaa gatggtgttc
      421 gatagcgacg ccatcgacga gaaggtgccg cggcgttccg gtcgtatctt gaatagggcg
      481 gctacggcgg cactcgacgc gaaggcggcc gccatcgaca ggaaggggat cggcaagccc
      541 ggtagcatcg acaggagcct ggctctgtcg tagccgccca tcaggttcgc tatcatgtag
      601 gcgggtaggg acacgttgac gacgagccgc gatatcagct tgccgccgaa atcctcggct
      661 tggccgttcg cgccgcctcc ggcgtagccg tcactatcnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnct tggatgcacg ttcgcgaacg gcttggccgt tcgcgccgcc
      841 tccggcgtag ccgtcactat ccttggatgc acgttcgcga acggcttggc cgttcgcgcc
      901 gcctccggcg tagccggagg cgctgaacca agaccgcctg gccagcacga aaccgagggc
      961 tatcatcaag aggaccgaga acacgctacc gagggcgttg agcatggcgc catcctatcg
     1021 ggaagggcgt tccatgctca agcccgggct atcgcaggac tcggttgaac agcggcgtgc
     1081 cgtcctgatc ctggaactcg caggcgaggc cgtcgggcgt cagttcgagc acgagccttc
     1141 cgaagacgcc ctggttccgc cagaccgagc ctggcgagac gtagctgggc acggggtcgg
     1201 gcttgccgcc aagcgcgccg actacggccc acgcggtgcc gtcggcttca atccattcca
     1261 tatagtgatt gtgcccggag atgacgaggt cggcctttcc ggctatgagc ggcgctacct
     1321 cgcgcagcat gtcggcgtga tcgtaccagg ccattcccgt ctcctcatcg acatagccgg
     1381 agctatagaa gaagcagtgc gaaagtacga ccgtgtaatc cgacggcggg atagccgtca
     1441 gctgggcgac gagccaggcc ttctccttgg cgccgaagcc ttcaggaccc cagagcaggt
     1501 cgagggctat gaagtggacg cgtccggcgt cgattcgcca tgacgatggg ctcaggccgg
     1561 taggcgccga agagccgcct ttcggcccga atgcggcctg ccagcgggcg tagccgccta
     1621 tgagcgcgtc atggttgccg agcagggcca ggaagggcag ggtcggggcg tcgcgccgta
     1681 tcgactcgat ggcctcccgc caatcgtcag cctccatgcc catctcgacg atgtcgccga
     1741 ggttgataaa ggcgtcgagc ccgcccgagc gatgctccac ctcggatagc cgtagaatag
     1801 cgctcctggc ctcggcgttc gacgcgtcgc ggccgtagtg cccatccgaa gagacggcta
     1861 ccctgaggat cggttcgccg tcggcgcccg tacgtctagg gataccgtcg ctcggggcta
     1921 tggtcgtcgg gcgatcaggc gatgccgccc ggccgtacgc gttcgtgaaa atgtacgcgg
     1981 atacggagat tgccgtcagg agcgtgacgg ccgacgcggc gaagacgatg ccggtcgcta
     2041 tcctcggcgc cgcgcccttg gttcggctca gggccctggc ggccgccgcc gcgacgatag
     2101 gtagcgcgcc gagcgctatc aggccgagca ccggcgctcg gtacccccat cggtacatgc
     2161 cgagcgtgcc gggctgggcg aatgcccata ccaggaacgc agccatggcc gcgctagccg
     2221 caccggcggc tagccagacc tgttcgcctt tcgtcgattt catcggagcc tccgcgctta
     2281 gcctacgacg cgctcgccgg acggtcaagc gatccgtagt gtcacgaagc ctattggttt
     2341 ttaaacaagg cggccgcccc attcggagcg gccgcggact cgttcagagg gccgaagccc
     2401 tcctggcaaa gtcgatcatc gatcacctcc tttctccggc gctcgcg
//
LOCUS       DAOOLN010000167         2446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_53260_length_2446_cov_2.815558, whole genome shotgun sequence.
ACCESSION   DAOOLN010000167 DAOOLN010000000
VERSION     DAOOLN010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53260_length_2446_cov_2.815558"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1698
                     /gene="ftcD"
                     /locus_tag="PLW80_02950"
     CDS             <1..1698
                     /gene="ftcD"
                     /locus_tag="PLW80_02950"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HPB65490.1"
                     /translation="AAKKEISQMAERRIVECVPNFSEGRDKGVIDAIAKSIEGVQGVS
                     LLDVDPGADTNRTVYTFVGESDAVLEAAVRSAATARSLIDMAKHSGAHPRMGALDVCP
                     FVPVSGVTMDECVALAKRAAERMAAELDVPVYLYEYAASRPERRSLADIRSGEYEALA
                     DKLARPEWKPDYGPARFVPGWGATVVGARDFLIAYNVNLNTRDRKLANEVALNVREGG
                     RAARDAAGNIVKDGKGEKVMVPGRLKNVRAIGWYIDSYRRAQVSINLTNYKATPLHVV
                     YETVKEEAEKLGLSVCGSELVGLVPLEPLVEAGRYYLRRMGRSEGAPEPELVELAKTT
                     LGLDSVAAFDAERKIIDYAARPAAPLMAMTAAAFVDEVSSDSPAPGGGSVSALAGSLS
                     AALSAMVANLTVGKKGYEPVWEPMSALAVRAQAVKQALTRAVDDDTSAFNAVMDAMKL
                     PKGTAEEKAAREAALQAGYKSAVEVPLATAEACVAALELAIEATAGNRNSSSDAGVAA
                     LLARAGAHGAALNVLINLGSIADAAYVDGMKARVAALRTKADELCARAVAAVEATFA"
     gene            complement(1865..2155)
                     /locus_tag="PLW80_02955"
     CDS             complement(1865..2155)
                     /locus_tag="PLW80_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPB65491.1"
                     /translation="MADTILDDPIMRMIDGGAKVIDVRSPEEYDDEHYPNAINIPLNL
                     IQAKAAELGDKSAPLVLYCASGSRSAFAARMLSMAGFKNVVNAGGLYDMPGY"
     gene            complement(2195..>2446)
                     /locus_tag="PLW80_02960"
     CDS             complement(2195..>2446)
                     /locus_tag="PLW80_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65492.1"
                     /translation="TNGDRRKAPVPSAVQIPLDELDWRLEELPAGRLLLLSRAGYESH
                     IALRKLLGLGRTDAQNISGGELSLELVPGFVRVLESIER"
BASE COUNT          421 a          800 c          824 g          401 t
ORIGIN      
        1 gcggcgaaaa aggagatttc tcagatggcc gaacgcagga tagtcgaatg cgtccccaat
       61 ttcagcgagg ggcgcgataa gggcgtcatc gacgccatag ccaagtcgat cgagggcgtc
      121 cagggcgtgt ccctcctcga cgtcgatccc ggcgccgaca ccaatcgcac cgtgtacacc
      181 tttgtaggcg agagcgacgc cgtactcgag gcggctgtcc gttccgccgc gacggcccgc
      241 tcgcttatcg acatggccaa gcactccggc gcccatccgc gcatgggcgc cctcgacgtc
      301 tgccctttcg tgccggtctc cggcgtgacg atggacgaat gcgtcgccct ggccaagcgg
      361 gcggccgaga ggatggccgc cgaactcgat gtgccggtct acctctacga gtacgccgcc
      421 agccgcccgg agcggcgtag cttggccgac atacgctccg gagagtacga ggccctggcc
      481 gacaagctcg cgaggcccga gtggaagccg gactacggcc cggcccgttt cgtacccggc
      541 tggggcgcca cggtcgtagg cgcccgcgac ttcctgatag cgtacaacgt caacctgaac
      601 acgcgcgatc ggaagctggc caacgaggta gcgctcaacg tgcgcgaggg cggcagggcc
      661 gcccgcgacg ccgccggcaa catcgtcaag gacggcaagg gcgagaaggt catggtgccc
      721 ggccgcctca agaacgtccg ggccatcggc tggtacatcg actcgtaccg cagggcccag
      781 gtgtcgatca acctgaccaa ctacaaggcc acgccgctcc acgtcgtcta cgagacggtc
      841 aaggaggagg cggagaagct gggcctgtcg gtctgcggct ccgagctcgt cggcctcgtg
      901 ccgctcgagc ccctcgtcga ggcgggccgc tactacctgc gccgtatggg caggagcgag
      961 ggcgcgcccg agccggaact ggtcgagctg gccaagacga cgctcggcct cgactcggtg
     1021 gcggccttcg acgccgagcg caagatcatc gactacgccg cgagacccgc ggccccgctc
     1081 atggcgatga cggccgcggc cttcgtcgac gaggtgtcga gcgactcccc cgcgcccggt
     1141 ggcggctcgg tctcggcgct cgccggctcc ctgtcggccg ccctgtcggc tatggtcgcc
     1201 aatctaaccg tcggcaagaa gggctacgag ccggtctggg agccgatgtc cgccttggcg
     1261 gtcagggctc aggctgtcaa acaggcgctc acccgggccg tggacgatga cacgagcgcc
     1321 ttcaacgccg tgatggacgc gatgaagctg ccgaagggca cggccgagga gaaggcagcg
     1381 cgagaggcgg ccctgcaggc cggctacaaa tcggcggtcg aggtgccgct ggccaccgcc
     1441 gaggcctgtg tagccgcgct cgagctggct atcgaggcta cggccggcaa caggaactcg
     1501 tcatccgacg cgggcgtcgc cgcgctcctc gccagggccg gggcccacgg cgccgcgctc
     1561 aacgtgctga tcaacctcgg ctcgatagcc gatgcggcct atgtcgatgg catgaaggcc
     1621 cgcgtggccg cgctcaggac caaggccgac gagctatgcg cccgggccgt agccgccgtc
     1681 gaagccacct tcgcatagtg tggatactct ataaggcgat gaagagaata cgaggatatg
     1741 aggtaggaaa gagaatctcc gagtaaaggt agcaatacga agaggcgacc aggccttggg
     1801 cctggtcgcc tcttgcttta cctctggtgc tcgtattccc tttgtgctgt attctacgtc
     1861 ggactcaata gccgggcatg tcgtagaggc cgccggcgtt gaccacgttc ttgaagccgg
     1921 ccatcgacag catcctggca gcgaaggcgc tcctcgaccc ggacgcgcag tacagcacga
     1981 gcggcgcgga tttgtcgccc agctctgccg ccttggcctg gatcaggttg agcggaatgt
     2041 tgatcgcgtt cgggtagtgc tcgtcgtcgt actcctcggg gctccgcacg tctatcacct
     2101 tcgcgccgcc gtcgatcatc ctcatgatgg gatcgtctag tatcgtgtct gccatagttc
     2161 acctccaaaa tgtgtacgcc tcgaaatcgg gcgcttatcg ttcgatcgac tcgagcaccc
     2221 ggacgaagcc ggggacgagt tccaggctca gctcgccgcc cgagatgttc tgagcgtcgg
     2281 tcctgcctag gccgaggagt ttgcgtaggg cgatgtggct ctcgtagccg gccctggaca
     2341 ggaggaggag tctaccggcg ggcagctcct cgagcctcca atcgagttcg tcgaggggga
     2401 tttggactgc cgacgggacc ggcgccttgc ggcggtcgcc gttggt
//
LOCUS       DAOOLN010000168         2439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_53471_length_2439_cov_2.911913, whole genome shotgun sequence.
ACCESSION   DAOOLN010000168 DAOOLN010000000
VERSION     DAOOLN010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53471_length_2439_cov_2.911913"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PLW80_02965"
     CDS             complement(<1..559)
                     /locus_tag="PLW80_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB65493.1"
                     /translation="MAHTGNEAALTGTGAYDCVVVGAGLGGLCAAFELSRAGAKTLLL
                     EGHNLPGGFATSFVRGRFEFEPSLHELPDMRSMSEATGVVRYLLDDAGLEVGFASVPE
                     AYRLILTDDGVDLRMPFGIEACIAEVERAVPGSRPSVSAYFELCREVQDGFGYLNAHA
                     RSPNVLKLLRDHGDFVRTASATVARA"
     gene            994..1413
                     /locus_tag="PLW80_02970"
     CDS             994..1413
                     /locus_tag="PLW80_02970"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPB65494.1"
                     /translation="MVAYLDSSVMLRYILLGETALRHVQAFPRLASSELLEIECRRVL
                     LRCRIEGELNDESLVEATSRLDEALSGVDLLELDAAVKKRAMEAFPIHVKTLDALHLA
                     SALQLLVEEPADGIVVFSHDRTFNLCARALGFNAALL"
     gene            1496..1984
                     /locus_tag="PLW80_02975"
     CDS             1496..1984
                     /locus_tag="PLW80_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HPB65495.1"
                     /translation="MSSCQFASLDAIVPAPAFIREAAYAEAERYLGMEYEWGGQDFPR
                     GIDCSGLVVNVYHTVTAGTEYAPPFDDATAAMLSERYTVDIDVPARGDLVFMGDGAVS
                     HVAILDRLGFDENGDERAYFIDAYSAAPGGGRVMERSYLADSPVILSYGRLLVIRHGQ
                     VR"
     gene            complement(2083..>2439)
                     /gene="fusA"
                     /locus_tag="PLW80_02980"
     CDS             complement(2083..>2439)
                     /gene="fusA"
                     /locus_tag="PLW80_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684885.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPB65496.1"
                     /translation="IGVKLVGVDYDELAATPFAFEACASMAFDEACRKAGPVQLEPVM
                     KVDIMTPKDYLGDVMSLLSQRGGIINGSDSRAAVDIVHSQAPLSVMFGFSTSLRSVSQ
                     GRASFSMEFSHFEQKR"
BASE COUNT          433 a          743 c          803 g          460 t
ORIGIN      
        1 ccgccctggc caccgtcgcg gaggccgtgc gcacgaagtc gccgtggtcg cgtagcagct
       61 tgaggacgtt cggcgagcgg gcgtgcgcgt tcaggtagcc gaagccgtcc tgaacctcgc
      121 ggcatagctc gaagtacgcg ctcaccgaag ggcggctacc cggcaccgcg cgttcgacct
      181 cggctatgca ggcctcgatg ccgaagggca tgcgcaggtc gaccccgtcg tcggtcagga
      241 tgagcctata ggcctcgggc acgctcgcga agcctacctc gaggccggcg tcgtcgagca
      301 ggtagcgcac gaccccggtc gcctcggaca tggagcgcat atcgggcagc tcgtgcagcg
      361 acggctcgaa ctcgaagcgg ccgcggacga agctcgtggc gaagccgccg ggcaggttgt
      421 gcccttcgag gagtagcgtc ttggctccgg cgcgcgacag ctcgaacgcc gcgcacaggc
      481 ctccgaggcc cgccccgacg acgacgcagt cgtaggcgcc cgtacccgtc agggcggcct
      541 cgtttcccgt atgtgccatc gggacaagta tacacgctat agggattgcc gcaattctaa
      601 tcgaggctcg gctcctcgtc gactcgccgc gaggcctggc gcgggacagc gaccgccggt
      661 tagcgttcgt tacgtgccat cgggattgcc ttcaatctcc ttgactgttt tgaaatatag
      721 tcataatata gtcatataag gagcctttat gcagacaggg aggactgtag ccgttgctga
      781 actcaaagct catctgagcg ccgagctacg gcgcgttcag ggaggcgaga cgaccgtcat
      841 cgtggatcac agaaaaccca tagctctatt gtccggcttg gatccaatgc cgaagtacgc
      901 gcgtagaaga cgcgggcctt tcgtctggcg agagttcgaa cccctgacta caagcgccat
      961 acaggcgctg atcgataagg aacgggcgga ttcgtggtag cgtacctgga ttcgtcggtc
     1021 atgctcaggt atatactgct tggagaaacg gcgcttcgtc atgtccaggc ctttccgcgg
     1081 ctagcttcaa gcgaactgct tgagatcgaa tgccgaagag tcctgttgcg gtgccggatc
     1141 gagggcgaac tgaatgacga gtcactggtc gaggctacga gtcgcttgga tgaggcgctg
     1201 agtggtgtcg accttctaga actcgatgcc gctgtcaaga agcgggctat ggaagctttc
     1261 ccgattcatg tcaaaacgct cgacgcgctg cacctggcga gcgccctgca gttgctcgtc
     1321 gaagaacccg cggatgggat cgtggttttc tcgcacgatc gcacgttcaa cctttgcgcc
     1381 cgagcgctcg gcttcaatgc ggcattactc tgatagtgag acaggacggg ccgataaaac
     1441 gacgggatgc cggagggccg tcgccgtatg ctccacgctc tgtacggctc tgctgttgtc
     1501 gtcttgtcag ttcgcttcgc tcgatgccat cgtcccagcg cccgccttca tacgcgaggc
     1561 ggcttacgcg gaggccgaac gctacctggg catggagtac gagtggggcg ggcaggattt
     1621 ccctcgcggt atagactgct ccggcctcgt cgtcaacgta taccataccg taacggccgg
     1681 tacggagtac gcgccgccat tcgacgacgc cactgccgcg atgctcagcg agcggtatac
     1741 cgtagatatc gatgtgccgg cccgcggaga cctcgtgttc atgggcgacg gcgccgtcag
     1801 ccacgtggcc atcctggatc gcctagggtt tgatgagaac ggcgacgagc gcgcctactt
     1861 catagacgcc tactccgcag ccccgggcgg cggccgcgtg atggagcgta gctacctggc
     1921 cgattcgcct gttattctgt cgtacggtcg gttactggtc atccggcatg gccaagtccg
     1981 ctagcgcgga cttggccggt ccgtggcgcg tcgtcgtccg tgacgttccc gtccgctcgg
     2041 gcggacgggc catggtgtgg cgcgccgtcc gtggcgcgcg tgctaccgct tctgctcgaa
     2101 gtgcgagaac tccatcgaga agctagcccg tccctgtgat acggagcgga ggctggtgga
     2161 gaagccgaac atgaccgaca gcggcgcctg ggagtggacg atgtcgacgg cggctcggga
     2221 gtcggagccg ttgatgatgc cgccgcgctg ggacagcagg ctcatgacgt cgcctaagta
     2281 gtccttgggc gtcatgatgt cgaccttcat caccggctcg agctggaccg ggccggcctt
     2341 gcggcaggcc tcgtcgaagg ccatcgaggc gcaggcctcg aaggcgaagg gcgtggcggc
     2401 cagctcgtca taatcgacgc cgaccagctt gacgccgat
//
LOCUS       DAOOLN010000169         2438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_53493_length_2438_cov_3.245489, whole genome shotgun sequence.
ACCESSION   DAOOLN010000169 DAOOLN010000000
VERSION     DAOOLN010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53493_length_2438_cov_3.245489"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="PLW80_02985"
     CDS             <1..681
                     /locus_tag="PLW80_02985"
                     /inference="COORDINATES: protein motif:HMM:NF022635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HPB65497.1"
                     /translation="PVPAQEAAVAYLEHVASGLGLGPRLRLGGHSKGGNLAVYAASFC
                     RARTQRRVRAVYNNDGPGFDATVIAREGYRTIQERIRSFVPQSSIIGMLLEHHERYTV
                     VQSTESGLMQHDPYSWSVLSTDFITVKQVTDESLFVDRALKEWVGAMAPAQRELFIDA
                     VFDVLSATDAKTFSELGGDWLKSSIAVAKSLRGLDEATRKVMFDALKLLFKAIRENLP
                     AIMQKRPQ"
     gene            778..2046
                     /locus_tag="PLW80_02990"
     CDS             778..2046
                     /locus_tag="PLW80_02990"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPB65498.1"
                     /translation="MRRRPVPLIVPTSAVIAGSYVLALLFFSLAVGASSNHPWFAGTY
                     YGCVAASAALSVLSVALGQAWVSGFIACLRCYLIIVLGYGVSAYLSVKLTLGAGLMVE
                     VCALVPYPANLALCAALGSAIVLAQVFPGFMGASQLVEATARARPDEVLALAALLAMA
                     AGAAALIGRLVSVNDSLGRALRMQERSVDTLAELNKNLQGYARTADEDSAERERARIS
                     REIHDISGYIFTNLIALMDAAGSLPPDDRETLLDLVGTARKQAQEGLQETRAALRKLR
                     DEKPRMADNARAMHKIVSIFRTVAGIDVDLELGNLPRLLPTELNLTLYRAIQEGLTNA
                     VRHGKATAVRIRFWVDDDLVRLSIADNGRGADELTKGIGLSGMEERVGSLGGSVRTGR
                     SPEGGFLLSVDVPLGTGPASTPARKAEATA"
     gene            2043..>2438
                     /locus_tag="PLW80_02995"
     CDS             2043..>2438
                     /locus_tag="PLW80_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB65499.1"
                     /translation="MIRVLIADDQRLFGESLSTTLRNYAKDIDVVGLASDGAEAVELV
                     GRLSPDIVLMDVFMPRLDGVEATRSIMARWPAVRILMLSTYDEDTKVKEALSLGAAGY
                     LLKDITPVVLIASIRALSSGVMQISPQIVA"
BASE COUNT          410 a          845 c          783 g          400 t
ORIGIN      
        1 ccggtaccgg ctcaagaggc ggccgtcgcg tacctggagc acgtcgcctc cgggctcgga
       61 ctcgggccca ggctgaggct cggcggccac tcgaagggcg gcaacctagc tgtctacgcc
      121 gcgtcgttct gccgcgcccg gacccagcgt cgcgtccggg ccgtctataa caacgacggc
      181 cccggcttcg acgccacggt gatcgcccgg gaaggctacc gtactataca ggaacgtatc
      241 cgctccttcg tcccgcagtc gtcgatcatc ggcatgctac tcgagcacca cgagcgctac
      301 accgtcgtcc aaagcaccga gagcggcctc atgcagcacg atccgtactc ctggagcgta
      361 ctcagcaccg atttcatcac cgtcaagcag gtcacggacg agagcctgtt cgtcgaccga
      421 gccctcaagg aatgggtcgg cgccatggcc ccggcccaac gcgagctgtt catcgacgcc
      481 gtcttcgacg tcctgtcggc gacggacgcc aagaccttct ccgagctggg cggcgattgg
      541 ctcaaaagtt ccatagccgt ggcgaagtcc cttcgcggcc tcgacgaggc gaccaggaag
      601 gtcatgttcg acgcgctcaa gcttctattt aaagctatcc gggaaaacct tccggccatc
      661 atgcagaaac ggcctcagta gcatggacac gtcgtcgtcg tcgaccgatg ctcgcgctat
      721 gcgactacca ggttcccccg ccgaacgcca cgagcggatg gcgggatagg ctcgtctatg
      781 cgccgccggc ccgttccgct catcgtcccg acctccgccg tcatcgccgg tagttacgtc
      841 ctggccctgc tgttcttttc cctcgcggtc ggagccagct cgaatcatcc gtggttcgcc
      901 ggcacctact acggctgcgt cgcggcatca gcggcgctca gcgtcctgtc cgtcgccctc
      961 gggcaggcct gggtctcggg cttcatagcc tgcctgcgct gctacctgat catcgtgctc
     1021 ggctacggcg tctcggccta cctgtcggtc aagctgacgc tcggagccgg gctgatggtc
     1081 gaggtctgcg ctctcgtgcc ctacccggcc aacctggccc tctgcgcggc gctcggctcg
     1141 gccatcgtac tggcgcaagt cttcccgggc ttcatgggcg cgagccagct ggtcgaggcc
     1201 acggccaggg cccgcccgga cgaggtcctg gctctcgctg cgctcctggc catggccgcc
     1261 ggggcggctg ccctgatcgg ccgtctcgtc tccgtcaatg actcgctcgg gcgagccttg
     1321 cgcatgcagg aacgctcggt cgacaccctg gccgaactca acaagaacct gcagggctac
     1381 gcccgcacgg cggacgagga ttcggccgag cgagagcgag cccgcatcag ccgcgagatc
     1441 cacgatatct ctggatatat attcacgaac ctcatcgccc tgatggacgc ggcggggagc
     1501 ctgccgccgg acgaccggga aaccctgctc gacctcgtcg gtacggccag gaaacaggcg
     1561 caggaggggc tccaggagac gagggccgcc ctgcgcaagc tacgcgacga gaagccgcgc
     1621 atggccgaca acgcccgggc gatgcacaag atcgtctcga tcttcaggac ggtggcgggc
     1681 atcgacgtcg acctcgagct cggcaacctg ccgaggctgc tcccgaccga actcaacctg
     1741 acgctgtatc gcgccatcca ggaggggctg accaacgcgg tacgccacgg caaggccacg
     1801 gcggtacgca tacgcttctg ggtcgacgac gacctcgtcc gcctctcgat agccgataac
     1861 ggccgcgggg ccgatgagct taccaagggc ataggcctat cgggcatgga ggagcgggtc
     1921 ggctcgctcg gcggctcggt acgcacgggg cggtcgccgg agggaggctt cctgctctcc
     1981 gtcgacgtac cgctcgggac cgggcccgcc tcgacgccgg ccagaaaggc ggaggcgacc
     2041 gcgtgatacg ggtactcatc gctgacgacc agcgcctgtt cggggagagc ctgtcgacga
     2101 cgctcaggaa ctacgccaag gacatcgacg tggtggggct agcctccgac ggggccgagg
     2161 cggtcgagct ggtcgggcgc ctatcgccgg acatcgtgct gatggacgtc ttcatgccca
     2221 ggctcgacgg ggtcgaggcg acgagaagca tcatggcgcg atggccggcc gtccgcatcc
     2281 tgatgctatc cacctacgac gaggatacga aagtcaagga agccctgtcg ctcggggccg
     2341 ccgggtacct cctcaaggac atcacgccgg tagtgctcat agcgagtatc cgggccctgt
     2401 cgagcggcgt catgcagatc tcgccgcaga tcgtcgcc
//
LOCUS       DAOOLN010000170         2419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_54120_length_2419_cov_3.052030, whole genome shotgun sequence.
ACCESSION   DAOOLN010000170 DAOOLN010000000
VERSION     DAOOLN010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54120_length_2419_cov_3.052030"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="PLW80_03000"
     CDS             complement(<1..1083)
                     /locus_tag="PLW80_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPB65500.1"
                     /translation="MSDDMYVCPACGETYSLASMAFRCRCGGLLDLAPFEPRFDPRSS
                     GGERSIFRYASSMPPFSGLEEAWESARSRVTMGEGLTPTVPFGPAEPGLLVKVDYAMP
                     TLSFKDRGAAVLVAAALVAGARRLIQDSSGNAGAAVAAYATRAGLGCDIYTPASTSPR
                     KIAQIEAYGARAVLVPGSREDTAAAALEAAEFGARDGSAFYASHVYNPLFYQGTKTYV
                     YELFETLGGLPDTLYAPLGNGTLVLGMYHALKELRSLGLIVSYPRLVAVQAERCAPVY
                     RASVAGRLSVDPVRNDGTEAEGIAIAAPLRGSQVLAAIRELGGEVAIAPEAGLSPAKK
                     AAAARGFYIETTSAACLAAYFDRRARG"
     gene            complement(1141..1404)
                     /locus_tag="PLW80_03005"
     CDS             complement(1141..1404)
                     /locus_tag="PLW80_03005"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HPB65501.1"
                     /translation="MSGRYGYVLFHTSAAAFRAEKASRESCASASDTLSGARLVPTPR
                     ALSSDCGVSLRFDLAGTDDGGAERAMRVLLDERGIPFDRIAEA"
     gene            complement(1401..>2419)
                     /locus_tag="PLW80_03010"
     CDS             complement(1401..>2419)
                     /locus_tag="PLW80_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPB65502.1"
                     /translation="VAEALGAGDPLRIAFAANATAAINAALLGVLRPGDKVVADSLAH
                     NAVARPLTELGRNGVAVEWIRCGRDGSVDLGHAEALVRGRGGRAARMLIVTHGSNVCG
                     SLAPLGELAAICARHGTTLLVDAAQTAGVEYIGLDSLGDAMVAFTGHKAMLGPGGTGG
                     LALSSRADIERVSPVIRGGTGSASDSEDQPAFMPDRLEAGTTNAPGLAGLAAGLAWIG
                     ANGGRESIREHERRLTENLVSGVAALPGAIVYGPEPGRPRCAVMSFTLDGWSCSDLAL
                     ALDERFGVLCRVGLHCAPRAHRTLGTFPDGTARFAPGPFTTEADVAYALGALAELASE
                     GGRS"
BASE COUNT          389 a          849 c          855 g          326 t
ORIGIN      
        1 gccgcgagcc ctgcgatcga agtaggcggc gaggcaggcg gcgctcgtcg tctcgatata
       61 gaagccgcgc gccgcggcgg cctttttggc cgggctcagc ccggcctccg gtgcgatggc
      121 gacctcgccg cctagctcgc ggatcgctgc caggacctga ctgccgcgaa gcggggccgc
      181 gatggcgata ccctcggcct cggtaccgtc gttccggacc gggtccacgg agagccggcc
      241 ggcgacggag gcgcggtaga ccggagcgca acgctcggcc tgcacggcga cgagcctggg
      301 ataggagacg atgagcccga gcgaacgtag ctccttgagc gcgtggtaca tcccgagcac
      361 cagagtcccg ttgccgagcg gcgcgtagag tgtatcgggt aggccgccca gagtctcaaa
      421 tagctcgtac acataggtct tggtcccctg atagaagaga gggttgtaga cgtggctcgc
      481 gtagaaggcc gagccgtcgc gagcgccgaa ctcggccgcc tcgagcgccg cggcggccgt
      541 atcctcgcgg ctaccgggta cgagcacggc cctggcgccg tacgcctcga tctgggctat
      601 cttgcgcgga gaggtggagg caggcgtata gatgtcgcag ccgaggcccg ccctggtcgc
      661 gtaggcggcc accgccgccc cggcgttgcc cgagctatcc tggatcagcc tgcgcgcccc
      721 ggcgacgagg gcggccgcca ccagcacggc ggctccgcgg tccttgaacg acagggtcgg
      781 catcgcgtag tcaaccttga cgagcaggcc aggctcggcc gggccgaagg gtacggtcgg
      841 cgtgagcccc tcccccatgg tcacgcggct ccgggccgat tcccaagcct cctccaggcc
      901 ggagaaggga ggcatggagg aggcgtagcg aaagatggac cgctcgccgc cggaagatcg
      961 cgggtcgaac cgaggctcga acggcgccag gtcgaggagg ccgccgcaac ggcacctgaa
     1021 ggccatcgat gccagggaat aggtctcgcc gcaggcggga cagacgtaca tatcgtcgct
     1081 catgccgtcg attatcgccg atcacggcga ctataggcaa tcgaattacg accggcgacg
     1141 ttacgcctcg gctatcctgt cgaaggggat gccgcgctcg tccagcagga cgcgcatcgc
     1201 ccgctcggca ccgccatcgt cggtccctgc cagatcgaaa cggagcgaga cgccgcagtc
     1261 gctcgacagc gccctcggcg tcgggaccag cctagccccg gacagcgtat cggacgcgga
     1321 cgcgcaggac tcgcgggagg ccttctcggc cctgaaggcc gcggcgctcg tatggaacag
     1381 cacgtagccg taacgtccgc tcacgagcgg ccgccttcgg acgccaactc ggccagggcg
     1441 ccgagcgcgt aagccacgtc ggcttcagtc gtaaacggcc ccggggcgaa cctggccgtg
     1501 ccgtccggga aggtaccgag agtacggtgc gccctcggcg cgcagtggag gccgacgcgg
     1561 cacaaaacgc cgaagcgctc gtcgagcgct agcgccagat cagagcagct ccagccgtcc
     1621 aaggtgaacg acatgacggc gcatcggggc ctaccgggct ccgggccata gacgatggcg
     1681 ccgggcagcg cggcgacgcc ggagacgagg ttctcggtca gacgacgttc atgttcccgg
     1741 atcgactccc ggccgccgtt cgcgccgatc caggccaggc cggcggcgag cccggcgagc
     1801 cccggcgcgt tcgtcgtgcc cgcctcgagt cggtcgggca tgaaggccgg ctggtcctcg
     1861 ctatcggacg cgctgccggt accgccccgg atgaccggac tgacgcgctc gatatccgcg
     1921 cggctcgaca gggccagccc gcccgtgccg cccgggccga gcatcgcctt gtggcccgtg
     1981 aacgcgacca tggcgtctcc caacgagtcg agcccgatat actccacccc ggcggtctgc
     2041 gccgcgtcga cgagcagggt cgtcccgtgg cgtgcgcata tcgcggccaa ctctccgagc
     2101 ggggccagcg agccgcaaac gttcgaaccg tgcgtgacga tgagcatcct ggccgcgcgg
     2161 ccgccccgac cgcggacaag cgcctccgcg tggccgaggt cgaccgagcc gtctcggccg
     2221 cagcgtatcc actcgaccgc cacgccgttc cgtcctagtt cggtcagcgg gcgcgccacg
     2281 gcgttatggg ccaggctatc ggcgacgacc ttgtctcccg gccgtaggac gccgagcagg
     2341 gccgcgttga tcgccgccgt ggcgttggcc gcgaatgcga tgcgcaacgg atcgccggcg
     2401 ccgagtgcct cggctaccg
//
LOCUS       DAOOLN010000171         2418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_54150_length_2418_cov_2.563267, whole genome shotgun sequence.
ACCESSION   DAOOLN010000171 DAOOLN010000000
VERSION     DAOOLN010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54150_length_2418_cov_2.563267"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PLW80_03015"
     CDS             complement(<1..268)
                     /locus_tag="PLW80_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPB65503.1"
                     /translation="MSGFKPVAAVYGGANIDIQARSRGPYRAGDSNPGAASITHGGVG
                     RNIAENLAGLGVPTELVTVFGGDEQSSSLVEGCRRVGIQVSSSLF"
     gene            341..826
                     /locus_tag="PLW80_03020"
     CDS             341..826
                     /locus_tag="PLW80_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HPB65504.1"
                     /translation="MDQLFDRFGDLLKSWVQSDPGSSRPFSDPFAGKFSKSSGDPFLD
                     DAMDELDAFLNDDRESQERLRREREARARAEERAARPAAASGPPAKLVAAYKTLGLAY
                     GAPFDQVRAAYKRLLKEHHPDRHGSSPEAQLKATETSARINDAYRIIETWRDTGTLGD
                     E"
     gene            896..1330
                     /gene="fliS"
                     /locus_tag="PLW80_03025"
     CDS             896..1330
                     /gene="fliS"
                     /locus_tag="PLW80_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HPB65505.1"
                     /translation="MTQNPLAAYKETRIRTAGPGQLVVMLYEEAVKQCDRAVDFLDTD
                     LKKKPANIEKVNQAICKVQDIVTELMASLDFEAGGDIARDLFSLYVYFNRELTEANIA
                     KDVGRVRAIRAMLAELSSAWAVAATKAQGSGDERRSGVNIAG"
     gene            1339..1815
                     /gene="flgN"
                     /locus_tag="PLW80_03030"
     CDS             1339..1815
                     /gene="flgN"
                     /locus_tag="PLW80_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HPB65506.1"
                     /translation="MPPDGKLSRQELDERVAVLKRFRELLVRQRDKFNEYMSLLERQR
                     TDIESGDVDALVAHVELEQGIVSEIFAVQKVIEPLEDMYRSAYRGAEPEGVAELRSTL
                     SELKDEVVVRNSENRALLKQRMDMLRHEIMSVGNPYAKRKSVYGSAAEPRALDIRG"
     gene            1864..>2418
                     /locus_tag="PLW80_03035"
     CDS             1864..>2418
                     /locus_tag="PLW80_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPB65507.1"
                     /translation="MEPLFLLDAYGIIYRSYFAFISKPLRNPRGDNVSAAFGFFRSLF
                     QLWDRYSPKAFAAVFDSRVPTFRHERFEAYKATRQKTPEDLHAQIPLVEEILGLLGVP
                     TLRADRYEADDIIAAIAARCRDEGRPCYVVSGDKDLLQLVGGTVRALRPDRDQGFVLV
                     GPAEVEAEWGVPPDRILDYLSLTGD"
BASE COUNT          449 a          837 c          739 g          393 t
ORIGIN      
        1 ggaacagcga cgagctgact tggatgccga ctcgcctaca accctcgacc aggctcgacg
       61 actgttcgtc gcctccgaag accgtgacca gctcggtcgg cacgccgaga ccggccaggt
      121 tctccgcaat attgcggccg acgcctccgt gcgtgatgga cgccgctccg ggattcgagt
      181 cgccggcccg gtaggggccc cggctacgcg cctggatatc gatattcgcg ccgccgtaga
      241 cggccgccac cggcttgaac ccgctcatgc tgctcctcgt cgtaccgctc gtcttgcctc
      301 tcggaccggc ttgttgtaga gtatagtccg tatgtccagc atggatcaac tcttcgatcg
      361 attcggcgac ctgctcaagt cctgggttca atcggacccg ggctcgtcgc gccccttctc
      421 agatcccttc gcggggaaat tctccaagag ctccggggat ccgttcctcg acgacgctat
      481 ggacgaactc gacgcgttcc tgaacgacga ccgcgaatcc caggaacgcc tgcgccgcga
      541 gcgcgaggcg cgggctcgcg ccgaggaacg cgccgcgcgt ccggccgccg cctccgggcc
      601 tccggccaag ctcgtcgccg cctacaagac gctcggcctc gcctacgggg ctccgttcga
      661 ccaggtcagg gccgcctaca agcggctgct caaggaacac cacccggaca ggcacggttc
      721 gtcgccggag gcccagctca aggccaccga gacgagcgcg cgcatcaacg acgcctacag
      781 gatcatcgaa acctggcgcg acaccggcac cctgggagac gaatgatttt atgctcaagc
      841 cagcccttcc cgttccgata tataaagaag aagatcagag gagggggaga tccagatgac
      901 ccagaatccg ctcgctgcct acaaggaaac ccgcatacgc accgccggcc cgggccagct
      961 cgtcgtcatg ctgtacgagg aggcggtcaa gcaatgcgac agggcggtag acttcctcga
     1021 tacggacctc aagaagaagc ccgccaacat cgagaaggtc aaccaggcga tttgcaaggt
     1081 tcaggacatc gtcaccgaac tgatggccag cctcgacttc gaggccggcg gcgacatcgc
     1141 ccgcgacctg ttctccctat acgtctattt caaccgcgag ctgaccgagg cgaacatcgc
     1201 caaggacgtc ggccgggtcc gcgcgatcag ggcgatgctg gccgagctgt catcggcctg
     1261 ggccgtcgcg gccaccaagg cccagggctc gggcgacgag cggcgctccg gcgtcaacat
     1321 agccggctag gcgcgcggat gcctcccgac gggaagctgt ccaggcaaga gctggacgag
     1381 cgggtcgccg tactcaagcg cttcagggaa ctgctcgtcc gccaacgcga caaatttaac
     1441 gaatacatgt ccctgctcga gcgccagcgc accgacatcg agtcgggcga cgtcgacgcg
     1501 ctcgtggccc acgtcgagct ggagcagggc atcgtctccg agatcttcgc cgtccagaag
     1561 gtgatagaac cgctcgagga catgtaccgc tcggcctacc gcggcgccga gcccgaaggc
     1621 gtcgccgagc tacgctccac cctatccgaa ctcaaggacg aggtcgtcgt caggaattcc
     1681 gagaaccgcg ctctcctcaa gcagcgtatg gatatgttgc gccacgagat catgagcgtg
     1741 ggcaatccat acgccaagcg gaagagcgtc tacggctcgg ccgcggagcc gcgagccctc
     1801 gacatacggg gctgagcctt actcggcctt gcctgacggg ccgcatgcgc gtatcctgtt
     1861 ccgatggaac cactcttcct cctcgatgcc tacggcatca tctaccgctc ctacttcgcg
     1921 ttcatatcga agccgctacg taacccgcgc ggcgataacg tatccgccgc cttcggcttc
     1981 ttccgaagcc tgttccagtt atgggaccga tactcgccca aggccttcgc ggccgtcttc
     2041 gactcgcggg tgccgacctt caggcacgag cgcttcgagg cgtataaggc gacgaggcag
     2101 aagacccccg aggacctgca cgcccagata ccgctcgtgg aggagatcct cggcctgctc
     2161 ggcgttccaa cgctacgggc ggatcgctac gaggccgacg atatcatcgc cgcgattgcg
     2221 gcccgctgcc gcgacgaggg ccgtccctgc tacgtggtct ccggcgacaa ggacctgctc
     2281 cagctggtcg gcggtacggt cagggcgctc aggcccgacc gcgaccaggg cttcgtgctc
     2341 gtcgggccgg ccgaggtcga ggcggaatgg ggcgtgcccc ccgaccgcat tctcgactac
     2401 ctgtcgctga ccggcgac
//
LOCUS       DAOOLN010000172         2417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_54180_length_2417_cov_2.917019, whole genome shotgun sequence.
ACCESSION   DAOOLN010000172 DAOOLN010000000
VERSION     DAOOLN010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54180_length_2417_cov_2.917019"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..1076)
                     /locus_tag="PLW80_03040"
     CDS             complement(21..1076)
                     /locus_tag="PLW80_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HPB65508.1"
                     /translation="MDQTILDYVVARVVDLCGIPSPTGYTREAEAYVFAELERLGYEP
                     EYTNKRSVRVRLGGSGRPLVLAAHIDTLGAMVRSVKPSGALRVAKLGGFPENYIEGEN
                     CVVHTRGGKRFTGRFLLDKPAVHVNADVGGAKRDDLSLEIVLDEPVSKADDTKALGIG
                     SGDFVSFDPRTVVTESGYIKSRHLDDKASSGVLLALAKMIADGAASPERETWLLFTNF
                     EEVGHGGSAGLPAGVEEFLAVDMGAIGDDLGADDRSVSICAKDSGGPYDLEMTDALIA
                     LARGLELRHAVDVFPFYGSDAGAALKAGYDIRHALIGPGVQASHGYERTHREALRGAL
                     ALAGAYCCGAAAFAPAD"
     assembly_gap    1125..1224
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1242..>2417)
                     /locus_tag="PLW80_03045"
     CDS             complement(1242..>2417)
                     /locus_tag="PLW80_03045"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPB65509.1"
                     /translation="EKNHTITVEAGMSGPELERQLRNAAALHGAKRAYTCGHLPQSFE
                     YSAVGGWVTTRGSGQNSSYYGNAADMVIAQDYVCPACDIRTGEFPAAANGPDLDQMMM
                     GSEGTFGVLYSAALRVRRYMPENTRRYSFIFRDWESAKEAAREVMQGEFGLPSVFRLS
                     DPEESDVALRLYGVSGTPLDAAMTAFGYKPGERCLMLGTADGERGFARHVERRIKRIC
                     RRHGAMGTTSYVTRAWEHGRFRDPYLREDLQDFGIMIDTLECTVTWTNLEEVHTGVRA
                     FCHSRPGTICMTHMSHFYPQGCNLYFIFIAKMDDLDEYLAYQAGILDHIQKFGATMSH
                     HHGIGKAFAPWLEGDVGPEAMAVYRALKRHFDPKGIMNPGGTLALDLPDEQRRLLRK"
BASE COUNT          354 a          751 c          822 g          390 t
ORIGIN      
        1 aatcgcgcaa taccgaagcc ctagtcggcc ggcgcgaagg ccgcggcgcc gcagcagtag
       61 gcgccggcca gggccagggc gccgcgcaga gcctcgcggt gcgtccgctc gtaaccgtgc
      121 gacgcctgta ccccggggcc gatgagcgcg tgcctgatat cgtagccggc cttgagcgcg
      181 gcgccggcgt cggagccgta gaacgggaag acatcgaccg cgtggcgcag ttctaggccg
      241 cgggcgaggg cgatcagcgc gtcggtcatc tcgaggtcgt aaggcccgcc cgagtccttg
      301 gcgcagatcg acacgctgcg gtcgtcagcg ccgaggtcgt cgccgatggc gcccatgtcg
      361 acggccagga attcctcgac gccggcaggc aggccggccg agccgccgtg gccgacctcc
      421 tcgaaattcg taaacaagag ccaggtctct cgttcgggcg aggccgcgcc atcggctatc
      481 atcttggcga gggccaggag cactccggaa ctggccttgt cgtcgaggtg gcggctcttg
      541 atatagcccg actcggtgac gacggttcga ggatcgaacg acacgaagtc gcctgagccg
      601 atgccgagcg ccttggtatc gtcggccttg gagacgggtt cgtcgagcac gatctccagg
      661 ctcaggtcgt cgcgcttggc gccgccaacg tcggcgttga cgtggaccgc cggcttgtcc
      721 aggaggaagc gccccgtgaa gcgcttgccg cccctcgtat gcacgacgca gttctcgccc
      781 tcgatgtagt tctcgggaaa gccgcctagt ttggcgacgc ggagcgcccc ggacggcttg
      841 accgagcgca ccatggcgcc gagcgtgtcg atatgggccg cgagcacgag cgggcggccg
      901 gagccgccga gcctgacccg caccgaacgc ttgttcgtgt actcgggttc gtagccgagc
      961 cgctcgagct cggcgaagac gtaggcttcg gcctcccggg tatagccggt cgggctcggt
     1021 atgccgcaca aatcgacgac gcgcgccacg acgtagtcga gtatcgtttg atccatgatg
     1081 tactcctcgc gcggtattgt accacacgcg gcgaccccgg cggcnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnaccccg gcggccagcg cttacttcct caggagcctc
     1261 cgttgctcgt ccggcaggtc gagcgctagc gtgccgccgg ggttcatgat gcccttgggg
     1321 tcgaagtggc gcttgagggc gcggtagacg gccatggcct cggggccgac gtcgccctcg
     1381 agccagggcg cgaaggcctt gccgatgcca tggtgatggc tcatcgtcgc cccgaatttc
     1441 tggatatggt ccaggatgcc cgcctggtag gccaggtact cgtcgaggtc atccatcttg
     1501 gctatgaaga tgaagtacag gttgcagccc tgcgggtaga agtgggacat gtgggtcatg
     1561 cagatggtgc cgggccgcga gtggcagaat gcgcggacgc cggtatggac ttcctcgaga
     1621 ttggtccagg tgacggtaca ctcgagggta tcgatcatga taccgaagtc ctgcaggtcc
     1681 tcgcgcaggt aggggtcgcg gaagcggccg tgctcccagg cccgcgtgac gtaggaggtg
     1741 gtgcccatgg cgccgtgccg cctgcagatc cgcttgatcc tgcgctcgac gtgacgggcg
     1801 aagccgcgct cgccgtcggc ggtaccgagc atcaggcagc gctcgccggg cttgtagccg
     1861 aacgccgtca tcgccgcgtc gagcggtgtg ccggagacgc cgtacagcct gagcgccacg
     1921 tcggactcct cggggtcgga cagcctgaat acgctcggca ggccgaactc gccctgcatg
     1981 acctcgcggg ccgcttcctt ggcgctctcc cagtcgcgga agatgaacga atagcgcctc
     2041 gtgttctcgg gcatgtacct acggacccgg agcgcggccg agtacaggac gccgaaggta
     2101 ccctcgctgc ccatcatcat ctggtccagg tcggggccgt tggccgcggc ggggaactcg
     2161 cccgtgcgga tgtcgcaggc cgggcagacg tagtcctggg cgatcaccat gtcggcggcg
     2221 ttgccgtagt agctcgagtt ctggcccgag ccgcgggtcg tgacccaccc gccgacggcc
     2281 gagtactcga agctctgcgg caggtggccg caggtgtagg cccgcttcgc gccgtggagg
     2341 gcggcggcgt tcctgagctg gcgctcgagc tcggggcccg acatgccggc ctccacggtg
     2401 atcgtgtggt tcttctc
//
LOCUS       DAOOLN010000173         2413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_54300_length_2413_cov_3.905852, whole genome shotgun sequence.
ACCESSION   DAOOLN010000173 DAOOLN010000000
VERSION     DAOOLN010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54300_length_2413_cov_3.905852"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..1025
                     /locus_tag="PLW80_03050"
     CDS             75..1025
                     /locus_tag="PLW80_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HPB65510.1"
                     /translation="MKKISILLIAIALVAALSGCGAPKQSFVSIASGGTGGTYYPLAG
                     AMATIWNEGVKGMNATAQATGASVANVNLLREGKADVIMVQNDIAYYAVNGLELFKDK
                     AFPELKGMAILYPETCQLITLANSGINSVAGLKGKKVAVGAAGSGVEANARQIMEAAG
                     VTYADIQVQYLSFNEAASNLKDGNIDAAFLTAGHPTAAVQDISATKDIRVVKIDDAVV
                     AKLQSTYPFYTKTVIPANTYKGVDADTQTVAVQAMLAVSASLDAATVEKMLQTMFSAA
                     GTERLTAAHAKAGAMIKLENAQAGMSLPLHPGAEKFYGKK"
     gene            1073..1522
                     /locus_tag="PLW80_03055"
     CDS             1073..1522
                     /locus_tag="PLW80_03055"
                     /inference="COORDINATES: protein motif:HMM:NF020479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HPB65511.1"
                     /translation="MRYRSAAVALACAASLLTRFGPARALRIRDEAGRPLATIAVPAT
                     FTLSFMHSVNLSRVDEEFSVGGGGLTLERVLFDQLSTGMPSGDEDGFCVVDGRFETRP
                     GRRFDEIAVRVSPIPGHELRVDGRVRPLTYWAPTGNMVRFSASRESD"
     gene            1541..>2413
                     /locus_tag="PLW80_03060"
     CDS             1541..>2413
                     /locus_tag="PLW80_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HPB65512.1"
                     /translation="MSESTTNDAIATAEADSQAILKKYDKESNFRVYSGLMAKVISAI
                     AITFTCFQLYTALFGVLDAMLQRAVHLAFGMALIFLLYPTSKKWSKDRLHPLDLGLAV
                     LGAITPMYIIANYGQLVLRAGTATTMDMIVGVVGILLVLEAARRIVGLPIVIIAGVFI
                     AYAFAGPWLPGQLAHRGVDVGDLVQHLYFTTEGVFGIPLGVSSTFIFLFILFGAYLEK
                     TGLGQYFIDLANSIAGRSAGGPAKVAVLSSGLMGTVSGSSVANVVGTGSFTIPMMKRL
                     GYKPEFAGAVEATAS"
BASE COUNT          446 a          843 c          720 g          404 t
ORIGIN      
        1 ttcccttgcc gcgtgcatag gaacagtacc atactgcatt catatccacc ggcactcaac
       61 aaggaggaac tacaatgaag aagatcagca tactcctgat cgcgatcgcg ctcgtcgccg
      121 ccctctcggg ctgcggagcc cccaagcagt cattcgtatc catcgcttcc ggcggcaccg
      181 gcggcaccta ctacccgctc gcgggcgcca tggcgaccat ctggaacgag ggcgtcaagg
      241 gcatgaacgc caccgcccag gcgaccggcg cctcggtggc caacgtcaac ctgctccgcg
      301 agggcaaggc cgacgtgatc atggtgcaga acgacatcgc ctactacgcc gtcaacggcc
      361 tcgagctgtt caaggacaag gccttccccg agctgaaggg catggccatc ctctatcccg
      421 aaacctgcca gctgatcacg ctcgccaact ccggcatcaa ctccgtagcc gggctcaagg
      481 gtaagaaggt agccgtgggc gccgccggtt caggcgtcga ggccaacgcc cgccagatca
      541 tggaagccgc cggcgtcacc tacgccgaca tccaggttca gtacctgtcg ttcaacgagg
      601 ccgccagcaa cctgaaggac ggcaacatcg acgcggcctt cctgaccgcc ggtcacccga
      661 ccgccgccgt ccaggacatc tccgcgacca aggacatcag ggtcgtcaag atcgacgatg
      721 ccgtcgtcgc gaagctccag tcgacctacc ccttctacac gaagacggtc atcccggcga
      781 acacctataa gggcgtagac gccgacaccc agaccgtggc cgtccaggcc atgctcgccg
      841 tctccgcctc gctcgacgcc gccaccgtcg agaagatgct ccagacgatg ttctcggccg
      901 ccggtaccga gcgcctgacc gccgcccacg ccaaggccgg cgcgatgatc aagctcgaga
      961 acgcccaggc cggcatgtcg ttgccgctgc atccgggcgc cgagaagttc tacggcaaga
     1021 agtaacgaga ggaaggagcc ggcggcccat cgatggggcg ccggcgactc gtatgagata
     1081 ccgtagcgcg gccgtcgcgc tcgcgtgcgc ggcctcgcta ctgaccaggt tcggccccgc
     1141 tcgggcactg cggatccgcg acgaggcggg gcgcccgctc gcgacgatag cagtcccagc
     1201 caccttcacc ctatccttca tgcactcggt caacctgtcc cgcgtggacg aggagttctc
     1261 cgtgggcggc ggcggcctga cgctcgagcg cgtgctgttc gaccagctgt cgaccggcat
     1321 gccgtcgggt gacgaagacg ggttctgcgt cgtggacggg cgcttcgaga cgaggccagg
     1381 caggcgcttt gacgagatag ccgtccgggt ctcgccgatc cccggccacg agctacgggt
     1441 cgacggccgc gtgcggccac tgacctactg ggcgcccacg gggaacatgg tacgattcag
     1501 cgcttcgcgc gaatccgatt aaccttaagg agatcgatcg atgagtgaat cgacgacgaa
     1561 cgatgcgatc gcgacggcag aagccgatag ccaagcgatc ctcaagaagt atgacaagga
     1621 atcgaatttc cgcgtctact cgggcctcat ggccaaggtg atctcggcca tagccatcac
     1681 cttcacctgt tttcagctgt acaccgccct gttcggcgta cttgatgcga tgctccagcg
     1741 cgcggtccac ctcgcgttcg gcatggcgct gatcttcctg ctctacccga ccagcaagaa
     1801 atggtccaag gaccggctcc acccgctcga cctgggcctc gcggtactag gcgccatcac
     1861 gcctatgtac atcatcgcca actatgggca gctcgtactg cgcgccggca cggcgacgac
     1921 gatggacatg atcgtcggcg tcgtaggcat cctgttggta ctcgaggcgg ccaggcgcat
     1981 cgtcggactg cccatcgtca tcatcgcggg cgtcttcata gcctacgcct tcgccggccc
     2041 ctggcttccc ggccagctgg cgcaccgagg cgtcgacgtc ggagacctgg tgcagcacct
     2101 gtacttcacc accgagggcg tcttcggcat accgctcggc gtgtcgagca ccttcatctt
     2161 cctgttcata ctgttcggcg cctacctcga gaagaccggc ctcggtcagt acttcatcga
     2221 cctggccaac tcgatagccg gccgctccgc cggcggcccc gcgaaggtcg ccgtcctgtc
     2281 gagcggcctg atgggtacgg tctcgggcag ctcggtcgcc aacgtcgtcg gcacgggctc
     2341 ctttactatc cccatgatga agcgcctggg ctacaagccc gaatttgccg gagccgtcga
     2401 ggccaccgcg tcg
//
LOCUS       DAOOLN010000174         2408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_54466_length_2408_cov_3.001700, whole genome shotgun sequence.
ACCESSION   DAOOLN010000174 DAOOLN010000000
VERSION     DAOOLN010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54466_length_2408_cov_3.001700"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PLW80_03065"
     CDS             complement(<1..348)
                     /locus_tag="PLW80_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPB65513.1"
                     /translation="MILSDFSVKHPAVVTILLAGLVVFGLIAGSSLNSEMIPPVGLPI
                     ATVLTVYPGAGAKDVERDITRKIENQLSSLAGVTEMSSSSADSYSTVSLEFRADVDVG
                     SKLPQIRELLNGIA"
     gene            complement(345..1427)
                     /locus_tag="PLW80_03070"
     CDS             complement(345..1427)
                     /locus_tag="PLW80_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPB65514.1"
                     /translation="MEANSRARRGPAIMTLRWLVPLALVAGATAFWLISSGREPERPE
                     APPVPVRVMRPAYGDLVKTLKLNAHVESETMVTVVPLVSGILQELPVEVGQTVAAGQV
                     VARIDAQRFELQLKQAEAAYLSAKSSYERVGQLYRANAATQQSYEQAKGQYEAYSSQY
                     ELARIQLDYASVKSPIAGVVLVKHLSAGSIAAPERPLVTIGDLGDLVVRARVPERYYE
                     SFGSIRESMRIAIRREGGDEYPGVIRSVSPFVSAETKNFEVVVSIGGSPELLRPGMFV
                     SVVFELARWPGVHTLPYEALSGDGRLWWVDDGLARSLPFAPDAASDAAFAVPREWAAR
                     DVIVEGQYFASEGSPVTVVAPQEADR"
     gene            complement(1437..2054)
                     /locus_tag="PLW80_03075"
     CDS             complement(1437..2054)
                     /locus_tag="PLW80_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB65515.1"
                     /translation="MTMLDTTRERILEAATAIIRERGPEGLTVEAAADRAGVSRKTVY
                     NHFDGKYALIDVAAAAWMDGLLADLGVIAADASLPFVTKLNAIVERAYAELKNQRRAG
                     AAGSMAASLDLPGFRADLQARLHDFIAGIVQDAIDEGLVRDDFDARRLTYVIINIVSG
                     LAVLAGIEDEPFTRVDILTDSLKALVGGILTPTGAEAMRGSPLLG"
     gene            2207..>2408
                     /locus_tag="PLW80_03080"
     CDS             2207..>2408
                     /locus_tag="PLW80_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HPB65516.1"
                     /translation="METRIEAVQLSWTDGANIIVGQSHFIKTVEDIAEIMAASVPGAR
                     YGLAFNEASGPCLVRTAGNDEGL"
BASE COUNT          411 a          789 c          794 g          414 t
ORIGIN      
        1 cgcgatgccg ttcagtagct cgcggatctg cggcagtttg gaaccgacat cgacatcggc
       61 gcggaactcg agggataccg tcgagtacga gtcagcgctt gacgacgaca tttcggtgac
      121 gccggccagg ctggatagct ggttctcgat cttcctggtg atgtcccgct ccacgtcctt
      181 ggcgccggcg ccgggataga ccgtcagcac ggtcgcgatc ggcaggccga ccggcggtat
      241 catctcggag ttcagcgacg agccggcgat caggccgaat accaccaggc cagccagcag
      301 gatcgtgacg acggcggggt gcttgacgga gaaatcggac agtatcatcg gtcggcttcc
      361 tgtggcgcca ccacggtcac cggcgagccc tcgctcgcga agtactggcc ctcgacgatc
      421 acgtcgcgag ccgcccattc ccggggcacc gcgaacgctg cgtccgacgc cgcgtccggc
      481 gcgaagggca ggctcctggc caggccgtcg tcaacccacc agagccgtcc gtcgccggac
      541 agcgcctcgt agggcagggt atggacgcca ggccaccggg ccagctcgaa cacgaccgat
      601 acgaacatgc ccggccggag tagctctggc gagccgccta tcgacacgac gacctcgaag
      661 ttcttcgtct cggcggacac gaaggggctg acgctgcgga tgacgccggg gtactcgtcg
      721 ccgccctcgc gccgtatcgc tatgcgcatg gactcgcgta tggagccgaa ggactcgtag
      781 tagcgctccg gcacgcgggc ccgcacgacc aggtcgccca ggtcgcctat cgtcacgaga
      841 gggcgctcgg gcgccgctat gctgccggcg ctcaggtgct tgacgagcac cacgccggct
      901 atcgggctct tgacgctcgc gtagtcgagc tggatgcggg ccagctcgta ctgggacgag
      961 taggcctcgt actggccctt ggcctgctcg tagctctgct gggtcgcggc gttggcgcgg
     1021 tagagctgac cgacgcgctc gtacgacgac ttggccgaca ggtaggccgc ctcggcctgc
     1081 ttgagctgta gctcgaagcg ttgcgcgtct atcctggcca cgacctgccc ggcggccacc
     1141 gtctgcccga cctcgaccgg cagctcctgc aggatgcccg agacgagcgg gaccacggtg
     1201 accatcgtct cggactcgac gtgggcattc agcttgagcg tcttgaccag gtcgccgtag
     1261 gcgggacgca tgacgcggac cggtaccggc ggcgcctcgg gccgctccgg ctcccggccg
     1321 gacgatatga gccagaacgc cgtagccccg gcgacgagcg ccaatggcac gagccagcgg
     1381 agcgtcatga tggccggacc gcggcgcgcg cggctattcg cttccatcgt cgccccctat
     1441 ccgagcagcg gcgagccgcg catggcctcg gctccggtag gcgtcaggat accgccgacc
     1501 agagccttaa gcgaatcggt caggatgtcg acgcgcgtga acggctcgtc ctcgatgccg
     1561 gctagtaccg ccaggcccga gacgatgttg atgatcacgt aggtcagccg gcgcgcgtcg
     1621 aagtcgtctc gcacgagccc ctcgtcgatg gcgtcctgca cgatgcccgc gatgaagtcg
     1681 tggagccgag cctggaggtc ggccctgaac cccggcaggt ccaggctggc ggccatcgag
     1741 ccggccgctc cggcgcgccg ctgattcttg agttcggcgt aggcgcgctc gacaatggcg
     1801 ttgagcttgg tgacgaaggg tagcgacgcg tctgcggcta taacccccag atcggcgagc
     1861 aggccgtcca tccacgcggc cgcggccacg tctatcagcg cgtacttgcc gtcgaaatgg
     1921 ttgtatacgg tcttacggct tacgccggcg cggtcggcgg cagcctcgac cgtcaacccc
     1981 tcagggccgc gctcgcgtat gatcgccgtc gccgcctcca ggatcctctc tctcgtcgta
     2041 tcgagcattg tcatcgtcct tccacatatt acacagctat tgtgtaatat gttaccttca
     2101 agtacacatt tcaatagctt ttgtgtaaat tcgatgaaaa gctcgagcaa acccgagctt
     2161 gcggctaaag cgctaagcgg atagactggc tggccggagg atgcatatgg agacgaggat
     2221 cgaggccgtc caactctcgt ggacggacgg cgctaacatc atcgttggtc agtcgcactt
     2281 catcaagacg gtcgaagata ttgctgaaat catggctgcc tcggtcccgg gggccaggta
     2341 cgggctagcc ttcaatgagg cctcagggcc ttgcctggtc aggaccgcgg gtaacgacga
     2401 ggggctcg
//
LOCUS       DAOOLN010000175         2406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_54532_length_2406_cov_3.421523, whole genome shotgun sequence.
ACCESSION   DAOOLN010000175 DAOOLN010000000
VERSION     DAOOLN010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54532_length_2406_cov_3.421523"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..1559)
                     /locus_tag="PLW80_03085"
     CDS             complement(156..1559)
                     /locus_tag="PLW80_03085"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HPB65517.1"
                     /translation="MILLKDCFRVVRPRYGAGPAAGSVGDDLSGVDVLIDGNRIAKIG
                     KALPVPAGACKVVDASRHVVMPGLVNTHHHFYQTLTRNVPAVQDAKLFDWLVYLYEVW
                     KGIDEEAVYWSSMLAMAELAKTGCALSTDHHYLYPRSFTGDLPGIQFKAAADIGLRFA
                     PTRGSMSRSKKDGGLPPDSVVQDEDEILAQSEEALRKYHNAAPDAMRKVALAPCSPFS
                     VSEKSMRDAADLARRYGARLHTHLAETNDEDDFCVQMYGRRPLKVMEDCGFVGKDVWY
                     AHGIHFNDDELDYLARTGTGVAHCPSSNMRLGSGICRVREMLDRGVNVGLAVDGSASN
                     DASDMLGEARQALLLQRVRYGSGGVTAREVLGVATIGGARILGYDDAGSMEPGALADV
                     ALFDVMKLEYAGALSDPAAALLFSGYNHGVDHLVVNGEMVVENGRLVGVDEDRIRDEA
                     NRCAKSLYKKAGIEAAW"
     gene            complement(1556..>2406)
                     /locus_tag="PLW80_03090"
     CDS             complement(1556..>2406)
                     /locus_tag="PLW80_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-5-phosphate-dependent protein subunit
                     beta"
                     /protein_id="HPB65518.1"
                     /translation="AVIFNQFDQLPNHLWHYSVTGPAMDDVFKKMAKAGDHLALTMYS
                     SGSAGTLGSGTYLKEHHPGTRVGVGEALQCPTILENGYGGHRIEGIGDKHIPWIHNLR
                     DTDVAVGVDDEITIRLLRLFNEPAGKKVLAEAGVPQATLDKLDLMGISGIGNLVATIK
                     AAKYYELGADDVALTVFTDSLALYGSRLLELAAERGPYDQRQADRDLDMLRNLSVDYV
                     LDMNHVDRRRVHQLKYYTWIEQLGKDLTELNAQWQDYRAYWGGLHEQADVIDGMIKEF
                     NAEVLH"
BASE COUNT          348 a          784 c          847 g          427 t
ORIGIN      
        1 ggagcgcggg agcaccgacg ccaggtcgac gacgcgctcc ggcttgtccc tggcctcgaa
       61 ggccagcgcg aaggtcccgc cggcgagcgc gagcgctccc ggccgggcga gcaggtcgag
      121 ggcctcgccg aggctctcgg gcctgaaata ttcttttacc atgcggcttc gatccccgct
      181 ttcttgtaga ggctcttcgc gcaacggtta gcctcgtcgc gtatccgatc ctcgtcgacg
      241 cctacgaggc gcccgttctc gacgaccatc tcgccgttga cgacgaggtg gtcgaccccg
      301 tgattatatc ctgagaatag cagcgcggcc gccgggtcgg aaagcgcgcc cgcgtactcg
      361 agcttcatca cgtcgaacag cgccacgtcg gccagggcgc ccggctccat agagccggcg
      421 tcgtcgtagc cgaggatgcg cgcgccgcct atcgtcgcga cgccgagcac ctcgcgggcg
      481 gtgacgccgc ccgagccgta gcgcacgcgc tgcagcagca acgcctgcct ggcctcgccg
      541 agcatgtccg aggcgtcgtt cgacgcggag ccgtcgaccg cgaggccgac gttgacgccg
      601 cggtcgagca tctcgcgcac gcggcagatg ccggagccga gcctcatgtt cgacgacggg
      661 cagtgagcga cgccggtgcc cgtcctggcc aggtagtcga gctcgtcgtc gttgaagtgt
      721 atgccgtggg cgtaccagac gtccttcccg acgaagccgc agtcctccat caccttgagc
      781 gggcggcggc cgtacatctg cacgcagaag tcgtcctcgt cgttcgtctc ggccaggtgg
      841 gtatggaggc gcgctccgta cctgcgcgcc aggtcggccg cgtcgcgcat cgacttctca
      901 gagaccgaga acggcgagca cggcgcgagc gctaccttgc gcatcgcgtc gggcgccgcg
      961 ttgtggtact tcctgagcgc ttcctcgctc tgggcgagga tctcgtcctc gtcctgtacg
     1021 acgctgtccg gcgggaggcc gccgtccttc ttggagcgcg acatcgagcc gcgggtcggc
     1081 gcgaagcgca ggcctatgtc ggcggccgcc ttgaactgga tgccgggcag gtccccggtg
     1141 aacgagcgcg ggtacaggta gtggtggtcg gtcgagaggg cgcatccggt cttggctagc
     1201 tccgccatcg cgagcatgct cgaccagtag acggcttcct cgtcgatgcc cttccagacc
     1261 tcgtagaggt agacgagcca gtcgaatagc ttggcgtcct ggacggccgg cacgttgcgc
     1321 gtcagcgtct ggtagaagtg gtggtgcgtg ttgacgagtc cgggcatgac gacgtggcgg
     1381 ctcgcgtcga cgaccttgca ggcgccggcc gggaccggga gcgccttgcc gatcttggcg
     1441 atgcgattgc cgtctatgag cacgtctacg ccggacaggt catccccgac ggaaccggcc
     1501 gcggggccgg cgccgtagcg ggggcggacc accctgaagc agtccttgag cagtatcaat
     1561 gcaagacctc cgcgttgaat tccttgatca tgccgtcgat gacgtcggcc tgctcgtgga
     1621 gcccgcccca gtaggcgcgg tagtcctgcc actgcgcgtt cagctcggtc aggtccttgc
     1681 ccagctgctc gatccaggtg tagtacttga gctggtgcac gcggcgcctg tcgacgtggt
     1741 tcatgtcgag cacgtagtcg acggacaggt tgcggagcat gtccaggtcg cggtcggcct
     1801 ggcgctggtc gtacgggccg cgctcggccg ccagctcgag cagcctggag ccgtagaggg
     1861 cgagcgagtc ggtgaacacc gtcagcgcga cgtcgtcggc gccgagctcg tagtacttgg
     1921 ccgccttgat cgtggcgacc aggttgccga tgccggagat gcccatcagg tcgagcttat
     1981 cgagcgtggc ctgcggtacg cccgcctcgg cgaggacctt cttgccggcc ggctcgttga
     2041 agagcctgag cagcctgatc gtgatctcgt cgtcgacgcc gacggccacg tcggtgtcgc
     2101 gcaggttgtg gatccacggg atgtgcttgt cgccgatgcc ctcgatgcgg tggccgccgt
     2161 agccgttctc aaggatcgtc gggcactgga gggcctcgcc gacgccgacg cgggtgccgg
     2221 ggtggtgctc cttgaggtag gtgccggagc cgagggtgcc ggccgagccg gaggagtaca
     2281 tcgtcagcgc caggtggtcg cccgccttgg ccatcttctt gaacacgtcg tccatcgccg
     2341 ggccggtcac cgagtagtgc cacaggtggt tcggcagctg gtcgaactgg ttaaagatta
     2401 cggcgt
//
LOCUS       DAOOLN010000176         2397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_54841_length_2397_cov_2.768574, whole genome shotgun sequence.
ACCESSION   DAOOLN010000176 DAOOLN010000000
VERSION     DAOOLN010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54841_length_2397_cov_2.768574"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="PLW80_03095"
     CDS             <1..1113
                     /locus_tag="PLW80_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPB65519.1"
                     /translation="MTTTETSVRPERPSSRSPWIPASAAYEPRRPDPRIRWFLDANEG
                     RPSPSVVAAIGEALSGATSGVGRYPSFYELESAVAAAWGVEPARVVATAGGDDAISRV
                     VAARLAPGAKMLVHEPAFEMFGAYAASRGGVTLALRWMDGESFPLEATVEAIERCPSL
                     GLVTVVSPSNPTGGIVPEADALTIAAACARSGAAMLFDAAYGEFAETDPSARLVAEGS
                     AYVVRSFSKAYGLAGLRIGYVIAPDAPSAASLRACGMPYPSSGLACAAAIAALADRSA
                     MLSAVATARAERDAMAARFRALGARVADSGANFVLARVRDPAALAEAMARAGVAVRGY
                     GGRAGLADAVRVSCPCDADGLAAVLGALDGAEEYIR"
     gene            1110..1712
                     /locus_tag="PLW80_03100"
     CDS             1110..1712
                     /locus_tag="PLW80_03100"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HPB65520.1"
                     /translation="MNDTRSPRAAVVERRTKETDVRVELTLARGPVDVRTGVGFLDHL
                     LSSLAFHGGLSLSLRCDGDIGVDDHHSAEDCALALGSALKDALGDKVGIRRFGSAFAP
                     LDESLARAVVDLSGRPCAVVDLGLTREALGGLACENVAHVVASLANAAMATIHLDVLR
                     GSNDHHRAEAAFKALALALREAIALDGTYGAAVPSAKGAL"
     gene            1709..>2397
                     /gene="hisH"
                     /locus_tag="PLW80_03105"
     CDS             1709..>2397
                     /gene="hisH"
                     /locus_tag="PLW80_03105"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HPB65521.1"
                     /translation="MSAALGQDVIIVDTGRSNAASLAAALERVGARPRLSTDPAAVRV
                     ASFAVLPGVGAFGAVMDRLRAMGVVEALKERVELGLPTLGACLGMQLFFGSSEESPGA
                     VGIGALPGAITAFGPGVRSPQFGWNRLSPPEPSVGGLLAGAEALGGRAACAYFANSYK
                     LDEAPVGWASATAYYGAPFVAAMARGPVLLCQFHPELSGAWGQALLERWLVAGGAPAS
                     APTESSSITAEP"
BASE COUNT          308 a          887 c          838 g          364 t
ORIGIN      
        1 atgacgacga cagagacctc cgtccggcca gaacggccgt cgtcgcgctc tccgtggata
       61 ccggcaagcg ccgcctatga gccgaggcgg ccagacccgc gtatccgttg gttcctcgac
      121 gccaacgagg gtcggccgtc gccctcggtc gtcgccgcga taggcgaggc gctatcgggg
      181 gcgacgtccg gagtcggccg ctacccgtcg ttctacgagc tggaatccgc ggtggcggcc
      241 gcctggggcg tcgagccggc gagggtcgta gctacggccg gcggagacga cgccataagc
      301 cgcgtcgtcg cggcgcgcct cgcgccaggc gccaagatgc tcgtccacga gccggccttc
      361 gagatgttcg gcgcctacgc cgcttccagg ggcggcgtta cgctcgctct acgttggatg
      421 gacggcgaat ccttcccgct ggaagccacc gtagaggcta tagagcgttg cccgtcgctc
      481 gggctggtta ccgtcgtctc cccgtccaac cccacgggcg ggatcgtccc cgaggctgac
      541 gcgttgacta tcgcggccgc ctgcgccagg agcggcgcgg ccatgctgtt cgacgcggcc
      601 tacggggaat tcgccgaaac cgacccgagc gctcgcctgg tagccgaagg ctcggcctac
      661 gtcgtgcgca gcttctccaa ggcctacggg ctagccggcc tgcgcatcgg ctacgtcata
      721 gcgccggatg ccccgtccgc cgcctcgctt cgcgcctgcg gcatgccgta cccgagctcc
      781 ggcctggcct gcgcggccgc catcgcggct ttggccgacc ggtccgccat gctatccgcg
      841 gtcgcgaccg cgcgcgccga gcgagacgcc atggccgccc gcttccgcgc tctcggcgcc
      901 cgggtcgccg attcaggcgc caacttcgtg ctggcgcgcg ttcgcgatcc ggccgccctc
      961 gccgaagcga tggcccgcgc gggcgtcgcc gtcaggggct acggcggccg agcggggctc
     1021 gctgacgccg tccgcgtcag ctgcccctgc gacgctgacg gactggccgc cgtactcggg
     1081 gccctggacg gggccgagga gtatatccga tgaacgatac ccgaagtcct cgcgccgcgg
     1141 tcgtcgagcg gcgcaccaaa gagacggacg tacgggtcga gcttacgctc gcgcgcggcc
     1201 cggtcgacgt gcgtaccggc gtcggcttcc tcgaccacct cctatcgagc ctcgcgttcc
     1261 acggcggact cagcctgtcc ttacgctgcg acggcgacat cggggtcgac gaccaccaca
     1321 gcgccgagga ctgcgcgctg gcgctcggct cggcgctcaa ggacgcgctg ggcgacaagg
     1381 tcggcatacg ccgattcggt tcggccttcg cgccgctcga cgaatcgctg gcgcgcgccg
     1441 tcgtcgacct gtcgggccgg ccctgcgccg tcgtcgacct cggcctgacc cgcgaggccc
     1501 tcggcggcct ggcctgcgag aacgtcgccc atgtcgtggc gagcctcgcg aacgccgcca
     1561 tggcgacgat acacctggac gtgctacgcg gctcgaacga ccaccaccgg gccgaggcgg
     1621 ccttcaaggc gctcgccttg gccctgcgcg aggccatagc gctcgacggc acgtacggag
     1681 ccgccgtacc gagcgccaag ggggccctat gagcgccgcc ttaggacagg acgtcatcat
     1741 cgtcgacacg ggacggtcga acgcggccag cctggccgcc gctctcgagc gggtgggcgc
     1801 taggccgagg ctgagcaccg atcccgcggc cgtccgggtc gcttctttcg cggtgctacc
     1861 cggcgtcggg gccttcggcg ccgtcatgga ccggctgcgc gccatgggcg tagtcgaggc
     1921 gctcaaggaa cgagtcgagc tcggcctacc gacgctcggc gcctgcctgg gcatgcagct
     1981 gttcttcggc tccagcgagg agtcgcctgg cgccgtgggc attggcgcgc ttcccggcgc
     2041 catcaccgcg ttcgggcccg gcgtccgctc gccgcagttc ggttggaacc gcctgtcgcc
     2101 accggagcct tcagtcggcg gtctgctggc tggtgcagaa gcgctcggcg gaagggccgc
     2161 gtgcgcctat ttcgccaact cctacaaact cgacgaggcg cccgtcggct gggcctccgc
     2221 gaccgcgtac tacggcgcgc cgttcgtcgc ggcaatggcg cgcggccccg tgttgctctg
     2281 ccagttccac cccgaattgt ccggggcctg gggccaggcc ctgctcgagc gctggctggt
     2341 cgcgggaggc gctccagcga gcgctccgac cgagtcgtca tcgatcacgg cggaacc
//
LOCUS       DAOOLN010000177         2395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_54903_length_2395_cov_2.910684, whole genome shotgun sequence.
ACCESSION   DAOOLN010000177 DAOOLN010000000
VERSION     DAOOLN010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54903_length_2395_cov_2.910684"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PLW80_03110"
     CDS             <1..527
                     /locus_tag="PLW80_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HPB65522.1"
                     /translation="FDLKRHQEYSGKDLQYIDQDNGNERFVPYIIETSAGLTRQLLMV
                     LCDAYEEEKVADKGNDDDWRTVMKFHPELAPITVAVMPLMKKDGLAELAQEIRSELKH
                     DYATDYDQSGAIGRRYRRQDEAGTPYCVTIDYDSKENGTVTVRDRDTMEQERVHKDEL
                     SAYIRNKIKNYVRK"
     gene            755..>2395
                     /locus_tag="PLW80_03115"
     CDS             755..>2395
                     /locus_tag="PLW80_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65523.1"
                     /translation="MVRKLLCLVAFVCVCASAYAGPPRMAIGDFTVTSDNPKLKYVGK
                     GFAEMIGAELALSKGVVLIDRAKRDQLLGELEFSLSGLSDSDSMLRAGELLAADYLLF
                     GDLVDLGDAVLVSCKMVKTETGEVAWQDKYLGPLADYDGISKSLAASALRGLGATTAV
                     AVVSRPSPQLTVVPPEKKEQALLAFSGAVEALDQNDTEAAKVQLAVARAIDPANAAVT
                     AYLAKLVTNTTKFKIQLESYVSYLNPAFLGIIRQDSIHLVTGMPVWPVYYMPLVEGLN
                     YTSYGDDRYVSETVSASQFGYSLPLGDAFGVRLSGHFAALDVRTRVGSPPISDGSGGQ
                     WSNNNSARGAIGGGLDAGVRLGDAFAIGAGFAYFARSDQDTSPIGPIVKNGGTTWAAD
                     LGFLVRNADESLVWDTRASYIAETQDLVDPLTLALTGETQPFPLGIENNVTFSLLGRK
                     LFIILKNSESIALGDTMISSSLMPAAEYFLLPWLSLRCGVEGTMLVMDGLEPQFGLGG
                     MGTQLRQDTLRLGLVNVDADLAIAPAHGIGAGMLTEHQL"
BASE COUNT          474 a          807 c          693 g          421 t
ORIGIN      
        1 acttcgacct caagcgccac caggagtact ccggcaagga cctccagtac atagaccagg
       61 acaacgggaa cgagcgcttc gtgccgtaca tcatcgagac ctcggccggt cttacccggc
      121 aactcctcat ggtcctctgc gacgcctacg aggaggagaa ggtggccgac aagggcaacg
      181 acgacgattg gcgcaccgtc atgaagttcc acccggagct ggcgcccatc accgtcgcgg
      241 tcatgcccct gatgaagaaa gacggcctcg ccgagctggc ccaggagata cgctccgagc
      301 tcaagcacga ctacgccacc gactacgacc agtcgggcgc catcggccgc cgataccgcc
      361 gtcaggacga ggccggcact ccgtactgtg tcaccataga ctacgactcc aaggagaacg
      421 gcaccgtcac cgttcgcgac cgcgacacca tggagcagga gcgggtgcac aaggatgagc
      481 tgtccgccta tatcaggaat aagataaaga actacgtaag gaaataaggc tgggcgcgac
      541 ccggctacgc cgggtcgggc tctccgctcc aactcctcgt gaagaaggaa aggcgccgag
      601 aggcgccttt ccttcttcgc tccggggttt ccgctccgat ccctcgcgcg gcgccggccc
      661 gcctgacttc tggttcgcga cgcctcgata aggctacgaa ccattgcaaa gacgccgagc
      721 ggccatatac ttagcctccg gaatggaggc tagtatggtt cgcaagctcc tgtgcctcgt
      781 cgcgttcgtc tgcgtctgcg cctcggccta cgcagggccg ccccgtatgg ccatcggcga
      841 ctttaccgtt acgtctgaca atcccaagct caagtatgta ggtaagggct tcgccgagat
      901 gataggcgcc gaactggcct tgtccaaggg cgtcgtgctc atcgaccgag ccaagcgcga
      961 ccagctcctc ggggagctcg agttctcgct ctcgggcctg tccgactcag actccatgct
     1021 cagggcaggc gagctcctgg ccgccgacta cctcctcttc ggcgacttag tcgacctagg
     1081 cgatgcagtt ctcgtctcct gcaagatggt caagaccgag acgggcgagg tcgcctggca
     1141 ggacaagtac ctcggcccgc tcgcggacta cgacggcatc agtaagagcc tcgccgcgtc
     1201 cgccctgcgc gggctgggcg ccacgaccgc ggtcgcagta gtatcgcgac cctcgccgca
     1261 gctcacggtg gtcccgcctg aaaagaagga acaagccctg ctcgcgttct ccggcgccgt
     1321 ggaagccctg gaccaaaacg ataccgaggc ggccaaggtc cagctcgccg tggcccgggc
     1381 cattgacccc gccaacgcgg ccgtcacagc ctacctagcc aagctcgtca ccaacacgac
     1441 caagttcaag atacagctcg agtcctacgt gtcctatctt aatccggcct tcctcggcat
     1501 catccggcag gattccattc acctcgtgac cggtatgcca gtctggcccg tctattatat
     1561 gcccctcgtc gaaggactca actacacgag ctacggcgac gatcgctacg tctctgagac
     1621 ggtaagtgcc tcgcagttcg gctacagcct tcccctgggc gacgccttcg gtgtgcgcct
     1681 atcgggccat ttcgcggccc tcgacgtacg gaccagagtg ggatccccgc cgataagcga
     1741 tggtagcggc ggtcaatgga gcaataacaa ttccgcgcga ggcgccatcg gcggcggcct
     1801 ggacgcgggc gtacgcctgg gagacgcctt cgccataggc gcgggcttcg cgtacttcgc
     1861 ccgctcagac caggatacga gccccatagg acccatcgtc aagaacggcg gcacgacctg
     1921 ggcagccgat ctcggcttcc tcgtccgcaa tgccgacgag agcctggtat gggacacgcg
     1981 tgcgagctat atagcggaaa cacaagacct agtcgatccc ctgacgctcg ccctgaccgg
     2041 ggagacccag cccttcccct tgggcataga gaataacgtg accttctcac tgctcgggcg
     2101 caagcttttt ataatactaa aaaactcaga gtctatagcg ctcggcgata ccatgatctc
     2161 gagttccctc atgccggccg ccgagtattt cctcctgcca tggctttccc tccgctgcgg
     2221 tgtggagggt accatgctcg tcatggacgg cctcgagccg cagttcggcc tcggcggcat
     2281 gggaacgcag ctgcggcaag acactctgcg cctgggcctt gtcaatgtcg atgccgatct
     2341 ggcgatagcc ccagcgcacg gcatcggtgc cggaatgctt accgagcacc aactc
//
LOCUS       DAOOLN010000178         2394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_54923_length_2394_cov_4.021377, whole genome shotgun sequence.
ACCESSION   DAOOLN010000178 DAOOLN010000000
VERSION     DAOOLN010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54923_length_2394_cov_4.021377"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PLW80_03120"
     CDS             <1..391
                     /locus_tag="PLW80_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65524.1"
                     /translation="MIGRQSGDLYRSIYVSKVKSKKHVYRVGSKAVKSKQDFGGVAIV
                     RTTDATRLANIYEHAGGYTIQPKTKKALMFVTPDGQIVFTKRVSGQARPFMSASERAF
                     NWAQAFSAATDKVVIDEFAKLGIKVTP"
     gene            388..846
                     /locus_tag="PLW80_03125"
     CDS             388..846
                     /locus_tag="PLW80_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65525.1"
                     /translation="MTKSTIERMVVGLKTWLATNYPSYVTRANTGTTGVQAPAIRAFD
                     VSDVLTPGRFPQLIVSAGPVSIEATGPQVQMVTVACNVDIGVQAPNPGARETMLARYM
                     DALVDAIGENETIGGLVLSSSLDELDKDAIPADGRGFVVATIRLSDEVTT"
     gene            858..2162
                     /locus_tag="PLW80_03130"
     CDS             858..2162
                     /locus_tag="PLW80_03130"
                     /inference="COORDINATES: protein motif:HMM:NF039819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HPB65526.1"
                     /translation="MPSTNAVKITAGKETTVGTPATRTAVLPISEPGSLDRKIESAAD
                     PIIIGRNMLAGEYAVRGNVGGGVPITPRACAGMGLILKSALGGELASPPQAIGAIRIK
                     YTGAAASCKLELNGTADTITSKIGALGAETVDSAFGTAGVISLAATANDTIAELVALI
                     DSYADYSCELVFGTGTETIDSVITTASMQAKGKDAFIVLTGTSGVYAHRFQPDETYTG
                     GEPTPRPTLTVQLDGYGDNYVQAGHVVDQLSLSGALKDFLKGDVTFMGMTETGGASAS
                     ALTMEDAAPLNFADALTVIDGADYAYVRDVSLKIANGHRDDGYAQGSIDRAYHQRGTF
                     SCDGELTIRLDATALAIRAKVLSGTRGSLLLVFKGKALGSAGAYECMVIEVPYARFSS
                     ADRSANSGQFDMKVAWKALSPLGTQYDAPLTVWLFTDDSAVY"
     gene            2162..>2394
                     /locus_tag="PLW80_03135"
     CDS             2162..>2394
                     /locus_tag="PLW80_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65527.1"
                     /translation="MAKVNWKTASNRRVIGDWVEVKSLEGIGARVKPRKYSQAGADEI
                     NAWAVQRQAKMKRESVRALVDKKEDANLRDDAVL"
BASE COUNT          454 a          761 c          812 g          367 t
ORIGIN      
        1 gatgataggc cggcagtccg gcgacctcta ccggtcgatc tacgtgtcga aggtgaagtc
       61 gaagaagcat gtctaccgcg tcggctcgaa ggcggtcaag tcgaagcagg atttcggcgg
      121 cgtggccatc gtgcgaacaa ccgacgcgac gaggctcgcg aatatctacg aacacgccgg
      181 cggctacacg atccagccga agaccaagaa ggcgctcatg ttcgtcacgc cagacgggca
      241 gatcgtattc acgaagcgcg tgtccggtca ggcaaggccg ttcatgtcgg cgagtgagcg
      301 cgcgttcaac tgggcgcagg cgttctctgc ggcgaccgac aaggtcgtga tcgacgagtt
      361 cgccaagctc gggatcaagg tgacaccatg acgaaatcta cgatcgagcg gatggtcgtc
      421 gggctcaaga cctggctcgc gacgaactac ccgagctacg tgacgcgggc caacacggga
      481 accacgggag tgcaggcacc ggccatacgg gccttcgacg tgtctgacgt gcttacccct
      541 ggccgtttcc cgcagctcat tgttagcgcg gggccggtgt ctatcgaggc gaccggccct
      601 caggtgcaga tggtaaccgt ggcatgcaac gtggacatcg gcgtccaggc tccgaacccc
      661 ggggctaggg agacgatgct cgctcgctat atggatgcgc tcgtcgacgc catcggcgag
      721 aacgagacga tcggagggct ggtgctgtcg tcctcgctcg acgagctcga caaggatgcg
      781 atcccggcag acgggcgcgg cttcgtggtc gcgaccattc ggctgtcgga tgaagttacg
      841 acgtaaggag gcttggaatg cctagcacga atgcagtcaa gatcaccgcg gggaaagaga
      901 ccacggtagg cacgccggcc acgaggacgg cagtgctccc catttcggag cccggatcgc
      961 tcgaccgcaa gatcgagagc gcggcggatc cgatcatcat cggccggaac atgctcgcgg
     1021 gagagtacgc tgtgcgcggc aacgtgggcg gcggcgttcc catcaccccg cgcgcctgcg
     1081 ccggcatggg cctgattctc aagtcggcgc tcggcggaga gctagcctcg ccgccgcagg
     1141 cgatcggcgc gatacggatc aagtacacgg gagcggcggc gtcctgcaag ctcgagctca
     1201 acggaacggc ggatacgatc acgtcgaaga tcggcgcgct cggagcggag accgtggatt
     1261 cggcgttcgg caccgccggc gtcatctctc tggcggcgac cgccaatgac acgatcgcgg
     1321 agctcgtggc gttgatcgac agctacgccg actactcctg cgagctggta ttcggtaccg
     1381 gcaccgagac tatcgattcg gtcattacca ccgcgagcat gcaggccaag ggcaaggacg
     1441 ccttcatcgt gctgaccggg acgtccggcg tgtacgcgca ccgcttccag cccgacgaga
     1501 cctacaccgg cggcgagccg acaccgcgcc cgaccctgac tgtgcagctc gacggatacg
     1561 gcgataacta cgtccaggcc gggcacgtcg tcgaccagct gtcgctatcg ggggcgctca
     1621 aggatttcct caagggcgac gtcaccttca tgggcatgac cgagacgggc ggagcctccg
     1681 cgagcgcgct gaccatggag gacgcggcgc ccctgaactt cgccgacgcg ctcaccgtca
     1741 tcgacggcgc cgactacgcg tacgtccgcg acgtgtcgct caagatcgcc aacggccacc
     1801 gcgacgacgg ctacgcccag ggctccatcg acagggcata ccaccagcgc gggacgttct
     1861 cgtgcgacgg cgagctcacg atccggctgg acgccacggc gctggctatc cgcgcgaagg
     1921 tcctgtcggg cacccgcggc tcgctgctcc tcgtgttcaa gggcaaggct ctcggctccg
     1981 ccggcgccta cgagtgcatg gtcatcgagg tgccgtacgc caggttctcg tccgcggaca
     2041 ggtcggcgaa ctccggccag ttcgacatga aggtggcctg gaaggcgctc tcgccgctcg
     2101 gcacgcagta cgacgcgccc ctgacggtgt ggctcttcac cgacgactcg gcggtgtact
     2161 gatggccaag gtcaactgga agaccgcgtc gaaccgccgc gtgatcggcg actgggtcga
     2221 ggtcaagagc ctcgaaggga tcggggctcg cgtcaagccg cggaagtata gccaggccgg
     2281 cgcggacgag atcaacgcgt gggcggttca gcgtcaggca aagatgaagc gcgagtcggt
     2341 ccgggcgctc gtggacaaga aggaggacgc caacctccgc gacgacgcgg tgct
//
LOCUS       DAOOLN010000179         2393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_54960_length_2393_cov_3.288281, whole genome shotgun sequence.
ACCESSION   DAOOLN010000179 DAOOLN010000000
VERSION     DAOOLN010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54960_length_2393_cov_3.288281"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..198)
                     /locus_tag="PLW80_03140"
     CDS             complement(40..198)
                     /locus_tag="PLW80_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65528.1"
                     /translation="MRMRSALAKYEEAIVGIYNPYVTTITFEETPVGANEMGERIVKN
                     GGRLLARF"
     gene            complement(482..2239)
                     /locus_tag="PLW80_03145"
     CDS             complement(482..2239)
                     /locus_tag="PLW80_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65529.1"
                     /translation="MDKDFGSRDDKIDAIITSSGYPLAVKSLLQRLDESDVIASVSLG
                     SGRDAVLLAAVAARLQAEPSLRALVIAPSDREVAGLERTFEGVAGPGGPSSAAIGREA
                     SMEDARASIGSLDAIASRIAAGTLDPDDFALIALSDLDPFAGAASVAMLRHALGSGST
                     ERRLIGFAEELRPAHRAVVAPLCDSLVEIELEDEGQRVKDAPASSFTATAEDKPRLLL
                     GLLARDEDRPFVVFCNLKDSAESVARMLRSRGVKTEYVLGNLQRKRAVLDSVVAGEYE
                     ALVLTDEGAAGLPGAWAARLVNWDLPLEGEPYLSRLVHLDASARGARVYNFACDRYSL
                     GIPAIERALGRRLEPTRADESIMSFAGPVEPTAPEPRARAAETPRPDRRKPEPRRSEV
                     REHRGQQDRRDRVGQYDGRNIKAIQDDIAAITGGKPSATPNRARAQLPAGKGVEQPRR
                     GKKARGTADKTPRQDTTAARREAPDRAGKGVTTPAGRRSRGGSESRIRDPYSISMEER
                     LRLYRERYGSAGEPKPAGGKPSAAQPSRSSQERKRSEPRTAPVQRAPAVESVPVPPPD
                     PMPRGILGAIKGLFGKKDE"
BASE COUNT          354 a          795 c          832 g          412 t
ORIGIN      
        1 ccagaccggg gcctgtgagc tggccagggg gctacagacc tagaaccggg cgaggagccg
       61 cccgccgttc ttgactatgc gttcccccat ctcgttcgcg cctaccggcg tctcctcgaa
      121 ggtgatcgtc gttacgtacg gattgtagat cccgactatc gcttcctcgt acttggccag
      181 ggcgcttcgc atacgcacgg ttcaattctc tcgtcagcga tcagaagaac gcggcccagg
      241 cgaggccgga cagtagtgcc gccgctacgg ccgagccgag gtatacggcg aagacgcgcg
      301 gtttggttat gccgaatacg gccgacatcg ccggcagcgt cgtcgtcgcc cctccaagca
      361 ggaacgcaag ggcggctgcc tgaggcgaag cgcaacgaag agccgagctc ccggtaacgg
      421 gagccctcga ggccagccag gcgatctaag gcttggtcgg atagcgtagc gtcgggcttg
      481 cctactcgtc cttcttgccg aagagtccct tgatggcgcc caggataccc ctgggcatgg
      541 gatccggcgg gggcacgggt acggactcca cggctggcgc ccgttggacg ggggccgtgc
      601 ggggttcgga tcgcttgcgt tcctggctgg accgggaagg ctgcgcggcc gaaggcttgc
      661 ctccggcggg cttcggctct ccggctgaac cgtagcgctc gcggtacagg cgtagccgtt
      721 cctccatgct tatcgaatag ggatcgcgta tgcggctttc ggaaccgccg cgcgaccgcc
      781 tgcccgccgg tgtcgtaacg cccttaccgg ccctgtccgg agcttcccgg cgggcggccg
      841 tcgtgtcctg cctgggcgtc ttatcggccg tgccccgagc cttcttgccg cgtctcggtt
      901 gctcgacgcc cttgccggcc ggcagctgcg cccgcgctcg gttcggcgtc gcggacggct
      961 tgcctccggt gatggcggcg atgtcgtcct ggatcgcctt gatgttccgg ccgtcgtact
     1021 ggccgacgcg gtccctgcga tcctgctgcc cgcggtgctc tcgcacctct gagcgacgcg
     1081 gctcgggctt ccggcgatcg gggcggggcg tctcggcggc acgagcgcgc ggctcggggg
     1141 ccgtaggctc gacgggtccg gcgaaggaca tgatggactc gtcagcacgg gtcggttcga
     1201 ggcgacggcc cagggcccgc tcgatggccg ggatgccgag cgagtaccga tcgcaggcga
     1261 agttatagac gcgggcgccg cgcgccgacg cgtccagatg gacgaggcgt gagaggtatg
     1321 gctcgccctc gagcggcagg tcccagttga ccaggcgcgc ggcccaggcg cccggcaggc
     1381 cggcggctcc ctcgtcggtc agcacgagcg cctcgtattc cccggctacg accgagtcga
     1441 gcacggcgcg tttccgttgc aggttgccga gcacgtactc ggtcttgacg ccgcgcgatc
     1501 gcagcatgcg cgccaccgac tcggcgctat ccttgaggtt gcagaagacg acgaacggcc
     1561 gatcctcgtc gcgcgcgagc aggccgagca gcaggcgcgg cttgtcctcg gccgtggccg
     1621 tgaagctcga ggcgggagcg tccttgacgc gctggccctc gtcctccagc tcgatctcga
     1681 cgagcgagtc gcagagcggg gcgacgaccg cccgatgcgc cggcctgagc tcctccgcga
     1741 agccgatgag gcgccgctcc gtcgagcccg agccgagcgc atgcctgagc atcgccacgc
     1801 tcgcggcgcc ggcgaacgga tcgaggtcgc tcagggctat cagcgcgaag tcgtcgggat
     1861 cgagggtgcc ggcggctatc ctcgaggcta tggcgtcgag cgagccgatc gaggccctcg
     1921 cgtcctccat agaggcttcg cggcctatcg ccgcggagga agggccgcca ggcccggcga
     1981 cgccttcgaa ggtccgctcg aggccggcga cctcgcggtc ggacggggca atgaccagcg
     2041 ccctgaggct cggctcggct tggagcctgg cggctacggc ggcgagtaac acggcgtctc
     2101 gccccgagcc gaggctgacg ctggcgatga cgtcgctctc atcgagcctc tggagcaggc
     2161 tcttgaccgc cagcggatat cccgacgacg tgatgatcgc gtcgatctta tcgtctcttg
     2221 aaccgaagtc tttatccatg attatcccgt ggctattcgt ttctcagcgc gcctgatacg
     2281 gcgcacgtat actgattata caatacaatg ccataaaaac atctggaagg ctaggcgatc
     2341 gccgctctcg agttgcccgc gagggcgatt atcgtataga gtcgagcgat gat
//
LOCUS       DAOOLN010000180         2390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_55049_length_2390_cov_3.150749, whole genome shotgun sequence.
ACCESSION   DAOOLN010000180 DAOOLN010000000
VERSION     DAOOLN010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55049_length_2390_cov_3.150749"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(884..1429)
                     /locus_tag="PLW80_03150"
     CDS             complement(884..1429)
                     /locus_tag="PLW80_03150"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPB65530.1"
                     /translation="MKHLSRRKKAPFLRTILIAAVALPIPAFAASPLPPLVEAVVKND
                     AKRTAALIREEADVNVFQEGMDTPLVEAVDREPSKFNLEILKLLLGAKDIQVNKYSAL
                     SMGSYVWERTPLIAAAYNGNAEAVKLLLAKGAKVDLTDTYYPISDVLGPEPRSTALTW
                     AATRGHYDVVVLLVEAGARIE"
     gene            complement(1754..>2390)
                     /locus_tag="PLW80_03155"
     CDS             complement(1754..>2390)
                     /locus_tag="PLW80_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPB65531.1"
                     /translation="FGGDGWAYDIGFGGLDHVIASGRNVNIMVLDTEVYSNTGGQMSK
                     ATPFGAVAKFAAAGKDIGKKDLPMIAMSYGYVYVAHIAMGANMNQALKAIREAESYDG
                     PSIVVAYSHCAMHGIDMMKGMDQQKLVVESGIWPLFRYDPRLVAQGKNPFQLDSKEPQ
                     LDKVSTFMGNEIRFRTLRAADAERADMLEAKEKALVERRWREYKYLADRPF"
BASE COUNT          438 a          709 c          770 g          473 t
ORIGIN      
        1 atagattccg aggcgacgac tcaggcgtga taaccgccgt ttttccggcc acattgtcat
       61 ttgcattaac tatgcttacc attccggccg gcagcttcag ccgttacggt acgaccacga
      121 acgtcagcgt cataactcga ccctaccgca taagcgccta cgagataacc cggacccagt
      181 tcgtaagctg gtaccgcgcc atcgccttct gaaacaagct gagcctggcg gaggggttgg
      241 agcccgtgta cgcggtagac ggagtggact ttattacgct taccttcggt cagattcctg
      301 gaagctcaaa tgacgcatgg accaatgcga ccgctacatg gaccaacgac ggctatcgcc
      361 tacccacgga gatggaatgg atgtgggctg cgatgggcgt tcccgcggac gggcgggacg
      421 gaggcgtcaa cgcgacgggg tacgctaagg ccttcgccgg cagcacggga agcaatagcc
      481 tggacgatta cgcgtggcac ttcgataaca ataatggcag caatacgacg catccggtag
      541 gagcgaagct ccccaatgag ctcagcggac ccgtttcggg taccttacgg gtaaggcgcg
      601 ggggtagctt tgataataag aatacggaga acttgagcgt tgcttacagg gttgaagctt
      661 aaatgtattc cggttcatta gtcgccgggt tccgggtcgt tcgtaattag gttgcgctgt
      721 ctatagatag tcttagcgga cgagcgctat cgcgaagtcc cggctatgaa gggggggccg
      781 tgctcaggca cggcccttct ttgggcctct ggtgaaaacc tggtttcctt gcccatgact
      841 gcatcctctc aaagaaatca gtctccggta atcctggggc ggttcattct atccgggcgc
      901 cggcctcgac gaggaggacg acgacatcgt agtggccgcg cgtggccgcc caggtcaggg
      961 cggtggaacg tggctcgggc cccaggacgt cgcttatggg atagtaggtg tcggtcaggt
     1021 cgaccttggc gcccttggcg aggagcagct tgacggcctc cgcgttgccg ttgtaggcgg
     1081 ccgcgatgag cggcgtccgc tcccagacgt agctacccat ggagagcgcg ctgtacttgt
     1141 tgacctggat gtccttggcg cccaagagca gcttcaggat ctccaggttg aatttactcg
     1201 gttcccggtc gacggcctcc acgagcggcg tatccatgcc ttcctggaag acgttgacgt
     1261 cggcctcctc gcgtatgagg gcggcggtcc tcttggcgtc gttcttgacg accgcctcga
     1321 cgaggggcgg tagcggcgac gccgcgaagg ccggtatggg cagggcgacg gccgcgatca
     1381 gtatcgttcg aaggaatggc gccttcttcc gcctggacag atgcttcaaa ctcgacgctc
     1441 ctgggcttgg catgagatct cctgatcgat gggacatatc cgaaccacta cttgaatgta
     1501 tgggcatgcg ccgagcgata gcaagcgccg acgcctaccg tacgagcccg aaacccgacg
     1561 ttccaaggca tgcggtctcg aggaaccgtt acgggccgaa aacaaaagcc cgccggcgcg
     1621 cgagcgccga cgggcttccg taatacagct cagtccaaag cttaacccgc gcgcgcagcg
     1681 cgtcgggtta aagcgggcgg gagcggtatc gcggctccgc taggagtccg cgataccctc
     1741 gaccgaccgt tcgtcagaac gggcggtcgg ccaagtattt gtactctctc cagcgcctct
     1801 ccacgagcgc cttctccttg gcctcgagca tgtcggcgcg ctcggcgtcg gccgcccgca
     1861 gggtgcggaa gcggatctcg ttgcccatga aggtggacac cttgtcgagc tgcggctcct
     1921 tggagtcgag ctggaagggg ttcttgccct gggcgacgag gcgcgggtcg tagcggaaca
     1981 gcggccagat gcccgactcg acgacgagct tctgctggtc catgcccttc atcatgtcga
     2041 tgccgtgcat ggcgcagtgg ctgtaggcga cgacgatgga cgggccgtcg tagctttccg
     2101 cctcgcggat ggccttgagc gcctggttca tgttggcgcc catcgcgatg tgggccacgt
     2161 acacgtagcc gtagctcatc gcgatcatcg gcaggtcctt cttgccgatg tccttgccgg
     2221 ccgcggcgaa cttggccacc gcgccgaagg gcgtggcctt ggacatctgc ccgccggtgt
     2281 tcgagtacac ctcggtgtcg agcaccatga tgttcacgtt gcggccggag gcgatcacgt
     2341 ggtccaggcc gccgaagccg atgtcgtagg cccagccgtc gccgccgaag
//
LOCUS       DAOOLN010000181         2390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_55057_length_2390_cov_2.638972, whole genome shotgun sequence.
ACCESSION   DAOOLN010000181 DAOOLN010000000
VERSION     DAOOLN010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55057_length_2390_cov_2.638972"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="PLW80_03160"
     CDS             complement(<1..1056)
                     /locus_tag="PLW80_03160"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPB65532.1"
                     /translation="MKYLVLAIAVVMYGLVIAFPHKKAWFTLGAALAVAALGVVSPAV
                     IFGELINWNVLLIYVGSLVIADLFIYSRVPSRLADRIVARAPNLGWGVVFILIMTGLL
                     SAFVENVATVLVMAPIALALSDKMKVDARYFMMGLAVMSNLQGTATLVGDPPSMVFAG
                     YSFNDFFFKDGKPGVFFAVQVGMLVGAAFFYYYYRGMKNEKVDLPDERVKSWFPTLLL
                     AALVVGLAVISFLFEGFSILSGGFVFALGLVGLAWYVGVMKESRAQAWRLVKSLDWET
                     ILFLIGIFVVVGAVSETGLLEDLSSLLASLVGDSVLTGFVLIILMSVLLSGFIDNVPY
                     IIVMLPVAAKLAAGLGLR"
     gene            1354..>2390
                     /locus_tag="PLW80_03165"
     CDS             1354..>2390
                     /locus_tag="PLW80_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65533.1"
                     /translation="MCDCFYLPTSRGAFFGKNSDRHATEPQALRLVPRRRPSPSVSFG
                     GKSFELPDAGHALALSCPCWMPGAEMGLNGAGVAIGNEAVFSRFKAAPDGVLGMDFVR
                     AALSASSSAHEALDSLISLTERYSQGGNGAYKGRLVYSNSYLVVGPDGAYVLETAARR
                     WAWKEVSGPTALSNSYSITDDYKRLDAETRKAIAPVNERMACLDEADAGRIGEKGSWK
                     AYVENRFMSRFSAGDARRRALGSLVTAIAETGDRRAAMAALRAHSAIDPDRPSRPRNV
                     CDHDLDIMGNPTTGSMLVEYREDATVLWFTGAPYACANLFKPVLLSNGEFVPLWTDYD
                     YVEGSGGGEAY"
BASE COUNT          461 a          743 c          776 g          410 t
ORIGIN      
        1 gcgcaggccg agcccggctg cgagtttggc cgcgacgggc agcatcacga tgatataggg
       61 tacgttgtcg atgaagcctg agagcagcac cgacatgagt atgataagca cgaagccggt
      121 gagcacgctg tcgccgacga ggctggctag cagggacgac aggtcctcga gcaggccggt
      181 ctccgacacg gccccgacga cgacgaagat gccgatcagg aagagtatcg tctcccagtc
      241 gagcgacttg acgaggcgcc aagcctgggc gcggctctcc ttcatcacgc cgacgtacca
      301 cgcgagtccg acgaggccga gcgcgaagac gaagccgccc gacagtatgg agaagccctc
      361 gaacaggaac gatatgacgg caaggccgac gacgagcgcc gcgagcagaa gagtaggaaa
      421 ccaggacttg acgcgctcgt ccggcagatc gaccttctcg ttcttcatgc cgcggtagta
      481 gtaatagaag aacgccgccc ctacgagcat gcctacctgt accgcgaaga acacgccggg
      541 cttgccgtcc ttgaagaaga agtcgttgaa cgagtatccg gcgaatacca tcgatggcgg
      601 gtcgcctacc agcgtcgcgg tgccctggag gttcgacatg accgcgagtc ccatcatgaa
      661 gtatcgcgcg tccactttca tcttgtcgct gagcgccagc gctattggcg ccatcacgag
      721 cacggtggcg acgttctcga cgaaggcgga caggaggccc gtcatgatca ggatgaacac
      781 gacgccccag cccaggttcg gcgccctggc tactatcctg tcagcgagcc gagacggcac
      841 gcgcgagtag atgaagaggt cggcgatgac gaggctaccc acgtagatga gcaggacatt
      901 ccagttgatc agctcgccga agatgacggc gggcgatacg acgccgagcg cggcgaccgc
      961 tagggccgcg ccgagcgtga accatgcctt cttgtgcggg aaggctatca cgaggccgta
     1021 catgacgacg gcgatagcca gtaccagata cttcatgcga ttactccttc gcggcgggct
     1081 gtcgcgctaa aaaaagacct ttcgcatggg tccgtaaacg ggcgcatgcg aaaggtcttg
     1141 tttccgacgg cgcctcggta aggctacggc ggccgtccga ccaggctgac ccgtttctgg
     1201 gcgccggttg ttcatgcgga cgatgcaact actcccctct cgatacgccc gtgaggatac
     1261 gtatatcgga gccggaggtc aagccgcgcc ggccgctacg gatttgtcat ggcggcgttc
     1321 cgcgattata gtactatgcg agcggaggga accatgtgtg actgtttcta ccttcccacc
     1381 tcgcggggag cgttcttcgg taagaattcc gaccgtcatg ccaccgagcc tcaggccctg
     1441 cggctcgtgc ctaggcgtag accgtcgccg tccgtctctt tcggcggcaa gtcgttcgag
     1501 cttccggacg ccggtcacgc gctcgctctg tcctgcccgt gttggatgcc cggcgccgag
     1561 atgggcctga acggcgccgg ggtggctatc ggcaacgagg ccgtgttcag ccgcttcaag
     1621 gccgcgcctg acggcgtcct gggcatggac ttcgtgcgcg cggcgctgtc ggcgtcgtcg
     1681 agcgcccatg aggcgctcga ctccctgata tcgctgacgg agcgctacag ccagggaggt
     1741 aacggtgcct acaagggtcg gcttgtctac agcaactcat acctcgtcgt aggccccgac
     1801 ggggcctacg tccttgagac cgcggccagg cgctgggcct ggaaggaggt ctccggcccg
     1861 acggccctat ctaattccta ttcgataacg gatgattata agcgcctcga cgccgagacg
     1921 cgcaaggcca tagccccagt caacgagcgg atggcctgcc tcgacgaggc ggacgccggg
     1981 cggataggcg agaagggttc ctggaaggcc tacgtcgaga accgcttcat gagccgcttc
     2041 tcggccggag acgcgaggcg ccgggcgctc ggctcgctgg ttacggcgat cgccgagacc
     2101 ggcgatagga gagccgctat ggccgcgctc agggcgcact cggccatcga cccggatcgc
     2161 ccctccaggc cccgcaacgt ttgcgaccat gacttggata tcatgggcaa cccgacgacc
     2221 ggctcgatgc tggtggagta tcgcgaagac gcgaccgtac tgtggttcac gggcgctccg
     2281 tatgcctgcg ccaacctgtt caagcctgtg ctcctgtcca acggcgaatt cgttccactc
     2341 tggaccgatt acgactatgt cgagggctcg ggcggcgggg aggcctactg
//
LOCUS       DAOOLN010000182         2388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_55126_length_2388_cov_2.642949, whole genome shotgun sequence.
ACCESSION   DAOOLN010000182 DAOOLN010000000
VERSION     DAOOLN010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55126_length_2388_cov_2.642949"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..536)
                     /locus_tag="PLW80_03170"
     CDS             complement(18..536)
                     /locus_tag="PLW80_03170"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPB65534.1"
                     /translation="MLATLATLAAARPALAVSLSPPRATSQPRPLESELSEPSEPIRS
                     AASDPRDDRPKVAVDTTVDTTAGEVAAGLAGPPVDAPEPGAAESAEADPGAVDVEAAG
                     EAAREAAVMARIQAAKAYPDAARRRGIEGEVVVAFAIAADGSLLRVDAEPGPVHVLLE
                     KDMNGPGLGVHP"
     regulatory      complement(859..1058)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1177..2292
                     /locus_tag="PLW80_03175"
     CDS             1177..2292
                     /locus_tag="PLW80_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPB65535.1"
                     /translation="MIDARLEKLAKILVNYSVKVKPGENVLVQDTGIEPAFDRALVKA
                     IHEAGGRAFVSLRDKTLDRALLRDAPPEQVELQARFERERMDAMDCFIGFNAWRNLSS
                     WSDLSGDELDLYNKNIWKKVHIESRLPGTRWVVLRFPTAAMAQMAAMSEEAFEDFFFD
                     VCTMDYARMSKAMDALVSYLAKTDEVRIVGPGTDLSFSVKGLPPIKCDGTMNIPDGEV
                     YTAPVKGSVNGVIQYNTPSEHEGFTYENIRFEFKDGKIVKATSNDNERINRVLDTDED
                     ARYVGEFAFGVNPYITRAMKETLFDEKISGSIHFTPGNSYDDCFNGNRSAVHWDLVLI
                     QTPEFGGGEIWLDGRLVRKDGLFVVPELEGLNPKNLM"
BASE COUNT          383 a          809 c          817 g          379 t
ORIGIN      
        1 ccgccgacgg gtcgcttcta cgggtggacg ccgagcccgg gcccgttcat gtccttctcg
       61 agcaggacat gaacgggccc gggctcggcg tccacccgta gtagcgaccc gtcggcggct
      121 atggcgaacg ccacgaccac ctcgccctcg atgcccctcc gcctggcggc gtccgggtag
      181 gccttggcgg cctggatgcg agccatcacg gcggcctctc tggcggcctc cccggcggcc
      241 tcgacgtcga cggcaccggg atcggcctcg gcgctttcgg ccgccccggg ctcgggcgcg
      301 tcgaccggcg gtcctgcgag ccccgcggcc acctccccgg ccgtcgtgtc gaccgtcgtg
      361 tcgaccgcga ccttgggccg atcgtcccgg ggatcgctcg ccgccgaacg gatcggttcg
      421 gatggctcgg atagttcgga ctcgagcggg cgcggctgag aggttgcgcg cggcggcgat
      481 agcgatacgg cgagcgccgg ccgtgccgcg gccagcgtgg ccagcgtggc cagcatggcg
      541 ctggtcggcc gaggccgccc gatcaggctg gccgctaacg ccagggctgc tacgtggatc
      601 gcgaccgagg cgatcgcggc gcccgcccgg cgaaccgatc gccggcgtcg cgggacgtcg
      661 gtcgattgtg gcgaggggcg ccagtcctgc ctcgttccat gcctcatccg cgatacctcg
      721 tatggagcgg cgggggaggt cgagcggcgg cgttacgctg ggagggacgg gccccggaga
      781 acgagggcgg acggctcccg atgcgggggc ctcaaggagg ctacccgccg gcgtatcgct
      841 agcggctcac ctcgtaccgc gcgaaaggat cgccttggca ggtcttctgg cttccgggtc
      901 gtccggcggt cgcgccttcc cggccccgga agggccagtg gcgatgagcg atcgtcgtat
      961 ccgggtacag cggcgggtcc gctcccgatt tgaacgggat tccctcttgg cggcgggcag
     1021 cttgggccgc tcgccgaacc ttggcgtgtc ctcgttatac ggactaacgc ctcggaggtc
     1081 aagcgacgcc cggccccgtt tctagtcgcc ggaacggggg cggtactttc tggctatgct
     1141 ctctcccgtg ctatactcgc ccgcgaggag aaccccatga tagacgcaag gctcgagaag
     1201 ctggccaaga tactcgtcaa ttactcggtc aaggtaaagc cgggagagaa cgtgctcgtg
     1261 caggataccg gcatcgagcc ggccttcgac cgcgccctgg tcaaggcgat tcacgaggcc
     1321 ggcggccgcg ccttcgtgtc gctacgcgac aagacgctcg accgcgccct gctccgtgac
     1381 gcgccgccag agcaggtgga gctccaggcc cgcttcgaac gggagcggat ggacgcgatg
     1441 gactgcttca tcgggttcaa tgcctggcgc aacctctcct cgtggtcgga cctatccggc
     1501 gacgagctcg acctgtacaa caagaacatc tggaagaagg tgcacatcga gtcgcgcctg
     1561 ccgggcaccc gctgggtggt gctacgcttc ccgaccgccg cgatggccca gatggccgcg
     1621 atgagcgagg aagccttcga ggacttcttc ttcgacgtgt gcacgatgga ctacgcccgc
     1681 atgtccaagg cgatggacgc cctggtgtcc tacctggcta agaccgacga ggtacgcatc
     1741 gtcggcccgg gcaccgacct ctccttctca gtcaagggcc tgcccccgat caagtgcgac
     1801 ggcacgatga acattcccga cggggaggtc tacaccgctc cggtcaaggg ctcggtcaac
     1861 ggcgtcatcc agtacaacac gccgagcgag cacgagggct tcacctacga gaacatccgc
     1921 ttcgagttca aggacggcaa gatcgtcaag gcgacgagca acgataacga gcgtatcaat
     1981 cgggtgctgg acaccgacga ggacgcccgc tacgtcggcg agttcgcgtt cggcgtcaac
     2041 ccgtacatca cccgagcgat gaaggagacc ctgttcgacg agaagatctc cgggtccatc
     2101 cacttcacgc cgggcaacag ctacgacgac tgcttcaacg gcaaccgctc ggccgtccac
     2161 tgggacctgg tcctgatcca gacgcccgag ttcggtggcg gagagatctg gctcgacggc
     2221 aggttggtac gcaaggacgg cctcttcgtc gtgccggagc tcgaggggct caacccgaag
     2281 aacctcatgt gacgacggct tgagtgggcg ttccgccgca tggtaccctg gcgcgcatga
     2341 atcgaacgct ttcaggcgcc ggggccgccc ggacggccgc cgcggccg
//
LOCUS       DAOOLN010000183         2387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_55150_length_2387_cov_3.396226, whole genome shotgun sequence.
ACCESSION   DAOOLN010000183 DAOOLN010000000
VERSION     DAOOLN010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55150_length_2387_cov_3.396226"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..1273)
                     /locus_tag="PLW80_03180"
     CDS             complement(11..1273)
                     /locus_tag="PLW80_03180"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPB65536.1"
                     /translation="MSQQMLLIAMTLEFAVFALMLGLLASQLRGKVRGPLEWACHALL
                     SFAASATYILLGGRYSIPLLFLANALLYAGILAIPIGLAYYLGERPRWGWYVFGWALG
                     LAIFFWFMVLQPNTAARVWAYSLFGASVFMFSAGYILKRAPDDMAVPTRIAAAFYAIY
                     SLLNLGRIVVFILRSRQVPVFGRLVGDEVFFVGALFIYPGMVFAELQLVSARLVLDLA
                     KAVDEKALMAREMNHRIKNSLALAESLVELQRGEGDDTAFGEALASIRSRLHSISLVH
                     ARLYHEGADGSIRADEYLGALAADLCYNLGYTDLRTNLEPLSVDAAVAMPLGVITNEL
                     ITNAIKYGNAPGSRRLELSLSERGGAEAILRVADGGPGFSENDKPGLGTTLVDSLAAQ
                     LDGSVRRFTEGGAVVEVTIPLPGRRSSR"
     gene            complement(1278..>2387)
                     /locus_tag="PLW80_03185"
     CDS             complement(1278..>2387)
                     /locus_tag="PLW80_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB65537.1"
                     /translation="QELRTPLQGILGAIDLLGRTRLDASQSKSLAMARSSSEQLNAVL
                     TNILDAMGAERGTLRIEPAEFRYLEFASWLESELRPRAEDQGLGFRFSARGQDRLVNA
                     DRRRISQVIMNLCANAIGYTERGEVELSMALGDEALYVSVKDTGPGLTEEARKHLFQP
                     FYDASRIRKGAGIGLGLAQVKAIVDAMDGSIRYETSPSIGTRFEVSLPIQSVADDGAT
                     EGEAGRESVTAEAPALTSGGRVIVAEDEAINRLYLKRLLETSGYTVSPAGNGLDALEA
                     AGSGSWDFILMDVSMPRMDGLEATRRIRAMEADRGLTRTPIIALTAHAYAEDRQACVA
                     AGMDGFLSKPFAEPALWDEVRRVSATVALNRPGQG"
BASE COUNT          420 a          786 c          777 g          404 t
ORIGIN      
        1 gcgatagggc ttagcgactc gacctgcggc ccggtagcgg gatcgtcacc tcgacgaccg
       61 cgccgccctc ggtgaagcgc ctgaccgaac catccaattg ggcggccagc gagtcgacca
      121 gcgtcgtgcc gaggccgggc ttgtcgttct cggaaaatcc ggggccgccg tccgctacac
      181 ggaggatcgc ttcggccccg cctcgctcgg acagcgacag ctccaatcgc ctcgagcccg
      241 gcgcgttgcc gtacttgatg gcgttcgtga tcagttcgtt cgtgatgacg cccagcggca
      301 tggccacggc cgcgtcgacc gagagcggct cgaggttcgt cctcaggtcg gtgtagccga
      361 ggttgtagca taggtcggcc gcgagcgcac ctaagtactc gtcggcgcgt atcgagccgt
      421 ccgcgccctc gtggtatagc ctggcgtgaa cgagggaaat cgagtgcagc ctcgagcgga
      481 tcgaagcgag cgcctcgccg aaggccgtgt cgtcgccctc gccccgctgc agctcgacga
      541 ggctctcggc cagggccagg ctgttcttga tccggtggtt catctcccgc gccattaggg
      601 ccttctcgtc cacggccttg gccaagtcca gcaccagccg ggcgctgacg agctgtagct
      661 cggcgaagac catgccggga tagatgaaca gcgcgccgac gaagaagact tcgtcgccga
      721 cgagccggcc gaataccggt acctgtcgcg agcgtaggat gaagacgact atgcggccca
      781 ggttcaggag cgagtagatc gcatagaacg ccgccgctat cctcgtcgga acagccatgt
      841 catcgggagc ccgtttcagt atatagccgg cgctgaacat gaagacgctc gccccgaata
      901 gggaataggc ccagacgcgc gcggccgtat tgggctggag aaccatgaac cagaaaaaaa
      961 tcgccaagcc cagggcccag ccgaagacgt accagcccca tcgcggacgc tcgccgaggt
     1021 agtacgctag tccgatgggg atagctagta tgcctgcgta gagtagggcg ttggccagga
     1081 atagcagcgg aatcgaatac cggccgccaa gcaggatgta ggtggccgat gcggcgaacg
     1141 acaggagcgc gtggcaggcc cattcgagcg gcccgcgcac cttgcccctg agctgggacg
     1201 ccaacaggcc gagcatgagc gcgaacacgg cgaattcgag cgtcatggct atcaggagca
     1261 tctgctggga catgcggcta tccttgtccc gggcgattga gcgccacggt cgccgagacc
     1321 ctgcgcacct cgtcccagag cgctggttcg gcaaagggct tggacaggaa gccgtccatg
     1381 ccggcggcta cgcaggcctg cctgtcctcg gcgtaggcgt gggccgtcag cgcgatgatg
     1441 ggcgtccggg taaggcctcg gtccgcctcc atggcgcgga tgcggcgggt agcctcgagg
     1501 ccgtccatcc tgggcatgct cacgtccatc aggatgaagt cccacgaacc cgacccggcg
     1561 gcctcgagcg catccagtcc gttgccggca ggcgataccg tgtaaccgga cgtctcgagc
     1621 agccgcttca ggtagagcct attgatcgcc tcatcctcgg cgacgataac ccggcccccg
     1681 ctcgtgagcg cgggcgcctc ggcggtcact gattctctcc ccgcctcgcc ttcggtagcc
     1741 ccgtcgtcgg cgaccgactg tataggaagg ctcacctcga agcgggtgcc gatgctcggg
     1801 ctggtctcgt agcgtatcga gccgtccatg gcatcgacga tggccttgac ctgggccagc
     1861 ccaaggccga taccggcgcc cttccgtatc ctgctcgcgt cgtagaacgg ctggaacagg
     1921 tgcttcctgg cctcctcggt caggcctggg ccggtatcct tgaccgagac gtatagggcc
     1981 tcgtcgccga gcgccatcga taactcaacc tcaccccgct cggtatagcc gatggcgttg
     2041 gcgcagaggt tcatgatcac ctgcgatatg cgtcgccggt cggcgttgac gagccggtct
     2101 tggccacggg ccgagaacct gaacccgagg ccctggtcct ccgccctcgg gcgaagctcg
     2161 gactcgagcc atgaagcgaa ctccaggtac ctgaactccg cgggctctat ccgtagcgtt
     2221 ccccgctcgg cgcccatcgc gtcgaggata ttcgttagca ccgcgttgag ctgctccgag
     2281 gacgaacggg ccatagccaa cgatttcgac tggctcgcgt ccagccgggt cctacccagg
     2341 aggtcgatgg cgccgagtat tccctgcaag ggcgttcgta gctcctg
//
LOCUS       DAOOLN010000184         2385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_55228_length_2385_cov_1.995708, whole genome shotgun sequence.
ACCESSION   DAOOLN010000184 DAOOLN010000000
VERSION     DAOOLN010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55228_length_2385_cov_1.995708"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PLW80_03190"
     CDS             complement(<1..714)
                     /locus_tag="PLW80_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65538.1"
                     /translation="MSEEIDPEIAALIGGGIEEYSAKPAGRVPGAGGPSFEALFGDMG
                     VAGDVEAKGEFDVDLSKKAYDAVERFESDPPNAFFDDPQYYQKALAGEGEASQRFHEL
                     LKKYLQATDPKDRGMYRQQVITAYWNMVSRMIPRVVSANPIVPKQLMIRFGLSLPTMI
                     SAEQRAIFSRIIHQKTTSEPVYYLDEWLRSIAMGQVNPSSTDEVSQARGDERARINTL
                     LQKAMGKRDGHWKKLLGLVA"
     gene            complement(1113..1185)
                     /locus_tag="PLW80_03195"
     tRNA            complement(1113..1185)
                     /locus_tag="PLW80_03195"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1150..1152),aa:Ala,seq:ggc)
     gene            1336..2259
                     /locus_tag="PLW80_03200"
     CDS             1336..2259
                     /locus_tag="PLW80_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65539.1"
                     /translation="MGIEKVVAGELERLGLAASGRSAGRVGFEADKAGLAKALVGLRC
                     ADRVFLEAGRFRARDFDELYEGFKAIPWERWVGPQDRLVIEKARSIRSALSAQNAIQS
                     VGQKAAYERLCGEYGVGRMPETAATVQTRVRIERDEATVELDLCGEALSRRGYRKRPT
                     EAPLKESVAAAVHLLGADRIGHGVRAIEDPALLTILRDRQIPLEINPTSNICLHVYRR
                     IAEHPLPHLDRMGLKLTINSDDPPLFNTTLCDEYRLLAGEFGYDRAGIARLARNAFEV
                     NRAEPALKQRLLAEFDAWCLTDDLDLLVQTS"
BASE COUNT          432 a          743 c          748 g          462 t
ORIGIN      
        1 tgcaacgaga ccgaggagtt tcttccaatg cccatcgcgc ttgcccatcg ccttctggag
       61 caacgtattg atcctagccc tctcgtcgcc cctggcctgg ctcacctcgt cggtactgct
      121 cgggttgacc tggcccatcg cgatggagcg cagccactcg tcgagataat agaccggctc
      181 ggaggtcgtc ttctgatgga tgatcctgga aaagatggcc cgttgctccg cggatatcat
      241 cgtaggcaac gagaggccga aacggatcat gagctgcttt gggacgatgg ggttagctga
      301 tacgaccctc ggtatcatac gcgagaccat attccagtag gcggtgatca cctgctggcg
      361 atacatgcct cgatccttcg gatcggtggc ctgcaggtat ttcttgagta gctcgtggaa
      421 gcgctgcgac gcctcgccct cgccggcgag cgccttctgg taatactggg ggtcgtcgaa
      481 gaaggcgttt ggcggatccg actcgaagcg ctcgaccgcg tcgtaggcct tcttggacaa
      541 gtcgacgtcg aattcaccct tggcttccac gtcgccggct acgcccatgt ccccgaacag
      601 cgcctcgaag cttggcccgc cggccccggg cactcgtccc gccggcttgg ccgagtattc
      661 ctctattccg cctccgatga gcgccgcaat ctcaggatcg atctcttcag acatcgcgtg
      721 ccgtccccgc attatagatg gatgctacgt actcgcgtag tgtactccat cgacgcgccg
      781 cggacaaggt taggggtctg atccccccgc gagtactctc gcgcccctcc atacgggtta
      841 gtaaaagaaa aagaccgccg gtaggcggca taacagaggg attggtccta actcgccggg
      901 gtaccggcga gggaattgcc ctccattcga aaacatcctg ttttctcatt ccgggctgcg
      961 gttcgcccct tacgcgcgca tcctgcgcgc tcatcgccgc ggccgcggcg cggggcgaac
     1021 cgttcaattc ccccgcgagt actctcgcgc ccctccatac gggttagtaa aagaaaagga
     1081 ccgccggtag gcggtccttt tcttttacta cgtggaggtg aggggaattg aacccctgac
     1141 ctcttgaatg ccattcaagc gctctaccaa ctgagctaca cccccgaagc gagtggcatt
     1201 ctagcgatcg ggccgtagcg tgtcaagcgg atcgcattct ttttttggga cttctgatgt
     1261 ctattgcgtc ggccggcccg ggcgacgcat actggttcta tgagtagcat agtgagacta
     1321 tcggcgtcat gcgccgtggg catagagaag gtcgtggccg gcgagctcga gcggctcggg
     1381 ctggcggctt cgggtagatc ggcgggccgc gtcggcttcg aggctgacaa ggcgggcctg
     1441 gccaaggccc tggtcgggct acggtgcgcc gacagggtgt tcctggaggc gggacggttc
     1501 cgagcccgcg acttcgacga gctgtacgaa ggcttcaagg ccattccctg ggagcgatgg
     1561 gttgggccgc aggacaggct ggtgatcgag aaagcgcggt cgatacgctc ggcgctatcc
     1621 gcccagaacg ccatccagtc agtcggccag aaggccgcct acgagaggct atgcggcgag
     1681 tacggcgtgg gccgtatgcc cgagaccgcc gccacggtgc agacgagggt caggatagag
     1741 cgcgacgagg cgaccgtgga gctcgacctc tgcggcgagg cgctatcgcg acgcggctac
     1801 cgcaagcggc cgaccgaggc gccgctcaag gagagcgtgg ccgctgccgt gcacctgctt
     1861 ggcgccgacc gcatcggtca cggcgtccgt gcaatcgaag acccggcgct gctcaccatc
     1921 ctgcgcgacc gccagattcc gctggagatc aatccaacca gcaacatctg tctgcatgta
     1981 tatcgccgca tcgccgaaca tcctttgccg catctggatc gtatggggct gaagctgacg
     2041 atcaacagcg acgacccgcc gctgttcaac acgaccctgt gcgacgagta tcgcctgctc
     2101 gcaggcgaat ttggctacga ccgcgccggc atcgcccgcc tggcgcgcaa tgcttttgag
     2161 gtaaaccggg ccgagccggc gctcaaacag cggctgttgg ctgagttcga cgcctggtgt
     2221 ctgacggacg atttagacct cttggttcaa acttcttagc actgcttcca actcccctgg
     2281 caaaggggcg acgaactcgc gctcttcgcc gctggagggc aggcgcagcc gcagccggtg
     2341 agcgtgcaga aactggcgct tgaggggcag gtgcggcgca gcaaa
//
LOCUS       DAOOLN010000185         2377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_55479_length_2377_cov_2.669251, whole genome shotgun sequence.
ACCESSION   DAOOLN010000185 DAOOLN010000000
VERSION     DAOOLN010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55479_length_2377_cov_2.669251"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1728)
                     /locus_tag="PLW80_03205"
     CDS             complement(<1..1728)
                     /locus_tag="PLW80_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPB65540.1"
                     /translation="MFKGLTKRAQRVLTVVAQEEARRFHAGELAPEHVLLALLTEGEG
                     TGYRLLSSLKLDMRELALELERAASGRKGGFLLGDAPLSRRLKSMLETAAEEARTMDG
                     EYIGTEHLVIAAARENGSPFDQFLSRYGVFYEQLRAALKLLASPGPYEPRDRSDDAAR
                     QQARPVIQGTLGSAPRRSGGATPLLDEHSRDLTAWARDDRIDPVIGRETETRRVIRIL
                     SRRSKNNPVLIGDPGVGKTAIVEGLALRMAKGDVPDGLMGKRLLVLDMASIVAGTKYR
                     GEFEERLKRIMKEIAASGNVILFIDELHTIIGAGSAEGTIDASNMLKPALSRGELQCI
                     GATTLDEYRKYFEKDAALERRFQTVLVEEPTIEQTQDILSGIKERYERYHGVTYAADA
                     IEAAVRLAGRYVSDRFLPDKAIDVLDEAGARKKVEYPAKGPELSSIEEEIRRLTEEKL
                     ALVSTQNYERAAEVRDRVRELKASLESLRAKTKAASGDGYAVGVDDVRAVVSEMTGIP
                     LERIAEGESERLTSLEEELHRRVVGQDYAVKALAAAVRRSRAGISDPRRPLGSFIFLG
                     PTGVGKTLLA"
     gene            complement(1721..>2377)
                     /locus_tag="PLW80_03210"
     CDS             complement(1721..>2377)
                     /locus_tag="PLW80_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65541.1"
                     /translation="CLGDRPWAFDTDMGYIMSEASRCGSGLSARITVHAPALVLSGLA
                     ETAFKRAMEAGFVVSGSYSSLAASGGSLFDLSLPYASRGPERDSLGRLSSAAKAIAEY
                     ERRARAELLSKSPWDILDTIGRAAGKAGGARLLSRDEATDICSGLRLGLACGVLEGLS
                     LEDATDLWPGLRVKPSPSDASIGPSSQAGSEEPEAARRATSLRLAVAGLRLSQGYIDV
                     "
BASE COUNT          367 a          828 c          770 g          412 t
ORIGIN      
        1 ggccagtagg gtcttgccga cgccggtcgg gccgaggaag atgaaggagc cgagcggacg
       61 cctcgggtcg gagatgccgg ctctggatcg cctgacggcg gcggcgagcg ccttgaccgc
      121 gtagtcctgg ccgaccaccc ggcggtgcag ctcctcctcg aggctcgtca ggcgctccga
      181 ctcgccctcg gcgatgcgct cgagggggat tccagtcatc tccgagacca cggccctcac
      241 gtcgtccacc ccgacggcgt agccgtcgcc cgaggccgcc ttggtcttgg cccgtaggct
      301 ctcgaggctg gccttgagct cgcgcaccct gtcgcgcacc tcggcggccc gctcataatt
      361 ctgggtggat accagggcaa gcttttcctc ggtaaggcgc ctgatctcct cctcgatgga
      421 gctcagctcc ggccccttcg ccggatactc gaccttctta cgggcgccgg cctcgtcgag
      481 tacatcgatg gccttgtccg gcaggaaacg gtcgctgacg tatcggccgg cgagcctgac
      541 ggccgcctcg atcgcgtcgg cggcgtacgt gaccccgtgg tagcgctcgt agcgctcctt
      601 gatgccggac aggatgtcct gggtctgctc tatcgtcggt tcctcgacga ggacggtctg
      661 gaagcggcgc tcgagcgcgg cgtccttctc gaagtacttg cggtactcgt cgagcgtcgt
      721 cgcgccgatg cactgcagct cgcccctcga cagggcgggc ttgagcatgt tcgaagcgtc
      781 gatcgtgccc tcggcgctac ccgccccgat gatggtatgc agctcgtcga tgaagaggat
      841 cacgttgccg gaggcggcaa tctccttcat gatgcgcttg aggcgctcct cgaactcgcc
      901 gcggtacttc gttccggcga cgatggaggc catgtcgagg acgagcaggc gtttgcccat
      961 aagcccgtcc ggcacgtcgc ccttggccat cctgagggcg aggccctcga cgatagccgt
     1021 cttgccgacg ccggggtcgc cgatcagtac gggattattc ttgctacggc gcgacaggat
     1081 gcgtatcacc cgcctggtct cggtctcccg gcctatcacc gggtcgatac ggtcgtcgcg
     1141 agcccaggcc gtcagatcgc gcgagtgctc gtcgagcagc ggggtagcgc cgcccgagcg
     1201 cctcggggcc gagccgagcg ttccctggat gaccggccgg gcttgttgcc gggcggcgtc
     1261 atcggatctg tcacgcggct cgtaaggccc gggcgaagcc agtagcttga gcgcggcacg
     1321 aagttgctcg tagaagacgc cgtaccgcga taggaactgg tcgaacggcg agccgttctc
     1381 gcgcgcggcc gcgatgacga ggtgttccgt tcctatgtac tccccgtcca tcgtcctggc
     1441 ttcctccgcg gcggtctcga gcatcgactt gagccgccta gacaaggggg cgtcgccgag
     1501 cagaaaacct cccttgcggc cggaagcggc ccgctcgagc tcgagcgcca actcgcgcat
     1561 gtccagcttc agggacgata ggagtcgata cccggttcct tcgccttcgg ttaacagggc
     1621 cagtagcacg tgctccggcg ccagctcgcc ggcatggaaa cgcctggcct cttcctgagc
     1681 cacgaccgtg agcacgcgct gcgcccgctt agtcaatccc ttaaacatcg atgtacccct
     1741 gcgataaacg taggcccgcg accgcgagcc tgagactcgt cgccctgcgc gccgcctcgg
     1801 gctcttccga gcccgcctgg ctcgaaggcc caatactcgc gtccgacggc gatggcttga
     1861 cccgtagccc cggccacagg tcggtcgcgt cctcgaggct cagcccctcg aggacgccgc
     1921 aggccagccc gagcctaagc ccggagcata tatccgtagc ctcgtcgcgc gacagtagcc
     1981 tggctccgcc cgccttgcca gcggcccgcc ctatggtatc cagtatgtcc caggggcttt
     2041 tcgacagcaa ctccgccctg gcccgcctct cgtattcggc tatggccttg gccgccgacg
     2101 ataagcgccc caacgagtcg cgctccggcc cgcggctcgc gtacggcaag gacaggtcga
     2161 acagcgagcc tccggaggcg gccaggctcg agtaggagcc ggacacgacg aagcccgcct
     2221 ccatggcgcg cttaaacgcg gtctccgcca gtcctgacag gaccaaggcg ggcgcatgca
     2281 cggtgatgcg tgcagataag ccgctaccgc aacgcgaagc ctcggacatg atgtagccca
     2341 tatccgtatc gaaggcccag ggacggtcgc cgaggca
//
LOCUS       DAOOLN010000186         2368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_55787_length_2368_cov_2.818850, whole genome shotgun sequence.
ACCESSION   DAOOLN010000186 DAOOLN010000000
VERSION     DAOOLN010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55787_length_2368_cov_2.818850"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..1172
                     /gene="recF"
                     /locus_tag="PLW80_03215"
     CDS             102..1172
                     /gene="recF"
                     /locus_tag="PLW80_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254748.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HPB65542.1"
                     /translation="MPFSRIESVSFRNLADGAIQTEAPSVFVVGENGQGKSNFLDAVY
                     TLCYGSSFRGGQDADACRNGERAWSLRGVSPDGSNCVIAWEAGTKAIKEDGKPVRDRK
                     RLVERNPAIVFCHEDMEFSRGEPERRRFFFDQTASLVSVGYIDLLRAYRRTLKARNQA
                     LKDRSLDVLEVLDLQLASYGLGLRDARAAIAREFDGRFAPRFESVSRLGYRVGVSYRP
                     SWRLDEGPDEVVARLAAARDRELALGTSLSGPHRDRFAFTDDSGDFSDRASTGQLRLL
                     SLTLRIAQAEHYAGVSGRKPVLLLDDVLLELDPEKRRRFMEALPEAEQSFFTFLPGEP
                     YRDYLRDGTIVYWTDDGRFSRT"
     gene            1150..1647
                     /locus_tag="PLW80_03220"
     CDS             1150..1647
                     /locus_tag="PLW80_03220"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HPB65543.1"
                     /translation="MDDSRVRKASDLLGALLSPEAAAKADTWSKFFSFWNRAAGENLA
                     AHSRPVDLRNGIVFVEATHPGWIQLLQMRQTQILATIRRSFPDLEVSGIAFRLAKDPS
                     LPGTAKMARPPEPIGAEGQRIDAVPEAASETPSVSETIGKVEDESFRSILSSLAETLG
                     GAGDE"
     gene            1839..2057
                     /locus_tag="PLW80_03225"
     CDS             1839..2057
                     /locus_tag="PLW80_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65544.1"
                     /translation="MDEFRSLLEASELADRYRVSFFESTPENLARNADVQRLLSMASL
                     EPVICIGGKIAYLGGFSPEGLLAELRKR"
     gene            2072..>2368
                     /locus_tag="PLW80_03230"
     CDS             2072..>2368
                     /locus_tag="PLW80_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HPB65545.1"
                     /translation="MELNDEFIAGLEVGKADLLNKIASQLGIRASQAAAVVDLTAEGC
                     TVPFISRYRKERTGELDEVQVRDVVHLHQSLTNLETRRIEVIQAIHGQGKLTEEL"
BASE COUNT          461 a          772 c          740 g          395 t
ORIGIN      
        1 ccgagacctc cagggccgtc accgtggcgc ccgatccgga atccgattat ttccacatcc
       61 tgatgccgat gcaggtagac tagagcggca gacgggactc catgccgttc agccgtatcg
      121 agagcgtatc ctttcgcaac ctagcggacg gggccatcca gacggaggcc ccgtccgttt
      181 tcgtcgtcgg cgaaaacggg cagggcaaga gcaacttcct cgatgccgtg tacacgctct
      241 gctacggctc gagtttccga ggcgggcagg acgccgacgc ctgtaggaac ggggagcgag
      301 cctggtcgct acgaggggtc agccccgacg gttcgaactg cgtcatcgcc tgggaagccg
      361 gaaccaaggc tatcaaggag gacggcaagc cggtacgaga ccgtaagcgc ctcgttgagc
      421 gcaacccggc catcgtgttc tgccacgagg acatggagtt ctcgcgcggc gagcctgaac
      481 ggaggcgctt tttcttcgat cagaccgcga gcctcgtctc ggtaggctac atcgaccttc
      541 tgcgggccta tcgccgaacc ctcaaggcca ggaaccaggc gctcaaggat cggagtctgg
      601 acgtactcga ggtactcgac ctccagctag ccagctatgg ccttggcctg cgtgacgcgc
      661 gcgccgcgat agcccgagaa ttcgatggtc gtttcgcgcc gcgcttcgag agcgtctcaa
      721 ggctcggcta tcgagtcggc gtatcgtatc gtccatcctg gaggctcgat gaaggccccg
      781 acgaggtcgt agccaggctg gctgccgcca gagatcgaga gctggccctc ggcacgagcc
      841 tatcggggcc gcaccgcgac cgtttcgcct ttaccgacga ctccggcgac ttctccgacc
      901 gggcctccac gggccagctc aggctactca gcctgacttt gaggatagcc caggccgagc
      961 actatgccgg cgtcagcgga cgcaagcccg tgctgctcct cgacgacgtg ctcctcgagc
     1021 tcgaccccga gaaacgccgt aggttcatgg aagcgctacc ggaagcagag cagtcgttct
     1081 ttaccttctt acccggggag ccataccggg attacctgcg cgatggcact atcgtatact
     1141 ggacggacga tggacgattc tcgcgtacgt aaagcctccg acctgctcgg cgccctcctg
     1201 tcccccgagg cggccgccaa agccgacacc tggtccaagt tcttctcgtt ctggaaccgc
     1261 gccgcaggag agaacctcgc cgcccactcg cgcccggtcg acctccggaa cggcatcgtc
     1321 ttcgtcgagg ccacccaccc gggatggatc cagctcctcc agatgaggca gacccagatc
     1381 ctcgcgacga tacgccgtag cttcccggac ctcgaggtct ccggcatagc cttcaggctg
     1441 gccaaggacc cgagcctgcc aggcaccgcc aagatggcca ggccacccga gccgataggc
     1501 gccgagggac agaggatcga cgcagtcccc gaggcggcgt cggagacgcc gagtgtcagc
     1561 gagaccatcg gtaaggtcga ggacgagtcg ttcaggagca tactgtcgtc gctcgcggag
     1621 acgctgggcg gagccgggga cgaatagcgc ttgattttta taggagttcc ccgtatcgtg
     1681 acgatcctac gaagcctatg aaccaaacac acgatataga cgtatacggc aaagcgtcag
     1741 agctcatctg cgccggttgt tctcacggaa gcgagggctc ggccgatggc gcgtgctcca
     1801 gttgcgcgcc gggcgcgaag agggcgaccc tcgccctggt ggatgaattc cggagcctcc
     1861 tcgaggcgtc cgagttggcc gatcggtaca gggtgagctt tttcgagtcg acgccggaga
     1921 acctagcgcg caatgccgac gtgcagcgcc tcctgtccat ggccagcctc gagccggtca
     1981 tctgcatcgg cggcaagata gcctacctcg gcggcttcag tcccgaaggt ctgctcgctg
     2041 aactacgcaa gcgataaggt aaggtaccag catggaactg aacgacgaat tcatagccgg
     2101 cctcgaggtc ggcaaggccg acctgctcaa caagatagcc tcccagctcg gcatacgggc
     2161 cagtcaggcg gccgcggtcg tcgatctgac cgccgagggc tgtaccgtgc cattcatcag
     2221 ccgctaccgc aaggagcgca ccggcgagct cgacgaggtg caggtccgcg atgtggtgca
     2281 cctgcaccag agcctgacca acctggagac caggcgcatc gaggtgatcc aggccatcca
     2341 cggccagggc aagctgaccg aagaactc
//
LOCUS       DAOOLN010000187         2366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_55855_length_2366_cov_3.305928, whole genome shotgun sequence.
ACCESSION   DAOOLN010000187 DAOOLN010000000
VERSION     DAOOLN010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55855_length_2366_cov_3.305928"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1509)
                     /locus_tag="PLW80_03235"
     CDS             complement(<1..1509)
                     /locus_tag="PLW80_03235"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPB65546.1"
                     /translation="MAAQSVKTRASSVEPPRVSVPPVVAAAAGLAAALYLPPGSGSRA
                     LASVAAACAASIALSMRFAPSPRSTISLVTKRRLRAVAEPAKGGLRRAASALAAALAA
                     GLCCGAWCHASVEAGSARFSPGLGGPYAPGAAAYEARVASDPTHTAGGLLACELELEA
                     ALGRDGERVTASGRVVVYVRGSSIGGAPRDGRPGEAPVRGQRLRVALRRGLLPPRTGG
                     GSWRDALASPYAFVDAAGVEAIGPPPAAEAWRAALRAGALESLRAAGGEAGPLLEALV
                     VGVRDELDPELSAGFRAAGCAHILALSGQHVGILASFVAVLLGFALGPRRARAVACLL
                     AAAYLYLVGASPSVARAVAMFWATSALLAADRPQPAVVTLSSVFVVAVIVSPAEAHEL
                     SFGLSYLAVAGIGILGGAYEFGLRRWLPPPAAGAVAVGFAALAATAPLSLLAFGTLNL
                     FSPVASALAGLLVAALIWAGLVGSLVVALVPWAAPAVRLACALPYRALVAVVG"
     gene            complement(1565..2155)
                     /locus_tag="PLW80_03240"
     CDS             complement(1565..2155)
                     /locus_tag="PLW80_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65547.1"
                     /translation="MASLKGDYAAFLAEVIRSSPPADKAAALEAGLRGGALPEERRGE
                     LAEVALSVGVSYQSPSSGDQGYILGMRTQAARELTARSWQKASPLAIKHFYDFQVQYN
                     RGQVSKSNFLESIALLGAMGTAEAAQALALYLQLINTETEQGKSFDEQVTLAVVNNLG
                     RLGDKAAFDYLLYIGYLQYPETVKKAARDALQKLRW"
BASE COUNT          378 a          823 c          913 g          252 t
ORIGIN      
        1 cccgacgacg gcgacgaggg ctcggtacgg tagggcgcag gcgagccgga cggccggcgc
       61 ggcccagggc acgagggcga cgacgagcga gccgacgagg ccggcccaga tcagcgccgc
      121 cacgagcagc ccggccaacg cgctcgcgac cggcgagaag aggttgagcg ttccgaaggc
      181 cagtagcgac agcggggccg tggccgcgag cgccgcgaag cccacggcta ccgctcccgc
      241 cgccgggggc ggcagccagc gccggaggcc gaactcgtag gccccgccga ggatgccgat
      301 accggcgacc gccaggtacg acagcccgaa cgacagttcg tgggcctccg ccggagagac
      361 tatcaccgcg acgacgaaca ccgacgacag cgtgacgacc gccggctgcg ggcggtcggc
      421 cgccaggagg gcgcttgtag cccagaacat ggcgaccgcc cgcgctaccg acggcgaggc
      481 gcccacgagg tacaggtagg ccgccgcgag caggcaggct accgcgcggg cgcgcctcgg
      541 gccgagcgcg aagccgagca ggaccgccac gaacgaagcc aggatcccga cgtgctggcc
      601 cgacagcgcg agtatatggg cgcagccggc ggccctgaaa ccggccgaga gctccgggtc
      661 gagctcgtcg cgcaccccga cgacgagggc ctcgaggagg gggccggcct ctccgccggc
      721 cgcccggagc gactcgaggg cgccggcccg gagcgcggcc cgccaggcct cggcagcggg
      781 cggcggcccg atagcctcga cgccggccgc gtcgacgaag gcgtacggcg aggcgagcgc
      841 gtcgcgccag gagccgccgc cggtcctcgg cggcaggagg ccgcgcctta gggcgacgcg
      901 caggcgctgg ccgcgcacgg gcgcctcgcc agggcggccg tcccgaggcg caccgccgat
      961 tgacgagccg cggacgtaga cgacgacgcg acccgaggcg gtgacgcgtt ctccgtcgcg
     1021 gccgagcgcc gcctccagct ccagctcgca ggcgaggagg cctccggccg tatgggtcgg
     1081 gtcggaggcg acccgagctt cgtaggcggc cgcgccgggg gcgtacggcc cgccgagccc
     1141 cggactgaaa cgcgccgagc cggcctcgac gctggcatgg caccaggccc cgcagcacag
     1201 cccggcggcc agagccgccg cgagcgccga cgccgctcgc cggaggccgc ctttcgccgg
     1261 ctcggcgacg gcgcgcaggc gccgtttcgt aaccagggat atcgtcgacc tggggcttgg
     1321 agcgaaacgc atcgagagcg cgatggaagc cgcgcacgcg gcggcgacgc tggccagggc
     1381 cctcgaaccg gaaccgggag gcaggtagag cgcggcggcc agcccggccg ccgccgcgac
     1441 gaccggcggc acggagacgc gcggaggctc gacggaggac gcgcgcgttt tgacggattg
     1501 agcggccacg accggatacg cggctcggag cctcggcgcg tgcgctatcc aggaaggccg
     1561 agcgttacca gcggagcttc tggagcgcgt ccctggcggc cttcttgacc gtctcgggat
     1621 actggaggta gccgatgtac aggagatagt cgaaagccgc cttgtcgccg aggcggccga
     1681 gattgttgac gaccgcgagc gtgacctgct cgtcgaagga cttaccctgc tccgtctcgg
     1741 tgttgatcag ctggaggtag agcgccaggg cctgggcggc ttcagcggtg cccatcgctc
     1801 cgagcagcgc gatcgactcg aggaagttcg acttggagac ctggccgcgg ttgtattgca
     1861 cctggaaatc gtagaagtgt ttgatagcga gcggcgaggc cttctgccac gatcgggcgg
     1921 tcagctccct ggcggcctgg gtacgcatac ccaggatata gccctggtcg cccgacgacg
     1981 ggctctggta cgagacgccg accgacaacg ccacctcggc cagctcgccc cggcgctcct
     2041 cgggcagggc gccgccacgc aggccggcct cgagggccgc ggccttgtcg gcgggcgggc
     2101 tcgatcggat gacctcggcg aggaaggccg cgtagtcgcc cttgagcgac gccatcgcgg
     2161 cgccggcccg gtcggtgacg gccttgccga tggacgcggt ataggccgcg aagaggtacg
     2221 ggaacgatta cgagtcgccg aggcggccga gcgcgaagac ggcggcgtcg agcacggcca
     2281 ggtcgatccg gacgccggac ttggcgagcg acacctgagt gtcgacgaag gcgttgagct
     2341 ccttgaggat ggcggggttg cctacg
//
LOCUS       DAOOLN010000188         2365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_55896_length_2365_cov_2.987013, whole genome shotgun sequence.
ACCESSION   DAOOLN010000188 DAOOLN010000000
VERSION     DAOOLN010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55896_length_2365_cov_2.987013"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PLW80_03245"
     CDS             <1..306
                     /locus_tag="PLW80_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65548.1"
                     /translation="GVGLSVAPGLLAEGSTARRTSVAAALVRELVAWCAAPSLELGRW
                     DSLAPPAGSHMSVELWNGERRAFEPVGFDTRGDLTGADGTRLSIGEYRSHSIEGEYQ"
     gene            303..929
                     /locus_tag="PLW80_03250"
     CDS             303..929
                     /locus_tag="PLW80_03250"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HPB65549.1"
                     /translation="MKQNGFKLVLSSLFAAMIAMGAVFSLPLPPPLPPITLAVFFSLL
                     SGLLLGPRWALATTGLYLGLGAVGLPVFANGAGGLGQLAGPTGGFLVGYVAAAVVAGA
                     VSDRRRWGFGRAVAGALAGVAALYIVGLPRFRAVLDAGPDRSVTMGAAFLMMAPYLVG
                     DMAKALAAAGLVRAMRPLLAEYLPARDRARGRGEVVPGPGGPNDEARA"
     gene            913..1668
                     /locus_tag="PLW80_03255"
     CDS             913..1668
                     /locus_tag="PLW80_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB65550.1"
                     /translation="MKLELRNLVKTFADGTRGLDGASLSIETPSFVALSGQNGSGKSL
                     LIRHALGLEEADSGEVLVDGRPLHSRLAEARRRIALVFQEPEHQILGVTVREDVEFGP
                     RAAGERPESYAPRVEAALEAAGLAGYGPRLCAGLSGGEKRRLAVASALVSEPELIVLD
                     EPFNGLDWAGASDLLAVVLRLRASGVGVLVVTHDLEKCLAHADRLVVMDAGRIVRDDA
                     PAALWEELPSLGLRSPAGGYERLAEMSWLTEGA"
     gene            1833..>2365
                     /locus_tag="PLW80_03260"
     CDS             1833..>2365
                     /locus_tag="PLW80_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65551.1"
                     /translation="MWRFVLYLALQLAILASPPTWLALLAVVVALLGLVEGKDWPRWI
                     LRSRFALVAALLPAAVGFPIGTVGGLGEAGFGAVLAAWVPALSRSVRLALVLASAAWL
                     SGGMSPVELRDALITILRPLGNRLGGRVARSASLTMAFLPWTAEEARAADEAARLRGS
                     DPSRRPVIRRRRSRRSS"
BASE COUNT          278 a          798 c          887 g          402 t
ORIGIN      
        1 ggcgtcggcc tgagcgtcgc gcccggcctc cttgccgaag ggtccaccgc gcggcggacg
       61 tcggtggcgg ccgcgctcgt gcgcgagctg gtcgcatggt gcgccgcgcc gagccttgaa
      121 ctcggccgtt gggatagcct ggctccgccg gctggtagcc atatgtccgt cgagctatgg
      181 aatggcgagc gccgcgcctt cgaaccggtc ggcttcgata cgcgcggcga cctgaccggc
      241 gccgacggga cgcgcctgtc gatcggcgag taccgctcgc atagcattga aggggaatat
      301 caatgaaaca gaacggattc aagctggtgc tgtcgtcgct gttcgccgcg atgatagcga
      361 tgggcgcggt cttctcgctg cccttgccgc cgccgttgcc accgatcacg ctggcggtgt
      421 tcttctcgct actgtccggg ctgctactgg ggccgcgctg ggcgttagcc actaccggcc
      481 tctacctggg gctcggcgcg gtcgggttgc cggtgttcgc caatggagcc ggcggcctcg
      541 gtcagctggc aggtccgacc ggcggcttcc tcgtaggcta cgtcgcggcc gccgtggtag
      601 ccggagccgt gtcggaccgg cgtcgctggg gtttcggacg ggccgtggcc ggtgccctgg
      661 caggtgtggc ggcgttgtat atcgtcggcc tcccccgatt cagggccgtg ctcgacgccg
      721 gtcccgaccg tagcgtgacg atgggagccg ccttcctgat gatggcgccg tacctggtag
      781 gcgatatggc taaggcgctc gccgccgccg gactcgtgcg ggctatgcgg ccgctcctgg
      841 cggagtactt gccggcgcgg gaccgggcgc gtggccgagg cgaggtggta cccggcccgg
      901 gagggccgaa cgatgaagct cgagcttagg aacctggtca agaccttcgc ggacgggacg
      961 cggggtctcg acggggcgag cctatcgatc gagacgccgt ccttcgtcgc gctttcgggg
     1021 cagaacggct cgggcaagtc gctgctgatc cgccatgcgc tcggcctcga ggaagctgac
     1081 tcgggcgagg tcctcgtcga cggccggccc ctacatagcc gccttgccga ggccaggcgc
     1141 cgcattgccc tcgtgttcca ggagcccgag caccagatcc tcggcgtgac cgtccgcgag
     1201 gatgtcgagt tcggcccgag agcggccgga gagcggcccg agtcgtacgc cccgcgcgtc
     1261 gaggcggcgc tcgaggccgc gggcctggcc ggctatggcc cgcgcctgtg cgccggcctg
     1321 tcgggcgggg agaagcgacg gctggcggtc gcctcggccc tggtatccga acccgagctg
     1381 atagttctcg acgagccgtt caacgggctc gattgggccg gcgcctccga ccttctcgcg
     1441 gtggtattgc gcctgcgcgc ctcaggtgtc ggggtgctgg tcgtgactca cgacctggag
     1501 aagtgcctgg cgcacgctga ccgcctggtc gtcatggacg ccggccgtat cgttcgcgac
     1561 gacgcccctg ccgcgctctg ggaagagctc ccctcgctcg gcctcagaag cccggccggc
     1621 gggtacgagc ggctggcgga gatgagctgg ctgacggagg gcgcgtgaag gccgagccat
     1681 ggcgcgtcgg cgtcgtccgc ctggcgggcc gacgtcccgg cggaagggtc ggcgggatga
     1741 tcgcgcggac tacgcgcgtc acgcggacta cgcgcgtcac gcggactacg cgcgccacgc
     1801 ggactacgcg cgccacgcta gcggtacgcg gtgtgtggcg cttcgttcta tacttggccc
     1861 tgcagctggc catactcgct tcgccgccca cctggctggc cctgctggcc gtcgtcgtgg
     1921 cgcttctcgg cctggtcgag ggcaaggact ggccgcgctg gatcctacgg tcgcgcttcg
     1981 cgctcgtggc ggccctcctt cccgccgccg tcgggttccc catcggtacg gtcggcggcc
     2041 tcggcgaagc tgggttcggc gcggtactgg ccgcctgggt tccggcgctg tcgcggagcg
     2101 tgcgtctggc ccttgtattg gcgtccgcgg cctggctgtc cggtggcatg agtcccgtgg
     2161 agctacgcga cgcgctgata acgatactgc ggccgctcgg gaatcggctc ggcggccgcg
     2221 tcgccaggag cgcgtcgctg acgatggcct tcctgccctg gacggccgag gaagcccgcg
     2281 ccgccgacga ggcggctagg ctgcgcggct ccgacccgtc gcgcaggccg gtgatccgtc
     2341 gcaggagatc gaggcggtcg tcgta
//
LOCUS       DAOOLN010000189         2352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_56295_length_2352_cov_2.521114, whole genome shotgun sequence.
ACCESSION   DAOOLN010000189 DAOOLN010000000
VERSION     DAOOLN010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56295_length_2352_cov_2.521114"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..1401
                     /locus_tag="PLW80_03265"
     CDS             235..1401
                     /locus_tag="PLW80_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65552.1"
                     /translation="MGSQERKAVIELPVKVVLTDSGTTYFIKNNKKLRKFKLADNVEE
                     YGILLDHFTPASLQRMMLIDYVSKVEISDSEFVKIRQEVMDISKLVTYSMMYRQYDSY
                     IFQRVLSSDVIKNWNRKNPANIIDEKTKINDVFLQAFIKDKDKEIAEIKRTVLAPMYA
                     LINRNSNLLPEEKNIQLLLSEKFLNTLRPFTWFIIAKFHDSDGYDTLIKDIRTSLAEY
                     MEKAKIAEYVALNVMELAANAENNNLKREAKAIFKGAVDMNAVLFDPNVRHQVLESLQ
                     HRGELVSISWKLGSKGTSIGTQGRLHITIYNKESEYEKMKEAFDEKKNADLKKRTLQD
                     FYKALPEGESNTDLGLYYLSYLSEACEKVNVKFESFVNQISGSDLTVITMAINL"
     gene            1515..>2352
                     /locus_tag="PLW80_03270"
     CDS             1515..>2352
                     /locus_tag="PLW80_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPB65553.1"
                     /translation="MAKTKYVYYFGDGKAEGDAKMKEALGGKGANLAEMTNLGIPVPP
                     GFTVSTDVCAAFYENKRKYPDGLEAEVAEHLARLEKSMGKKLGDAVDPLLVSVRSGAA
                     QSMPGMMDTILNLGLNDKAVEGLAKKTGNPRFAYDSYRRFIQMYGDVVLEVAHDEFEH
                     ALAAAKKAKKVELDTGLSADDLKKLVETYKAIVKKHTKKDFPQNPQDQMWGAMGAVFG
                     SWMNERAIKYRDLNGIKNLKGTAVNIQSMVFGNWGDDSGTGVCFTRDPSTGKNEFYGE
                     FLM"
BASE COUNT          579 a          717 c          653 g          403 t
ORIGIN      
        1 gaacgcgccc gagatctcga ccgtcctcgg gtcgttcgag gcttcctgcg caccgagcgt
       61 aaggctggcg agcgagccgg aggcgataat ggctaatacg gcgatcgata aatgccgaaa
      121 catgtcgtat ggcgatctta ggggcgagcc cgacgccagg tcaataccgt tttacacgtg
      181 ggcgacgatg ggatatactg caacgacgga tcggcaacgg aggtatgaat caggatgggg
      241 tcgcaggaac gcaaagccgt catcgaactg cccgtcaagg tcgtcctgac cgatagcggc
      301 accacctatt tcatcaagaa caacaagaaa ctccgaaaat tcaaactcgc ggacaacgtc
      361 gaggaatacg gcatcctgct cgaccatttt acgcccgcca gcctgcagcg catgatgctg
      421 atcgactacg tatcgaaggt cgagatatcg gacagcgagt tcgtcaagat acgccaggag
      481 gtgatggata tctccaagct ggtgacctat tcgatgatgt accgccagta cgacagctat
      541 atcttccagc gggtactctc gagtgacgtc ataaaaaact ggaaccgcaa gaaccccgcc
      601 aacatcatcg acgagaaaac caaaattaac gacgtcttcc tccaggcctt catcaaggat
      661 aaggacaagg agattgcgga gatcaagcgc acggtgctcg ccccgatgta cgcgctgatc
      721 aaccgcaact cgaacctcct gcccgaggag aagaacatac agctcctcct gtcggagaag
      781 ttcctcaaca cgctcaggcc gtttacctgg ttcatcatag ccaagttcca cgattccgac
      841 ggctacgaca cgttgatcaa ggatatccgg acgagcctcg ccgaatacat ggaaaaagcc
      901 aagatagccg agtatgtggc gctcaacgtg atggagctgg cggccaacgc cgagaacaat
      961 aacctgaagc gagaagccaa ggccatcttc aagggcgcag tcgacatgaa cgccgtcctg
     1021 ttcgacccga acgtgcgcca tcaggtgctc gagtcgctcc agcaccgggg cgagctggtg
     1081 tccatatcat ggaagctcgg ctccaagggc acctcgatcg ggacccaggg ccggctgcat
     1141 ataacgatct ataacaagga atccgagtac gagaagatga aggaggcgtt cgacgagaag
     1201 aaaaacgccg acctcaagaa gcgcaccctg caggacttct acaaggccct gccggagggc
     1261 gagtcgaata ccgacctcgg gctctactac ctgtcctacc tgtccgaggc ctgcgagaag
     1321 gtcaacgtca agttcgagtc cttcgtcaac cagatctccg gctccgacct gaccgtcatc
     1381 acgatggcca tcaacctgta gcccgagggg cacttcgccc gaaaccctcc gtccctcgat
     1441 accctccctt acggtttctt gacagaggct gtggtttcct atagcctcat gggttgaacg
     1501 gaggatttta ctcgatggca aagaccaagt acgtatacta cttcggcgac ggcaaggccg
     1561 aaggcgacgc caagatgaag gaagcgctcg gcggcaaggg cgccaacctc gccgagatga
     1621 ccaacctggg catcccggtc cccccgggat tcacggtgtc caccgatgtc tgcgcggcat
     1681 tctacgagaa caagcgcaag taccccgatg gcctcgaggc cgaggtagcg gagcaccttg
     1741 ccaggctcga gaagtccatg ggcaagaagc tcggcgacgc cgtcgacccc ctgctcgtat
     1801 ccgtacgctc cggcgcggcc cagtccatgc ccggcatgat ggacaccatc ctcaacctgg
     1861 gcctcaacga caaggccgtc gagggcctgg ccaagaagac cggcaatccc cgcttcgcct
     1921 acgattcgta ccgccgtttc atccagatgt acggcgacgt cgtcctcgag gtcgcgcacg
     1981 acgaattcga gcacgcgctc gcggccgcca agaaggccaa gaaggtcgag ctcgacaccg
     2041 gcctctccgc cgatgacctc aagaagctcg tcgagaccta taaggccatc gtcaagaagc
     2101 acaccaagaa ggatttcccc cagaatccgc aggatcagat gtggggcgcc atgggagccg
     2161 tcttcggctc ctggatgaat gagcgggcca tcaagtaccg cgacctcaat ggcataaaga
     2221 acctcaaggg caccgccgtc aatatccagt cgatggtgtt cggtaactgg ggcgacgact
     2281 ccggcaccgg cgtctgcttc acccgcgacc cgtccaccgg caagaacgag ttctacggcg
     2341 agttcctgat ga
//
LOCUS       DAOOLN010000190         2350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_56358_length_2350_cov_2.991285, whole genome shotgun sequence.
ACCESSION   DAOOLN010000190 DAOOLN010000000
VERSION     DAOOLN010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56358_length_2350_cov_2.991285"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="PLW80_03275"
     CDS             <1..877
                     /locus_tag="PLW80_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65554.1"
                     /translation="RFALAAAAAAALAAVPLVAGAQSVAASGSAAASISPASSLPYAF
                     NEAVVSSRTGLSGRLTVYGLGSDGERYELYRAELGYADAATVTFYAWPSLEAIVTEGL
                     DGDGLPFEARARLERSGFGPSPGAETEPDLSTLEFPMRGRMPGALEADRRPWPLALLA
                     LWAALFSLAPLAVLGGRGRLGTAGRSGRYATRRGPAVAETVAAVSATIAIAVLAFALG
                     HARPDLRSAPDDSGATLLGVRSPLEPLPLSSFDGYARLRFQRPPLVIAGDNGRPALAP
                     APFQAAWALPGRPRE"
     gene            870..1343
                     /locus_tag="PLW80_03280"
     CDS             870..1343
                     /locus_tag="PLW80_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65555.1"
                     /translation="MSRRLGVASAAMVVGPLLVAALALPGEADVAAAASIAGGLPLAW
                     AAAVSLAASLDATGLAIALAGALPFGAWALASYAPRPSWPDLVALASAAVAGSVSAFA
                     LERSARAGHGRARLVVPIVAMASCAAASELAGPWSGAAVSSLVVVLSSLAFARPR"
     gene            1340..>2350
                     /locus_tag="PLW80_03285"
     CDS             1340..>2350
                     /locus_tag="PLW80_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65556.1"
                     /translation="MRMMSARRAVRRLVLRDALGRLRFRSVAWLGTAFAAWSLTLLAS
                     VSLLDTRLMPSPLAFNAIVMAIVGSLPAFLAYPARRSWGMDAIRAADSETSIEAWLGY
                     RGGPAERLLERRALEALGLATIGVPQATRRPASARRFVAALYAVGLASFVAAQIASIG
                     SGCGLSLGYPEREIVPAVSDASVAIEDGYPAVVEAEAEEVDEKPAEDGSAEPRPSRSA
                     GEADENRLEEPDFVAASDGRPGDGAAEAAATDQAKAPSRSAVAGAGTRRGGAVSSGDG
                     SGDGSGGEASSPGYEGRGRAIEPSPMLDYRARFERQYAEATGVETAIGPEPSAETVSA
                     AIT"
BASE COUNT          270 a          856 c          870 g          354 t
ORIGIN      
        1 ccgcttcgct ctggcggccg ctgcggcggc cgcgctggcc gccgtccccc tcgtcgccgg
       61 ggcgcagtcg gtcgcggcgt cgggttcagc cgccgcctcg atatcgcccg cttcgtcgct
      121 accctacgcc ttcaacgaag ccgtggtctc gagccgtacg ggcctatcgg gccgcttgac
      181 ggtctacggc ctcggttcag atggagaacg gtatgagctc tacagggccg agctcggcta
      241 cgccgacgcc gcgaccgtca ccttctatgc ctggcccagc ctcgaggcca tagtcaccga
      301 aggtctggac ggcgatgggc tgcccttcga ggctcgcgcg cggctcgaac gctccggctt
      361 cgggcccagt cccggggcgg agaccgagcc ggacctgtcg acgctcgagt tcccgatgcg
      421 cgggcgcatg ccgggtgcgc tcgaggccga caggcggcca tggccgctcg ccctgctagc
      481 gctctgggcc gccctgttct cgctggcgcc gctcgccgtt ctcggcgggc gaggccgact
      541 tggtaccgcc gggcgctcgg gacggtacgc gacgaggcgc ggccccgcgg tcgccgagac
      601 ggtcgcggcc gtctcggcga cgatcgcgat cgccgtgctg gccttcgcgc tcggccatgc
      661 ccggccggac ctacgctcgg ccccggacga tagcggcgcg accctgctcg gcgtccgctc
      721 gccgctcgaa cccctgccgc tgtcgtcgtt cgacggctac gcgaggctga ggttccagcg
      781 gccgccgctc gtgatagcgg gcgacaacgg ccggccggcg ctcgccccgg ccccgttcca
      841 ggcggcctgg gcccttccag ggaggcctcg tgagtaggcg tttgggcgtc gcgagcgcgg
      901 cgatggtggt gggtccgctc ctggttgcgg ccttggcgct accgggtgag gccgacgtgg
      961 ccgcggccgc ctcgatagcc gggggcttgc ccctcgcctg ggccgccgcc gtgtcgctcg
     1021 cggccagcct ggacgcgacc gggctggcca tcgccctggc cggcgccctg ccgttcggcg
     1081 cctgggcctt ggcctcctac gcgcctaggc cgtcctggcc cgacctggtc gcgctggcct
     1141 cggcggccgt ggccggctcg gtttcggcgt tcgcgctgga acgctcggcc cgggccggac
     1201 acggccgcgc gcgcctcgtc gtacccatcg tcgccatggc cagctgcgcg gccgcgtccg
     1261 agctggccgg tccctggtca ggcgcggccg tatcgtcgct cgttgtcgtc ctatcgtcgc
     1321 tagccttcgc gaggccccga tgaggatgat gagcgccagg cgtgcggtcc ggcgcctcgt
     1381 cctaagggac gcgctcgggc ggctacggtt ccgctcggtc gcctggctcg gaaccgcttt
     1441 cgccgcctgg tcgctgacgc tgctggcgtc ggtcagcctg ctcgacacga ggctcatgcc
     1501 gtccccattg gcgttcaacg ccatcgtgat ggcgatcgtc ggctcgctcc cggccttcct
     1561 ggcctatccg gcgaggcgat cctggggcat ggacgccata cgggcggccg atagcgagac
     1621 ctcgatcgaa gcctggctcg gctatcgggg tggccccgcc gagcggctgc tcgaacggag
     1681 ggccctcgaa gcgctgggcc tcgcgacgat aggagtgccg caggcgacga ggcgtccggc
     1741 gagcgcgcgc cgtttcgtag cggcgctgta cgccgtcgga ctcgccagct tcgtggccgc
     1801 ccagatcgcg tcgataggct cgggctgcgg cttatccctc ggttatccgg aacgcgagat
     1861 agtcccggcc gtatcggacg cgtcggtcgc tatcgaagac gggtatccag ccgtcgtcga
     1921 ggccgaggcc gaggaagtcg acgaaaagcc tgccgaggac ggatcggccg aacctcgccc
     1981 atcccgaagc gccggggaag ccgacgagaa caggctcgag gagcccgact tcgtggcggc
     2041 gtccgacggc cgaccgggcg acggagcggc cgaggcggcg gcgacggatc aggccaaggc
     2101 accgagccgt tcggcggtgg ccggggccgg gaccaggcga ggcggcgccg tatcctcggg
     2161 cgacggctcg ggcgacggtt ccggcggcga ggcctcgagc cccggctacg aggggcgggg
     2221 gcgggccatc gagccgagcc cgatgctgga ctatcgcgcg cgcttcgagc gccagtatgc
     2281 cgaggcaacc ggcgtcgaga cggccatagg cccggaaccg agcgccgaga cggtctcggc
     2341 cgccataacc
//
LOCUS       DAOOLN010000191         2350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_56365_length_2350_cov_2.660131, whole genome shotgun sequence.
ACCESSION   DAOOLN010000191 DAOOLN010000000
VERSION     DAOOLN010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56365_length_2350_cov_2.660131"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1450)
                     /locus_tag="PLW80_03290"
     CDS             complement(<1..1450)
                     /locus_tag="PLW80_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HPB65557.1"
                     /translation="MPEFFETDDIVKSYDGAIVRRIFAYVKPYKGLLAAATLTLALST
                     IGELVTPVLVQRSIDEALVVSWVSLDTELASGPRLRGVDVPSDAPRSGGRLYLRSGEL
                     ASLSSRVREDLERDGSLDPGPYYLAQVVDAEALPDVVSADLGVPGGPVLADGWLVAPS
                     AYVASLSAEDARALRSEDRRVVRLYGWTLLAVLIVVLFSTFAQAWTTSLIGQNVMKDL
                     RMDLFKRTSRRSIASLSRQPVGRLVTRLTSDVETINEFFTNVVVSFIKDLSLMAGSLV
                     VLVAMSPRLAFIAIATLPPVLVATGVSRRKARDAFRRQRTWLSKVNAFIAEHIAGVAI
                     VKLFGRERASSEAFARDDAELMRANLGEMYVFAVFRPVIEFLSSVSIAVILWFGAGFH
                     GSALITLGTLIAFVNLIRMFYSPLQDISEKYTMLQSAMAGGERVFKLLDDDERIPDEP
                     TASLTVPVRGRIEFDHVWFAYKGEDWVLRDLSF"
     gene            complement(1443..>2350)
                     /locus_tag="PLW80_03295"
     CDS             complement(1443..>2350)
                     /locus_tag="PLW80_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB65558.1"
                     /translation="PMIGAGFTVNMLQQGAASLKRVNEVLHSEPEPRFAPGALQVAVA
                     APRGGVAFDGLTVLYPGSSRPALDGVELAIGEGRVLGVLGRVGSGKSTLVKTLPRIVE
                     PPRGAVRFGGVDVGSYPLEALRSAIGFVPQDSFLFSDSLRANICFADPDLSASRFEEV
                     VAIAALERDEKLFARGWDTVVGERGLTLSGGQKQRVAIARALARDPELLVLDDSLSAV
                     DAETEEAIVEALLADRAGKTTIIISNRVSTLRRADLVAVLDGGRLAQFGSPAELAGAD
                     GFYAEIAALQALSAEPEARGERADA"
BASE COUNT          400 a          798 c          803 g          349 t
ORIGIN      
        1 tgaacgacag gtcgcgcagt acccagtcct cgcccttgta cgcgaaccag acatggtcga
       61 actcgatgcg cccccgaacc gggacggtca ggctcgccgt cggctcgtcg gggatgcgct
      121 cgtcgtcgtc cagcagcttg aagacccgct ctccgccggc catcgcgctc tggagcatcg
      181 tgtacttctc ggagatgtcc tggagcggcg agtagaacat cctgatcagg ttgacgaagg
      241 cgatgagcgt gccgagcgtg atcagggcgg aaccgtggaa gccggcgccg aaccacagga
      301 tcacggctat cgacaccgac gataggaact cgatgaccgg cctgaacacc gcgaacacgt
      361 acatctcgcc gaggttcgcc cgcatcagct cagcgtcgtc gcgcgcgaag gcctcggaag
      421 aggcccgctc gcgaccgaac agcttgacga tcgcgacgcc ggctatgtgc tcggctatga
      481 aggcgttcac cttggacagc caggtccgct gcctgcggaa cgcgtcccgg gccttgcgcc
      541 tggagacgcc ggtcgcgacg aggaccggcg gcagggtggc gatcgctatg aaagccaggc
      601 gcgggctcat cgcgaccagc acgacgaggg atccggccat caggctcagg tccttgatga
      661 acgatacgac gacgttcgtg aagaattcgt tgatcgtctc gacgtcgctc gtcagcctcg
      721 tgacgagccg gcctaccggc tgcctggaca ggctggctat ggagcggcgg ctcgtccgct
      781 tgaacaggtc catgcgcagg tccttcatca cgttctggcc tatgaggctc gtggtccaag
      841 cctgggcgaa ggtcgagaag aggacgacga tcaggacggc gaggagggtc cagccgtaca
      901 ggcggacgac ccgccggtcc tcggagcgca acgctcgggc gtcctccgcc gatagcgacg
      961 cgacataggc cgacggcgcg acgagccagc cgtcggcgag gaccgggccg cccgggactc
     1021 caaggtcggc ggataccacg tccggcaagg cctcggcgtc tacgacctgc gccaggtagt
     1081 acgggcccgg gtcgagcgag ccgtcgcgtt cgaggtcctc tcgcactcgg gacgatagcg
     1141 aggccagctc gccggagcgc aggtacagcc tgccgccgga tcgcggcgcg tcggacggta
     1201 cgtctacccc tctcagcctg gggcccgagg ctagctcggt gtccagagag acccacgaaa
     1261 cgacgagggc ctcgtcgatg gagcgctgga cgagtaccgg cgtgacgagc tcgccgatgg
     1321 tggacagggc cagtgtcagc gtcgccgccg ccaggaggcc cttgtatggc ttgacgtagg
     1381 cgaagatgcg cctgactatg gctccgtcgt agctcttgac gatgtcgtcg gtctcgaaga
     1441 attcaggcat cggctcgctc ccctcgggcc tcgggctcgg cgctcagggc ctggagcgcc
     1501 gctatctcgg cgtagaagcc gtccgccccg gccagctcgg ctggcgaacc gaactgggcg
     1561 agccggccgc cgtcgagcac ggccaccagg tccgcccgcc tgagcgtcga gacccggttg
     1621 gatatgatga tggtcgtctt gccggccctg tcggccagca acgcctcgac gatggcttcc
     1681 tcggtctcgg cgtccaccgc cgacagcgaa tcgtcgagca cgagcagttc cggatcgcgc
     1741 gccaaagccc tcgctatggc cacccgctgc ttctggccgc ccgagagggt caggccccgc
     1801 tcgcccacga ccgtgtccca gccgcgggcg aatagcttct cgtcgcgctc gagcgccgcg
     1861 atggccacga cctcctcgaa acgcgaggcc gacaggtcgg ggtcggcgaa gcatatattg
     1921 gcgcgcaggc tgtcggagaa caggaagcta tcctggggca cgaagcctat ggccgagcgc
     1981 aacgcctcga gcgggtacga gccgacatcg acgccgccga agcggaccgc cccgcgcgga
     2041 ggctcgacga tgcgcggcaa cgtcttgacc agggtcgact tgccgctgcc cacccggccg
     2101 agcacgccga ggacccggcc ctcgcctatg gccagctcga cgccgtcgag ggcggggcgc
     2161 gagctaccgg gatacaggac ggtcagccca tcgaacgcca cgccgcccct gggcgcggcg
     2221 accgcgacct ggagggcgcc tggcgcgaat ctgggctccg gctcgctatg cagcacctcg
     2281 ttgacccgtt tgaggctggc ggctccctgc tggagcatgt tcacggtaaa gccggcgcct
     2341 atcatcggcc
//
LOCUS       DAOOLN010000192         2348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_56445_length_2348_cov_3.187963, whole genome shotgun sequence.
ACCESSION   DAOOLN010000192 DAOOLN010000000
VERSION     DAOOLN010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56445_length_2348_cov_3.187963"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /locus_tag="PLW80_03300"
     CDS             <1..1364
                     /locus_tag="PLW80_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65559.1"
                     /translation="SPAYSFPADKRLLMHWNMRDEIKALYADPAGLPGQRLLYKAMER
                     VVEGAIPRAFVDSRELTWDPFSGEAHTADGSRANPEREGDGRYDRLLAIFRAERGVDQ
                     WCPETPTLIARKFGVEREIPERQVERLFVDALSAPLFGRAADELRRRLGRPLEAFDAW
                     YKGFGDDSSPDEAALDAITRARYPDAKAFKADIPRILGALGFDADLARWLSERIEVDQ
                     SRGSGHAMPAAKRSDSVRLRTRVPATGMDYKGYNIACHELGHNVEQVLSLHGVDEYFL
                     AGVPNNAFTEAFAFVFQARDRMLLGLEGAPSSPSALGDYWSTCEIAGVGLVDMEVWRW
                     MYAHPDCTARDLREATMAIARSVWNRYFAPAYGLKDALILGTYSHMVELSMYLPDYPL
                     GHLVAFQIEAAMAGKPIGPEMSRMCRLGRLAPNQWLREATGSELSAEPLIAAAERELA
                     AAR"
     regulatory      1579..1709
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            1701..>2348
                     /locus_tag="PLW80_03305"
     CDS             1701..>2348
                     /locus_tag="PLW80_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPB65560.1"
                     /translation="MYDSLMYGSEPSPGLPPNEPVRSYAPGSLERSAIDAELKAMAAN
                     PIEIPLVIGGKEVRTGRIKEIRAPYDRSLVLGRYHEAGEAEAIAAAEAAAVAYREWSE
                     TPFQERAAVFRKAADLISAGWWKALNASTMLGQSKNAYQAEIDSTCETADFFRINPAL
                     AERLYAEQPRSGPGERNLSDYRPLDGFVYAVTPFNFTAIAANLPSAPALMGNTVVW"
BASE COUNT          369 a          822 c          771 g          386 t
ORIGIN      
        1 gctccccggc atatagcttc ccggcggata agcgcctcct gatgcactgg aacatgcgcg
       61 atgagatcaa ggcgctgtac gccgatccgg cgggcctgcc cggccagcgg ctcctgtaca
      121 aggcgatgga gcgcgtcgtc gaaggcgcga tcccgcgggc cttcgtcgac tcgcgcgagc
      181 tgacctggga cccgttctcc ggcgaggcac ataccgccga cggttccagg gccaatcccg
      241 agcgcgaggg cgatgggcgc tacgatcgcc tgctcgccat cttccgggcc gagcgcggcg
      301 tcgaccagtg gtgccccgag acgccgaccc tcatcgcccg caagtttggt gtcgagcgcg
      361 agatacccga gcgccaggtc gagcgcttat tcgtcgacgc gctgtcggcg ccgctgttcg
      421 gcagggcggc ggacgagctc cgtcgtcgcc tcggccgccc cctcgaggcc ttcgacgcct
      481 ggtacaaggg cttcggcgac gactcatcgc ccgacgaggc cgccctcgac gctatcacgc
      541 gggcgcgtta tcccgacgcg aaggccttca aggcggatat cccgcgcatc ctcggcgcgc
      601 tcggtttcga cgccgacctg gcccgctggc tgtccgagcg catcgaggtc gaccagtcgc
      661 gcggctcggg acacgccatg ccggcggcca agcgctccga ctccgtccgc ctgcgcaccc
      721 gggtgccggc caccggcatg gactacaagg gctacaacat cgcgtgccac gagctcggcc
      781 acaacgtgga gcaggtgctg tccctgcacg gcgtcgacga gtatttcctg gccggggtgc
      841 ccaataacgc gttcaccgag gccttcgcgt tcgtgttcca ggcccgcgac cgcatgttgc
      901 tcggcctcga gggggctcca tcgtcgccct cggcccttgg cgactactgg agtacctgcg
      961 agatagcggg cgtcggtctc gtcgacatgg aggtctggcg ctggatgtac gcccatcccg
     1021 actgcacggc ccgcgacctc cgcgaggcga cgatggcgat tgcgcgttcg gtctggaacc
     1081 gctacttcgc cccggcctac ggccttaagg acgcgctgat cctcggcacg tactcgcaca
     1141 tggtcgagct gtcgatgtac cttcccgatt acccgctcgg tcatctcgtc gccttccaga
     1201 tcgaggccgc aatggccggc aagccgatag gcccggagat gagccgcatg tgcaggctcg
     1261 gccgtctggc gccgaatcag tggctgcgag aggctaccgg ctccgagctg tcggcggagc
     1321 cgttgatcgc ggcggccgaa cgggaactgg ccgccgccag gtagcgctcg ccgggagttg
     1381 cggcgcttgc cggttcgtac ggctccgcta ggcgctctga tcaataagca gtatattgat
     1441 atatcagtcg gtatacgaag ctggctcgtc ggtatggccg acgtaccgca tcgaaccgcc
     1501 ccgccgtcgg cgggggcgcc gcggccagac atggcagccc gatggcgatt atcgccacgg
     1561 cgagcgctga aggtctttta cagaaccatg cttcgagctc cgatagccta cagccggaca
     1621 agccgcccgc gccacggccc ggagccgacg ggccgagtcc gaaagggatc gacctcccgc
     1681 tatttggaaa ggagtatggc atgtacgatt ctcttatgta cggaagcgag ccctccccgg
     1741 gcttgcctcc caacgagccg gtgcgttcgt acgctcccgg ttctctcgag cgctcggcca
     1801 tcgacgccga gctcaaggcc atggccgcga atcctatcga gattcccctc gtcatcggcg
     1861 gcaaggaagt ccgcacgggg cggatcaagg agatccgggc gccgtacgac cgctcgctcg
     1921 tactcggccg ctaccacgag gccggcgagg ccgaggcgat cgcggcggcc gaggccgcgg
     1981 ccgtggccta ccgggagtgg tccgagacgc cgttccagga gcgggcggcg gtattccgca
     2041 aggctgccga cctgatctcc gccggctggt ggaaggcgct caacgcgtcg acgatgcttg
     2101 gccagtccaa gaacgcctac caagctgaga ttgactcgac ctgcgagacc gctgacttct
     2161 tccgtatcaa cccggccctg gccgagcggc tctacgccga gcagccgcgc tccggtccgg
     2221 gcgaacgcaa cctgagcgac tatcggccgc tggacggctt cgtctacgcc gtcacgccgt
     2281 tcaacttcac ggccatcgcc gcgaacctcc cgtcggcgcc ggccctgatg ggcaataccg
     2341 tggtctgg
//
LOCUS       DAOOLN010000193         2346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_56500_length_2346_cov_3.195548, whole genome shotgun sequence.
ACCESSION   DAOOLN010000193 DAOOLN010000000
VERSION     DAOOLN010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56500_length_2346_cov_3.195548"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1763)
                     /locus_tag="PLW80_03310"
     CDS             complement(<1..1763)
                     /locus_tag="PLW80_03310"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HPB65561.1"
                     /translation="MNEKVVDGVSKAAPVKQPGALSPRIRWLREYYFSGSTRAWNNQQ
                     IAFSCGVPWDVVYNECNYHIVPETYAFFQTFTSSYLQGAETVALRDGFWELSLPERRA
                     WFNKTVMLEYMPQEILPGDLLAGGRFNVHTSLCLDRGQAARRAKALYGKRGLRREAIG
                     FHNHGFGNAGPTAGHLIPDYERVLRHGFKAIDEELEGLYEALSYDERVGGRGAQLRAM
                     RTACSMPRELAARYRDEALKLAASEPDAARRAELERMAANLERVPWEGARDLWEAVQS
                     LWLTHMLVMSDENYPGPGVSFGNLDRILDPFWAVSKEAGMSRELAKEILECFWIHANT
                     AYDSMIKVGNQGITAGFGQLFNLSGLGPGGRDMTNDATWLLLEVIDDMSPMLEPKPNL
                     RLHRGSPDALLDRAVDMIADSQGAPFLLNFDERSMAGMMRESRLAGVGELIREDNVHE
                     YASVGCLENTMVGNDRSGTVDCNLNLLKAVELALGDGRPLVTFTDTITGVTERPLRQG
                     PRTGDPRKFDSFDRFWEAYAVQTRHIVDLIVRLYDRGDALRAEFGPTPYLSTLVRGCA
                     AKGRDVTRGGAELNFVTIEGVT"
BASE COUNT          390 a          807 c          757 g          392 t
ORIGIN      
        1 gtcacgccct ctatcgtcac gaagttcagc tccgcgccgc cccgcgtcac gtccctgccc
       61 ttggccgcgc agccgcgcac cagggtcgac aggtagggtg tcgggccgaa ttcggctctc
      121 agcgcgtcgc cacggtcgta gagccgcacg atcaggtcga cgatatgcct ggtctggacg
      181 gcgtacgcct cccagaaccg atcgaagcta tcgaatttcc tcgggtcgcc ggtacgaggc
      241 ccttgccgga ggggtcgttc cgtgacgccg gtaatcgtgt cggtgaacgt cacgaggggc
      301 cggccgtcgc cgagcgccag ctcgaccgcc ttgagcaggt tcaggttgca gtcgaccgtc
      361 cccgagcggt cgttcccgac catcgtgttc tccaggcagc cgaccgaggc gtactcgtgg
      421 acgttgtcct cgcgtatcag ctccccgacg ccggcgagcc gcgactcgcg catcatgccg
      481 gccatggagc gctcgtcgaa gttgagcagg aagggcgcgc cctggctatc cgctatcatg
      541 tcgaccgccc ggtcgagcag ggcgtcgggg ctgccgcgat gcaagcgcag attgggcttg
      601 ggctcgagca tcggcgacat gtcgtcgatc acctcgagga gcagccaggt cgcgtcgttc
      661 gtcatgtccc ggccgcccgg ccccaggccg gacaggttga acagctggcc gaaaccggcg
      721 gtgatgccct ggttgccaac cttgatcatc gagtcatagg ccgtgttggc gtgtatccag
      781 aagcactcga ggatctcctt ggccagctcc cggctcatgc ccgcctcctt tgaaacggcc
      841 cagaacgggt cgaggatgcg gtccaggttg ccgaaggaga cgcccggccc cggataattc
      901 tcgtcgctca tcacgagcat gtgcgtgagc cacagcgact gcacggcctc ccagaggtcg
      961 cgcgcgccct cccaggggac gcgctccagg ttggcggcca tacgctctag ctcggcgcgg
     1021 cgcgcggcgt ccggctcgga cgcggccagc ttgagcgcct cgtcgcggta gcgggccgcc
     1081 agctcccgag gcatcgagca ggccgtcctc atcgcgcgta gctgagcgcc gcgcccaccg
     1141 acccgctcgt cgtagctcag ggcttcgtac aggccctcga gctcctcgtc gatcgccttg
     1201 aagccgtgcc tgagtacgcg ttcgtagtcg ggtatcaggt gccccgccgt cggtcccgcg
     1261 ttcccgaagc cgtggttatg gaagcctatc gcctcgcgcc tgagcccgcg cttcccgtac
     1321 aaggccttag cgcgacgagc ggcctgaccg cggtcgaggc acagcgaggt atgcacgttg
     1381 aaccgtccgc cggccaggag gtcgccgggc aggatctcct ggggcatata ctcgagcatc
     1441 accgtcttgt tgaaccaggc gcggcgctcg ggtaggctca gctcccagaa gccgtcgcgg
     1501 agggccaccg tctcggcgcc ctgcaagtac gagctcgtga aggtctggaa gaaggcgtag
     1561 gtctccggga cgatgtggta attgcactca ttataaacca cgtcccaggg aacgccacag
     1621 gagaaggcga tctgctgatt gttccaggcc ctcgtcgagc ccgagaagta gtattcgcgt
     1681 agccatcgta tcctcggcga cagggcgccg ggctgcttaa ccggcgcggc cttgctgacg
     1741 ccatccacga ccttctcgtt catacgcgcc ccctcaatct ggttctacca attatcgtag
     1801 taccattcgg agatgcgcgc aagagaaatt ttccaccttc tatcacacga aggcgccaga
     1861 cacggaggaa gcggcatacg cgaagacgag gacaagatct caggatggaa gaagatgacc
     1921 atgatgaggg ccaaggttcg agcctccctc atcgtggtta tcggcaggag taacggccgc
     1981 ggccgttacg taacccgaac gtcaggagcg gcgcgccgct atggcgcgcc gccattgcgc
     2041 gctcctacgt aacctgagcg tcgggggcgg tgcgccgcta tggcgcgtcg ccacagcgcg
     2101 ttcccacgca acccgagccc cggggcggtg cgccgctatg gtgcgccgcc acagcgcgct
     2161 cctacgtaac ctgagcgtcg ggggcggtgc gccgccacag cgcgctccta cgtaacccga
     2221 gccccggggc ggtgcgccgc tatggcgcgc cgccacagcg cgctcccgcc gaacgtcgtg
     2281 ccggcgaccg agacccgccc cgctaccggt cgcgacgcgc cgccccgaaa accttgctca
     2341 taacca
//
LOCUS       DAOOLN010000194         2343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_56593_length_2343_cov_4.917832, whole genome shotgun sequence.
ACCESSION   DAOOLN010000194 DAOOLN010000000
VERSION     DAOOLN010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56593_length_2343_cov_4.917832"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PLW80_03315"
     CDS             complement(<1..508)
                     /locus_tag="PLW80_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPB65562.1"
                     /translation="MIGEASSKGGDPFIEYGRKHPGASASFNAPGRGGEPASMRISGL
                     IDSDTAEDFLEASLKALGTLSRGESINLDLKDVRFVSSTGVGTLTKLLAAAESLGVSL
                     RICAVSPACEDVFSILGLLDYFGIEPSAGSGAAQGGGRYEGRDGEAGASPSGGACPSP
                     SRRVARGPC"
     gene            527..1360
                     /locus_tag="PLW80_03320"
     CDS             527..1360
                     /locus_tag="PLW80_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPB65563.1"
                     /translation="MNGGRNPGLASESNFAMMERPSNRFVSRRLVPMLKSILVLSYFW
                     LSAAIASPFALIYILASALGLGRALRPALGVVTRAWSKSVLAAVGARTIVEGIENVPD
                     DEKLCFVANHQGDLDIVMMLAYMPRPVGFVAKKEAAWFPFINLWIAALGSAFIDRGSP
                     RQGKRAIDRGVRSVERGNAMVIYPEGTRSRGPSMLPFKKGAFKLATRPGATIVPVTID
                     GAYKVWELHGRIESADVRIVVHPPIRTAGLDAEARKELPSLVEATIASALPASSAAGP
                     V"
     gene            complement(1357..2232)
                     /locus_tag="PLW80_03325"
     CDS             complement(1357..2232)
                     /locus_tag="PLW80_03325"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPB65564.1"
                     /translation="MVTKDCFDEGDLRHFEPDGKVGLLATVDPDGLPHVTLITAMQAT
                     DTRRLIFGQFCEGRGKRYAEERRRVGFLFLSLDKRYWRGTADWTGKARSGPEHELMNK
                     KPMWRYNSYFGIHTVHYLDLVSTTAAASVDMGAVVAGAAKAALAGRRASLALGLEPHP
                     GAMNPWTRGLISRLGNLKFVSWIGDDGYPRIAPQLAATCLGGGRVYLPGAEHGDELAE
                     IRDGASVALFAMALSMEDVLVRGRIRRTGGRPGGLPAGRASGCLDVDWVYNSMPPVAE
                     RIYPPVRLAEKVTAF"
BASE COUNT          383 a          832 c          770 g          358 t
ORIGIN      
        1 gacagggtcc gcgtgctacc cgccgagagg gactaggaca agcccccccc gacggcgagg
       61 ccccggcctc gccgtcgcgc ccctcgtagc ggccgccgcc ctgagccgcc ccggacccgg
      121 ccgacggctc tataccgaag taatcgagca ggccgaggat ggagaacacg tcctcgcagg
      181 cgggcgatac ggcgcagatg cgtagcgaca cgccgagcga ctcggccgcc gcgagcaact
      241 tggtaagcgt ccccaccccc gtggacgaca cgaagcgtac gtccttgagg tcgaggttga
      301 tcgactcgcc ccgtgacagg gtcccgagcg ccttcagcga cgcctcgagg aaatcctcgg
      361 cggtatcgct gtctatcagg cccgagatgc gcatcgacgc cggctcaccg ccgcgcccgg
      421 gagcgttaaa gctcgccgag gcgccgggat gcttgcgccc gtactcgatg aatggatctc
      481 cgcccttaga ggaagcctcg ccgatcatga ggataagtat agtggcttga acggcggccg
      541 caaccccggc ttggcatcgg agtcgaattt cgcgatgatg gagcggccct cgaaccgttt
      601 cgtatcccgg aggttggtcc cgatgctcaa gtcgatactc gtgctgtcat acttctggct
      661 cagcgccgcg atagcttcgc cattcgcgct gatctacatc ctggcgagcg cgctcgggct
      721 cgggcgggcg ctcaggccgg ccctcggcgt ggtgacgcga gcctggtcca agagcgtcct
      781 ggcggccgta ggcgcccgca ccatcgtcga gggcatcgag aacgtgcccg acgacgagaa
      841 gctatgcttc gtcgccaacc accagggcga tctcgacatc gtgatgatgc tcgcgtacat
      901 gcccaggccg gtcggcttcg tcgcaaagaa agaggcggct tggttcccct tcatcaacct
      961 atggatagcg gccctcggct cggccttcat cgaccggggt agcccaaggc agggcaagcg
     1021 cgccatcgac cgcggcgtcc gtagcgtcga gcgaggcaac gcgatggtga tctaccccga
     1081 gggcacgagg agtcgcggcc cgtcgatgct cccgttcaag aagggcgcct tcaagctggc
     1141 tacgagaccg ggggcgacga tcgtaccggt gacgatagac ggcgcctaca aagtctggga
     1201 actccatggc cgcatcgagt cggcggacgt gcgcatcgtc gtccacccgc ccatccgcac
     1261 cgccggcctg gacgccgagg ccaggaaaga gctaccgtct ctggtcgagg cgacgatagc
     1321 ctcggcgcta cccgccagct cggccgctgg cccggtctag aaggcggtca ccttctcggc
     1381 gagcctgacc ggcgggtaga tacgctcggc gaccggcggc atcgagttat agacccagtc
     1441 gacgtcgagg cagccggagg ctcggcctgc cgggagtcct cccggccgcc cgcctgtcct
     1501 gcggatgcgg ccgcgcacga ggacgtcctc catgctgagc gccatcgcga acagcgccac
     1561 gctcgccccg tcccgtatct ccgccagctc gtcgccgtgc tccgcgcccg gcaggtagac
     1621 gcggccgccc cccaggcagg tcgccgccag ctggggcgcg atgcgcgggt agccgtcgtc
     1681 gcctatccac gagacgaact tgaggttgcc gagcctcgat ataagccccc tggtccaggg
     1741 attcatcgct ccgggatgcg gctcgagccc gagcgccaag gatgcccgcc gaccggccag
     1801 ggcggccttg gccgctccgg cgacgacggc gcccatgtcc accgaggccg cggccgtcgt
     1861 cgagaccagg tccaggtagt gcacggtatg gatgccgaag tacgagttgt agcgccacat
     1921 cggcttcttg ttcatcagct cgtgctccgg ccccgagcgg gccttgcccg tccagtcggc
     1981 cgtaccgcgc cagtagcgct tgtcgagcga caggaaaagg aagccgaccc tgcgccgctc
     2041 ctcggcgtag cgcttgccgc ggccctcgca gaactggccg aagatcaggc gccgcgtatc
     2101 ggtcgcctgc atcgcggtga tcagcgtcac gtgcggcagg ccgtccgggt caacggtcgc
     2161 caggaggccg accttgccgt ccggctcgaa atgccgcagg tcgccctcgt cgaaacaatc
     2221 cttggtaacc atcgatgcct ccccccggcc ggtagccggt ccctacgatt cgcgcctcgt
     2281 catgccggtc cagcgcaccc ggtcggccgg ccagccttcg gccacggcca ggcctaccag
     2341 ggc
//
LOCUS       DAOOLN010000195         2337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_56812_length_2337_cov_2.798422, whole genome shotgun sequence.
ACCESSION   DAOOLN010000195 DAOOLN010000000
VERSION     DAOOLN010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56812_length_2337_cov_2.798422"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="PLW80_03330"
     CDS             complement(<1..979)
                     /locus_tag="PLW80_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65565.1"
                     /translation="MKRHNIALTLGLAALVALASASSWEGSAMMGAYGDFPSSGYYAA
                     CNSFARNTSIDVVNLENGRSVTVIVTRGLESSGVFMMLSVEAAEALGLQRGGVTRVRA
                     SEPRSAVELAPSAGTSASMDPDINPRLLAAAELRRLGYELAPAAPSTSRQPAVSAVRV
                     QPEAPSRTVPAVAEPPAPAVSAPAIPVPPTAVAEARPQAAAPAVAAAPPHVAAPSAIG
                     REAPAVAPASRVAIAEPKPIGKETPESLASSKPRPVRTIVLPQLPEPEGPTTKAAVAA
                     PVATPQSAPEAPAATAPEAVAMSLPVPDETPLRVAVGATPKRYESPAARP"
     gene            complement(966..1484)
                     /locus_tag="PLW80_03335"
     CDS             complement(966..1484)
                     /locus_tag="PLW80_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65566.1"
                     /translation="MRRLAIAAFFALAAAAPRLVAQTIEVSVMERGVEPDGPASLSSE
                     HVIAGMLDGLFESGFIATNARPAPGDDASFEAFSPGADAVEGFVDFVILVLAEYAPET
                     VVPACSYRILRVPDGAEVSRGKVPAATPVSKASIDIEKACMAVGSAISAACGGALRSL
                     SVSRGDDGYEEA"
     gene            1639..2199
                     /gene="loaP"
                     /locus_tag="PLW80_03340"
     CDS             1639..2199
                     /gene="loaP"
                     /locus_tag="PLW80_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator LoaP"
                     /protein_id="HPB65567.1"
                     /translation="MEYFAVQVWTGKEDEFADALSADPRVEAKAMVPKRALEIRRAGK
                     VRREERPLFPGYVFIATDADRLSDDQRWLLRGSRNFVRALPNNAEPRPVKEKDRRLLA
                     HFMSFGKVADISKVRFDADDRIVVVEGPLKGLEGLIVRVDARKRRAKIRLDMCENSFL
                     VDMGFEILDRAAKGTEGNDGDESRQP"
BASE COUNT          401 a          756 c          823 g          357 t
ORIGIN      
        1 ccggcctggc ggccggcgat tcgtagcgct tcggggtagc gccgaccgcg acgcgtagcg
       61 gcgtctcgtc cggcacgggc agcgacatcg ccaccgcctc cggagcggtc gcggcaggag
      121 cctcgggagc cgactgtggc gtagccacgg gcgccgcgac ggctgccttg gttgtcggcc
      181 cttctggctc gggcagctga ggcaacacga tcgtacggac cggcctcggc ttcgacgagg
      241 cgagcgattc gggcgtttcc ttgccgatcg gcttaggttc ggctatggcc acacggctgg
      301 cgggagccac ggcgggagcc tcgcggccaa tggccgaagg cgcggctacg tgaggcggag
      361 ccgcggcgac ggccggggcc gcggcttgag gccgagcctc tgctacggcg gtaggcggaa
      421 ccgggatcgc cggagcagag actgccgggg cggggggttc ggcgacggct ggaacggttc
      481 tagacggggc ctcgggctgg acgcggacgg cggataccgc cggctgcctg gaggtggacg
      541 gggccgcggg agccagctcg tagccgaggc gcctgagctc ggcggccgcc aggagacgcg
      601 gattgatgtc cggatccatc gaagcgctcg tcccggcgga gggggcgagc tcgacggcgc
      661 tccgcggctc cgaggccctg accctcgtca cgccgccgcg ctgcaggccg agcgcctccg
      721 cggcctcgac ggacagcatc atgaagacgc cggagctctc gaggccgcgc gtcacgatca
      781 ccgtgaccga gcgtccgttc tcgagattaa ccacatcgat ggaggtatta cgcgcgaagg
      841 agttgcacgc ggcgtagtag cccgacgaag ggaaatcccc gtaggctccc atcatcgcgc
      901 tgccttccca tgacgaagcc gacgcgaggg cgacgagcgc cgccaggccg agcgtcaacg
      961 cgatattatg cctcttcata tccgtcatcc ccccgcgaaa cgctaaggct ccgcaacgcg
     1021 ccgccgcagg cggccgaaat ggccgatccg acggccatgc acgctttctc tatatcgatg
     1081 ctcgccttgg agacgggcgt agccgccgga accttccctc gcgatacctc ggcgccgtcg
     1141 ggtaccctga gtatcctgta gctgcaggcc ggcacgaccg tttcaggcgc gtactcggcc
     1201 agtaccagga tgacgaagtc gacgaacccc tcgacagcgt cggctcccgg agagaacgcc
     1261 tcgaacgacg cgtcgtcgcc cggggccggc ctggcgttcg tagcgataaa accggactcg
     1321 aataggccgt cgagcatacc ggcgatgacg tgctcggagg atagcgacgc cggtccgtcg
     1381 ggttcgacgc cccgctccat aacggagacc tcgatggtct gcgcgaccag gcgaggggcg
     1441 gccgccgcca gggcgaaaaa agccgcaatc gcgagtcttc tcatcgttcg catccttttc
     1501 ttcttggaat atcggccaga cggacgacat gatttaggcg tatcgtcgat acggggctca
     1561 gccttgtcgg ccctctcccc ttcgtgctag cctcgggagg gttcgttcat cgggcggaac
     1621 ggcgatcacg gaggagcgat ggagtatttc gccgtacagg tatggaccgg caaagaggat
     1681 gagttcgccg acgcgctatc ggccgatccg cgcgtcgagg ccaaggctat ggtgcccaag
     1741 cgggccctcg agatacggcg ggcgggcaag gtacggcgcg aggagcgccc cctcttcccg
     1801 ggctacgtct tcatcgcgac cgacgccgat cgcctgagcg acgaccagcg ctggttattg
     1861 cgtggctcga ggaacttcgt acgggcgctc ccgaacaacg ccgagccgag gccggtcaag
     1921 gagaaggacc ggaggctact cgcccacttc atgtcgttcg ggaaagttgc cgatatatcg
     1981 aaggtacgtt tcgacgcgga cgatcgcatc gtcgtcgtcg agggcccgct caaggggctc
     2041 gagggcctga tcgtcagggt cgacgcgcgc aagcgccggg ccaagatacg cctggacatg
     2101 tgcgagaact cgttcctcgt cgacatgggt ttcgagatac tcgaccgggc ggcgaaagga
     2161 accgagggca acgatggaga tgaatcccga caaccctagg atatacatca tcggcgccgg
     2221 cttcgccggg cgcgcgatcg cgcgggagat ccgggccaag gcggtcctcg gcaccatcgt
     2281 cgccttcctc gacgacgacg agggcaagct gggcaggcgc gtcgacggcg tgccagt
//
LOCUS       DAOOLN010000196         2327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_57151_length_2327_cov_3.163732, whole genome shotgun sequence.
ACCESSION   DAOOLN010000196 DAOOLN010000000
VERSION     DAOOLN010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57151_length_2327_cov_3.163732"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PLW80_03345"
     CDS             complement(<1..307)
                     /locus_tag="PLW80_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65568.1"
                     /translation="MRKVAFALLASLDFSSMAGAQGRNIVILHSYQYDYEWTRLEHAG
                     IMSAFEDSGEGALIIKTDYLDAKRSWNPAKARNAADYLRDTYRDYSAVRVNPTTAPTI
                     "
     gene            complement(312..1238)
                     /locus_tag="PLW80_03350"
     CDS             complement(312..1238)
                     /locus_tag="PLW80_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65569.1"
                     /translation="MDGSTTGLPGGSLAFAKLCGAGNDFIVLDGEAARSAPGAETIRL
                     LCDRRYGVGADGLMLLRRGSGAGAGGDDAAFEVDFYNADGSGGMLCGNGVRCALERAR
                     ILGWIEPDRAVGFRFAGRDYRGSSLGEGMARFELDPRVRMDAPRLVRVGDLSLSGRFV
                     DVGSPHFVVDVAAILDGDGRRAFGSLDDVPLDALGQAVRNHPDFGAAGVNANVTELLG
                     ATLFVRTFERGVEAETLSCGTGSASSALVRWRDGELRPPVTVVARSGDELAVDFEAAS
                     DGSLTRLSLSGPAIQTFEGRVVALPLYGQAVY"
     gene            1299..2318
                     /locus_tag="PLW80_03355"
     CDS             1299..2318
                     /locus_tag="PLW80_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HPB65570.1"
                     /translation="MKLRVKPTILRAAILLILIAVQLALMAATIWTTSQASALLNGVL
                     SLVSLLVVLYIVRSNADPSYKLTWSVLILIVPVFGGLFYLFFGLQPSSRKLRRRIAGL
                     DISARPYRRQEGGVVEAIGAMRDDFGRQAAYLSRTAGFPAYDGSSAEYLTSGEAKFEQ
                     LKAELAKAERFIFLEYFIIEAGLMWDSILAILEEKAAAGVEVRVLYDDIGCLLTLPSG
                     YAESLRRKGIHCAAFNPAQGYPSAILNHRDHRKIVVIDGRVAFTGGVNLADEYINEVE
                     KYGHWKDAAVVLRGKAVDGFTLMFLGMWNLETNESDDYPRYLAGRATSSPADAGAASE
                     TECDG"
BASE COUNT          417 a          763 c          771 g          376 t
ORIGIN      
        1 ggatggtcgg tgcggtggtc gggttgacgc ggaccgccga gtagtcgcgg tacgtatcgc
       61 gaaggtagtc ggccgcgtta cgcgccttgg cgggattcca cgatcgcttg gcgtcgaggt
      121 agtccgtctt gatgatcaga gcgccttcgc cggaatcttc gaaggccgac atgatgccgg
      181 cgtgctcgag gcgcgtccat tcgtaatcgt actgatagga atgcagtatg acgatattcc
      241 tgccctgggc tccggccatc gaagaaaagt ctagggatgc gagcagggcg aacgcgactt
      301 ttctcatgcc cctagtatac agcctgcccg tataacggca aggctaccac gcgtccctcg
      361 aaggtctgaa tcgccggacc ggagagcgac agcctggtca gcgagccgtc cgacgcggct
      421 tcgaagtcga cggcgagctc gtcgccgctc cgcgctacca cggtgacggg cggccgtagc
      481 tcgccgtcgc gccagcggac gagcgcgctg gaagcggaac cggtgccgca cgacagggtt
      541 tcggcctcga cgccgcgctc gaaggtacgg acgaacagcg tagctccgag gagctcggta
      601 acgttggcgt tgacgccggc ggcgccgaaa tcggggtggt tccgcacggc ttggcccagt
      661 gcgtcgagcg gtacatcatc gaggctgccg aaggcgcggc ggccgtcgcc gtccagaatg
      721 gccgcaacgt cgacgacgaa gtgcggactg cccacatcga cgaagcggcc gctcagggac
      781 aggtcgccga cgcgcacgag gcgcggggcg tccatgcgga cgcgagggtc gagctcgaag
      841 cgggccatcc cctcgccgag ggaggagcct ctgtagtcgc ggccggcgaa gcggaagccg
      901 acggcccgat cgggctcgat ccagcctagg atcctggccc gctccagggc gcagcgtacg
      961 ccgttaccgc agagcatgcc gcccgagccg tcggcgttgt agaagtcgac ctcgaaggcc
     1021 gcatcgtcgc ctccggcgcc ggcgcccgag ccgcgccgga ggagcatgag gccgtcggcg
     1081 ccgacgccat agcggcggtc gcagaggagc cgtattgtct cggcccccgg cgctgacctc
     1141 gccgcctcgc cgtcgagaac gatgaagtcg ttaccggccc cgcatagctt ggcgaaagcc
     1201 agagagcccc cagggagccc cgtcgtcgat ccgtccatgg acagggagta tagcacccaa
     1261 gcttcggaac acgatacaat ccaggcggag aacgacaggt gaaactacgc gtaaagccga
     1321 ccatactgcg ggccgccata ctcttgatcc tgattgcggt tcagttggcg ctgatggcgg
     1381 cgacgatctg gacgacgagc caggcctccg cgctactcaa cggcgttcta tcgctcgtca
     1441 gcctgctcgt ggtgctgtac atcgtccgct ccaacgccga tccttcctac aagctcacct
     1501 ggtcggtact catcctgatc gtgccggtct tcgggggcct cttctacctc ttcttcgggt
     1561 tacagccctc gtcgcgcaag ctgcgcaggc gcatcgccgg actggacatc tcggccaggc
     1621 cgtataggcg ccaggagggc ggcgtcgtcg aggccatagg cgcgatgcgc gacgacttcg
     1681 gcagacaggc cgcctacctg agccgaacgg ccggtttccc ggcctacgat ggctcctcgg
     1741 ccgagtacct gacctccggt gaggccaagt tcgagcagct caaggccgag ctggccaagg
     1801 ccgagcgctt catcttcctc gagtacttca ttatcgaagc cggcctcatg tgggactcga
     1861 tcctcgcgat actcgaggaa aaggctgccg ccggcgtcga ggtgcgagtg ctctacgacg
     1921 acatcggctg cctcctgacc ctgccgtccg gttacgccga gagcctgcgc cgcaagggca
     1981 tccactgcgc cgcgttcaac ccggcccagg gctatccatc ggccatcctg aaccaccgcg
     2041 accaccgcaa gatcgtcgtg atcgacggcc gcgtcgcgtt taccggcggc gtcaacctgg
     2101 cagacgaata tatcaatgag gtagaaaagt acgggcactg gaaagacgcc gccgtcgtcc
     2161 tgcgcggcaa ggccgtggac ggcttcacgc tcatgttcct ggggatgtgg aacctggaga
     2221 cgaacgaatc ggacgactac cctcgctacc tggcggggcg cgccacctcg tcaccggccg
     2281 acgcgggggc cgcgagcgag actgaatgcg acggctaagc ctgtaga
//
LOCUS       DAOOLN010000197         2327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_57155_length_2327_cov_3.109595, whole genome shotgun sequence.
ACCESSION   DAOOLN010000197 DAOOLN010000000
VERSION     DAOOLN010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57155_length_2327_cov_3.109595"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PLW80_03360"
     CDS             <1..181
                     /locus_tag="PLW80_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65571.1"
                     /translation="VIIPGFASHVAQATLFKAIPCDAAPKGAVGFERGANALKNAMER
                     AGGRCKRETGGANGS"
     gene            complement(302..964)
                     /locus_tag="PLW80_03365"
     CDS             complement(302..964)
                     /locus_tag="PLW80_03365"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPB65572.1"
                     /translation="MNAMPASPIGTSSIFGDADGAGVWSRCLDAAIKAFNSGSLGIAA
                     AVVDPGMAIVSLGRNRMDEIPDDPLSISGSSVSHAETNAIHGLGRRSLGDYELSLYTT
                     VEPCPMCVGAIGMSRIRRVVVASRDPHAGSLDLLESSGYLRSKGITTEEPGGAIERVF
                     FGIHYLSIKRLPHIAPDHRIFAAFQTRYGGLMEEIDDRLGTVPLATLRLTYEAIDRLF
                     GP"
     gene            complement(978..1925)
                     /locus_tag="PLW80_03370"
     CDS             complement(978..1925)
                     /locus_tag="PLW80_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65573.1"
                     /translation="MTILLIRPLRSARALALAARALALIALAGLMVVPAAALDLEASA
                     FFGNLGLPWAGEEPLTGAQFPSDAWIYGGKAGFTDELGEGLSLYAGYETDPTLRHVVK
                     GIISYESGIASISAGPRVGVFNTAQTPLKAGIEVGFRLDAPGIAFFSVDTASSMGVGL
                     ASAGDYSQEYSDISAGWYVYNAICSFSMTTKRYTRVLASGEPLVDASNDYLFSVDTYK
                     KGAPYRVLTELGYRTMTRSYPDGSSDGLGAVVLAAEISADVSPALTLVAGLDSGVYVF
                     GIESLAGRGPASSAFMFSASLGAVVRLPQAGAAIEQAAE"
     gene            complement(1945..>2327)
                     /locus_tag="PLW80_03375"
     CDS             complement(1945..>2327)
                     /locus_tag="PLW80_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65574.1"
                     /translation="EAFRMDLLYFMFEPRVRFDVGELVLSLFFHPAWYLQEETDESGA
                     LEFRFDLGFGEASEGSFKGGVESELAYNPNLDQNTLTMEVAPYLQTLRNGVRWDARLA
                     VRVFPFPSPWYGMFMPTIAVSTAF"
BASE COUNT          410 a          734 c          805 g          378 t
ORIGIN      
        1 ggtgatcatc ccggggttcg catcgcacgt cgcgcaagcg acgcttttca aagcaattcc
       61 ttgcgatgcc gcgccgaaag gcgcggtagg gttcgagcgc ggcgcgaatg cgctgaaaaa
      121 cgcgatggag cgtgcaggag ggcgctgtaa gcgcgagact ggaggcgcga acggatcgta
      181 gggaggggga taccccctcg agcggcggta taccgccgct ctcacccctg cgatcctgcg
      241 caccgatatg gtatagctgc ggtgcgtacc agctgtaacc gacccataat cttcataaca
      301 ctcaagggcc gaacagcctg tcgatcgctt cataggtcag tcggagcgtc gccaacggca
      361 cggttccgag cctgtcgtcg atctcttcca tgagcccgcc gtatcgggtc tggaaagcgg
      421 cgaagattcg atggtcgggc gcgatatggg gcaggcgctt gatggatagg taatggatgc
      481 cgaagaacac ccgctcgata gctccgcccg gctcttcggt cgtgatgccc ttggaacgca
      541 ggtagccgct cgactcgagc aggtcgaggc tgccggcgtg ggggtcgcgc gaggcgacga
      601 cgacgcggcg tatcctggac atgccgatgg cgccgacgca catcgggcag ggctcgaccg
      661 tggtgtagag ggacagctcg tagtcgccga ggctcctgcg gccgaggccg tggatggcgt
      721 tggtctcggc gtgcgagacg gagctgccgc ttatggagag cggatcgtcg ggaatctcgt
      781 ccatccggtt ccgcccgagg ctgacgatgg ccattcccgg atcgacgacg gccgccgcta
      841 tgccgagcga tcctgagttg aacgctttga tcgcggcgtc gaggcaccgg ctccatacgc
      901 cggcgccgtc ggcgtcgccg aagatcgacg acgtccctat cggagacgcg ggcatcgcgt
      961 tcataacgag actggcgcta ctcggcggcc tgctcgatag ccgcgccggc ctgcggcagc
     1021 cggaccacgg cgccgaggct cgcgctgaac atgaacgccg acgaggccgg tccgcggccg
     1081 gccaggctct cgatgccgaa tacgtagacg cccgagtcga ggccggcgac gagggtcagc
     1141 gccggcgata cgtccgcgct gatctcggcc gccagcacga ccgcgcccag gccgtccgag
     1201 ctcccgtccg ggtaggaccg ggtcatggtg cggtagccga gctcggtgag cacccggtac
     1261 ggggcgcctt tcttgtaggt atcgaccgag aagaggtagt cgttcgacgc gtcgacgagc
     1321 ggctcgccgg aggcgagcac gcgggtgtag cgcttggtcg tcatcgagaa ggaacagatg
     1381 gcgttgtaga cgtaccagcc ggccgatatg tcggaatact cctgcgagta gtcgcccgcc
     1441 gacgccagcc ccacgcccat cgacgaggcg gtgtcgaccg agaagaaggc tatgcccggc
     1501 gcgtcgagcc tgaagccgac ctcgatgccg gccttgagcg gcgtctgggc cgtgttgaac
     1561 acgccgacgc gcgggcccgc gctgatgctc gcgatgcccg actcgtagct gatgatgccc
     1621 ttgacgacgt gcctgagcgt cgggtcggtc tcgtagccgg cgtacaggga cagcccctcg
     1681 ccgagctcgt cggtgaagcc ggctttgccg ccgtagatcc atgcgtcgct cgggaactgg
     1741 gcgccggtca acggctcctc accggcccac ggcagtccca gattgccgaa gaaggcgctc
     1801 gcctcgaggt cgagggccgc ggccggcacg accatgaggc cagctagcgc tatcagagcc
     1861 agcgctcgcg cagccagagc cagcgctcgc gcagatcgta acggacgtat cagcagtatc
     1921 gtcatcgcgt ggctccttgc tgtcctagaa ggcggtcgag acggctatgg tcggcatgaa
     1981 catgccgtac catgggctcg ggaacgggaa gacccgtacg gccagcctcg cgtcccaacg
     2041 gacgccgttc ctgagcgtct ggagatatgg cgctacctcc atcgtcagcg tgttctggtc
     2101 gaggttcggg ttataggcca gctcagactc gacgccgccc ttgaacgagc cttccgaggc
     2161 ctcgccgaag cccaggtcga agcggaactc gagcgctccg gattcgtcgg tctcctcctg
     2221 gaggtaccag gctgggtgga agaacaggct gagcacgagc tcgccgacgt cgaagcgtac
     2281 ccgcggctcg aacatgaagt agaggagatc catcctgaag gcctcgg
//
LOCUS       DAOOLN010000198         2326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_57181_length_2326_cov_3.502422, whole genome shotgun sequence.
ACCESSION   DAOOLN010000198 DAOOLN010000000
VERSION     DAOOLN010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57181_length_2326_cov_3.502422"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1861)
                     /locus_tag="PLW80_03380"
     CDS             complement(<1..1861)
                     /locus_tag="PLW80_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65575.1"
                     /translation="MLIWSDIERALTPSREDTALAADLAAAIDRGLPLAITQSVLIVA
                     SDAAMASLYATLEARSSPPEPRRLRSDSSWMLESDFCWINVRACASGSLPGTFIRAAK
                     LLPAIASDAILLAPFHPTQFDLCYAPETMTMADPTLFDRELVGAGVSAENQLRAFVSA
                     CHLLGKCVGYELLPYAAQFSRIAMERPQLFRWVALDDERRGLAHADPAFPYRSEDRLR
                     DAEQVSDIVATVKEDYGIRSFRVIEGDAPEVVDAKVKAYYSAIRLCADRGLWPVPAHA
                     RAGVGVPSFLRCDSSDDFPIFSYRDIDGADIGEDAYSVVAPFAFYDDMPPNSIPTEPV
                     RRNDEAIGYYAEVFSYWRDSFGFDFVRYNAADRVFSESVDDEGSVPSCDRPTSEVVAQ
                     AIRASRDGAPGVGAISPSKGDETGAFGALGFDLLMGGEALRRVDAPLLRDAFSLYDSL
                     AAERAGGARTSVCFAVDLPESSAPRLWGSPLRRVMGPERMRLRHVAARFLSVGAGRRP
                     MFETMGFQDGSTGLYEAGLSVRGLDWADDAAFAAGYAAIERFYVGLRPFLASAAIIDR
                     SVDDGLAWWQIRAGDSARLVVVALSLETAEGTAPGRIAVPIAKALGELSGAAYR"
     gene            complement(1868..>2326)
                     /locus_tag="PLW80_03385"
     CDS             complement(1868..>2326)
                     /locus_tag="PLW80_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB65576.1"
                     /translation="ETFGHRKAPDLVLSLALLLHDTGKPDAVPAGGRRFDGHSELGER
                     AARRLLGRLGYPGDVVDSVAFLVRYHMLPAALPRIPPSSVGRALDSPLFPTLLELYRC
                     DELSTFRGPDGYYEACAAYKAYLKNARNPYRDDDGKRRGDSLHRARPRTL"
BASE COUNT          389 a          768 c          834 g          335 t
ORIGIN      
        1 ggcgataggc cgcgccgctc agctcgccga gcgcctttgc tatcgggacg gcgatcctgc
       61 cgggcgcggt accttccgcc gtctcgagcg acagggcgac gacgacgagc ctggcgctgt
      121 caccggcgcg tatctgccac caggccaggc cgtcgtcgac ggagcgatcg attatggcgg
      181 ccgaggccag gaacggccgc aggccgacgt agaagcgctc gatggcggcg tagccggcgg
      241 cgaaggccgc gtcgtcggcc cagtcgagcc cgcgtacgga cagtccggcc tcgtagagtc
      301 cggtcgagcc gtcctggaag cccatcgtct cgaacatcgg ccgcctgccc gccccgacgg
      361 acaggaagcg ggccgcgacg tgccgtagcc gcatcctttc gggccccatg acgcgtcgta
      421 ggggcgagcc ccagagcctt ggggcggatg actcgggcag gtcgaccgcg aagcataccg
      481 aggttcgcgc tccgccggcc ctctcggccg ccagcgagtc gtacagcgag aacgcgtcgc
      541 gcaggagcgg cgcgtcgacg cgccgcagcg cctcgccgcc catcagcagg tcgaagccga
      601 gagcgccgaa ggccccagtc tcgtcgccct tgctcggcga gatggccccg acgcctggcg
      661 ctccgtccct ggacgcgcgg atggcctggg ccacgacctc ggaggtcggg cggtcgcagg
      721 acggcaccga tccctcgtcg tcgacgctct cggagaatac gcgatcggcg gcgttgtacc
      781 tgacgaaatc gaagccgaag gaatcgcgcc agtaggagaa gacctcggcg tagtatccga
      841 tagcctcgtc gttacggcgg accggctcgg tcgggatgga gttcggcggc atatcgtcat
      901 agaacgcgaa cggcgcgacg acgctatagg cgtcctctcc aatatcagcg ccgtcgatgt
      961 cgcggtacga gaagatcggg aagtcgtcgg acgagtcgca gcgtaggaac gacggaacgc
     1021 cgacgccggc ccgcgcgtgg gcaggcacgg gccagaggcc tctgtcggcg caaagcctga
     1081 tcgccgagta ataggccttg accttggcgt cgacgacctc gggggcgtcg ccctcgatca
     1141 cacggaagct acggatgccg taatcctcct taaccgtcgc cactatgtcg gagacctgct
     1201 cggcgtcgcg gagcctgtcc tcagagcggt acgggaacgc ggggtcggcg tgagccaagc
     1261 cccgccgctc gtcgtcgagc gcgacccagc ggaacagctg gggccgttcc atcgcgatgc
     1321 ggctgaactg ggcggcgtac ggcagaagct cgtacccgac gcacttgccg agcagatggc
     1381 aagccgacac gaaggcgcgg agctgattct ccgcgctgac ccccgcgccg acgagctccc
     1441 ggtcgaacag ggtcgggtcg gccatcgtca tcgtctcggg cgcgtagcac aggtcgaact
     1501 gggtcgggtg aaagggcgct agcaggatgg cgtcggaggc tatagccggt agcagcttgg
     1561 cggccctgat gaaggttccg ggcaagcttc cggacgcgca ggcgcgtacg ttgatccaac
     1621 agaagtcgga ctcgagcatc caggatgagt cgctacgcag cctacgcggc tcgggaggag
     1681 aactcctggc ctcgagcgtc gcgtacagcg acgccatggc cgcgtcgctc gctacgatca
     1741 atacgctctg cgtgatcgcg agcggcaggc cgcggtcgat ggcggccgcc aaatcggcgg
     1801 ccagcgccgt atcttcgcgg gagggcgtca gcgccctctc gatatcgctc catatcagca
     1861 tacggcacta tagcgtccgg gggcgcgccc tgtgaagcga gtcgccgcgg cgcttgccgt
     1921 cgtcgtcgcg gtacggattc ctggcgttct taaggtaggc cttgtacgcg gcgcaggcct
     1981 cgtagtagcc gtctggcccc ctgaaggtcg acagctcgtc gcaacggtac agctcgagga
     2041 gcgtcgggaa cagcgggctg tcgagcgccc taccgacgct cgacgggggg atgcgcggca
     2101 gggcggcggg cagcatgtgg tagcgtacga ggaaggcgac cgagtcgaca acgtcgcccg
     2161 ggtagccgag ccgcccgagc agccgcctgg cggcccgctc gcccagctcg gaatgcccgt
     2221 cgaaacggcg accgccggcc ggtacggcat cgggtttccc ggtatcgtgg agtagcaggg
     2281 cgagcgacag cacgaggtcg ggcgccttgc ggtggccgaa ggtctc
//
LOCUS       DAOOLN010000199         2310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_57806_length_2310_cov_3.046563, whole genome shotgun sequence.
ACCESSION   DAOOLN010000199 DAOOLN010000000
VERSION     DAOOLN010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57806_length_2310_cov_3.046563"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PLW80_03390"
     CDS             complement(<1..707)
                     /locus_tag="PLW80_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPB65577.1"
                     /translation="MRLVDPECAAIAAAFSRRDLRARLPESIAAKDPMYRRSGSSYDL
                     KSIREYQPFDDPRAIDWKLYGRSDRAFVKEFYDEADDEVAFLVDSSASLSCAPVEEYL
                     RFVSSMAFLLLSLGMSVRLWTFGSRLSERCVAARSRDSHGSIAGALASMRFERPTACS
                     RSFAQWRAGSRQRRAFVFSDFHEPNPTFRSSGGLFLIRYRTPFSSLALPGTEAEVRDP
                     ESGAVVVAPWGKADERA"
     gene            complement(704..1711)
                     /locus_tag="PLW80_03395"
     CDS             complement(704..1711)
                     /locus_tag="PLW80_03395"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB65578.1"
                     /translation="MQPDEFERSLASIKDLYDDYTAFVGTRVLGYPRILERLFIAFLS
                     GGNVLLEGAPGLGKTTLAKTWSEFFGLGFSRVQFTPDLMPLDIIGSNLLEEGPSGRSF
                     RFYRGPIFSNVVLGDEINRATPKTQSAFLEAMEEKRVTFLGEVHPLPDPFFVIATQNP
                     SELEGTYPLPKAQIDRFFMKLNFAMPDLHALLGIMDLQATEKHGRAIGAEASQEAIAG
                     WRALAPHIAAPEGVRAYIARIVEHSHPDRSPVELVRKYVLYGASPRSAIALLAAARTK
                     AAMAGRPSVSFDDVDELLADVAAHRLILNFEAEADGVTVADVLGAVRADAAKAFARER
                     R"
     gene            complement(1698..>2310)
                     /locus_tag="PLW80_03400"
     CDS             complement(1698..>2310)
                     /locus_tag="PLW80_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65579.1"
                     /translation="ILAASATTLRTLAAIAAGAALWYAMRLCQGALHPVLGRVPEWAA
                     LAANAGVEEALRLGLALLIARYVRRAGLEPGTVGLGVVAACSLGALENASYLAAFPTL
                     DAYWRLGYAVPIHAGAAALYAIGTGESENPDGASRPTSWPAGIAAAFTLAWAWHSGFN
                     IVAALFPFPALPIVGTVVNLIALSALVVALAIRYGYWSVYAAR"
BASE COUNT          388 a          748 c          831 g          343 t
ORIGIN      
        1 caggcccgtt cgtcggcctt gccccaaggc gccacgacca cggcgcccga ctcgggatcg
       61 cgcacctcgg cctcggtccc cggcagggcc aggctcgaga acggcgtccg atagcgtatc
      121 aggaacagtc cgcccgacga cctgaacgtc gggttgggct cgtggaagtc ggagaacacg
      181 aacgcgcggc gctgcctgga tccggcgcgc cactgggcga acgacctcga gcaggcggtc
      241 ggacgctcga agcgcatgga cgccagcgcc ccggctatcg agccgtgcga gtccctggac
      301 cgcgccgcga cgcaacgctc ggacagccgc gagccgaagg tccacaggcg aacgctcatg
      361 ccgagcgaca ggagcaggaa ggccatcgac gatacgaagc gtaggtactc ctctaccggg
      421 gcgcacgaca ggctggccga agagtcgacg aggaaggcca cctcgtcgtc cgcctcgtcg
      481 tagaattcct tgacgaaagc gcggtcggat cggccgtata gcttccagtc gatggcccgc
      541 gggtcgtcga aaggctggta ctcacggatg ctcttgaggt cgtagctcga gccggagcgg
      601 cggtacatgg ggtccttggc cgcgatcgac tcgggtagcc tggcgcgcag gtcgcgcctg
      661 gagaaggccg ccgcgatggc cgcgcattcc gggtcgacga gcctcatcgg cgctccctgg
      721 cgaaggcctt ggcggcgtcg gccctgaccg ctccgagcac gtcggccacc gtgacgccgt
      781 cggcctctgc ctcgaagttg aggatcaggc ggtgggcggc cacatcggcg agcagttcgt
      841 cgacgtcgtc gaacgaaacg ctcgggcggc cggccatcgc ggccttggtc ctggccgccg
      901 cgaggagggc tatggccgac ctcggcgagg cgccatacag gacgtacttc cgtaccagct
      961 cgaccggcga gcggtcgggg tggctgtgtt cgacgatgcg ggctatgtag gcgcgtacgc
     1021 cttcgggcgc ggctatatgg ggcgccaggg cccgccagcc cgcgatggcc tcctgcgagg
     1081 cctcggcgcc gatggcccgg ccatgcttct cggtcgcctg gagatccatg atgccgagca
     1141 gggcgtggag gtcgggcatc gcgaagttga gcttcatgaa gaaacggtct atctgcgcct
     1201 tggggagcgg gtaggtgccc tcgagctcgc tggggttctg cgtcgctatg acgaagaagg
     1261 gatcgggcag gggatggacc tcgccgagga aggtgacgcg cttttcctcc atcgcttcga
     1321 ggaaggccga ctgggtcttg ggggtcgccc ggtttatctc gtcgccgagc accacgttgg
     1381 agaagatggg ccctcggtag aagcggaacg aacgcccgga ggggccttcc tcgagcaggt
     1441 tcgagccgat gatgtcgagc ggcatcaggt ccggggtgaa ctggacccga ctgaagccga
     1501 gcccgaagaa ctccgaccag gtcttggcca gcgtcgtctt gccgagtcca ggagctccct
     1561 cgagcaggac gttgccgccg gataggaacg ctatgaacag gcgctcgagt atcctcgggt
     1621 agccgagcac gcgggtgccg acgaaggccg tgtagtcgtc gtagaggtcc ttgatcgagg
     1681 cgagcgatcg ttcgaattca tcgggctgca tacacgctcc agtatccgta ccgaattgcc
     1741 agcgccacga cgagcgccga gagggctatc aggttgacga ccgtgccgac gatcggcagg
     1801 gccgggaagg ggaacagggc cgcgactata ttgaagcctg aatgccaggc ccacgcgagc
     1861 gtgaaggccg cagctatccc ggccggccag ctcgtcggac gactcgctcc atcggggttt
     1921 tcgctctccc cggtacctat ggcgtacagc gccgcagccc cggcgtggat cggcacggcg
     1981 tagcccagac gccagtacgc gtcgagcgtc gggaaggcgg ccaggtacga ggcgttctcg
     2041 agcgcgccga gcgagcaggc cgcaacgacg ccgaggccga ccgtacccgg ttcgagcccg
     2101 gcccggcgta cgtatcgcgc tatcagcaag gccaggccga gcctgagcgc ctcctcgacg
     2161 ccggcgttgg cggctagcgc ggcccattcc ggcacgcgcc ctaggacggg gtgcagcgct
     2221 ccttggcaca ggcgcatagc gtaccacagc gccgcgccgg cggcgatcgc cgcgagcgtc
     2281 cggagcgtcg tggccgaggc ggccaggatc
//
LOCUS       DAOOLN010000200         2310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_57813_length_2310_cov_2.601774, whole genome shotgun sequence.
ACCESSION   DAOOLN010000200 DAOOLN010000000
VERSION     DAOOLN010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57813_length_2310_cov_2.601774"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /gene="mglC"
                     /locus_tag="PLW80_03405"
     CDS             complement(<1..1041)
                     /gene="mglC"
                     /locus_tag="PLW80_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HPB65580.1"
                     /translation="MMATQEPTALGERLNRRAVNDFMLKYAIYFVFVILIIAITVKEP
                     SFMSIENFRNILSMSATRIIIAMGVGGILITGGTDLSAGRQVGLAAVVSSSMLQALDY
                     PRRMYPELGQLPLIVPILAAMLICGAIGLLNGLVVSKLRVPPFIATLGSMVAVYGATS
                     LYFDRPPYGAQPIGGLDRRFTNLGSGYIGFDGTYSIPFIVLIALGIALIVWVLHNKTT
                     FGKNIYAIGGNPEAAKVSGVNVTKNLLIAYTIAGICYGLAGSLEAARTGGATNNYGNG
                     YELDAIAACVVGGVSTSGGIGTVPGMLVGVLIFSVINYGLTFVNMSPYWQQIVKGAII
                     IAAVALDIRKYLN"
     gene            complement(1050..>2310)
                     /locus_tag="PLW80_03410"
     CDS             complement(1050..>2310)
                     /locus_tag="PLW80_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB65581.1"
                     /translation="TTPGRNSPYRDRSVEENLWLGRYPLKGIGPFKLVDHRKMRADTK
                     ALFKNLNMEIDPAVWVSRLSVSKVQSVEIAKAVSYNAKVIIMDEPTSSLTEHEVAHLF
                     GIIRRLRSEGAAIVYISHKMEEILEIADEVSIMRDGKHVGTWPSGELTTDVIIKRMVG
                     RDLTHRFPPRSNVPGEVVLKVENFTSAERNSFKDVSFELRQGEILGIGGLVGAKRTEL
                     VESIFGLRFVESGVITLRGKEIRIKSPVQAMQDGFSLLTEERRATGIVPMLGVKENML
                     LANLRRYVGRTGLINEKRGVDDVRKKIADLRIKTPSHRTLIKDLSGGNQQKVLFAKWL
                     LTEPEILILDEPTRGIDVGAKYEIYTIIAELAASGKSIIMISSEMPELIGMSDRIMVM
                     CAGRKTGELDGRTATQEEIMRLAALFE"
BASE COUNT          399 a          694 c          745 g          472 t
ORIGIN      
        1 gttcaggtac ttgcggatat cgagggccac cgccgcgata atgatcgctc ccttgactat
       61 ctgctgccag tacggggaca tgttcacgaa ggtcaggccg tagttgatca cgctgaagat
      121 cagcacgccg acgagcatgc ccggtacggt gccgatgcct ccggaggtcg atacgccgcc
      181 gaccacgcag gccgcgatgg cgtcgagctc atagccgttg ccgtagttgt tcgtcgcgcc
      241 gccggtgcgc gcggcctcca gggaacctgc caggccgtag cagatgccgg cgatcgtgta
      301 ggctatgagc aggttcttcg tcacgttgac gccggacacc ttggccgcct ctggattgcc
      361 gccgatggcg tagatgttct tgccgaaggt cgtcttgtta tgcagtaccc agacgatcag
      421 ggcgatgccg agggctataa gcacgatgaa cggtatggag taggtgccgt cgaacccgat
      481 atagccggag ccgaggttcg tgaagcggcg gtcgaggccg ccgatcggct gagctccgta
      541 gggaggccga tcgaagtaca gcgaggtggc gccgtagacg gcgaccatcg agcctagggt
      601 ggcgatgaac ggcggtacgc gcagcttcga cacgacgagg ccgttgagca ggccgatcgc
      661 gccgcagatg agcatcgccg ccaggatcgg tacgatcagg ggcagctggc cgagctcggg
      721 gtacatgcga cgcgggtaat cgagggcctg caacatggag ctcgatacga ccgccgccag
      781 gccgacctga cggccggcgg acaggtccgt gccgccggtg atcaggatgc cgccgacgcc
      841 catcgctatg atgatgcggg tggccgacat cgagaggatg ttcctgaaat tctcgatcga
      901 catgaacgac ggttccttga ccgtgatggc gatgatcagg atcacgaaga cgaagtagat
      961 cgcgtacttc agcatgaagt cgttgacggc ccgcctgttc aagcgttcgc cgagcgccgt
     1021 gggttcttgt gttgccatca tgttctccct tattcgaaca gcgccgccag gcgcatgatt
     1081 tcttcttgag tcgcggtcct gccatcgagc tcgccggtct tcctaccggc gcacatgacc
     1141 atgatcctgt ccgacatgcc gatcagctcc ggcatctcgg agctgatcat gatgatgctc
     1201 ttgccgctgg cagccagctc ggcgatgatc gtgtagattt cgtacttggc gcctacgtcg
     1261 atgcctcggg tcggttcgtc gagtatcagg atctcgggct ccgtcaggag ccacttggcg
     1321 aataggacct tctgctggtt gccgccggac aggtccttga tcagggtccg gtggctcggg
     1381 gtcttgatgc gcagatcggc tatcttcttc ctcacgtcgt cgacgccgcg cttctcgttg
     1441 atcaagccgg tcctgccgac gtagcggcgc aggttggcga gcaacatgtt ctccttgacg
     1501 ccgagcatcg gtacgatgcc ggtggcgcgc ctctcctcgg tcaggagcga gaacccgtcc
     1561 tgcatggcct ggaccggcga tttgatccgt atctctttgc cgcggagcgt gatgacgccg
     1621 gattcgacga agcgcaggcc gaagatcgac tcgacgagct cggtgcgctt ggctccgacc
     1681 aggccgccta tgccgaggat ctctccttga cgcagctcga agctcacgtc cttgaacgag
     1741 tttcgctccg cggaggtgaa gttctcgacc ttgagcacga cctcgcccgg cacgttcgac
     1801 cgcggcggga agcggtgcgt caggtcgcgg cccaccatgc gcttgatgat aacatcggtg
     1861 gtcagctcgc cggacggcca ggtgccgacg tgcttgccat cgcgcatgat cgacacctcg
     1921 tcggctatct cgaggatctc ttccatcttg tgcgatatat agactatcgc ggccccttcg
     1981 gagcggagcc ggcgtattat cccgaacagg tgggcgacct cgtgctccgt cagggagctc
     2041 gtcggctcgt ccatgatgat gaccttggcg ttgtaggata ccgccttggc aatctccacc
     2101 gactggacct tggatacgga cagtcggctg acccagaccg ctgggtcgat ctccatattg
     2161 aggttcttga acagcgcctt ggtgtcggcg cgcatcttcc tgtgatcgac cagcttgaag
     2221 gggccgatgc ccttcagggg atagcggccg agccagagat tctcctccac gctgcggtcg
     2281 cgatagggac tgttgcggcc cggcgtcgtc
//
LOCUS       DAOOLN010000201         2305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_57997_length_2305_cov_2.847111, whole genome shotgun sequence.
ACCESSION   DAOOLN010000201 DAOOLN010000000
VERSION     DAOOLN010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57997_length_2305_cov_2.847111"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1681
                     /locus_tag="PLW80_03415"
     CDS             <1..1681
                     /locus_tag="PLW80_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB65582.1"
                     /translation="AEARSLIESARSAMAESADRARELKERYEAYRLTTPSFIDEFLS
                     PEGIITKKRSVDGRMAAIKDGVTERRERIATAREENRELASRIDEYRKTLEELRGNRI
                     RMQTQAQSAEEQLAMIRREIASQEGYYRELDAEAAMEGRRLSEAEEDLSSIDEEIADI
                     EKRGRELTAELERLEKDIAIKNSDLGKRQEELKKKIDKLGSLQTELERLHLGLAQTET
                     EIRNVQDNFREQYGRELVEFEERMYELRSQAAELRDMLSDRKQRLKDLGSVNLMAPEE
                     YTEVKERYEFLSTQLADLQKARDDLRRITQEIRDESAEMFLNTYNRVKKNFHNMFRRL
                     FGGGRGELRLSDPDHVLESGIEIFAQPPGKKLENITLLSGGEKSLTAIALLFATYMVR
                     PSPFCFLDEIDAALDEQNVIRFVNLLREFGRTSQFIVITHNKKTVTCADALLGVTMEE
                     SGVTKVIAVRLERSDGQPITMPAPSEPFIDEDVEYEAGRELTLEPEAAKPEKARAKAD
                     EATARASDHEETDVVSEAVAIVATTDDTVADVEAIRDDGLQASGDAGTTEP"
     gene            1678..2181
                     /locus_tag="PLW80_03420"
     CDS             1678..2181
                     /locus_tag="PLW80_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65583.1"
                     /translation="MRFPPALAARLGAVAKPALGLARAARAAVGKALAALAEKLRGLS
                     PRGKAIARLSVVGCLAFATAIALIAMTAGSNARRDGARLAPSAEPSVASVRSEPTVPG
                     SGPALASMLLIPGDGERPWPLALEPKPRYTDADAAELSPDLGAVEIGDLSRRRKVALE
                     TIYGAVH"
BASE COUNT          450 a          751 c          777 g          327 t
ORIGIN      
        1 ggccgaggcg cgttccctga tcgagagcgc caggagcgcg atggccgagt cggcagaccg
       61 ggcccgcgag ctcaaggaac gctacgaggc gtaccgcctg acgacgccgt cgttcatcga
      121 cgagttcctg tcccccgagg gcatcatcac caagaagcgt tccgtcgatg ggcgcatggc
      181 ggcgatcaag gacggcgtga cggagcggcg cgagcgcatt gccacggccc gcgaggagaa
      241 ccgcgagctg gcttcccgta tcgacgagta ccgtaagact ctcgaggagt tgcgcggcaa
      301 tcgtatcagg atgcagaccc aggcccagtc ggccgaagag cagctcgcca tgatcaggcg
      361 ggagatagcc agccaagagg gctactatcg cgagctcgac gccgaggccg cgatggaggg
      421 ccgcaggctg tcggaggcgg aggaggatct ctcgtcgatc gacgaggaga tcgccgatat
      481 cgagaaacgt ggccgcgaac tgaccgccga actcgagcgc ctcgagaagg atatagccat
      541 caagaacagc gacctcggca agcggcagga ggagctgaag aagaagatag acaagctcgg
      601 ttcgctgcag acggagctcg agcggctgca cctcggcctg gcccagaccg agaccgagat
      661 acgcaacgtc caggacaact tccgcgagca gtacggccgc gagctcgtcg agttcgagga
      721 gcggatgtac gagctgcgct cccaggccgc cgagctccgc gacatgctgt ccgaccggaa
      781 gcagcggctc aaggacctcg gctcggtcaa cttgatggcg ccggaggagt ataccgaggt
      841 caaggaacgc tacgagttcc tgtcgaccca gctcgccgac ctccagaagg cccgcgacga
      901 cctcaggcgc atcacccagg agatacgcga cgagagcgcg gaaatgttcc tgaataccta
      961 caaccgcgtc aagaagaact tccataacat gttcaggcgc ctctttggcg gcggccgagg
     1021 cgagctacgc ctgagcgacc cggaccacgt actcgagtct ggcatcgaga ttttcgcaca
     1081 gccgcccggg aagaagctcg agaacatcac gctgctgtcg ggcggcgaga agagcctgac
     1141 cgccatcgcg ctgctgttcg ccacctacat ggtcaggccg tcgccgttct gcttcctgga
     1201 cgaaatcgac gcggcgctcg acgagcagaa tgtcatccgc ttcgtcaacc tgctgcggga
     1261 attcggccgt acgagccagt tcatcgtcat cacccacaac aagaagacgg tcacctgcgc
     1321 cgacgccctg ctcggcgtga cgatggagga gtccggcgtg accaaggtga tagccgtcag
     1381 gctggagcgc tccgacggcc agcccatcac gatgcccgct ccgtccgaac ccttcatcga
     1441 cgaggacgtc gagtacgaag ctggtcgcga actgaccctg gaacctgagg ccgccaaacc
     1501 tgagaaggcc agggccaagg ccgacgaggc gaccgcgcga gcttccgacc acgaggagac
     1561 cgatgtcgta tccgaggccg tcgctatcgt cgccacgaca gacgacacgg tggccgacgt
     1621 cgaagcgatc cgcgatgacg gccttcaggc ctcaggcgac gcggggacga cggaaccatg
     1681 aggtttccgc cggccctggc ggctcggtta ggtgccgtag ccaagccggc gctaggcctc
     1741 gcgcgagcgg cgcgcgcggc cgtcggcaag gcgctggcgg cgttggccga gaagctgcgc
     1801 ggcctgtcgc ctcggggtaa ggccatcgcg cgcctctccg tcgtcggctg cctggcgttc
     1861 gctacggcca tcgcgctcat agcgatgacg gccgggtcga acgctcgccg agacggcgct
     1921 cgactcgcgc cgtccgcgga accgagtgtc gcttcggtac ggtcggagcc gaccgtgccg
     1981 ggctccggcc cggccctcgc gtcgatgctc ctgattccag gcgacggcga acgcccctgg
     2041 ccgctggcgc tcgagccgaa gccgaggtat actgacgcgg acgcggcgga actgagcccg
     2101 gacctaggcg ccgtcgaaat cggcgacttg agccgtcgca ggaaggtagc acttgaaact
     2161 atctacggcg cggtccacta gcggctacat gatagctgcg gccttagcga ccctgagcgc
     2221 gttctcgtgc gcgggcggcc cggaaccgct ggcggcggac gaggccgtcg tggcggctga
     2281 accgatcgct gcctcggacg ttcca
//
LOCUS       DAOOLN010000202         2298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_58259_length_2298_cov_3.405707, whole genome shotgun sequence.
ACCESSION   DAOOLN010000202 DAOOLN010000000
VERSION     DAOOLN010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58259_length_2298_cov_3.405707"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLW80_03425"
     CDS             complement(<1..251)
                     /locus_tag="PLW80_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPB65584.1"
                     /translation="MGEKRRTYPEELKRNALELLKTSEKSAAAVARDLGIPAGLLSRW
                     RREAAQEGNGIKAFTGQGVPRDEEMARKSLYAVPFLSSLT"
     gene            complement(1225..>2298)
                     /gene="pepT"
                     /locus_tag="PLW80_03430"
     CDS             complement(1225..>2298)
                     /gene="pepT"
                     /locus_tag="PLW80_03430"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: protein motif:HMM:NF003976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HPB65585.1"
                     /translation="SAPDTSLEVSRWAITPGYEKKPTIGLMAHLDTSSEVSGADVKPR
                     VVEGYDGKALTLSPGWVLDPDEYAELRDYAGDTIVVTDGTTLLGADDKGGIAVVMTAI
                     AAVLKDPSFAHGPIVLVFSPDEETGKGMDGFPAQAVRMDACYTFDGGKGGEIESECFT
                     AYEAKLAFAGKSSHPGYARGVMANAVSMAASFVAMLPRSESPEATDGWYGYYYAHEIH
                     GGLESASVDVLLRDFSDEGMARRQATVRALAAAVEAQFPGGKVTVDLRKQYLNMKKKL
                     DASPKVLELLYEAARKAGAEPYSKPIRGGTDGARLTEMGIPTPNVFAGMHNFHGRHEW
                     ASASEMVLAVDTTLELIRLWAKA"
BASE COUNT          381 a          704 c          739 g          474 t
ORIGIN      
        1 gtgaggctgc tcaggaaggg aacggcataa aggctttttc gggccatctc ctcgtcccgt
       61 ggcacgcctt ggcccgtgaa agcctttatg ccgttccctt cctgagcagc ctcacgcctc
      121 cagcgactga ggagtccggc agggattccc aagtccctgg ccaccgcggc cgcactcttt
      181 tctgaagtct ttaggagctc caaggcattg cgctttagct cctccgggta ggttcttcgt
      241 ttctctccca ttttctctcc ctcctgtagt ctgggagaag cttaactaat tgtccactaa
      301 aacgggggag ttccattgcg gcgcctcata gcggcgcgca gtccccgacg ctcggctcga
      361 gtatcaggcg ccgtcgttga cctgcgagtg aaagcgagtc aggtcgaacg atggccggcg
      421 atcgcggcct attggcccgc gattgcgctt gttgacctgc gagcatcgta gtctattgtc
      481 gaacgatggc cggcgatcgc cgcgacagtc ggcgatcgcg ataatgcgag gcctgggtat
      541 gagcaaggat ctcggggcgg cgcgtcgcga ccggttagcc gggtgggtgt cagtcgcctg
      601 aattacgttc ggcggggcgg gtttcagact ccggtatatt ctatcgaagc gatcatggcg
      661 cccgattgcc ttgtactatc gtgtcgatcc cgtcgccatt attctaatca ccccggcctc
      721 cgcggtaatg cgaggcctgg ttcgtgctac atatagactg gtccgccatg cacaaccgct
      781 agtccgccat gcacaaccgc tagtccgcca tgcactattg atagaacatg atgcattaga
      841 actagaatct gctgtattgt cgcaggacgt tggtgcagtg tgttaaatat aactcgcaca
      901 agcataaatc cgccatgcac aagcgtacat ccgccatgca ttgtcgatag tacctggtgc
      961 atagtcgctg gaaggcggcg taccatgatc ggtcagcgac ttggagagct tccctgacgg
     1021 cggaggtcga ttctcgagcc gctgaacaac gtaccggagc gacttggaat ggttcaggcg
     1081 aaggcggatc ctgacgatca ggatggaggc cggatgaaca cgatgcaggc tatgtactcc
     1141 tcatcgtgtc catcttcttc catcccgaca tcgtgttccc gtcttctcgt atgcagcttc
     1201 ctccgcgcct tggcggcggc tcggtcaggc cttcgcccag agccggatca gctcgagggt
     1261 ggtgtcgacg gcgaggacca tctcggaggc gctggcccac tcgtggcggc cgtggaagtt
     1321 gtgcatgccg gcgaagacgt tcggcgtcgg tatgcccatc tcggtcaggc gagcgccgtc
     1381 ggtgccgccg cgtatcggct tggagtacgg ctccgcgccg gccttgcggg cggcctcgta
     1441 caggagctcg agcaccttgg ggctcgcgtc gagcttcttc ttcatgttca ggtactgttt
     1501 gcggaggtcg acggtgacct tgccgccggg gaactgggcc tcgacggcgg cggcgagcgc
     1561 cctgacggtc gcctggcgcc tggccatgcc ctcgtcggag aaatcgcgga gcagcacgtc
     1621 gacgctggcc gattcgaggc cgccatggat ctcatgggcg tagtagtagc cgtaccagcc
     1681 atcagtcgcc tcggggctct cgcttcgcgg cagcatcgcg acgaagctgg ccgccatcga
     1741 cacggcgttg gccatcacgc cgcgggcgta gccgggatgg gacgacttgc cggcgaaggc
     1801 cagcttggcc tcataggcgg tgaagcactc ggattcgatc tcgcctccct tgccgccgtc
     1861 gaaggtatag caggcgtcca tccgcaccgc ctgggcgggg aagccgtcca tgcccttgcc
     1921 ggtctcctcg tcgggggaga agacgaggac gatggggccg tgcgcgaagg aagggtcctt
     1981 gaggacggcg gcgatcgcgg tcatcacgac ggcgatgccg cccttgtcgt cggccccgag
     2041 cagggtggtg ccgtcggtca cgacgatggt gtcgccggcg tagtccctga gctcggcgta
     2101 ctcatctggg tccaggaccc agcccggcga cagagtcagg gccttgccgt cgtagccctc
     2161 gacgactcgc ggcttaacgt cggcgccgga tacctcgctg gaggtgtcca ggtgggccat
     2221 caggcctatc gtcggcttct tctcgtagcc gggcgtgatg gcccacctgg acacctccag
     2281 cgaggtatcc ggcgccga
//
LOCUS       DAOOLN010000203         2298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_58269_length_2298_cov_2.578243, whole genome shotgun sequence.
ACCESSION   DAOOLN010000203 DAOOLN010000000
VERSION     DAOOLN010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58269_length_2298_cov_2.578243"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLW80_03435"
     CDS             <1..226
                     /locus_tag="PLW80_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HPB65586.1"
                     /translation="NAREALGEANRRLGEVRGALLFNCVLRYLELKASRSTDAFNDIF
                     AAAPFIGFNTFGEELFTHHNQTLTAIFIGR"
     gene            231..1037
                     /locus_tag="PLW80_03440"
     CDS             231..1037
                     /locus_tag="PLW80_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPB65587.1"
                     /translation="MDATEPAIAGSERRTLHYLNSKFKASLFEVVSRSELLSATVAGL
                     NRRFLDMTGLFKRVGVSLKASMSAFSATFDEAGARVEEADRSFSSIESVFDEAYSLSE
                     ALRKEAANAGEQLAVIDDIAETMSILSLNASIQAARAGGAGKAFAVVAAEIRRHTVST
                     KESIERTGRSVDFLVRTIASLAADMDRIRDEVRSGARVMKELVEIIERERVELSSVNA
                     DLEAVDRGFEDYEAIKAALERMIGQSAVSKTEIERILLSYQRHLDAIERT"
     gene            1104..>2298
                     /locus_tag="PLW80_03445"
     CDS             1104..>2298
                     /locus_tag="PLW80_03445"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB65588.1"
                     /translation="MIESDVLVIGAGLSGLTCAALLAERGLSVRVLEACATPGGSCSS
                     FRRGAVTYDLGAALLFGFDGGFSPHRYLMDELGEPVDVYRHEAPYSVRYGERRIVFHR
                     DVNRFIDELAEAFPGTRDSLRAFYDELYALHPLIAGSSASYVPPSEMRLKDLAPAKGA
                     DPRDSLKALTLLAKSAESLARKHLRDVSLIRFFDKLTSTYCYTTMPETPAVLAVTMFV
                     ENHEGGAYSIAGGPAMLAGRLEKAVEDRGGSIEYGAPASRILFDGRRAVGAATEDGRE
                     FRARDVVFTGALSQLASGLVPERLLPRRWKKRVLGYEMTYRNFMVYGTVDRAALPPDA
                     MPIEVFIDNKEAIDEGDVTLYLESLEDPRLAPEGRCSFMLLGPTFSDWPAPGTAAHAD
                     PAYEAA"
BASE COUNT          386 a          764 c          784 g          364 t
ORIGIN      
        1 gaacgccagg gaagccctcg gggaagcgaa ccggcggctc ggcgaggtac gcggagcgct
       61 cctgttcaac tgcgtgctgc ggtacctgga actcaaggcc tcgaggtcga ccgacgcctt
      121 caacgatata ttcgcggccg cgccgttcat cgggttcaat accttcggcg aggagctatt
      181 cacgcaccac aaccagacgc tgacggcgat cttcatcggg aggtagggag atggacgcga
      241 cggaacccgc tatcgccgga tcggagcgga ggacgctcca ttacctgaac agtaagttca
      301 aggcatcgct gttcgaggtc gtatcgcgca gcgagctgct atcggcgacg gtcgcggggc
      361 tcaaccggcg cttcctcgat atgacgggac tgttcaagcg agtcggggtc tcgctcaagg
      421 ccagcatgag cgcgttctcg gcgaccttcg acgaggccgg agcgcgcgtc gaggaggccg
      481 accggagctt ctcgtcgatc gagtccgtct tcgacgaggc gtactcgctg agcgaggcgc
      541 tcaggaagga agcggcgaac gcgggggagc agctggcggt catcgacgac atcgccgaga
      601 ccatgagcat cctttcgctc aacgcgtcca tccaggcggc gagggccggc ggcgccggca
      661 aggccttcgc cgtcgtggcg gcggagatca ggcggcacac ggtctcgacc aaggagtcca
      721 tcgagcggac gggcaggagc gtcgatttcc ttgtgaggac gatagcgtcg ctggccgcgg
      781 acatggacag gatcagggac gaggtgagat ccggcgcccg cgtcatgaag gagctcgtcg
      841 agatcatcga gcgcgagcgc gtggagcttt cgtcggtcaa cgcggacctc gaggccgtcg
      901 accggggctt cgaggactac gaggccatca aggcggccct ggagcgcatg atcgggcagt
      961 cggcggtatc gaagaccgag atagagcgca tcctgctctc gtaccagcgc catctcgacg
     1021 cgatcgagcg gacatagcgc cgcggggccg cgccgcgttt ttttcttgcg gcctcgcgcc
     1081 gccgcgtata tagtcgtatt cccatgatag aaagcgatgt actagtcatc ggcgccggcc
     1141 tgtccggcct gacctgcgcc gcgctgctcg ccgagcgcgg actatcggta cgggtgctcg
     1201 aggcctgcgc gacgcccggc ggctcctgtt cgtccttcag gcgcggagcg gtcacctacg
     1261 acctcggcgc ggccctgctt ttcggcttcg acggcggctt ctctccccac cgctacctga
     1321 tggacgagct cggcgagccc gtagacgtct accgccacga ggcgccgtac agcgtgcgct
     1381 acggcgagcg ccgcatcgtg ttccatcgcg acgtgaaccg cttcatcgac gagctggcgg
     1441 aggccttccc cggcacccgc gactcgctgc gcgcgttcta cgacgagcta tacgccctcc
     1501 acccgctcat agccggctcg tcggcctcgt acgtgccgcc gagcgagatg cggctcaagg
     1561 acctggcgcc cgccaaggga gcggacccgc gcgactcgct caaggcgctg accctattgg
     1621 ccaagagcgc cgagtcgctc gcccgcaagc acctgcgcga cgtgtcgctg atacggttct
     1681 tcgacaagct gacctcgacc tactgctaca ccacgatgcc ggagacgccc gcggtgctcg
     1741 cggtcacgat gttcgtcgag aaccacgagg gcggggcgta ctcgatagcc gggggcccgg
     1801 cgatgctcgc cggccggctc gaaaaggccg tggaggatcg cggcggctcg atcgagtacg
     1861 gcgcccccgc gagccgtatc ctgttcgacg gccggcgcgc ggtcggggcc gcgaccgagg
     1921 acggccgcga gttccgcgcg cgcgacgtgg tcttcaccgg cgcgctgtcg cagctggctt
     1981 cagggctcgt gcccgagcgg ctactgccga ggcgctggaa gaagcgcgtc ctgggctacg
     2041 agatgaccta ccgcaacttc atggtctacg gcaccgtcga cagggcggcc ctgccccccg
     2101 acgcgatgcc catcgaggtg ttcatcgaca acaaggaagc gatcgacgag ggcgacgtca
     2161 cgctctacct ggaatctctc gaggacccga gactggctcc ggaggggcga tgctccttca
     2221 tgctgctcgg gccgacattc tccgactggc cggcccctgg gacggcggcc catgccgacc
     2281 cagcctacga ggcggcca
//
LOCUS       DAOOLN010000204         2296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_58337_length_2296_cov_3.262383, whole genome shotgun sequence.
ACCESSION   DAOOLN010000204 DAOOLN010000000
VERSION     DAOOLN010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58337_length_2296_cov_3.262383"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..1128
                     /locus_tag="PLW80_03450"
     CDS             160..1128
                     /locus_tag="PLW80_03450"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPB65589.1"
                     /translation="MSAGVAQRATFGRALVELAERYPKMVVLDPDVCPSTQTSLFRAA
                     YPDRFYPMGIAEANAVCVAAGLASCGFVPWVSAFAVFLATRAADQVRVSVAHARQPVK
                     LNGAYGGLPSGRGGATHSAVEDIAVMRTMPNMIVLTPADAAEAGAMVELAMETSAPVY
                     LRTMRCELPDLFGVGWRPDIGRAVELAPGDDVAILSEGMMSARALEAAAILRRDGIAA
                     RVLHFGTIKPFDAEAVAKAARECGALVSVENHSRIGGLGSAVCEAAAESDPAVVVRLG
                     FPDCFLESGDDERIFSRYGLDPAGIAAGAREALRRNGSDVSIRRAT"
     gene            1125..1757
                     /locus_tag="PLW80_03455"
     CDS             1125..1757
                     /locus_tag="PLW80_03455"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF006753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HPB65590.1"
                     /translation="MRLAILSETSAADRNADILRALEPRGYELLNCGMRRSGEAPELS
                     YIHTGLMAALLLGLGRVDFVIGGCGTGQGFLNAALLYPGVSCGHVLCPLDAWLFARIN
                     GGNCVSLALNQGYGWAGDVNLRLVFEQLFTPERGAGYPDHRSIPQRASRELLGRLSNL
                     THRPMAELVSALPDEVALPALRYPGFLEAALTGRPKDEALAAAMAARAEA"
     gene            1754..>2296
                     /locus_tag="PLW80_03460"
     CDS             1754..>2296
                     /locus_tag="PLW80_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HPB65591.1"
                     /translation="MSPAVKSLVSRSMPGYTGAAARAAAIARAGGLDEALASGAVQRD
                     QDVPLAEALVLGLLRQGVHKYVGVFGHGSTAVGEALRIYEAAGLVRAFNVRSEIEASH
                     VAAALRWQYGETAAVFTSIGPGALQALAASIAPASNGLGLYYLFGDETSQDEGYNMQQ
                     IPRQEQVCFLKAASAMGAAYS"
BASE COUNT          315 a          781 c          821 g          379 t
ORIGIN      
        1 tatcgcgtcg gcgcgcgagc gagccgggcg cggtcccgtc gccctggtcg ctacgaccgt
       61 aaagggcaag ggcgtctcgt tcatggaagg acgctgcgaa tggcacggca aggcgcccga
      121 cgacggcgag ttggcccgcg cgctggcgga gctcgggtca tgagcgccgg cgtcgcgcaa
      181 cgggccacct tcggacgggc cctcgtcgag ctggcggagc ggtatccgaa aatggtcgtc
      241 ctcgacccgg acgtctgtcc ctcgacgcag acctcgctgt tccgcgccgc ctatcccgac
      301 cgcttctatc cgatgggcat agccgaggcc aacgccgtct gcgtcgccgc cggcctggcg
      361 agctgcggct tcgtgccctg ggtctccgcc ttcgccgtct tcctggcgac cagggccgcg
      421 gaccaggtcc gcgtctcggt cgcccatgcc cgccagccgg tcaagctcaa cggggcgtat
      481 ggcggccttc cttcgggccg gggcggggcg actcactcgg ccgtcgagga tatagccgtg
      541 atgcggacga tgccgaacat gatcgtgctg acgcccgccg acgcggccga ggccggagcg
      601 atggtcgagc tggcgatgga aacctccgcc ccggtctact tgcggacgat gcgttgcgag
      661 ctgcccgacc tgttcggcgt cggttggcgc cccgatatag gtcgcgcggt ggagctggcg
      721 ccgggcgacg atgtggctat cctatcggag gggatgatga gcgcgcgcgc cctcgaggcc
      781 gcggccatcc tgcgccgtga cggcatcgcc gcgagggtgc tccatttcgg taccatcaag
      841 ccgttcgacg ccgaggccgt ggcgaaggcc gcccgcgagt gcggggcgct cgtcagcgtc
      901 gagaaccatt cgcggatcgg cggcctcggc tccgccgtct gcgaggcggc ggcggagtcg
      961 gaccccgccg tcgtcgtccg gctgggcttc ccggactgtt tcttggaatc gggcgatgac
     1021 gagcgcattt tctctcggta cgggctcgac ccggccggta ttgcggccgg cgccagggaa
     1081 gcgctacgcc ggaacggttc cgacgtctct attaggaggg cgacatgagg ctagccatac
     1141 tgagcgagac gagcgcggcc gaccggaacg ctgatattct tcgtgccctc gagccgcgcg
     1201 ggtatgagct cctgaactgc ggcatgcgcc gctcgggcga ggcgcccgag ctatcctaca
     1261 tccatacggg cctgatggcg gccctgctcc tgggccttgg ccgagtcgat ttcgtcatcg
     1321 gcggctgcgg cacgggccag ggcttcctga acgcggccct cctctacccg ggcgtgtcct
     1381 gcggccacgt cctctgtccc cttgacgcgt ggctgttcgc ccgcatcaac ggcggtaact
     1441 gcgtaagcct ggccttgaac cagggctacg gctgggccgg cgacgtcaat ctccgcctcg
     1501 tgttcgagca gctgttcacg ccggagcgcg gcgcgggcta tccggatcac cgtagcatac
     1561 cccagcgcgc ctcgcgcgag ctgcttgggc ggctgtcgaa cctgactcat cgcccgatgg
     1621 ccgagctcgt gtcggccctg cccgacgagg tggccctccc cgctctacgc tatccgggct
     1681 tcttggaagc ggcgctgacc gggcggccga aggatgaggc cctggccgcc gcgatggcgg
     1741 cgagggccga ggcgtgagtc ccgctgtgaa gagcctcgtg agccgctcta tgccaggcta
     1801 caccggcgcg gccgcaaggg cagccgccat cgcgagggcc ggcgggcttg acgaggcgct
     1861 cgcctcgggg gcggtacaac gcgaccaaga cgtgccgctg gccgaggccc tcgtgctggg
     1921 gctgttgcgc cagggcgtac ataagtacgt cggcgtcttc ggacacggct cgacggccgt
     1981 aggcgaggct ctcaggatct acgaggctgc cggcctcgtg cgggcgttca acgtccgtag
     2041 cgagatcgag gcgtcgcacg tggccgccgc gctcaggtgg cagtacggcg agaccgccgc
     2101 ggtctttact tcgataggtc cgggcgccct gcaggctttg gcggcgtcga tagccccggc
     2161 ctcgaacggg ctcgggctct actacctgtt cggggacgag acgagccagg acgagggcta
     2221 taacatgcag cagatcccgc gccaggaaca ggtttgcttc ctcaaggccg cctcggctat
     2281 gggcgccgcg tacagc
//
LOCUS       DAOOLN010000205         2295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_58393_length_2295_cov_2.025893, whole genome shotgun sequence.
ACCESSION   DAOOLN010000205 DAOOLN010000000
VERSION     DAOOLN010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58393_length_2295_cov_2.025893"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /gene="trpS"
                     /locus_tag="PLW80_03465"
     CDS             <1..959
                     /gene="trpS"
                     /locus_tag="PLW80_03465"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPB65592.1"
                     /translation="YECFFIIADLHTLTTKPEKEHIEALADNVRSAALDYLACGIDPA
                     RSVIYLQSAVPEVVELSLFFQNLITVPRLSRVPSLKEMAQGAGIEEMPFGLLGYPVLQ
                     AADILLPRAHLVPVGKDNVAHVEVTREIARRFNYLYGDTFPVPDAMVSEYGSLVGTDG
                     NAKMSKSLNNAIFLSDDERTVEKRVMSMYTDPKRVSADVPGTVEGNPVFQYHDAFNPD
                     KAEVEDLKARYRAGTVGDVEVKQKLAKALNAFLDPIRERRAKYESQSGLADEVVYDGT
                     LRMREEARATLSAAKKAMGMAAVWNRVSRKAEDAKKKRAKGE"
     gene            1324..1947
                     /locus_tag="PLW80_03470"
     CDS             1324..1947
                     /locus_tag="PLW80_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65593.1"
                     /translation="MDIERLQRAEASFMERYPGGFEHPEMLERGRRHRIGRLVERART
                     VLAPERFDDSERMAEDIIKTVASSSMVSIFEKPAFRDLVRSLSAKERARLCSHLFQLL
                     HGDAERGFEDLSAFLASGKMAKWTVMTVFPFYTKPTEEPFVKPTTAKEIIAYYGLEGL
                     AYRPRPYWAFYSGYRGQLRAMMGLVSPLVAPDMGSFSAFLMIGAGAW"
     gene            complement(1952..>2295)
                     /locus_tag="PLW80_03475"
     CDS             complement(1952..>2295)
                     /locus_tag="PLW80_03475"
                     /inference="COORDINATES: protein motif:HMM:NF021386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HPB65594.1"
                     /translation="RRQGYGLGRLRVSGGLRALTERIWMMDAGSLAALLDREGRVTRW
                     PKKQDQKRSVLEYLGGKFSADVEYSEREVNAALDAWHLFGDHALLRRELFERGFLDRT
                     PDGRRYWLPRP"
BASE COUNT          455 a          744 c          725 g          371 t
ORIGIN      
        1 agtacgagtg cttcttcatc atcgccgacc tgcataccct gacgaccaag cccgagaagg
       61 agcatatcga ggcgctcgcc gacaacgtac gctcagccgc cctcgactac ctggcctgcg
      121 gcatcgaccc ggcgcgctcg gtcatctacc tccagtcggc ggtgcccgag gtggtcgagc
      181 tgtcgctgtt cttccagaac ctgatcaccg tgccgcgcct gtcccgcgtg ccgagcctca
      241 aggagatggc ccagggcgcc ggcatcgagg agatgccgtt cgggctcctg ggctacccgg
      301 tgctccaggc cgcggacatc ctcctaccgc gcgcccacct cgtgcccgtc ggcaaggata
      361 acgtggccca cgtcgaggtg acccgggaga tagcccgccg cttcaactac ctgtacggcg
      421 ataccttccc ggtgccggac gcgatggtat cggaatacgg ctccctcgtc ggcaccgacg
      481 gcaacgccaa gatgtccaag agcctcaaca acgcgatctt cctgtctgac gacgagcgga
      541 ccgtcgagaa gcgggtcatg tcgatgtaca ccgaccccaa gcgggtcagc gccgacgtgc
      601 caggcaccgt cgagggcaac cccgtgttcc agtaccacga cgccttcaac ccggacaagg
      661 ccgaggtcga ggacctcaag gcccgctacc gagccggcac ggtgggcgac gtcgaggtca
      721 agcagaagct ggccaaggcg ctcaacgcgt tcctcgaccc gatacgcgag cggcgcgcca
      781 agtacgagtc tcagtccggc ctggccgacg aggtggtcta cgacggcacc ttgcgcatgc
      841 gcgaggaggc ccgcgcgacg ctgtccgccg caaaaaaagc catgggcatg gccgccgtct
      901 ggaaccgcgt ctcccgcaag gccgaggacg ctaaaaagaa gcgagccaag ggcgagtaag
      961 gcggcggacc gaacaaggcg gcgtagtaaa caaggcggca acgagaggat acgagacgaa
     1021 gaggcaggaa agaggaacgc tcgccaagag aatagaaagg aatagagcaa agacggaaca
     1081 ggatgatcag gattcggaca ggatgatcaa gatgcagcag gattcccatg atgaattcct
     1141 gagtatgcgg cctagtcctt atccctcatc atggtcatct tgagaaatcc tgcaacatta
     1201 tgttccgcct ttcgtactac gagccatctt ccttagcgtc tccgcatctc cgcgcgaaat
     1261 tctatcagcc gaggtttagc gcggcgttgc cggggaacgg gctgaggcgg tatcgtatcg
     1321 ggcatggata tagaacggtt acaacgagcc gaagcatcct tcatggagcg ctacccgggc
     1381 ggcttcgagc acccggagat gctcgagcgc ggccgccggc accggatagg caggctggtc
     1441 gagcgggcca ggacggtcct ggcaccggaa cgcttcgacg acagcgaacg catggccgag
     1501 gacatcatca agacggtcgc ctcgtcgtcg atggtgtcga tcttcgagaa accggcgttc
     1561 cgcgacctgg tgaggagcct gtcggccaag gaacgggcca ggctgtgctc ccacttgttc
     1621 cagctgctcc acggcgacgc cgagcggggc ttcgaagacc tctcagcgtt cctggcgtcc
     1681 ggcaagatgg ccaagtggac ggtcatgacg gtcttcccat tctacacgaa gcccacagaa
     1741 gagcccttcg tcaagccgac gacggccaag gagatcatcg cgtactacgg gctcgagggc
     1801 ctggcgtatc ggccccggcc gtattgggct ttctactcgg gctaccgcgg ccagctcagg
     1861 gcgatgatgg gtctggtcag cccgctcgtc gccccggaca tgggctcgtt ctcggccttc
     1921 ctgatgatag gcgccggcgc ctggtagcct ttcagggccg cggcagccag tagcggcggc
     1981 cgtccggcgt ccgatcgagg aagccgcgtt cgaagagctc gcggcgcagg agggcgtggt
     2041 cgccgaacag gtgccaggcg tcgagggcgg cgttcacctc gcgctcggag tactcgacgt
     2101 cggccgagaa cttgccgccc aggtactcga ggacgctccg cttttgatct tgcttcttgg
     2161 gccatcgcgt gaccctgccc tcccggtcga gcaacgccgc caggctgccg gcatccatca
     2221 tccatatcct ttctgttaac gcccgaagac cacccgagac acgaaggcgg cccagtccat
     2281 agccctgacg ccggc
//
LOCUS       DAOOLN010000206         2294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_58420_length_2294_cov_2.958910, whole genome shotgun sequence.
ACCESSION   DAOOLN010000206 DAOOLN010000000
VERSION     DAOOLN010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58420_length_2294_cov_2.958910"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PLW80_03480"
     CDS             <1..719
                     /locus_tag="PLW80_03480"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPB65595.1"
                     /translation="NKGRKPESIEERLIAETGRTLLMTSTSSPLPAVDPYPEGRIVTQ
                     AMADAFEGPSIFVEGSAFERSRAEKAAVGIVSELYCPILYYQYVVGYIYLMNDSSKKT
                     CLDYRAVDFAWEFSRILAYSLKSNDYFKLSEDSKPDPYTPQVVDLSAGGCLLSMPKSS
                     FKVKLKSGAVLDILIGKDGGGSSVAMKGRVARRFDDRDNEYYGVAFINAEQDAYSELR
                     RSLYADESNRFACDEASLEL"
     gene            732..977
                     /locus_tag="PLW80_03485"
     CDS             732..977
                     /locus_tag="PLW80_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65596.1"
                     /translation="MDADFSVMGRLRLAMAVASRWETLVTLGAFVAFWLLLRYIADPW
                     RRDDRKPRGVKKAKKPSPTPVEQAIEADESDDDGFPD"
     gene            complement(934..>2294)
                     /locus_tag="PLW80_03490"
     CDS             complement(934..>2294)
                     /locus_tag="PLW80_03490"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HPB65597.1"
                     /translation="SGKSLCFQLPATLCSGPTLVVYPLLGLMADQARRLKSSGMVAAT
                     LRGGMSANERRAALESVRSGTARFIITNPETLRTPSVAKALAEAKPSHFVIDEAHCVA
                     EWGDSFRPAYLSLGEAIRSIKPRVVTAFTATASPPILTRIAEALFGGEPYRLVAGAPD
                     RPNIRYSVKPALSMRRELRAAVATMPKPLIVFARSRHGVQIIAEDLIGSRSGLDCRFY
                     HAGLEKAERAAVEAWFMGSDDGVLVSTCAYGMGMDKPNVRSVVHYGRPASVEAYLQES
                     GRAGRDGEQSEALLIHKLEPGSRTRGDYEPRLVPEPVSAEARSEAMRRYATGRYGCRR
                     SFLLGVLGYPEAAETACSSCDRCDGTAIEDAPGAAELAAIAARHPRRFDATGLAAFAV
                     GAPGTAVAPRHGTMAGWTTEEAGEAIAAALGAGLLRRRSRWPWKGAISPGTRRRPTRR
                     PR"
BASE COUNT          392 a          752 c          744 g          406 t
ORIGIN      
        1 tcaacaaggg gcgcaagccc gagagcatcg aggaacggct gatagccgag acgggccgga
       61 cgctgctcat gaccagcacc tcgagcccgc tcccggccgt cgacccctac cccgagggtc
      121 ggatcgtgac gcaggccatg gccgacgcgt tcgaggggcc gtcgatcttc gtcgagggct
      181 ccgccttcga acggagccgc gccgagaagg ccgccgtcgg catcgtctcg gagctgtact
      241 gccccatact ctattatcag tacgtcgtcg gctacattta cctgatgaat gactcgtcca
      301 agaagacctg cctcgactat cgggccgtcg atttcgcgtg ggagttctcg cgcatcctcg
      361 cctatagcct gaagtccaac gactacttca agctgtccga ggactcgaag ccggatccct
      421 atacgcctca ggtcgtcgac ctatcggccg gaggttgcct tctgtcgatg ccgaagtcga
      481 gcttcaaggt caagctcaag agcggcgcgg tactcgacat cctgatcggc aaggatggcg
      541 gaggctcgag cgtcgccatg aaaggccgag tcgcgagacg attcgacgac cgggataacg
      601 agtattacgg cgtcgccttc atcaacgccg agcaggacgc ctacagcgag ctacgcaggt
      661 cgctctacgc cgatgagtcg aacaggttcg cctgcgacga ggcttctctg gagctgtgat
      721 cggaggttcg gatggatgca gatttctccg tcatgggacg gctacggcta gccatggccg
      781 tggcctcccg atgggagacc ctcgtcacgc ttggcgcctt cgtcgcgttc tggctactcc
      841 tgcgctatat agcagatcct tggaggcggg acgatcgcaa gccccggggc gtaaaaaaag
      901 ccaagaaacc gtctccgact ccggtcgaac aggctatcga ggccgacgag tcggacgacg
      961 acgggttccc ggactgatag cccctttcca tggccagcgg cttcggcggc gtagcagacc
     1021 cgccccgagg gcggccgcga tcgcttctcc ggcctcttcg gtcgtccaac cggccatcgt
     1081 tccgtggcgc ggcgcgaccg cggttcctgg tgcgccgacg gcgaacgcgg ccaggcccgt
     1141 cgcgtcgaag cgccgtgggt gacgcgccgc gatcgcggct agctcggcgg cgccgggagc
     1201 gtcttcgata gccgtcccgt cgcagcggtc gcaggacgaa caggccgtct ctgccgcctc
     1261 gggatagcct aggactccca gcaggaacga cctcctgcag ccgtagcgcc cggttgcgta
     1321 gcgccgcatc gcctcggacc tggcctcggc cgataccggc tccgggacga gcctcggttc
     1381 atagtcgcct cgtgtgcggc tcccgggctc gagcttatgg ataagcagag cctcgctctg
     1441 ctcgccgtct ctgccggctc gccccgattc ctgaagatag gcctctaccg aagccggccg
     1501 cccataatgc acgaccgagc ggacgttcgg cttgtccatg cccataccgt aggcgcacgt
     1561 gcttacgagg acgccgtcgt cggagcccat gaaccatgcc tcgacggccg cgcgctcggc
     1621 cttctcgagc ccggcgtggt agaagcggca gtcgaggcct gagcgcgagc ctatcaagtc
     1681 ctccgctatg atttggacgc catggcggga tcgggcgaac acgatcagcg gtttcggcat
     1741 ggtagccacg gccgcccgta gttcccggcg catcgacagg gccggcttga cgctgtagcg
     1801 tatgttcggc ctgtcaggag cgcccgcgac gagcctgtat ggctcgccgc cgaaaagcgc
     1861 ctccgcgatg cgcgtgagta tgggaggaga ggccgtcgcg gtgaaggccg tgacgacgcg
     1921 cggctttatg gacctgatcg cttctccgag cgatagatag gccggcctga aactgtcgcc
     1981 ccattcggcg acgcaatggg cctcgtctat cacgaagtgc gacggcttgg cttccgctag
     2041 cgccttcgcc acgctgggcg tccgtagcgt ctctggattg gttatgatga aacgggctgt
     2101 gcccgatcgt accgattcga gggcggcgcg cctctcgttc gccgacatgc cacctcgcag
     2161 cgtcgccgcg accatgccgg agctcttgag cctcctggcc tgatcagcca tgaggccgag
     2221 caggggatag acgacgagcg tcggtccgga gcagagggtc gccggtagct gaaagcagag
     2281 gctctttccg gaac
//
LOCUS       DAOOLN010000207         2294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_58424_length_2294_cov_2.656990, whole genome shotgun sequence.
ACCESSION   DAOOLN010000207 DAOOLN010000000
VERSION     DAOOLN010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58424_length_2294_cov_2.656990"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /locus_tag="PLW80_03495"
     CDS             <1..1458
                     /locus_tag="PLW80_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPB65598.1"
                     /translation="PAAFIVGDAFGRAAVSDGATGLSLINKAGQEAWRVELGARPDSP
                     PAFASDGRLYVSAGGELRAFAPNGRRLWSARPGAAPSSPLVVGPGGGPALGLADGRVA
                     LYHPDSGVATELALGSAPVALAAAGRLAAGLADGRIVVLAAEAGALSPAPGSPAAADA
                     PSLGSRPAALAAASDAFYALGADGVLLAVDGAGVELWRRRAVTDGSTAALAAFEGRAI
                     VLTRSAVTSYGPDGSVYRSLTLRNAVAMPAIAPNGSVFSGGADWILYAYRFERPLTAA
                     AGPVIEPLDLSAVDAMAKEEAFWSVAPWSDDAAMERLYDIEKSMESGTIGMDSRRAAL
                     YLSAVALGRMDAPFGSGRAPTGPAPRGPLPRVYACGLLGRMGLPWAVPVLVEAFRGDP
                     EPSVRAAAAYAVATIGLDPEGRALGAFAEAAGRRLDGATAGAVVDAIDGLYRASGALD
                     DRSGMLALLRIAGGDYARDIRSKAEKALSRVSRGR"
     gene            1502..>2294
                     /locus_tag="PLW80_03500"
     CDS             1502..>2294
                     /locus_tag="PLW80_03500"
                     /inference="COORDINATES: protein motif:HMM:NF017106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P83/100 family protein"
                     /protein_id="HPB65599.1"
                     /translation="MIRGKRLALFAAMALALSAVPLPADDALDVDEAEVSSAQGRAIQ
                     FINYEGPHEVIETAESIRGIGRALGRAVAAGATRSGEVGRYSVIHAVDPAVPAGFDAD
                     IILLGEGARVDHVKNVRRIVAGFLEEAYGYAPADADTLAVFVTIYNAVYRGDMGYFGA
                     RYKPVVTRELSPSTAGMSTRWDEWAGRTRIVIPLSGRAGMGVVGSVDTTPITDAPTVA
                     SLKDESPTAGVAERMDVVDIKERAQDEEKAAIEAEKLRLEREAAAI"
BASE COUNT          305 a          813 c          849 g          327 t
ORIGIN      
        1 ccggccgcct tcatcgtcgg cgacgcgttc ggccgggccg ccgtctccga cggcgctacc
       61 gggctgtcgc tgatcaataa ggccggtcag gaggcctggc gggtcgagct aggggccagg
      121 ccggactcgc cgccggcctt cgcctcggac ggccgcctct acgtcagcgc cggcggcgag
      181 ctccgggcct tcgcgccaaa cggccggcgc ctatggagcg ccaggcccgg agccgccccg
      241 agctcgccgc tggtcgtggg gcccggcggc ggccccgcgc tcggcctggc ggacggccgc
      301 gtggcgctct accaccccga ttccggcgtc gcgacggaat tggcgctcgg ctcggccccg
      361 gtagccctgg ccgccgccgg tcgactggcg gcgggcctcg cggacggacg catcgtcgtc
      421 ctggccgccg aagccggcgc cctatcgccg gcgccaggct caccggccgc cgcggacgcc
      481 ccgagcctcg gctcgcgccc cgcggccctg gccgccgcga gcgacgcctt ctacgcgctc
      541 ggcgccgacg gcgtcctctt ggccgtcgac ggggctgggg tcgagctatg gcgtcggcgc
      601 gcggtgacgg acgggagtac ggcggcgctc gccgccttcg agggccgcgc gatcgtgttg
      661 acccggtcgg ccgttacgag ctacggcccc gacggttcgg tctatcgttc ccttacgctc
      721 aggaacgccg tagccatgcc cgcgatagcc ccgaacggct ccgtcttctc cggcggagcg
      781 gattggatac tctacgccta ccgtttcgag cgaccgctca cggccgcggc cggcccggtc
      841 atcgagccgc tcgacctctc ggcggtcgac gcgatggcca aggaggaggc gttctggagc
      901 gtggcgccgt ggagcgacga cgcggcgatg gaacggctgt acgatattga aaaatccatg
      961 gaatcgggta ctataggtat ggattccagg cgagcggcgc tgtatctttc ggcggtggcc
     1021 ctgggccgga tggacgcgcc attcggatcg gggcgcgctc ctaccgggcc cgcgccgcgc
     1081 gggccgctac cgcgggtcta cgcctgcggc ctccttgggc gcatgggcct gccctgggcg
     1141 gtgcccgtgc tcgtcgaggc cttcaggggc gacccggagc cgtcggtcag ggccgcggcg
     1201 gcctacgccg tggcgacgat aggcctcgac ccggagggcc gagccctcgg cgccttcgcg
     1261 gaagcggcgg gccggcggct cgacggcgcg accgccggcg ccgtcgtcga cgcgatcgac
     1321 ggcctgtacc gcgcgtccgg agcgctcgac gatcggtccg ggatgctggc gttattgagg
     1381 atagcgggcg gcgactacgc ccgggatata cgatccaagg ccgagaaggc gctgtcgcgc
     1441 gtctcgcgag gccggtagcc caaggaagcg cggcggccct cgcgccggga ggagagacgg
     1501 gatgatacga ggcaagcgac tggctctgtt cgcggcgatg gccctggcgc tgtcggccgt
     1561 cccgctcccg gccgacgacg cgctggacgt cgacgaggcc gaggtctcga gcgcccaggg
     1621 acgggcgata cagttcatca actacgaggg accccacgag gtgatcgaga cggccgagtc
     1681 gatacgcggt attggccgcg cgctcgggcg ggccgtcgct gccggcgcta ccaggtccgg
     1741 cgaggtcggc cgttactcgg tgatccacgc ggtcgatccc gccgtgcctg ccggcttcga
     1801 cgccgatatc atcctccttg gcgagggcgc ccgcgtcgac cacgtcaaga acgtgcggcg
     1861 catcgtggcc gggttcctcg aggaagccta cggctatgcg ccggccgacg ccgacacgct
     1921 cgccgtgttc gtcaccatct ataacgccgt ctatcgcggc gacatgggtt acttcggcgc
     1981 ccgttacaaa cccgtcgtca cgagggagct gtccccgtcc accgccggca tgtcgacccg
     2041 ctgggacgaa tgggccggcc gtacgaggat agtcataccg ctgtcgggcc gcgccggcat
     2101 gggtgtcgtc ggctcggtcg acaccacccc gatcaccgac gcgccgaccg tagcgagcct
     2161 caaggacgag tcgcctaccg ccggcgtggc ggagcggatg gacgtggtgg acatcaagga
     2221 gcgggcccag gacgaggaga aggccgccat cgaggccgag aagttgcgac tcgagcgcga
     2281 ggcggccgcg atag
//
LOCUS       DAOOLN010000208         2292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_58492_length_2292_cov_2.542244, whole genome shotgun sequence.
ACCESSION   DAOOLN010000208 DAOOLN010000000
VERSION     DAOOLN010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58492_length_2292_cov_2.542244"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PLW80_03505"
     CDS             complement(<1..603)
                     /locus_tag="PLW80_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65600.1"
                     /translation="MAVLVKNIEREFLLSTAMRERSPITMGAGGGEWTVRILAVEKGK
                     LSFGHELPLDLQRKGSAYDFRYNVRGQTIAFKATMLEPGERRFATSMPDKLYKNLSRR
                     FVRLPPPGDLSASFAFAGERYDLDFPSSAGSGPSREPEPSPYFDPSDLRGLMAEFERK
                     ALAVASDRGIVMFKDRKPETVPERLAATTGRCFYLPTALGG"
     gene            complement(665..1462)
                     /locus_tag="PLW80_03510"
     CDS             complement(665..1462)
                     /locus_tag="PLW80_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65601.1"
                     /translation="MQASRSRLYGIPGAAIVLALAISAHACSKSRERPDSAPAAATSP
                     AAATSQAAREAAALEASGPRDKPDDIPVEKPAETPVAMKASPKASTSVKTDAKASKAI
                     VSSNLAGVDRRLIERPFDALAAGDFELGELYGSGASAPANAALSVALLELERSLLEDE
                     PPYGSMGAEASALARARFGDAFPLSFARVRFARPVVGPALVASVPFRAFADDSGARAI
                     IGLAILAADDDGTWVIEHLELDPEALEDGASRSEPWDPYDTSRQAYE"
     gene            1520..>2292
                     /locus_tag="PLW80_03515"
     CDS             1520..>2292
                     /locus_tag="PLW80_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB65602.1"
                     /translation="MDDIWPRLGIAFDEPVRDPLWRDVMMPTEFEPILTSPDFIKLSR
                     ILQLGPTHLVYPGATHTRRAHSIGVFELAKRSLATVLSRSGPVAGLDGAAARGFLVAA
                     LCHDLGHFPYAHSLKELPLRDHESLTADIVRGSLRPAVEAAGADPDLVAAIVDQGLPA
                     PAGSSAVALRLFRSMLSGVLDPDKLDYLNRDAWACGVPYGQQDVDFVLQQLGLDESGR
                     PGVAERGVMAVEAVLFSKYQMYRAVYWHRTVRAATAMIK"
BASE COUNT          343 a          775 c          781 g          393 t
ORIGIN      
        1 gccgccgagc gccgtcggta ggtagaagca acggccggtg gtggccgcca gccgctccgg
       61 gacggtctcc ggcttgcggt ccttgaacat cacgatgccg cggtcgctgg cgacggccag
      121 ggccttgcgc tcgaattcgg ccatcaggcc gcgcaggtcc gacgggtcga agtacggcga
      181 cggctccggc tcgcgggacg ggcccgagcc cgcgctcgag ggaaaatcca ggtcgtagcg
      241 ctctccggcg aacgcgaagg aggcggaaag gtcgcctggc ggaggcaggc gcacgaaacg
      301 cctgctcagg ttcttgtaga gtttatcggg catcgatgta gcgaagcggc gttctcccgg
      361 ctcgagcatc gtcgccttga aggctatcgt ctggcccctg acgttgtagc ggaagtcgta
      421 ggcggagcct ttacgctgca ggtcgagcgg tagctcgtgg ccgaagctca gcttgccctt
      481 ctcgacggcc aggatgcgca cggtccactc gccgccgccg gcgcccatcg tgatgggcga
      541 acgctcgcgc atcgccgtcg acaggagaaa ttctcgctct atgtttttta ctagcaccgc
      601 catcggcatg gtcctccgat agcattgtgc ctcaatcgcg atgattgttc aacgttttcg
      661 tcgattattc gtaggcctgg cggctcgtat cgtaggggtc ccatggctcc gaccgagacg
      721 ctccgtcctc gagcgcctcc gggtcgagct ccaggtgctc gatgacccag gtcccgtcgt
      781 cgtcggcggc caggatggcc aggccgatga tagcccgggc gccgctatcg tcggcgaagg
      841 ctcggaacgg gacgctggcg accagcgccg gtccgacgac gggcctggcg aaacgtaccc
      901 gggcgaacga tagcgggaac gcgtcgccga acctggcgcg ggccagggcc gacgcctcgg
      961 cgcccatcga tccgtacggc ggctcgtcct caaggaggct acgctccagc tcgagtagcg
     1021 ccacgctcag cgcggcgtta gccggggcgc tcgcgcccga gccgtagagc tcgccgagct
     1081 cgaagtcccc ggcagctagc gcgtcgaaag gccgttcgat gaggcgccgg tcgactccgg
     1141 ctaggttcga cgagacgatc gccttggagg ctttcgcgtc cgtcttgacg gaggtcgagg
     1201 ccttgggcga cgccttcatc gccacgggcg tttcagccgg tttctcgacg ggtatatcgt
     1261 cgggcttgtc gcgaggcccg gaggcctcga gcgcagccgc ttcgcgcgcg gcctggctcg
     1321 tcgccgcggc cgggctcgtc gccgcggccg gcgcgctatc cgggcgctcc ctgctcttgg
     1381 agcaggcatg ggcggagatg gcgagcgcta gtacgatagc ggcgccaggt ataccgtata
     1441 gtcgagatcg gctcgcttgc atcgtggtat agtatctcca gaccgagccg gtcgatcaac
     1501 cggttccgac ggaggcatca tggacgacat ctggccgcgc ctcggcatcg cgttcgacga
     1561 acccgtcagg gatcccctct ggcgcgacgt gatgatgccg accgaattcg aacctattct
     1621 gacgagcccg gatttcatca aactgtcgcg catcctccag ctcggcccga cgcacctggt
     1681 ctatcccggc gccacccata ccaggcgggc ccactccata ggcgttttcg agctggccaa
     1741 gcgatcgctc gcaaccgtgc tgtcgcgctc aggccccgta gccgggctcg acggggccgc
     1801 ggctaggggc ttcctcgtcg cggcgctctg ccacgacctc gggcactttc cgtacgccca
     1861 ttccctcaag gagctgccgc tgcgcgacca cgagagcctt acggccgaca tcgtgcgcgg
     1921 tagcctgcga ccggccgtgg aagccgccgg cgccgatccc gacttggtgg cggccatcgt
     1981 cgaccagggc ctgccggctc cggccggctc gagcgctgtc gcgctccggc tgttccgctc
     2041 gatgctatcc ggcgtgctcg acccggacaa gctcgattac ctgaatcgcg acgcctgggc
     2101 ctgcggcgtg ccctacggcc agcaggacgt cgacttcgtg ctgcagcagc tggggctcga
     2161 cgaatccggc cggcccgggg tggccgagcg cggcgtgatg gccgtcgagg cggtgctgtt
     2221 ctccaagtac cagatgtacc gggcggtcta ctggcaccgg acggtccgcg ccgcgaccgc
     2281 gatgatcaag aa
//
LOCUS       DAOOLN010000209         2291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_58523_length_2291_cov_2.910555, whole genome shotgun sequence.
ACCESSION   DAOOLN010000209 DAOOLN010000000
VERSION     DAOOLN010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58523_length_2291_cov_2.910555"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1758)
                     /locus_tag="PLW80_03520"
     CDS             complement(<1..1758)
                     /locus_tag="PLW80_03520"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313049.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HPB65603.1"
                     /translation="MKIDRLFTTAGKGPYEGVRWEKRVSEIRNPDGKAVFRMDGVIVP
                     STWSQIATDIIAQKYFRKAGVPDDKAEAWRAWVPSSQQALAGASPVPGSEHDARQVFH
                     RLAYTWTLWAKESGYFDSKADEKAYYDEMCFMLANQMGAPNSPQWFNTGLHAVYGLEG
                     PAQGHYYVDPKSGQVVASTNAYERPQPHACFILDVQDDLVNDGGIMDLVEREARLFKY
                     GSGTGSNFSKIRGAGEPLSGGGFSSGLLSFLKIADRSAAAIKSGGTTRRAAKMVILDA
                     DHPDIEQFVDWKAGEEYKVACLVAGSKALKQYSSAMRDAAARSDVPASDRLSIDANPW
                     LRSAAKEALDAGVPGSYVYQLLRRIADGDPDVDGPVYDASWEGEAYNTVSGQSSNNSV
                     RVTNEFMEAVAKGKDWALTMRRGGKTLRTIKARSLWDRIAKSAWQCADPGLQFHTTIN
                     DWHTCPADGEIRGSNPCSEYMFLDDTACNLASLKLTAFYDERARSFDVDRYRHAVRLW
                     TLTLEISVAMAQFPSKEIARKSYDYRTLGLGYANLGTLLMLMGLPYDSAEGRAVAAAI
                     SAIMTGEAYAHSARMAAAFG"
     gene            1895..>2291
                     /locus_tag="PLW80_03525"
     CDS             1895..>2291
                     /locus_tag="PLW80_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65604.1"
                     /translation="METLAIINAIGLSEHATSPWADGPSALERAVAFAERLAPGRPGA
                     DRHSRILVLASGPLPELGPRSVVRDTWTMRDVLAEADRFASAEPDCDTVIYLRGDEPF
                     LDAGLTGRLLELHGRYRAEYTFADGYPPGF"
BASE COUNT          359 a          720 c          778 g          434 t
ORIGIN      
        1 cccgaaggcg gcagccatcc tggcggagtg ggcgtaggcc tccccggtca tgatggcgct
       61 gatagccgcg gcgaccgctc gtccctcggc cgagtcgtag ggcaggccca tcagcatgag
      121 gagcgtgccg aggttggcgt agccgaggcc gagcgtgcgg tagtcgtagc tctttctggc
      181 tatctccttg gaggggaact gggccatggc tacggatatc tcgagggtca gggtccataa
      241 tctgacggcg tggcggtaac gatccacgtc gaaggaccgg gcccgctcat catagaaggc
      301 ggtcagcttg agcgaggcca ggttgcaggc cgtgtcgtcg aggaacatgt actcggaaca
      361 ggggttcgag ccccgtatct cgccgtcggc ggggcaggta tgccagtcgt tgatggtcgt
      421 gtggaactgg agccccggat cggcgcactg ccacgcggac ttggctatgc ggtcccatag
      481 cgagcgggcc ttgatggtac gcagggtctt gccgcctcgg cgcatcgtca gcgcccagtc
      541 cttgcccttg gccacggctt ccatgaactc gttcgtgacc cggaccgagt tgttcgacga
      601 ctggccggag acggtgttgt aggcctcgcc ctcccagctg gcgtcgtaga ccggcccgtc
      661 gacgtccggg tcgccgtcgg cgatgcgcct gaggagctgg tagacgtagg agcccggtac
      721 gccggcgtcg agggcttcct tggcggccga gcgcagccac gggttggcgt cgatcgacag
      781 ccggtcggac gcgggcacgt cggacctggc cgcggcgtcg cgcatggcgc tcgagtactg
      841 cttgagcgcc ttgctccctg cgacgaggca ggcgaccttg tactcctcgc cggctttcca
      901 gtcgacgaac tgctcgatgt cgggatggtc ggcgtcgagg atgaccatct tggcggcgcg
      961 gcgggtcgtg ccgccggact tgatagccgc cgccgagcga tcggctatct tgaggaagga
     1021 caggagcccg ctcgagaagc cgccgcccga cagcggctcg cccgcgccgc gaattttcga
     1081 gaagttcgag ccggtacccg agccatactt gaacagcctc gcctcgcgct cgacgagatc
     1141 catgatgccg ccgtcgttga ccaggtcgtc ctgcacgtcc aggatgaagc aggcgtgagg
     1201 ctggggccgc tcgtaggcgt tcgtcgaggc gacgacctgg ccgctcttcg ggtcgacgta
     1261 gtagtggccc tgggccgggc cctcgaggcc gtacacggcg tgcaggccgg tgttgaacca
     1321 ctgcgggctg ttcggagcgc ccatctggtt ggccagcatg aagcacatct cgtcgtagta
     1381 agccttctcg tcggctttcg aatcgaagta gcccgattcc ttggcccaaa gcgtccaggt
     1441 gtaggccaag cgatggaaga cctggcgcgc gtcgtgctcg gacccgggca cgggggaggc
     1501 gccggccagg gcctgctgcg acgacggtac ccaggcgcgc caagcctcgg ccttgtcatc
     1561 gggcacgcct gccttgcgaa agtatttctg ggctatgatg tcggtggcta tctggctcca
     1621 ggtcgacggc acgatgacgc catccatccg gaagaccgcc ttgccgtcgg ggttccgtat
     1681 ctcggagact cgtttttccc atcggacgcc ctcgtacggg cccttgccgg cggtcgtgaa
     1741 cagtctgtct atcttcatga tgtcctcccc atgcgatcga taaatataca acttattgta
     1801 ttgcctggat attatactgc tatatagtgt atgtgtcgag gcctgtatta gcggctaacg
     1861 gcgagttgga tcgaggccga tactaagtat gactatggaa acactagcga taatcaacgc
     1921 gataggcttg agcgagcacg cgacctcccc ctgggccgat ggcccgagcg ccctcgagcg
     1981 ggcggttgcc ttcgccgagc gcctggctcc ggggcgcccc ggggccgata ggcattccag
     2041 gatactcgtg ctggcttcag gtcccttgcc ggaactcggg ccgcgttcgg tcgtccgcga
     2101 tacctggacg atgcgcgacg tactcgccga ggccgaccgc ttcgcgtccg ccgagcctga
     2161 ctgcgacacc gtgatctacc tgagaggcga cgagcccttc ctcgacgcgg ggctgaccgg
     2221 gcgcctgctc gagctgcacg ggcgctaccg cgccgagtac accttcgccg atgggtaccc
     2281 gcccggcttc t
//
LOCUS       DAOOLN010000210         2281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_58867_length_2281_cov_3.619946, whole genome shotgun sequence.
ACCESSION   DAOOLN010000210 DAOOLN010000000
VERSION     DAOOLN010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58867_length_2281_cov_3.619946"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1744
                     /locus_tag="PLW80_03530"
     CDS             <1..1744
                     /locus_tag="PLW80_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPB65605.1"
                     /translation="RRVNGPVIEAMGIGDARMLELVKVGESGLIGEIIKLSGNGATIQ
                     VYEDTTGVAPLDNVYGTGRPLSLELGPGLLGTIYDGIQRPLDALLALSGRFIERGVSA
                     EALDRTRLWAFEPRVAVGDEVSAGAVLGVVQETSRIEHRVCAPPGVSGRLVSVASAGE
                     YRVDETIAVVSGPDGERELSMMQRWPIREPRPVAQRKPLDIPLITGQRVIDTLFPLAK
                     GGTVAIPGGFGTGKTMTQHAIAKWCDADIIVYIGCGERGNEMTDVLNEFPKLVDPRTG
                     KSLMERTILIANTSNMPVSAREASVYTGVTLAEYYRDQGYHVAVMADSTSRWAEALRE
                     LSGRMEEMPAEEGFPAYLPTRIAEFYERAGYAETLSGADGSVTIIGAVSPPGGDFSEP
                     VTQHTKRFVRCFWGLDRQLANARHYPAISWLASYSEYLGDVRPWWEERVGPDWATARE
                     EIMELLQKEVRLLQVVKLVGPDALPDSQRFVIEVCSMFKNAFLQQSAFDDVDRYSTVE
                     KQLRMLQAIMAYWRRGQEAIKRGVTLHKLKRVKAVQDLVKMKFTIPNESPEEFDRLMA
                     RLERGIDQLEAIYA"
     gene            1737..>2281
                     /locus_tag="PLW80_03535"
     CDS             1737..>2281
                     /locus_tag="PLW80_03535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606825.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPB65606.1"
                     /translation="MRDEERRVLMSGREYVGAARIDGPLVFVRGTHPVGYRELVECVD
                     ADGNVRLGMVLDTSDDAVVVQVFEGTDGLTMPGTRTRFRGRPLTVGVSDRMLGRVFDG
                     LGRPVDGGPAPLADDEPDVNGRPINPTAREYPRDFIQTGISVIDGMNTLIRGQKLPIF
                     SGNGLPHNELAAQIARQAKLRG"
BASE COUNT          384 a          742 c          805 g          350 t
ORIGIN      
        1 gcgcagggtc aacgggccgg tcatcgaggc catgggcatc ggcgatgccc gtatgctcga
       61 gctggtcaag gtcggcgagt cgggcctgat aggcgagatc atcaagctgt ccgggaacgg
      121 ggcgacgatc caggtctacg aggacaccac gggcgtggcg ccgctcgaca acgtgtacgg
      181 caccggtcgg ccgctatcgc tcgagctcgg tccgggcctg ctcgggacca tctacgatgg
      241 catccagcgg ccgcttgacg cgctcctggc cctgtcgggc cgcttcatcg agcgtggcgt
      301 ctcggcagag gcgctcgacc gtaccaggct ctgggccttc gagccgcgcg tagcggtcgg
      361 cgacgaggtg tcagcgggcg cggtgctcgg cgtggtgcag gagacttcga ggatagagca
      421 ccgggtctgc gctcctcccg gcgtttccgg gcggctcgta tcggtggcgt cggccgggga
      481 gtaccgggtc gacgagacga tcgcggtcgt ctcggggccg gatggcgagc gtgagctgtc
      541 gatgatgcag cgctggccga ttcgcgagcc tcggccggtc gcgcagcgca agccgctcga
      601 cataccgctg atcaccgggc agcgcgtgat cgacacgctg ttcccgctgg ccaagggcgg
      661 caccgtggcc attcccggcg gcttcggcac cggcaagacg atgacccagc acgcgatagc
      721 caagtggtgc gacgccgaca tcatcgtgta catcggctgc ggcgagcgcg gcaacgagat
      781 gaccgacgtg ctcaacgagt tcccgaagct cgtcgacccg aggaccggca agagcctcat
      841 ggagcgcacg atactcatcg ccaatacctc gaacatgccg gtgtcggccc gcgaggcgag
      901 cgtgtacacc ggcgtgacgc tcgccgagta ctaccgcgac cagggctacc acgtggcggt
      961 catggccgac tcgacgtcgc gctgggccga ggcgttgcgc gaattgtcgg gtcgcatgga
     1021 ggaaatgccg gccgaggagg gcttcccggc ctatctgccg acgaggatcg ccgagttcta
     1081 tgagcgcgcc ggctacgcgg agaccttgtc cggggccgac ggctccgtga cgatcatcgg
     1141 cgcggtcagc ccgcccggcg gcgacttctc cgagccggtc acccagcaca ccaagcgctt
     1201 cgtgcgctgc ttctgggggc tcgaccgcca actggccaat gcgcgccact atccggccat
     1261 atcatggctg gccagctact cggaatacct cggcgacgtg cggccctggt gggaggagcg
     1321 ggtcgggccg gactgggcga ccgcgcgcga ggagatcatg gagctgctcc aaaaagaggt
     1381 gcggctcctg caggtggtca agctcgtcgg tccggacgcc ctgccggaca gtcagcgctt
     1441 cgtcatcgag gtctgctcga tgttcaagaa cgctttcctc cagcagagcg ccttcgacga
     1501 cgtcgaccgc tactccaccg tggagaagca gctacgcatg ctgcaggcca tcatggcgta
     1561 ttggcgccga gggcaggagg ccatcaagcg cggcgtgacg ctccacaagc tgaagcgggt
     1621 aaaggccgta caggacctgg tcaagatgaa atttaccata ccgaacgagt cgcccgagga
     1681 attcgaccgc ctgatggcca ggcttgagcg cggcatcgac cagctggagg ctatctatgc
     1741 gtgacgagga gaggcgggtc ctgatgtccg gccgcgagta cgtcggagcg gccaggatag
     1801 acgggccgct cgtgttcgtg cggggcaccc atcccgtcgg ctaccgcgag ctggtcgagt
     1861 gcgtcgacgc cgacgggaac gtgaggctgg gcatggtgct cgacacgagc gacgacgccg
     1921 tcgtcgtaca ggtgttcgag ggcaccgatg gcctgacgat gccgggtacc aggacgcggt
     1981 tccgcggcag gccgctgacc gtcggcgtgt ctgaccgcat gctcggccgc gtcttcgacg
     2041 gcctcggccg cccggtggac ggcggccccg cgccgctcgc cgacgacgag cccgacgtga
     2101 acggccgccc catcaacccg acggcccgcg agtatccacg cgacttcatc cagacgggca
     2161 tctcggtgat cgacgggatg aatacgttga tccgcggcca gaagctgccg atcttctcgg
     2221 gcaacggcct gccgcacaac gagctggccg cccagatagc cagacaggcc aagctgcgcg
     2281 g
//
LOCUS       DAOOLN010000211         2279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_58967_length_2279_cov_2.027878, whole genome shotgun sequence.
ACCESSION   DAOOLN010000211 DAOOLN010000000
VERSION     DAOOLN010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58967_length_2279_cov_2.027878"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2028)
                     /locus_tag="PLW80_03540"
     CDS             complement(<1..2028)
                     /locus_tag="PLW80_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HPB65607.1"
                     /translation="MKEAGYEALAADSGPAAVDEHGMAKAREARWQKRRFVVALATAL
                     PLLYVSMGHMIGLPLPPFLHPMHEPLNFALAQAALTLPALWAGRKFYTVGVKALLRRA
                     PNMDSLIAIGTGSAMAYSLWSTIQIALGDASAAGHLYFETVAVIIALILLGKYLEARS
                     KGKASEAIKRLMGLAPKTAVVVSESGDLELPVDEVAVGDVIRVRPGERVPVDGVVVEG
                     STSVDESMLTGESLPVEKSPGDKVSGATVNGQAMFTFRANAVGADTALARIVRLVEEA
                     QGSKAPIAALADRVSGVFVPVVVAIAFASAVAWLIGGQSFEFAMRVFVAVLTIACPCA
                     LGLATPTAIMVGTGRGAELGLLIKGGEALENAHRVTAVALDKTGTVTLGKPAVVEVLT
                     YGHLDEEAALALAASAERGSEHPLGAAIVREAEARSLALSSPDSLKALPGRGIEARLG
                     GAEAIVGNARLMEERGIDVGPAAADLERLAADGKTAMLVAAEGRLVGLVAVADIVRPT
                     SRAAIAALRARGVETTMITGDSRAVAEAIGREVGVDRVVAEVLPEDKSAAVRALQASG
                     KLVAMVGDGINDAPALAAADVGIAIGSGTDIAAESADIVLARGDLEDAVAALDLSRAT
                     MRVIRQNLFWAFGYNVLGIPVAAGLLHLFGGPLLNPMIAAAAMSLSSVSVVT"
BASE COUNT          336 a          819 c          782 g          342 t
ORIGIN      
        1 cgtgacgacc gacaccgacg acagggacat ggccgcggcg gctatcatcg ggttgagcag
       61 gggcccgccg aacaggtgga gcaggcccgc cgccaccggt atgccgagga cattatagcc
      121 gaaggcccag aacaggttct gcctgatgac gcgcatcgtc gcgcgggaca ggtcgagggc
      181 ggcgacggcg tcctcgaggt ctccgcgggc cagcacgata tcggccgact cggccgctat
      241 gtcggtaccc gaccctatcg ctatgcccac gtcggccgcc gccagggcgg gcgcgtcatt
      301 gatgccgtcc ccgaccatcg ccaccagctt gcctgaggcc tggagcgccc gcaccgcggc
      361 cgacttgtcc tcgggcagca cctccgcgac gactcggtcg actcctacct cgcggcctat
      421 cgcctcggcg acggctctcg agtcgccggt gatcatcgtc gtctcgacgc cgcgcgccct
      481 taaggccgcg atcgccgccc ggctcgtcgg cctgacgatg tcggcgaccg cgacgaggcc
      541 gacgaggcga ccttccgccg ctacgagcat ggcggtcttg ccatcggccg cgagcctctc
      601 aaggtcggcg gcggctggcc caacgtcgat cccgcgctcc tccatgagcc tcgcgttgcc
      661 gacgatggcc tcggcgccgc cgaggcgcgc ctcgatgcct cggccgggca gggccttgag
      721 gctatcgggc gatgatagcg cgagcgaccg ggcttccgcc tcgcggacga tcgcggcgcc
      781 gagcgggtgc tccgatcccc tctcggccga cgcggccagg gccaacgcgg cttcctcgtc
      841 caggtggccg tacgtgagga cctcgacgac ggccggtttg ccgagcgtca ccgtaccggt
      901 cttatcgagc gctacggccg tcacgcgatg ggcgttctcg agcgcctcgc cgcccttgat
      961 cagcaggccg agctcagcgc cgcgcccggt gccgaccatg atggccgtcg gcgtggccag
     1021 cccgagcgcg caggggcagg ctatcgtcag tacggccacg aacacccgca tcgcgaactc
     1081 gaagctctgt cccccgatca gccacgcgac cgcggaggcg aaggctatgg cgacgacgac
     1141 cggtacgaag acgcccgata cccggtcggc gagcgcggct atcggggcct tggagccctg
     1201 ggcctcctcg actaggcgga cgatgcgggc cagggccgtg tcggcgccga cggcgttcgc
     1261 cctgaaggtg aacatggcct ggccgttgac ggtggcgccg gataccttgt cgcccgggct
     1321 cttctcgacg ggcaggctct ctccggtcaa catggactcg tcgacgctgg tcgagccttc
     1381 gacgacgacg ccgtcgaccg gcactcgttc gccggggcgc acgcggatca cgtcgccgac
     1441 ggccacctcg tcgaccggca gctccaggtc gcccgattcc gacacgacca ccgcggtctt
     1501 gggcgccagg cccattagcc gcttgatggc ctccgatgcc ttgcccttgg accgggcctc
     1561 gaggtacttg ccgagcagga tcagggcgat gatgacggcg accgtctcga agtacaggtg
     1621 tccggccgcg gaggcgtctc cgagggcgat ctgtatggtc gaccagaggc tgtaggccat
     1681 cgccgagccg gtgcctatcg cgatcaggct gtccatatta ggcgctcgcc tgagcagggc
     1741 cttgacgccg acggtgtaga atttcctccc ggcccatagc gccggcaggg tcagcgccgc
     1801 ctgggccagc gcgaagttca ggggctcgtg catgggatgc aggaagggcg gtagcggcaa
     1861 tccgatcatg tggcccatcg atacgtagag gagtggaagg gccgtcgcta gcgcgacgac
     1921 gaaacgccgt ttttgccaac gcgcttcgcg cgccttggcc atgccgtgct cgtcgacggc
     1981 ggccgggcct gagtcggccg ccagcgcctc gtagcccgcc tccttcacgg ccagcttgat
     2041 ggacgacagg cgggaccgac gggtcccagg acaccgacag ccgctcggag gcgaaattca
     2101 ccgaggcgct cgagacgccg ggtaccagtg acaccgccct ctcgacggcc gcggcgcagg
     2161 cggcgcagct catgccgcct atcggcacgg acgcgctctt gaccgccgtc ggtagcacgg
     2221 cttcgtagcc ggcctccgct atcgccgcgg cgatggccgc cagccctgtc gcgccctcg
//
LOCUS       DAOOLN010000212         2274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_59148_length_2274_cov_2.611086, whole genome shotgun sequence.
ACCESSION   DAOOLN010000212 DAOOLN010000000
VERSION     DAOOLN010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59148_length_2274_cov_2.611086"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..800
                     /locus_tag="PLW80_03545"
     CDS             201..800
                     /locus_tag="PLW80_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002678721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HPB65608.1"
                     /translation="MYDFIRCEVPDCRATALTGYSFCADHCHDPSAVAASMAALIRSS
                     SSVRSVNASGLRFEGLDLSGKSFYACSFSGTVLKNVAFTGCTFRMCFFDFCSADSCDF
                     SGIDAQFCSFAGARFLNISFERSELVHNNFDGVRTNDSTFNNSDLYNSRFIQSEFEKT
                     DFVDCNLKRAFIIPSKEIDVSWRLSNTQEAILGVDRLEE"
     gene            797..1387
                     /locus_tag="PLW80_03550"
     CDS             797..1387
                     /locus_tag="PLW80_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB65609.1"
                     /translation="MTVFFHYSLYGFANVYLVGNDETREAVVVDPAAFTIGLLDFIEG
                     KGYTPKAVLVTHSHAHHVDGLRTLLRIYDAEVYSSNAMIGEVKCRTVRDAERFVACGL
                     EVEALSVPGHSPDSIVYRIEKLLFTGDALHAGLVGKTASQYGSRLLKEQLSRKVLNQD
                     DDCIVLPGHGPPSTIGAEKLYNLGWRAVRADGTPRY"
     gene            1482..2015
                     /locus_tag="PLW80_03555"
     CDS             1482..2015
                     /locus_tag="PLW80_03555"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HPB65610.1"
                     /translation="MKRIRIIAVAMLAMLALGAVSAWAADDILGLWKIIDDKTGKPNA
                     YVMLYMNGGKLYGRMIATISLDTGLIDDTLATKKFRADALAGDPPNCGLDFVYELQDK
                     GKEWKGSIIDPADGKVYDCSIKREGSKLTVRGSLKGTGGLLGRNQTWLLASASEMPAG
                     FALPDPRTFAPVIPRKK"
     gene            complement(2026..>2274)
                     /locus_tag="PLW80_03560"
     CDS             complement(2026..>2274)
                     /locus_tag="PLW80_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65611.1"
                     /translation="ASAPIIRFDKPPKGSGDLSSALFLGYYLIGGGVGEAIEKTLDAL
                     YSVLESSASGGGDTLAIVAAQDAIAEPGRRFTATAPTG"
BASE COUNT          441 a          726 c          685 g          422 t
ORIGIN      
        1 agacggcgcg gaagccaatg gttccttggg gtagctggga gccgcaatac gagcacatga
       61 gcgcagtgta cgcccgagac gaggaatatt caagttacgg atcgattata tagaacgcgg
      121 cttgagcgca gacccgtttt aggctatcct cgctcgcagg gcttagcgcc ctgcgagcgg
      181 ctatcgagga ggccctgccg atgtacgatt tcatacgatg cgaggtcccc gattgccgcg
      241 ccacggccct gacgggttac tctttctgcg ccgatcattg ccatgacccg tcggccgtcg
      301 ccgcctcgat ggcggccctg atccgttcct cctcgtcggt caggagcgtc aatgcctccg
      361 gcctccgctt cgaggggctg gacctatcgg gcaagtcgtt ctacgcgtgc tcgttctccg
      421 ggaccgtact caagaacgta gcgttcaccg gctgtacctt caggatgtgc ttcttcgact
      481 tctgctcggc cgactcctgc gacttctcgg ggatagacgc ccagttctgc tccttcgccg
      541 gcgccaggtt cctgaatatc tcgttcgagc gttccgaact cgtgcataac aacttcgacg
      601 gcgtcaggac caacgactcg accttcaata attcggacct gtataactcc aggttcatcc
      661 aatccgaatt cgagaagacg gatttcgtcg actgcaacct gaagcgggcg ttcatcatcc
      721 cgtccaagga gatcgacgtg tcgtggcggc tgtcgaatac gcaggaagcc atccttggcg
      781 tggacaggct cgaagaatga ccgtcttctt ccactactcc ctgtacggct tcgccaacgt
      841 ctacctcgtc gggaacgacg agacgaggga ggccgtcgtc gtcgacccgg cggcctttac
      901 gataggcctg ttggatttca tcgagggcaa aggctatacg cccaaggcgg tgctcgtcac
      961 ccatagccac gcccaccatg tcgacggcct gcgcacgctg ctgcgcatct acgacgccga
     1021 ggtctactcg agcaacgcga tgataggcga ggtcaagtgc aggacagtcc gggacgccga
     1081 gcgtttcgtc gcctgcgggc tcgaggtaga ggccttgtcc gtccccggcc actcgcccga
     1141 ttccatcgtc taccggatcg agaagctatt gttcactggc gacgcgttgc acgccgggct
     1201 ggtcgggaag actgccagcc agtacggcag ccgactcctc aaggaacagc tgtcgaggaa
     1261 ggtgctcaac caggacgacg actgcatcgt gctaccgggc cacggcccgc cttccacgat
     1321 cggcgccgag aagctctaca atctggggtg gcgcgccgtc cgcgccgacg gaacgccgag
     1381 atactaactt ctctttacag ggcgtcggag ccatgcctat actgctaact cacagcacgc
     1441 ggcgaggccc tagccgccgc gggaaacgcg agggaaaacg tatgaagagg ataaggatta
     1501 ttgccgtcgc catgctcgcc atgctggctc tcggcgcggt ctcggcctgg gctgcggacg
     1561 atatccttgg cctatggaaa atcatcgacg acaagaccgg caagccgaac gcctacgtca
     1621 tgctctacat gaacggcggc aagctgtacg gccgcatgat agccaccatc agcctcgata
     1681 ccgggctcat cgacgatacg ctggccacga agaaatttcg ggcggacgcg ctagccggag
     1741 acccgccgaa ctgcggcctg gatttcgtct acgagttgca ggacaagggc aaggaatgga
     1801 aaggctcgat catcgaccca gccgacggca aggtgtatga ttgctccatc aagcgcgaag
     1861 gctcgaagct gacggtacgc ggctcgctca agggcaccgg cggactgctc ggcaggaacc
     1921 agacctggct gctcgccagc gccagtgaga tgcccgccgg tttcgccttg cctgacccgc
     1981 ggaccttcgc gccggtcatc ccccgcaaga agtaagctcc gcggcctagc cggtcggggc
     2041 ggttgccgtg aagcgccgcc ccggttcggc tatagcatcc tgcgcggcga caatcgccag
     2101 ggtgtcgccg ccgcccgagg cgctcgactc gagcaccgag tacagcgcgt cgagcgtttt
     2161 ctcgatcgcc tcacctaccc cgcccccaat caggtagtaa ccgaggaaca gggcgctcga
     2221 caggtctccc gaccctttgg gaggcttatc gaagcggatg atgggcgcgg acgc
//
LOCUS       DAOOLN010000213         2264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_59533_length_2264_cov_3.679946, whole genome shotgun sequence.
ACCESSION   DAOOLN010000213 DAOOLN010000000
VERSION     DAOOLN010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59533_length_2264_cov_3.679946"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLW80_03565"
     CDS             complement(<1..233)
                     /locus_tag="PLW80_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPB65612.1"
                     /translation="MHVLMVTSEATPYAKSGGLADAVSALSKALRRLGHDVRVVMPRY
                     GSVPVEGLSRREGELVVEMGGSVERALVYKGLLP"
     gene            complement(356..>2264)
                     /gene="recG"
                     /locus_tag="PLW80_03570"
     CDS             complement(356..>2264)
                     /gene="recG"
                     /locus_tag="PLW80_03570"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPB65613.1"
                     /translation="NCRVAVVAHDWFGFGRMRTLKVWVEDERGTRAALVCFNRPFLER
                     SLPVGSSLKLYGRFDYRYSELQSSAFDAEPVDEVADGLPDGVRPVYPLTEGLGQSTMR
                     KLVTGALARWGAVDDELPPSVRARRGLLRKAEALRALHAPSTPAETVEAKRALAYEEL
                     FYLQVMIARRAASRRSDERPREPPPAGLALALEERLPFELTADQKAAVADIVADLSGP
                     YPMARLLQGDVGSGKTLVAFFAALYAIASGGQVAMMAPTELLARQHADTAARLLEPLG
                     VRLAFLSGNVADAARRPLLAALSRGEVDLVIGTHALFSGDVEYRNLSLAVVDEQQRFG
                     VLQRLALGAKGRKPDMLMMTATPIPRTLALTAFGDLAISTIRTMPKGRLPVVTHLAKD
                     GNEAKVYEFVRRELAAGRRAYFVYPLVDRSDRLALKDAEAMFERLSRDVYPEYQGGLI
                     HARLPEEKKRQTMADFAAGRLSFIVATSVVEVGVDVPEATCMVVEHAERFGLAALHQL
                     RGRVGRSDLQSYCFLVWSDGRAEDGARLSEDGKRRVMAMKSTTDGFEIAEEDLRIRGP
                     GEITGTTQAGALRLAFADPVRDAELLEAAREDAAALLATDPGLVGQEGGIVRAVLERA
                     SPFSERTAATG"
BASE COUNT          353 a          775 c          800 g          336 t
ORIGIN      
        1 ggcaggaggc ccttgtagac gagcgctcgc tcgaccgagc cccccatctc gacgacgagc
       61 tccccctccc gccgagaaag cccctcgacg ggaaccgagc cgtagcgggg catcacgacg
      121 cggacatcgt gcccgaggcg gcgcagggcc ttggataagg ccgagacggc gtccgcgagc
      181 ccgccagact tggcgtaggg cgtcgcctcg ctggttacca taagcacgtg cacaggccgg
      241 ctcctcgtat gcgcaaaacc tgcgcggtat tagtcgttct cagtgcttaa caaacaagca
      301 gtatactaag atacgggcct gcgggcaacg ccgcgacgga tggcgccagc cgcattcagc
      361 cggtggcggc cgtacgctcc gagaaggggc tggctcgctc gagcaccgcg cggactatcc
      421 cgccctcctg cccgacgagc cctggatctg ttgccaggag ggccgcggcg tcctctctgg
      481 ccgcctcgag cagctcggcg tcgcgcactg ggtcggcgaa ggccagcctc agggcgccgg
      541 cctgggtcgt gccggtgatc tcgccggggc ctcggatgcg caggtcctcc tcggctatct
      601 cgaagccatc cgtcgtcgac ttcatcgcca tcacgcggcg cttgccgtcc tcggacaacc
      661 tggccccgtc ctccgcccgg ccgtcggacc agacgaggaa acagtacgac tgcaggtcgc
      721 tccgccctac cctgccgcgt agctggtgca gtgccgcgag cccgaagcgc tcggcgtgct
      781 cgacgaccat gcaggtagcc tcggggacgt cgacgccgac ctcgacgacg ctcgtggcga
      841 caatgaacga tagccggccg gcggcgaagt cggccatcgt ctggcgcttc ttctcctcgg
      901 gtagcctggc gtggatcagg ccgccttggt actcggggta gacgtcgcga gacaggcgct
      961 cgaacatggc ctcggcgtcc ttcagggcca gccggtcgga acggtcgacc agcggataga
     1021 cgaagtaggc gcgcctgccg gcagccagct cgcgccgcac gaattcgtag accttggcct
     1081 cgttgccgtc tttggccagg tgcgtgacga cgggcagtcg gcccttcggc atcgtgcgta
     1141 tcgtcgatat cgcgaggtct ccgaaggcgg tcagcgcgag ggtcctcggg atgggcgtcg
     1201 cggtcatcat gagcatgtcc ggcttgcgtc ccttggcgcc gagcgccagc cgctggagca
     1261 cgccgaaacg ctgctgctca tcgacgacgg ccaggctgag gttccggtac tcgacgtcgc
     1321 ccgagaacag ggcgtgcgtg ccgatgacga ggtccacctc gcctcgggac agggcggcca
     1381 ggagcggtcg cctcgcggcg tcggcgacgt tgcccgacag gaaggcgagc cgtacgccga
     1441 gcggctcgag cagcctggcg gcggtgtcgg cgtgctgcct ggccaggagt tcggtcgggg
     1501 ccatcatcgc gacctggccg cccgaggcta tggcgtagag cgccgcgaag aacgcgacga
     1561 gggtcttgcc cgaacccacg tcgccctgga ggagcctggc catcggatag gggcccgata
     1621 gatcggcgac gatgtcggcg acggcggcct tctggtcggc cgtcagctcg aacggtaagc
     1681 gctcctcgag ggcgagcgcc aggccggccg gaggcggctc acgaggccgc tcgtcggagc
     1741 gcctggacgc ggctcgcctg gctatcatca cctggagata gaacagctcc tcgtaggcga
     1801 gggcgcgctt ggcctcgacc gtctcggccg gcgtcgaagg ggcgtgcagg gcgcgcaggg
     1861 cctcggcctt gcgtaagagg ccgcgccgcg cgcgtaccga cggtggcaat tcatcgtcca
     1921 ccgctcccca gcgcgccagc gctccggtta cgagcttgcg catcgtcgac tggccgaggc
     1981 cctcggtcag cgggtagacg ggcctgacgc cgtccggaag cccgtcggcc acctcgtcta
     2041 ccggctcggc gtcgaaggcc gacgactgca gctcggagta gcggtagtcg aagcggccgt
     2101 acagcttcaa ggaagagccg accggcaggc tgcgctcgag gaagggccgg ttgaagcaga
     2161 cgagggcggc cctcgtcccg cgctcgtcct cgacccagac cttgagggta cgcatgcggc
     2221 cgaagccgaa ccaatcgtgg gcgaccacgg cgacgcggca gttg
//
LOCUS       DAOOLN010000214         2262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_59621_length_2262_cov_3.588582, whole genome shotgun sequence.
ACCESSION   DAOOLN010000214 DAOOLN010000000
VERSION     DAOOLN010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59621_length_2262_cov_3.588582"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1982)
                     /gene="pepF"
                     /locus_tag="PLW80_03575"
     CDS             complement(177..1982)
                     /gene="pepF"
                     /locus_tag="PLW80_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HPB65614.1"
                     /translation="MANANEIPARRDVPAEHRWNLSRLSKDEASWEADLKSLEGMVGR
                     VAAFRGTLASSSDSLYAALSFYKEYSMLDERLGYYAHLRQTEDEGDSSSRGRMARYMM
                     AATKASGEWSWLTPELQRVPDASMKAWMAEERYAEFRVYLSKILRYKPHVLSAEEERL
                     IALASDAATTPQEAFSVLTNVDLDFGVVDTPEGPRPLSQSTFSSFMHSPDRKVRREAY
                     ARFYAEYDAHKNTIAQLYAGQCKLDSYQSTVRKFTSSRQRELFADDVPESVYDNLVST
                     VNANLPVLHEYYAMRKKALGLDELRHYDVYVPLVGQVKVEHSWEEAVDVITEALAPLG
                     SEYVSTLRSGLLGGWADRYENKGKRSGAFSAGSFSGDPYILMNYKSDVIRDVFTMVHE
                     GGHSMHSYYAASSNPFMHYNYTIFEAEVASTFNEALLFDYMMRTAGSKELKTYLLASR
                     VDDILATLFRQTMFAEFEHKAHAMLESGQPLTVDALRGEYRTLLEKYFGPEMVFEEAS
                     DLEGLRIPHFYNSYYVYKYATGISASIALSKKVLSGEKGATENYYKFLRSGGSRFPIE
                     ALKVAGVDMSSPAPVEAACAEFKRLVGELKAALGV"
     gene            complement(2064..>2262)
                     /locus_tag="PLW80_03580"
     CDS             complement(2064..>2262)
                     /locus_tag="PLW80_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65615.1"
                     /translation="AEALAADGALPAWASPSAPGSRVPATWRVLHGPDDRVGLYDDAA
                     FELVARFLERLAALRVPAMIP"
BASE COUNT          362 a          696 c          798 g          406 t
ORIGIN      
        1 cggcgtcgcc acgctcgcag gttaacggcg gcgtcgccac gctcgcaggt caacggcggc
       61 gaggcctgca taaaatcccg agcctcgggg cggcgcgtcg agtaaacgct ccgccccctt
      121 tacgaatgac tctttcctta ctcttttcct cttcatccga cttcgttgtg ccggccctac
      181 acgcccagcg cggccttgag ctcgccgacg aggcgcttga actcggcgca ggcggcctcg
      241 acgggggcgg ggctcgacat gtcgacgccg gccaccttga gggcctcgat cgggaagcgc
      301 gagccgccgg agcgcaggaa cttgtagtag ttctcggtgg cgcctttctc gccggacagc
      361 accttcttgg acagagctat cgaggcggag atgccggtgg cgtacttgta gacgtagtac
      421 gagttataga agtgcggaat gcgcaggccc tcgaggtcgc tggcctcctc gaagaccatc
      481 tccggcccga agtacttctc gagcagagtg cggtactcgc cgcgcagggc gtcgacggtc
      541 aggggctggc cggactcgag catggcgtgg gccttgtgct cgaactcggc gaacatggtc
      601 tgcctgaaca gggtggccag gatgtcgtcg acccggctgg cgagcaggta ggtcttgagc
      661 tccttggaac cggcggtcct catcatgtag tcgaacagca gggcctcgtt gaaggtgctc
      721 gcgacctcgg cctcgaagat cgtatagttg tagtgcatga aggggttgga cgacgcggcg
      781 tagtacgagt gcatcgagtg gccgccctcg tggaccatgg tgaacacgtc ccggatcacg
      841 tcgctcttgt agttcatcag gatgtagggg tcgccggaga acgagccggc cgagaaggcg
      901 ccggagcgct tgcccttgtt ctcatagcga tcggcccaac cgccgaggag gccggagcgc
      961 agggtggaga cgtactcgct cccgagcggg gccagggcct cggtgatcac gtcgacggcc
     1021 tcttcccagc tatgctcgac cttgacctgg ccgacgaggg gcacgtagac gtcgtagtgg
     1081 cgcagctcgt cgagcccgag cgccttcttg cgcatagcgt agtactcgtg gaggacgggc
     1141 aggttggcgt tgacggtgga cacgaggttg tcgtacacgc tctcgggcac gtcgtcggcg
     1201 aacagctcgc gctgccggct cgaggtgaac ttgcggacgg tggactggta cgagtcgagc
     1261 ttgcactggc cggcgtagag ctgggctatg gtgttcttgt gcgcgtcgta ctcggcgtag
     1321 aagcgtgcgt aggcctcgcg gcgcaccttc cggtcggggc tgtgcatgaa ggacgagaag
     1381 gtcgactggg acagcggcct cgggccctcc ggggtatcga cgacgccgaa gtcgaggtcg
     1441 acgttggtca gcacggagaa ggcttcctgc ggggtggtcg ccgcgtccga ggcgagggcg
     1501 atcaggcgct cctcctcggc cgacagtacg tggggcttgt agcgcaggat cttggacagg
     1561 tagacgcgga actcggcgta gcgttcctcg gccatccagg ccttcatcga ggcgtcgggg
     1621 acgcgctgca gctcgggggt cagccagctc cactcgcccg aggccttcgt ggcggccatc
     1681 atgtagcggg ccatgcggcc gcgcgagctc gagtcgccct cgtcctcggt ctgcctgagg
     1741 tgggcgtagt agccgagcct ctcgtcgagc atcgagtact ctttatagaa ggacagggcc
     1801 gcgtacagcg agtccgacga cgaggcgagg gtgccgcgga aggccgcgac gcggccgacc
     1861 atgccctcga ggctcttgag gtcggcctcc caggaggctt cgtccttcga cagcctggac
     1921 aggttccagc gatgctccgc gggtacgtcc ctgcgggccg ggatttcgtt tgcgttcgcc
     1981 atgtatatca ctccttttta gtggttccgt tcgtagtacc gacgcgtcac tataccggat
     2041 tcgaaaagcc gatccaagcg gcctcacggt atcatcgccg gtacgcgcag cgccgccagc
     2101 cgctcgagga agcgggctac cagctcgaag gcggcatcgt cgtacagccc gacgcggtcg
     2161 tccggcccgt ggaggacacg ccaggtcgcc ggtacgcggc tgcccggcgc cgaaggggag
     2221 gcccaggccg gcagagcgcc atcggccgcg agggcctcgg cc
//
LOCUS       DAOOLN010000215         2258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_59800_length_2258_cov_2.529732, whole genome shotgun sequence.
ACCESSION   DAOOLN010000215 DAOOLN010000000
VERSION     DAOOLN010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59800_length_2258_cov_2.529732"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PLW80_03585"
     CDS             complement(<1..545)
                     /locus_tag="PLW80_03585"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPB65616.1"
                     /translation="MNGSALSQYKGRRIAVGLSGGVDSSTALALLAEAGAHAFGLSMR
                     IWRPGAAPIAGVGDACYGPGEIEDVAAAESLCAGLGVPYRSLDLSAEYEARILDYFKR
                     EYRAGRTPNPCVRCNQEMKFGFLLERARSAGLDFELFATGHYARIDSRAGRPTLRRAA
                     DETKDQSYFIHRLSPATLSGVV"
     gene            709..1764
                     /locus_tag="PLW80_03590"
     CDS             709..1764
                     /locus_tag="PLW80_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65617.1"
                     /translation="MTIWRRAAAACAILAVAPLGAAAAPAAAVALDAFPDYLSVRSEF
                     LSAAITAAPAVALAFKPAYRETPAGRIRVSVERDGASFYVLFQRERDGGYPIGSAGNM
                     VIQRSAVTGYVTGVIWYLSDDGTSYVGLTPKNERTVVDYVVAGSLSRGDYALSRLVYQ
                     VFTGSFKAFHDATRGALDWSLVFGQPGPEASETFASALLAGGPSRAAKALLAAADDFT
                     AIGAYLGSVGRDGYEPEELREPRFAKAASFDDPRRPILRPIRAWSDDQGLPIEAAMAT
                     ILAAPPGGAYIALVGGVSDRPPRKLVVVPYRNGRGAYTLASFDADDSSAVDLAALVGS
                     MAGASVRLFSVPLPGTI"
     gene            1995..>2258
                     /locus_tag="PLW80_03595"
     CDS             1995..>2258
                     /locus_tag="PLW80_03595"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPB65618.1"
                     /translation="MDGLLIGFTSVSWQQGVMYLVGAALIILAVTKEYEPMLLLPIGF
                     GAILVNLPLSVVWEHEGAAGFLKVLYDGGISNELFPILIFIAVG"
BASE COUNT          339 a          778 c          733 g          408 t
ORIGIN      
        1 accacgccag acagggtcgc gggactcagg cggtgtatga aatagctctg gtccttggtc
       61 tcgtcggccg cgcgcctgag cgtcgggcgg ccggcccggc tatcgatcct cgcgtagtgg
      121 ccggtggcga acagctcgaa gtcgaggccg gccgagcgcg cccgctcgag caggaagccg
      181 aatttcatct cctggttgca gcgcacgcag gggttgggcg tacggccggc ccggtactcg
      241 cgcttgaagt agtcgagtat cctggcctcg tactcggccg ataggtcgag cgagcggtac
      301 ggcacgccga gcccggcgca gagcgactcg gccgcggcca cgtcctcaat ctcgccgggt
      361 ccgtagcacg cgtccccgac gccggctatc ggagcggcgc cgggcctcca tatcctcatc
      421 gacagcccga aggcgtgggc gccagcctcc gcgagtaggg ccagggcggt cgaagagtcg
      481 acgccgcccg acaggcctac cgctatgcgt cggcccttat actgactgag tgcgcttccg
      541 ttcatctcct ggatactagc ccgaagcggg cgaggcggac aagctggccg tatcggcccg
      601 cggcgtaacc atccgcgcgt attcgccttt tattgccatt gtggtatact tgcctgcgtt
      661 gaccaattcc gctgaagtcc tctttagcaa tcaaacggag catgactcat gactatatgg
      721 agacgcgcgg cagccgcctg cgcgatactt gccgtcgccc ctctcggcgc tgccgccgcg
      781 ccagcggccg ccgtagccct ggacgccttc cccgactacc tgtcggtcag gtccgagttc
      841 ctatcggccg cgataaccgc cgccccggcc gtagccctgg ccttcaagcc cgcctatcgc
      901 gagactccgg ccggccgtat ccgcgtctcg gtcgagcgcg acggcgcgtc cttctacgta
      961 ctcttccagc gcgagcgcga cgggggctac ccgatcggct cggcgggcaa tatggtcatc
     1021 cagcgatccg cggttacggg ctacgtgacc ggcgtgatct ggtacctgag cgacgacggg
     1081 acgagctacg tcggcttgac tcccaagaac gagcgcaccg tcgtcgacta cgtcgtggcc
     1141 gggtccctgt cgcgcggtga ctatgcgctg tctcgcctgg tctaccaggt gttcaccggc
     1201 agcttcaagg ctttccacga cgcgacgagg ggcgccctcg actggtcgct cgtgttcggc
     1261 cagcccggcc ccgaggcgtc ggagaccttc gcctccgccc tcttggccgg cgggccaagt
     1321 agggcggcca aggcactcct cgcggccgcc gacgatttta ccgcgatcgg agcctaccta
     1381 ggctcggtcg gccgcgacgg ctacgagccc gaggaactcc gcgagccccg gttcgccaag
     1441 gcggcctcgt tcgacgatcc gcgcaggccg atcctaaggc ccatccgcgc gtggagcgac
     1501 gaccagggcc tgcctatcga ggccgccatg gctacgatac tggcggcccc gcccggcggc
     1561 gcgtacatag cgctcgtcgg gggcgtctcc gaccggccgc cgcggaagct ggttgtggtg
     1621 ccctatcgga acggtcgtgg cgcctacacg ctagcctcct tcgatgccga cgattccagc
     1681 gccgtcgacc tggccgccct cgtaggttcc atggccggcg cgtccgtacg gctattcagc
     1741 gtcccgttac ccggaacgat atgattctgt tgacagatac cgaatgaggg cgtagcctcg
     1801 agtcagagtc aggctgcggc ccgggggggc gggacggcga gcctggcccg gggtagcacc
     1861 ccgactccat gaaacctcct ttccatgggg ccccccgccg cccgcgccta gcgcgggcgg
     1921 cttcgtcatc gccctccttg cctccgcggc gcgtaccgac tactatgtac ccatcgaatc
     1981 gggaaaggcg gattatggac ggtctactca taggcttcac ctcggtgagc tggcagcagg
     2041 gcgtgatgta tctggtcgga gcggcgttga tcattctggc cgttacgaag gaatatgagc
     2101 cgatgctgct cctgcccata ggcttcgggg ccattctcgt caacctgccc ttgtcggtgg
     2161 tgtgggagca tgagggcgcg gccgggttcc tcaaggtgct ctacgacggc ggtatctcca
     2221 acgagttgtt ccccatcctc atcttcatcg cggtcgga
//
LOCUS       DAOOLN010000216         2257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_59835_length_2257_cov_2.471844, whole genome shotgun sequence.
ACCESSION   DAOOLN010000216 DAOOLN010000000
VERSION     DAOOLN010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59835_length_2257_cov_2.471844"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PLW80_03600"
     CDS             <1..602
                     /locus_tag="PLW80_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65619.1"
                     /translation="DQASSKLKLIYSTDAIDASTPATVPSWSDPVIIDNLYSGSNVSL
                     YVENDGNAGTPDPIHLAYHDTSNADLRYTYLPSYDGTPTSVSVDSYNSVGTLTEVKSY
                     GGIPYIVYYNNSENGTRDSIKISIFRGTPGAITAGVNESGNVTGDWDSITIPANDIPV
                     GGLPDFNYVMLGFRSDGSPVIGYLSDDIEWARILPELAD"
     gene            679..1683
                     /locus_tag="PLW80_03605"
     CDS             679..1683
                     /locus_tag="PLW80_03605"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPB65620.1"
                     /translation="MGKRGFVLTAVVAACWLLAVGVMARAVSPEPPPPVEPGATVASH
                     TPDDTRRQRRISAAAAALRPEALAGVSAGESKARARALASRALAAGLEPAATVARALP
                     GSTWVQARLVMRRDEENLRLDTALDNAYGRSYAAIRAGLAETRATAEAVEAALGELWR
                     VYYDDARTDGSRGAPLVSPLARRDQWIPGTRALRASHQYALDIFFTSLSRDGAEEKGP
                     TVVSMSRGVVVSAAGDWVGGDRPSAYVSGGLSPKAGNGVVVYDPSTGRYYAYFHLSDV
                     AVRAGQFVEPGQELGRGGNTGVNARRRGHGGHLHLEIHEADGSALSSFSIRDLILSVR
                     "
     gene            1787..>2257
                     /locus_tag="PLW80_03610"
     CDS             1787..>2257
                     /locus_tag="PLW80_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65621.1"
                     /translation="MKRIAIVILLALVAFGAWAQDLGDFQNGFTGFASALAPTLSYNA
                     TAGNNWSDAYIGNLPHFGIGAAVGFTSVPIGELTTLIGTIASGVEIPAALSQLGLPVP
                     AAALSAKIGGFILPFDLGLKAMMLTPEMTASLDAMGFSFDYTLFGGNVRYALLKQ"
BASE COUNT          401 a          763 c          699 g          394 t
ORIGIN      
        1 acgaccaggc gagctccaag ctaaagctga tctactcgac tgatgccata gacgcgtcga
       61 ctccagcgac tgtgccctca tggagtgatc ccgtcatcat tgacaatctc tattccggct
      121 ccaatgtttc gctgtatgtc gagaacgacg ggaacgctgg gacgcccgat ccgattcacc
      181 tagcgtacca cgacacctct aacgccgatc ttagatatac gtatctcccg tcatacgacg
      241 gaacgccgac gagcgtcagc gtcgactcgt acaactcggt tggaaccctc accgaggtca
      301 agagctacgg aggcatacct tatatcgtgt attacaacaa ttcagagaat ggcactagag
      361 attctatcaa aatatccata tttaggggca cgccaggggc tataacggct ggcgttaacg
      421 agtcaggcaa cgttacgggg gactgggaca gtattacgat accggccaac gatataccag
      481 tcggaggatt gcctgatttt aactacgtca tgctaggctt cagaagcgat ggatcacccg
      541 tcataggcta tctatcagac gacattgaat gggccaggat acttcccgag ctggcagact
      601 aaccccttcc cttgaccccg ctccctcccc cggctagact gacaggccgg ccggaggggc
      661 ggcgggaagg cgggcttctt gggcaagcgc gggttcgtac taacggccgt cgtggcggcg
      721 tgctggctgc tcgccgtcgg ggtgatggcc cgcgcggtgt cccccgagcc tccgcccccg
      781 gtcgagcccg gagcgaccgt cgccagccat acacccgacg acacgcggcg gcagcggagg
      841 atctcggccg ccgccgcggc cctgcggcca gaggcgctcg cgggcgtatc ggccggcgag
      901 tcgaaggccc gggcgcgggc cctcgcgagc agggccttgg cggccgggct cgagccggcc
      961 gctacggtcg cccgcgccct gccgggctcg acctgggtcc aggcccgcct ggtcatgcgc
     1021 cgcgacgagg agaaccttag gctggacacg gccctggata acgcctacgg aaggtcgtac
     1081 gcggccatcc gcgccggtct cgcggagacc cgggccacgg ccgaggcggt cgaggccgcc
     1141 ctaggcgagc tatggcgcgt ttattacgac gacgctcgga ccgacgggag ccgcggagcg
     1201 ccgctcgtct cgccactggc ccgccgcgat cagtggatac ccggaaccag agccctccgc
     1261 gcgtcccacc agtacgccct cgacatcttc ttcacgagcc tgtcgcgcga cggcgccgag
     1321 gagaaaggcc cgacggtcgt cagcatgagc cgcggcgtcg tcgtctcggc cgcgggcgac
     1381 tgggtcggcg gcgaccgtcc ctcggcgtac gtctcgggcg gcctgtcgcc gaaggccggc
     1441 aacggcgtcg tggtctacga cccgtcgacc ggccgctact acgcgtactt ccacctgagc
     1501 gacgtagcgg tacgggccgg ccagttcgtc gaaccggggc aggaactagg ccgcggaggc
     1561 aacaccggcg tcaacgctcg caggcggggc cacggcggcc acctgcacct ggagatccac
     1621 gaggccgacg gctcggccct atcgtcgttc tccatccgcg atctcatcct atccgttcgc
     1681 tgatcgtgtc cattcgttga acaactacgt gatttctcac cgatacggca gtatagtagt
     1741 ctagtgcgcc aaggttactg tcctcggcgc gccttgagga ggtaccatga aacggatcgc
     1801 catcgtcata ctgctcgccc tcgtagcctt tggcgcctgg gcccaggatc tgggagactt
     1861 ccagaacggc ttcaccggct tcgcgtcggc tctagcgccg acgctatcgt acaacgccac
     1921 ggccggcaac aattggtcgg atgcctatat aggcaaccta ccccacttcg gcattggggc
     1981 cgcggtcggc ttcacgtccg tcccgatagg cgagctgacc acgctcatcg ggaccatcgc
     2041 gagcggcgtc gagattccgg cggcgctctc ccagctcggc ctgcccgtgc cggccgccgc
     2101 gctgtcggct aagatcggcg gcttcatcct gcccttcgac ctcggcctca aggctatgat
     2161 gctgacgccc gagatgaccg ctagcctcga cgcgatgggc ttctcgttcg actacacgct
     2221 attcggcggc aacgtgcgct atgccctgct caagcag
//
LOCUS       DAOOLN010000217         2254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_59945_length_2254_cov_2.595725, whole genome shotgun sequence.
ACCESSION   DAOOLN010000217 DAOOLN010000000
VERSION     DAOOLN010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59945_length_2254_cov_2.595725"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="PLW80_03615"
     CDS             <1..1255
                     /locus_tag="PLW80_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPB65622.1"
                     /translation="LSTEHAKTADGGETVMGVDEKGPLAALVIKTQIDQFSGRLSYLK
                     VVRGVLASDTEYLNVQENKKEKAGKLYTAQGKKLEEVQELRAGDIGILAKSPSLKTNE
                     TISAPDAPVSFLPLRLPQPIHAIAISAVSKKEEDKLSELLHKASEEDKTFTIRFDSET
                     KETVIAGMGELHLNIILHKVKASQKIDVETRVPRVAYRETITKHSGGEYTHKKQTGGH
                     GQYARVVMEVEPLPRGEGYKFENKVFGGAVSKGFIPGIEKGIHQAMEFGTIAGYPVVD
                     VKSMLTDGKEHPVDSSEMAFKIAARGAFREACKAAGATLLEPIMRLAVFVDEKYLGDV
                     MSDLSGRRGRISGQEPIGGGIVEIKAEVPQAELLRYSIDLKAMTSGTGGFEVEFDHYS
                     PISGKIADDVAKAAAEFRIQEAEDE"
     gene            complement(1352..>2254)
                     /locus_tag="PLW80_03620"
     CDS             complement(1352..>2254)
                     /locus_tag="PLW80_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65623.1"
                     /translation="DAWAIKARHGLSIAELEERLWAELWAGAIGSDGFEAVRLAAGRG
                     FKRVATASPADPVGTDRRPTRGKPTRVPSALRARWKDGPVLVGAWFALALDDEGPADE
                     ADRLELDCRAVRAVVRRYGVLFPAMLDRELPRARWGNLARALRRMELSGELACGRFFE
                     GPDGPQFIGSDALRLFTDGFDDRLASMNACDAASPAGWPIGGLPEGLPSRLPVNRLST
                     AGGEPVCVARRSWRELELFREPDDPVVAHALAFALEARRRAVSPERRIAIATINGESA
                     ASSPYAGLLAGLGFEADRGVMTLW"
BASE COUNT          405 a          757 c          780 g          312 t
ORIGIN      
        1 gctgtcgacg gagcatgcca agacggccga cggcggcgag accgtcatgg gcgtcgacga
       61 gaagggaccc ttggccgcac tcgtgatcaa gacccagatc gaccagttct ccggaaggct
      121 atcgtacctg aaggtggtac gcggcgtgct agcctccgat accgagtacc tgaacgtgca
      181 ggagaacaag aaggagaagg cgggcaagct ctataccgcc cagggcaaga agctcgagga
      241 ggtccaggaa ctgcgcgccg gcgacatcgg catcctggcc aagagcccgt cgctcaagac
      301 gaacgagacc atctccgcgc cagacgcgcc ggtgtcgttc ctgccgctcc ggctgcccca
      361 gcctatccac gccatcgcga tcagcgccgt gtccaagaag gaagaggata agctgtcgga
      421 gctactccac aaggcttcgg aggaggataa gaccttcacg atacgcttcg actcggagac
      481 caaggagacg gtgatcgccg gcatgggcga gctgcacctg aatatcatcc tccataaggt
      541 caaggcctcg cagaagatcg acgtggagac ccgcgtgccg cgcgtagcgt accgcgagac
      601 gatcaccaag cactcgggcg gcgagtatac ccacaagaag cagaccggcg gacacggcca
      661 gtacgcccgc gtggtgatgg aggtggagcc cctgccgcgc ggcgagggct acaagttcga
      721 gaacaaggtc ttcggcggcg ccgtgtccaa gggtttcatc cccggcatcg agaagggcat
      781 ccaccaggcg atggagttcg gcacgatagc cggttacccg gtcgtcgacg ttaagagcat
      841 gttgacggac ggcaaggagc acccggtcga ctcgtccgag atggcgttca agatcgccgc
      901 gcgcggcgcg ttccgcgagg cctgcaaggc cgccggcgcc accctgctcg agccgatcat
      961 gaggctggcc gtcttcgtcg acgagaaata cctcggcgac gtgatgtccg acctgtcggg
     1021 ccgccgcggc cgcatctccg gccaggagcc gataggcggc ggcatcgtcg agatcaaggc
     1081 cgaggtgccg caggccgagc tcctgcgcta ttcgatcgac ctcaaggcca tgaccagcgg
     1141 caccggcggc ttcgaggtgg aattcgacca ttactcgccg atctccggca agatcgccga
     1201 tgacgtggcc aaggccgccg cggagttcag gatccaggaa gccgaggacg aatgattgcc
     1261 cggccgcgct agcgagccat ggggccgtcc tgcgggacgg tccctttttt tacgccccgc
     1321 ggatgtgtgc gtccgtcggc gccgttcagg gctaccagag cgtcatgacg ccgcggtcgg
     1381 cctcgaagcc gagcccggcg agcaggccgg cgtagggcga cgaggcggcg ctctcgccgt
     1441 tgatcgtggc gatggcgatg cggcgctccg gcgatactgc ccggcgcctg gcctcgagcg
     1501 cgaaggcgag agcgtgcgcg acgacggggt cgtccggctc gcggaacagt tccagctccc
     1561 gccacgatcg cctggccacg cagacgggct cgccgccggc cgtcgatagg cggttcaccg
     1621 gcaggcgcga cggtaagccc tcgggcaggc cgccgatggg ccaaccggcc gggctcgcgg
     1681 cgtcgcaggc gttcatcgac gcgaggcggt cgtcgaagcc gtcggtgaac agcctgagcg
     1741 cgtcggagcc gatgaactga gggccgtcgg ggccttcgaa gaaacggccg caggccagct
     1801 ccccggacag ctccatgcgc ctgagggccc tcgcgaggtt gccccagcga gccctcggca
     1861 gctcccggtc gagcatggcc gggaagagga cgccgtagcg cctgactacg gcccggaccg
     1921 cgcggcagtc cagctcgagg cggtcggcct cgtcggcggg gccttcatcg tcgagcgcga
     1981 gagcgaacca ggcgccgacc agaacggggc cgtccttcca gcgggcgcgc agcgccgacg
     2041 gcacgcgcgt tggctttccg cgcgtcggcc tacggtccgt tccgacggga tcggccgggg
     2101 acgccgtggc cacccgctta aagccgcgtc cggccgccag cctgacggcc tcgaaaccat
     2161 cagagcctat ggctccggcc cagagctccg cccacagccg ctcctccagc tccgctatcg
     2221 agaggccgtg cctggccttg atggcccagg cgtc
//
LOCUS       DAOOLN010000218         2253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_59983_length_2253_cov_2.089172, whole genome shotgun sequence.
ACCESSION   DAOOLN010000218 DAOOLN010000000
VERSION     DAOOLN010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59983_length_2253_cov_2.089172"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PLW80_03625"
     CDS             <1..638
                     /locus_tag="PLW80_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65624.1"
                     /translation="PYTVMGLENGVIHAFAVAAYNAAGEGSPSAIATTTPSNANAPHD
                     PSPADGADGVPLSGPLSLTWLTTNPNGNQLSFDVYLSLGSIPSSPIESGLPTPACSVD
                     GPFEPHAEYRWRVSVNNISQGYRSEGPEWSFMTANTPPPAPTLSDPAQGATDVDLSTW
                     FHWNHAHDPDDDAVAYDLLIDTANPPATVAATGLTANEYQVNGLTADTLYY"
     gene            795..1616
                     /locus_tag="PLW80_03630"
     CDS             795..1616
                     /locus_tag="PLW80_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65625.1"
                     /translation="MSGYRIYRSMATGGQGSTPIATTTGWSFEDTGFFIGDCAGTYYY
                     RVSAYNAAGEGPMSAESLATGSAPTIPVPSTELQPHNADYHFQDRYFLAITPNSDGAL
                     SSRQYRLNRIGPDPQLSWVSGDVAIYVNPIFAEAAGPVTCTYRLQSRNEWGDSAWSGE
                     FSVVVPAALPIIYPTITYAGAGRLDFTWSNPAGATKLRIFLVDQYYETAYSTSVAATP
                     PATSYSWTGLSSGSKYAFVIVPYDDTSTPKRIAARLFTGTDRWTVESSSLFVTVP"
     gene            1613..2155
                     /locus_tag="PLW80_03635"
     CDS             1613..2155
                     /locus_tag="PLW80_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65626.1"
                     /translation="MTTVSEWPQLRRLAAAVEIDWNGCLGSGASVDTDDHSLSFERDD
                     ETSTLDRSGVSARATPLRVDEGILAALGVAAPRWLTAKAGVAVDFELARQRRYGYDPP
                     SDPIRFFVESSETLSWRPVHFNELGLIVGPASLSTGFSSTIAWGAARAEATTAGSLVI
                     PRSARHSTNGEIPRPEPSSS"
BASE COUNT          409 a          783 c          693 g          368 t
ORIGIN      
        1 cgccgtatac ggtcatgggc ctcgagaacg gcgttatcca cgcgttcgcc gtggcggctt
       61 ataacgccgc cggagagggc tctcctagcg cgattgccac gacgacgccg tcgaacgcta
      121 acgcgccgca cgacccgtca ccggccgacg gagccgacgg cgttcccctt tcggggccgt
      181 tgtcgctcac ctggttgacg accaatccga acggcaacca actttccttc gacgtgtacc
      241 taagcctcgg ctcgattcca tcctcgccta tcgaatccgg gctgcccacg cccgcctgta
      301 gcgtcgacgg gccgttcgaa ccgcacgccg agtatcgctg gcgcgtctcg gtgaacaata
      361 taagccaggg ctaccgtagc gaggggcccg aatggagctt catgacggcg aataccccgc
      421 cgcccgcccc gacgctatcc gatccggccc agggcgcgac cgacgtagac ctatcgacct
      481 ggttccactg gaaccacgcc catgacccgg acgacgacgc cgtcgcctac gacctgctca
      541 tcgataccgc gaacccaccg gccacggtag ccgcgacggg ccttaccgcc aacgagtacc
      601 aggtgaacgg attaacggct gacacgctgt actactagcg cgtcgtggcc agggacggct
      661 acggcggatc ggccgagagt tcggtccgca gtttcaggac gatcgacccg ccggcggcgc
      721 cgaccgcgct cgcggcgagc gcagatcctc ggtacgccaa ggtaaaccta tcctggacga
      781 gccccggagg ccccgtgagc gggtatagga tctacagatc gatggctaca ggcggccagg
      841 gctcgacccc gatagccacg acaacgggtt ggagcttcga ggataccggc ttcttcatcg
      901 gggactgcgc cgggacgtac tactaccggg taagcgccta taacgccgcc ggagaggggc
      961 cgatgagcgc tgaaagcctc gcgaccggct cggcgcccac aataccggtt ccgtcgaccg
     1021 agttgcagcc tcataacgcc gactatcact tccaggaccg ctatttcctg gccatcactc
     1081 cgaattcgga tggagcgcta tcatccaggc agtaccgcct gaaccgaatc ggtcccgatc
     1141 cgcagctttc ctgggtttca ggcgacgtcg ccatctacgt caacccgatc ttcgccgagg
     1201 ctgccggacc cgttacatgc acctatcgcc tgcagagccg caacgaatgg ggcgattcgg
     1261 cgtggtcggg cgagttctcg gtcgtcgtgc cggccgcgct ccccattatc tacccgacga
     1321 tcacctacgc cggggcggga aggctcgact tcacgtggtc caatcccgcc ggagcgacga
     1381 agctcaggat tttcctcgtc gaccagtatt acgaaacggc ctactccacg agcgtcgcgg
     1441 cgacgccgcc tgcgacgagc tacagctgga cgggcctgag ttccggctcg aagtacgcgt
     1501 tcgtcatcgt gccgtatgac gatacctcga cgccgaagcg gatcgcggcc cggctattca
     1561 ccgggacgga ccgatggacg gtggaaagct cgtctttgtt cgtgaccgtg ccgtgacgac
     1621 cgtatccgaa tggccgcagc tacgcaggct cgccgccgcg gtcgagatcg actggaacgg
     1681 atgtctcggc tcgggcgcgt cggtcgacac cgacgaccac tcgctgtcct tcgagcgcga
     1741 cgacgagaca tcgaccctcg accgctcggg cgtcagcgcg cgggcaacgc ccctgcgagt
     1801 cgacgaggga atcctcgcgg ctctcggcgt ggccgcgccg cgatggctga ccgcgaaagc
     1861 cggcgtcgcc gtggacttcg agctggcgcg gcagcggcgc tacggctacg atccgccctc
     1921 cgaccctatc cggttcttcg tcgagtcgtc cgagacgctg tcatggcgtc cggtgcactt
     1981 caacgagctc ggcttgatcg tcggccctgc ctcgctgtcc accggcttca gctcgacgat
     2041 agcctggggc gccgcccgcg ccgaagcgac gacggccggc tcgctggtaa ttcccaggtc
     2101 cgctcgtcac tctacaaacg gggagatacc gaggccggag ccgagctcga gctaggatac
     2161 tgagccagcc cggtagcgct cgtagaagaa ggccgcggcg atcatcaggg cgtggtccca
     2221 aaagacgacc ccaacgcgaa ccaaaaactc gtc
//
LOCUS       DAOOLN010000219         2251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_60043_length_2251_cov_3.581512, whole genome shotgun sequence.
ACCESSION   DAOOLN010000219 DAOOLN010000000
VERSION     DAOOLN010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60043_length_2251_cov_3.581512"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PLW80_03640"
     CDS             complement(<1..356)
                     /locus_tag="PLW80_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65627.1"
                     /translation="MRTSCIVIVCAAIAMSCVSAPEPAREPPPASAQQGSSDGSTSQA
                     YKDRAVELVQGGSQGGSTGGAAGSSAAATTGSASGDAVDVPPPAPGVMTPEEEAFLNN
                     YLARLQYMVYYDEAAG"
     gene            complement(471..1919)
                     /locus_tag="PLW80_03645"
     CDS             complement(471..1919)
                     /locus_tag="PLW80_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65628.1"
                     /translation="MVDGKVLAVGYLSERADRLAWRAPMVNGVYEAKVELFPFRPIAG
                     STPPALAKAEIRLALSSAAPDPLADREAWSLFAFDGDLSDSGTRPRAVEPATIGSPYL
                     ETHPLGYGYLLGDGLGLSSESAILPVDAPNGSLAAFSAAFTLGKADDGLAEGTGALLS
                     AVLDSGQGLVIGVDTGYPYVAIGQALVRADSKLGSSLSRLAVDVSPAEGGAAIRFYID
                     DTPVGESLLRSELFSSVPGACSVAGPGGFIAVYDELRVLRGAYPAFRLSERAARGDAY
                     IAATGFEGSELEAGFAVEGAAKLEARRLTLGAGSSLSIFGSPPASKGSALRVDLEAGA
                     FISAVTLEGGGELAVSSAGSITLRGADTGLSVATRSSEAPFRVRPGVARYHISVAQSA
                     DGVKVFGSDGASVLVDARLDGASGWRLWPAVNTSATLAAVSVSAFDPAMVSSVRRDSA
                     AEGLSMLDSRAEATPTGRLSIAVETPSVLANL"
     gene            complement(1938..>2251)
                     /locus_tag="PLW80_03650"
     CDS             complement(1938..>2251)
                     /locus_tag="PLW80_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65629.1"
                     /translation="DADGDEAASIAFSPSPVASGNAVRVDDLFGELDPFSIPSGLPEG
                     YYELRARVLDSRGQALSTYSTYALVYSGSLGPVGLEVYPGGVERGKVSLLRLTGSLPS
                     G"
BASE COUNT          424 a          743 c          774 g          310 t
ORIGIN      
        1 atgccggcag cctcgtcgta gtagaccatg tactggagac gggccaggta gttgttcagg
       61 aaggcttcct cctcgggggt catcaccccg ggggccggag gcggcacgtc gacggcgtcg
      121 cccgaggcgg aacccgtggt cgcggcggcc gaggaaccgg cggcgccgcc agtcgatccg
      181 ccttgagagc cgccctgcac gagctcgacg gccctatcct tataagcctg cgacgtactc
      241 ccgtcggacg agccttgctg ggccgacgcg ggcgggggct ctcgagccgg ctccggagcg
      301 ctgacgcatg acatggctat ggcggcgcag acgatgacga tacacgatgt tctcatacgg
      361 cccttccaca aatacggtaa ggttactata gcgcgctcgc cgcgctaaaa cagcacggta
      421 ccaatggtag tcaattacta gaaatattca aggctcgggg aagaaaaggg ttacagattc
      481 gccaggaccg acggcgtctc gacggcgatg gatagcctgc cagtcggagt agcctcggcg
      541 cgagagtcga gcatggatag gccctcggcc gcagagtcgc ggcggacgga cgataccatg
      601 gcagggtcga aggccgagac cgacacggcg gccagggtcg ccgacgtatt cacagcaggc
      661 cacaggcgcc atccagaagc gccgtcgaga cgagcgtcca cgagtaccga ggcgccgtcg
      721 gagccgaaga ccttgacgcc gtccgcggac tgggcgaccg atatatggta gcgcgccacg
      781 ccgggcctga cacggaacgg cgcttccgag gacctggtcg ccacggacaa gccggtatcc
      841 gcgccacgta gcgttatgct ccctgctgac gaaacggcca gctccccacc gccctcgagc
      901 gtcacggcgg atatgaacgc gcccgcctcg aggtcgacgc gcagcgctga gcctttcgag
      961 gccgggggcg agccgaaaat cgacaggctc gagccggcgc ccagcgttag ccggcgggcc
     1021 tccagcttcg cggccccttc gacggcgaag cccgcctcga gctcggagcc ctcgaaaccg
     1081 gtcgccgcga tgtacgcgtc gccgcgggcg gcccgctccg acaggcggaa cgccgggtag
     1141 gcgccgcgca gcacgcgtag ctcatcgtat acggctatga acccgcccgg cccggccacg
     1201 gagcaggcgc cgggaacgga tgagaacagc tcgcttcgaa gcaggctttc gccaacgggc
     1261 gtatcgtcga tgtagaagcg gatagcggcg ccgccctcgg caggcgatac gtctaccgcg
     1321 agccgactca ggctcgagcc tagcttcgag tcggcccgta ccaacgcctg accgatcgcg
     1381 acgtatggat aaccggtatc gacgccgatg accaagccct ggcccgagtc caggactgcg
     1441 gatagcaagg cgccggtacc ctcggccagg ccatcatcgg ccttgccgag cgtgaacgcg
     1501 gcggagaacg ccgcgaggct tccgttaggc gcgtccacgg gcagtatcgc cgactcggac
     1561 gacaggccga gcccgtcgcc gagcaggtac ccatatccga gcggatgggt ctcgagatac
     1621 ggtgaaccga tagttgccgg ctcgaccgcc cgtggcctcg tgccagaatc ggacaggtcg
     1681 ccgtcgaagg cgaatagcga ccacgcctcg cgatcggcga gtggatcggg cgcggccgag
     1741 gagagcgcga gcctgatctc ggctttggcg agtgcgggag gcgtcgaccc ggcgatgggc
     1801 ctgaagggga agagctcgac cttcgcctcg tagacgccgt tgaccatagg cgcacgccaa
     1861 gccagcctgt cggcccgttc ggataggtag ccgaccgcta gtactttccc gtcgaccacc
     1921 catcgaatcc aggggtccta gccggacggg agcgagccgg tcaggcgtag cagcgacacc
     1981 ttgccgcgct cgacgccgcc tggatacacc tcgagcccga ccggcccgag cgatccggaa
     2041 tagacgaggg cgtaggtaga ataggtcgac agcgcctggc cgcgcgagtc gagtacccgg
     2101 gcgcgtagct cgtagtaccc ctcgggcagg cccgacggaa tggaaaacgg gtcgagttcg
     2161 ccgaacaggt cgtcgacccg taccgcgttg cccgaagcga ccggagacgg ggagaacgcg
     2221 atggaggccg cctcgtcgcc gtcggcgtcg t
//
LOCUS       DAOOLN010000220         2241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_60439_length_2241_cov_3.276304, whole genome shotgun sequence.
ACCESSION   DAOOLN010000220 DAOOLN010000000
VERSION     DAOOLN010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60439_length_2241_cov_3.276304"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1007)
                     /locus_tag="PLW80_03655"
     CDS             complement(165..1007)
                     /locus_tag="PLW80_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HPB65630.1"
                     /translation="MLAESQTAVGISDRQFKRLSAFIEKELGIKMPDTKRVMLESRLQ
                     KRLRALRIDTYDEYIDRVFSGDDAELIHMIDVVTTNKTDFFREPDHFEILTKRLLPKA
                     LERDGERGTMSFWSAGCATGEEPYTLAMVLEEFAVAHRGFDYRIIASDISTSALDAAI
                     HAVYDANRVIPVPPSYKKKYLLRSRDPAKNEVRIKRELRDKVGFLRINFMDDSFPFDG
                     QFDVIFCRNVIIYFDRATQERILGRMCRYLRTGGNLILGHSETLTSMDLPLRGLAPTV
                     YERL"
     gene            complement(1115..>2241)
                     /locus_tag="PLW80_03660"
     CDS             complement(1115..>2241)
                     /locus_tag="PLW80_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65631.1"
                     /translation="RAISELLSGDYRPTVSALESVYSLLASDESRSAFGREFAGYTGA
                     VYRDDDRDGYPESVTEYRDGLPSSWSLDADQDGEPEFAASFDSGAPARLSARSGSTTV
                     VVAYAPWPYAENVSYADTSGSRMYSFGPSSMPLPLLSLSRIAGLAGAPYAVSRSGYGL
                     PTEAAAKRAAYAMIESGPRGASTTVELSDGQASRSWWVDEWRRVGRTVYRNGVPDDER
                     MDVDADGRFESRRVWARGPDGVPAPAYAEADLDGDGLYEYRETLSGPSVQSWDYDGDG
                     AIDLSLERLPGGRSLYRVFARDGGLTEAMLEGGRLSSVVERGAPVPLVDDSGGLVRWI
                     GAKPFDFGGRVPPTGRGSRDGVMYAVHDIGGRLYAQVVRE"
BASE COUNT          393 a          730 c          724 g          394 t
ORIGIN      
        1 gctcggtttc aagcttcttg agcgcgaaga tgggatcgga ggctacggcc aggacctcta
       61 tgcccgggtc agaatccagg agttccttga gcgtctgcct gactacggcg gagtcgtcta
      121 cgaccatgac cttgatgcgt tccatctggc tcccctcggg tcaactataa gcgctcgtag
      181 acggtgggcg caagcccgcg cagcgggagg tccatgctcg tcagcgtctc ggagtgtcca
      241 aggatgaggt tgccgccggt ccgcaggtac cggcacatgc gcccgagtat gcgctcctgc
      301 gtcgctctgt cgaagtagat gatcacgttc ctacagaaga tcacgtcgaa ctgcccgtcg
      361 aacgggaagc tgtcatccat gaagttgatg cgcaggaagc cgaccttgtc gcgtagctct
      421 cgcttgatgc gcacctcgtt cttcgcggga tcgcggctcc tgagcaggta tttcttcttg
      481 tacgacggcg gcacagggat taccctattg gcgtcgtaga cggcgtgtat cgccgcgtcg
      541 agggcgctcg tcgaaatgtc cgaggcgatg atgcggtagt cgaagcccct atgggctacg
      601 gcgaactcct cgaggaccat ggcgagggta tagggctctt cgccggtggc gcagcccgct
      661 gaccagaacg acatcgtgcc gcgttcgccg tcgcgctcga gcgccttggg taggagccgc
      721 ttagtcagta tctcgaaatg gtccggctcc ctgaagaaat cggtcttatt ggtggtgacg
      781 acgtctatca tgtggatcag ctcggcgtcg tccccggaga agacgcggtc gatgtactcg
      841 tcgtaggtat cgatccgcaa cgcgcgcagc cgcttctgaa ggcggctctc gagcattacc
      901 cgcttggtgt cgggcatctt gatgccgagt tccttctcta tgaaggccga caagcgcttg
      961 aactgcctgt cggagatacc gacggcggtt tgactctcag cgagcatggg caacggcatc
     1021 ctccatacgc gtaccgtggc gttcgtcgat atcagtgtat tggctacgcg cagggtccgc
     1081 aagcaggaat cgccgggacc ctcgcgtgct ccgcctattc ccgtacgacc tgggcgtaca
     1141 accggccccc gatatcgtgc acggcgtaca tgacgccatc tcgcgaaccg cgcccggttg
     1201 gcgggacccg gccgccaaaa tcgaagggct tcgcgcctat ccagcgcacc agcccgccgg
     1261 agtcgtcgac gagtggtacc ggcgctccgc gctcgaccac gctcgacagc cgccctccct
     1321 cgagcatcgc ctcggtcaag ccgccgtcgc gcgcgaatac gcgatacagg ctacggccgc
     1381 ccggcaggcg ttccaggctc aggtcgatgg cgccgtcgcc atcgtagtcc caagactgta
     1441 cggacgggcc ggacagcgtc tcacgatact cgtagagccc gtcgccgtcc aggtcggcct
     1501 cggcgtaggc cggcgccggc acgccgtcag ggccgcgggc ccagacccgg cgcgactcga
     1561 aacgcccgtc ggcgtcgacg tccatacgct catcgtcagg cacgccgttc cgatagacgg
     1621 tcctgccgac gcggcgccac tcgtccaccc accacgagcg cgaggcctgg ccgtcgctca
     1681 gttccaccgt ggtagaagcg ccgcgaggtc cggattcgat catagcgtac gcggcccgtt
     1741 tggcggcggc ctcggtcggt agcccgtagc cgctccgcga tacggcgtac ggcgcccccg
     1801 cgagcccggc gatgcggctc agcgacagca gcgggagcgg catcgatgag gggccgaagg
     1861 aatacatcct cgaaccagag gtgtccgcgt acgatacgtt ctcagcatag ggccacggcg
     1921 catacgcgac gacgacggtc gtcgagcccg atcgcgccga cagcctggct ggagcgccgg
     1981 agtcgaaaga cgcggcaaat tcaggctcgc catcctggtc ggcgtcgagg gaccacgacg
     2041 aaggtaggcc gtcacggtac tcggtgaccg attcaggata gccatcgcgg tcgtcgtcgc
     2101 ggtacacggc tcccgtatac ccggcgaact cgcgaccgaa cgcggatcgg gactcgtcgc
     2161 tggccagcag ggaatagacg gactcgagcg ccgataccgt cgggcgatag tcgccggaca
     2221 ggagctcgga tatagcccgc c
//
LOCUS       DAOOLN010000221         2241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_60446_length_2241_cov_2.852699, whole genome shotgun sequence.
ACCESSION   DAOOLN010000221 DAOOLN010000000
VERSION     DAOOLN010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60446_length_2241_cov_2.852699"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..766
                     /locus_tag="PLW80_03665"
     CDS             131..766
                     /locus_tag="PLW80_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB65632.1"
                     /translation="MAIRYHTATFENIPSEKRERILRAASRVLGRDGIGGARMADVAA
                     EAGVSHGSMFSYFPTKDDMIRAVIARGMSLQAERFAASDDEGAGFAATVRTVFRSAWD
                     EASAEPELISLWLSLSLVENARFADAILPLERDSTERWRRVVARGKASGEIDQGIDDG
                     AAAYLLDALAAQLMKSRASGLERAKLELFVGTGLAEAAPDRMTDAVVAMLR"
     gene            842..>2241
                     /locus_tag="PLW80_03670"
     CDS             842..>2241
                     /locus_tag="PLW80_03670"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HPB65633.1"
                     /translation="MDRFSDGFLFGASSSAFQIEGSLDRDGRGPSVWDAFIARPGRER
                     LRSDAATACDHYARWADDVAIAGELGLDAYRFSVSWPRIQPDGRGEPNRAGLDFYSRL
                     VDGLLERDVRPFPTLFHWDLPLALQRDSGGFRDRDTAARFEDYAGIVARALGDRVKDW
                     ITINEPFEYACFGHLFGTHAPGVKSPLAYLQVVHHQLLAHGRAVSAIRSEAAGARVGI
                     ALSWTPVHPLRDTEADEKAAARANAFMNGVSFDPILLGRYPALVTRGLLRPPVRDGDF
                     ETISTPLDFVGLNYYSREKARNAPLVPFIRADVTGKEPRELPESDDRTAMGWEVYHRG
                     LVEILAELRGRYGNPPVIVTEFGSAWSDSVETDAATGRARVGDTRRVRYLRDALAAMR
                     SAIEAGSDLRGAFVWSLTDNFEWAEGNAKRFGLVHVDYGTQARTVKDSGRWYAGLIAS
                     RVLDASSRVLDASSRVL"
BASE COUNT          352 a          782 c          735 g          372 t
ORIGIN      
        1 atccgttcca tcgccacctc tctgattggt tagcgactat agcaccaagt ccttgcgcgt
       61 atccaccaat aaggttcgta acactgaccg tgccttgacc gctcaggccg cttgcgacga
      121 tactcgcccc atggcaatcc ggtatcatac cgccaccttc gagaacattc cctcggagaa
      181 gcgcgagcgc atcctccggg ccgcctcgcg cgtgctcggc cgcgacggca tcggcggcgc
      241 gagaatggcg gacgtcgccg ccgaggccgg cgtctcccat ggctccatgt tcagctattt
      301 cccgaccaag gacgatatga tccgcgcggt catcgcccgg ggcatgagcc tgcaggccga
      361 gcgcttcgcg gcgtcggacg acgaaggggc aggcttcgcc gcgacggtcc gcaccgtctt
      421 caggagcgcc tgggacgagg cgagcgccga gcccgaactc atatcgctct ggctgtccct
      481 atcgctcgtc gagaacgccc gcttcgccga cgccatcctg ccgctcgagc gcgactcgac
      541 cgagcgatgg cgccgcgtcg tcgcccgtgg caaggcttcc ggcgagatcg accaggggat
      601 cgacgacggc gcggccgcct accttctcga cgcgctggcc gcccagctga tgaagagccg
      661 cgccagcggg ctcgaacgcg ccaagctcga gctgttcgtc ggcacggggc tggccgaggc
      721 cgctcccgac cgtatgaccg acgccgtcgt cgccatgcta cgatagcctc attctgatga
      781 ttgactggtc agtcaacgca tggtagccta gcgtcagctt gatgacgagg agtatcggcg
      841 tatggatcgg ttcagtgacg gctttttgtt cggggcttca tcgtcggcgt tccagatcga
      901 gggaagcctc gaccgcgacg gccgcggccc gtccgtctgg gacgccttca tagcgaggcc
      961 gggccgggag cgcctgcgtt ccgatgccgc gacagcctgc gatcattacg ccagatgggc
     1021 cgacgatgtc gccatagccg gcgagctcgg gctcgacgcg tatcgcttct ccgtatcgtg
     1081 gccgcggatc cagccggacg gccgcggtga accgaatcgg gcgggcctgg acttctactc
     1141 gcgcctcgtc gacggcctcc tggagcggga cgtgcggcct ttcccgacgc tgttccactg
     1201 ggatctgccc ttggcgctcc agcgcgactc gggcggcttc agggaccgcg atacggccgc
     1261 ccgcttcgag gactacgccg gtatcgtcgc tcgcgcgctc ggcgaccggg tcaaggattg
     1321 gatcaccatc aacgagccct tcgagtacgc gtgttttggc cacctcttcg gtacccacgc
     1381 accgggcgtc aagagtcccc tggcctacct tcaggtcgtc catcatcagc tactggccca
     1441 tggccgggcc gtctccgcca tacgctcgga ggccgccggc gcccgggtcg gcatagccct
     1501 cagctggacg ccggtgcatc ccctacgcga taccgaggcg gacgagaagg ccgccgcccg
     1561 agccaacgcg ttcatgaacg gcgtcagctt cgacccaatc ctgctcgggc gctatcccgc
     1621 cctcgtgacg agggggctac tcaggccgcc tgtcagggac ggcgacttcg aaaccatctc
     1681 gacgccgctc gatttcgtcg gcctgaacta ctacagtcgg gaaaaggcgc gcaacgctcc
     1741 gctcgtgccg ttcatccggg cggacgtgac cggcaaggag ccgcgcgagc tgcccgagag
     1801 cgacgatagg accgcgatgg gctgggaggt ctaccaccgg gggctcgtcg agatcctggc
     1861 cgagctgcgc ggccgctacg ggaacccgcc ggtcatcgtc accgagttcg gctcggcctg
     1921 gagcgactcg gtcgagacgg acgcggctac gggccgggcc agggtcggcg acacgaggcg
     1981 cgtccgctac ctgcgcgacg cgctcgccgc gatgcgctcg gccatcgagg ccggctcgga
     2041 cctgcgcggc gccttcgtct ggagcctgac cgacaacttc gagtgggccg agggcaacgc
     2101 caagcgcttc ggcctggtac acgtcgacta cggcacgcag gcccgtaccg tgaaggactc
     2161 gggccgctgg tacgccgggc tcatcgcgtc gagagtactc gacgcgtcgt cgagagtact
     2221 cgacgcgtcg tcgagagtac t
//
LOCUS       DAOOLN010000222         2236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_60632_length_2236_cov_2.437873, whole genome shotgun sequence.
ACCESSION   DAOOLN010000222 DAOOLN010000000
VERSION     DAOOLN010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60632_length_2236_cov_2.437873"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLW80_03675"
     CDS             complement(<1..328)
                     /locus_tag="PLW80_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HPB65634.1"
                     /translation="MKESILYYDCFSGISGDMNLSALVGLGVPEDHLRAELEKLGLDG
                     WSLRLTRDSKHGIFGLRADVELAGHEHNHEHAHEHNYEHGHEHAHKHGHEHAHEHNHE
                     HEHDHEH"
     gene            complement(325..1146)
                     /gene="larE"
                     /locus_tag="PLW80_03680"
     CDS             complement(325..1146)
                     /gene="larE"
                     /locus_tag="PLW80_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HPB65635.1"
                     /translation="MDSIAKEQALVASLKKYGSLAIAFSGGVDSSYLCAIAVEALGSR
                     ALAITVDSPLLPRHELEDARRVAAFVGIEHVIMEEGDIDETVAANPPDRCYLCKRIKM
                     SAMLRVAEDRGLAALAEGSNIDDLSDYRPGRLANAELGIRSPLSDAGMTKDDIRELSR
                     RRGLATWDKPAFACLASRVPYGDRLSAPLLARIEAAEDYLRDLGFRQCRVRSHDGTAR
                     IEVAPDERVKFFDETMMDDVSGRLKRLGYDFVSLELEGYRMGSMNRALDTERTEL"
     gene            complement(1198..1959)
                     /locus_tag="PLW80_03685"
     CDS             complement(1198..1959)
                     /locus_tag="PLW80_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB65636.1"
                     /translation="MNACGLDLNAIRFSYPDGRPVLGGLSLSLKPGSKLALVGANGSG
                     KSTLLGLVAGLLQPQSGSIDIGGLTLCKRNLKEARQRIGFVFQDPDDQLFTGSVYDDV
                     AFGPRNGGLDQAAVRERADTALSTVGIAGLADRPPFRLSGGEKRMAAIATVLSMDPGL
                     LVLDEPSAALDPKARRRLISLLSSLSQSMLIATHDLELALELCDETAILFGGAVEAAG
                     RSKDLLADEGLLDRCGLEAPLSVRACPVCGKRAAR"
     gene            complement(1956..>2236)
                     /locus_tag="PLW80_03690"
     CDS             complement(1956..>2236)
                     /locus_tag="PLW80_03690"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HPB65637.1"
                     /translation="LRSGGRRGVDRSVLGPMIGGLLLRSYDRAGRVYDAMSLRGFDGE
                     YRVGQASRFGVPDALYCLAWLGYFAIVRLVDLAEALGRIANRLAGGML"
BASE COUNT          378 a          705 c          743 g          410 t
ORIGIN      
        1 cgtgctcgtg atcatgctcg tgctcgtggt tgtgctcatg ggcgtgctca tggccatgct
       61 tatgggcgtg ctcatggcca tgctcatagt tgtgttcatg ggcgtgctca tggttgtgct
      121 catggccagc cagctcgacg tcggcgcgaa gcccgaagat gccgtgcttc gagtcgcggg
      181 taaggcgtag cgaccaaccg tccaaaccga gtttctcgag ctcggcccgg aggtgatcct
      241 ccgggacgcc gaggccgacg agggccgaga ggttcatgtc gccggagatg cccgagaaac
      301 agtcataata taggatgctt tccttcataa ttccgtcctt tcggtatcga gcgccctgtt
      361 catgcttccc atacgatagc cctcgagctc gaggctgacg aagtcgtagc cgagtcgctt
      421 gagccggccc gatacgtcgt ccatcatcgt ttcgtcgaag aatttgaccc gttcgtccgg
      481 ggccacctct atgcgggcgg tgccgtcatg gctcctgacc ctgcattgcc tgaagccgag
      541 gtcgcgcaga tagtcctcgg ccgcctcgat gcgcgccagg aggggagcgc ttagcctgtc
      601 accgtagggc acgcgcgagg ccaggcatgc gaaggccggc ttatcccagg tggcgaggcc
      661 gcggcgccgg gatagctcgc gtatatcgtc cttggtcatg ccggcgtcgg agagcggcga
      721 gcggataccg agctcagcgt tcgcgaggcg cccgggacgg tagtcggaga gatcgtcgat
      781 attggaacct tccgcgaggg ccgcgaggcc cctgtcttcc gcgactctca gcatggcgct
      841 catcttgatg cgcttacaga ggtagcagcg atcgggaggg ttggcggcga cggtctcgtc
      901 gatgtcgcct tcctccatga tcacatgctc gataccgacg aaggcggcta cccggcgcgc
      961 gtcctccaac tcgtgacgtg gtaggagcgg cgagtcgacg gtgatggcca gggccctgga
     1021 accgagcgcc tcgaccgcta tggcgcagag gtagctggaa tcgacgcctc cggagaacgc
     1081 gatcgcgagg gatccgtact tcttgagcga ggcgactagg gcctgttctt tcgcgatact
     1141 atccatctcg tagtccttct tgatgtgcgt ggcgggtcga gtataccacg ctgagcgtta
     1201 gcgcgccgcc cgctttccgc agacggggca ggcgcggacg gatagcggcg cctcgagccc
     1261 gcagcgatcg aggagacctt cgtcggctag taggtccttt gatcttccgg cggcctcgac
     1321 ggcgcctccg aacaggatgg ccgtctcatc gcatagctcc agggccagct cgaggtcgtg
     1381 ggtcgctatg agcatgctct gagacaggct cgacaggagg ctaatgagcc ggcgcctggc
     1441 cttggggtcg agcgccgcgc tcggttcgtc cagaacgagg aggcccggat ccatcgacag
     1501 caccgtcgct atggccgcca tgcgcttctc gccgcccgag agcctgaacg gaggccggtc
     1561 ggccaggccg gctatgccaa ccgtgctcaa ggccgtatcc gcccgttccc ggactgcggc
     1621 ttggtcaagt cccccgttgc gcgggccgaa ggctacatcg tcgtagacgc taccggtgaa
     1681 cagctggtcg tccgggtcct gaaagacgaa gccgatcctc tggcgcgctt ccttaaggtt
     1741 gcgcttgcat agcgtgaggc ccccaatgtc gatgctaccc gactggggct ggagcagtcc
     1801 cgccacgagg ccgagcagcg tcgacttgcc cgagccgttg gcgcctacca gcgccagctt
     1861 ggagccaggt ttaaggctca ggctcaggcc tccgagcaca ggcctcccgt ccggatagga
     1921 gaagcgaatc gcgttcagat cgagtccgca ggcgttcaaa gcatccctcc cgccaggcgg
     1981 ttagcgatcc gcccgagcgc ctctgcaagg tcgactagtc gcacgatggc gaaatagccc
     2041 agccaggcca ggcagtagag ggcgtccggc acgccgaagc gcgaggcctg gccgacgcgg
     2101 tactcgccgt cgaagccgcg taggctcatg gcgtcgtata cgcgcccggc gcggtcgtat
     2161 gaacggagga ggaggccccc gatcatcgga cccaagacgg aacgatcgac cccgcgccgt
     2221 ccgcccgagc gtagtt
//
LOCUS       DAOOLN010000223         2228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_60945_length_2228_cov_2.605154, whole genome shotgun sequence.
ACCESSION   DAOOLN010000223 DAOOLN010000000
VERSION     DAOOLN010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60945_length_2228_cov_2.605154"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /locus_tag="PLW80_03695"
     CDS             complement(<1..1619)
                     /locus_tag="PLW80_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPB65638.1"
                     /translation="MLRTIRARLMAINLLLALLIGSLFAANYLSLAGLGALQRDGRTR
                     SMDAKAVAEASYVGSDLFAVIATLIINRDLAEFDAAWPAAKARASERMDAALAVAAGD
                     EDRTLANRARGALDELIAAVEGALAPLAAGTDADRTRISPSMRVLGYQIRLLVDQVER
                     DLKAVSESVEREAVLADEAFAALSARTAATGLALGLVAIALAVGTMMANLRLVLRPLR
                     SQLGLLDDMAGAEADLTRRLDQARDDEYGRLGRSFNAFMERLVASVDAVKSAAAALAG
                     QTAALAERMEDTAAAVEQIGANVESIRKRSEAQALAAGRSAESLERVTTGLTELDALI
                     ASQAASVAESSAAIEQMVANMRSVAGAVESNSASIEELQRSAIDGKEWMDEVASLLRG
                     MLSDSAVLSEAGAVIQGISSQTNLLAMNAAIEAAHAGEAGRGFSVVAEEIQKLSELSS
                     DEGKRIVDVLERLKRAMESLEATASRSQSGFARVLEQTTLVASHDAVIKGAMDEQRSG
                     GSQVLEAIHRIGDITAEVRSGSECMLGASAEA"
     gene            1797..>2228
                     /locus_tag="PLW80_03700"
     CDS             1797..>2228
                     /locus_tag="PLW80_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65639.1"
                     /translation="MRILSVTLMASSLILVPTLQAQEADGGAEYSIETNLAYIALGAV
                     SCTGGNVFIVAPLYSQLRVFGRFSVDLASAYIYNSHPTLATPSSMILAEAGASYHPSG
                     AIRGWTFGLLPGVTYSFDSRIAGFSISANCGKAVITPLPVVC"
BASE COUNT          323 a          794 c          713 g          398 t
ORIGIN      
        1 gcctcggcgc tggctcctag catgcattcc gaaccggaac gcacctctgc cgtgatgtcg
       61 ccgatacgat ggatggcctc gagcacctgg ctgccgccgg agcgctgctc gtccatggcg
      121 cccttgatca cggcgtcgtg ggaagccacg agcgtcgtct gctccaatac gcgggcgaag
      181 cccgactggg atctgctcgc ggtggcctcg agggactcca tggcccgctt gagccgttcc
      241 aggacgtcga cgatgcgctt gccctcgtcg ctcgacagtt ccgacagctt ctggatctct
      301 tccgctacca ccgagaatcc gcgccccgcc tcgccggcgt gggccgcctc tatggccgca
      361 ttcatcgcca gcaggttggt ctggctcgag atgccctgga ttacggcgcc cgcttcggac
      421 aagacggccg agtcgctcag catgccgcgc aggagcgaag ccacctcgtc catccattcc
      481 ttgccgtcga tggccgaccg ttggagctct tcgatggacg cggagttcga ctcgaccgcg
      541 ccggctaccg accgcatatt ggccaccatc tgctcgatag cggccgacga ctcggcgacg
      601 ctggccgctt ggctcgcgat gagcgcgtcc aattcggtca ggccggtcgt gacccgttct
      661 agcgactcgg cgctccgccc cgcggcgagg gcctgggctt ccgaccgttt acggatgctc
      721 tccacgttgg cgccgatctg ctcgaccgcc gccgcggtat cctccatgcg ctcggccagg
      781 gcggccgtct ggccggccag cgccgccgcg gcggacttga ccgcgtcgac cgaagcgacc
      841 aggcgctcca tgaacgcgtt gaaggaacgg ccgagcctac cgtactcgtc gtccctggcc
      901 tgatccagcc ttcgcgtcag gtcggcctcg gccccggcca tgtcatcgag gaggcccagc
      961 tgcgagcgga gcgggcgcag cacgagccgt aggttcgcca tcatggtccc gacggccagc
     1021 gctatggcga ccagcccgag cgccaagccg gtcgccgccg tcctggctga cagggcggcg
     1081 aaggcttcgt ccgcgagcac ggcttctcgt tccaccgact cgctgaccgc cttcaggtcc
     1141 cgttcgacct ggtcgacgag cagccgtatc tggtagccga gcacgcgcat cgacgggctt
     1201 atcctggtgc ggtccgcgtc ggtcccggcg gccagcggcg ccagcgctcc ctccaccgcg
     1261 gcgatcagct cgtccagcgc gccgcgggcc cgattcgcta gcgtccggtc ttcgtcgccg
     1321 gccgcgacgg cgagggcggc gtccatccgc tcggaagccc tcgccttggc cgccggccat
     1381 gcggcgtcga attccgccag gtccctgttt atgataagcg tggcgatgac ggcgaacagg
     1441 tcgctcccta cgtacgaggc ctccgccacc gccttggcgt ccatcgagcg cgtcctgccg
     1501 tcccgctgaa gcgctccgag gccggccaga gacaggtagt tggcggcgaa tagcgagccg
     1561 ataagcagcg cgagcagcag gttgatcgcc atgagcctgg ccctgatcgt acgtaacacc
     1621 cgtatcacct ccaaagcgcg gaacggccag tatgtaccgg aatctgcgta ccgtcaaact
     1681 gtgcggggtg tattagctcg cgtccggcta tactgtacgt gacaaccccg ctttggcgcg
     1741 tcgtcggtgc gcgcaacgga cagcgggctc cggatactag ctctaggagg tatacgatgc
     1801 gtattctctc cgtaacgctg atggcctcgt ccttgatcct cgtcccgacg cttcaggccc
     1861 aggaggctga cggcggcgcc gagtactcga tcgagacgaa cctggcgtat atagcgctcg
     1921 gggcggtctc gtgcacggga ggcaacgtct tcatcgtcgc gcccctgtac tcccagttgc
     1981 gggtcttcgg gcgcttcagc gtcgatctgg cctccgccta tatctacaac agccacccga
     2041 ccctcgcgac gccgagctcg atgatcctcg ccgaggccgg cgcgtcgtac cacccgtccg
     2101 gggcgatccg cgggtggacc ttcgggctgc tgccgggcgt cacctactcg ttcgactcgc
     2161 gtatcgccgg gttctcgatc tcggcgaact gcgggaaggc ggtgatcacg cccttgccgg
     2221 tcgtgtgc
//
LOCUS       DAOOLN010000224         2211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_61626_length_2211_cov_3.154453, whole genome shotgun sequence.
ACCESSION   DAOOLN010000224 DAOOLN010000000
VERSION     DAOOLN010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61626_length_2211_cov_3.154453"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PLW80_03705"
     CDS             complement(<1..690)
                     /locus_tag="PLW80_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65640.1"
                     /translation="MRRPSARGLACIVAFSAMLAPAARVSAQASSGTDVSSSALTIGW
                     AELAVSDGVGDEYRNAAVLVPRQLQRALAFADLRFPSDVEAAAASTRAALAAEELARK
                     AVADARAKRDLAALAVRDPAKRGRDVATAAAAVKKAEAALEAILATNRETNRVRSSAD
                     RAGPGSTTAEGRSLALALSSANSGETLLPSTLDPASLCKDKKLDLLVYGSLRVRGGFL
                     AIDLSLYVASLG"
     gene            complement(698..1835)
                     /gene="prfB"
                     /locus_tag="PLW80_03710"
     CDS             complement(join(698..1765,1767..1835))
                     /gene="prfB"
                     /locus_tag="PLW80_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704758.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPB65641.1"
                     /translation="MLEDYMAPIAQLKKEVLEIWGRLDAATIRSKIETKESQSSEPGF
                     WNDQKRSERIMGELKVLRNRLETWEEIKRSTDALDEIYAMAIEEGEGSLEGEIASSLA
                     GVRDRFDKAVTLELMTGEADRTGVFLSIHAGSGGTEACDWAGMLMRMYTRWAERRGFK
                     YEIVDMLEAEGGIKSVTMEIDGAYAYGHLRGESGVHRLVRISPFDANARRHTSFASVS
                     AFPVIDDSIDVTVKPDEIRIDTYRAGGAGGQKVNKTDSAVRITHLATGIVVTCQNERS
                     QHKNKDVAMQVLKSRLYDLYRKEKDKENEKYVGDKKEIAWGSQIRSYVFQPYTMVKDH
                     RTKFFVGNIHGVMDGDLDPFIDSYLHFLWKGGTVQADGDDDDEV"
     gene            complement(1852..2142)
                     /locus_tag="PLW80_03715"
     CDS             complement(1852..2142)
                     /locus_tag="PLW80_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas2"
                     /protein_id="HPB65642.1"
                     /translation="MFVSVVCDLGGEDSWAAVYALLPQYGFEKVQRACFESVRATEKQ
                     LVALKRDIDKVTDYYDTVRIYQYPVEGRLAISILKENRWKRVVVRAPAEKAP"
BASE COUNT          339 a          721 c          745 g          406 t
ORIGIN      
        1 gccgagtgac gccacgtaga gcgacaggtc gatggccagg aagccgccgc ggacgcgtag
       61 tgagccgtag acgagcaaat cgagcttctt atccttgcag agcgaggccg ggtcgagcgt
      121 cgaggggagg agcgtctcgc cggagttggc cgacgagagg gcgagagcca ggctacggcc
      181 ctcggcggtc gtactgcccg gcccggctct atcggccgag gagcgcacgc ggttcgtctc
      241 gcggttcgtt gcaaggatgg cctcgagggc ggcctcggcc ttcttgactg cggcggccgc
      301 cgttgccaca tcgcgaccgc gcttggcggg atcgcggacg gccagggcgg cgagatcgcg
      361 cttggccctc gcgtcggcca cggccttcct ggccagttcc tcggcggcca gggcggcccg
      421 cgtcgaggca gccgcggcct cgacgtcgct cgggaagcgc aggtcagcga aggccagggc
      481 gcgctggagc tgacggggca cgagcaccgc ggcgttgcgg tactcgtcgc cgacaccgtc
      541 ggagacggcg agctcggccc agcctatcgt caacgcggag gacgatacgt cagtacccga
      601 cgaagcctgg gccgacaccc gcgccgccgg cgcgagcatc gccgagaacg ctacgataca
      661 ggctaacccg cgggccgacg gccttctcat cggagcccta cacctcgtcg tcgtcgtcgc
      721 cgtcagcctg gacggtgccg cccttccata ggaagtggag gtacgagtcg atgaacgggt
      781 cgaggtcgcc gtccatgacg ccgtggatgt tgccgacgaa aaacttggtc cggtgatcct
      841 tgaccatcgt atagggctgg aacacgtagg agcgtatctg ggagccccag gctatctcct
      901 tcttgtcgcc gacgtacttc tcgttctcct tgtctttctc cttgcggtac aggtcgtaga
      961 gccggctctt gagcacctgc atcgccacgt ccttgttctt gtgctggctg cgctcgttct
     1021 ggcaggtgac gacgatgccg gtggcgaggt gggtgatgcg caccgccgag tcggtcttgt
     1081 tgaccttctg gccgccggcg ccgccggcgc ggtaggtgtc gatgcgtatc tcgtcgggct
     1141 tgaccgtcac gtcgatgctg tcgtcgatca ccgggaaggc cgagacgctc gcgaagctcg
     1201 tgtgcctgcg cgcgttcgcg tcgaaggggc tgatgcgcac gagccggtgc acgccggact
     1261 cgccgcgcag gtggccgtag gcgtaggcgc cgtcgatctc catcgtcacg ctcttgatgc
     1321 cgccctcggc ctcgagcatg tcgacgatct cgtacttgaa gccacggcgc tccgcccatc
     1381 gggtgtacat gcgcatcagc atgcccgccc agtcgcaggc ctcggtgccg cccgagcccg
     1441 cgtggatgga caggaagacg ccggtgcggt cggcctcgcc ggtcatcagc tcgagcgtca
     1501 ccgccttgtc gaagcgatca cggacgccgg cgagcgacga ggctatttcc ccctcgaggg
     1561 agccctcgcc ctcctctatc gccatggcgt agatctcgtc gagggcatcg gtcgagcgct
     1621 tgatctcctc ccaggtctcg agccggttgc gcagcacctt gagctcgccc atgatgcgct
     1681 cggagcgctt ctggtcgttc cagaagccgg gctcggacga ctgggactcc ttcgtctcta
     1741 tcttggagcg tatggtagcg gcgtcaaaga cgcccccaga tttcaaggac ttctttcttg
     1801 agctgcgcta tcggcgccat gtagtcttca agcatggtct ctcctcgggc gttagggggc
     1861 cttctcggcc ggcgccctga cgacgacccg cttccatcga ttctccttga ggatcgagat
     1921 ggcgagccgt ccctcgacgg ggtattggta gatgcggacg gtgtcgtagt agtccgtcac
     1981 cttgtcgata tcgcgcttga gcgcgacgag ctgcttctcg gtggcgcgca cgctctcgaa
     2041 gcaggccctc tggaccttct cgaagccgta ctgaggcagg agcgcgtata cggcggccca
     2101 ggaatcctcg ccgccgaggt cgcaaacgac tgacacgaac atgagccatc gtatacgatg
     2161 ccgaagcgac gtgtcaaacc gaggataggg cgcgccatcg ctaccggcgc c
//
LOCUS       DAOOLN010000225         2211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_61633_length_2211_cov_2.820501, whole genome shotgun sequence.
ACCESSION   DAOOLN010000225 DAOOLN010000000
VERSION     DAOOLN010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61633_length_2211_cov_2.820501"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PLW80_03720"
     CDS             complement(<1..246)
                     /locus_tag="PLW80_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB65643.1"
                     /translation="MSNKKRILVVDDEQINLEFFDVMLSKLGFAVHKAENGQEALEAV
                     RRLRPDLIILDNIMPKLSGWEVTKIIKSSPEYAEFSEI"
     gene            complement(327..1982)
                     /gene="lnt"
                     /locus_tag="PLW80_03725"
     CDS             complement(327..1982)
                     /gene="lnt"
                     /locus_tag="PLW80_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HPB65644.1"
                     /translation="MQPKRRANSTAAVAPPPHLTSNLALLALTILLYTASFYSGSSLA
                     PFAAFFALVPAMAAAEGLGPLRSAIAGALSGAVALSIPCSWLASYHPVAITLVAFVGA
                     WSFSAAFAAVSAILRTGHPAAPLAVALVWASADIARSVGFLAFPYGSLPYALAGNRVA
                     LGLASIGGAPLVALAIAGANASCYAAARAIAHHGPGGRLRAALAALPAAAIVGAAVFG
                     AAPPFFSRPDDSLVVASPNPAGEPVAGSFRVALVQPCVYEQRSAPAYAAAFRKLAALS
                     VEAAERGADLVAWHETAVVPPIAWHLKQRPDRLTYEFVSDVDRFLSAYPVPLLVGNGY
                     ADPDDRARRVEHNSALLFVSGAAIARYDKAKLVPFSEYMPAWFGRALPSLDRRVIATF
                     GPYWTPGSEAGLIELGDARFAAPICFEDSFARYFASFEEPDFFIVLTKDSWASSASMQ
                     AQHLAMSRYRAAETGSIVLRVADTGSTAAIAPDGAVVGELAPFEPGVLYADVTLAAGT
                     RTAYEAGGMYFDLAIPLLAALLSTLCARRRPREGLRIDKKRSV"
     gene            complement(1957..>2211)
                     /locus_tag="PLW80_03730"
     CDS             complement(1957..>2211)
                     /locus_tag="PLW80_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPB65645.1"
                     /translation="DGLFEEIKKAILEEKAVELRGFGTFEVRIRKGRKRARNPKTGEP
                     VSVSDHGVAVFRPGRELKQEAWKISAIASPPPDAAEASGE"
BASE COUNT          404 a          663 c          777 g          367 t
ORIGIN      
        1 gatttccgag aattcggcgt actccggcga cgacttaatg atcttggtca cttcccagcc
       61 cgacaacttg ggcatgatgt tatcgaggat gatcaggtcg gggcgcaggc gcctgacggc
      121 ctcgagggct tcctgcccgt tctccgcctt atggaccgcg aacccgagct ttgacagcat
      181 tacatcgaag aactcgaggt tgatctgctc gtcgtcgacg acgagtatgc gttttttatt
      241 gctcacgtgg aaccccgttc gccgttctgg atcgatcgcc gagcgcgcga ggccatataa
      301 gccggcgcca tgatacggcc attttcctat acggatcgct tcttgtcaat gcgcaagccc
      361 tctcggggtc gtcgacgcgc gcagagcgtg gataggagcg cggcgaggag gggtatggca
      421 aggtcgaaat acatgccgcc ggcctcgtaa gcggtccggg tcccggccgc aagcgtcacg
      481 tcggcgtaca gcactccggg ctcgaacggc gccagctcac ccacgacggc gccgtccggc
      541 gcgatggccg cggtggagcc ggtatcggct actcgtagca cgatcgagcc ggtctcggcc
      601 gccctgtacc tcgacatagc gaggtgttgc gcctgcatcg aagccgaact agcccaggag
      661 tctttcgtga gtacgatgaa gaagtccggt tcttcgaatg aggcgaagta cctggcgaat
      721 gagtcctcga agcagatggg ggcggcgaag cgggcgtcgc ccagttcgat gagcccggct
      781 tcggagccgg gcgtccaata tgggccgaag gtcgctatga cgcgtcggtc gagcgacggc
      841 aaggctcgac cgaaccatgc cggcatgtac tccgagaacg gtacgagctt ggccttgtcg
      901 tacctggcta tggccgcccc ggagacgaag agcagggccg agttatgctc gacgcgcctg
      961 gcgcggtcgt cgggatcggc gtagccattc ccgaccagta gcggtacggg gtaggcagac
     1021 aggaatcgat cgacgtccga aacgaactcg taggttaggc gatctggcct ctgcttgagg
     1081 tgccaggcta tgggggggac gacggccgtt tcgtgccagg cgaccaggtc cgcgcctcgt
     1141 tcggccgctt cgacgctcaa ggctgctagc ttcctgaagg cggcggcgta ggccggcgcg
     1201 gatcgctgct cgtagacgca gggctgtacg agagcgaccc gaaacgatcc ggccaccggc
     1261 tcgcctgccg gattggggct cgcgacgacg agactatcgt ctggcctcga gaagaacgga
     1321 ggggccgcgc cgaacacggc ggctccgacg atggctgcgg ctggtagagc cgcaagcgcg
     1381 gcccgtaggc ggccacccgg accatggtgc gcgatggccc gagcggcggc gtagcagctg
     1441 gcgttagcgc cggcgatcgc cagagctacc agcggcgctc cgcctatcga ggcgaggccc
     1501 agggccactc ggttgccggc aagtgcgtag ggcaggctac cgtaggggaa ggccaggaag
     1561 ccgaccgatc tggctatgtc ggcgctcgcc cagacgagcg cgacggcgag cggcgccgca
     1621 gggtggccgg tacgcaggat ggcggagacg gcggcgaagg cggccgagaa cgaccacgca
     1681 ccgacgaagg cgacgagtgt tatcgctacg gggtggtacg aggcgagcca ggagcagggt
     1741 atcgatagcg cgacggcgcc cgacagggct ccggctatgg ccgaccgtag cgggccaagt
     1801 ccttctgccg cggccatagc tggtacgagg gcgaagaacg ccgcgaacgg cgcgagcgac
     1861 gagccgctgt agaacgaggc ggtatagaga agtatagtca gggcaaggag cgcgaggtta
     1921 ctggttagat gtgggggagg ggcgaccgcg gcggtactat tcgcccgacg cttcggctgc
     1981 atcgggcggt ggggaggcta tggcgctgat tttccaggcc tcctgcttga gctcgcgacc
     2041 gggcctgaaa acggccacgc catgatccga taccgatacg ggttcgccgg tcttgggatt
     2101 acgagcccgc ttacggccct tacggatgcg cacctcgaag gtaccgaacc cgcgcaactc
     2161 cacggccttc tcttctaaga tagccttctt gatctcttcg aacaggccgt c
//
LOCUS       DAOOLN010000226         2205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_61852_length_2205_cov_3.506977, whole genome shotgun sequence.
ACCESSION   DAOOLN010000226 DAOOLN010000000
VERSION     DAOOLN010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61852_length_2205_cov_3.506977"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PLW80_03735"
     CDS             <1..772
                     /locus_tag="PLW80_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HPB65646.1"
                     /translation="SKLLAQLVAAAFSNEKPDAEEIERLKVRSELDEMMGELADYLPS
                     VKEVLIDERDRYIASKIWTMRGERLVAVIGAGHAPGVEAWLARFAEGAPGGDTPGTDV
                     SDLELVPPPGLLARSAGWLVPALIVGFIALGFFTSGAEASLRLLLRWVALNGTLAAIG
                     SVLCLSHPLTVLVSFAAAPIATLNPLVAVGFFSGIVEAWLRKPQVSDFQTLSTDVSSL
                     KGFYRNKVTHILLVFFLSSLGGALGNFIALPFLAGGAF"
     gene            complement(769..1512)
                     /locus_tag="PLW80_03740"
     CDS             complement(769..1512)
                     /locus_tag="PLW80_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB65647.1"
                     /translation="MSAISVLLIEDEPGLVLTLTDLLSAKGYAVTNAADLASAEAAVA
                     SGGHDVILLDLMLPGGSGLDLLKAMRSRGDATPLLVLTAKADVTDRVEGLRLGADDYL
                     GKPFDSAELLARIEALVRRTKDGPARRRRTEAAVRFGDVEIDPERAEVRKAGIPVTLT
                     VQEFRLLMFLVENEGRTLSRACLLEEVWGYDGAIASRTVDVHISSLRQKLGDEPSDSR
                     WIVTIRGFGYRFDSNPQGEPRREPRREPR"
     gene            complement(1509..>2205)
                     /locus_tag="PLW80_03745"
     CDS             complement(1509..>2205)
                     /locus_tag="PLW80_03745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPB65648.1"
                     /translation="IRLTERERDFVASVTHELKTPVTVILSAGENLAEGIVEQRRVAE
                     YGAIVAKEARRLAETVERLLVMAGLQSTESVARSEPVEAADLIEAAIAKLRATASALV
                     VSFSVDIAGEPMIDGSALLLESALEGVVRNAIEYAGGAVRIRAYEKAVGKRAMVVIEC
                     SDDGPGVPKPERGQLFEPFYRGAAAIAARTKGTGIGLYLVRRVARMHGGDARLRFPPE
                     GGTIAELTFRSWR"
BASE COUNT          322 a          756 c          731 g          396 t
ORIGIN      
        1 ttcgaagctc ctggcccagc tcgtcgcggc cgccttctcg aacgagaagc ccgacgccga
       61 ggagatagag cggctcaagg tgcgctccga gctcgacgag atgatgggcg agctggccga
      121 ttacctgccg agcgtcaagg aagtgctcat cgacgagcgg gaccgctata tcgcctccaa
      181 gatctggacg atgcgcggcg agaggctggt agccgtgata ggggccggcc atgcgccggg
      241 cgtcgaagcc tggctcgccc gattcgccga aggcgcgcca ggcggcgaca ctccgggcac
      301 cgacgtatcg gacctcgagc tggtgccccc gcccggcctg ttggccagga gcgccggctg
      361 gctcgtaccg gccctgatcg tcggcttcat cgctctcggc ttctttacct ccggcgccga
      421 ggccagcctg cgcctcctcc tgcgctgggt ggcgctcaac ggcacgctgg cggccatcgg
      481 atccgtcctc tgcctgtccc acccgctgac ggtgctggta tcgttcgcgg cggcgcccat
      541 cgccaccctg aacccgctgg tcgcggtcgg ctttttttct ggaatcgtcg aggcctggct
      601 ccgcaagccc caggtgtccg atttccagac tctgtcgacc gacgtgtcga gcctgaaggg
      661 cttctatcgg aacaaggtca cgcacatcct gctggtcttc ttcctgtcga gccttggcgg
      721 cgcgctcggc aacttcatcg cgctaccttt cctggcgggc ggggcgttct agcggggttc
      781 ccttcggggt tcccttcggg gttccccttg ggggttcgaa tcaaaacggt agccaaagcc
      841 gcggatggtg acgatccagc gcgagtcgga cggttcgtcg ccgagttttt gcctgagcga
      901 cgaaatgtgt acgtcgaccg tcctcgaggc gatcgcgccg tcgtaacccc agacctcctc
      961 gagcaggcac gcacgggaca acgtgcggcc ctcgttctcg accaagaaca tcagtagccg
     1021 gaattcctgg acggtcagcg tgaccggtat cccggccttg cggacctcgg cccgttccgg
     1081 atcgatttca acgtcgccga agcgcactgc cgcctcggtc ctccggcggc gggccgggcc
     1141 atccttggtt cggcgcacga gcgcctcgat gcgggccagg agctccgccg agtcgaaggg
     1201 tttgccgagg tagtcgtcgg cgcccagcct gaggccctcg acgcggtcgg tcacgtcggc
     1261 cttggcggtc agcacgagca ggggcgtcgc gtcgcccctg gaccgcatcg ccttgagcag
     1321 gtcgaggccg gagcctccgg ggagcatcag gtcgagcagg atcacgtcgt ggccgcccga
     1381 ggcgacggcg gcctcggccg aggcgaggtc ggccgcgttc gtcacggcgt agcccttggc
     1441 cgagaggaga tccgtcaggg tcaggacgag accgggctcg tcctcgatca acagaaccga
     1501 gatagcgctc atcgccagct cctgaaggta agctcggcta tcgtgccgcc ttcgggcggg
     1561 aatcgtagcc gcgcgtctcc gccgtgcatg cgcgcgacgc gcctcacgag gtagaggccg
     1621 atgcccgtgc ctttggttct ggccgcgatg gctgcggcgc cgcggtagaa cggctcgaac
     1681 agctgcccgc gttcgggctt aggcactccc gggccgtcgt ccgagcattc tatcacgacc
     1741 atggcccgct tgccgaccgc cttctcgtag gcccgtatcc tcacggcgcc gccggcgtat
     1801 tcgatcgcgt tgcgcacgac gccctcgagg gccgactcga gtagcagggc cgagccgtct
     1861 atcatcggtt ctcccgctat atccaccgag aatgagacca cgagggccga ggctgtcgcc
     1921 cgtagcttgg ctatggcggc ttcgataagg tcggccgcct cgacgggctc gctcctggct
     1981 acggattcgg tcgactgaag gcccgccatg accagaagcc gttcgacggt ctcggccagc
     2041 cgcctagcct ccttggcgac gatggcgccg tactccgcga cgcgccgttg ctcgacgatg
     2101 ccctccgcga gattctcgcc ggcggacagg atcacggtga cgggcgtctt gagctcatgc
     2161 gtgacgctgg ccacgaaatc gcgttccctc tcggtcagcc gtatc
//
LOCUS       DAOOLN010000227         2203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_61937_length_2203_cov_3.792365, whole genome shotgun sequence.
ACCESSION   DAOOLN010000227 DAOOLN010000000
VERSION     DAOOLN010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61937_length_2203_cov_3.792365"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..655
                     /locus_tag="PLW80_03750"
                     /pseudo
     CDS             44..655
                     /locus_tag="PLW80_03750"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
     gene            750..2018
                     /gene="lysA"
                     /locus_tag="PLW80_03755"
     CDS             750..2018
                     /gene="lysA"
                     /locus_tag="PLW80_03755"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPB65649.1"
                     /translation="MTMAHLSFDGCDAVALAKEYGTPLYVVSERMIRERLRELREDFL
                     GRYPNTYALYASKALQTLDVLRIVASEGIGLDVVSGGELFAASKAGFPMDRVYFHGNS
                     KTDAEIRMAVEYGVGRVVVDNLHELETLNRAATALGRRARILFRVAPGVDSHTHEYIS
                     TGRLDSKFGIPLDRRVRDAYVGAALGMEGVELLGFHFHVGSQLLSNESHLKAIGVVCA
                     LMAELKADYGFETAELNLGGGYGVHYHDDERARPLSYFVDPMMAELSAACAAANLRLP
                     RVVIEPGRWVVGEAGLTLYTVGSKKVIPEVKTYVGVDGGLPDNPRPALYGAVYEASVA
                     DRHDAPRDEVVTVAGKCCESGDILIRDVALQSPEPGDVLAVFTTGAYNHSMASNYNRL
                     PRPAMVLTDSGEHRLSVRRETFDDLIAREL"
     gene            2020..>2203
                     /locus_tag="PLW80_03760"
     CDS             2020..>2203
                     /locus_tag="PLW80_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65650.1"
                     /translation="MRDEYADRGVPALHERLIVIDGHCDTAIPAAGYDFKSERVPPLD
                     LCARNDTGHVDFPRLLE"
BASE COUNT          349 a          805 c          706 g          343 t
ORIGIN      
        1 ctcgggcgtc acggatatcc tcgtctagga cgcccatggc tccggccgct cgatagaccc
       61 gtcgcgcctg ccgcgcgagg cccgcgtcct gcgcgcctgg acccgcgatc cctacgtgat
      121 gatggccggc ctcgagccga gccacgaggg cgtgttcttc atcggctacc actccgcggc
      181 cggcaccgac ggcagcccgc tcgctcatac tatggagacg aacaacgtgg ccgtcaggat
      241 caacggcgcg ctcgcctccg aattcctgat caacgcgtac atagccgcct acgtcggggt
      301 gcccgtcctg ctgctcgccg gcgacgccgc cctctgcgcc accgcggcct cggtcaatcc
      361 gaacatccgc accgtagccg tatccgaggg ccgcggcaac gcgtcgatat cgatacaccc
      421 ggctctggcc caggagcgca tcgcggccgc ggcggccgac gcgctcgcgg ccgacccgcg
      481 gcgcatgctc gtcgagctac ccgcgtcctt cgatgtgtcg atacacttca agcagcattg
      541 gctggccaac cggggctcgt actatccggg cgccaggcgc ttgggcgccc acgaggtagg
      601 cttctcgtcc aaggactggg gcgcggtgat ggccttcctg ttcttcgtgc tatgacccag
      661 gcgcgccgac gggccaaggc tccgtcggcg ctagccctag cgcctcggcc gtggtactct
      721 ttcctggccg acaggcgtga aaggggcgca tgaccatggc gcatctgagc ttcgacggct
      781 gcgacgccgt cgccctcgct aaggaatacg ggacccccct ctacgtggtc tccgaacgca
      841 tgatacgcga gcgcctgcgc gagctgcgcg aagacttcct gggacgctac ccgaacacct
      901 acgcgctgta cgcgagcaag gcgctccaga cgctcgacgt cctgcgcatc gtcgcctccg
      961 agggcatagg cctcgacgtg gtctccggcg gcgagctgtt cgcggcctcc aaggccggct
     1021 tcccgatgga ccgggtctat ttccacggca actccaagac cgacgccgag atcaggatgg
     1081 ccgtcgagta cggcgtcggc cgggtcgtcg tcgacaacct gcacgagctc gagacgctga
     1141 accgggccgc gacggccctg ggccgacgcg ccaggatact gttccgcgtc gccccgggcg
     1201 tggatagcca cacccacgag tacatctcga ccggtcggct cgactccaag ttcggcatcc
     1261 cgctcgaccg gcgcgtccgc gacgcttacg tcggcgcggc cctcggcatg gagggcgtcg
     1321 agctcctcgg cttccatttc cacgtcggct cccagctcct gtcgaacgaa tcccacctca
     1381 aggccatcgg cgtcgtctgc gcgctgatgg ccgagctcaa ggccgactac ggcttcgaga
     1441 cggccgagct gaacctcggc ggcggctacg gcgtccacta ccacgacgac gagcgggcca
     1501 ggcccttgtc gtacttcgtc gacccgatga tggccgagct gagcgcagcc tgcgcggccg
     1561 cgaacctgcg tctgccccgc gtcgtgatcg agcccggacg ctgggtagtc ggcgaggccg
     1621 gactcaccct gtacaccgtc ggctccaaga aggtcatccc ggaggtgaag acctacgtcg
     1681 gcgtcgacgg cggcctgccc gacaatccga ggccggccct gtacggcgcg gtctacgagg
     1741 cgagcgtggc cgaccggcac gacgcgccgc gcgacgaggt cgtcacggtc gcgggcaagt
     1801 gctgcgagtc gggcgacatc ctgatacgcg acgtcgcgct ccagtcgccc gagcccggcg
     1861 acgtgctcgc cgtgttcacg accggcgcct acaaccactc gatggccagc aactacaacc
     1921 gcctaccgcg cccggcgatg gtgttgaccg attccggcga gcaccgcctg tcggtccggc
     1981 gcgagacctt cgacgacctg atagccaggg agctgtgata tgcgcgacga atacgccgat
     2041 agaggagtcc ccgcgttaca cgaacgcctg atcgtcatcg acgggcactg cgacacggcc
     2101 ataccggccg ccggctacga cttcaagagc gaacgggtgc cgccgctcga cctctgcgct
     2161 cggaacgata cgggccacgt cgactttccc cggctgctcg agg
//
LOCUS       DAOOLN010000228         2200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_62069_length_2200_cov_3.541259, whole genome shotgun sequence.
ACCESSION   DAOOLN010000228 DAOOLN010000000
VERSION     DAOOLN010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62069_length_2200_cov_3.541259"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..1482
                     /locus_tag="PLW80_03765"
     CDS             88..1482
                     /locus_tag="PLW80_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB65651.1"
                     /translation="MLIKGGLCALPGSERLERRDLRISGERIVALAPSIDPEPGEIVL
                     DASALEVYPGAIDPHVHFDEPGFSHREDFRHGTMAAARGGVTTVIDMPCTSLPPVTSA
                     ANLREKLAVIGDKAVVDFALYGGVSGHLVEEALARDMEALAPDVVGFKCYFVSGMDTF
                     TAVSHYDFARAVAKAAELGRPLLLHAEDAGVVLPATAAMKARSAAEHREPSWADYVDS
                     RPESAETVAAAAAVALARGRESALHVVHVGTAEAAEAVASSGASCETCPHYLAFDSDD
                     FADKGSSLKTAPPVKAPGQADRLWRLLDSGVISYVTSDHAPAPAAEKRSGSVWTDYGG
                     IPGTGTLFPYLYSEGLRAGKLGLEAFLRATSGGAVARFGLSGGKGAIAVGKDADLVLV
                     DPVGSYVVRGDELLSKGSITPFEGMRLRGSIRLTMVRGRAVWDAETRERSADPAAGVV
                     VAAGYGKQLRWGYR"
     gene            1479..>2200
                     /locus_tag="PLW80_03770"
     CDS             1479..>2200
                     /locus_tag="PLW80_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="with YgfM and YgfN forms a selenate reductase,
                     which seems to catalyze the reduction of selenate to
                     selenite; YgfK mutants are unable to reduce selenate;
                     involved in purine salvage process; seems to act as an
                     oxidoreductase, but sequence similarity suggests it is a
                     dihydrothymine dehydrogenase; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HPB65652.1"
                     /translation="MSKIMKSMPMRELMARVVGEWTERDSAYDIPGALVRRVLEIESG
                     SPGFKVNGTAIGLPVGPAAGPHTQIAANIVAAWLAGARVFELKTVQENDRLDIEKPCI
                     DALDEGHNVEWSTELLLEEARAEYLNAWIAVHVLRAALCEKPGEIMFNMSVGYTLDGI
                     KGERVNAFIEGMRKPAGTPIWETALAEAKAAVVSPSFAAAFGEAGAKRARLVVEAMPS
                     APVHSVTLSTMHGCPPAEIERIG"
BASE COUNT          365 a          748 c          757 g          330 t
ORIGIN      
        1 ggaaggcaac cgcctgccga tgacgccgga gaaggccatg ctattcctga ccggcgacct
       61 cggcgtcgcg gtgaaggggc tatgaccatg cttatcaagg gaggcttatg cgcacttccg
      121 ggctccgagc ggctcgagcg gcgcgacctc aggatctccg gagaacgcat cgtcgcgcta
      181 gccccatcca tcgacccgga gcccggcgag atcgtgctcg acgcctcggc cctcgaggtc
      241 tacccgggag cgatagaccc gcacgtccac ttcgacgagc cgggcttctc gcatcgggaa
      301 gacttccgcc acgggacgat ggccgccgcc cgcggcggcg tgacgaccgt gatagacatg
      361 ccctgcacga gcctgccgcc ggtgacgagc gccgccaacc tgcgcgagaa gctcgcggtg
      421 atcggcgaca aggccgtcgt cgacttcgcg ctctacggcg gcgtgtccgg ccacctcgtc
      481 gaagaggccc tagcccgcga catggaggcg ctcgctcccg acgtggtcgg cttcaagtgc
      541 tacttcgtct ctggcatgga taccttcact gcggtcagcc attacgactt cgctcgagcc
      601 gtcgccaagg ccgccgagct cgggcgcccg ctcctcctcc acgccgagga cgcgggtgtc
      661 gtgctgcccg cgaccgccgc gatgaaggcc cgatccgctg ccgaacaccg ggagccgtct
      721 tgggccgact acgtcgactc gaggccggag agcgcggaga ccgtcgccgc ggcagccgcc
      781 gtcgccctgg cgcgaggccg cgagagcgcg ctgcatgtcg tccacgtcgg cacggcggaa
      841 gcggccgagg ccgtcgcctc gagcggcgcc agctgcgaga cctgcccgca ctatctggcc
      901 ttcgatagcg acgacttcgc cgacaagggc tcgtcgctca agacggcgcc gccggtcaag
      961 gcgccgggcc aggccgatag gctctggcgg ctcctcgact cgggcgtcat ttcgtacgtc
     1021 acgagcgacc acgctcccgc tccggcggca gaaaagcggt ccgggtcggt ctggaccgac
     1081 tacggcggca taccgggcac gggcacgctg ttcccgtacc tatactcgga ggggctcagg
     1141 gccggcaagc tcgggctcga ggccttcctg cgggctactt ccggtggagc cgtggccagg
     1201 ttcgggctgt ccggcggaaa aggcgccatc gccgtcggca aggacgccga cctggtcctc
     1261 gtcgacccgg taggatccta cgtcgtgcgc ggggatgagc tcctgagcaa gggttctatt
     1321 acgccgttcg aaggcatgcg ccttcgcggc tccatacgcc tgacgatggt tcgaggccga
     1381 gccgtatggg acgccgagac gcgagagcgc tcggccgacc cggctgccgg tgtcgtcgtc
     1441 gcggcaggat acggaaaaca actacgatgg gggtaccggt gagtaagatc atgaaatcca
     1501 tgccgatgcg cgaactgatg gctcgggtcg tcggagaatg gaccgagcgc gacagcgcct
     1561 acgacatccc aggggcgctc gtcaggcgcg tcctggagat agagtccggc tcgcccggct
     1621 tcaaggtgaa cggaacggcc atcggcttgc cggttggacc ggcggccggc ccgcacacgc
     1681 agatagccgc gaatatcgtg gcggcctggc tggccggtgc cagggtcttc gagctcaaga
     1741 ccgttcagga gaacgacagg ctcgacatcg agaagccatg catcgacgcc ctcgacgaag
     1801 ggcataacgt cgagtggtcg accgaactgc tactcgagga ggcccgcgcc gagtacctga
     1861 acgcctggat agccgtccac gtcctccggg ccgcgctctg cgagaagccc ggcgagatca
     1921 tgttcaatat gtcggtcggc tacaccctgg acggtatcaa gggcgagcgg gtgaacgcct
     1981 tcatcgaggg catgcggaag cccgcaggca ctcctatctg ggagaccgcg ctggcggagg
     2041 ccaaggccgc ggtcgtatcc ccgtccttcg ccgccgcgtt cggcgaggcc ggcgccaagc
     2101 gggccaggct cgtcgtcgag gcgatgccga gcgccccggt gcactcggtg accctatcga
     2161 cgatgcacgg ctgcccgccc gcggagatcg agcgtatcgg
//
LOCUS       DAOOLN010000229         2198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_62156_length_2198_cov_3.780215, whole genome shotgun sequence.
ACCESSION   DAOOLN010000229 DAOOLN010000000
VERSION     DAOOLN010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62156_length_2198_cov_3.780215"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PLW80_03775"
     CDS             complement(<1..217)
                     /locus_tag="PLW80_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65653.1"
                     /translation="MNLQVVVHGGVEAKLEQYALHAPKYLDRVLRLVGSQYRAMLKKN
                     YLSGQMIGRQTGDLYRSIYVSKVKSKKH"
     gene            complement(214..792)
                     /locus_tag="PLW80_03780"
     CDS             complement(214..792)
                     /locus_tag="PLW80_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65654.1"
                     /translation="MAVGEHALTSWKTAVSMFGYLDDEQTLVEKLIDQATARMELYTK
                     RKLAARDYDAVQLDGTGRDYLLLPEYPVNSIESVNLDSSRTFGDATLLDSSDYIIDSE
                     AGIIRLFSGVFPGYRGSVLVSYNAGFASTSQLYPMLEGGCLETVHWLKTRYAGGIGKR
                     TETNADGMSVGYEIDLPVNVRSMLDEIMRRDR"
     gene            complement(855..1280)
                     /locus_tag="PLW80_03785"
     CDS             complement(855..1280)
                     /locus_tag="PLW80_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65655.1"
                     /translation="MKDLHSQIDIVELIPAATYSADNTPAAVDLLGFESAEIALAIGA
                     GGITFTTSNKIEFVMTHSDDNSNYSNVTADDLLGVSSVTDGIVKSLVAAHATADVTRF
                     GYRGGKRYLKLLADFSGTHGTGTPLAAVVVKGHPHQATA"
     gene            complement(1347..>2198)
                     /locus_tag="PLW80_03790"
     CDS             complement(1347..>2198)
                     /locus_tag="PLW80_03790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HPB65656.1"
                     /translation="VPELEAGIDRVSMDQCVMRQLADVKTVGTSEAQLMVNKGGSGGG
                     WADEIGTRSETSTPSLDRISIPIRDMYANPLASQDLIDDAAFDVAAWLEDEAGIVFAE
                     LEGEAFISGALPTRPRGILSYTTVADASYAWGKVGYIASGKAGAWADSNPANAIISLQ
                     GALKGAYQNGASFIMNAAVMSDVRKFQTSYGYLWQPSFQAGTPQTLLGKPVYIDDNMP
                     AIAANTLSIAYGDWKRAYAIRDRQGIKVLRDPYSTKGFVEFYTTKRVGGGVRNFEAYK
                     VMKFAAS"
BASE COUNT          327 a          695 c          747 g          429 t
ORIGIN      
        1 catgcttctt cgacttcacc ttcgacacgt agatcgaccg gtagaggtcg ccggtctggc
       61 ggccgatcat ctgccccgac aggtagttct tcttgagcat ggcgcgatac tgcgatccga
      121 cgagccgcaa cacccggtcg aggtacttcg gggcgtggag cgcgtactgc tcgagcttcg
      181 cctcgacgcc gccgtggaca accacctgca ggttcatctg tcgcgcctca tgatctcgtc
      241 gagcatggag cggacgttga ccggtaggtc gatctcgtac ccgacgctca tgccgtcggc
      301 gttcgtctcg gtgcgcttcc cgatcccgcc ggcgtagcgg gtcttgagcc agtggacggt
      361 ctcaagacac ccgccctcga gcatcgggta gagctggctc gtgctcgcga atccggcgtt
      421 gtacgagacg agaaccgagc ctcggtatcc cgggaacacg ccagagaaca gcctgatgat
      481 gccggcctcg ctgtcgatga tgtagtcgct ggagtcgagg agcgtcgcgt cgccgaacgt
      541 acgcgagctg tcgaggttca ccgactcgat ggaattcacg gggtactcgg ggagcagaag
      601 gtagtcgcgg ccggtcccgt cgagctgtac ggcatcatag tcgcgggcgg cgagcttgcg
      661 cttcgtgtac agctccatcc gcgccgtcgc ctggtcgatg agcttctcga ccagcgtctg
      721 ctcgtcatcg aggtatccga acatgctgac cgcggttttc cacgaggtta gtgcgtgctc
      781 tccgaccgcc atatctatct ccctccgatg ccgctatgac ccgggagtgg tgagcctccc
      841 gggtcccgtg tgccttaggc cgtggcctgg tgcgggtggc ccttgaccac gaccgcggcg
      901 agcggcgtgc cggtgccgtg ggtgccggag aagtcggcga gcagcttcag gtagcgcttg
      961 cctcccctgt agccgaaccg cgtcacgtcc gccgtggcgt gcgccgcgac gaggctcttc
     1021 acgatgccat ccgtgaccga ggagacgccc aggaggtcgt cagcggtcac gttcgagtag
     1081 ttcgagttgt cgtcggagtg ggtcatcacg aactcgatct tgttgctggt cgtgaaggtg
     1141 ataccgccgg cgccgatggc cagggcgatc tccgcggact cgaagcccag gaggtctacc
     1201 gccgccggcg tgttgtcggc ggagtaggtc gcggccggga tcaactcgac gatgtctatc
     1261 tgcgagtgca ggtctttcat tcctctgtct cccttgcttg tcgtgccgag ggccatgcgg
     1321 cccccggcgg gttattcagc cggcgatcag ctcgcggcga acttcatgac cttgtaggcc
     1381 tcgaagttgc ggacgccgcc gccgacgcgc ttcgtggtgt agaactccac gaagcccttc
     1441 gtcgagtacg ggtccctgag taccttgatg ccctggcggt cgcggatggc gtacgcgcgc
     1501 ttccagtcgc cgtaggcgat cgagagcgtg ttcgcggcga tggccggcat gttgtcgtcg
     1561 atgtacacgg gctttccgag cagggtctgc ggggtcccgg cctggaacga cggctgccac
     1621 aggtagccgt acgacgtctg gaacttgcgg acgtcgctca tgacggccgc gttcatgatg
     1681 aacgacgcgc cgttctggta ggcgcccttg agcgcgccct gcaggctaat gatcgcgttg
     1741 gcggggttcg agtccgccca ggcgccggcc ttgccggaag cgatgtaccc gaccttgccc
     1801 cacgcgtagg aagcgtcggc aacggtggtg tacgacagga tgccgcgcgg tcgcgtcggg
     1861 agcgcgccgc tgatgaaggc ctcaccctcg agctcggcga atacgatgcc ggcttcgtcc
     1921 tcgagccagg cggccacgtc gaaggccgcg tcgtcgatca ggtcctgcga cgcgagcggg
     1981 ttcgcgtaca tgtcgcggat cgggatggag atacggtcga ggctcggggt gctggtttcg
     2041 gacctggtgc cgatctcgtc ggcccagcca ccgccggagc cgcccttgtt gaccatgagc
     2101 tgcgcctcgc tcgtgccgac ggtcttgacg tcggccagct ggcgcatgac gcactggtcc
     2161 atcgagacgc ggtcgatgcc ggcctcgagc tccgggac
//
LOCUS       DAOOLN010000230         2195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_62288_length_2195_cov_3.254673, whole genome shotgun sequence.
ACCESSION   DAOOLN010000230 DAOOLN010000000
VERSION     DAOOLN010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62288_length_2195_cov_3.254673"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="PLW80_03795"
     CDS             complement(<1..1200)
                     /locus_tag="PLW80_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPB65657.1"
                     /translation="MAKISSIKSGERRNATILFADLQGFTALSERMDPEEMDALMTKV
                     FGAFEEIIVSRGGVVEKYIGDALVAVFGVPELHEDDPQRALESALAFLERNRALEGEL
                     APRGATLSFRIGVHSGLIATGDRGSFKVVTGHPMAIAQRLQTAAEPDRVLVSDAVKER
                     CEADFDFEGPLRLAVKGASDELVAWRLVGQAQEGSRDQGPFVGRRDELEELLRLYVRH
                     DPRATSGMYLVGEAGIGKSRLAHALMERVRRFPEFGSPVLSAKAQRFRPLRYAVVVDL
                     VLDYLGVDFQSSPEAIASALAAQPGVERAHAELFASLLSPDARLRDSEIVLALFSIFS
                     ALMGRYERAVYPAFLFIDNAQAMDRQSTEFIAYYLKSAPCRPFALLAGREHGQSMRDA
                     FPELKALR"
     gene            complement(1212..>2195)
                     /locus_tag="PLW80_03800"
     CDS             complement(1212..>2195)
                     /locus_tag="PLW80_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65658.1"
                     /translation="ESLGGEIRFRTRAEALAIEGGRVRGVVLADGGRIDAGAVVLAIG
                     HSSRDTFASLAAQGVPMERKPFAIGLRIEHPQEMISRSQYGDAWDHPALGAADYKLTH
                     RASDGRSVYTFCMCPGGYVVDASSEPGLSACNGMSDYARDAPNANAAIVVSVRPDDFE
                     GSGPLAGVEYQRRWERLAWEAAREASGAPSAGHRGRSALPVQALGDFMAGRASACLGG
                     VTPSVRGSYALAELSACLPPFVASGIREAVPAFDRRLSGFGRTDAALCGVETRTSSPV
                     RVTRDDSLQSSLRGLFPCGEGAGYAGGIMSAAVDGVRVAEAISLSSAALLG"
BASE COUNT          334 a          771 c          791 g          299 t
ORIGIN      
        1 gcgcagcgcc ttgagctcgg ggaacgcgtc gcgcatcgac tggccgtgct cccgcccggc
       61 cagcagcgcg aagggccggc acggggcgga tttcaggtag tacgcgatga actccgtcga
      121 ttgccggtcc atcgcctggg cgttgtcgat gaacaggaag gccggataga cggcgcgctc
      181 gtagcgcccc atcagcgccg agaagatcga gaacagcgcc agcacgatct ccgagtccct
      241 gagccgggcg tccggcgata ggagcgaggc gaacagctcg gcgtgggccc gctcgacccc
      301 gggctgcgcg gccagggccg aggctatggc ctcggggctc gactggaagt cgacgcccag
      361 gtagtcgagc accaggtcga cgacgacggc gtagcgtagc ggcctgaagc gctgcgcctt
      421 ggccgacagc accggcgagc cgaactccgg gaagcggcgc acccgttcca tgagcgcgtg
      481 ggccaggcgg ctcttgccta tgccggcttc gccgaccaga tacatgcccg acgtcgcgcg
      541 cgggtcgtgc cgcacgtaga gcctgagcag ctcctcgagc tcgtcgcgcc ggccgacgaa
      601 gggcccctgg tcgcgcgagc cttcctgggc ctggccgacg aggcgccagg cgacgagctc
      661 gtcgctcgcg cccttgaccg ctagccggag cggcccctcg aagtcgaagt cggcctcgca
      721 gcgctccttg accgcgtccg agacgagcac ccggtccggc tcggcggcgg tttggaggcg
      781 ctgggctatg gccatcgggt gcccggtgac caccttgaac gagccgcggt cgccggtagc
      841 tatgaggccg gagtggacgc cgatcctgaa cgacagcgtc gccccgcgcg gcgcgagctc
      901 gccctcgagc gcgcggttcc gttcgaggaa ggcgagcgcc gactcgagcg cgcgctgcgg
      961 gtcgtcctcg tgcagctccg gtacgccgaa gaccgcgacg agggcgtcgc ctatgtactt
     1021 ctccacgacg ccgccccgcg agacgatgat ctcctcgaag gcgccgaaga ccttggtcat
     1081 cagcgcgtcc atctcctcgg gatccatgcg ctccgacagc gcggtgaagc cctggagatc
     1141 ggcgaagagt atggtcgcgt tcctgcgctc gcccgatttt atggagctga tcttggccat
     1201 ggcccgatac tctagcctaa aagcgccgcg cttgaaagcg atatggcctc ggccacccgt
     1261 acgccgtcga cggccgccga catgatgccg ccggcgtagc cggctccctc gccgcaggga
     1321 aacagcccgc gtagcgacga ctgcagggag tcgtccctgg ttacgcggac gggcgacgag
     1381 gtgcgcgtct cgacgccgca gagcgccgcg tcggtcctgc cgaagccgga cagcctgcga
     1441 tcgaaggcgg gcacggcctc ccgtatgccc gacgcgacga agggcggtag gcaggcgctt
     1501 agctccgcca gcgcgtagga gccgcggacg gacggcgtca cgccgcccag gcaggccgac
     1561 gcccggcccg ccatgaagtc gccgagggcc tggaccggca gggcggacct cccgcgatgg
     1621 ccggccgaag gggcgcccga cgcctcgcgc gcggcctccc aggccagccg ttcccagcgc
     1681 cgctggtact cgacgccggc cagggggccc gagccttcga agtcgtcggg gcgcaccgat
     1741 acgacgatcg cggcgttcgc gttcggcgca tcgcgggcgt agtcgctcat gccgttgcag
     1801 gcggataggc caggctccga actggcgtcg accacgtagc cgccggggca catgcagaac
     1861 gtgtagacgc tccgcccgtc cgaggcgcgg tgggtcagct tgtagtcggc ggcgccgagg
     1921 gccggatggt cccaggcatc gccgtactgc gagcgcgata tcatttcctg cgggtgctcg
     1981 atgcgcaggc ctatggcgaa gggcttgcgc tccatcggca cgccctgggc ggcgagcgac
     2041 gcgaaggtat cgcgcgacga gtggccgatg gccagcacga cggcgcccgc gtcgatacgg
     2101 cccccgtcgg ccaggacgac gccgcgtacg cgtccgccct cgatagcgag ggcctcggcg
     2161 cgggtcctga agcgtatctc gccgccgagc gactc
//
LOCUS       DAOOLN010000231         2195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_62290_length_2195_cov_3.169159, whole genome shotgun sequence.
ACCESSION   DAOOLN010000231 DAOOLN010000000
VERSION     DAOOLN010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62290_length_2195_cov_3.169159"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="PLW80_03805"
     CDS             <1..767
                     /locus_tag="PLW80_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPB65659.1"
                     /translation="RLSKAERLIYQHGDFDDLEAKLKAAAGKRLRLVATDGVFSMDGD
                     IADLERICDLAERYDALVMVDDSHATGFIGKRGRGTHEYRGVMDRVDIITTTFGKALG
                     GASGGCVAGKAEIVEYLRQKARPYLFSNTLAPAIVGGTLKALELLTESTELRDRLESN
                     TKRFRDGLARAGLDVRPGVHPICPVMLYDERLAHEMADRLLDEGIYVIGFSFPVVPRG
                     KARIRVQVSAAHSVEQIDAAVAAFAKVGAAMGAVKK"
     gene            777..1196
                     /locus_tag="PLW80_03810"
     CDS             777..1196
                     /locus_tag="PLW80_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65660.1"
                     /translation="MTKPRLVIVLSAGAVLAVVVAVVVGVSLYSRSEAARESLVVRYF
                     RALSSGDEALVKDLVAPEFSSDLGLGNLVRGSYELFDFGEREPGTLRFLIIVQGEAGG
                     SGAERRAMLCELRYRRYGLTNRIGSIRLVESGVGLKE"
     gene            complement(1209..>2195)
                     /gene="tsaD"
                     /locus_tag="PLW80_03815"
     CDS             complement(1209..>2195)
                     /gene="tsaD"
                     /locus_tag="PLW80_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPB65661.1"
                     /translation="GRSATVTVLFSDIVGSTEHLHRLGERSWDEVRREHFAVLRTSLA
                     EHNGTEVKNTGDGLMAVFDSAIDGVAVTARPGLAGSLLVGVSFAKAFAWSRGLPFIGV
                     NHVLAHLYAPLLDDEVEYPFIGLIVSGGHTLICRADGFDDVTVLGTTIDDAIGEAFDK
                     VAKHYGLGYPGGSAIDRLARAGDAAACDFPRPNLYKGEHRYDVSYSGLKTAAIRQLDQ
                     FWNPAYERNDANLAAAFERAAVDMLVSRLLRALDDTGIRTVVAGGGVAANTYLRSRLA
                     GRAGLRVLFPPLSLCGDNAAMVAGLGYRMLERGDRDGLQLNASPRVAAFKRS"
BASE COUNT          352 a          729 c          752 g          362 t
ORIGIN      
        1 tgcgcctgtc caaggccgag cgcctgatct accagcacgg cgacttcgac gacctcgagg
       61 ccaagctcaa ggccgccgcc ggcaagcgtc tgcgactggt cgcgaccgac ggcgtcttct
      121 cgatggacgg cgacatcgcc gacctcgagc gcatctgcga cctggccgag cgctatgacg
      181 ccctcgtgat ggtcgacgac agccacgcga ccggcttcat cggcaagcgc ggccgcggca
      241 cccacgagta ccgcggcgtg atggatcgcg tcgacatcat cacgacgacc ttcggcaagg
      301 ccctcggcgg cgcttccggc ggctgcgtcg ccggcaaggc cgagatcgtc gagtatctca
      361 ggcagaaggc cagaccctac ctgttctcca acacgctcgc ccctgccata gtcggcggca
      421 ctctcaaggc tctcgagcta ctgacggagt cgaccgagct gcgcgatagg ctcgagtcga
      481 acaccaagcg cttcagggac ggcctggcca gggccggcct cgacgtacgt ccgggcgtgc
      541 accccatctg cccggttatg ctttacgacg agcgcctcgc ccacgagatg gccgaccgcc
      601 tgctcgacga gggcatctac gtgataggct tcagcttccc ggtcgtgccc aggggcaagg
      661 ccaggatccg tgtccaggta tccgcggccc actcggtcga gcagatcgac gccgcggtgg
      721 cggccttcgc caaggtcggt gccgcgatgg gcgccgtcaa gaagtagggt agttcgatga
      781 ccaagccgag gctcgtcatc gtcctatccg cgggggccgt cctggccgtc gtggtcgcgg
      841 tcgtcgtggg cgtcagcctg tactcgcgta gcgaagcggc tagggaatcg ctcgtcgtcc
      901 gctacttcag ggccctgtcc tcgggcgatg aagcgttggt caaggatctc gtagcgccgg
      961 aattctcatc cgacctaggc ctcgggaacc tggttcgagg aagctacgag ctgttcgact
     1021 tcggcgagag ggagcctgga accctgcgtt tcctgatcat cgtgcaaggc gaggctggcg
     1081 gctcgggggc cgagcggcgg gccatgctct gcgagctacg ctatcgccgt tacggcctga
     1141 ccaatcggat cggctcgatc aggctcgtag agtctggcgt cgggctcaag gagtagggcg
     1201 gggcctcgct aggatcgctt gaacgcggcg acgcgcggcg aggcgttgag ctggaggccg
     1261 tccctgtcgc cgcgctccag catgcggtag ccgaggccgg cgaccatggc ggcgttgtcg
     1321 ccgcagagcg acagtggcgg gaagagtacg cgtagcccag ccctgccggc caggcgcgat
     1381 cgcaggtagg tgttcgccgc gacgccgccg ccggcgacca ccgtgcgtat gcccgtgtcg
     1441 tccagcgctc gcagtagcct cgagacgagc atgtcgacgg ccgccctctc gaaggccgcg
     1501 gccaggttgg cgtcgttgcg ctcgtaggcg gggttccaga actgatcgag ctggcgtatg
     1561 gcggccgtct tgagtccaga gtacgataca tcgtagcggt gctcgccctt gtacaggttc
     1621 ggccttggga agtcgcaggc cgcggcatcg cccgcgcgcg ccagtcggtc gatggcgcta
     1681 ccgcccgggt agccgaggcc gtagtgcttg gccaccttat cgaaggcctc tccgatggcg
     1741 tcgtcgatgg tcgtgcccag cacggttacg tcgtcgaaac cgtcggcccg gcatatcagc
     1801 gtgtggccgc cggagacgat caggccgatg aatggatact cgacctcgtc atcgaggagc
     1861 ggcgcgtaga ggtgggccag cacatggttc acgccgataa aaggcagacc tcgcgaccag
     1921 gcgaacgcct tggcgaagga tacgccgacg agcagggagc cggcgaggcc gggcctggcc
     1981 gttacggcca cgccgtcgat ggcgctgtcg aagaccgcca tcaacccgtc gccggtgttc
     2041 ttgacctcgg tgccgttgtg ctcggcgagc gacgtccgga ggaccgcgaa gtgctcgcgt
     2101 cggacctcgt cccacgaccg ctcccccagt cgatggaggt gctcggtcga cccgacgatg
     2161 tcgctgaaca acaccgtcac cgtcgcgctc cgccc
//
LOCUS       DAOOLN010000232         2191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_62441_length_2191_cov_3.800562, whole genome shotgun sequence.
ACCESSION   DAOOLN010000232 DAOOLN010000000
VERSION     DAOOLN010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62441_length_2191_cov_3.800562"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PLW80_03820"
     CDS             complement(<1..437)
                     /locus_tag="PLW80_03820"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HPB65662.1"
                     /translation="MAELAMADRDYAPPRALGYPGEYPGERARDGRSAGHSTSRPSSR
                     TMARNAGGVSARAAGGPRLAVAIASTLLAIAAVAGVAAFALPRTEVRGLTVSGAVTIG
                     ERELYAWSGLSERQHWFSVDRGAVAACLSAHPRVASVSVETRFP"
     gene            complement(430..1557)
                     /locus_tag="PLW80_03825"
     CDS             complement(430..1557)
                     /locus_tag="PLW80_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HPB65663.1"
                     /translation="MYGGFMMEKPLGRVGGDPGMLVSLALLVGVGLACLYSASYGFAL
                     SLGKPAGYFAVRQLIWMAPAAAIFAFGAFLPLDAARRWIGVAVIGSLAMLVLPFVPGI
                     GITKNGASRWFGFGGQMFQPSELFKPVIVLYLAHILSKKGEKLGDVVNGVMPPLIVVM
                     AGALIVLAQNDFSTAALVITLAVVMFWISGVPLSFFAAIGSVGLPLVALSVLTSDYRL
                     KRVIGFVAPGYDPADLSYQVNGSLRAIRSGGFWGKGIGQGSLKLRSIPEVQSDFVFAA
                     WVEETGFAGVLAFAALWAVFLWRAYRVAFRSEDTFRSHLAFGIACYLGIQTLLNVMVA
                     SGAAPATGIPLPFFSSGGSSLVSAALSAGLLFNVSRSVNHG"
     gene            complement(1569..>2191)
                     /gene="mraY"
                     /locus_tag="PLW80_03830"
     CDS             complement(1569..>2191)
                     /gene="mraY"
                     /locus_tag="PLW80_03830"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPB65664.1"
                     /translation="EMTKLYVPFFKAPVVDLGIFWIPLAVVYVMGWSNAVNLSDGLDG
                     LATGLVIMALIAFSLLTYLTGRADWSEYLGIPFVRGSGELTVFCLALLGACVGFLWFN
                     AHPAEVFMGDVGSLSLGGVLAVLALIVKKELLFFIIGGVFVLEEASVAIQVLYFKATK
                     GKRVFRMAPLHHHFELSGWKETKVVTRFWILGGLFAIIALSTLKIQ"
BASE COUNT          461 a          735 c          712 g          283 t
ORIGIN      
        1 ggaaaacgcg tctcgaccga tacgctggcc acgcgcgggt gggccgacag gcaggcggcc
       61 acggccccgc ggtcgaccga gaaccaatgc tggcgctccg aaaggccgga ccacgcgtac
      121 agctcgcgct cccctatcgt gacggccccg gatacggtga ggcccctcac ctcggtgcgt
      181 ggtaacgcga aggccgctac gccggccaca gcggctatcg ccagcaaggt cgacgcgatg
      241 gccacggcca gcctcgggcc cccggccgca cgggccgaga cgccaccggc gttcctggcc
      301 atcgtgcgcg acgagggtcg gctcgtggag tggcccgcgg agcgaccgtc ccgagcgcgc
      361 tcgcccgggt actcgcccgg gtagcctagc gccctcggag gggcgtaatc cctgtccgcc
      421 atcgcaagct cagccatggt ttacgctcct cgaaacatta aatagaagcc cggcgctaag
      481 cgcggcggac acgagcgaag agccgcccga cgagaagaac ggtagcggga tacctgtagc
      541 cggagccgcg cccgaggcca ccatcacatt gaggagcgtc tgtatgccta aatagcaagc
      601 gatcccgaag gccaggtggc tccggaacgt atcctcggaa cggaatgcga cccggtacgc
      661 cctccagagg aataccgccc acagggcggc gaaggccagt acgcccgcga agccggtctc
      721 ctcgacccag gccgcgaaga cgaagtccga ctgcacctcg ggtatgctcc tgagcttgag
      781 cgaaccctgg ccgatgccct tgccccagaa gcctcccgag cggatggcgc gaagggagcc
      841 gttcacctgg tacgacaggt ccgccgggtc gtagcccggc gccacgaagc cgatcacgcg
      901 tttgagcctg tagtcgctcg tcaacaccga caaggcgacg agcgggaggc cgaccgagcc
      961 gatggcggcg aagaacgaca gcggcacccc ggagatccag aacatgacga cggccagggt
     1021 aatgacgagc gccgcggtcg agaagtcatt ctgggccagc acgatcaggg cgccggccat
     1081 caccacgatc agcggaggca tcacgccgtt gacgacgtcg ccgagcttct cgcccttctt
     1141 ggaaagaata tgggccaggt agagcacgat gaccggcttg aacagctccg acggttggaa
     1201 catctgcccg ccgaagccga accagcgaga ggcgccattc ttggtaatgc cgatgccggg
     1261 cacgaagggc aggacgagca tggccaggct gccgatcacg gcgacgccta tccagcgtcg
     1321 agccgcgtcc aggggcagaa aggccccgaa cgcgaagatg gcggcggccg gcgccatcca
     1381 gatcagctgc cgtacggcga aatacccggc cggcttgccg agcgacaggg cgaagccgta
     1441 cgacgcggaa tagagacagg ccaagccgac gccgaccagc agcgcgagcg agacgagcat
     1501 cccggggtct cccccgacac ggccgagggg cttctccatc atgaatccgc cgtacatggc
     1561 tccgtctcct actgtatctt gagcgtcgac agcgcgatga tcgcgaacag cccgccgagt
     1621 atccagaacc gcgtgaccac cttggtctcc ttccagccgg acagctcgaa gtggtggtgg
     1681 agcggcgcca tgcggaaaac ccgcttgccc ttggtcgcct tgaagtacag cacctggatg
     1741 gctaccgaag cctcctcgag gacgaatacg ccgccgatga tgaagaacag cagctctttc
     1801 ttgacgatca gggccagcac ggccagcacg cccccgaggc tcagcgagcc gacgtcgccc
     1861 atgaatacct cggcagggtg agcattgaac cagaggaaac cgacgcaggc gccgagtagg
     1921 gccaggcaga acaccgtcag ctccccggag cctcggacga agggtatgcc caggtactcg
     1981 gaccaatcgg cgcggccggt caggtaggtc aggagcgaaa aggcgatcag cgccatgatc
     2041 acgaggccgg tcgccaggcc gtccaagcca tcggacaggt tgaccgcgtt cgaccagccc
     2101 atcacgtaga ccactgcgag cgggatccag aatatcccca agtcgacgac gggcgccttg
     2161 aagaacggca cgtacagctt ggtcatttcg c
//
LOCUS       DAOOLN010000233         2189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_62557_length_2189_cov_2.490159, whole genome shotgun sequence.
ACCESSION   DAOOLN010000233 DAOOLN010000000
VERSION     DAOOLN010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62557_length_2189_cov_2.490159"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..1654
                     /locus_tag="PLW80_03835"
     CDS             122..1654
                     /locus_tag="PLW80_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPB65665.1"
                     /translation="MRIARRGGSASLAPAAFLTLAGASAPAQGVYVVPIHGDIAASTV
                     VFVRRQATKAIGDGAAAIIFDIDTFGGRVDSALRISSFIGSIREADTVAYVRSGPDGL
                     GVSWSAGALIAMSCGSLYMAPGTSIGAAAPVVAGADGSTTRADEKTVSAVRAQMAALA
                     EKNGYPPAIALAMVDADVELYEVRRDGAVALATADELGLEKGADGVPVDPASGERLRL
                     VSAKGKLLSLTAGEAERYGLSFGTAEGLENLLEAVGLEGPAIELTPSVADGLVVFLSS
                     AAVQSLLILVGLVAVFIEINSPGFGIPGTVALVAFMTLFGVNALMGTVGSLELVLFML
                     GAALLAVELFLIPGFGITGIAGILLIAVSLVLSMQDFALPTLDWEWELLGRNALTVAV
                     GILSAIAGIGVLALAGPRLRMFDRLTLHTTIGGTAGGQSLPPAVPEAPETSPAAADSL
                     AGRRGVALTTLRPSGRAIIDGGTYSVETDGTYVEAGAALRVDSVRGSRILVSVRTPDK
                     ES"
     gene            1656..>2189
                     /locus_tag="PLW80_03840"
     CDS             1656..>2189
                     /locus_tag="PLW80_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like FloA family protein"
                     /protein_id="HPB65666.1"
                     /translation="MLTVALYALVGLVALGVLFMFFKFFGLWFRALLSGAPVGMGRLI
                     GMWLRHVKPGIIVDARIMLSKAGLDVNSDMLETHFLARGNVFKVSQALVAANKADIPL
                     PFQRAAAIDLAGRDVLEAVKTSVNPKVIDCPDPGKGKNTIDAVAKDGIQLQVKARVTV
                     RANIERLVGGATEETIIA"
BASE COUNT          325 a          785 c          727 g          352 t
ORIGIN      
        1 agctcgaccg acggcaccgg tcccgccagc gccccggtga tgcacggcat tccctgcgcc
       61 ttaaagaaga tccgcccgac cgtcccttga ggttcagccg gaagcgacta tagtaaaagg
      121 catgagaata gctcgtcgcg gcggttcggc gtctctggcg ccggccgcgt tcctaacctt
      181 ggccggagcc tccgccccgg cccagggggt ctatgtcgtc ccgatccacg gcgatatagc
      241 agcgtccacg gtcgtcttcg tccgccgcca ggcgacgaag gccatcggcg atggggccgc
      301 ggccatcatc ttcgacatcg ataccttcgg cggccgcgtc gactccgccc tacgcatctc
      361 gtcgttcata ggctcgatcc gcgaggccga caccgtggcc tacgtccgct ccggccccga
      421 cggcctcggc gtctcgtggt cggccggggc gctcatcgcc atgtcctgcg gctcgctgta
      481 catggcgccg ggcacatcga taggggcggc cgccccggtg gtcgcgggcg cggacgggtc
      541 gacaacacgg gccgacgaga agaccgtcag cgccgtgcga gcccagatgg ccgccctggc
      601 cgagaagaac ggctatcccc cggccatcgc cctcgcgatg gtcgacgcgg acgtggaact
      661 ctacgaggtc cggagggacg gggcggtcgc gctggccacc gcggacgagc taggcctcga
      721 gaagggagcc gacggcgtcc ccgtggaccc ggcctctggc gagaggctca ggctcgtcag
      781 cgccaagggc aagctcctgt cgcttaccgc cggcgaggcg gagcgctacg gcctatcgtt
      841 cggcaccgcc gaggggctcg agaacctgct ggaggccgtc ggcctcgagg ggccggccat
      901 cgagctgacc ccgagcgtcg cggatgggct cgtcgtcttc ctgagctcgg cggccgtcca
      961 gtcgctgctg atactcgtcg gcctcgtggc cgtgttcatc gagatcaact cgcccggttt
     1021 cgggataccc ggcaccgtgg cgctcgtggc cttcatgacg ctgttcggcg tgaacgccct
     1081 gatgggcacg gtcggctcgc tcgagctggt gctcttcatg ctcggcgcgg ccctgttggc
     1141 cgtggagctc ttcctgatac cgggcttcgg tatcaccggc atcgccggca tcctgctcat
     1201 cgccgtctcg ctcgtgctgt cgatgcagga tttcgcgttg ccgaccctcg actgggaatg
     1261 ggagctgctc gggcgcaacg cgctcacggt ggcggtcggc atcctgtcgg ccatcgccgg
     1321 cataggcgtg ctcgccctcg ccggacctcg cctgcggatg ttcgaccggc tgaccctgca
     1381 cacgacgata ggcggcaccg ccggcgggca gtcgctcccg cccgccgtcc ccgaggcccc
     1441 ggaaacctca ccagccgcgg ccgacagcct cgcaggcagg cgcggcgtcg cgctgaccac
     1501 cctccggccg tcgggccgcg ccatcatcga cggcgggacc tacagcgtcg aaacggacgg
     1561 gacctacgtc gaggcgggcg ccgcgctccg cgtcgactcg gtccgcggct cgaggatact
     1621 cgtatcggta cgaacgccag acaaggagtc gtgacatgct gaccgtagcc ctgtacgcgc
     1681 tcgtgggcct cgtcgccctc ggcgtcctgt tcatgttctt caagttcttc ggcctgtggt
     1741 tcagggccct gctgtccgga gcgcccgtcg gcatgggccg cctgatcggc atgtggctca
     1801 ggcacgtcaa gccgggcatc atcgtcgacg cgcgcatcat gctctcgaag gctggcctcg
     1861 acgttaattc cgacatgctg gagacgcatt tcctcgcgag gggcaacgta ttcaaggtca
     1921 gccaggccct cgtcgcggcc aacaaggcgg acatccccct gcccttccag cgcgcggccg
     1981 cgatagacct ggccggccgc gacgtgctcg aggcggtcaa gaccagcgtc aaccccaagg
     2041 tgatagactg ccccgacccc ggcaaaggca agaacacgat agacgcggtg gccaaggacg
     2101 gcatccagct ccaggtcaag gcccgcgtca ccgtgcgcgc caacatcgag cgcctcgtcg
     2161 gcggcgccac cgaggagacc atcatcgcc
//
LOCUS       DAOOLN010000234         2183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_62789_length_2183_cov_3.680921, whole genome shotgun sequence.
ACCESSION   DAOOLN010000234 DAOOLN010000000
VERSION     DAOOLN010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62789_length_2183_cov_3.680921"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PLW80_03845"
     CDS             complement(<1..190)
                     /locus_tag="PLW80_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65667.1"
                     /translation="MVYDYDLHTIADIAPIGAAEFRDRPALGLVGGEHVDYRELELQS
                     RRVAADLYRRGVRKGDRVA"
     gene            complement(241..861)
                     /locus_tag="PLW80_03850"
     CDS             complement(241..861)
                     /locus_tag="PLW80_03850"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPB65668.1"
                     /translation="MAFNVPELMYPYDALEPWIDEATMRVHHDKHHAAYVNNLDKALA
                     GSEYAAWPVERLIAELDSLPESLRTTVRNHGGGHYNHTLFWTMMTPALMGAPAKPDGA
                     LAAALDRDLGGFDAFREAFGKAAAGRFGSGWAWLVVGKDGRLAVSSTQNQDNPLTNGL
                     RPILGLDVWEHAYYLKYQNRRQEYIDNFFNVIAWKEVSRRFDAANT"
     gene            1197..>2183
                     /locus_tag="PLW80_03855"
     CDS             1197..>2183
                     /locus_tag="PLW80_03855"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPB65669.1"
                     /translation="MASDAQKKVLVLNSGSSSLKYEVFLMPKRESLGKGLVERIGEAK
                     GYLTQSSPKGSIRIDEVFPDHKHAMLRVGQALVDPLKGILGSIDEIEAIGHRVVHGGE
                     RFASSVRIDEAVVKALEDNVELAPLHNPANLTGIAEAEQLWPGVPQVAVFDTAFHQTM
                     APTAYLYGLPRELYDKYRIRRYGFHGTSHRFVARRALELLGRRAENTNLITCHLGNGA
                     SITAIEGGRSIDTSMGFTPLEGLMMGTRSGDFDPAILGFLVDRGYSPQELTTMINKKS
                     GLLGLSGISNDLRDLEAAAEKGNRNAIEALDVYAHRVRQYIGAYMAELVKVDA"
BASE COUNT          440 a          695 c          632 g          416 t
ORIGIN      
        1 tggccacgcg gtcgccctta cggacgccgc ggcgatacag gtcggcggcc acgcgcctcg
       61 actggagctc gagctcgcga tagtcgacgt gttccccacc gacgaggccg agcgccggcc
      121 tgtccctaaa ttccgccgcc ccgatcgggg cgatgtcggc gatcgtatgt aggtcgtagt
      181 cgtataccat cttggatcct cggcgagtta gaccgccggg atccaggatg gttgcatggt
      241 ttaggtattc gcagcgtcga atcgcctgga gacctccttc caggcgatca cgttgaagaa
      301 gttatcgatg tactcctgcc tgcggttctg gtacttaagg tagtacgcgt gctcccagac
      361 atccaggccg agtatagggc gtaggccgtt cgtgagcggg ttgtcctggt tctgagtcga
      421 cgagacagcc agcctaccgt ccttgccgac gacgagccag gcccagccgg agccgaaccg
      481 gcctgccgcc gccttgccga aggcttccct gaacgcgtcg aagccgccca ggtccctatc
      541 cagggccgcg gccagggcgc cgtcgggttt ggccggcgcg cccatgaggg ccggcgtcat
      601 catggtccag aacagcgtat ggttgtagtg tccgccgccg tggttgcgga ccgtcgtccg
      661 caggctctcg ggcaagctat cgagctcggc gatcagccgc tccaccggcc aggcagcgta
      721 ctcgctgccg gctagcgctt tatcgagatt gttcacatag gccgcatggt gcttgtcgtg
      781 atgcacccgc atcgtcgcct cgtcgatcca gggctcgagc gcgtcatagg gatacataag
      841 ctcgggtacg ttaaaagcca tggggggctc cttgggacta tcgatgttac cccgaaaggt
      901 acattactat attagtaatg tttaatacac aaggcaatgt acctaagtgg tacaaagctg
      961 agtggtcctc atgcaactaa ttcttatttt ggtaagaatt gctttcatca taacggcagg
     1021 tcacggtcgg agattccaga ggttagtccg aacagcaaac acggactcga ttcgtcaata
     1081 cggttcatat ctctacagat gatatcgatt gatgaaacga tcatattggc ttatgatcca
     1141 ttcgtcggcc ttctacccgt tcatcaggca tcgaccgacg gaagggacga ataataatgg
     1201 cttcagacgc tcagaagaaa gtgctcgtgc tcaatagcgg cagctcatcg ctcaaatacg
     1261 aggtgttcct catgccaaag cgtgaaagcc tcggcaaggg actcgtcgag cgcatcggtg
     1321 aagccaaagg ctacttgacc cagagttcgc ctaaaggctc gatcaggatc gacgaggtgt
     1381 tccccgacca taaacacgcg atgctccgcg tcgggcaggc cctcgtagat ccgctcaagg
     1441 gcatactcgg ctccatcgac gagatcgagg ccatcggaca ccgcgtcgtg cacggcggcg
     1501 agcgcttcgc aagctccgtc cgtatcgacg aggccgtcgt caaggcgctc gaagacaacg
     1561 tcgaactggc gccgctccac aatcccgcca acctgaccgg tatagccgaa gccgagcagc
     1621 tatggcccgg cgtccctcag gtcgccgtct tcgacaccgc attccaccag accatggcgc
     1681 ccacggccta cctgtacggc ctaccccgcg agctatacga caagtaccgt atcaggcgct
     1741 acggcttcca cggcacgagc caccgcttcg tggccaggcg cgccctcgag ctactcggcc
     1801 gcagagccga gaacaccaac ctgatcacct gccacctcgg caacggcgcg tcgatcaccg
     1861 ccatcgaagg cggccgctcc atcgacacgt ccatgggctt cacgccgctc gagggactga
     1921 tgatgggcac ccgctccggc gacttcgacc cggccatctt gggcttcctc gtcgatcgcg
     1981 gctattcgcc tcaggaactg acgacgatga tcaacaagaa gagcggcctc ctcggcctct
     2041 ccggcatctc caacgacctg cgcgacctgg aggccgccgc cgagaagggc aaccgcaacg
     2101 ctatcgaggc gctcgacgtc tacgcccacc gcgtgcgcca gtatatcggc gcctatatgg
     2161 ccgagctggt caaggtcgac gcg
//
LOCUS       DAOOLN010000235         2180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_62930_length_2180_cov_3.687059, whole genome shotgun sequence.
ACCESSION   DAOOLN010000235 DAOOLN010000000
VERSION     DAOOLN010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62930_length_2180_cov_3.687059"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /locus_tag="PLW80_03860"
     CDS             <1..1295
                     /locus_tag="PLW80_03860"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HPB65670.1"
                     /translation="GPGSLPTIDRAYLRVMRRKYDQATKLPAELVSEMARATSLSQAA
                     WVDARKHNDFKAFAPHLSKMLEYNKKVAACLDPSRKPYDVLLDRFEEGASEEQVASIF
                     GPLREELVSLLDRIRGKPQIDDSFLHRHCPAAAQAEASAYFMRALAYDEKRGRLDTTA
                     HPFTTTLGESDVRITTRYLEDYFPSSLFSTIHESGHALYEMGIDPAPEYRRTSLAEAS
                     SMAVHESQSRLWENTVGRSLGFWLQHLPALRQIVAPVLDGVGLEEFYRAINKVEPSLI
                     RTEADEVTYGLHVILRFELEGRMLSGDLAVDELPAAWNDGMRRLLGVTPSNDAEGCLQ
                     DIHWPMGSFGYFPSYALGNLYGAQWWDAMKAQGLDPKAAVELGDLGSILSWLRSNVHR
                     CGSMYRPGELVERVTGAPLDPSHFVRYLNDKYAGVYGL"
     gene            1285..2064
                     /locus_tag="PLW80_03865"
     CDS             1285..2064
                     /locus_tag="PLW80_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65671.1"
                     /translation="MGSDSATVAEALRMALATATFFVGLAGAAGLRSMLRHGDGGGTR
                     RANAAMAAASVAVAIAAWGFVTATRAAVAAPAGLSWYVAAGGVAGLLVGMFPRAAGVP
                     LALAAFASAALLSAGLSGWLPWEPGAEAASITVYSADSGGSSITLRYAVPGGGMVEQD
                     LSFGPGDLWLTFELVDIRGPASLAYGRRRYRLAGASDGGDGAIIKGDRGPLLDAAGGR
                     RLAGALGCSVAELEAPPFEPEPLAVVAYLLHDDGAVVMKER"
BASE COUNT          325 a          770 c          750 g          335 t
ORIGIN      
        1 cgggcccggg gagcctgcct accatagacc gcgcctacct gcgcgtgatg cgtcggaagt
       61 acgaccaggc gaccaagctg cctgccgagc tcgtctcgga gatggccagg gcgacgagcc
      121 tgtcccaggc ggcctgggtc gacgccagga agcataacga cttcaaagcc ttcgccccgc
      181 acctatccaa gatgctcgaa tataacaaga aggtggccgc ctgcctcgat ccgtccagga
      241 agccctacga cgtgctactc gaccgcttcg aggaaggcgc cagcgaggaa caggtcgcct
      301 cgatcttcgg gccgctacgc gaggagctcg tctccctgct cgataggata cgcggcaagc
      361 cgcagatcga cgactcgttc ctgcaccggc actgcccggc cgcggcccag gccgaggcca
      421 gcgcgtactt catgcgcgcg ctggcctacg acgagaagcg tggtcggctc gacaccacgg
      481 cccatccgtt cacgaccacg ctcggcgagt cggacgtacg catcaccacg cgctacctcg
      541 aggattactt cccgtcgagc ctgttcagca ccatccacga gagcgggcac gcgctatacg
      601 agatgggcat agacccggcg ccggagtacc gccgcacctc gctcgccgag gcttccagca
      661 tggccgtcca cgagtcgcag tcgcggctct gggaaaacac cgtcggccgc tcgctcggct
      721 tctggctaca gcacctgccg gctctcaggc agatcgtcgc acccgtcctc gacggcgtcg
      781 gactcgagga gttctaccgc gccatcaaca aggtcgagcc gtcgctgatt cgcaccgagg
      841 ccgacgaggt gacctacggc ctccacgtga tcctgcgctt cgagctcgaa ggacggatgc
      901 tatcgggcga cctagcggtc gacgagctgc cggcggcctg gaacgatggg atgcggcgcc
      961 tgctcggcgt cacgccgtcg aacgacgccg agggctgcct ccaggacata cactggccga
     1021 tgggctcctt cggctacttc ccgagctacg ccctgggcaa cctctatggc gcccagtggt
     1081 gggacgcgat gaaggcccag ggactcgacc cgaaggcggc cgtcgagctc ggcgacctcg
     1141 gttcgatcct atcctggctc aggtcgaacg tacaccgctg cggctcgatg tacaggcccg
     1201 gcgagctcgt ggagcgggtc accggagccc cgctcgaccc gtcgcacttc gtgcgctacc
     1261 tgaacgacaa gtacgctggg gtctatgggc tctgattccg caacggtcgc cgaagcgctg
     1321 cgcatggccc tcgcgacggc cacgttcttc gtcggattgg cgggtgcggc cggtttgcgc
     1381 tcgatgctga ggcacggcga cggcggcggt acgcgccggg ccaacgccgc gatggccgcc
     1441 gctagcgtgg ccgtcgccat cgcggcctgg ggcttcgtca cggcgacgag ggccgccgtc
     1501 gccgcgcccg ccggactctc ctggtacgta gccgctggcg gcgtagctgg cctcctcgtc
     1561 gggatgttcc cgagggcggc cggcgttccg ctcgccttgg ccgcgttcgc gtcggccgcc
     1621 ctgctgtcgg cgggactatc gggttggctg ccctgggagc cgggagcgga ggccgcctcg
     1681 atcacggtgt attcggccga ctcgggcggc tcgtcgataa cgctacgata cgccgttccc
     1741 ggcggcggga tggttgaaca ggacctgtcc tttgggccgg gcgacctctg gctgaccttc
     1801 gagctggtcg acatacgcgg ccccgcctcg ctcgcctacg gccggcggcg ctacaggctc
     1861 gccggggcgt cggacggcgg cgacggggcg atcatcaagg gcgaccgcgg gccgctcctc
     1921 gacgctgcgg gcgggcgacg gctggccggg gcgctcggct gctcggtggc cgagctggaa
     1981 gcgccgccct tcgagccgga gcccctggcc gtcgtcgcct acctgctgca cgacgacggc
     2041 gccgtcgtca tgaaagagcg ctagaggccc ggcgtcggcc gggccggagg cgctaggcgc
     2101 cgggtccgaa ggccacggcc aggcgttcca cggccgtctc gaccgtgcgc ctcgggcagc
     2161 cgaggttcat gcacgcgaag
//
LOCUS       DAOOLN010000236         2175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_63132_length_2175_cov_2.793396, whole genome shotgun sequence.
ACCESSION   DAOOLN010000236 DAOOLN010000000
VERSION     DAOOLN010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63132_length_2175_cov_2.793396"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="PLW80_03870"
     CDS             complement(<1..1181)
                     /locus_tag="PLW80_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HPB65672.1"
                     /translation="MKHRRLVAIALALTLALSSCGGNDRAVATAKRAKPTIGMVFDIG
                     GRDDASFNRSAYDGLAMLARDSGGRIEGDPDADFGDALDIRVLESKHGGVDREQLLRS
                     LAEDGRDLVIAVGFLFADSIGSVASDYPETHFVIIDGTVPDLNASSSVTCVDFAEHEG
                     SFLVGAYAGLLAAAAGPDARVGFVGGMDTPMIRRFLAGYTAGAAFINPALRAPGRVVS
                     AFVARDASGFDDPARGAEMASMLLRVNSKVIFHAAGASGRGVFEAAGRFDAKAIGVDS
                     DQAAILASSGSAADAQLSKLVVTSMLKRVDRAVYALGQELVAGVRVPGGYRTFGLAEG
                     GVGYVAAGLPADAVTTLAGGHVMIGGGHPSEVMPHIKAGRFKPLAVALPERDPSLPDV
                     PT"
     gene            complement(1240..1911)
                     /gene="deoC"
                     /locus_tag="PLW80_03875"
     CDS             complement(1240..1911)
                     /gene="deoC"
                     /locus_tag="PLW80_03875"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPB65673.1"
                     /translation="MSKEWTKATIAKTIDHTLLKAIATEQQVRELCVEAKANGFASVC
                     VNPRWVPLCAKELQNSKVLVCTVIGFPLGANATETKAAEAALAVKQGAEEVDMVINLG
                     AAKAGDWKEVEEDIRMVVKAAGKATVKVIIETCYLTDPEKVKACEAAARAGAHFVKTS
                     TGFGTGGASADDVRLMKKAVGDRLKVKASGGIRSYHDAIQMLDAGADRLGASSGVAIV
                     SELPE"
     gene            complement(1926..>2175)
                     /locus_tag="PLW80_03880"
     CDS             complement(1926..>2175)
                     /locus_tag="PLW80_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65674.1"
                     /translation="QEFLTRILEYGSPRALVLKSADRISNMISLGYTTDTAFVRRYCD
                     ETERFVFPIADKANKTMLAELVELVATRRDYLARCFEI"
BASE COUNT          333 a          729 c          746 g          367 t
ORIGIN      
        1 gtcggtacat ccggcagcga cgggtcgcgc tcgggcaggg ccacggccag cggcttgaag
       61 cgtccggcct tgatgtgggg catgacctcc gaagggtgcc cgccgccgat catcacgtgt
      121 ccgccggcga gcgtcgtcac ggcgtccgcc ggtaggccgg cggcgacgta gccgactccg
      181 ccctcggcga gtccgaaggt gcgatagcct cccgggaccc tgacgccggc gacgagctcc
      241 tggccgagcg cgtagacggc cctatcgacc cgcttgagca tcgaggtgac gacgagcttg
      301 gacaactggg cgtccgcggc tgagccggac gaggccagga tggccgcctg atccgaatcg
      361 acgccgatgg ccttggcgtc gaagcggccg gccgcctcga acacgccgcg gcccgaggcg
      421 ccggcggcgt ggaagatcac cttcgagttg acgcgcaaca gcatggaagc catctcggcg
      481 ccgcgggccg gatcgtcgaa gccgctcgcg tccctggcca cgaacgccga gacgacgcgg
      541 cctggagccc tgagggccgg gttgataaaa gcggccccgg ccgtgtagcc ggccaggaag
      601 cgccgtatca tcggcgtgtc catgcctccg acgaagccga cgcgggcgtc gggcccggcc
      661 gcggcggcca ggagaccggc gtaggccccg accaggaacg agccctcgtg ctcggcgaag
      721 tcgacgcagg tcacgctcga ggaggcgttc agatcgggca cggtgccgtc gatgatgacg
      781 aaatgcgttt caggataatc gctggcgacc gagccgatgg aatcggcgaa caggaagcct
      841 acggctatca ccaggtcccg gccgtcctcg gccaggctgc gcaggagctg ctcgcggtcg
      901 acgccgccat gctttgactc gagcacccgg atgtcgagcg cgtcgccgaa gtcggcgtcc
      961 gggtcgccct cgatgcggcc gccggagtcc cgggccagca tcgcgaggcc gtcgtaggcc
     1021 gaacggttga acgatgcgtc gtcgcggccg ccgatgtcga agaccatgcc tatcgtcggt
     1081 ttggctcgct tggcagtggc gacggcgcga tcgttaccgc cgcaggacga caatgccagg
     1141 gtcagggcca gcgcgatggc tacgagacgg cgatgcttca tggcgttcct ccgcggacag
     1201 tataacccgt ttcgggccgt agctggcgac taaagcgacc tactcgggca gctcgctgac
     1261 gatggccacg cccgacgacg cgccgagccg atcggcaccg gcgtcgagca tctggatggc
     1321 gtcgtggtag ctgcggatgc ctcccgaggc cttgaccttg agccggtcgc cgacggcctt
     1381 cttcatgagc cgcacgtcgt cggcgctggc cccgccggtg ccgaagccgg tgctcgtctt
     1441 gacgaagtgc gccccggccc tggccgccgc ctcgcaggcc ttgaccttct ccgggtcggt
     1501 caggtagcag gtctcgatga tcaccttgac cgtcgccttg ccggccgcct tgaccaccat
     1561 gcggatgtcc tcctcgacct ctttccagtc gcccgccttg gccgccccga ggttgatgac
     1621 catgtcgacc tcctcggcgc cttgcttgac ggcgagggcg gcctcggcgg ccttggtctc
     1681 ggtggcgttc gcgcccagcg gaaagcctat cacggtacag acgagcacct tcgagttctg
     1741 gagctccttg gcgcagagcg gcacccagcg cgggttgacg cagacgctgg cgaagccgtt
     1801 ggccttggcc tcgacgcaca gctcgcgtac ctgctgctcg gtggctatcg ccttcaagag
     1861 ggtgtgatcg atcgtcttgg cgatggtcgc cttggtccat tccttgctca tgtcgtctcc
     1921 ttggcttata tctcgaagca tcgcgccagg tagtcccggc gggtggcgac gagctcgacg
     1981 agctccgcca gcatggtttt attggcctta tcggctatcg ggaacacgaa acgctccgtc
     2041 tcgtcgcaat accgacgcac gaaggccgta tcggtggtgt agcccagcga tatcatgttg
     2101 ctgatcctgt cggcgctctt gagcacgagg gcacgcgggc tgccgtactc gaggatgcgc
     2161 gtcaggaatt cctgt
//
LOCUS       DAOOLN010000237         2170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_63318_length_2170_cov_3.128132, whole genome shotgun sequence.
ACCESSION   DAOOLN010000237 DAOOLN010000000
VERSION     DAOOLN010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63318_length_2170_cov_3.128132"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PLW80_03885"
     CDS             <1..194
                     /locus_tag="PLW80_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65675.1"
                     /translation="AAAEARDAERAAILADLDALDRRSARALRAVVAGTATDEDKAKL
                     AEIETEAVSKRSRLAGLEG"
     gene            199..474
                     /locus_tag="PLW80_03890"
     CDS             199..474
                     /locus_tag="PLW80_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65676.1"
                     /translation="MDYIAIGVSIFGVLGTLAGVLFALGKRDAKLDALEGRCREEWER
                     NSRQHAEFYMTRDTVTRIDTRFDDIARRMENMEDDVKEILSRLPKEA"
     gene            476..931
                     /locus_tag="PLW80_03895"
     CDS             476..931
                     /locus_tag="PLW80_03895"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPB65677.1"
                     /translation="MIHTDLSKLKPALFTRVTEALEEMRSDEKLKALGATGVAVSETL
                     REIAVQMAYYSRSRMAVMDVKAMYKAAGLYAIGDAEAKRAATWTLESRHLRGEAVDLV
                     PLKAGAYWWAAPEAVWKRMGEIGEAHGLEWGGRWKNADCPHFELRAGRI"
     gene            928..1158
                     /locus_tag="PLW80_03900"
     CDS             928..1158
                     /locus_tag="PLW80_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65678.1"
                     /translation="MMVGPFQEADGAVSMRRILALFFALAAVALGVAAIPGAPGWYVF
                     IPTILCVGACLLLLFFTTWADVATIVASWKGH"
     gene            1143..1661
                     /locus_tag="PLW80_03905"
     CDS             1143..1661
                     /locus_tag="PLW80_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65679.1"
                     /translation="MEGPLMCRDDQSARWWLLALLGLVLLFAARTVSGQELLTSDTPT
                     PSPQSNETLPNTDPTPTDQWASFDQLWTSLKDELTQSDADSQALLISLEALRIEADEL
                     RSSLTESTRLYEQSEASRMIEREAAETRITDAILRGIDAEKSRDRWKITAIILGGVGI
                     ILAGSLALSIVL"
BASE COUNT          429 a          637 c          689 g          415 t
ORIGIN      
        1 aggcggcggc agaggcccgc gacgccgagc gcgccgcgat actcgcggat ctggacgcgc
       61 tcgaccgccg ttcagcccgt gcgctccggg ccgtggtcgc ggggacggct accgacgagg
      121 acaaggccaa gctggccgaa atagagaccg aggcggtgag caagcggtcg aggcttgctg
      181 gactggaggg atagcggtat ggactatata gcgatcggag tttcgatctt cggcgttctc
      241 ggcaccctcg ccggcgtgct cttcgccctc ggcaagcgag acgcgaagct cgacgccctc
      301 gagggccggt gccgcgagga gtgggagcgc aactcccgcc agcacgcgga attctacatg
      361 acccgcgata ccgtgacgcg gatcgatacc aggttcgacg acatcgctcg tcgtatggaa
      421 aacatggagg acgacgtcaa ggaaatcctc tcgcggctcc cgaaggaggc ataggatgat
      481 ccataccgac ctctcgaagc tcaagcccgc gctcttcacc cgcgtgaccg aggcgctcga
      541 ggagatgcgt tccgacgaga agctgaaggc tctcggagcg accggggtcg ccgtgtcgga
      601 gacgctccgc gagatcgccg tgcagatggc ctactattcg cgctcccgca tggccgtgat
      661 ggacgtcaag gccatgtaca aggcggccgg cctgtacgcg atcggcgacg ccgaggcgaa
      721 gcgcgccgcg acctggacgc tagagtcacg acacctccgc ggggaggccg tcgacctcgt
      781 gccgctcaag gccggcgcct actggtgggc ggcgcccgag gccgtgtgga agcgcatggg
      841 cgagatcggc gaggcgcacg gtctcgagtg gggcggccgc tggaagaacg cggactgccc
      901 gcactttgag cttcgggctg ggaggatatg atggtcggcc cgttccagga agcggacggc
      961 gcggtctcga tgcgccggat cctcgcgctg ttcttcgcgc tcgcggcggt cgccctcggg
     1021 gtggccgcga taccgggcgc gccggggtgg tatgttttta tcccgaccat actgtgcgtc
     1081 ggggcttgcc tgctgctcct cttcttcacg acctgggccg acgtggcgac catcgtcgcg
     1141 tcatggaagg gccactgatg tgtcgcgacg atcaatctgc gcgctggtgg ctactggcgc
     1201 tcttgggctt ggtcttgctc tttgcggcgc gtaccgtctc ggggcaagag ttgctgacca
     1261 gcgacacgcc gacgccatcg ccgcaatcga acgagacttt gccgaatacc gatccgacgc
     1321 cgacagatca atgggcgagc ttcgatcagc tctggacgag tctcaaggac gagttgactc
     1381 aatcggacgc ggactcgcaa gcgctattga tctcgctaga ggcgttgcgg atcgaggccg
     1441 acgaattgcg atcctcattg acggaatcga cgaggctcta cgagcaatcc gaggcgagcc
     1501 ggatgattga gcgcgaggcc gcagagacaa gaataaccga tgcgatactt cgcggaattg
     1561 acgccgaaaa atcacgcgac cgatggaaaa tcactgctat tattctcgga ggtgtgggga
     1621 ttatactcgc tggtagcctt gcgctttcaa tagtgctata atcgattaaa aggtggcgaa
     1681 tagtgcctag aacaatcgag caaagcctta gtataaaatc actggacgac gatatcgggg
     1741 ttgacaacac agccgagcgg ctaggtatta caagggagtc ggtaaggaga tcgatccgcg
     1801 ttgccagaaa agagattagg caagatccgg tcgccccggg cgacttgcta gactagatta
     1861 aatgcccaca atatacataa caatcgatag caatgtatgt tctccggcta cttggtaaaa
     1921 ctagccgcgc cgcgttcagc gcgcttcctg tcggatcttt ccctatatcc gcgatccgcc
     1981 gatatttggt gccggatagc ttcgagaacg tagctccgct tgtccattcc gggcttgcgc
     2041 gatatctcat ctagcatcgc agtctctagt tcttcgggta gtttaatctc catctatcgc
     2101 ctcctctagc ctatctatcg cggcctcgat cctagctgcc tttaccgcct cgactatgcg
     2161 cgagcactcg
//
LOCUS       DAOOLN010000238         2167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_63465_length_2167_cov_2.805871, whole genome shotgun sequence.
ACCESSION   DAOOLN010000238 DAOOLN010000000
VERSION     DAOOLN010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63465_length_2167_cov_2.805871"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..745
                     /locus_tag="PLW80_03910"
     CDS             2..745
                     /locus_tag="PLW80_03910"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HPB65680.1"
                     /translation="MGEGFGAYSIGDDEGVLRYVTSANIACGFHAGDPSVMERTVALA
                     ASASVSIGAHPGFPDLLGFGRRELSASADEARAMTLYQIGALGAFAKARGVALTHVKP
                     HGALYNMAAKRADLAAAIAEAVAAYDPALILVGLCGSELLKAGTAAGLRCAGEAFADR
                     AYRADGSLVPRGIPGAVIKDPASCAARVISMATDGFVTALSGERVMLRPDTICLHGDG
                     DGALELAEALSTALREAGVELWPLSHTDA"
     gene            786..>2167
                     /gene="pxpB"
                     /locus_tag="PLW80_03915"
     CDS             786..>2167
                     /gene="pxpB"
                     /locus_tag="PLW80_03915"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HPB65681.1"
                     /translation="MLVRLGDVLSEELNERARSLADAVEAAKADDPADDGIIEASPAY
                     TSVFIDFDPLLLTHAQAEAFVRASLSAVSSGEARRVVRVPVAYGGEAGPDLASIAAEA
                     GLSADEVASIHCSRDYPVYMLGFAPGFPYLGGVDERIASGRLKTPRPRIPAGSVGIAG
                     RQTGVYPFDSPGGWKLVGRSPICLFDASMDPPATLRPGDLVRFYPVSLDEFDAMSRGP
                     GGIAEHREGPSGPTDPLERAAIRVVRPGLSTTVQDLGRRGYQKYGVPVSGAMDNRSAS
                     LANRLVGNAPTDACLETTLIGPSLEFLADVEFVVTGAPSAMTLDGAPVPMYERVRARR
                     GSVLEMPPVSRGARSYLAVAGGFAIDPVMGSRSTYARAGLGGLRGRRLQTGDLLPVGP
                     APARLADGDGRREPDSSGSPALQPFASGEPARIRVVSGPEADALGPDCLAAFTTREYR
                     VSADSDRMGY"
BASE COUNT          312 a          790 c          722 g          343 t
ORIGIN      
        1 cctgggggaa ggcttcggcg cctactccat cggggacgac gagggcgtgc ttcggtacgt
       61 gacctcggcc aatatcgcct gcggcttcca tgccggcgac ccgtcggtca tggagcgtac
      121 ggtcgcgctg gccgcgtcgg cgagcgtttc gatcggcgca cacccgggct tcccggatct
      181 cctgggtttc ggacggcgcg agctatcggc gagcgccgac gaggcgcgcg cgatgacgct
      241 ctaccagata ggcgccctcg gggccttcgc caaggctcga ggcgtcgcgc tgacgcatgt
      301 caaaccacac ggcgcgctgt acaacatggc ggccaagcgc gctgacctcg ccgccgccat
      361 agccgaagcg gtggccgcct acgatccggc gctgatcctc gtcggcctct gcggctcgga
      421 gctcctcaag gccggaaccg ccgcgggcct ccgttgcgcc ggcgaggcct tcgccgaccg
      481 agcctatcgt gccgacggtt cactcgtacc ccgcggtatc ccgggcgccg tcatcaagga
      541 tccggcctcc tgcgccgcga gggtcatcag catggccacc gatggcttcg tgaccgcctt
      601 gagcggcgag cgggtcatgc tgcgccccga cacgatctgc ctccacggcg acggcgacgg
      661 ggcgctcgag ctggccgagg ccctatccac ggccctcagg gaggccggcg tcgagctctg
      721 gcccctttcg cacaccgatg cctaacctct ctcagtttga atgcatccct ctcggcgatg
      781 atggattgct cgtcaggctc ggggacgtgc tgtccgagga gctgaacgag cgggcgcgtt
      841 cgctcgcgga cgccgtagag gccgccaagg ccgacgaccc ggccgacgac ggcatcatcg
      901 aggcgtctcc cgcctatacg tcggtgttca tcgacttcga cccgctcctc ctgacccacg
      961 cgcaggccga ggccttcgtc cgggcgtcgt tgtccgccgt ctcaagcggc gaggctcgaa
     1021 gggtcgtccg ggtacctgtg gcctacggcg gcgaagccgg ccctgacctg gcctcgatag
     1081 ccgctgaagc gggcctatcg gccgacgagg tcgcgtccat ccactgcagc agggactatc
     1141 cggtgtacat gctgggcttc gcgccaggct tcccctacct cggaggggtc gacgagcgca
     1201 tagcctctgg ccgtctcaag acgccccgcc ccagaatacc ggcgggatcc gtcggaatag
     1261 ccgggcgtca gaccggggtc tatcccttcg acagcccggg cgggtggaaa ctcgtcggac
     1321 gtagcccgat atgcctcttc gacgcgtcga tggacccgcc ggccacctta agaccgggcg
     1381 acttggtacg cttctatccc gtcagcctcg acgagttcga cgccatgagc cgcggacctg
     1441 gcggcatagc cgagcatcgc gaaggtccga gcggcccgac cgaccctctt gagcgcgcgg
     1501 ccatacgcgt cgtcaggccg ggtctctcga ccaccgtgca agacctcggt cgacgcgggt
     1561 accagaaata cggcgtaccc gtctcgggtg ccatggacaa ccggtcggct agcctggcca
     1621 accggctcgt cggcaacgca ccgacggacg cctgcctcga gacgaccctg atcggtccat
     1681 cactcgaatt cctggccgac gtcgagttcg tggtaaccgg ggcgccatcc gccatgacgc
     1741 tcgacggcgc gccggtaccg atgtacgaac gcgtccgcgc aaggcgcggc tcggtactcg
     1801 agatgcctcc ggtctcgcga ggcgcgcggt cgtacctcgc cgtggccgga ggcttcgcga
     1861 tcgatccggt tatgggctcg cgttccacct acgcgcgcgc cgggctcggc gggctacgcg
     1921 gccgccgcct ccagacggga gacctgctcc cggtcggacc cgcgccggcg cgcctcgcgg
     1981 atggcgatgg gcgtcgggaa ccggacagct ccggctcacc ggctctccag ccgttcgcgt
     2041 ccggagagcc ggcgcggatc cgcgtcgtct cggggcccga agccgacgcc ctcggccccg
     2101 attgcctcgc cgccttcacg acgcgcgagt atcgcgtgtc cgccgactcc gaccgcatgg
     2161 gctacag
//
LOCUS       DAOOLN010000239         2160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_63763_length_2160_cov_3.914489, whole genome shotgun sequence.
ACCESSION   DAOOLN010000239 DAOOLN010000000
VERSION     DAOOLN010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63763_length_2160_cov_3.914489"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1679
                     /locus_tag="PLW80_03920"
     CDS             <1..1679
                     /locus_tag="PLW80_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HPB65682.1"
                     /translation="LLAAATTVYVIVDYIGIVGRAGMPNLTDLVFAALLILLVLEGAR
                     RVTGWALPALGVVFILYALFGRGLPGFFGHRGYSWADLSSFLFVSTDGVYGTAVGVSA
                     SYIYLFILFGAFMDKSGMGQFINDIALALAGHQKGGPAKVSVIASALLGTINGSAVAN
                     VVTTGTFTIPLMKKTGYDKDFAGAVEASSSVGGQLMPPIMGAAAFIMAEVLGIQYKVI
                     AVAAILPALLYYVGIIVQVHPRARKCGLEGVSRENLPKAWAVLKERGHLLLPIVFLLV
                     MLFASGKTVIFSAFMTILVTIATSMLKKSTRMSLKDILDALFEGTRSMVPVAIACASV
                     GLVIGVASLTGFGLSMASAIVALGGKSLFLTLAFTMVACMILGMGLPSIPAYLITATM
                     AAPALVRLGIDPLVSHLFVFYFAMFANITPPVALASFAAAGLSGGSPMKTGFTSLKLS
                     LAGFIVPYMFVYNDALLLQGTGFFEGFLVMMTSISGVLILGTAIEGYFISKMGVVERI
                     IALAAAILLMTPNHLQDLVGAGLAVVVIAVQLIKRKRSIEAACEDTVAKA"
     gene            1812..>2160
                     /locus_tag="PLW80_03925"
     CDS             1812..>2160
                     /locus_tag="PLW80_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HPB65683.1"
                     /translation="MLEYVVAENPDARVMRRAASVLQDGGLVVVPTDTSWSVLCSIAS
                     KGGIAALKRLIEPGRERPLTAMCSSIAQASELCDVSSAAFRVMKRLTPGPYVFILPST
                     NRSMRSLDLRRAEL"
BASE COUNT          323 a          752 c          662 g          423 t
ORIGIN      
        1 cgctgctcgc cgcggcgacc acggtctacg tgatagtcga ctatatcggc atcgtcggcc
       61 gcgccggtat gcccaacctg accgacctcg tcttcgccgc cctgctgata ctgctcgtgc
      121 tcgagggcgc caggcgcgtg accggctggg ccctgccggc gctcggcgtg gtcttcatcc
      181 tgtacgcctt gttcggtcgc ggcctgcccg gcttcttcgg ccaccgcggt tattcatggg
      241 ccgacctgtc gagcttcctg ttcgtctcga ccgacggcgt ctacggcacg gccgtcggcg
      301 tgtcggcctc gtacatctac ctgttcatcc tgttcggcgc cttcatggac aagtccggca
      361 tggggcagtt catcaacgac atcgccttgg ccctggccgg ccatcagaag ggcgggccgg
      421 ccaaggtgtc ggtcatcgcg agcgccctcc tcggtacgat caacggctcg gcggtagcga
      481 acgtcgtcac cacgggcacc ttcacgatcc cgctcatgaa gaagaccggc tatgacaagg
      541 acttcgccgg cgcggtcgag gcctcgtcct cggtcggagg ccagctgatg cccccgatca
      601 tgggcgcggc cgccttcatc atggcggagg tgctcggcat ccaatacaag gtcatagccg
      661 tcgcggccat cctgccggcc ctcctgtact atgtcggcat catcgtgcag gtgcacccgc
      721 gggcccggaa gtgcgggctc gaaggcgtat cgcgcgagaa cctgccgaag gcctgggccg
      781 tgctcaagga gcgcggccat ctgctcctgc ccatcgtgtt cctgctggtc atgctcttcg
      841 cctccggcaa gacggttatc ttctcggcct tcatgaccat actcgtcacc atcgcgacgt
      901 cgatgctcaa gaagtccacc agaatgagcc tcaaggacat cctcgacgcg ctgttcgagg
      961 gtaccaggtc gatggttccc gtagcgatag cctgcgcctc cgtcggcctc gttatcggcg
     1021 tggcgagcct gaccggcttc ggcttgtcga tggccagcgc catcgtcgcc ctcggcggca
     1081 agagcctgtt cctgacgctc gccttcacga tggtcgcctg catgatcctg ggcatgggcc
     1141 tgccgagcat acccgcctac ctgatcaccg ctacgatggc cgccccggcc ctcgtgcgac
     1201 tcggcatcga cccgctcgtg tcgcacctgt tcgtcttcta cttcgctatg ttcgccaaca
     1261 tcacgccgcc ggtggcgctc gcttcgttcg ccgccgcggg actgtcgggc ggcagcccga
     1321 tgaagacagg tttcacctcg ctcaagctgt cgctcgccgg cttcatcgtg ccatacatgt
     1381 tcgtgtacaa cgacgcgctg ctcctacaag gtacggggtt cttcgaggga ttccttgtca
     1441 tgatgacctc gatcagcggc gtgctcatcc tcggcacggc gatcgagggt tacttcatat
     1501 cgaagatggg cgtcgtcgag cgaataatcg cgctcgccgc ggccatcctg ctcatgacgc
     1561 cgaatcatct ccaggatctc gtcggcgccg ggctggcggt cgtagtcata gcggtacagc
     1621 tgatcaagcg caagcgctcg atcgaggcgg cctgcgagga tacggtggcc aaggcctagg
     1681 gccggctcca ggcttacgct ccgccagcgc cgcgatgata ccgctcgaag cggtcgctgt
     1741 cggagctgac ggtacccctg gcacgttgcg tccgaggcgc aaagggagta gtatcgtctc
     1801 ggaggccatc cttgctcgag tacgtcgtcg ccgagaatcc tgacgcccgc gtcatgcgcc
     1861 gcgccgcctc cgtcctgcag gacggaggcc tcgtcgtcgt gccgaccgac accagctgga
     1921 gcgtcctgtg ctccatcgcc tccaagggcg gcatcgccgc cctcaagcgc ctgatcgagc
     1981 cgggccgaga gcgcccgctg accgcgatgt gctcgtcgat cgcccaggcc tccgagctgt
     2041 gcgacgtgtc gagcgccgcc ttcagggtca tgaagcgctt gacgcccggc ccttacgtat
     2101 tcatcctgcc gagcaccaat cgctcgatgc gcagcctcga cctcaggcgg gccgagctag
//
LOCUS       DAOOLN010000240         2160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_63767_length_2160_cov_3.665083, whole genome shotgun sequence.
ACCESSION   DAOOLN010000240 DAOOLN010000000
VERSION     DAOOLN010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63767_length_2160_cov_3.665083"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PLW80_03930"
     CDS             complement(<1..793)
                     /locus_tag="PLW80_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPB65684.1"
                     /translation="MPRFVSSSRYPIARSISLAAFVAAAFVSSTAATPVEVVGLLSRA
                     ETIADSLDDEGLVGQLMMIGVEGVGRPSNASRLILERIRPGAVILFGFNVDDDPRSVA
                     SLAGAVRDACSVDTARIRSLPPFVAIDHEGGGVYRFKGGLTRLPAAATMGLAGAAAAS
                     AAGSAAGTELRALGVTMNAAPVVEALNEANRGFLAGRAWSDDPAEAAKLSASFIVSCQ
                     RGGAAAVAKHFPGNAEVDPHRGLPVLTASRAVVEGAYLAPFRAAVN"
     gene            complement(843..1841)
                     /locus_tag="PLW80_03935"
     CDS             complement(843..1841)
                     /locus_tag="PLW80_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HPB65685.1"
                     /translation="MGVLIPIGSGKGGVGKTVFTANLGAVLASYGKTVILVDLDLGGA
                     NLHTCLGVRNNHPGVGSIIWKKEKRLENLVVPTGVDRLFLVPGDNLLPGTANLEFFTK
                     RRIMKELLELTADYVLVDLGAGASYNVIDFWLMSPDGIVVTAPEIPAILNAYSFLKTA
                     AFRLLFRSFKKGSEERERIVDFVVNRSEGQGDTFLAFAERLAASDGAAGSAAGLKALE
                     ELRRLRPRVVVNQGSGESDAAVAQRLREISSRNLGLGMEYIAYIMRDQNVPASIAGRS
                     PLAVSDPDAMFIRGVKAAADRILRAPEAPAPGLELDDEDLFSMIERSFDEQGSPAL"
     gene            1968..>2160
                     /locus_tag="PLW80_03940"
     CDS             1968..>2160
                     /locus_tag="PLW80_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65686.1"
                     /translation="MRRYAIAACAILSAAVAAAFSWTSTPTIHVEAASLNGLASLSVR
                     CEVRGAEVWLDYQYRGTTPL"
BASE COUNT          357 a          711 c          760 g          332 t
ORIGIN      
        1 cgttcacggc cgccctgaac ggcgccaggt aggcgccctc gaccacggcg cgggacgccg
       61 tgagcaccgg caggccgcga tgcgggtcga cctcggcgtt gccgggaaag tgcttggcca
      121 ccgcggccgc accgccacgc tggcaggaca cgatgaagga ggccgacaac ttggccgcct
      181 cggccgggtc gtccgaccag gccctgccgg cgaggaagcc ccgattggcc tcgttgagtg
      241 cctcgacgac cggagccgcg ttcatcgtga cgccgagggc tcggagctcc gttcccgcgg
      301 cgctcccggc ggccgaagcg gccgccgcgc cggccagacc catcgtcgcg gcggccggaa
      361 gccgtgtcag gccgcccttg aagcgataga cgccgccgcc ctcgtggtcg atggcgacga
      421 agggcggcag ggagcggatc cgggccgtgt cgacggagca ggcgtcgcgg accgcccccg
      481 cgagcgaggc taccgaccgg ggatcgtcgt cgacgttgaa cccgaagagt atgaccgcgc
      541 caggcctgat acgctcgagg atgaggcggc tggcgttcga tggccgaccg acgccctcga
      601 cgcctatcat catgagctgg ccgaccagcc cctcgtcatc gaggctatcg gcgatagtct
      661 cggctcggga gaggagcccg acgacctcga cgggcgttgc ggccgtcgag gacacgaagg
      721 cggccgcgac gaacgcggcc aggcttatgg aacgggcgat aggataacgg gaggacgata
      781 cgaaccgagg catggggcga tgatagtcgc cgaggaccct agcggcaagg gccgccccgg
      841 gatcagagcg ccgggctacc ctgttcgtcg aacgagcgct ctatcatcga gaataggtcc
      901 tcatcgtcga gctcgagccc cggggcgggg gcctcgggcg ctcgcagtat cctatcggcg
      961 gcggccttga cgccgcggat gaacatggcg tcggggtcag agacggccag gggcgatcgg
     1021 ccggcgatgg aggccggcac gttctggtcg cgcatgatat aggcgatata ctccatgccc
     1081 aggccgaggt tgcgcgacga tatctcgcgg aggcgctggg cgacggccgc gtcactctcg
     1141 ccgctgccct gattgacgac gacgcgcggc ctcaggcgtc gtagctcctc gagcgccttg
     1201 agcccggcgg cgctacccgc ggcgccgtcg gacgccgcta gccgctcggc gaaggccagg
     1261 aaggtatcgc cctggccctc cgaccggttc acgacgaagt cgacgatgcg ctctcgttcc
     1321 tcgctgccct tcttgaacga tcggaacagc aacctgaacg cggcggtctt caggaaggag
     1381 taggcgttca ggatagcggg gatctcaggc gccgtgacga cgatgccgtc aggggacatg
     1441 agccagaagt ctatgacgtt atacgaggcg ccggcgccca ggtcgacgag cacgtagtcg
     1501 gcggtcagct cgagcagttc cttcatgatg cggcgcttgg tgaagaactc gagattggcc
     1561 gtaccgggta gcaggttgtc gccaggcacg aggaacaggc ggtcgacgcc ggtcggcacg
     1621 accaggttct cgaggcgctt ctccttcttc cagatgatcg agccgacgcc cgggtggttg
     1681 ttacgtaccc cgaggcaggt atgcaggttc gcccctccca ggtccaggtc gacgagtatg
     1741 acggtcttgc catacgaggc gagcacggcg ccgaggttgg ccgtgaacac cgtcttgcct
     1801 accccgccct tgcccgagcc tatcggaatc agtacgccca cgtcggtctc ctgcgcgttc
     1861 gagtatcgtt cgattgtaag ccggaatggc cggggccaca agcgcggatc gcgtccgggc
     1921 ggttcggacg cttgtggtcg ccgacgcggc gcggtacagt atacgacatg agacgatacg
     1981 ccatcgccgc ctgcgcgatc ctttcggccg ccgtggccgc agccttctcc tggacctcga
     2041 cgccgaccat ccatgtggaa gccgcttcgt tgaacgggct cgcctcgcta tcggtgcgtt
     2101 gcgaggtacg cggcgcggag gtctggctcg actaccaata tcgcggcacc acgccgctcg
//
LOCUS       DAOOLN010000241         2160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_63772_length_2160_cov_3.402850, whole genome shotgun sequence.
ACCESSION   DAOOLN010000241 DAOOLN010000000
VERSION     DAOOLN010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63772_length_2160_cov_3.402850"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLW80_03945"
     CDS             <1..334
                     /locus_tag="PLW80_03945"
                     /inference="COORDINATES: protein motif:HMM:NF015164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HPB65687.1"
                     /translation="VLAPVAGGSPRAAFDSFARWRLSHESPNAGHPEAAYAGALGLRL
                     GGPSRYAEGIIDKPWINPTGAEATSAHVSRAVRLMYAQVLLSATAFLSLRIALGTLLS
                     FALAAIVA"
     gene            635..1696
                     /locus_tag="PLW80_03950"
     CDS             635..1696
                     /locus_tag="PLW80_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HPB65688.1"
                     /translation="MNKFVKRYLVDALGGMALGLFSTLIIGLILKQVGGFFPGTAPGR
                     FLVGLGNLATVLTGAGIAAGVAHSLGSPRLVIYASLLCGLVGAYASRFVDGSLFSAAG
                     VALSGPGDPLGALVAALAGAELGRLVSGKTRVDIIVTPAVTIVAGCLAGALLGPTLSQ
                     GSIALGGIIRYATELRPFLMGVVISVCMGLFLTLPISSAAIGIILGLSGLSAGAATAG
                     CSAQMIGFAVISFRDNGWNGLFAQGLGTSMLQMPNIVRNWRIWIPATAASAITGPLAT
                     TVFGMMNLPAGSGMGTSGLVGPLLAWQAMTGAGMGAPRVLFSILMVCLVLPALLSYAF
                     YALMRKKGWIRDGDMKLSS"
     gene            1866..>2160
                     /locus_tag="PLW80_03955"
     CDS             1866..>2160
                     /locus_tag="PLW80_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65689.1"
                     /translation="MPATKAPSANKRLIEGLVTSVVLIACFGYLGSVMGAKNLINTIT
                     LTAHDLLLNTVFYLMAITVIVGALAAILTEFGVVRIIERLMAPFMKPLFGLPGV"
BASE COUNT          291 a          788 c          680 g          401 t
ORIGIN      
        1 cgttctcgcc ccggtagccg gcgggtcgcc acgcgcggcg ttcgactcgt tcgcgcgctg
       61 gcggctgtcc cacgaaagcc cgaacgccgg ccaccccgag gcggcctacg ccggcgccct
      121 cggcctcagg ctcggcggac cgtcacgcta cgccgaggga atcatcgaca agccctggat
      181 caacccgacc ggcgccgagg ccacttcggc ccacgtcagc cgggccgtca ggctcatgta
      241 cgcgcaggtc ctgctatcgg cgaccgcctt cctttccctg cggatcgccc tcgggacact
      301 cctgagcttc gccctagcgg ccatcgtcgc ctaagcgagg ccgagatatg ggtgctaggc
      361 tgctatcctc ccatcgcccg cttgacgcgt gttcgcccgc ttgacgcgtg ttcgcccgct
      421 tgacacgtgc tcgcccgctt gacgcgtgtt cgcccgcttg acgcgtgttc gcccgcttga
      481 cgcgggcggc ggggacgggt atggtcggca tggtaccgga gtctcctgga gcaaaccaac
      541 gctccctgac ggagctatgt gcgtgtcccg tccgaaagat ccggcccgtc tcccgcctgg
      601 gcatggatcg gtaccgcatg gagcgctacg cgccatgaac aaattcgtca agcgttacct
      661 cgtggacgcg ctcggtggca tggccttggg cctgttctcc accctgatca tcggcctgat
      721 actcaaacag gtcggcggct ttttcccggg caccgcgccg ggtcgcttcc tcgtcggcct
      781 cggcaaccta gccacggtgc tgaccggcgc gggcatcgcg gccggcgtgg cccactcgct
      841 cggcagcccg aggctcgtga tctacgccag cctcctctgc ggcctcgtcg gcgcctacgc
      901 ttcgcgcttc gtggacggct cgctattttc ggcggccggc gtggccctgt ccggccccgg
      961 agacccgctc ggcgccctcg tcgcggcgct cgccggcgcg gaactcggtc gcctcgtctc
     1021 gggcaagacc cgcgtcgaca tcatcgtcac gcccgccgtg accatcgtcg ccggctgcct
     1081 ggccggcgcc ctgctcggcc cgacgctgtc ccagggctcc atcgcgctcg gtggcatcat
     1141 ccgctacgcc accgagctca ggcccttcct gatgggcgtc gtcatctcgg tctgcatggg
     1201 cctcttcctg accctgccga tcagctcggc cgccatcggc atcatcctcg ggctgtcggg
     1261 gctgtcagcg ggggccgcca ccgccggctg ctcggcccag atgataggct tcgccgtgat
     1321 cagcttcagg gacaatggct ggaacggtct gttcgcccag ggcctcggaa cctcgatgct
     1381 gcagatgccg aacatcgtgc gcaactggag gatctggata cccgccacag ccgcgagcgc
     1441 gatcaccggg ccgctcgcca ccacggtctt cggcatgatg aatctgccgg ccggctcggg
     1501 catgggcacc tcgggtctcg tcggcccgct cctggcctgg caggcgatga ccggggccgg
     1561 tatgggcgct ccgcgcgtcc tgttctcgat cctgatggtc tgcctcgtgc tcccggccct
     1621 gctgtcgtac gccttctacg cgctgatgcg caagaagggc tggatacgcg acggcgacat
     1681 gaaactgagt tcctagggcg acgatagcgg cggccctagg cgtcagccgg ccgccgctat
     1741 cggctccgcg gcccttggga tgggacccgg gcgcgagcgt tcttttctat cgataccgac
     1801 gtagcgattg acgcgcgtct cgccttccga ggatactcgc tccgcgatca cttacggagg
     1861 aaatcatgcc agccaccaag gccccgtccg cgaacaagcg gctcatcgag ggcctcgtca
     1921 cgtccgtcgt ccttatcgcc tgcttcggct acctgggctc agtcatgggg gccaagaacc
     1981 tgatcaacac gatcacgctg acggcccacg acctgctcct gaacactgta ttctacctga
     2041 tggcgatcac cgtgatcgtc ggcgcgctag ctgccatcct gaccgagttc ggcgtcgtgc
     2101 gcatcataga gcgcctgatg gcgccgttca tgaagccgtt gttcggcctg cccggcgtgg
//
LOCUS       DAOOLN010000242         2152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_64127_length_2152_cov_3.212208, whole genome shotgun sequence.
ACCESSION   DAOOLN010000242 DAOOLN010000000
VERSION     DAOOLN010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64127_length_2152_cov_3.212208"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..1886)
                     /gene="glmS"
                     /locus_tag="PLW80_03960"
     CDS             complement(42..1886)
                     /gene="glmS"
                     /locus_tag="PLW80_03960"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPB65690.1"
                     /translation="MCGIVGYTGPRQASRILVEGLKRLEYRGYDSAGIAVRRDEPLKD
                     GEAPLSVIKKTGKIAELRQAVPKDLPGTCGIGHTRWATHGGVTDANAHPHVDGSGKIA
                     IVHNGIIENSGSLKEMLEKEGAVFASETDSEVIAHLVAYYYDGDLETALKAALGHLRG
                     TYGIAAIHADEPGRIVGARNGSPLVVGVGDGEMLLASDVTALLAHTASVVYMNDGEVV
                     SMTPAEFRISDGDDRAVDPKIDTITWKLDAIEKEGFSSYMEKEIFEQPESIDRALTGR
                     MDLEYGSAKLGGLNLSKKELHAIERVRVIAAGTSWHAGLVGCQLLESVARLPAQAELA
                     SELRYRNPVVEPDSLYLAVSQSGETADTLYAMREIQRRGGSVLGVCNVVGSTIARESD
                     GGIYVHSGPEIAVASTKAFTSQLAAFYLLTLSLARLHDMSHHEGQRFLAELRAIPEAV
                     RACLERRDAIQAIAKRYARYRDFLFLGRGLLYPIALEGALKLKEISYIHAEGYASGEI
                     KHGPIALVSPETPSVFLVSNDHLREKTISNIREIKARGGPVIALAQEGDDEVAGLADE
                     FIPVPAVGSRFQPFVMVPPLQLLSYFCALELGRNVDQPRNLAKSVTVE"
BASE COUNT          308 a          720 c          756 g          368 t
ORIGIN      
        1 cgccaaggcc cgagcggcgg gacgaacgac ggggccgggc atcattccac cgtcacgctc
       61 ttggccaggt tgcgcggctg gtcgacgttg cgcccgagct cgagcgcgca gaagtacgac
      121 aggagctgca gcggcggcac catcacgaat ggctggaagc gagagccgac cgccggcacc
      181 gggatgaact cgtcggccag gccggccacc tcgtcgtcgc cttcctgggc cagcgctatc
      241 accgggcccc cgcgcgcctt gatctcgcgg atgttcgaga tggtcttctc gcgcaggtgg
      301 tcgttggaca cgaggaacac gctcggcgtc tccgggctga cgagggctat cggcccgtgt
      361 ttgatctcgc ccgaggcgta gccctccgcg tggatgtacg aaatctcctt gagcttgagc
      421 gcgccctcga gggctatcgg gtacaggagg ccacgcccga ggaacaggaa gtcgcggtag
      481 cgggcgtaac gcttggcgat ggcctggatc gcgtcgcgcc gctcgaggca ggcgcgcacc
      541 gcctcgggta tagcgcgcag ctccgccagg aagcgctgcc cctcgtggtg cgacatgtcg
      601 tgcaggcggg ccagcgacag ggtcaggagg tagaaggcgg cgagctggct cgtgaaggcc
      661 ttggtcgacg cgacggctat ctccgggccg gagtgcacgt agatgccgcc gtcgctctcg
      721 cgggctatgg tcgagccgac gacgttgcag acgccgagca ccgagccgcc gcggcgctgg
      781 atctcgcgca tcgcgtagag cgtgtcggca gtctcgccgg actgcgagac cgcgaggtac
      841 agggagtcgg gctcgaccac cgggttgcgg tagcgcagct cgctggccag ctcggcctgg
      901 gccggcaagc gcgccaccga ctccaggagc tggcagccga cgaggccggc gtgccagctc
      961 gtgccggccg ctatgacgcg cacgcgctcg atcgcgtgca gctccttctt ggacagattg
     1021 aggccgccga gcttggccga gccgtactcc aggtccatgc ggccggtcag ggcgcggtct
     1081 atggactctg gctgctcgaa gatctccttc tccatgtacg acgagaagcc ttccttctcg
     1141 atcgcgtcga gcttccaggt gatcgtgtct atcttcgggt cgacggcgcg gtcgtcgccg
     1201 tcggatatcc tgaactcggc cggcgtcatc gacaccacct cgccgtcgtt catgtagacg
     1261 acgctcgcgg tatgggccag gagcgcggtc acgtcgctcg cgagcagcat ctcgccgtcg
     1321 cctacgccga cgacgagggg gctgccgttg cgggccccga cgatgcggcc gggctcgtcg
     1381 gcgtggatgg cggctatgcc gtaggttccg cgcaggtgtc cgagggccgc cttgagcgcc
     1441 gtctccaggt cgccgtcgta gtagtaggcg acgaggtggg cgatcacctc gctgtcggtc
     1501 tcgctcgcga aaacagcgcc ttccttctcg agcatctcct tgagggagcc cgagttctcg
     1561 atgatgccgt tatggacgat ggcgatcttg cccgagccgt cgacgtgcgg atgggcgttg
     1621 gcgtcggtca ccccgccgtg ggtggcccag cgcgtatggc cgatgccgca cgttccaggc
     1681 aggtccttcg gcacggcctg cctgagctcg gctatcttgc cggtcttctt gatgacggac
     1741 aggggggctt ccccgtcctt gagcggctcg tccctgcgca cggctatgcc ggcagagtcg
     1801 taaccgcggt actcgaggcg cttgaggcct tccaccagta tcctggaggc ttgcctgggc
     1861 cccgtgtagc ctacgatccc gcacacggtg gtatcctttc cggccgcgtc cccgccgcga
     1921 atatcgccgg gaggcggcgc cggcgcgggg cgggtcgtgc ccgccgcgcg ctgggtagca
     1981 ggctatcgcg ccgcgaggcc ggcgtcaacc gggccttgcc gcgcgccgcc gccgcgagcg
     2041 ataatcgaag cgatggcaag actcaaacga cccaccggct tggggctcta cgtctccctg
     2101 accgtcgcgc tcataatgac ggcggagaca gccctcttcg tctcctactc gg
//
LOCUS       DAOOLN010000243         2149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_64250_length_2149_cov_3.930755, whole genome shotgun sequence.
ACCESSION   DAOOLN010000243 DAOOLN010000000
VERSION     DAOOLN010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64250_length_2149_cov_3.930755"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /gene="mutL"
                     /locus_tag="PLW80_03965"
     CDS             complement(<1..491)
                     /gene="mutL"
                     /locus_tag="PLW80_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPB65691.1"
                     /translation="MNASRIRVLAPEVAKRIAAGEVIDRPAAALRELLDNAIDSGAGS
                     VVVELEGGGIDSLRVVDDGSGMGKDDLALSVLPHATSKIRELDDLLSLSTLGFRGEAL
                     SSMAAVAGLEIVSATDDDEAWRLVSEPGRPATVTPSRGSRGTAVTLRDLFASFPARRQ
                     FMKR"
     gene            complement(585..>2149)
                     /locus_tag="PLW80_03970"
     CDS             complement(585..>2149)
                     /locus_tag="PLW80_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65692.1"
                     /translation="ASQATGPTDALASDFSPAEPPAADRSDDDATADKAASEAGGKVA
                     EWLRDVLGMEIGSITINGETWAKAVIQPTFAVGKLKTGLYLPVIYSSNLFDPSTWYRP
                     KGNDEWSFGTDVGWTAETWPEALLDAASDLALKIKFIEYGKPLVDPFFVKVGNLDSFT
                     IGHGLLMRDYANDTDFPSIRRVGLNLGLDQGSWGFEAMTNDLTDNQIVGGRLYIRPIP
                     GFKLALGLSGVADLYPASDYGDDAVLYGDPAFIGGALDLDLPIVTSGLLGIRLFADGA
                     AMAPYVRNAFSYGGFDAADGFRYDMVWTGDSIKNWGASAGLLGNVLFVDWRLEYRYYT
                     GAFTPAFFDAGYERRRSSLVSEWAGYLSGAEEIAQSISTMGVYGEAGASILKDKLSFA
                     LGYFWPWSPDASGLAEQLELTEDYLKATLTVKKGLIPVIDLAGSVSYERKNFVGTLAG
                     DADGVSLFDENTTFSGELSVPIPGAPNLDLAIIVSTTVARGDSGEVLFRDDDPTKPVI
                     VPAITLETRLHF"
BASE COUNT          347 a          676 c          766 g          360 t
ORIGIN      
        1 cgcttcatga actgtctgcg ggcggggaac gaggcgaaga ggtcgcgcag ggtcacggcg
       61 gtgcccctcg agccgcgcga gggggttacc gtcgccggcc tccctggttc ggataccagg
      121 cgccacgcct cgtcgtcgtc agtcgcgctg acgatctcca agccggcgac tgccgccatg
      181 gacgacaggg cctcgcccct gaagccgagg gtcgacagcg acaggaggtc gtcgagctcg
      241 cgtatcttcg aggtcgcgtg aggcaggacc gagagagcca ggtcgtcctt gcccatgcca
      301 gagccgtcgt cgacgacccg gaggctgtcg atgcctccgc cctccagctc gacgaccacg
      361 ctcccggcgc cggagtcgat ggcgttgtcc aggagttcgc ggagcgcggc ggccggtcgg
      421 tctatcacct cgccggccgc gatacgtttg gcaacctcgg gcgctagtac ccgtatcctt
      481 gaggcgttca tgctgtctgt atacaccctg gcggccgtac ctgtcacccg cgcgatagaa
      541 agggccgccc acgcgggcgg cccaagctac gcggatgccg gaggctagaa gtgcagcctc
      601 gtctcgagcg tgatggccgg gacgatgacc ggcttggtcg ggtcgtcgtc cctgaacagc
      661 acctcgcctg agtcgccgcg ggcgacggtg gtggatacga tgatggccag gtcgaggttc
      721 ggcgcgccgg gtatgggcac cgacagttcg ccggagaacg tggtgttctc gtcgaatagc
      781 gatacgccat ccgcgtcgcc ggccagggtg cccacgaagt tcttgcgctc gtaggatacg
      841 gagccggcca ggtcgatgac cggaatcagg cccttcttga ccgtaagcgt cgccttgagg
      901 tagtcctcgg tcagctcgag ctgctcggcc aggccggagg cgtccggcga ccagggccag
      961 aagtagccga gggcaaagct cagcttgtcc ttgaggatgc tggctccggc ctcgccgtag
     1021 acgcccatcg tcgagatgga ctgggctatt tcctcggcac cggacaggta gcccgcccac
     1081 tcggatacga gcgacgaacg cctgcgctca taaccggcgt cgaagaaggc cggcgtgaag
     1141 gctcccgtgt agtagcggta ctcgagcctc cagtctacga acagcacatt gcccaggagg
     1201 ccggccgacg cgccccagtt cttgatcgag tcgccggtcc agaccatgtc gtaacggaaa
     1261 ccgtcagcgg cgtcgaagcc accgtagctg aaggcgttcc tgacgtacgg ggccatcgcg
     1321 gcgccgtcgg cgaagagccg tatgccgagg aggccactcg tgacgatggg caggtcgagg
     1381 tccagggcgc cgcctatgaa ggccgggtcg ccgtagagga ccgcgtcgtc gccgtagtcg
     1441 gaagcggggt agaggtcagc gacgcctgac aggccgaggg ccagcttgaa gccggggatg
     1501 gggcgaatgt agagccggcc gccgacgatc tggttgtcgg tcaggtcgtt ggtcatcgcc
     1561 tcgaagcccc aggatccctg gtcgaggccg aggttcaggc cgacgcggcg tatcgacggg
     1621 aagtccgtgt cgttcgcata gtcgcgcatc aggaggccgt ggcctatcgt gaagctgtcc
     1681 aggttgccga ccttgacgaa gaaggggtcg acgagcggct tgccgtactc gatgaatttt
     1741 atcttgagcg ccaggtcgga ggcggcgtcg agcagggcct cgggccaagt ctcggcggtc
     1801 cagcccacgt cggtgccgaa ggaccactcg tcgttgccct tgggccggta ccaggtggac
     1861 gggtcgaaca ggttgctcga gtagatgacc ggcaggtaga ggccggtctt gagcttgccg
     1921 accgcgaagg tcggctggat gacggccttg gcccaggtct cgccgttgat cgtgatcgag
     1981 cctatctcca tgccgagcac gtcgcgcagc cactcggcga ccttgcctcc ggcttcggac
     2041 gcggccttgt cggccgtggc gtcgtcgtcg cttcgatcgg ccgcgggagg ctccgccggc
     2101 gagaagtccg acgcgagcgc gtcggtcggg ccggtcgcct gggaggcct
//
LOCUS       DAOOLN010000244         2149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_64263_length_2149_cov_3.051576, whole genome shotgun sequence.
ACCESSION   DAOOLN010000244 DAOOLN010000000
VERSION     DAOOLN010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64263_length_2149_cov_3.051576"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="PLW80_03975"
     CDS             <1..701
                     /locus_tag="PLW80_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65693.1"
                     /translation="RSGHYQLCVYGRTLALDDAAWARLARLYRSDASFKARVDESARR
                     VLRAKLRWLLPLGRDGVRPDDAPEAAMRSEESRTFFVEQAMRSATVLGRSELPLPETA
                     RVLVAGPFGDFTAEASARVPRATLYRFSYQPSADGLASELASFRARLPAADAVVVCVA
                     NPEGAAFARAARKAGKRLYVVSALNPAYAMEFKDDATVVAVYSYARESFKAAFAVLTG
                     DIEAIGRLPIEAGS"
     gene            893..1627
                     /locus_tag="PLW80_03980"
     CDS             893..1627
                     /locus_tag="PLW80_03980"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB65694.1"
                     /translation="MAVLLPAGGSAFPVLGGSYEGGGDDAAEALGGLIRSRAVRTYRP
                     AACVGASDHVAALEAALGWRPALEVEYDAMRLLPEARPAGGRRLDGGRRLAAHGFRSG
                     PAEIRRAGPDDLDALYPIAAAYEQAEVMTELHAFDPEACRAAQARSLASQLVYIAIVG
                     GRPVARAQTNARGLATDQIGGVYVEPERRGLGLGRLVVEALVAAILSDGRAASLFVKK
                     TNAIARSLYLSMGFVYARDYRVSYFA"
     gene            complement(1633..>2149)
                     /locus_tag="PLW80_03985"
     CDS             complement(1633..>2149)
                     /locus_tag="PLW80_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65695.1"
                     /translation="AETIELRTGDGTAEILVLDSRSFGLSVGLPATDDGQPLDRRYGS
                     GGLGLAAAPGAGVAMSLRVGGERVEVALYDGPRGVRGGARRGRSESARVDAIAIARHE
                     LRVRRGSADPILAAAASTAAAVVADYADRETVVVVRGDRLVVQWPEGGPVFVATAPEY
                     CLSGEFWLPEE"
BASE COUNT          294 a          788 c          764 g          303 t
ORIGIN      
        1 cgcggagcgg gcactaccag ctctgcgtct acggccgcac gctcgcgctc gacgacgccg
       61 cctgggcccg cctggccagg ctgtaccgaa gcgacgcttc gttcaaggcg cgagtcgacg
      121 agtcggccag gcgcgtgcta cgcgccaagc tccgctggct cctgccactc ggccgagacg
      181 gggtcaggcc cgacgacgcc ccagaagccg cgatgcgcag cgaagagtcg cgcacgttct
      241 tcgtcgagca ggcgatgcgc agcgccacgg tgctcggccg ctccgagcta cccctcccgg
      301 agacggcccg ggtgctggtc gccggcccct tcggagactt caccgccgaa gcgtccgccc
      361 gcgtgcctcg ggctacgctc tacagattct cgtaccagcc gagcgctgac ggactggcct
      421 cggagctggc gtcgttcagg gctcggctcc cggcggccga cgcggtcgtc gtctgcgtgg
      481 ccaaccccga gggcgcggcc ttcgcgcggg cggcccgcaa ggcgggcaag cggctctacg
      541 tcgtttcggc gctcaacccg gcttacgcga tggagttcaa ggacgacgcc acggtcgtgg
      601 cggtctattc ctacgcgcga gaatcgttta aggcggcctt cgcggtactg acgggagaca
      661 tagaggcgat aggtcgcctg cccatagagg ccggatcctg agccccgagg gctggcggag
      721 ggccgcggac ggcgacgcgc ccgccctccg gcgcttcctg gagccccgcg agggctacgc
      781 cacaggattc tcgtataagg cgttctcggc ccatggcgag ctgactcgta tccgtcccga
      841 tcggggcggc ctgttcgtac gtcccgacga ggaccgcgcc gatcgcttgt cgatggcggt
      901 gctgttgccg gccggaggct cggcgttccc ggtgctcggc gggagctacg aaggcggcgg
      961 cgacgacgcc gcggaggcgc tcggcgggct aatccgctcg cgggcggtac ggacctaccg
     1021 cccggccgcc tgcgtcgggg cgtccgacca cgtggccgcc ctcgaggcgg ccctgggctg
     1081 gcgcccggcg ctcgaggtcg agtacgacgc gatgcgcctc ttgcccgaag cccggccggc
     1141 cggcggtcgg cggctggacg gcggtcggcg gctggcggct cacggcttca gatccggtcc
     1201 ggccgagatc agacgagccg ggcccgacga cctggacgcg ctctacccca tcgcagccgc
     1261 ctacgagcag gccgaggtga tgaccgagct gcacgccttc gaccccgagg cctgccgcgc
     1321 cgcccaggcg cggagccttg cgagccagct agtctatatc gcgatcgtag ggggccgccc
     1381 ggtagccagg gcccagacga acgcccgcgg cctagcgacc gaccagattg gcggcgtcta
     1441 cgtcgaaccg gagcgccggg gactcggcct cgggaggctc gtcgtcgagg cgcttgtcgc
     1501 ggctatccta tcggatggga gggcggcctc gctgttcgtc aagaagacga acgccatcgc
     1561 ccgctcgctc tacctgtcga tgggcttcgt ctatgcccgg gactaccgag tctcgtactt
     1621 cgcctgagcc ggtcattcct cgggcagcca gaattccccg gacaggcagt actccggagc
     1681 cgtagcgacg aagacgggcc cgccctcggg ccactggacg acgaggcggt cgcctcgcac
     1741 gacgacgacg gtttcccggt cggcgtagtc ggcgaccacg gcggccgccg tcgaggcggc
     1801 ggccgccagt atgggatctg cggaacctcg gcgcacccgt agctcgtgcc tggcgatggc
     1861 gatcgcgtcc accctcgccg actctgaccg gccgcgccgg gcaccgcccc gaacgcccct
     1921 cgggccgtca tacagcgcca cctccacccg ctccccgccg actctcagcg acatggccac
     1981 gccggcgccg ggggcggccg cgaggccgag gccaccggaa ccatagcgcc ggtcgagcgg
     2041 ctggccgtcg tcggtggcgg gcaggccgac cgacaggccg aagctcctcg agtcgagcac
     2101 gagtatctcc gcggtcccgt cgccggtccg cagctctatg gtctcggcg
//
LOCUS       DAOOLN010000245         2141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_64603_length_2141_cov_2.646213, whole genome shotgun sequence.
ACCESSION   DAOOLN010000245 DAOOLN010000000
VERSION     DAOOLN010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64603_length_2141_cov_2.646213"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(378..1658)
                     /gene="murA"
                     /locus_tag="PLW80_03990"
     CDS             complement(378..1658)
                     /gene="murA"
                     /locus_tag="PLW80_03990"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPB65696.1"
                     /translation="MNQYRIEGGHPLKGRIRVSGNKNAALPCIAAALLAEEPVTLRNV
                     PEIEDVFVMLDICRSLGASVDRLGPNDWTIDGRAVGLPEVPASLAARVRASILFAGPL
                     LARFGRVTLPPPGGDVIGRRRLDTHFLALEELGARIEVDGSFQFTANKLKGADIFLDE
                     ASVTATENAVMAASIAEGRTVIRNAASEPHVQDVCRLLLAMGASISGIGSNILTIDGA
                     AALHGADFTIGPDFMEVGSFIGLAAVTRGDIVIEGVAPDDLRMMKLAFGKLGVTWSLD
                     GDALSVHSGQGMRVVPDLGGQIPKIDDAPWPGFPPDLTSIMTVVATQAEGTVLIHEKM
                     FESRMFWVDKLIGMGARIVLCDPHRAVVSGPARLRGDSLTSPDVRAGMALVIAALCAE
                     GTSVIKNVYQIERGYERIAERLQALGAKVERLKE"
     gene            1821..>2141
                     /locus_tag="PLW80_03995"
     CDS             1821..>2141
                     /locus_tag="PLW80_03995"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPB65697.1"
                     /translation="MSITRRNPVPSDIEIAQEARIKPIVQVAEDYGICDECIDHFGKY
                     KAKVRLEFLRQNADAPKRAKYIDVTAISPTPLGEGKTTTTVGLTQGLGRIGKKAIAAI
                     RQPSM"
BASE COUNT          371 a          684 c          720 g          366 t
ORIGIN      
        1 gcaccgtcat ggtggccgac tgtttgccgg tgttgatcac cgacacggcg ggccgcgcgg
       61 tggccgccgc gcacgccggc tggcgtggcc tggcgggcgg cgtgctggag cgtgcgttgg
      121 agtcgtttcg agcgtgtgcg ctggctgcat cagcgtctga agctataaaa aatgaagcaa
      181 tcgatctgat ggcctggctc ggtccgtgca ttggtccgcg gtccttcgag gtcggacccg
      241 acgtgcgcgc cgccttcgtc gacgccgatg ccggcgccgc cgcgtgcttt gccccgcaac
      301 ccggcggcaa gtacctggcc gacttgcccg cgctggcgcg gcgccgattg gcggccgccg
      361 gcggcaacga aacgcgacta ctccttgagg cgctccacct tcgccccgag cgcctggagc
      421 ctctcggcga tgcgctcgta gccgcgctcg atctggtaaa cgttcttgat gacgctcgtg
      481 ccctcggcgc agagcgccgc gatgacgagg gccataccgg cgcgcacgtc gggcgaggtc
      541 aacgagtcgc cgcgcaggcg cgccggcccg gagacgacgg cgcggtgcgg atcgcagagc
      601 acgatcctgg ctcccatgcc tatcagcttg tcgacccaga acatgcgcga ctcgaacatt
      661 ttctcatgga tcagtacggt accttcggcc tgagtcgcca cgaccgtcat gatcgaggtg
      721 agatccggcg ggaagcccgg ccacggcgcg tcgtcgatct tgggtatctg tccgccgagg
      781 tcgggtacca cgcgcatgcc ctggcccgag tgcaccgaca gcgcgtcgcc gtccaggctc
      841 caggttacgc ccagctttcc gaaggccagc ttcatcatgc gcaggtcgtc gggagcgacg
      901 ccctcgatga cgatgtcgcc tctggtcacg gcggctagcc cgatgaacga gccgacctcc
      961 ataaaatccg gcccgatcgt gaagtcggcg ccgtgcaggg cggcggctcc gtctatcgtc
     1021 aggatgttcg agccgatgcc ggagatagac gcgcccatcg ccaggaggag cctgcagaca
     1081 tcctgtacgt gcggctcgct cgcggcgttg cgtatcacgg tgcggccctc ggctatggag
     1141 gcggccatga cggcgttctc ggtggccgtg acggaggctt cgtcgaggaa gatgtcagcg
     1201 cctttgagct tgttggcggt gaactggaac gagccgtcca cctcgatgcg cgccccgagc
     1261 tcctcgagcg ccaggaaatg cgtgtcgagg cgcctgcgac ctatgacgtc gcctcccgga
     1321 ggcggcaggg tcaccctgcc gaacctggcc aggagggggc cggcgaacag gatggaggcc
     1381 cggactctcg cggcgaggct cgcaggcacc tcgggcaggc cgacggcgcg accgtctatc
     1441 gtccagtcgt taggaccgag cctatcgaca gaagcgccaa gggacctgca gatgtcgagc
     1501 atcacgaaga cgtcctcgat ttcgggtaca ttcctgagcg ttaccggctc ctcggccagt
     1561 agagcggccg ctatgcatgg cagggcggcg ttcttgttgc cgctgacacg tatcctgcct
     1621 ttgagcgggt gaccgccctc gatacggtac tggttcatgt tggcatccta accggggcgg
     1681 ccgtggccgt caacgtcggg ggcgccgatg ccgcaatgct gccgcgttcg tactaatcgg
     1741 tttgacgcgg atgccggcct tcgctacctt taagcgacgg atcaaacgaa tccgggacgc
     1801 gcgaagccct aggaggtcat atgtccataa ccagaaggaa cccggtaccc agcgatatcg
     1861 agatcgccca ggaagcgagg atcaagccaa tcgtgcaggt agccgaggat tacggcatct
     1921 gcgacgagtg catcgatcat ttcggcaagt acaaagcgaa ggttcgactg gagttcctga
     1981 ggcagaacgc cgacgcgccg aagcgcgcca agtacataga cgttaccgcg atcagcccta
     2041 cgcctctcgg cgagggtaag accacgacga cggtaggtct gactcagggc cttggccgga
     2101 taggaaagaa ggccatcgcc gcgatcaggc agccgtcgat g
//
LOCUS       DAOOLN010000246         2134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_64906_length_2134_cov_2.924002, whole genome shotgun sequence.
ACCESSION   DAOOLN010000246 DAOOLN010000000
VERSION     DAOOLN010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64906_length_2134_cov_2.924002"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..598
                     /locus_tag="PLW80_04000"
     CDS             5..598
                     /locus_tag="PLW80_04000"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HPB65698.1"
                     /translation="MYIATGNAHKLDEIRPALPGVDVRLPSDAGVDFDYEETAADFVG
                     NSIGKAMALWNAVGSPVLADDSGLCVDALGGRPGVLSARYGSPDGVTPLGAAERNARL
                     LGEMRGRSDRSCRFVCCMTLVLSRDRFFVIQEACEGVLLEEPRGSGGFGYDPLVLLPE
                     LGRSVAELSVEEKNRVSHRGRALVRVSSLLGSLVAAP"
     gene            complement(693..896)
                     /gene="rpmE"
                     /locus_tag="PLW80_04005"
     CDS             complement(693..896)
                     /gene="rpmE"
                     /locus_tag="PLW80_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPB65699.1"
                     /translation="MKSGIHPKYEFTTITCACGNVIETRSTVKNIQVEICSACHPFFT
                     GKQKLVDTAGRIDRFRRKYGIKE"
     gene            complement(972..>2134)
                     /gene="rho"
                     /locus_tag="PLW80_04010"
     CDS             complement(972..>2134)
                     /gene="rho"
                     /locus_tag="PLW80_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPB65700.1"
                     /translation="IFQILKAHTERGGIIYAYGSLEILPDGYGFLRSPQNSYLPASDD
                     IYISPSQIRLFNLKTGDTVFGQIRSPKEGERFFAMLRVEQVNFDEPAVAQNRIPFENL
                     TPLYPNQRLNLETTTAETSTRIINLFCPIGKGQRALIVSPPKTGKTILLQKIANAITA
                     NHPEVYLIVLLIDERPEEVTDMERTVKAEVISSTFDEQATRHVQVSEMVLDKAKRLVE
                     HGKDVVILLDSITRLARAYNQTVPTSGKILSGGVDSNALHKPKRFFGAARNIEGGGSL
                     TIIATALVDTGSRMDEVIFEEFKGTGNMEINLDRRMSDRRLFPAINVKKSSTRKEELL
                     LASDELQKIWILRKALSPMDDLEATELLIDKMSKTKNNEAFLRSMNTPLGDS"
BASE COUNT          363 a          623 c          687 g          461 t
ORIGIN      
        1 gaccatctat atagcgaccg ggaacgccca taagctcgac gagatacggc cggcactgcc
       61 gggcgtcgac gtacgactgc cttcggacgc gggcgtcgac ttcgactacg aggagaccgc
      121 cgccgatttc gtcggcaact ccatcggcaa ggccatggcc ctatggaacg cggtcggctc
      181 cccggtcctc gccgacgatt cgggcctctg cgtcgacgcg ctcggcggca ggccgggagt
      241 gctgtcggcg cgctatggct cccctgacgg cgtaacgccg ctcggcgccg ccgagcgcaa
      301 cgcgcggtta ctcggagaga tgcgcggacg gagcgaccgc tcctgccgtt tcgtatgctg
      361 catgacgctg gtcctgtcta gggataggtt cttcgtgatt caggaggcct gcgagggcgt
      421 cctgctcgaa gaaccacggg gatccggcgg tttcggctat gacccgttgg tactgctacc
      481 cgaactcggc cgtagcgtcg cggagctgtc ggtcgaggaa aagaaccgcg tgtcgcatcg
      541 cggaagggcc ctcgtccggg tctcgtcgct actgggtagc ctggtcgcgg ctccataatc
      601 cggcgaccga ctcgcggccc aaaaaaaggc cggcagctct accgagcgcc ggccttcttc
      661 gtttcgctcc cgcgggcgca agccccaggg cgttactcct tgataccgta cttcctgcgg
      721 aagcggtcga tgcgtcctgc agtgtctacg agcttctgct ttccggtaaa gaaggggtgg
      781 caggcggagc aaatttccac ctggatattc tttaccgtcg aacgggtctc aatgacattg
      841 ccgcacgcgc acgtgatcgt ggtgaactcg tacttgggat gtatcccgct cttcatcgtg
      901 atctcctcgc gtgccgctca tacatgccag aataccggct tgatgacggc taaaagtcag
      961 gtcccgccga ctcaggaatc gcctaagggc gtattcatgg atcgtaggaa tgcctcatta
     1021 ttcttagttt tggacatctt gtcaatcagc agctcggtag cctcgaggtc gtccatcggg
     1081 ctcagggcct tgcgtagtat ccatatcttc tggagctcgt ccgaggcgag gagtagctcc
     1141 tccttgcggg tgctcgactt cttgacgttg atcgctggaa agagcctacg gtccgacatc
     1201 cgcctgtcca ggttgatctc catattgccg gtgcccttga attcctcgaa gatcacctcg
     1261 tccatcctgg aacccgtgtc gacgagggcc gtcgcgatga tggtcaacga gccgccgccc
     1321 tctatgttgc gcgcggcgcc gaagaatcgc ttgggcttgt ggagggcgtt cgagtcgacg
     1381 ccgcctgaca gtatcttgcc ggaggtcggc acggtctggt tataggcccg cgcaagcctc
     1441 gtgatcgagt cgagcaggat cacgacgtcc ttgccgtgtt cgacgaggcg cttggccttg
     1501 tccaagacca tctcggagac ctgtacgtgg cgggtggcct gctcgtcgaa ggtcgacgat
     1561 atgacctccg ccttgacggt gcgttccatg tcggtcacct cctccggccg ctcgtcgatg
     1621 agcagcacga tcaggtagac ttcggggtgg ttggccgtga tggcattggc tatcttctgt
     1681 agaagtatcg tcttaccggt cttcggcggg gagacgatca gcgctcgctg tcccttgccg
     1741 atcggacaga acaggttgat gatgcgcgtg gaggtctcgg ccgtggtcgt ctcgaggttg
     1801 agacgctggt ttggatagag cggagtcagg ttctcaaacg gtatgcgatt ctgggcgacg
     1861 gccggctcgt cgaagttgac ctgctcgacg cggagcatgg cgaagaatcg ctcgccttcc
     1921 ttggggctac ggatctgtcc gaagacggta tcgccggtct tcaggttgaa gagcctgatc
     1981 tgggacgggc tgatgtagat atcgtccgag gcgggcaggt agctgttctg gggggagcgc
     2041 aggaagccgt aaccgtccgg cagtatctcg agcgagccat aggcgtagat gatgccgccg
     2101 cgctcggtat gggccttcag tatctggaag atga
//
LOCUS       DAOOLN010000247         2133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_64964_length_2133_cov_2.632339, whole genome shotgun sequence.
ACCESSION   DAOOLN010000247 DAOOLN010000000
VERSION     DAOOLN010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64964_length_2133_cov_2.632339"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PLW80_04015"
     CDS             <1..826
                     /locus_tag="PLW80_04015"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB65701.1"
                     /translation="LAARGDTAGAIKSYQAALSFEPDNVAALRGLGVVYSSTGKGAEA
                     EKVFARAVELAPDAVTYYNLAKVKYDLGKAAEALPLARKAVELGPSVALYQYQLGLTA
                     ERAGDVDAAISAYAKAAELDKKGVDARVNLGRLYLESGFVDKALAMLEEAYRVDPKSL
                     EANNNLANAYGTKGLFDKSVFHYEAALALAPRDATIRLNLARAYVQSGALPKARDAYI
                     ELLKLAPATWEAMYELGKAYLSLGDQASAKKTLSELLAKKPDFRARVEVETILSGL"
     gene            complement(852..>2133)
                     /locus_tag="PLW80_04020"
     CDS             complement(852..>2133)
                     /locus_tag="PLW80_04020"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65702.1"
                     /translation="AEAGACRAVFVDKAMAFPDVAFRLSSGGRPLLSLRPSSLAERVI
                     AAQAPDQAPELFRELVASGDGFSARIVAGLPSVYRTDRRHIQVFVNGRRVQEYGLSQA
                     IEYAYRGALPGGAWPFAYAFVEVDPRLADFNIHPAKKEVRLRNLDEIRSGIIRAIRDY
                     LGAQSRLASPFMPPPSLSGRPPAVEASSAEGLFSSAAFVPEARERPYPADRSSWSSIA
                     DAAAKARETPRRAAGDVTPVGGRDDGLENGFRYIGRALGVFLAFERGDELVLLDQHAA
                     HERYLYDEMMAGRAVSQELLVPVVYEPESEAEDRYIAAATETLAQAGYRLSKEGGAWL
                     LEAAPAMLPEAMTGSVFALLRSRPDPAELLRETVAQSACKAAVKDGDELDETGAIALI
                     EAALSMPEPRCPHGRPIWYRLSRDDLFRAVRRLV"
BASE COUNT          363 a          717 c          720 g          333 t
ORIGIN      
        1 cctggcagcc cgaggtgaca cggctggcgc catcaaatcg taccaggcgg cgctatcgtt
       61 cgagccggac aacgtggccg cccttcgtgg ccttggagtg gtctactcgt cgaccggcaa
      121 gggcgccgag gccgagaagg ttttcgcccg ggcggtcgag ctggctcccg acgccgtcac
      181 ctactacaac ctcgccaagg tcaagtacga cctcggcaag gccgccgagg ccctgccgct
      241 cgctcgcaag gctgtggaac tcggtccgtc ggtcgcgttg taccagtacc agctcggact
      301 caccgccgag cgcgccggcg acgtcgacgc tgcgatctcg gcctacgcca aggccgccga
      361 gctcgacaag aagggcgtgg acgcccgcgt caacctcggt cgcctatacc tggagtcggg
      421 cttcgtcgac aaggccctgg ccatgctcga ggaagcctac cgtgtcgacc cgaagtcgct
      481 cgaggcgaac aataaccttg ccaacgccta cggcaccaag ggcctgttcg acaagagcgt
      541 gttccactac gaggcggccc tggccctggc cccgcgcgac gcgacgatac gtctgaatct
      601 ggcccgcgcc tacgtgcagt cgggcgcgct acccaaggcg cgggacgcct atatcgagct
      661 attgaagctg gcccccgcta cctgggaggc catgtacgaa ctgggcaagg cctacctgtc
      721 gctcggcgac caagcctcgg ccaagaagac cctgtccgag ctgctcgcca agaagcccga
      781 cttcagggcc agggtcgagg tggagacgat tctgtccggg ctctgagacg ccgcccctcg
      841 gcgagcctat tttagaccag gcgtcggact gccctgaaca ggtcgtcccg cgacaatcta
      901 taccagatgg gcctgccgtg ggggcaacgc ggctcgggca tggagagcgc cgcttcgatg
      961 agggcgatcg cgccagtctc gtcgagctcg tcgccgtcct tgacggctgc cttgcacgct
     1021 gactgggcca ccgtctcgcg cagaagctcg gccgggtccg gccgtgagcg tagcaacgcg
     1081 aacacggacc cggtcatcgc ctcgggcaac atcgccgggg ctgcctcgag tagccaggct
     1141 ccgccttcct tcgataaccg gtagccagcc tgggcgagcg tctcggtcgc cgcggctata
     1201 tagcggtctt cggcctccga ctccggttcg tagacgaccg ggacgagcag ttcctgggat
     1261 acggcgcggc cggccatcat ctcgtcatac aagtagcgct cgtgggccgc gtgctggtcg
     1321 agtagcacga gctcgtcgcc ccgttcgaag gcgaggaaga cgccgagcgc cctgcctatg
     1381 tacctgaagc cgttctcgag cccatcgtcg cggcctccga cgggcgtaac gtcgccagcc
     1441 gcgcggcgcg gcgtctccct ggccttggca gctgcgtccg cgatcgagga ccagcttgaa
     1501 cggtcggcag ggtagggccg ttcgcgggct tccgggacga aggcggcgga actgaacaaa
     1561 ccttcggcgc tactcgcctc gacggcaggc ggccggcctg atagcgaagg cggaggcatg
     1621 aatggcgagg ctagcctcga ctgggcaccc aggtaatcgc gaatggcgcg tatgatgccg
     1681 gagcggatct cgtcgaggtt cctgagcctg acttcctttt tggccggatg gatgttgaaa
     1741 tcggctagcc tcgggtcgac ctcgacgaag gcgtaggcga agggccaggc gccgccgggt
     1801 agagcgcctc gatacgcgta ctcgatggcc tgggagaggc cgtactcctg gacgcgacgg
     1861 ccgttgacga aaacctggat atggcgccga tcggtacggt agaccgacgg caggcccgcc
     1921 acgatcctcg ccgagaagcc atctcccgag gcgacgagct ccctgaacag ctccggcgcc
     1981 tggtcggggg cctgggcagc gatgacgcgc tcggccaggc tcgacggccg gagcgaaagg
     2041 agcggccgtc cgccggagga tagcctgaag gccacgtccg ggaaggccat cgccttgtcg
     2101 acgaagacgg ccctgcaggc tcccgcctcg gcg
//
LOCUS       DAOOLN010000248         2130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_65092_length_2130_cov_2.505542, whole genome shotgun sequence.
ACCESSION   DAOOLN010000248 DAOOLN010000000
VERSION     DAOOLN010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65092_length_2130_cov_2.505542"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PLW80_04025"
     CDS             <1..554
                     /locus_tag="PLW80_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB65703.1"
                     /translation="VVTGFLWKLMLDPNFGPINLLLDRVGLGALALPWLGLEETTLPI
                     ISLVSSWQWVGIPTMIFTAALQSIPEDYFEAAQIDGASGWQVTRLIKLPIIVPMMGVV
                     GVLTFTGNFNAFDVVYSMAGANGAPNYAADILGTYFYRTGIAGKHPVGIPDMGLGAAI
                     ATVTFVTLLAGVMVIRRLTMGRK"
     gene            574..1422
                     /locus_tag="PLW80_04030"
     CDS             574..1422
                     /locus_tag="PLW80_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB65704.1"
                     /translation="MNDGGSARRRLRASRMGSYLILCAWAVVVLVPLYLMIVNSFKST
                     SDIFMSPFSFPKNPVFDGFRMVFEEGHFLLYLFNSVLITVLAIVGVMLLAALASYALV
                     YWDVRLARAVSLFFLLGLMIPMRIASINLIIMLKGMGLLDKLLGLLPIYLAMGMPMGV
                     MILTEYFRTIPRELVQAAYIDGAGSFRIFRSVMLPLARPAMATVAIFNLVTIWNDLWF
                     PLILIRSEGQRTLMLGVTRLFGQFQTDWTSVLATLTLATAPILALYLLLSRQFIEGLT
                     AGAIKE"
     gene            complement(1435..>2130)
                     /locus_tag="PLW80_04035"
     CDS             complement(1435..>2130)
                     /locus_tag="PLW80_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB65705.1"
                     /translation="GQGLGAARRYPGGLMTRILIVDDQRLFAESLRTVLEGTQDDFRV
                     CALAGDGAQAVAAVAEHQPDIVLMDVSMPAMDGVEAARIIHNRYPELPILMLTTFEDD
                     GYVREAIAYGARGYLLKNIPSRELVASIRAVLSGAMLLDPRVVGALVGKGRDGPASGA
                     RAASPEWFDSLSRRERQILGLMVDGLQNDEIAQRLCVAPQTVRNYVSRLYDKTATGDR
                     LRLIRLARASGLF"
BASE COUNT          329 a          704 c          698 g          399 t
ORIGIN      
        1 tcgtcgtcac cggcttcctc tggaagctga tgctcgatcc caacttcggc ccgatcaacc
       61 tcctgctcga tagggtgggc ctcggcgccc tggccctgcc gtggctcggg ctcgaggaga
      121 cgaccctgcc gatcatctcg ctcgtgtcga gctggcaatg ggtcggcatc ccgacgatga
      181 tctttaccgc cgcgctccag agcatcccgg aggactattt cgaggcggcc cagatcgacg
      241 gcgcctccgg ctggcaggtg acgaggctca tcaagctgcc gatcatcgta ccgatgatgg
      301 gcgtggtcgg cgtgcttacc tttacgggga acttcaacgc gttcgacgtg gtctactcga
      361 tggccggtgc gaacggggcg ccgaactacg cggccgacat actcggcacg tacttctacc
      421 ggaccggcat cgccgggaag catcccgtcg gtatccctga catgggcctc ggggccgcga
      481 tcgcgacggt caccttcgtc acgctgctcg ccggcgtgat ggtcatacgg cgcctgacca
      541 tgggcaggaa gtaagggggc gacgatgccg agagtgaacg atggcgggag cgcccggagg
      601 cggctccgcg cctcgaggat gggctcctac ctgatactct gcgcgtgggc ggtcgtcgtg
      661 ctcgtgccgc tctacctgat gatagtcaac tcgttcaagt cgacttcgga catcttcatg
      721 tctcccttca gcttcccgaa gaacccggtc ttcgacggct tccgcatggt cttcgaggag
      781 gggcatttcc tcctgtacct gttcaactcg gtgctcatca cggtgctggc catcgtcggc
      841 gtgatgctcc tcgcggcgct cgcgagctac gcgctggtat actgggacgt gcggctcgcc
      901 agggccgtat cgctgttctt cctgctcggg ctgatgatcc cgatgcgcat agcgagcatc
      961 aacctgatca tcatgctcaa ggggatgggc ctgctcgaca agctgctcgg gctcctgccg
     1021 atctacctgg cgatgggcat gcccatgggc gtgatgatac tgaccgagta cttccggacc
     1081 atacccaggg agctcgtcca ggccgcctac atcgacggcg ccggctcgtt ccgcatcttc
     1141 aggagcgtga tgctgccgct cgctcgcccg gcgatggcga cggtggcgat cttcaacctg
     1201 gtgacgatct ggaacgacct gtggttcccc ctcatcctga tacggagcga gggccagcgc
     1261 accctgatgc tcggcgtgac caggctgttc gggcagttcc agaccgactg gacgagcgtg
     1321 ctcgcgacgc tgactctggc gacggcgccg atcttggcgc tctacctgct cctgtccagg
     1381 cagttcatcg agggcctgac agccggcgcg atcaaggaat aggcgggacg ctagctagaa
     1441 caggccgctg gcgcgggcca gccgtatgag cctgaggcgg tccccggtgg ccgtcttgtc
     1501 gtagaggcgg ctgacgtagt tccggacggt ctgcggcgcg acgcagagcc gctgggctat
     1561 ctcgtcgttc tggaggccgt ccaccatgag gccgagtatc tggcgctcgc ggcgcgacag
     1621 cgagtcgaac cactccgggc tagcggcgcg agcccccgac gccggtccat cgcgcccttt
     1681 cccgacgagg gcgccgacga cgcgcgggtc gagcagcatg gcgccgctca gcacggcccg
     1741 gatgctcgcc acgagctcac ggctcgggat gttcttaagc aggtagccgc gggcgccgta
     1801 ggctatcgcc tcgcgcacgt agccgtcgtc ctcgaaggtg gtcagcatca ggatgggaag
     1861 ctcggggtag cggttgtgga tgatcctggc cgcctcgacg ccatccatcg ccggcatgct
     1921 gacgtccata agcactatgt ccggctggtg ctcggcgacg gcggccacgg cttgagcgcc
     1981 gtcgcccgcc aacgcgcaga ccctgaaatc atcctgcgtg ccctcgagca cggtcctgag
     2041 gctctcggcg aacagccgct ggtcgtccac gatcaggatc ctggtcatga gccctcccgg
     2101 gtaccgtcga gcggcgccga ggccctgacc
//
LOCUS       DAOOLN010000249         2129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_65139_length_2129_cov_2.544841, whole genome shotgun sequence.
ACCESSION   DAOOLN010000249 DAOOLN010000000
VERSION     DAOOLN010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65139_length_2129_cov_2.544841"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PLW80_04040"
     CDS             complement(<1..210)
                     /locus_tag="PLW80_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65706.1"
                     /translation="MYFNELVAAYDRERHERDTFHDLMRHRVREILLVASLYDAFVLE
                     SDGALSEQIYGEFFKLNLTTAPRVSC"
     gene            complement(316..885)
                     /locus_tag="PLW80_04045"
     CDS             complement(316..885)
                     /locus_tag="PLW80_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB65707.1"
                     /translation="MVSARDRILEASLELFSARGYSAVTTKAIAEAAAVNEVTVFRLF
                     GSKERLFREVFAAYYIKPDDAIRAISLRDELEGDLVAFARTFVAFFSGNHKIVSMSIK
                     DIKEEFADIDDALRGQVDIMRAAVSPYLAGAVGRGAAFGDPAELASLFVECLFGFAFH
                     RVRLGGIADLGSAVVAFARVFARGIATAS"
     gene            987..>2129
                     /locus_tag="PLW80_04050"
     CDS             987..>2129
                     /locus_tag="PLW80_04050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPB65708.1"
                     /translation="MNEPHKTDFLGRDAIFPLLLKMGIPAAVGMLVNALYNVVDTIFV
                     GHGVGPLAIAALSIVFPLQMIVSAVAQALGIGAASIVSRRLGEKRPDAAAAAMGTAYA
                     AVMLVTAVLVALVFLFTRPILAFFGASETIMPYALSYTRIVAAGFFFFSMSMCASNLI
                     RAEGNAKASMLGMLLGAGLNTILDPIFIFGFGMGVEGAAVATVISQVVSCAYLFSLYF
                     RKKSHVPIGVADLRIEPSVLRDEALLGVPAFVQSAGMSLLALLINTTLGRYGGDLAIT
                     TYGMNHKILSIVIMPILGIIQGFQPIAGYNYGAKRFDRVRQSLKTTVLTALAVSLAGY
                     AFMMLAPRAAIGLFTADAELIASSARVLRVMVLFIPLASLQITGSTY"
BASE COUNT          377 a          704 c          663 g          385 t
ORIGIN      
        1 gcaggacacc ctcggggcgg tggtcaggtt gagcttgaag aactcgccgt agatctgctc
       61 cgacagggcg ccgtcgcttt cgagcacgaa ggcgtcgtag aggctggcga cgagcaggat
      121 ctcgcgcacg cggtggcgca tcaggtcgtg gaaggtgtcg cgctcgtggc gctcccggtc
      181 gtacgcggcg accagctcgt tgaagtacat gacgctatcg tagcgcggct ccgcgcccct
      241 cgcaagagac gcccgcgagc gcctcgttga gcgcctcgtt gagcgcctcg ttgagcgccg
      301 ctggcaaacg cgcctctacg aggccgtagc tatgcccctg gcgaagacgc gggcgaaggc
      361 gacgacggcc gagccgaggt cagcgatgcc gccgagcctg acgcgatgga acgcgaaacc
      421 gaagaggcac tcgacgaaca gcgacgccag ctcggccggg tcgccgaacg ccgcgccgcg
      481 gccgacggcg ccggcgaggt acggggagac ggcggcgcgc atgatgtcga cctggccgcg
      541 cagcgcgtcg tcgatatcgg cgaactcttc cttgatatcc ttgatgctca tgctgacgat
      601 cttatggttg ccggagaaga acgcgacgaa ggtccgggcg aacgcgacga ggtcgccctc
      661 cagctcgtcg cggaggctta tcgcccgtat cgcgtcgtcg ggcttgatat agtacgccgc
      721 gaacacctcg cggaacagcc gttccttcga accgaacagc ctgaacacgg tgacctcgtt
      781 gaccgcggcg gcctcggcga tcgccttggt cgttacggcc gaatagccac gggcggagaa
      841 taattccaaa gaggcttcga gtatcctgtc cctcgcgctc accatgaccg tgcgactata
      901 tgatacagac cgcgataatg caagtaagcg cttgcatcat accgcaattt tcgctatggt
      961 accgcgaacc gataaggaac caagcgatga acgaacctca caagaccgat ttcctcgggc
     1021 gggacgctat cttcccgctc ctcctcaaga tgggcatacc ggccgcggta ggcatgctgg
     1081 tcaacgccct ctacaacgtc gtcgatacca tcttcgtagg ccacggcgta gggccgctcg
     1141 cgatagcggc tctatcgatc gtcttcccgc tgcagatgat agtctcggcg gtagcccagg
     1201 ccctcggcat cggcgccgcc tcgatcgtct ccaggcgcct cggagagaaa cggccagacg
     1261 ccgccgccgc cgcgatgggt accgcctatg cggcggtcat gctcgtcacg gcagtcctcg
     1321 tagcgttggt gttcctgttc acgcgaccca tcctggcctt cttcggggcc agcgagacca
     1381 tcatgcccta cgcgctcagc tacacccgca tcgtcgcggc cggcttcttc ttcttctcga
     1441 tgtcgatgtg cgcgagcaac ctgatacggg cggagggtaa cgccaaggcg tcgatgctcg
     1501 gcatgctgct cggcgccggc ctcaacacga tactcgaccc gatcttcata ttcgggttcg
     1561 gcatgggcgt ggagggagcg gccgtggcga cggtgatcag ccaagtcgtc tcgtgcgcct
     1621 acttgttctc cctgtacttc aggaagaaga gccatgtacc gatcggcgtg gccgacctgc
     1681 ggatcgagcc gtcggtattg cgcgatgaag ccctgctcgg cgtgcccgct ttcgtccaga
     1741 gcgccggcat gagcctcctg gccctgctga tcaacactac cctcggcagg tacggcggcg
     1801 acctcgcgat cacgacctac ggcatgaacc ataagatcct gtctatcgtc atcatgccga
     1861 tactcggcat catccagggc ttccagccca tcgccggata taactacggc gccaagcgtt
     1921 tcgaccgggt caggcagagc ctcaagacca ccgtactgac ggcgctggcg gtgtcgctgg
     1981 ccggctatgc gttcatgatg ctcgccccgc gcgcggccat cggcctgttc accgccgacg
     2041 ccgagctgat cgcctcgagc gcgcgggtgc tacgggtcat ggtgctgttc atcccgctcg
     2101 cgtcgctcca gataaccggc tcgacctac
//
LOCUS       DAOOLN010000250         2128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_65200_length_2128_cov_2.086831, whole genome shotgun sequence.
ACCESSION   DAOOLN010000250 DAOOLN010000000
VERSION     DAOOLN010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65200_length_2128_cov_2.086831"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PLW80_04055"
     CDS             <1..592
                     /locus_tag="PLW80_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HPB65709.1"
                     /translation="IAYHEAGHAIVAAFTPEADPVQKVSIVPRGFGALGFTLQVPIED
                     RYIVTEDKLLGEIDVLMGGRAAEELTFGKVSTGAANDITRATDIARRMITDYGMSERY
                     RNVALTKRGAGMMGPSAEPVMAREYGEETQRYIDETVAATVAARYEIARRTLDERRGL
                     LDTLAGELLEKESIDEAAFKAIVAPPRASDERLVSS"
     gene            690..1550
                     /locus_tag="PLW80_04060"
     CDS             690..1550
                     /locus_tag="PLW80_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPB65710.1"
                     /translation="MKSIHDSASLGLAALVAAAALALVSSCDRPRGDQAADEAPRVVY
                     SAHTQNYVPYGYIDEKGGSDGFEIAVLRAVDELLPDYEFRFVPTSDEDLFIGLETGRY
                     QLATKGAWATAERREKFVFPKNPIAASVIGIAFRSEDSGQIKDLDSFAAYSGKLVPIS
                     PQSAQYTVVSDYNAAHPEHPIKLVPSDVFIINDAYLWVLEGRYDAFFDIKLSFQKNVE
                     AEDGPFHHLASRLAYAPYKGIPTWPLFARGEQALADAYDEAIERLKAEGTLAELSRRY
                     FGEDVFRFAE"
     gene            1558..>2128
                     /locus_tag="PLW80_04065"
     CDS             1558..>2128
                     /locus_tag="PLW80_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPB65711.1"
                     /translation="MGRPFNPGYILEVLPRLLAFLPVTLGVAAATAVLGTLLGFVLAK
                     AKLGSSALARGLADAYTWVLRCTPSIVLLFLVYYGLPALVKAAFGVDASRWHRAFYVV
                     LTFTLFFAATMSELMRSAYLAVDRGQREAALSHGLSEFQAFRRIVLPQAAVSALPNVA
                     TSLVALLKEGSLAYTVGLVDLMGQGTLIIA"
BASE COUNT          362 a          732 c          682 g          352 t
ORIGIN      
        1 catagcctac cacgaggccg gccacgccat cgtcgccgcg ttcaccccgg aggccgaccc
       61 cgtgcagaaa gtgtccatcg tgcctagagg cttcggcgcc ctcggcttca cgctgcaggt
      121 accgatcgag gaccggtata tcgtcacgga ggacaagctc ctgggcgaga tcgacgtcct
      181 gatgggcggc cgcgccgccg aggaactgac cttcggcaag gtgtcgacgg gcgctgcgaa
      241 cgacatcacc agggcgaccg acatagctcg ccgcatgata accgactacg gcatgtcgga
      301 acgctaccgc aacgtggcgc tgaccaagcg cggcgcaggc atgatggggc cgtcggccga
      361 gccggtgatg gccagagagt acggcgagga gacgcaacgc tacatcgacg agacggtcgc
      421 cgcgacggtc gccgcgcgct acgagatcgc tcggcgcacc ctcgacgagc gccgcggcct
      481 cctggacacg ctggccgggg agctactcga gaaggagtcg atcgacgagg cggccttcaa
      541 ggccatcgtc gcgccgcccc gggcgagcga cgagaggctg gttagctcct agggttccta
      601 tcggctagac aaacgataca gtttgcgata cacttcagtg tgcatgcgta cccgatgtag
      661 ccgatcgggc cgcatactgg aggataccca tgaaatcgat ccacgatagc gcgtcgctag
      721 gcttagcggc gctcgtcgcc gcggcggccc tggccctcgt ctcgtcctgc gacaggccgc
      781 gaggcgacca ggccgcggac gaggccccgc gcgtcgtcta ttcggcccac acccagaact
      841 atgtgcccta cggttacatc gacgagaagg gcggttccga cggcttcgag atcgccgtac
      901 tcagggcggt cgacgagctc ctgcccgact acgaattccg cttcgtgccg acctcggacg
      961 aggacctgtt cattggcctc gagacgggcc gctaccagct cgccaccaag ggcgcctggg
     1021 cgaccgcgga gcgccgcgag aagttcgtct tcccgaagaa ccccatagcc gcgagcgtca
     1081 tcggcatagc cttcaggagc gaggactcag gacagatcaa ggacctggac agcttcgccg
     1141 cctactcggg taagctcgtg ccgatctcgc cgcagagcgc ccagtatacc gtcgtttccg
     1201 actataacgc ggcccatccg gagcatccga tcaagctcgt gccgtcggat gtgttcatca
     1261 tcaacgacgc ctacctctgg gtgctcgagg gccgctacga cgccttcttc gacatcaagc
     1321 tatcgttcca gaagaacgtc gaggccgaag acggcccctt ccaccacctg gcctcgcggc
     1381 tagcctatgc gccgtataag ggcatcccga cctggccgct gttcgccagg ggcgagcaag
     1441 ccctggccga cgcctacgac gaggccatcg agagactcaa ggccgagggc acgctggcgg
     1501 agctgtcaag gcgttacttc ggcgaggacg tcttccgctt cgcggagtag gcgcaccgtg
     1561 ggacgcccgt tcaacccggg atacatcctc gaggtactgc ctcggctgct cgccttcttg
     1621 ccggtgacgc taggcgtggc ggcggccacg gcggtactcg ggacgctgct cggcttcgtg
     1681 ttggccaagg ccaagctcgg aagctcggcc ctggccaggg gtctggctga cgcgtacacc
     1741 tgggtcctgc gttgcacgcc gtcgatcgtg ctcctattcc tcgtctacta cggcctaccg
     1801 gccctcgtta aggcggcctt cggcgtcgac gccagccggt ggcaccgcgc cttctacgtc
     1861 gtgctcacct ttacgctgtt cttcgcggcg acgatgtccg agctgatgcg ctcggcctac
     1921 ctcgcggtcg accggggcca gcgcgaggcg gccctgagcc acggcctgag cgagttccag
     1981 gccttccgcc gcatcgtgct gccgcaggcg gccgtgtcgg ccctgccgaa cgtcgccacc
     2041 tcgctggtcg ccctgctcaa ggaaggctcg ctcgcctaca ccgtcggcct ggtcgacctg
     2101 atgggccagg gcacgctgat catagcgc
//
LOCUS       DAOOLN010000251         2123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_65414_length_2123_cov_2.335590, whole genome shotgun sequence.
ACCESSION   DAOOLN010000251 DAOOLN010000000
VERSION     DAOOLN010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65414_length_2123_cov_2.335590"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..1555)
                     /locus_tag="PLW80_04070"
     CDS             complement(185..1555)
                     /locus_tag="PLW80_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB65712.1"
                     /translation="MSFVIILIVFFGIAVGVLSFFLIRLIVMPKRLSAVAELIKQGRY
                     QAATKAVKSMLAKEPRNAAAHYYLGKIYLAEGKPELALMEFKTVGDIGQFGHEIPETE
                     FRRLIADLYERFGQLEEALKEYILLAKGDPRNAEYFYQCSRIFDERGKADVATKYARK
                     AVELDPRHGKAHYMLGVILYKTKHPLEAKAEFELGLKFDPSNYDSYYYLGKLMKESND
                     YTGALLAFERAQKSPALKIKALVERGGTYMSQGSYDNAAIELERAVKLAKDDGSSESL
                     YGRYFLSLCYEKMKNIDKAIEQWEKIYAKKPQFRDVAEKLTQYQEYRTDDRIKDYLTC
                     GQDDFIELCKLTAMNGLNLAVRDVIPTPNGVDFITVENESDKWLGTKKMPRLLRFLRV
                     SEQLDDSSIRSLLDNMKKLGIIRGVIVTSSGFNRSAIEFAENRSVELYHKEQLQELLK
                     KASASS"
     gene            1727..>2123
                     /locus_tag="PLW80_04075"
     CDS             1727..>2123
                     /locus_tag="PLW80_04075"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB65713.1"
                     /translation="MLRRGFYRRATPGRFVARLALAFALGTAAVGASELSDRGIGLFM
                     ENKPQEAAPVLELAAKEAATDEKVFLYLGIAYQQLGRWDDAIAAFRRGLAASALYRHQ
                     FLFNIANSFFAQGKNAFALVYGSSQQPDGL"
BASE COUNT          378 a          632 c          657 g          456 t
ORIGIN      
        1 acgctaaaat atcgtaaagc gcggtcaagt gtcaaccgag cccgtcatcc tataagaccc
       61 ccggaccctg gtccggatcc gggggcgcac cacggaaagg aggaacaccg gccgatcgac
      121 gatcgatcga tgccaaccta acaacaaccg gtggcggcgg cggttacggc cgttcgatcc
      181 gaggctacga ggaagcgctg gccttcttaa gtagctcctg tagctgttcc ttgtggtata
      241 actcgaccga tcggttctcg gcgaattcaa tcgccgagcg gttgaagccg gagctcgtga
      301 cgatcacccc gcggatgatg cctagcttct tcatattgtc gaggagcgag cggatcgacg
      361 agtcgtcgag ctgttccgag acgcgcagga agcgtaacag tcgcggcatc ttcttcgtgc
      421 cgagccactt atcagactcg ttctcgaccg tgatgaagtc gacgccgttc ggcgtcggga
      481 tcacgtcgcg cacggcgagg ttgaggccgt tcatcgcggt aagcttgcac agttcgatga
      541 agtcgtcctg gccgcaggtc aggtagtcct tgatgcggtc gtcggtgcgg tattcctggt
      601 actgggtcag tttctcggcg acgtccctaa attgaggctt cttggcgtag atcttctccc
      661 actgctcgat agccttgtcg atgttcttca tcttctcgta gcacagcgac aggaagtagc
      721 gcccgtagag ggattcggac gagccgtcgt ccttggccag cttgaccgcc cgctcgagct
      781 cgatggcggc gttatcgtag gagccctggc tcatgtaggt accgcctcgc tcgacgaggg
      841 ccttaatctt gagggccggg ctcttctgcg cccgctcgaa ggcgagcagt gcgccggtgt
      901 agtcgttcga ttccttcatc agcttgccga ggtaatagta gctgtcgtag ttggacgggt
      961 cgaattttag gcccagctcg aactccgcct tggcctcgag tgggtgcttg gtcttgtaaa
     1021 ggatgacgcc gagcatgtag tgggccttgc catgacgggg atcgagctcc accgccttgc
     1081 gtgcgtactt cgtggccaca tcggccttgc cgcgctcgtc gaagatcctg gaacactggt
     1141 agaagtactc cgcgttccgc gggtctccct tggcgagcag gatgtactcc ttgagcgcct
     1201 cttcgagctg gccgaagcgc tcgtacaggt cggctatcag ccgtctgaac tcggtctcgg
     1261 gaatctcatg gccgaactgg ccgatgtcgc cgaccgtctt gaactccatc agcgcgagct
     1321 ccggcttgcc ttcggccagg tagatcttgc ccaggtagta gtgggcggcg gcgttcctcg
     1381 gttccttggc tagcatggac ttgacggcct tggtggccgc ctgatagcgg ccctgcttga
     1441 tcaattcggc caccgccgat agccgcttgg gcatgacgat caggcggatg aggaaaaagc
     1501 tcaggacgcc taccgcaatg ccgaagaata ctatgaggat gatgacgaat gacatcggcc
     1561 cgccttgctt ctctctatat tccggatggc ccggtcgcgc tttaaacgaa gcgcctcccg
     1621 tgatatccta ataaaaggta tacgatcttg tccatctatt ctatagtagc gccgataccg
     1681 gacgcgcgca acggcggggc gtcggctcgc tagacgaggg ggtgcgatgc tcaggcgagg
     1741 attctatcgg cgcgcgacgc ccggccgctt cgtcgcgcgc ctggcgttgg ctttcgcgct
     1801 cggcacggcc gccgtcggag cctcggagct gtcggatcgc ggtatcggcc tgttcatgga
     1861 gaataaaccg caggaagccg cgcccgtgct cgaactggcc gccaaggaag cggcgactga
     1921 cgagaaggtg ttcctgtacc tcggcatagc ctaccagcag ctcggccgtt gggacgacgc
     1981 catcgcggcg ttccgtaggg ggctcgccgc ctcggcgctg taccgtcacc agttcctgtt
     2041 caatatagcc aactcgttct tcgcccaggg caagaacgcc ttcgccctgg tctacggcag
     2101 cagccagcag cccgacggat tgt
//
LOCUS       DAOOLN010000252         2118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_65645_length_2118_cov_2.885603, whole genome shotgun sequence.
ACCESSION   DAOOLN010000252 DAOOLN010000000
VERSION     DAOOLN010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65645_length_2118_cov_2.885603"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PLW80_04080"
     CDS             complement(<1..199)
                     /locus_tag="PLW80_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65714.1"
                     /translation="MSTRAITRYATLAAVSMACLLTAAAPAAGQAAASADAATAAITQ
                     IDSARLLARQGVRLYVAGIKGG"
     gene            complement(226..1119)
                     /locus_tag="PLW80_04085"
     CDS             complement(226..1119)
                     /locus_tag="PLW80_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPB65715.1"
                     /translation="MSLTLKRLFMALFGLLGALVAWPALSALRFLQPRFPGYLVFTLA
                     QGAAAGLAFGAMLGSIEGVVVSSRAKALRGLGFGALYGALAGAAGAAAGQLAMFVAGE
                     TLFRSASARLGPALALSNGVAWAIIGLAIALTEGLRARSPRKLAAGLAGGTVDGLLGG
                     AVLSALSYARPDDGAALLAGLAIFGLAIGASYAVFENRFSFGALKLLNGPLKNKEYLL
                     ASRRLTIGSDDACDVVLKGYPGVAPLHAVIRAGKDGSRLEPGPDSPRVAVNDEPLGDR
                     PLRLEDVIAIGKAKFIYGYFG"
     gene            complement(1122..>2118)
                     /locus_tag="PLW80_04090"
     CDS             complement(1122..>2118)
                     /locus_tag="PLW80_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65716.1"
                     /translation="GKKPFPGSFSPETVNLIQKGRYRKPSSVNRSVKPIVDRLVRKLA
                     RPDPKRRYQDMSEVIRVVERFLARYQAAELDEALVGLMTGSLKDEPRYRPRRKKRLAW
                     ATVLLALAAACAGGWYAYREGYAYRWLIPSRYGELRVSLRVPKAGLSPDGPSVRARLF
                     VDDGDEMPEVTGAAIVFGLSEPAASDPALAFESGPLYLAPGDYRLKLIVDGRVLWRSF
                     RLRPISLEPSTALRLRLDEEPAKPLSVRVAASDLTDGSDLSGRATVSLYRFGYWVPLS
                     ELPAGTLMTGSVHKFQIEAEGYEPSVYSLSIGNGQDELRIEAALAPFGAAPSAKE"
BASE COUNT          345 a          725 c          743 g          305 t
ORIGIN      
        1 cgccgccctt gatcccggcg acatacagcc tgacgccctg cctggcgagg agcctggcgc
       61 tgtctatctg cgtaatcgcg gcggtagccg cgtcggcgct agccgcggct tgccccgcgg
      121 cgggcgcggc ggcggtcagc aggcaggcca tcgaaacggc cgccagcgtc gcgtatcggg
      181 taatcgcgcg cgtactcatg cttcgctcct tatcgtcggc acgcttcaac cgaagtatcc
      241 gtagatgaat ttggccttgc cgatcgctat cacgtcttcc aggcgtagag gacggtcgcc
      301 gaggggctcg tcgttgacgg cgacgcgcgg ggagtccggc cccggttcga ggcgtgagcc
      361 gtccttgccg gcgcggatca ccgcgtggag cggcgcgact cccggatagc ccttgagcac
      421 gacgtcgcac gcgtcgtccg agccaatcgt cagccgtcgg ctcgcgagca ggtattcctt
      481 gttcttgagg gggccgttga gcagcttgag cgccccgaag gagaaacggt tctcaaagac
      541 ggcgtaggac gcgcctatgg cgaggccgaa gatagccagc ccggcgagca gggcggctcc
      601 gtcatcgggg cgcgcgtacg atagcgccga taggacggcc ccgccgagta gcccatccac
      661 agtgccgccg gcgagtccgg cggccagttt gcggggagat cgggcgcgca ggccctcggt
      721 cagcgctatg gccaggccga tgatggccca ggccacgccg ttcgacagcg ccaaggccgg
      781 cccgagcctg gcggaggccg acctgaatag tgtctcgccg gcaacgaaca tggccaactg
      841 cccggcggcg gctcccgcgg cgccggcgag ggcgccgtat agggccccga acccgagtcc
      901 ccgcagcgcc ttggcgcgcg acgagacgac gacgccctcg atcgagccga gcatcgcgcc
      961 gaaagccaga ccggctgcgg cgccctgcgc caacgtgaaa accaggtagc cggggaagcg
     1021 cggctgtagg aacctgagcg ccgacagggc cggccaggcg accagcgcgc cgagtaggcc
     1081 gaacagggcc atgaagagtc gcttaagcgt cagggacatg atcactcctt ggcggacggc
     1141 gccgctccga acggggccag ggccgcctcg atgcgtagct cgtcctgccc gttgcctatc
     1201 gatagcgagt agaccgacgg ttcgtagccc tcggcctcta tctggaattt atgtaccgat
     1261 ccggtcatca gcgtgccggc cggcagctcc gacagcggga cccagtagcc gaaacggtac
     1321 agcgataccg tcgcccgtcc gctcaggtcg gagccgtccg tcaggtcgct cgccgcgacc
     1381 ctgacggata gcggcttggc cggctcctcg tcgagcctga gcctgagcgc cgtcgacggc
     1441 tctaaggata tcggcctcag cctgaacgag cgccagagca cccgcccgtc gacgatcagc
     1501 ttgaggcggt agtcgcccgg agccaggtac aacggccccg actcgaaggc tagggccggg
     1561 tctgacgccg ccggctcgga gaggccgaag acgatggccg cgccggtcac ttcgggcatc
     1621 tcgtcgccgt cgtcgacgaa caggcgcgcc cgcaccgacg ggccgtcggg cgagaggccg
     1681 gccttcggga cgcgcagcga gacgcgcaac tcgccgtacc gcgacggaat aagccagcgg
     1741 taggcgtagc cctcgcggta ggcgtaccag cccccggcgc aggccgcggc cagcgctagg
     1801 aggaccgtcg cccaggcgag ccgcttcttg cgccgcggcc ggtaacgggg ctcatccttc
     1861 agcgaaccgg tcatcagccc gacgagcgcc tcgtcgagct cggccgcctg gtagcgtgcc
     1921 aggaagcgct ccaccacgcg tatgacctcg gacatgtcct ggtagcgccg cttgggatcg
     1981 ggcctggcca gcttgcgcac caggcggtcg acgatcggct tgaccgagcg attgacggag
     2041 ctcggcttac ggtagcggcc tttctggatc aggttgaccg tctcggggct gaagctaccg
     2101 gggaagggct tcttgccg
//
LOCUS       DAOOLN010000253         2114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_65828_length_2114_cov_2.888295, whole genome shotgun sequence.
ACCESSION   DAOOLN010000253 DAOOLN010000000
VERSION     DAOOLN010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65828_length_2114_cov_2.888295"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1798)
                     /locus_tag="PLW80_04095"
     CDS             complement(<1..1798)
                     /locus_tag="PLW80_04095"
                     /inference="COORDINATES: protein motif:HMM:NF013384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HPB65717.1"
                     /translation="MPGRWFASDNNAAAHPRVMEALVRANDGHAVGYGDDPLTATAEA
                     AVGSLFGPGAVVRFVLNGTGANVYALGCFAGQGDAILCSDCAHILVDETGAPTSVLGS
                     QLVPLETRDGKIVAGSLVETIRLYDDMHKPRPAALSLSQPTEVGTVYSPAEIAELARV
                     AHGAGLAVHVDGARLSNAAAALGLGPAEASGYRTADGSRGIDAVCFGGTKNGLMFGEA
                     VIFSPAADLPDTARLRKTRLQLASKMRYIAAQFDEYLRDGLWRDCAEAANRRAAALAA
                     GLVDRGYPPEYPVETNGVFVRLPPAVVETLRKKRFFYDWEGGAVRWMASWDTSDDDVA
                     GMLADLDEALSGAGLRGAGQPSRSGAGAGAIIEPPEAMAEKAAAMAEGRRLLLSNWDR
                     VDALRSPESLGLPAPPFVIPAPDGAALVALPDPLASGLGGRSFADCQASRRSRRKYLP
                     EPLSLDELSFMVWSCAGVKSIKNGNAFRVVPSGGCRHPLDLIVYARRVEGLAPGLYRH
                     MATDRSLALLRPASVVPGADPERNGFLSLDGEMDAALSGQLWNCAAAFVWTAVPYRTE
                     WRYSVAAAKTLLLDAGHACQALYGACEALGLGA"
BASE COUNT          319 a          739 c          754 g          302 t
ORIGIN      
        1 aggccccgag gcccagggcc tcgcaggcgc cgtatagggc ctggcacgcg tggccggcgt
       61 cgaggagcag tgtcttggcc gccgcgacgg aatagcgcca ctcggtgcgg tacggcaccg
      121 ccgtccagac gaaggccgcg gcgcagttcc atagctggcc tgatagggcc gcgtccatct
      181 ccccgtcgag cgacaggaag ccgttcctct ccggatcagc tcccggcacg accgacgccg
      241 gccgaaggag cgccagcgat cggtcggtcg ccatgtggcg gtacaggccg ggcgccagcc
      301 cttcgacgcg tcgcgcgtag acgatcaggt cgaggggatg cctgcagccg ccggacggca
      361 cgacgcggaa ggcgttgccg ttcttgatag acttgacgcc ggcgcaggac cagaccatga
      421 acgacagctc gtcgaggctc aggggctccg ggaggtattt gcgcctcgag cggcgcgagg
      481 cttggcagtc ggcgaagctc cgtccgccga ggccggatgc gagggggtcg ggcagggcga
      541 cgagggcggc gccgtcgggc gccgggatga cgaagggcgg cgccggcagg ccgagcgatt
      601 ccgggctcct gagcgcgtcc accctgtccc agttggataa gaggagcctc cgcccctcgg
      661 ccatcgcggc ggctttctca gccatcgcct cgggcggctc gatgatcgcg ccggcgcccg
      721 cgcccgagcg agacggctga ccggcgcccc ggaggcccgc gccggagagc gcctcgtcga
      781 ggtcggccag catgccggcg acgtcatcgt cagacgtgtc ccagctggcc atccagcgca
      841 ccgcgccgcc ctcccagtcg tagaagaagc gcttcttccg gagcgtctcg acgacggccg
      901 gcggcaggcg tacgaagacg ccgttcgtct cgaccgggta ctcgggcgga tagccgcggt
      961 cgacgaggcc ggcggcgagg gccgctgccc ggcggttagc ggcctccgcg cagtcgcgcc
     1021 acaggccgtc cctgaggtac tcgtcgaact gagccgctat gtatcgcatc ttgctcgcga
     1081 gctgaagcct cgtcttccgg aggcgggccg tgtcgggcag gtcggccgcc ggcgagaaga
     1141 tcaccgcctc gccgaacatc aggccgtttt tggtgccgcc gaagcagaca gcgtcgatcc
     1201 cgcgcgagcc gtccgccgta cggtacccgg acgcctcggc cggcccgagc ccgagcgcgg
     1261 cggcggcgtt cgacagcctg gcgccgtcga cgtgaactgc cagccccgcg ccgtgcgcga
     1321 cgcgggcaag ctcagcgatc tcagccggag agtatacggt gccgacctcg gtcggctggc
     1381 tcagggacag cgccgcgggc ctcggcttgt gcatatcgtc gtacaggcgt atcgtctcga
     1441 cgagggagcc ggcgactatc ttgccgtctc tcgtctccag cggcacgagc tgcgagccga
     1501 gcaccgaggt cggcgccccg gtctcgtcga ccaggatatg ggcgcagtcg gaacagagga
     1561 tagcgtcgcc ctggccggcg aagcagccga gcgcgtagac gttcgccccg gtgccgttga
     1621 gcacgaagcg caccacggcg cccggcccga acagcgagcc gaccgccgcc tcggccgtag
     1681 ccgtgagcgg gtcgtcgccg tagccgaccg cgtgcccgtc gttggcgcgt acgagcgcct
     1741 ccatcacgcg cggatgcgcc gccgcgttat tatcgctcgc gaaccacctg cctggcatat
     1801 cccctccgaa tccgaccgaa tcgcgcgcct gcgcggctcc tagagtaggg acagaagccg
     1861 cgctcgtcaa gaatccgtac gacgcgccgc cctgggccgg gttcgtgaat gtatacaggc
     1921 cacgtttatc atctcggcag ggcctgtcga cgagccgtga ccctcgcaaa cgcgagggcc
     1981 gacggctcgt cgacacccgc ctccgcgtgc atgcgaggcc tgggtacgag caaggttttc
     2041 agggcgtcgc gtcgtgccgg tagcgggtag tgctcaagcg ccggttttcg tttcggtcga
     2101 agcaatcacg gcat
//
LOCUS       DAOOLN010000254         2108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_66086_length_2108_cov_3.085241, whole genome shotgun sequence.
ACCESSION   DAOOLN010000254 DAOOLN010000000
VERSION     DAOOLN010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66086_length_2108_cov_3.085241"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /gene="feoB"
                     /locus_tag="PLW80_04100"
     CDS             <1..1255
                     /gene="feoB"
                     /locus_tag="PLW80_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPB65718.1"
                     /translation="LPIFVGVLWSVFQITFALGEYPMAWFESLFGWLGNAAASVIPEG
                     FLRSLVVDGVIGGVGGVFSFVPLIVILFFLLSLLEDIGYMSRAAFATDKILHTFGLHG
                     QSVFPMMLGFGCSVPAIMAARTLKSPRDRIVTILVIPFMSCGAKLPVHVLLAAAFFPN
                     NAGNMVMLIYAIGVLLALGSAWLLKKTVLRGEATPFVMELPPYRAPTVRGVLWHVWEK
                     TWHYIQKAGTIILASSVLIWAVTTFPSYEPTDSELASAEATFLAGSPGATDDEVAARL
                     ETVTAELALANSAAGTFGRFIEPVFRPLGFDWKLGVATVTGFAAKEVVVSTLGVLYSV
                     GTEESEESEGLREALRNDPNWTPLLAFVMMLFTLVIPPCFAALAAIKAELGWKWLGFA
                     FAFMLGLGWALGAAIYQIGSLAGLA"
     gene            1252..1452
                     /locus_tag="PLW80_04105"
     CDS             1252..1452
                     /locus_tag="PLW80_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65719.1"
                     /translation="MSAVIQGAAVVLIVAAAAAYVLRNAVRALKGERRSCCDSGGAEP
                     AGGSSRPPCASCAGCSGCSMKR"
     gene            complement(1536..>2108)
                     /locus_tag="PLW80_04110"
     CDS             complement(1536..>2108)
                     /locus_tag="PLW80_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPB65720.1"
                     /translation="AIAARTNLLAMNAAIEAAHAGEAGAGFAVVAAEIRSLAEQSQLR
                     SKEIAGSVAGIRGGIDKVVASTVDAERAFENIVGHVRKVGELESEIKAAVAEQGSGSR
                     VVLESLSSIRDITDEVRGASAEMTQGAAAAGEEMRRLLELTEELKRSMQDIGKESESI
                     KGVTAKVSALGVRNAELVGQVESGTNRFKV"
BASE COUNT          296 a          749 c          671 g          392 t
ORIGIN      
        1 gctgccgatc ttcgtcggcg tactctggtc cgtattccag atcaccttcg cgctcggcga
       61 gtaccccatg gcctggttcg aatcgctgtt cggctggctc gggaacgcgg ccgcgtcggt
      121 catccccgaa ggattcctcc ggtcgctcgt ggtcgacggc gtcataggcg gcgtcggcgg
      181 cgtcttctcg ttcgtgcctc tcatcgtcat cctgtttttc ctgttatccc tgctcgagga
      241 catcggctac atgtccaggg ccgcgttcgc gaccgacaag atcctccata ccttcgggct
      301 gcacggccag tcggtcttcc cgatgatgct cggcttcggc tgctcggtgc cggccatcat
      361 ggcggcccgc acgctcaaga gcccgcggga ccgcatcgtc accatcctcg tgataccctt
      421 catgagttgc ggcgccaagc taccggtgca cgtcctgctc gccgccgcct tcttcccgaa
      481 caacgccggc aacatggtca tgctgatcta cgccatcggc gtgctcctgg ccctcggctc
      541 cgcctggctg ctcaaaaaga ccgtcctgcg cggcgaggcg acgcccttcg tgatggaact
      601 gccgccctac cgggccccga ccgtgcgcgg cgtgctctgg cacgtctggg agaagacctg
      661 gcactatatc cagaaggccg gcaccatcat cctggcctcg tcggtgctca tctgggcggt
      721 cacgaccttc ccgtcctacg agccgaccga ttccgagctg gcgagcgccg aggcgacctt
      781 ccttgccgga agtcccggcg ccaccgacga cgaggtggcg gcgaggctcg agaccgtcac
      841 ggcggagctc gccctggcca acagcgccgc cggtaccttc ggccgtttca tcgaaccggt
      901 cttcaggcca ctcggattcg actggaagct cggcgtcgcc accgttaccg gcttcgcggc
      961 caaggaggtc gtcgtctcga ccctcggcgt gctgtacagc gtcggtaccg aggaaagcga
     1021 ggagagcgag ggactgcggg aagccctgag gaacgacccg aactggacgc cgctcctggc
     1081 cttcgtgatg atgctcttca cgctggtgat accgccgtgc ttcgcggcgc tcgccgcgat
     1141 caaagccgag ctaggctgga agtggctcgg cttcgccttc gcgttcatgc tcggcctcgg
     1201 ctgggcgctc ggggccgcta tctaccagat cggctcgctg gcggggttgg cgtgagcgcc
     1261 gtcatacaag gcgcggcggt cgtactgatc gtggcggcgg ccgccgccta cgtcctgcgg
     1321 aacgccgtcc gggcgctcaa gggcgagcgc cgttcatgtt gcgattcggg cggcgcggaa
     1381 ccagccggcg gttcgtcgcg ccccccctgc gcgagctgcg cgggttgctc cggctgctct
     1441 atgaagcgtt agtggcgaaa cgatgtaaga accgtaccaa gccagcgacg gcggccgcgc
     1501 gagcggtcgc cgttttcggc tgatcgggac ggttcttaca ccttgaagcg gttggtaccc
     1561 gactccacct gacctaccag ctccgcgttg cgcacgccga gcgcggacac cttcgcggtc
     1621 acgcccttga tgctctcgga ctccttgccg atatcctgca tgcttcgttt cagctcctcg
     1681 gtcagctcga gcaggcgccg catctcctcg ccggcggcgg cggcgccctg ggtcatctca
     1741 gcggaggccc cgcgcacctc gtcggttata tcgcggatcg acgaaagcga ctcgagcacc
     1801 acgcgggagc cggagccctg ctcggccacg gcggccttga tctccgactc gagctcgccg
     1861 accttgcgca cgtggccgac gatgttctcg aacgcccgct ccgcatcgac cgtggaagcg
     1921 accaccttgt cgatcccgcc cctgatgcca gccacactac cggctatctc cttggaacgg
     1981 agctgcgact gctcggccag ggaccggatc tccgcggcga cgacggcgaa cccggcgccc
     2041 gcctcgccgg cgtgcgccgc ctcgatggcg gcgttcatcg cgagcaggtt ggtcctggcg
     2101 gctatggc
//
LOCUS       DAOOLN010000255         2107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_66139_length_2107_cov_2.378168, whole genome shotgun sequence.
ACCESSION   DAOOLN010000255 DAOOLN010000000
VERSION     DAOOLN010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66139_length_2107_cov_2.378168"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2107)
                     /locus_tag="PLW80_04115"
     CDS             complement(<1..>2107)
                     /locus_tag="PLW80_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPB65721.1"
                     /translation="DHAVLKGTGVPTVCPIDAECKFTPEVSDYAGRFVKDCDKDIMER
                     LKAEGKLAKRDQILHAYPHCWRCDHPLIYRAVSSWFVKIDPIKPKFVATNQQIHWEPE
                     HIKNGRFGKWLDGARDWAISRSRYWGNPLPIWKCPDCGKTICIGSRDELEKRSGVRLS
                     DLHKHFVDEVTMPCSCGATMSRIPEVLDCWFESGSMPYAQVHYPFENKEHFESHFPAD
                     FISESLDQTRGWFYSLTVLAAALFDRPAYQNCIVSGMVLAADGKKMSKSQRNYTDPME
                     AIDKFGADALRLYLMASPVLKADDLCFTDDGVRDVVKSILLPLWSAYGFFVTYANIDG
                     IEPGEAPIAAANPMDRWILSKCETMVRDVGAGLEAYTMQAAIAPMLDFIDALNNWYIR
                     RSRRRFWRSENDGDKVEAYSTLYRALKRLALCMAPFTPFITETMWRNLRREGEPESIH
                     LAPYPEYDDRYRDEDLEWKMAAVRKAVTMGRSLRYQHNLKIRQPLAAVHLVTKDARER
                     AVLLEMEDIVRDELNVKEVVFREDEEELVEYQAKANFRALGKELGKDMKEGAALVERL
                     EPRQIASLLSGSSLVVDIAGRQVELTRDKVDVRRVEKASLRVVNEGTLTVALDAEITQ
                     ALLDEGNARDLVRGVQTLRKDSGLSVTDRIELSVHGGEALRRSFEAFGDFVSGETLAV
                     ALRWVETPGMTEVEAGDET"
BASE COUNT          320 a          660 c          724 g          403 t
ORIGIN      
        1 caagtctcgt cgccggcctc gacctcggtc atgccaggcg tttcgaccca gcgaagcgcg
       61 acggcgagcg tctcgccgga gacgaagtcg ccgaaggcct cgaacgagcg cctgagcgcc
      121 tcgccgccgt ggacggacag ctcgatgcgg tcggtgaccg acaaaccgga gtccttcctg
      181 agcgtctgga cgccgcggac gaggtcgcgg gcgttgccct cgtcgagcag ggcctgggtg
      241 atctcggcat cgagggcgac cgtcaacgta ccctcgttga cgacgcgtag gctggccttc
      301 tcgacgcggc gtacgtcgac cttgtcgcgc gtcagctcga cctgcctgcc ggctatgtcg
      361 acgacgaggg aagatccgga gaggaggctg gcgatctgcc tgggctcgag ccgctcgacc
      421 agggcggctc cttccttcat gtccttgccg agctccttgc cgagcgcccg gaagttggcc
      481 ttggcctggt actcgaccag ctcctcctcg tcctcgcgga agacgacctc cttgacgttg
      541 agctcgtcgc ggacgatgtc ctccatctcg aggagcaccg cccgctcgcg ggcgtccttg
      601 gtgacgaggt gcaccgccgc gagcggctgc cgtattttga ggttgtgctg gtagcgcagg
      661 gatcggccca tcgtcacggc cttgcgcacc gcggccatct tccactcgag gtcctcgtcg
      721 cggtagcgat cgtcgtactc ggggtagggg gccaggtgga tgctttccgg ctcgccttcg
      781 cggcgcaggt tgcgccacat ggtctcggtg atgaagggcg tgaacggcgc catgcacagg
      841 gccagacgct tgagcgcgcg gtacagggtc gagtaggcct cgaccttgtc gccgtcgttc
      901 tcggagcgcc agaagcggcg gcgcgagcgg cggatgtacc agttgttcag ggcgtcgatg
      961 aagtcgagca tcggcgcgat ggcggcctgc atggtgtagg cctcgagccc ggccccgacg
     1021 tcgcgcacca tcgtctcgca cttggatagg atccagcggt ccatcgggtt cgcggccgcg
     1081 atcggcgcct cgcctggctc gatgccgtcg atgttcgcgt aggtgacgaa gaagccgtag
     1141 gcgctccata gcggtaggag tatgctcttg accacgtcgc ggacgccgtc gtcggtgaag
     1201 cacaggtcgt cggccttgag cactggcgag gccatcaggt agagcctgag cgcgtccgct
     1261 ccgaacttgt cgatagcttc catcgggtcg gtgtagttgc gctggctctt ggacatcttc
     1321 ttgccgtcgg ccgcgagcac catgccggag acgatgcagt tctggtaggc cgggcggtcg
     1381 aacagggcgg ccgcgagcac ggtcagggag tagaaccagc cgcgcgtctg gtcgaggctc
     1441 tcggagatga agtctgccgg gaagtggctc tcgaaatgct ccttgttctc gaaggggtag
     1501 tggacctgcg cgtagggcat cgagccggac tcgaaccagc agtcgagcac ttcggggata
     1561 cggctcatcg tcgccccgca ggagcagggc atggtcacct cgtcgacgaa gtgcttgtgc
     1621 aggtcgctca gcctgacgcc ggaacgtttc tccagctcgt cgcgcgagcc gatacagatg
     1681 gtcttgccgc agtcggggca tttccagatc ggtaaggggt tgccccagta gcggctgcgc
     1741 gatatggccc agtcgcgggc gccgtcgagc cacttgccga agcggccgtt cttgatatgc
     1801 tccggctccc agtggatctg ctggttcgtc gctacgaatt ttggctttat cgggtctatc
     1861 ttgacgaacc agctcgacac cgcgcggtag atgagcggat ggtcgcaacg ccagcagtgc
     1921 gggtaggcgt gcaggatctg gtcgcgcttg gccagcttgc cctcggcctt gagccgctcc
     1981 atgatgtcct tgtcgcagtc cttgacgaag cgcccggcgt agtccgatac ctccggcgtg
     2041 aacttgcact cggcgtcaat ggggcagacc gtcggcacgc cggtgccctt gagcaccgca
     2101 tgatcct
//
LOCUS       DAOOLN010000256         2098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_66559_length_2098_cov_2.303965, whole genome shotgun sequence.
ACCESSION   DAOOLN010000256 DAOOLN010000000
VERSION     DAOOLN010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66559_length_2098_cov_2.303965"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLW80_04120"
     CDS             <1..298
                     /locus_tag="PLW80_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002667902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IIA DNA topoisomerase subunit B"
                     /protein_id="HPB65722.1"
                     /translation="LETPIFRVRTKKETRYCYSPKEREEAVGALGSGSEVTRFKGLGE
                     ISPSEFGQFIGDDIRLLSVDVQSLKSVPEVLAFYMGKNTPERRDFIMKNLKNDL"
     gene            306..>2098
                     /locus_tag="PLW80_04125"
     CDS             306..>2098
                     /locus_tag="PLW80_04125"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HPB65723.1"
                     /translation="MAYVKKLFNDNFLYYASYVIKDRAIPELEDGLKPVQRRILHTLF
                     EADDGKFHKVANIVGSCMKYHPHGDASIGDALVVLANKDLFIDKQGNYGNILTGDEAS
                     AARYIECRASAFAKDVFYNPKITEYVESYDGRNKEPVVFPAKIPVIAITGGEGIAVGM
                     STKILPHNPVEVLAAEIACLRGESFKLYPDFPTGGIVDAGEYQDGLGRVRVRSTLDTS
                     DPKRIVIRDLPFGVTTESLIESVEAAAKAGRLKIASISDFTTDKVEIEVKLARGVYSR
                     ETVDALYAFTQCEQSISCNFLVIKDGQPCLMTVTEVIRHHAKRLLAILKKELELQVKE
                     LLDELHARTLERIFIEERVYKAIETKKTQGAVDKAVLDGLAPFADEIKREVTAEDVER
                     LLRIPIRRISLYDIEKARADMERIKAALKDARHHLAHLTEYGIAFLEGLRKRLGPAWE
                     RKTRVASFDRVDAKEVARRDIALRYDPKTGYLGTNVSGGEYRFDVSPYDKILVIRRSG
                     IYSVVPVPERLFVDTELLYCGIADKDELADTVFTFVYADPKNGSPYIKRCKIEAWIMN
                     RDYQLGKEGGSLLYFTNEPKKAIVLRYAPKPR"
BASE COUNT          440 a          722 c          609 g          327 t
ORIGIN      
        1 cctcgagacg ccgatcttcc gcgttcgcac caagaaggag acccgctact gctacagccc
       61 caaggaacgg gaggaggcgg tcggagccct cggctccggc tcggaggtga ccaggttcaa
      121 gggcctcggc gagatcagcc cgagcgagtt cggccagttc ataggcgacg acatacggct
      181 cctgtcggtc gacgtgcagt cgctcaagag cgtgcccgag gtgctcgcct tctacatggg
      241 caagaacacc cccgagcgcc gcgacttcat catgaagaac ctcaagaacg acctgtagga
      301 atcatatggc atacgtgaag aagctgttca acgataattt cctctactac gcgagctacg
      361 tgatcaagga ccgcgccata cccgagctcg aggacggact caagccggtc cagcgacgca
      421 tcctccacac gctgttcgag gcggacgacg gtaagttcca caaggtggcc aacatcgtcg
      481 gctcgtgcat gaagtaccat ccgcacggcg acgcctccat cggcgacgcc ctcgtcgtgc
      541 tggccaacaa ggacctgttc atagacaagc agggcaacta cggcaacatc ctgaccggcg
      601 acgaggcgtc ggcggccagg tacatcgagt gccgcgcgtc ggccttcgcc aaggacgtct
      661 tctacaatcc caagatcacc gagtacgtcg agagctacga cggtcgcaac aaggagcccg
      721 tcgtcttccc ggccaagata cccgtcatcg ccatcaccgg cggcgagggc atcgcggtcg
      781 gcatgagcac gaagatcctg ccgcacaacc ccgtcgaggt gctcgccgcc gagatagcct
      841 gcctgcgcgg cgagtcgttc aagctgtacc ccgacttccc gaccggcggt atcgtcgacg
      901 ccggcgagta ccaggacgga ctcggccggg tgcgcgtacg ctcgaccctg gacacctccg
      961 accccaagcg catcgtcatc cgcgacctgc ccttcggcgt gactacggag agcctgatcg
     1021 aaagcgtcga ggccgcggcc aaggccggcc ggctcaagat agcctcgatc agcgacttca
     1081 ccacggacaa ggtcgagatc gaggtcaagc tggcccgagg cgtctactcg cgcgagaccg
     1141 tcgacgcgtt gtacgccttt acccagtgcg agcagtccat atcgtgcaac ttcctcgtca
     1201 tcaaggacgg ccagccctgc ctgatgaccg tcaccgaggt catacgccac cacgccaagc
     1261 gcctcctggc aatcttgaag aaggagctcg agctccaggt caaggaactg ctcgacgagc
     1321 tccacgcccg caccctcgag cgtatcttca tcgaggagcg cgtctacaag gccatcgaga
     1381 ccaagaagac ccagggcgcc gtcgacaagg ccgtgctcga cggcctcgcc ccgttcgccg
     1441 acgagatcaa acgcgaagtg accgcggagg acgtcgaacg gctcctacgc attccgatac
     1501 gccgcatctc gctgtacgac atcgagaagg ccagggctga catggagcgc atcaaggccg
     1561 ccctcaagga cgcgcgccac cacctggccc acctgaccga gtacggcatc gccttcctcg
     1621 agggcctccg taagcggctc gggccggcct gggagcgcaa gacgcgggtc gcctcgttcg
     1681 accgagtcga cgccaaggag gtggccaggc gcgacatagc cctgcgctac gaccccaaga
     1741 ccggctatct cggcaccaac gtctccggcg gcgagtaccg cttcgacgta tcgccttacg
     1801 ataagatcct cgtcatccgt cgttcgggca tctactccgt cgtgccggta ccggagcgtc
     1861 tcttcgtcga caccgaactc ctgtactgcg gcatcgccga caaggacgag ctggccgaca
     1921 cggtcttcac cttcgtatat gccgatccca agaacggctc gccctatatc aagcgctgca
     1981 agatcgaggc gtggatcatg aaccgcgact accagctcgg caaagagggc ggctcgctcc
     2041 tgtacttcac gaacgagccc aagaaggcca tcgtccttcg ctacgcaccc aagccgcg
//
LOCUS       DAOOLN010000257         2097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_66611_length_2097_cov_2.688051, whole genome shotgun sequence.
ACCESSION   DAOOLN010000257 DAOOLN010000000
VERSION     DAOOLN010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66611_length_2097_cov_2.688051"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PLW80_04130"
     CDS             <1..400
                     /locus_tag="PLW80_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65724.1"
                     /translation="SLEDAYGSSKWKWSFVELWGKLAVVAKNSLDLKKAFLDMDLASP
                     NRMITLSYLAFIKKQLQSTADRYREKYEVSSMKMDDFKQAILFLNALKRIHVVFGERT
                     EAEELKRKIEIWSAKLESDQKKREEEAKKR"
     gene            complement(428..904)
                     /gene="cdd"
                     /locus_tag="PLW80_04135"
     CDS             complement(428..904)
                     /gene="cdd"
                     /locus_tag="PLW80_04135"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPB65725.1"
                     /translation="MVYSIVPFRQYEALRGNGQKEASMERTALYELAAEAATKSYSPY
                     SRFRVGAALLCADGTVVCAANVENRSYGLAICAERSALVSAIAQGKTEFTELVISTPD
                     SDYPVSPCGACRQFISEFVGADFPIRFGCSKDSYVDSTMGELFPHDALHELKDRTE"
     gene            957..2048
                     /locus_tag="PLW80_04140"
     CDS             957..2048
                     /locus_tag="PLW80_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65726.1"
                     /translation="MSEGKGRRKIFTVLVAVVLIAVYFFVFSEPLRPELSAAPRWTID
                     LSASVASAEASAQDRVDASAVSFSTASRFGYFGTDGSIGFVGEATGPVPMSDSSYVSA
                     ELVPGGANYSLRSPVGARVAELDASAPFYSSGRLFSAQADGSGVAAYDGKGNRLWSYT
                     FPCQLSAFSAGEGLAVGGTVDGWLEGVDSGGRRAFSFAPGGSRLQVVLGLGVSRSGNW
                     IAAVTGVDRQRLVVLGRGGADYRVASHRYLDSDYREPVRVVVLDDDRHVLYRRADGIG
                     VWSVDGAVDELLPVKADDFDVSLDAERGLALLVARRGKKAEVVAFRLPATLLGRVPLP
                     DGSEYVRLIGSSVYFGSGDSISRFDFMEE"
BASE COUNT          356 a          660 c          697 g          384 t
ORIGIN      
        1 ctcgctcgag gacgcctacg gttcgtccaa gtggaaatgg tccttcgtcg agctatgggg
       61 aaagctggcg gtcgtggcga agaacagcct ggacctgaag aaggccttcc tcgacatgga
      121 cctcgcgtcg cccaaccgga tgatcacgct gtcctacctg gccttcatca agaagcaatt
      181 gcagtcgact gccgaccgct atcgcgagaa gtatgaggtc tcgtccatga aaatggacga
      241 ttttaagcag gccatcctct tcctgaacgc cctcaagcgt atccacgtcg tgttcggcga
      301 gcggacggag gccgaggagc tgaagcgcaa gatcgagata tggtccgcca agctcgagag
      361 cgaccagaag aagcgcgaag aagaagccaa gaagcgctga tggcccccgc tccgcgctcc
      421 gcgggcatca ctcggttctg tccttgagct cgtgtagcgc gtcgtggggg aagagctccc
      481 ccatcgtcga atcgacatag gagtccttgg agcaaccgaa ccggatcggg aagtcggccc
      541 cgacgaattc gctgatgaac tgcctgcatg caccgcatgg gctgaccgga tagtccgagt
      601 ccggagtgga gatgacgagc tcggtgaatt cggtcttgcc ttgggcgatg gccgatacga
      661 gggcggaacg ctcggcgcag atggccaggc cgtaggaacg gttctccacg ttggcggcgc
      721 agacgacggt accgtcggcg cacagcaggg ccgccccgac cctgaacctc gaataaggcg
      781 agtaggactt ggtagcagcc tcggccgcca gctcgtatag cgcggtacgt tccatagatg
      841 cctctttctg cccattcccg cgtagcgctt cgtattgacg gaatgggacg atactatata
      901 ccatcgacgg ggagaatgcc aatgacgagc atcgaacgat tgccgcggag ggaagcatga
      961 gcgaggggaa ggggcgacgg aagatattca ccgtcctcgt ggcggtcgtg ctgatcgccg
     1021 tctacttctt tgtattctcc gaacccttgc ggccagagct gagcgccgcg ccccgatgga
     1081 cgatagacct ctcggcgtcg gtcgcgtcgg ccgaagcctc ggcccaggac cgcgtcgacg
     1141 cgtcggccgt ctccttctcg accgcgagcc gtttcggcta tttcggtacg gacggatcca
     1201 tcggcttcgt cggcgaggcg acgggaccag tcccgatgtc ggactcctcg tacgtcagcg
     1261 ccgagctcgt tcccggcggc gcgaactact cgctacgctc ccctgtgggg gcccgcgtcg
     1321 ccgaactcga cgcgagcgcc ccgttttact cgtcggggcg gctgttctcc gcgcaggccg
     1381 acggcagcgg cgtcgctgcc tacgacggca agggaaaccg cctatggtcc tacacgtttc
     1441 cgtgccagct gagcgccttc tctgctggcg aaggcctcgc cgtcggcgga accgtcgacg
     1501 gctggctcga gggcgtcgac tcgggcgggc gcagggcttt ctcgttcgca ccggggggct
     1561 cccgtctcca ggtggtgctc gggctcggcg tctccaggtc cggtaattgg atagcggcag
     1621 tcaccggcgt cgaccggcaa cggctcgtgg tgctcgggcg tggaggcgcc gactaccgtg
     1681 tggcgagcca tcgctacctc gactcggatt acagggagcc cgtccgcgtc gtcgtgctcg
     1741 acgacgatag gcacgtgctc taccgccggg ccgacggcat cggcgtatgg tcggtcgatg
     1801 gcgccgtcga cgagctgcta cccgtcaagg ccgacgactt cgacgtgtcg ctggacgcgg
     1861 agcgcggcct ggccctgctc gtcgccaggc gcggcaagaa ggccgaggtc gtcgcgttcc
     1921 gccttccggc gacgttgctc ggccgcgtac ccttgcccga cggcagcgag tacgtcaggc
     1981 tcatcggctc ctcggtgtat ttcggctccg gggactctat atccagattc gacttcatgg
     2041 aggaatagcg tgtcgctacg ggccgttcga gtcgtcgcgg cgggcgccgt cgtcctg
//
LOCUS       DAOOLN010000258         2093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_66781_length_2093_cov_3.074092, whole genome shotgun sequence.
ACCESSION   DAOOLN010000258 DAOOLN010000000
VERSION     DAOOLN010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66781_length_2093_cov_3.074092"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..915
                     /locus_tag="PLW80_04145"
     CDS             127..915
                     /locus_tag="PLW80_04145"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HPB65727.1"
                     /translation="MPFDERTRILLGDDGADRLAAARVCVFGLGGVGAAAAMDLVRAG
                     VGSLVVVDFDAVSESNLNRLYFGYSGEVGRLKTEVFADYAARVNPECHVEAIGRIVHG
                     AEAAGMIPADCGVYLDCIDTLNPKVNLMAALARAGLPFVSAMGTAGRLAPERLKVGRL
                     WDATHCPLASDVRKRLRRLGFGPPGASLAALGDVGVSMPAAFECVWSDEPPVPPAMPP
                     DGSRPGDCPGGVRVRAVQGSGPFVPQAAGHLLASLAARRLLGRR"
     gene            complement(912..1772)
                     /locus_tag="PLW80_04150"
     CDS             complement(912..1772)
                     /locus_tag="PLW80_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB65728.1"
                     /translation="MAFVAFVAFVALPAIVVVAACSSSRIMEETMVQKTVTVLGKSMY
                     YVESGSGPAVVLVHGNTGSSRWWKKAMNLNGSRTIAPDLPNFGRSDALDTADIDLYAD
                     YLAAFIEALGLERPIVVGHSLGGAVVTAMAARRPELTGALVIVDGAAPSGLVTPEAHY
                     SYIELFRTNRDMMRQALAAVTPTMDDPAFLDELTEDAMAMAPHAFSGNARALARFDYT
                     GRAGAYTGPVMVVWGRKDSIITEAMARETAAAYRGSRLLVLDSVGHSVMAEDPELFKR
                     SLLDFIAGAD"
     gene            complement(1802..>2093)
                     /locus_tag="PLW80_04155"
     CDS             complement(1802..>2093)
                     /locus_tag="PLW80_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65729.1"
                     /translation="AVAALPRDYFEGFARLCDAFLAIDLTRDLGRVACPCLVVVGDRD
                     ILKPPRYSRIIADGVPGARYVEVAGAGHALAIEKPDELAGLLLGFLSEAAGP"
BASE COUNT          309 a          746 c          737 g          301 t
ORIGIN      
        1 cgctacgagc ctggtcgacg acaggcgacc tcgccggcgt ggtcgcccgt ctggccacgc
       61 tacgcggaga ggacgagcgg tccgtggcgg ccgccgtcga ggctaacttc agggaggcgt
      121 tcggccatgc ccttcgatga gcgcacgagg atactgctcg gcgacgacgg ggcggaccgg
      181 ctggccgccg cgcgggtctg cgtattcggc ctcggcggcg tcggggccgc cgcggcgatg
      241 gacctggtcc gcgccggcgt cggctcgctc gtcgtcgtcg acttcgacgc ggtgagcgaa
      301 tcgaacctga accgcctgta cttcggctac tccggcgaag tcggccggct caagaccgag
      361 gtcttcgccg actacgcggc gagagtcaac ccggagtgcc acgtcgaggc gataggacgc
      421 atcgtgcacg gcgccgaggc cgcgggcatg atcccggccg actgcggcgt ctatctggac
      481 tgcatcgaca cgctcaaccc caaggtcaac ctgatggccg ccctggcccg cgccggcttg
      541 cccttcgtat cggcgatggg cacggcgggg cgcctggcgc cggagcggct caaggtcggg
      601 cggctctggg acgcgaccca ctgtccgctc gcctccgacg tgcgcaagcg gctgcggcgc
      661 ctgggcttcg gcccgccggg cgcctcgctc gcggcccttg gcgacgtcgg ggtatcgatg
      721 ccggccgcct tcgagtgcgt atggtccgac gagccgccgg tgccgccggc catgccgccc
      781 gacggtagcc gccccggcga ctgcccgggc ggcgtgcgcg tgcgagcggt acagggctcg
      841 gggcccttcg taccccaggc ggccgggcac ctgctcgcca gcctggcggc gcggcggctc
      901 ctcggaaggc gctagtcggc gcccgcgatg aagtcgagga ggctccgctt gaatagctcc
      961 ggatcctccg ccatgaccga atggccgacc gagtcgagta ccagtagcct ggagccccgg
     1021 taggccgcgg cggtctcccg ggccatagcc tccgtgatga tcgaatcctt gcggccccag
     1081 acgaccatga ccgggccggt ataagcgcct gcgcggccgg tatagtcgaa gcgcgccagc
     1141 gcgcgggcgt tacccgagaa cgcgtgtggg gccatcgcca tggcgtcctc cgtcagctcg
     1201 tcgaggaagg ccggatcgtc catcgtcggg gttaccgcgg cgagagcctg gcgcatcatg
     1261 tcgcgattgg tccggaacag ttcgatgtac gagtaatgcg cctccggcgt gacaaggccc
     1321 gacggcgccg ccccgtcgac gatgaccagc gccccggtca gctccggccg gcgggccgcc
     1381 atcgccgtga ccaccgcgcc cccgaggcta tgcccgacga cgatcggccg ctcgagcccg
     1441 agcgcctcga tgaaggccgc caggtagtcc gcgtacaggt cgatgtcggc cgtatcgagc
     1501 gcgtccgacc ggccgaaatt gggcaggtcc ggcgctatgg tgcggctacc attcaggttc
     1561 atcgccttct tccaccagcg gctcgagccg gtgttgccgt gcacgagcac cacggccggg
     1621 cccgagcccg actcgacgta gtacatgctt tttcccagta cggttaccgt cttctgcacc
     1681 atagtctcct ccatgatccg ggacgaggaa caggccgcga ccacgactat cgccggaagc
     1741 gctacgaacg ccacgaacgc gacgaacgcc acggcgatcc aggctcgtcc cgcgaaacgc
     1801 gtcacggccc ggcggcctcg gacaggaatc cgagcagcag gccggccagc tcgtccggct
     1861 tctcgatggc cagcgcgtgg cctgccccgg caacctcgac atagcgggcg cccggcacgc
     1921 cgtcggctat gatcctcgag tagcgcggcg gcttgagtat atcacggtcg ccgaccacga
     1981 cgaggcaggg gcacgcgacg cgcccgaggt cgcgggtcag gtcgatggcc aggaacgcgt
     2041 cgcacagccg tgcgaagccc tcgaagtagt ccctgggcag ggcggcgacc gcg
//
LOCUS       DAOOLN010000259         2093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_66802_length_2093_cov_2.176644, whole genome shotgun sequence.
ACCESSION   DAOOLN010000259 DAOOLN010000000
VERSION     DAOOLN010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66802_length_2093_cov_2.176644"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1620)
                     /locus_tag="PLW80_04160"
     CDS             complement(<1..1620)
                     /locus_tag="PLW80_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB65730.1"
                     /translation="MVKERVLDLANKISNKQRGAKNEIKPTDPEYMILEPIVTEEMAE
                     VALHLGFRTPKSAEDVAPLCGKTVEDTHRLLMELADAGVCFVNVKDGVDKFWYDTWIP
                     GIMEMMVNKYSNVEKYPQIGRAMEAYGRVRGPMTSGVFPVGTGLMRVIPIEKAIMGET
                     RRASYEEISKYLEENDLFTVSNCSCRSTREIMGVGCGHLKEDMCIQMGHAAEYHIRTK
                     RGRQITREEAYEIIKRAEENGLMHQIPNIDGGGKTHAICNCCGCGCLSLRTAEMFHNT
                     NMVRSNYVAKVDAEKCMACGECVENCPVGALKLGQKIHTKKPLPKLKKPDLPEFTDWT
                     PEHWNPDYRTNREVSFATGTSPCKAECPAHIGVQGYVKLASEGKYAEALELIKRENPF
                     PAVCGRVCSKRCEAGCTRNGLDEPVAVDDIKKFIADQDLNAARRFVPNKRHDYSDKKI
                     AIVGAGPAGLSCAYYLALDGYSVTVFEKRAMLGGMLTLGIPSFRLEKDVINAEIDIIR
                     ELGVEFRTGVEVGVDVTLAELALIFGTAETWA"
     gene            complement(1656..>2093)
                     /gene="hypB"
                     /locus_tag="PLW80_04165"
     CDS             complement(1656..>2093)
                     /gene="hypB"
                     /locus_tag="PLW80_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HPB65731.1"
                     /translation="VAESGTRVIQLHSGGMCHLTADMTRQGLEGLGTEGLDLVILENI
                     GNLICTAEVDVGAAKSAMILSVPEGDDKPLKYPMMFERVDVLLINKIDAISFFNFDME
                     AVRERVTAMNPGIKIIPISAKTGEGVAEWADWIRSAVRDWKRA"
BASE COUNT          316 a          670 c          661 g          446 t
ORIGIN      
        1 cgcccacgtc tccgccgtcc cgaagatcag cgccagctcc gccagcgtca cgtcgacacc
       61 gacctcgacg ccggtccgga actccacgcc cagctcgcgg atgatatcga tctccgcgtt
      121 gatgacgtcc ttctccaggc ggaagctcgg gatgcccagc gtcagcatgc cgccgagcat
      181 cgcgcgcttc tcgaacacgg tgaccgagta gccgtccagg gccaggtagt aggcgcagga
      241 caggcccgcg gggccggcgc ccacgatagc tatcttcttg tcgctatagt cgtggcgctt
      301 attcggcacg aagcggcgcg ctgcgttgag gtcttgatcg gctatgaact tcttaatgtc
      361 gtccacggcc accggctcgt ccaggccgtt acgggtgcaa cccgcctcgc agcgcttgga
      421 gcagacgcgg ccgcagacgg ccgggaacgg gttctcgcgc ttgatcagct ccagcgcctc
      481 ggcgtactta ccctcggacg ccaacttgac gtagccttga acaccgatat gcgccgggca
      541 ctcggccttg cagggactcg tgccggtcgc gaaggatacc tcgcggttgg tgcggtagtc
      601 gggattccag tgctccggcg tccagtcggt gaactcgggc aggtccggct tcttgagctt
      661 cggtagcggc ttcttggtat ggatcttctg gccgagcttg agggcgccga cggggcagtt
      721 ctccacgcac tcgccgcagg ccatgcactt ctcggcgtcg accttggcca cgtagttgga
      781 gcgtaccatg ttggtgttgt ggaacatttc agcggtacgc agggatagac agccgcagcc
      841 gcagcagttg cagatggcgt gggtcttgcc tccgccgtcg atgttgggaa tctggtgcat
      901 caggccgttt tcctcggccc gcttgattat ctcgtaggct tcctctcggg tgatctgacg
      961 gccgcgtttc gtgcgtatgt ggtactccgc ggcgtggccc atctggatgc acatgtcttc
     1021 cttcaggtgg ccgcagccca cgcccatgat ctcgcgcgtg gagcggcatg agcagttcga
     1081 cacggtgaag aggtcgttct cctccaggta cttggatatt tcctcgtagg aggcccgcct
     1141 ggtctcgccc atgatggcct tctcgatcgg gatgacgcgc atgaggcccg tgccgacggg
     1201 gaagacgccg gaggtcatgg gcccgcggac gcggccatag gcctccatcg cgcggccgat
     1261 ctgcgggtac ttctcgacgt tggagtactt gtttaccatc atctccatga tgccgggtat
     1321 ccaggtgtcg taccagaact tgtcgacacc gtccttgacg ttgacgaagc acacgcccgc
     1381 gtcggcgagc tccatgagca ggcggtgcgt gtcctccacg gtcttgccgc acagcggcgc
     1441 cacgtcctcg gcgctcttgg gggttcggaa ccccaggtgc agggccacct cggccatctc
     1501 ctcggtgacg atgggctcca ggatcatgta ctccgggtcg gtgggcttga tctcgttctt
     1561 ggcgccgcgc tgcttgttgc tgatcttatt ggcaagatct aggactctct ctttgaccat
     1621 gtgcctctcc tcgtgtgatt ctgcgtagcc cggggctacg cccgcttcca gtcccgtacg
     1681 gcggagcgga tccagtccgc ccactcggcc acgccttcgc cggtcttcgc cgatatagga
     1741 atgatcttga tgccggggtt catcgccgtg acgcgctcgc gtacggcctc catgtcgaaa
     1801 ttaaagaagc tgatcgcgtc tatcttgttg atgaggagga cgtccacccg ctcgaacatc
     1861 atcgggtact tgagcggctt gtcgtcgccc tcgggcaccg acaggatcat cgcgctcttg
     1921 gcggcgccca cgtcgacttc ggccgtgcag atcaggttgc cgatgttctc caggatgacg
     1981 aggtccaggc cctcggtgcc gaggccctcg agaccctgcc tggtcatgtc ggcggtcagg
     2041 tggcacatgc cgcccgagtg aagctggatg acgcgcgtgc cgctctcggc gac
//
LOCUS       DAOOLN010000260         2092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_66833_length_2092_cov_2.933726, whole genome shotgun sequence.
ACCESSION   DAOOLN010000260 DAOOLN010000000
VERSION     DAOOLN010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66833_length_2092_cov_2.933726"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="PLW80_04170"
     CDS             complement(<1..579)
                     /locus_tag="PLW80_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65732.1"
                     /translation="MATLKRYKDFEKSVAKRASASVRRGFSSVFSAAARFFKAGRQKL
                     TIMLVPHSEKRIFNLQISLFGLAAVIALALGAAGFLAYSSVGYGDVVGKLAGKTESLK
                     ATQADLDAIRDSTARLVKSARSFQAALDSTLAGIGIKSQSDAASRADGDLAAFFSPSE
                     AGSGRLREAAEIERVSAYLERSVKPLDELGDTI"
     gene            703..1569
                     /locus_tag="PLW80_04175"
     CDS             703..1569
                     /locus_tag="PLW80_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB65733.1"
                     /translation="MTDASEKSREPDSGKALLSRSDDRLSIFFIGAGSAFTKRYYQNN
                     ALVVKGDDHLLIDCGTRAPEALTRLGLSVTDIDTYLITHSHADHIGGLEEVMLLNRYM
                     AGKRPTMVAPAAYRKYLWKHSLKGGAAYNERREGRFLRFEDLWDAVDLEPVPGADRQL
                     CETSVGSIRLAMFRTRHIPDSAPSWRSCAPSYGVVIDGRVLYTSDTRYDPEMLRFVEE
                     RYDIESVFHDCQLFTGGVHASLDELAGLPPALKAKTLLMHYGDAVDSAEAKAETLGFK
                     GFARQWTPYLFG"
     gene            1545..>2092
                     /locus_tag="PLW80_04180"
     CDS             1545..>2092
                     /locus_tag="PLW80_04180"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPB65734.1"
                     /translation="MDAVPLRLGLVAALASALSIVAVGCAGAPPSGDGYAALLGELGA
                     EPRPFIEAGGAAVRAFVIGDPGAPKRVVFVHGWSGSGAEFVEMAMRLSRARGDVLCIC
                     VDLPGSGSSDKPADASYDVPYFRDVIAEAVEAAATYGLARGQRSTDITLVGHSLGGHL
                     SVAYAASADASIARLALVSPAG"
BASE COUNT          335 a          696 c          711 g          350 t
ORIGIN      
        1 tatcgtgtcg ccgagctcgt cgagcggctt gacgctgcgc tcgaggtagg cgctgacccg
       61 ctctatctcg gcggcctcgc gcaggcggcc ggagccggcc tccgagggcg agaagaacgc
      121 ggccaggtcg ccgtcggcgc gcgaggccgc gtcggactgg ctcttgatgc cgatgccggc
      181 cagcgtcgag tcgagggcgg cctggaagga gcgcgccgac ttcaccagcc tggcggtgct
      241 gtcgcggatg gcgtcgaggt cggcttgggt agccttgagg ctctcggtct tgccggccag
      301 cttgccgacc acgtcgccgt agccgacgga cgaatacgcc aggaagcccg cggcgccgag
      361 cgcgagggcg atcacggcag ctagtccgaa gaggctgatc tgaaggttga agatgcgctt
      421 ctcggagtgg gggaccagca tgatggtaag cttctgccta cccgccttga agaaccgtgc
      481 cgcggcgctg aaaacggatg agaaaccgcg gcggaccgag gcgctcgcgc gcttggccac
      541 cgatttctcg aaatccttgt atcgtttcag cgtcgccaac gagggcctcc ggtctctata
      601 aattcattcg acgatagcac gtcgcggccg tcgctgtcaa accgctggct tccggcgcgc
      661 ctcgttttga aatcgctccg accgtacagt atagtatggt ttatgaccga tgcaagcgaa
      721 aaatcgcgcg aaccggactc gggcaaggcc ttgctgtcga ggtcggacga ccgtctatcc
      781 atattcttta tcggcgccgg cagcgccttc accaagcgct attaccagaa caacgcgctc
      841 gtggtgaagg gcgacgacca tctgctcatc gactgcggca ccagggcgcc cgaggccctc
      901 acgaggctcg gcctatcggt taccgatatc gatacctacc ttataacgca ttcccatgcg
      961 gaccatatag gcgggctcga ggaggtcatg ctgctcaacc gctacatggc cggtaaaagg
     1021 ccgactatgg tagccccggc ggcctaccgg aagtacctct ggaagcactc gctgaagggc
     1081 ggggcggcat acaacgagcg ccgcgagggc cgcttcctgc gcttcgagga cctctgggac
     1141 gcggtcgacc tcgagccggt gccgggcgcg gaccgtcagc tctgcgagac gagcgtgggc
     1201 tccatccggc tggccatgtt caggaccagg cacatccccg actcggcgcc gagctggcgc
     1261 tcctgtgcgc ccagctacgg cgtggtcatc gacggccgcg tgctctacac gagcgacacg
     1321 cgctacgacc ccgagatgct gcgcttcgtc gaggagcgtt acgacatcga gagcgtgttc
     1381 cacgactgcc agctgttcac cggcggcgtg cacgcctcgc tcgacgagct ggccgggttg
     1441 ccgcccgcgc tcaaggccaa gaccctgctg atgcactacg gcgacgccgt cgattcggcg
     1501 gaggccaagg ccgagacgct cggcttcaag ggcttcgcgc gacaatggac gccgtacctc
     1561 ttcggctagg gctcgtagcc gccctcgcct cggcgctgtc gatcgtcgcg gtcggctgcg
     1621 ccggcgctcc gccctccggc gacggctacg ccgccctgct cggcgagcta ggcgccgagc
     1681 cgcggccgtt catcgaagcg ggcggcgcgg cggtccgggc cttcgtgatc ggggacccgg
     1741 gggcgcccaa gcgggtcgtc ttcgtgcacg gctggtccgg tagcggggcg gagttcgtcg
     1801 aaatggccat gaggctaagc cgagctcgag gagacgtcct ttgcatctgc gtcgacctgc
     1861 cgggttcggg ctcctccgac aagccggccg acgcgtccta cgacgtgccg tacttcaggg
     1921 acgtgattgc ggaggcggtc gaggcggccg cgacctacgg cctggccaga gggcagcgct
     1981 cgaccgacat aactctggta ggccacagcc tcggcggcca cctgtccgtc gcctacgccg
     2041 cgtcggcgga cgcgagcatt gccaggctcg cgctcgtctc gccggccggc tg
//
LOCUS       DAOOLN010000261         2088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_67031_length_2088_cov_2.100344, whole genome shotgun sequence.
ACCESSION   DAOOLN010000261 DAOOLN010000000
VERSION     DAOOLN010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67031_length_2088_cov_2.100344"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="PLW80_04185"
     CDS             complement(<1..1167)
                     /locus_tag="PLW80_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB65735.1"
                     /translation="MKDLVFDSVVIGGGAAGMAAALELDRAGFSCAIVEREEALGGIL
                     TQCIHNGFGLIEFNEELSGPEFAERIEERLAGTRITSFTGATVMELDASGDIKTLYCY
                     TGAHGVMRLSARAVVLAMGCRERNRGNIRIPGDRPAGVYTAGLAQRLVNIEGFVPGKD
                     VVIIGSGDIGLIMARRMSWIGCKVHAVVEILPYPSGLTRNIVQCLHDFGIPLYLSHLT
                     TNIVGRERVEGVEITPIENGATRFDKAFTIPCDTVLLSVGLVPENELSKIAGVELNPM
                     TNGPVADSMLMTSVPGVFACGNVLHVHDLVDYVVEEARRAGAHAARWLKGERPKAEAR
                     VKAGPNVRYCAPSRVDLHAANKLYMRTLIVKNDATLEVKVNNRVVKSVKKNHIQP"
     gene            complement(1164..>2088)
                     /locus_tag="PLW80_04190"
     CDS             complement(1164..>2088)
                     /locus_tag="PLW80_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB65736.1"
                     /translation="MTGFKVEHAARAGDEWTVRAADGRVVKARYVVNAAGLYADEVSR
                     AFGAEEFEIKPRKGEYYLLDRLTKAKPERVIFPVPTSVSKGMLVIPTVEGTVLIGPTA
                     EAVDDKADLATTREQLEKILSSARAMVPSVSEYDVITSFAGLRPTLEEDFYIEPSAKA
                     PALIQVAGIQSPGLTASPAIGEYVKDLLKKEGLRLSEKPDYDPFVGKRPHVKDLTPFE
                     VDKLAQADPAYANMICRCEKVSEAEIVQAIRAGHTTLDGIKYFTRAQMGRCQGGFCTY
                     KIIKIIMRETGLSWDEITKNGDGSRILKDAL"
BASE COUNT          319 a          676 c          707 g          386 t
ORIGIN      
        1 cggctggata tggttcttct tgacgctctt gacgacgcgg ttgttcacct tgacctcgag
       61 ggtcgcgtcg ttcttgacga tcagggtcct catgtacagc ttgttggccg cgtgcaggtc
      121 gacgcgggac ggcgcgcagt agcgcacgtt cggccccgcc ttgaccctgg cctcggcttt
      181 gggtcgctcg cccttgagcc agcgcgcggc gtgggcaccg gcccgcctag cctcctcgac
      241 cacgtagtcg accaggtcgt gtacgtgcag cacgttgccg caggcgaaca cgcccggcac
      301 gctggtcatc agcatggagt cggccacggg gccgttcgtc atcggattca gctcgacgcc
      361 ggcaatcttg gacagctcgt tttcgggcac gaggccgacg gacaagagga ccgtgtcgca
      421 gggtatcgtg aaggccttgt cgaagcgcgt ggcgccgttc tcgatcgggg tgatctccac
      481 gccctcgacc cgctcgcgcc cgacgatgtt ggtggtaagg tgcgacaggt acagcggtat
      541 gccgaagtcg tgcaggcact ggacgatgtt gcgcgtcagc ccggacgggt acggcaggat
      601 ctcgacgacg gcgtgcacct tgcagcctat ccagctcatg cgcctggcca tgatcaggcc
      661 gatgtcgccg gagccgatga tgacgacgtc cttgccgggc acgaagccct cgatgttgac
      721 caggcgctgg gccaggccgg cggtataaac gcccgcgggg cggtcgccgg gtatgcggat
      781 gttgcctcgg ttgcgctcgc ggcagcccat agccagcacg acggcgcggg cggagagccg
      841 catcacgcca tgggcgccgg tgtagcagta cagcgtcttg atatcaccgg aggcatccag
      901 ctccatcaca gtggcgccgg tgaacgaggt gatgcgcgtc ccggccaggc gttcctcgat
      961 ccgctcggcg aactcggggc cggacaactc ctcgttgaac tcgatgaggc cgaagccgtt
     1021 gtggatgcac tgcgtcagga tgccgccgag cgcctcctcg cgctcgacga tggcgcagga
     1081 gaagccggcg cggtccagct cgagggcggc ggccatgcct gccgcccctc cgcctatcac
     1141 taccgagtcg aaaacgaggt ctttcataga gcgtccttga gtatgcggct gccgtcgccg
     1201 ttcttggtta tttcgtccca cgagaggccg gtctcgcgca tgatgatctt gatgatctta
     1261 taggtgcaga agccgccctg gcagcggccc atctgcgcgc gcgtgaagta cttgatgccg
     1321 tcgagcgtgg tatgcccagc cctgatggcc tggacgatct ccgcctccga caccttctcg
     1381 cagcgacaga tcatgttggc gtaagccggg tcggcctggg ccagcttgtc gacctcgaag
     1441 ggcgtcagat ccttgacgtg ggggcgcttg ccgacgaagg ggtcgtagtc cggcttctcc
     1501 gacagcctga ggccctcctt cttgagcagg tccttgacgt actcgccgat ggcggggctg
     1561 gcggtcaggc cgggcgactg gatgccggcc acctggatca gggcgggagc cttagcgctc
     1621 ggctcgatat agaaatcctc ctcgagcgtc gggcggagtc ccgcgaagct cgtgatcacg
     1681 tcgtactcgg agaccgacgg caccatggct cgggccgacg acaggatctt ctcgagctgc
     1741 tcgcgggtag tcgccaggtc ggccttgtcg tccacggcct ccgccgtggg gcctatgagc
     1801 accgtgccct cgacggtcgg tatgacgagc atgcccttgg agacgctcgt cggcacgggg
     1861 aagatcacgc gctcgggctt ggccttggtc agccggtcga gcaggtagta ctcgcccttg
     1921 cgcggcttga tctcgaactc ctcggcgccg aacgcgcgag acacctcgtc ggcatagagg
     1981 cccgcggcgt tgaccacgta gcgcgccttg acgacgcggc catccgcagc gcgtaccgtc
     2041 cactcgtcgc cggcgcgcgc cgcgtgctcg accttgaagc cggtcatg
//
LOCUS       DAOOLN010000262         2085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_67139_length_2085_cov_3.305911, whole genome shotgun sequence.
ACCESSION   DAOOLN010000262 DAOOLN010000000
VERSION     DAOOLN010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67139_length_2085_cov_3.305911"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1739)
                     /locus_tag="PLW80_04195"
     CDS             complement(<1..1739)
                     /locus_tag="PLW80_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65737.1"
                     /translation="MIKKRANTAAFVRIAALAALCLPWSRARAQALYSVLPEEPPSSL
                     FSTKLGDSEVEAFAQGFWEASILSAGAWSIDSGGSSFGANPPFQFMQTPDLYVFLSLR
                     RTWFFEAYVTNEAADNRFTLAFEGDDGDFIKFARLGNSGISMPSYPFMSFGSPDGSFG
                     AAMSASDVASGVSLDAMVRWDGLRWETRDFFGSSEMIETELAPGDDLRGRRFVLPSGG
                     LVSVELYEARTSGPRRLASDEYSASLASGVILLAAEPSGELTAAYVDSLGARASVTLY
                     AFAEDDDGDPVRRYNRYEARNLYALPDTASARQLFVRDLATGTVDTRFEVGRVGPGLV
                     QVLRSGSGPDPDDDGYMRPFVDLTPWSYDRSNGGGDATVASEGFAIVARSSETVEAIM
                     LGDGAVAGTISVYRDGVASSSFGYDEAGRTLTLSPPPRAGESIRVRYAVSTTDGADGA
                     LAFGVGARFPWLGQSWSTAIGGRWPLFGLGYETGDSMQPAWLGISAGVTGRAGVVDGI
                     AATGAAVPVAYTLEAMARYLRAGSRGLYRVVGMEDYGDSHWLEPFRQVSPEDGGITAS
                     VVVDAELAASDAFG"
     gene            1764..>2085
                     /locus_tag="PLW80_04200"
     CDS             1764..>2085
                     /locus_tag="PLW80_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65738.1"
                     /translation="MERVIEKTRRSGAVSVIASSTMDALAIVSIVFAFVAAGMAAAGA
                     SWRASAFMAIVGVLFDILFSYEFFLGAIGRPSPFPWLSGLSSALPLLLISGPFLAGWV
                     GADLG"
BASE COUNT          329 a          736 c          684 g          336 t
ORIGIN      
        1 ccgaaggcgt cggacgcggc cagctcggcg tcgacgacga cggaggccgt tatgccgccg
       61 tcctccggcg atacctgcct gaacggctcg agccagtgcg agtcgccgta atcctccatg
      121 ccgaccaccc ggtacagccc ccgggaaccg gcgcgcaggt agcgtgccat cgcctcgagg
      181 gtatacgcca ccggaacggc cgcgcccgtt gcggcgattc cgtccacgac gccggctcgg
      241 ccggttacgc cggccgatat gcctagccag gccggctgca tggagtcgcc ggtctcgtaa
      301 ccgagtccga agagtggcca acggccgcct atggcggtcg accagctctg gccgagccag
      361 ggaaagcgcg ccccgacgcc gaaggcgagc gctccgtctg ccccgtccgt ggtcgacacg
      421 gcgtaccgca cccgtatgct ctcgccggcc ctcggcggcg gcgatagcgt gagcgttcgg
      481 cccgcctcgt catagccgaa cgacgacgaa gcgaccccat cacggtagac cgagatcgtc
      541 ccggccacgg cgccgtcgcc gagcatgatt gcctcgaccg tctctgatga cctggcgacg
      601 atggcgaagc cctcgctggc caccgtcgcg tcgccgccgc cgttcgacct atcgtagctc
      661 cacggagtca ggtcgacgaa cggccgcatg tatccgtcgt cgtcgggatc cggtcccgat
      721 cccgagcgca ggacctgtac caggcccggg ccgacgcggc cgacctcgaa gcgggtatcg
      781 acggtaccgg tggccaggtc gcggacgaac agctgcctgg ccgaggccgt gtctggtagc
      841 gcgtacagat tgcgcgcctc gtagcggttg tatcgtcgta ccgggtcgcc gtcgtcatcc
      901 tccgcgaagg cgtagagcgt gacgctcgcc cgagcgccga gcgaatcgac gtaggctgcc
      961 gtcagctcgc ccgacggctc cgcggcgagc aggatgacgc ccgaggccag ggaggccgag
     1021 tactcgtcgc tagccaatcg cctcgggccg ctcgtgcgcg cctcgtacag ctcgaccgag
     1081 acgaggccgc cggaaggcag cacgaaacgc cggccgcgca ggtcgtcgcc aggggccagc
     1141 tccgtctcga tcatctccga cgagccgaag aagtcccggg tctcccagcg gaggccgtcc
     1201 cagcggacca tcgcgtcgag cgacacgccg ctcgccacgt cgctggcgct catcgccgcg
     1261 ccgaaagagc cgtccggaga gccgaacgac atgaacggat aggacggcat cgatatgccc
     1321 gagttgccga gccgggcgaa cttgatgaaa tccccatcgt cgccctcgaa ggccagcgtg
     1381 aaccgattat ccgcggcctc gttcgtcaca taggcctcga agaaccatgt tcgcctgagg
     1441 ctcaggaata cgtataggtc gggcgtctgc atgaactgga acggcggatt ggcaccgaag
     1501 ctcgatccgc cggagtctat cgaccaggcg ccggcgctca ggatcgacgc ctcccagaag
     1561 ccctgggcga aggcttccac ctccgagtcc ccgagcttgg tcgagaacag gctcgatggc
     1621 ggctcctcag gcaggaccga gtacagggct tgcgcccgcg cccgggacca ggggaggcac
     1681 agcgccgcca gcgcggctat gcggacgaag gccgccgtat tcgctctttt ctttatcatc
     1741 gttcgcgtat atcattgtag cccatggaac gcgttattga gaagacgcgc cgctccggcg
     1801 ccgtatcggt catcgcctcg tcgaccatgg acgctttggc gatcgtctcg atcgtcttcg
     1861 cgttcgtcgc cgcaggcatg gcggcggccg gcgcgtcgtg gcgcgcctcg gccttcatgg
     1921 ccatcgtcgg cgtgctgttc gatatcctat tctcgtacga gttcttcctc ggggccatcg
     1981 gacgtccttc gcccttccca tggctgtccg ggctgtcgtc ggccctgccc ctgctcctga
     2041 tctccggccc gttcctggcg ggctgggtgg gagccgacct gggct
//
LOCUS       DAOOLN010000263         2085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_67140_length_2085_cov_3.288670, whole genome shotgun sequence.
ACCESSION   DAOOLN010000263 DAOOLN010000000
VERSION     DAOOLN010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67140_length_2085_cov_3.288670"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PLW80_04205"
     CDS             complement(<1..531)
                     /locus_tag="PLW80_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB65739.1"
                     /translation="MHSDTTSITAIAQAPKRRDDRREELFAAALRQYAKHGYKRTRVE
                     DIAAELGLTKGAVYFYAKGKEELYREAISWALGEWCSAIERSAARASSSVERVSILSR
                     SSFAYVEAHHDLRSVIARDPSIFSLDPASDRFASVNDRARGLLESAIRSGVASGEFRS
                     TIDPSAASRFLYQVYIT"
     assembly_gap    767..866
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1069..>2085)
                     /locus_tag="PLW80_04210"
     CDS             complement(1069..>2085)
                     /locus_tag="PLW80_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB65740.1"
                     /translation="DRYFLDNVAGWILELDRGEGIPWKGNYSSWLDQKSKQLELEEKG
                     NSERAKTLERELEWVRMSPKGRHAKSKARIAQYEKLLADGEREKVRDTKLVITPGPRL
                     GQVVIEAKGLAKSFGDKCLFQGLEFTVPPGAVVGIIGPNGAGKTTLFKMIAGLEQPDG
                     GSLKLGDTVKLAYADQMRAGLDPDKTVWQQLSGGLDIIKLGGVKEVNSRAYCSWFNFS
                     GGDQQKKVGVLSGGERNRLNLAMMLKEGANVILLDEPTNDLDVNTLRALEEALDGFAG
                     SALVVSHDRWFLDRVCTHLLAFEDGEVVWYDGNWSEYAEYRREKLGGDADRPHRYVYR
                     KLER"
BASE COUNT          312 a          628 c          652 g          393 t
ORIGIN      
        1 ggttatgtag acctggtaca ggaagcgcga ggcggcggag gggtctatcg tcgacctgaa
       61 ttcgcccgat gccactcccg agcgaatagc gctctcgagc aggccgcgcg cgcggtcgtt
      121 gaccgaggcg aagcggtccg acgccgggtc gaggctaaag atggaaggat cgcgcgctat
      181 gacggagcgg aggtcgtgat gagcctcgac gtaggcgaac gacgatcgtg atagtatcga
      241 tacgcgctcc acggaggagg aggcgcgagc ggccgaccgc tctatggccg agcaccactc
      301 gccgagcgcc catgatatgg cctcgcgata gagctcttcc ttgcccttcg cgtagaaata
      361 gaccgcgccc ttggtcagac cgagctcggc ggctatgtcc tcgacgcggg ttcgcttgta
      421 gccatgcttg gcgtactgcc gaagggccgc ggcgaagagt tcctcgcgcc tatcgtcgcg
      481 ccgcttcgga gcctgggcga tggcggtaat actagtagta tccgagtgca ttattaaata
      541 attgaacgga aagtttaatt ggtcaatgtg tttctatgta aacaacccgg cccaggcact
      601 ccataccgtt cggtttcgaa cggaggctca gcccgcgcga agccgggctt gccgaaacgc
      661 cgaccgcccg cgggccggat ggtcggcggc cgactatggt atctttccag cttcctgtac
      721 acgtagcggt gcgggctcgt ctggcctccg gccagccgta ccggttnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnncgga accggctgat ccgtgacgcc gaccgcccgc
      901 gggccggatg gtcggcggcc gactatggta tctttccagt ttcctgtaca cgtagcggtg
      961 cgggctcgtc tggcctccgg ccagccgtac cggttccgcc aagggagcgg aaccggctga
     1021 tccgtgacgc cgaccgcccg cgggccggat ggtcggcggc cgcttttcct atctttccag
     1081 cttcctgtag acgtagcggt gcgggcggtc ggcgtctccg ccgagcttct cgcggcggta
     1141 ctcggcgtac tctgaccagt tgccgtcgta ccagaccacc tcgccgtcct cgaaggccag
     1201 gagatgggtg cagacgcgat cgaggaacca gcggtcgtgg ctgacgacga gggccgagcc
     1261 cgcgaaaccg tcgagggcct cctcgagggc gcgtagcgtg ttgacgtcca ggtcgttcgt
     1321 cggctcgtcg aggaggatca cgttggcgcc ctccttgagc atcatcgcca ggttaagtcg
     1381 gttgcgctct ccgccggata gtacgccgac cttcttctgc tggtcgccgc cggagaagtt
     1441 gaaccaagag cagtaggccc tggagttcac ttccttgacg ccgccgagct tgatgatgtc
     1501 gaggcctccg gacagctgtt gccagacggt cttatcgggg tcgaggccgg cgcgcatctg
     1561 gtcggcatag gccaacttga ccgtatcgcc gagtttgagg gagcctccgt cgggctgttc
     1621 caggccggct atcatcttga acagagtcgt cttgccggcg ccgttcggcc ctatgatgcc
     1681 gacgacggct cccggcggga cggtgaactc gaggccctgg aacaggcact tgtcgccgaa
     1741 cgacttggcc aggcccttgg cctcgatgac gacctgcccg agcctcgggc cgggcgtgat
     1801 gacgagcttc gtatctctga ccttttcgcg ctctccgtcg gccaggagtt tctcgtactg
     1861 ggcgatgcgc gccttgctct tcgcgtggcg gcccttgggg ctcatgcgca cccactcgag
     1921 ctccctctcc agggtcttgg cgcgctcgga gttgcccttc tcctcgagct cgagctgctt
     1981 cgacttctgg tcgagccagc tcgagtagtt gcccttccac gggatgccct cgccgcggtc
     2041 cagctccagg atccagccgg ccacgttgtc caggaagtag cggtc
//
LOCUS       DAOOLN010000264         2078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_67470_length_2078_cov_3.507662, whole genome shotgun sequence.
ACCESSION   DAOOLN010000264 DAOOLN010000000
VERSION     DAOOLN010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67470_length_2078_cov_3.507662"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PLW80_04215"
     CDS             complement(<1..638)
                     /locus_tag="PLW80_04215"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HPB65741.1"
                     /translation="MIIVLAGPSGSGKTSSCGIAVRLAREAGVRVAGMLCEAVFSGGS
                     KVGIDWRDLADEGAARRSLARRLPGFSQARSAGAPSQGRSPAFDDSDPHTVRFGSWEF
                     DRKALAEADAATAAAVSGGSGPKGAAPSLVIVDEIGPLELERGSGMVKTLMALDEAAS
                     PEWERAGDAGRLGLTVVTARPDIADRLAARWPGSVRLDVSDVPFERTARRIL"
     gene            complement(647..1327)
                     /locus_tag="PLW80_04220"
     CDS             complement(647..1327)
                     /locus_tag="PLW80_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65742.1"
                     /translation="MEAKLAPWRSGRVGFIAGLSVAAVLVGAGVYLGVAVSSAPLAPA
                     VESSFSSYVEGLSATGKIVVVEARERLEIRRTTPGLLFGDTTVGRLLGIRSDATVEAS
                     AWAELSFIIDLKATESWSVRYDPADGGRLSVAAPPIAMLTPAIHTDTITILTTDRSVF
                     LDERLLEARVMRDLTARFVEAASAMLDEPELRSKAAAAIEGIARSFSRTAGFPLASVD
                     VSFAPAED"
     gene            complement(1377..1907)
                     /locus_tag="PLW80_04225"
     CDS             complement(1377..1907)
                     /locus_tag="PLW80_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPB65743.1"
                     /translation="MRILVVSDTHGDFRGLVAVLGLLGRSVQALIHLGDGSGDVKAAM
                     RYGAPTPPAFVVRGNVDADSSVPLARRFDAEGRLLILAHGHRYPLGDGTSSLVYAAKE
                     AGARAFFFGHTHVPYCEERSGVLLLNPGSLSRPRGPWGPSLAIVEAPPSSSCLDVKLF
                     ELVGPQAAPRLRAIRL"
BASE COUNT          315 a          729 c          724 g          310 t
ORIGIN      
        1 tctaggatac gcctcgctgt ccgttcgaac gggacgtccg agacgtcgag cctgacggag
       61 cctggccagc gagcggctag cctgtcggcg atgtcgggcc tggcggtgac gaccgtgagc
      121 ccgaggcgcc ccgcgtcgcc cgctcgctcc cattctgggc tggccgcctc gtcgagggcc
      181 atgagcgtct ttaccatgcc ggagccacgt tccagctcga gcggccctat ctcgtcgacg
      241 atgaccaggc tgggcgccgc gcctttcggc ccggatcccc cggagacggc cgccgccgtc
      301 gcagcgtcgg cctcggcgag ggcttttcta tcgaattccc aggagccgaa tcgcacggtg
      361 tgcgggtccg agtcgtcgaa ggcggggctc cggccttggg acggagcgcc cgccgaccga
      421 gcctgcgaga agcccggcag gcgcctggcc agcgagcgcc gcgccgcccc ctcgtccgcc
      481 aggtcgcgcc agtcgatgcc gaccttcgag ccgcccgaga agacggcctc gcagagcatg
      541 cccgctaccc ggaccccggc ttctctggcc aatcgtacgg cgatgccgca ggagctggtc
      601 ttgcccgagc cgctcggccc cgcgagcacg atgatcatgg ctcgcctcag tcctcggccg
      661 gtgcgaacga gacatctacc gacgccagcg gaaagccggc ggtccgcgag aacgacctgg
      721 ctattccctc gatcgcggcg gccgctttgg accgcagctc cggctcgtcg agcatcgccg
      781 aggccgcctc gacgaagcgc gccgtcaggt cgcgcatgac gcgggcctcg agcagccgct
      841 cgtcgaggaa tacggaacgg tcggtggtga ggatcgtgat cgtatcggta tgtatggccg
      901 gcgtcagcat cgcgatcggc ggcgcggcga cgctgagcct gccgccatcg gccgggtcgt
      961 agcgtaccga ccaggattcc gtggctttca ggtcgatgat gaacgacagc tccgcccagg
     1021 ccgaggcctc gacggtggcg tcggagcgga tgccgagcag tcggccgacc gtcgtatcgc
     1081 cgaacaggag ccccggcgtc gtcctgcgga tctcgagccg ctcgcgggcc tcgacgacga
     1141 ctatcttgcc ggtggccgat agaccctcga cgtaggagct gaacgagctc tccacggccg
     1201 gggcaagtgg ggcgctcgat acggctacgc ctaggtatac gccggctccc acgaggacgg
     1261 cggccaccga gagcccggct atgaacccga ctcgtccgga tcgccacggg gctagcttcg
     1321 cttccataac cggtactatc cagcttttct gccctgggcg caagtccggc tccgggctat
     1381 agccgtatcg cccggagcct cggggccgcc tgggggccga ccaactcgaa cagcttgacg
     1441 tcgaggcagc tcgacgacgg gggcgcctcg acgatggcca ggctcggtcc ccacggcccg
     1501 cgcgggcggg ataggctgcc gggattgagg aggagtaccc cggatcgttc ctcgcagtac
     1561 gggacgtggg tgtggccgaa gaagaaggcc cgcgcgcctg cctccttggc cgcgtagacg
     1621 aggctggagg tgccgtcgcc aagggggtag cggtggccgt gcgccaggat aaggagccgt
     1681 ccctcggcgt cgaagcggcg agcgaggggc accgacgagt cggcgtcgac gttgccccgt
     1741 acgacgaagg ccggcggcgt cggggctccg tagcgcatcg cggccttcac gtctcccgac
     1801 ccgtccccga ggtggatcag ggcctggacc gaacgtccga gcaggccgag caccgcgacg
     1861 agtccccgga aatccccgtg ggtatccgag acgaccagaa tcctcatcgc tcgccggacc
     1921 ttccgacgcg ctcgttgttc gccttgagcc tggccgccat cagctcgacc agccggcgcg
     1981 acgcgccggc gtcctgggcc aggtatcgct cgaacatctc gggtggcaac acgaatacca
     2041 tgcagtcggt cttggcgacc gctcgcgctg acctgggc
//
LOCUS       DAOOLN010000265         2077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_67499_length_2077_cov_4.013848, whole genome shotgun sequence.
ACCESSION   DAOOLN010000265 DAOOLN010000000
VERSION     DAOOLN010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67499_length_2077_cov_4.013848"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..786
                     /locus_tag="PLW80_04230"
     CDS             277..786
                     /locus_tag="PLW80_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65744.1"
                     /translation="MKLFRAIATIMVMSLVAIPAVAQGSSEAGKADPRVAQALDSLGL
                     KYSVNSSNNYKVVYSMEDGSGRSHLAFVVSKTSKYRSLEIREIWSIAAILDEYPDAEV
                     VDYMFTMNSTTKIGAWAIEVSDDGEVWVMYTVKVPADLSADELNDIIYFVAEVCDELE
                     VELTGDDEY"
     gene            complement(1090..1448)
                     /gene="ssrA"
                     /locus_tag="PLW80_04235"
     tmRNA           complement(1090..1448)
                     /gene="ssrA"
                     /locus_tag="PLW80_04235"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1524..>2077)
                     /locus_tag="PLW80_04240"
     CDS             complement(1524..>2077)
                     /locus_tag="PLW80_04240"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPB65745.1"
                     /translation="RVAMTNSRYYTEARRLGLEVADKPESQDFIPGGYGALFDEQEPG
                     DIVDERGKVLGRHRGIVNYTIGQRRGLGVSTGPEPLYVAALDAERNRVVVTGDDRLFC
                     SGIEGGDAVLHAEELAGRRFEAFVRIRQNHRPAKAFVTVDGQAARVEFDSAQRAAAPG
                     QSAVFYDADGFVLGGCVIERALR"
BASE COUNT          337 a          680 c          682 g          378 t
ORIGIN      
        1 ccgaggtacc cgacctcatg gtcgcgctgc ggttgtcggt gttcgacttc gtgcgcatac
       61 gcagttggct ggcagtcgac gacgccgacg agcttctcat cgccgacacg gcgctactgg
      121 ccggggccga gggcctgttc gctcatgcga gatcggccct acggcgctcc gctccgtgag
      181 tggtacgaag attgtggacc ctgtaattca agcgccgacg gactatacta gcgatacgcg
      241 cttatatcga ttctaccgat aggaagggag caatctatga aactgttcag ggctatcgcg
      301 acgatcatgg tcatgtcgct cgtcgccatt cccgccgtcg cccaaggtag ctcggaggcg
      361 ggcaaggccg atcctcgggt ggcccaggcg ctcgactcct tgggcctcaa gtactcggtc
      421 aactcgtcga ataactacaa agtggtctac agcatggaag acggctccgg ccgctcgcac
      481 ctggccttcg tcgtctccaa gacctccaag taccggagcc ttgaaatacg ggagatatgg
      541 tcgatcgcgg ccatcctgga cgagtatccg gacgccgagg tcgtcgatta catgttcacg
      601 atgaactcga cgaccaagat cggcgcttgg gccatcgagg tgtcggacga cggcgaggtc
      661 tgggtcatgt atacggtcaa ggtgcccgcc gacctgtcag cggacgagct caacgatatc
      721 atctacttcg tcgccgaggt ctgcgacgag ctcgaggtcg agttgactgg cgacgacgag
      781 tactgatccc gtaccttctc acgggttacc cgctcgcgta caacgagaag ccccgccttg
      841 cggcggggct tctcgtgttt ggaggtggcg agagtcggct tccagtcgcg cgcgtcctgc
      901 gcgctccttc cagcccgcct acgggccggt gctcgctcct gctcgcacat cggcaggggc
      961 gcaaggggga taccccctcg agcgacggcg agccgccgct ctcaccccag cgccgctgcg
     1021 agtctcgcgt cgcatggttc gactcccccg aaaacaagtc aggctccgct ttcgcggagc
     1081 ctgacttgtt tttggaggtg gggggagtcg aacccccgtc ctatcacgcg gaaggaaggc
     1141 ttctacaggt ttaggcggcc tttcaagttg tcgggaggcg ggcggcgggc cgccgcgcta
     1201 tcgcctccgt atcccgcttg gtgtcccccc cgtcccgcgg gcgtggcggg gggtaagccc
     1261 tggtcgggtt acagaccggt cgcggcgctc agagcggggc gccgcggagt ccgtcgctat
     1321 cggcgcttac gcggcgagag cgaaattgcc gttgtcgttg gcaattaaag gtttgccgag
     1381 agtaggagat cggcgctcca cctgcgaccc gcgaaccgga ccgtgacagt cgaaaccggg
     1441 gcacccccgt acatggctac aatactgatt gtcgccgcat gggtcaagtt gacgaggggg
     1501 acagccgtcg cgccgccacg gccctaacgc agcgcccgct ctatgacgca tccgccgagc
     1561 acgaagccgt cggcgtcgta gaagacggcc gactggccag gcgccgcggc gcgctgcgcc
     1621 gagtcgaact cgacgcgggc cgcctggccg tcgaccgtga cgaacgcctt ggccggccta
     1681 tggttctgcc tgatgcgcac gaaggcctcg aagcgccggc cggccagctc ctctgcgtgg
     1741 aggaccgcgt cgccgccctc gatgccggag cagaagagcc tatcgtcgcc cgtgaccacg
     1801 acccggttgc gctccgcgtc cagggccgcc acgtagaggg gctcgggccc cgtcgagaca
     1861 ccgagtcccc tgcgctggcc tatcgtgtag ttgacgatgc cccggtgcct gccgagtacc
     1921 ttgccccgct cgtcgacgat gtcgcccggc tcttgctcgt cgaacagcgc gccgtagccg
     1981 ccgggtatga agtcctggct ctccggcttg tcggcgacct cgaggccgag cctgcgggcc
     2041 tcggtgtagt aacggctgtt cgtcatggcg acgcggg
//
LOCUS       DAOOLN010000266         2077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_67532_length_2077_cov_2.050445, whole genome shotgun sequence.
ACCESSION   DAOOLN010000266 DAOOLN010000000
VERSION     DAOOLN010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67532_length_2077_cov_2.050445"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..864
                     /locus_tag="PLW80_04245"
     CDS             103..864
                     /locus_tag="PLW80_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB65746.1"
                     /translation="MRNPGTLIVGRALRRLVSNPVPLLSGLGMSAFFLFVYDAAIGGI
                     GFLPEFGGAGYMAFLLPMGVISLLFGSSAGSAQALSRDIGSGYFMRLALAPVPRSAFV
                     LAAILADAAGIFVSSLIVLGLGALLGAPLLGGAVGVFGTAALATLFGAGISAASAAAV
                     MRTGKVELAGTIGSAVFMLLFLAPTFVPRELMGARWLQVVSAWNPLSYLMEAMRVMVS
                     GLGEPSSVPIAFAVAAVAGLGGTAAACLSVRKVLN"
     gene            complement(918..1679)
                     /locus_tag="PLW80_04250"
     CDS             complement(918..1679)
                     /locus_tag="PLW80_04250"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPB65747.1"
                     /translation="MATRGSFRTVDVARAAGTHENTVRLYVAWGFLAEPERAANGYRR
                     WTAEHIDQMLFARLAMHGMWPGRPIRESALALVRRAATGDLTGAVADARIHAALVDAE
                     LARAESAAAFLERWAAGAAPAAGDEGRLGPREAAAAVGATPGQIRNWERNRLVASVKD
                     PRTGHRVYGPDQIGRLRVVRSLLLAGYSVMAILRMATELDRGRSTGLKDVLNTPRPGE
                     EALTAFDAWLDSLAEQKARAERLIAMLAARIEANR"
     gene            complement(1690..>2077)
                     /locus_tag="PLW80_04255"
     CDS             complement(1690..>2077)
                     /locus_tag="PLW80_04255"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HPB65748.1"
                     /translation="YDWVTGFGLSGKSRRVGFNLTDNQVREQDRYNENVLWVNSRVYP
                     LPPIKVTRPNGVDNPWHVQDTEGLVDLVFVPERKNDLRINLLFASVDYHGPFGTFSGT
                     LRSPDGSVKVDAEGLLGMGEQKYLRA"
BASE COUNT          266 a          690 c          741 g          380 t
ORIGIN      
        1 tcgcggcggc gcacgccggc cccggcgggg acctcgaggc gggctacctg aattttatcg
       61 agtcgcggcg ccgaggcgcg ttatcggaga aggaggccgt cggtgcgtaa tcccggaacg
      121 cttatagtcg gacgggcgct caggcggctc gtttccaatc cggtgccgct actgtccggc
      181 ctgggcatga gtgcgttctt cctattcgtc tacgacgcgg cgataggcgg catcggcttc
      241 ctacctgaat tcggcggtgc ggggtatatg gccttcctgc tacccatggg cgtcatatcg
      301 ctcttgttcg ggtcgtcggc gggctcggcc caggctctga gccgggatat cggctcaggc
      361 tattttatgc ggctggcgct ggcgcccgta ccgaggtcgg ccttcgtgct cgcggcaatc
      421 ctggccgatg cggcgggcat cttcgtgtcg tcgcttatcg tgctgggcct gggcgcgctg
      481 ctcggggcgc cgctcctggg aggggccgtc ggcgtgttcg gaaccgcggc tctagccacg
      541 ctgttcggcg ccgggatatc cgcggcctcg gctgccgcgg tgatgcggac gggcaaggtg
      601 gagctggcgg ggacgatagg ctcggccgtc ttcatgctgc tgttcctcgc cccgaccttc
      661 gtgccgcggg agctgatggg cgctcgctgg ctccaggtcg tctcggcgtg gaacccgctc
      721 agctacctga tggaggcgat gcgggtcatg gtatcgggcc tgggagagcc gagttcggta
      781 cctatcgcgt tcgccgtcgc ggcggtggcc gggctcggcg gcacggcggc cgcgtgcctc
      841 tccgtcagga aggtgctgaa ctagggctgg aggcgacgag tcggaggatg ggcgccgtgg
      901 ttcaggccgc gaacgcgcta acggttggct tcaatcctgg ccgcgagcat cgctatgagg
      961 cgctccgccc gcgccttctg ctcggccagc gagtcgagcc aagcgtcgaa ggcggtcagc
     1021 gcttcctcgc ctgggcgcgg cgtgttgagg acgtccttga gtccggtcga gcggccccgg
     1081 tcgagttccg tcgccatgcg cagtatcgcc atgacgctgt agccggcgag cagaagggat
     1141 cgaaccaccc gcagccggcc tatctggtcc ggcccgtaga cccgatgtcc ggtgcgcggg
     1201 tccttgaccg acgctacgag gcggttgcgc tcccagttcc ggatctggcc cggcgtcgcg
     1261 cctacggccg ccgcggcctc tcgggggccg agccgcccct cgtcgcccgc ggcgggggcc
     1321 gctcccgccg cccagcgctc caggaaggcg gccgcgctct cggcgcgcgc tagctcggcg
     1381 tccacgaggg ccgcgtggat gcgggcgtcg gctacggccc cggtcaggtc tcccgtcgcg
     1441 gcgcgacgaa cgagcgccag cgcggattct cgtatgggcc ttccgggcca catgccgtgc
     1501 atcgctagcc gtgcgaatag catctggtcg atatgctcgg ccgtccagcg cctgtagccg
     1561 ttggcggcgc gctctggctc cgcgagaaaa ccccaggcga cgtagagcct gaccgtattc
     1621 tcgtgcgttc ccgccgccct ggcgacgtcc accgtacgga acgagcctct cgtcgccatg
     1681 cgccttccgt tacgcccgaa ggtacttctg ctcgcccatg ccgaggaggc cctcggcgtc
     1741 gaccttgacc gagccgtcgg gcgagcgcag cgtgcccgag aaggtaccga acggcccgtg
     1801 gtagtcgacc gaggcgaaga gcaggttgat ccgcaggtcg ttcttgcgct cgggtacgaa
     1861 gaccaggtcg acgaggccct cggtatcctg cacgtgccag gggttgtcga cgccgttcgg
     1921 cctggtcacc ttgatcggcg gcagggggta gactcggctg ttcacccaga gcacgttctc
     1981 gttatagcga tcctgctcgc ggacttggtt gtcggtcagg ttaaaaccga cccggcggct
     2041 cttgcccgac agcccgaacc cggtaaccca gtcatag
//
LOCUS       DAOOLN010000267         2075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_67629_length_2075_cov_2.728218, whole genome shotgun sequence.
ACCESSION   DAOOLN010000267 DAOOLN010000000
VERSION     DAOOLN010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67629_length_2075_cov_2.728218"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="PLW80_04260"
     CDS             complement(<1..802)
                     /locus_tag="PLW80_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HPB65749.1"
                     /translation="MRRRTILVLILVTAFACLYGESRSTSVDFVLLVDTSLSMADAID
                     DAKRYAAGEVIGRLVEPGDWVSVLEFYGRSETVWQGDVRGEEDVAAIVRSLNSLVADG
                     RFTDIGGVLDEMDTLILSRGQPDRPKYILLITDERQEAPEGTRYYAPDYVVRHPLLEY
                     VKRVDLGAFRVITIGYGLSSRIEGEARSLMTTLSEPPARREPTLPGAPSGVAAADGTA
                     SIGTEPPETVGPGAETGTSSGTSFGTGAATEATSRAADGSGKASFAGAP"
     gene            1001..>2075
                     /gene="cfpA"
                     /locus_tag="PLW80_04265"
     CDS             1001..>2075
                     /gene="cfpA"
                     /locus_tag="PLW80_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014342563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic filament protein CfpA"
                     /protein_id="HPB65750.1"
                     /translation="MGAIDLPKSPNVYHPEKPSAVGSRNSLAQENRDQQKEVDQLLHE
                     ETNKVLHHINSRLPKEVLERLDVMGGLKEKVYNYFNQNYQNMFNRYIVTTEDEMVKKV
                     RNFIDKEEMKSLARYTPKEIATLLDEVGGADKFNTGEIEKSMVNMFGHLQGHIQRGVN
                     ELENLTNSLLRQKTDVGAFIRGENAYSVVKCAFKDNTYKPKTVTDVKLSVNILDSELI
                     SPIFHYQVTVEYLIKDLIAKHIIDTIDKDIEALKDQLVDEGKEELNDTEIIFERMKKV
                     KDHTDDEVENVKSKRYSIVAKDLMDRINNLRAEIDPSTFDQLNIRENLKKIIDIENIR
                     NRGFNTAVNSITSILDTSKMGYQY"
BASE COUNT          498 a          613 c          585 g          379 t
ORIGIN      
        1 agggcgcgcc ggcgaaggag gccttgccgg aaccgtcggc agcgcgcgac gtcgcctccg
       61 tagcggcgcc cgttccgaac gaggttccgg acgaggttcc ggtctccgct ccgggaccca
      121 ctgtctccgg cggctcagtc ccgatactcg ccgttccgtc ggccgccgcg acgcccgaag
      181 gcgcaccggg taaagtcggc tcccgcctgg ccggcggttc ggacagggtg gtcatcagcg
      241 agcgcgcctc gccttcgatg cgcgacgaga ggccgtagcc tatggtgatg acgcggaacg
      301 cccccaggtc gacgcgcttg acgtactcga gtagcggatg cctgactacg tagtcgggag
      361 cgtaatagcg ggtgccctcc ggcgcctcct ggcgctcgtc ggtgataagc aggatgtact
      421 tagggcgatc cggctgacct cgggacagga tcagggtatc catctcgtcg aggacgccgc
      481 cgatgtcggt aaagcggccg tcggcgacca gagagttcag gctacgcacg atggcggcga
      541 cgtcttcctc gccccgtacg tctccctgcc agacagtctc ggatcggccg tagaactcca
      601 ggacgcttac ccagtcgccc ggctcgacga ggcgcccgat gacctccccg gctgcgtagc
      661 gcttggcgtc gtcgatagcg tcggccatag acagggatgt gtcgacgagc agtacgaaat
      721 cgacgctcgt cgatcgcgac tcgccgtaca ggcaggcgaa cgccgttacg agtataagta
      781 ctagtatggt tcgtcttctc atgctgcctt ctatcgttat tttattgaca gaaacagcat
      841 tgcctatttt ttacctattt gcaagctaaa aaggcgtatg aacgatatct tccattaaaa
      901 ttcaaatcat atccagtgga aaaaaagccg aagagcttta cattttagcg tttcggggta
      961 gtattgacgg gtatcgaaat tagaccgcaa ggagaaccac atgggcgcca tagatcttcc
     1021 caagagtcct aacgtttatc atcccgagaa gccgagcgcc gtcggctccc gaaactctct
     1081 ggcacaggag aaccgagacc agcagaaaga ggtcgatcag ctcctgcacg aggagacgaa
     1141 taaggttctc caccacatca actccaggct ccccaaggag gtgctcgagc gcctcgacgt
     1201 gatgggcggc ctcaaggaga aggtgtacaa ctacttcaac cagaactacc agaacatgtt
     1261 caatcgctat atcgtcacga ccgaggacga gatggtgaag aaggtcagga acttcatcga
     1321 caaggaagag atgaagtccc tggcgcgcta taccccaaag gaaatagcga ccctgctcga
     1381 cgaggtcggc ggcgccgata agtttaacac cggcgagatc gagaagtcga tggtcaatat
     1441 gtttggccat ctccaggggc atattcagcg cggcgtcaac gagctggaga acctgaccaa
     1501 ctcgctcctc aggcagaaga ccgatgtcgg tgcgttcatc cgcggcgaga acgcctactc
     1561 ggtggtcaag tgcgccttta aggataacac ctacaagccc aagaccgtca ccgacgtcaa
     1621 gctgtcggtc aacatcctcg actccgagct gatcagcccg atcttccatt atcaggtcac
     1681 cgtcgagtac ctgatcaagg acttgatcgc caagcacatc atagacacga tcgacaagga
     1741 catcgaggcg ctcaaggacc agctggtcga cgagggcaag gaagaactga acgataccga
     1801 gatcatcttc gagcgcatga agaaggttaa ggatcacacc gacgacgagg tcgagaacgt
     1861 caagtcgaag cgctactcga tagtggccaa ggacctgatg gacaggataa ataatctccg
     1921 cgccgagatc gacccgtcca ccttcgacca gctcaacatc cgcgagaacc tcaagaagat
     1981 catcgatatc gagaacatcc gcaaccgcgg cttcaacacc gccgtcaact cgatcacctc
     2041 gattctcgac acctcgaaga tgggctacca gtaca
//
LOCUS       DAOOLN010000268         2065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_68129_length_2065_cov_2.479104, whole genome shotgun sequence.
ACCESSION   DAOOLN010000268 DAOOLN010000000
VERSION     DAOOLN010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68129_length_2065_cov_2.479104"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PLW80_04270"
     CDS             complement(<1..588)
                     /locus_tag="PLW80_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65751.1"
                     /translation="MAQRKSGTRGGPSKKKGAGCLVWLVMMLVTLLLFVVNWDVIKET
                     LESTGFIEAVGKPGDDRAAGDEPSRGDEPSRGDGSLEPTAGREGPIVVAPEPAVGETA
                     QPKPAGRPETAPEAARPSTDEPVAKPITSPMEQIKATRVAVLYFIRVDDDGTLSRQEA
                     KRTVGASDSPMTDALKALMAGPSADELGRGLISLIP"
     gene            complement(623..1735)
                     /locus_tag="PLW80_04275"
     CDS             complement(623..1735)
                     /locus_tag="PLW80_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB65752.1"
                     /translation="MNTDESPRLPGNIAFIRIPEGMARPIGSFTVDPSIPIPVELGQG
                     APDLSDLSWEMIVAGMLRLLAYDPDNPDGAYYRAFVTAVKPELFAELSEAGILKARNG
                     DLEVAEEIFKALAGLSPEAPEPTLNLAILYEDKADALDRSGKEELADAMRSRAFEAYK
                     RLLAMEPAYPDAFFNAGLFYLKNREYGQAARLFESYAAMGDDEAKLSKAREVLSKLRV
                     RGDADAKFKEAYDFIRMGREEEGIERAMEFNRGNDGVWNGWFLVGWGHRRLGRYEDGR
                     AAFLRALELGADEVDLLNELAICEMELGMLSESRGRLERALRKEPENIKIISNLGVVA
                     RRQGRDEEAAGFFRIVLEMEPGDALARAQLDELTSA"
     gene            complement(1783..>2065)
                     /locus_tag="PLW80_04280"
     CDS             complement(1783..>2065)
                     /locus_tag="PLW80_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HPB65753.1"
                     /translation="SFSGAKVIIVGLAKFLVGKSCRLRVDLEDPRETVVVSGSVVRYE
                     DVEGRKDLTAIAVRFDEATVPMSYKMHLNDYLGQKKNQADDDQGGAPAP"
BASE COUNT          305 a          696 c          676 g          388 t
ORIGIN      
        1 cggtatcagg ctgatcagcc cgcggccgag ctcgtcggcg ctcgggccgg ccataagggc
       61 cttgagtgcg tcggtcatcg gcgaatcgga ggcgccgacg gtgcgcttgg cctcctggcg
      121 gctgagagtg ccgtcgtcgt cgacgcggat gaaatacagc acggccaccc tggtcgcctt
      181 gatctgctcc atcgggctcg tgatcggctt ggcgaccggt tcgtcggtcg aggggcgagc
      241 cgcctccgga gccgtctcgg gtcgtccggc cggcttcggc tgggccgtct cgccgacggc
      301 cggctcggga gcgacgacga tcggcccctc gcgcccggcc gtcggctcga ggctgccgtc
      361 ccctctggac ggctcgtccc ctcgggacgg ctcgtcaccc gcggcccggt cgtcgcctgg
      421 cttgccgacg gcctctatga acccggtcga ctcgagcgtc tccttgatca cgtcccagtt
      481 gacgacgaac aacaggagcg taacgagcat catgacgagc cagacgaggc agcccgcgcc
      541 cttcttcttg cttggacctc cgcgggtccc cgacttgcgt tgagccatta tgggtcgata
      601 ctagccgcgc tccgcggcgg ggtcaagcgg aggtcaattc gtcgagctga gccctcgcga
      661 gcgcatcgcc cggctccatc tcgagcacga tcctgaagaa cccggccgcc tcctcgtccc
      721 taccctgcct gcgggccacg acgccgaggt tcgagatgat cttgatattc tcgggctcct
      781 tgcgtagcgc ccgctcgagc ctgccccggc tctcggacag catgccgagc tccatctcgc
      841 agatggccaa ttcgttcagg aggtcgacct cgtcggcgcc tagctcgagg gcgcggagga
      901 acgcggcgcg gccatcctcg tagcgtccga ggcgacggtg tccccagccg acgaggaacc
      961 agccgttcca gacaccgtcg ttgccgcggt tgaactccat ggctcgctcg atgccttcct
     1021 cttcccggcc catgcgtatg aaatcatagg cttccttgaa tttcgcgtcg gcgtcgccgc
     1081 ggaccctgag cttcgacagc acctcgcggg ccttggacag cttggcctcg tcatccccca
     1141 tggccgcgta cgactcgaac agcctggcgg cctggccgta ctcccggttc ttgaggtaga
     1201 agaggccggc gttgaagaat gcgtcgggat acgccggctc catggccagg agccgtttat
     1261 aggcctcgaa ggccctggaa cgcatcgcgt ctgccagttc ctccttaccg gaacgatcga
     1321 gggcgtcggc cttgtcctcg tagaggatgg ccaggttcag agtcggctcg ggcgcctcgg
     1381 gtgagaggcc ggcgagcgcc ttgaagatct cctcggccac ctcgagatcg ccgttcctgg
     1441 ccttgagaat gccggcctcg gacagctcgg cgaatagctc cggcttcacg gccgtcacga
     1501 aggcgcgata ataggctccg tcgggattgt ccgggtcgta cgcgagcagg cgtagcatgc
     1561 cggccacgat catctcccag ctcaggtcgg ataggtcggg cgcgccttgg cctagctcca
     1621 cgggtatcgg tatggacggg tcgaccgtga acgagcctat gggcctggcc atgccctcgg
     1681 gtatcctgat gaaggctatg ttgcccggaa ggcgcggcga ttcgtcggta ttcatcgttt
     1741 ctccgtattc ggccccctcg gggccagtcc gctgggtagg gatcaagggg cgggagcgcc
     1801 gccctgatcg tcgtcggcct ggttcttctt ctgtcccagg tagtcgttca ggtgcatctt
     1861 gtaggacatg ggcaccgtcg cctcatcgaa gcgtacggcg atggcggtca gatcctttcg
     1921 gccctcgacg tcctcgtagc ggaccacgga gccggacacg acgacggtct cgcgcggatc
     1981 ctcgaggtcg acgcgcagcc tgcagctctt gccgacgagg aacttggcca gcccgacgat
     2041 gatcaccttg gcgccggaga acgac
//
LOCUS       DAOOLN010000269         2059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_68427_length_2059_cov_2.491018, whole genome shotgun sequence.
ACCESSION   DAOOLN010000269 DAOOLN010000000
VERSION     DAOOLN010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68427_length_2059_cov_2.491018"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            385..1770
                     /gene="dnaA"
                     /locus_tag="PLW80_04285"
     CDS             385..1770
                     /gene="dnaA"
                     /locus_tag="PLW80_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPB65754.1"
                     /translation="MNSWDYQVFWNEAMSQFSNELSEQEFSMWFNIEYDSSTQDSILV
                     RVPSAFYRDQLAKSYQKAIESKLFELSGKRLTIEFVVVKPDSSTSVPKPAPERRQETK
                     PSKAQAEPEKPREKHPQLNLAHTFENYIIGENNSFAANAAMAIAANPGTAYNPFLIYG
                     DVGLGKTHLMQAIGNRAWSLNPKLKIICVSAENFTNDFIHSIHEKAMSSFKNRYRTVD
                     ILLIDDIHFFENKEGSQEELFHTFNALYDGNKQMVFTCDRPASELKNLSDRLRSRFER
                     GLNVDLQPPNYETRYAILKKKVETNNLNIPPEVFDVIARNISTNVRDLEAALVKLNAY
                     ADLVGKEITLEVAQQQLKDVFSQTRHGNVTIDHIIRTVSEAYKVSANDLKGKKRTRSI
                     VVPRQIAMYIAREITEYSTTELGLEFGGRDHTTVMHSCQKIDEQLKLDPMLDAKIQKL
                     IQAVRQMSVKS"
BASE COUNT          542 a          576 c          530 g          411 t
ORIGIN      
        1 gcccgaaagc agcgactgcg ccgagcgtgc ctgcacgtgc gcagtatatc ttcgatgcgc
       61 caaaaagtaa aggatacggg cctatatata gaaggaagcg ctgctgtaaa ccatatgtgc
      121 aaaacttgtg cagaatctgg tattcctcta tcagatatag aaaagacgta tatcggagca
      181 aaggcgctaa acctgagcgg aaacggcata gcaatactat cttattatat ataaaggact
      241 tatatacatc cactacatgg atttttagta gattctgcag ggttggcttc ttttttttag
      301 atttgacgag tgatatactc cacccatcct ttgtgataga ttgtggaaaa gttgttggaa
      361 actaaatcgg gagatacgta cacgatgaac agctgggatt atcaagtctt ctggaacgag
      421 gcgatgagcc aattctccaa cgagttgagc gaacaggaat tctcgatgtg gttcaatatc
      481 gagtacgact cttcgaccca agactcgatc ctcgtgcggg taccgtcagc tttttataga
      541 gatcagctgg caaagtcgta ccagaaggcc atcgaatcca agctgttcga gctttcgggt
      601 aagcgcctga ccatagaatt tgtcgtggtc aagccggatt catcgacctc tgtccccaag
      661 cccgctccgg agcggcggca ggaaacgaag ccgtcgaagg cccaggctga acccgaaaag
      721 cctcgggaga aacatccaca gctcaacctg gcccatacct tcgagaacta catcatcggc
      781 gagaacaact cgttcgcggc gaacgcagcc atggccatcg cggccaaccc cggcacggcc
      841 tataacccct tcctgatcta cggcgatgta gggctcggca agacgcacct gatgcaggct
      901 ataggcaatc gagcctggtc gctcaacccc aagctcaaga tcatctgcgt ctcggcggag
      961 aatttcacga acgacttcat ccactcgatc cacgagaagg ccatgtcgtc gtttaaaaac
     1021 cgctaccgca ccgtcgatat actcctgatc gacgatatac acttcttcga gaacaaggag
     1081 ggctcccagg aggagctgtt ccataccttc aacgcgctgt acgacggcaa caagcagatg
     1141 gtcttcacct gcgaccggcc ggcctccgag ctcaagaacc tgtccgacag gctacgcagc
     1201 cggttcgagc gcggcctcaa cgtcgacctg cagccgccta actacgagac ccgctacgcc
     1261 atcctcaaga agaaggtcga gacgaacaac ctgaacatcc cgccggaggt cttcgacgtg
     1321 atagccagga acatctcgac gaacgtccgc gacctcgagg cggccctcgt caagctcaac
     1381 gcgtacgcag acctggtggg aaaggagatc accctcgagg tggcccagca gcagctcaag
     1441 gacgtgttca gccagaccag gcacggcaac gtgacgatag accatatcat ccgcacggta
     1501 tcggaggcct acaaggtcag cgccaacgac ctcaagggaa agaagcgcac gaggagcatc
     1561 gtcgtcccca ggcagatcgc catgtacata gctagggaga taaccgagta ttcgacgacc
     1621 gagctcgggc tcgagttcgg cggtcgcgac catacgaccg tcatgcactc atgccagaag
     1681 atagacgagc agctcaagct cgacccgatg ctcgacgcca agatccagaa gctcatccag
     1741 gccgtcaggc agatgagcgt aaaaagctaa gtggttgatg gtcgggggcc ttcctgtaga
     1801 aaagggaggc gtccccgcta tccttcatgc gcgcccgtgg acatgtgcag atccgcgcgc
     1861 gcctatagcc atggttatca acagcccaag tcacggcggg gcatgaaacg aacgggtttt
     1921 tccacttata caggcccctt actatatcta ttacgaattt atataaattc aagttcaata
     1981 aggatgagag cccgaagccc tcatatcctt ttattgcgcg tcatcgcgca tccgtggtat
     2041 tcttagcgga agactcagt
//
LOCUS       DAOOLN010000270         2058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_68475_length_2058_cov_3.149276, whole genome shotgun sequence.
ACCESSION   DAOOLN010000270 DAOOLN010000000
VERSION     DAOOLN010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68475_length_2058_cov_3.149276"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PLW80_04290"
     CDS             <1..670
                     /locus_tag="PLW80_04290"
                     /inference="COORDINATES: protein motif:HMM:NF023367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPB65755.1"
                     /translation="RWYEVLPDGAWPNFTLSNHDQKRHYYRYRSGADSDARARVAAAM
                     LLCLRGTPFLYYGEELGMSCERVPRKALRDPLGLSTWPLPLGRDAERTPMQWDASPNA
                     GFSDAEPWLPVNRDYKDKNVEAQRADPGSLLTWYRRLIRLRSLEPALRIGELRWLDAP
                     AGVMAFRRSFGNREIDVWLNFVARAKTVTTLGGSVLDDPGETIAAGDREIGPYEVLIT
                     AREA"
     gene            671..1069
                     /locus_tag="PLW80_04295"
     CDS             671..1069
                     /locus_tag="PLW80_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPB65756.1"
                     /translation="MDELRIGLTGRKELVVEEWHTASHLGSGGAPVYATPMMVLAMEE
                     AALAAVDHLLGDGQATVGVGLDVRHLAATPLGMRVTATAELVGADGRMLTFRVEARDE
                     VDLIGEGTHVRAVVDLERFRDKLAKKARRA"
     gene            1155..>2058
                     /locus_tag="PLW80_04300"
     CDS             1155..>2058
                     /locus_tag="PLW80_04300"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HPB65757.1"
                     /translation="MADLSVSFLGLKLRNPVLVASSGLTSTKEGVRRAAEAGAGAIVL
                     KSLFEEQLRAEIADIASDAESHPEAEAFLAGMGLSEGEGEYVALVKAAKATTDVPIIA
                     SLNCSGDSLWPELAARVAAAGADAIELNLGVVPMDPATPAAHIEDRLVALVGSVASQV
                     RVPVCSKLGSSYTNVGNLASRLAKAGSRGLTLFNRFYRMDVDLETMSLAAGPTRSSPE
                     AYHESLRWIAALEGRVGAELCASGGVYDGATALRLVAAGAQAVQLCSAVYARGYSAIT
                     SSIEEMGSWLDAHGIARLSELRGRL"
BASE COUNT          326 a          711 c          705 g          316 t
ORIGIN      
        1 gcgatggtac gaggtcttgc ccgacggggc gtggccgaac ttcacgctgt cgaaccacga
       61 ccagaaacgc cattactatc gctaccgctc gggcgccgat tccgacgctc gggcccgcgt
      121 cgccgcggcg atgctcctct gcctgcgcgg cacgcccttc ctctattacg gcgaggagct
      181 cggcatgagc tgcgaacgcg tcccgaggaa ggccctgcgc gacccgctcg gcctgtccac
      241 ctggcccctg cccctcggcc gcgacgccga gcgcaccccg atgcagtggg acgcctcgcc
      301 gaacgccggc ttcagcgacg cggagccctg gctcccggtc aatagggact ataaggacaa
      361 gaacgtggag gcccagcgcg ccgatcccgg ctccctgctt acctggtaca ggcgcctgat
      421 acggctccgc tccctggagc ccgccctgcg gattggcgag ctgcgctggc tcgacgcccc
      481 ggccggcgtg atggccttcc gccgttcgtt cgggaatcgc gagatcgacg tctggctgaa
      541 cttcgtagct cgggccaaga ccgtgacgac cttgggcggt agcgtcctgg acgacccggg
      601 cgagacgata gccgcaggcg accgcgagat cggtccctac gaggtcctga tcacggcgag
      661 ggaggcctag atggatgaac tacggatagg gctgaccggg cgaaaggagc tcgtggtcga
      721 ggagtggcat accgccagcc acctcggctc gggcggcgcg ccggtctacg ccacgccgat
      781 gatggtcctc gccatggagg aggccgccct ggcagcggtc gatcacctgt taggcgacgg
      841 ccaggcgacc gtcggagtcg gcctcgacgt gcgacacctg gcggcaaccc cgctcggcat
      901 gcgcgtcacg gcgaccgccg agctcgtcgg ggctgacggc cgcatgctga cgttccgcgt
      961 cgaggcgcgc gacgaagtcg acctgatcgg cgagggcacc cacgtacggg ccgtcgtcga
     1021 cctggagcgt ttcagggaca agctggccaa gaaggcgcgc cgggcttgat cctttccgga
     1081 tttgccgccg ggccgcgggc tccgtatact gagcatagcg ctaacgcgtc atcttcaatc
     1141 actaaggagc gaccatggcc gatctgagcg tatcattcct cgggctcaag ttgcgtaacc
     1201 ccgtactcgt cgcctcgtcc gggctgactt cgaccaagga aggcgtccgc agggcggccg
     1261 aggccggcgc cggcgccatc gtgctcaagt cactattcga ggagcagctc cgcgccgaga
     1321 tagccgatat cgccagcgac gccgagtcgc atccggaggc cgaggccttc ctggccggca
     1381 tgggcctgag cgagggcgag ggcgagtacg tggcgctcgt caaggccgcc aaggcgacga
     1441 ccgacgttcc gatcatagcc agcctgaact gttctggcga ctcgctctgg ccggagctgg
     1501 ccgcccgcgt cgccgcggcc ggagccgacg cgatcgagct caacctgggc gtcgtaccga
     1561 tggatccggc gactccggcg gcgcacatcg aggataggct ggtagccctc gtcggatccg
     1621 tagcctctca ggtgcgcgtg ccggtatgct ccaagctagg ctcctcgtat accaacgtcg
     1681 gcaacctcgc ctcgcgcctg gccaaggccg gctcgcgcgg cctgacgcta ttcaaccgct
     1741 tttaccgcat ggacgttgac ctggagacga tgagcctcgc ggccggaccg acgcggagca
     1801 gcccggaggc ctaccacgag agcctacgct ggatagccgc cctcgagggc cgcgtcggcg
     1861 ccgagctatg cgcctcgggc ggcgtctacg acggcgccac ggcgctacgt ctcgtcgcgg
     1921 cgggcgccca ggcggtacag ctgtgctcgg cggtctacgc gcgcggctac tcggccatca
     1981 cgagctccat cgaggagatg ggctcctggc tcgacgccca cggcattgcg aggctatcgg
     2041 agctgagggg aaggctgg
//
LOCUS       DAOOLN010000271         2058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_68491_length_2058_cov_2.549675, whole genome shotgun sequence.
ACCESSION   DAOOLN010000271 DAOOLN010000000
VERSION     DAOOLN010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68491_length_2058_cov_2.549675"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PLW80_04305"
     CDS             complement(<1..525)
                     /locus_tag="PLW80_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65758.1"
                     /translation="MLDRPEDFLGRVTFVTGPEKHCGKTTFMNRAALLARRGAAASGR
                     PGPALLTVGYDGEARDLLSGARKPAVPVERGDLIVTTERFARPCSPEILEALPGSTAL
                     GRLCVARAGRPATVALVGPEGNSLVAWAVRRILDEGLGETVLVDGAINRVTQAASIEG
                     ARFVYALRVDAAGLE"
     gene            complement(631..1008)
                     /locus_tag="PLW80_04310"
     CDS             complement(631..1008)
                     /locus_tag="PLW80_04310"
                     /inference="COORDINATES: protein motif:HMM:NF024759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HPB65759.1"
                     /translation="MIIGDDSIGAKVIGCSIAVHRELGPGLLESIYEEALSIELGRSG
                     IPFVRQMELPVIYKGVQLSGAFRVDLLVDNTVVVELKCVDRILPVHEAQVLSYLRLGG
                     WRIGLLINFKSAVLKNGIRRVLL"
     gene            complement(1062..>2058)
                     /locus_tag="PLW80_04315"
     CDS             complement(1062..>2058)
                     /locus_tag="PLW80_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPB65760.1"
                     /translation="EDSLSEDAHSPVPGLVHRYPDRVLLLVTHICSMNCRHCTRRRIV
                     GEEDANMLDHQVDAAIEYIRKTPIVRDVLISGGDPLVLSDDKLERIIKKIREIPHVEM
                     IRIGTRTPVVMPQRITDDLVNMLKKYHPLYINTHFNHPKEITAEAREACRKLADAGIP
                     LGNQSVLLRDVNDCPVLMKQLVQKLLTIRVRPYYIYQCDLSRGISHFRTTVSKGIEII
                     ENLRGHTTGLAVPTFVVDAPGGGGKTPVMPDYLISQNDKRVILRNYEGVITTYDEPDG
                     YHEDCGKCSICSDEPQLNATCGVARLLSGDDKTLEPKNLARAARGGTKFIPRKRK"
BASE COUNT          366 a          624 c          678 g          390 t
ORIGIN      
        1 ctcgaggccg gccgcatcga cgcggagcgc gtagacgaag cgcgccccct ctatggaggc
       61 ggcctgagtc acgcggttga tagcgccgtc gaccagtacg gtctcgccca gaccctcgtc
      121 gaggatgcgc ctgacggccc aggcaaccag agaattgccc tccgggccga ccagggcgac
      181 ggtcgcgggc cggccggccc gggcgacgca gaggcggccg agcgctgtag acccaggcag
      241 cgcctcgagg atctccggcg agcacggcct ggcgaagcgc tccgtggtga cgatgaggtc
      301 cccccgctcg accggcaccg ccggcttgcg ggcgccggac agcaggtcgc gggcctcgcc
      361 gtcgtagccg acggtcagca gggccggccc cggtcgcccg gacgcggccg cgcctcgtcg
      421 tgccaggagc gcggcccggt tcatgaacgt cgtcttgccg cagtgtttct cgggtcccgt
      481 cacgaacgta accctgccaa ggaaatcctc gggcctatcg agcatggcga tgcccttctg
      541 acgaattgaa ccacggagac acggaggcac ggaggatgcg aaacgcttta aggccaaagc
      601 acaccatgat tgtagagctt ggagtacggc tcataatagg accctacgga tcccgttctt
      661 aagtacagcg gatttgaaat tgataagcaa gccgattctc catccaccca agcgtaagta
      721 cgataaaact tgagcctcat gaaccgggag gatcctatca acgcacttca actccaccac
      781 cacggtgttg tcgacaagca aatccaccct gaatgccccc gagagctgga cgcccttata
      841 gataacgggt agctccattt gccgaacgaa gggaatcccg gaacggccga gttcgatact
      901 gagggcttcc tcgtagatag actcaagcaa gcccggtccg agctcacggt gtacggcgat
      961 ggaacaaccg atcaccttag cgcctatcga gtcatcacct atgatcattg cgccatctcc
     1021 gtgactccgt gtctccgtgg ttcgggtttc tcgtatcccg gttacttccg cttgcgcggg
     1081 atgaacttgg taccgcccct ggcggccctg gcgaggttct tgggctcgag cgtcttgtca
     1141 tccccggaca ggagcctggc gacgccgcag gtggcgttga gctggggctc gtcggagcag
     1201 atcgagcact tgccgcagtc ctcgtggtag ccgtccggtt cgtcgtaggt cgtgatgacg
     1261 ccctcgtagt tacgcaggat cacgcgcttg tcgttctgtg aaatcaggta gtccggcatt
     1321 accggagtct tgccgccgcc gccgggtgcg tcgaccacga aggtcggcac cgcgaggccg
     1381 gtcgtatggc cgcgcaggtt ctcgatgatc tcgatgccct tggacaccgt ggtgcggaaa
     1441 tggctgatgc cgcgcgacag gtcgcactgg tagatgtagt acgggcgcac gcggatggtc
     1501 aggagtttct gcacgagctg tttcatgaga acggggcagt cgttgacgtc tcgtagcagc
     1561 acgctctggt tcccgagggg gatgccggcg tcggccagct tgcggcaggc ctcgcgggcc
     1621 tcggcggtga tctccttggg atggttgaaa tgcgtgttga tgtacagcgg gtggtacttc
     1681 ttgagcatgt tcacgaggtc gtcggtgatg cgctggggca tgaccaccgg cgtgcgggta
     1741 ccgatgcgta tcatctccac gtgcgggatc tcgcgtatct tcttgatgat gcgctcgagc
     1801 ttgtcgtcgg acagcacgag ggggtcgccg ccggagatca gcacgtcgcg cacgatcggc
     1861 gtcttccgga tgtactcgat ggccgcgtcg acctggtggt cgagcatgtt cgcgtcctct
     1921 tcgccgacga tgcggcggcg ggtgcagtgg cggcagttca tcgagcagat atgcgtgacg
     1981 aggagcagta cgcggtcggg gtatcggtgc accagccccg gaacaggtga atgcgcgtcc
     2041 tcggaaagcg aatcttcc
//
LOCUS       DAOOLN010000272         2052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_68774_length_2052_cov_2.909865, whole genome shotgun sequence.
ACCESSION   DAOOLN010000272 DAOOLN010000000
VERSION     DAOOLN010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68774_length_2052_cov_2.909865"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PLW80_04320"
     CDS             complement(<1..418)
                     /locus_tag="PLW80_04320"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPB65761.1"
                     /translation="MDARTQVREAADYITARIRVKPTIALVLGSGLGSLANEVEGAVA
                     LPYGDIPHFPSSTAPGHAGRLVVGTMAGKAVLVMQGRFHYYEGHPLERIAFPVRVFKA
                     LGIDTLFLTNAAGGANEGYKPGDFMIIRDHINIMGQN"
     gene            complement(518..1411)
                     /locus_tag="PLW80_04325"
     CDS             complement(518..1411)
                     /locus_tag="PLW80_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 family protein"
                     /protein_id="HPB65762.1"
                     /translation="MEKGKTMDAALAKKVSGFQRDEITGCILYGKLSRVIKDANNAKV
                     LADMAQAERQHYEFWKSLSGKDEAPYRGLVFFYYWCARILGLTFALKLMEKGEKAAID
                     GYRAVAHLVPQAERIGREEEEHEDSLLAMLDEERLAYMGSIVLGLNDALAELTGTLAG
                     LTFALRNGPLVAISGIITGIAAAFSMAASDYLASKADGDPRAAKSALYTGVAYLVTVV
                     LLVMPYLVLPRDGSWIFVSLAITLAIAVAIIAAFNFYLAVAKDLNFKRRFGEMAGISL
                     GVSAFSFGVGLVVRAVFGIDV"
     gene            1673..>2052
                     /locus_tag="PLW80_04330"
     CDS             1673..>2052
                     /locus_tag="PLW80_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65763.1"
                     /translation="MRRMLLAASAACILGASCASLGDPFLVAFGVDSAYQSEALTASG
                     IDEYRERLLGQGDVAAAASIQRLFEAALRYDPNNAEAARYLALVEDFRASRFSSSMAK
                     AEGLLAKPKRNPDDEYAMLLAVRRA"
BASE COUNT          337 a          638 c          687 g          390 t
ORIGIN      
        1 ggttctggcc catgatgttg atgtggtcgc ggatgatcat gaagtcgccg ggcttatagc
       61 cttcgttagc gccgccggcc gcattggtca ggaagagcgt gtctatgccg agcgccttga
      121 agacgcgcac cggaaaggct atgcgctcga gcggatggcc ctcatagtaa tggaagcggc
      181 cctgcatcac gagcacggcc ttgccggcca tcgtgccgac gacgaggcgg cccgcgtggc
      241 cgggggcggt tgacgagggg aaatgcggta tgtcgccata gggcagggcg acagcgcctt
      301 cgacctcgtt ggccaggcta ccgaggcccg agcccaggac cagggctatc gtcggcttga
      361 cccgtatcct cgcggttata tagtcggcgg cttctcgtac ctgcgttctt gcgtccatgt
      421 cgctctcctc gagtggctaa ggtggggcca caggctacca gcgggtccgt ggcctgtcaa
      481 tggcggccag gcctcggtgc cgtggcctcg ggcggcctta tacgtcgatg ccgaacacgg
      541 ccctgacgac gaggccgacg ccgaacgaga acgccgagac gcccaggctg atgcccgcca
      601 tctcgccgaa gcggcgcttg aaattcaggt ccttggcgac ggctaggtag aagttgaacg
      661 ccgcgatgat ggcgacggcg atggcgagcg tgatcgccag gctgacgaag atccaggatc
      721 cgtcccgagg tagcacgagg tagggcatga cgaggaggac gaccgtgacg aggtaggcga
      781 ctccggtgta cagggcggac ttggccgccc tcgggtcgcc gtcggccttg ctggccaggt
      841 agtcggaggc cgccatggag aaggccgccg ctatgcctgt gatgatgccc gatatcgcta
      901 cgagcgggcc gttacgcagc gcgaaggtca ggcctgccag cgtcccggtc agctcggcca
      961 gcgcgtcgtt gaggccgagc acgatggagc ccatgtaggc caggcgttcc tcgtcgagca
     1021 tggccaagag cgaatcctcg tgctcctcct cctcccggcc gatgcgctcg gcctgcggta
     1081 cgaggtgggc caccgcccgg tagccgtcga tcgcggcctt ctcgcccttt tccatcagct
     1141 tgagcgcgaa ggtgaggccg aggatacgcg cgcaccaata atagaaaaat acgagtcccc
     1201 ggtagggcgc ctcgtccttg ccggacagcg atttccagaa ctcgtagtgc tgccgttcgg
     1261 cctgggccat gtcggccagg accttggcgt tgttcgcgtc cttgatgacc ctggaaagct
     1321 tgccgtacag gatgcagccg gttatctcgt cgcgctggaa ccctgagact ttcttagcca
     1381 gggccgcgtc catagtcttg cctttttcca tactatcctc ctttgtttta cggttcttgg
     1441 cgatacccag gcgcttcatg gcgctctgaa gggcgctcgg cctcatgccg agcggctcga
     1501 tggccccgcc cggtcgatcg atgacatgcg tctattgtac ccaaattctg gcctcgagtg
     1561 gaatggaagc cgcggcgcta cgaagtgtat cggtccgggg ctgttggaaa ggcttctcat
     1621 gcacgatact atatcgtgtg tcgtagcgct catgcgctac ccggagtgag ccatgcgtag
     1681 aatgttgcta gccgcctcgg cggcctgtat tctgggcgcc tcgtgcgcgt cgctcggcga
     1741 ccccttcctc gtcgcgttcg gcgtcgattc ggcgtaccaa tcggaggccc tgacggcgtc
     1801 gggcatcgac gagtaccgag agcgcctctt aggccagggc gacgtggcgg ccgcggcgtc
     1861 gatccagcgc ctgttcgagg ccgcgttgcg ctacgatccg aacaacgccg aggccgcgag
     1921 gtacctggcc ctcgtcgagg atttcagggc cagtcgcttc tccagctcga tggccaaggc
     1981 ggagggcctg ctcgccaagc ctaagcgcaa ccccgacgac gagtacgcga tgctcctggc
     2041 ggtcaggcgg gc
//
LOCUS       DAOOLN010000273         2049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_68930_length_2049_cov_3.170010, whole genome shotgun sequence.
ACCESSION   DAOOLN010000273 DAOOLN010000000
VERSION     DAOOLN010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68930_length_2049_cov_3.170010"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="PLW80_04335"
     CDS             <1..1101
                     /locus_tag="PLW80_04335"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPB65764.1"
                     /translation="LTTVKTVIISFLFLYASVFVTYLFGRGIVFPILFLRMSVNAIAT
                     RLSSMVKGSERVSADGLRYDDRVATKDEIKKLSLEIGNMAAVIRGVVPYISASTLRHS
                     EREKPTTERRELAFLFTDIRGFTTICEGRSPEEVVAMLNHYLDIQTEAIVANGGDIDK
                     FVGDEVMAVFDGPEKELQACRAGMAIRKAMAEAQEQARKESGAVVSIGIGINAGPVVF
                     GSVGARERMDFTSIGDTVNLAARLEGANKTYGTKSLVAEAVYDRVKDDFLCREVDLLA
                     VKGKNVPVRIYEVLQERDKASQKLIHIKEGFEAGLAAYRSKRWSAAKKIFAKVSEAYG
                     DEASEVFLRRVEVFEAAPPPADWDGVFRMTVK"
     gene            1262..>2049
                     /locus_tag="PLW80_04340"
     CDS             1262..>2049
                     /locus_tag="PLW80_04340"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPB65765.1"
                     /translation="MLHSEFESLGLMPACLDALRHEGYEKPTPIQSASIPSLLAGRDL
                     LGCAQTGTGKTAAFALPTIQRLTTAANKRQPRKARSLVVAPTRELAIQIDASFAAYGR
                     KTELKRACVYGGVGKAPQIKAMERGVDVLVATPGRLLDLKNEGAIDLSAVEIVVFDEA
                     DRMLDMGFIHDVRKIIALLPPKRQTLLFSATMPTEIESLARSLLNDPVRVDIEPEKPA
                     VELIAQSLYYVEKGCKRDLLVHLIKELGVSRAVVFSRTKHGADR"
BASE COUNT          378 a          673 c          657 g          341 t
ORIGIN      
        1 ctgacgaccg tcaagaccgt gatcatatcg ttcctgttcc tgtacgcgtc ggtcttcgtc
       61 acctacctgt tcgggcgggg catcgtgttc ccgatcctgt tcctgcgcat gagcgtcaac
      121 gccatagcga cgaggctgtc gtcgatggtc aagggctccg agcgcgtctc ggccgacggc
      181 ctgcgctacg acgaccgcgt cgccaccaag gacgagatca agaagctgtc gctcgagatc
      241 ggcaacatgg ccgcggtcat acgcggcgtc gtgccgtaca tctccgcgtc gacgctccgc
      301 cattccgagc gggagaagcc gacgaccgag cggcgcgagc tggccttcct attcaccgat
      361 atccgcggct ttacgaccat ctgcgagggg cgctccccgg aggaggtcgt cgcgatgctc
      421 aaccactacc tcgacatcca gaccgaggcc atcgtcgcca acggcggcga catcgacaag
      481 ttcgtgggtg acgaggtgat ggccgtgttc gacggcccgg agaaggagct gcaggcctgc
      541 cgcgccggca tggccatccg caaggcgatg gccgaggccc aggagcaggc gcgcaaggaa
      601 tcgggggccg tcgtgtccat cggcataggc atcaacgcgg ggccggtcgt cttcggctcg
      661 gtgggcgcgc gggagcgtat ggactttacc agcatcggcg acacggtcaa cctcgcggcc
      721 aggctcgagg gcgcgaacaa gacctacggc accaagtctc tcgtcgcgga ggccgtctac
      781 gatcgggtca aggacgactt cctgtgccgg gaggtggacc tgctggcggt caagggcaag
      841 aatgtgccgg tcaggatcta cgaggtgctc caggagaggg ataaggcctc ccagaagctc
      901 atccatatca aggaaggctt cgaggctggc ctcgcggcgt atcgctcgaa gcgttggtcc
      961 gcggcgaaaa agatattcgc caaggtaagc gaagcgtatg gcgacgaggc gagcgaggtg
     1021 ttcctgcgcc gcgtcgaggt cttcgaggcg gcccctccgc cggccgactg ggacggcgtg
     1081 ttcaggatga cggtcaagta gacggctgcc cggcctgaaa ccgggctatc cgttcgcgat
     1141 agtttctgct atattcatac ggacgagaat atccgcggcc ccgtaaaagg aacgtacgcg
     1201 gaccggtagc gatagacgcg ttccatcgac cgaaaccttc tttctcgccc taaggatatc
     1261 catgcttcat tccgaattcg aatccctcgg cctcatgccc gcctgcctgg acgcgttgcg
     1321 ccatgaaggc tacgagaaac cgacgccgat ccagagcgct tcgatcccga gcctcctggc
     1381 cgggcgcgac ttgctcggct gcgcccagac cggtaccggc aagaccgccg cgttcgcgct
     1441 gcctaccatc cagcgcctga cgaccgcggc gaataagcgg cagccgcgca aggctcgttc
     1501 cctcgtcgtg gcccccacgc gcgagctggc catccagatc gatgccagct tcgccgccta
     1561 cggccgcaag accgagctca agcgcgcctg cgtctacggc ggcgtcggca aggcgcccca
     1621 gatcaaggcg atggagcgcg gcgtcgatgt gctcgtcgcc acgcccggcc gcctcctcga
     1681 tcttaagaac gagggcgcga tcgacctgtc ggccgtcgag atcgtcgtgt tcgacgaggc
     1741 ggaccgcatg ctcgacatgg gcttcatcca cgatgtgcgc aagatcatcg cgctcctgcc
     1801 gccgaagcgc cagaccctgc tgttctcggc gaccatgccg accgagatcg agtcgctggc
     1861 gcgcagcctg ctcaacgacc ccgtccgtgt cgacatagag ccggagaaac cggccgtcga
     1921 gctgatcgcg cagagcctgt actacgtcga gaaaggctgc aagcgcgacc tgctcgtcca
     1981 cctgatcaag gaactcggcg tctccagggc ggtcgtgttc tcaaggacca agcacggggc
     2041 cgaccgcat
//
LOCUS       DAOOLN010000274         2049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_68934_length_2049_cov_3.028586, whole genome shotgun sequence.
ACCESSION   DAOOLN010000274 DAOOLN010000000
VERSION     DAOOLN010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68934_length_2049_cov_3.028586"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1639
                     /locus_tag="PLW80_04345"
     CDS             <1..1639
                     /locus_tag="PLW80_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPB65766.1"
                     /translation="NGSLYLLRDRSMYRIMPQEFFTQAIYSGIVQPGVLVGKAPFPYD
                     PNFDSFWISPDGSKVLLCKDGRNIFLYRLDPDDFGREAAVNAMPYLFLQGNTVVDQVL
                     WPSNDEVTVFTASLREGKRVCGAYRVKAPGVGSESLSAGFQSLDVAGATEISLSPDES
                     RVAIVTPDGIVVRRYSNWAAERTLASPGALHAAWISQDRLAIASSGAIETVGVSDGSR
                     TLVALGQPGRFGWSEAEPGVVVAQTAGGAYKTGALKAAWSRAASFDPRPASTSSPGYR
                     VYSDALASGSYRNTIMVRSAKGLGTRSLLPAPALAYKPFPERDDPRDPLIFDHGSRIR
                     RREAALVFNAYDGAEGLVGVLDTLREYGIEATFFVNGEFIRRNPGAAKLIAESGHEVG
                     NMFFSVFDATDSRYRADAEFIKRGLARTEDEYFVATGDELSLLWHSPHYSTSTEILES
                     AAQMSYTYVGRDVDPLDWVGRFQGSLTQGLYSSAHDLVERAVAAVRPGSIVPIRIGIP
                     DGGREDYLYNELPLLINALLSEGYEIVPVSALMKHAE"
     gene            1663..>2049
                     /locus_tag="PLW80_04350"
     CDS             1663..>2049
                     /locus_tag="PLW80_04350"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB65767.1"
                     /translation="MTEDTMLADSYRLDSQALDYSVSAADAARLDGGAWGDAVIGQPR
                     ALEALAIGTAIRAKGYNVFVTGAVGTGRRTAVMRTLAAYRPARLELRDVAYVYCFRSS
                     TAPRALYFRAGEAAAFKKDLHAFVESV"
BASE COUNT          321 a          766 c          644 g          318 t
ORIGIN      
        1 caacggcagc ctctacctcc tgcgtgaccg gtcgatgtac cgcatcatgc cccaggaatt
       61 cttcacccag gccatctact cgggcatcgt ccaaccgggc gtcctcgtcg gcaaggcccc
      121 cttcccctac gaccccaact tcgactcctt ctggatctcg cccgacggct ccaaggtgct
      181 gctctgcaag gacggccgca acatcttcct gtaccgactg gaccctgacg acttcggccg
      241 agaggccgcg gtcaacgcga tgccgtacct gttcctgcag ggcaacaccg tcgtggacca
      301 ggtgctctgg ccgtcgaacg acgaggtgac cgtgttcacc gcctcgctcc gcgagggcaa
      361 gcgcgtctgc ggcgcctatc gcgtcaaggc ccccggcgtc gggagcgaga gcctgtcggc
      421 cggcttccag tccctcgacg tggccggcgc caccgagatc agcctatcgc cggacgagag
      481 ccgggtagcg atcgtcactc ccgacggcat cgtcgtcagg cgctactcca actgggccgc
      541 cgagcggacg ctggcgtcgc cgggggccct gcacgcggcc tggatatccc aggacaggct
      601 cgcgatagcc tcgtcgggcg ccatcgagac ggtcggcgtt tccgatggct cgaggaccct
      661 cgtcgcgctc ggccagcccg gccgcttcgg ctggtcggaa gccgaacccg gcgtcgtggt
      721 agcccagacc gccggcggcg cctacaagac cggcgccctg aaggccgcct ggtcccgcgc
      781 cgcgtccttc gacccgcgcc cggcttcgac cagttcgccc ggctatcgcg tctactccga
      841 cgcgctggct tccggctcgt accgcaacac catcatggtg cgctcggcca aggggctggg
      901 cacgagaagc ctcctgcccg ccccggccct cgcctacaag cccttccccg agcgcgacga
      961 cccgcgcgat cccctgatct tcgaccacgg ctcgaggatc aggcggcgcg aggcggcgct
     1021 cgtgttcaac gcctacgacg gcgccgaggg actcgtcggc gtgctcgaca cgctgcgcga
     1081 gtacggcatc gaagccacct tcttcgtcaa cggcgaattc atccgccgca acccgggcgc
     1141 cgccaagctc atcgccgaga gcggccacga ggtcggcaac atgttcttca gcgtgttcga
     1201 cgccaccgac tcccgctacc gcgccgacgc cgagttcatc aagcgcggcc tggccaggac
     1261 cgaggacgag tacttcgtcg ccacgggcga cgagctgtcc ctgctatggc actcgccgca
     1321 ctactcgacc agcaccgaga tactggaatc cgcggctcag atgagctata cctacgtcgg
     1381 gagggacgtg gatccactcg actgggtcgg ccgcttccag ggctccctga cccaggggct
     1441 gtattcgagc gcccacgacc tcgtcgagcg cgccgtggcg gcggtcaggc ccggctccat
     1501 cgtcccgata cggataggca tacccgacgg cggccgcgag gactacctct acaacgagct
     1561 accgttgctg atcaacgccc tgctctccga gggctacgag atcgtgccgg tctcggcgtt
     1621 gatgaagcac gcggagtagg ccgcgcggcg cccgatccct gaatgacgga ggacacgatg
     1681 ctcgccgact cgtacaggct cgactcacag gcgctcgact actcggtatc ggcggccgac
     1741 gccgccaggc tggacggcgg agcctggggc gacgccgtca tcggccagcc gcgcgccctc
     1801 gaggcgctcg ccataggcac cgccatccga gccaagggct acaacgtctt cgtgaccggc
     1861 gcggtcggta ccggccggcg tactgcggtc atgcggaccc tggccgccta ccggcccgcc
     1921 aggctcgagc tacgcgacgt cgcctacgtc tactgcttcc gctcatcgac ggctccccgc
     1981 gcgttgtact tcagggcggg cgaggcggcg gcgttcaaga aagacctgca cgccttcgtc
     2041 gagagcgtc
//
LOCUS       DAOOLN010000275         2047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_69043_length_2047_cov_2.371988, whole genome shotgun sequence.
ACCESSION   DAOOLN010000275 DAOOLN010000000
VERSION     DAOOLN010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69043_length_2047_cov_2.371988"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PLW80_04355"
     CDS             complement(<1..249)
                     /locus_tag="PLW80_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HPB65768.1"
                     /translation="MHDPDSLLRLPRSREGQELLARWYGGSRVSDQRDRWERLAESTF
                     ALFDPREATFVSSPGRTELGGNHTDHNDGRVLCASVHLD"
     gene            complement(412..606)
                     /locus_tag="PLW80_04360"
     CDS             complement(412..606)
                     /locus_tag="PLW80_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65769.1"
                     /translation="MKTFAIKSTDRAPSVLELVGEIEDGYVVRIVRHRDDWEDVSEEF
                     MTRELFDTCMRTGYIYEMSA"
     gene            818..>2047
                     /locus_tag="PLW80_04365"
     CDS             818..>2047
                     /locus_tag="PLW80_04365"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPB65770.1"
                     /translation="MPSLTFSDFGFSAPVLEALARKGFEEPTPIQSLAIPVLLSGSSH
                     LVARARTGTGKTAAFGLPLVERLSTPGPKVRALVLVPTRELAVQVSGEIASFAPGPAP
                     RIATVYGGASMGEQLRRLSRGVDIVVGTPGRVLDHIDRKTLDLSALEWLVLDEADEML
                     DMGFIEDIEKVMDASNPARRVVLFSATMPHPIVRIVRDRLGSFETVEDSTNPVQAGLT
                     DQIWLEVRSRDKLEALCRIVDAEDEFYGLVFCHTKIETDSVARALSERGYAAEALHGD
                     VSQDMRERTLARFRDKKTLILVATDVAARGIDVERLTHVVNFNLPFDPESYTHRIGRT
                     GRAGNKGTAITFVTPEEYRRLFALRRASGDGLHKGSVPTVGDVIEAKRSRILAKAAGA
                     ARETLGLELKRDDDTMEV"
BASE COUNT          319 a          716 c          651 g          361 t
ORIGIN      
        1 gtcgaggtgg accgacgcgc agagcacgcg gccgtcgtta tggtcggtat ggttgccgcc
       61 tagctcggtg cggcccgggc tcgagacgaa ggtcgcctct cgcgggtcga agagggcgaa
      121 cgtcgactcg gcgagccgct cccatcggtc ccgctggtcc gagacccgcg agcctccgta
      181 ccacctggcc agcagttcct ggccctcgcg cgatcgcggt agccgtaata gcgaatcggg
      241 gtcgtgcacg ttgtctccct gggccggcgc tgcgcgtccg gcgcgttggt ctagagtaga
      301 ggacagcgat agcacgatag cacggtttcg cgccttggga aagcgaaggc cgcccctcgg
      361 ggcggcctcc tacgctggtc gtttacatgg ttcgaggtgt tttacggctc gctaggcgga
      421 catttcatag atatagccgg tgcgcatgca ggtatcgaac agctcccggg tcatgaactc
      481 ctcgctgacg tcttcccagt cgtctcgatg cctgacgatg cgcacgacgt acccgtcttc
      541 gatctcgccg acgagttcga gtaccgaagg cgcgcgatcg gtgctcttta tggcgaatgt
      601 cttcatcttc atcctccgat cgacgctgtt gcgtccttac ctataatgtc ggcgggccgc
      661 gcgcggcggt ttacgcctat ttacgtgccc ggcggtctgg actatcgatc cccgaaacgc
      721 tatagtcgta ccatcccgcg ggacgggctt ccactcgtcg attcgcacct cgccgaagat
      781 tcgcccacga gaccatacgt tccctataag gacaccgatg ccttctctaa ccttctccga
      841 tttcggattc tccgcgcccg tgctcgaggc tctcgcccgc aagggcttcg aggagcccac
      901 cccgatccag tcgctggcca tcccggttct gctctcgggc tcgtcccatc tcgttgcccg
      961 cgccaggacc ggcaccggta agacggccgc cttcggcctt ccgctcgtgg agcgcctgtc
     1021 gacgcccgga cccaaggtcc gcgccctcgt cctcgtgccg acccgcgagc tggccgtcca
     1081 ggtgtccggc gagatagcca gcttcgcccc ggggccggcg ccgcgcatcg ccacggtcta
     1141 cggcggcgcg tcgatgggcg agcagctcag gcgcctgtcg cgcggcgtcg acatcgtcgt
     1201 cggcaccccc ggccgcgtgc tcgaccatat cgaccgcaag accctcgacc tgtcggcgct
     1261 cgagtggctc gtactcgacg aggccgacga gatgctggac atgggtttca tcgaggacat
     1321 cgagaaggtg atggacgcgt ccaacccggc gcgccgcgtc gtgctgttct ccgcgacgat
     1381 gccccacccg atagtgcgga tcgtccgcga ccggctcggc tccttcgaga cggtcgagga
     1441 ctcgaccaac ccggtgcagg ccggcctgac cgaccagatc tggctcgagg tccgctcccg
     1501 cgacaagctc gaggccctct gccgtatcgt cgacgccgag gacgagttct acggcctggt
     1561 gttctgccat accaagatag agaccgattc ggtcgcccgc gccctgtcgg agcgcggcta
     1621 cgcggccgag gccctgcacg gcgacgtgtc ccaggacatg cgcgagcgca ccctcgcccg
     1681 tttccgcgat aagaagacgc tgatcctggt ggcgaccgac gtggccgcgc gaggcatcga
     1741 cgtcgagcgg ctgacccacg tggtcaactt caacctgccc ttcgacccgg agtcgtatac
     1801 gcaccgcatc ggccgcacgg gccgggccgg caacaagggc accgccataa ccttcgtgac
     1861 gccggaggag taccgtcgcc tgttcgccct gaggcgcgcc tcgggcgacg gcctccacaa
     1921 gggtagcgtg ccgaccgtcg gcgacgtgat cgaggccaag cgctcgcgca tcctggccaa
     1981 ggcggccgga gcggcccgcg agaccctcgg gctcgaactg aagcgcgacg acgataccat
     2041 ggaagtg
//
LOCUS       DAOOLN010000276         2044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_69185_length_2044_cov_3.006033, whole genome shotgun sequence.
ACCESSION   DAOOLN010000276 DAOOLN010000000
VERSION     DAOOLN010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69185_length_2044_cov_3.006033"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PLW80_04370"
     CDS             <1..240
                     /locus_tag="PLW80_04370"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPB65771.1"
                     /translation="LSNRPLVLPGGESLELYVERGQRERIILTVDGQEFYDLQEGDRV
                     SFRRSAFKARLFRHERAAFFQVLRSKLNWSGGPDA"
     gene            233..1927
                     /gene="recN"
                     /locus_tag="PLW80_04375"
     CDS             233..1927
                     /gene="recN"
                     /locus_tag="PLW80_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPB65772.1"
                     /translation="MLEELSIRDFAIIDRLSARFEPGLNLLTGETGAGKSILIGAMGF
                     ILGAKSDTGIIRSGAEETLVTAVVSVDGNRDALAWLSERGIEPEDGAVMVRRGLKRSG
                     RGSAYIQNVPVVRQDLQDFSSTLVELHGQRDGHALLKKDRHRQLIDRYAGIDGEVAEY
                     GEAYVELSAKRKALETMAGSEAERAREIEFLRFMIEEIDAAAPRAGEELELEEEERRL
                     SQHEKLFSAVSVARELLSEADGAVARLRKARQQFEIASAIDQRLADQARRLDDAYYEL
                     EDLGLSLATYADQLLFDPARLEEIESRLSLLQKLKRKYGDDLAAVLARRTESAERLAS
                     LENWEEDRAGLERQVAEIEADVNRRAELLTSARKEASVRLERAVVGVIATLGMPNARF
                     VVRIERFPPAGGKVVVGPTGADDLEFYFSANKGEPLRPLADVASGGELSRFMLALKTV
                     LAEDGGAPTMVFDEIDAGIGGEVALGVGSHLQRLAASRQVLCITHLASIAARADNHLM
                     VEKRVEGDRTVTRLSRLDGQGRVREVSRMLSGDAGSRASLDHAAELVAKLGSTRGT"
BASE COUNT          389 a          641 c          694 g          320 t
ORIGIN      
        1 ctatcgaacc gtcccctcgt gctacccggc ggggagagcc tcgaactgta cgtcgagcgc
       61 gggcaacgcg agcgcatcat cctcacggtt gacggccagg agttctacga cctccaggag
      121 ggcgatcgcg tcagcttccg ccggtcggct ttcaaggcgc gactgttccg ccacgagcgc
      181 gcggcgttct tccaggtcct gaggtcgaag ctgaattggt cgggaggccc cgatgcttga
      241 ggaactgtcc atccgcgatt tcgccatcat cgaccggctg tcggctcgct tcgagccggg
      301 cctaaatcta ctgaccggag agaccggcgc cggcaagtcc atcctgatag gcgcgatggg
      361 cttcatcctg ggggccaagt cagataccgg gatcatacgc tccggcgccg aagaaactct
      421 cgtgacggcc gtcgtgtcgg tagacggcaa ccgggacgcg ctcgcttggc tgtcagagcg
      481 gggcatagaa cccgaggacg gcgcggtcat ggtacgccgc ggccttaagc gctccggaag
      541 gggttcggcc tatatccaga acgtacccgt cgtcaggcag gatctgcagg acttctcgtc
      601 gactctcgtc gagctgcacg gccagcggga cggccacgcc ctactcaaga aggaccgcca
      661 ccgacagctg atagaccgct atgcgggcat cgatggcgag gtggcggaat acggagaggc
      721 ctacgtcgag ctgagcgcca agcgcaaggc cctggagacg atggcgggct cagaggcgga
      781 gcgggcgcgt gagatagaat ttcttcgctt tatgatagaa gagatcgacg cggctgcgcc
      841 tcgggccgga gaagagctcg agctcgagga agaggagcgt cgcctctccc agcacgagaa
      901 actgttctcc gccgtctcgg tagcgcgcga gctcttatcg gaggccgatg gcgccgtagc
      961 caggctcagg aaggccagac aacaattcga gatcgccagc gccatcgacc agcgcctcgc
     1021 cgaccaggcc agacgcctcg acgacgcgta ctacgagctg gaagacctgg ggctgtcgct
     1081 ggctacctac gcggatcagc tcctgtttga cccggcccgt ctcgaggaga tagaatccag
     1141 gctatcgctg ctacagaagc tgaagcgcaa gtatggcgac gacctggcgg ccgtgctcgc
     1201 ccgcaggacc gaatcggcgg agcggctcgc cagcctcgag aactgggaag aggatagggc
     1261 gggcctcgag cgccaggtcg ccgagataga ggcggacgtg aaccgacgcg ctgagctcct
     1321 gacgagcgcc aggaaagagg cctccgtacg gctcgaacgg gctgtggtag gcgtcatcgc
     1381 gaccctgggc atgccgaacg cccgcttcgt cgtgaggatc gagcgtttcc ctcccgcggg
     1441 cggcaaggtc gtcgtcgggc cgaccggcgc cgacgacctg gaattctact tctccgccaa
     1501 caagggcgag ccgctgcggc cgctggccga tgtggcgtcc gggggcgagc tatcccgttt
     1561 catgctggcc ctgaagaccg tcctcgccga ggacggaggc gctccgacca tggttttcga
     1621 cgagatcgac gccggcatcg gcggcgaggt cgcgctcggc gtcggttcgc acctgcaacg
     1681 gctcgccgcc tcgaggcagg ttctttgcat tacccatctc gcgtcgatag ccgctcgcgc
     1741 cgataatcat cttatggtgg agaagcgcgt ggagggcgac cgcacggtga cccggctctc
     1801 gcggctcgac ggccagggcc gcgtgaggga ggtctcgcgc atgctatcgg gcgacgcagg
     1861 gtcacgggcg tcgctcgacc acgcggccga actggtggcc aagctcggta gcactcgagg
     1921 aacgtaatgc caaagataac gcaggaacag aggaacaggt acgccgcgaa ggtcaaggaa
     1981 tacaaggcga cggccgacgg catcctcgca aaggagcagt ccctgctcaa cgtcctgaag
     2041 cagc
//
LOCUS       DAOOLN010000277         2041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_69325_length_2041_cov_3.124371, whole genome shotgun sequence.
ACCESSION   DAOOLN010000277 DAOOLN010000000
VERSION     DAOOLN010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69325_length_2041_cov_3.124371"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PLW80_04380"
     CDS             complement(<1..341)
                     /locus_tag="PLW80_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65773.1"
                     /translation="MTVRAFAAVALMAAALMAAVASQPLYAQGAAAQSAAPTDGAAAE
                     VLFRFPVGGVVSSGPTVAAGRVWLLSDSRTLYTLTVDGVAIGKRGLSERRAAFIVGDM
                     LGQVFQKRFKKR"
     gene            complement(358..1515)
                     /locus_tag="PLW80_04385"
     CDS             complement(358..1515)
                     /locus_tag="PLW80_04385"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HPB65774.1"
                     /translation="MIEAGRVVRVGIERIVPGGDGIGRVDGRACFVPLSAPGDLLEAR
                     VVDSRPDYVRAEIVDLLEPGPGRVEPRCPHYGACGGCNLQHLSYEAQLEAKLGLARDA
                     WLRVGRSAEADFTIEPSAPFGYRNRAQFRADASGRPGYARRGSNELLAFDSCPILAEP
                     LERWARSHTAEPGRRFVAFGRDAEPGEAPGSGRAYIEGVDDEATAVVLGRSFRFATAG
                     FFQSNLAMVERLVPAVRSGFSEGAKGDAAADLYCGVGLFGAFLSGSFRRLVCVEQERR
                     SIGYACVNVPRGASFSAMRIEDWTRSAQARERFDLVVVDPPRSGLGPAVRSWLAEARP
                     SALSYVSCDPVSLARDAGFLIDAGYAVESAALYDFYPQTAHIESHVRFRVD"
     gene            complement(1519..>2041)
                     /locus_tag="PLW80_04390"
     CDS             complement(1519..>2041)
                     /locus_tag="PLW80_04390"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HPB65775.1"
                     /translation="VGVVAVREAVAELLAGNGHRPLSEFMGEPLFVLPNKTVGDLFAL
                     FKKRRETFAVVIDEYGGLAGLVTMRDIVEEIVGEIYEDSDEASRERIERRQDGSYLVS
                     GDAPLSVIAEIAGGEPPNLPYVQTVAGYVAWALDRIAVPGDKAETPLGGFIVTAVDAN
                     RVRTAVFRPAGKD"
BASE COUNT          330 a          716 c          736 g          259 t
ORIGIN      
        1 cgctttttaa atcgtttttg aaaaacctgc ccaagcatgt cgccgacgat gaaggcggcc
       61 cggcgctcgg acagtccccg cttgcctatg gccacgccgt cgacggtcag ggtgtacagg
      121 gtgcgcgaat cggacaggag ccatacgcgg ccggccgcga ccgtcgggcc cgagctgacg
      181 acgccgccga ccgggaagcg gaacaggacc tcggcggcgg ctccgtcggt gggcgcggcc
      241 gactgggccg ccgcgccctg ggcatacagc ggctgagacg cgacggccgc catgagcgcg
      301 gccgccatga gcgcgaccgc ggcgaacgct cgaacggtca tggacctctc ggcggcccta
      361 gtcgaccctg aacctgacat ggctctctat atgcgcggtc tgaggataga agtcgtacag
      421 cgcggcgctc tcgaccgcgt agccagcgtc gataaggaag ccagcgtcgc gggcgagcga
      481 taccgggtcg caggatacgt aggagagcgc ggaagggcgg gcctcggcca gccacgagcg
      541 tacggccggg ccgagccccg agcgcggcgg atcgacaacg acgaggtcga agcgttcgcg
      601 ggcctgggcg gatcgcgtcc agtcctcgat gcgcatggcg gagaacgagg cgccgcgagg
      661 cacgttgacg caggcgtagc ctatcgaccg gcgctcctgc tcgacgcaga cgagcctacg
      721 gaacgatccg gacaggaagg cgccgaacag gccgaccccg cagtacagat cggcagccgc
      781 gtcgcccttg gcgccctccg agaagccgga acgcaccgcc ggcacgagcc gctcgaccat
      841 ggccaggttg ctctggaaga agccggccgt ggcgaagcgg aagctcctgc cgaggacgac
      901 ggccgtagcc tcgtcgtcga cgccctcgat gtaggcgcgg ccggaaccgg gcgcttcgcc
      961 gggctcggcg tcccgcccga atgcgacgaa gcgccgcccc ggctcggccg tatgcgagcg
     1021 cgcccagcgc tcgagcggct cggcgagtat cgggcacgag tcgaaggcga ggagctcgtt
     1081 cgagccgcga cgagcgtagc ctggccggcc cgaggcgtcc gcgcggaact gggcgcggtt
     1141 gcggtacccg aacggggccg atggctcgat cgtgaagtcg gcctcggcgg aacggcctac
     1201 ccgcagccag gcgtcgcggg ctaggccgag cttggcctcg agctgggcct cgtacgacag
     1261 gtgctgcagg ttacagccgc cgcacgcgcc gtaatggggg cagcgcggct cgacgcggcc
     1321 cggccccggc tcgagcaggt cgacgatctc ggcgcggacg taatccggcc gagagtcgac
     1381 gacgcgcgcc tcgagcaagt cgccgggcgc cgatagcggc acgaagcagg cgcggccgtc
     1441 gacgcggccg atgccgtcgc cgccaggcac gatgcgctct atgcctaccc tgacgacgcg
     1501 ccccgcctcg atcacggctc agtccttccc ggccgggcgg aagaccgccg tgcgcacgcg
     1561 gttggcgtcg accgccgtga cgatgaagcc gccgaggggc gtctcggcct tgtcgccggg
     1621 caccgctata cggtcgaggg cccaggcgac gtaccccgcc acggtctgca cgtacggcag
     1681 gttcggcggc tccccgccgg cgatctcggc gatgaccgac agcggggcgt cccccgagac
     1741 caggtaggag ccgtcctgcc gccgctcgat ccgctctctc gacgcctcgt cggaatcctc
     1801 gtagatctcg cccacgatct cctcgacgat atcgcgcatc gtcacgaggc cggccaggcc
     1861 gccgtactcg tcgatcacga cggcgaaggt ctcgcggcgc ttcttgaaga gcgcgaacag
     1921 gtcgccgacg gtcttgttcg gcagcacgaa caggggctcg cccatgaatt ccgacagggg
     1981 cctgtggccg ttgccggcga gtagctcggc gacggcctcg cgcacggcca cgacgccgac
     2041 g
//
LOCUS       DAOOLN010000278         2036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_69546_length_2036_cov_3.034326, whole genome shotgun sequence.
ACCESSION   DAOOLN010000278 DAOOLN010000000
VERSION     DAOOLN010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69546_length_2036_cov_3.034326"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2036)
                     /gene="secA"
                     /locus_tag="PLW80_04395"
     CDS             complement(<1..>2036)
                     /gene="secA"
                     /locus_tag="PLW80_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPB65776.1"
                     /translation="EAFALAREAARRVLGERSFDTQVLGGIVLHDGKIVEMKTGEGKT
                     LSSVAAAYLNSLEGKGVHVVTVNDYLAERDSRWMGSIYSYLGVSVGAILSDMDNDARR
                     AAYAKDITYGTNNEFGFDYLRDNMAMEPSRRVQRGHHYCVVDEIDSILIDEARTPLII
                     SGAAEDDTFKINEVSRLCHHLVEVKKDPETGEYPKEEEGEALDGDYKIDEKSKKVSFT
                     SDGLAKMEDALKKRGLIQGSLFDEQNFEYVHYFTQAVRARELFTKDTEYVVQEGLVQI
                     VDEFTGRILHGRRYSDGLHEAIEAKERIKIARRNRTLATITFQNYFRMYDKIAGMTGT
                     AETESVEFQKIYELDVVVIPTNKPVARLDEDDLVFLNESEKFEAICDEIAEAHAKGQP
                     MLVGTVSIDKSERLSAALTRRGVRHEVLNAKNHAREATIIAEAGAKGAVTIATNMAGR
                     GTDIKLGGNPEYRARKKVGTSADERTYREALEKEYELWRADYEEVKAAGGLYVLGTER
                     HESRRIDNQLRGRSGRLLPGRDELLERPGLPDRRHRDGAPARGIARRHRAPHRARARL
                     RGRQRDRRRALRRPQQRLRPAHRRHAVDAPRERPVHRGGLGGLRRESGVAAARALPGA
                     RVPRGADRELRGRAARAPGPRRQADRAPARERRRVGAGARAGHGAAGGQRLHHGR"
BASE COUNT          273 a          668 c          688 g          407 t
ORIGIN      
        1 cgtccgtgat gcaggcgctg tcctcctgct gcaccgtgac cagcacgtgc tccagcaccc
       61 acgcgtcggc gctctcgcgc aggcgcacga tcagcttgcc gtcggggccc tggcgcgcga
      121 gccgcccgcc cgcgaagctc gcggtcagcg cctcgcggaa cacgtgcgcc aggtagtgct
      181 cgggcggcag ccacgccact ttctcgtcgt agcccgccca gcccaccgcg atgcactggt
      241 cgttcacgtg gcgcgtccac tgcgtggcgt cggtgcgcag gtcgcagacg ctgctgtgga
      301 cggcgtagcg ctcggcgtcg atcgcgttgc cggccacgta gccgagctcg cgcccggtgc
      361 ggcgcacgat gtcggcgagc gattccgcga gcgggcgcgc cgtcgcgatg ccgccggtca
      421 ggaagacctg gtcgctccag cagctcatct cgacctggca gtagccggcc ggatcggccg
      481 cgtagctggt tatcgatgcg cctcgactcg tggcgctcgg ttccgagcac gtacaggcct
      541 ccggcggcct tgacctcctc gtagtcggct cgccatagct cgtactcctt ctcgagcgcc
      601 tcgcggtagg tccgttcgtc ggccgaggtg ccgaccttct tgcgcgcgcg gtactcgggg
      661 ttgccgccga gcttgatgtc ggtaccgcgg ccggccatgt tcgtcgctat ggtcacggcg
      721 cccttggcgc cggcctcggc tataatcgta gcctcgcggg cgtggttttt ggcgttgagg
      781 acctcgtggc gcacgccgcg cctggtgagg gcggcggaga ggcgctcgga cttgtcgatg
      841 ctgacggtgc cgacgagcat cggctggccc ttggcgtggg cctcggctat ctcgtcgcag
      901 atggcctcga atttctccga ctcgttcagg aacacgaggt cgtcctcgtc gagcctggcg
      961 accggcttgt tcgtcgggat cacgacgacg tccagctcgt agatcttctg gaactcgacc
     1021 gactcggtct cggccgtacc cgtcatgccc gctatcttgt cgtacatgcg gaagtagttc
     1081 tggaaggtga tcgtggcgag ggtgcggttg cgcctggcta tcttgatgcg ctccttggcc
     1141 tcgatagcct cgtggagccc gtcggagtat cggcggccgt gcaggatgcg cccggtgaac
     1201 tcgtcgacga tctggacgag gccctcctgg acgacgtact cggtgtcctt cgtgaacagc
     1261 tcgcgggctc ggacggcctg cgtgaagtag tggacgtact cgaaattctg ctcgtcgaac
     1321 agggagccct ggatgaggcc gcgcttcttg agcgcatcct ccatcttggc caggccatcg
     1381 gaggtgaagg ataccttctt gctcttctcg tcgatcttgt agtcgccgtc gagggcctcg
     1441 ccttcctctt ccttggggta ctcgccggtc tcggggtcct tcttgacctc gacgaggtgg
     1501 tggcagaggc gcgaaacctc gttgatcttg aaggtgtcat cctcggcggc acccgagatg
     1561 atcagcggcg tgcgcgcctc gtcgatcagg atcgagtcga tctcgtcgac cacgcagtag
     1621 tggtggccgc gctgaacccg ccgtgacggc tccatggcca tgttgtcgcg caggtagtcg
     1681 aagccgaact cgttattggt gccgtaggtg atgtccttgg cgtaggcggc acgcctcgcg
     1741 tcgttgtcca tgtccgagag gatcgccccg accgagacgc cgaggtagga gtagatcgag
     1801 cccatccagc gcgagtcgcg ctcggccagg tagtcgttga ccgtgacgac gtggacgccc
     1861 ttgccctcga gcgagttcag gtaggcggcg gcgaccgacg acagggtttt gccttcgccg
     1921 gtcttcatct cgacgatctt gccgtcgtgg agcacgatgc cgccgagcac ctgcgtgtcg
     1981 aaggagcgct cgccgagcac acgcctggcc gcctccctgg ccagcgcgaa ggcctc
//
LOCUS       DAOOLN010000279         2033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_69685_length_2033_cov_3.034884, whole genome shotgun sequence.
ACCESSION   DAOOLN010000279 DAOOLN010000000
VERSION     DAOOLN010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69685_length_2033_cov_3.034884"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="PLW80_04400"
     CDS             complement(<1..1321)
                     /locus_tag="PLW80_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB65777.1"
                     /translation="MLKRFLPYYKPYLGLLALDLACALGLGLADLVFPAATRRVVDVI
                     VPAGDMAGLARFGLLLVALYAVRAGLEFILGYYGHVLGVNIQHDLRRDIYRHLHTLDT
                     RFFDDTKTGQIMSRIVGDLFDLAEISHHLPEDLIMAGVRLIGSLVVMLAIDWRLALVV
                     FAIVPAQVLFSSTFRARFRRAFRRNKETMAVINEHIEESVSGVRVVRAFGNEDYERAR
                     FDDGNATFRSSRIESVFHIGVFNTGVSFLSNLGLVIVLVAGGYAAWRGSVSVGAYVAF
                     ALYVARFFQPIEVLVRSMEQFQEGAAGFGRFLEIVDTKPTIVDAPGAIELASVRGDVE
                     FREVSFRYGDGLERVLERVDLRVAAGETVAIVGPSGAGKTTLCSLIPRFYDIEEGSIT
                     VDGLDVREATIASLRQAVGLVQQDVILFSGSVRDNIAYGRLGASDEEV"
     gene            1369..>2033
                     /locus_tag="PLW80_04405"
     CDS             1369..>2033
                     /locus_tag="PLW80_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65778.1"
                     /translation="MILSEPSAPTADDLRKREKELDFFYSLAALLSKPGLDADEAAAG
                     CARLFAAAMGRPEAARVVVRLGDRTATYAGEGAPSDSHDSHDSDGPSPDAMAALAGSR
                     LACGVEASYPGGEQAFSERERSLCSSTAALLAVAAERLEADERERLGRSELERKNAAL
                     SELLSRIELEKAGIRSAMRSAFEERVSPLVAGLAGVAAASGRREEAARLEARIRRELD
                     VMLS"
BASE COUNT          341 a          679 c          723 g          290 t
ORIGIN      
        1 cgacctcctc gtcgctcgcg cccaggcggc cgtaggcgat gttgtcacgg acgctgccgg
       61 agaacaggat cacgtcctgc tgcacgaggc cgaccgcctg cctgagcgag gctatcgtcg
      121 cctcccgtac gtccagcccg tcgaccgtga tcgagccctc ctcgatgtcg tagaagcgtg
      181 gtatcagcga gcagagcgtc gtcttacccg cgccagaagg gccgacaatc gcgaccgtct
      241 cgccggccgc cacgcgcaga tcgacgcgtt ccagcacccg ctcgaggccg tcgccgtagc
      301 ggaagctcac ctcccggaac tcgacgtcgc cgcggacgct cgccagctct atcgcgccgg
      361 gggcgtcgac gatggtcggc ttcgtgtcga ctatctcgag gaagcgcccg aagccggccg
      421 cgccctcctg gaactgctcc atcgagcgca cgagcacctc gatgggctgg aagaagcgcg
      481 ccacgtagag cgcgaaggcc acgtaggcgc cgacgctgac ggagccgcgc caggcggcat
      541 agccgccggc gacgagcacg atgaccagcc cgaggttgga caggaacgac acgccggtgt
      601 tgaacacgcc gatatggaag accgactcta tcctcgagct cctgaacgtg gcgttgccgt
      661 cgtcgaaacg ggcgcgctcg tagtcctcgt tgccgaaggc ccgcacgacg cgcacgcccg
      721 agacgctctc ctcgatgtgc tcgttgatga cggccatcgt ctccttgttg cggcggaacg
      781 cgcggcggaa gcgcgcgcgg aaggtagacg agaacaggac ctgggccggc acgatcgcga
      841 agaccacgag cgccaggcgc cagtctatgg cgagcatcac gacgagcgag cctatgagcc
      901 tcacgccggc catgatcagg tcctcgggca ggtggtgcga tatctcggcc aagtcgaaca
      961 ggtcgccgac gatgcgcgac atgatctggc cggtcttcgt atcgtcgaaa aagcgcgtgt
     1021 cgagggtatg caggtggcgg tagatgtcgc gccggaggtc gtgctggatg ttcacgccga
     1081 gcacgtggcc gtagtagccg aggatgaact cgagcccggc gcggacggcg tacagcgcta
     1141 cgagcaacag gccgaagcgc gcgaggccgg ccatgtcgcc ggccggtacg atcacgtcga
     1201 cgacgcgccg cgtggccgcc gggaagacga gatcggccag cccgagcccc agggcgcagg
     1261 ccaggtcgag ggccaggagg cccagatagg gcttgtagta cgggaggaaa cgcttgagca
     1321 tgatacgaca tggtagcggt tcgggagcct tcgtgtatac tggaacgcat gattctatct
     1381 gaaccgtccg ccccgacggc cgacgacctg cgcaagcgcg agaaggagct ggacttcttc
     1441 tattcgctcg cggcgctcct gtccaagccg ggcctcgacg ccgacgaggc ggccgcaggc
     1501 tgcgcgcggc tcttcgcggc ggcgatgggc cggcccgagg cggcccgcgt ggtcgtcagg
     1561 ctcggcgatc ggacggcgac gtacgctggc gagggcgcgc cgtccgactc gcacgactcg
     1621 cacgactccg acggcccttc gcccgatgcc atggcggcac tcgccggctc gcgcctcgcc
     1681 tgcggcgtcg aggcgagcta cccgggcggg gagcaagcgt tctccgagcg cgagcgctcg
     1741 ctgtgctcgt cgacggcggc cctcctggcc gtggccgccg agcgcctgga agccgacgag
     1801 cgggagcgcc tcggccgttc ggagctggag cggaagaatg ccgccctgtc cgagctccta
     1861 tcgaggatcg agctcgagaa ggccggtatc aggtccgcga tgcgctccgc gttcgaagag
     1921 cgcgtctcgc cactcgtggc cgggctcgcc ggcgtcgcgg cggcttcggg caggcgggag
     1981 gaggcggcca ggctcgaggc caggatacgc agggagctcg atgtgatgct gtc
//
LOCUS       DAOOLN010000280         2031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_69797_length_2031_cov_2.389170, whole genome shotgun sequence.
ACCESSION   DAOOLN010000280 DAOOLN010000000
VERSION     DAOOLN010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69797_length_2031_cov_2.389170"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..325)
                     /locus_tag="PLW80_04410"
     CDS             complement(17..325)
                     /locus_tag="PLW80_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65779.1"
                     /translation="MAVVGAFASAASERLDAAARAAVEAAALAAPVVALAASDLALPA
                     GVGCVTQEADLVSGEVAFGDVIAREPGRAEVVAAAAAAVEAVKAVEAVGEARDEVWDE
                     "
     gene            825..1622
                     /locus_tag="PLW80_04415"
     CDS             825..1622
                     /locus_tag="PLW80_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65780.1"
                     /translation="MSQKRVCIAAALALAATIGAFAAPVVVAFVDGDAQALAGGSWKR
                     LDFDDKLDSAQSVRLAAGAVLELRSQAGSTIAIAAPGTYVIDALFKPKAEASALSTVA
                     GKLEKLAKGGPADQTVAGVRGSEAVVAKGTMWAGGTVEADEAAKAAFEAASVGDHAKA
                     YSLYMDAFQLYVDAFDPTGAARVAYSASLSAIAFGSGARALAALRSASPEDAGALRSS
                     YALALAALSARYGAAPDAKALLKKAIDGGWFDDPASLADAKSLLAGL"
     gene            1743..>2031
                     /locus_tag="PLW80_04420"
     CDS             1743..>2031
                     /locus_tag="PLW80_04420"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPB65781.1"
                     /translation="MDHSDDTPKPIDAHFHADDLYAVAPGFVDEYRELGVLGLASVHD
                     DAGFEATRRIMAGSRAAPVSFGLHPQLPVMDGADALAARAAAGDIAAVGECG"
BASE COUNT          293 a          761 c          686 g          291 t
ORIGIN      
        1 cggtcctcgc gtctcgctac tcatcccaaa cctcatctcg agcttcgcct accgcctcca
       61 ccgccttcac cgcctccacc gcggccgccg cggccgccac gacctccgcc cgcccgggct
      121 cgcgggcgat tacatcgccg aaggcgacct cccccgagac caagtccgcc tcctgggtaa
      181 cgcatccgac gcccgccggc agggccaggt ccgaagcggc gagcgcgacg acgggagcag
      241 ccagggcggc cgcctcgacg gccgcgcgcg ccgccgcgtc gagccgctcc gaggccgcgg
      301 aagcgaaggc tccgacgacc gccacgacgg cgtccgacga gcccgatgct acgccgccgg
      361 cgaccgtggt aaggcactcg actgcccagt ccgttagcgc ggccggcggt tctggcgccg
      421 cggcgacgcc ctccagcgcc gcttcgagcg tggccttcgc cggctcgacg gtcggcggtc
      481 ccgctacggc agcctttagc ggttccgctc aggcggtggc cgacggccca ggcggctcga
      541 atgccgaggc cgcgcctacg acaagcggcc tgggcatgcc atccgacacc ccggacgcgc
      601 ctcgggcgcc cgatggaacg ggctcccgtc ggccggttca ggccgtgatg ccaggcagag
      661 gcggctgcgg acgccgcgac gaggagacgg aaggagacga gcgatagtat cgtaagtctt
      721 ttccagttag ggttttgaaa cggtgtccga tccgcgctat actatgcggg tcgaccgaat
      781 aatcgaccgg ggcggccatg cggcgcctcc gaaaggtgaa gcgtatgagt cagaaacgtg
      841 tatgcatagc ggcggcctta gcgctcgccg ccacgatcgg cgccttcgcc gcgccggtcg
      901 tggtcgcttt cgtcgacggc gacgcccagg ctctcgccgg aggctcgtgg aagaggctcg
      961 acttcgacga taagctcgac tctgcccagt cggtcaggct ggctgccggc gcggtactcg
     1021 agctgcgctc gcaggccggc tcgaccatcg ccatagccgc gccgggcacc tatgtgatcg
     1081 acgccctgtt caagcccaag gccgaagcga gcgccctttc gacggtggcg ggcaagctcg
     1141 agaagctggc caagggcggc ccggccgacc agacagtggc gggcgtacgc ggttccgagg
     1201 ccgtcgtcgc caagggtacc atgtgggccg gcggcacggt cgaggccgac gaggccgcca
     1261 aggccgcctt cgaggcggcg agcgtcggcg atcacgccaa ggcctactcg ctatacatgg
     1321 atgccttcca gctctacgtc gacgcattcg acccgaccgg cgcggcccgc gtggcctaca
     1381 gcgcctcgct atccgctatc gccttcggct cgggcgcccg cgcgctggcc gcgctcaggt
     1441 ccgcctcgcc cgaggacgcc ggagccctgc gcagctcgta cgcgctggcc ttggccgcgc
     1501 tctccgcccg ctacggagcc gcccccgatg ccaaggccct gctcaagaag gccatagacg
     1561 gcggctggtt cgacgacccc gcctcgctgg ccgacgccaa gagcctactc gccgggctct
     1621 agcgttcgag cctccgcctc ggaacgaagg ccgcctccag cccggcggcg gcctttcctt
     1681 tccgcccctc gatgctcgcg gccgtcttcg cgtgccgccg atagtcgtta tactcatcgt
     1741 cgatggacca tagcgacgat acgcccaaac cgatagacgc ccacttccac gcggacgacc
     1801 tgtacgcggt cgcccccggc ttcgtcgacg aataccggga gctcggcgtc ctcggcctcg
     1861 cgtcggtgca cgacgacgcc ggcttcgagg cgacccggcg gatcatggcc ggatcgcgcg
     1921 ccgcgccggt gtcgttcggg ctgcacccgc agctgccggt tatggacggg gcggacgcgc
     1981 tcgccgcccg ggcggcggct ggcgatatcg cggccgtagg cgagtgcggt t
//
LOCUS       DAOOLN010000281         2028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_69950_length_2028_cov_2.439939, whole genome shotgun sequence.
ACCESSION   DAOOLN010000281 DAOOLN010000000
VERSION     DAOOLN010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69950_length_2028_cov_2.439939"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PLW80_04425"
     CDS             complement(<1..438)
                     /locus_tag="PLW80_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65782.1"
                     /translation="MAGNELVVDVNKIKKAAHSAIPLTITTYTLPHEIEVYIEEVLAV
                     FLGELGQARIKDYLVYCLRELAVNAKKANTKRVYFETKGLDLRNPAEYEEGMRTFKAD
                     TMENIAYYLQKQKERQYYIKIIFQARGSNVVLEVRNNVELTRTE"
     gene            628..1842
                     /locus_tag="PLW80_04430"
     CDS             628..1842
                     /locus_tag="PLW80_04430"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPB65783.1"
                     /translation="MKEIVIIRGDDADRRFLQVLLSGRYAVRAFGTVAEAAGAALPSG
                     PRAIVVGSEDCSGGGTVDPIAACARVDAGAGVVCVSSSGGPEGASVPPGGEYRVLGRP
                     LLAEALYAAVRLAVAESTLGVEARRRTAAGQAAAEAATAARIEAAPLSPFGRKLLGEG
                     PAMCKVRERIELYAGNEAPVLIVGESGTGKELAAEAIHLGSRRSARRFLPIDCATLSE
                     SLAESTLFGSVKGAFTDAVDKRGAFESARGGTVFLDEIGELALTVQAKFLRTLESGSG
                     SRVGSVDQRRYDVRIVSATNASSLGDARRFRPELFHRISTLVIEMPALRAHKEDLPLL
                     VGALLAEYASEKHVTADTMAKLWGWDWPGNVRELRNVVRRAALLSGGRETILPADIEF
                     DCGARPWQGCLF"
BASE COUNT          325 a          610 c          693 g          400 t
ORIGIN      
        1 ctcggtccgg gtcagttcga cgttgttgcg tacttcgagg accacgttcg agccgcgggc
       61 ctggaagatt atcttgatgt agtactgccg ctccttctgc ttctggaggt agtaggcgat
      121 gttctccatc gtgtcggcct tgaaggtgcg cattccctcc tcgtactcgg ccgggttcct
      181 gaggtccaag cccttcgtct cgaagtagac gcgcttcgtg ttggctttct tggcgttgac
      241 ggccagctcg cgcaggcagt agaccaggta atccttgatc ctggcttggc ccagctcacc
      301 gaggaacacc gcgaggactt cctcgatgta gacctctatc tcgtggggga gcgtatacgt
      361 ggttatcgta agcggaatcg cgctatgcgc cgccttcttg atcttgttga cgtctacgac
      421 cagttcgttg cctgccatgc ttctctccgt tttcagaata ggacgacgcg cctattagat
      481 tactttatcc cggctagtcg tgcaaccggg ataatcgaag gcgcggcctg ggtggtgcct
      541 cttatgccct tccgggagta tcgtgtaatc aggtatcgaa accttatgga tcattgacag
      601 cctctcggcg cggctagact tagcgatatg aaggaaatag taataattag gggagatgac
      661 gccgatcgga ggttcctgca ggttctattg agcggtcgct acgccgttcg ggctttcggt
      721 actgtcgccg aggccgccgg tgccgcgctg ccttccgggc cgcgagcgat cgtggtcggc
      781 tcggaggact gttccggcgg tggaaccgtc gatccgatcg cggcctgcgc cagagtcgac
      841 gcgggggctg gcgtcgtttg cgtgtcctcg tccggcgggc ccgagggagc gtcggttcct
      901 cctggcggcg agtaccgtgt cttggggcgg ccgttactgg cggaagccct gtacgcggcg
      961 gtcaggctcg ccgtcgccga gtccacgctc ggagtggagg cccggcgtcg gactgccgcg
     1021 ggccaggcgg ccgccgaagc cgccacagcc gctaggatag aggcggcgcc tctgtcccca
     1081 ttcgggcgga agctgctcgg agagggcccc gcgatgtgca aggtccggga gcgcatcgag
     1141 ctctatgccg gcaatgaggc acccgtgctg atcgtcggcg agagcgggac cggtaaggaa
     1201 ctcgccgccg aagcgataca cctaggctcg cgccgtagcg ctaggcggtt cctcccgatc
     1261 gactgcgcca cgctgtccga gagcctcgcc gaatcaaccc tgttcggttc ggtcaaaggc
     1321 gcgttcaccg atgccgtcga taagcgcggg gccttcgagt cggcgcgagg gggcacggtc
     1381 ttccttgacg agataggcga gctggctctc acggttcagg ccaagttcct gcggacgctc
     1441 gagtcgggaa gcggctcgcg cgtaggctcg gtggatcaga ggcgctacga tgtgcggatc
     1501 gtctcggcga cgaacgcctc gagcctgggc gacgccaggc gcttccgccc ggagctgttc
     1561 caccgtatca gcacgctggt gatagagatg ccagcgctca gggcgcataa ggaggacctg
     1621 ccgctgctcg tcggcgccct gctggccgag tacgcgtccg agaagcacgt cacggccgat
     1681 acgatggcca agctatgggg ctgggattgg cctgggaatg tcagggaact caggaacgtc
     1741 gtgaggaggg ccgccctgct atcgggcggc cgggagacga tactgccggc cgacatcgaa
     1801 ttcgactgcg gcgcaaggcc gtggcaaggc tgcctcttct agacgtaggt cggtcggggt
     1861 ggcgtcagga cgcggtcctg gccgatacga cctcgaggta tacggcatag ccgccgtgtg
     1921 gcgacggccc gagggtcggc ttccgcgcga cgaccacggc ccgcttgcct gccgagcctt
     1981 gcaggtacga ccggaacgcc tcgccgcgga acacgatgaa cacccggt
//
LOCUS       DAOOLN010000282         2027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_69979_length_2027_cov_3.188641, whole genome shotgun sequence.
ACCESSION   DAOOLN010000282 DAOOLN010000000
VERSION     DAOOLN010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69979_length_2027_cov_3.188641"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PLW80_04435"
     CDS             <1..592
                     /locus_tag="PLW80_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB65784.1"
                     /translation="AAGVVGPDEGDVLVKGRPLAKWPRRELRSRVMAYLPTDRENRGL
                     SPKASVAMNAVAGRLFDYSFAEFAAGSSPLTDAGRMLGEFGVANWKGRRVDTLSGGNR
                     QRVVASRELSGDAELVVAANPAQGLDRGSRSRLLSRLAAMRDSGSAVLLLSSDPDDAA
                     ELADRSFALYRGRLTPIERTGSFDAAFSEALTGAKP"
     gene            589..1656
                     /locus_tag="PLW80_04440"
     CDS             589..1656
                     /locus_tag="PLW80_04440"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65785.1"
                     /translation="MSIATRCRFRFDTIHDAGGTKTAMKLSAALASAAALAALAAAFL
                     SSEPASALRAFFLAPFRTRTAFLSTLELAAPLAVCALGVIVTFRAGHYSLGGEGQAYA
                     GAFAAALAAPILYESLGPASAPLAILVGALAGAIVSAPSAIGKRLAGADVLLTSILVS
                     QGVIFAVDWAIGGPFRDQSSNLVAMRAIPRGALLPRLAPPSTLTAASIVALAACLIAA
                     FYFERTRSGTAHDLYGRNQRFAELQGFDVARLSWAPIVAAGVLHGAAGAMMALGARGT
                     AIRGMSGGIGWSAIGAALVAGSRPQALPFAALLFAWLDSGSRQAAILSDLPPDAGQAI
                     KAIVILAVSARPAIAALRRRR"
     gene            1653..>2027
                     /locus_tag="PLW80_04445"
     CDS             1653..>2027
                     /locus_tag="PLW80_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB65786.1"
                     /translation="MSALVSLLAGSLAFMAPLAPAAVGGLVTEYVGALNIALEGFILI
                     GAFSYAAVGAVAGPVVGFGAALAITAVAAAGADLFSRKAKADPFVVGLGVNMLAPAAA
                     SIVSQSLFATKGVAAVAGLQSTL"
BASE COUNT          245 a          728 c          711 g          343 t
ORIGIN      
        1 cgccgctggc gtcgtcggcc cggacgaggg agacgtgctg gtcaagggcc ggcccctggc
       61 gaaatggccg aggcgggagc ttcgctcgag ggtgatggcg tatctaccca ccgacaggga
      121 gaaccgcggt ctatccccga aagccagcgt agcgatgaac gccgtggctg gccgcctatt
      181 cgattattcg ttcgccgagt tcgcggccgg ctcgagcccg ttgaccgacg cgggtcgcat
      241 gctcggcgag ttcggcgtgg ccaactggaa gggcaggcgc gtcgacacgc tgtccggcgg
      301 caaccggcag cgcgtggtcg cgtcgcgcga gctgtccgga gacgccgagc tcgtcgtggc
      361 ggctaaccct gcccagggcc tggatcgcgg ctcccgctcg cggctgctct cgcggctggc
      421 ggcgatgcgc gactccggct cggcggtact gctcctgagc tccgaccccg acgacgccgc
      481 cgagctcgcc gaccgctcgt tcgccctgta tagggggcgg ctgacgccga tcgagcgtac
      541 gggctcgttc gacgccgcct tctccgaggc cttgacgggg gcgaagccgt gagcatcgcc
      601 acccgttgcc gattccgttt cgacacgatt catgacgccg gcggaaccaa gaccgctatg
      661 aaattaagcg ccgcgctcgc cagcgcggcc gccctcgcgg ccctggcggc ggcattccta
      721 tcaagcgagc cagcctcggc cctgcgcgct ttcttcttgg cgcccttcag aaccaggacc
      781 gcgttcctgt ccaccttgga gctggccgcc cccctcgccg tctgcgcgct cggcgtgatc
      841 gtcacgttcc gagccggtca ctacagcctc gggggtgagg gccaggccta cgccggagcg
      901 tttgccgcgg ccttggccgc gcccatcttg tacgagtccc tggggccggc ttcggcgccg
      961 ctagcgatcc tggtcggcgc cctggcaggc gccatcgtat cggctccctc ggccataggc
     1021 aagcgcctgg ccggggccga cgtgctcctg acgagcattc tagtctccca aggcgttatc
     1081 ttcgcggtgg attgggccat aggcgggccg ttccgcgacc agtcgagcaa cctggtggcg
     1141 atgcgggcca taccgcgcgg cgccctacta ccgaggctcg ctccgccgtc gaccctgacg
     1201 gcggcttcga tcgtcgccct ggccgcctgc ctcatcgcgg ctttctactt cgagcgtacg
     1261 cggagcggga cggcccacga cctctacgga cgtaaccaac gcttcgccga gctgcagggc
     1321 ttcgacgtcg cgcgcctgtc ctgggcgccg atagtagcgg ccggcgtcct acacggggcg
     1381 gcgggcgcga tgatggcgct cggcgctcgg ggcacggcca tccgcggcat gtcgggcggc
     1441 atcggctgga gcgctatcgg cgcggccctg gtcgccggct cgaggcccca ggccttgccg
     1501 ttcgcggcgc tcctgttcgc ctggctcgat tccggctcga ggcaggcggc tatcctgtcc
     1561 gacttgccgc ccgacgccgg gcaggccatc aaggcgatcg tcatcctggc cgtcagcgcc
     1621 aggccagcga tcgcggcgct gaggcggcgg cgatgagcgc gctcgtatcc ttgctcgccg
     1681 gctcgctggc cttcatggcg ccgctagcgc ctgcggccgt cggaggcctc gtcacggagt
     1741 acgtcggcgc gctgaacatc gctctcgagg gcttcatcct gatcggcgcg ttctcgtacg
     1801 ccgccgtcgg ggccgtcgcc ggcccggtcg tgggcttcgg ggccgcgttg gcgatcacgg
     1861 cggtcgcggc ggccggagcc gacctgttct cgcgcaaggc caaggccgat cccttcgtcg
     1921 tcgggctcgg cgtcaatatg ctggcgccgg ccgcggcgtc gattgtatcc cagtcgctct
     1981 tcgcgaccaa gggcgtggcg gcagtggccg ggctgcaatc gaccctg
//
LOCUS       DAOOLN010000283         2022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_70244_length_2022_cov_3.130656, whole genome shotgun sequence.
ACCESSION   DAOOLN010000283 DAOOLN010000000
VERSION     DAOOLN010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70244_length_2022_cov_3.130656"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..>2022
                     /locus_tag="PLW80_04450"
     CDS             185..>2022
                     /locus_tag="PLW80_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65787.1"
                     /translation="MSARAVSLRYDAELSAREAEWTCDRQELYERWLAWRRAGELLLS
                     RGESDFDAAFERLESEAGSWAERFAEAYSIKAAAWDYAYADSLEAKRDWAARATMAVD
                     GAASEAIVAMLGVDAAAAGRKLTSFAVTGFDFEDSSYGAYERVIGGSGLARLGESLEA
                     WSLSAGGLSAKVRAGLAGGRVWESGSILAAAADFALSANESLAGIQAGIMASELGRSI
                     EKAKVSLVDRIAYANRGFDDDMNGLFVADGAWRRDGTRYGKDVVVHSTVGQAFITERV
                     EVPVFAYFELSPWSLGDTLSEASLRGSGASIARARMASAQAAVEEKGREVFGAGDGRG
                     TFGEHVGTSPERLADANPDDGIECFFSDAGSGELGRLMSALVYWSVVEGRGWAEANKP
                     IYDKDLWDDRDDWLEAPTLREVADVGVSVVTGLATGGIGALAMNLADDMLFGALDGLC
                     GYTSWAEAGLSFGKKAVGSLANYCTAGLFDGAIASVAGLGTLRSGFDAAVLSGARGFT
                     ELASSGLVSSVKLDYEADGSLAGLSIDKDRFVESVFGEGAIAGLVGSMAGAACGSSMD
                     YGGAFEDAVWSGFTDLASIAATESASYATHLAFSSYRGVSGAALFE"
BASE COUNT          332 a          572 c          735 g          383 t
ORIGIN      
        1 cttcatcgcc ggacgcgcct cggaagagga ccttgctctt gcggccaacg ccgccttcgg
       61 gccgggcgcg gcgtccgcca aaacgcatgt atccaatctc gagacggtct tgcgtgaagg
      121 atccctgagc ggcgaatcgg ccgccggttc cgaaggcttt gacgccgctc gtcagtattc
      181 gactttgagc gctcgagccg tatcgcttcg ctacgacgcg gaactgtccg ccagggaggc
      241 tgagtggacc tgcgatcgtc aggagttgta cgagcgatgg ctagcgtggc gacgggcagg
      301 cgaactactg ttgtcgcgcg gggagtctga cttcgatgcg gcgtttgaga ggcttgaatc
      361 cgaggccggg tcctgggccg agcgcttcgc cgaggcgtac tcgatcaagg cggccgcgtg
      421 ggactatgcc tacgccgatt cgctcgaggc caagcgggat tgggcggcca gggcgacgat
      481 ggccgtggat ggagccgcgt ccgaggccat cgttgcgatg ctcggagtcg atgcagcggc
      541 ggccggcagg aagctcacct cgttcgccgt gactggattc gattttgagg atagtagcta
      601 tggagcttac gagcgtgtca tcggcggtag cggcctcgcg aggctgggtg agtcgctcga
      661 ggcctggtcc ctatcggctg gcggcttaag cgccaaggtt agagcgggac tcgccggcgg
      721 tcgcgtttgg gaatcgggaa gtatcctcgc ggcggcggcc gatttcgcgt tgtccgcgaa
      781 cgagagcttg gctggtatac aggccgggat aatggcttcg gaactcgggc gctctatcga
      841 gaaagcgaaa gtatcgctcg tcgacagaat tgcatacgcc aaccgaggct tcgacgacga
      901 tatgaacggc ctgtttgtcg ccgacggcgc ctggaggcgc gacggcacac gctacggcaa
      961 agacgtcgtc gtccactcta ccgtcggaca agcatttata acggaacgcg tcgaggtacc
     1021 cgtgttcgcg tacttcgagc tttcgccctg gagcctaggg gatactcttt cggaggcatc
     1081 gctacgaggg agcggcgcgt cgatagcgcg agcgcgcatg gcgagcgcgc aggccgcggt
     1141 cgaagagaag gggcgtgagg tcttcggggc gggagacggg cgcgggacgt tcggagagca
     1201 cgtcggaaca tcgcccgaac gcttggccga tgccaacccc gatgacggaa ttgagtgttt
     1261 cttctcggat gcgggctcgg gcgagctcgg tcgcttgatg tcggctctcg tctactggtc
     1321 ggtggtcgag ggccggggct gggccgaggc taacaagccg atctacgata aggacctgtg
     1381 ggacgatagg gacgactggc tcgaagcgcc gacgctacgc gaggtcgccg acgtcggcgt
     1441 atcggtcgtc acgggcttgg cgaccggcgg cataggcgcc ttggcgatga acctggccga
     1501 tgacatgctg ttcggcgcct tggatgggct gtgcgggtat acgtcatggg ctgaggccgg
     1561 ccttagcttc gggaagaaag ccgtcgggtc gctggccaat tactgcacgg ccgggctctt
     1621 cgacggggcc atcgcgagcg tagccggcct gggtacgcta cgctcgggat tcgacgccgc
     1681 cgtgttgagc ggcgcgcgcg gcttcaccga gctcgcgtcg tccggcctcg tctcgtcggt
     1741 caagctcgac tacgaggccg atggatccct ggcggggctg agcatcgata aggatcgctt
     1801 cgtggagtct gttttcggag aaggcgcgat cgccggattg gtaggtagta tggcaggagc
     1861 cgcctgcgga tcgtcgatgg actacggcgg cgctttcgag gatgcagtat ggtcaggctt
     1921 taccgacctg gcctcgatcg cggcgaccga gagcgcctca tacgccacgc acctggcctt
     1981 ttcgtcgtac cgcggcgtat ctggggcggc gctgttcgag ga
//
LOCUS       DAOOLN010000284         2014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_70637_length_2014_cov_3.151097, whole genome shotgun sequence.
ACCESSION   DAOOLN010000284 DAOOLN010000000
VERSION     DAOOLN010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70637_length_2014_cov_3.151097"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="PLW80_04455"
     CDS             complement(<1..1003)
                     /locus_tag="PLW80_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65788.1"
                     /translation="METPNPSSRRAGRGLALAAACAACALLVSHPLEAAPTDFYWEAP
                     ARLSKGIGAYPQAIRTSGDVIAIWQENVPTDGGGEAWLSMASYRNDGVVRRDRFAGPF
                     RYEAIGDKAKGERGLSAPALFSADDDGNGRLLVAASAGERRVEVYASDDSGRSFRPLS
                     ALELDRPVVAPRVYSNAAGGWYLFVTRSQVFTRTPAGGGEATTYESLSIFYTRSDDGT
                     SWAPLAPFVGGDLGLDPNFLPTAASVDGTDVVVFQTLSGGDRPSYQLYSTASRDGGLS
                     WSTPRRVTGFVDPVHREAQAPDRFDNQRPHLARIGGSLWLTWERKLLSGPAQVYAARL
                     "
     gene            complement(1009..>2014)
                     /locus_tag="PLW80_04460"
     CDS             complement(1009..>2014)
                     /locus_tag="PLW80_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65789.1"
                     /translation="ELAATILKTENDLVVAETRRLVTGGRNLYLQGEFDRAESTLLQA
                     RARWSTTNSTPEVEVEYWLKLVQTALSVKTGRDIPVTAPLFPEMSQLLSLAKQYYQEG
                     AALLAKRDKTGAIRAFSQAKQKISEVKVVFPLNQEARVLELRIDQLSDPDEFGRSFAR
                     LYNEAKAKIDARADLTTAYSDLQDLEAINPRYPGLRAQIERAEILLGFRQPPPDPKAI
                     AEARSLVAAARRIVDSGQVAQFTFARAQLEKALGLDPNNAEASALKDRIATYIGGDTA
                     IVLPSAAETLYNEAVAFFTAGDYLNARVRLTRLAAVYPRGATMQKVSDLDARLTARGY
                     "
BASE COUNT          286 a          665 c          732 g          331 t
ORIGIN      
        1 cgaggcgggc ggcgtagacc tgggccggac cggacagcag cttgcgttcc caggtcagcc
       61 agagcgagcc gcctatcctg gccaggtgcg gccgctggtt gtcgaagcgg tcgggggcct
      121 gggcctcccg gtgcaccggg tcgacgaagc cggtgacgcg gcgcggcgta gaccaggaca
      181 ggccgccgtc ccgcgaggcg gtcgagtaca gctggtagct cggcctgtcg cctccggata
      241 gcgtctggaa cacgaccacg tccgtcccgt cgacggaggc ggccgtcggg aggaagttcg
      301 ggtcgagacc caagtcgccg ccgacgaagg gggccagagg cgcccacgac gtgccgtcgt
      361 cggagcgcgt atagaagatc gatagcgact cgtaggtcgt cgcctcgccc cctccggcag
      421 gagtcctcgt gaagacctgg cttctcgtga cgaacaggta ccagccgccg gccgcgttcg
      481 aatacacgcg cggcgctacc accggccggt ccagctcgag ggccgacagc gggcggaagg
      541 atcgccccga gtcgtccgag gcgtagacct cgacgcgacg ctcccccgcg gaagccgcga
      601 cgagcagtcg accgttgccg tcgtcgtcgg ccgagaacag ggccggagcg ctcaggccgc
      661 gctcgccctt ggccttgtcg cctatggcct cgtagcggaa cgggccggcg aagcgatcgc
      721 gcctgacgac tccgtcgttc ctataggagg ccatcgacag ccaggcttcg ccaccgccgt
      781 cggtcggcac gttctcctgc cagatcgcta tgacgtcgcc ggaggtcctg atggcttggg
      841 gataagcccc gatgcccttg gacaggcgcg cgggcgcttc ccaatagaag tcggtcgggg
      901 cggcctcgag cggatgcgag acaaggagcg cgcaggcggc gcaggccgcg gccagggcca
      961 gtccgcgtcc cgcgcggcgg gagctcgggt tcggcgtttc catggtcgtc agtagcccct
     1021 cgccgtaagg cgcgcgtcga ggtcggatac cttctgcatc gtcgcgcctc tcggatagac
     1081 ggccgcgagc ctcgtcagcc tgacgcgagc gttcaggtag tcgccggcgg tgaagaaggc
     1141 gaccgcctcg ttgtacagcg tctcggcggc gctcggtagc acgatcgcgg tgtcgccgcc
     1201 gatataggtg gcgatgcggt ctttgagggc gctggcctcg gcgttgttcg gatcgagtcc
     1261 gagcgccttc tcgagctggg cgcgcgcgaa ggtgaactgg gctacctgcc ccgagtcgac
     1321 gatgcgcctg gccgcggcga ccagcgatcg cgcctcggct atcgccttcg ggtccggcgg
     1381 cggctgcctg aagccgagca gtatctcggc ccgctcgatc tgggcgcgca gtcccgggta
     1441 acgcgggttg atcgcctcga ggtcctgcag gtcgctgtac gcggtcgtca gatcggcccg
     1501 cgcgtcgatc ttggccttgg cctcgttgta cagcctcgcg aagctacggc cgaactcgtc
     1561 ggggtcggac agctggtcga tccgcagctc gagcacgcgc gcctcctggt tgagcgggaa
     1621 gaccaccttg acctcggata tcttctgctt ggcctgcgag aaggcgcgga tggcgccggt
     1681 cttgtcccgc ttggcgagca gggcggcgcc ttcctggtag tactgtttgg ccagggatag
     1741 gagctggctc atctccggga agagcggcgc cgtgacgggt atgtcgcggc ccgtcttgac
     1801 cgatagcgcg gtctggacga gcttgagcca gtactcgacc tctacctcgg gcgtcgagtt
     1861 ggtggtgctc cagcgcgcgc gggcctgaag cagcgtcgac tcggcccggt cgaactcgcc
     1921 ctgcaggtag aggttccggc cgccggtcac gaggcgcctc gtctcggcga cgaccaggtc
     1981 gttctcggtc ttcaggatgg tcgcggccag ctcg
//
LOCUS       DAOOLN010000285         2010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_70828_length_2010_cov_3.779540, whole genome shotgun sequence.
ACCESSION   DAOOLN010000285 DAOOLN010000000
VERSION     DAOOLN010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70828_length_2010_cov_3.779540"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="PLW80_04465"
     CDS             <1..1020
                     /locus_tag="PLW80_04465"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HPB65790.1"
                     /translation="TKIDAARESARRGGTRLTAPTLVASLRRVTRETNRSGLVSLFAA
                     FFGAIVAFGLIVWAMERTDPAGGISTPFDGLWWAVVTLATVGYGDTYPASALGKAAAM
                     AFMIGGFVLSAIVSGTVASIFVERRIREGKGLQDIRLKGHFVVCGWNVHADAVLDGLE
                     AESRGAPIVLINGLEPERFDAIRSAHPDVDLRFVRGDHTLESVLRKASVQQCRSCILL
                     PDESGGSGEANADERTILAALAVKSIARDVAVRAAILKAESEQHLRRAEVDDVVLHGE
                     FTGFLSDSSGAIDDFIKRKFAEAAIDLGSGDKPEGKARLAPGPDYVIGGTDVAFVVGG
                     SVTHG"
     gene            1013..1519
                     /locus_tag="PLW80_04470"
     CDS             1013..1519
                     /locus_tag="PLW80_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPB65791.1"
                     /translation="MAKDAELLRRTSLLDGLSSDAVSLVLDSGSFVRFKAGETVMHEG
                     EKSDTMYLFLEGDVDVTKDLTLKISGKGFGHAEKSMTRLRAGTAPVFGEMSMFSDEPR
                     SATVGAASDCVLFEVTRRSFEAICAPDPRLGLELTRRIAVMLASRVRKGNDDVLKLST
                     ALSIALSR"
     gene            1533..>2010
                     /locus_tag="PLW80_04475"
     CDS             1533..>2010
                     /locus_tag="PLW80_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65792.1"
                     /translation="MRRATLFAAIVALMAAAGSAFAQDTPSGEAAVPRSFRGIELGMS
                     MDEVKAALAADGLFAYRGDPDVSLLPRPDESLIEVAGVSFVRRAFFQFYGGRLFVMIM
                     ALNEREMDHYSVFTTLAGKYGKPDSLSPSESVWEDGATRLSVERPLAVKYIDLAVFN"
BASE COUNT          328 a          674 c          676 g          332 t
ORIGIN      
        1 acgaagatcg acgcggcgcg cgaatcggcc aggcgcggcg ggacgcggct cacggcgcct
       61 accctcgtag cgtcgctacg ccgcgtcacc agggagacga accgctccgg cctcgtctcc
      121 ctgttcgccg ccttcttcgg cgccatcgtc gccttcggcc tgatcgtctg ggccatggag
      181 cgcaccgatc cggccggcgg catctcgaca ccgtttgacg gtctgtggtg ggcggtcgtg
      241 acgctggcga ccgtaggcta tggagacacg tacccggcga gcgccctcgg caaggcggcg
      301 gcgatggcgt tcatgatagg cggcttcgtg ctgagcgcca tcgtctcggg taccgtggcc
      361 tcgatcttcg tcgagcgccg catcagggag ggcaagggct tgcaggacat acggctcaag
      421 gggcatttcg tcgtctgtgg ctggaacgtc catgccgacg cggtgctcga cggactcgag
      481 gccgagtctc gcggcgcgcc catagtgctg atcaacgggc tcgagcccga gcgcttcgac
      541 gccatcaggt cggcccaccc ggacgtggac ctcaggttcg tccgcggcga ccataccctg
      601 gagtcggtgc tccgcaaagc ctcggtccag cagtgccgct cgtgcatact cctgcccgac
      661 gaatcgggcg gttccgggga agccaacgcc gacgaacgca cgatactcgc ggccctggcc
      721 gtcaagtcga tagccaggga cgtcgccgtg cgcgccgcca tactcaaggc ggagagcgag
      781 cagcacctca ggcgggccga ggtcgacgac gtggtgctcc acggggagtt caccggcttc
      841 ctctccgaca gctccggcgc catcgacgac ttcatcaagc gcaaattcgc cgaggcggcg
      901 atagatctag gctctggcga caagcccgag ggcaaggccc gcctcgcgcc aggccccgac
      961 tacgtgatcg gcggcaccga cgtggccttc gtcgtcggcg gaagcgtcac ccatggctaa
     1021 ggacgccgaa ctactgcgca ggacctcact cctcgacgga ctatcctccg acgccgtctc
     1081 cctcgtgctg gactccggta gcttcgtgcg cttcaaggcg ggcgagacgg tgatgcacga
     1141 gggcgagaaa tcggatacca tgtacctgtt cctcgagggc gacgtcgacg tgacgaagga
     1201 tctgacgctc aagatctccg gcaagggctt cggccacgcg gagaaatcca tgaccaggct
     1261 cagggccggc accgcgccgg tcttcggcga gatgtcgatg ttcagcgacg agccgcgcag
     1321 cgcgacggtc ggcgcggcca gcgactgcgt gctcttcgag gtgaccaggc gctcgttcga
     1381 ggcgatctgt gctcccgacc cgcggctcgg gctggagctt acccgccgca tcgccgtcat
     1441 gctcgcttcg cgggtccgca agggtaacga cgacgtgctc aagctgtcga cggccctctc
     1501 gatagcgcta tccaggtaac gaggagtccg cgatgagacg agccacccta ttcgcagcga
     1561 tagtagccct gatggcggcc gcaggctcgg ccttcgccca ggacactccg tcgggcgagg
     1621 cggccgtgcc gcgttccttc aggggcatcg agcttggtat gagcatggac gaggtcaagg
     1681 ccgcgctggc cgccgacggc ctgttcgcgt accgcggcga cccggacgtg agcctccttc
     1741 cgaggccgga cgagagcctg atcgaggtcg ccggcgtatc cttcgtcagg cgcgccttct
     1801 tccagttcta cggaggtcgg ctgttcgtga tgatcatggc cctgaacgag cgcgagatgg
     1861 accactactc ggtcttcacc acgctggcgg gtaagtatgg caagcccgac tcgctgtccc
     1921 cgagcgagag cgtctgggag gacggagcga ccaggctgtc ggtcgagcgg ccgctcgccg
     1981 tcaagtacat cgacctcgcg gtcttcaacg
//
LOCUS       DAOOLN010000286         2009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_70889_length_2009_cov_3.544524, whole genome shotgun sequence.
ACCESSION   DAOOLN010000286 DAOOLN010000000
VERSION     DAOOLN010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70889_length_2009_cov_3.544524"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            549..1739
                     /gene="rsgA"
                     /locus_tag="PLW80_04480"
     CDS             549..1739
                     /gene="rsgA"
                     /locus_tag="PLW80_04480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPB65793.1"
                     /translation="MAGLWPAARGMLPARVVGRDRGAYRVVAPDFSGRPGFEAAAVSA
                     AAGRAGAPGEYSGVALSGAYARAIEEADSGDRGRASGVLEYPACGDWVLVDLDGGYPR
                     IQATLGRLSAVARGRAGRTSEAQVIAANIDTLIVVLALDGGRNFLPRLAERALVLARA
                     SGAAPVLALNKRDLADEETRLAAEAELSRLAPGVPVLSTSARTGAGIAELAALFGPGE
                     TIALLGKSGMGKSALVNALAGAGPSGAGDVEAAPPPEPGLRAIEGAVRDGDRRGRHTT
                     TASRLYRLDSGLLVVDAPGFRELKVLGDEEATADVFAEVSELAVSCRFSDCTHTNEPG
                     CAVRAALDSGELEPGRFSDYLELEAERASYERRHSARLSASDERRWKLIAKARRRLKD
                     AERG"
     gene            1806..>2009
                     /locus_tag="PLW80_04485"
     CDS             1806..>2009
                     /locus_tag="PLW80_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65794.1"
                     /translation="MALRSFRAAFVTAGLAAIILAAGPAWAQDALPPEGPPPEGAPPS
                     GEAPLEPEDLVLATLALDIASSDY"
BASE COUNT          270 a          665 c          791 g          283 t
ORIGIN      
        1 ggcagcgatt gcggctttag tccgcgagcg ccgaggaagt ccgcgatcgc cgaggaagcc
       61 cgcgatcgcc gaggaagccc gcggcggggg ccgcgtccgg ctcaggcggc cgggcctacg
      121 cgcgtggcgg cccccttcgt tcctgattaa aactatactt tcccttgcga cgcctcgtcg
      181 tcggggcgta cagtacggat aggttcggcg acagccggtc cggccgggcg catagcgccg
      241 gcatccgagc gctcgtccgt aaaggaggct tattatgaag aaggccactg ctaggagcgg
      301 agcggcgttc gggtaggccg cgcgaaccgg accgtgggtc cggctcgtct acagtacaaa
      361 aaacgtagtc ctaccctccg aggcggtcat cgccgcgccc tccccatgcc atcccgtcat
      421 cccgccgcgc gtcaggctcg cggggtaatc gcgcatcgct ggaactcatc gaaaggtaga
      481 ctatgacgaa ggactatagc cccgagggat ggggctggag gcgggagcat cacgcttcct
      541 atgaaactat ggccggcctg tggccggccg cgaggggcat gctgccggcg cgggtcgtcg
      601 ggcgggaccg cggggcctac cgcgtcgtgg cgcctgactt ctccgggcgg ccgggcttcg
      661 aggcggccgc ggtctcggcg gcggctggca gggccggcgc tcccggggag tactcgggcg
      721 tcgcgctgag cggcgcgtac gctcgggcca tcgaggaggc cgactcgggc gaccggggcc
      781 gggcctcggg cgtgctcgag tatccggcct gcggcgactg ggtgctcgtc gacctggacg
      841 ggggctaccc gcgcatccag gcgacgctcg ggcggctgtc ggcggtggcc cgcggccggg
      901 cgggccggac gagcgaggcg caggtgatcg cggcgaatat cgacacgctg atcgtcgtgc
      961 tggcgctcga cggcgggcgg aacttcctgc cgaggctcgc ggagcgggcg ctggtactcg
     1021 cccgggcgtc gggcgcggcg ccggtgctgg cgctcaacaa gcgcgacctg gccgacgagg
     1081 agacgaggct ggccgccgag gcggagctgt cgcgcctggc gccgggggtg ccggtgctgt
     1141 cgacgagcgc gcggaccggg gcggggatag cggagctcgc ggccttgttc gggccaggcg
     1201 agaccatcgc cctgctcggt aagagcggca tgggcaagtc ggcgctggtc aacgcgctcg
     1261 ccggcgccgg gccgtccggg gccggtgacg tcgaggcggc gccgcctccg gagcccgggc
     1321 ttcgggctat cgagggcgcg gtgcgcgacg gcgaccggcg gggccggcac acgacgaccg
     1381 ccagccggct ctaccggctg gactcggggc tgttggtggt cgacgcgccg ggcttccgcg
     1441 agctcaaggt gctcggcgac gaggaggcga cggccgacgt cttcgccgag gtgtccgagc
     1501 tggccgtctc ctgccgcttc tccgactgca cgcacacgaa cgagccgggc tgcgcggtgc
     1561 gcgccgcgct ggattccggc gagctcgagc cggggcgctt ctccgactac ctggagctgg
     1621 aggcggagcg ggcgtcgtac gagcgcaggc actcggccag gctgagcgcg agcgacgagc
     1681 gccgctggaa gctgatcgcc aaggcgcgta ggcggctgaa ggatgcggag cgcggctagg
     1741 gcaggcgagg cgcgtcgtct acattcggta gcaaaataat aggcgccgtt gtatcatgcg
     1801 ccggtatggc actccgatcg ttccgcgccg cgttcgtgac cgcgggcctg gccgcaatca
     1861 tactcgccgc gggacccgcc tgggcccagg acgcgctacc tccggagggg ccgccgcctg
     1921 agggcgcgcc gccctcgggc gaggcgccgt tggagcctga ggatctggtg ctcgcgacgc
     1981 tcgccctcga tattgcgagc tccgactac
//
LOCUS       DAOOLN010000287         2000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_71334_length_2000_cov_2.797429, whole genome shotgun sequence.
ACCESSION   DAOOLN010000287 DAOOLN010000000
VERSION     DAOOLN010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71334_length_2000_cov_2.797429"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="PLW80_04490"
     CDS             complement(<1..1099)
                     /locus_tag="PLW80_04490"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB65795.1"
                     /translation="MENMPAAPSPRRRRRTRIERYRSRFLAFNVLNSFSFMLLSGSIP
                     TLFALDLGASGTYIGLLGSLNFITYFFMPLGRRAIRGRPIIRVFGWSWMLRYWVMAPA
                     IAAPFVAMAGYPSIGLALLLLGSLAFNVFRGVGLIGNNPVLGMMAGERDRGAFLSSVQ
                     ITNGLSAIGTSALTMLALALWSGSPLYAGILALGVASGSIASAILLSLPEPEAYRPDP
                     GSSFAATVREAWADPRLRRFVAVFAPLSFAGGTARTFVVTHASVLYGQSPALVMAYSV
                     AFNLGGVAVGYLSRKLMDRLGAKPLYVLFAVAMALAMAPVAWSPATPSALSTAAYLAL
                     VSFIVGAGVAGQENAGQTYFFSIVKPSHMLDL"
     gene            complement(1132..>2000)
                     /locus_tag="PLW80_04495"
     CDS             complement(1132..>2000)
                     /locus_tag="PLW80_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPB65796.1"
                     /translation="LLVGASLLNIALDLLFVVVFGWGVAGVAWATIISQGASFVGGLV
                     YVAKRNPLVSLKPRSLGFDRDVFKHSVRIGLPTGIQQTMVALGMMALTRLVNGQGTST
                     IAAFAAASRLDTFASMPAMNLSQALSTFTGQNMGAGKTERVKRGHLSASLVGAAISVA
                     VGVAVILFGRGLMGMFTRDAEVMAIGARYLAIVGATYLLFSTMFINNGVMRGAGDAFI
                     PMINTVLALWVVRIPCAVLFSGPLGMGSDGIWWSVPAGWLMGAVFSTWYYLGGRWKRK
                     AVVRAGPEVAGR"
BASE COUNT          381 a          644 c          698 g          277 t
ORIGIN      
        1 cgaggtcgag catgtggctc ggcttgacga tggagaagaa ataggtctgg ccggcgttct
       61 cctggcccgc caccccggct ccgacgatga agctgaccag ggccaggtag gcggcggtgc
      121 tcagggcgct aggcgtcgcc gggctccagg ccaccggcgc catcgccagg gccatcgcca
      181 cggcgaagag cacgtacagg ggcttggctc cgagccggtc catgagcttg cgcgacaggt
      241 agccgaccgc gacgccgccg aggttgaagg cgaccgagta ggccatgacg agggccgggc
      301 tctggccgta gagcacgctg gcgtgggtga cgacgaaggt gcgggcggtg ccgccggcga
      361 acgacagcgg ggcgaacacc gcgacgaagc gcctgagccg cgggtccgcc caggcctcgc
      421 gcacggtcgc cgcgaacgac gatccggggt cgggcctgta ggcctccggc tcgggcagcg
      481 acaggagtat cgccgaggcg atcgagccgg aggccacgcc gagcgccagg atgccggcgt
      541 acaaggggct tccagaccag agtgcgagcg ccagcatggt cagcgcgctc gtgcctatgg
      601 ccgataagcc gttcgtgatc tggacgctcg acaggaaggc cccgcggtcg cgctctcccg
      661 ccatcatgcc gagcaccggg ttgttgccta tcaacccgac gcccctgaag acgttgaagg
      721 cgaggctacc gagcagcagc agggcgagcc ctattgatgg gtagccggcc atggccacga
      781 agggggccgc tatcgccggc gccatgaccc agtaacgcag catccatgac cagccgaaca
      841 ccctgatgat gggcctgccg cgtatggcgc gcctgccgag cggcatgaag aagtaggtaa
      901 tgaagttgag cgacccgagc aggcctatgt aggttccgga cgctccaaga tccaacgcga
      961 acagcgtcgg tatactgccc gatagcagca tgaacgagaa cgaattgagc acattgaagg
     1021 ccaggaaacg ggagcggtag cgctcgatcc tggtccttcg cctccgtcgc ggcgaagggg
     1081 ccgcgggcat attttccatg gatagagtat agagcgtcct aagcggatag gctatctccc
     1141 cgcgacctcg ggacccgccc gtaccacggc ctttcgcttc cagcggccgc ccaggtagta
     1201 ccaggtcgag aacaccgcgc ccatcagcca gccggcgggc acgctccacc agatgccgtc
     1261 cgagcccatg ccgagggggc cggagaacag tacggcgcag gggatacgca cgacccagag
     1321 ggcgagcacc gtgttgatca tcggtatgaa cgcgtcgccg gcgccgcgca tgacgccgtt
     1381 gttgatgaac atcgtcgaga acagcaggta ggtagcgccg actatggcca ggtaacgggc
     1441 gccgatcgcc atcacctcgg cgtcgcgggt gaacatgccc atcagaccgc gcccgaatag
     1501 gatgaccgcg acgcccacgg ccacggagat cgcggcgccg accaggctgg ccgagaggtg
     1561 gccgcgcttg acccgttcgg tcttgccggc ccccatgttc tggcccgtga aggtggacaa
     1621 ggcttggctc aggttcatcg cgggcatcga ggcgaaggtg tccagcctgg aagccgcggc
     1681 gaaggcggca atcgtcgacg tgccctgccc gttgaccagc ctggtcaggg ccatcatgcc
     1741 gagcgccacc atggtctgct ggatgccggt aggcaggccg atgcgcacgc tgtgcttgaa
     1801 tacgtcccgg tcgaagccca ggctcctcgg tttcaggctg accaaggggt tccgcttggc
     1861 cacgtagacg agaccgccga cgaaggaggc gccctgcgag atgatggtgg cccaggccac
     1921 gccggcgacg ccccagccga agacgaccac gaacagcagg tcgagcgcta tgttgagcag
     1981 gctggcgccc acgagcaggt
//
LOCUS       DAOOLN010000288         1999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_71374_length_1999_cov_3.063786, whole genome shotgun sequence.
ACCESSION   DAOOLN010000288 DAOOLN010000000
VERSION     DAOOLN010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71374_length_1999_cov_3.063786"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PLW80_04500"
     CDS             complement(<1..618)
                     /locus_tag="PLW80_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65797.1"
                     /translation="MKAFLSLARLYLQSFYNLPAKKAPGAGRDAKALLKAVGIAALVV
                     IVVGEFGFIFVMLNLGMYEGLAAAGMQGMLILNAVVIATMMTLIVGFMTALSTYYVND
                     MELQLLSMPIKPRAMFGAKFLAVYVSEALFSLFFMGIAMVIFGVKEGPGPLFYLWGLV
                     AALLLPLPPLAAAYIIQIPLMSFARFLKNKRVIVMLGGVLGLVFAL"
     gene            complement(615..1355)
                     /locus_tag="PLW80_04505"
     CDS             complement(615..1355)
                     /locus_tag="PLW80_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB65798.1"
                     /translation="MIELKGISKTYAKSAVKAVDDLSLSIEDGEIFGFLGPNGAGKTT
                     TIKVLTGVLRPDSGRASVDGVDIAVDPLEAKRRIGYVTDNPELFARLKAAEYLNFVGD
                     VYGVDSETRRARVAEYAGLFGIRDALGGSIGSFSHGMKQKLIVTASLLPDPPNWVLDE
                     PMVGLDPKAAFALKEVMRARASAGKTVFFSTHVMEVAERLCDRLAIINKGRIVFVGTL
                     AELKSSHGADGSLESLFLELVDDQGEGA"
     gene            complement(1377..1571)
                     /locus_tag="PLW80_04510"
     CDS             complement(1377..1571)
                     /locus_tag="PLW80_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HPB65799.1"
                     /translation="MSGDKEKDKKQILLRLSPSLWRELAAWAEDDFRSINGQIEFLLT
                     ESVRRRRKGDATDWDDGRDE"
     gene            complement(1584..>1999)
                     /locus_tag="PLW80_04515"
     CDS             complement(1584..>1999)
                     /locus_tag="PLW80_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPB65800.1"
                     /translation="ALRNIVRLYPYDSSDSEGETSLRGSSQEIAEKLRAEIQAKAEAA
                     GIEILEARITHLSYAPEIAAAMLQRQQASAVIAARQMIVDGAVGMVELALDKLKANNI
                     VDLDEERKAAMVSNLLVVLCSNKDVSPIVNSGSIY"
BASE COUNT          347 a          667 c          624 g          361 t
ORIGIN      
        1 gagcgcgaag acgaggccga gcacgccgcc gagcatcacg atgacgcgct tgttcttgag
       61 gaaacgcgcg aacgacatca gaggtatctg aatgatgtac gccgccgcga gcggaggcag
      121 gggcaagagt agcgctgcta cgaggcccca gaggtagaac agcggccccg ggccttcctt
      181 cacgccgaag atgaccatcg cgatgcccat gaagaacagg gagaacagcg cctcggacac
      241 atagaccgct aggaacttgg cgccgaacat cgcccggggc ttgatcggca tggacaggag
      301 ctgtagctcc atgtcgttca cgtagtaggt cgacagcgcg gtcatgaagc cgacgatcag
      361 ggtcatcatc gtcgcgatga ccacggcgtt caggatcagc atgccctgca tgcccgcggc
      421 ggcgaggccc tcgtacatgc ccaggttgag catgacgaag atgaagccga attcgccgac
      481 cacgatgacg accagcgcgg ctatgccgac cgccttgagc agggccttgg catcgcgtcc
      541 ggctccgggc gctttcttgg ccggcaggtt atagaagctc tggaggtaga gccgagcaag
      601 gctcaggaag gctttcatgc cccctccccc tggtcgtcga ccagctccag gaacaggctc
      661 tcgagcgacc cgtcagcccc gtggctcgac ttgagctcgg ccagggtacc gacgaacacg
      721 atcctgccct tgttgatgat ggcgagcctg tcgcacaggc gctcggccac ctccatcacg
      781 tgcgtcgaga agaagaccgt cttgccagcg ctcgccctgg cccgcatgac ctccttgagg
      841 gcgaaggccg ccttcgggtc aaggccgacc atcggctcgt cgagcaccca gttcggcggg
      901 tcgggcagga ggctcgccgt cacgatgagc ttctgcttca tgccgtggga gaagctaccg
      961 atgctgcctc cgagcgcgtc gcgaatgccg aacaggccgg cgtactccgc caccctggcc
     1021 cggcgcgtct cggaatcgac gccgtacacg tcgccgacga agttgaggta ctcggcggcc
     1081 ttgagcctgg cgaacagctc cggattgtcg gtcacgtagc cgatgcggcg tttcgcctcg
     1141 agcgggtcga ccgctatgtc gactccgtcg accgaggcgc ggcccgagtc ggggcgcagc
     1201 acgccggtca gcaccttgat cgtcgtcgtc ttgcccgctc cgttcgggcc caggaagccg
     1261 aagatctcgc cgtcctcgat ggacagcgac aggtcgtcca ccgccttgac ggcgctcttg
     1321 gcgtaggtct tagaaatgcc cttcagttct atcatgctgt ctctccccac cggccgctac
     1381 tcgtcgcggc catcgtccca atccgtcgca tcgcccttac ggcggcggcg cacgctctcg
     1441 gtcagcagga actcgatctg cccattgatg gacctgaagt cgtcctcggc ccaggcggcg
     1501 agctcccgcc acaacgacgg cgacagccgc aagagtatct gctttttatc cttttccttg
     1561 tcgccgctca tgagccccta cgcctaatag atcgagccag aattcacgat cggcgacacg
     1621 tccttgttcg agcagagcac gacgagcagg ttgctgacca tcgcggcctt ccgctcctcg
     1681 tccaagtcga cgatgttgtt cgccttgagc ttgtccagtg ccagttccac catgccgacc
     1741 gcgccgtcga cgatcatttg tctggcggct atgaccgcgc tggcctgctg gcgctgcagc
     1801 atcgcggcgg cgatctccgg cgcgtacgac aggtgcgtga tgcgcgcctc gaggatctcg
     1861 atgccggccg cctcggcctt ggcctggatc tcggccctga gcttctcggc tatctcctgg
     1921 ctgctgcccc ggagcgaggt ctcgccctcg gagtcgctgg aatcgtaggg gtacaggcgc
     1981 acgatgttcc gcagcgcgg
//
LOCUS       DAOOLN010000289         1998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_71426_length_1998_cov_2.819866, whole genome shotgun sequence.
ACCESSION   DAOOLN010000289 DAOOLN010000000
VERSION     DAOOLN010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71426_length_1998_cov_2.819866"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(368..1597)
                     /gene="secF"
                     /locus_tag="PLW80_04520"
     CDS             complement(368..1597)
                     /gene="secF"
                     /locus_tag="PLW80_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPB65801.1"
                     /translation="MKRVINFHKAFLPATLASVVVLVAGLISLATMGLNLGVDFQAGI
                     NQSVRLAYPVADVSYEGPGSPVPSIGAGELSLVFSGADVDSRTVSWNLRTVGTIADLA
                     GLLKAEGVTMAVRDGAGLSADLLVPTYQGDYTLGASPRLVHRAPKSSAEAYGSIEEIR
                     AAVSSLGSVSVQNVGGADSMQYLIRVSDDGSDKSFSETIPGRIRAALESAFGADKVVV
                     IKTDYVGARFSEDLSSSAWKLSLFTALAILAYATIRFKMQYAVGAVLAILHDCLIMIA
                     FMVWTRMEFNTTSIAALLTILGYSINDTIVIFDRVREDRKLDPNAKMSAVIDRSVTET
                     LGRTVITTVTTLLAVAALYFFTSGSIKDFALALIVGIVSGTYSTIFIATGFVHLWDKY
                     GGKRQAKAGKLAAKPAN"
     gene            complement(1594..>1998)
                     /locus_tag="PLW80_04525"
     CDS             complement(1594..>1998)
                     /locus_tag="PLW80_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPB65802.1"
                     /translation="NLFLMFSILSAFNLTLTLPSIAGFVLTIGMAVDAKVIIFERIKE
                     ELRDGKGRKAAIDAGFAKAFWAVMDGNITTIIAALFLSQLGTGSIQGFAVSLAIGNIS
                     SLFTALFVSRLLFDFNTDVAKHRTVSIGWRVK"
BASE COUNT          390 a          578 c          677 g          353 t
ORIGIN      
        1 gaatcgaacc gccgcataaa ggttttgcag acctctccct taccgcttgg gtacggcgcc
       61 gtatttgtag ctgaatgtca aaagatccag tgcgccgtct agaaccggtg ccggatgaat
      121 atacctaaaa cgttcgatcg tgtaaagcgg tcgttggttg ctcgccgagg ccggccatga
      181 aggagaccgt cgtgcccacg ccgttcctgg aaccgatcat ctccgctgct gacatacagc
      241 ttcttcgtcc atcgttgcag gcggccgcga acgacccctc gcataggaaa cgcacccggc
      301 cttgcacgaa aagggccgaa ccgcgtgcgg ttcggcccga tggctttgag atatgcgacg
      361 aacggactca gttcgccggc ttggccgcca gctttccggc cttggcctgc ctcttgccgc
      421 cgtacttgtc ccagagatgc acgaacccgg tagcgatgaa gatggtcgaa taggtacccg
      481 agacgatgcc gacgatcagc gccagggcga agtccttgat actgcccgag gtgaagaaat
      541 agagcgctgc gacggccagc agggtcgtga cggtcgtgat gaccgtgcgg ccgagggtct
      601 cggtcacgga tcgatcgatg accgccgaca tcttggcgtt cgggtcgagt ttgcggtctt
      661 cgcggacccg gtcgaagatg acgatcgtgt cgttgatcga gtagccgagt atcgtgagga
      721 gcgcggcgat cgaggtggtg ttgaactcca tgcgggtcca gaccatgaag gctatcataa
      781 tgagacagtc gtgcaggatg gcgagcacgg caccgacggc gtactgcatc ttgaacctga
      841 tcgtggcgta cgcgagtatg gcgagcgcgg tgaacaggct gagcttccag gcgctcgaag
      901 acaggtcctc ggagaagcgc gcgccgacgt agtcggtctt gatgacgacg accttgtcgg
      961 cgccgaaggc cgactcgagg gcggcgcgta tcctgccggg gatagtctcg ctgaaggact
     1021 tgtccgagcc gtcgtcgctg acgcggatga ggtactgcat cgagtcggcg ccgccgacgt
     1081 tctgcaccga cacggagccg agcgacgaaa cggccgcgcg gatctcttcg atcgagccgt
     1141 aggcctcggc cgacgacttg ggcgcgcgat ggacgagccg cggagaggct ccgagggtat
     1201 agtcgccctg ataggtcggg acgagcaggt cggcggagag gcctgcgccg tcgcgtacgg
     1261 ccatggtcac gccctcagcc ttgagcaagc cggccaggtc ggcgatggtg ccgacggtgc
     1321 gcaggttcca ggatacggtc ctggagtcga cgtcggcccc ggaaaagacg agggatagct
     1381 cgccggctcc gatggacggc acgggcgagc cgggcccttc gtagctgacg tcggcgacgg
     1441 gatacgcgag ccgcacggac tggttgatgc ccgcttggaa gtccacgccc aggttgaggc
     1501 ccatcgtcgc cagcgagatg agtccggcga cgagcacgac gaccgaggct agggtcgcgg
     1561 gcaggaacgc cttatggaag ttgatgacgc gcttcatttg accctccagc caatcgacac
     1621 ggtgcggtgc ttagccacgt cggtgttgaa gtcgaataac agcctggaca cgaacagcgc
     1681 ggtgaacagc gacgaaatgt tgccgatcgc gaggctgacg gcgaagccct ggatggagcc
     1741 ggtgccgagc tgggacagga acagggcggc gatgatcgtc gttatgttgc cgtccatgac
     1801 ggcccagaag gctttcgcga agcccgcgtc gatggcggcc ttacggccct tgccgtcgcg
     1861 tagctcctcc ttgatgcgct cgaagatgat cactttcgcg tcgaccgcca tgcctatcgt
     1921 cagcacgaaa ccggctatcg acggcagggt cagcgtcagg ttgaaggccg acaggatcga
     1981 gaacatcagg aacaggtt
//
LOCUS       DAOOLN010000290         1998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_71436_length_1998_cov_2.382913, whole genome shotgun sequence.
ACCESSION   DAOOLN010000290 DAOOLN010000000
VERSION     DAOOLN010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71436_length_1998_cov_2.382913"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PLW80_04530"
     CDS             <1..420
                     /locus_tag="PLW80_04530"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPB65803.1"
                     /translation="SRFSEASMADYLPSLVREVVANYAHAADVRVETDAADVRLDVKR
                     LQALGILVNELVSNSMKHAFKGRASGLVFLRAGLEGGRVTVTVGDDGPGMPEPPPSDA
                     SGGGFGLTLVSLLATQLRADIARESDGGTVVRLSFEL"
     gene            complement(476..1249)
                     /locus_tag="PLW80_04535"
     CDS             complement(476..1249)
                     /locus_tag="PLW80_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPB65804.1"
                     /translation="MIHSARPGRPGLVVVGLLFMALTLVGVRAEAQTRVLMVTDTWPP
                     FRMEETSFGTIKDGIDFQIIRRIGDELGLELEIQYHPWARALAMLRSGQADIMTGVAY
                     SPERDEYLRYVPTAYAAVRPVFYTRTGMAASLRSYDDLAGKTIGQSASSVYFEPYDSD
                     ATLGKLSLQYEEQVLRMLALGRIDVAVGTEPNIGWDALRLGLSGQVEPALFRPDASTE
                     LFIVVSERSPLVARSGEIDAILRRMLASGEIDAIMAAYR"
     gene            1434..1943
                     /locus_tag="PLW80_04540"
     CDS             1434..1943
                     /locus_tag="PLW80_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rab family GTPase"
                     /protein_id="HPB65805.1"
                     /translation="MIKKKLCLLGSLGVGKTSLIRKFVLNVYSETYLCTVGVKVDKKI
                     VRLREGEDVILMIWDMEGQDDFSSLADTYLRGLSGYLCVADGTRPETLRTALSICSSM
                     NSMFQGVSSSLLVNKCDLTDRWAVTEEECHDLAPGCGRILRTSAKLGIGVEEAFVDIA
                     GRMVERPHG"
BASE COUNT          350 a          611 c          672 g          365 t
ORIGIN      
        1 tcgcgcttct cggaggcgtc catggccgac tacctgccct cgctcgtccg cgaggtggtc
       61 gccaactacg cccatgccgc ggacgtgcgc gtggagaccg acgcggcgga cgtgcgcctg
      121 gacgtgaagc ggctccaggc cctcggcatc ctggtcaacg agctcgtgtc gaacagcatg
      181 aagcacgcct ttaaggggcg agcgagcggg ctcgtgttcc tgcgcgccgg gctcgagggc
      241 ggccgggtga cggtgacggt gggcgacgac gggccaggta tgcccgagcc gccgccgagc
      301 gacgcgtccg gcggcggttt cgggttgacc ctcgtgtcct tactggctac gcaactccga
      361 gccgatattg ctcgcgagtc ggacggcggc acggtcgtgc gcctgtcttt cgagctgtaa
      421 tcggctacgc ccgcgtcctg gaggcacgcg ctggatatcg atccggagga ggcgtttatc
      481 gataggcggc catgatcgcg tctatctcgc cgctggccag catgcggcgg aggatcgcat
      541 cgatctcgcc ggatctcgcg acgaggggcg agcgctccga cacgacgatg aacaattccg
      601 tactggcatc tggtctgaag agggccggct cgacctggcc cgagagtccg agccggagcg
      661 catcccagcc tatgttcggc tcggtgccta cggcgacgtc gatgcggcct agggcgagca
      721 tgcgcaatac ctgttcctcg tattgcaggc tcaatttgcc gagggtagcg tcggagtcat
      781 acggctcgaa atatacggag ctagcgctct ggcctatcgt cttaccggcc aggtcgtcgt
      841 acgaccgtag cgacgcggcc atgcccgtcc gcgtgtagaa gacaggccgg accgcggcat
      901 aggccgtcgg cacgtagcgc aggtactcgt cgcgctccgg cgagtaggcc acgccggtca
      961 tgatgtccgc ctggccggac cttagcatcg cgagcgcccg cgcccaggga tgatactgga
     1021 tctcgagctc aaggccgagt tcatcgccga tccgcctgat gatctggaag tctatcccgt
     1081 ccttaatcgt accgaagctt gtttcttcca tccggaacgg cggccaagtg tcggtgacca
     1141 tgaggacgcg cgtctgggcc tccgcccgaa cgccgacgag cgtgagcgcc atgaacagga
     1201 gaccgacgac cacgaggccg gggcggcccg gtcgcgctga gtggatcatg tatctagtct
     1261 aggtttcccc gacaccgtag tcaaatgtag gttagtaggc ggctttgggg cgcctacccg
     1321 gttaggaccg ggatgcggat cagggtttgg cgatgacggc agcctccttg ggatagcttc
     1381 ctttacgctt cgcgtattct aggcgatact ggtacccgga gagtagaaaa cggatgataa
     1441 agaagaagct atgccttcta ggctcgctcg gcgtcggcaa gacgagcctg atacgaaaat
     1501 tcgtcctgaa cgtgtattcc gaaacctacc tgtgcactgt cggcgtcaag gtcgacaaga
     1561 agatcgtgcg cctgcgtgaa ggcgaggacg tgatcctgat gatctgggac atggaagggc
     1621 aggacgattt cagctctctg gccgacacct acctacgcgg cctgtccggc tatctgtgcg
     1681 tggccgacgg cacgcggccg gagacgctca ggacggccct gtccatctgt tcgtcgatga
     1741 actcgatgtt ccaaggcgtc agctcatcgc tactcgtcaa caagtgcgac ctgacggatc
     1801 gctgggcggt gaccgaggag gagtgccatg acctggctcc cggctgcggc cggatcctaa
     1861 ggacgagcgc caagctcggt atcggcgtcg aggaagcctt cgtcgacatc gccggccgca
     1921 tggtcgagcg cccgcatggc tgatcgccgg gccggtcggc ccatcgacga gcgcctgagg
     1981 gagctggacc cgcgggag
//
LOCUS       DAOOLN010000291         1998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_71437_length_1998_cov_2.361812, whole genome shotgun sequence.
ACCESSION   DAOOLN010000291 DAOOLN010000000
VERSION     DAOOLN010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71437_length_1998_cov_2.361812"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /gene="rplD"
                     /locus_tag="PLW80_04545"
     CDS             <1..437
                     /gene="rplD"
                     /locus_tag="PLW80_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPB65806.1"
                     /translation="GTGRARQGDVKSPIYVGGGIVFGPHPRDYSYSINKKAKRLAMKS
                     ILSLKAQGSTMKIVEDFSVDSGKTKDLASILAGLVPEGKAERTVVILKDDDSMVKRAG
                     RNIPWLHFLSYNRLRAHDLFYGRTVIVMESAVKSLNEFYADK"
     gene            452..736
                     /locus_tag="PLW80_04550"
     CDS             452..736
                     /locus_tag="PLW80_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPB65807.1"
                     /translation="MQYESILIEPVLSEKTNLLREEGQYVFRVDPRATKTQVKEAVKR
                     LFEVHPISCTIMNVKGKPKRLRYRSGYTSSWKKAVVRLAKGETIKAFEGA"
     gene            753..1580
                     /gene="rplB"
                     /locus_tag="PLW80_04555"
     CDS             753..1580
                     /gene="rplB"
                     /locus_tag="PLW80_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPB65808.1"
                     /translation="MAIKTFRPITPGLRHRVQVVNDALTVGNEPVKGLTKGKKQHAGR
                     ASNGRISMRRKGGGHKRKLHEIDWKRDKIGVPGKVATIEYDPNRSANIALINYVDGEK
                     RYIVAPAGLSVGQTVLSGPDAAPEVGNALPLENIPVGFTVHNVELTLGKGAQLARSAG
                     AGALIVGTDGDYVTLKLPSNELRLVFKKCIATIGGVGNEDHMNERFGKAGRVRWLGKR
                     PRVRGIAMNPVDHPHGGGEGRGKGYKQPVTPWGQPCKGYKTRDPHKPSSRFIVKRRK"
     gene            1597..1878
                     /gene="rpsS"
                     /locus_tag="PLW80_04560"
     CDS             1597..1878
                     /gene="rpsS"
                     /locus_tag="PLW80_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPB65809.1"
                     /translation="MSRSVKKGPFIEKSLYKKIIEASKAGDKKVLKTFSRCSTIIPEM
                     VGLTLSVYNGKSWVPVYVTENLVGHKLGEFSPTRVFRGHAGSDKKADRR"
     gene            1888..>1998
                     /locus_tag="PLW80_04565"
     CDS             1888..>1998
                     /locus_tag="PLW80_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPB65810.1"
                     /translation="MAKITGYPATAKYLMGSPSKIRPVADLVRNKPYNEAV"
BASE COUNT          444 a          609 c          628 g          317 t
ORIGIN      
        1 agggcaccgg ccgcgcccgc cagggcgacg tcaagtcgcc gatctacgta ggcggcggta
       61 tcgtcttcgg cccccacccg agggactact cgtactcgat caacaagaag gccaagcgcc
      121 tcgcgatgaa gagcatcctg agcctcaagg cgcaggggtc gacgatgaag atcgtcgagg
      181 acttctcggt cgacagcggc aagaccaagg acctcgcgtc cattctggcc ggtctcgtcc
      241 ccgaaggcaa ggctgagagg accgtcgtga tcctgaagga cgacgactcg atggttaagc
      301 gcgcgggtcg caatatcccg tggctacact tcctgtcgta taaccgcctc cgcgcccacg
      361 acctgttcta cggacggacc gtgatcgtga tggaatccgc ggtgaagagc ctcaatgaat
      421 tctacgcgga caaataagga aggctgaagc aatgcagtac gaaagcatac tgatagagcc
      481 tgtcctcagc gagaagacga accttctgcg cgaagaggga cagtacgttt tccgcgttga
      541 cccgagggcg accaagaccc aggtcaagga agccgtaaag cggctcttcg aggtgcaccc
      601 gatctcgtgc accatcatga acgtcaaggg caagccgaag aggcttcgct accgctccgg
      661 atacacctcg tcgtggaaga aagccgtcgt ccgcctcgcc aagggcgaga ccatcaaggc
      721 tttcgaaggc gcgtgaggaa taggggaacg ctatggctat taagacattt aggccgataa
      781 cccccggact caggcaccgg gtgcaggtcg tcaacgacgc gcttaccgtc ggcaacgagc
      841 ctgtcaaggg cctcaccaag ggcaagaagc agcacgccgg ccgcgcctcg aacggccgca
      901 tatcgatgcg gcgcaagggt ggtggtcata agaggaagct ccacgagatc gactggaagc
      961 gcgacaagat cggtgtgccc ggcaaggtcg cgaccatcga gtacgatccg aatcggtccg
     1021 ccaacatcgc cctgatcaat tacgtcgacg gcgagaagcg ctacatcgtc gcccccgccg
     1081 gcctgtcggt cggccagacg gtgctgagcg gccccgacgc cgcccccgag gtcgggaacg
     1141 cgttgccgct cgagaatatc ccggtcggct ttaccgttca caacgttgag ctgaccctgg
     1201 gcaagggcgc tcagctcgcc cgctccgccg gggctggagc cctgatcgtc ggtacggacg
     1261 gcgattacgt aacgctgaag ttgccgtcga acgagctccg gctcgtgttc aagaagtgta
     1321 tcgccacgat cggcggcgtc ggcaacgaag accatatgaa cgagcgtttc ggcaaggccg
     1381 gccgcgtccg atggctcggt aagcggccgc gggtgcgcgg tatcgccatg aaccccgtcg
     1441 accacccgca cggtggcggc gagggccgcg gcaagggcta caagcagccg gtcaccccgt
     1501 ggggccagcc ttgtaagggt tataagacgc gcgatccgca caagccgtcg agccgcttca
     1561 tcgtgaagcg ccgcaagtaa aggataggag cgaaccgtgt ccaggtcagt aaagaagggc
     1621 ccgttcattg agaagagcct ctacaagaag ataatcgagg cgtcgaaggc gggcgacaag
     1681 aaggtcctca agaccttctc ccgctgctcc acgatcatcc ccgagatggt cggcctcacc
     1741 ttgtcggtct ataacggcaa gagctgggta ccggtctatg tgaccgagaa cctcgtggga
     1801 cataagctcg gcgaattctc gccgacccgc gtgttccgcg gccacgccgg ctccgacaag
     1861 aaagcggaca ggagataggt ggctagcatg gcaaagatta ccggttaccc ggcgaccgcc
     1921 aagtacctga tggggtcgcc gtcgaagatt cggcccgtag cggaccttgt ccgcaacaag
     1981 ccgtataacg aggcggtc
//
LOCUS       DAOOLN010000292         1997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_71466_length_1997_cov_2.561277, whole genome shotgun sequence.
ACCESSION   DAOOLN010000292 DAOOLN010000000
VERSION     DAOOLN010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71466_length_1997_cov_2.561277"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PLW80_04570"
     CDS             <1..539
                     /locus_tag="PLW80_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65811.1"
                     /translation="GAAGPLAAPDAAGRPDAPGRPDAPGRPGVGDVTLGPGAALLIEG
                     SAAVSGLPGFGRNAFKGLRGVYAYRPDAGEARYALRVWYTSEPLLLGDGWAAVACPRL
                     PSPWRALAAAAPEVAPEGSSFWALSSGGTTLLMEFPEDMPEPCRFAAALVDRFAFFSR
                     YAQGPGDLSFPATLEVGR"
     gene            complement(536..1108)
                     /locus_tag="PLW80_04575"
     CDS             complement(536..1108)
                     /locus_tag="PLW80_04575"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPB65812.1"
                     /translation="MTPGDDGGTGYTLIRPGLEQLRTLYDWKAAESRRERFTCRPVKP
                     LGPFDEYAAKLSSWLREPMNLLFALVASGSPEPLGEVKGFDWNERNHSLEFGYYLPPG
                     NRGKGYGRILARLFVERVFGDGEAGVNKLYATTSAENAPSIRTLEGLGFRLDGRNREH
                     YWMDGERYDQCVYSLLASEWLASGDERRGP"
     gene            complement(1105..>1997)
                     /locus_tag="PLW80_04580"
     CDS             complement(1105..>1997)
                     /locus_tag="PLW80_04580"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPB65813.1"
                     /translation="GGVRGAASIAAVLRERGVRASFLLDEGGFVAEGMLGFADRPLAL
                     VGVAEKGYVDVAIVASGSAGHASMPPRHTAAGLVAKAVSLSEARPFPARITATLARFL
                     GDLAPYAPFGYRLLFRNLLVTAPLVKAAFSAAPTTNALVRTTCAATMLSGSDKENVLP
                     DRASAVLNVRVLPGDDVAGTMARLDAIAARAGARADFAHHGYAVEPLPESPVDHDGYR
                     LLKAAIGEVFPEAGAVPFMFTAGTDTKHYVGLCEAIYRFEPILQSADDLKGVHGPNER
                     VSVDNARRCCLFYETLLRSL"
BASE COUNT          277 a          680 c          735 g          305 t
ORIGIN      
        1 cgggcgccgc cggcccgctg gcggctcccg acgctgccgg gcggccagat gctcccgggc
       61 ggccagatgc tcccgggcgg ccaggcgtcg gcgacgtgac gctcgggccg ggcgcggccc
      121 tgctgatcga gggttccgcc gcggtgagcg ggctgcccgg cttcggccgg aacgcgttca
      181 agggcctgcg tggcgtctac gcctaccggc cggacgcggg cgaggcgcgg tacgcgctcc
      241 gcgtctggta tacctccgag ccgctcctct tgggcgacgg ctgggcggcg gtcgcatgcc
      301 ctcggctacc ctcgccctgg cgggcgctcg cggcggccgc gccggaagtc gcgcctgaag
      361 gctcatcgtt ctgggcgcta tcgtccggag ggacgacgct gctcatggag ttccccgagg
      421 atatgcccga gccctgccgc ttcgcggcgg ccctcgtcga ccgtttcgcc ttcttcagcc
      481 gctacgccca ggggccgggc gacctatcgt ttccggctac gctcgaggtc gggcgttagg
      541 ggcctcgccg ctcgtcgccg gaggccagcc actcgcttgc taggagcgaa tagacgcatt
      601 ggtcgtagcg ttcgccgtcc atccaatagt gttcgcgatt ccttccgtcg agcctgaagc
      661 cgagaccctc gagcgtacgg atggacggcg cgttctcggc cgaggtggtt gcgtagagct
      721 tgttgacgcc ggcctccccg tcgccgaaga cgcgctcgac gaagagacgc gccaggatcc
      781 tgccgtaacc cttgccccgg ttgcctggag gcaggtagta gccgaactcg aggctatggt
      841 tgcgctcgtt ccagtcgaag cccttcacct cgccgagcgg ttccggcgat cccgacgcga
      901 cgagcgcgaa gagcaggttc ataggctccc gtagccacga actcagcttg gcggcgtact
      961 cgtcgaaggg gccgagaggc ttgaccggcc tgcaggtgaa gcgctcgcgc cggctctcgg
     1021 cggccttcca gtcgtacagc gtacgtagct gttcgaggcc cggccgtatg agcgtatacc
     1081 cggtgccgcc gtcgtcgccc ggcgtcatag agacctcagg agcgtctcgt agaacaggca
     1141 acaacgccgc gcgttgtcga ccgagacgcg ctcgttcggg ccgtgcactc ccttgaggtc
     1201 gtcggccgac tggaggatgg gttcgaagcg gtagatcgcc tcgcatagcc cgacgtagtg
     1261 cttggtgtcg gtgcccgcag tgaacatgaa cggcaccgcg ccggcctccg ggaagacctc
     1321 gccgatggcc gccttcagta agcgatagcc gtcgtggtct accggcgact cgggcagggg
     1381 ctcgacggcg tagccgtgat gggcgaagtc ggccctggcg ccggccctgg cggcgatcgc
     1441 gtcgagcctg gccatcgtcc cggccacgtc gtcgccgggc aacacccgca cgttcagcac
     1501 cgcgctggcg cggtcgggca gcacgttctc cttgtcggag ccggacagca tcgtcgcggc
     1561 gcaggtcgtg cgcacgaggg cgttggtcgt cggcgcggca gagaaggcgg ctttgacgag
     1621 gggcgcggtg acgagcaggt tcctgaacag cagccgatag ccgaaggggg cgtagggcgc
     1681 caggtccccg aggaagcgcg ccagggtcgc ggtgatcctg gccgggaagg gtctggcctc
     1741 ggacagggag acggccttgg cgaccaggcc ggcggccgtg tgacgcggcg gcattgaggc
     1801 gtgtccggcc gagcccgagg cgacgatcgc gacgtcgaca tagcccttct cggcgacgcc
     1861 tacgagggcg agcgggcggt cggcgaagcc gagcatgccc tcggcgacga agccaccctc
     1921 gtcgagcagg aacgacgcgc ggacgccgcg ctcgcgcagg accgccgcga tggacgcggc
     1981 gccgcgcacg ccgccgg
//
LOCUS       DAOOLN010000293         1995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_71586_length_1995_cov_2.408247, whole genome shotgun sequence.
ACCESSION   DAOOLN010000293 DAOOLN010000000
VERSION     DAOOLN010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71586_length_1995_cov_2.408247"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PLW80_04585"
     CDS             complement(<1..639)
                     /locus_tag="PLW80_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB65814.1"
                     /translation="MSMVRSWTLGRVISLVVLLIATVFSVFPFVWMLVGATLNPSEVL
                     RGALRPGAEFFTNWVKATRMYNLPLFFWNSLKIAVVTVLLGLLVNGFAAYGFEKYPSK
                     ARDRVFNVLLLTLIIPQIALVIPMFRMFAFLRLLDSHAAIILPAIMSVFIIFFMRQNF
                     KMFPSEIIEAARADGASELTIFAGIVTPSMQATYVSSAIYIFINQWNSYLWPL"
     gene            complement(636..1529)
                     /locus_tag="PLW80_04590"
     CDS             complement(636..1529)
                     /locus_tag="PLW80_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB65815.1"
                     /translation="MKQKASFQRYLNRFGWFCVLPSVILFGVFMLYPILSSLYYVTRR
                     WKGMSNTFIGLGNFIRMAQDKVFLSALGNNFLFMVVQIPLMVFLALVLAVILNQGIRR
                     WKGAFRLAIFLPAVTSLVVVSVLFRVLLQENGMLNNVLLKAHLITQPIGWLNDAAWAK
                     VTIILAMTWRWTGYNMVFFLVGLQSIDPQIYEAAEVDGASKTRQFFTITIPLLIPVIL
                     FSVVTSTSGTMQLFDEPNILTWQGGPANATMTTALYIYRQAFFINADFGYATAMSYVV
                     VLISGIFAFAQMRLLGDRGNS"
     gene            complement(1617..>1995)
                     /locus_tag="PLW80_04595"
     CDS             complement(1617..>1995)
                     /locus_tag="PLW80_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65816.1"
                     /translation="YVFSSSKNKARAVDLLKSVWATATPDNLEFYNKILKDAGAMGTF
                     LPSQSGSNYKAGDDFFYRGQALSKDFASWMANVPVLKYTPNYVPMRDAVAHATLNYFQ
                     GKIATIDEAIAAAETEYKQVTGK"
BASE COUNT          395 a          549 c          687 g          364 t
ORIGIN      
        1 gagcggccag aggtacgagt tccactgatt gatgaagatg tagatggccg acgatacgta
       61 ggtcgcctgc atcgacggcg tgacgatgcc ggcgaagatg gtcagctcgc tcgccccgtc
      121 ggcccgcgcc gcctcgatga tctcggacgg gaacatcttg aagttctggc gcatgaagaa
      181 gatgatgaac accgacatga tggccggcag gatgatggcg gcgtgcgagt cgagcagccg
      241 gaggaaggcg aacatcctga acatcgggat gacgagggct atctgcggga tgatgagcgt
      301 caggagcagc acgttgaaga cccggtcgcg cgccttggac gggtatttct cgaagccgta
      361 ggccgcgaat ccgttgacga gtaggccgag tagcacggtg acgacggcta tcttgagcga
      421 gttccagaag aacagcggca ggttgtacat gcgcgtggcc ttgacccagt tcgtgaagaa
      481 ctcggctccc ggccgcagcg cgccgcgcag cacctcactc gggttgagcg tggccccgac
      541 gagcatccat acgaagggga agacgctgaa gacggtggcg atgagcagca cgacgaggct
      601 gatgacccgt ccgagcgtcc aggagcgtac catgctcatg agttccccct gtcgccgagc
      661 aggcgcatct gcgcgaaggc gaatatgccc gagatgagca cgacgacgta cgacatcgcg
      721 gtggcgtagc cgaaatccgc gttgatgaag aaggcctgcc ggtagatgta cagggccgtg
      781 gtcatcgtcg cgttggccgg ccctccctgc caggtcagga tattcggctc gtcgaagagc
      841 tgcatcgtgc cgctcgtcga ggtgacgacg gagaacagga tcaccggtat gaggagcggt
      901 atcgttatcg tgaagaactg cctcgttttc gacgctccgt cgacctctgc ggcctcgtag
      961 atctgcgggt cgatgctctg gagcccgacg aggaagaaga ccatgttgta gccggtccag
     1021 cgccaggtca tcgcgaggat gatggtgacc ttggcccagg ccgcgtcgtt gagccagcct
     1081 atcggctggg ttatcaggtg cgccttgaga agcacgttgt tgagcatgcc gttctcctgg
     1141 aggagtaccc tgaacagcac cgacacgacg accagcgagg tgacggccgg caggaagatg
     1201 gccagcctga aggcgccctt ccagcgccgg atgccctggt tgagtatcac ggccagtacg
     1261 agcgccagga acaccatcag cggtatctgt acgaccatga acaggaagtt gttgccgagg
     1321 gcggacagga agaccttatc ctgggccatg cggataaagt tgccgagccc gatgaaggtg
     1381 ttgctcatgc ccttccagcg gcgcgtcacg tagtacagcg acgacaggat ggggtagagc
     1441 atgaagacgc cgaagagtat gaccgacggc aggacgcaga accagccgaa gcggttcagg
     1501 taacgttgaa agctggcctt ctgtttcatg ggggctcctg gttgcctgga ctgggggaac
     1561 gcgggggagc cgcgccggcg gagggcccgt cggcgcggct ccttatcggg cgtccgttat
     1621 ttgccggtca cctgcttgta ctcggtctcg gccgccgcta tggcctcgtc gattgtcgcg
     1681 atcttgccct ggaagtagtt gagcgtggcg tgggccacgg cgtcgcgcat cggcacgtag
     1741 ttcggcgtgt acttgagaac cggcacgtta gccatccacg aggcgaagtc cttggagagc
     1801 gcctggcctc tatagaagaa atcgtcgccg gccttgtagt tggagccgga ctggctcggc
     1861 aggaaggtgc ccatggcgcc ggcgtccttg agtatcttat tgtagaactc gaggttgtcc
     1921 ggggtggcgg tcgcccagac ggacttgagc aggtcgaccg cgcgggcctt gttcttgctg
     1981 gacgagaaga cgtac
//
LOCUS       DAOOLN010000294         1991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_71779_length_1991_cov_2.907025, whole genome shotgun sequence.
ACCESSION   DAOOLN010000294 DAOOLN010000000
VERSION     DAOOLN010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71779_length_1991_cov_2.907025"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..646)
                     /locus_tag="PLW80_04600"
     CDS             complement(239..646)
                     /locus_tag="PLW80_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB65817.1"
                     /translation="MKSEAILCVDDEAIILMAMKRELKTHFRDRFRFETALDAAEGLR
                     IIDALSEDGVSVILVISDWLMPGMKGDEFLLRIRSLHPDIRTIMVTGHADPASIERTI
                     KEAGAFTVLQKPWNRGELIAAISACVGPERSLA"
     gene            complement(643..1770)
                     /locus_tag="PLW80_04605"
     CDS             complement(643..1770)
                     /locus_tag="PLW80_04605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB65818.1"
                     /translation="MSGRSDLPGDFGDTYSKLLGLDESFSVKSHYPEFKAAERALLDI
                     NENLERALAELRNTQERLVQTRYQASVGRVLAAIAHELNSPLASTQSAADHLASIRRE
                     AIVAYRAMPAPLAGLVDRLLERQDDPPLVGRERREALAKRLAEAGCIGADDLADDLCD
                     LGRVDVTEDDVALLSGPRGPEAVNVAWVLGSMFGSSSLVARAARRSAGVVRSLNAYLE
                     LSPPDESALVDFDIAEKLLGRYCEAGSTGRTVRFELEDGVSAYGDPDRLDLVWENLIS
                     NALFAAGIDGNVTVRARFDGAATVVEVEDDRPGVDPAIRDRLFEPYVSAKALEEGKGL
                     GLDICKRIVDTHRGTITFESRPGRTVFTVRLPGEPDGADSL"
     gene            complement(1754..>1991)
                     /locus_tag="PLW80_04610"
     CDS             complement(1754..>1991)
                     /locus_tag="PLW80_04610"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPB65819.1"
                     /translation="DLGASPGDIASGGVKARLLELVRGLRARVGIAAGLSGIGVSRAD
                     LSRLAGFASMDACLATNPVSLSADDIERIYERAL"
BASE COUNT          341 a          664 c          643 g          343 t
ORIGIN      
        1 cgtcgcccat ggcggcgtcg ggcggcgcct cggccgcgac ggcctcggag agggccgggt
       61 cggcgccttt ctctacgagc atatcggcta cggtcgccgc caccgcggct ttccacgctt
      121 ctcgtacgga tctcacggct ggctcctgct gggcgatacc cgactcgagg ccgggccgcc
      181 atggtatgga cgccagtgta acgaataggg cggctcgttt caacgagtcg agccggggct
      241 aggccaagct tcgctccggc ccgacgcagg cggagatcgc cgctatgagc tcgcccctgt
      301 tccagggctt ctggagcacc gtaaaggccc cggcctcctt gatcgtccgc tcgatggagg
      361 cgggatcggc gtggccagtg accatgatgg tccgtatatc gggatggagg gatcgtatcc
      421 tcaggaggaa ttcgtcgccc ttcatgccgg gcatgagcca atcggaaatg acgagtatga
      481 ccgagacccc gtcctcggac agggcatcga tgatcctgag cccttcggcc gcgtccaaag
      541 cggtctcgaa ccggaaccga tccctaaaat gcgtcttgag ctcgcgcttc atggccatca
      601 ggatgatggc ctcgtcgtcg acgcacagga tcgcttcgct cttcatagac tgtccgctcc
      661 gtcgggttcg cctggcaggc gcaccgtgaa gacggtacgt ccaggacggc tctcgaatgt
      721 gatcgtaccc ctatgagtat ccactattcg cttgcatatg tccaagccca agcccttgcc
      781 ctcctcgagc gccttggccg atacgtatgg ctcgaacagc cgatcgcgga tggccggatc
      841 gacgcccggg cggtcgtcct ccacctccac gacggtcgcc gctccgtcga aacgcgccct
      901 gaccgtgaca ttcccgtcta tcccggccgc gaacagcgcg ttcgatatga gattctccca
      961 aaccaggtcg agcctatcgg ggtcgccgta ggcgctcacg ccatcctcga gctcgaagcg
     1021 aacggttcgg cccgtggacc cggcctcgca gtagcggccg agaagcttct ccgctatatc
     1081 gaaatcgacg agggccgact cgtccggcgg ggacagctcc agataggcgt tcagcgatcg
     1141 tacgactccg gctgaacgcc tcgccgccct cgcgacgagc gacgatgagc cgaacatcga
     1201 tccgagcacc cacgcgacgt tgacagcctc ggggccgcgg ggcccggaca ggagcgccac
     1261 gtcgtcttca gttacgtcga cccgccccag gtcgcacagg tcgtcggcca agtcgtcggc
     1321 cccgatgcaa ccggcctcgg ctagccgctt ggcgagcgct tcgcgccgct ccctgccgac
     1381 aaggggcgga tcgtcctgcc gttcgaggag ccgatcgacc agcccggcca ggggagccgg
     1441 catcgctcgg taggcgacga tggcttcccg cctgatcgag gcaaggtgat cggcggccga
     1501 ttgggtggac gcgagcgggc tattgagctc gtgggctatc gccgcgagga cacgaccgac
     1561 cgaggcctgg tacctcgtct ggacgagacg ctcctgggta ttacgcaact cggccagggc
     1621 tcgctcgagg ttctcgttta tatcgagtag ggcccgctcc gcggccttga attcagggta
     1681 atggctttta acggagaacg actcgtcgag accgaggagc ttgctgtagg tgtccccgaa
     1741 gtcgccgggc aggtcagagc gcccgctcat agatcctttc gatatcgtcg gcggataacg
     1801 acacggggtt ggtggccaga caggcgtcca tggaggcgaa accggccagc cgggacaggt
     1861 cggcccgcga cactccgatg cccgagaggc cggcggctat gccaacccgg gccctaagcc
     1921 cgcgaaccag ctcgagtaga cgggccttga cgcctccgga cgctatatcg ccgggggacg
     1981 cgcccaggtc g
//
LOCUS       DAOOLN010000295         1991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_71780_length_1991_cov_2.839876, whole genome shotgun sequence.
ACCESSION   DAOOLN010000295 DAOOLN010000000
VERSION     DAOOLN010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71780_length_1991_cov_2.839876"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PLW80_04615"
     CDS             <1..526
                     /locus_tag="PLW80_04615"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPB65820.1"
                     /translation="CVQYALRTARLELRLAAQFRARSTVKTYLAIVRRGPSADSGRLD
                     GWLARDPRERKRFAPSSEGVGKRALTEWTVLARAGGYAVLALGLRTGRTHQLRVHCKA
                     LGCPILGDPIYGSPDGRFPGASLMLHAWELRIRLPGDDEPSTFRAPVPERFAATAAAL
                     GLDGFPLPPGGAAL"
     gene            complement(523..795)
                     /locus_tag="PLW80_04620"
     CDS             complement(523..795)
                     /locus_tag="PLW80_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPB65821.1"
                     /translation="MSKKIYVGNMNYATTEDSLRELFGQFGTVESVAVVVDRATGRAK
                     GFGFIEMESPEAAQAAIQKLDGQEFEGRKLRVNEAQEKPRDSGPRY"
     gene            1076..1991
                     /locus_tag="PLW80_04625"
                     /pseudo
     CDS             1076..1991
                     /locus_tag="PLW80_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945573.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
BASE COUNT          383 a          635 c          661 g          312 t
ORIGIN      
        1 gtgcgttcaa tatgccctgc gcacggcgcg cctcgagctt cgccttgccg cccagttccg
       61 cgccaggagc accgtcaaga cttacctggc gatagtcagg cgcggcccgt cggcagattc
      121 cggcaggctc gacggctggc tagcccgcga cccgcgcgag cgcaagcgct tcgcgccgtc
      181 gtccgagggc gtcggcaagc gcgcgcttac ggaatggacg gtcctcgcca gggcgggggg
      241 ctacgccgtc ttggcgctcg gcctgcggac cggcaggacg caccagctgc gcgtccattg
      301 caaggcgctc ggctgcccga tactcggcga cccgatctac ggctcgccag acggccgctt
      361 tcctggagcc tcgctgatgc tacacgcctg ggagctgagg atcaggctgc caggggacga
      421 cgagccgtcc acgttcaggg cgcccgtacc ggagcgcttc gccgcgaccg cggcggcgct
      481 cggcctcgat ggctttccgt tgccgccggg cggcgcggcg ctctagtagc ggggtcccga
      541 gtcccggggc ttctcctgcg cctcgttgac gcgcagcttg cggccctcga actcctgccc
      601 gtccagcttc tggatcgcgg cctgggcggc ctcggggctc tccatctcga tgaagccgaa
      661 gcccttggcg cggccggtgg ccctgtcgac gacgacggct accgactcga ccgtcccgaa
      721 ctgcccgaac agctcccgta ggctgtcttc cgtcgtagcg tagttcatgt ttccgacata
      781 gatcttcttg gacatcgcgc gaatctccat gagccggcga ctgagccggg ttaggctggc
      841 ccgagacagc tcgggcgggc gccccgcggc gccaatgcga tgatacagcg ggcccggccc
      901 ggcgtcaacg tggatagaaa cgcgccatgg aagcggttag gcgtcggcgc gcgtatgttt
      961 tcttgacaaa ggctcgccgt tcactataat gatggcgata ttctattaaa agctggagga
     1021 tacagtggct atactgtata tagcccttcc tcttgccggt cttttcctgg gatggatgat
     1081 ccgctggctg tacgccagat ttcaacttac ggccgccgag cagaaggcgg cgcgtataac
     1141 gcaagaagcg gtaaaacagg ccgaggcgca gaagagagaa atactgctcg aggccaaaga
     1201 tcagctcatc agggagagaa accagcaaga gcgggagact cgggaacgca gggccgagat
     1261 acaacgctac gaacggcgcc tagtacagaa ggaagagaac ctcgataaga aggtcgagtc
     1321 gctcgagaag caggaaaccg ctctcgacga acgcgataag gtcatggcgg agaaggagga
     1381 attcctttcc ggccaggagg agcgctaccg caaggaactc gaacgcatct cgggcctgag
     1441 cgccgaggaa gccaagcgcc tgatcattca ggggctcgag aacgaggcca ggcacgatgc
     1501 ccaggccctg ctcaacaaga tcgagcagga ggcccagacg gcggccgaga agcgcacgcg
     1561 cgacatcctc gtcaccacga tgcagcggct cgccgccgac gtcaccgccg aggtgaccgt
     1621 cacaagcgtg tccctgccgg gcgacgagat gaaggggcgc atcatcggac gcgagggccg
     1681 caacatacgc accctcgaga cgctgaccgg cgtcgacgtg atcatcgacg acacgccgga
     1741 ggccgtggtc atctcgtgct tcgacccggt gcgcagggag atagcccgcg tatcgctcga
     1801 gcggctcatc tccgacggcg cattcacccg gcccgtatcg aggaggtcgt ccagaaggtc
     1861 agccgcgaga tatcgcagaa gatcttcgag gagggcgaga aggtcgtctt cgacatgggc
     1921 ttgcacaacc tgaacccgga gctgatccgg gcgctcggtc tggagggccc cgcaagcgaa
     1981 gcgcgggagc a
//
LOCUS       DAOOLN010000296         1987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_72014_length_1987_cov_1.737060, whole genome shotgun sequence.
ACCESSION   DAOOLN010000296 DAOOLN010000000
VERSION     DAOOLN010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72014_length_1987_cov_1.737060"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            377..1372
                     /locus_tag="PLW80_04630"
     CDS             377..1372
                     /locus_tag="PLW80_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HPB65822.1"
                     /translation="MKHSRIITVIAIVAIAAVAFSSCAKKEEAAKPAAIDKNSVFITV
                     ASGPTSGLYYPIGGGFGSVVANDIGFRSSVQSTGASVENINLIMNKKAEIAIVMSDAV
                     LQAYDAFGAFEGQEPKKDLRGLMSLYPNYVQLVTLESTGIKSFADLKGKRVGIGAPNS
                     GVEVNARLMFEAHGMSYADIRPDYLNYGEAIDQMRNGLVDAAFVTSGIPNATVMDLGT
                     TNKIRIIPIEGEGMKTLQTKYPFFTSSVIPAGTYGNAADINTATIMNILIVQKDLSDE
                     VVYMMTKGFFENIGKIQDTHSAAKKHINMETALDGMVVPLHPGAERYYKEVGKLK"
     gene            1369..1881
                     /locus_tag="PLW80_04635"
     CDS             1369..1881
                     /locus_tag="PLW80_04635"
                     /inference="COORDINATES: protein motif:HMM:NF020479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HPB65823.1"
                     /translation="MTTIRGRRALAAAARAMVAAALAAAVGVVMSLASCAGRNGLRLV
                     VETQRDGRRLLSVPVSSGDRLEFEWIHSVERFPWYEYFELEADGSLFLRETRIGGFGA
                     GVPYDRGTSASVEDGFVVYRGIDERFPSYRWINSSTAVAAISINGTVVARGTDFPHHE
                     ALELRVTPRR"
BASE COUNT          402 a          662 c          609 g          314 t
ORIGIN      
        1 ggcctatctc ggcggcaaga gcctcgcgga gctcgaggcc atagcgatca aggccgccct
       61 cgaacgaaac ggcggccggc gtcgcgagac ggccacgagc ctaggcatca gcgagcgcgg
      121 gctacgcaac aagatccacg agtacggcct cggctgagtc gtcagagcgg cagcaaatgc
      181 cgagagcggc aaatactacc gttcgatatg agacagggag tcataaccag ctcccaccag
      241 ccgtcgccct aaagagtgcc acgctatacc agacatgcaa ttagataggc ttatgcgcga
      301 cagtccgaga cgcggcgtac catggcacga atcttgcaat gcgtttaggt agcactacgt
      361 tcccaaggag gttcccatga agcactcgag gatcatcacc gtaatcgcca tcgtagcgat
      421 cgccgccgtg gcgttctcgt cctgcgccaa gaaggaagag gcggccaagc ccgccgcgat
      481 cgacaagaac tccgtcttca tcaccgtcgc gtccggcccc accagcggcc tgtactaccc
      541 gatcggcggc ggcttcggtt ccgtcgtcgc caacgacatc ggcttccgct cgtcggtcca
      601 gtcgaccggc gcgtcggtcg agaacatcaa cctgatcatg aacaagaagg ccgagatcgc
      661 catcgtcatg tcggacgcgg tgctccaggc ctacgacgcc ttcggcgcct tcgagggcca
      721 ggaacccaag aaggacctgc gcggcctcat gagcctgtac ccgaactacg tccagctcgt
      781 caccctcgag tcgaccggca tcaagagctt cgccgacctc aagggcaagc gcgtcggcat
      841 cggcgcccct aactcgggcg tcgaggtgaa cgcgcgcctc atgttcgagg cccacggcat
      901 gagctacgcc gacatccgcc ccgactacct gaactacggc gaggcgatcg accagatgcg
      961 caacgggctc gtcgacgcgg ccttcgtcac gagcggcatc ccgaacgcca ccgtgatgga
     1021 cctcggtacg acgaacaaga tccgcatcat cccgatcgag ggcgagggca tgaagaccct
     1081 ccagacgaag taccccttct tcacctcgtc ggtcatcccc gccggcacct atggcaacgc
     1141 cgcggacatc aacaccgcca ccatcatgaa catcctgatc gtccagaagg acctatcgga
     1201 cgaggtggtg tacatgatga ccaagggctt cttcgagaac atcgggaaga tccaggacac
     1261 ccacagcgcc gccaagaagc acatcaacat ggagaccgct ctcgacggca tggtcgtccc
     1321 gctgcacccg ggcgccgagc gctactataa agaagtaggc aagctgaagt gacgacgatc
     1381 cgcggccggc gggccctggc cgccgcggcg cgggccatgg tcgccgcggc cctagccgcg
     1441 gcggtcggcg tcgtcatgtc gctcgcgtcg tgcgcagggc ggaacggcct gcgtctggtc
     1501 gtcgagaccc agcgggacgg gcggcgcctg ttgtccgtgc ccgtctcgtc gggagaccga
     1561 ctcgagttcg agtggataca ctcggtcgag cgcttcccat ggtacgagta tttcgagctc
     1621 gaggccgacg gctcgctgtt cctgcgcgaa acgcggatcg gcggcttcgg ggccggcgtg
     1681 ccctacgatc gcgggacgag tgcctcggtc gaggatggct tcgtcgtcta tcgaggcatc
     1741 gacgagcgct tcccgtccta ccgctggatc aattcttcaa ccgcggtggc cgccatctcg
     1801 atcaacggga cggtcgtcgc gcgcggcacc gattttccgc accacgaggc ccttgagctt
     1861 cgggtcacac cacggaggta acgccttgag cgaaacgata ggaacggcgg ccacgccgga
     1921 tatgcagaag aagattctcg aggagttcga ctccgagtcg cgctaccgcg agttcggcgg
     1981 caaggtc
//
LOCUS       DAOOLN010000297         1986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_72048_length_1986_cov_3.116002, whole genome shotgun sequence.
ACCESSION   DAOOLN010000297 DAOOLN010000000
VERSION     DAOOLN010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72048_length_1986_cov_3.116002"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PLW80_04640"
     CDS             complement(<1..516)
                     /locus_tag="PLW80_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HPB65824.1"
                     /translation="MQDSSSQGQAVENNGGAKVKQVIGVSSGKGGVGKSTVAVLLAEA
                     LAARGAKVGLLDADITGPSVPRLLGLSSFRGESDGSMLVPITSEDGIKVVSINFYVGD
                     ENTPVVWRGPLLSKAIEQFWTDVRWGELDYLIVDFPPGTGDVQITAFNKLPIDGVVVA
                     TTPQSLVSMIVA"
     gene            678..1883
                     /locus_tag="PLW80_04645"
     CDS             678..1883
                     /locus_tag="PLW80_04645"
                     /inference="COORDINATES: protein motif:HMM:NF019174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HPB65825.1"
                     /translation="MSYELTSEHEKILSSLLRFVTVAGVLAYLPGTVVGFLESLWAVV
                     AVNTIAYATVAAVAFIPGVGYRAKLTVLVGACLAVGSVVLVSTGPLGAGYIWLLVGVV
                     LSALFGRKRAVALTIGATMAIMIGWAVALGVGADGRGATPWNVGIIAASLLVICLALS
                     FIIRRLQGSLASAFEDKARLADSLAAELQESHSTKDRLESTLGEMDELLRELQHRVRN
                     NLQTVASLLALDERPPTSRAQGPDEPLERARRRIRALTVANDCFLDGGDGGRADAWGL
                     IRSAAQSADEDHRGCDCRLLRTDRGEPTIDPHAAPLVAILVSDVAASLGSLGRMALVV
                     KPGDGVVVEARCLAPGENPEARVVYRAIASGRVARSAEPEIRLSFIEPDDGSGPGIRL
                     EYREGRVST"
BASE COUNT          315 a          651 c          662 g          358 t
ORIGIN      
        1 ggccacgatc atcgacacca gcgactgggg ggtcgtcgcg acgaccacgc cgtctatcgg
       61 cagcttgttg aaggcggtaa tctggacatc tccggttccg ggcgggaagt cgacgatcag
      121 gtagtcgagc tcgccccacc gaacgtcggt ccagaactgc tcgatggcct tggacaggag
      181 cgggccgcgc cagacgaccg gcgtgttctc gtcgccgacg tagaagttga tggagacgac
      241 cttgatgccg tcctcgctcg ttatcggcac gagcatcgag ccgtcggact cgccgcggaa
      301 cgacgagagg cccaggagcc tcggcacgct cgggccggtg atgtccgcgt cgaggaggcc
      361 gaccttcgcg ccgcgagcgg cgagcgcttc ggcgagcagc accgcgacgg tcgacttgcc
      421 gacgccgccc ttgccgctcg agacgccgat gacctgcttg accttggctc cgccgttatt
      481 ctccacggcc tggccctgag agcttgaatc ctgcatgcga accttcctga aacggcgcct
      541 cgcgggcgcc ggtgattgga tctgtttatc cgaggcctat tatatacgcc tgggcgaccc
      601 atgttaagtg ggcatatgct tatatttcca ggaaagctcc atttgacgcg ggctggatag
      661 tcgaatactc ttcatgcgtg agctatgagc tgacgtccga gcatgagaag atcctatcgt
      721 cgctcctacg tttcgttacc gtagcgggag tcctggcgta cctgcccggt acggtcgtcg
      781 gtttcctcga aagcctctgg gccgtcgtgg cggtcaatac gatagcctac gccacagtcg
      841 cggccgtcgc cttcatcccg ggcgtcggct acagggctaa gctgaccgtc ctcgtcgggg
      901 cttgcctcgc cgtcgggtcg gtcgtgctcg tatctaccgg gccgctcggg gctggctaca
      961 tctggctgct cgtcggcgtg gtgctgtcgg ccttgttcgg ccggaaacga gccgtggcgc
     1021 tgaccatagg ggctacgatg gccatcatga tcggctgggc tgtcgccctt ggggtcggcg
     1081 ccgacggacg aggcgccaca ccctggaacg tcggcattat cgccgcgagc ctcctcgtca
     1141 tctgcctcgc cctgtcgttc atcatcaggc gcctacaggg tagcctggcg tcggccttcg
     1201 aggataaggc ccggctggcc gacagcctgg cggccgagct ccaggagtcc catagcacca
     1261 aggataggct cgaatcgacc ctcggcgaga tggacgagct cctccgggaa ctccagcacc
     1321 gtgtcagaaa taacctgcag accgtcgcta gccttctggc gctcgacgag cgcccgccga
     1381 ccagtcgggc ccagggtccc gacgagccgc tggagcgcgc tcggcgcaga atacgcgctc
     1441 tgacggtagc caacgactgc ttcctcgacg gcggggacgg cggcagggcc gatgcctggg
     1501 gcctgatccg ctcggcggcc cagtcggccg acgaggacca tcgcggctgc gattgtaggc
     1561 tgctccgtac ggatcggggc gagccgacga tcgatccgca cgcggctccg ctcgtggcca
     1621 tactcgtcag cgacgtcgcg gctagcctcg gctcgctcgg caggatggct ttggtcgtca
     1681 agcctggcga cggcgtcgtc gtcgaggcgc gctgcctcgc cccgggagag aaccccgagg
     1741 cgcgggtcgt ctatcgcgcc atcgcctcgg gccgggtcgc acggagcgcc gagcccgaga
     1801 tcaggctctc tttcatcgag cccgatgacg gctcggggcc gggcatacgc ctggaatacc
     1861 gcgagggtcg ggtcagtact tgaagcctag agccgcgccg gccacgttca agactagcgc
     1921 agcgacgtaa ggaacggccg cgatagtcgc cttgcccttg gtcgaaccgc gcgtgatggc
     1981 caggat
//
LOCUS       DAOOLN010000298         1982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_72217_length_1982_cov_3.641412, whole genome shotgun sequence.
ACCESSION   DAOOLN010000298 DAOOLN010000000
VERSION     DAOOLN010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72217_length_1982_cov_3.641412"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..1654)
                     /locus_tag="PLW80_04650"
     CDS             complement(173..1654)
                     /locus_tag="PLW80_04650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPB65826.1"
                     /translation="MRRRLLASFRSRMITASLAPIILMGAAATFNLAVSSANLARVAN
                     LFSLDLFIEEALISVDAVVTNLAVYIDTKNSDALRACMHHSKLLSAKAGVLAEGLSYD
                     EDAIIERNLAVLLRRLIEESEAAIRAKRGRNIPEYEARYQDVRMLASFIRDRADALSL
                     ERLGLNLDAFESLAKDGGRAATLLGAMLASAIAFAVAIVLSYSLKLTEPLDALAAAAG
                     RISTGDFSGGPLAASADDEIGQTARAFNVMQASIAAYIDEIRNKAAVESSLMEERLKN
                     LEMQGHLRSAELKALQSRINPHFLFNTLNAGIQLATVEDADRTRVFLERLAGLMRYSF
                     RDLDAPVALRDELACLRDYAYLMTIRFPESIDFDISAEPGAEAAALPKMIIQPLVENS
                     IRHGFADKPLGGAVRVRASLDGGDLVVEVEDNGSGIPDSRAAEIVAAADAGTELGSAE
                     GGLGMANVIARLRLFAGRREVIEFANAVPGARVVVRVPFKEAF"
     gene            complement(1651..>1982)
                     /locus_tag="PLW80_04655"
     CDS             complement(1651..>1982)
                     /locus_tag="PLW80_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPB65827.1"
                     /translation="ASEAIVRELLAEHASIDVAVFIGARDSEGAARALIEYDRVGELA
                     IIGFDDDPALLELVHLGVVAASIARSPERSGEVAVEAIMTLAKGGRVSAYIDPGLTVI
                     RRREGER"
BASE COUNT          304 a          677 c          672 g          329 t
ORIGIN      
        1 cgcacgtcca tcaggatcac gtcgggacgc tcccggacgg cgccgtccac cgcgtctcgc
       61 ccggtgccgg cggtgccgca gatgtccgcg tcgggcaaac gcttccggat cgcggccgcg
      121 acgccatcga tgaccggtcg ctcgtcgtcc gctatgaata tcttggccgt catcagaacg
      181 cctccttgaa cggtacgcgc acgaccaccc tggcgcccgg caccgcgttc gcgaactcga
      241 tcacctcccg cctgccggcg aacagcctga gccttgcgat cacgttagcc atgccgaggc
      301 ctccctcggc ggaaccgagc tcggtgccgg cgtcggccgc cgcgacgatc tcggcggctc
      361 gggagtccgg gatgcccgac ccgttgtcct ctacctcgac gacgaggtct ccgccgtcga
      421 ggctcgcccg cacgcgtacc gcgccgccga gcggcttatc ggcgaaaccg tggcgtatgg
      481 agttctcgac gagcggctgg atgatcatct tcggcaaggc ggccgcctcc gccccgggtt
      541 cggccgagat gtcgaagtcg atcgactcgg ggaagcgtat ggtcatcagg taggcgtagt
      601 cgcgcaggca ggccagctcg tcgcgcagcg ccaccggcgc gtccaggtcg cggaaactgt
      661 agcgcatgag ccccgcgagg cgctcgagga acacgcgcgt gcggtccgcg tcctcgacgg
      721 tcgccagctg gatgcccgcg ttcagcgtgt tgaacaggaa gtgcgggttg atcctcgact
      781 gcagcgcctt gagctcggcg ctgcgtaggt gaccctgcat ctcgaggttc ttgaggcgct
      841 cctccatcag cgacgactcg accgcggcct tgttccgtat ttcgtcaata taggcggcta
      901 tgctcgcctg catcacgttg aaggctcggg cggtctggcc tatctcgtcg tcagccgagg
      961 ccgccagcgg gccgccggag aagtctccgg tcgatatcct accggccgcg gcggccagcg
     1021 cgtcgagcgg ctcggtcagc ttgagcgagt agctcaagac tatggcgacg gcgaaggcta
     1081 ttgccgaggc gagcatcgcg ccaagcaggg tggcggcccg gccgccatcc ttggccaggg
     1141 actcgaacgc gtccaggttg aggccgagcc gttcgagcga cagggcgtcg gcccggtcgc
     1201 ggatgaacga ggcgagcatc ctcacgtcct gatacctcgc ctcgtactct ggaatgttgc
     1261 ggcctcgctt ggcgcggata gcagcctcgc tctcctcgat caggcggcgc agcaacaccg
     1321 cgaggttgcg ctcgatgatg gcgtcctcgt cgtacgatag cccctcggcc aacacgccgg
     1381 ccttggccga gagcagcttc gagtggtgca tacaggcgcg cagggcgtcg gagtttttcg
     1441 tgtctatgta gacggcgagg ttggtgacga cggcgtcgac gctgatcagc gcttcctcga
     1501 tgaacaggtc gagtgagaag aggttggcca ccctcgcgag gttggccgac gacacggcca
     1561 ggttgaaggt cgccgccgcg cccatcagga tgatcggggc caggctagcg gtgatcatgc
     1621 gcgagcggaa cgacgccagg agccgccgcc tcatcgttcc ccctcccgtc ggcgtatgac
     1681 ggtcaggccc ggatcgatgt aggcgctcac ccggcctccc ttggcgagcg tcatgatcgc
     1741 ctcgaccgcg acctcgccgg agcgctcggg cgagcgggcg atggacgcgg cgacgactcc
     1801 gaggtggacg agctcgagca gggccgggtc gtcgtcgaag ccgatgatgg ccagctcgcc
     1861 gacccggtcg tactcgatca gggcccgagc ggcgccttcc gagtccctgg ccccgatgaa
     1921 cacggccacg tcgatcgagg cgtgctccgc cagcagctcc ctgactatcg cctcgctcgc
     1981 cg
//
LOCUS       DAOOLN010000299         1982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_72219_length_1982_cov_3.619097, whole genome shotgun sequence.
ACCESSION   DAOOLN010000299 DAOOLN010000000
VERSION     DAOOLN010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72219_length_1982_cov_3.619097"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PLW80_04660"
     CDS             complement(<1..433)
                     /locus_tag="PLW80_04660"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HPB65828.1"
                     /translation="MLLFALIIVAVIGSYIIPAGEFDRATDPNSGRTVVVPGTFHRVE
                     QNPQHPFKTFIALQKGSIDAADVVFFVFFVYASFYIVLQTGALHSFIGGLLRALRGKE
                     VVLIPVFMYIFALGGSVFGMFEETFGFIPLFAGPASGTGRTV"
     gene            664..1548
                     /locus_tag="PLW80_04665"
     CDS             664..1548
                     /locus_tag="PLW80_04665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HPB65829.1"
                     /translation="MLTNFHTHCEFCDGKATAAAMADAARRAGFSILGFSSHAPLPFV
                     TRWTMKQASLGDYVDAIRGLGRTHGPDMRILVGLEVDSIEGLCGPADGRFDGAGLDYR
                     IGSVHFVLPDGAPAPTPEALDETGEPTFGFTVDESEEKFDDHLSRFYDGDAEAMVDDY
                     YAAVAACARAGGFDILGHFDLVRKNNRGQRRFSEEGRRYRDAAMAAVEAVAESGAIVE
                     INTGGMARGKTDSPYPAAWILRELKARGVGVCLNADAHETRHLSAHREDGLRAAAAAG
                     YDRLTVIGPDGRFQVELG"
     gene            complement(1602..1958)
                     /locus_tag="PLW80_04670"
     CDS             complement(1602..1958)
                     /locus_tag="PLW80_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HPB65830.1"
                     /translation="MDKDFTEKMRESLTEMKQDILDKLVATNADFRAIVEEMDPKDFA
                     DVASDDVDRKMIEALGSQDLKRLRSIEAALVRIQQGRYGSCMKCSKRIPQERLVALPY
                     AVLCIECQKSEERRNR"
BASE COUNT          384 a          629 c          616 g          353 t
ORIGIN      
        1 gcacggtacg accggtgccg gacgctggac cggcgaacag cgggatgaag ccgaatgtct
       61 cctcgaacat accgaagacc gagccgccga gcgcgaagat atacatgaag acggggatca
      121 gcacgacttc cttgccccgt agcgccctca ggagccctcc gatgaacgag tgcagcgccc
      181 cggtctgcaa cacgatatag aacgaggcgt agacgaagaa cacgaagaac acgacgtccg
      241 ccgcgtcgat cgagcccttc tgcagggcta tgaaggtctt gaagggatgc tggggattct
      301 gctcgacgcg atggaacgtg ccggggacga cgaccgtacg cccggaattt ggatccgtgg
      361 ctcgatcgaa ttcaccggcc gggatgatgt acgaaccgat gacggcgacg atgatcaagg
      421 cgaacaaaag cacgaaggta tgcggtactt tgaacggttt tttttactca tgcgcgagcc
      481 tcccgtagcg gatgtgtatg gcgccaagcg ccatgtttcc tcgcacagca acaccggcta
      541 caatccgggt caatcatttt ctgcatatcc atgcgcacat ggcccctttt ttgcatatct
      601 gaagcatatt tgccgcgcca cccttcgttg cctatgggag gcctgccgcg ctatactccg
      661 cccatgctga ccaatttcca tacccactgc gaattctgcg acggcaaggc caccgccgcc
      721 gctatggcgg acgccgcgcg ccgggcgggc ttctcgatac tcggattctc gtcccacgct
      781 ccgctgccgt tcgtaacgag gtggacgatg aagcaggcca gcctcggcga ctacgtcgac
      841 gcgatacgcg ggctggggcg gacgcacggc cccgatatga ggatcctcgt cgggctggag
      901 gtcgattcta tcgaagggct atgcggcccg gctgacggcc gcttcgacgg cgcgggcctt
      961 gactaccgca tcggctcggt ccatttcgtc ctgccggacg gagcgccggc cccgaccccg
     1021 gaggccctcg acgaaaccgg cgagccgacc ttcggcttca ccgtggacga gtcggaggaa
     1081 aagttcgacg accacctctc gcgcttctac gacggcgacg ccgaagccat ggtcgacgac
     1141 tactacgcgg ccgtggccgc ctgcgcgcgc gctggcggct tcgacatact tgggcatttc
     1201 gacctggtcc gcaagaacaa ccgcggccag aggcgcttca gcgaggaggg gcgccgctac
     1261 agggacgcgg cgatggcggc ggtggaggcg gtcgccgaga gcggggcgat cgtggagatc
     1321 aataccggcg gcatggcgcg gggcaagacc gactcgccct accccgcagc ctggatactg
     1381 agagaattga aggcgcgcgg cgtaggcgta tgcctgaacg ccgatgccca tgagacccgc
     1441 cacctgagcg cgcatcgcga agacgggctt cgggcggcgg cggccgcggg ctatgacaga
     1501 ctgaccgtca taggcccaga cggccgcttc caggtcgagc tcggttaaac ctagcgcttg
     1561 tcgcaaccta gcgcttgtcg caacctagcg cttgtcgcaa cctagcggtt gcggcgttcc
     1621 tcgctcttct ggcactcgat gcaaagcacg gcgtatggca gggccacgag ccgttcctgg
     1681 ggaatgcgct tcgaacactt catgcacgaa ccatagcggc cctgctggat acggaccagg
     1741 gcggcctcga tcgagcgtag ccgcttcagg tcctgggagc cgagcgcctc tatcatcttc
     1801 ctgtcgacat cgtcggaggc tacatcggcg aaatccttag gatccatctc ctcgacgatc
     1861 gcccggaaat cggcgttcgt cgcgacaagc ttatcgagga tatcctgttt catctcggtc
     1921 agcgattcgc gcatcttctc ggtgaaatcc ttgtccatcg gaccctcctc gatactcaag
     1981 cg
//
LOCUS       DAOOLN010000300         1976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_72516_length_1976_cov_4.127017, whole genome shotgun sequence.
ACCESSION   DAOOLN010000300 DAOOLN010000000
VERSION     DAOOLN010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72516_length_1976_cov_4.127017"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1976)
                     /locus_tag="PLW80_04675"
     CDS             complement(<1..>1976)
                     /locus_tag="PLW80_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65831.1"
                     /translation="REANLLSARGDIVFERRRADGSDYFVGEALELDMDDWSGAFLDG
                     ESKRAAGEESGDALFFRADDIVKRGSDVLVFKDGVVSSCDDESPHYSIRASRIWILGG
                     NEWAMLNATLSVGELPLLYLPFFYYPGEEIAFNPVFGYEERFGRYVQTTTYLVGRKEV
                     KEQEISLLKIAEGGSGYERRIDGVFLRTTREPAKSKTTDFVKVIVDLYSNLGGFAAVQ
                     AQLASPGPFSSTTAFAGIGLSRSVFGAADGYTPFVSAGDYASVWNGVDLLGTELPFRF
                     GWELASSLSAGPVKLSFSLPFYSDAYYNVDFRDRSEQMNWLQFLDQDDDETTVSKLSS
                     FTDSLSLSASLPSASLPSWLSTASLTKLNTSLAWNSVVKPTPSDSTEAKLFAVDPTRE
                     FFAPYEWTVLDSSASLSGSLFKYPKKAGKAAPTASGPTAAASATDARGGEGGEGAERV
                     PDAVDRALAALPSLEAPWAASAALDKAEKAEKAAVPGFSAPALASPETYADRTPLSAS
                     MSWSLSPTFAWKRRFLTTAWTEPELVDWSALYETRTSRAAGSVNLVGAAYDGLLALTL
                     GLSASSQAQDRPSISSDAAYASESLKTTWAKQDAQYRNDKLSAQLKLTSSPFQDLWLW
                     SPTSFGYSLSSTLYEYAFKEMDATAPTDASKAVY"
BASE COUNT          316 a          680 c          671 g          309 t
ORIGIN      
        1 gtagacggcc ttcgaggcgt ccgtcggggc cgtcgcgtcc atctccttga acgcgtactc
       61 gtacagcgtc gacgagaggg agtagccgaa gctggtcggc gaccagagcc acaggtcctg
      121 gaacggggag ctcgtcagct tgagctgcgc gctcagcttg tcgttacggt actgcgcgtc
      181 ctgcttggcc caggtcgtct tgagcgactc gctcgcgtag gccgcgtccg acgagatgct
      241 cggcctatcc tgggcctggc tcgacgccga caggccgagc gtcagggcga gcagaccgtc
      301 gtaggccgcg cctaccagat tgaccgagcc agccgccctc gaggtcctcg tctcgtacag
      361 cgccgaccag tccacgagct ccggctcggt ccaggccgtg gtcaggaagc gccgcttcca
      421 ggcgaaggtc gggctgagcg accacgacat cgaggcggat agcggcgtac ggtcagcgta
      481 ggtctcgggc gaggccaggg ccggcgcgga gaagccgggg acggccgcct tctccgcctt
      541 ctcagcctta tccagcgccg cggaggcggc ccagggcgcc tcgaggctcg gcagggccgc
      601 gagcgcccgg tcgacggcat ccgggacgcg ctcggcgcct tcgccgcctt cgcctcctcg
      661 ggcatccgtc gcggaggccg cggccgtcgg gcccgaggcc gtgggggcgg ccttcccggc
      721 cttcttcggg tacttgaaca gcgagcccga caggctggcg ctcgagtcga gcaccgtcca
      781 ctcgtacgga gcgaagaact cgcgcgtcgg gtcgaccgcg aacagcttgg cctcggtcga
      841 gtcgctcggc gtcggcttga ccaccgaatt ccaggccagc gaggtgttca acttggtcag
      901 cgacgcggtc gacagccacg acggcaggct ggccgagggc agcgacgccg acaggctgag
      961 cgaatccgtg aaggacgata gcttcgatac cgtcgtctcg tcgtcgtcct ggtcgaggaa
     1021 ctggagccag ttcatctgct cgctgcggtc acggaagtcc acgttgtagt acgcgtcgct
     1081 gtagaacggc agcgagaagc tcagcttgac cggcccggcc gacaggctcg aggccagctc
     1141 ccagccgaag cggaacggca gctccgtacc gagcagatcc acgccgttcc agaccgacgc
     1201 gtagtcgccg gccgagacga agggcgtgta gccgtcggcc gcgccgaaga ccgagcgcga
     1261 tagccctatc cccgcgaagg ccgtcgtcga cgagaacggg ccgggcgagg cgagctgggc
     1321 ctggaccgcc gcgaagccgc ccaggttcga gtacaggtcg acgatcacct tgacgaagtc
     1381 ggtcgtctta gacttcgccg gctcccgcgt cgtccggagg aacaccccgt cgatcctgcg
     1441 ctcgtagccc gagcctccct cggctatctt gagcaggctg atctcctgct ccttgacctc
     1501 cttgcggccg accaggtagg tcgtcgtctg cacgtagcgg ccgaagcgct cctcgtagcc
     1561 gaagaccggg ttgaacgcta tctcctcgcc cgggtagtag aagaacggca ggtacaggag
     1621 cggcaattcg ccgaccgaca gcgtcgcgtt gagcatggcc cactcgttgc cgccgagtat
     1681 ccagatcctg gaggcgcgga tcgagtaatg cgggctctcg tcgtcgcagg agctgaccac
     1741 gccgtccttg aacaccagca cgtcgctgcc gcgcttgacg atgtcgtcgg cccggaagaa
     1801 cagcgcgtcg ccggactcct cgccggccgc ccgcttgctc tccccgtcga ggaaggcccc
     1861 gctccagtcg tccatgtcca gctcgagcgc ctcgccgacg aaatagtccg agccgtccgc
     1921 cctgcggcgc tcgaagacga tgtcgccgcg ggccgacagc aggttggctt cgcgat
//
LOCUS       DAOOLN010000301         1970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_72877_length_1970_cov_3.757702, whole genome shotgun sequence.
ACCESSION   DAOOLN010000301 DAOOLN010000000
VERSION     DAOOLN010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72877_length_1970_cov_3.757702"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1860)
                     /locus_tag="PLW80_04680"
     CDS             complement(<1..1860)
                     /locus_tag="PLW80_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65832.1"
                     /translation="MASALPALRAVPDGPGDHLADWREIRRRYELFMGARGLYEAAWL
                     GRSASRPVARWVLLYPDLTEDWDDYAEAVLALPGSSAILSDRLGAEPVRAARFGTAVE
                     EIRAVLLAIRDEVASGADPAGIVISAAAPESTLPLLEREAAVAGVPLDVREGRPLSES
                     SGGRLLADAKALADSGMSFEALRRLLLDASIPWKEAEAARRLLAIGVRAHIVAPLPGG
                     RDVWEASIGDDEPARRLYRGLRTASSRIAGSTDFRSLRAAFDAFKRAFLDEALWSPRQ
                     DDEMARCLAALDELDEAASTAGLERVPGAVDAWLEQLSETRYVPASTSGGIAVYRFPV
                     AAGAMPDLHFVVNLAQGAAVAASRPLAFMRADERARLGAADRDLSAGLVRLLARSGGR
                     VFMSYSEDGPDGVRPPHPAVYAVPPESSGLAYERDRWLPNLEADGRAIAEAFPTQAAC
                     ARAAAVTVVSPSSPDWSGATPDAPATMGRWATEAVRASMSRDGRLRLSATAIEGYASC
                     AFRRVFARTLKVEAMESGLSFIDSLLVGGIYHDAFGRLFRPLAAAGRTIVAPEAAGEE
                     ARPSGADVDAAIAAAITGAGAELGPMAAVLVSTAKPALRRDFARAVAALVPALD"
BASE COUNT          287 a          678 c          765 g          240 t
ORIGIN      
        1 atccagggcc gggacgaggg cggcgaccgc gcgagcgaag tcccggcgca gagccggctt
       61 cgcggtcgat acgagcacgg cggccatcgg cccgagctcg gcgccggcgc cggttatggc
      121 ggcggctatg gcagcgtcga cgtcggcgcc cgagggacga gcttcctcgc cggcggcttc
      181 cggcgcgacg atcgtgcggc cggccgccgc cagcggcctg aaaaggcggc cgaacgcgtc
      241 atggtagatg ccgccgacga gcaagctgtc gataaacgac aggccggatt ccatagcttc
      301 gaccttcagc gtccgcgcga agacccggcg gaaggcgcag gaggcgtagc cctcgatcgc
      361 cgtggccgat agcctgaggc ggccgtcgcg gctcatggag gcacggacgg cctcggtggc
      421 ccaccggccc atcgtcgccg gggcgtcggg cgtcgctccg gaccagtccg gcgaggacgg
      481 tgaaacgacg gtgacggccg ccgcccgagc gcaggccgcc tgggtcggga aggcctcggc
      541 tatcgcccgg ccgtcggcct cgaggttcgg tagccagcgg tcgcgttcgt aggcgaggcc
      601 cgacgactcg ggcgggacgg cgtagacggc ggggtggggc ggccgcacac cgtccggccc
      661 atcttccgag tagctcatga agacgcggcc gccggatcgg gccaggagcc gcacgagtcc
      721 ggctgacagg tcgcggtccg ccgcgcccag cctggcccgc tcgtcggccc gcatgaacgc
      781 tagcggcctc gaggcggcga cggccgctcc ctgggccagg ttgacgacga agtgcaggtc
      841 gggcatcgcg ccggcggcca ccgggaagcg gtacaccgcg ataccgcccg aggtggatgc
      901 cggcacgtag cgcgtctcgg acagctgctc gagccacgcg tccacggcgc ccggtacgcg
      961 ctcgagcccg gcggttgacg ccgcctcgtc gagctcgtcg agcgcggcca gacaccgggc
     1021 catctcgtcg tcctggcgcg gcgaccagag cgcctcgtct aagaaggcgc gcttgaacgc
     1081 gtcgaacgcc gcgcggaggg atcggaagtc ggtcgagccc gctatcctcg aactggcggt
     1141 cctgaggccg cggtacaggc gccgcgccgg ctcgtcgtcg cctatcgagg cctcccagac
     1201 gtcgcgccct ccggggagcg gcgcgacgat atgggctcgt acgcctatgg cgagcagcct
     1261 gcgggcggcc tcggcctcct tccaggggat ggaggcgtcg aggagaaggc gtctgagcgc
     1321 ctcgaaagac atgccgctgt cggccaaggc cttggcgtcg gcgagtagcc ggccgcccga
     1381 gctctcggac agcggccggc cctcgcgcac atcgagcggc actccggcga ccgcggcctc
     1441 gcgttcaagc aggggcaggg tcgattctgg cgcggcggcg ctgatgacga tgccggccgg
     1501 gtcggcgccg gaggcgacct cgtcgcgtat ggcgagtagc acggcgcgga tctcctcgac
     1561 cgcggtgccg aacctcgcgg cgcggaccgg ctctgcgccg aggcggtcgg acagaatggc
     1621 ggatgagcct ggcagggcga gtacggcttc ggcgtagtcg tcccagtcct cggtcaggtc
     1681 cgggtacagg agcacccagc gggctacggg acgcgatgcg gagcggccga gccaggcggc
     1741 ctcgtagagg ccccgcgcgc ccatgaacag ctcgtagcgt cggcgtatct ctcgccagtc
     1801 ggccagatgg tcgcccggac cgtccggcac ggcgcgtagg gctgggagag ccgacgccat
     1861 ggagcgcgcg aacctgagcg acgaggcggc cgactgggcg ggtatgacgg agcgtaggaa
     1921 gggcgagcga gcgttttcgg ccgcgagcgc ccgcgcgaag acggcgcgca
//
LOCUS       DAOOLN010000302         1970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_72899_length_1970_cov_2.323760, whole genome shotgun sequence.
ACCESSION   DAOOLN010000302 DAOOLN010000000
VERSION     DAOOLN010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72899_length_1970_cov_2.323760"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="PLW80_04685"
     CDS             <1..631
                     /locus_tag="PLW80_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008882824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPB65833.1"
                     /translation="TPIREAFKLADDVLRQGVQGISDLITVAGDINIDFADVSTVMSG
                     QGDAIMGIGYARGDNRAVEAAEKAVSNPLLEDARIDGAKNILINVTGGESFTLTEYEE
                     IVSIITRNADPNALVISGIVSDDASDDEVSVTVIATGFDRSIKPLAPSAPARKDVTKE
                     LDDFLSADEWRTIVEPKQKGYLMGRNDADELDIPTVLRERRPASERSGV"
     gene            635..1585
                     /locus_tag="PLW80_04690"
     CDS             635..1585
                     /locus_tag="PLW80_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HPB65834.1"
                     /translation="MAGRDSIDRYGAWLLAARRRSPLTLEAYVREARSLASWLGERGL
                     AVETALVTDILEYLAYRRSSGLSARTMARIMSGIRGFFKYLRLEGLRSDDPTELLESP
                     RQERSLPDVIRADDVNLMLGSIDVATPGGLRDRALFELIYSCGLRISEAASLTFDSLY
                     LKERFIRVVGKRRKERILPFGDEARSWLTRYLEEGRPALEKGRRSDRVFLNRSGAGIS
                     RKGVWKRFSELRDASGVDGKVHSLRHSFATHLLAGGADLRTVQELLGHADISTTQIYT
                     HVDAEDLAVAHAAYFPRSGDRAQGASAEARDGVSDTGRAS"
     gene            1585..>1970
                     /locus_tag="PLW80_04695"
     CDS             1585..>1970
                     /locus_tag="PLW80_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65835.1"
                     /translation="MRRTIILALAAVATLSSCGGDDATFIERMFDLESRSSKGAPPST
                     IEELKEGIRRYGDEVEKTAAAMEKVGTYWRMLAVRYLDKGLYGDAYDAATKALRHYPD
                     SSGIYYVAGMSAAFLSFTLSIDDVVMS"
BASE COUNT          349 a          659 c          646 g          316 t
ORIGIN      
        1 caccccgata cgcgaggcct tcaagctggc cgacgacgtg ctcaggcagg gcgtgcaggg
       61 catctccgac ctcatcaccg tcgccggcga catcaacata gacttcgccg acgtcagcac
      121 cgtgatgagc gggcagggcg acgcgatcat gggcataggc tacgcccgcg gcgacaaccg
      181 cgccgtcgag gcggccgaga aggccgtatc caacccgctc ctcgaggacg cccgtatcga
      241 cggcgccaag aacatcctca tcaacgtgac cggcggcgag agcttcacgc tgaccgagta
      301 cgaggagatc gtcagtatca tcacgcggaa tgccgacccc aacgctctcg tcatctccgg
      361 tatcgtcagc gacgacgcgt cggatgacga ggtcagcgtc acggtcatcg ccaccggctt
      421 cgaccgcagc atcaagccgc tggcgccgag cgcgccggcg cgcaaggacg tgaccaagga
      481 gctggatgac ttcctgtccg ccgacgagtg gcgcaccatc gtcgagccta agcagaaagg
      541 ctacctgatg ggccgcaacg acgccgacga actcgatatt cccaccgtgc tacgcgagcg
      601 ccgtccggcc tcggagcgga gcggggtcta gacggtggcg ggccgggatt cgatagaccg
      661 ctacggcgcc tggctcctcg ccgccaggag gcgctcgccg ctaacgctcg aggcctacgt
      721 ccgcgaggcg cgctcgctgg cctcgtggct gggcgagcgc ggcctggccg tcgagacggc
      781 gctcgtgacg gatatactcg aatacctcgc gtaccgccgc tcatccggcc tctcggccag
      841 gacgatggcc aggatcatgt cgggcatacg cggcttcttc aagtacctgc gcctcgaggg
      901 gctgcgctcc gacgacccga ccgagctcct ggagagcccc aggcaggaac gcagtctgcc
      961 ggacgtcatc cgcgccgatg acgtcaatct gatgctcggc tcgatagacg tcgcgactcc
     1021 gggcggtctg cgcgaccgcg cgctgttcga gctgatctac tcctgcggcc tgcgcatctc
     1081 ggaggcagcg tcgctgacct tcgattccct gtacttgaag gaacgcttca tccgcgtcgt
     1141 cggcaagcgg cgcaaggagc gtatcctgcc cttcggggac gaggcccgtt cgtggctgac
     1201 ccgctacctg gaggagggcc ggccggcgct cgagaagggc aggcggagcg accgcgtctt
     1261 cctcaaccga tccggcgccg gtatctcgag gaagggcgtc tggaagcgct tctccgagct
     1321 gcgcgacgcg agcggcgtcg acggcaaggt gcactcgctc aggcattcct tcgccacgca
     1381 cctcctagcc ggcggtgccg acctacgaac cgtgcaggag ctactcgggc acgcggatat
     1441 ctcgacgact cagatctata ctcacgtcga cgcggaggac ctcgcggtcg cccacgcggc
     1501 gtatttccct cgatccggcg accgcgccca aggggcaagc gccgaggctc gggatggcgt
     1561 ctccgatacg gggagggcaa gctgatgagg cgaacgataa tcctggccct ggcggcggta
     1621 gcgacgctgt catcgtgcgg cggcgacgat gcgactttta tcgagcgcat gttcgacctg
     1681 gagtcgaggt catccaaggg cgctcctccg tcgaccatcg aggagctgaa ggaaggcatc
     1741 cgacggtacg gcgacgaggt cgagaagacc gccgccgcga tggagaaggt cggcacctac
     1801 tggcgcatgc tcgccgtccg ttatcttgac aagggcctgt acggcgacgc gtacgatgct
     1861 gcgacgaagg cgctcaggca ctatccggat tcatccggca tctactatgt agctggcatg
     1921 tcggcggcct tcctctcgtt cacgctgtcc atcgacgacg tggttatgtc
//
LOCUS       DAOOLN010000303         1970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_72905_length_1970_cov_1.791123, whole genome shotgun sequence.
ACCESSION   DAOOLN010000303 DAOOLN010000000
VERSION     DAOOLN010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72905_length_1970_cov_1.791123"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PLW80_04700"
     CDS             complement(<1..319)
                     /locus_tag="PLW80_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65836.1"
                     /translation="MNICLPVRFEHRTELTRDDRAAVEALARECLDSDGFDPCLHYDT
                     HLNADRDLRPWRLAWACAAGGDDANGGDDASGGDDNANGDDNANGGDDANGGDDANGG
                     DDAN"
     gene            complement(316..>1970)
                     /gene="uvrB"
                     /locus_tag="PLW80_04705"
     CDS             complement(316..>1970)
                     /gene="uvrB"
                     /locus_tag="PLW80_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPB65837.1"
                     /translation="DINAEIERHRLSATRSLMERRDVIIVATVSCIYGLATPEVYREM
                     KVEAAVGEELDPVAFKRQLVSLQYERNDSVLERGRFRVRGDVIEVYPAYANEAYRIEL
                     DFDRVERIRRFDPVSGQAVEELDAAVIYPAKQFVMPETMVQRALSSIKEELAERYEEL
                     MAKRRLVEAQRLKTRVEYDIELLEEMGYCPGIENYSGPLSGRARGQRPSVLLDYFPDD
                     FLTFIDESHVTLPQVGAMYAGDRSRKQNLVDYGFRLPCALDNRPLKFDEFEGLTRRVI
                     YVSATPGVAEVGKSARVVEQMIRPTGLVDPEILVRPSEGQMEDIYAEVKRRIALGERS
                     LILTLTKRMAEELSEYLSSLSLNVRYIHSEVETIERVEILTQLRTGEYDVLVGINLLR
                     EGIDLPEVSFIAILDADKIGFLRSATSLVQIIGRAARNASGVVVMYADRMSDAMRVAI
                     DETNRRRAVQQAYNEAHGITPTTVKKAIHDILQRHKEEKEQALDAELETVKGAYNLLV
                     PEQKAALVKRLEQEMLERAKNLEFEAAAILRDEIERIKAGES"
BASE COUNT          282 a          645 c          664 g          379 t
ORIGIN      
        1 cattagcgtc gtcgccgcca ttagcgtcgt cgccgccatt agcgtcgtcg ccgccgtttg
       61 cgttgtcgtc gccgtttgcg ttgtcgtcgc cgccgcttgc gtcgtcgccg ccattagcgt
      121 cgtcgccgcc cgcggcgcag gcccaggcca ggcgccaagg gcgcaggtct cggtcggcat
      181 tcaggtgggt atcgtagtgc aggcacgggt cgaagccgtc gctatcgagg cattcacggg
      241 cgagcgcctc gacggcagcg cggtcgtccc gcgtcagctc cgtcctatgc tcgaatcgaa
      301 ccggcaggca gatgttcacg actcgcccgc cttgatgcgc tcgatctcgt cgcggagtat
      361 cgcggcggcc tcgaattcga ggttcttggc ccgctcgagc atctcctgct caagccgctt
      421 gacgagggcg gccttctgct cgggcacgag caggttgtag gcgcccttga ccgtctcgag
      481 ctcggcgtcg agcgcctgtt ccttctcctc cttgtggcgc tggaggatgt cgtggatggc
      541 cttcttgacg gtggtcgggg tgataccatg ggcttcgttg taggcctgct ggaccgcgcg
      601 gcggcggttc gtctcgtcga tggccacgcg catggcgtcc gacatccggt cggcgtacat
      661 gacgacgacg ccggaggcgt tgcgcgcggc gcggccgatg atctgtacga ggctggtggc
      721 cgagcgcagg aatccgatct tgtcggcgtc gagtatcgct atgaacgaca cctcgggcag
      781 gtcgatgcct tccctgagca ggttgatgcc gacgagcacg tcgtactcgc cggtccgtag
      841 ctgggtgagt atctcgacgc gctctatcgt ctccacctcg ctgtggatgt agcgcacgtt
      901 gagcgagagg gacgagaggt actcggacag ctcctcggcc atacgcttgg tcagcgtgag
      961 gatcaggctg cgctccccga gcgcgatgcg gcgcttgacc tcggcgtaga tgtcctccat
     1021 ctgcccctcg ctcgggcgta cgagtatctc cgggtcgacg aggccggtcg ggcgtatcat
     1081 ctgctcgacg acgcgggccg acttgccgac ctcggcgacc ccgggcgtcg ccgacacata
     1141 gatgacgcgc ctggtcaggc cctcgaactc gtcgaacttg agcggccggt tgtcgagggc
     1201 gcagggtagc ctgaagccgt aatcgacgag gttctgcttg cgcgaacggt cgcccgcgta
     1261 catcgcgccg acctgcggca gggttacgtg cgactcgtct atgaaggtca ggaagtcgtc
     1321 ggggaagtag tcgagcagca ccgacgggcg ctgcccccgc gcccgccccg acagcggccc
     1381 ggaatagttc tcgatgccgg ggcagtaccc catctcctcc agcagctcga tgtcgtattc
     1441 caccctcgtc ttaagccgct gggcctcgac gaggcggcgt ttggccatga gttcctcgta
     1501 gcgctcggcg agctcctcct tgatggacga cagggcgcgc tggaccatgg tctccggcat
     1561 cacgaactgc ttggccgggt agatcaccgc cgcgtcgagc tcctcgaccg cctggcccga
     1621 taccgggtcg aagcggcgta tgcgctccac ccgatcgaag tccagctcga tgcgatacgc
     1681 ctcgttggcg taggccgggt agacctcgat cacgtcgccg cgcacccgga agcgaccccg
     1741 ttcgagcact gagtcgttgc gctcgtactg gagcgacacg agctggcgct tgaaggcgac
     1801 cgggtcgagc tcctcgccga cggccgcctc gaccttcatc tctcggtaga cctcgggcgt
     1861 ggccaggccg tagatgcagg atacggtcgc cacgatgatc acgtcgcggc gctccatcag
     1921 gctgcgagtg gccgacaggc ggtggcgctc gatctcggcg ttgatgtcgg
//
LOCUS       DAOOLN010000304         1967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_73059_length_1967_cov_3.018305, whole genome shotgun sequence.
ACCESSION   DAOOLN010000304 DAOOLN010000000
VERSION     DAOOLN010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73059_length_1967_cov_3.018305"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1967
                     /locus_tag="PLW80_04710"
     CDS             <1..>1967
                     /locus_tag="PLW80_04710"
                     /inference="COORDINATES: protein motif:HMM:NF020083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65838.1"
                     /translation="MVLFGERRLGELYDEVRSFPPYADLARADFDAVVGMLAGRFAGS
                     RVRELAGRAYLDEEQGTLRAKEGAAMLLYSAGGSIPDRGYYAMRLADGTRLGELDEEF
                     VFERRVGNSFSFGSQAWRIVDIGDEAVTVAPLDRDADFMPFWKAEKAARGASIARRML
                     GLCAAFDADPDALAAELRGRLGFSPEAAKALVAFLSSQSRAQRGERLPSPGSVVIEAF
                     RDPEAKAGATALIVHTLRGSAVNEPLGLALSSLLRDRLGVPVERLSNDDSVSLSMPLV
                     DRDEAVRALVDALRSLADPGTLSRALRGALVGSAIFGAAFRENAGRALLLPRAGFGRR
                     TPLWVTRLRAKRLLERTSGYDGFPIAAETWKTVLEQRFDLASLSALCAGLADGSVAAP
                     IFSPAAPSPFARQCGWAETNRYLYEDDESTISSSGTPAGGAAGAAEAAITRAMSDPAA
                     RPRVDPALASAYGARLRRELPGWAPSSPDGLADWVDERLAIPLDEWDALLAVCPEGLR
                     EAARAAIAAPGTGPGLLSRLEALTLPGASEAVVVRREGKAALLESAGRPDGRASLAAR
                     WLPYAGPVTLGRLGCLFGPGAAEPGLLEAAGVVVGPGLGVQPRSRSGEPAVEAPSVSA
                     HAADAPGACDRDVLESLLRQGRAAARPKVRA"
BASE COUNT          229 a          775 c          693 g          270 t
ORIGIN      
        1 cgatggtgct gttcggcgag cgccgccttg gcgagctata cgacgaggtt cgctccttcc
       61 cgccctacgc ggacctcgcg cgggccgact tcgacgccgt cgtcggcatg ctcgccggcc
      121 gcttcgccgg tagccgcgtg agggagctag ccggccgcgc ctacctcgac gaggagcaag
      181 ggacgctccg ggccaaggaa ggcgcggcga tgctcctcta ctcggccgga ggctccatac
      241 ccgaccgcgg ctactacgcg atgcgcctgg ccgacggcac gaggctcggc gagctcgacg
      301 aggagttcgt cttcgagcgc cgcgtcggca acagcttcag cttcggctcc caggcgtggc
      361 ggatcgtcga cataggcgac gaggcggtca ccgtggcccc gctcgaccgc gacgccgact
      421 tcatgccctt ctggaaggcg gagaaggccg cccgcggcgc ctccatagct cgccgcatgc
      481 tcgggctgtg cgccgccttc gacgccgacc cggacgccct cgccgccgag ctacgcggcc
      541 ggctcggttt ctcgcccgaa gccgccaagg ccctcgtcgc gttcctgtcg tcgcagtcgc
      601 gggcccaacg gggtgagagg ctcccttcgc cgggctcggt ggttatagaa gccttccgcg
      661 acccggaagc caaggccggc gccaccgcgc tgatcgtcca taccctgcgc ggctcggccg
      721 tcaatgagcc gctcggcttg gccctgtcgt cgctgctccg cgacaggctc ggcgtgccgg
      781 tcgagcgcct atcgaacgac gactcggtat cgctgtccat gcccctcgtc gaccgggacg
      841 aggccgtacg cgccctcgtg gacgcgctcc gttcgctcgc cgacccgggg acgctgtcca
      901 gggctctgcg cggcgcgctc gtcggcagcg ctatcttcgg cgcggccttc agggagaacg
      961 ccgggcgcgc cctcctcctg ccgcgagccg gcttcggcag gcgcaccccg ctctgggtca
     1021 cgaggctccg cgccaagcgc ctcctcgagc gcacgagcgg ctacgacggc ttccccatag
     1081 ccgccgagac ctggaagacg gtgctcgagc agcgcttcga cctcgcctcg ctgtcggccc
     1141 tctgcgcggg cctggccgac ggctccgtcg ccgccccgat cttctcgccg gccgcgccca
     1201 gtcccttcgc gcgccagtgc ggctgggccg agacgaacag gtacctctac gaggacgacg
     1261 agtcgacgat atcctcttca gggactccgg cgggcggggc cgccggagcc gccgaggcgg
     1321 ccattaccag ggcgatgagc gacccggccg cccgcccgcg cgtcgacccc gcgctcgcgt
     1381 cggcctacgg cgcgaggctc aggcgcgagc tgcccggctg ggcgccgtct agccccgacg
     1441 gcctcgccga ctgggtcgac gaacggctgg ccataccgct cgacgaatgg gacgccctgc
     1501 tcgccgtctg cccggagggc ctgcgcgagg ccgcccgggc cgcgatcgcc gccccgggga
     1561 ccggccccgg gctcctgtcc aggctcgagg cgctgacgct gcccggcgcc tcggaggccg
     1621 tcgtggtcag gcgagagggc aaggccgcct tgctggagtc cgcgggccgc ccggacggcc
     1681 gggcgtcgct cgcggcccga tggctgccct acgccggccc cgtaacgctc ggccggctcg
     1741 gctgcctgtt cgggccgggc gcggccgagc ccgggctcct cgaggcggcc ggcgtcgtcg
     1801 tcggccccgg cctcggcgtc cagccccgct cgcgatccgg cgagccggcg gtcgaagctc
     1861 cctcggtcag cgctcacgcc gccgacgccc cgggcgcctg cgaccgcgac gtgctcgaat
     1921 cgctcctcag gcagggccgc gccgccgcca ggcccaaggt acgggca
//
LOCUS       DAOOLN010000305         1957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_73626_length_1957_cov_3.149317, whole genome shotgun sequence.
ACCESSION   DAOOLN010000305 DAOOLN010000000
VERSION     DAOOLN010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73626_length_1957_cov_3.149317"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /gene="scpB"
                     /locus_tag="PLW80_04715"
     CDS             <1..328
                     /gene="scpB"
                     /locus_tag="PLW80_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPB65839.1"
                     /translation="EGLKDRYGRKNEQKLSRAALETLSIIAYSQPVTRAEVEAIRGVA
                     ADTMIRFLLDKGFIKEVGKKDAPGKPIQYGTTKDFLKFFRLGSISDLPKLDELERDRF
                     ELEGRA"
     gene            325..1056
                     /locus_tag="PLW80_04720"
     CDS             325..1056
                     /locus_tag="PLW80_04720"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPB65840.1"
                     /translation="MSSGEDGTRIQALIARAGLASRRAAEECIRAGRVRLNGRVVTEL
                     GTKALPSDRLTLDGKPVVAEERIHHLALNKPPGYICAMRDDYGRPLAASLFKPAIAER
                     VYNVGRLDLESCGLILFTNDGEFAARVGHPSSGVVKEYLVETDKPVPADFARRFVAGI
                     EDDGETLRALEATVTGERRCSIRLVEGRNREIRRALKGFGLRATLLRRVAIGDVRLGD
                     LAEGAWRSLSAKEVAGLMATMEGRR"
     gene            1053..>1957
                     /gene="cmk"
                     /locus_tag="PLW80_04725"
     CDS             1053..>1957
                     /gene="cmk"
                     /locus_tag="PLW80_04725"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPB65841.1"
                     /translation="MIVAMDGPAGTGKSTIARMVAERRGFTYINSGNLYRAITYGAMR
                     SGVPLDDEAALTSYAEGAGLEYRGGALYLDGEPIEGRLRSAEVDAVVAQVSAIVPIRH
                     IVNRLIRSIGAGLDAVVEGRDICTVVYPDAEVQIYIDASAEARARRRFDQGTSSLSLD
                     EIKKNIEMRDRIDMNKAEGSLKRAPKAFYLDTSYLTIDQVYDIVDSKIQQQGNPMAKE
                     VEMGAVAPSDDSIQTQLQEQYLKTMNHLEEGYLVEGQVVQVASDCVFVDVGAKSEGKI
                     PLSEFTEPPKVGDVVTVVLMKKETRS"
BASE COUNT          404 a          588 c          654 g          311 t
ORIGIN      
        1 ggaggggctc aaggaccgct acggccgcaa gaacgagcag aagctgtcta gggcggctct
       61 cgagaccctg tcgatcatcg cgtactccca accagtgacc agggctgagg tcgaggccat
      121 acgcggcgta gcggcggaca ccatgatacg cttcctgctc gacaagggct ttatcaagga
      181 agtcggcaag aaggacgcgc cgggaaagcc catccagtac ggcaccacga aggatttcct
      241 caagttcttc aggctcggca gcatctccga cctgcccaag ctcgacgagt tggagcgcga
      301 tcgcttcgaa ctcgagggac gggcgtgagc tcgggcgagg acggtaccag gatacaggcg
      361 ctcattgccc gcgccggcct ggcctcgcgc cgggccgccg aggaatgtat acgcgccggc
      421 agggtccggc tgaacggccg cgtggtcacc gagctgggta ccaaggccct gccttcggat
      481 cgcctgacgc tcgacggcaa gccggtcgtc gccgaggaac gtatccacca tctcgcgctc
      541 aacaagccgc cgggctacat ctgcgccatg cgcgatgact acggccggcc gctcgccgcg
      601 tcgctgttca agccggctat agccgagcgg gtttataacg tcgggcggct cgacctggaa
      661 tcgtgcgggc tcatcctgtt cacgaacgac ggcgagttcg ccgcgagggt aggccacccg
      721 tcgtcgggcg tcgtcaagga gtacctcgtc gagaccgaca agcccgttcc ggccgacttc
      781 gcgcggcgtt tcgtcgccgg gatcgaagac gacggcgaga ccctgcgggc actcgaggcg
      841 accgtcacgg gcgagcgtcg ctgctcgatc aggctcgtcg agggcaggaa ccgcgagatc
      901 aggcgggcgc tcaagggctt cgggctcagg gcgacgctcc tacggcgcgt ggccatcggg
      961 gacgtgcggc tcggcgacct ggccgaggga gcttggcgca gcttgagcgc gaaagaggtc
     1021 gctggcctca tggcgaccat ggagggacga cgatgatcgt ggcgatggac gggccggcgg
     1081 gaaccggtaa aagcacgata gccagaatgg tggccgagcg ccgaggcttt acctatatca
     1141 actcgggcaa cctgtatcgc gccataacct acggcgccat gcgctcgggc gtgccgctcg
     1201 acgacgaggc cgccttgacg tcctatgccg aaggcgccgg actggaatac cgcggcggcg
     1261 ctctctacct ggacggcgag ccgatcgagg gccggctacg gagcgccgag gtggacgcgg
     1321 tcgtggccca ggtctcggcg atcgtgccca tacggcatat cgtcaaccgg ctgatccgct
     1381 ccattggcgc cggcttggac gccgtcgtcg agggccggga catctgcacc gtcgtctatc
     1441 cggacgccga ggtgcagatc tacatcgacg ccagcgccga agccagggcc aggaggcgct
     1501 tcgaccaggg tacgagctcg ctaagtctcg atgagatcaa gaagaatata gagatgcgcg
     1561 ataggataga catgaataag gcggaaggta gccttaaaag ggcgccgaaa gccttctatt
     1621 tagatacgtc atacttgacc atagatcaag tttatgacat tgtggactcg aaaattcaac
     1681 agcaagggaa ccctatggcc aaggaagtgg aaatgggcgc ggtcgctccc tccgacgaca
     1741 gcatccagac acagttacag gaacagtatc taaaaaccat gaaccacctg gaagagggtt
     1801 acctcgtcga gggacaggtg gtacaggtcg ccagcgattg cgtcttcgtc gacgttggcg
     1861 ccaagagcga gggcaagatc ccgctctccg aattcacaga gccgccgaag gttggcgacg
     1921 tggtgaccgt ggttctcatg aagaaagaga ccaggtc
//
LOCUS       DAOOLN010000306         1955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_73736_length_1955_cov_2.867895, whole genome shotgun sequence.
ACCESSION   DAOOLN010000306 DAOOLN010000000
VERSION     DAOOLN010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73736_length_1955_cov_2.867895"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PLW80_04730"
     CDS             <1..201
                     /locus_tag="PLW80_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65842.1"
                     /translation="PEKRGELAAIASAIAEAGGNLLSLVAFDGDDGKTGYCTLKVEGV
                     AKDALVSVVEPLVERVMEAREG"
     gene            353..1081
                     /locus_tag="PLW80_04735"
     CDS             353..1081
                     /locus_tag="PLW80_04735"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HPB65843.1"
                     /translation="MTVTELLEAISPPRPLPGAAARTVMAPPFRRQELEALACLGDPK
                     PTVPSRAGWREAAVLVLFYESGGGLRFPLIVRPDGPGVHAGQVSLPGGAREPEESPED
                     CALRETREEIGVDPGSVRLVRALTPLHVPPSRFLVRPFAGVAAARPEFRLSGSEVAVL
                     LEPRLDELLDPAGASCVDVRLGVAAGVGDGIADGIADGVAVGVAVGVAEAPCYELAGY
                     SVWGATAMILSELRAMIVGELAAG"
     gene            complement(1095..>1955)
                     /locus_tag="PLW80_04740"
     CDS             complement(1095..>1955)
                     /locus_tag="PLW80_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPB65844.1"
                     /translation="TRTFVDALRSFRDGVDAADAATEDQKRFVAETSAAMEEMAASIR
                     SISAMTERAGTLSGRLSESGDAGSAAVRDTRAAIDEINAAAREVLGVLTTLNKISADI
                     NLLAMNAAIEAAHAGEHGQGFAVVADEVRTLAGNAAQRTRAIKELIQAMGARVDNGVR
                     QAEASGAALAGLTAGLGESASISREIAAAAKEQAAGTDAVIGSITQAVRSADTVSAMM
                     GDQARRSDEIAMALEGVLRRLEGLAADASRQTREVTALEESFAAVRKEVDGNLESVGA
                     LGSEMARFSV"
BASE COUNT          244 a          692 c          702 g          317 t
ORIGIN      
        1 cccgagaagc gcggcgaact ggcggcgatc gcttccgcca ttgccgaggc gggcggcaac
       61 ctgctgtccc tcgtcgcctt cgacggcgat gacggcaaga cgggttactg cacgctgaag
      121 gtcgaaggcg tggcgaagga cgctctcgtt tccgtcgtcg agccgctcgt ggagcgcgtc
      181 atggaggcgc gggagggcta gggcctcgtc cgggctcatg ccgcggaatc gttccgcata
      241 gtcgtcggcg aggccgcgcg cgcaggggta gacgaggtcc tgggcgcggc cgagcgcttc
      301 cctgggcgcg gctcccttgc cccatcggtc cgctcggggt catactcgaa gcatgaccgt
      361 gaccgaactg ctcgaagcca tctccccgcc caggccgctg ccgggggcgg cggcccgaac
      421 cgtgatggcg ccgccgttcc gccggcagga gctcgaggcg ctcgcgtgcc tcggggatcc
      481 aaagcccacc gttccgtcca gagccgggtg gcgagaggcg gcggtgctgg tactgttcta
      541 tgagagtggc ggcggactac gcttcccgct gatcgtcagg ccggacggac cgggcgtcca
      601 cgccggacag gtatcgctac cgggcggggc gcgcgagcct gaagaaagcc ccgaggactg
      661 cgccctacgc gagacgcgcg aggagatcgg ggtggacccc ggctcggtcc gcctggtacg
      721 ggcgctgact ccgctccatg tcccgccgag caggttcctg gttcggccct tcgccggcgt
      781 cgcggccgcg cgccctgaat tccgcctgtc cggcagcgaa gtcgcggtgc tgctcgagcc
      841 gcggctcgac gagctgctcg atccggccgg agcgtcctgc gtcgacgtac gcctcggcgt
      901 cgcggccggc gtcggggacg gcatcgcgga cggcatcgcg gacggcgtcg cggtcggcgt
      961 cgcggtcggc gtcgccgaag ccccgtgcta cgagctagcc ggctattcgg tctggggcgc
     1021 caccgcgatg atcttgtccg agctgcgcgc gatgatagtt ggcgagctgg cggccggtta
     1081 gccgcccagt cgggctagac cgagaacctg gccatctccg agccgagcgc gccgaccgat
     1141 tcgaggttgc cgtcgacttc cttcctgacg gccgcgaacg actcctcaag cgcggtcacc
     1201 tcgcgcgtct ggcggctggc gtcggccgcg agcccctcga gtcgccgtag cacgccctcg
     1261 agggccatcg cgatctcgtc gctacgcctg gcctggtcgc ccatcatcgc gctgaccgta
     1321 tcggccgatc tgaccgcctg cgtgatggag cctatcacgg cgtccgtgcc ggcggcctgc
     1381 tccttagcgg cggcggctat ctcccgcgag atcgaggcgg actcgccgag gccggcggtg
     1441 agcccggcca gggccgcccc tgaggcctcc gcctgccgta cgccgttgtc gacgcgcgct
     1501 cccatggctt ggatcagctc cttgatggcg cgagtacgct gggccgcgtt cccggccagg
     1561 gtacggacct cgtcggcgac cacggcgaag ccctgcccgt gttctcccgc gtgggccgcc
     1621 tcgatcgcgg cgttcatagc gagtaggttg atgtcggccg atatcttatt gagtgtcgtc
     1681 agcacgccga gcacctcgcg ggcggcggcg tttatctcgt ctatcgcggc cctggtgtcg
     1741 cgcaccgcgg cgctaccggc gtcgccgctc tcggaaagcc gcccagacag cgttccggcc
     1801 cgctcggtca tggccgagat cgagcggatg ctcgcggcca tctcctccat cgcggccgag
     1861 gtctcggcca cgaagcgctt ctggtcctcg gtggcggcgt cggccgcgtc cacgccgtcc
     1921 ctgaaggagc gcagcgcgtc gacgaaggtc ctggt
//
LOCUS       DAOOLN010000307         1952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_73889_length_1952_cov_3.235108, whole genome shotgun sequence.
ACCESSION   DAOOLN010000307 DAOOLN010000000
VERSION     DAOOLN010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73889_length_1952_cov_3.235108"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(298..1299)
                     /gene="argF"
                     /locus_tag="PLW80_04745"
     CDS             complement(298..1299)
                     /gene="argF"
                     /locus_tag="PLW80_04745"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPB65845.1"
                     /translation="MPVNLKGRSFLTLKDFSPAEIRYLLDLSHELKAKKRAGIRGDLL
                     DRKNIVLIFDKASTRTRCAFETACWDEGGNATFLTNSQMGKKESVEDTAKVLGRMYDG
                     IQYRGFSQKLVTDLAKHSGVPVWNGLTDEFHPTQILADLLTIEEHVKKPLNKVKLVYC
                     GDARNNMGNSLMIGSAKMGMHFVALAPKELWPNEKLTAEMRDVAKSTGAVIEFFDISK
                     ADEALKGADALYTDVWVSMGEESKFAERIKQLAAYRVTMDFIRKTGNPDCIFLHCLPA
                     FHDQETEVGRQVGKEHGVMEQEVTDEVFRSRHSVVFDEAENRLHTIKAVMVATIGRQ"
     gene            complement(1479..>1952)
                     /locus_tag="PLW80_04750"
     CDS             complement(1479..>1952)
                     /locus_tag="PLW80_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB65846.1"
                     /translation="RTAEVMTADGWFRTGDLGALDAKGRLFIKGRLKTMILGASGENI
                     YPEEIEAMINKSAYVAESLIVPDGNGLTALVQLKDEVVAELKAKAQDRIEDFERASEE
                     LLEFVRKEVNQGLAAFSKVSKVKLHHEPFEKTPTQKIKRFLYTFKGPSPVGNDGK"
BASE COUNT          303 a          612 c          653 g          384 t
ORIGIN      
        1 ctgggactcg gcgtctacgg cccggccctc gacgagcggg gcaactccgc cggcggcctc
       61 gccgcgctca ggctcctatc cgacgaactg gagctgcggg tgctgtgatc cgccgccgcc
      121 tgcgaccctc ggcgtacgat cggtttggag actgccgaac cgagccaggg ctgatacggc
      181 aggcgcgcca tagtatccga gccagataga acgttaaaaa gaacggccgc cccgaggata
      241 cggggcggcc acctagccgg agcgagtcga gcccggcccc ggtcagaacg ggttcgccta
      301 ctgcctaccg atggtcgcca ccatcacggc cttgatcgtg tgcaggcggt tctcggcttc
      361 gtcgaagacc acggagtggc ggctgcggaa gacctcgtcg gtcacttcct gctccatgac
      421 gccgtgctcc ttgccgacct ggcggccgac ctcggtctcc tggtcgtgga aggccggcag
      481 gcagtggagg aagatgcagt ccgggttgcc ggtcttcctg atgaagtcca tcgtcacgcg
      541 ataagccgcg agctgcttga tgcgctcggc gaacttagac tcctcgccca tcgacaccca
      601 gacgtcggta tacagcgcgt cggcgccctt cagggcctcg tcggccttgg agatgtcgaa
      661 gaactcgatc acggcgccgg tcgatttggc cacgtcccgc atctcggcgg tcagcttctc
      721 gttcggccac agctccttgg gggccagcgc gacgaagtgc atgcccatct tggcggagcc
      781 tatcatcagg gagttgccca tgttgttgcg ggcgtcgccg cagtagacca gcttgacctt
      841 gttgagcggc ttcttgacgt gctcctcgat ggtcaggagg tcggccagga tctgggtcgg
      901 gtggaactcg tcggtcaggc cgttccagac cggtacgccc gagtgcttgg ccaggtcggt
      961 cacgagcttc tgggagaacc cgcggtactg gatgccgtcg tacatgcggc cgagcacctt
     1021 ggccgtgtcc tcgaccgact ccttcttgcc catctggctg ttcgtcagga aggtggcgtt
     1081 gccgccttcg tcccagcagg ccgtctcgaa ggcgcagcgc gtgcgggtgg aggccttgtc
     1141 gaagatgagg acgatgttct tgcggtcgag gaggtcgccg cggattccgg cgcgcttctt
     1201 ggccttgagc tcgtgggaca ggtcgaggag ataacggatc tcggccgggc tgaagtcttt
     1261 gagggtcagg aagcttcttc ctttgagatt gacgggcatg ggttcctcct gtactaagtg
     1321 aacgagcggg cgatcggtac gtcgcgcgcg cggtcggcgg cgaacgcccg accgtagctg
     1381 cggtatagca ccgcccccgg agcgaggtca agggacgggg cgtatccgct taaggcgcat
     1441 aaaaataccg gctccgggcc ccgttcccgc cggaagagct acttcccgtc gttacccacg
     1501 ggcgacgggc ccttgaacgt atacaagaag cgtttgatct tctgggtcgg cgtcttctcg
     1561 aacggctcgt ggtgcagctt caccttggac accttgctga aggcggccag cccctggttg
     1621 acctccttgc ggacgaattc gagcagctcc tcgctcgctc gctcgaagtc ctcgatcctg
     1681 tcctgcgcct tggccttgag ctcggctacg acctcgtcct tgagctggac gagggccgtg
     1741 agcccgttgc cgtcgggcac gatcagcgat tcggcgacgt aggccgactt gttgatcatg
     1801 gcctcgatct cctcggggta gatgttctcg ccggaggcgc cgagtatcat cgtcttgagg
     1861 cggcccttga tgaacaggcg gcccttagcg tcgagggcgc cgaggtcgcc ggtacggaac
     1921 cagccgtcgg cggtcatgac ctcggcggtg cg
//
LOCUS       DAOOLN010000308         1951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_73949_length_1951_cov_2.940928, whole genome shotgun sequence.
ACCESSION   DAOOLN010000308 DAOOLN010000000
VERSION     DAOOLN010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73949_length_1951_cov_2.940928"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..1464
                     /locus_tag="PLW80_04755"
     CDS             22..1464
                     /locus_tag="PLW80_04755"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB65847.1"
                     /translation="MRLRTATRSAAALAVILLASCAGLGSRSGPTEPPSLPGAAGTLV
                     PAELSAEEKERMALDALGRAMDSGDYESALGRLVELVELDPGNRSYPALRSALCLSLG
                     RTDEARALLSAELAAYPDNVEALYALAELERFVGNAGAHRAAIEAIVARHPGDSDALT
                     SLGDIHYDAKNYRKAEESYDAALAAEPGNAEALKGLARVKFRRDDAKGALALLDKAVA
                     AAPDDAIAYLDRSRALYELGRYAECEADMDKAVELAPGSSWNYVERGRLYLDTGRLEL
                     AEADLSESIRLNPDYFLPYVYRASIRENSGDDEAALADYKAAARLYPDYWYSLESMGV
                     LAYRLGDWKTSFASFDGAAGYTKAHPEYYVAAALSLYRGGDARAAKEYAGKKLPLIDR
                     GKHQVHWLALRLVYDQGEMTAEAELAISAEKSLDIKAGALFYLGSYWIAKGRQELGLK
                     YLRLSYDSERIGTIERRMAEADLARLAAGD"
     gene            1486..>1951
                     /locus_tag="PLW80_04760"
     CDS             1486..>1951
                     /locus_tag="PLW80_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB domain-containing protein"
                     /protein_id="HPB65848.1"
                     /translation="MSDTIKALELSGRRIVLVGTAHVSRESVDEVAATIRDEAPDGVC
                     VELDEARWRSMSAPDAWEKTDIVKVIKEGRAFLLLANLALSSFQRRMGMDSGVKPGAE
                     MVAAIRAAEAAGIPFGFCDRDVQTTLRRAWGKSNLWNKSKLLAQLVAAAFSNE"
BASE COUNT          329 a          663 c          666 g          293 t
ORIGIN      
        1 aaccctcgac cggagagacg catgagacta cgaacggcga cgcgctcggc cgccgccctg
       61 gccgttatac tcctcgcttc ctgcgcgggg ctcgggagcc gatccgggcc gacggagcct
      121 ccgtcgctac ccggcgccgc cggcacgctc gtcccggccg agctgagcgc cgaggaaaag
      181 gagcggatgg ccctcgacgc gctcggccgc gccatggatt cgggcgacta cgagtccgcc
      241 ctcgggcgtc tcgtcgagct ggtcgagctg gatcccggca accgctcgta tccggcgcta
      301 cgctcggcgc tatgcctgtc gctcggtcgg accgacgagg ctcgcgcctt gctctcggcg
      361 gagctggccg cctacccgga taacgtcgag gctctgtacg cgctggcgga gctcgagcgc
      421 ttcgtcggaa acgccggggc ccaccgcgcc gcgatcgagg ccatcgtcgc gaggcatcca
      481 ggagattccg acgcccttac ctccctcggc gatatccatt acgacgcaaa gaattaccga
      541 aaggccgagg agtcgtacga cgcggcgctc gccgccgagc caggcaacgc cgaggccctc
      601 aagggtttgg ctcgggtcaa attccgcagg gacgacgcga aaggcgccct tgccctgctc
      661 gacaaggccg tcgcggcagc accggacgac gcgatcgcct acctggaccg cagccgcgcc
      721 ctctatgagc tgggccgcta cgccgagtgc gaggccgaca tggacaaggc cgtcgagctc
      781 gcgcccggct cgtcctggaa ctacgtcgag cgcggccggc tctacctgga taccggccgc
      841 ctcgaactcg cggaggcgga tctctccgag tccatccgcc tgaatccgga ttatttcctg
      901 ccctacgtct acagggcgtc gatacgcgag aattccggcg acgatgaggc ggcgctcgcc
      961 gattacaagg cggcggcccg gctgtacccc gattactggt actcgctcga gtcgatgggc
     1021 gtgctcgcgt accggctcgg cgactggaaa acgtccttcg cgtcgttcga cggagcggcc
     1081 ggttatacca aggcgcatcc cgagtactat gtggccgcgg ctctgtcgct gtaccggggc
     1141 ggcgacgcga gggccgccaa ggagtacgcg ggcaagaagc tgccgctcat agaccgcggc
     1201 aagcatcagg tccactggct ggcgctgcgc ctcgtctacg accagggcga gatgacggcc
     1261 gaggcggagc tggcgatcag cgccgagaag agcctcgaca tcaaggccgg cgcactgttc
     1321 tacctgggct cctactggat agccaagggc cggcaggagc tcggcctcaa gtacctgcgc
     1381 ctatcgtacg actccgagcg tatcggcacg atagagcgcc gcatggccga ggccgacctg
     1441 gcccggctgg cggccggcga ttgaatccgc acgaacggag tagcgatgtc tgatacgatc
     1501 aaggcgctcg agctgtcggg gcgcaggata gtgctggtgg gcaccgccca cgtctcccgg
     1561 gagagcgtcg acgaggtggc cgcgacgata cgcgacgagg cgcccgacgg cgtctgcgtc
     1621 gagctcgacg aggcgcgctg gcgctcgatg agcgcgccgg acgcctggga aaagaccgac
     1681 atcgtcaagg tgatcaagga gggcagggct ttcctgttgc tcgcgaacct ggcgctgtcg
     1741 tccttccagc gcaggatggg catggactcc ggcgtcaagc caggagccga gatggtcgcc
     1801 gccatacgcg ccgccgaggc cgccggcatc ccgttcggtt tctgcgaccg cgacgtccag
     1861 acgacgctgc gcagggcctg gggcaagtcg aatctctgga acaagtcgaa gctcctggcc
     1921 cagctggtcg cggccgcctt ctcgaacgag a
//
LOCUS       DAOOLN010000309         1948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_74151_length_1948_cov_2.353407, whole genome shotgun sequence.
ACCESSION   DAOOLN010000309 DAOOLN010000000
VERSION     DAOOLN010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74151_length_1948_cov_2.353407"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1678)
                     /gene="dnaG"
                     /locus_tag="PLW80_04765"
     CDS             complement(<1..1678)
                     /gene="dnaG"
                     /locus_tag="PLW80_04765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPB65849.1"
                     /translation="MRRIPDHVVQDIVSRTDIVRVVGEYVRLEKRGSRWVGLCPFHNE
                     KTPSFGVNEERGFFFCFGCKKGGDAITFIKEVEKCGYVEALEMLAEKAGVVISYEGDD
                     DPEEARAAKERESLYELYERLAGTFRHFIVADSRGAEALAYARRRGLPDEVIASFRLG
                     YAPAERSWLYRFLRGKSYSPEFLARSGLFSKKYPETAIFSDRLMFPIADARGRVVAFG
                     GRLLHGDGPKYINSPETGIFKKHDTLFALPEAAQAMRTSSTAILCEGYMDAIAFHAAG
                     VANAVAPLGTSFTEPQAALLKRYASTVVLCFDTDGAGIAAAERAIGIAESKGLGVRVL
                     RLEGAKDPAEILEKNGPERLKKNAVSTITSEDFILENAASRLRAGDSGSKTEAFEYLF
                     PFLANLSSDIRRDSLMVAASSRLGADPASVRSDFIRFLDKGSIRRHVQAPVPVASDDF
                     APSADAELLAAVIANAELFEAARSEIGPADFDDESLKDAFIVMEECYRSGDQQVATVV
                     ERLTSAGLKEFILQRISDGAYSINPDRFVLDGVRRVKLRALEARKQRVVSR"
     gene            complement(1675..>1948)
                     /locus_tag="PLW80_04770"
     CDS             complement(1675..>1948)
                     /locus_tag="PLW80_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPB65850.1"
                     /translation="TERQGKPHPSRIFDRDLRIEDPYNTYKHPGLPPSPICNPGLTAI
                     SAAIRPAVSGYLYFRLVDEATGEHYFSATLDEHVRAASLAVKPIGR"
BASE COUNT          359 a          600 c          618 g          371 t
ORIGIN      
        1 tacgcgatac gacgcgctgt ttcctggcct cgagcgcccg gagcttgact cggcggacgc
       61 cgtcgaggac gaagcggtcc ggattgatcg agtacgcccc gtcggatatc ctctgcagta
      121 tgaactcctt gagccccgcg ctcgtcaggc gctcgacgac ggtggctacc tgttggtcgc
      181 cgctcctata gcactcctcc atcacgatga acgcgtcctt gagcgactca tcgtcgaaat
      241 ccgccgggcc tatctccgag cgcgcggcct cgaacagctc cgcgttggct atgacggcgg
      301 ccaaaagctc ggcgtcggcg ctcggggcaa aatcgtccga ggctacaggg acgggagcct
      361 ggacgtgtct ccgtatcgac cccttatcca agaatcgtat gaaatcgctc ctgactgaag
      421 cgggatctgc gcccaaacga gaggacgcgg ccaccatgag cgagtctcgc cgtatatcag
      481 acgatagatt cgccagaaat gggaaaagat attcgaacgc ctccgtttta gaccccgaat
      541 cacccgctcg aagccgagaa gcggcatttt ccaatataaa atcttcggaa gttatagtgg
      601 aaacggcatt ctttttcaat cgttcaggac catttttttc cagtatctcg gcgggatcct
      661 tggcaccctc gagcctgagc acccggacgc ccaggccctt ggactccgcg ataccgatag
      721 ccctctcggc ggcggcgatc ccggcgccgt cggtatcgaa gcagaggacg acggtcgacg
      781 cgtaacgctt gagcagggcg gcctgtggct cggtgaacga cgtcccgagc ggggctacgg
      841 cgttggcgac ccctgccgcg tggaacgcta tggcgtccat atacccctcg catagtatgg
      901 ccgtcgagct ggtcctcatc gcctgcgcgg cctccggcag ggcgaacagc gtgtcgtgct
      961 tcttgaagat acccgtctcc ggcgagttga tgtacttggg gccgtctccg tgcagcagtc
     1021 tgcctccgaa ggcgacgacc cggcccctgg cgtccgctat cgggaacatc aggcggtcgg
     1081 agaagatggc cgtctccggg tactttttcg agaagaggcc ggagcgagcc aggaattcgg
     1141 gcgaatacga cttgccgcgc aggaagcgat agagccagga tcgttcggcc ggtgcgtagc
     1201 cgagccggaa cgaggcgatc acctcgtcgg gcagacccct gcgccgagcg tacgcgagcg
     1261 cctcggcgcc gcgcgagtcg gctacgatga aatgcctgaa ggtgccggcg aggcgctcgt
     1321 acagctcgta gagcgactcg cgttccttgg cggcgcgcgc ctcctccggg tcatcgtccc
     1381 cctcgtagga gatgacgacg ccggctttct cggcgagcat ctcgagggcc tcgacgtagc
     1441 cgcatttctc gacttccttg atgaaggtga tggcgtcgcc gcccttcttg cagccgaagc
     1501 aaaagaagaa gccgcgctcc tcgttgacgc cgaatgacgg cgtcttctcg ttatggaacg
     1561 ggcagaggcc gacccagcgc gagccgcgct tctcgagccg cacgtactcg ccgacgactc
     1621 tgacgatatc ggtcctggat acgatatcct ggacgacgtg atccgggatg cgtctcatct
     1681 gcctatgggc ttcacggcga gcgacgcagc cctgacgtgc tcgtcgaggg tggcggagaa
     1741 gtagtgctcg cccgttgcct catcgacgag cctgaaatac aggtagccgg atacagcagg
     1801 ccggatagcg gcggagatgg ccgtaaggcc gggattgcag atggggctag gaggtagccc
     1861 ggggtgctta tacgtattgt acggatcctc gatccgcagg tctcggtcga atatcctgct
     1921 cggatgcggc ttgccttggc gttcggtg
//
LOCUS       DAOOLN010000310         1947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_74207_length_1947_cov_2.361522, whole genome shotgun sequence.
ACCESSION   DAOOLN010000310 DAOOLN010000000
VERSION     DAOOLN010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74207_length_1947_cov_2.361522"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..1486
                     /gene="rseP"
                     /locus_tag="PLW80_04775"
     CDS             128..1486
                     /gene="rseP"
                     /locus_tag="PLW80_04775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPB65851.1"
                     /translation="MTIATIALGLVGLGFVVFFHELGHFIMARLVGVEVEEFSIGMGP
                     RLLSRRFGKTAYTLSSLPIGGYCRMKGEDSFKKAIEQGLNEFPRESGSYFGAAPLKRI
                     LIALGGPFANVVLAFILYAAIAGAGYETRSWENRIVLASDTDGSSYPADRAGLASGDR
                     IVAIGGEAVSSFSEIQEAIALSARKPIPLTVDRHGESFSLVVEPELDAGSGAGRVGVY
                     PWIEPVVETVAPDGAAALAGLRPGDRIVAINGEPAVHGVAIASRLEADRPAKITVAYE
                     RGGFIAETTVVLAYSEERGADLGVGWKSTVSKIQAESLGQAMVMGVDETADTISATYR
                     GIASLFMGVNLLQAVSGPARITWMVGTVAKSGLTDGTVGGFSTLLGFLAILSIGLFAM
                     NLLPIPLLDGGSIALFVVELVRRKAPKVKTVLRYQTIGLVAVAALFLLSTIGDVLFFS
                     GR"
     gene            1521..>1947
                     /locus_tag="PLW80_04780"
     CDS             1521..>1947
                     /locus_tag="PLW80_04780"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HPB65852.1"
                     /translation="MKKIICRCDAEVEVDSPDLLDLDADPAMLGRLARGDSPSAACPR
                     CGAIVRAELPLKVRSASRGVDIVVLPEIERLATYRGKADPTGSAEILLGYQELFERSR
                     MLSDGLDPSCVEALKYALQGKAEETEPEAEISVFYNGVSG"
BASE COUNT          334 a          597 c          656 g          360 t
ORIGIN      
        1 acattgcccg cgtcgtctcg cggaccctgg agcgcgcgta tccgccgacg ctcgaagggc
       61 tcgaggcggt gctggccgtc gacgccgacg ccaggcgcgt cgcccgatcg atagtcaagg
      121 agatctcatg accatcgcca ccatagcgct cggcctcgtc gggctcggct tcgtcgtatt
      181 cttccatgag ctggggcatt ttataatggc caggctcgtc ggcgtcgaag tcgaagaatt
      241 ctccatcgga atggggccac ggctcctctc caggcgcttt ggcaagacgg cgtacaccct
      301 gtcgtccctg ccgattggcg gttattgtag aatgaagggc gaagactcgt tcaagaaggc
      361 catcgaacag ggtctgaacg agttcccccg cgagagcggc agctacttcg gcgccgcgcc
      421 gctcaagcgc atcctcatag cgctcggcgg gcccttcgcc aacgtcgtcc tggctttcat
      481 cctctacgcg gccatagccg gagccggtta cgagacgcgt agctgggaga atcgcatcgt
      541 actggcgagc gacaccgacg gcagctcgta tccggcggac cgggccgggc tggcgagcgg
      601 tgacaggata gtcgcgatcg gcggcgaagc cgtatcgtcg ttctcggaga tccaggaggc
      661 catcgcgctg tccgccagaa agccgatacc gttgacggtc gatcgccatg gcgagtcgtt
      721 cagcctggtc gtcgagcctg agctcgacgc tggctcgggc gctggcaggg tcggcgtgta
      781 tccatggata gagcccgtgg tcgagacggt cgcgcctgac ggggccgccg ccctcgccgg
      841 cctgaggccg ggcgacagga tcgtggctat aaacggcgag ccggctgtcc acggggtcgc
      901 gatagcctcg cggctcgaag ccgatagacc ggccaagata acggtagcct acgagcgcgg
      961 cggtttcatc gccgagacga ccgtagtgct ggcgtactcg gaggagcgcg gcgccgactt
     1021 gggcgtcggc tggaagtcga ccgtctccaa gatacaggcg gagagcctcg gccaggctat
     1081 ggtcatgggc gtcgacgaga cggctgatac cataagcgcg acctatcgag gcatcgcgtc
     1141 gctattcatg ggcgtgaacc tgctccaagc agtctcgggt ccggcgcgta ttacctggat
     1201 ggtcggcacg gtggccaaga gtgggctgac tgatggaacg gttggcggtt tctcgacgct
     1261 gctgggcttc ctggccatac tgtcgatcgg cttgttcgct atgaatctgc tacccattcc
     1321 gctgctcgac ggcggttcga tcgccctgtt cgtcgtcgag ctcgtcaggc gcaaggcgcc
     1381 caaggtcaag acggtgctca ggtaccagac catcggcctg gtcgccgtcg cggccctgtt
     1441 cctactatcg acgataggcg acgtcctgtt cttctccgga cgttagcgat ataccctata
     1501 gtattgtgac ggagggtgcc atgaagaaga taatctgccg atgcgacgcc gaggtcgagg
     1561 tggactcgcc cgatctgctc gacctcgacg cggatccggc catgctgggg cgactggcgc
     1621 gcggcgactc tccttcggcc gcgtgtccac gctgcggcgc gatcgtcagg gcggagctac
     1681 ctttgaaggt gcggtcggcc tcgcgcggcg tagatatcgt cgtgctaccc gagattgaac
     1741 gtttggccac ctatcgcggc aaggccgacc caaccggaag cgccgagata ctgctcggct
     1801 atcaagagct gttcgagcgc tccaggatgc tgagcgacgg cctcgacccc agctgcgtcg
     1861 aggcgctcaa gtacgcgctt cagggcaagg ccgaggagac ggagccggag gccgagatca
     1921 gcgtgttcta taacggggta tcgggcg
//
LOCUS       DAOOLN010000311         1944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_74367_length_1944_cov_3.355744, whole genome shotgun sequence.
ACCESSION   DAOOLN010000311 DAOOLN010000000
VERSION     DAOOLN010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74367_length_1944_cov_3.355744"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PLW80_04785"
     CDS             <1..355
                     /locus_tag="PLW80_04785"
                     /inference="COORDINATES: protein motif:HMM:NF037014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HPB65853.1"
                     /translation="VYDESGAAVADVMTLAGETIEPGKTVTFHHPSLDTRRVTFEPCG
                     EIRPMLGKVMEGGKRLAPAPTMQELRARRAASMRSFDHTFLRFLNPHIYKVSISGRLR
                     DLKLEFVERYRGSKG"
     gene            complement(362..1792)
                     /locus_tag="PLW80_04790"
     CDS             complement(362..1792)
                     /locus_tag="PLW80_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65854.1"
                     /translation="MPDRIALTSALGPIAWLLVPVIALLFVALRLRRRLVYPHELLAD
                     RSGARPTALLLRMMRLYFDTALDAICAVVVGLALAGYPRPAADKGPVVVMDASASMLA
                     GMRGDRPLDEAARLALSYYSQDEAGARRTRLFALGWDPTRRRHSLTDRSALLETAKSP
                     QELAAGLESSESFMSVDYALLAGLARRGYGEVTLLTDDASVEGLGVEIVRLGAKPPRY
                     LLLATAAWDRGSGKSVARFVAAGGAEPDSLWRLGPGGAAARAKPEDYEIRRSPSGFEL
                     ALARPGPWAVRWDGRLLPFVAPGPPEPLRARGTMAERVIAGLKPIGGATGRGVMVVDR
                     GGAGRPGSLSVAAARDESWLLPPRTTLGALVASGLDRRADLALGPAAFASAEAATPFW
                     LARASSTPTADGATGDSRPTRVGDGYLYPDETVVTPPPGEYAPTGRRVVAAAGSPPER
                     RVAVALALALLYGLKAWLGLRRRRAD"
BASE COUNT          285 a          715 c          699 g          245 t
ORIGIN      
        1 cgtctacgac gagtctggcg ccgccgtggc cgacgtgatg acgctcgccg gcgagacgat
       61 agagcccggg aagacggtca ccttccatca tccgtcgctc gacaccaggc gcgttacctt
      121 cgagccgtgc ggcgagatac ggcccatgct cggcaaggtc atggagggtg gcaagcgcct
      181 cgcgccagcc ccgacgatgc aggagctacg cgccaggcgc gccgcgtcga tgcggtcgtt
      241 cgaccacacc ttcctgcgct tcctgaaccc gcacatctat aaagtatcca tcagcgggcg
      301 gctacgggac ctcaagctcg aattcgtcga gcgctaccgg ggctccaagg gctagccgct
      361 ctcagtcggc ccggcgtcgc ctcaggccga gccaggcctt gaggccgtag agcagggcga
      421 gcgccagcgc gacggcgacc ctgcgttcgg gcggcgagcc cgccgccgcg acgaccctgc
      481 gtcccgtggg cgcgtactcg cccggcggcg gcgtgacgac ggtctcgtca gggtacaggt
      541 atccgtcgcc gacgcgcgtc ggcctcgagt cgcctgtcgc cccgtcggcc gtcggcgtcg
      601 acgaggcccg ggcgagccag aagggcgtcg ccgcctcggc cgaagcgaag gccgccggcc
      661 cgagcgccag gtcggcccgc cggtccagcc cggaggcgac gagggcgccg agggtggtcc
      721 tcggcggtag gagccacgac tcgtcccggg cggcggcgac cgacagcgag cccggcctgc
      781 cggccccgcc gcggtcgacg accatgacgc cccgcccggt agcgccgcct atcggcttga
      841 gacccgcgat gacgcgctcg gccatcgttc cgcgagcccg cagcggctcg ggcggcccag
      901 gcgcgacgaa gggcaggagc cggccgtccc atcgtaccgc ccaggggccg ggcctcgcca
      961 aggccagctc gaagcccgag ggggagcgtc gtatctcgta gtcctcgggc ttggcgcgcg
     1021 ccgcggctcc gcccggcccg agccgccata ggctatcggg ctcggcgccg cccgcggcta
     1081 caaagcgcgc gacgctcttg cccgagccgc ggtcccaggc cgccgtcgcc aggagcaggt
     1141 agcgcggcgg cttggcccct agcctgacga tctcgacgcc gagcccctcg accgaggcgt
     1201 cgtcggtaag gagcgtcacc tcgccgtagc ctcggcgagc caggcccgcg agcagggcgt
     1261 agtcgaccga catgaacgac tccgacgatt cgaggcccgc cgccagctcc tgggggctct
     1321 tggccgtctc gagcagggcg ctcctatcgg tcagggagtg cctgcgcctc gtcgggtccc
     1381 aaccgagggc gaagagcctc gtccggcgcg cgccagcctc gtcctggctg tagtacgaca
     1441 gggccaggcg cgccgcctcg tcgagagggc ggtcgccgcg catgcctgcc agcatcgagg
     1501 ccgacgcgtc catgacgaca accgggccct tgtctgccgc tggccgcggg tagcccgcga
     1561 gggccagccc gacgacgacg gcgcagatgg cgtccagggc ggtgtcgaag tagagccgca
     1621 tcattctcag gagcagggcg gtcggcctcg cgccgctccg atcggcgagg agctcgtgag
     1681 gatagaccag cctgcgcctg agcctgagcg ccacgaacag gagcgctatg acaggcacga
     1741 ggagccaggc gattggtccg agcgccgagg ttagcgcaat ccgatcaggc atacagccgc
     1801 tccagcgcgt cccagtagac cggggcccgg ggtgtgccgg gatcgagccg cctgaagaac
     1861 acccgcggga tcgaggccag agcggcgtcc cgcgacgcct ccgcctccgc cgttcccttg
     1921 agctggatgg cttcggcctc ggcg
//
LOCUS       DAOOLN010000312         1941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_74520_length_1941_cov_3.185578, whole genome shotgun sequence.
ACCESSION   DAOOLN010000312 DAOOLN010000000
VERSION     DAOOLN010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74520_length_1941_cov_3.185578"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>228)
                     /locus_tag="PLW80_04795"
     CDS             complement(<1..>228)
                     /locus_tag="PLW80_04795"
                     /inference="COORDINATES: protein motif:HMM:NF017046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65855.1"
                     /translation="GVDEYRALCGADAVKPRSAFGTDLAAARGRCLFTPPYRADAAIE
                     LGEALGIAPGALSAAASNALITSVVAQREIK"
     assembly_gap    229..238
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<239..514)
                     /locus_tag="PLW80_04800"
     CDS             complement(<239..514)
                     /locus_tag="PLW80_04800"
                     /inference="COORDINATES: protein motif:HMM:NF017046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HPB65856.1"
                     /translation="MFEPSVYSGRRRALVAALASRGALDGLVAFPGNNDSPVNYPDNA
                     YRFRQDSCFLYYFGLAEPGLAAAIDLGTGRATLYADALTIDDLVWTGP"
     gene            complement(595..>1941)
                     /locus_tag="PLW80_04805"
     CDS             complement(595..>1941)
                     /locus_tag="PLW80_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB65857.1"
                     /translation="LALVVIVGGAVLSKGKGGKSKGGKRIKAKDHAQVLKEANRRLAQ
                     NPKDPDALQALGEVYYGEQAWDKAFKAYEVLVEVSAGNPDLDEFEINKRYGLCAMKLG
                     RLDESYKGLVLAHAVKADDFETNYNLGYLEFQKRQYEKAIFHLRLAGRQNPEHAMTLR
                     YLGHSLFKVKNFKEALSSLKRAVDLQPDDKESLFAMGECFHEIGQEEQAIRLFTHLRT
                     DPALGPSAALFAGTIHLNQRQYQKAIMDFELGLRHPDIKVEVLVEIKYRLAAAYLKGQ
                     EIAKAVSLLNEIQQIYPNYKDTPAQLAKYKELNSNRNLQVYLLSATSDFITLCRKITL
                     TFFPKAKVKITDISVQKNDWADILAEVETSRWSDVVLFRMIRSTGTIGELVLRDFHAR
                     IKDLKAGKGYCITAGVFSDESKKFVEARLIDLMEKDKLMTLLNNIDSQQKGLLITD"
BASE COUNT          311 a          624 c          610 g          386 t
ORIGIN      
        1 cctttatctc tcgttgcgcg acgacggacg ttatcaaggc gttcgaagcc gccgcggaca
       61 aggctccggg agctatgccg agcgcctcgc cgagctcgat ggccgcgtcc gcccgatacg
      121 gcggcgtgaa caggcatcga cctctcgcgg ccgccaggtc cgtaccgaac gccgacctcg
      181 gcttgaccgc gtcggccccg cagagcgccc ggtactcgtc gacgcccgnn nnnnnnnncg
      241 gtccggtcca gaccaggtcg tcgatcgtca gagcgtcggc gtacagcgtc gcgcggcccg
      301 tgcccagatc gatcgccgcg gccaggccag gctcggccag gccgaaatag tagaggaaac
      361 acgagtcctg cctgaagcgg tacgcgttgt cgggataatt gacggggctg tcgttattgc
      421 ccggaaaggc gacgaggccg tccagggcgc ctcgcgacgc cagggccgcg accagggcgc
      481 gcctgcgccc ggaatatacc gatggttcga acatggagcc atgctacccc tcgtccggcg
      541 gcgcgtcaac gacgccgcgt caacgacgac gcgtcaacgg acggagcctc gcgatcagtc
      601 cgttatcagg agccccttct gctgcgagtc gatgttgttg aggagcgtca tcagcttgtc
      661 cttctccatg aggtcgatca gcctggcctc gacgaatttc ttggactcgt cggagaagac
      721 gccggcggtg atgcagtagc ccttgccggc cttgaggtcc ttgatcctcg cgtggaagtc
      781 gcgtagcacc agctcgccta tcgtaccggt cgaacgtatc atcctgaaca gcaccacgtc
      841 cgaccagcgg ctcgtctcga cctcggccag gatgtcggcc cagtcgttct tctgcaccga
      901 gatatcggta atcttgacct tggccttggg gaagaacgtc agggttatct tgcggcacag
      961 cgtgatgaag tcgctcgtgg ccgacagcag gtacacctgc aggttccggt tggagttcag
     1021 ctccttgtac ttcgcgagct gggccggcgt atccttatag ttcgggtaga tctgctggat
     1081 ctcgttcagg agggaaacgg ccttggctat ctcctggccc ttcaggtagg cggccgccag
     1141 gcggtacttg atctcgacga ggacctcgac cttgatgtcc gggtgccgaa gccccagctc
     1201 gaagtccata atggccttct ggtactgccg ctggttcagg tggatagtgc cggcgaacag
     1261 cgccgccgac ggtccgaggg cggggtcggt gcgcaggtgc gtgaatagcc tgatcgcctg
     1321 ctcctcctgc cctatctcgt ggaagcactc gcccatggcg aacaggcttt ccttgtcatc
     1381 cggctgcagg tccaccgcgc gcttcaggct ggatagggcc tccttgaagt tcttgacctt
     1441 gaacagcgaa tgccccaagt agcgcagcgt catcgcgtgc tcggggttct gccgccccgc
     1501 cagccggagg tggaagatcg ccttctcgta ctgccgcttc tggaactcga ggtagccgag
     1561 gttgtagttc gtctcgaagt cgtcggcctt gaccgcgtgc gccagcacca ggcccttgta
     1621 ggactcgtcg aggcggccga gcttcatcgc gcagaggccg tagcgcttgt tgatttcgaa
     1681 ctcgtccaga tcgggattgc cggcggacac ctcgaccagg acctcgtagg ccttgaaggc
     1741 cttatcccag gcctgctcgc cgtagtacac ctcgccgagc gcctgtagcg catccgggtc
     1801 cttggggttc tgggccagcc tgcggttcgc ctctttcagg acctgcgcgt ggtccttggc
     1861 cttgatcctt ttgccaccct tggacttgcc tcccttgccc ttggacagga cggctccgcc
     1921 cacgatgacg accagggcga g
//
LOCUS       DAOOLN010000313         1937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_74742_length_1937_cov_3.210946, whole genome shotgun sequence.
ACCESSION   DAOOLN010000313 DAOOLN010000000
VERSION     DAOOLN010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74742_length_1937_cov_3.210946"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1289)
                     /locus_tag="PLW80_04810"
     CDS             complement(165..1289)
                     /locus_tag="PLW80_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB65858.1"
                     /translation="MPNRNRLHGEEGRAYWSMVLPAFSIYLLVMAFPIVLSIVLSVSN
                     YSGGKMFGGEPWGFVGLKSYARVFSDPLFWNALKNNFYIVLLSVFGQLPLGFIFAYII
                     YRRLVKWPGFWQGVLYVPNIISVIVVGLLWQTIFSPNGPIAEVMNDLARAGFQSRLEA
                     LFASVGGFNVDDGLVSKILDMAGPAALDWFSDPVPELKELILSYEGAPLSELIGDLCN
                     LFVPRWSPEFLSKPNIAMLPVLFVILWMYTGMYLILFLANMQKIDSQVIEAARIDGAS
                     EGQVMRYVILPALSGTIVNSAILAISGSLSSFSLIFAMTGGGPSRVTEILSIYMYNNA
                     FLGRPNLPLANAISLVMVVISFILIGLTKMAEKRYGGKEE"
     gene            complement(1390..>1937)
                     /locus_tag="PLW80_04815"
     CDS             complement(1390..>1937)
                     /locus_tag="PLW80_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPB65859.1"
                     /translation="AGMFASGKAAIYIDGDWRAGAYITDKASGKALISPAAQDKDFAL
                     MPFPAIPGEKNRGVVSAVAGTGWAVSASLPAGSAKEKAATRLIQYLYSNDVQAIRFNT
                     GAYVPTRKGVKADVEPLVAKVPVFYAENPATCYVIDGVLDPAVYNVINDGLIAIGLGT
                     KTAAQVAAETQAAFESWKASK"
BASE COUNT          380 a          582 c          639 g          336 t
ORIGIN      
        1 ctggctcacc tggaactccc gcgtcgactt gaaggaattc atgatcagcc agaagatcgg
       61 gtacagcgtc atcgccgtga agagcagcat catcgcatag gccgctgcct tggcggccgc
      121 cccgcctatc cggccgagcg cggtggtctt gatctcgtcg ctcatcattc ctccttgccg
      181 ccgtagcgct tctcggccat cttggtcagc cctatcagga tgaagctgat gacgaccatg
      241 accaacgaga tcgcgttggc cagcggcagg ttcggcctgc ccaggaacgc gttattgtac
      301 atgtaaatgg ataggatctc ggtaaccctg gacgggccgc cgccggtcat cgcgaatatg
      361 agcgagaagc tcgaaagcga tccggagatc gccaatatgg ccgagttgac gatggtgccg
      421 gacagggccg gcagaatcac gtagcgcatg acttgaccct cgctggcgcc gtcgatgcga
      481 gcagcctcta tcacctggga gtcgatcttc tgcatgttcg ccaggaagag gatgaggtac
      541 atgccggtat acatccacag tatgacgaac agcaccggta gcatggcgat gttcggcttg
      601 gacaggaact ccgggctcca gcgaggcacg aacaggttgc acagatcgcc gatgagctcg
      661 gatagcgggg ccccctcgta tgataagatc agctccttca gctcgggtac ggggtccgag
      721 aaccagtcga gcgcggccgg cccggccatg tccaggatct tcgataccag cccgtcgtcg
      781 acgttgaagc cgccgaccga ggcgaacagc gcctccagcc tcgactggaa gcccgccctg
      841 gccaggtcgt tcattacctc ggcgattggg ccgttcggcg agaagatggt ctgccagagg
      901 aggccgacca cgatgaccga gatgatgttc ggtacgtaca ggacgccctg ccagaagccg
      961 ggccatttga ccaggcgccg gtagatgata taggcgaaga tgaagccgag cggcagctgc
     1021 ccgaacaccg acagcagcac gatatagaaa ttgttcttca gcgcattcca gaacaacgga
     1081 tcggagaata ctctcgcgta cgatttgagg ccgacgaagc cccagggctc gccgccgaac
     1141 atcttcccgc cggaataatt ggatacggac agcacgatgg acagcacgat ggggaacgcc
     1201 atcaccagca ggtatatcga gaaggccggc aacaccatgg accagtaggc ccggccttcc
     1261 tcgccatgga gtcggtttct attcggcatg ggatcctctc atgcggcggc gccgcgcgcc
     1321 tcgggcgccg cgccaggtcg acgaaggcgg gcgcggcgcc ggaggcggcg tcgcgccctt
     1381 tcaagcgagt tacttcgagg ctttccagct ctcgaaggcg gcctgggttt cggcggccac
     1441 ctgggcggcg gtcttggtac cgagaccgat ggcgatgagg ccatcgttga tcacgttata
     1501 cacggcgggg tcgaggacgc cgtcgatcac gtagcaggta gccgggttct cggcgtagaa
     1561 gaccggaacc ttggcgacga gcggctcgac gtcggccttg acgcccttgc gggtcgggac
     1621 gtaggcgccg gtgttgaagc ggatagcctg gacgtcgttc gagtacaggt actggatcag
     1681 cctggtcgca gccttctcct tggcggagcc ggcggggagc gacgcggaaa cggcccagcc
     1741 ggtgccggcg accgcggaga cgacgccgcg gttcttctcg ccggggatag ccgggaacgg
     1801 catcagcgcg aagtccttat cctgggcggc cggggagatc agggccttgc cggaagcctt
     1861 atcggtgatg taggcgccgg ccctccagtc gccgtcgatg tagatggcgg ccttgccgga
     1921 cgcgaacata ccggcgg
//
LOCUS       DAOOLN010000314         1929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_75226_length_1929_cov_3.691569, whole genome shotgun sequence.
ACCESSION   DAOOLN010000314 DAOOLN010000000
VERSION     DAOOLN010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75226_length_1929_cov_3.691569"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PLW80_04820"
     CDS             complement(<1..662)
                     /locus_tag="PLW80_04820"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPB65860.1"
                     /translation="MIRTINFRLNESEYHVKVREEALALDWIRNEAGLKGTKEGCREG
                     DCGACLVLLGGSAPRRDGAPDGAVEWRSVTSCTLAMGELDGKHLITIEGLASAGLTPV
                     MEAMLDEGASQCGFCSPGFVLALTGFLVSGARVDPVEAMLAVEGNLCRCTGYGSIRRA
                     AAKLAADFADLPASLAERLTVLAGRRVLPAGLAALMAEPPVAAIPTDADSAGEAVVIE
                     GQT"
     gene            complement(877..>1929)
                     /locus_tag="PLW80_04825"
     CDS             complement(877..>1929)
                     /locus_tag="PLW80_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HPB65861.1"
                     /translation="YEKLGKGMWIMARMGSSARDLENLIPAMDGESVRRTMLCTDDRH
                     PNDIVSEGHIDAAVRALVSGGVPPIDALRMACHNPATYYGLRRSGAIAPGYAADLVAF
                     SSLEDFRAELVLKAGAVVAEGGVPTAAYERLGGSAVVPPLRDSVNIKWLEEKDFRIPD
                     EGGDARVIESTPGSIITGASFERPTVRDGLCVSDIERDIVKIFVIERHTGSGNIGKGF
                     IRGLGLKRGAVGSTVSHDSHNMIIAGVDDASIFKAARRLNAMKGGYVITDGDEVLAEL
                     ALPVAGLMSDRPAVEVIEQLASFEDFFRMEGMPGASPLMTLSFMALPVIPALKITDKG
                     LVDVNSFEQVPLFRMP"
BASE COUNT          330 a          615 c          641 g          343 t
ORIGIN      
        1 gtctggcctt cgatgaccac ggcctcgccg gcgctatccg catcggtcgg gatagccgct
       61 acgggcggtt cagccatgag cgcggcgagg cccgccggca ggacgcgacg tccggcgagc
      121 accgtcagcc gctcggccaa gctggcgggc aggtcggcga aatcggcggc cagctttgcg
      181 gcggctcgcc ggatggagcc gtagccggtg catcggcaga ggttaccctc gacggcgagc
      241 atggcctcga ccgggtcgac acgggcgccc gagaccagga agccggtcag cgctagcacg
      301 aagcccggag aacagaagcc gcactggctg gcgccctcgt cgagcatcgc ctccatgacc
      361 ggcgtcaagc cggccgaggc caggccttcg atcgtgatca ggtgcttgcc gtcgagctct
      421 cccatcgcga gggtgcatga cgtaacggaa cgccactcga cggcgccatc gggcgcgccg
      481 tctcgcctgg gcgccgaacc gcccagtagg acgaggcaag cgccgcaatc gccttcgcgg
      541 cagccttcct tggtgccctt gagcccggcc tcgttgcgta tccaatcgag cgccagcgcc
      601 tcttccctga ctttgacgtg atattcgctc tcgttgagcc tgaagttgat ggttcgtatc
      661 atgcgtggtc cttccgtatg cgggatgtaa ggagtccgcc tgaagtcgcg ggtaaccccg
      721 cgatcgcctg ggccccgaga cgtgtacgag cattatggtg agaagctagt ggtgtataat
      781 aactgtagtc caaggacgac ccgggagcaa tggcaaggca tcgttcctga acgcgggtgg
      841 agtaccggag gcgccgccgg attcggccgg atgccgtcaa ggcattctaa aaagtggtac
      901 ctgttcgaac gagttaacgt cgacgagccc cttgtcggtt atcttgaggg ccgggataac
      961 cggtagggcc atgaaagaga gggtcatgag gggtgaggct cccggcatgc cctccatcct
     1021 gaagaaatct tcgaatgagg cgagttgttc tatgacctcg accgcaggac ggtcgctcat
     1081 gagtccggcg accggtaggg ccagctcggc cagcacctcg tctccgtcgg tgatgacgta
     1141 gccgcccttc atcgcgttga gcctgcgcgc cgccttgaag atcgaggcgt catcgacgcc
     1201 ggcaatgatc atgttgtgcg agtcgtggct gaccgtcgat ccaacggctc cgcgcttgag
     1261 gccgagcccc ctgatgaagc ccttgcctat gttcccggag ccggtatgac gctcgatcac
     1321 gaatatcttg acgatgtcgc gctcgatgtc cgatacgcat aggccgtcgc gcacggttgg
     1381 gcgctcgaac gacgcgccgg ttatgatcga tccgggcgtc gactcgatga cgcgggcatc
     1441 gccgccctcg tccggaatcc taaaatcctt ttcctcgagc cacttgatat tgaccgagtc
     1501 gcgcaggggc gggactaccg cggagccgcc gagccgctcg taggctgccg tcggaacgcc
     1561 gccctcggct acgaccgcgc cggccttgag cacgagctcg gccctgaaat cctcgaggct
     1621 ggagaacgcg acgaggtcgg cggcgtagcc cggggctatg gcgccggagc gcctgaggcc
     1681 gtaataggta gcggggttgt ggcaggccat acgcaacgcg tcgatcggcg gcacgccgcc
     1741 cgagaccagc gcccgtacgg cggcgtcgat atgcccctcg gacactatgt cgttcgggtg
     1801 cctgtcgtcg gtgcagagca tcgtgcgccg tacgctctct ccgtccatcg cggggatcag
     1861 gttctcgagg tcgcgggcgg acgagcccat gcgggccatg atccacatgc ccttcccaag
     1921 cttctcgta
//
LOCUS       DAOOLN010000315         1928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_75309_length_1928_cov_2.978110, whole genome shotgun sequence.
ACCESSION   DAOOLN010000315 DAOOLN010000000
VERSION     DAOOLN010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75309_length_1928_cov_2.978110"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /gene="rlmN"
                     /locus_tag="PLW80_04830"
     CDS             complement(<1..940)
                     /gene="rlmN"
                     /locus_tag="PLW80_04830"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPB65862.1"
                     /translation="MLPAEIASALPLSPAFRGKQVFKWIAKGAAGFGDMTDLPAADRE
                     RLSSTPLYSSRVAERLEADDGSVKLKIALEDGAAVEAVLLTDADGRRTACLSTQVGCR
                     MACAFCKTGTLGLLRNLGPDEIVEQFVHLSALRGKPSNIVFMGMGEPLDNLASLRKAI
                     VILMHPEGFGVSKRKITISTCGIVPGIVDLADNGPHVRLAVSLTAAEDALRSSLMPVN
                     RSWNLAALKEALLYYQAKTGDRVTLEAAIMGGENSGVDSAAAMAAWIRPLNAQVNVIP
                     WNPVPGLPHREPARGEAGAFEAELERRGVNAVRRARR"
     gene            complement(988..>1928)
                     /locus_tag="PLW80_04835"
     CDS             complement(988..>1928)
                     /locus_tag="PLW80_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB65863.1"
                     /translation="DAARKRSSHRDAVDYYKKCLIYHPGNNYALFGLADCYKALNQYQ
                     KAIEIWEQYLLHDNKNITVLTRIADAYRKVRDFRKSKAIYLRVLEMEMDNPYALIGLG
                     HLHYDFKEYRDALHYWQRMVDAQGDMVDIRVLTSIGNCYRKLKQFDQGVPYFEKALVK
                     EPDNFYALFGLADCFRGTGEPQRSLDYWNRILDKDPRNKVILTRAGDAYRSMDRFEEA
                     ADYYERALNIEFDVYAVLGLATISRLQGKVESAIESLKRLVQNDAKNYRLHLELAQCY
                     LAVGDRRKAEETLQDFMRLGIRNPHIQDLMAKIQNS"
BASE COUNT          324 a          592 c          631 g          381 t
ORIGIN      
        1 cgcgccgagc cctgcgcacc gcgttgaccc ccctgcgttc cagctcggcc tcgaaggcgc
       61 cggcctcgcc cctggccggc tcgcgatggg gcaggccggg caccgggttc cacggtatca
      121 cgttgacctg ggcgttgagc gggcgtatcc acgcggccat ggccgcggcg ctgtcgactc
      181 ctgagttctc gccgcccatg atggccgcct cgagcgtcac gcgatcgccg gtcttggcct
      241 gataatagag cagggcttcc ttgagcgcgg cgaggttcca cgagcgattg accggcatca
      301 gcgagctacg cagagcgtct tctgccgcgg tgagcgatac cgccaggcgt acgtgcgggc
      361 cgttgtcagc gaggtcgacg atgcccggca cgatgccgca ggtcgagatg gttatcttgc
      421 gcttggatac gccgaagcct tccgggtgca tcaggatgac gatggccttc ctgagcgacg
      481 cgaggttatc gagcggctcg cccatgccca tgaacacgat gttcgacggc ttgcctcgca
      541 gggccgacag gtgtacgaac tgctcgacaa tctcgtcggg accgagattg cgcaataggc
      601 cgagagtacc agtcttgcag aacgcgcagg ccatgcggca tccgacctgg gtggaaaggc
      661 acgccgtgcg gcggccgtcg gcgtcggtca gcaggacagc ctcgaccgcg gctccgtctt
      721 ccagcgcgat cttgagcttg accgatccgt cgtccgcctc cagcctttcg gcgacgcgcg
      781 acgagtagag cggcgtcgac gaaagacgct ccctgtcggc ggctggaagg tcggtcatgt
      841 cgccgaaacc ggccgcgccc ttggctatcc acttgaatac ctgcttcccg cggaaggccg
      901 gcgacagcgg cagggccgag gctatctcgg cgggaagcaa gccggcgacg ccgacccgcc
      961 gggaccgggc atttccgggc tcggcgctca cgagttctgg atcttggcca tcagatcctg
     1021 gatatgcggg ttgcggatac cgaggcgcat gaagtcctgt agcgtctcct cggccttgcg
     1081 cctatcgccg acggcgaggt agcactgggc cagctcgagg tgtagacgat agttcttggc
     1141 gtcgttctgg accaggcgct tgagcgactc gatagccgac tcgaccttgc cctgaagcct
     1201 cgagatggta gccaggccga gcaccgcgta cacgtcgaat tcgatgttca aggcgcgctc
     1261 gtagtaatcg gccgcctcct cgaagcggtc catgctccta taggcgtctc cggccctcgt
     1321 caggatgacc ttgttgcgtg gatccttgtc caatattcgg ttccagtagt cgagcgaacg
     1381 ctggggctcg ccggtgcccc ggaagcaatc ggccaggccg aacaacgcgt agaaattgtc
     1441 aggctccttg accagagcct tttcgaagta cgggacgccc tgatcgaact gcttgagctt
     1501 gcggtagcag ttgcctatgg aggtcagcac cctgatgtcg accatgtcgc cctgggcgtc
     1561 gaccatgcgc tgccagtagt ggagcgcgtc ccggtattcc ttgaaatcgt agtgaaggtg
     1621 gccgagaccg ataagggcgt agggattgtc catctccatc tcgagcacgc gcaggtagat
     1681 ggcctttgac ttcctgaaat cgcggacctt gcggtaggcg tcggctatcc gcgtcagtac
     1741 cgttatgttc ttattgtcgt gaaggaggta ctgctcccag atttcgatgg ccttttggta
     1801 ctggttgagc gccttatagc agtcggccag gccgaagagg gcgtagttgt tgcccgggtg
     1861 gtagatcaga catttcttgt aatagtcgac ggcatcgcgg tgcgacgagc gcttcctggc
     1921 cgcgtcgc
//
LOCUS       DAOOLN010000316         1927 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_75367_length_1927_cov_3.247329, whole genome shotgun sequence.
ACCESSION   DAOOLN010000316 DAOOLN010000000
VERSION     DAOOLN010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75367_length_1927_cov_3.247329"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..1475
                     /gene="gcvPB"
                     /locus_tag="PLW80_04840"
     CDS             42..1475
                     /gene="gcvPB"
                     /locus_tag="PLW80_04840"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HPB65864.1"
                     /translation="MNLKPEPLAFELSSPGRVGVALPKGDVPAAPVPETLLRDELKLP
                     ELDELTVVRHFTRLSQKNYSIDTEFYPLGSCTMKYNPKANEVAAALPGWKGSHPLASA
                     RCSQGSIELIWKLQESLKAIGGFEAVSLQPAAGAHGELAGVFMIRAYHLARGDAKRVK
                     MLIPDSAHGTNPASCTMAGLTTVTIPSDAEGGVDMAALESACDDTVAGIMITNPSTLG
                     LFERNIEKIARIVHAAGGLVYGDGANMNALTGVLRPADVGIDVMHFNLHKTFSTPHGG
                     GGPGVGMVGAGKALAAYLPGPIAVRDGDGYAMAAPSKTIGRMKAFYGNFGVLVRAYTY
                     IRMLGGDGLRRVAESAVLNANYLKARLEKAYPVKYPRRCMHEFVAMGNFAEGVHTLDI
                     AKRMIDYGFHPPTIYFPLIVPEALMIEPTETESKETLDGFVEVMLKIAEEAKSSPELL
                     REAPHATPVGRLDEVAAARCPVLCYRG"
     gene            1558..>1927
                     /locus_tag="PLW80_04845"
     CDS             1558..>1927
                     /locus_tag="PLW80_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65865.1"
                     /translation="MIDVFAQQCLSRLSIVAALVFAYCAWMVYSLNREAKPNRIAVVF
                     NVLFAAWAAAASFWYGAYDPSEALALYRAFSWTWSLFPPLILHFSLSVTGNPVLDGRF
                     RRPFQVALYAPGALLCFLTPA"
BASE COUNT          322 a          672 c          606 g          327 t
ORIGIN      
        1 cagatcgagc tcctggcgag gtcgctcgag gaggcctccg tatgaacctg aaacccgaac
       61 ctctggcctt cgagctgtcg agccccgggc gcgtcggcgt ggccctgccc aagggcgacg
      121 tgcctgcagc gccggtgccc gagacgctac tccgcgatga gctcaagctg ccggagctcg
      181 acgagctgac ggtcgtcagg cacttcacca ggctgtcgca gaagaactac tcgatcgaca
      241 ccgagttcta cccgctcggc agctgcacga tgaagtacaa cccgaaggcc aacgaggtcg
      301 ccgccgcgct accgggctgg aagggctcgc atccgctggc ctccgctcgc tgcagccagg
      361 gctccatcga gctcatctgg aagctccagg agagcctcaa ggccatcggc ggcttcgagg
      421 ccgtcagcct gcaaccggcc gctggcgccc acggcgagct ggccggggtc ttcatgatca
      481 gggcctacca cctggcccgg ggcgacgcca agcgcgtgaa gatgctgata cccgacagcg
      541 cccacggcac caacccggcg tcgtgcacga tggcgggcct gaccacggtc accatcccga
      601 gcgacgccga gggcggcgtc gacatggcgg ccctcgagtc cgcctgcgac gacacggtcg
      661 ccggcatcat gatcaccaat ccgagcaccc tcggactctt cgagcgcaac atcgagaaga
      721 tagccaggat agtccatgcg gccggcggtc tcgtctacgg cgacggcgcc aacatgaacg
      781 cgctgaccgg cgtgctcaga ccggccgacg tcggcatcga cgtgatgcac ttcaacctgc
      841 acaagacctt ctccacgcct catggcggcg gcggccccgg cgtcggcatg gtcggggccg
      901 gaaaggccct cgccgcgtac ctgcccgggc cgatagccgt ccgcgatggc gacggctacg
      961 cgatggcggc tccatccaag accataggcc gcatgaaggc tttctacggc aacttcggcg
     1021 tgttggtccg agcctatacc tacatcagga tgctcggcgg cgatggcctg cggcgcgtgg
     1081 ccgagtctgc ggtgctgaac gcgaactacc tcaaggccag gctcgagaag gcctacccgg
     1141 tcaagtaccc acgccgctgc atgcacgagt tcgtggcgat gggcaacttc gccgagggcg
     1201 tccataccct ggacatcgcc aagcgtatga tagactacgg cttccatccg ccgacaatct
     1261 atttcccgct catcgtcccg gaggccctga tgatagagcc gaccgagacc gagagcaagg
     1321 aaacgctcga cggcttcgtc gaggtgatgc tcaagatagc cgaggaggcg aagagctctc
     1381 cggagctgct ccgcgaggcg ccccatgcta cgccggtggg ccgactcgac gaggtggccg
     1441 cggccaggtg tccggtgctc tgctaccggg gctgagcgag gtatcgtcgt atcgtcgggc
     1501 ggggaggcca agcctccccg ccttttcttt cgtcccggcg tagtatagaa tccgcccatg
     1561 atcgacgttt tcgcgcagca gtgcttgtcc aggctatcga tcgtcgcggc gctcgtgttc
     1621 gcgtattgcg cctggatggt gtactcgctg aaccgggaag ctaagccgaa ccggatcgcg
     1681 gtcgtcttca atgtcctgtt cgcggcctgg gccgccgccg cctcgttctg gtacggcgcc
     1741 tacgacccgt ccgaagcgtt ggcgctatac cgagccttct cgtggacctg gtcgctcttc
     1801 ccgccgctca tcctgcattt ctccctcagc gttaccggca atcccgttct cgacggccgc
     1861 ttccgccggc cgttccaggt cgccctctac gcgccgggcg cgctcctgtg cttcctgacc
     1921 ccggcct
//
LOCUS       DAOOLN010000317         1927 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_75394_length_1927_cov_1.995726, whole genome shotgun sequence.
ACCESSION   DAOOLN010000317 DAOOLN010000000
VERSION     DAOOLN010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75394_length_1927_cov_1.995726"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PLW80_04850"
     CDS             complement(<1..818)
                     /locus_tag="PLW80_04850"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HPB65866.1"
                     /translation="MIDTELDFAWILREHGERDLAAARDMFPIEVARKSRKFHRQIPG
                     YRTSPLLALPHLSAMFGVGGIWVKDEASRMELNSFKVLGGSFALYRFIQERLGIPDDK
                     MSYEYLLSKEVKDRLGSITFASATDGNHDRGIAWAAQKLGHPCVIYVHAETSRRRIDA
                     IRGYGATVKIIDGNYDDAVRQIAVDSAKYGWEIISDTSWDGYTAVPTWIMQGYTTMLA
                     EAQEQFSAQGVVKPTHIFVQAGVGALAAAVTGYYHNLFGPDAPVCVVVEPDNAA"
     gene            914..1450
                     /locus_tag="PLW80_04855"
     CDS             914..1450
                     /locus_tag="PLW80_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPB65867.1"
                     /translation="MNETKEKSQWRGRIAAVTTFKYLSPSELDFLIDKAAVVTCRPEE
                     TIVREDEVSQYFFAILDGTVNVTVDEASLDGSLRSVYICTLGPGDVFGEAGIFIKVKR
                     TATVSAADEVTLLRLHRDDMSAFIRSKPAGGNKLLLVIIYSLLRKLRAANQELAYERK
                     ADMDQADVDSLMADMLGD"
     gene            complement(1457..>1927)
                     /locus_tag="PLW80_04860"
     CDS             complement(1457..>1927)
                     /locus_tag="PLW80_04860"
                     /inference="COORDINATES: protein motif:HMM:NF026042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65868.1"
                     /translation="WFAGEWTTMGTGTPAVTVSGIGAADRVLRERGLLEFRNVPRGNS
                     VVRLLPTGTPGNVPASAAGVEASRCRWCERPPCALACPAGIDVPGALRRLECGNEAGA
                     ARRVAESDRGRPGREPGRVSCVGCAAPCLEVCIRRDVDGRPVAIAELLTGLERT"
BASE COUNT          310 a          645 c          649 g          323 t
ORIGIN      
        1 caggccgcgt tgtccggctc gacgacgacg cagaccggcg cgtcgggccc gaataggttg
       61 tggtagtagc cggtgaccgc cgcggctagg gcgccgacgc cggcctgcac gaagatgtgc
      121 gtcggcttga cgacgccctg ggcgctgaac tgctcctggg cctcggcgag catggtggta
      181 tagccctgca tgatccaggt cggcacggcc gtgtagccgt cccaactagt gtcgctgatg
      241 atctcccagc cgtacttggc gctgtcgacc gctatctgcc tgaccgcgtc gtcgtagttg
      301 ccgtcgatga tcttgacggt ggcgccgtag ccccggatcg cgtcgatcct gcgccggctc
      361 gtctcggcgt gcacgtagat aacgcagggg tggccgagct tctgggcggc ccaggcgatg
      421 ccccggtcgt ggttgccgtc ggtggcgctc gcgaaggtga tggagccgag gcggtccttg
      481 acctccttcg acaggaggta ctcgtacgac attttatcgt cggggatgcc gaggcgttcc
      541 tggatgaagc gatagagcgc gaacgagccg ccgagcacct tgaagctgtt gagctccatg
      601 cgcgacgcct cgtccttgac ccagatgccg cctaccccga acatcgcgga caggtggggc
      661 agcgccagga gcgggctcgt cctgtacccg ggtatctggc gatggaattt cctgctcttc
      721 ctggccacct cgatcgggaa catatcgcgc gcggccgcga ggtcccgctc gccgtgctcg
      781 cggagtatcc aggcgaaatc tagctctgtg tcgatcattg tacgctcctg atgtcctatt
      841 gtcgaaatat ccacggtctt gtcaaggacg gcgcgggatt gcgctaggat gcgccgtggc
      901 ttagaatccg ggtatgaacg aaactaagga aaagtctcag tggcgcgggc gcatagccgc
      961 cgtaacgacc ttcaagtacc tgtccccgag cgagctcgac ttcctgatcg ataaggccgc
     1021 cgtggtgacc tgccgccccg aggagaccat cgtccgggag gacgaggtca gccaatactt
     1081 cttcgccatc ctcgacggca ccgtcaacgt gacggtggac gaggcctctc tggacggttc
     1141 gctccgtagc gtatacatct gcacgctcgg ccccggcgac gtcttcggag aggccggcat
     1201 cttcatcaag gtcaagcgga ccgcgacagt cagcgccgcc gacgaggtga cgctcctgcg
     1261 cctgcaccga gacgacatga gcgccttcat ccggagcaag ccggccggcg ggaacaagct
     1321 cctgctcgtg atcatctaca gcctgctgcg caagctgcgc gcggccaatc aggagctggc
     1381 ctacgagcgc aaggccgaca tggaccaggc cgacgtcgac agcctgatgg ccgacatgct
     1441 cggcgactga ggcgcgctag gtccgctcga ggccggtgag cagctcggcg atagctaccg
     1501 ggcggccgtc cacgtcgcgg cggatgcata cctcgaggca aggcgcggcg cagccgacgc
     1561 acgagacccg gccaggctcg cggcctggcc gcccgcggtc gctctccgcg acgcgcctcg
     1621 cggcgccggc ttcgttgccg cattccaggc gccgcagggc gcccggtacg tcgatgccgg
     1681 cggggcaggc tagcgcgcag ggcggccgct cgcaccagcg gcatcgcgac gcctcgacgc
     1741 cggcggcgct cgccggcacg ttgcccggcg tcccggtcgg caacaggcga acgaccgaat
     1801 tcccgcgcgg cacgttgcgg aattccagaa ggccgcgttc cctgagcacg cggtcggcgg
     1861 cgcctatgcc cgagacggtt accgccggcg taccggtgcc catcgtcgtc cattcgccgg
     1921 cgaacca
//
LOCUS       DAOOLN010000318         1926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_75422_length_1926_cov_3.153394, whole genome shotgun sequence.
ACCESSION   DAOOLN010000318 DAOOLN010000000
VERSION     DAOOLN010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75422_length_1926_cov_3.153394"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PLW80_04865"
     CDS             complement(<1..318)
                     /locus_tag="PLW80_04865"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPB65869.1"
                     /translation="MDEERRNEKRFRLSRMVAYDMEREQYIRAHGFEISKGGMAFLST
                     EYVEPDCVVWLSFSIPEEDGSWRQLEAEGRVREVSSIPSGTRFGVAFTRMADEDRVAL
                     DAFL"
     gene            complement(412..>1926)
                     /locus_tag="PLW80_04870"
     CDS             complement(412..>1926)
                     /locus_tag="PLW80_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPB65870.1"
                     /translation="MDEADGHGISVFSENLKNLLLSAPLKGTRVLGVDPGIRTGTKCA
                     AIDETGKYLGYFVIYQEQKPDEARKALAKAIRDYRIELVAVGNGTASHEVQKLVADAL
                     GDLGGEAKYAVVDEDGASVYSASDVAREEFPELDLTIRGAISIGRRIQDPLAELVKID
                     PKSIGVGLYQHDVNQKRLSETLDEVVESVVNRVGVNINTASHMLLKYVSGINSSLAKK
                     IVKYRDENGTITSRDQLKGVSGMGDKSFEQCAGFLKIPESPNPLDNTWVHPENYALAS
                     ELLEGVRAGKDPDKAQRDALKAKYGVGDTTIDDIVAELKKPNRDPRDDYPKPVLQQGV
                     LAFEDLREGMKVTGKVKNVVDFGAFVDIGIKESALIHVSELSDQFVKDPMDVLKVGDV
                     KEFRIISLDVARKRIGLSLKSERASGGQSFGNQRPSGYGGAQPGAAQSPDSGTRRVVV
                     AKKPAGGQSSPVGGQRSEKNPYSVGGRPGGRPSQDRDDDGTSYNPFAELLKNRK"
BASE COUNT          297 a          620 c          602 g          407 t
ORIGIN      
        1 gaggaaggcg tcgagcgcga ccctatcctc gtcggccatt ctggtaaagg ctacgccgaa
       61 acgagtccca ctggggatgc tgctaacctc gcggacgcgc ccttctgcct ccagctgccg
      121 ccatgacccg tcctcctcgg gaatggagaa cgatagccat acgacgcaat ccggctcgac
      181 gtactcggtc gacagaaagg ccataccccc cttcgatatc tcgaacccgt gggcgcggat
      241 atactgctcc cgctccatat cgtaggcgac catccgtgag aggcgaaagc gcttttcatt
      301 ccgtcgctct tcatccatgc cataactata ctaccgttgt cggacgaacg ctataacaaa
      361 cacgggcccg gccgaggcac gttccgcgac tgacccctcg tcctcgagga gctatttcct
      421 gttcttcagg agctccgcga acggattgta gctcgtgccg tcgtcgtccc ggtcctgtga
      481 cggacgacct ccgggtctgc cgcctacgga ataggggttt ttctccgacc tctgtccccc
      541 gacgggcgag ctctgtcccc cggccggttt cttagcgacg acgacgcgcc ttgttccgga
      601 atcggggctc tgcgccgcgc ccggctgcgc gccgccgtaa cccgatggcc gctggtttcc
      661 gaagctctgt cccccggagg ctcgttcgct cttgagcgac aggccgatgc gcttgcgggc
      721 gacgtcgagg ctgatgatgc ggaattcctt gacgtcaccg accttgagca cgtccatagg
      781 atccttgacg aactgatcgg acagctccga tacgtggatc agggcgcttt ccttgatgcc
      841 gatgtcgacg aaggcgccga agtcgacgac gttcttgacc ttgccggtca ccttcatgcc
      901 ttcgcggagg tcttcgaagg ccaggacgcc ctgctggagc accggcttcg gatagtcgtc
      961 tcgcgggtcg cgattcggtt tcttgagctc ggcgacgata tcgtcgatcg tcgtgtcgcc
     1021 gacgccgtac ttggccttga gcgcgtcccg ctgggccttg tccgggtcct tgcccgcgcg
     1081 cacgccctcg agcagctcgg aggcgagggc gtagttctcg gggtgcaccc aggtattgtc
     1141 gagcgggttc gggctctcgg ggatcttgag gaagccggcg cactgctcaa agctcttgtc
     1201 gcccatgcct gagacgccct tgagctggtc ccggctcgtg atcgtgccgt tttcgtcgcg
     1261 gtacttgact atcttcttgg cgagggacga gttgatgccc gacacgtact tgaggagcat
     1321 atggctggcg gtattgatgt taacgccgac ccggttgacc acggactcga ccacctcgtc
     1381 gagggtctcg gaaaggcgct tctggttgac gtcgtgctgg tagaggccga cgccgatgct
     1441 cttggggtcg atcttgacga gctcggcgag cgggtcctgg atgcggcggc ctatcgatat
     1501 ggcgccacgt atggtcaggt cgagctcggg gaactcctcg cgagccacgt cgctcgcgga
     1561 atagaccgaa gcgccgtcct catcgacgac cgcgtacttg gcctcgccgc ccaggtcgcc
     1621 gagggcgtcg gccacgagct tctgcacctc gtggctcgcg gtgccgttgc cgacggccac
     1681 gagctctatc ctatagtcgc ggatggcttt ggcgagcgcc ttacgcgcct cgtcgggttt
     1741 ctgctcctgg tagatgacga agtagcccag gtacttgccc gtctcgtcga tcgctgcgca
     1801 cttggtaccg gtgcgtatgc cagggtcgac gccgagcacg cgcgtgccct tgagcggcgc
     1861 ggacaggagc aggttcttga ggttctcgct gaacaccgag atgccgtgcc cgtcggcctc
     1921 gtccat
//
LOCUS       DAOOLN010000319         1925 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_75484_length_1925_cov_2.633690, whole genome shotgun sequence.
ACCESSION   DAOOLN010000319 DAOOLN010000000
VERSION     DAOOLN010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75484_length_1925_cov_2.633690"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1575
                     /locus_tag="PLW80_04875"
     CDS             <1..1575
                     /locus_tag="PLW80_04875"
                     /inference="COORDINATES: protein motif:HMM:NF036553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5312 family protein"
                     /protein_id="HPB65871.1"
                     /translation="PRMPTNNDPASRDDGDSSAGFLQKLLGLFGMSSPDADKKRLLKN
                     LAKDLSRSRYKFYKPKTGEALPGLARFFYETYKVIAPAQLMLTNASQSGVLKSFVIES
                     FLTPEQRQMSECMTDAYIQEKAKTMNVKDLQEHVKQVMLSFFAAFDAEKTAQIDGAYS
                     TLLSFINFINFDFFFLLKKFDSNVAERNFSYNPKFDAISADYVADDLHDFLEVFLPLN
                     LDADWKRIFTALKEYRSMDVIQVDTWVKLVPALRDVRESGVLESVVMHAKKDPTLTVA
                     PRAASERIVESFIEKSKNQTAMTLQKIAQERRNSKIDELAKAVFGTTIVLRMKNYTDK
                     SNVAFAKKMLGGYTQTQALNFLKAYLIDYFKKDIREIVDVLLIRGQWSTNVQSQQLSD
                     GYHALLEVSEGILQFDDSLADDGELGNKLRNVLGKADRDKDALKYVRQILKDVNEKAL
                     GLVNKAALNLITVGRHLKALIDDLGKPHHELILNWKEVEGAATVPMKEAMTESYKKIY
                     YMVQLLQYYVKEESAH"
     gene            complement(1619..>1925)
                     /locus_tag="PLW80_04880"
     CDS             complement(1619..>1925)
                     /locus_tag="PLW80_04880"
                     /inference="COORDINATES: protein motif:HMM:NF039579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65872.1"
                     /translation="LNKIDRLADRSVPSLPFEGVSVLTSAVTGEGLPALVSSIEKALT
                     ADALELTLRVPPEDYAVVPLLYREATVLSEDHDGEATIVRCRVPERLGSRVEKYRVD"
BASE COUNT          413 a          645 c          564 g          303 t
ORIGIN      
        1 ccgcgtatgc ccacgaacaa cgacccggcc agccgggatg acggcgactc gtccgccgga
       61 ttcctccaga agctgctagg gctcttcggc atgagcagcc cggacgccga caagaagcgc
      121 ctgctcaaga acctcgccaa ggacctgtcg cgctcccgct acaagttcta caagcccaag
      181 acgggcgaag ccctgcccgg gctcgcccgc ttcttctacg agacctacaa ggtcatcgcg
      241 ccggcgcagc tgatgctgac gaacgcgtcg cagagcggcg tgctcaagtc cttcgtgatc
      301 gagagcttcc tgacgccgga gcagcgccag atgtcggagt gcatgaccga cgcgtacatc
      361 caggagaagg ccaagacgat gaacgtcaag gacctccagg agcacgtcaa gcaggtgatg
      421 ctatcgttct tcgcggcgtt cgacgcggag aagacggccc agatagacgg cgcctactcc
      481 acgctattgt ccttcatcaa cttcatcaac ttcgacttct tcttcctgct caagaaattc
      541 gactcgaacg tggccgagcg caacttctcg tacaacccca agttcgacgc catctccgcg
      601 gactacgtgg ccgacgacct gcacgacttc ctcgaggtct tcctgccgct caacctcgac
      661 gccgactgga agcgcatctt caccgcgctc aaggaatacc gctcgatgga cgtgatccag
      721 gtcgacacct gggtcaagct cgtccccgcc ctgcgcgacg tgcgggagtc gggcgtgctc
      781 gagagcgtcg tgatgcacgc caagaaggac ccgacgctga ccgtggcccc ccgggccgcg
      841 tcggagcgca tcgtcgagag cttcatcgag aaatccaaga accagaccgc gatgaccctg
      901 cagaagatcg cccaggaacg gcgcaactcc aagatcgacg agctggccaa ggcggtcttc
      961 ggtacgacca tcgtgctgcg catgaagaac tataccgata agtcgaacgt cgccttcgcc
     1021 aagaagatgc tcggcggcta cacccagacc caagcgctca acttcctcaa ggcctacctg
     1081 atcgactact tcaagaagga catccgcgag atcgtcgacg tgttgctgat acgcggccag
     1141 tggtcgacca acgtccagtc ccagcagctg tcggacggct accacgccct gctcgaggta
     1201 tcggagggca tactccagtt cgacgacagc ctggccgacg acggcgagct cggcaacaag
     1261 ctacgcaacg tgctgggcaa ggccgaccgc gacaaggacg cgctcaagta cgtcaggcag
     1321 atactcaagg acgtcaacga gaaggccctg ggcctcgtca acaaggccgc gctcaacctg
     1381 atcaccgtcg ggcgccacct caaggcgctg atcgacgacc tcggcaagcc ccaccacgag
     1441 ctgatcctga actggaaaga ggtggagggc gccgcgaccg tcccgatgaa ggaagcgatg
     1501 accgagtcgt ataagaagat ctactatatg gtccagctgc ttcagtacta cgtcaaagaa
     1561 gaatcggctc attagagccg tggctcatga gagccgtggc gccgcctaag cggcgcgatc
     1621 agtcgaccct gtacttctcc acccgagagc cgaggcgctc gggcaccctg caacgcacga
     1681 tcgtcgcctc gccgtcgtgg tcctcggaca gcaccgtcgc ctcgcggtac aggagcggca
     1741 ccacggcgta gtcctcgggc ggcacccgca gcgtcagttc gagcgcgtcg gcggtcagcg
     1801 ccttctcgat ggaggagacc agggcgggta gcccctcccc ggtcacggcc gaggtcagca
     1861 ccgagacgcc ctcgaacggc aggctcggca cggaacggtc cgccagccgg tctatcttgt
     1921 tgagg
//
LOCUS       DAOOLN010000320         1919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_75800_length_1919_cov_3.950644, whole genome shotgun sequence.
ACCESSION   DAOOLN010000320 DAOOLN010000000
VERSION     DAOOLN010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75800_length_1919_cov_3.950644"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="PLW80_04885"
     CDS             <1..1206
                     /locus_tag="PLW80_04885"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPB65873.1"
                     /translation="APTMQGSHKVHGAPLGADEIAKAKAAMGLPADAEFWVSPEAKAF
                     FAARRGELSAGRTAWQAEFTAWKTENPAKAAELEAMLAGKPLTAPAWPEAKVGDSVAT
                     RNASGAALKAAFAAWPGLVGGSADLTGPNVTALPCAPYSKADRSGRMIHYGIREHAMA
                     AISNGLALHGGFKPFCATFLVFADYLRPSLRLAALMGLPVVYVMTHDSVFVGEDGPTH
                     QPIEHLASLRSIPNVLVLRPADAEETAEAWAWAMARTTGPTVIALTRQNLPVFEKADP
                     MWRDTFRAGAYVARTTRGEPDVVVLASGSEVELALKAAELASSKSVRVVSVPSLETFL
                     AQPDALRDTIAPPEARIVACEAGRSMPWNGVADGFLGIERFGESGPGAKVAAHLGLTL
                     EALAALINA"
     gene            complement(1315..1596)
                     /locus_tag="PLW80_04890"
     CDS             complement(1315..1596)
                     /locus_tag="PLW80_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65874.1"
                     /translation="METRNGLINFTLFIFIFVFSFVFSLDALAAPNTFYGVLALLGFV
                     VCLAGSLFNGLLSKRDGEALAIWYFSYAVVVGIVTVWYMTRCGTAFGWW"
     gene            1704..>1919
                     /locus_tag="PLW80_04895"
     CDS             1704..>1919
                     /locus_tag="PLW80_04895"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HPB65875.1"
                     /translation="MKKACVILANGCEEVEAITPIDYLRRAGVEVVAAGLDGREAVGA
                     HGLIIGADAVLDDIDDEEFDCIVVPGGG"
BASE COUNT          329 a          648 c          662 g          280 t
ORIGIN      
        1 gcgccgacca tgcagggctc gcacaaggtg catggagccc ctctcggcgc cgacgagata
       61 gccaaggcca aggccgcgat gggcctgccg gccgacgccg aattctgggt gtcacccgag
      121 gccaaggcct tcttcgcggc caggcgcggt gagctctcgg ccgggcgcac cgcatggcag
      181 gccgaattta cggcgtggaa gacagagaac ccggccaagg cggccgagct cgaggcgatg
      241 ctcgcgggca agccgctcac cgcgccggca tggcccgagg caaaggtcgg cgactcggtt
      301 gccacgcgca acgcctctgg cgccgctctc aaggcggcct tcgccgcgtg gccgggcctc
      361 gtcggcggct cggccgacct gaccggcccg aacgtcaccg ccctgccctg cgcgccgtac
      421 tcgaaggcgg accgctcagg caggatgatc cactacggca tccgcgagca cgcgatggcg
      481 gctatttcaa acggcctggc cctgcacggc ggcttcaagc cgttctgcgc caccttcctc
      541 gtattcgccg actacctgcg gccaagcctc aggctggcgg ccctgatggg cctgccggtc
      601 gtctatgtga tgacccacga ctcggtcttc gtcggcgagg acggcccgac gcaccagccg
      661 atcgagcacc tggcctcgct acgctccatc ccgaacgtgt tggtgctcag gccggccgac
      721 gcggaggaga ccgccgaggc ctgggcctgg gcgatggcca ggacgaccgg cccgaccgtc
      781 atagcgttga ccaggcagaa cctcccggtc ttcgagaagg ccgacccgat gtggcgcgac
      841 accttccggg ccggcgccta cgtcgcgcgc accacgaggg gcgagccgga cgtcgtcgtg
      901 ctcgcctccg gtagcgaggt cgagctggcg ctcaaggcgg cggagctcgc ctcgtccaag
      961 tcggtgcgcg tcgtgtcggt accgtcgctc gagaccttcc tggcccagcc cgacgccctg
     1021 cgcgacacga tcgcgccgcc cgaggcccgc atcgtcgcct gcgaggccgg acggtctatg
     1081 ccctggaacg gcgtggccga cggcttcctc ggcatcgagc gcttcggcga gtcgggcccg
     1141 ggcgccaagg tcgccgccca cctcggcctg acgctcgagg ccttggctgc gctgattaac
     1201 gcataacgaa gcggcagccc cggttacgcc cggggctacc gcatatacga ggagggccgc
     1261 cggcgattat cgccggcggc cctccgtttc tggctaaacc cgatcgttgg ccgactacca
     1321 ccagccgaag gcggtgccgc agcgggtcat gtaccagacg gtgacgatgc cgacgacgac
     1381 ggcgtaggag aagtaccaga tcgccaaggc ctcgccgtcg cgcttggaga gcaggccgtt
     1441 gaacagggag ccggccaggc agacgacgaa gccgagcagg gccagcacgc cgtagaacgt
     1501 gttaggcgcg gcgagggcgt cgagcgagaa cacgaacgag aagacgaaaa taaagatgaa
     1561 gagcgtgaaa ttgatcaagc cgttcctggt ttccatggca atcgactcct tttacgtatc
     1621 tggagatgtc ggatcgtagt atactccgac gagtagcact tgtccatctt gatcgaggcc
     1681 cggacctcgc cgaggaggag agcatgaaga aggcttgcgt gattctggct aacggctgcg
     1741 aggaagtcga ggctatcacc ccgatcgact acctacgtag ggccggcgtc gaggtcgtgg
     1801 cggccgggct ggacggccgc gaggccgtcg gggcgcacgg cctgatcatc ggggccgacg
     1861 ccgtactcga cgacatcgac gacgaggagt tcgactgcat cgtcgtgccg ggcggcggg
//
LOCUS       DAOOLN010000321         1919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_75818_length_1919_cov_3.166845, whole genome shotgun sequence.
ACCESSION   DAOOLN010000321 DAOOLN010000000
VERSION     DAOOLN010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75818_length_1919_cov_3.166845"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(294..693)
                     /gene="rnpB"
                     /locus_tag="PLW80_04900"
     ncRNA           complement(294..693)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLW80_04900"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(698..1507)
                     /locus_tag="PLW80_04905"
     CDS             complement(698..1507)
                     /locus_tag="PLW80_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HPB65876.1"
                     /translation="MERVAIEKDVRELPKSIAAQEEVLTRARKSYGDRSLRLEETQSR
                     VGQLRAELADVVAHKDKSEQQMDSITNPREFEALNKEITEAGLKEDQLRKDLKREEEN
                     LREAEVEVRASETLISSQEAEIAEKRERITNEAASMSAKVEKLKRDEAKTTAEIDPEV
                     VFKFERIIKSKQGVGIVPVKGVVCSGCSMILPAQFVNEVRQSDKIVFCPYCSRVLFYQ
                     EGDESQEDMIVDIESGSLADLDDFGDESDEEYDDESRRVRQGFDAGLGRLG"
     gene            complement(1648..>1919)
                     /locus_tag="PLW80_04910"
     CDS             complement(1648..>1919)
                     /locus_tag="PLW80_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPB65877.1"
                     /translation="DLSSENPFEVTEGHLLKERLREILETLTDRERQVVDFRFGLSDG
                     CSRTLEEVGRLFNVTRERIRQIEAKALKKLRHFKRSQKLRDYVDH"
BASE COUNT          301 a          630 c          562 g          426 t
ORIGIN      
        1 acgcctcgca ggcctgggcg accgctagat agtccttgga ggcccagagg gcgaggacgt
       61 cctgttggct aaccttaggc gacgagcgtg ccgtcgcgat ggccgatacg cacaaggata
      121 cgcctacggc ggtggccgtt acgccgagca tgactgccaa gcctatgacc aacctgcgac
      181 gcctgcgctt ccgtagatat gggtcggtag ttctggcggt gccgaaggat atatcgttca
      241 tactactcgg ttcgttgtat cacgccagcg ccgctatagt caattaaagc aaaaaaaggg
      301 acgaacctat aagccgggtt ctgtaccgct ttcgcggcgg ccgccatcca tctgcgccgg
      361 ggatcgctcc cctggcttcc gcgacctacc cacggagtgt cgcctcgacc tcgcggtcga
      421 ggcgcggcgc gagccgcgcc tccgttgttc ggccttgctc gcgacgaggc ttgccgtgcc
      481 gttcgcgtcg ccgcgaacgc ggtgggctct taccccacct tttcaccctt acccgcgcct
      541 ttcggcgccg gcggttcgct ttctgttgcg ctatctgtcg cgcggccgtc accggccgcg
      601 ctcccggtcg ttaaccggcg tcgcgcccta ttgagcccgg actttcctcc gcgccagccc
      661 gggggctaac gcggcggcgg cccggtccgt ccctatacta ccctagtcgt ccgagtccgg
      721 catcgaatcc ttgtcggact cgtcgtgact cgtcatcgta ctcctcgtcg gactcatcgc
      781 cgaaatcgtc cagatcggcc agagagccgg attctatatc gacgatcatg tcttcctgcg
      841 actcgtcgcc ttcctgatag aacagcacgc gagagcagta cgggcagaat acgatcttat
      901 cgctctgcct gacctcgttg acgaactggg ccggcagtat catcgagcat ccggagcata
      961 cgacgccctt gacgggtacg ataccgacgc cctgcttcga cttgatgatg cgctcgaatt
     1021 tgaacacgac ctccgggtct atttcagcgg tggtcttggc ctcgtcacgc ttgagcttct
     1081 cgaccttggc ggacatcgaa gcggcctcgt tcgtgatacg ctcacgcttc tcggcaatct
     1141 cggcctcctg ggatgagatc agggtctcgc tggcgcgtac ctcgacctcc gcctcgcgga
     1201 ggttctcttc ctcgcgcttc agatccttac gcagctggtc ttccttgaga ccggcctcgg
     1261 tgatctcctt gttcagggcc tcgaattcgc gcgggttcgt gatggagtcc atctgctgct
     1321 cggacttgtc cttgtgggct acaacgtccg ccagctcggc gcgaagctgg ccgaccctgg
     1381 actgcgtctc ctcgaggcgc aagctcctgt caccgtagct cttccgggct ctcgtgagca
     1441 cctcttcctg ggccgctatg gacttgggga gttcgcgtac gtccttttcg atggcgacgc
     1501 gctccatgag gagctcctga aacgttcgta gcctctcgaa aagctcttcc atcagcatat
     1561 gttttcacct cgtcctggat aatttcgcaa gtaacgcggg attatagcac gcggggcgcg
     1621 cggagatcaa cgacgcgctc gcgacgacta gtggtcgacg tagtctcgta gtttctggct
     1681 acgcttgaag tgccggagct tcttgagcgc cttggcctcg atctggcgta tgcgctcgcg
     1741 cgtcacgttg aacagccggc cgacctcctc gagggtgcgg ctgcacccgt cggaaaggcc
     1801 gaagcggaag tcgaccacct ggcgctcgcg gtcggtcagc gtctccagta tctccctgag
     1861 gcgctccttc aggagatgcc cctccgtcac ctcgaacggg ttttcgctcg agaggtcgg
//
LOCUS       DAOOLN010000322         1919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_75819_length_1919_cov_3.158262, whole genome shotgun sequence.
ACCESSION   DAOOLN010000322 DAOOLN010000000
VERSION     DAOOLN010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75819_length_1919_cov_3.158262"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PLW80_04915"
     CDS             <1..312
                     /locus_tag="PLW80_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPB65878.1"
                     /translation="HEPIDLTYPKANEGNPGYLPEERAWMTRFLSAGYADTFRRMHPG
                     ESGHYSWWSYRMKAREKDIGWRLDYHCVDETLFPAVRHAAIGKAVMGSDHCPVTLSLE
                     L"
     gene            337..894
                     /locus_tag="PLW80_04920"
     CDS             337..894
                     /locus_tag="PLW80_04920"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPB65879.1"
                     /translation="MRIRYNAPTTLTFTLICAIVLLFDSLLSGLTASWFSVPGRGGFD
                     PQSMRSYVRLFSHILGHADINHFLSNFSFILLLGPILESAYGSGGMAIMTLITAFVTG
                     ILNILLFQTGLMGASGIVFMMILLSSFTNFNKGEIPLTFILVLVLYLGKEVFNAFRDN
                     NISEFGHIIGGLCGSIFGYLRPARR"
     gene            1020..>1919
                     /locus_tag="PLW80_04925"
     CDS             1020..>1919
                     /locus_tag="PLW80_04925"
                     /inference="COORDINATES: protein motif:HMM:NF025022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPB65880.1"
                     /translation="MADSSERKVFVVDTNILIHNPESVLSFRDNEIVIPLQVLEELDH
                     LKTYSDQRGKSARDAIRFLDGVSKRGDLHDGVKLENGSILRVSLAQSDTAPKGFDTAK
                     NDNRIILCAAAIKESGRQVFFVSKDINARVKATALGVKAVDYEKEKVDIATLYQGVVE
                     VDVASDAIAALDATGNLPWKEAQRPNEFVLLRERGGAAERLARYRPDEGFVEALQGEL
                     GSVYGVSPLNARQRVALELLLDDRLSLVTIVGKAGTGKTLLAIAAGLRKVFDDKVYGK
                     MLVTRPVVPVGKDIGYLPGEKQAK"
BASE COUNT          363 a          636 c          576 g          344 t
ORIGIN      
        1 cacgagccca tcgacctgac ctatcctaag gccaacgagg gcaatcccgg ctacctaccc
       61 gaggaacggg cctggatgac ccgcttcctg tcggccggct acgccgatac cttcaggcgc
      121 atgcatccgg gcgagtccgg tcactacagc tggtggagct accggatgaa ggcccgagag
      181 aaggacatag gttggcgcct cgactaccac tgcgtcgacg agaccctatt cccggcggtc
      241 cgccatgcgg ccatcggcaa ggcggtcatg ggctcggacc actgcccggt aacgctatcg
      301 ctcgagcttt gattccgcca tacgctggag aacgatatga ggatacgcta taacgcgcca
      361 accacgctga ccttcaccct gatatgcgcc atcgtcctgc tgttcgatag cttgttgtcg
      421 gggctgacgg cctcctggtt ctcggtgccc gggcgcggcg gcttcgaccc gcagtcgatg
      481 cgttcgtacg tgcggctctt cagccacata ctcgggcacg ccgatatcaa ccatttcctg
      541 tccaatttct cgttcatcct gctgctcggg cccatactcg agtcggccta cggctcgggc
      601 ggcatggcca tcatgaccct gatcaccgcc ttcgtgaccg gcatcctgaa catcctccta
      661 ttccagaccg gcctgatggg cgcgagcggc atcgtgttca tgatgatact cctgtcgtcg
      721 ttcaccaact tcaacaaagg cgagataccg ctcaccttca tcctggtcct cgtcctctac
      781 ctcggcaagg aggtgttcaa cgccttccgg gacaacaaca tcagcgaatt cggccacatc
      841 ataggcgggc tctgcggcag catcttcggc tacctccggc cggccaggcg ctgacggcgg
      901 ccaggcgctg acggcggcca ggcgctaacg gtggccagac ggcggccagg cggccttgcg
      961 cgcctagctc ctcgcgctat acttgacgca tggcgcgaaa cgcctgacta gggggcacca
     1021 tggctgattc cagcgagcgt aaagtcttcg tcgtcgacac caacatactc atccacaacc
     1081 cggaatcggt actgtccttc agggacaacg agatcgtcat cccccttcag gtgctcgagg
     1141 aactcgacca cctgaagacc tattcggacc agcgcggcaa gagcgcgcgc gacgcgatac
     1201 gttttctcga cggcgtctcc aagcggggcg atctccacga cggcgtcaag cttgaaaatg
     1261 gctccatctt gcgcgtatcg ctcgcccagt cggatacggc cccgaagggc ttcgatacgg
     1321 ccaagaacga taaccggatc atcctgtgcg ccgcggcgat caaggaatcc gggcgccagg
     1381 tcttcttcgt gtccaaggac atcaacgcca gggtcaaggc tacggccctc ggcgtcaagg
     1441 cggtcgacta cgagaaggag aaggtcgaca tcgccacgct ctaccagggt gtcgtcgagg
     1501 tcgacgtggc gtcggacgcg atagccgcgc tcgacgccac cggcaacctg ccctggaagg
     1561 aggcgcagcg tcccaacgaa ttcgtgctac tccgcgagcg cggcggcgcg gcagagcgcc
     1621 tagcccgcta taggccggac gagggtttcg tcgaggcgct gcagggggag ctcggctcgg
     1681 tctacggcgt ctcgcccctc aacgccaggc aacgggtggc cctcgagctg ctgctcgatg
     1741 ataggctcag cctcgttacc atcgtcggca aggccggcac cggcaagacg ctgctcgcga
     1801 tcgcggccgg cctccgtaag gtgttcgacg acaaggtata cggcaagatg ctcgtaacga
     1861 ggccggtcgt gccggtcggc aaggatatcg gctacctgcc tggcgagaag caggccaag
//
LOCUS       DAOOLN010000323         1918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_75882_length_1918_cov_3.244230, whole genome shotgun sequence.
ACCESSION   DAOOLN010000323 DAOOLN010000000
VERSION     DAOOLN010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75882_length_1918_cov_3.244230"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PLW80_04930"
     CDS             <1..723
                     /locus_tag="PLW80_04930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPB65881.1"
                     /translation="DSTVKVYPYVAGKLVSSKASLGSRVTKGQTIAEIDPSTPGSAYT
                     LNAVYAPISGTVTSLPVSVGSTVGTATAIAEIGSIDELEVEARIPERYVGVLKVGLRA
                     TVSLEAYPGVSFAATVSSVSPVVDSTSRTKTIRLAFDRYDERVNAGMFAKLRLDTVAY
                     PDCLAVASGGVLQDSTGHYLFVVKDGSTVEKRRVEVGVDIDGLAAITDGLSEGELVVV
                     EGASVLADGARIKDISSPGSGT"
     gene            720..>1918
                     /locus_tag="PLW80_04935"
     CDS             720..>1918
                     /locus_tag="PLW80_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPB65882.1"
                     /translation="MSVAKKVVERPVLVAIAFALITVVSLYTLSDLALDLMPDTERPV
                     LMVSASYPGASPESVEKSVTAALESALINVSGLQSLSSTSSEGSSRLELEFEYGTDLD
                     TATNDVRDKLDRIRRSLPDDASDPQIFRFSSSDMPIMRIAVSGERSSEELKVIAEDII
                     QPRLEQIDGIAQADVNGGRERIVRVELSQNRLDAYGLTITGIASKLASANVELGGGSI
                     GEGTKNYLIRTTGEYSSVDEIASTVVGTAGGYAVRLSDLATVAMGYEDESRLVYVNGE
                     PGVYVSLTKRSGSNSVKAADAVYEKLEEIRPLLPSDVSMKIVSDDTLSIRSTLSNLLS
                     SALQGALLAMAVPFLFLRSLKSTVIIGVSIPFSILVTLLCMYFAGITLNMMTMTGLIL
                     GVGMIVDA"
BASE COUNT          338 a          665 c          600 g          315 t
ORIGIN      
        1 gactccaccg tcaaggtcta cccctacgtc gccggcaagc tggtatcgtc gaaggcgtcc
       61 ctcggatccc gcgtgacgaa gggccagacg atagccgaga tcgacccctc gacgcccggc
      121 tccgcctata cgctcaacgc ggtctacgcg ccgataagcg gcacggtgac ctcgctgccg
      181 gtatcggtcg gctcgaccgt cggtaccgcc acggccatag ccgagatagg ctcgatcgac
      241 gagctcgagg tcgaggccag aataccggag cgctacgtcg gcgtgctcaa ggtggggctc
      301 agggcgacgg tatcgctcga ggcctacccc ggcgtcagct tcgccgcgac cgtgtcgagc
      361 gtgtcgccgg tcgtggactc gacctcgagg acgaagacca tccgcctggc cttcgaccgc
      421 tacgacgagc gcgtcaacgc cggcatgttc gccaagctca ggctcgacac ggtggcctac
      481 cccgactgcc tggccgtcgc gtctggcggc gtgctccagg attcgaccgg ccattacctc
      541 ttcgtcgtca aggacggctc gaccgtcgag aagcgtcgcg tcgaggtcgg cgtcgacatc
      601 gacggcctcg cggcgatcac cgacggactg tccgagggcg agctcgtcgt cgtcgagggc
      661 gcgtcggtgc tagccgacgg cgccaggatc aaggatattt cgtctccggg gtccggcaca
      721 tgagcgttgc caaaaaagtc gtcgaacggc cggtcctggt cgcgatcgcc ttcgcgctaa
      781 ttacggtcgt cagcctctac accctatccg acctggctct cgacctgatg ccggacacgg
      841 agcggccagt cctgatggtc agcgccagct accccggggc gagtccagag tcggtcgaga
      901 agtcggtaac ggccgcgctc gagagcgcgc tgatcaatgt ctccggcctc cagagcctct
      961 cgtcgacctc cagcgagggt tcctcgaggc tggagctcga attcgagtac ggcaccgacc
     1021 tcgacaccgc gacgaacgac gtgcgcgaca agctggaccg gatacgccgt agcctgcccg
     1081 acgacgcgtc cgacccgcag atcttccggt tctcgtcgag cgacatgccc atcatgcgca
     1141 tagccgtctc gggcgagcgt agctcggagg agctcaaggt catagccgag gacatcatcc
     1201 aacccaggct cgagcagatc gacggcatcg cgcaggcgga cgtcaacggc ggccgcgagc
     1261 gcatcgtacg cgtcgagctg tcccagaacc gcctggacgc ctacgggctg accatcaccg
     1321 gcatcgcctc gaagctggct agcgccaacg tcgagctggg cggcggtagc atcggcgagg
     1381 ggaccaagaa ctacctgatc cgcacgaccg gcgaatactc cagcgtcgac gagatagcct
     1441 cgaccgtcgt cggcacggcg ggcggctatg ccgtccgcct gtccgacctc gccacggtgg
     1501 cgatgggcta cgaggacgag agccgcctcg tctatgtcaa cggcgagccg ggcgtctacg
     1561 tgtcgctgac caagcgctcg ggctcgaact ccgtcaaggc cgccgacgcc gtctacgaga
     1621 agctcgagga gatcaggccc ctcctgccct ccgacgtgag catgaagatc gtctcggacg
     1681 acacgctatc catcaggagc acgctgtcga acctcctgtc gtccgccctg cagggcgccc
     1741 tgctcgcgat ggccgtcccg ttcctgttcc tcaggagcct caagagcacc gtcatcatcg
     1801 gcgtctcgat acccttctcc atcctggtca cgctgctgtg catgtacttc gcgggcatca
     1861 ccctcaacat gatgacgatg accggcctga tcctcggcgt cggcatgata gtcgacgc
//
LOCUS       DAOOLN010000324         1909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_76390_length_1909_cov_3.712513, whole genome shotgun sequence.
ACCESSION   DAOOLN010000324 DAOOLN010000000
VERSION     DAOOLN010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76390_length_1909_cov_3.712513"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..1592)
                     /locus_tag="PLW80_04940"
     CDS             complement(249..1592)
                     /locus_tag="PLW80_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPB65883.1"
                     /translation="MVFFNYPSAWSSLSTFPAAETIPATGAFFVAACVSMFACSFIGD
                     GLLGVAAVLAGLMASGSAIAMAAWPGTIPLGAIAVAMGAAAGLTYAIGLLFLVHRAPA
                     PFDPRIVGVCIASFSAVSGVLLALLRIRGAAVALAVALPLCSAAAGLRVRRSVEREPI
                     EERGLPVRPALGLVYLIALNYIAYNAATVTSIYGSAGFIAGKPALDWAFRAFVAIAAV
                     ATIRRFNVVKMLYGSYIMIMAALAFSVMGPRGRLPYGLLINTAFVLTEFGYASLLVRL
                     GRGSKARPALFALGTLVVALPSLVGNHLGAWLSPRLGEASLLTIGAGLALYCVALPFV
                     PGLLRSIEGAFAATPAGGDDGQESFIRETFFSRLPAERSLDAIESSYDDDYRLSPRER
                     EVAALLLDRYDYDAIAARLGISLNTVKSHARSIYRKYDIRGRKDLIALSANATNG"
BASE COUNT          348 a          628 c          635 g          298 t
ORIGIN      
        1 cggccgcgtc ggccggcgtg gcgctcaggt tggcggggcg gtccggtttc tcgctcatct
       61 cgaggttgac gttctggcac gctatgagcg taaggacgat cgcgatcgtc tttacacagg
      121 ctcgagcgtg gcgcggcaat aagccgtgat ccatgggaac ctcctgacgg ccggcgctcg
      181 gcgccgtcca tcgtccatag gtatcccggt acccggccgc tttcaatcac acgaaaagtg
      241 tgagcccatc acccgttcgt ggcgttggcc gagagggcga tgaggtcctt ccttcctcgg
      301 atgtcgtatt tccggtagat ggacctggcg tggctcttga cggtattcag ggatatcccg
      361 agcctcgccg cgatggcgtc gtaatcgtaa cggtcgagca gcagtgcggc aacctcccgc
      421 tccctcgggc tcagccggta atcgtcatca tagctcgact cgatggcgtc gagcgaccgt
      481 tccgcgggga gcctggagaa gaacgtctcg cggatgaacg attcctggcc gtcgtcgccc
      541 ccggcgggcg tcgccgcgaa ggccccctct atggagcgca ggagcccggg cacgaagggc
      601 agcgcgacgc agtacagcgc cagtccggcg ccgatggtca gcaaggacgc ctcgccgagg
      661 cgtggcgata gccaggcgcc caggtggttg ccgacgaggc tcggcagcgc gacgacgagc
      721 gtgccgaggg cgaacagcgc tggccgagcc tttgatccgc gcccaagccg gacgagtagc
      781 gacgcatagc cgaattccgt aagcacgaag gccgtattga tcaggagccc gtacgggagg
      841 cggcctctcg gacccattac ggaaaaggcc agggccgcca tgatcatgat atagctgccg
      901 tacagcatct tgacgacgtt gaagcgacgt atcgtagcta ccgccgcgat ggcgacgaag
      961 gccctgaacg cccagtctaa ggcgggcttg ccggctatga accccgcgct cccgtagatc
     1021 gaggttaccg tcgcggcgtt ataggcgata tagttcagcg cgatgaggta gaccaagccc
     1081 agggccggcc gtacaggcag gccgcgttcc tcgatcggct cgcgctcgac ggagcgccgg
     1141 acgcgcagcc cggccgccgc gctacagagc ggtagcgcga cggcgagcgc gacggccgcg
     1201 ccacgtatcc gtagtagcgc cagtaggacg ccggataccg cgctgaagga ggcgatgcac
     1261 actccgacga tcctcgggtc gaacggcgcc ggcgcccggt gcacgagaaa caataagcca
     1321 atggcgtagg taagcccggc ggcggcgccc atggcgacgg ctatggcgcc gagcgggatc
     1381 gtcccgggcc atgcggccat ggcgatagcg gagccggacg ccatgagtcc cgctagaacc
     1441 gcggccaccc cgagcagtcc gtcgccgatg aacgaacagg cgaacatcga tacgcaggcg
     1501 gccacgaaga acgcgcccgt cgccgggatc gtctccgcgg ccgggaacgt cgagaggctg
     1561 gaccaggcgc tcggataatt gaagaacacc atggcccacc cgtatccgaa agccgcgtac
     1621 gctaccgccg gccccgcgcc ggctccagcg cccggcgaac gcatcgtctg ggtcatacct
     1681 acgtcgctcc gtcgcgacgt tcgccccggg gctggctcga cgaaggcccg ggggcgaccg
     1741 atcgcggatc aggatctgtt agctcgtatg gtataccatg gctttcggtg gctggtaacg
     1801 aaaaaaaaca ccaccgttcg ccgctactct ggccctcccg ctatcgcccg gccatcgcct
     1861 cgtatatgct ccgttcaagc gcccagacgt cgtcgcactg gttgctctc
//
LOCUS       DAOOLN010000325         1909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_76398_length_1909_cov_3.278317, whole genome shotgun sequence.
ACCESSION   DAOOLN010000325 DAOOLN010000000
VERSION     DAOOLN010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76398_length_1909_cov_3.278317"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="PLW80_04945"
     CDS             <1..973
                     /locus_tag="PLW80_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65884.1"
                     /translation="EDISPQEERAFLIEIARLSASLLDDVGSQVRASLERFHAGIAGV
                     NEVVSSVESSRSAIIAGRRAIDHDDLRDRTRAYMAAKERAVLEASEISESVRKLDERF
                     KEMDAILARFKSIVTASRIETARNKALAIVANTVGGMMDLTERLAGDVSSAGAVTRSF
                     GKTFGAGMSDYLFNASDGLETLKEEIARLEDEGKRIVESRRRLWETESEFKPFSDEFM
                     QAIDEASASVGRIDSLAGELEGMRDAIVAQVSASVAEAGASSAVQPSSAVQPSSARTS
                     IHSARLKSIVDRFTIFAHKQTAARVTRLEAGSGADESSAESGEVTLF"
     gene            1001..1579
                     /locus_tag="PLW80_04950"
     CDS             1001..1579
                     /locus_tag="PLW80_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HPB65885.1"
                     /translation="MYEHKLSRFDQSVLTIHPGEYHVSRDDIIISTVLGSCVSVAFWD
                     RRLQQGGMNHFMLPGDVSDADLVRNPNAKYGMFAIELLYNELMKLGSMKRDLVAKVFG
                     GASVLKLETGPTKVPKGNVDFAQAYLHAEGIPIIANDTGGILPRKIFFFAQTGKVLLK
                     RIAGAMASEVEREEALYLDKIKRAESGKVTLF"
     gene            complement(1620..>1909)
                     /locus_tag="PLW80_04955"
     CDS             complement(1620..>1909)
                     /locus_tag="PLW80_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HPB65886.1"
                     /translation="GVVIGETTVIGRDVKLYQGVTLGALSVRKGASGQKRHPTIEDGV
                     TIYSGATILGGDTTIGAGSVIGGNVWLTSSLPPGSRVYMQQGDSVVEGRRQ"
BASE COUNT          350 a          634 c          605 g          320 t
ORIGIN      
        1 cgaggatatc agcccccagg aggagcgcgc cttcctgatc gagatagcga ggctatcggc
       61 ctccctgctc gatgatgtcg gctcccaggt ccgggccagc ctcgagcgct tccacgccgg
      121 aatcgctggc gtcaacgagg tcgtctcctc ggtcgagtcg agccgctcgg ctatcatcgc
      181 cggcaggcgt gcgatagatc acgacgactt gcgtgatagg acgcgcgcct acatggccgc
      241 caaggagcgg gccgtactcg aggcctcgga gatcagcgaa tcggtacgca agctcgacga
      301 gcgcttcaag gagatggacg cgatcctcgc caggttcaag agcattgtca ccgcctcgag
      361 gatagagacc gcgcgcaaca aggccctggc catcgtcgcc aacaccgtcg gcggcatgat
      421 ggacctgacc gaacgcctgg ccggagacgt ctcgtccgcc ggggcggtca cgaggtcctt
      481 cggcaagacc ttcggcgccg gcatgtccga ttacctgttc aacgcgtcag atggcctcga
      541 gaccctcaag gaagagatag ccaggctcga ggacgaggga aagcgcatcg tcgagtcgcg
      601 ccggcgcctc tgggaaaccg agtctgaatt caagccgttc tcggacgaat tcatgcaggc
      661 catcgacgag gcctccgcgt cggtagggcg catcgactcg ctcgccggag agctcgaggg
      721 catgcgggac gccatcgtcg cccaagtctc cgcgtcggtc gccgaggcgg gagcgtcgtc
      781 tgccgtgcag ccgtcgtctg ccgtgcagcc gtcgtccgcg aggacgagca tacacagcgc
      841 gcggctcaag tccatcgtcg accgcttcac gatcttcgcc cataagcaga ccgcggcccg
      901 cgttaccagg ctggaggcgg gatcgggagc cgatgaatcg agcgccgaat ccggcgaagt
      961 cacgctgttc tgatgcgtgc cggcaacgcc tggaggcctc gtgtacgagc ataagctgag
     1021 ccgcttcgac cagagcgtcc taaccatcca cccgggggag taccacgtct cacgcgacga
     1081 tatcatcata tcgaccgtgc tcggctcctg cgtctctgtg gcgttctggg atcggcgact
     1141 ccagcagggc ggcatgaatc acttcatgct gcccggcgac gtctccgacg ccgacctggt
     1201 gcgcaacccg aacgccaagt acggcatgtt cgccatagag ctcctgtaca acgagctgat
     1261 gaagctaggc tcgatgaagc gcgacctcgt ggccaaggtg ttcgggggcg cttcagtcct
     1321 caagctcgaa accggcccga ccaaggtgcc caagggcaac gtggacttcg cgcaggccta
     1381 tctgcacgcc gagggcatac ccatcatagc caacgacacg ggcggcatcc tgccgcgcaa
     1441 gatcttcttc ttcgcccaga ccggcaaggt gctgctcaag cgcatcgccg gggccatggc
     1501 ctccgaggtg gagcgcgagg aggcgctcta cctggataag atcaagcgcg cggagagcgg
     1561 taaggtcacc ctgttctgag ccactattgg cgacgacggc gctattggcg acgacggcgc
     1621 tattggcgac gaccttcgac cacggagtct ccctgctgca tgtataccct cgagccgggc
     1681 ggcaaggacg aggtcagcca cacgttgccg cctatcacgc tacccgcgcc tatcgtggtg
     1741 tccccgccga gtatcgtggc gcccgagtag atggtcacgc cgtcctcgat ggtcgggtgc
     1801 cgcttctggc cgctcgcccc cttgcgcacc gacagcgcgc cgagcgtcac gccttggtat
     1861 agcttgacgt cccggcctat cacggtggtc tcgcctatga ccacgccgg
//
LOCUS       DAOOLN010000326         1908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_76488_length_1908_cov_2.463573, whole genome shotgun sequence.
ACCESSION   DAOOLN010000326 DAOOLN010000000
VERSION     DAOOLN010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76488_length_1908_cov_2.463573"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PLW80_04960"
     CDS             <1..480
                     /locus_tag="PLW80_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPB65887.1"
                     /translation="PRSVRQKTREIEEAIVQTEAKLGRPATDQEIAESLGLSDDEFSK
                     TMLKISSTSILSLNDVWYTADESEHMTIGDSVESPGSMSPDSITEREEIRRVIVEAIQ
                     ELPDKEKKVLVLYYYEELTLKEIGKVLEVTESRISQLHTKAILRLRARLTHYRKGII"
     gene            482..>1908
                     /locus_tag="PLW80_04965"
     CDS             482..>1908
                     /locus_tag="PLW80_04965"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HPB65888.1"
                     /translation="MEARIGFDAVRELMRRYLDEDGDKRAIVAEGATLEEALRSASVQ
                     LDCPVARLEYDILERGAKGFVGLGRKKWKLSVYEAAVKQKVEESSIESFDGGLDLPIE
                     LKPIQQDVDGQVYVRLATEGALLKVAEPQGRGRRATDKQAVEKLHARAIHDFDEALVR
                     ETVKRAAGEWVVVGDFIANPAADALLTVDIGSQEMEARVIITPPQPGGCDLSKDVILA
                     YLRNNKVVFGVIEEALQGLEDAPRYKEPVLVAQGQRAVNGDDARIQFLFETDRTKLNL
                     EEKNGKVDYKELHLVQNVVEGQALAKKVQAKQGVPGRTVTGKLLPAKNGKDLPLPLGK
                     NVHESDDGLTVIADVNGEATYINSKINVETVYTINGDVDLKTGNQFFLGTIVVVGNVE
                     DGFSVKATGNIEVRGNVGKAEISAEGDVIVHQGITGKGAGVITAGKNVWAKFIENAQL
                     YAGENVIVQTSIINSEITAEKRILCS"
BASE COUNT          424 a          609 c          604 g          271 t
ORIGIN      
        1 ccgcgctcgg tgcgtcagaa gaccagggag atcgaggaag ccatcgtaca gaccgaggcc
       61 aagctcggcc gccccgctac ggatcaggag atcgccgagt cgctcggcct gtccgacgat
      121 gaattctcca agacgatgct caagatatcg agcacctcga tcctgtcgct caatgacgtc
      181 tggtataccg cggacgagtc cgagcacatg acgataggcg acagcgtcga gagccccggc
      241 agcatgagcc ccgactcgat caccgagcgc gaggagatac gacgcgtgat cgtcgaggcc
      301 atccaggagc taccggataa ggagaagaag gtcctcgttc tctactatta cgaggagttg
      361 acgctcaagg agatcggtaa ggtgctagag gtaaccgaat cgaggatcag ccaactccat
      421 acgaaggcca tcctgcgcct acgcgcgcga ctgacgcact accggaaggg catcatttaa
      481 tatggaggcg cgcataggct tcgacgcggt ccgcgagctg atgcgccgtt acctggacga
      541 ggacggcgac aagcgcgcga tagtagccga gggcgccacg ctcgaggaag cgctcaggag
      601 cgcttccgtc cagctcgact gcccggtggc caggctcgag tacgacatac tcgagcgtgg
      661 ggcgaagggc ttcgtcggcc tgggccgaaa gaaatggaag ctgagcgtct acgaggccgc
      721 ggtcaagcag aaggtcgagg agtcgtcgat cgagtccttc gacggcggcc tcgacctgcc
      781 gatcgagctc aagcccatcc agcaggacgt cgacggccag gtctacgtgc ggctcgccac
      841 cgaaggcgcc ctgctcaagg tagccgagcc ccagggcagg ggccgccgcg ccaccgacaa
      901 gcaagccgtc gagaagctcc acgcgcgcgc gatccacgac ttcgacgagg cgctcgtgcg
      961 cgagacggtc aagcgggcgg ccggggaatg ggtggtcgtc ggcgacttca tcgccaatcc
     1021 cgccgccgac gcgctcctga ccgtcgacat cggctcccag gagatggagg cccgcgtcat
     1081 aatcacgccg ccgcagcccg gcggatgcga cctgtccaag gacgttatac tcgcctacct
     1141 gcgcaacaac aaggtcgtct tcggcgtgat cgaggaggcg ctccagggcc tcgaggatgc
     1201 tcctcgctac aaggagcctg tcctcgttgc ccagggacag cgcgccgtca acggcgacga
     1261 tgcccgcatc caattcctgt tcgagaccga ccgcaccaag ctgaacctcg aggagaagaa
     1321 cggcaaggtg gactacaagg agttgcacct cgtccagaac gtggtcgagg ggcaggccct
     1381 ggccaagaag gtccaggcca agcagggcgt gccgggccgt accgtgaccg gcaagctctt
     1441 accggccaaa aacggcaagg acctgccgct tccgctcggc aagaacgtcc acgagagcga
     1501 cgacggcctg accgtcatag cggatgtcaa cggcgaggcc acctacatca acagcaagat
     1561 caacgtcgag accgtctata ccatcaacgg ggacgtcgac ctcaagaccg gaaaccagtt
     1621 cttcctcggc accatagtcg tcgtcggcaa cgtcgaggac ggcttctccg tcaaggccac
     1681 cggcaatatc gaagtgcgcg gcaacgtcgg caaggccgag atcagcgccg aaggcgacgt
     1741 catcgtccac cagggcatca ccgggaaggg cgcgggcgtg atcacggccg gcaagaacgt
     1801 ctgggccaag ttcatcgaga acgcgcagct ctacgccggc gagaacgtca tcgtccagac
     1861 cagcatcatc aactcggaga tcaccgccga gaagcgcatc ctgtgctc
//
LOCUS       DAOOLN010000327         1906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_76575_length_1906_cov_3.104808, whole genome shotgun sequence.
ACCESSION   DAOOLN010000327 DAOOLN010000000
VERSION     DAOOLN010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76575_length_1906_cov_3.104808"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1848)
                     /locus_tag="PLW80_04970"
     CDS             complement(<1..1848)
                     /locus_tag="PLW80_04970"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPB65889.1"
                     /translation="MALFSGSDANGALSFGSPMVREPAEAAFPDYAKAPIRASGAASL
                     SIAFSGVSPDPKKPLAFWGYGPGELTQPLALKKGAAPDLWTAAVKLAPRPDGRSDVWV
                     AVTEASGELAYARAALEYDSTPPAIEILAPAAVDGKAATPGAFTLVVRADDVAGIASL
                     SYASGSESGEFERTPGSGDAAKRFTFPPKAGGATIHVRAVDGSGNRSQTTLTVAYDAV
                     ADAPKVRFLSPAAGAELDAGPAEAFIYATDDDLLASATLVVDGATVEASGPGPLYSLA
                     LGEAKRGQRSAQAWATDSDGVQSAKATIAFVQRAAAPILSFTSAIAPVAGAAPEPSAF
                     QAGSALVADGKTILVASAMAANGLAKAEWALNDGAWSSLPISKPAADGSYELRLALPA
                     GLAYERNLVRLRVSDAFGQAAEAETSFYRVAPERDLERTAGEGVYLYDERVADGRVTL
                     APGLAIGALWYGRPVASLAVEPPVAYAEASFDGRALRLTGLADGGVGAEPVVLVVETV
                     DGERFRSSPLGLVVDSGPPALTITEPASGSPTTGSWRLAGEASDPNGLAELAWSADGG
                     LSWNRLEPGKSASYSAELSPEAIDGPVGLIVRAVDRAGAASYALTAVARD"
BASE COUNT          257 a          660 c          725 g          264 t
ORIGIN      
        1 atcgcgggcc acggccgtca gcgcgtagct cgcggcgccc gcccggtcga cggcccgcac
       61 gatgaggccg accggcccgt cgattgcctc cggcgatagt tcggcggagt acgacgcgct
      121 tttgcccggt tctagccggt tccagctcag gccgccatcg gcggaccagg ccagctcggc
      181 caggccgttc gggtcggagg cctcgccggc caggcgccac gagccggtcg tcgggcttcc
      241 cgaggccggc tcggttatgg tcaacgccgg cgggccagaa tcgactacca gcccgagcgg
      301 cgaggagcgg aagcgctcgc cgtcgaccgt ttcgacgacc aggaccaccg gttcggcgcc
      361 gacgccgccg tcggccaggc cggtcaggcg cagggcccgg ccgtcgaagg aagcctccgc
      421 gtaggcgacc ggcggctcga cggccaggct cgcgaccggc ctgccgtacc agagcgcgcc
      481 tatcgccagg ccgggcgcta gggtcacgcg accgtccgcg acgcgctcgt cgtagaggta
      541 gacgccctcg ccggccgtcc gctcgaggtc acgctccggc gcgacgcgat agaacgaggt
      601 ctcggcctcg gcggcttggc cgaaggcgtc gctaaccctg aggcgcacca ggttccgctc
      661 gtacgcgagg ccggcgggca aggccagccg tagctcgtac gagccgtcgg ccgccggttt
      721 cgagatgggt agcgacgacc aggcgccgtc gttcagggcc cactcggcct tggccagtcc
      781 gttcgcggcc atggccgacg cgacgaggat ggtcttgccg tcggctacca gggccgagcc
      841 ggcctggaac gccgaaggct cgggcgccgc gccggcgacc ggcgctatgg ccgacgtaaa
      901 ggaaagtatc ggcgcggctg cgcgctggac gaaggctatg gtcgccttgg ccgactggac
      961 gccgtccgag tcggtcgccc aggcctgggc cgagcgctgg ccgcgcttgg cctcgccgag
     1021 agcgagcgag tagagcgggc ctgggccgct cgcctcgacc gtggcgccgt cgacgacgag
     1081 cgtcgcgctc gcaaggaggt cgtcgtcggt cgcatagatg aaggcctcgg ccggcccggc
     1141 gtcgagttcg gcgccggcgg cggggctcag gaaccggacc ttgggcgcgt ccgcgacggc
     1201 gtcgtaggcc acggtgagcg tcgtctggga cctattgccg gagccatcga cggctcgtac
     1261 gtgtattgtc gccccgccgg ccttgggcgg gaaggtgaag cgcttggcgg cgtcgcccga
     1321 gccgggcgtg cgctcgaact cgcccgactc cgagcctgag gcgtacgaca gcgaggcgat
     1381 gcccgcgacg tcgtcggccc ggacgaccag cgtgaaggcg ccgggcgtgg cggccttgcc
     1441 gtctacggcg gccggagcca gtatctcgat ggccggcggt gtcgagtcgt actcgagggc
     1501 cgccctggcg taggccagct cgccggaagc ctcggtgacg gcgacccaaa cgtcgcttcg
     1561 gccgtcgggc ctgggcgcca gtttgacggc ggccgtccac aggtccggcg cagcgccttt
     1621 cttgagggcg agcggctggg tcagctctcc ggggccgtag ccccagaagg ccaacggctt
     1681 cttgggatca ggcgagacgc ctgagaaggc tatcgacagc gacgccgcgc ccgaggctcg
     1741 gatcggggcc ttggcgtagt cggggaaggc cgcctcggcg ggttcccgca ccatgggtga
     1801 gccgaacgat agcgcgccgt tcgcgtcgga gccgctgaat agcgccatcg ggccttccgg
     1861 ggttcccggc aggggccacg ttcgatttga tggactcgag cggctg
//
LOCUS       DAOOLN010000328         1905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_76637_length_1905_cov_2.900000, whole genome shotgun sequence.
ACCESSION   DAOOLN010000328 DAOOLN010000000
VERSION     DAOOLN010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76637_length_1905_cov_2.900000"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1323)
                     /locus_tag="PLW80_04975"
     CDS             complement(169..1323)
                     /locus_tag="PLW80_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB65890.1"
                     /translation="MRKRNIVAAAVVALVLASCGGEKQADVIKVGWFGALTGDQAVWG
                     NNEFNTVKMLFEEINAAGGVTVGGKAYTLEAVGYDNKGDAQEAVNVVRRLTSQDKVVA
                     ILGPNASGNAIPIAPILESAKVPDIATVATNPKVAVLDGKVKPYNFRVCFIDPYQGAV
                     AAGYAFDVLGARKAAVLYDVSDDYSQGLREFFIQNFEKKGGTIVADESFKSGDVDFRP
                     QLSKIKAQNPDIIFMPYFFKEVALSANQARELGITQVLMGGDGWPSDQLISMGGAAVE
                     GCYFVNHLDYEDPDVAHYKARYFKKYGVETELNGYLVHDAVVLLVDALKRAGEPTGEA
                     IAKALESSDVKGITGRIKISPQTHNPEGKDAAIIKIVGGKYIFQQKYAAE"
     gene            1501..>1905
                     /locus_tag="PLW80_04980"
     CDS             1501..>1905
                     /locus_tag="PLW80_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65891.1"
                     /translation="MNETMIFGNELPKRALRKADRSLKSMARRFSFDPAVYPRLSAEP
                     MPGAYEAFGVMKLEEAKPGATVVPPDPGKGIALGTIRMGFGHYRIGLAIASAARSMGL
                     TPYWLDLMSFPDSAASKTIKYLEDLYNLGSRLS"
BASE COUNT          320 a          606 c          603 g          376 t
ORIGIN      
        1 atctgttgca ggaactgagc cataagcgct ccggatttca ggcgacgagg gccgccgtcg
       61 gggatagggc ggcctgggcc ccccgtcgcg tcaaggaggg agcccccgcc tgggcgggcg
      121 ctccctccgg tcggcattca atgattgttc agaaatgctc gccccggatc actccgcggc
      181 gtatttctgc tggaagatgt acttgccgcc gacgatcttg atgatggcgg cgtccttgcc
      241 ctcggggtta tgggtctgcg ggcttatctt gatgcgcccg gtgatgccct tgacgtcgga
      301 gctctccagg gccttcgcta tcgcctcgcc ggtcggctct cccgcgcgct tgagcgcgtc
      361 gacgaggagc accaccgcgt cgtggaccag gtagccgttc agctcggtct cgacgccgta
      421 cttcttgaag taacgggcct tgtagtgggc cacgtccggg tcctcgtagt cgaggtgatt
      481 gacgaagtag caaccttcga cggctgcgcc gcccatgctg atcagctggt cgctcggcca
      541 gccgtcgccg cccatcagca cctgcgtgat gccgagctcg cgcgcctggt tggcggacag
      601 cgcgacctcc ttgaagaagt agggcatgaa gatgatgtca gggttctggg ccttgatctt
      661 ggacagctgg ggcctgaagt cgacgtcgcc ggacttgaac gactcgtcgg ctacgatcgt
      721 gccgcccttc ttctcgaaat tctggatgaa gaactcgcgg aggccctgcg agtagtcgtc
      781 ggacacatcg tagagcaccg cggccttgcg ggcgccgagc acgtcgaacg cgtagccggc
      841 cgcgacagcg ccctggtagg ggtcgatgaa gcagacgcgg aagttatacg gcttgacctt
      901 gccgtcgagc accgcgacct tcgggttggt cgcgacggtg gcgatgtccg gcaccttggc
      961 gctctcgagg attggcgcta tcgggatggc gttgccggag gcgttcgggc cgaggatggc
     1021 cacgacctta tcctggctgg tcaggcgcct aaccacgttc accgcttcct gagcgtcgcc
     1081 cttgttgtcg tagcccacgg cctcgagcgt gtaggcctta ccgcccaccg tcacgccgcc
     1141 ggcggcgttg atctcctcga agagcatctt gaccgtgttg aattcgttgt tgccccagac
     1201 cgcctggtcg ccggtgaggg ctccgaacca gccgaccttg atgacgtcgg cctgcttctc
     1261 gccgccgcac gacgcgagga cgagggcgac tacggccgcc gccacgatgt tccgtttgcg
     1321 catggtgcct ccaaaaatag atgtttctac ctatcgctat aatcgctcat tcgtcaaggt
     1381 cgcattttta tacaactctt ctgtatattt atgcaagccc tccgggtaga caacaggcag
     1441 gcttgccaaa cggctcggcg cgtatatagt cgaggtagtg gatgatgcac ggaggttcgc
     1501 atgaacgaga cgatgatctt cggaaacgag ctacccaagc gcgcgctcag gaaggccgac
     1561 cgatcgctca agtcgatggc caggcgcttc tcgttcgacc cggccgtcta tccccgactg
     1621 agcgccgagc cgatgcctgg cgcctacgag gcgttcggcg tgatgaagct cgaggaagcg
     1681 aagcccggcg cgaccgtcgt cccgcccgac cccggaaagg gcatagcgct cggcaccata
     1741 cgcatgggct tcggccacta ccgcatcggc ctggccatag cctcggccgc gcgatcgatg
     1801 ggcctgacgc catactggct cgacctgatg tccttcccgg acagcgccgc gagcaagacc
     1861 atcaagtacc tcgaggacct gtacaacctc ggctccaggc tgtcg
//
LOCUS       DAOOLN010000329         1905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_76646_length_1905_cov_2.422162, whole genome shotgun sequence.
ACCESSION   DAOOLN010000329 DAOOLN010000000
VERSION     DAOOLN010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76646_length_1905_cov_2.422162"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="PLW80_04985"
     CDS             complement(<1..493)
                     /locus_tag="PLW80_04985"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65892.1"
                     /translation="MSIDKRFIRHYVIIAALLAVAGSTLVRYARLAGGAGADRPSASA
                     SRVVSIRGPIYDREGRLLAVDTDLYDVSIWKPSFQADKVGSFARSASAALGVDPEVLA
                     GKLAEDGADFAYLARRVSGDAAKELERVIRDEGVNGLRIDRVSGRVYPERALAAHLAG
                     FVGT"
     gene            complement(480..1085)
                     /gene="lepB"
                     /locus_tag="PLW80_04990"
     CDS             complement(480..1085)
                     /gene="lepB"
                     /locus_tag="PLW80_04990"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPB65893.1"
                     /translation="MTEVEPDRWRGSPRGRLSAARIIVAAVALAVALKAFALDLVIVS
                     GESMSPTIGSGAVAVVAPCAYGLRLPLSGSYLVRWGEPAPGDIVLVSPFPPERRKAIK
                     RVFDVGPAFIVAEAGSLRGRGGVVSLGPSPRSSLAGTAYLPAGKAFVVGDNAERSIDS
                     RRYGAVPIEKLAGKVLLWFGGAAEPTLRTISSKDAAEDVDR"
     gene            1137..>1905
                     /locus_tag="PLW80_04995"
     CDS             1137..>1905
                     /locus_tag="PLW80_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPB65894.1"
                     /translation="MEMQTVIADQYIARRRKPIRISVDRGFSHSLADGRIAMKPARRE
                     SRPVTLEPSEPSWRQSIGRRAKARPNQPGGRVAKRVAVIDSISGLVEALASPRVAAPI
                     AGIAGAIALMVAIAAVGAFGRPADLTSFVLPDDGSSAAILLDALSPAADYGADSIPTP
                     ELPMTLRIATYTVAKNDTLDAISRRYGLRMDTLISVNGLGDARRIISGSTLKVPNLDG
                     VVHTVTKGESLAGIAAAKGVSVLDIVDANDLASQTILP"
BASE COUNT          350 a          655 c          623 g          277 t
ORIGIN      
        1 cggtgccgac gaagccggca aggtgggccg caagcgcgcg ttccggatac acccggccgg
       61 atacacggtc gatccggagg ccgttcacgc cctcgtcgcg tatgacgcgc tcgagttcct
      121 tggccgcgtc gcccgagacg cgccgggcca ggtacgcgaa gtccgccccg tcttcggcca
      181 gcttgcccgc aagcacttcc gggtcgacgc cgagcgctgc cgaggccgaa cgcgcgaacg
      241 aaccgacctt atccgcctgg aaggacggct tccagatcga gacgtcatag aggtcggtat
      301 cgaccgcgag aaggcggccc tcccggtcgt agatcgggcc acgtatcgag acgactctgg
      361 aggccgaggc cgatggccga tccgccccgg cgccgccggc caggcgggcg tagcgcacca
      421 gggtcgagcc ggcaacggcc agcaacgcgg cgatgatgac gtaatgacgg ataaagcgct
      481 tatcgatcga catcctcggc agcgtccttg cttgaaatcg ttcgtaacgt cggctcggcg
      541 gctccgccga accacagtag cacctttccg gccagtttct caatcggtac agccccgtag
      601 cgcctggagt ctatcgaacg ttccgcgtta tcgccgacga cgaaggcctt gcctgccggc
      661 aggtacgccg tgccggcgag cgacgagcgc ggagacgggc ctagggagac gacgcctcct
      721 cgcccgcgca ggctgccggc ctccgctacg atgaaggccg ggccgacgtc gaacacgcgc
      781 ttgatggcct tgcgacgctc gggcgggaac ggcgacacga gcacgatgtc acccggggca
      841 ggctctcccc agcggaccag gtacgatcca gatagcggta ggcggagacc gtaggcgcag
      901 ggcgcgacga cggccacggc gccggagcct atggtcggac tcatcgactc gccgctgact
      961 atgacgaggt ccagggcgaa cgccttgagc gctaccgcga gcgccacggc agcgactatg
     1021 atgcgcgccg ctgagagccg gccccgaggc gatccgcgcc acctatcggg ctcgacctcc
     1081 gtcaacgggc agccccggcc tcaacgccgc gccataatgc gccgatagat aatcgtatgg
     1141 aaatgcagac tgtcatcgcg gatcagtata tagcaagaag gcgaaagccg atacgaatca
     1201 gcgtcgatcg cgggttctcg catagcttgg ccgacggccg catcgccatg aaaccggcca
     1261 ggcgcgagag ccggcccgtg acgctcgagc cgagcgagcc gtcatggagg caatccatcg
     1321 gcagacgcgc caaggctcgg cccaaccagc caggcggtcg ggtcgcaaag cgggtagccg
     1381 tcatcgattc catctccggg ctcgtcgaag cgctcgcttc tccgagggtc gctgcgccga
     1441 tcgccgggat cgccggcgcg atagcgctca tggtcgctat cgcggcggtc ggagccttcg
     1501 ggcgccccgc cgacctcacc tccttcgtcc tacctgacga cggctcgtcc gcggcgatcc
     1561 tactcgacgc gctatcgccg gccgccgact atggggccga ttcgataccg acccccgagt
     1621 tgccgatgac gctcaggata gcgacctata cggtagcgaa gaacgatacg ctcgacgcca
     1681 tttccaggcg atacggccta cggatggaca cgctgatcag cgtcaacggc ctcggcgacg
     1741 cgcgacgcat catctcaggc tcgacgctca aggtgccgaa cctagacggc gtcgtccata
     1801 cggtgacgaa gggcgagtcg ctcgcaggca tcgccgcggc caagggcgtc tccgtgctcg
     1861 atatcgtcga cgcgaacgat ttggccagcc agaccatcct gccgg
//
LOCUS       DAOOLN010000330         1904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_76701_length_1904_cov_2.888588, whole genome shotgun sequence.
ACCESSION   DAOOLN010000330 DAOOLN010000000
VERSION     DAOOLN010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76701_length_1904_cov_2.888588"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1904)
                     /locus_tag="PLW80_05000"
     CDS             complement(<1..>1904)
                     /locus_tag="PLW80_05000"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPB65895.1"
                     /translation="EPAINVDLDGSLDATLERIAGYIAEGAEAFLRREYTVLVPFVAL
                     VALFLAVANSGSLRLEALAFVLGALCSAAAGYFGMRTATAANSRTASAASDGIGPALR
                     VAFAGGSVMGMTVVGLALLGIAAVVGLGVALFGASIGALRDTIFLILSGFSLGASTIA
                     LFARVGGGIFTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDVAGMGADLF
                     ESYVGSIVGCVVLGIGVAASDDMKLRLALLPILMAAMGIVASIAGTFLVRGKPGKSPQ
                     KALNAGSFGAAALAAALMYPLARLALGSGTFDGGRNALSVALAAVFGLVSGTAIGLLT
                     EFFTGTNTAPVRKIERSCDTGAATAIITGLGVGMLSTLPPVLVICGAMLGSYSIAGLY
                     GIGMAALGMLVTLGIQLSVDAYGPIADNAGGLAVMSAMDPHVRDVTDELDAVGNTTAA
                     VGKGFAIGSAALTAIILFSAFAQSAGGSNALVFDVTAPKVLVGLLLGGMIPFLFSALS
                     MDAIGASAFAMIEEVRRQFRERPGILEETETPDYRACVDISTKASLRRMILPGVIAAA
                     SPVVVGFAGGIEMLGGLLAGVTVTGVLLAIFMSNSGGAWDNAKKLIESRAHGKGSDAH
                     KAAVVGDTVG"
BASE COUNT          324 a          639 c          682 g          259 t
ORIGIN      
        1 gccgacggtg tcgccgacga ccgccgcctt gtgggcgtcg gagcccttgc cgtgcgcgcg
       61 gctctcaatg agcttcttgg cgttatccca ggctccgccc gagttcgaca tgaagatggc
      121 caggagcacg ccggtcaccg tgacgccggc cagcaggccg ccgagcatct cgatgccccc
      181 cgcgaagcct accacgaccg ggctggccgc ggcaatgacg ccgggcagta tcatcctgcg
      241 cagcgaggcc ttggtcgata tgtcgacgca ggcgcggtag tcgggggtct cggtctcctc
      301 gagtatgccc ggccgctcgc ggaattgcct gcgcacctcc tctatcatcg cgaaggcgga
      361 ggcgccgatc gcgtccatcg acagcgccga gaacaggaag gggatcatgc cgccgaggag
      421 caggccgacg agcaccttgg gagcggtgac gtcgaacacg agcgcgttgg agccgccggc
      481 ggactgcgcg aaggcgctga acaggatgat ggccgtcagc gcggccgagc cgatcgcgaa
      541 gcctttgccc accgcggccg tcgtgttgcc gacggcgtcg agctcgtcgg tcacgtcgcg
      601 tacgtgggga tccatcgccg acatgacggc caggcccccg gcgttgtcgg cgatcgggcc
      661 gtaggcgtcg acggacagct ggatgccgag cgtgacgagc atgccaaggg ccgccatgcc
      721 gatgccgtag agcccggcga tggagtacga acccagcatg gcgccgcaga tcacgaggac
      781 gggcggcagg gtcgacagca tgccgacgcc gaggccggta atgatcgcgg tggcggcgcc
      841 ggtatcgcat gatcgttcga ttttcctgac cggcgccgtg ttggttccgg tgaagaactc
      901 ggtcaggagg cctatcgccg tgccagagac gaggccgaat accgccgcga gggccacgct
      961 gagcgcgttc ctgcctccat cgaaggtacc ggagccgagc gctaggcgcg ccagcgggta
     1021 cataagcgcg gcggccaggg cggccgctcc gaagctaccc gcgttcagcg ccttctgggg
     1081 cgacttgccc ggcttgccgc gcacgaggaa ggtgccggct atcgacgcga cgatgcccat
     1141 cgccgccatc aggatgggca ggagcgccag gcgtagcttc atgtcgtcgg acgcggccac
     1201 gccgatgccg agcaccacgc agccgacgat ggagccgacg tacgactcga acaggtcggc
     1261 gcccatgcca gccacgtcgc cgacgttgtc gccgacatta tcggctatga ccgcgggatt
     1321 gcgcgggtcg tcttcgggga tgccagcctc caccttgccg accaggtcgg cgccgacgtc
     1381 ggccgccttc gtgaagatgc cgccgccgac acgcgcgaac agcgctatcg tcgaggcgcc
     1441 taggctgaag ccggacagga tcaggaagat cgtgtcgcgg agcgccccaa tggaggcgcc
     1501 gaagagggcg acaccgaggc cgacgacggc cgcgatgccg agtagggcca gtccgaccac
     1561 ggtcatgccc atcaccgagc ccccggcgaa cgccacgcgc agggccgggc cgatgccgtc
     1621 cgaggcggcc gaggccgtcc tcgagttggc ggccgtcgcg gtacgcatgc cgaagtatcc
     1681 ggctgccgcc gagcataggg cgccaagcac gaaggccagg gcctcgagcc tgagcgagcc
     1741 ggagttggcc acggccagga agagcgcgac cagggcgacg aagggcacga gtaccgtgta
     1801 ctcgcgccgc aggaaggcct cggcgccctc ggctatgtat ccggctatcc gctcgagcgt
     1861 cgcgtcgagg gaaccgtcga ggtcgacgtt gatggccggc tcca
//
LOCUS       DAOOLN010000331         1899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_77016_length_1899_cov_2.441974, whole genome shotgun sequence.
ACCESSION   DAOOLN010000331 DAOOLN010000000
VERSION     DAOOLN010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77016_length_1899_cov_2.441974"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..331
                     /locus_tag="PLW80_05005"
     CDS             77..331
                     /locus_tag="PLW80_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPB65896.1"
                     /translation="MECKPGCGACCVAPSVSSPIPGLPNGKPAGVPCPQLGDDYRCAL
                     FGLPERPAVCSSLRPEPEMCGASRAEALAYLESLERETAP"
     gene            386..1027
                     /locus_tag="PLW80_05010"
     CDS             386..1027
                     /locus_tag="PLW80_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HPB65897.1"
                     /translation="MDAIRMWRNDTLGEAAVKALKKNGFDAVYVRTGAEAADLVAKHF
                     KKGASVGFGGSMTVKAIGVQDRAAAAGCELLDHNAPGLAPEAKGAILRRQLTSDVFVS
                     GSNAVTLDGEIVNVDGTGNRVAALSFGPAKTVVVVGINKVVRDLDEAFARIETSAAPM
                     NNKRLDRPNPCVKAGTCMDCDGDTRICRIYQILRKRPGGTDFTVIVVGEDLGY"
     gene            complement(1062..>1899)
                     /locus_tag="PLW80_05015"
     CDS             complement(1062..>1899)
                     /locus_tag="PLW80_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HPB65898.1"
                     /translation="EYLGGAYAVWDRKENAPWRGYELETKAVPVPTPPGAPPSSAAPA
                     MTSVTERRYYGEYWVDPYSEKVWEYNVAIAREIIARGFDEVQFDYIRFPTDGANLGDA
                     SYRWKDPGMDMESALMSFLSYARRNIDAPISIDIYGANGWYRSGVRTGQDVELLARYV
                     DAICPMFYPSHFEQGFMAHAPAELRPYRIYKIGTLRNSYIARKRVVVRPYVQAFYLNV
                     RYDREYYSPAYVALEVDGVRDASNEGLLYWNNSGRYDDIPTLKRGADRRIVSKPERSV
                     LD"
BASE COUNT          320 a          618 c          638 g          323 t
ORIGIN      
        1 gactcaaaat ctgctgcccg cgagggcgtg agggttcgag cccctctgcc ggtatcttct
       61 ccgaacggga gcgaccatgg aatgcaagcc aggttgcggc gcgtgttgcg tcgccccgtc
      121 cgtatcgtcg cccataccgg gcctcccgaa cggtaagccg gccggcgtcc cctgccccca
      181 gcttggcgac gactaccgct gcgcgctgtt cggcctaccg gagcgacccg cggtctgctc
      241 gtcgctacgg cccgagcccg agatgtgcgg cgcctcgcgc gccgaagcgc tcgcgtacct
      301 cgaatcgctc gagcgagaaa cggctcctta gggtataatc ggattgtagt gctcatccct
      361 actatatcga acccaggagg atgctatgga cgccatcagg atgtggagga acgatacgct
      421 cggcgaggcg gccgtcaagg cgctcaagaa gaacggcttc gacgcggtat acgttagaac
      481 cggtgccgag gcggccgacc tcgtcgccaa gcatttcaag aagggcgcga gcgtcggctt
      541 cggcggctcg atgaccgtca aggccatcgg cgtacaggac cgggccgcgg cggccggctg
      601 cgaactcctg gatcataacg cccccgggct ggcgcccgag gccaagggcg ccatacttcg
      661 gcgccaactc acgagcgacg tcttcgtctc cgggtcgaat gccgtgacgc tcgacggcga
      721 gatagtcaac gtcgacggta cgggcaaccg cgtggcggcg ctcagcttcg gtccggccaa
      781 gacggtcgtg gtggtcggga tcaacaaggt cgtccgcgac ctcgacgaag ccttcgcccg
      841 catcgagacc agcgccgccc cgatgaacaa caagcggctc gaccggccga acccctgcgt
      901 caaggcgggc acctgcatgg actgcgacgg cgacacgagg atctgccgga tctaccagat
      961 actgcgcaag cggccgggcg gtaccgactt cacggtcatc gtcgtcggcg aggacctggg
     1021 gtactaggat gggccgggcg gggccgcgag tcggctccgc ctcagtccag cacgctccgt
     1081 tccggcttgg agacgatgcg ccggtcggcg ccccgcttca gcgtcggtat atcgtcgtac
     1141 cttccggagt tattccagta caacaggccc tcgttcgacg cgtcgcgtac gccgtccacc
     1201 tcgagcgcca cgtaggccgg tgagtagtac tcgcggtcgt agcgcacgtt caggtagaag
     1261 gcctgcacat agggccggac gaccacgcgc ttcctggcga tgtacgagtt gcgtagcgtg
     1321 cctatcttat agatgcggta ggggcgtagc tccgccggcg cgtgggccat gaagccctgc
     1381 tcgaagtgcg agggatagaa catcgggcag atcgcgtcga cgtagcgggc gagcagctcg
     1441 acgtcctggc cggtgcggac gccggagcgg taccagccgt tggcgccgta gatgtcgatg
     1501 ctgatcggcg cgtctatgtt gcgcctggcg taggacagga aggacatgag cgcgctctcc
     1561 atgtccatgc ccgggtcctt ccagcggtag ctggcgtcgc cgaggttggc gccatcggtc
     1621 gggaagcgta tgtaatcgaa ctggacctcg tcgaagcccc gggcgataat ctcccgggct
     1681 atggctacgt tgtactccca gaccttctcg gagtacggat cgacccagta ttcgccgtaa
     1741 tacctgcgct cggtgacgct ggtcatcgcc ggcgccgcgg aggacggcgg ggccccaggc
     1801 ggcgtcggta ccgggaccgc cttcgtttca agctcgtagc cgcgccaggg ggcgttttcc
     1861 ttcctgtccc agaccgcgta ggcgccgccc aggtactcg
//
LOCUS       DAOOLN010000332         1893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_77373_length_1893_cov_2.651251, whole genome shotgun sequence.
ACCESSION   DAOOLN010000332 DAOOLN010000000
VERSION     DAOOLN010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77373_length_1893_cov_2.651251"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PLW80_05020"
     CDS             <1..481
                     /locus_tag="PLW80_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65899.1"
                     /translation="AMAAAYGSSLPHGIGTFVYTSRRDKYTWRAAEPAKVRALASSVL
                     KAGRPSLAVGAAHGSIMAGLFLAELLGCELWFLRFSMFKRNDTAPIVSAHDERKLRAH
                     GDGSDILVFDEDSASGATLSLLSARVKEMAPEARTGAVIRHQSAAFRPDHVGRVWWD"
     gene            complement(492..>1893)
                     /locus_tag="PLW80_05025"
     CDS             complement(492..>1893)
                     /locus_tag="PLW80_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPB65900.1"
                     /translation="GIWLGITALGLILSKRIAAVLKQFKHASAFSIMALGLAFLLSGF
                     FEREGLAMIIGAYITGLSLSKTDISHIIIEKVHVLYEFFVPVFFAVMGMMVDLRQLAS
                     LEVILIGLVFTVISILAKVLGCGLPALFTGFNLKGALRIGAGMVPRGEVALIVAGIGV
                     TNGLLDGGLFGIAIMMTLLTTLVAPFGLSATLRIPGAGTRDPEASSESKAVDFDFPSE
                     DVALLVTDTMAHELQAEGYYVRTMDIEEGIAQVRRDELAFSMRLDGARLSVQAPEKTL
                     AIVRTSVFEAVATLNQNFARLKSDFDPASIQRNDSGEVGPLGRSEAKAVCPSCVALEL
                     KADTKEGAIRELLELMAGNGLIDDTERAFADILERERSMSTGMQHGVALPHAKTDAVS
                     AMAASIGIKRDGLEFDSLDGQPARIIVLLTTPKKNPGAHIQFLAGVGAALKDERRRGQ
                     ILEATSAEDVIRLMGV"
BASE COUNT          342 a          617 c          617 g          317 t
ORIGIN      
        1 cgccatggcg gccgcctacg gctcgagcct gccgcatggg atagggacct tcgtctacac
       61 ctccaggcgc gataagtaca cctggagggc cgcggaaccg gcgaaggtca gggcgctggc
      121 cagctcggtg ctcaaagccg gaaggccgag cctggcggtc ggggccgccc acggatccat
      181 catggcgggg ctcttcctgg ccgagctcct cggctgcgag ttatggtttc tgcgcttcag
      241 catgttcaag cgcaacgaca cggcgccgat cgtctcggcc cacgacgagc ggaagctccg
      301 cgcccacggg gacggatcgg acatactggt gttcgacgag gactccgcgt ccggcgcgac
      361 cctgtcgctg ctatcggctc gcgtcaagga gatggcgccg gaagccagga ccggggcggt
      421 catacggcac cagagcgccg cgttccgccc cgaccacgtg ggccgggtat ggtgggactg
      481 acggcccgcc cttagacgcc catcagccgg atgacgtcct cggcgctcgt ggcctcgagt
      541 atctgccccc tgcgccgctc gtccttcagc gcggccccga cgcctgcgag gaactggata
      601 tgggcgccag ggttcttctt aggcgtcgtc agaagcacga tgatcctggc cggttgccca
      661 tcgagcgagt cgaactcaag cccgtcccgc ttaatgccta tggaagccgc catagcgctg
      721 accgcgtcgg tcttggcgtg cggcagggcg acgccatgct gcataccggt gctcatcgag
      781 cgctcgcgct cgagaatgtc ggcgaaggcc cgctccgtat cgtcgatcag cccgttgccg
      841 gccatcagct cgagcagctc gcgtatggcc ccttccttgg tatccgcctt cagttcgagc
      901 gcgacgcagg acgggcagac cgccttggct tccgaccggc cgagggggcc gacctcgccc
      961 gagtcgttcc gctggatgct cgccgggtcg aaatccgatt tgagcctggc gaaattctgg
     1021 ttgagggtag ctacggcctc gaagaccgag gtcctgacga tagccaacgt cttctcgggg
     1081 gcctggacgc tcagtcgcgc cccatccagc cgcatcgaaa aggccagctc gtcgcgcctg
     1141 acctgggcga tgccctcctc gatatccatg gtcctcacgt agtagccctc ggcctgcagc
     1201 tcgtgagcca tcgtatcggt gacgagcagg gcgacgtcct cggacggaaa atcgaagtcg
     1261 acagccttcg actcgctgga agcctccgga tccctcgtac ccgccccggg gatgcgaagc
     1321 gtcgccgata gcccgaacgg cgcgaccagg gtcgtcagca gcgtcatcat gatggcgatg
     1381 ccgaagagtc cgccatcgag gaggccgttg gtaacgccga tgccggctac gatcagcgct
     1441 acctcgccgc gcggaaccat gccggcgcct atcctgagcg cccccttgag gttaaaaccg
     1501 gtgaacaggg ccggcaggcc gcagcccagc accttggcta ggatgctgat gacggtgaag
     1561 acgagcccaa tgaggataac ctcgagcgat gcgagctggc gcaggtcgac catcatgccc
     1621 attaccgcga agaagaccgg tacgaaaaac tcgtagagca cgtggacctt ctcgatgatg
     1681 atgtggctga tatcggtctt cgatagagac aagccggtta tgtaggcgcc gatgatcatc
     1741 gccaggccct cccgctcgaa gaagcccgag aggaggaagg ctaggccgag agccatgatg
     1801 gagaaggcgc tcgcgtgctt gaactgcttg agtacggcgg ctatacgctt ggacaggatc
     1861 agcccgagcg ccgtgatacc gagccagatg ccg
//
LOCUS       DAOOLN010000333         1889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_77592_length_1889_cov_3.070883, whole genome shotgun sequence.
ACCESSION   DAOOLN010000333 DAOOLN010000000
VERSION     DAOOLN010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77592_length_1889_cov_3.070883"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PLW80_05030"
     CDS             complement(<1..204)
                     /locus_tag="PLW80_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65901.1"
                     /translation="MNANGTNGLSPAVRRRRFAVRAGLVAQYFVVGAWVMLSGREHTL
                     LLDNKALADGSAPAFRRVKVYVDG"
     gene            complement(201..1322)
                     /locus_tag="PLW80_05035"
     CDS             complement(201..1322)
                     /locus_tag="PLW80_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB65902.1"
                     /translation="MTKRFDFKRFVADNVVVVIFLVLTAIATPISGLSANYIIQDIIT
                     RIGRNSFLVFSLLLPVMAGMGLNFGMVLGAMAGEIGLIFAVDWGISGVWGLGFASLVG
                     LPIAVALGWLCGSILNRAKGREMATGYIIGFFMDGVYQFAVLYLMGSLIPMRSLAITL
                     SRGYGIRNTLNLDSVRQSLDNLAMVKVFGFNVPVATYLVVAALCVFIVWFRKTKLGQD
                     MRAIGQDQGVSNASGIPVEKTRIIAIVVSTVLAMVGQIIFLQNMGNMATYNAHRQTGF
                     FAVAAILVGGASVTKATIPNVFVGVVLLHLMYIVMPRAGAELFGSAQIGEYFRDAFSY
                     GAIALALVLHAWRKRRGVEIDRAGLRRNTVSPDAKGGKA"
     gene            complement(1319..>1889)
                     /locus_tag="PLW80_05040"
     CDS             complement(1319..>1889)
                     /locus_tag="PLW80_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB65903.1"
                     /translation="GGAGLRTTITLDNYWSKILNDFLVVRIGGSFVFPTGMILFFALC
                     AFFVYLFFRTKTGTAMTAVGSNPDFARAGGVSADKMRTVSVMLSTVLGAVGILVYQQS
                     FGFIQLYQAPLYMAFPAVAALLIGGASVNKASMVNVVVGTLLFQGILTMTPSVINSIL
                     QTDMSEVIRIVLSNGMILYALTRKTKVSK"
BASE COUNT          357 a          600 c          627 g          305 t
ORIGIN      
        1 cccgtcgacg tagaccttga cgcgcctgaa cgccggcgcc gagccgtcgg ccagggcctt
       61 gttgtccagg agcagcgtat gctcgcggcc gctgagcatc acccaggcgc cgacgacgaa
      121 atactgcgcg accaggccgg cgcgtacggc gaagcgcctg cgcctgacgg ccggcgatag
      181 accgttggtt ccgttggcgt tcatgccttg cctcccttgg cgtccgggct gaccgtgttc
      241 cggcgcaggc ccgcccggtc gatctcgacg ccgcggcgct tgcgccaggc gtgcagcacg
      301 agggccagcg ctatcgcgcc gtagctgaat gcgtcgcgga agtactcgcc tatctgggcc
      361 gagccgaaca gctcggcgcc cgcgcgcggc atcacgatgt acatcaggtg caggagcacg
      421 acgccgacga acacgttcgg gatcgtagcc ttggtcaccg aggcgccgcc gacgaggatg
      481 gccgccacgg cgaagaagcc ggtctgccgg tgcgcgttgt aggtggccat gttgcccatg
      541 ttctggagga agatgatctg gccgaccatc gctaggaccg tcgagacaac gatggcgatg
      601 atgcgcgtct tctccaccgg gatgcccgag gcgttggaaa cgccctggtc ctggccgatg
      661 gcgcgcatgt cctggccgag cttggtcttc ctgaaccaga cgatgaacac gcagagcgcc
      721 gcgaccacga ggtaggtggc gaccggcacg ttgaagccga agaccttgac catcgccagg
      781 ttgtccaggc tctgcctgac cgagtccagg ttcagggtgt tacggatgcc gtagccgcgc
      841 gacagcgtga tggcgagcga gcgcatcggt atcagcgagc ccatcaggta cagcaccgcg
      901 aactggtaga cgccgtccat gaagaagccg atgatgtagc cggtagccat ctcgcggccc
      961 ttggcccggt tcaggatgga gccgcatagc cagccgaggg ccacggcgat cggcaggccg
     1021 acgagcgagg cgaagccgag gccccagacg ccggaaatgc cccagtcgac cgcgaagatc
     1081 aggccgatct ctccggccat cgcgccgagc accatgccga agttcaggcc catgcccgcc
     1141 atgaccggca ggagcaggct gaacaccagg aacgagttgc gcccgatgcg ggtgatgatg
     1201 tcctggatga tatagttcgc cgacagcccc gagatcgggg tcgcgatggc cgtaagcacg
     1261 aggaagatga cgacgaccac gttgtcggcg acgaacctct tgaaatcgaa ccgcttcgtc
     1321 atttcgacac cttcgtcttt ctggtcagcg cgtacaggat catgccgttc gacagcacga
     1381 tgcgtatcac ctccgacatg tccgtctgca ggatgctgtt gatgacgctc ggcgtcatcg
     1441 ttaggatgcc ctggaacagc agggtgccga ctaccacgtt gaccatcgag gccttattga
     1501 cgctggcgcc gcctatcagg agggcggcca cggccgggaa ggccatgtac agcggcgcct
     1561 ggtacagctg gatgaagccg aagctctgct ggtacacgag gataccgacc gcgccgagca
     1621 ccgtcgacag catcacgctg acggtgcgca tcttgtcggc cgacacgccg ccggccctcg
     1681 cgaagtccgg gttcgagccc acggcggtca tcgcggtgcc ggtcttggtc ctgaagaaca
     1741 ggtagacgaa gaaggcgcac agcgcgaaga acaggatcat gccggtcggg aacacgaagc
     1801 tgccgcctat cctgaccacg aggaagtcat tgaggatctt gctccagtag ttgtcgagcg
     1861 tgatcgtcgt cctgaggccg gcgccgccg
//
LOCUS       DAOOLN010000334         1887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_77718_length_1887_cov_2.766376, whole genome shotgun sequence.
ACCESSION   DAOOLN010000334 DAOOLN010000000
VERSION     DAOOLN010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77718_length_1887_cov_2.766376"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1857)
                     /locus_tag="PLW80_05045"
     CDS             complement(<1..1857)
                     /locus_tag="PLW80_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="signal protein"
                     /protein_id="HPB65904.1"
                     /translation="MALETLTRAAFDANEDGDWFFSIRAQDYAGNWSDASRVRFVRDT
                     TPPGKPAIAPPAASADGFVASSSFALAWEPPADLDVAGYSWALEYLGPLDRPPARKRP
                     APATGAARPGRAYDLWPTTDYERLLWTSAEPAPPAPSLRTRSPTAAFSNMDDGYWIFS
                     VAAIDGVGNVGEPARALLRTDKFRPYTAVTDVSSRRDDFGNLELAVVGRGFLDDGAVT
                     RLAVDADGREPFDYLYVLADREYSIASDRLLRVPEVVDLRAGSYRIGLYHPERGWYFT
                     DPRLSVDYSGTVKFGERGAPWSPTWSFAPAPSSLVSVSTLFTLAALLLPSLGIVLSLR
                     HVVVLAGQMREARAEAIALLEGKPMPESERKRKAKRAIRRGAGLTAKFAMTISLLVLF
                     IVALVSIRLGIQMLQTQSETLARGLEQRARVLLESAVQGGKSYLPAKNVLELSLLPNQ
                     ASAISEARYLTITGFGASGATNPDVVWASNDPDIASKIDSQALVPGVSLLDDTLSPRV
                     SSMSAEIDARANAEVGAIAEALQKMQDEGRALAANLDAASQDGLAEIASSARVMEQEL
                     SERLAAIADASVASEPRFDATKLGATAEEYVFYKPILFRQGREALDYRGLVRL"
BASE COUNT          282 a          620 c          691 g          294 t
ORIGIN      
        1 cagcctgacg aggccgcggt agtccagcgc ctcgcggccc tgcctgaaca ggatgggctt
       61 atagaagacg tactcctcgg ccgtcgcgcc gagcttggtg gcgtcgaagc gaggctcgga
      121 ggcgaccgag gcgtcggcta tcgccgcgag gcgctcgctc agctcctgct ccatcaccct
      181 ggcgctcgag gctatctccg cgagcccgtc ctggctggcc gcgtcgaggt tggccgccaa
      241 ggcgcggccc tcgtcctgca tcttttgcag ggcctcggct atcgcgccga cctcggcgtt
      301 ggcccgcgcg tcgatctcgg ctgacatgga ggaaacccgc ggcgatagcg tgtcgtcgag
      361 cagggagacg ccaggcacca gcgcctgact gtcgatcttg gaggcgatgt ccgggtcgtt
      421 gctcgcccag acgacgtcgg ggttcgtggc gcccgaggct ccgaagccgg ttatggtcag
      481 gtagcgcgcc tcggagatgg cgctcgcctg gttcggcagc agcgacagct cgagcacgtt
      541 cttggcggga aggtacgact tgccgccctg gacggcgctc tcaagcagta cccgagcccg
      601 ctgctcgagg cctcgagcca gcgtctcgct ctgcgtctgg agcatctgga tgccgagtcg
      661 tatcgacacg agggcgacga tgaacagcac gagcaggctg atcgtcatcg cgaacttggc
      721 ggtcaggccg gctcccctac ggatggcccg tttggccttc cgcttgcgtt ccgattcggg
      781 catgggcttt ccctccagta gcgctatcgc ctcggctcgc gcctctctca tctggcccgc
      841 cagcacgacg acgtgcctga gcgacaggac gataccgaga gacggcagaa gcaaggcggc
      901 tagcgtgaac agcgtggata cgctgactaa cgacgacggg gcgggggcga acgaccaggt
      961 cggggaccag ggcgcgccgc gctcgccgaa tttcacggtg cctgagtaat cgaccgacag
     1021 gcgcggatcg gtgaagtacc agccgcgctc cggatggtag aggccgatac ggtacgagcc
     1081 ggcccgcagg tcgacgacct cggggacgcg tagcaggcga tccgaggcta tcgagtactc
     1141 gcggtcggcc agcacgtaga ggtagtcgaa gggctcgcgc ccatcggcgt cgacggccag
     1201 cctggtcacg gccccgtcgt ccaggaagcc tcggccgacg acggccagtt ccaggttgcc
     1261 gaagtcgtcg cgcctggagc tgacgtcggt gacggccgtg tacggcctga acttgtccgt
     1321 tcggagtagc gcccgcgcgg gctcgccgac gttgccgacg ccgtctatcg cggccaccga
     1381 gaagatccag tagccgtcgt ccatgttcga gaaggctgcc gtcggcgaac gcgtgcgcag
     1441 tgacggggcc ggcggcgcgg gctcggccga ggtccagagc aggcgctcgt agtcggtggt
     1501 cggccagagg tcgtaggctc ggcccgggcg cgccgcgccg gtagcgggcg ctgggcgctt
     1561 gcgggctggc gggcggtcga gcgggccgag gtactcgagg gcccaggaat agccggcgac
     1621 gtccagatcg gccgggggct cccaggcgag cgcgaagctg ctcgacgcga cgaagccgtc
     1681 ggccgaggcg gccggaggcg cgatcgccgg cttgcccggc ggcgttgtgt cgcgtacgaa
     1741 gcgcactcgc gacgcgtccg accagttgcc ggcgtagtcc tgcgccctga tcgagaagaa
     1801 ccagtcgccg tcctcgttcg cgtcgaaggc ggcgcgggtc agcgtctcga gcgccatgac
     1861 cgtctcgggc ggttcggcct cgggatc
//
LOCUS       DAOOLN010000335         1886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_77766_length_1886_cov_4.018023, whole genome shotgun sequence.
ACCESSION   DAOOLN010000335 DAOOLN010000000
VERSION     DAOOLN010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77766_length_1886_cov_4.018023"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..935)
                     /locus_tag="PLW80_05050"
     CDS             complement(24..935)
                     /locus_tag="PLW80_05050"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPB65905.1"
                     /translation="MKTHRATTKWLILAVMGALAIAWLIIGLWPRSGMAAPPLWRAGA
                     DGAPLVLAHQGGAMERPSSSDLAFERASEIGVDAFELDVVLTADGVLAVIHDTTLDRT
                     TDGVGPVGDRTYAEIRFLDAGYGLLGEDGEVVRDPARNPYIGIGAYVPSLEELFREYP
                     SMPMLIEIKDSSDRGLASARELRRLIDAYGRATTTMVACNDEEPLLEFDSLPGERVAR
                     AASKPEAYRFYARHALGLNALNNRAPHEAMSLSLTSGLGPFTIDLTARRLRRDCVARG
                     LSTMYWTVNDGPTMERLLSTAADSGFS"
     gene            complement(1023..>1886)
                     /locus_tag="PLW80_05055"
     CDS             complement(1023..>1886)
                     /locus_tag="PLW80_05055"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HPB65906.1"
                     /translation="AVILFKPFLALVNLMAGGSTVAQHIAMMHTVFNVINAAVFAPFA
                     KQLSELVTRFVKAKPGEDDLPQKLEYVAAPLVDSPELNLIRAQKEISDMAGLCGSMFV
                     RWRELLRDGTEDVSDEVERFRSLEDYADQMRDELSRFLLECAGHEVSADRQRDIGKMM
                     RIVVDLEDITDDCFSLVLLLDKARSRGLVVDAEEIASLGPYTLLVENFLRFVKDNING
                     AISAEQLAIAQDLENSIDDFRTTLKKKARKRLKSGENVKAELLFMDMVRHIEKIGDHA
                     FGVAHALRTRS"
BASE COUNT          313 a          618 c          604 g          351 t
ORIGIN      
        1 tatgtcaaag gccagaagct cacttatgag aatcccgaat ccgccgccgt cgatagcagg
       61 cgctccatcg tcggcccgtc gttgaccgtc cagtacatcg tcgataggcc ccgggcgacg
      121 cagtcgcggc gaagccgcct cgccgtcagg tcgatcgtga atgggccgag cccgctcgtc
      181 agcgacaggc tcatcgcctc gtgcggagcc cggttgttga gcgcgttcag cccgagcgcg
      241 tgccgggcgt agaagcggta cgcctcgggc ttggacgcgg cccgagcgac ccgttcaccg
      301 ggtagcgagt cgaactccag gaggggttcc tcgtcgttgc aggcgaccat cgtggtggtc
      361 gcgcgcccgt aggcgtctat cagccgcctg agctccctcg cagaggccaa gccccggtcg
      421 ctcgagtcct ttatttctat cagcattggc atggaggggt actcgcggaa cagctcctcg
      481 agcgacggca cgtaggcccc gatgcctatg taggggttcc tggcagggtc gcgcacgacc
      541 tcgccgtcct cacccaggag gccgtagccg gcgtcgagga agcgtatctc ggcgtaggtt
      601 ctgtcaccga ccggcccgac gccgtcggtc gtacggtcga gggtcgtatc gtggataacc
      661 gccaggacgc cgtcggccgt gagtaccaca tcgagctcga acgcgtcgac gccaatctcg
      721 gaggcgcgct cgaaggccag gtcgctcgac gacggccgct ccatcgctcc gccctgatgc
      781 gccagcacga gcggagcgcc gtcggcgccg gcccgccaga gtggtggcgc ggccatgccg
      841 gacctgggcc acaggccgat gatcagccac gcgatggcga gcgcgcccat caccgcgagg
      901 atgagccatt tcgtcgtagc tctatgcgtt ttcatgcgga cagtatacga ccgaaccggc
      961 aacccgcaag cgcgatcgtc gctctcgatg cgatcgtcgc tcgcgatgcg atcgtcgctc
     1021 gcctagctcc tggtcctcag ggcgtgggcc acgccgaacg catggtcgcc tatcttctct
     1081 atgtgcctaa ccatgtccat gaataggagc tcggccttca cgttctcgcc ggatttgagc
     1141 cgcttgcgcg ccttcttctt gagcgtcgta cggaagtcgt cgatactgtt ctccaggtcc
     1201 tgggctatgg cgagctgctc cgcgctgata gcgccgttga tgttgtcctt gacgaagcgc
     1261 aggaagttct cgacgagcag agtatagggc cccaggctcg ctatctcctc cgcgtcgacg
     1321 acgagcccgc gcgagcgggc cttgtccagc aacagcacga ggctgaaaca gtcatcggtg
     1381 atgtcctcca ggtcgacgac gatgcgcatc atcttgccaa tgtcgcgctg tctgtcggcg
     1441 ctcacctcgt gtccggcgca ctcgagtagg aatctcgaca gctcgtcgcg catctggtcg
     1501 gcgtagtcct ccaggctgcg gaagcgctcc acctcgtcgg agacatcctc ggtcccatcg
     1561 cgcagcagct cgcgccaacg gacgaacatc gagccgcata ggccggccat gtccgagatc
     1621 tccttctgcg cccggatcag gttgagctcc gggctatcga cgagcggggc ggccacgtac
     1681 tcgagtttct gcggcaggtc gtcctcgccg ggcttggcct tgacgaacct ggttaccagt
     1741 tcggacagtt gcttggcgaa gggcgcgaag accgccgcgt tgatcacgtt gaagaccgta
     1801 tgcatcatcg caatgtgctg ggccacggtg gagcccccgg ccatcaggtt gacgagggct
     1861 aggaatggct tgaataggat cacggc
//
LOCUS       DAOOLN010000336         1886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_77773_length_1886_cov_3.405243, whole genome shotgun sequence.
ACCESSION   DAOOLN010000336 DAOOLN010000000
VERSION     DAOOLN010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77773_length_1886_cov_3.405243"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PLW80_05060"
     CDS             complement(<1..400)
                     /locus_tag="PLW80_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB65907.1"
                     /translation="MNAFIRVVDAVKTFGDPERGGVRALDGVSLEVAKGEVVLVIGPS
                     GSGKSTLLRAVNRLERLDSGGIWIGDDEVTARRADLRRIREDAGMVFQSFNLFSHMSV
                     IDNVTLAPRLVRGTPAREALEAGRAILERVG"
     gene            complement(397..1215)
                     /locus_tag="PLW80_05065"
     CDS             complement(397..1215)
                     /locus_tag="PLW80_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPB65908.1"
                     /translation="MTNGRRAARIAAGVPWWLVLLVGLGAIALGTALADASYRVIFGA
                     VARGFGVTIGVALASYAASLALGLLLGLARSSRLRPLREASTFYIELVRGLPMLVILY
                     YIAFVGAPALAEVLNGLLAPLIRAGAVKAFQARDFSFATRAGLALTLGYAAFIAEIFR
                     AGIESVDKGQIEAAHALGLSHSQTMLRVVLPQAVKNVLPALANEFVAIIKDSALVSVL
                     GVQDITQIGKVYAASTFKFFETYNVVAFFYLCMTVSLSMLVRALERRLKRENAR"
     gene            complement(1244..>1886)
                     /locus_tag="PLW80_05070"
     CDS             complement(1244..>1886)
                     /locus_tag="PLW80_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPB65909.1"
                     /translation="KTGKAMGWEYDAVNEIARRLNAKVDWQLSSWDLMIQSVRDGQFD
                     VGMDGITITDERKKQVDFSDPYMVSQQFMLVRADEKRFSDAAAFAADPKLLVGAQAGT
                     TNFYVAVYEVLDGDEQNKRIKLFETFGATVQALIAGDVDMVLMDAASSKGYVGANPGK
                     LKSVGGPLGTEEFGFIFPPGSDLVGPFNAALASMKADGTLDRLNTKWFFEYQP"
BASE COUNT          314 a          644 c          623 g          305 t
ORIGIN      
        1 ggccgacgcg ctcgagtatg gcccggcccg cctcgagcgc ctcgcgcgcc ggcgtcccgc
       61 gcacgaggcg cggcgccagc gtcacgttgt cgatcacgct catgtgcgag aacaggttga
      121 agctctggaa gaccatgccg gcgtcctcgc ggatgcgcct gagatcggcc cgccgcgccg
      181 tcacctcgtc gtcgcctatc cagataccgc ctgagtcgag ccgctcgagc cggttgaccg
      241 cgcgtagcag cgtgctctta cccgatccag aggggccgat cacgagcacc acctctccct
      301 tggccacctc gaggctcacg ccatcgagag cgcgcacgcc gccgcgctcg ggatcgccga
      361 aggtcttgac cgcgtctacg actcgtatga aggcgttcat cgggcgttct cccgcttgag
      421 ccggcgctcg agggcgcgta ccagcatgga caggctcacg gtcatgcaga ggtagaagaa
      481 ggccacgacg ttgtaggtct cgaaaaactt gaaggtgctc gcggcgtaga ccttgccgat
      541 ctgggtgatg tcctgcacgc cgagtaccga caccagggcc gagtccttga tgatagcgac
      601 gaactcgttg gccagcgccg gcagcacgtt cttgaccgcc tgcggcagca cgacgcgcag
      661 catcgtctgc gagtgcgaca gcccgagcgc gtgggccgcc tctatctggc ccttgtcgac
      721 gctctcgatg ccggcgcgga agatctcagc gatgaaggcc gcgtagccga gggtcagcgc
      781 gagcccggcc ctcgtcgcga agctgaagtc gcgggcctgg aaggccttga cggcgccggc
      841 tcggatgaga ggcgccagca gcccgttcag gacctcggcc agggccgggg ccccgacgaa
      901 ggcaatgtag tagaggatga cgagcatcgg taggccgcgc accagctcga tatagaacgt
      961 cgacgcctcg cgcagggggc gcaggcgcga cgatcgggcc agcccgagca ggagcccgag
     1021 cgccagcgac gccgcgtacg acgcgagcgc cacgcctatc gtcacgccga agcccctggc
     1081 cacggcgccg aatatgaccc ggtacgacgc gtccgccagc gccgtgccga gcgctatggc
     1141 gccgaggccg acgagcagga cgagccacca cggcacgccc gccgctatcc tggcggctct
     1201 cctcccgttc gtcatcctcc gtccccctac gctccgcccg gtattatggc tggtactcga
     1261 agaaccactt ggtgttcagc cgatcgagcg tgccgtcggc cttcatcgag gctaaggcgg
     1321 cgttgaacgg gccgaccagg tcagatcccg gcgggaagat gaagccgaat tcctcggtcc
     1381 cgagcgggcc gccgacgctc ttgagcttgc ctgggttggc cccgacgtag cccttgctgg
     1441 aggccgcgtc catcagcacc atgtcgacgt cgccggcaat cagcgcctgc acggtggcgc
     1501 cgaaggtctc gaacagcttg atgcgcttgt tctgctcgtc gccgtcgagc acctcgtaga
     1561 cggctacgta gaagttggtg gtgccggcct gggcgccgac cagcagcttg gggtcggcgg
     1621 cgaaggccgc ggcgtcggag aagcgcttct cgtcggcgcg cacgagcatg aactgctgcg
     1681 agaccatgta cgggtcggag aaatctacct gcttcttacg ctcgtcggtg atggtgatac
     1741 cgtccatgcc cacgtcgaac tggccgtcac ggaccgactg gatcatcagg tcccagctcg
     1801 atagctgcca gtcgaccttg gcgttgagac gcctggcaat ctcgttgacg gcgtcgtact
     1861 cccagcccat cgccttgccg gtcttg
//
LOCUS       DAOOLN010000337         1882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_77992_length_1882_cov_3.061850, whole genome shotgun sequence.
ACCESSION   DAOOLN010000337 DAOOLN010000000
VERSION     DAOOLN010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77992_length_1882_cov_3.061850"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1797)
                     /locus_tag="PLW80_05075"
     CDS             complement(<1..1797)
                     /locus_tag="PLW80_05075"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPB65910.1"
                     /translation="MKGNAMLTPTSASGRKAILGAVLVALFALPDRVSADDLMLASTP
                     VFVGREAFEARVAARSGGREPVGLVLSGGSARAFAHIGVLRRLEEDGVVPDYIVANSM
                     GSIIGLLYAAGLSPDQIYGLVSGTELGALFEPTLPVSGGVLDPRRFSDLIRLYLGDLR
                     LEDLPIPIVVICEDLRTKREIRLAEGDLVTILEAAYALPVFFPPVELDGYLLIDGGVT
                     NLVPLGAAREFSDTVIVSSTFYDNPRLNLRNPIIVLNTSIDIGKRRAGVADLLEHPDA
                     LWIRCSVESFSFMSFDRLAELDAEGYRSADAMSDRLAGVESGGVDEALATIRLARGAA
                     IAEAARAWAPFERAPATRPALSVTPTVRSSAYPGDPYLYLDSVFVGAGLGLRWGAFEA
                     AIDAGGDWRAYGDGSFVPAATVSASVDPLPWLRLETLVQADWESLSEHGDDGERVGGV
                     SIPALYQRSALRAALVRGGSRLELSSAVEADGLASEGAAWLLTSGARYVASRPASSKA
                     AAGGALRELSASLGHQLAGDRDEHLAWADASAALSPFASSAALRPLTARLGAMARVAL
                     SAGAAAPFYLSDAVFAPEPAALDGVGPAVYGAS"
BASE COUNT          295 a          660 c          700 g          227 t
ORIGIN      
        1 cgacgccccg tagaccgctg gcccgacgcc gtcgagggcg gccggctcgg gcgcgaacac
       61 ggcgtccgaa aggtagaagg gcgcggcggc gccggcgctc agcgcgaccc gcgccatggc
      121 gccgagcctc gcggtcagcg ggcggagcgc tgccgacgac gcgaacggcg atagggccgc
      181 ggaggcgtcg gcccaggcca ggtgctcgtc ccggtcgcca gccaactgat ggccgaggct
      241 cgcggacagc tcccggagcg cgccgcccgc ggccgcctta ctggacgcgg gacgcgacgc
      301 gacgtacctg gcgccgctgg tcaggagcca ggccgctcct tcggacgcca gcccgtcggc
      361 ctcgaccgcc gaggacagct cgaggcgaga gccgccccgg acgagcgcgg cccggagcgc
      421 gctccgctgg tacagcgccg gtatcgagac gccgcctacc cgttcgccgt cgtcgccgtg
      481 ctcggagagg ctttcccagt cggcctggac gagcgtctcg agcctgagcc acggcagggg
      541 gtcgacgctg gccgagacgg tagcggccgg cacgaacgaa ccgtcgccgt aggcccgcca
      601 gtcgccgccc gcgtcgatcg cggcctcgaa cgcgccccag cgcaggccga gcccggcgcc
      661 gacgaacacg gagtccaggt acaggtatgg gtcgcccggg taggcgctcg agcggacggt
      721 cggcgtgacg gacagggccg gcctcgtcgc cggcgcgcgc tcgaacggcg cccaggcccg
      781 ggccgcctcg gcgatcgccg ctcccctcgc cagccgtatc gtcgccagcg cctcgtcgac
      841 cccgcccgac tcgacgccgg cgagccgatc cgacatagcg tctgcggagc ggtagccctc
      901 ggcgtcgagc tcggccagcc ggtcgaacga catgaacgag aacgactcca ccgagcagcg
      961 tatccagagc gcgtcggggt gctcgagcag gtcggccacg ccggcccggc gcttgccgat
     1021 atcgatggat gtattgagca cgatgatggg gttgcgcagg ttgagccgcg ggttgtcgta
     1081 gaaggtgctc gagacgatga cggtatcaga gaattccctc gcggcgccga gcggcaccag
     1141 gttcgtcacg ccgccgtcga tcagcaggta gccgtcaagc tcgaccggcg ggaagaacac
     1201 cggtagcgcg taggccgcct cgaggatcgt gaccaggtcg ccctcggcca ggcgtatctc
     1261 gcgcttggtc cggaggtcct cgcagatgac gacgatgggg atgggcaggt cctcgagcct
     1321 caggtcgccg aggtagagcc ggatcaggtc gctgaagcgc ctcgggtcga gcacgccgcc
     1381 ggagaccggc agggtcggct cgaacagggc gccgagctcg gtgcccgata ccaggccgta
     1441 gatctggtcg ggcgagaggc cggccgcgta gaggaggccg atgatggagc ccatggagtt
     1501 ggcgacgatg tagtccggca cgacgccgtc ctcctcgagc cgcctgagca cgccgatatg
     1561 ggcgaaggcc ctggccgagc cgcccgacag cacgagccct accggctcgc ggccgcccga
     1621 gcgcgcggcc acgcgagcct cgaaggcctc cctgccgacg aagacgggcg tcgacgcgag
     1681 catcaagtcg tcggccgaga cgcggtccgg tagcgcgaag agcgcgacca ggacggcccc
     1741 cagtatggct ttgcgccccg aggcgcttgt aggagtgagc atggcgttac ctttcacggt
     1801 ctccggcggg tagccacgcg tggctacccg ggtaccagtg gacgatgagg acattccagg
     1861 tggccgacag ggcgccggag cc
//
LOCUS       DAOOLN010000338         1878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_78277_length_1878_cov_2.856829, whole genome shotgun sequence.
ACCESSION   DAOOLN010000338 DAOOLN010000000
VERSION     DAOOLN010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78277_length_1878_cov_2.856829"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PLW80_05080"
     CDS             complement(<1..673)
                     /locus_tag="PLW80_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65911.1"
                     /translation="MRTNRSAWPGARKPGPLRAAVSIAAYDARRSARDMIVLYIIASP
                     LLLALIARFVLPLLAPPPMTVYLAPDVPVQVAVLLGEAGHGIAASPDAADLLLAADPG
                     TPAGLLATVAAGRDDAADLLRAALAGEFRHTRPALADTVAAFLMLSAAFLAALGSGLS
                     IVEERSAGVDRALAVSPVGVGTYFAGKLLFIATVSIAVSLGASLLLYGLDAPFGKLTL
                     AALAAA"
     gene            complement(670..1536)
                     /locus_tag="PLW80_05085"
     CDS             complement(670..1536)
                     /locus_tag="PLW80_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65912.1"
                     /translation="MKAFITLLRQELVVMARNGVSLVLAIVLAASAGSVHAVRAVDDA
                     NQRAVARVGQQVGQQVGSAAGIAGSESFEAGGWTVTVVERGPESNAAEPDQSLADSLV
                     LALLVFEVLVLGFLFVAVGLFQELGEGSLRAYRLSPGGQLRWMAARYALWVGASLAYA
                     TAFLASSFVWPGWQSLAGLLAVTALGSLVMTALGLTIAVFSGGMSGWFIPAVGILMVN
                     MAPMGLADGYPVAYWLTPGGAALAGYRSLFGMGRPGMTGASILWLGTAAVVALPLSWW
                     AVRTRVFKEGRR"
     gene            complement(1533..1838)
                     /locus_tag="PLW80_05090"
     CDS             complement(1533..1838)
                     /locus_tag="PLW80_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65913.1"
                     /translation="MTTHNMYAADELCDRVAFMDKGRILAEGRPRELKLRYGERVAAV
                     EYRDGNELRSERIALDGEAGRARLAELASSGRIETMHSMEASLEAIFIRLTGRKLSA"
BASE COUNT          321 a          683 c          610 g          264 t
ORIGIN      
        1 gcgccgccgc caaggcggcc agcgtcagct tgccgaaggg cgcatccagg ccgtacaaca
       61 ggagcgaggc gccgagtgac acggcgatcg ataccgtagc tatgaatagc agcttgccgg
      121 cgaaataggt acccactccg actggagaca cggctaaggc gcggtcgacg ccggcgctac
      181 gctcctcgac gatcgacagc cccgagccga gcgccgccag gaacgccgcc gacagcatca
      241 ggaaggccgc gaccgtgtcg gctagggccg gtcgcgtatg cctgaattcg ccagcgagtg
      301 ccgcgcggag caggtccgcg gcgtcatctc gtcccgccgc gaccgtggcc aggagcccgg
      361 ccggcgtgcc aggatcggcc gccaggagca ggtccgcggc gtcgggcgac gcggctatgc
      421 cgtggccggc ttcgccgagg aggactgcca cctgaaccgg cacgtccggc gccagataca
      481 cggtcatcgg agggggcgcg agcagcggca ggacgaacct ggctatgagc gccaggagta
      541 gcgggctggc tatgatgtac agcactatca tatctcgggc gctgcgcctg gcgtcgtacg
      601 ccgctatgga gaccgcggcc cgtagcgggc cgggcttgcg cgcgccgggc catgcggatc
      661 gattcgtcct catcgtcggc cctccttgaa cacgcgcgta cgcaccgccc accaggacag
      721 gggcagcgcg acgactgcgg cggtgccgag ccataggatg gaggcgcccg tcatgcccgg
      781 ccgccccatg ccgaacaggc ttctataccc cgcgagagcc gctccgcccg gcgttagcca
      841 gtacgctacg gggtagccgt cggccaggcc catcggcgcc atgttcacca tcaggatccc
      901 gaccgccgga atgaaccatc ccgacatacc gccggagaat acggcgatcg tcaggcccag
      961 cgccgtcatc acgagcgatc cgagcgccgt caccgccaac aacccggcca ggctctgcca
     1021 gccgggccag acgaacgaac tcgccaggaa ggccgtagcg taggcgaggc tggctccgac
     1081 ccacagcgcg taccgcgccg ccatccatcg tagctgaccg ccagggctca gccgatacgc
     1141 gcgtaggctg ccttcgccca gctcctggaa caggcccacg gccacgaaca ggaagccgag
     1201 taccagtacc tcgaacacca agagcgccaa cacgaggctg tccgccagag attggtctgg
     1261 ctccgccgcg ttcgactcgg ggccgcgctc gacgacggtc accgtccatc cgccggcctc
     1321 gaacgactcg gaaccggcta ttcccgccgc cgagccgacc tgctggccga cctgctggcc
     1381 gacccgagcg acggcgcgtt ggttcgcgtc gtcgaccgct cgtacagcgt ggacgctccc
     1441 ggccgaggcc gccagcacga tggccagcac gagcgacacc ccgttccgcg ccatgacgac
     1501 gagctcctgc ctgagtagcg tgatgaaggc cttcatgcgg acagcttcct gcccgtcagc
     1561 cggatgaaga tggcttccag gctcgcttcc atcgagtgca tcgtctcgat ccggcctgag
     1621 gacgccagct cggccagcct ggctcggccg gcctcgccgt cgagcgctat ccgctcggat
     1681 cgtaattcgt tcccgtcccg gtactccacc gcggccactc gctcgccgta gcgcagcttc
     1741 agctcgcgcg ggcgaccttc ggcgaggatc ctgcccttgt ccatgaacgc gaccctgtcg
     1801 catagctcgt cggcggcgta catgttgtgc gtcgtcaaga gtatcgtggc gccacggtcg
     1861 cgctactccc gtatcatg
//
LOCUS       DAOOLN010000339         1874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_78534_length_1874_cov_3.294667, whole genome shotgun sequence.
ACCESSION   DAOOLN010000339 DAOOLN010000000
VERSION     DAOOLN010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78534_length_1874_cov_3.294667"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PLW80_05095"
     CDS             <1..627
                     /locus_tag="PLW80_05095"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HPB65914.1"
                     /translation="KNAEGDTGLEGAIILTQPPELKSVNIATSWAENRRAFYYNRKLN
                     CMKASGGFTIGRKRYEFDPAKDFGELDWGRGRWTYKNRWYWGSAAGLAGGVPFGWNIG
                     YGFSDRSPASENAVFYAGKVHKLDEVTFHIDTADYLAPWRFSSSDGRFEMDFSPLVDR
                     SSTMAIGPIKSVQHQVFGEFTGTAILDDGQAIRADRLLGFAEDVLNWW"
     gene            complement(634..1227)
                     /locus_tag="PLW80_05100"
     CDS             complement(634..1227)
                     /locus_tag="PLW80_05100"
                     /inference="COORDINATES: protein motif:HMM:NF017466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid recognition domain-containing
                     protein"
                     /protein_id="HPB65915.1"
                     /translation="MVASNTASIDIFERIVSLVHEATGHGVNIMGDGGRILASSDPTR
                     VGTVHDGGRRVMSGEVEEVAIDADTAATLRNVKAGYMGAVRMDSKLIACIGIGGEPHE
                     VKPLQKMAALSLKQELERERLAGRERGLLQDVRRDIGDIAERMQVLSLNGAVLAARLG
                     EKGRGFKIVVGEMRELAAQIGEKLVELERREKNVVSE"
     gene            complement(1303..1569)
                     /locus_tag="PLW80_05105"
     CDS             complement(1303..1569)
                     /locus_tag="PLW80_05105"
                     /inference="COORDINATES: protein motif:HMM:NF017013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HPB65916.1"
                     /translation="MAALGRSPKDGERLTFLSAGTDGNDGPTEAAGAVVSLGLYRSAV
                     AAGLDPEAALADNDSNGFFRKAGGLVVTGPTNTNVCDVQVLIVT"
     gene            complement(1527..>1874)
                     /locus_tag="PLW80_05110"
     CDS             complement(1527..>1874)
                     /locus_tag="PLW80_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65917.1"
                     /translation="TPKADDPAFARTRTLLVGTNRLALAAAEAEARRLGYNSLVVSSR
                     VSGEAREIAMAFLGIGKDIAASDFPVAKPACVIAGGETTVTCAEAARAAVTRRWRSPS
                     WPRSAARPRTESG"
BASE COUNT          309 a          644 c          597 g          324 t
ORIGIN      
        1 aagaacgccg aaggcgatac gggcctcgag ggcgccatca tcctgaccca gccgcccgag
       61 ctcaaaagcg tcaacatcgc cacctcctgg gccgagaacc gccgcgcgtt ctactacaac
      121 cgcaagctca actgcatgaa ggcctcaggc ggtttcacga taggcaggaa gcgctacgag
      181 ttcgaccccg ccaaggactt cggggagctg gactggggac ggggccgctg gacctacaag
      241 aaccgctggt actggggctc ggccgccggg ctcgcgggcg gcgtaccctt cggctggaac
      301 atcggctacg gcttctccga ccgcagcccg gccagcgaga acgccgtctt ctatgccggc
      361 aaggtccata agctcgacga ggtgaccttc catatcgata cggccgacta cctcgccccc
      421 tggcgcttct cgagttccga cggccgtttc gagatggact tctcgccact cgtcgaccgt
      481 agctcgacca tggccatcgg cccgatcaag tcggtccagc accaggtctt cggcgagttc
      541 accggcacgg ccatactcga cgacggccag gcgatccggg cggaccgcct cctcggcttc
      601 gcggaggacg tactcaactg gtggtaggcg tcgtcactcg gagacgacgt tcttctcccg
      661 gcgctcgagc tcgacgagct tctcgcctat ctgggcggcg agctcgcgca tctccccgac
      721 gacaatcttg aagccgcggc ccttctcgcc gagcctcgcg gcgagcaccg cgccgttcag
      781 ggacagcacc tgcatgcgct cggctatgtc gccgatgtct cgccgcacgt cctggagcag
      841 gccgcgttcc ctaccggcga gccgttcccg ctcgagttcc tgcttgagcg atagcgcggc
      901 cattttctgg agcggcttga cctcgtgagg ctcgccgcct atgccgatgc acgctatcag
      961 cttgctgtcc atgcgaaccg cgcccatgta gccggccttg acgttacgca gggtagctgc
     1021 ggtgtcggcg tctatggcga cctcctcgac ctcgccgctc atcacccgcc ggccgccgtc
     1081 atggacggta ccgacgcgcg tcggatcgga ggaggccagg atgcggccac catcgcccat
     1141 gatattgacg ccatgaccgg tggcctcatg aacgagcgag acgattcgtt cgaatatatc
     1201 gatagaagcc gtattggacg cgaccattca ggttccttaa gcgctaggat acgcgcaatg
     1261 taccatgtat acacgtagtg ctcaaccagg attcttagcg gactaggtga cgatgaggac
     1321 ctgtacgtcg cagacgttcg tgttcgtcgg accggttacg acgaggccac cggctttcct
     1381 gaagaagccg ttggagtcgt tgtcggcgag cgccgcctcc ggatccaggc cggcggcgac
     1441 ggccgagcgg tacaggccca ggctgaccac ggcgcccgcg gcctcggtcg gcccgtcgtt
     1501 gccatcggtg ccggcggaca ggaaggtcag ccgctctccg tccttgggcg agcggccgag
     1561 cgcggccatg aaggcgagcg ccatctcctg gttacggccg cccttgccgc ctccgcgcag
     1621 gttacggtcg tctcgccgcc tgcgatgacg caggccggct tggccaccgg gaaatcggac
     1681 gccgcgatgt ccttgccgat gcccaggaac gccatggcga tctctcgggc ctcgccagaa
     1741 acgcgcgacg acactacgag gctgttgtag ccgagccgcc gggcctcggc ctcggccgcc
     1801 gcgagcgcca gccggttcgt gccgacgagc agggttctcg tgcgggcgaa ggccgggtcg
     1861 tccgccttgg gcgt
//
LOCUS       DAOOLN010000340         1873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_78599_length_1873_cov_2.752475, whole genome shotgun sequence.
ACCESSION   DAOOLN010000340 DAOOLN010000000
VERSION     DAOOLN010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78599_length_1873_cov_2.752475"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1788)
                     /gene="lon"
                     /locus_tag="PLW80_05115"
     CDS             complement(<1..1788)
                     /gene="lon"
                     /locus_tag="PLW80_05115"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPB65918.1"
                     /translation="MLLPNKIPIIPLNGKPIFPGIFTPLMLGSQDDIQTIDDAMQTDG
                     MVGLVLVRDDTDKPKASDLHEVGTVAKIVKRINLPDGSVNIFISTLKRFKIKKALSKE
                     QPIMAAVEYLEDQGEGPDEIKALTRALLSEMKQISENNPLFSEEMRLNMINIDHPGKI
                     ADFIASILNIDKVEQQKILELSDIKERMERVLMSIKREQELLKIQKRIQSEINEKVEK
                     SQRDYFLREELKAIKQELGMPADAKGTDFQRFKERLDAFGFSGEVKELVDQELEKFSL
                     MDPNSSEFIVTRNWLDLVSNLPWKAETGKDFDIHAARDILEGDHYGLKDVKDRIIEYL
                     AVRKLKRDAKGSILCLVGPPGVGKTSVGRSIARALGKEFFRFSLGGMRDEAEIKGHRR
                     TYVGALPGKIIQGLKITKSRDPVFMIDEIDKMGSSYQGDPSSALLEVLDPEQNVAFRD
                     HYLDLPFDVSNVFFIVTANTLDTIPGPLRDRMEIIQLSGYVDSEKLEIAKRYLIPKSL
                     EKNGLKKARVKYTRDALTAIAEGYAREAGVRNFEKYLDRIHRKIALAVVTAPEGEDTK
                     RFEVDKPSLEKYLGKPRFDADEVKRATLPG"
BASE COUNT          321 a          536 c          563 g          453 t
ORIGIN      
        1 gcccggcagc gtcgcgcgct tgacctcgtc ggcgtcgaag cgcggcttgc ccaggtactt
       61 ctcgagtgac ggcttgtcca cctcgaagcg cttcgtatcc tcgccttcgg gcgccgttac
      121 gacggccagc gctatcttgc gatggatgcg atccaggtac ttctcgaagt tccggacgcc
      181 ggcctctctc gcgtagccct cggcgatggc ggttaacgcg tcgcgggtgt acttgacgcg
      241 ggccttcttg aggccgttct tctcgaggct cttcgggatc aggtagcgct tggcgatctc
      301 gagcttctcc gagtcgacgt agccggatag ctggatgatc tccatgcggt cgcgtagcgg
      361 cccggggatg gtgtcgaggg tattggccgt gacgatgaag aacacgttgg agacgtcgaa
      421 gggtaggtcg aggtaatgat cccggaacgc cacgttctgc tccgggtcga gcacctcgag
      481 cagggcgctc gacggatcgc cctggtagct cgagcccatc ttgtcgatct catcgatcat
      541 gaagaccggg tcgcggctct tggttatctt gagcccttgg atgatcttgc cgggtagcgc
      601 cccgacatag gtgcgccgat ggcccttgat ttccgcctcg tcgcgcatgc cgccgagcga
      661 gaagcggaag aactccttgc cgagagcccg ggcgattgat ctaccgacgc tggtcttgcc
      721 gacgccgggc gggcctacca ggcacaggat agagcccttg gcgtcgcgtt tgagcttacg
      781 gactgccaga tactcgatga tgcgatcctt gacgtccttg aggccgtagt ggtcgccctc
      841 gaggatgtcg cgggcggcat gtatgtcgaa gtccttgccg gtctccgctt tccaaggcag
      901 gttcgagacc agatcgagcc aattcctcgt cacgatgaac tccgacgaat tcgggtccat
      961 caggctgaat ttctcgagct cctggtcgac gagctccttg acctcgcccg agaagccgaa
     1021 cgcgtccagc cgctccttga agcgttggaa gtccgtgccc ttcgcgtcgg cgggcatacc
     1081 gagctcctgc ttgatggcct tgagctcctc ccgcagaaaa tagtcgcgct ggctcttctc
     1141 gaccttctcg ttgatctcgc tctggatgcg tttctggatc ttgaggagct cctgctcgcg
     1201 cttgatcgac atgaggacgc gttccatacg ctccttgata tcgctgagct cgagaatctt
     1261 ctgctgctcg accttgtcta tattcaggat gctggcgatg aagtcggcta tcttgcctgg
     1321 atggtcgatg ttgatcatgt tgaggcgcat ctcctcggag aacagtggat tgttctccga
     1381 gatctgcttc atctccgaca gcagcgccct ggtcagcgcc ttgatctcgt ccgggccttc
     1441 cccctggtcc tcgaggtact cgaccgccgc catgatgggc tgttccttgg acagggcctt
     1501 cttgatcttg aagcgcttga gggttgagat gaatatattg accgagccgt cgggaaggtt
     1561 gatccgcttg acgatcttgg ctaccgtacc cacctcgtgg aggtcgctgg ccttgggctt
     1621 gtcggtatcg tcccggacga gcacgagccc gaccatgccg tcggtctgca tagcatcgtc
     1681 gatggtctgg atgtcgtcct gactcccgag catcaagggc gtaaagatgc cggggaatat
     1741 aggctttcca ttcaacggaa tgattggaat tttattcggt agtagcatgt cgatcggtat
     1801 tatctcgtat gccgtcttct gcttgaaaaa aaaaaaaaag tttcgttata aaatatagag
     1861 gttttggacc gac
//
LOCUS       DAOOLN010000341         1872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_78654_length_1872_cov_3.157402, whole genome shotgun sequence.
ACCESSION   DAOOLN010000341 DAOOLN010000000
VERSION     DAOOLN010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78654_length_1872_cov_3.157402"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..>1872
                     /locus_tag="PLW80_05120"
     CDS             189..>1872
                     /locus_tag="PLW80_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65919.1"
                     /translation="MHRTLALVFATAVFVSAASADLFSPDRPLSTVETEYFTFIYAAE
                     SEPAVRYLASFADDAYREIAALLGTSPRARLPVVMTPDSETLNGYYTAYPYPRIVLYQ
                     APTEAGSSLGSFDDDLRKLFQHELTHAVSLSLRGGAEGALVAVFGSPLGLSTYLAPRA
                     FVEGVTVSFESMNGFGRATDPLAGAIIRQDIVEGRGRSFAQACGADDRYPSSALHYLY
                     GGYFSRYLQGRWGIGKYAELWTLFGARALLRPLDDSWLGEGRFSRVYGISLSEAWTDF
                     MRSMAPTAPVYMAAEPRTDASSVSALCSSGDALYYADEAAETVYRLDPVSGDSRPLFR
                     AGAEVSRLDASPDGSALLVSTTRLAGGFPKLAVMEYTVGGRGGPRLLPLSRVRDAAYL
                     PGGGLVGVSVDGYETDLVVARGEARSTLLEGNERTNFSSPVASADGAYVYAIARLDGV
                     PSIIRLALDGTGTRAVSVERLIVPDGLSWLRYLSMGTDGVLRSGWDDGSFYRLVELDD
                     GLIRYQSVPISGGVHCPVLSAGLVRYIGRFSDGQRLCDYPADRTPLGFAEVPA"
BASE COUNT          249 a          678 c          616 g          329 t
ORIGIN      
        1 ggcgacgctc aaggctctcg tcgagcgttt cacggtctcc atggccatgg aaggtagttc
       61 gacgatagaa cgataaggac ggccggcgcg ctgcggcgcc ggcccggagg cgcgacggat
      121 gttgcgcctc cggccgtttt gtccgtatac tccacgggcg gcggcgcctc gtcgcaccgg
      181 gggtatgcat gcatcggacg ctcgccttag tgttcgcgac cgcggtcttc gtctccgcgg
      241 cgtccgccga cctgttctcg cccgacaggc ctttgtccac agtcgagacc gagtacttca
      301 cgttcatcta cgcggccgag tcggagccgg cggtacggta ccttgcttcc ttcgccgacg
      361 acgcgtaccg cgagatagcc gcgctgctcg gcacgagccc ccgcgcgagg ctgccggtcg
      421 tcatgacgcc cgattccgag acgctgaacg gctactacac cgcctatccc tatccgcgca
      481 tcgtgctgta ccaggccccc accgaggcag gctcgagcct cggatcgttc gacgacgatc
      541 tgagaaagct gttccagcac gagctcacgc acgcggtgtc gctaagcctc cgggggggcg
      601 cggagggcgc gctcgtcgcc gtcttcggct cgccgctcgg cctgtcgacg tatctagcgc
      661 ccagggcttt cgtcgagggc gtcaccgtgt cgttcgagag catgaacggc ttcggccgtg
      721 cgaccgaccc gctcgccggc gccatcatac gccaggacat cgtcgaaggc cggggcagga
      781 gcttcgccca ggcctgcgga gccgacgacc gctacccatc gagcgcgctc cactacctct
      841 atggcggcta cttctcgcgc tacctgcagg ggcgctgggg cattgggaag tacgccgagc
      901 tctggacgct cttcggcgct agggcgctcc tgcgaccgct cgacgacagt tggcttggcg
      961 aggggcgctt ctcgcgggtc tacggcatat cgctatcgga ggcctggacc gacttcatgc
     1021 gctccatggc gccgacggcg cccgtctaca tggccgccga gcctcggacg gacgcgtcgt
     1081 ccgtctcggc gctctgctcg tcgggcgacg cgctctacta tgccgacgag gcggccgaga
     1141 ccgtctaccg gctcgacccg gtctccggcg atagccgccc gctgttcagg gccggcgccg
     1201 aggtgtcgcg gctcgacgcc tcgccggacg gctcggccct cctcgtctcc acgacccgcc
     1261 tcgcaggcgg tttccccaag ctcgctgtca tggagtacac ggtcggcggc cgaggcggcc
     1321 ctcggctcct gcccctgagc cgcgtcaggg acgcggccta cctacccggc ggcgggctgg
     1381 tcggggtgtc cgtcgacggc tacgagaccg acctggtcgt cgcgcgcggc gaggcccgct
     1441 ctacgctact cgagggcaac gagcgtacga atttctcgtc gcccgtggcc tccgccgacg
     1501 gagcctacgt ctacgcgatc gccaggctcg acggcgtccc ctcgatcatc aggctagcgt
     1561 tggacgggac cgggacccgc gcggtctcgg tagagcgcct catcgtcccc gacggcctct
     1621 cctggctgcg ctatctatcg atgggcacgg acggcgtcct acgttccggc tgggatgacg
     1681 gttcgttcta tcgcctcgtc gagctcgacg acgggctgat acggtaccaa agcgtcccga
     1741 tctcgggcgg cgtccactgc ccggtactgt ccgccggcct ggtgcgctat atcggccgct
     1801 tctccgacgg ccagcgcctc tgcgattacc cggcagaccg cacgcctctc ggcttcgccg
     1861 aggttccggc gc
//
LOCUS       DAOOLN010000342         1871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_78740_length_1871_cov_2.905837, whole genome shotgun sequence.
ACCESSION   DAOOLN010000342 DAOOLN010000000
VERSION     DAOOLN010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78740_length_1871_cov_2.905837"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PLW80_05125"
     CDS             complement(<1..512)
                     /locus_tag="PLW80_05125"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HPB65920.1"
                     /translation="MLLLVGPYALLAILAIAARQLGWKAAGAAKALPLSFLIAFLAAA
                     AATGRAEAGLVYWLIMGGLACGLAGDILLLDKGRFFKQGVVAFLGGHVLYIAAFALSG
                     LRPTWRMLMPIAGYFGLYAIVLFNILLAKHAKYRPLAIAYLGGVLAMTVSALFADAAN
                     AARGAPSYFF"
     gene            595..1407
                     /locus_tag="PLW80_05130"
     CDS             595..1407
                     /locus_tag="PLW80_05130"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPB65921.1"
                     /translation="MIPMAICYDFDGTLAPGNMQEYDFIPAVGVSPKEFWAAAQELAE
                     RHEADTILAYMLLMLRKAAEAGVKVTRDSFRDFGSTIRLFPGVESWFGRIDAYAASRG
                     VHVDHYVISSGLREMIAGTSVAGAFTKIYASGFLYDDEGEAVWPALAVNYTNKTQFLF
                     RINKGSPEVYDNSRVNAYVPKDERSLPFSNMVYLGDGETDVPCFRLVDKEGGWAVAVY
                     PPDDDAAAEKTRRLVDLGRAKVAAPADYSEGSSLELRVQAIVDHIEAGCRIR"
     gene            complement(1562..>1871)
                     /gene="eno"
                     /locus_tag="PLW80_05135"
     CDS             complement(1562..>1871)
                     /gene="eno"
                     /locus_tag="PLW80_05135"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260860.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPB65922.1"
                     /translation="AMGACNSILIKLNQIGTVTETIEAINMAKEAGYTTVVSHRSGET
                     EDTFLADLVVACETGQIKTGSMSRTDRIAKYNQLMRIEDAMEGIAEYPGMKAFYNIKR
                     "
BASE COUNT          338 a          603 c          627 g          303 t
ORIGIN      
        1 tcgaagaaat agctgggcgc gcctcgggcg gcgttagcgg cgtcggcgaa gagggccgac
       61 accgtcatcg cgagcacgcc gccgaggtag gcgatggcca gcggccgata cttggcgtgc
      121 ttggcgagca ggatgttgaa cagcacaatc gcgtagaggc cgaagtaccc ggcgataggc
      181 atgagcatgc gccaggtagg cctcagcccg gagagcgcga aggcggcgat atacaagacg
      241 tggccgccca ggaaggcgac gacgccctgc ttgaagaagc gccccttgtc cagcagcagg
      301 atatcccccg ccaggccgca tgccagcccg cccatgatca gccagtacac caacccggcc
      361 tcggcccgcc cggtcgcggc cgccgcggcc agaaacgcga tcaggaacga taggggcagg
      421 gccttggccg cgcccgccgc cttccagccg agctgacggg cggcgatggc caggatcgcc
      481 aggagcgcgt acggaccgac gagcagtagc atctatacct ccgggttcat ggacccatcg
      541 attctaccat ggattcggct cctggtaata ccagcgatgg tgtatactcg agccatgata
      601 ccgatggcta tctgctacga tttcgacggc accctggctc ccgggaacat gcaggagtac
      661 gacttcatac cagccgtcgg cgtatcgccg aaggaattct gggccgccgc ccaggagctc
      721 gccgagcggc acgaggccga cacgatcctc gcctacatgc tcttgatgct gcgcaaggcg
      781 gccgaggcgg gcgtcaaggt gacgagggac tcgttcaggg atttcgggtc gacgatacgg
      841 ctcttccccg gcgtcgagtc gtggttcggc cgcattgacg cgtacgcggc cagccgcggc
      901 gtccacgtcg accactacgt catatcgtcg ggcctgcgcg agatgatagc gggcacgagc
      961 gtcgccggcg cgttcaccaa gatctacgcg tccggtttcc tgtacgacga cgagggcgag
     1021 gcggtctggc cggcgctcgc ggtcaattat acgaacaaga cccagttcct gttcaggatc
     1081 aacaagggta gccccgaggt ctatgataac agccgcgtca acgcgtacgt gcccaaggat
     1141 gagcgctcgc taccgttctc gaacatggtc tacctgggcg acggggagac cgacgtgccg
     1201 tgcttccggc tcgtcgataa ggagggcggc tgggccgtag cggtctaccc cccggacgac
     1261 gacgcggccg ccgagaagac ccggcgcctc gtcgacctgg gccgcgctaa ggtcgccgcc
     1321 ccggctgact actcggaagg ctcgagcctc gagctgcgcg tccaggcgat cgtcgatcat
     1381 atcgaggcgg gttgccggat acgatgagcg ccagaggtct cgacggccga acctggcggg
     1441 cctagcctcg ccgacagtgc catacagccc gattccagcg cctcgcgcgg gaacgcgcgc
     1501 caaaaaaacc gccccccgag gggggcggcc acggatcgcc cacgcgcggg cgggatgcta
     1561 cttacctctt gatgttgtag aacgccttca tccccggata ctccgcgatg ccttccatcg
     1621 cgtcctcgat gcgcatcagc tggttgtact tggcgatgcg gtcggtgcgc gacatcgagc
     1681 cggtcttgat ctggccggtc tcgcaggcga cgacgaggtc cgcgaggaag gtgtcctcgg
     1741 tctcgccgga gcggtgggac acgacggtcg tgtagcccgc ctccttggcc atgttgatgg
     1801 cctcgatggt ctcggtgacg gtgccgatct ggttgagctt gatcaggatg gagttgcagg
     1861 cgcccatcgc g
//
LOCUS       DAOOLN010000343         1866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_79069_length_1866_cov_2.927664, whole genome shotgun sequence.
ACCESSION   DAOOLN010000343 DAOOLN010000000
VERSION     DAOOLN010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79069_length_1866_cov_2.927664"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PLW80_05140"
     CDS             complement(<1..702)
                     /locus_tag="PLW80_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65923.1"
                     /translation="MNRDTLSSRFSAGRVMLLARNRAYDDAPGLGIGLAIVAGLNLLS
                     ILIADRAFMNSEGDPSYAGLIIISGLILSSQAFKGMHGRTGADWILLPATPLEKYAAA
                     FVSYLVVFPLVAAAAATGLSFVLALVERAAGGPGTGIWHPISLAALRSYGEYAVVAVA
                     FLAGSAAFRKRAFLKTAGLSVAFVLLCSLVVYLGLSLVHEATGSRGFSVSYDSGSLTV
                     LGGDGPGALSVERTLR"
     gene            complement(699..1580)
                     /locus_tag="PLW80_05145"
     CDS             complement(699..1580)
                     /locus_tag="PLW80_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB65924.1"
                     /translation="MESMTFGYGKRPLFEDLDLRLEPGAIHGLLGLNGAGKTSLLKIA
                     AGALRPERGSVEVYGRAPGKREAAALADVCFVPEDPCFPALKPAAWLGRYAAFRPAFD
                     HGRFDELFAELGLDADKPISKFSYGQRKKFALAAAVASGSRLLLLDEPTNGLDIPSKA
                     QLRRVLAAASGPDRVVVVSTHQIRDLELLLDPIIVIDRGRVAFTVTMAELSDRLGAAR
                     LPSIEGRPVVYAERDAVGWTALLATAGPGEAAPPDLELVFNAAIAAPDRLAAALRGDA
                     LPEYRPEAAALAAGGRA"
BASE COUNT          354 a          645 c          614 g          253 t
ORIGIN      
        1 gcgcagcgtg cgctcgaccg agagggcgcc cggcccgtca ccgccgagga cggtcagcga
       61 cccggaatcg tatgatacgg agaagccgcg ggaacccgta gcctcgtgga ccagcgatag
      121 acccaggtac acgaccaggg aacagaggag cacgaaagct accgacaggc cggcggtctt
      181 caggaaggcg cgcttgcgga acgccgcgga gccggccagg aaggccacgg ctacgaccgc
      241 gtactctccg taactacgga gcgcggcgag cgaaatcgga tgccagatcc cggttcccgg
      301 ccctccggcc gcccgctcga ccagggccag cacaaacgac agccccgtag cggccgccgc
      361 cgcgacgagc gggaagacga ccaggtacga tacgaatgcg gccgcgtact tctcgagcgg
      421 cgtcgccggc agcaggatcc agtcggcgcc ggtccgccca tgcatgccct tgaaggcctg
      481 cgacgacagg atgagcccgg agatgatgat cagccccgcg tagctcggat cgccctcgct
      541 gttcatgaag gcgcggtcgg cgataagaat agaaagaaga ttcagcccgg ccacgatggc
      601 cagcccgata cccaggcccg gcgcgtcgtc ataggccctg ttccgcgcca ggagcatgac
      661 ccggcccgcg ctgaagcggc tcgatagggt atcgcggttc atgccctgcc tcccgcggcg
      721 agcgccgctg cctcgggccg atactcgggt agcgcgtccc cgcgcagcgc ggccgcgagc
      781 ctgtcgggcg ccgcgatggc cgcgttgaac acgagctcca ggtccggcgg cgccgcctcc
      841 cccgggcccg cggtcgccag gagagcggtc cagccgaccg cgtcgcgctc ggcgtagacg
      901 acgggccggc cctcgatcga cggcagtctg gcggcgccga gccgatcgga cagctcggcc
      961 atcgtcaccg tgaaggccac ccggccccga tctatcacga tgatgggatc gagcaggagc
     1021 tcgaggtcgc ggatctggtg ggtcgacacg acgacgacgc ggtcgggccc agaagccgcc
     1081 gccagcacgc gcctcagctg cgccttggac gggatatcga gcccgttggt cggctcgtcc
     1141 aggaggagaa gcctggagcc actcgccacc gccgcggcca aggcgaattt cttacgctgg
     1201 ccatacgaga atttggatat cggcttatcg gcgtccagtc cgagctccgc gaacagctca
     1261 tcgaagcgcc cgtgatcgaa ggccggcctg aacgcggcgt agcgcccaag ccatgcggcc
     1321 ggcttcaggg ccgggaagca cggatcctcc ggcacgaaac agacgtcggc cagcgcggcc
     1381 gcctcccgct ttccgggagc ccgtccgtag acctcgacgc tgccccgctc cggcctgagg
     1441 gcgccggccg cgatcttcag aagactcgtc ttgccggccc cgttgagtcc caggagaccg
     1501 tggatggctc ccggttccaa tcgtaggtcg aggtcctcga agagcggcct cttcccatac
     1561 ccgaaggtca tggattccat acgtaccatt acctactcct tgagtgtact aggttattaa
     1621 cacagtaata caaacaccgc cacccgtcaa gtccttggaa cgccgctcgt agcgccatgg
     1681 acgcgcgccg atattgcatg cgggctcagg acgtgcgata gttcggcgta tgaacgaaga
     1741 acgaaacggc gccggccggc gccgtgaccc gtcgcgcgat cgacggccag aagaacgcaa
     1801 acagccggat caggctcgcc ggacaaaata cgtagcgccg ccgcgaacgg tttacgaacc
     1861 gtcaag
//
LOCUS       DAOOLN010000344         1863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_79265_length_1863_cov_2.491704, whole genome shotgun sequence.
ACCESSION   DAOOLN010000344 DAOOLN010000000
VERSION     DAOOLN010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79265_length_1863_cov_2.491704"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PLW80_05150"
     CDS             complement(<1..426)
                     /locus_tag="PLW80_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65925.1"
                     /translation="MSGREDFAVNLASRHARGDRWRLMFMLSTLLAIVFLGALILTIV
                     DDAFGYVVMVDEVDQRSLVPDGERLEGLDAEALRAVIDERLSPRRYRAIERERPFDER
                     TAADLYDIVLYEIVKPRVAETYTLFESLFARRSIVEETTL"
     gene            complement(423..1364)
                     /gene="pstC"
                     /locus_tag="PLW80_05155"
     CDS             complement(423..1364)
                     /gene="pstC"
                     /locus_tag="PLW80_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPB65926.1"
                     /translation="MKPVQTARYPEEARGALSFGKRFRPHEAVILALLFFCAAVSILT
                     TLGIIVVLGAESSALFKEVSLWEFVSGTSWQPTIGEFGILPLVNATLMTSLAAMLVAL
                     PLGLGVAIYLAEYASERVRETLKPILEVLAGVPTVVYGYFALTVVTPALRGLFGQESI
                     EVYNTLSAGLVMGILILPLISSMCEDALSAVPRSLREAAYAMGATKLETSVTIIVPAA
                     FSGIAAAFILGLSRAVGETMIVALAAGAGPNLTLNPLKAAETMTGHIVRISGGDISYD
                     SMDYRSLFAIALVLFLATLALNMVSRSIVKKYREVYE"
     assembly_gap    1524..1533
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1534..>1863)
                     /locus_tag="PLW80_05160"
     CDS             complement(<1534..>1863)
                     /locus_tag="PLW80_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HPB65927.1"
                     /translation="GTDSGTFDYFVEHLYRKDRKPILDAKNLQLSEDDNVLVRGVSGD
                     AYAIGLFGFAYYQENKDKLTVLSIDTVEANAANVDNGTYPLARPIFIYSDAKIMNDKP
                     QVAAFIA"
BASE COUNT          317 a          585 c          620 g          331 t
ORIGIN      
        1 tagagtcgtc tcctcgacga tgctcctcct cgcgaacagg gattcgaaca gggtgtacgt
       61 ctcggcgacc cgcggcttga cgatctcgta cagtacgatg tcgtacagat cggctgcggt
      121 ccgctcgtcg aacggccgct ccctctcgat cgcgcggtag cgacgggggc tcagccgctc
      181 gtcgatgacg gcgcgcaagg cctcggcgtc caggccttcg agcctctcgc cgtccggtac
      241 gaggctccgc tggtcgacct cgtcgaccat gaccacgtag ccgaacgcgt cgtcgacgat
      301 ggtcagtatc aacgccccga ggaacacaat ggcgagcagg gtcgacagca tgaacatgag
      361 ccgccaacgg tcgcccctcg cgtgcctcga ggcgaggttg acggcgaagt cctcgcggcc
      421 gctcattcgt acacctcgcg gtatttcttg actatgctcc tggagaccat gttgagcgcg
      481 agcgtcgcga ggaacagcac gagcgctatc gcgaacaggc tacggtagtc catcgagtcg
      541 taggatatgt cgccgccgga gatcctgacg atgtggccgg tcatcgtctc ggcggccttg
      601 agcgggttga gcgtgaggtt cggccccgcc ccggccgcga gcgcgacgat catcgtctcg
      661 cctacggcgc gcgacaggcc gaggatgaag gccgcggcga tcccggagaa ggccgccggc
      721 acgatgatcg tcacgctggt ctccagcttg gtcgcgccca tcgcgtaggc cgcctcgcgg
      781 aggctccgcg ggacggcgga gagcgcgtcc tcgcacatcg agctgatcag cggcagtatc
      841 aggatgccca tcacgagccc ggccgatagg gtgttgtaga cctcgatcga ctcctggccg
      901 aacaggccgc gcaacgcggg cgtgacgacc gtcagggcga aatacccgta tacgacggta
      961 ggtacgcctg cgagcacctc gagtatcggc ttcagcgtct cgcggacgcg ctcggacgcg
     1021 tactcggcca ggtagatggc cacgccaagg cccaggggca gggcgacgag catcgccgcg
     1081 aggctggtca tcagcgtcgc gttgacgagc ggcaggatgc cgaactcccc gatcgtgggt
     1141 tgccaggacg taccgctgac gaattcccag aggctcactt ccttgaacaa ggccgacgac
     1201 tcggcgccca gcaccacgat gatgccgagc gtcgtgagga tggataccgc ggcgcagaag
     1261 aacaggagcg ccaggatgac ggcctcgtgg ggccggaagc gcttcccgaa ggaaagcgcg
     1321 ccgcgcgcct cctccgggta cctcgccgtc tggactggtt tcatcgttct cgctttcagc
     1381 tggagccgcg cgccctcgct atgaacgggg cttcgtttta gtagcggccc ttgacggcgt
     1441 ccagccatgc ctgcttggcc ttcttctgct ccgccgcggg ggcggggaag taaccgactt
     1501 ccttgagcgc ctcgttcacg tagnnnnnnn nnnaggcgat gaaggcggcc acctgcggct
     1561 tgtcgttcat gatcttcgcg tcgctgtaga tgaagatcgg gcgggcgagg gggtaggtgc
     1621 cgttgtcgac gttagccgcg ttggcctcga ccgtgtcgat cgacagcacg gttagcttgt
     1681 ccttgttctc ctggtagtac gcgaaaccga agaggcctat cgcgtaggcg tcgcctgaga
     1741 cgccgcggac cagcacgttg tcatcctcgg aaagctggag attcttcgcg tcgaggatcg
     1801 gcttgcggtc cttcctgtac aggtgctcga cgaagtagtc gaaggtgccg gaatcggtac
     1861 cgg
//
LOCUS       DAOOLN010000345         1862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_79304_length_1862_cov_3.147759, whole genome shotgun sequence.
ACCESSION   DAOOLN010000345 DAOOLN010000000
VERSION     DAOOLN010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79304_length_1862_cov_3.147759"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..881)
                     /locus_tag="PLW80_05165"
     CDS             complement(210..881)
                     /locus_tag="PLW80_05165"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPB65928.1"
                     /translation="MDIPSVLPPSLLAERLESLAYLYKRADSAVDAFVAASGVSCPFG
                     CGSCCEGFVPDILPVEAAYLAAFLAAADRPKAYEAAAGRLGVRSYPDGRQGCPLYRTD
                     TPYHCGVYEARPLICRMFAFSATRDKLSRTAFSVCRLGSSRGGVRGASGEALLDAFGA
                     EPPVMADYGSELAGIEPESAGRRMPLPEALAEAASRVMFLIGIRDDNPLDSGPDVRPP
                     LPNAS"
     gene            complement(1022..1432)
                     /locus_tag="PLW80_05170"
     CDS             complement(1022..1432)
                     /locus_tag="PLW80_05170"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPB65929.1"
                     /translation="MPRRFPFPTTATAALILVAVLLPGSALPEGPGVPGLDKLVHAVL
                     FFGLALAAQLDFGLDGRRRVAVALVAALIFSALTEALQLAVDGRSSELLDMAADMGGF
                     VVGLACRRPLARAARRAAAIVVSRLSGSGGRRGA"
     gene            complement(1436..>1862)
                     /locus_tag="PLW80_05175"
     CDS             complement(1436..>1862)
                     /locus_tag="PLW80_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPB65930.1"
                     /translation="VVGLAKENEELWPHGAREPVVLPKDSPALRLVVAVRDEAHRFAT
                     GMNQRLRGKALGFPVLEAVKGVGPTRARRIMDAFGSLAAVAEAEPSYIAARSGLDGKT
                     AEAVRDAASRAASAMGVAASSSAAGGTAGLDEVVGPGRS"
BASE COUNT          292 a          681 c          676 g          213 t
ORIGIN      
        1 cccgagccgg ccaaggcccg gtcgccggtc aggccgcgtt gcagggccag caggaccacg
       61 gagctcgggc ggccgtcgga tggtcgacgg cggcggtcag gcatcggacc tggcgcctga
      121 cgccgaactt gcgcgagtgg aacgtttcat cgccgtcgac gataccagcg gggaacgacg
      181 atggcaagcg ccgccccggg acggggccac tagctcgcgt tcggtagggg cggcctgacg
      241 tccggcccgc tgtcgagcgg attgtcgtca cggatgccta tcaggaacat cacccgtgac
      301 gccgcctcgg ccaaagcctc gggcaggggc atcctgcgcc ccgcggactc aggctcgatg
      361 ccggccagct ccgagccgta gtcggccatc accggcggct ccgcgccgaa ggcgtcgagc
      421 agggcctcgc cgctcgcgcc cctgacgccg ccccggctcg agccgagcct gcagaccgag
      481 aaggccgtcc tggacagctt atccctggtg gcggagaacg cgaacatcct gcagatcagc
      541 ggccgcgcct catagacgcc gcaatggtac ggcgtgtcgg tcctgtacag cgggcaacct
      601 tggcggccgt cgggatagga acggaccccg agccggccgg cggccgcctc ataggccttg
      661 ggccggtccg ccgcggccag gaaggcggcc aggtaggcgg cctcgacagg caggatatcc
      721 ggcacgaagc cctcacagca ggacccgcag ccaaaggggc agctgacgcc ggaggcggca
      781 acgaaggcgt cgacggccga atcggcccgc ttgtacaggt aggccaggct ctcgagccgc
      841 tccgcgagca gcgacggggg gaggacgctc ggaatgtcca tagaaggctc cgggacggtg
      901 gcgccggcct gaacgaggcc gggtccagcc gcgtatcgac gatgccggcg atccgagcgg
      961 cgggtggtac tccgtcgggg gcgactatag cgagcggcgg ctcgggcggt caagtcggcc
     1021 gctacgctcc ccgccggcct cccgagcccg acagtcttga gacgacgatg gccgccgccc
     1081 ggcgtgccgc gcgcgccagc ggccgcctgc aggccagccc gacgacgaag ccgcccatat
     1141 ctgccgccat gtcgagcagc tccgacgagc ggccgtcgac ggccagctgg agcgcctcgg
     1201 tcagggccga gaagataagt gcggcgacca gcgccaccgc tacccgtcgt cggccatcga
     1261 ggccgaagtc gagctgggcc gccagcgcca gcccgaagaa caggaccgcg tgcacgagct
     1321 tgtcgaggcc gggcacgccg gggccctcgg gcagggccga gccgggcagc agcacggcga
     1381 cgagtatcag ggccgcggtc gcggtggtcg ggaacgggaa tcgcctgggc atggcctatg
     1441 atcgcccggg accgacgacc tcgtccagcc cggcggtccc gccggccgcc gacgaggacg
     1501 ccgcgacgcc catcgccgaa gccgcccggg acgccgcgtc ccgcacggcc tcggcggtct
     1561 tgccgtcgag ccccgaacgg gcggcgatgt acgaaggctc ggcctcggcc acggcggcca
     1621 gcgagccgaa ggcgtccatg atacgccgcg ccctcgtcgg cccgacgccc ttgaccgcct
     1681 ccagcaccgg gaagcctagc gccttgccgc gcagccgctg gttcatacca gtcgcgaagc
     1741 gatgcgcctc gtcgcgcacc gcgaccacga gcctgagcgc cggcgagtcc ttgggcagca
     1801 cgaccggttc cctggcgccg tggggccaca gctcctcgtt ctccttggcc aggccgacga
     1861 cc
//
LOCUS       DAOOLN010000346         1853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_79914_length_1853_cov_2.647386, whole genome shotgun sequence.
ACCESSION   DAOOLN010000346 DAOOLN010000000
VERSION     DAOOLN010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79914_length_1853_cov_2.647386"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PLW80_05180"
     CDS             complement(<1..540)
                     /locus_tag="PLW80_05180"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPB65931.1"
                     /translation="MILGLTGGYCAGKSSAAAALHEAGWTVIDVDALGHAALERSAPE
                     VARLLGPGALKADGAPDRRAIGAMVFADPVLLARYEAIVHPPMNALVEEAVSRAGPRA
                     CVDAAILYRLPIVAACDTIIEARAPLALRLIRGRARDGLGALAMLGRIRSQRPLRDAG
                     KAWKGKTVVVWNALGEDGFK"
     gene            complement(537..>1853)
                     /gene="polA"
                     /locus_tag="PLW80_05185"
     CDS             complement(537..>1853)
                     /gene="polA"
                     /locus_tag="PLW80_05185"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPB65932.1"
                     /translation="SAPIAKAAPYCAEDAYMTLRLKEALERELADAGLSELFAGVEMP
                     VLSLLARMEREGILVDAASLEAFGKELDGELERVKAETFGLVGHEFNMNSPKQLQEIL
                     FVERKLSTGKKTKTGYSTDISVLEELAREDRVPELILRHRGLQKLRSTYVDALLALAA
                     DDPRIRTHFMQTGTATGRLSSRDPNLQNIPVREEEGRRIRKAFVAAPGKKLVSADYSQ
                     IELVVFAHLSGDPELKKAFIDGADVHRRTAALILGKAEDEVQQSERRAAKTINFGVIY
                     GMGAFRLAQELGIPRGDAQRFIDAYFERYAGVASFIRDTVDAARRDGYVSTLLGRRRM
                     IRAIDSRNANERQGAERVAVNTPIQGSAADIVKLAMLRVDSTLRERFPAARILLQVHD
                     ELIIEAPEAEADAVAAAVADAMAKAYTLSVPLRVGVEIAYSWGDMH"
BASE COUNT          258 a          637 c          622 g          336 t
ORIGIN      
        1 cttgaagcca tcctcgccta gggcgttcca gacgacgacg gtcttgccct tccaggcctt
       61 cccggcgtcg cggagcggcc tctggcttcg tatgcgcccc agcatggcga gcgcgccgag
      121 gccgtctctg gctctgcccc tgatcaggcg tagggcgagg ggcgcgcgcg cctctatgat
      181 ggtatcgcag gctgcgacta tgggtaagcg atagagtatc gccgcgtcga cgcaggccct
      241 ggggccggcc cgactcacgg cttcctcgac gagcgcgttc atcggcgggt ggactatggc
      301 ctcgtagcgg gcgagcagga ccggatccgc gaagaccatc gcgcctatcg cccggcgatc
      361 gggcgcgccg tcggccttga gcgcccctgg cccgagcagg cgcgccacct cgggcgccga
      421 tcgttcgagc gcggcgtggc cgagcgcgtc gacgtctatg accgtccagc cggcttcatg
      481 cagggctgcc gccgcgctgc tcttgcctgc gcagtagccg ccggtcagtc ccagtatcaa
      541 tgcatgtccc cccacgagta ggcgatctcg actccgaccc gtagcggcac cgatagcgta
      601 taggccttcg ccatggcgtc cgccaccgcg gcggccaccg cgtccgcctc ggcctcgggc
      661 gcctcgatga tcagctcgtc gtgtacctgg agcagtatcc tggccgccgg gaagcgctcc
      721 ctgagcgtcg agtcgaccct gagcatcgcc agcttgacga tatccgcggc agagccctgg
      781 atcggcgtgt tcaccgcgac gcgttcggcg ccctggcgct cgttggcgtt gcggctgtcg
      841 atcgccctga tcatgcggcg tcgcccgagc agggtggaca cgtagccgtc ccggcgcgcc
      901 gcgtcgacgg tgtcccgtat gaaggaggcg acgcccgcgt agcgctcgaa gtacgcgtcg
      961 atgaagcgtt gcgcgtcgcc tcgcggtatg cccagctcct gggccagcct gaaggcgccc
     1021 atgccgtaga tcacgccgaa gttgatggtc ttggccgctc gccgctccga ctgctggacc
     1081 tcgtcctcgg ccttgcccag gatcagcgcg gcggtgcgcc ggtgcacgtc ggcgccgtct
     1141 atgaaggcct tcttgagctc gggatcgccc gacaagtggg cgaacacgac cagctcgatc
     1201 tgcgagtaat cggccgagac gagtttcttg ccgggcgccg ccacgaaagc cttgcgtatc
     1261 ctgcgccctt cctcctcgcg gaccggtatg ttctgtagat tggggtcgcg gctggatagg
     1321 cgcccggtcg ccgtaccggt ctgcatgaaa tgcgtgcgga tgcgcgggtc gtcggccgcc
     1381 agcgccagca aggcgtcgac gtaggtactc ctgagcttct ggaggcctcg gtgccggagg
     1441 atcagctcgg gcacccggtc ctcgcgcgcc agctcctcga gcaccgatat gtcggtcgag
     1501 tagcccgtct tcgtcttctt gccggtggac agcttgcgct cgacgaacag aatctcttgg
     1561 agctgcttcg gggagttcat gttgaactcg tggccgacga ggccgaaggt ctcggccttg
     1621 acccgctcga gttcaccgtc cagctccttg ccgaaggctt cgagcgaggc cgcgtcgacg
     1681 agtatgccct cgcgttccat cctggccagg agcgacagta ccggcatctc gactccggcg
     1741 aacagctccg ataggccggc gtcagccagc tcgcgctcga gcgcttcctt gagcctgagc
     1801 gtcatgtagg cgtcctcggc gcagtacggg gcggctttgg ctatcggcgc cga
//
LOCUS       DAOOLN010000347         1851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_80040_length_1851_cov_2.767261, whole genome shotgun sequence.
ACCESSION   DAOOLN010000347 DAOOLN010000000
VERSION     DAOOLN010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80040_length_1851_cov_2.767261"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PLW80_05190"
     CDS             complement(<1..264)
                     /locus_tag="PLW80_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPB65933.1"
                     /translation="MTLNAFAEARRFEAVVAVLDRHGRDPSKLIPILQAVQDEYRYLP
                     EEIMTYVATALDVSPAKVYGVATFYGHFSLEPKGKHVLKICDGT"
     gene            415..1071
                     /gene="pcp"
                     /locus_tag="PLW80_05195"
     CDS             415..1071
                     /gene="pcp"
                     /locus_tag="PLW80_05195"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: protein motif:HMM:NF009676.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HPB65934.1"
                     /translation="MRVLITGFEPFGDEAINPAWEAVKRLPDTISGASIVKLLVPTAF
                     GACGAILYEALERHAPDAIVGVGQAGGRCDLSVERVAINVADGRIPDNEGRLPVDEPI
                     QPDGPAAYFSTLPIKAIVARVRERGVPASVSNSAGTYVCNYLMYLALHAVATGRPGTG
                     PGTLAGFVHVPYLPAQAVDKPATTPTMPVETMAAALEAAVEAIVEPGGATTAPEGTIC
                     "
     gene            complement(1194..1832)
                     /locus_tag="PLW80_05200"
     CDS             complement(1194..1832)
                     /locus_tag="PLW80_05200"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HPB65935.1"
                     /translation="MRSAIAAAAPAMDADDDETAYAISTIFAFNVAAVLIFPYLGRFL
                     GLSPEGFGVWAGTAVNDTSSVVAVGMAFGPEALEVATVVKLTRTLFIVPIVLALSART
                     ARLARRSSSAATGPRAESALGRSASAFPWFVIGFVVASALGTSGIVPADAASALASIG
                     KALVLVAMAGVGLGVKPAMFRAAGWRPLALGLACWAAVAASSLAAQAALGRW"
BASE COUNT          317 a          642 c          623 g          269 t
ORIGIN      
        1 cgtgccgtca cagatcttga gtacgtgctt gcccttgggt tctagggaga agtggccgta
       61 gaaggtagcc accccgtaga ccttggccgg cgatacatcg agggccgtgg ccacgtaggt
      121 catgatctcc tcgggtaggt agcggtactc gtcctgcacg gcctgcagga tcggtatcag
      181 cttggacgga tcccggccgt ggcggtcgag cacggccacc accgcctcga agcgcctggc
      241 ctccgcgaag gcgttcaacg tcatctatca ctccttatgt tctcgccggc cgccgctcgg
      301 ccgcggctcg tcggtggtat tcttgatacg tcttatcgat atatttttta ctatacaatc
      361 gacgatcatg tcaagcgagg atggatgcta tacttgcggc gaggagcgtt agcgatgcgc
      421 gtactgatta ccggcttcga gccgttcggc gacgaggcga tcaatccggc ctgggaggcg
      481 gtcaagcggc tgcccgatac gatctcgggc gcctctatcg tcaagctact cgtgccgacg
      541 gccttcggcg cctgcggagc catcctgtac gaggcgctcg agcgccacgc cccggacgcc
      601 atagtaggcg tcgggcaggc cggcggccgt tgcgatctat cggtggagcg cgtcgcgatc
      661 aacgtggccg acggccgtat ccccgacaac gaggggcggc taccggtgga cgagcctatc
      721 cagcccgacg gccccgcggc ctacttctcg acgctgccga tcaaggccat cgtcgccagg
      781 gtccgcgagc ggggcgtccc ggccagcgtg tcgaacagcg cgggcaccta cgtctgcaac
      841 tacctgatgt acctggcgct gcacgccgtg gcgacgggtc gcccgggcac gggtccgggc
      901 acgctcgccg gcttcgtcca cgtgccgtac ctaccggccc aagccgtcga caagccggcg
      961 accacgccga cgatgccagt cgagaccatg gccgccgcgc tcgaggctgc cgtcgaggcc
     1021 atcgtcgagc ccggcggcgc aacgaccgcg cccgagggaa cgatctgctg agacggccgg
     1081 tcgggctacc acctgccgag cgccgcctgc gcgatcgaca ccttaggccc ggtcccgggc
     1141 taccacctgc cgagcgccgc ctgcgcgatc gacaccttag gcccggcccc gagctaccac
     1201 ctgccgagcg ccgcctgcgc ggcgagcgag gaggccgcga ccgcggccca gcaggccagg
     1261 ccaagcgcca ggggtctcca gccggcggcc cggaacatgg ccggcttgac gccgaggccg
     1321 accccggcca tagccacgag gacgagggcc ttaccgatcg aggcgagcgc cgaagcggcg
     1381 tcggccggca cgatacccga cgtaccgagc gcggatgcca cgacgaagcc tatgacgaac
     1441 caggggaacg ccgaagcgct ccgaccgagc gctgactccg cgcgcggccc cgtagccgcc
     1501 gaggaggaac gccgcgcgag cctggccgtc cgggccgata gcgccagtac gatcggaacg
     1561 atgaagagcg tacgcgtgag cttgacgacg gtcgcgacct cgagcgcctc gggcccgaac
     1621 gccatgccga cagcgacgac cgacgaggta tcgttgaccg cggtgccggc ccagacgccg
     1681 aagccctcgg ggctcaaacc caggaaccgt cccaggtagg ggaagataag cacggcagcc
     1741 acattgaagg cgaaaatggt cgatatggcg tacgccgtct cgtcgtcgtc cgcgtccatc
     1801 gccggagccg cggcggctat cgccgatcgc acgggtcacg acgtggatgt g
//
LOCUS       DAOOLN010000348         1846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_80371_length_1846_cov_2.970408, whole genome shotgun sequence.
ACCESSION   DAOOLN010000348 DAOOLN010000000
VERSION     DAOOLN010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80371_length_1846_cov_2.970408"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PLW80_05205"
     CDS             <1..377
                     /locus_tag="PLW80_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPB65936.1"
                     /translation="GISSIASLLDGAVKTGVADKKGAWYTYGEEKLGQGRENAKATLE
                     TNQELADEIEAKVRAIMFPGKSPEAAGGKKPAAGTKAPSPATVPAGTASAQPPRPQPA
                     AKAVPTDGRAARPAVDEDGGLF"
     gene            389..895
                     /gene="folK"
                     /locus_tag="PLW80_05210"
     CDS             389..895
                     /gene="folK"
                     /locus_tag="PLW80_05210"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPB65937.1"
                     /translation="MGLGSNVGDSLAILRGALEALGAFLSGLRSSSVYRSAPRYVVDQ
                     ADFLNLVALGETDLAPAELLTRTQAVEASFGRDRTKERRKGPRSLDVDVLLYGDLVID
                     LPELLVPHPGMTERAFVLVPLAELSPALVDPRDGQPFSHRLGDVADQGIYLHAGPPLY
                     CRNDAWKQ"
     gene            883..1653
                     /locus_tag="PLW80_05215"
     CDS             883..1653
                     /locus_tag="PLW80_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HPB65938.1"
                     /translation="MEAINEASLEPERAGRSYRLAEFEGPLDLLLFLIKKNEVNIYDI
                     PIASITEQFIASLSEAEAADLDDLTEFYMLAATLLYIKSRMLLPVEMNLADEIEDPRR
                     DLVEKLIEYQKFKKLSELMEKKELEVEWTIERTKMQRPLPFGDEELWDKIDVWDLLKS
                     FSGLMGGMSSERIIDMYEEVSINEKTALIHELLESRESFGFHDLVTRSGSLLDIVCAF
                     LAVLEAVKYRVISIHQHRLFGDIQIRRHERRGTDGTEP"
     gene            1637..>1846
                     /locus_tag="PLW80_05220"
     CDS             1637..>1846
                     /locus_tag="PLW80_05220"
                     /inference="COORDINATES: protein motif:HMM:NF016005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPB65939.1"
                     /translation="MELNLDREAAILETILFLESEPVDKAALSRISGLGIDVTEAALD
                     HLRECLASACHGIELVEMGGGWLLVP"
BASE COUNT          340 a          617 c          587 g          302 t
ORIGIN      
        1 agggcatctc gtcgatagcc agcctgcttg acggcgcggt caagaccggc gtcgccgaca
       61 agaagggcgc ctggtatacc tacggcgagg agaagctcgg gcagggccgc gagaacgcca
      121 aggccacgct cgagaccaac caggagctgg cggacgagat cgaggccaag gttcgcgcca
      181 tcatgttccc cggtaagtcg cccgaggcgg ccggcggcaa gaaaccggcc gcgggaacca
      241 aggctccgtc tccggcgacc gttccggccg gtaccgcgtc ggcgcaaccg cccaggccgc
      301 agccggccgc caaggctgtg ccgaccgacg gccgggctgc cagaccggcc gtggacgaag
      361 acggcgggct cttctgagcc tcgtctacct ggggctcggc tcgaacgtag gcgacagcct
      421 ggccatcctg cgcggcgctc tcgaggccct gggcgctttc ctgtcgggcc tgcgctcctc
      481 gtcggtctac agaagcgcgc cgcgctacgt cgtcgaccag gccgacttcc tcaacctcgt
      541 cgccctcggc gagactgatt tggcgccggc cgagctcctg accaggaccc aggccgtcga
      601 ggcgagcttc gggcgagacc ggaccaagga gcgccgcaag gggccgcgca gcctcgacgt
      661 cgacgtcctc ctgtacggcg acctcgtcat cgatttgccc gagctgctcg tgccgcatcc
      721 tggcatgacg gagcgcgcct tcgtgctcgt gccgctcgcg gagctgtcgc cggccctcgt
      781 cgaccctcgg gacgggcagc cgttctccca ccgcctcggc gacgtggccg accaggggat
      841 ttacctgcac gccggcccgc cgctatactg ccggaacgac gcatggaagc aataaacgaa
      901 gcatcgctcg agcccgagcg agctgggcgt agctatcgcc tggccgaatt cgagggaccc
      961 ctcgacctcc tgctcttcct catcaagaag aacgaggtca atatctacga tatacctata
     1021 gcctcgatca ccgagcagtt catcgcctct ctgtccgagg ccgaggccgc cgacctggac
     1081 gacctgaccg agttctatat gctcgcggcg accctgctgt acatcaagag ccgtatgctc
     1141 ctgccggtcg agatgaacct ggccgacgag atcgaggatc cgcggcgcga cctcgtcgag
     1201 aagctgatcg agtaccagaa gttcaagaag ctatccgaac tcatggaaaa gaaagagctc
     1261 gaggtcgagt ggaccataga gcggaccaag atgcaacggc ccttgccctt cggcgacgag
     1321 gagctatggg acaagatcga cgtctgggac ctactcaagt cgttctccgg cctgatgggc
     1381 ggcatgtcga gcgagcgcat catcgacatg tacgaggagg tgtcgatcaa cgagaagacg
     1441 gcgctgatcc atgagctgct cgagtccagg gaatccttcg gcttccacga cctggtcacg
     1501 cgctcgggtt ccctgctcga catcgtctgc gccttcctgg ccgtgcttga ggcggtcaag
     1561 taccgcgtca tatcgataca ccagcatcgc ctgttcggcg atatccagat acgtaggcac
     1621 gagaggcggg gcaccgatgg aactgaacct tgatcgcgag gccgccatcc tcgagacgat
     1681 actcttcctt gagtccgagc ctgtcgacaa ggcagcgctc tcgcggataa gcggcctggg
     1741 tatcgacgtg acggaggcgg ccctcgacca cctgcgcgaa tgcctggctt cggcctgtca
     1801 cggcatcgaa ctcgtcgaga tgggcggcgg ctggctcctc gtcccg
//
LOCUS       DAOOLN010000349         1846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_80382_length_1846_cov_2.650475, whole genome shotgun sequence.
ACCESSION   DAOOLN010000349 DAOOLN010000000
VERSION     DAOOLN010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80382_length_1846_cov_2.650475"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="PLW80_05225"
     CDS             <1..973
                     /locus_tag="PLW80_05225"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HPB65940.1"
                     /translation="AGGRYEPQAGAAPAKEFAVRELPGDGSFNERIDAYYAEHGSELS
                     RAALLERAAKYFSQRRAQLESRLEALEKAATEYADADRYRELGDILMANSGQALSGPS
                     LRAEDFFRGGEVVIRVDPSKSVVDNAKALYEKAKKARSGALETAAELDELREAVAALD
                     EERRRVEATENPYAIRAFLAKRRTAPSQGPKRFPGLSLERDGWLFLIGRSAVENDELL
                     RRHVRGNDLWFHARDYSGSYVFVKAKANKSVPLEIMLDAGSLALYYSKGRSSGAGDLY
                     YTQAKYLRRAKNGPKGLVIPTQEKNLRAKLDEGRLKELRRLIGRDGD"
     gene            complement(989..>1846)
                     /locus_tag="PLW80_05230"
     CDS             complement(989..>1846)
                     /locus_tag="PLW80_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB65941.1"
                     /translation="PLGSFIFLGPTGVGKTLLAKTLAESLFGSEDALIRIDMSDFMEK
                     HNAARLVGAPPGYVGYDEGGMLTERVRRKPYSVVLFDEIEKAHPDVFNLLLQLLEEGE
                     LRDNLGHAVSFRNCVVIMTSNAGTRELTSGARLGFGAETRRFDYDAVKSAALAELKRL
                     FNPEFINRVDQVVVFHPLDRDQVRVVLELELSRLRRRLAERGVSLELDRKAMDRLIRD
                     GYDEAYGARPMRRMISKLIEDPLVAIMVEGRCPPGATARVREKDGELSIRVSTPQRAP
                     SVPSAVSKD"
BASE COUNT          323 a          631 c          605 g          287 t
ORIGIN      
        1 ggcgggcggg cgctacgagc cccaagccgg agcggcgccg gccaaggagt tcgccgtgcg
       61 cgagctgccc ggcgatggct ccttcaacga gcgcatcgat gcctactacg ccgagcacgg
      121 ctcggagcta tcccgggccg cgctgctcga acgcgcggcc aagtacttca gccaacggcg
      181 cgcccagctc gagtcgcgtt tagaggccct ggagaaggcg gccaccgagt acgccgacgc
      241 cgaccgctac cgcgagctgg gagatatcct gatggccaac tcgggccagg cgctatcggg
      301 tccgtcgctc agagcggaag acttcttccg cggtggcgag gtggtcatac gcgtcgatcc
      361 atccaagagc gttgtcgaca acgccaaggc cctctacgag aaggccaaga aggcccgctc
      421 tggcgccctg gagacggccg ccgagctcga cgagctacgc gaagccgtcg cggcgctcga
      481 tgaagagcga cggcgcgtcg aggccaccga gaacccttac gctatccgcg ccttcctggc
      541 caagcggagg accgccccgt cccaggggcc gaagcgcttc ccgggactgt cgctcgagcg
      601 cgacggctgg ctgttcctga tcggccgctc ggcggtcgag aacgacgaac tcctgcgccg
      661 ccatgtccgc ggcaacgacc tgtggttcca tgcgcgcgac tactcgggat cgtacgtgtt
      721 cgtcaaggcc aaggcgaaca agagcgtgcc gctcgagatc atgctcgacg ccggctccct
      781 ggccctgtac tactcgaagg gccgctcatc cggcgccggc gacctatact acacgcaggc
      841 caagtacctc cggcgggcca agaacggccc caagggcctg gttatcccga cccaggagaa
      901 gaacctgcgc gccaagctcg acgagggacg gctcaaggag ctcaggcgcc tcatcggccg
      961 ggacggcgac taacctcggt ggcgaggctc agtccttgga gacggcgctc ggtacggacg
     1021 gcgcgcgttg cggcgtcgac accctgatcg acagctcgcc atccttctcg cgcaccctcg
     1081 ctgtcgcccc gggagggcag cggccctcga ccatgatggc cacgagcgga tcctcgatga
     1141 gcttggagat catgcgccgc atcggcctgg ctccgtaggc ctcgtcgtag ccgtcgcgga
     1201 tcagccgatc catcgccttg cggtcgagct ccagcgagac gccacgctcg gcgaggcgcc
     1261 tacgtagcct ggacagctcc agctcgagca cgacgcgcac ctggtcgcgg tcgagcgggt
     1321 ggaagacgac cacctggtcg acgcggttga tgaattccgg gttgaataga cgcttaagct
     1381 cggccagcgc cgccgacttg acggcatcgt agtcgaaacg cctcgtctcg gccccgaagc
     1441 ccagcctagc cccgctcgtc agctcgcggg tgccggcgtt gctggtcatg atcacgacgc
     1501 agttcctgaa tgaaacggcg tgtcccaggt tatcgcgcag ttcgccctcc tcgagcaact
     1561 gcaggagcag gttgaacacg tcaggatggg ccttctcgat ctcgtcgaac agcaccacgc
     1621 tatacggctt gcgtctgacc cgttcggtca acatgccgcc ctcgtcgtag ccgacatagc
     1681 ccggaggcgc gccgacgagc ctggcagcgt tatgcttttc catgaagtcc gacatgtcga
     1741 tgcggatcag cgcgtcctcg ctgccgaaca ggctctcggc gagggtcttg gccagtaggg
     1801 tcttgccgac gccggtcggt ccgaggaaga tgaaggagcc gagcgg
//
LOCUS       DAOOLN010000350         1845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_80444_length_1845_cov_2.576536, whole genome shotgun sequence.
ACCESSION   DAOOLN010000350 DAOOLN010000000
VERSION     DAOOLN010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80444_length_1845_cov_2.576536"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..895)
                     /gene="tsf"
                     /locus_tag="PLW80_05235"
     CDS             complement(41..895)
                     /gene="tsf"
                     /locus_tag="PLW80_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPB65942.1"
                     /translation="MDIKASDIKNLREKTGAGMMDCKKALVEAEGDFNLAERKLKEWG
                     LAGVAKRSGRATNEGRVFIAEGADAIAVVEIACETDFVCRNDDFIAGGDRMAKLALAK
                     GYASANDELEAIVKDMASVIKENITLKSVTLVKAGAAEYVHSYVHGEGKLAVVVKMRA
                     DKAEAFKNASVTGFVHDLALHVAAFRPMFVDQGRIGADWIKEQQEIFQKQVEGDEKMV
                     GKPTKVIEGILAGKLKKLMSEICLVDQGFVKDEKQTVAAVMASIAKAAGTQLSIVDYA
                     YIKAGDNQ"
     gene            complement(906..1781)
                     /gene="rpsB"
                     /locus_tag="PLW80_05240"
     CDS             complement(906..1781)
                     /gene="rpsB"
                     /locus_tag="PLW80_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPB65943.1"
                     /translation="MAVVTMKSLLESGVHFGHQVKRWDPRMKKFIFAERNGIHIIDLQ
                     KTIQSIKEAYDVVRKTVASGKPVLFVGTKKQAQQAIQKEAERCEQFYVNNRWLGGMLT
                     NFATVRKSIERLIKLEKEDFSSFTKKEQAQLQKEKAKLEKNLSGIKGMTKLPGVIFVI
                     DTRKEAIAVAEAQRLGIPIVAVVDTNCNPEGIDYPIPGNDDAIRSISLFTQIIANAVV
                     EAESETGLKIIETLQDEDGAEEEAKAAVEDEEEEVEVDPATYSAEPAASAASEVAAEG
                     AAEEPIVEEDKYYEK"
BASE COUNT          275 a          583 c          555 g          432 t
ORIGIN      
        1 cgaagccgcc ccggccgcga ggctggggcg gccatggacc ttactggttg tccccggcct
       61 tgatgtaggc gtagtcgacg atcgacagct gcgttccggc ggccttggcg atcgaggcca
      121 tcaccgcggc cacggtctgc ttctcgtcct tgacgaagcc ctggtcgacc aggcagatct
      181 cgctcatcag cttcttaagc ttgccggcga ggatgccctc gatgaccttg gtaggcttgc
      241 cgaccatctt ctcgtcgccc tcgacctgct tctggaagat ctcctgctgc tccttgatcc
      301 agtcggcgcc gatgcgtccc tggtcgacga acattggcct gaaggccgcc acgtgcaggg
      361 ccaggtcatg gacaaacccg gttaccgaag cgttcttgaa cgcctcggcc ttgtcagccc
      421 tcatcttgac gacgacggcg agcttgccct cgccgtggac gtaggagtgg acgtactcgg
      481 cggcgccggc cttgaccagc gtgacgctct tgagcgtgat gttctccttg atgacgctcg
      541 ccatatcctt gacgatggcc tcgagctcgt cgttggccga cgcatagccc ttagcgaggg
      601 caagcttggc catgcgatcg ccacctgcga tgaagtcatc gttgcggcac acgaagtcgg
      661 tctcgcaggc gatctcgacg accgcgatgg cgtcggcgcc ctcggcgata aagacgcggc
      721 cctcgttggt ggcgcggccg gagcgcttgg cgacgcccgc gaggccccac tccttgagct
      781 tccgctcggc cagattgaag tcgccctcgg cttctacgag agctttcttg cagtccatca
      841 tacccgcgcc ggttttctcg cgcaggttct ttatatcgct agcctttata tccacggtat
      901 gctccttatt tctcgtagta cttgtcttcc tcgacgatgg gctcctcggc ggcgccttcg
      961 gcggctacct cggaagcggc cgaggcggcc ggttcggcgc tgtaggtagc cgggtcgacc
     1021 tcgacttctt cctcttcgtc ctctaccgcg gccttggcct cttcctcggc gccatcctcg
     1081 tcctgcaggg tctcgatgat cttgaggccg gtctcgctct cggcctcgac gaccgcattg
     1141 gcgatgatct gcgtgaacag ggagatcgag cggatggcgt cgtcgtttcc ggggatgggg
     1201 taatcgatgc cctcggggtt gcagttggta tcgacgacgg cgacgatcgg gatccccagg
     1261 cgctgggcct cggctacggc gatggcttcc ttgcgggtgt cgatcacgaa gatgacgcca
     1321 gggagcttgg tcatgccctt gatgccggaa aggttcttct cgagcttggc cttctccttc
     1381 tggagctggg cttgttcttt cttggtgaag ctagagaagt cttccttctc gagcttgatc
     1441 aggcgctcga tcgactttcg tacggtcgcg aagttggtga gcatgccgcc cagccaacga
     1501 ttgttcacat agaactgctc gcagcgctcg gcttccttct gaatggcctg ctgggcctgt
     1561 ttcttggtgc cgacgaacaa caccggcttg ccggacgcga ccgtcttgcg gaccacgtca
     1621 taggcttcct taatcgactg gatcgtcttc tgcagatcga tgatatggat gccgttccgc
     1681 tcggcgaaga taaatttctt catgcgcgga tcccagcgct tgacctggtg cccgaaatgg
     1741 actccggact ccaaaaggct cttcatggtg actacggcca cgtgttcctc catcggcccc
     1801 cgcgttcggg cgggagcccc cttcgcttgt tctcgtcgat atgga
//
LOCUS       DAOOLN010000351         1844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_80519_length_1844_cov_1.911683, whole genome shotgun sequence.
ACCESSION   DAOOLN010000351 DAOOLN010000000
VERSION     DAOOLN010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80519_length_1844_cov_1.911683"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PLW80_05245"
     CDS             complement(<1..311)
                     /locus_tag="PLW80_05245"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPB65944.1"
                     /translation="MDMIEAMAERHSVRSYTSTPIEGDVLEALAASIAEINKASGLAV
                     ELAPEGGKAFDNLLGRYGKFRGVRGLFVLKGRRSKDLGLRAGYWGERLVLEATALGLG
                     T"
     gene            complement(337..882)
                     /locus_tag="PLW80_05250"
     CDS             complement(337..882)
                     /locus_tag="PLW80_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HPB65945.1"
                     /translation="MDAERRGALLVILRDRFESNIQRHAGVSWLDVLERLEARPAALD
                     ALGAMEASGGEPDLVAVDGGSGAFVFFDCSPESPVGRRSLCYDRAALDERKEHRPAGD
                     TVSAAASMGAELLTEDEYRYLQGLGEFDLKTSSWIRTPEPVRALGGALFMDRRYGRVF
                     VYHNGASSYYGVRGFRASVRA"
     gene            complement(872..1252)
                     /locus_tag="PLW80_05255"
     CDS             complement(872..1252)
                     /locus_tag="PLW80_05255"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HPB65946.1"
                     /translation="MRDKPVDVAAYFERFDEPVRRRLEQIRSLVFELLPEATEAIKYG
                     IPTFIHNGNLVHYAGYTGHIGLYPAPSGLERFGDELARYKRGKGSVRFPLDEELPIEL
                     IRRIVAFRIEEQGGRPRKDGDDGR"
     gene            complement(1275..>1844)
                     /locus_tag="PLW80_05260"
     CDS             complement(1275..>1844)
                     /locus_tag="PLW80_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HPB65947.1"
                     /translation="IEVLILVEAFGMEQTLGNVMFATTFIVTDILSENLGKAASKKAV
                     SIGIVTSVSFIVVTQLWLAFSPSPNDWAFESVRTLFSNTPRLMLVSLLVYAVAQRFDV
                     WAYHAIWAVTTRVLGDREKGLWIRNNGSTLVSQLLNTVLYSAGAFWGRYPARTLLSIM
                     ASSYLIYVATSLLDTPVVYLARRMHRRGV"
BASE COUNT          324 a          599 c          599 g          322 t
ORIGIN      
        1 caggtgccga ggcctagggc ggtggcctcg aggacgaggc gttcgcccca gtagccggcc
       61 ctaaggccga gatccttcga cctgcggcct ttcagcacga agaggccgcg gacgccgcgg
      121 aacttgccgt agcgtcctaa caggttgtcg aaggccttgc cgccctccgg cgccagctcg
      181 acggccaggc cggaagcctt atttatttcc gctatcgagg ccgcaagcgc ctcgaggacg
      241 tcgccctcga ttggcgtgct cgtatacgaa cgcacagaat gcctctcggc catcgcttcg
      301 atcatgtcca tggtagctcc tctccccgtc gtcgattcag gcgcgtacgg acgccctgaa
      361 gcccctgacg ccatagtacg acgaggcgcc attatgatag acgaagaccc gtccgtagcg
      421 cctatccatg aacagggctc cgccgagggc gcggaccggc tcaggtgtac gtatccagct
      481 cgaggtcttc aggtcgaact cgccaaggcc ctgcaggtac cggtactcgt cctcggtcag
      541 gagctcggcg cccatcgacg cggccgccga gacggtgtcg ccggccggcc tatgctcctt
      601 gcgctcgtcg agggcggcgc ggtcgtagca gaggctccgc cgccctaccg ggctctcggg
      661 cgagcaatcg aagaacacga acgcccccga gccgccgtcg accgcgacga ggtccggctc
      721 cccgcccgag gcctccatcg ctccgagcgc atcgagcgcg gcgggcctgg cttcgaggcg
      781 ctcgagcacg tcgagccacg agacgccggc atggcgctgt atgttgctct cgaatctgtc
      841 gcgcagtata acgagcagcg cgcctcgccg ttcagcgtcc atcgtcgcca tcctttcgtg
      901 gccggccgcc ctgctcctct atcctgaagg cgactatccg cctgatcaac tcgattggca
      961 gttcctcgtc cagcgggaaa cgtaccgagc ccttaccgcg cttatagcgg gcgagctcgt
     1021 cgccgaagcg ctcgaggccg ctcggcgccg gatagaggcc gatatggccc gtatagccgg
     1081 cgtaatggac gaggttgccg ttatggatga aggtcggtat gccgtacttg atggcctcgg
     1141 tggcctcggg tagcagctcg aataccagcg accgtatctg ctcgagcctg cgccgtaccg
     1201 gctcgtcgaa gcgctcgaag taggcggcca cgtccacggg tttgtccctc atcggtatgc
     1261 cgcccgtccg aatctcatac gcccctccta tgcatgcgcc tggccaggta gacgacgggc
     1321 gtatcgagca ggctcgtcgc cacgtagata agataactcg aggccatgat ggagagaagg
     1381 gtcctggccg gatagcgtcc ccagaaggcg cccgcggagt acagtacggt attgaggagt
     1441 tgcgagacga gggtggagcc gttgttcctg atccacaggc ccttctcccg gtcgccgagt
     1501 acacgggtcg tcacggccca gatggcgtgg taggcccaga cgtcgaagcg ctgggcgacg
     1561 gcgtagacga ggaggctgac gagcatgagc cgcggggtgt tcgagaacag ggtcctgacg
     1621 ctctcgaagg cccagtcgtt aggcgacggg ctgaaagcga gccacagctg ggtcacgacg
     1681 atgaagctga ccgaggtgac gatgccgatg gacacggcct tcttcgacgc ggccttgccc
     1741 aggttctcgc tcaggatgtc ggtgacgatg aaggtggtag cgaacatcac gttcccgagg
     1801 gtctgttcca tgccgaaggc ctcgacgagg atcagcacct cgat
//
LOCUS       DAOOLN010000352         1840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_80776_length_1840_cov_2.153501, whole genome shotgun sequence.
ACCESSION   DAOOLN010000352 DAOOLN010000000
VERSION     DAOOLN010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80776_length_1840_cov_2.153501"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..>525
                     /locus_tag="PLW80_05265"
     CDS             92..>525
                     /locus_tag="PLW80_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HPB65948.1"
                     /translation="MVTAILFDLDNTLYPASADMGRNTVRRMNEYAARLLGVDPEEAA
                     AVRRERMPGYGTTLEWLMAEHGFTDADDYFAYVHPDGEEDSVAFDPALGPFLDSIALP
                     KYVFTNAPMEHADRVLAKLGVADRFERVFDVRFCGLKGKPAAS"
     assembly_gap    526..535
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <536..768
                     /locus_tag="PLW80_05270"
     CDS             <536..768
                     /locus_tag="PLW80_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65949.1"
                     /translation="AVDKVLAAIGQPADSTVFLDDVPRYVRGFIDRGGRGLLVDHFDA
                     HRDSGLETIRTIYELPGRLAGGPYEKEDACRS"
     gene            756..1268
                     /locus_tag="PLW80_05275"
     CDS             756..1268
                     /locus_tag="PLW80_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65950.1"
                     /translation="MPFLSLLPDLIRKRPGRVELTVSKATRALYLGLGAILVTVLSRD
                     PSGTPFAVMFALVIGLAAVSEDRWVFDGEASEVRRRTGVLFIAKSWAVDLDMISAIEL
                     DSEYNGADQGDPYAKVSAGSPKDRCAIRLALYDGRTLVVVSAKEKRLEELRLRATAIA
                     EAIGKPLSEC"
     gene            complement(1265..1537)
                     /locus_tag="PLW80_05280"
     CDS             complement(1265..1537)
                     /locus_tag="PLW80_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65951.1"
                     /translation="MSRREPEGSGRQGPSWLRRAAAFAAGALACACFAATAFLAILSL
                     WGVVAAPFVRLRSASFVIACALAGSVAATVAAWRAALRLYRRHQVR"
     gene            complement(1534..>1840)
                     /locus_tag="PLW80_05285"
     CDS             complement(1534..>1840)
                     /locus_tag="PLW80_05285"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPB65952.1"
                     /translation="LPLALMAALCVYYFKTDELTKVQRWALCGIVGGGVGNIIDRIAR
                     PDGVIDFLSVKFYGLFGLERWPTFNVADSAVVVCGILLVVSGFFVAPKGDESERPTP"
BASE COUNT          328 a          617 c          608 g          277 t
ORIGIN      
        1 gacggaatac catgtacgcg tcaagtattt gtttattgcc gacgagggca tcgccttcca
       61 tacgtcggcg tcagcatggt atgaagtatc catggttacc gcgatactct tcgatctcga
      121 caacaccctc tacccggcga gcgccgacat gggacgcaac accgtgcggc gcatgaacga
      181 gtacgccgcc aggctactcg gcgtcgaccc ggaggaggcg gcggccgttc ggcgcgagcg
      241 catgcccggc tacggcacga cgctcgagtg gctgatggcg gagcacggct tcaccgacgc
      301 cgacgactac ttcgcctacg tccaccccga cggcgaggag gactcggtcg cattcgaccc
      361 cgccctcggc cccttcctcg actccatagc cttacccaag tacgtgttca ccaacgcgcc
      421 gatggagcac gcggaccgcg tcctcgccaa actgggcgtg gccgaccgtt tcgagcgggt
      481 cttcgacgtt cgcttctgcg ggctcaaggg caagccggcg gcctcnnnnn nnnnncggcg
      541 gtcgacaagg tgctcgccgc catcggccag ccggccgata gcaccgtatt cctcgacgac
      601 gtgccgcgat atgtccgcgg cttcatcgac cgcggcggcc gtggcctgct cgtcgaccac
      661 ttcgacgcgc atcgcgattc gggcctcgag acgatacgca cgatctacga gctgccgggc
      721 cgcctggccg gcgggccgta cgagaaggag gacgcatgcc gttcctgagc cttttacccg
      781 acctgatacg taaacgcccc gggcgcgtcg agctgacggt atccaaggcc acgagggcgc
      841 tgtacctggg cctcggcgct atactcgtga cggtattgag ccgcgacccg tccggcacgc
      901 ccttcgcggt catgttcgcc ctggtcatcg gcctggccgc cgtgtccgag gatcgatggg
      961 tgttcgacgg ggaggccagc gaggtaaggc gccggaccgg cgtcctgttc atcgcgaaga
     1021 gctgggccgt cgacctggac atgatcagcg cgatcgagct ggactccgaa tataacgggg
     1081 ccgaccaggg cgacccctat gccaaggtgt cggccggctc gcccaaggac cgctgcgcga
     1141 tcaggctggc cctatacgac ggcaggacgc tggtagtcgt ctcggccaag gagaagcggc
     1201 tcgaggagct acggcttcgc gcgacggcca tcgccgaggc gataggcaag ccgctgtcgg
     1261 agtgctagcg gacctggtgg cgcctatata gccggagcgc cgcccgccag gcggccacgg
     1321 tcgccgcgac ggaacccgcc agcgcgcagg ctatgacgaa gctggccgag cggagccgta
     1381 cgaagggcgc cgcgacgacg ccccagagcg acaggatggc taggaaggct gtagcggcga
     1441 aacaggcgca ggccagggca ccggccgcga aggcggccgc gcgccgtagc caggacggtc
     1501 cctggcggcc cgaaccttcc ggctcgcgtc ggctcaaggc gtcggccgct ccgattcgtc
     1561 gcccttgggc gctacgaaga aaccgctgac gaccagcagg atgccgcata cgacgacggc
     1621 cgaatcggcc acgttgaagg tcggccagcg ctccaggccg aacagcccgt agaatttaac
     1681 cgataggaag tcaatgacgc catcgggcct ggctatacgg tcgatgatat taccgacccc
     1741 gcctccgact atgccgcaga gggcccagcg ctggaccttc gtcagctcgt cggtcttgaa
     1801 atagtagacg cagagggccg ccatcagggc gagcggcagc
//
LOCUS       DAOOLN010000353         1837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_80949_length_1837_cov_3.039843, whole genome shotgun sequence.
ACCESSION   DAOOLN010000353 DAOOLN010000000
VERSION     DAOOLN010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80949_length_1837_cov_3.039843"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="PLW80_05290"
     CDS             <1..894
                     /locus_tag="PLW80_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HPB65953.1"
                     /translation="QMQAIIQACVETRSPVILQVSSGARKYANSNLLRNMARGAVEYA
                     AELGYKIPIVLHLDHGDSYELCVDCIESGFSSVMIDGSHLPYDENVALTRKVCEYAHS
                     RTDYVTVEGELGVLAGVEDEVSAEHSSYTQPDEVQDFVSKTGVDSLAISIGTSHGRTK
                     FKPSQCTRSADGVLIPPPLRFDILAEIEKRLPGFPIVLHGSSSVPMEYVRIIEQFGGK
                     LSDSVGIPEEQLRKAAKSAVCKINIDSDGRLAMTAMIRKVFAEKPDEFDPRKYLGPAR
                     DELKKLYMHKNKEVLGSAGQA"
     gene            1006..>1837
                     /locus_tag="PLW80_05295"
     CDS             1006..>1837
                     /locus_tag="PLW80_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65954.1"
                     /translation="MSKRILACLAMATAMVASAAADDELAFGVEGVRVFSGFIPSGGD
                     VSLTYTGFQLYEPGVTKLYLEAGGGYEDRSIVRDLMTGDPYVADASGYEYRSPNFQWE
                     LAFIQGLSRRDDGNNLVEAFAYYRGRYDDYLTEADGLSDAVFADIGGLFGTSVMGGLS
                     YDSVSTSGHKQKTGLYAEATAEWAPGFLNALTDFWRVSAQARYFVPLYDMESDGGNLL
                     NLYAGFFAGVDYADGGSVPIYVNQSFGGRNLRGSVGDCVRGYGWNKFDSSLKAASSAE
                     L"
BASE COUNT          347 a          594 c          572 g          324 t
ORIGIN      
        1 cagatgcagg cgatcatcca ggcctgcgtc gagacgagga gccccgtgat cctgcaggtc
       61 tcctcgggcg cccgcaagta cgccaactcc aacctgctgc gcaatatggc gcgaggcgcc
      121 gtcgagtacg cggccgagct cggctacaag atcccgatcg tcctccacct cgaccacggc
      181 gacagctacg agctatgcgt cgactgtatc gagagcggct tctcgagcgt gatgatcgac
      241 ggctcgcacc tgccctacga cgagaacgtc gccctgacca ggaaggtctg cgaatatgcc
      301 cattcgagga ccgattacgt gaccgtcgag ggcgagctcg gcgttctggc cggcgtcgag
      361 gacgaggtgt cggccgagca ttcgagctat acccagcccg atgaggttca ggacttcgtc
      421 tccaagaccg gcgtcgactc cctggccatt tcgatcggca caagccatgg gcgcacgaag
      481 tttaagccgt cgcagtgcac caggagcgcc gacggcgtgc tgatcccgcc gcccctgcgc
      541 ttcgacatac tcgccgagat cgagaagcgt ctgcctggtt tcccgatcgt gctccatggc
      601 tcgtcgagcg tgccgatgga gtacgtcagg atcatcgagc agttcggcgg caagctgtcc
      661 gactcggtcg gcatccccga ggagcagctg cgcaaggccg ccaagagcgc cgtctgcaag
      721 atcaacatcg actccgacgg ccgcctcgcc atgaccgcga tgatacgcaa ggtcttcgcc
      781 gagaagccgg acgagttcga cccgcgcaag tatctcgggc cggcccgcga cgaactcaag
      841 aagctgtaca tgcacaagaa taaggaagtg ctcggatcgg ccggacaggc ctaaacgagg
      901 aatcgccata gtcgaggggc ccgccatggg tccctctttt ttttgccgaa tcgcgccgta
      961 gctggtatta tggcgcaata tcgatagcgt tcttggagga tcacgatgag taaacgcata
     1021 ctagcgtgcc tggccatggc gacagccatg gtcgcatcag ccgcggcgga cgatgagctg
     1081 gccttcggcg tcgagggcgt gcgcgtcttc agcggcttca taccgtcggg aggcgacgta
     1141 tcgctgacct ataccggttt tcagctctac gagccgggcg taacgaagct gtacctcgag
     1201 gccggcggcg gctacgagga caggtccatc gtccgcgatc tgatgactgg cgacccctac
     1261 gtcgccgacg cctccgggta cgagtaccga tcgcctaatt tccagtggga gctggccttc
     1321 atccaaggcc tgtccaggcg cgacgatggc aacaacctcg tcgaggcctt cgcgtactac
     1381 cgcggccgct acgacgacta cctgaccgag gccgacggcc tgtccgatgc ggtgttcgcc
     1441 gacataggcg gcctgttcgg cacatcggtc atgggcggcc tgtcctacga ctccgtttcg
     1501 acgagcggcc acaagcagaa aaccgggcta tacgccgagg ctaccgccga atgggcgccg
     1561 ggcttcctga acgcgctgac cgatttctgg agggtctcgg cccaggcgcg ctacttcgtc
     1621 cccctgtacg acatggaaag cgacggcggc aacctgctca acctgtatgc cggcttcttc
     1681 gccggagtcg actacgccga tggcgggtcg gtgcccatct acgtgaacca gagcttcggc
     1741 ggacgtaacc tacgcggctc ggtcggcgac tgcgtacgcg gctacggctg gaacaagttc
     1801 gactcgtcgc tcaaggctgc ctcgagcgcc gagctca
//
LOCUS       DAOOLN010000354         1836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_81024_length_1836_cov_2.872544, whole genome shotgun sequence.
ACCESSION   DAOOLN010000354 DAOOLN010000000
VERSION     DAOOLN010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81024_length_1836_cov_2.872544"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="PLW80_05300"
     CDS             complement(<1..995)
                     /locus_tag="PLW80_05300"
                     /inference="COORDINATES: protein motif:HMM:NF036553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5312 family protein"
                     /protein_id="HPB65955.1"
                     /translation="MASASTFEALVLDLSPSERSEMLERLQRSCTVSTEPLFERGHLQ
                     EPRIDYRAAYSELGLVTRIIITLRSIFGGGSKEDLVKERFLKAIVKRVESTSAGLIDA
                     KRRVLQEAFYRELATLRQAARYFYDLLDRTLEKNRAPFFAFLASLEFEEIHRELSTDA
                     DPYLVAEQNSTMLDSDVRIAVLAAMESALGRIDEDKRRLMYKDVRNLHVLKSLSSFLF
                     DRMLAAFQTNVSGLKELSMYAAAEQLAELAAILCSLDQPPSARLMEAILGFELNDDVG
                     REGFDLDEAVTTELASAEKALAAIRGFNARVPIEDILKVANEDPNWQCATTGGGE"
     gene            complement(1025..>1836)
                     /locus_tag="PLW80_05305"
     CDS             complement(1025..>1836)
                     /locus_tag="PLW80_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPB65956.1"
                     /translation="FDPIAEPRVVYEGDGVIVVAKPSGMHCAPAGRLAPAGPGSTLSD
                     WLFERYPMAAAVAGRGRGEGGLLHRLDGATSGLVAFATDDGSFAAMSAAAARGAFVKT
                     YRALCLPAPVGLAGSRPELYAPPGADPDRWRSCLLRGDAPALASMLAGASIESRFRPY
                     GPGAARVACATIRPGPASRAKDWTRDSYLSDVVTARPFSSGLLVEVRLARGFRHQIRA
                     HMAWLGLPLEGDDLYGDGGNRAAELRLRAFRLSFPSPRGSGPVVVELTEPC"
BASE COUNT          309 a          616 c          629 g          282 t
ORIGIN      
        1 tcctcgccgc cgcccgtggt cgcgcactgc cagttcgggt cttcgttggc gaccttgagg
       61 atgtcctcga tgggcaccct cgcgttgaag ccgcgaatgg ccgcgagggc cttttcagcg
      121 ctggcgagct cggttgtaac cgcctcgtcg aggtcgaagc cctcccggcc aacgtcgtcg
      181 ttcagctcga agcctaggat cgcttccatc aaccgggccg agggcggctg gtccagcgag
      241 cagagtatcg ccgcgagctc cgcgagctgc tcggcggccg cgtacatcga cagctccttg
      301 agcccggaga cattggtctg aaacgccgcg agcatgcgat cgaacaggaa gctcgaaagg
      361 ctcttgagca cgtggagatt gcgcacgtcc ttgtacatca ggcggcgctt atcctcatcg
      421 atgcggccga gcgcggactc catcgccgcg aggaccgcta tgcgcacgtc ggagtcgagc
      481 atggtcgagt tctgctcggc gacgagatac gggtccgcgt cggtcgatag ctcccggtgt
      541 atctcctcga actccaggga cgcgaggaac gcgaagaacg gcgctcggtt cttctcgaga
      601 gtgcgatcga ggaggtcgta gaagtacctg gcagcctgcc tgagggtcgc gagctcccga
      661 tagaaggctt cctggagcac gcgccgcttc gcgtcgatca accccgcgct cgtcgactcg
      721 acgcgtttga ctatagcctt gaggaagcgt tccttgacga ggtcttcctt gctgccgccg
      781 ccgaagatcg accgcagggt gataatgatg cgcgtcacca agcccagctc actataggcg
      841 gcccggtaat cgatgcgcgg ctcctggagg tgtccccgct cgaacagcgg ctcggtagag
      901 accgtgcacg agcgctggag ccgctcgagc atctccgagc gctccgaggg gctgagatcg
      961 aggacgaggg cttcgaacgt cgatgcgctg gccatatccg taatcctatc cggcgaatgg
     1021 cgtattagca aggctccgtt agctcgacga cgacgggccc cgagcctcgg ggcgacggga
     1081 acgacagcct gaaggcccgg agccgtagct cggcggcccg gttcccgccg tcgccgtata
     1141 ggtcgtcgcc ctcgagcggc agtccaagcc aggccatatg cgcgcggatc tggtgcctga
     1201 agccgcgcgc gagccgtacc tcgaccagga gccccgacga gaacggccga gcggttacga
     1261 cgtcgctgag gtacgagtcg cgagtccagt ccttggcgcg agacgctggc ccgggcctga
     1321 tcgtcgcgca ggcgacgcgg gccgctcccg ggccataggg cctgaagcgg ctctcgatcg
     1381 acgcgccggc cagcatcgac gccagggccg gggcgtcgcc tcggagcagg caggagcgcc
     1441 aacggtcagg atcggctcca ggcggcgcgt acagctccgg ccgcgaaccg gcgaggccga
     1501 ccggagccgg gaggcatagc gcgcgatagg tcttgacgaa ggcgccgcgc gccgccgcgg
     1561 cgctcatcgc ggcgaagctc ccgtcgtcgg tcgcgaacgc cacgagaccc gaggtggcgc
     1621 cgtcgagccg atgcagtagc ccaccctcgc ccctaccccg gcccgccacc gcggccgcca
     1681 tgggatagcg ctcgaacagc cagtcgctga gggtagagcc agggccggcc ggagcgagcc
     1741 gcccggccgg agcgcagtgc atgccggatg gcttggcgac cacgatcacg ccgtcgcctt
     1801 cgtagaccac gcgcggctcc gctataggat cgaact
//
LOCUS       DAOOLN010000355         1833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_81222_length_1833_cov_2.960630, whole genome shotgun sequence.
ACCESSION   DAOOLN010000355 DAOOLN010000000
VERSION     DAOOLN010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81222_length_1833_cov_2.960630"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PLW80_05310"
     CDS             <1..606
                     /locus_tag="PLW80_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPB65957.1"
                     /translation="IMPDAVIMSARDFPRHWKAIVVGLRSARPKSACAVILLKGDYFP
                     FEEAAKAAYLGVNGVVKEDLSDKAELDRFQQLLKRYVEVDDARVSDRHAPGPWDRLGF
                     IFCHPVTYQPIAGAIETISSSGLSFIPETNAAVSDMEPGLVLEEASLRLDRDILSLRC
                     KLIRNGPVMAFSFEGCEPERDAIADYIAGRAEREIKALLNR"
     gene            631..927
                     /locus_tag="PLW80_05315"
     CDS             631..927
                     /locus_tag="PLW80_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPB65958.1"
                     /translation="MNVNVPCDQQRIADFARKLKAVGHPIRLRLLCLIAREQDPCVSD
                     LWQCLEQPQPVISQHLAILKQAGIVSAEVQKTKRVYSISDPFISDMIRKMVTEA"
     gene            complement(940..>1833)
                     /locus_tag="PLW80_05320"
     CDS             complement(940..>1833)
                     /locus_tag="PLW80_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65959.1"
                     /translation="YARLAAGRRPGDSGLLEDLRRERISDSHRALVEVLLLVIEYRSL
                     GELGDSIERLRECVRLLALYPEAEYWKGRLFFIEGELALAELQYERAYGMREALEVPG
                     EAYSILYAMAELYEVRSEYVAWENVMLRVIEGLDSGVDRYLEEAMLATLTEQGFNRFM
                     TLYRLDDSYSKSAHASLAAFYLERGRAAAPRHAAIAVNMILSRAIAMLKARDGDYVWT
                     DLQNFMARALGRRDVMAYLDETGFAANLFTLADALYVAGARAPALMLWGVIADQGIAP
                     LSARAGARLSDPRSAVRRGQP"
BASE COUNT          357 a          609 c          552 g          315 t
ORIGIN      
        1 atcatgccag acgccgtgat catgagcgcc cgggacttcc cgcggcactg gaaggccatc
       61 gtcgtcggac tacgctcggc gaggcccaag tcggcctgcg cggtcatcct gctcaagggc
      121 gactacttcc cgttcgagga ggcggccaag gccgcctacc tcggcgtgaa cggcgtcgtc
      181 aaggaggacc tctccgacaa ggccgagctc gaccgcttcc agcaattgct caagcgctac
      241 gtcgaggtgg acgatgccag agtctccgac cgccacgcgc ccggcccctg ggaccggctc
      301 ggcttcatct tctgtcatcc tgtaacctac cagccgatag ccggagccat cgagaccatt
      361 tcaagctcag gcctgtcgtt catacccgag acgaacgccg cggtcagcga catggaaccc
      421 ggcctcgtgc tcgaagaggc gtcactcagg ctcgataggg acatcctatc gttacgctgc
      481 aagctgatac gcaatgggcc ggtcatggcc ttctcgttcg agggatgcga gcctgagcgc
      541 gacgccatcg ccgactacat agccggaagg gccgagcgcg agataaaagc cctgttgaac
      601 cgttgaagca ctcggtgcta ttatgtggac atgaatgtca acgtaccgtg cgatcagcaa
      661 cgaatcgctg atttcgctag aaaactgaag gcagtcgggc atcccatacg gctcaggctg
      721 ctatgcctca tcgcccgaga acaggatcca tgcgtatcgg acctctggca gtgtctcgaa
      781 cagccccagc cggtcatttc ccagcacctg gccatactga agcaggccgg gatcgtcagc
      841 gccgaggtcc agaagaccaa gcgcgtctac tcgatcagcg atccgttcat cagcgacatg
      901 atcaggaaga tggtaaccga ggcctaaagg ccgacgcgat cagggctggc ctctcctgac
      961 cgccgagcgc ggatcgctta gcctcgcgcc ggcccgcgcc gatagcggcg cgatcccctg
     1021 atcggctatc accccccaca gcatcagggc cggagccctg gcgccggcga cgtacagcgc
     1081 gtcggccaag gtgaacaagt tcgccgcgaa gccagtctcg tccaagtagg ccatgacgtc
     1141 cctcctgccc agagccctcg ccatgaagtt ctgcaggtcc gtccacacat agtcgccgtc
     1201 tctggccttg agcatggcta tcgccctcga gaggatcatg ttgacggcta tggccgcgtg
     1261 cctcggggcg gcggccctcc cccgctccag gtagaacgcc gccagggagg catgagctga
     1321 cttcgagtag gagtcgtcta ggcggtacag cgtcatgaag cgattgaagc cctgctccgt
     1381 cagcgtcgcc aacatcgctt cctcgaggta ccggtccacg cctgagtcga gcccctctat
     1441 gacgcgcagc atcacgttct cccaggcgac atactccgat ctcacctcgt acagttcggc
     1501 catggcgtag agtatcgaat aggcctcgcc gggtacctcc agagcctcgc gcatcccgta
     1561 ggcgcgttcg tactggagct cggcgagggc gagctccccc tcgatgaaga acagccggcc
     1621 cttccagtac tcagcttcag gatagagggc gaggaggcgc acgcactctc gcaggcgctc
     1681 gatggagtcg ccgagctcgc ccagggatcg gtactcgatc acgagcagga gcacctcgac
     1741 gagggcgcgg tggctatcgg atatgcgttc acgtcgcagg tcctcgagga ggccgctatc
     1801 gccaggcctg cgcccggcgg cgagtcgagc gta
//
LOCUS       DAOOLN010000356         1832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_81278_length_1832_cov_3.257738, whole genome shotgun sequence.
ACCESSION   DAOOLN010000356 DAOOLN010000000
VERSION     DAOOLN010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81278_length_1832_cov_3.257738"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1832)
                     /locus_tag="PLW80_05325"
     CDS             complement(<1..>1832)
                     /locus_tag="PLW80_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPB65960.1"
                     /translation="HNGNISNTDSLQASLSARGHTVVSDNDGEIIVHLVEDHYAANKA
                     APQKALAEMRRAYAASGLSAPVADEVLLIVDAIRKAEAEADGSYAAAVADPQVPGCFA
                     IKSGSSLYAGMGKDEDGSFVVVSSDLTSVIAKTPMLIPLAEGEGLWYTEDRYLVFSLG
                     GEARFAEPRLKRSKLNVRDTGLNPRYKYYMEQEIASAPDNMDAIERYYFADDREAGLA
                     SVLEDNRSVAKELLDKACDLASAANDAALGAAFDELASSSDWRALAAKVNADAAARAP
                     LAGRTSFRSDEAQLLTDLARLRPAAAADLALLDLAMIWRKRRSVTRYARELSDSIREA
                     RKAGGTVYVVASGTSYHAALTAAYFWNGLAGVPLYPCNPGQFRSAYMDSLGNADLILA
                     VSQSGETKDLVDILQDVADTRPRIKRAALVNNENSRIPQELADFYLPILCGPEIAVAA
                     TKSFVNQLVIMYILAASYKQPEAAVKASVNRAKGLLSDTLRLSAPAIDEVAERLYLKP
                     SIHMLGTGLFGLAREAALKVREVVLNHSEGYDAAEFKHGPNTILGKNTVFSIAEVVKA
                     MDAALAAKVEGEAGKSAAELLAADPSIVEGLFSNYPLVFVCPPD"
BASE COUNT          262 a          572 c          692 g          306 t
ORIGIN      
        1 gtccggcggg cagacgaaca cgagggggta gttggagaac agcccctcga cgatggacgg
       61 atcggcggcg agcagctccg cggcgctctt gccggcttcg ccctcgacct tggccgcgag
      121 cgctgcgtcc atcgccttga cgacctccgc tatcgagaag acggtgttct tgcccaggat
      181 ggtgttcggc ccgtgcttga actcggccgc gtcgtagcct tccgagtggt tcaggaccac
      241 ctcgcgcacc ttgagggccg cctcgcgggc caggccgaag agcccggtac cgagcatgtg
      301 gatcgacggc ttcaggtaca gccgctcggc cacctcgtcg atggccggcg cggatagcct
      361 cagggtgtcg gagagcagtc ccttcgcgcg gttcacggac gccttgacgg cggcctcggg
      421 ctgcttatac gaggcggcca ggatgtacat gatcacgagc tggttgacga aactcttggt
      481 cgccgcgacg gctatctccg ggccgcagag tatcggcagg tagaagtcgg ccagctcctg
      541 ggggatgcgg ctgttctcgt tgttgacgag ggcggcccgc ttgatcctcg ggcgggtatc
      601 ggcgacgtcc tgcaggatgt cgacgaggtc cttggtctcg cctgactgcg agacggccag
      661 gatcaggtcg gcgttgccga gcgagtccat gtaggcggag cggaactgac ccgggttgca
      721 ggggtagagg ggcacgcccg cgagcccgtt ccagaagtag gcggcggtca gggcggcgtg
      781 gtaggaggtg cctgaggcta cgacgtagac ggtgccgccg gccttgcgcg cctcgcggat
      841 gctgtcggac agctcgcggg cgtatcgcgt aacggagcgg cgcttgcgcc agatcatcgc
      901 caggtcgagc agggccaggt cggcggcggc ggccggccgg agacgggcca gatcggtgag
      961 cagctgggct tcgtcggacc ggaaggaggt ccggcccgct aggggcgcgc gggccgccgc
     1021 gtcggcattg accttggccg cgagggcgcg ccagtcggac gacgaggcga gctcgtcgaa
     1081 ggccgcgccg agcgccgcgt cgttggccgc cgaggccagg tcgcaggcct tgtccaggag
     1141 ctccttggcc acggagcggt tgtcctcgag cacggaggcc aggccggcct cgcgatcgtc
     1201 ggcgaagtag tagcgctcga tcgcgtccat gttgtccggg gcggaggcta tctcctgctc
     1261 catgtagtac ttgtagcggg ggttcaggcc cgtgtcgcgc acgttgagct tggagcgctt
     1321 gagcctcggc tccgcgaagc gcgcctcgcc accgaggctg aacaccaggt agcggtcctc
     1381 ggtgtaccag aggccctcgc cctcggcgag cggtatgagc atcggggtct tggctatcac
     1441 ggaggtcagg tcgctcgaga cgacgacgaa ggagccgtcc tcgtccttgc ccatgccggc
     1501 gtacagcgac gagccggact tgatcgcgaa gcagccgggc acctgggggt cggcgacggc
     1561 ggccgcgtac gagccgtcgg cctcggcctc ggccttgcgt atcgcgtcga cgatcaggag
     1621 cacctcgtcc gcgaccggcg cgctcaggcc ggacgcggcg taggcccggc gcatctcggc
     1681 cagggccttc tgcggagcgg ccttgttggc ggcgtagtgg tcctcgacca ggtggacgat
     1741 gatctcgccg tcgttgtcag acacgaccgt atggccgcgg gcgctcaggc tggcctggag
     1801 cgagtcggtg ttcgagatgt taccgttgtg gg
//
LOCUS       DAOOLN010000357         1832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_81284_length_1832_cov_3.184018, whole genome shotgun sequence.
ACCESSION   DAOOLN010000357 DAOOLN010000000
VERSION     DAOOLN010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81284_length_1832_cov_3.184018"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PLW80_05330"
     CDS             complement(<1..561)
                     /locus_tag="PLW80_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HPB65961.1"
                     /translation="MTPLINPVTLFFASIFTSNILLTNFLGTCSFISISKDWKSSFGL
                     GMAVTVVMTITAAVSWLVLEYVIAPLELEYLSFIVFIVVIAAVVQILEMVIDRASPAP
                     YMSLGIFLPLITVNCAILGAILFMQIRRYGFVQTVAFGAGSGLGWWLAILLLAAIRER
                     TEKNPVPAGLKGPGITMITIGFMAMAF"
     gene            complement(558..1184)
                     /locus_tag="PLW80_05335"
     CDS             complement(558..1184)
                     /locus_tag="PLW80_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HPB65962.1"
                     /translation="MKAPKAVAILKDNLWANNPIFIQILGICSTLAVTNSLNNTLVMT
                     VGVALTTAFSSLTLSALKDLIPRKVRMIVQVVVISFYVILIDILLRAYLPEASRQLGP
                     YVGLIITNCIIMGRAEAFAQANPPLLSFWDGLTSGLGYMAVLMAIAAVRELLGFGTLF
                     GLRVMPEGFVPWTIMIMAPSAFFLVAMVMWWAKSVQARRQTAGKEAKK"
     gene            complement(1181..1792)
                     /locus_tag="PLW80_05340"
     CDS             complement(1181..1792)
                     /locus_tag="PLW80_05340"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPB65963.1"
                     /translation="MKKDSMLYVVLFTFAVCAVFTLALAIANEGTKDLVAANAEFATQ
                     SAVLGALGVPFDGKADAAARFAETVGAGEAPRSWLATVGGADYVAVEQSGPGLWGTIV
                     VILAATPDGERIRGVRVVSQNETPGLGGRIAEPWFLGQYEGERSPGGRVGMRAGAESS
                     GAADSDKENGRVDGVSGATRTSQSFAAIVDAALARVKESGGER"
BASE COUNT          323 a          589 c          618 g          302 t
ORIGIN      
        1 gaaggccatc gccatgaagc ctatcgttat catcgtgatg cccgggcctt tgaggcccgc
       61 cggtaccggg tttttctcgg tgcgctcgcg aatggccgcc aggagcagta tcgccagcca
      121 ccagccgagg ccggagccgg ccccgaaggc cacggtttgg acgaagccgt agcgtcggat
      181 ctgcatgaac agtatggcgc cgagtatcgc gcagttgacc gtgatcagcg gcaggaagat
      241 gcccaggctc atgtacgggg cggggctcgc gcggtcgatg accatctcga gtatctgcac
      301 cacggcggcg atgacgacga tgaacacgat gaacgacagg tactcgagct cgagcggcgc
      361 tatcacgtac tcgagcacca gccagctgac ggcggccgtg atggtcatca cgaccgtgac
      421 ggccatgccg agcccgaagg agctcttcca gtccttggag atcgagatga aggagcacgt
      481 gcccaggaag ttcgtcagga gtatgttgct cgtgaagatc gacgcgaaga acagcgtgac
      541 cgggtttatg agcggcgtca tttcttagcc tccttgccgg cggtctgtcg cctcgcctgg
      601 accgacttgg cccaccacat gaccatcgcg accaggaaga acgcgctcgg ggccatgatc
      661 atgatggtcc agggcacgaa gccctccggc atgacgcgta ggccgaacag ggtgccgaaa
      721 cccaggagct cgcggaccgc ggcgatggcc atcagcacgg ccatgtagcc gaggccgctc
      781 gtcagcccgt cccagaacga caggagcgga gggttggcct gggcgaaggc ctcggctcga
      841 cccatgatga tgcaattcgt gatgatcagc ccgacatagg ggccgagctg ccgcgaggct
      901 tccggcaggt aggcgcgtag caggatgtcg atcaggatca cgtagaacga gatgacgacg
      961 acctgcacga tcattctgac cttgcgcggt atcaggtcct tgagcgccga cagcgtcagg
     1021 ctcgagaagg cggtcgtcag cgcgacgccg acggtcatca cgagcgtgtt gttcaggctg
     1081 ttggtgaccg ctagggtcga gcagatgccg agtatctgga tgaagatggg attgttggcc
     1141 cagagattgt ccttgagtat ggcgacggct ttcggtgcct tcatcgctcg cctccgcttt
     1201 ccttgacgcg ggccagggcc gcgtcgacga tagccgcgaa cgactggctc gtgcgcgtgg
     1261 cgcccgagac gccgtcaacc cggccgttct ccttgtccga gtcggccgct cccgacgact
     1321 cggcgccggc gcgcatgccg acgcgcccgc cgggcgagcg ctctccctcg tactgcccga
     1381 ggaaccaggg ctccgcgatg cggccgccga ggccgggcgt ctcgttctgc gatacgacgc
     1441 ggacgccgcg gatccgctcg ccgtcgggcg tcgccgcgag tatcacgacg atggtgcccc
     1501 agagcccggg gccggactgc tcgaccgcta catagtcggc tccgcccacc gtggccaacc
     1561 acgaccgtgg cgcctcgccc gcgccgaccg tctccgcgaa gcgggcggcc gcgtcggcct
     1621 tgccgtcgaa cggtacgccg agggcgccga gcacggccga ctgggtcgcg aactcggcgt
     1681 tggcggcgac caggtccttg gtaccctcgt tggctatcgc gagcgcgagc gtgaagaccg
     1741 cgcagacggc gaaggtgaac agcaccacgt agagcatcga atcctttttc atggtttcgc
     1801 ctccccgccg gtagcctgag ccgccttggc gg
//
LOCUS       DAOOLN010000358         1832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_81287_length_1832_cov_3.041643, whole genome shotgun sequence.
ACCESSION   DAOOLN010000358 DAOOLN010000000
VERSION     DAOOLN010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81287_length_1832_cov_3.041643"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PLW80_05345"
     CDS             complement(<1..422)
                     /locus_tag="PLW80_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65964.1"
                     /translation="MQDRQKLVGAMFRAPGFAAVAAATVTLMYRAGLMAWPTVPLLAA
                     IKLPLLAGLVVGLALEAKAAFVISPGEAPSVGSRGVAAGAAVGDIADAEFADAAAVLA
                     SALIGYLAIARLGLNAVLASSLLGLAGALLLPKQAVAI"
     gene            complement(682..>1832)
                     /locus_tag="PLW80_05350"
     CDS             complement(682..>1832)
                     /locus_tag="PLW80_05350"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB65965.1"
                     /translation="SVRIVEAGALLDLPGEELYGYPSASAEASADGLRLAFRFGGGLL
                     TLTGGLEGGSVSGAFSQGAAGGAFSMERSAAPAAPGTARVVRGADGSALYGTLLEPTG
                     TGRPPLVILHAGLGAADRDGNNYASRGRHDALRALGEALASRGVASYRYDKRGSGESA
                     WLVPSEADQSYEAWIADLAAMVAEFGRSGRYSGVWLLGLNDGAAIAAAAANSLRAAGS
                     PVAGLVVACASADGVRDAYAKAVARAPEGLRAEGEALVASLSAGSIVDSVSSYYAEAF
                     RPSLQPYLIEAFARDLETELAAYAGPCLLVQGNMDLQATLADLLALRATRPDSLVAMP
                     LRMNHVLKDVSQDVEDNRAAFADPSYPVSAELVDAITGFIEGTFVGIP"
BASE COUNT          296 a          642 c          641 g          253 t
ORIGIN      
        1 tagatcgcga ccgcctgttt cggcagtagc agcgcgccag ctaagcccag gagcgacgag
       61 gcgagcacgg cgttgagccc cagcctcgct atcgccaggt agccgatcag ggccgacgcg
      121 agcacggcgg cggcgtcggc gaattcggcg tcggcgatat cgccgacggc cgctcccgcg
      181 gcgacgcctc gtgagccgac ggatggcgct tccccgggcg atatgacgaa ggctgccttc
      241 gcctcgagcg cgaggccgac gacgagcccc gcgaggagcg gcagcttgat ggcggccagc
      301 agcggaacgg tcggccaggc catcaagccg gcccggtaca tcagcgtgac ggtcgccgcc
      361 gcgacggccg cgaaacccgg cgcccggaac atggccccga ccaacttctg acgatcttgc
      421 atgatggatc cagtataccc gccgcccccc gatcgggcaa acgactcgcc gccatcagga
      481 tacttcgaac atgatgacag gatgaagaac agctcaagcg gataagcccc aaggcttcat
      541 cgctttatcg tgtccatcct gtctttttct tgtccatctt gttcaacgcc ttcgcctcca
      601 gggtccttcg cttcgtgatc gttgcgtctt ggtgccctta cgtctttccg cgaggcatcg
      661 cgtactcagc gtcgcgcggg attacggtat gccgacgaag gtgccttcga taaacccggt
      721 tatggcgtcg accagctcgg ctgacacggg gtacgacggg tcggcgaaag cggcacggtt
      781 gtcctcgacg tcctgggaca cgtccttgag cacgtggttc atgcgtagcg gcatagcgac
      841 gagcgagtcg ggcctggtcg ccctgagcgc caggaggtcg gcgagcgtcg cctgcaagtc
      901 catattgccc tgcactagca ggcacggccc cgcgtacgcg gcgagctccg tctccaggtc
      961 gcgcgcgaag gcctcgatca ggtagggctg gaggctcgga cggaaggctt cggcgtaata
     1021 cgaggagacg gagtcgacga tggaccccgc cgacagcgag gcgacgagcg cctcgccctc
     1081 cgcccgcagc ccctccgggg cccgggctac ggccttggcg taggcgtcgc ggacgccgtc
     1141 ggccgaggcg caggcgacga cgagtcccgc tacgggagag ccggccgccc ggagcgagtt
     1201 cgcggccgcc gccgcgatag ccgcgccgtc attgaggccc aggagccaca cgcccgagta
     1261 acggcccgac cggccgaatt ccgcgaccat cgcggccagg tcggctatcc aggcctcgta
     1321 gctctggtcg gcctcgctcg gcacgagcca ggcggactcg cccgagcccc gcttgtcgta
     1381 gcggtagctg gcgacgccgc gagacgcgag cgcttcgccg agcgcccgga gggcgtcgtg
     1441 ccggccgcgc gaggcgtagt tgttgccgtc gcggtcggcg gcgccgaggc cagcgtgcag
     1501 gatcacgagg ggcggccgcc cggttccggt cggctcgagc agggtgccgt acagggccga
     1561 tccgtcggcg ccccgaacga cgcgagccgt tcccggggcc gccggagccg cggagcgctc
     1621 catcgagaag gcgccgccgg ccgccccctg cgagaaggcg cccgataccg aaccgccctc
     1681 gagaccgccg gtcagcgtaa ggaggccgcc gccgaaacgg aacgccagcc ggaggccgtc
     1741 ggccgaggcc tcggccgacg ccgacgggta gccgtacagc tcctcgccgg gcaggtccag
     1801 gagggcgccg gcctcgacga tacgcaccga ga
//
LOCUS       DAOOLN010000359         1829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_81502_length_1829_cov_2.803269, whole genome shotgun sequence.
ACCESSION   DAOOLN010000359 DAOOLN010000000
VERSION     DAOOLN010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81502_length_1829_cov_2.803269"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..451
                     /gene="rpsH"
                     /locus_tag="PLW80_05355"
     CDS             53..451
                     /gene="rpsH"
                     /locus_tag="PLW80_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPB65966.1"
                     /translation="MSVSDPISDMLTKVRNASMARHEKVDIPTSRLKLEIVKILKTEG
                     FIKNFKKIVQENQNFIRVFLKYDEENEPILHGIEKVSKPGRRVYAGYKGLPRVHNGYG
                     TLIVSTSEGVTTGKKAAEKKVGGELICTVW"
     gene            467..1012
                     /gene="rplF"
                     /locus_tag="PLW80_05360"
     CDS             467..1012
                     /gene="rplF"
                     /locus_tag="PLW80_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPB65967.1"
                     /translation="MSRIGYLPITVPKGVKIAVQGNKVNVEGPKGKLSEELATSLVTV
                     DVKDDVINVGRADDEKQTKAFHGLYRNLIKNMVVGVSEGYKKALVINGVGFKAEVQGK
                     LLVLSLGYSSDVYVGIPEGLAVAIEGNNQKVVVSGIDKQLVGKFASEIRGLRKPEPYK
                     GKGIRYETETIRKKVGKTGVK"
     gene            1022..1387
                     /gene="rplR"
                     /locus_tag="PLW80_05365"
     CDS             1022..1387
                     /gene="rplR"
                     /locus_tag="PLW80_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPB65968.1"
                     /translation="MSTKEISEKIRRRGARKVHVRKSISGTAERPRLTVFKSNKYLYV
                     QAIDDEAGVTIAAASTLEKALEGTKPTVEGGAKLGELMGARLGEKKIGAVVFDRNGYN
                     YHGVVKAIADGTRKAGIKF"
     gene            1397..>1829
                     /gene="rpsE"
                     /locus_tag="PLW80_05370"
     CDS             1397..>1829
                     /gene="rpsE"
                     /locus_tag="PLW80_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPB65969.1"
                     /translation="MDRRDRDDRSRDIYVEKLVKLNRTAKVVKGGRRFSFSALTVVGD
                     RSGNVGYGFGKANDVTEAIRKSIDKAKRSMVKLPVKNGTIPHDVIGEYKASRVIMMPA
                     CPGSGIIAGGAVRAIMEAGGITDIQAKCVGSRSAVNVIRATF"
BASE COUNT          442 a          515 c          589 g          283 t
ORIGIN      
        1 agcgagggcc tcattccggg cgtaaccaaa tcgagctggt aggaggactg gaatgagcgt
       61 ttccgatccc atctccgata tgctgaccaa ggtccgcaac gcgagcatgg ctcgccacga
      121 gaaggtagac atcccgacct cgcggctcaa gctcgagatc gtaaagatcc tcaagaccga
      181 gggcttcatc aagaacttca agaagatcgt gcaggagaac cagaatttta tccgggtctt
      241 cctcaagtac gacgaggaga atgaaccgat tctccacggt atcgagaagg tctccaagcc
      301 cggccgaagg gtctatgccg gctacaaggg tctgccgcgg gtgcataacg gctacggaac
      361 ccttatcgtc tcgacctcag agggcgtgac caccggtaag aaggccgccg agaagaaggt
      421 cggtggcgaa ctcatctgca ccgtctggta aaagagggga accgccatgt cgagaatagg
      481 ctatctgccg ataacggtac cgaagggcgt gaagatcgcc gtacagggga acaaggtcaa
      541 cgtggaaggc ccgaagggca agctctccga agagctcgcc acgtctctcg tcaccgtgga
      601 cgtaaaggac gacgtgatca acgtcggccg cgccgacgac gagaagcaga ccaaggcatt
      661 ccacggcctg tatcgcaatc tgatcaagaa tatggtagtc ggcgtttcgg agggctataa
      721 gaaggccctc gtcatcaacg gcgtcggctt caaggccgag gtccagggca agctcctggt
      781 actcagcctc gggtattcca gcgacgtgta cgtagggatc ccggaagggc ttgcggtcgc
      841 gatcgagggc aataaccaga aggtcgttgt ctcgggcatc gacaagcagc tcgtaggcaa
      901 gttcgcgagc gagattcgcg ggctgcggaa gcccgagcca tacaagggca agggaatccg
      961 ctacgagacc gagaccatcc gcaagaaggt cggcaagacc ggcgtgaagt aagggacggc
     1021 catgagcacg aaagagatat ctgagaagat caggcggcgg ggggctcgca aggtccacgt
     1081 ccgcaagagc ataagcggaa ccgccgagcg cccgaggctg acggttttca agtcgaacaa
     1141 gtacctgtac gtccaggcta tagacgacga ggcgggcgta acgatcgccg ccgccagtac
     1201 cctggagaag gcgctcgagg gcaccaagcc gaccgtagag ggcggcgcta agctcgggga
     1261 gctgatgggc gccaggctcg gcgaaaagaa gatcggggcc gtcgtgttcg acaggaacgg
     1321 ctacaactat catggcgtag tcaaggccat cgccgacggc acccgcaagg ccgggatcaa
     1381 attctagggg gaactcgtgg ataggagaga cagggacgac aggtcccgcg atatctacgt
     1441 cgagaagctc gtcaagctga atcgtacggc caaggtcgtc aagggcggta ggcggttttc
     1501 gttctccgcg ctaaccgtgg tgggcgaccg ctccggcaac gtcggctacg gattcggcaa
     1561 ggctaacgac gtgaccgagg cgatccgcaa gagcatcgac aaggccaaga ggagcatggt
     1621 caagcttccc gtgaagaacg gcaccatacc tcacgacgtc atcggcgagt acaaagcctc
     1681 gagggtcatc atgatgcccg cctgccccgg ttccggcatc atcgccggcg gcgcggtccg
     1741 cgcgatcatg gaggccggcg gtatcaccga catccaggcc aagtgcgtcg ggagccgctc
     1801 cgccgtcaac gtgatccgcg ccaccttca
//
LOCUS       DAOOLN010000360         1823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_81909_length_1823_cov_3.679299, whole genome shotgun sequence.
ACCESSION   DAOOLN010000360 DAOOLN010000000
VERSION     DAOOLN010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81909_length_1823_cov_3.679299"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="PLW80_05375"
     CDS             <1..1076
                     /locus_tag="PLW80_05375"
                     /inference="COORDINATES: protein motif:HMM:NF024586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPB65970.1"
                     /translation="FYPDKAAKGSLYLVDGGMIAGVKKYAPGLAAHCVSYSGLGQIER
                     ALDGLEAYDGRSPIFLELLACEGGCVNGPEAQHRAGSVRKRLAVLDYARSSADRRDAQ
                     SAPVAERVAEPVANESPSEESVSEALRSIGKTRPEHELNCSSCGYDSCRAFAAAMISG
                     KAERTMCVSYMRQLAQKKANALIKAMPSGVVLVDTDLRVVECNRPFARLLGPEVEAMF
                     DAKPGLEGADLRRLVPFWQLFEAAFADDGLSEPANGNRRESGPRDVKFDGRVLSGSVF
                     AVEPGLVVGGIFQDVTVPWIRRDRVVSQAKAVIRKNVATVQKIAYLLGENAAESETIL
                     QSIIESFDAGDHGPESRDRQKRG"
     gene            1083..>1823
                     /locus_tag="PLW80_05380"
     CDS             1083..>1823
                     /locus_tag="PLW80_05380"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HPB65971.1"
                     /translation="MKDSFLELSHHQVSKRGQSAPGDAFQSIKCDDGRVIAVLSDGLG
                     SGIKAGVLATLTATMAARCVAADIPLRRVAEFVMRSLPVCSERRISYATFTIVDVAPG
                     GSVRVVEYDNPPYLLLRRGQALLPEKTELRLPRGRGGAKGDAVLKLSSFDAEPGDRVV
                     LYSDGVTQAGMGTRSYPLGWGDEAVKRFATAAVAEAPDGVSARELARTIVHEATAKDA
                     WAPKDDITCGVAYWRHPRRLLVLTGPPVS"
BASE COUNT          288 a          650 c          615 g          270 t
ORIGIN      
        1 gtttctaccc cgacaaggcg gcgaagggct cgctctacct ggtggacggc ggcatgatag
       61 ccggcgtcaa gaagtacgcg cccggcttgg cggcccactg cgtctcgtac tccggcctcg
      121 ggcagatcga gcgcgccctg gacggcctcg aggcctacga cggacgctcg cccatcttcc
      181 tcgagctgct cgcctgcgaa ggcggctgcg tcaacgggcc cgaggcccag caccgcgccg
      241 gctcggtacg caagcgcctc gcggtactcg actacgcccg ctcctcggcc gatcgccgcg
      301 acgcgcaatc tgcccccgtc gccgagaggg tggccgagcc tgtagccaat gaaagcccct
      361 ccgaggagtc cgtgtccgag gcgctccgct cgataggcaa gacccggccc gagcacgaac
      421 tcaactgctc gtcctgcggc tacgacagct gccgggcctt cgccgccgcg atgatctccg
      481 gcaaggccga gcgcacgatg tgcgtgtcgt acatgcgcca gcttgcgcag aagaaggcca
      541 acgcgctgat caaggccatg ccgtccgggg tcgtgctcgt cgataccgac ctccgggtcg
      601 tcgagtgcaa ccggcccttc gccaggctac tcgggcccga ggtcgaggcc atgttcgacg
      661 ccaagccggg gctcgagggc gccgacctcc gcaggctcgt gccgttctgg cagctcttcg
      721 aggcggcctt cgccgacgac gggctatcgg agccggccaa cggcaaccgt cgcgaatcgg
      781 gtcctcgcga cgtcaagttc gacggccgag tgctgtcggg ctcggtcttc gcggtcgagc
      841 cagggctcgt cgtcggcggc atcttccagg acgtgaccgt gccgtggata cgcagggacc
      901 gcgtcgtctc ccaggccaag gccgtgatac gcaagaacgt ggccaccgtg cagaagatag
      961 cctacctgct cggggagaac gccgccgaga gcgagaccat actccagtcg atcatcgagt
     1021 cgttcgacgc cggcgaccac ggccccgaga gccgcgaccg acagaagcgc ggctagccgg
     1081 ccatgaagga ctccttcctc gagctatcgc atcaccaggt atccaagcgc gggcagagcg
     1141 cgccgggcga cgcgttccag tcgatcaagt gcgacgacgg gcgcgtcata gcggtgctgt
     1201 ccgacggcct gggctccggc atcaaggccg gcgtgctggc cacgcttacg gcgacgatgg
     1261 ccgcgcgttg cgtcgccgcc gacatcccgc tccgccgcgt cgccgagttc gtcatgcgct
     1321 cgctaccggt ctgctcggag cgtagaatca gctacgcgac cttcaccatc gtcgatgtgg
     1381 cccccggcgg ctcggtgcgc gtcgtggagt acgataatcc gccgtacctg ctcctgcgtc
     1441 gcggccaggc tctcctcccg gaaaagacgg agctcaggct acctcgcggc cgcggcggag
     1501 ccaagggcga cgcggtgctc aagctgtcca gcttcgacgc cgagccggga gaccgcgtcg
     1561 tgctgtactc cgacggcgtc acgcaggcgg gcatgggtac gaggagttac cccctgggct
     1621 ggggcgacga ggcggtcaag cgcttcgcta cggcggccgt ggcggaagcc cccgacggcg
     1681 tctccgcgag ggagctcgcc aggactatcg tgcacgaagc caccgccaag gacgcctggg
     1741 ccccgaagga tgacataacc tgcggcgtgg cgtactggcg ccacccgagg cgcctcctcg
     1801 tgctgaccgg cccgccggtc tcc
//
LOCUS       DAOOLN010000361         1823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_81929_length_1823_cov_2.677036, whole genome shotgun sequence.
ACCESSION   DAOOLN010000361 DAOOLN010000000
VERSION     DAOOLN010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81929_length_1823_cov_2.677036"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..688)
                     /locus_tag="PLW80_05385"
     CDS             complement(212..688)
                     /locus_tag="PLW80_05385"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPB65972.1"
                     /translation="MPANGIIHLSDASLIAIHALVGLADAEGRLVQGRDLAAIIDASE
                     NHLAKVMQRLVRASIVRSVKGPNGGFALDKRPEDISFKEAIEAVDGPISSGFCPFRTD
                     RCNPNNCIFGKEISKHAFELVAYLSQRTVADIARERAPDSPPAYHAVIGNRTKRGA"
     gene            872..1312
                     /gene="aroQ"
                     /locus_tag="PLW80_05390"
     CDS             872..1312
                     /gene="aroQ"
                     /locus_tag="PLW80_05390"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HPB65973.1"
                     /translation="MLISVINGPNLNMLGEREPQVYGSETLDDLNSALGRAARDRGVD
                     VEFFQSNSEGSLIDRLHECSRRSDGVILNAGAYTHYSIAIRDAIAAIRPPVVEVHLSN
                     VHAREEFRRVSVIAPACVGQISGFGSTSYLLALEALVRIYEERS"
     gene            1309..1620
                     /locus_tag="PLW80_05395"
     CDS             1309..1620
                     /locus_tag="PLW80_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65974.1"
                     /translation="MSVFVSVEDPEGEPLAGVFELERLFGRFASVPGYCLRFVGEGDD
                     ASFNAVQTPLLLAELESIDATAFGAAERAELVRLVALCRKYAGKERTHIRFYGEASSS
                     E"
     gene            1617..>1823
                     /locus_tag="PLW80_05400"
     CDS             1617..>1823
                     /locus_tag="PLW80_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65975.1"
                     /translation="MSEPNDTMDTVASAIAEASPSETTRRFAFRGTEALVRASAFDGY
                     PAPDRGTMAGASPWEAPGDELLAVA"
BASE COUNT          328 a          553 c          565 g          377 t
ORIGIN      
        1 catagtccgc agccatcgca cttatcctcg tctatctcta tgaccgttcg tgtagccatg
       61 atctcctcct cgtctcgatg ttgacttatg tcgtgcttgc attcataagg gtaaagagga
      121 actataaaac cgaaaaaccg atatttaaat cctctcctac catactatct gttattgtcc
      181 taactcgccg tcagccgcgg tcagccatga ttcacgcccc gcgtttagtc ctattgccga
      241 tgaccgcatg ataggccggc ggcgagtcgg gcgcgcgttc gcgcgcgatg tccgccaccg
      301 tccgctggct caggtaggcc acgagctcga aggcgtgttt ggatatttct ttgccgaaga
      361 tacagttgtt cggattgcaa cggtcggttc ggaacgggca gaacccgctc gagataggac
      421 cgtcgacggc ctcgatcgcc tccttgaacg agatatcctc ggggcgctta tcaagagcga
      481 agccgccgtt cgggcccttg accgagcgca cgatactggc gcggacgagt cgttgcatta
      541 ctttggcaag gtggttctcg ctcgcgtcga tgatggccgc gaggtcgcgg ccttggacca
      601 gccgaccctc ggcgtcggcg aggccgacga gcgcgtgaat cgcgatgagg ctggcgtctg
      661 aaagatggat gatcccgttc gcaggcattg tggtatttta ataccacaat taatatccac
      721 tgtcaagcgc ttgtatcccg attcggtcga actgtatcgg ctagcccacc acaaggaggt
      781 gcctgcggtc gcgacgctcc agtccatagt ttgacagcag cgtctcgcgc gtatagagtt
      841 gtcccgcggt ggaggccgga gcgtgaaaac gatgctgatt tccgtcatca acgggcctaa
      901 tttgaacatg ctcggcgagc gcgaaccaca ggtgtacggt tccgagacgc tcgacgacct
      961 caatagcgcc ttgggccggg cggcccgcga tcgcggtgtc gacgtcgaat ttttccagtc
     1021 caattccgag ggctcgctta tagaccgcct gcatgaatgc tcccggcgct ccgacggggt
     1081 cattctcaac gccggcgcgt acacccacta ctcgatagcc atccgagacg ctatcgccgc
     1141 catcaggccc ccggtcgtcg aggtacacct ctccaatgtc cacgcccgcg aggaattcag
     1201 gcgggtttcg gtcatagcgc cggcatgcgt cggccagata tccggcttcg gttcgacgag
     1261 ctacctgctg gcgctcgagg cgctggtccg tatatacgag gagcgatcat gagcgtattc
     1321 gtatcggtcg aggatccgga gggagagccg ctcgctggag tcttcgagct tgagcgtctg
     1381 ttcgggcgtt tcgcgtcggt acccggctac tgcctccgct tcgtgggtga gggcgacgac
     1441 gcatcgttca acgcggtcca gaccccgttg ctgttggcgg agctcgagtc catcgacgcc
     1501 accgcgttcg gcgcggcgga gcgggccgag ctggtgcgtc tcgtcgcgct gtgccgtaaa
     1561 tacgcgggca aggagcgcac gcatatccgc ttctacggcg aggcgtcgtc atcggaatga
     1621 gcgagccgaa cgatacgatg gatacggtag cttcggccat cgccgaagcc agcccgagcg
     1681 agaccacgcg acgcttcgcg ttccgaggca ccgaggcgtt ggtccgggcc tccgcgttcg
     1741 acggctaccc ggcgcccgat agagggacga tggccggcgc gtcgccgtgg gaggcgccag
     1801 gggacgagct tctcgccgtg gcg
//
LOCUS       DAOOLN010000362         1818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_82284_length_1818_cov_2.959161, whole genome shotgun sequence.
ACCESSION   DAOOLN010000362 DAOOLN010000000
VERSION     DAOOLN010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82284_length_1818_cov_2.959161"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PLW80_05405"
     CDS             complement(<1..286)
                     /locus_tag="PLW80_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HPB65976.1"
                     /translation="MTLVNTDYVSGKELETLGLVKGSTIQSKNIGKDILQAFKSMVGG
                     ELRAYNDMMNEARALATKRMVEEAESLGADAVVNIRYASSSIMQNAAEVIA"
     gene            complement(267..494)
                     /locus_tag="PLW80_05410"
     CDS             complement(267..494)
                     /locus_tag="PLW80_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65977.1"
                     /translation="MNEHGRKMVAPAVIVICLVAYYAAVALLILKLELPRLASVGIVV
                     FSALLSGILIWTLVDRIKEIRKGEEDDLSKY"
     gene            complement(695..>1818)
                     /locus_tag="PLW80_05415"
     CDS             complement(695..>1818)
                     /locus_tag="PLW80_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HPB65978.1"
                     /translation="ALMKGCDDAKTCVVQIAPAVRVALGEEFGLEPGSLVTKKVYTAL
                     RRLGFDKVFDTNFSADLTIMEEGTEFVKRFTDYAAGKDKGMLPLITSCCPAWVDYMEK
                     YYDDMIPNFSTAKSPQQMMGAMIKTFWAEQAKVDPKKIYSVSIMPCTAKKWECGRNDD
                     MWSSGEKDVDVVLTTRELARMIKQAGIDLVNLPEEEADSPLGPYTGAGTIFGTTGGVM
                     EAALRSAYYLVTKEDLKDVNFTGVRGISGIKEARIHIKGIEVRVAVAHQMGNIAAVLE
                     EVRKAKAEGKDVPWHFIEVMACRGGCIGGGGQPYGATDEVRQKRMAGLYTDDEKSTLR
                     CSHHNPAIQYAYKEFLGEPNGHKAHELLHTSYKKLELYK"
BASE COUNT          323 a          597 c          525 g          373 t
ORIGIN      
        1 acgcgatgac ctcggcggcg ttctgcatga tagaggagga cgcgtagcga atattcacca
       61 ccgcgtcggc gccgagcgac tcggcctcct cgaccatccg cttcgtcgcc agcgccctgg
      121 cctcgttcat catatcgttg tacgcccgca gctccccgcc gaccatcgac ttgaaggcct
      181 gcaggatatc cttgccgatg ttcttcgact ggatggtgct gcccttgacg agccccagcg
      241 tctcgagctc cttgccggaa acgtaatcag tatttactaa ggtcatcctc ttcccccttc
      301 cggatctcct tgatcctgtc gaccagcgtc cagatcagta tacccgatag gagagcggaa
      361 aatactacga taccgacgct cgcgagcctc gggagctcca gcttgagtat caatagcgcc
      421 accgccgcat aatacgccac aagacagatc acgatcaccg ccggcgcaac catcttccgt
      481 ccatgctcgt tcatatccgg cctccgtcgc ctatactata cacaaacccg cccggtacga
      541 tacgccgcag gaccgcgcca gcgatccgag ccggcgtatc gttcctccta caataagaac
      601 gcccgggccg cgaggcccgg gcgatacgaa ccgcaccagg acgttaagcc gtaaaaaccg
      661 catagcggtt tatattggct tcaacgtccg gcaattactt gtacagttcc agcttcttgt
      721 agctcgtgtg caggagctcg tgcgccttgt gcccgttggg ctcgccgagg aactccttgt
      781 acgcgtactg gatagccggg ttgtggtgcg agcagcgcag cgtgctcttc tcgtcgtcgg
      841 tgtacaggcc agccatgcgc ttctgcctga cctcgtcggt cgcgccgtag ggctgaccgc
      901 cgccgccgat gcagccgccg cggcaggcca tcacctcgat gaagtgccat ggaacatcct
      961 tgccctcggc cttggccttg cggacctcct cgagcaccgc ggcgatgttg cccatctggt
     1021 gggccaccgc cacgcggacc tcgatgccct tgatgtggat gcgagcttcc ttgatgccgg
     1081 agatgccgcg gacgccggtg aagttgacgt ccttgaggtc ttccttggtg acgaggtagt
     1141 aggcggaacg gagcgccgcc tccatgacgc cgccggtcgt accgaagatc gtgccggcgc
     1201 cggtatacgg accgagcggg ctgtcggcct cttcctcggg aagattcacg aggtcgatgc
     1261 cagcctgctt gatcatgcgg gccagctcgc gggtcgtcag cacgacgtcc acgtccttct
     1321 cgccggagct ccacatgtcg tcgttgcggc cgcactccca cttcttggcg gtacagggca
     1381 tgatcgatac cgagtagatc ttcttcggat caaccttggc ctgctcggcc cagaaggtct
     1441 tgatcatcgc gcccatcatc tgctgcgggc tcttcgcggt ggagaaattc ggaatcatgt
     1501 cgtcgtagta cttctccatg taatccaccc aggccgggca gcaggaggtg atcagcggca
     1561 acatgccctt gtccttgcca gcggcgtagt cggtgaagcg cttaacgaac tcggtgcctt
     1621 cctccatgat ggtcaggtcg gccgagaagt tcgtgtcgaa caccttgtcg aagccgaggc
     1681 ggcgcagggc ggtatagacc ttcttggtga ccagggatcc aggctcgagg ccgaactcct
     1741 cgccgagcgc cacacggacc gcgggggcta tctggaccac gcaggtcttg gcgtcgtcgc
     1801 accccttcat cagcgcgt
//
LOCUS       DAOOLN010000363         1816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_82448_length_1816_cov_2.425327, whole genome shotgun sequence.
ACCESSION   DAOOLN010000363 DAOOLN010000000
VERSION     DAOOLN010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82448_length_1816_cov_2.425327"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PLW80_05420"
     CDS             <1..620
                     /locus_tag="PLW80_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB65979.1"
                     /translation="RSAAIVIREQGPRSATLKNISGRAGVTEPAIFRHFDGVDGMFES
                     LHAVAALFYARFTACLEGDEPTGMDRLEAGVSRIFDSLAANADFAYVVAKPDPIFRQY
                     PRLKEKDAGMYASLAEAFTDCFKSAKADGSLLPGADPSALASAALGLFYQVLYAWVDD
                     VEGYSLHRDGMKAFRAYLAIARNPGRAASSGAKAPAKTKGAAKKR"
     gene            665..1147
                     /locus_tag="PLW80_05425"
     CDS             665..1147
                     /locus_tag="PLW80_05425"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HPB65980.1"
                     /translation="MRVLLTGGVKSGKSSRALELARDFAPDRWFLATATPFDDEMRER
                     IRRHRAERATDFVTVEEPIDIDAAVRERMVVDCVPMWLNNLFVADREDDWEPILDRFI
                     ARLPRDIVIVTNEIGMGVIPADPTSRRYGMALGRANAKLAAAVDSVELLVAGIPMKVK
                     "
     gene            1144..>1816
                     /gene="cbiR"
                     /locus_tag="PLW80_05430"
     CDS             1144..>1816
                     /gene="cbiR"
                     /locus_tag="PLW80_05430"
                     /inference="COORDINATES: protein motif:HMM:NF041277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobamide remodeling phosphodiesterase CbiR"
                     /protein_id="HPB65981.1"
                     /translation="MSSRVSVPSYVIPGSYVENLRFLYERTSQRQVELLFFIYDDDAR
                     AMLRSEAREIESYSADFGYTVHMPDAIEPGHEELLERTADYASAFIVHPPRADDGLRA
                     FVALLDEWRGRYGPDRFLLENTTLARFDAADTAAADSNYGPPRVCADVGHLRAEGRDP
                     AAWIAERADRVGELHVHGFDGSRDHVGFAADEPWILELAPFARGFDGIVELELFSWAE
                     AEAGSA"
BASE COUNT          299 a          633 c          597 g          287 t
ORIGIN      
        1 tccgttcggc ggccatcgtt atacgtgaac agggcccccg ttcggctacc ctcaagaata
       61 tctccggtag agccggggtg accgaacccg ccatattcag gcacttcgac ggcgtcgacg
      121 gcatgttcga gtcgttgcac gccgtggcgg cgctattcta cgcgcgattc acggcctgcc
      181 tcgagggcga cgagccgacg ggcatggaca ggctcgaggc cggcgtcagc cgtatattcg
      241 actcgctggc cgccaacgcc gacttcgcgt acgtcgtcgc caagcccgac ccgatcttca
      301 ggcagtaccc gaggctcaag gagaaggacg ccgggatgta cgcttccctg gctgaagcct
      361 tcaccgactg cttcaagtcc gccaaggccg atggcagcct cctgcccgga gcggacccct
      421 cggcgctggc ctcggcggcc ctgggcctct tctaccaggt actctacgcc tgggtcgacg
      481 acgtcgaggg ctacagcctc catagagacg gcatgaaagc ctttcgggcc tacctggcta
      541 tcgccaggaa ccctggccgg gccgcctcgt ccggcgccaa ggctccagcc aagaccaagg
      601 gcgctgccaa gaagcgctga cccgtcggct tgacgcgccc gggagcggcc gttactgtcg
      661 agtcatgcga gtactactga ccggcggcgt gaagtcgggc aagtcgagcc gagcgctcga
      721 gctagccagg gacttcgcgc cggaccgatg gttcctggcg acggctacgc ccttcgacga
      781 cgagatgcgt gaacgcatcc gcaggcacag ggcggagcgg gccacggatt tcgtcaccgt
      841 cgaggagcct atagacatag acgccgcggt gcgcgagcgc atggtcgtcg actgcgtacc
      901 gatgtggctc aacaacctct tcgtcgccga cagggaggac gactgggagc ccatcctgga
      961 ccggttcatc gcgcgcctgc cgcgcgatat cgtcatcgtc accaacgaga taggcatggg
     1021 cgtgatcccc gccgacccga cgagccgccg ctacggcatg gcgctcgggc gcgccaacgc
     1081 caagctggcc gccgccgtcg actcggtgga gctcctcgtc gcggggatcc cgatgaaggt
     1141 caaatgagct cccgcgtctc cgtgccgtcc tacgtgatcc ccgggagcta cgtcgagaac
     1201 ctacgtttcc tctatgagcg cacgagccag cggcaggtag agctgctgtt cttcatctac
     1261 gacgacgatg cgcgcgccat gctacgtagc gaggcccggg agatcgagtc gtactcggca
     1321 gacttcggct acacggtgca tatgcccgac gccatcgagc ccgggcacga ggagctgctc
     1381 gaacgcacgg cggactacgc gtcggcgttc atcgtgcacc ccccgcgcgc cgacgacggc
     1441 ctccgggcct tcgtcgccct gctcgacgag tggcggggac gctacggacc ggatcgcttc
     1501 ctgctcgaga acacgacgct ggccaggttc gacgcggctg acaccgccgc cgccgactcg
     1561 aactacggcc ctccgcgcgt ctgcgccgac gtggggcacc tacgcgccga ggggcgcgac
     1621 ccggccgcct ggatcgcgga gcgggccgat cgcgtcggcg agctccatgt ccacggcttc
     1681 gacggctcgc gcgaccacgt cggcttcgcc gccgacgagc cgtggatact cgagctagcc
     1741 ccgttcgcgc gcggcttcga cggcattgtc gagctggagc tattctcgtg ggccgaggcc
     1801 gaggcgggat cggccg
//
LOCUS       DAOOLN010000364         1814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_82557_length_1814_cov_2.594656, whole genome shotgun sequence.
ACCESSION   DAOOLN010000364 DAOOLN010000000
VERSION     DAOOLN010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82557_length_1814_cov_2.594656"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PLW80_05435"
     CDS             complement(<1..679)
                     /locus_tag="PLW80_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPB65982.1"
                     /translation="MATTKKTAILLATLTAFIWGLSFLSIKTAVAVIPPITLGLARFI
                     VADVVLLAAFAIMRKRPRLAPKDLPLMAGAGLVGVTLYFLGENNGVLLLSASEASIIV
                     GTIPVITMLAERLFTKALLSPWQYAGAAASALGVALIVVESLRLSPAPLGYLYMALAA
                     LSWVAYAFMTKPLLGTYEPLEVTFWQSVFGAAGFVPFALVVRTVWSAVTPVVALNVLY
                     LGIFCSAV"
     gene            complement(696..1661)
                     /locus_tag="PLW80_05440"
     CDS             complement(696..1661)
                     /locus_tag="PLW80_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65983.1"
                     /translation="MTRRSKDTIRKAAIVAAGVAMIASLAILTSARIQSRFVEGQSGS
                     ALVFIDKYVDEPLRAEFAAGIDSYTGGFLKAAGLLAEAPIRGPVAFFAADRHYGVISA
                     NDPLVKRRLSGRPSSDFVLFRSIGSNFYANELVARSIASQDGAYVFVNIDEGWRASLV
                     HAYAHSIAAANAPAALSPAFVLDQDFDSEMKFSYRFADETFALLASDLLELAEDLGSV
                     EQAWSSFSAATARRYADPESDVAAREAEIIMSTYDLPQKSAEFYAACNGFAAWLLDAY
                     GRDALCAAARTFFSGRYDGLDDPFIAFGGLEKAVLEWNGDATIPR"
BASE COUNT          324 a          582 c          623 g          285 t
ORIGIN      
        1 cgaccgccga gcagaatata cccaggtaca gcacgttgag cgccacgacc ggggtgacgg
       61 ccgaccagac ggtcctcacg acgagggcga acgggacgaa ccccgcggcc ccgaacacgc
      121 tttgccagaa cgtcacctcg aggggctcgt acgtgccgag caggggcttc gtcatgaagg
      181 cgtaggccac ccaggacagg gccgccaggg ccatgtacag gtagccgagc ggggccgggc
      241 tcaggcgtag cgactcgacc acgatcaggg cgacgcccag ggccgatgcc gccgctccgg
      301 cgtactgcca aggcgacagg agcgccttgg tgaacagccg ctcggcgagc atcgttatca
      361 ccggtatggt gccgacgatg atcgacgcct cggaggccga caggagcagt acgccgttgt
      421 tctccccgag gaagtacagc gtcacgccga ccaatccggc gccggccatc agtggcaggt
      481 ctttcggcgc gagcctgggc cgcttgcgca tgatggcgaa ggcggcgagt agcacgacgt
      541 cggcgacgat gaagcgggcc aagccgagcg tgatgggcgg gatcactgcc accgccgtct
      601 tgatgctaag gaacgaaagg ccccagatga aggccgtaag cgtggcgagc agtatcgccg
      661 tcttcttggt tgtcgccatg gtacgtcgcg cgatcctacc gggggatcgt cgcgtccccg
      721 ttccattcca gtaccgcctt ctccaggccc ccgaaggcta tgaacggatc gtcgaggccg
      781 tcgtagcgcc cggagaagaa ggtcctggcg gcggcgcata gcgcgtcgcg gccgtaggcg
      841 tcgaggagcc acgcggcgaa gccgttgcac gcggcgtaga attccgccga tttctgcggt
      901 aggtcatagg tggacatgat gatctcggcc tcgcgggccg ccacgtcgga ttcggggtcg
      961 gcgtagcgcc tcgccgtggc cgccgagaac gacgaccagg cctgctcgac gctccccagg
     1021 tcctcggcca gctcgaggag gtcgctcgcg agcagggcga aggtctcgtc ggcgaaacgg
     1081 tacgagaatt tcatctcgga atcgaagtcc tgatccagga cgaatgccgg cgacagggcg
     1141 gccggcgcat tggcagcggc tatcgagtgg gcgtaggcgt ggacgagcga ggcccgccag
     1201 ccttcgtcga tattgacgaa gacgtaggcg ccgtcctggg acgctatcga cctcgccacc
     1261 agctcgttgg cgtagaaatt ggatcctatc gacctgaaga gcacgaagtc cgacgacggc
     1321 cgcccgctga ggcgccgctt gacgagcggg tcgttggcgc tgatgacgcc gtagtgccga
     1381 tcggcggcga agaaggccac cggcccgcgt atcggggctt cggcgagcag gccggcggct
     1441 ttcaggaagc ctccggtata ggagtcgatg cccgccgcga attctgcgcg cagaggctcg
     1501 tcgacgtact tgtcgatgaa tacaagcgcg cttccggact ggccctccac gaagcggctc
     1561 tgtatccgcg ccgaggtcag gatggcaagc gaggcgatca tggcgacgcc tgccgccacg
     1621 atcgccgcct tccgtatcgt atccttgctc cgtctcgtca tgttcgaacc tctcgggcga
     1681 tactatcccc agcgccgtgg gccgcgcaag cggcgtcgag cttcgacggc gtcggcccgg
     1741 cgtatttgac cattccgtcc attcgtcgca taatacgcgg cgtcgggccg cgagaagccg
     1801 cgcttcaccc tcgg
//
LOCUS       DAOOLN010000365         1811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_82798_length_1811_cov_2.829157, whole genome shotgun sequence.
ACCESSION   DAOOLN010000365 DAOOLN010000000
VERSION     DAOOLN010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82798_length_1811_cov_2.829157"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1696)
                     /locus_tag="PLW80_05445"
     CDS             complement(<1..1696)
                     /locus_tag="PLW80_05445"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HPB65984.1"
                     /translation="MVRTTNYPAEAARVRSPRSGARTGARRGLLLAAAALCAASSSCS
                     LFMSIFYGELRTIGVSASALGSVTVEADAPNESRVDGRYAFRDGTRVTLRAEPSEGGV
                     FDSWSGGYTSYDAEFSFTVAKNAELTANFVSLHDPWLVMIYMAAANTLEADGYYDLNR
                     MELGLAEAAPALRGNLKVVALFDRISTADYLAANPGVNTSYNSSDDGDWSGARLFEVR
                     PDRDPASLASHVIPSASSSLGVWRTGSDDEMDMGDRGNLTAFAAWATEAYPEYRRNAL
                     ILWNHGSGLAASRSIGFDDEVYVDDGRYGSAGQLYIGELDEALEPVYGPGGLAGHRLE
                     AFGADACHLGLYEAAYELRELAGYFAASPATEYGGWNYARLFSTEAAFDSGRAFVREV
                     VQGYEAKSTFYPNTMTALDLAGMESLKTAVDGFGAALLDYRDGGADEAERSVRQAAIE
                     ALRNASVAYYSGSQYDLPLYAPYHDLGDFSERVNKAALPADATAAAEAVLEALSSAVV
                     YTWTASEYGGFKGACGRGLGIFFSHGDETHTLNGQARSHYAYQGYYTDGYSGIQDIP"
BASE COUNT          284 a          590 c          632 g          305 t
ORIGIN      
        1 agggaatatc ctggataccg ctgtagccgt cggtgtagta gccctggtag gcgtagtggc
       61 tgcgcgcctg tccgttcagc gtatgggtct cgtcgccgtg gctgaagaag atgcccagtc
      121 cgcggccgca agcgcccttg aagccgccgt actcgctcgc ggtccaggtg tagacgacgg
      181 ccgaggacag ggcctccagc actgcctcgg cggcggcggt ggcgtcagct ggtaaggcgg
      241 ccttattcac gcgttccgag aagtcgccga ggtcgtggta gggggcgtag agcggcaggt
      301 cgtactgaga gccggaataa taggcgacgc tcgcgttacg gagcgcttcg atcgcggctt
      361 ggcgaacgga tcgctccgct tcgtcggcgc caccgtcgcg atagtcgagc agggccgctc
      421 cgaacccgtc gaccgccgtc ttgagcgact ccatgcccgc taggtccagc gccgtcatcg
      481 tgttcggata gaacgtcgac ttggcctcgt agccctggac gacctccctg acgaaggcgc
      541 ggccggaatc gaaggcggct tcggtggaga agaggcgggc gtagttccag cctccgtact
      601 cggtggccgg cgaggccgcg aaatagccgg ccagctcgcg tagctcgtag gccgcctcgt
      661 agaggcccag gtggcaggcg tcggcgccga acgcctcgag ccggtggccg gccaggcctc
      721 ccgggccgta gaccggctcg agcgcctcgt caagctcgcc tatgtatagt tggcccgccg
      781 agccgtagcg gccgtcgtca acgtaaacct cgtcgtcgaa accgatcgag cgagacgccg
      841 cgagcccgct gccgtggttc catagtatca gcgcgttccg cctatactcg gggtaggctt
      901 ccgtcgccca ggccgcgaag gccgtcaggt tgccgcggtc gcccatatcc atctcgtcgt
      961 cgcttccggt ccgccagact ccgaggctcg acgaggcgct cgggatcacg tgggaggcca
     1021 ggctggccgg gtccctgtcc ggcctgacct cgaagagcct ggcgcccgac cagtcgccgt
     1081 cgtcggacga gttgtaggag gtattgactc ccggattggc ggccaggtag tcggcggtgg
     1141 agatgcggtc gaacagcgcg accaccttga ggttgccgcg cagggccggc gcggcctcgg
     1201 ccagtcccag ttccatacgg ttcaggtcgt agtagccgtc cgcctcgagg gtgttggcgg
     1261 cggccatgta aatcatcacg agccatggat cgtggagcga gacgaaattc gccgtaagct
     1321 cggcgttctt ggccacggta aagctgaact cggcgtcgta gctcgtgtag ccgccgctcc
     1381 acgagtcgaa gacgccgcct tcagagggct cggctcgtag cgtcacgcgg gtgccgtcac
     1441 ggaacgcgta ccgtccgtcg acgcgcgact cgttcggggc gtcggcctcc accgtcaccg
     1501 aaccgagggc tgaggcggag acgccgatgg tccgtagctc gccgtagaag atgctcatga
     1561 acagcgagca cgacgacgag gccgcgcata gcgcggccgc cgcgagcagg aggccgcgcc
     1621 gcgcgccggt tcgcgcgcca gagcgcgggc tccgtacccg agcggcctcg gccgggtagt
     1681 tcgtcgtcct taccattgta accaaactat agcacgcggc tcggtctctt acagcgcctt
     1741 ggcccgcacg cgctccatgg cccagagcac gtacttgcgt acgcgggcgt cggggtcgtt
     1801 ctggagctcg g
//
LOCUS       DAOOLN010000366         1811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_82799_length_1811_cov_2.818337, whole genome shotgun sequence.
ACCESSION   DAOOLN010000366 DAOOLN010000000
VERSION     DAOOLN010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82799_length_1811_cov_2.818337"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLW80_05450"
     CDS             complement(<1..211)
                     /locus_tag="PLW80_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65985.1"
                     /translation="MRISTATRSAAALAVILLASCAGLGSRPGPAEPPSLPGAAVDTT
                     VPAELSAEEKERMALDALGRSMDSGD"
     gene            355..>1811
                     /locus_tag="PLW80_05455"
     CDS             355..>1811
                     /locus_tag="PLW80_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65986.1"
                     /translation="MRISAFGRAAAMAFVYIAVFVVLVMVQFPSGGPITEVSGGVSFK
                     GLPDGDGVRSAELVANGLRLVFSERYPLLLSGGAGPDGALYPVAYEAVDDGFIVRFDD
                     GTRLFVNADDEGRASWRLDAKRSKKASATMRYELAYGAALLAPGDDGSIRLSFGDATY
                     RVSGVTTGSEPRTLSLKATGGAFRAFASIRETKDAAETPAQFLAQAPMDPALWSREIG
                     AWRDKAWSSVSGADFDAASGTWSGAFDEPSFVLYLAEAMRRDLRDAAAALVAVARSSH
                     ADSLSWKSAPFAGKTATSMAAFEAANLAEVKATERLVQARAGTMFYRRGIVPLLFDRA
                     PYSLAQEAMSLARSTDFSKADATQAAALLEAYLDAAGYLDEADNPFSRAVELVDRTIA
                     PAIKKAEGGFFLQTDGDGRCDLLTGLYAGKALIRLSESSGKAIYAGIGQSLVTSAVKL
                     AAADGSIPASVTATGGMLTKSPERLSAAMIYPVVA"
BASE COUNT          274 a          631 c          611 g          295 t
ORIGIN      
        1 agtcgcccga gtccatcgag cggccgagcg cgtcgagcgc catccgctcc ttctcctcgg
       61 cgctcagctc ggccgggacg gtcgtatcca cggccgcgcc gggtagcgac ggaggctccg
      121 ccggcccggg gcggctccct aggcccgcgc aggaagcgag gagtataacg gctagggcgg
      181 cggccgagcg cgtcgccgtt gaaattctca tgcgtctctc cggtcgaagg gtcggattcc
      241 ggccatggta gccggtttat agcgctttag caagcgacgg agcgatggag cggccgccgg
      301 gacgggctcc cgcggttgcg caatagaact ggcaacgtgt atagtcccat cgatatgaga
      361 ataagcgcgt tcggaagggc cgcggcgatg gccttcgttt atatcgccgt cttcgtggtg
      421 ctcgtgatgg tccagttccc gtccgggggg cccatcaccg aggtgtcggg aggcgtatcg
      481 ttcaaggggt tgccggacgg cgacggcgta cgatccgcgg agctcgtcgc gaacggcctc
      541 aggctcgtct tctctgaacg ctacccgctg ctgttgagcg gtggcgcagg tccggacggc
      601 gcgttgtatc cggtagccta cgaggcagtc gatgacggtt tcatcgtccg gttcgacgac
      661 ggcacgagac tgttcgtcaa cgccgacgac gagggacggg cctcctggcg gctcgacgcc
      721 aagcgctcca agaaggcctc cgcgacgatg cgctacgagc tggcctatgg cgcggctctg
      781 ctcgccccgg gcgacgacgg ttccatacgg ctgagcttcg gcgacgctac ctaccgggta
      841 tccggcgtca ccaccggttc cgagccgcgc acgctgtcgc tcaaggcgac cggcggggcg
      901 ttcagggcct tcgcttcgat acgcgagacc aaggacgcgg cggagacccc ggcccagttc
      961 cttgcccagg ccccgatgga cccggccctc tggtccaggg agataggcgc ctggcgcgac
     1021 aaggcctggt cttccgtgtc gggcgccgat ttcgacgccg cgagcgggac ctggtccggc
     1081 gcgttcgacg agccgtcctt cgtcctgtac ctggcggagg ccatgcgccg cgacctgcgc
     1141 gacgccgccg cggccctggt cgccgtcgcg cgctcatcgc acgccgacag cctgagctgg
     1201 aagagcgcgc cgttcgccgg caagaccgct acctcgatgg ccgccttcga ggcggccaac
     1261 ctggcggagg tcaaggcgac cgagcggctc gtacaggccc gcgccggcac gatgttctac
     1321 cgtcgcggca tagtgccgct cctcttcgac cgcgccccct actcgctggc ccaggaagcc
     1381 atgtccttgg cccggtcgac cgacttctcc aaggccgacg ccacccaggc cgcggccctg
     1441 ctcgaggcct acctcgacgc ggccggctac ctcgacgaag ccgacaaccc cttcagccgg
     1501 gctgtcgagc tcgtcgacag gactatagcg ccggctatca agaaggccga gggcggattc
     1561 ttcctgcaga ccgacggcga cggccgttgc gacctcctaa ccggcctcta cgccgggaag
     1621 gccctgatcc gtctgtccga gtcgtcgggc aaggctatct acgctggcat aggccagagc
     1681 ctcgtgacga gcgccgtcaa gctggccgcg gccgacggct ccataccggc ctcggtcacg
     1741 gcgaccggag gcatgcttac caaatcgccc gagcggctct ctgccgcgat gatctatcca
     1801 gtcgtcgccg a
//
LOCUS       DAOOLN010000367         1802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_83429_length_1802_cov_3.331425, whole genome shotgun sequence.
ACCESSION   DAOOLN010000367 DAOOLN010000000
VERSION     DAOOLN010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83429_length_1802_cov_3.331425"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="PLW80_05460"
     CDS             complement(<1..973)
                     /locus_tag="PLW80_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HPB65987.1"
                     /translation="MTTYERRRHALAAKIADQNVAMAMFEDTEGRRDPSIRYFCGHPG
                     DALLFIAADGRSLLVPWDVNMAASMASVDAVAAYTDFSRSPFAAMAGALERLGVPGGS
                     VVDLPSVTPYPMYVDYVEACPDHDFRCSDDGSAKLAAEMRAVKDADEIATYRTVSAIT
                     DEAMDEIERGVRSGALATETDVALFIERFLRLKGCEATGFDTLAAGPSRSFGIHAFPA
                     YTAAAFATEGMSILDFGLSYKGYTSDVTMTFVRGSTAGRRGEMIALVQKAHDDAIAIC
                     GPGVRSRDVALLVDRVFEGKGYKMPHALGHGVGLEAHEAPAVRSREDN"
     gene            complement(997..>1802)
                     /locus_tag="PLW80_05465"
     CDS             complement(997..>1802)
                     /locus_tag="PLW80_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65988.1"
                     /translation="LIALGSMRADEYAERIALLLRDLNNEPTADKQGGEQVAYGCVIA
                     LERMRSPVGFAPLFFASEGWYSKRVRDQAERSLALVLADPSESVSALLRTETPPRMLR
                     ALALELRSQAPAVGKTAVAALALSRGIAYAPRNRVEQGQLSELRVVAMNALASAGPGN
                     GSAVPDMAEAYRIGPTDERLVAMKALGAEKSPASAAALRDIILDLNSAQKAGLVDETR
                     NALMRAALQNAAFNAGKELAPAVQAVLINGGWSSGVLSLASIAQKALQQ"
BASE COUNT          283 a          596 c          633 g          290 t
ORIGIN      
        1 cgttatcctc ccggctacgt acggccggag cctcgtgcgc ctcgagcccc acgccgtggc
       61 cgagcgcatg cggcatcttg tagcccttcc cctcgaacac gcggtcgacg agcagggcca
      121 catcgcggct cctgacgcct ggcccgcaga tcgctatggc gtcgtcgtgg gccttctgga
      181 cgagggctat catttcaccg cgcctaccgg cggtcgagcc ccggacgaag gtcatcgtca
      241 cgtccgaggt atagcccttg tacgacaggc cgaagtcgag gatggacatg ccctcggtgg
      301 cgaaggccgc cgcggtatac gcggggaagg cgtggatgcc gaagctgcgc gacggcccgg
      361 cggcgagggt atcgaagccg gtggcctcgc agcccttcag ccggaggaag cgctcgatga
      421 acagggccac gtcggtctcg gtggccagag cgccggaacg gacgcccctc tcgatctcat
      481 ccatcgcctc gtcggtgatg gcggagacgg tacggtaggt agcgatctcg tcggcgtcct
      541 tgacggcgcg catctcggcg gccagcttgg ccgacccgtc atcggagcat cggaagtcgt
      601 ggtcggggca ggcctcgacg taatccacgt acatcgggta gggcgtgacg gatggcaggt
      661 cgacgaccga gcccccgggg acgccgaggc gctccagggc gccggccatc gccgcgaagg
      721 ggctacggga gaaatccgta tacgccgcca ccgcgtcgac gctagccatc gaggcggcca
      781 tgttcacgtc ccatggcacg aggagggaac ggccgtcggc cgctatgaac aggagggcgt
      841 cacccggatg cccgcagaaa tagcggatgg aaggatcgcg ccggccctcc gtgtcctcga
      901 acatggccat cgcgacgttc tggtcggcga tctttgcggc caaggcatgg cgacgcctct
      961 cataggtagt catggtatac ctcctcaagc tcgtagttac tgctggagcg ccttctgggc
     1021 gatcgaggcg aggctgagca cgccgctcga ccagccgccg ttgatcagca cggcctggac
     1081 agcgggcgcc agctccttgc ccgcgttaaa ggcggcgttc tgcagcgcgg cccgcatcag
     1141 agcgttcctg gtctcgtcga cgagcccggc cttctgggcc gagttcaggt ccaggataat
     1201 atcacgtaat gccgcggccg aggcgggcga tttctccgcg ccgagtgcct tcatggcgac
     1261 gaggcgctcg tcggtcgggc cgatacggta ggcctctgcc atatccggca cggctgagcc
     1321 gttccctggc cccgcggagg ccagcgcgtt catggcgacg actcgtagct ccgacagctg
     1381 gccctgctcg acgcggttgc gaggcgcata ggcgatgccg cggctcaggg cgagcgctgc
     1441 gacggccgtt ttcccgacgg ccggagcctg ggagcgcaac tcgagcgcga gggcgcgtag
     1501 catgcgcggc ggcgtctcgg tacggagcag cgcggagacg gactcgctcg ggtcggcgag
     1561 cacgagggcc aggcttcgct cggcttggtc cctgacccgc ttcgagtacc atccctcgct
     1621 cgcgaagaat agtggggcga agcctaccgg cgagcgcata cgctcgagcg cgatgacgca
     1681 gccgtaggcc acctgctcgc cgccctgctt gtcggcggtc ggctcgttgt tcaggtcgcg
     1741 caggaggagc gcgatcctct cggcgtactc gtcggctcgc atcgagccga gcgctatcag
     1801 gg
//
LOCUS       DAOOLN010000368         1800 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_83581_length_1800_cov_3.285387, whole genome shotgun sequence.
ACCESSION   DAOOLN010000368 DAOOLN010000000
VERSION     DAOOLN010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83581_length_1800_cov_3.285387"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..649)
                     /gene="ssb"
                     /locus_tag="PLW80_05470"
     CDS             complement(173..649)
                     /gene="ssb"
                     /locus_tag="PLW80_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPB65989.1"
                     /translation="MADLSVAVLVGRLTRDAELKYTNSGQAVCHFSVATSSRKKKGDQ
                     WVDEASFWDVDLWGKQGESLNQYLVKGKLVAVEGTMRQDRWEQDGQSRMKVLISANTV
                     QLLGGNTSGGQQYGASQGSQDTQGGPREYQGRSGSRPQGPAAQAPASDDFSDDIPF"
     gene            complement(662..943)
                     /gene="rpsF"
                     /locus_tag="PLW80_05475"
     CDS             complement(662..943)
                     /gene="rpsF"
                     /locus_tag="PLW80_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPB65990.1"
                     /translation="MRSYELTVIFPTEEELYRQAKEKVSAALKEQGAEIVKEEELGDR
                     QLAYEIKDRMRGRYVLYVANIAPDKIVAVEKVLKLEPHVMKYLFVRAED"
     gene            1103..1546
                     /locus_tag="PLW80_05480"
     CDS             1103..1546
                     /locus_tag="PLW80_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65991.1"
                     /translation="MPHVIHYEDDIFIIDALVRTLCDASRLEPDPDVLGDAMLSIAKL
                     ADASLRKARDLVLLNEHQVDRPESLRLLARTTRSFSDALSELIRHDAPLGQHLASSSD
                     ELARMASAHRAAASELADVLRQATGEDASPEDHVSGDELSELLRL"
     gene            complement(1551..>1800)
                     /locus_tag="PLW80_05485"
     CDS             complement(1551..>1800)
                     /locus_tag="PLW80_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPB65992.1"
                     /translation="PLEAPASAMGRNTVEAIQAGVVLGYTGLVEYMVGRIKGELGGAK
                     VVATGGLCRVVSELTEVFDAIDPDLTLNGLAEMERYLF"
BASE COUNT          307 a          584 c          550 g          359 t
ORIGIN      
        1 gagcttctgc gagcagaatt tacagacctt cttgcggaag aagcctttgc ggccgccgcg
       61 gccgccgcgg tcgccgccgt cgcgccggcc ctcgtcgtta cgcgagccgc gatcgtccct
      121 gttctgcata tctgacatag agtgtctcct tacgtatgag tcccgttcta gcctagaacg
      181 gtatatcgtc cgaaaaatcg tcggacgccg gagcctgggc ggcgggtcct tgcggccggc
      241 tgcctgaacg gccctggtat tccctcggcc cgccctgagt atcctggctc ccctgcgaag
      301 ccccgtattg ctggccgcca gaggtgttgc ccccgagcag ctgtacggta ttcgcgctga
      361 tcagtacctt catgcgggac tggccgtcct gttcccagcg atcctggcgc atcgtgccct
      421 ctacggcgac gagcttgccc ttgaccaggt actggttgag ggattcgccc tgcttgcccc
      481 agaggtcgac gtcccagaaa ctggcttcgt cgacccattg gtcgcccttt ttcttcctcg
      541 agctggtggc taccgagaaa tgacagacgg cctgaccgga gttggtatat ttaagctccg
      601 cgtcgcgggt aagcctgcct acgagcacgg ctacgctgag atcggccata ggtccctccc
      661 gttagtcttc cgccctgacg aacaggtact tcatcacgtg cggctcgagc ttgagcacct
      721 tctcgacggc cacgatcttg tcgggcgcga tattggcgac gtacagcacg tagcggccgc
      781 gcatcctgtc cttgatctcg taggcgagct ggcggtcgcc gagctcttcc tctttgacga
      841 tctcggcgcc ctgctccttg agagcggcgg agaccttctc cttggcctgg cggtagagtt
      901 cctcttccgt cgggaaaata accgtgagtt cgtaagatcg catcgatcct ccctatggtc
      961 ttatgatccg cccgaaagga cggataagga gtcgcgcgcc ccgatgggcg cgcaaatcaa
     1021 gctactatat accggaaagg ccgacggtat caaggcttag gagacgcttt accctggcgc
     1081 gtggcagcgt tacaataact ccatgccgca tgtcatacac tacgaggacg atatctttat
     1141 catcgacgcc ctggtgcgta ccctatgcga cgcctcgcgg ctggaacccg atcccgacgt
     1201 gctcggcgac gcgatgctgt ccatagcgaa gctagccgac gcgtcgctcc gtaaggcgcg
     1261 cgaccttgtg ctgctgaacg agcaccaggt cgacaggccg gagagcctca gactgctggc
     1321 caggacgacc aggtcgtttt ccgacgcgct atcggagctg atccgacacg acgccccgct
     1381 agggcaacac ctagccagtt cgtcggacga gctggccaga atggcgtcgg cccaccgagc
     1441 ggcggcttca gagctggccg atgtactgcg tcaggctacc ggggaggacg cgtcgcccga
     1501 ggatcacgtg tcgggcgacg aattatcgga gctgctgcgc ctgtgacagc ctagaacagg
     1561 tagcgttcca tctcggcgag cccattgagg gtcaggtcgg ggtcgatggc gtcgaacacc
     1621 tccgtcagct cggagacgac gcggcagagg ccgccggtcg cgaccacctt ggctccgccg
     1681 agttctccct tgatgcggcc gaccatgtac tcgacgaggc cggtgtagcc gagcaccacg
     1741 ccggcctgga tggcctccac cgtgttgcgg cccatggccg aggccggcgc ctcgagcggc
//
LOCUS       DAOOLN010000369         1798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_83728_length_1798_cov_2.990247, whole genome shotgun sequence.
ACCESSION   DAOOLN010000369 DAOOLN010000000
VERSION     DAOOLN010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83728_length_1798_cov_2.990247"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="PLW80_05490"
     CDS             complement(<1..1017)
                     /locus_tag="PLW80_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65993.1"
                     /translation="MIDSSTMSNKRLALRVTRNSVVMIIAVYLVMQVFTYFRDGLILG
                     VSDLSDLLAMTAAYMFSVVIPPALVLGLILYLLALRIQRVACALERGETIPDDELERT
                     RKRLIGFSKVVLAENIVGFASGYVIFVVSQGFLHEILRFDRLVVLVSNLAGGVIYASA
                     QNALNEIVFADIRDRLGIREIGERRRERSTTGKQIGLSAALVVYAVTFIQFNVKDTVA
                     FNKVGDDILAGVVSGSILPEDAGTAYRSLLAERLPELGTRSSVGADDVPLPWERADSA
                     VRRQQTMFIMYAAFTLCICCAVQAIVSSSLRRQLRAIANRVRDVLDGGGDLRVRLSIR
                     AMDDL"
     gene            1063..1758
                     /locus_tag="PLW80_05495"
     CDS             1063..1758
                     /locus_tag="PLW80_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB65994.1"
                     /translation="MEPNTPPEDQAGGACVELDASALAGLRKMPVDVFDDVYLGEVKP
                     DALVLCVDVRNFSDFLCSHDEDEVFALIKDFTSNLLSCVNQFGYGCSYYKLMGDGAIV
                     IWDTFDEASAREALAVFDSYTDFVDEELFKPYPDLGLAGALVVEKVFKYEISAEASQL
                     KYRDYVGYGINLACRLQALARSGELAVNERLASSGLVTAVPDASSGILAELRSMKGLK
                     DEDRTRALLYRKA"
BASE COUNT          322 a          546 c          596 g          334 t
ORIGIN      
        1 gaggtcgtcc attgcgcgga tgctcaggcg gacgcgcagg tcgccgcctc cgtcgagcac
       61 gtccctgacg cgattggcta tcgcccgcag ctgtctgcgc aggctcgacg atacgatagc
      121 ctgcaccgcg cagcagatgc atagggtgaa ggcggcgtac atgatgaaca tcgtctgttg
      181 gcgcctgacg gcgctatcgg cgcgttccca gggtagcggt acgtcgtcgg ccccgacgct
      241 cgagcgtgtg ccgagctcgg gtagccgctc ggcgagtagc gagcggtagg cggtcccggc
      301 atcctccggc aggatggagc cggagacgac tccggctagt atatcgtcgc ccaccttgtt
      361 gaatgcgacc gtatccttga cgttgaactg gatgaaggtg accgcgtaga cgactaaggc
      421 ggccgatagc ccgatctgct tgccggtcgt cgagcgttct cgccggcgtt cgcctatctc
      481 gcggatgccg aggcggtcgc gtatatcggc gaagactatc tcgttcaggg cgttctgggc
      541 ggaggcgtag atcacgccgc cggctaggtt tgaaacgagc acgaccagcc tgtcgaagcg
      601 caggatctcg tgcaggaagc cctgcgacac gacgaagatc acgtaacccg acgcgaagcc
      661 tactatattc tctgcgagca cgaccttgga gaagccgatc aggcgcttcc tcgttcgctc
      721 aagttcatcg tccggaatgg tctcgccgcg ttcgagggca caggcaacgc gctggatgcg
      781 cagggccagc aggtacagga tcaggccgag taccagggcc ggggggatga cgacgctgaa
      841 catgtacgcg gccgtcatcg cgaggaggtc ggacaggtcc gagacgccga ggatcaggcc
      901 gtcgcggaag taggtgaata cctgcatgac gaggtagacc gctatgatca tcactacgga
      961 gttgcgggtt acgcgcaggg ctaggcgttt gttggacatg gtgctggagt ctatcacgga
     1021 agggcgtatc tgtggacggg tcctttctgg tagaatggcg tcatggaacc gaatacacct
     1081 ccggaggatc aggctggcgg cgcctgcgtc gagctcgatg cctccgccct ggcgggactc
     1141 aggaagatgc cggtcgacgt gttcgacgac gtctacctcg gcgaggtcaa gccggacgcc
     1201 ctcgtgttgt gcgtcgacgt gcgcaacttc agcgacttcc tctgttcgca cgacgaggac
     1261 gaggtcttcg ccctgatcaa ggatttcacc tcgaacctgc tgtcctgcgt caaccagttc
     1321 ggctacggtt gctcctatta taagctgatg ggcgacggcg cgatcgtcat atgggatacg
     1381 ttcgacgagg catccgcgcg agaggccctc gccgtcttcg attcctatac cgacttcgtg
     1441 gacgaggagc tgttcaagcc ctatcccgac ctgggactgg ccggggccct cgtcgtcgag
     1501 aaggtcttca agtacgagat ctccgccgag gcctcgcagc tcaagtaccg ggactacgtg
     1561 ggttacggca tcaacctggc ctgtcgcctc caggccctgg cgcgcagcgg cgagctagcc
     1621 gtcaacgagc gcctcgcctc gagcgggctc gttacggcgg tccccgacgc ctcgtcgggt
     1681 atcctggccg agctgcgctc catgaagggc ctcaaggacg aggacaggac ccgcgccctg
     1741 ctgtaccgca aggcctaggc gtccgcgttc ccgcgccgtc gtcgcatgaa ggcgagca
//
LOCUS       DAOOLN010000370         1796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_83880_length_1796_cov_3.177484, whole genome shotgun sequence.
ACCESSION   DAOOLN010000370 DAOOLN010000000
VERSION     DAOOLN010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83880_length_1796_cov_3.177484"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /gene="hydG"
                     /locus_tag="PLW80_05500"
     CDS             complement(<1..1309)
                     /gene="hydG"
                     /locus_tag="PLW80_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HPB65995.1"
                     /translation="MTTTTTRRETAAEWQRSLIRQDEIDRYLDDGRDFIDEPAIEATL
                     SRGVALAGDELRSRVRAAIDKAYTIKLMDDDDVAALLAVRDPDLRDEVAAAAEAIKAR
                     VYDNRVVTFAPLYCGSKCVNSCKYCGFRADNGAVERRVLTMDEIRAETKVLAGKIGHK
                     RLVVVYGEHPETDADYIADSMRAVYSVKAPARNGSGEIRRVNINAAPLPIDGLKKLAA
                     AGIGTYQVFQETYHKATYADVHPAGTLKGNYRWRLYALHRAMDAGIDDVAVGALFGLY
                     DWRFEVMGLVAHARDLERRYGIGPHTVSVPRLEPAAGTDFSWVRHWVSDDDFRYLVAV
                     LRLAIPYAGLIVTNRESPEMLRSVIRMCTQRDADARVGIGAYTEAFAPGAQRGENQQF
                     MLGDTRSLDAIVRELAGMGHIVSFCTAGYRCGRTGKKIMGLLES"
     gene            complement(1318..1569)
                     /locus_tag="PLW80_05505"
     CDS             complement(1318..1569)
                     /locus_tag="PLW80_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HPB65996.1"
                     /translation="MADRSVGVIAIIIKNRTAQAARVNDALSEYGDMIIGRMGLPYQE
                     RDLNIITLIVDATNDRIGALTGRLGQLPDVTVKSTLAKL"
BASE COUNT          279 a          580 c          612 g          325 t
ORIGIN      
        1 cggactcgag gaggcccatg atcttcttgc cggtgcggcc gcagcggtag ccggcggtgc
       61 agaacgacac gatgtggccc atgcccgcca gctcgcgcac tatcgcgtcg aggctgcgcg
      121 tgtcgccgag catgaactgc tggttctcgc cgcgctgggc ccccggcgcg aatgcctcgg
      181 tataggcgcc gatgccgact cgggcgtcgg cgtcgcgctg cgtgcacatg cgtatgaccg
      241 agcgcagcat ctccggcgat tcccggttgg tgacgatgag gccggcgtag ggtatggcga
      301 gcctgagcac ggctaccagg tagcggaagt cgtcgtcgct cacccagtgg cgcacccacg
      361 agaagtcggt gccggcggcc ggctcgagcc tcgggacgga gaccgtgtgc gggcctatgc
      421 cgtagcggcg ctcgaggtcg cgcgcgtgag cgacgaggcc catcacctcg aagcgccagt
      481 cgtacagccc gaacagcgcg ccgaccgcga cgtcgtcgat gccggcgtcc atcgcgcggt
      541 gaagcgcgta gagccgccag cggtagttgc ccttgagcgt gccggccggg tgcacgtcgg
      601 cgtaggtcgc cttgtggtag gtctcctgga agacctggta ggtgccgatg cccgcggccg
      661 ctagcttctt gaggccgtcg atgggcaggg gcgccgcgtt tatgttgacg cgcctgatct
      721 cgccagagcc attgcgcgcc ggcgccttga ccgagtagac ggcgcgcatg ctgtccgcta
      781 tgtagtcggc gtcggtctcg gggtgctcgc cgtagacgac gacgaggcgc ttgtggccta
      841 tcttcccggc gagcaccttc gtctcggccc ggatctcgtc catcgtgagc acgcgccgct
      901 cgaccgcccc gttgtcggcg cggaagccgc agtacttgca gctgttgacg cacttgctgc
      961 cgcagtacag cggcgcgaac gtgacgacgc gattatcgta gaccctggcc tttatggcct
     1021 ccgccgccgc ggcgacctcg tcgcggaggt ctggatcacg cacggccagg agggcggcaa
     1081 cgtcgtcatc gtccatcagc ttgatcgtat aggccttgtc tatcgcggcc cgtacccgcg
     1141 agcgtagctc gtcgccggcg agcgccacgc ctcgcgacag cgtcgcctcg atcgccggct
     1201 cgtctatgaa gtcgcggccg tcgtcgaggt agcggtcgat ctcgtcctgt cgaataaggg
     1261 agcgctgcca ttcggccgcg gtctcgcgtc gcgtagtcgt tgtagtcatc gcgcgcctca
     1321 cagcttcgcc agcgtcgact tgaccgtcac gtccggtagc tgtccgagcc tgccggtcag
     1381 ggcgcctatg cggtcgttcg tcgcgtctac gatgagcgtg atgatgttga ggtctcgctc
     1441 ctggtagggc aggcccatgc ggcctatgat catgtccccg tattcgctca gggcgtcatt
     1501 gaccctcgcc gcctgagccg tcctgttctt gatgatgatg gctatgacgc cgaccgacct
     1561 atcagccatg gaaacctcct ctaggataga aggcaccagg gtcaggacgg ggctacgggt
     1621 ataccgacca cgacaagggg ggagtcgagg aggcgtatcc ggcccttggc ttcagacggg
     1681 cgaaccgtcc tgaccgcagg tgcgcgatgg gcacagcgta gcacaattga atagtcctgg
     1741 caatatcgat acgcaaatac ccacaagacg cgacgagcgg atagacccgg acggcg
//
LOCUS       DAOOLN010000371         1796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_83886_length_1796_cov_2.851235, whole genome shotgun sequence.
ACCESSION   DAOOLN010000371 DAOOLN010000000
VERSION     DAOOLN010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83886_length_1796_cov_2.851235"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="PLW80_05510"
     CDS             <1..596
                     /locus_tag="PLW80_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HPB65997.1"
                     /translation="SVSAKPSRYEWADRRIVKFMLENYGLLPITTDSHFGEYISWASD
                     VVDHRGILDFFDVYKIMLSRAVERKIKLETTERVVPIIDGILGDEGFEESAVNVRNDG
                     AIADLPDWIAVEVPAVVGASGVNAIKVGELPKGYAALLRSYCGVYDLTAEAVIHKRRD
                     YAVQAILANPVVSKAAKVGELVDRMIAQQGRWLGYLR"
     gene            671..1723
                     /locus_tag="PLW80_05515"
     CDS             671..1723
                     /locus_tag="PLW80_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPB65998.1"
                     /translation="MATIKHIARLAGVSPTTVSNVLHGNTAKVSPAVRDRVLAIVAEE
                     NYAPNQGAVMLVRSRSRIIGVILFMEPRSGSTAIEDPFVSTIIGTLESEIRAAGYFIM
                     LRATSDKDEVLRLAATWKLAGLVMLCVPSEISSAIVRRIGSPVVFVDCYFADDGQSYH
                     SIRLDDEVGAYAMTNYLLSLGHRRFAFMANAADFPSVDSARYDGYRRAFAEAGLEAPA
                     DGLVTVPKDLDERLAMYRRLAAEPRPYSALFFSADFYAVEAIDYLSGLGVSVPGDLSI
                     VGFDDIWIARLSRPRLTTVHQDVVAKGKAASDMLMRLIHKEPVTDSDVRLPVRLEIRE
                     SAAESAGGPVSLPRER"
BASE COUNT          301 a          640 c          559 g          296 t
ORIGIN      
        1 agtccgtctc cgcgaagccg agccgctacg agtgggccga ccggcgcatc gtcaagttca
       61 tgctcgagaa ctacgggctc ctgcctatca cgaccgacag ccacttcggc gagtacatat
      121 cctgggcctc ggacgtggtc gaccaccgcg gcatcctcga cttcttcgac gtgtacaaga
      181 tcatgctgtc cagggccgtc gagcggaaga tcaagctcga gacgaccgag cgtgtcgtgc
      241 ccatcatcga cgggatactc ggagacgagg gcttcgagga atcggcggtc aacgtgcgca
      301 acgacggcgc catagccgac ctgccggact ggatagccgt cgaggtgccc gccgtcgtcg
      361 gcgcctcagg cgtgaacgct atcaaggtcg gcgagctgcc caagggctat gcggccctcc
      421 tgcgctcgta ctgcggcgtc tacgacctga cggccgaggc ggtcatccat aagcgccgcg
      481 actacgcggt ccaggccatc ctcgccaatc ccgtggtttc caaggcagcg aaggtcggcg
      541 agctcgtcga ccggatgata gcccagcagg gccgctggct tggctacttg cgctagaagc
      601 gcgttgtcga gcagtagacg cccctcgccg cggacggatc ctccgcccgc ggcgacgagg
      661 caccgatagc atggctacca tcaagcatat cgcccgcctc gcgggcgtca gccccacgac
      721 cgtctcgaac gtgctgcacg gcaataccgc caaggtgtcg cccgcggtgc gcgaccgcgt
      781 cctggccatc gtcgcggagg agaactacgc cccgaaccag ggcgcggtca tgctcgtgcg
      841 tagccgctcg cgcatcatcg gcgtcatcct gttcatggaa ccgcgctccg gtagcacggc
      901 catcgaggac cccttcgtct cgacgatcat aggcaccctc gagtcggaga tccgggcggc
      961 cggctacttc atcatgctgc gcgcaacctc ggacaaggat gaggtcctga ggctggcggc
     1021 cacctggaag ctggccggcc tcgtcatgct ctgcgtaccg agcgagatat cgagcgccat
     1081 cgtacgccgc atcggcagcc cggtcgtctt cgtcgactgc tacttcgccg acgacggcca
     1141 gtcctatcac tcgatacggc tcgacgacga ggtcggcgcc tatgccatga cgaactacct
     1201 gctgtcgctc gggcatcgcc gcttcgcctt catggccaac gcagccgatt tcccgagcgt
     1261 cgacagcgcc cgctacgacg gctaccgtcg cgccttcgcc gaggctggcc tcgaagcgcc
     1321 ggccgacggc ctcgtcaccg tgccgaagga cctcgacgag cgcctggcca tgtaccgtcg
     1381 gctggccgcc gaaccgcgcc cctatagcgc cctcttcttt tcggcggact tctacgccgt
     1441 cgaggcgata gactacctgt ccggcctcgg cgtctccgtg ccgggcgacc tgtccatcgt
     1501 cggcttcgac gatatctgga tcgcccgcct gtcgcgcccg cggctgacca ccgtacacca
     1561 ggacgtcgtg gccaagggca aagccgcctc cgacatgctg atgcggctca tccacaagga
     1621 acccgtcacc gactcggacg tgcgcctgcc cgtgcgcctc gagatacggg agtcggccgc
     1681 cgagtccgcc gggggccccg tatccttgcc gcgcgagcgc tgacgctcta gcgctccggg
     1741 gcgagcatag cgccgggtat atcgcgacaa cgcgggcaga ggccctctat gaagta
//
LOCUS       DAOOLN010000372         1795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_83941_length_1795_cov_3.806322, whole genome shotgun sequence.
ACCESSION   DAOOLN010000372 DAOOLN010000000
VERSION     DAOOLN010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83941_length_1795_cov_3.806322"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PLW80_05520"
     CDS             <1..280
                     /locus_tag="PLW80_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPB65999.1"
                     /translation="NLARALERLKEAGYWTYAADMGGQALPDADLPDRVALVLGAEGS
                     GVSRLLKERCDGALSIPQYGHVDSLNVSVAAGVFMYELRRRHGAPPSA"
     gene            complement(277..>1795)
                     /gene="pyrB"
                     /locus_tag="PLW80_05525"
     CDS             complement(277..>1795)
                     /gene="pyrB"
                     /locus_tag="PLW80_05525"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HPB66000.1"
                     /translation="DEQRYLYRKARELKTELRAGGDAAAFRIADPDYSAYLVFLENST
                     RTRESFRNAALFHRVRATMFDAATSSFAKNESLTDAIKMLVGYAPESCFVIRSKLEGV
                     CGWLADYLGLWAERSGYARPSFINAGDGKHEHPTQEFLDEFSFLEQLGWRDERIHLAL
                     IGDLRHGRTVHSKADGLRVFKEAVVDLVAPPDLAMPPEYVGKMRRNGFEVREWGSLDE
                     YLASGKAAAIWYFTRLQLERMGESVLEKAPALRRAVTFRRDMLDRLPEGARFYHPLPR
                     DRLAPTIPGFLDDTVLNAWDAQSANGYYTRAVQMAMLAGVLGDDFDGEGPAPRADDGA
                     FVVEAAIEPRRKPEYKVGIKPVDSGLVIDHIASGASIEEIWNRVDKIRRVLGLNLRSS
                     HGVYHTNSGAYKGIVSVPDLLSFGEKELKRLGAVSPGCTLNLVKDGAVVKKYRLGMPP
                     RIYNFDDIACRNENCVSHPSHQEHVETSFVRKSGTDTFVCRWCEREHSYSEIWRD"
BASE COUNT          268 a          598 c          635 g          294 t
ORIGIN      
        1 gaacctggcc cgagccctcg agcggctcaa ggaggccggc tactggacct acgcggccga
       61 catgggcggc caggccctgc ccgacgccga cctgcccgac cgcgtggccc tggtgctcgg
      121 cgcggagggc tccggcgtct cgcggctcct caaggagcgc tgcgacggcg cgctgtccat
      181 accgcagtac ggacacgtcg attcgctcaa cgtctcggtg gccgccggcg tgttcatgta
      241 cgagctgcgc cgccgccacg gcgcgccacc ttccgcctag tcgcgccaga tctccgagta
      301 cgagtgctcg cgctcgcacc agcggcagac aaaggtgtcg gtgccgctct tgcggacgaa
      361 cgaggtctcc acgtgctcct ggtgcgaggg gtgcgacacg caattctcgt tgcggcaggc
      421 tatgtcgtcg aagttgtaga tacgcggcgg catgcccagg cggtacttct tgaccacggc
      481 gccgtccttg acgaggttga gcgtgcagcc gggcgatacc gccccgagcc gcttgagctc
      541 cttctcgccg aagctcagga ggtcgggtac cgacacgatg cccttgtagg cgcccgagtt
      601 ggtatggtag acgccgtgcg acgagcgcag gttgaggccg agcacgcggc gtatcttgtc
      661 gacgcggttc cagatctcct cgatggacgc gcccgaggcg atatggtcga tgacgaggcc
      721 ggagtcgacc ggcttgatgc cgaccttgta ctcgggcttg cgcctcggct cgatggcggc
      781 ctcgacgacg aaggccccgt cgtcggcccg cggagcgggc ccctcgccgt cgaaatcatc
      841 gcccagcacg ccggcgagca tggccatctg cacggcccgg gtgtagtagc cgttggccga
      901 ctgggcgtcc caggcgttga gcaccgtgtc gtcgaggaag cccgggatgg tcggcgccag
      961 gcggtcgcgc ggcaaggggt ggtagaagcg cgcgccttcg ggcaggcggt cgagcatatc
     1021 cctgcggaag gtgacggccc ggcgcagggc aggcgccttc tcgagcaccg actcgcccat
     1081 acgctcgagc tgcagccgcg tgaagtacca gatggccgcg gctttgcccg aggccaggta
     1141 ctcgtccagg gagccccact cgcgcacctc gaagccgttc cggcgcatct tgccgacgta
     1201 ctcgggcggc atagccaggt cgggcggcgc caccaggtcg acgacggcct ccttgaagac
     1261 ccgtagcccg tcggccttgg agtggacggt gcggccgtgg cggaggtcgc cgatcagcgc
     1321 gaggtggatg cgctcgtcgc gccagccgag ctgctcgagg aagctgaact cgtcgaggaa
     1381 ttcctgggtc gggtgctcgt gcttgccgtc gccggcgttg atgaacgacg gcctcgcgta
     1441 gccggagcgc tcggcccaga gcccgagata gtcggccagc cagccgcaga cgccctcgag
     1501 cttggaacgt atgacgaagc acgactcggg cgcgtagccg acgagcatct tgatcgcgtc
     1561 cgtcaggctc tcgttcttgg cgaagctcga ggtggccgcg tcgaacatgg tggcccggac
     1621 gcggtggaac agcgccgcgt tgcggaagct ctccctggtc cgggtcgagt tctcgaggaa
     1681 cacgaggtag gccgagtaat ccgggtcggc tatcctgaag gccgccgcgt cgccccccgc
     1741 tcgtagctcg gtcttcagtt cccgagcctt gcggtacagg tatcgctgct cgtcg
//
LOCUS       DAOOLN010000373         1795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_83946_length_1795_cov_3.457471, whole genome shotgun sequence.
ACCESSION   DAOOLN010000373 DAOOLN010000000
VERSION     DAOOLN010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83946_length_1795_cov_3.457471"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            447..1645
                     /locus_tag="PLW80_05530"
                     /pseudo
     CDS             447..1645
                     /locus_tag="PLW80_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013384.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophanase"
BASE COUNT          367 a          637 c          514 g          277 t
ORIGIN      
        1 ctttgacgac aaccctactg agcttgaaac atcatagatt cctaacatac gctcgtccag
       61 caaattcctc atcatcaaac accgaacgga tcgaaaacga ggagcgccaa tcccggagtt
      121 gccgaccccg ctccatggcg tttctataaa taactacaat acaatgaatt attcggtttt
      181 gccctttgac taatcctcga gggcgggagt agaataccgc gtcccgcaac cagcgggcgc
      241 gaggagccac catgaacctg ccctgggccg agccctaccg gatcaagacc atcgagccga
      301 tacgccgatc gacgcgcgcc gagcgcgagt cgtggatagg aagcgccaag tacaacctgt
      361 tcaacctgcg gagcgagcag gtctacgtcg acctcatcac cgattccggt accggcgcca
      421 tgtcgagcgc ccagtgggcc gagatgatgc tcggcgacga gagctacgcc ggcgcgacct
      481 cctactacaa gctgaagaga ccatcgagac cgtcttcggc ttcccctact tcctgccgac
      541 ccaccaggga cgcgcggccg agaacgtgct cttctccgct atgatcggtt ccggccccga
      601 gtcgggtaag ggcctgatca tccccggcaa cagccacttc gacacgacca agggccacat
      661 cgagttccgc ggcgcccacg ccgtggactg caccatcgac gaggccttcg atacgcaggt
      721 ggagcacccg ttcaagggaa acgtcgacct caagaagctc gacgccgccc tgtcgcgcaa
      781 ggacgtgaag gcgccgctct gcctcgtcac cataacctgc aacagctcgg gcggccagcc
      841 cgtcagcatg gccaacctcc gcgaggtctc cgcgctctgc cgccagcata aggtgccggt
      901 cttcttcgac tcggcccgct tcgccgagaa cgcgtacttc atcaagacgc gcgagccggg
      961 ctacgccgac aagagcatcc ccgccatcgt caaggagatg ttctcctacg ccgacggctg
     1021 caccatgtcg agcaagaagg acggcatcgt caacatcggc ggcttcatcg gcttccgtag
     1081 ccaggaggca tggcgcaagg cgagcacctt caacatcatg ttcgaaggct tcaacaccta
     1141 cggcggcatg gccggccgcg atatgggagc cctctccgtc ggcctccgcg aggcctgcga
     1201 actcgactac ctcgagtcgc gcgtcggcca ggtcgcctac ctgggccagc ggctcaccga
     1261 ctacggcatc gccgtccagc gcccctacgg cgggcacgcc atcttcgtcg acgcgaagaa
     1321 gttcctgccc aaggtgccca aggaggagtt ccaggcccag acgctcggcg tcgagctgta
     1381 cctcgaggcc ggtgtgcgcg gcgtcgagat aggcgcgctg ctcgccgacc gcgacccgga
     1441 cacccacgag aaccgatacc cggccctcga gctcctgcgc ctcgcgatac cgcgccgcgt
     1501 ctacacgaac gcgcacatgg actacgtggc ggccgccctc gccaacgtga aggaacgcgc
     1561 cgacggcatc aggcgcgggt accgcgtcgc ctgggaagcc cccatcatgc gccacttcac
     1621 cgtagagcta acgccagcaa aatagcctat ccggccgccg ccacgcaccg aacatgaagt
     1681 ggcggcgcgc cagggttgga gcgggccgct ttcagcggcc acgaccatca tttcctatcg
     1741 gaatccgggc atgcaccgca tgcccggatg gcgcgcccaa gggcggcccg tccgc
//
LOCUS       DAOOLN010000374         1795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_83962_length_1795_cov_2.654023, whole genome shotgun sequence.
ACCESSION   DAOOLN010000374 DAOOLN010000000
VERSION     DAOOLN010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83962_length_1795_cov_2.654023"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PLW80_05535"
     CDS             <1..190
                     /locus_tag="PLW80_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66001.1"
                     /translation="TYTAGGQSASQAGPWINVDGPTYYGAIYPSSNAFYLAAEGLSGT
                     GTWTIDLPEGVYLTVVIK"
     gene            complement(166..>435)
                     /locus_tag="PLW80_05540"
     CDS             complement(166..>435)
                     /locus_tag="PLW80_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HPB66002.1"
                     /translation="RTQYINVKAFGSLVNRVLPVKIRGDGVVHTVLSEHYMFEMASED
                     YAVAGDLLSGYGVKLIQQFSGTGYNDRVRILSDYGSRIYLITTVR"
     assembly_gap    436..445
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<446..>1795)
                     /locus_tag="PLW80_05545"
     CDS             complement(<446..>1795)
                     /locus_tag="PLW80_05545"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HPB66003.1"
                     /translation="VMLAAPDCGESGGECYASNLVYSGNHAEIVERTYQGKTRLLTGV
                     NPSGFEWLLEPGESFSTPEAVLSYSAEGFNGLSANLHRFVRDRIVRGEWQYRERPVLV
                     NNWEATDFDFDRRKLLALAKEAADLGIELFVLDDGWFGHRDDDSSSLGDWEVNEKKLP
                     GGIAGLAGKVNALGLKFGLWVEPEMISEDSDLYRARPDWAVSTPGRAPAYGRNQFVLD
                     LTIPEVRAYLIEAMTKAFSQARIEYVKWDMNRNISDGYSKALPPERQGEFHHRYILGL
                     YEVLAELTKRFPGILFESCASGGNRFDLGMLCYMPQTWTSDDTDAYARQSIQGGTSYG
                     YPPSVMGAHVSASPNMQTLRASTIETRFNVAAFGLLGYELDLTMLSTFDKKAIKAQVA
                     WYKEHRRLLQFGRFIRSRSRLDERKIVWTVVSDDGREAIAGLFQGPATVGLGQDLLAV
                     "
BASE COUNT          282 a          588 c          598 g          317 t
ORIGIN      
        1 tacgtatacc gcgggcgggc agagcgcctc gcaggctggg ccgtggataa acgttgacgg
       61 cccgacctac tacggcgcca tctacccgtc gtcgaacgct ttctacctgg ccgccgaggg
      121 gctgtcaggc accgggacct ggacgatcga cctgccggaa ggcgtctacc tgaccgtcgt
      181 gatcaagtag atgcgcgagc cgtagtccga caggatgcgc acgcggtcgt tgtagccggt
      241 gccggagaac tgctggatca gcttgacgcc gtagcctgac agcaggtcgc cggcgacggc
      301 gtagtcctcg gaggccatct cgaacatgta gtgctcggac agcacggtgt gcaccacgcc
      361 gtcgccgcgt atctttacgg gcagcacgcg gttgaccagg ctgccgaagg ccttcacgtt
      421 gatgtactga gtccgnnnnn nnnnncacgg cgagcaggtc ctgcccgagt ccgaccgtcg
      481 ccggtccctg gaagaggccg gctatcgcct cgcggccgtc gtcggagacg acggtccaga
      541 ctatcttgcg ctcgtcgagc ctggagcgcg agcggatgaa gcgcccgaac tgcaggagcc
      601 tcctgtgctc cttgtaccac gcgacctgcg ccttgatcgc cttcttgtcg aacgtcgaca
      661 gcatcgtcag gtccagctcg tagcccagga ggccgaacgc ggccacgttg aagcgcgtct
      721 ctatcgtcga ggcgcgcagg gtctgcatgt tcgggctggc cgacacgtgc gcgcccatca
      781 cgctcggcgg gtagccgtac gaggtaccgc cctggatgct ctggcgggcg tacgcgtcgg
      841 tgtcgtcgct ggtccaggtc tggggcatgt agcagagcat gccgaggtcg aagcggttgc
      901 ctcccgacgc gcaggactcg aacagtatgc cggggaagcg cttcgtcagc tcggcgagca
      961 cttcgtagag gccgagtata tagcggtggt ggaactcgcc ctggcgctcc ggcggcaggg
     1021 ccttcgagta gccgtcggag atattacggt tcatatccca tttgacgtac tcgatcctgg
     1081 cctgcgagaa ggccttggtc atcgcctcga tcaggtatgc gcggacctcc ggtatcgtca
     1141 ggtcgagcac gaactggttg cggccgtacg cgggcgcgcg gcccggcgtc gagacggccc
     1201 agtcggggcg ggcgcggtac aggtccgagt cctcgcttat catctccggc tcgacccaca
     1261 gcccgaactt gaggccgagc gcgttgacct tcccggcgag gcccgcgatg cctccgggca
     1321 gcttcttctc gttgacctcc cagtcgccga gcgagctcga gtcgtcgtcc ctatggccga
     1381 accagccgtc gtcgagcacg aacagctcga tccccaggtc ggccgcctcc ttcgcgagcg
     1441 ccagtagctt gcgcctgtcg aagtcgaagt cggtcgcctc ccagttgttg acgagcacgg
     1501 gccgctcgcg gtactgccac tctccgcgca cgatccggtc gcggacgaag cggtgcaggt
     1561 tggcgctgag cccgttgaat ccctcggccg aatagctgag caccgcctcc ggcgtcgaga
     1621 aggactcgcc cggctcgagc aaccactcga atccggacgg gttgacgccg gtcaggaggc
     1681 gggtcttgcc ctggtaggtc cgctcgacta tctcggcgtg gttgcccgag tagacgaggt
     1741 tgctcgcgta gcactcgccg ccggactcgc cgcagtccgg ggccgccagc atgac
//
LOCUS       DAOOLN010000375         1793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_84083_length_1793_cov_2.964327, whole genome shotgun sequence.
ACCESSION   DAOOLN010000375 DAOOLN010000000
VERSION     DAOOLN010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84083_length_1793_cov_2.964327"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..>1793
                     /locus_tag="PLW80_05550"
     CDS             68..>1793
                     /locus_tag="PLW80_05550"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HPB66004.1"
                     /translation="MNSLRLETLSKRLAGHPPLAEALRRIDEGSFPVDCSELEQPLAA
                     LMTAEAARMSGGSRRYFVVTPTDAEAEAFVDDCRAAGAEAMVFPWWSAAAYRPVEPRS
                     AAFGQRAAALAAVLDDHGPRVIVMGQRAAMTPVPPRSAFERGLFRVRKGDEIDAGALA
                     DRLAAWGYLRVPRVSLPGEFALRGEVLDVFMSGDESALRIVFEYDEVEEIRRFEPVSQ
                     ASDPERPDTVLLRPSRECVWTPELAARVGEALAACPGPSGEDRSLAIASVVESLTELG
                     EAKGEELLYPLAYGGPSSLFGYLGPDDVVVLLERERLLGQSEAVKREYGGLYRKALLE
                     GPVPPPEALLVDFEREAAALSRVLSSWSLKDESAGDRLAFGSEPPRSFFGNVNYFRDE
                     LDSLLRAGYEVRVFAENAVQAERIASLIARDEVAVHPSGLSAGFSVPQAKLAFVHEGE
                     IFGRRKRAPKSLKSVRSKVIDTFVELNPGDFVVHVNYGIGRFVGIERVKVLGHDRDYV
                     KIEYADEESVFVPIEQSNLVQRYIGNEGDEPRMDRLGSKSWENRKNKVKKSVEDLAER
                     LIRIYSRRK"
BASE COUNT          263 a          608 c          630 g          292 t
ORIGIN      
        1 tgcgcgccgc cgcgattcgt gtactatggt cggcgcgcta tcctccttca aatatcggac
       61 gttttatatg aactccttac gccttgaaac gctatcgaaa cgactcgcgg gccatccgcc
      121 gttggccgag gcccttcggc gcatcgacga aggctccttc ccggtggact gctcggagct
      181 ggagcaaccg ctcgcggcgc tcatgaccgc cgaggcggcc aggatgagcg gcgggtcccg
      241 gcgctacttc gtcgtgacgc cgaccgacgc cgaggcggag gctttcgtcg acgactgccg
      301 cgccgccggc gccgaggcga tggtgttccc ttggtggagc gccgcggcct accggccggt
      361 cgagccgcgc tcggcggcct tcggccaacg ggcggcggcc ctcgcggcgg tgcttgacga
      421 ccacggtccg cgcgtcatcg tcatgggcca gcgcgccgcc atgacgccgg tgccgcctcg
      481 ctcggccttc gagcgaggcc tttttcgggt acgcaagggc gacgagatag atgccggcgc
      541 gctcgccgac cggcttgcgg cctggggcta cctgcgcgta ccgcgcgtga gccttcccgg
      601 cgagttcgcc ttgcgcggcg aggtgctcga tgtcttcatg tcgggcgacg agagcgcgct
      661 caggatagtc ttcgagtacg acgaggtcga ggagattcgc aggttcgagc cggtgtcgca
      721 ggcctccgac ccggagcgtc ccgatacggt gctgctcagg ccgtcgcggg agtgcgtctg
      781 gacgccggag ctggcggccc gcgtcggcga ggcgctcgcg gcctgtcccg gcccctcggg
      841 cgaggaccgc tcgctggcca tagcgtccgt cgtcgagtcg ctgaccgagc tcggcgaggc
      901 caagggcgag gagctcctgt acccgctcgc ctacggcggc ccgtcgagcc tgttcggcta
      961 tctcgggcca gacgacgtcg tcgtgctgct cgagcgggag cgcctcttgg gtcagagcga
     1021 ggcggtcaag cgcgagtacg gcggcctgta ccgcaaggcc ctgctcgagg gaccggtgcc
     1081 gccgcccgaa gccctgcttg tcgacttcga gcgcgaggcg gccgcgctgt cccgggtcct
     1141 ctcgagctgg tcgctcaagg acgagtcggc gggcgaccgg ctcgccttcg gctccgagcc
     1201 gccccggtcc ttcttcggca acgtcaacta cttccgcgac gagctcgact cgctgctgcg
     1261 ggccggctac gaggtacgcg tgttcgccga gaacgcggtg caggctgagc gtatcgcctc
     1321 gctgatcgcg cgcgacgagg tggcagtgca cccgagcggg ctgtcggccg gcttctccgt
     1381 gccgcaggcc aagctggcct tcgtgcacga gggtgagatc ttcgggcgcc gcaagcgcgc
     1441 gcccaagtcg ctcaagagcg tacggtcgaa ggtgatcgac accttcgtcg agctcaaccc
     1501 gggcgacttc gtcgtgcacg tcaactacgg catcggccgc ttcgtcggca tcgagcgcgt
     1561 caaggtgctc ggccacgacc gcgactacgt caagatagag tacgccgacg aggagagcgt
     1621 cttcgtcccg atcgagcagt cgaacctggt ccaacgctac atcggcaacg agggcgacga
     1681 gccgaggatg gaccggctcg gctccaaatc ctgggagaac cgcaagaaca aggtcaagaa
     1741 gtcggtcgag gacctcgccg agcggctgat acgcatctac tcgaggcgca agc
//
LOCUS       DAOOLN010000376         1792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_84144_length_1792_cov_3.466897, whole genome shotgun sequence.
ACCESSION   DAOOLN010000376 DAOOLN010000000
VERSION     DAOOLN010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84144_length_1792_cov_3.466897"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PLW80_05555"
     CDS             complement(<1..669)
                     /locus_tag="PLW80_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB66005.1"
                     /translation="MSAAETGKGGRAAPDIKPTQLLGRGPGGRGPGGHGPGGFGMPGE
                     KAKDFKGTTRRLVSYLRPHLPAILFALFMAVLSTVFSIAGPKILGQAITLLFEGVARS
                     AVDFRGIGRILALLLGLYLVSAGFNYLTQYSMAGVSQKTVYSFRKGIDEKLARLPLRY
                     FDGRTHGEILSRVTNDVDTIANTLQQSLVQIITSAVTLVGSVVMMLTISWKLTLVALA
                     TLPFY"
     gene            complement(666..>1792)
                     /locus_tag="PLW80_05560"
     CDS             complement(666..>1792)
                     /locus_tag="PLW80_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB66006.1"
                     /translation="IRVVRAFDRVEHERARFDGVNADLTATSLKVNRIMAFVPAFITV
                     MMNLTSVMIVWFGGREVGSGAIRIGSLMAFIQYAAHIMFSVVMVSVIFIMVPRAQVSA
                     ERVNEVLDVEPEIDDPASPVRPVEAKGRVEFRNVGFGYDGASEPAVKDVSFVAEPGTT
                     TAIIGGTGSGKSTLINLLARFYDVKDGAVLVDGVDVRDMSQETLRSYLGLAPQNATLF
                     SGTIAENLRFGRDEATDDELAEAAAIAQASAFIAEKDGGMEATLSQGGVNVSGGQKQR
                     LSIARALARKPKIYLFDDSFSALDFKTDARLRSALRKKTGDATVIIVAQRVGTIRDAD
                     QIVVLDEGRVVGKGRHAELMATCAVYKEIVASQLSAEEAV"
BASE COUNT          298 a          590 c          596 g          308 t
ORIGIN      
        1 gtagaagggc agggtggcca gagcgacgag cgtcagcttc cagcttatgg tcagcatcat
       61 gacgacgcta ccgaccagag tcacggccga ggtgatgatc tgcacgaggc tctgttgcag
      121 ggtgttggcg atggtgtcga cgtcgttggt cacacgggac aggatctcgc cgtgggtacg
      181 gccgtcgaag tacctgagag gcaggcgggc cagcttctcg tcgatcccct tgcggaagga
      241 gtagaccgtc ttctgggaga cgccggccat cgagtactgg gtcaggtagt tgaagccagc
      301 cgagaccagg tacagcccca ggagcagcgc cagtatcctg cctatgcccc tgaagtcgac
      361 ggctgacctg gcgacgccct cgaacagcag cgtgatggcc tgaccgagaa tcttggggcc
      421 ggctatcgag aacacggtac tcaggacggc catgaacagc gcgaacagga tggccgggag
      481 gtgtggccga aggtaggata ccagccgcct cgtcgtgccc ttgaagtcct tggccttttc
      541 tccgggcatg ccgaaacctc ccgggccgtg gcctccggga cctcgcccgc ccgggcccct
      601 gccgagcagc tgggtcggct tgatatcggg cgcggcccgc ccgcccttcc ccgtctccgc
      661 ggcgctcata ccgcctcctc cgccgacaac tgcgacgcta cgatttcctt gtacacggcg
      721 caggtcgcca tcagctcggc gtgcctgccc ttgcccacga cgcggccctc gtcgagcacc
      781 acgatctggt cggcgtcgcg tatggtgccg acgcgctgcg cgacgatgat gaccgtcgcg
      841 tctccggtct tcttccgtaa ggccgagcgc aagcgcgcgt cggtcttgaa gtcaagggcg
      901 gagaagctgt cgtcgaacag gtatatcttg ggcttccgcg ccagcgccct cgcgatcgag
      961 aggcgctgtt tctggccgcc cgagacgttc acgccgccct gggacagggt cgcctccatg
     1021 ccgccgtcct tctccgctat gaacgcggag gcctgggcaa tcgccgcggc ctctgccagc
     1081 tcgtcgtctg tggcctcgtc cctgccgaag cgcaggttct cggcgatggt gccggagaat
     1141 agggtggcat tctggggcgc caggcccagg taggagcgta gcgtttcctg gctcatgtct
     1201 cgcacatcga cgccgtcgac gaggaccgcc ccgtccttga cgtcgtagaa gcgggcgagc
     1261 agattgatca acgtcgactt gcccgagccc gtgccgccta tgatggccgt ggtcgtgccc
     1321 ggctccgcga cgaaggacac gtccttgacg gcgggctcgg acgcgccgtc gtagccgaag
     1381 ccgacgttcc tgaactcgac gcggcccttg gcctcgaccg gcctgaccgg ggaggccggg
     1441 tcgtcgatct cgggctcgac gtcgagcacc tcgttgacgc gctcggccga gacctgggcg
     1501 cgcggcacca tgatgaagat caccgagacc atgaccaccg agaacatgat gtgggccgcg
     1561 tactggatga aggccatcaa cgagccgatc cggatggcgc ccgagcctac ctcgcgcccg
     1621 ccgaaccaga ctatcatcac cgaggtcagg ttcatcatca ccgtgatgaa ggccggcacg
     1681 aaggccatga tgcggttgac cttgaggctg gtggcagtca agtcggcgtt gacgccgtcg
     1741 aagcgggccc gctcgtgctc gacccggtcg aaggcgcgca cgacccggat gc
//
LOCUS       DAOOLN010000377         1791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_84252_length_1791_cov_2.134793, whole genome shotgun sequence.
ACCESSION   DAOOLN010000377 DAOOLN010000000
VERSION     DAOOLN010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84252_length_1791_cov_2.134793"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PLW80_05565"
     CDS             <1..201
                     /locus_tag="PLW80_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66007.1"
                     /translation="ATDCKSVQSGSTPDRASKNTDANQQDSRHRSGFFVIRRAMSAQK
                     SVLLLPEFVALLPLDGFIYPSR"
     gene            218..652
                     /locus_tag="PLW80_05570"
     CDS             218..652
                     /locus_tag="PLW80_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66008.1"
                     /translation="MASIQKRGDKYQVRIKHRLLPKPLFVTLDTEDAASAYGADYVGL
                     VGMPTGMGVEVVDACALLEAAAEADSAYDREHVCPFLYCRPERFKSIRPECPPSYRLP
                     EARVTVDTAEDYERAVAIVRELGNEPSDEAVIAWLLSRREGR"
     gene            658..>1791
                     /locus_tag="PLW80_05575"
     CDS             658..>1791
                     /locus_tag="PLW80_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66009.1"
                     /translation="MVEILLVPSVRRGNGSGHLVRCFSLAAALGPKAAVYLADEPGPG
                     SWSADELRLAYPRETASTRIIGAIRSDARFRLVVLDRRRTNQDELERWIGIGAVAAID
                     EGGPARGSAHYLVDILPRIAGATRGRGVDAANLASLGFLALPVARREPPTRAGRVLVS
                     FGGEDPAGLADAFLSAALSSGLVTPQAVSVVSGALSGAAAEYEGVTALGPVQNLKEKL
                     RSYDLVVTQFGLTAFEAAWAGCAVLLLNPSDEHERLGRAVGFASLGVGRPNSRRLRAA
                     FSDIGGLAAASAAAAPPERLDLAAKLAELRPHHAGECPVCGRRLGEAIYRSERKTYLR
                     CPDCGLVRMTHFFPRENPYAERAYFFDEYKAQYGRTYIDDIPSI"
BASE COUNT          289 a          624 c          593 g          285 t
ORIGIN      
        1 gcaacggatt gcaaatccgt ccagtccggt tcgactccgg atcgcgcctc caaaaatacc
       61 gatgcaaatc aacaagatag ccgccatcga agcggctttt ttgtcatccg tcgcgccatg
      121 agtgcacaaa aatccgtgct gttgctacca gaattcgtag cattgctacc gttagacgga
      181 ttcatttatc cgtcgaggtg agttagcatc cgcccccatg gcatccattc aaaagcgggg
      241 cgacaagtac caggtgcgca tcaagcaccg cctgctgccc aagcccctgt ttgtcacgct
      301 cgataccgag gacgcggcca gtgcctacgg cgccgattat gtcgggctcg tcggcatgcc
      361 gaccggcatg ggcgtcgagg tggtcgacgc gtgcgccctg ctcgaggcgg cggcggaggc
      421 cgactccgcc tacgaccgcg aacacgtctg ccccttcctg tactgccgcc ccgagcgctt
      481 caagtcgatc aggccggaat gtccgccttc gtaccgcctg ccagaggcgc gggtcacggt
      541 cgacacggcc gaggactacg agcgggccgt cgccatagtc cgcgagctag ggaacgaacc
      601 gagcgacgaa gccgttatag cgtggcttct ctcgcgccga gagggtaggt agtcgccatg
      661 gtagagatac tcctcgtacc gtcggttcgc cgaggcaacg gttccggcca cctggtgcgc
      721 tgtttctcgc tggcggcggc actgggaccg aaggcagccg tctacctggc cgacgagccc
      781 ggcccgggct cgtggagcgc cgacgagctc aggctggcct atccgcgcga gaccgcgtcc
      841 acgaggatca tcggcgccat ccgctccgac gcgcgcttca ggctcgtcgt gctggaccgg
      901 cgccgtacga accaggacga gctggaacga tggataggca tcggggccgt cgccgctatc
      961 gacgagggcg gcccggcgcg cgggtcggcc cactacctcg tcgacatcct gccgaggata
     1021 gccggggcca cgagagggcg gggcgtcgac gcggccaacc tggcgtcgct cggcttcctg
     1081 gccctgcccg tggcgcgcag agagccgccg acccgcgccg gccgcgtgct cgtcagcttc
     1141 ggcggcgagg atccggccgg attggcggac gcgttcctct cggcggccct gagcagcggg
     1201 ctggtaacgc cccaggccgt cagcgtcgtt tccggcgccc tgtccggagc cgctgcggag
     1261 tacgagggcg tgaccgccct cggcccggtc cagaatctca aggagaagct ccggtcctac
     1321 gaccttgtcg ttacgcagtt cggcctgacc gcattcgagg cggcctgggc cggctgcgcg
     1381 gtgctgctcc tcaacccgag cgacgaacac gaacggctcg gcagggccgt cgggttcgcg
     1441 agcctcggcg tcggccgccc gaactcacgc cgcctgaggg ccgctttctc cgacatcggc
     1501 ggcctcgcgg cggcgtcggc cgcggcggcg cctccggagc ggctcgacct ggccgcgaag
     1561 ctcgccgagc tgaggcccca tcacgcgggg gaatgccccg tctgcggccg gcgcctcggc
     1621 gaggcgatct atcgctcgga gcgcaagacc tacctgcgct gcccggactg cgggctggtc
     1681 aggatgacgc atttcttccc gcgggagaac ccgtacgccg aacgggccta cttcttcgac
     1741 gagtacaagg cccaatacgg ccgcacctac atcgacgaca tcccgagcat a
//
LOCUS       DAOOLN010000378         1788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_84462_length_1788_cov_2.818811, whole genome shotgun sequence.
ACCESSION   DAOOLN010000378 DAOOLN010000000
VERSION     DAOOLN010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84462_length_1788_cov_2.818811"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..1764
                     /locus_tag="PLW80_05580"
     CDS             304..1764
                     /locus_tag="PLW80_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HPB66010.1"
                     /translation="MAYDDLQDYMAALESKGLLRRVGVEVDPELEITEIADRVMRQPG
                     GGPALLFEKVKGSPYPLLINAMGSEERMAMAFGASSLDEKAAEIEDLIAWAWKQLRGF
                     NILKAVPEALPKLPVALSLFPRRVSRPKCQELVSDEEGFSSLPVLKCWPLDGGRFLTL
                     PLVCTRDPETGVQNVGMYRMQVYDDRTAGMHWHLHKDGAHFFQKYQARGERMPVAVAL
                     GCDPAVTYASTAPLPEGVWEMLFAGFLRGKSAEITKATLSGLDVPADAEFILEGYVDP
                     SEVRVEGPFGDHTGFYSLEDEYPVFHLERITRRRSPVYPATIVGIPPKEDCWMAKATE
                     RLFLPLLKQLCPEIVDLSMPLEGVFHNCVIVSIKKRFPGQARKVMNFMWGMGQMMYTK
                     LIVVVDDDVKPSDVSTVAWRAFNNIDAKRDLVFSDGPLDALDHSSPLPRYGTRLGIDA
                     TRKGPEEGHTRPWPSPLAMDEHIKSLVDGRWEQYGL"
BASE COUNT          306 a          626 c          569 g          287 t
ORIGIN      
        1 tccggcggcg gcgaccgcgt cgcgtatcgc ctcggacgac ctgaacagac cggtcagctc
       61 ctcacggaac gcgaaggccg cgagcgcgac ggccgcgatg gccgccggaa agccggctat
      121 cgccagcgcg cgacgaaagc gttcccgtcc catgcctcga ctatacgccg tatcgcctcg
      181 tttgtggaga ggctctatgc tatagcgtac gacgccgtgg cgcagttcga gcctggtgcc
      241 ctagccgcgg cctcccggcc gtcctatcat cgaagggccg ccctactata tacttcaagc
      301 attatggcgt atgacgatct acaggactat atggcggccc tcgaatcgaa gggcctgctc
      361 cggcgtgtcg gcgtcgaggt cgatcccgag ctcgaaataa ccgagatagc cgaccgcgtg
      421 atgaggcagc ccggcggtgg gccggccctg ctcttcgaga aggtcaaggg ctcgccctac
      481 ccgctcctga tcaacgctat gggctcggag gagcgcatgg cgatggcctt cggcgcctcc
      541 agcctcgacg agaaggccgc ggagatcgag gacctcatcg cgtgggcctg gaagcagcta
      601 cgcggcttca atatcctcaa ggccgtgccc gaggccctgc ccaagctacc cgtagccctg
      661 agcctcttcc cgaggcgggt gtcgcggccg aagtgccagg agctcgtctc cgacgaggag
      721 ggtttctcga gcctgccggt actcaagtgc tggcccctcg acggcggccg cttcctgact
      781 ctgccgctcg tatgcacccg cgacccggag accggcgtcc agaacgtcgg catgtaccgc
      841 atgcaggtct acgacgaccg tacggccggc atgcactggc acctccacaa ggacggcgcc
      901 cacttcttcc agaagtacca ggcccgcggc gagcgcatgc cggtcgcggt ggccctcggc
      961 tgcgaccccg cggtcaccta cgcgtcgacc gcccccctgc ccgagggcgt ctgggagatg
     1021 ctgttcgccg gcttcctgcg cggcaagagc gcggagataa ccaaggccac cctgagcggg
     1081 ctggacgtgc cggccgacgc cgagttcata ctcgagggct acgtcgaccc ctccgaggtc
     1141 cgcgtcgagg gccccttcgg cgaccacacg ggcttctact cgctcgagga cgagtacccg
     1201 gtcttccacc tggagcgcat aacgcgccgc aggtcgccgg tctatccggc gaccatcgtc
     1261 ggcataccgc ccaaggagga ctgctggatg gccaaggcga ccgagcggct cttcctgccg
     1321 ctcctcaagc agctctgccc ggagatcgtc gacctatcga tgccgctcga gggcgtgttc
     1381 cacaactgcg tcatcgtgtc gatcaagaag cgcttccccg gccaggcccg caaggtgatg
     1441 aacttcatgt ggggcatggg ccagatgatg tacacgaagc tcatcgtcgt ggtcgacgac
     1501 gacgtgaagc cgtccgacgt gtcgacggtc gcctggcgcg ccttcaataa tatcgacgcg
     1561 aagcgcgacc tcgtattctc cgacggcccc ctcgacgcgc tcgaccactc atcgccgcta
     1621 ccgcgctatg gcacgcggct cggcatcgac gcgacgcgca agggtcccga ggaagggcat
     1681 acgcggccgt ggccgtcgcc cctggccatg gacgagcata taaagagcct agtagacggc
     1741 cgctgggagc agtatggcct ctgagcccgg ctccgccgcg agccgccg
//
LOCUS       DAOOLN010000379         1788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_84463_length_1788_cov_2.814772, whole genome shotgun sequence.
ACCESSION   DAOOLN010000379 DAOOLN010000000
VERSION     DAOOLN010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84463_length_1788_cov_2.814772"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PLW80_05585"
     CDS             complement(<1..594)
                     /locus_tag="PLW80_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66011.1"
                     /translation="MSRFSRLFASIAASSLLCLPCLATLACSGGAPGASEARSSSRTP
                     MRAVAVSPEVDEPRVLAALSARGIDDALVASRVMVPFSDFSRVVDAPLDSALRRSRDA
                     DPRRTPFLEELASRFTATGPDGSAWTVVYLPGPTAERDEAAALSLTSLGADWAWDARS
                     GAHSSPILWLPAATFALWLVVRKPKSGRTARLVVAPVI"
     gene            complement(598..1077)
                     /locus_tag="PLW80_05590"
     CDS             complement(598..1077)
                     /locus_tag="PLW80_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HPB66012.1"
                     /translation="MEHQRVFFISALLRNNVFLAAALSLFMAQFAKALVAVFTNRKAG
                     IREIFSTLLWKTGGMPSSHSALVIAISTSIAFSSGIGSDVFALSFFFALVVIRDAMGV
                     RRTAGVQAKTLNSLGSRLSKRLHIPFKPVKEINGHTFPQVVAGNLIGFFIALAVNTL"
     gene            1171..>1788
                     /locus_tag="PLW80_05595"
     CDS             1171..>1788
                     /locus_tag="PLW80_05595"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPB66013.1"
                     /translation="MAYSTKRFIIGPDDSGRRADRVVRSLLSAMPLSAVYRLFRDGAV
                     RSGGRRIDGSSRLEAGATLDIRLPDTVEAPETSASREAAAADPRLACERAKRFSEIIL
                     LETPDLVVVNKPRGRLTHGPDGVDEDAKAYFADRMARSLAFAPGPLHRLDRNTSGALV
                     VSASQAGAAAFSEALRAGLVGKLYLALLGGELAGTERWIDRLSRDD"
BASE COUNT          323 a          573 c          620 g          272 t
ORIGIN      
        1 gatgaccggg gcgacgacga gcctggccgt ccttccgctc ttcggcttcc tgacgacgag
       61 ccacagcgcg aacgtcgcgg ccgggagcca gagtatcggg gacgagtgcg cgccggagcg
      121 ggcgtcccag gcccagtcgg ctccgagcga cgttagggac agagccgcgg cctcgtcgcg
      181 ctcggccgtc ggcccgggta ggtagacgac cgtccaggcc gagccgtcgg ggccggtcgc
      241 cgtgaacctc gaggccagct cctcgaggaa gggcgtacgg cgcgggtcgg cgtcgcggga
      301 gcggcgcagg gcgctgtcga gaggcgcgtc gacgacgcgg gagaagtcgc tgaaggggac
      361 catgaccctc gaggctacca gcgcgtcatc gatgcctcgg gccgagaggg cggccagtac
      421 gcgcggctca tcgacctcgg gcgagacggc cacggcgcgc atcggggtgc gcgacgacga
      481 gcgcgcctcg gaggccccgg gagcgccacc cgagcaggcg agcgtggcca ggcaaggcaa
      541 gcagagcagg ctggaggcgg ctatcgatgc gaagaggcgc gagaaccggc tcacgcgcta
      601 cagcgtgttg acggccaggg ctatgaagaa gcctatgagg tttccggcga cgacctgcgg
      661 gaaggtatgc ccattgatct ccttgaccgg cttgaagggg atatggaggc gtttcgatag
      721 cctggagccg agcgagttga gggttttagc ctgtacgcca gccgtgcgcc tgacgcccat
      781 cgcgtcgcgt atgaccacca gggcgaaaaa gaacgacagc gcgaacacat ccgagcctat
      841 tccgcttgaa aatgcaatcg aggtggatat ggctatgacc agggcgctgt ggctcgaggg
      901 cataccgccg gtcttccata ggagcgtaga aaatatctcg cggataccgg ccttgcggtt
      961 cgtgaagacc gcgacgagag ccttggcgaa ttgcgccatg aagaggctta acgccgccgc
     1021 caggaacacg ttattcctga gtaaagcgct tataaaaaat actcgctggt gttccatcgc
     1081 gagaatttca agtataatcg cttctcttgt caagcctata cgagtgtcaa gcctattcga
     1141 gccgtttcac gggagtcgct gagaacaacc atggcgtaca gcaccaagcg ctttataatc
     1201 ggcccggacg attcgggcag gcgcgccgac cgcgtcgtgc gctcgctcct gtcggcgatg
     1261 ccgctctcgg ccgtataccg cctgttccgc gacggcgccg tccgatccgg aggccgtcgc
     1321 atcgacggct cgtccaggct cgaggcgggc gcgaccctcg atatccgcct gcccgatacc
     1381 gtagaggcgc cggaaacgag cgcgtcgcgg gaagcggccg ccgccgatcc gcggctcgcc
     1441 tgcgagcggg ccaagcgctt ttcggagatc atactgctcg agacgccgga cctcgtcgtc
     1501 gtcaataagc cgcggggacg cttgacccac ggaccggacg gcgtcgacga ggacgcaaag
     1561 gcctatttcg cggaccgcat ggcccggtcc ctcgccttcg cgccgggccc tctccacagg
     1621 ctcgaccgca acacctccgg agccttggtc gtatcggcca gccaggccgg cgccgcggcc
     1681 ttcagcgagg cgctccgggc cggcctggta ggcaagctat acctcgcctt gctcggcggc
     1741 gagctggctg gcacggagcg ctggatcgac cgcctgagcc gcgacgac
//
LOCUS       DAOOLN010000380         1785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_84671_length_1785_cov_3.124855, whole genome shotgun sequence.
ACCESSION   DAOOLN010000380 DAOOLN010000000
VERSION     DAOOLN010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84671_length_1785_cov_3.124855"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PLW80_05600"
     CDS             <1..728
                     /locus_tag="PLW80_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66014.1"
                     /translation="RPGVLAGARAATTAAPPKGRSSMRRGVSVALAAVVAVVASSSSC
                     GRGPAPATRPSLAASIKVARGNRLFAEGFYHEAAALYLEAGAGSDPTASYDLANVFSA
                     LGEGEAASSMRARAVALGEADGYSGLQASAWFNEGVDAYAAGAYDEAAAAFRKALAEA
                     SSVGASVGAFGLGPVARVELSRAYELALAALADKRESSASERGAYEAAQVPGESRPLS
                     LSRIDERTLFAPGSSESAGGEDH"
     gene            725..>1785
                     /locus_tag="PLW80_05605"
     CDS             725..>1785
                     /locus_tag="PLW80_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66015.1"
                     /translation="MMRASMRAAAALLCLLAGAAGWAQDERRVAISPDRPAVGSSFVI
                     EAFLPGERAEALEAIEPDILGPARFSGADVRPAQTSGGGPAATITYRFVVEGAGRIDV
                     LGLIAVSGTRTLSLGSWSIETESLDRGPVARIGSWSAPASVYEREVFVIEARGPDGSP
                     VACPALAVEGLLLDPIPGRTGAYYAVAPAAGAARLPSLRLDDGYGPFELGERPVRMRS
                     LPAEAEGVMAVGGPWRLELSFPGQAGSADKLSFSSTSLGSGDTLSWELRAVGSGWPGL
                     AGWPRLTIRAEGIGERRVAEAVRYVERGKGRLDYVAGARGAFAAAEPGVYVIEPEPYA
                     WFDPVAQAVRYAAASAVRVV"
BASE COUNT          210 a          603 c          690 g          282 t
ORIGIN      
        1 gccgccctgg cgtcctggcc ggcgctcgag ccgcgacgac cgccgctccg ccgaaaggac
       61 ggtcgtccat gaggcgcggc gtcagtgtcg cgctagcggc cgtcgtcgcc gtggtagcct
      121 cgtcgtcgtc ctgcggtcga ggcccggccc ccgcgacgcg gccgtctctg gcagcgtcga
      181 tcaaggtggc gcggggcaat aggctattcg ccgagggctt ctaccatgag gccgcggccc
      241 tgtacctgga ggccggggca ggcagcgatc cgacggcctc gtacgacctg gccaacgtat
      301 tctcggccct cggcgagggc gaggcggcct cgtcgatgcg ggcgcgggcg gttgcgctcg
      361 gcgaggccga cggttactcc gggctacagg ccagcgcctg gttcaacgag ggcgtcgacg
      421 cctacgccgc tggcgcctat gacgaggcgg cggcggcttt caggaaggcg ctggccgagg
      481 cgtcctccgt cggagcctct gtcggcgcct tcggcctcgg gccggtcgcg cgggtcgaac
      541 tatcgcgggc ctacgaactg gccctcgcgg ccctggccga caagcgcgag tcgagcgcat
      601 ccgagcgcgg cgcctacgag gcggcgcagg taccgggcga atcgcggcct ctgtccctgt
      661 ccaggatcga cgagcgtact ctgttcgccc cgggctcctc cgagtccgcc ggcggggagg
      721 accattgatg cgcgcttcga tgcgggccgc ggcggctttg ctgtgcctgc tcgccggcgc
      781 ggccgggtgg gcccaggacg agcgtcgtgt ggccatatcg ccggatcggc cggccgtcgg
      841 ctcgtccttc gtcatcgagg cgtttctgcc gggcgagcgc gccgaggcgc tcgaggcgat
      901 cgaaccggat attctggggc cggccaggtt ctccggggcg gacgtacggc cggcgcagac
      961 gtccggcggc gggccggccg cgactattac gtatcgcttc gtggtcgaag gcgcaggccg
     1021 gatcgacgtg ctgggcctga tcgcggtatc ggggacccgt accctgagcc tcgggtcttg
     1081 gtcgatcgag accgagagcc tcgatcgggg gccggtcgcc cgaatcggtt cgtggtcagc
     1141 ccccgcttcc gtctacgagc gagaggtgtt cgtgatcgag gccaggggcc ccgacggctc
     1201 tccggtagcc tgtccggcgc tggccgtgga gggactgctg ctcgatccga tacccggccg
     1261 gacgggcgcc tactacgcgg tcgctccggc agccggggcc gctcggttgc cttcgctcag
     1321 gctcgatgac ggctacggcc ccttcgagct cggtgagcgt cccgtgagga tgcgttcgtt
     1381 gccggccgag gccgagggcg tcatggccgt cggcgggccg tggcggctgg agctgtcgtt
     1441 tccggggcag gccggctcgg ccgacaagct gagcttcagc tcgacgagcc taggctcggg
     1501 agatacgctg tcctgggagc tacgcgccgt aggctcgggc tggcccggcc tcgccggctg
     1561 gccgaggctg acgatacggg cggaggggat cggagagcgt cgcgtcgcgg aggcggtacg
     1621 gtacgtcgaa cgcgggaagg ggcgcctcga ctacgtggcc ggcgcgcgtg gcgccttcgc
     1681 ggcagccgag ccaggcgtct acgtgatcga gccggagccg tacgcctggt tcgaccccgt
     1741 cgcccaagcc gtacgatacg ccgccgcgtc ggctgtcagg gtggt
//
LOCUS       DAOOLN010000381         1783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_84785_length_1783_cov_3.279514, whole genome shotgun sequence.
ACCESSION   DAOOLN010000381 DAOOLN010000000
VERSION     DAOOLN010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84785_length_1783_cov_3.279514"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PLW80_05610"
     CDS             <1..672
                     /locus_tag="PLW80_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPB66016.1"
                     /translation="GFSIPINNAKKVIDDIISGGSVRYGWLGVLLSDAPKATLTELAL
                     GSTKGAFIGHVFTDGPAAKGGIKPGDYVVEIDGKTVSSMDQLVRIVGDLPVGSKSSFA
                     VIRDGKRLQLRVTIDERKEASAADYSKLWPGLEIAPIDAAAREELGLDKRSGGALVTS
                     VIAKSPASTLGLKAGDVVTEVNGVAIGSVADYYRVLNDPKTQKFQFTVLRDGQTLTTL
                     ALIRK"
     gene            703..1308
                     /locus_tag="PLW80_05615"
     CDS             703..1308
                     /locus_tag="PLW80_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HPB66017.1"
                     /translation="MSKEFISYNQVRNNALKLAHRIHLDGFVPDIIYVSLRGGAYLGN
                     VMSEYFKAVRSDARPVFYAAVVARSYTDIHKNEQVRVDGWTYSPEYLRNGDKVLLVDD
                     IYDTGRTINHLASVILEKGVPRRDLKIAVHDYKIVDYRGEQPAIKPDYWCRLHEIRKP
                     EDERWIHYMSHELIGLTEEELKEYYYPEDPELQEILSVLKG"
     gene            1308..>1783
                     /locus_tag="PLW80_05620"
     CDS             1308..>1783
                     /locus_tag="PLW80_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66018.1"
                     /translation="MSAAGLRSLTAVALFAAAALCHADPDTAASYEPAPGVLGPSPGA
                     YAATQVLVPATLSAGARAAYRFSGQEAWLPFDRPLYLSAFPGEERHYSIEFGNPRSPS
                     PTTLSYAIDLRPPEPPTVQPQPPAPPPTVTPPVEPPVVVLPPQVTQLAVVRYNWTP"
BASE COUNT          346 a          619 c          527 g          291 t
ORIGIN      
        1 ggattctcca tcccgatcaa caatgccaag aaggtgatag acgatattat ctcgggcggt
       61 tccgtccgtt acggttggct cggcgtactc ctgtcggacg cgccaaaggc gacgctaacg
      121 gagctggccc tcggctcgac gaaaggcgcc ttcatcggcc atgtgttcac tgacggcccc
      181 gcggccaagg gcggcatcaa accgggcgac tacgtcgtcg agatcgacgg caaaaccgtc
      241 tcctcgatgg atcagctcgt gcgcatcgtc ggcgatctgc cggtcggctc caagtcgtcc
      301 ttcgcggtga tccgcgacgg caagcggctc cagctcaggg tgacgataga cgagcgcaag
      361 gaagcgagcg cggccgacta ctcgaagcta tggcccggcc tcgagatagc gccgatcgat
      421 gccgccgcca gggaggagct cggcctcgat aagcgttccg gcggcgcgct cgtcacgagc
      481 gtcatcgcca agagccccgc gtcgactctc ggcctcaagg ccggcgacgt cgtcaccgag
      541 gtgaacggag tcgcgatcgg ctccgtcgcc gactattacc gcgtgctcaa cgaccctaag
      601 acccagaagt tccagttcac cgtgctgcgc gatggccaga cgctgaccac gcttgccttg
      661 atacgcaagt agtgctacag taaagaacaa agagaggttc cgatgagcaa ggaattcata
      721 tcgtacaacc aggtgcggaa caacgccctc aagctggcgc ataggataca cctggacggg
      781 ttcgtccccg acatcatcta cgtttccttg cgcggcggag cctatctcgg caacgtcatg
      841 agcgaatact tcaaggcggt caggagcgac gcgcggccgg tgttctacgc ggccgtcgtc
      901 gcccgctcct ataccgacat ccataagaac gagcaggtcc gcgtcgacgg ctggacctat
      961 agccccgagt acctgaggaa cggcgacaag gtgctgctcg tcgacgacat ctacgatacc
     1021 ggtcggacca tcaaccacct ggcgagcgtc atactcgaaa agggcgtacc ccgccgcgac
     1081 ctcaagatcg ccgtgcacga ctacaagata gtcgactacc gcggcgagca gccggccatc
     1141 aagccagact actggtgccg gctccatgag atacgcaagc ccgaggatga gcgctggata
     1201 cactacatga gccacgagct gatcgggctg accgaagagg aactcaagga atactactat
     1261 cccgaggatc cggagctcca ggagatcctc tcggtactga agggctgatg tcggccgccg
     1321 ggcttcgatc gctaacggct gtagcgctgt tcgccgcggc cgccctatgc cacgccgacc
     1381 ccgatacggc cgcctcctac gagccggctc ccggggtgct cggtccgagc cccggcgcct
     1441 acgcggctac acaggtcctc gtaccggcga ccctatcggc cggcgcccgc gccgcgtacc
     1501 gcttttccgg tcaggaggcg tggttgccgt tcgaccggcc gctctacctc tcggccttcc
     1561 cgggcgagga gcgccactac tcgatagagt tcggcaaccc acgttccccc tcccccacga
     1621 ccttgtcgta tgccatagac ctgcgcccgc ccgagccgcc gaccgtgcag ccccagccgc
     1681 ctgccccacc gcccaccgtc accccgccgg tcgagccgcc cgtcgttgtc ctgcccccgc
     1741 aggtcacgca actggcggtc gtgcgctaca actggacgcc caa
//
LOCUS       DAOOLN010000382         1779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_85070_length_1779_cov_3.256961, whole genome shotgun sequence.
ACCESSION   DAOOLN010000382 DAOOLN010000000
VERSION     DAOOLN010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85070_length_1779_cov_3.256961"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1779
                     /locus_tag="PLW80_05625"
     CDS             <1..>1779
                     /locus_tag="PLW80_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HPB66019.1"
                     /translation="VIRDKIIADTGDAKVDIGLTATTGSSREILGVFLETPGVYNEII
                     AHAVGTAYFDKGVETIFEIGGQDAKYVLLKNGVPIDYAMNEACSAGTGSFLEESSSGD
                     LSIHSAKDIAPIALAADAPLKFGEHCSAFINSDIRKAVQQGATRENITAGIVVSIVAN
                     YLNRVVGNRTIGGKVFLQGGVAKNEAVPLAFAMMLDKEILVPPSPELMGCFGVALLAK
                     RKRAEGLLDDTVVDLEAAISRDIVYERVFTCKSCENNCPIQVLKVNGHPYMFGGRCNK
                     YTNTRKALKDVPVFDYVEKRQKLLYETFAAPADAIVARRDYTVGIPRAFSTHSLYPLY
                     SWFFHELGIKTVLSTEVAQQGVVRVESAYCFPAEIAHGAVQDCLDKGVDYVLLPHFRD
                     MASYEDDVHANTCPITQSLPYYIAKAFPEIDEQKFLPLVVSFKFGDKKALELFCTMTA
                     RLGIGEAETKAAFDKALAKQREYFAAAAALGREALEEAKAAERPVIAVLGRPYNAFTP
                     EANMGIPRKFTTRGYSVIPFDILPLEETDNRFPNMYWYYGQQDMRAAQVLKDNPNIYV
                     TWITNFSCAPDSFMLHYLKWTMGQKP"
BASE COUNT          370 a          627 c          497 g          285 t
ORIGIN      
        1 ccgtcatccg cgacaagatc atcgccgata cgggcgacgc caaggtcgac atcggactga
       61 ccgccacgac tggaagctcc cgcgagatac tcggcgtgtt cctcgagacg cccggcgtgt
      121 acaacgagat catcgcccac gccgtcggca ccgcctactt cgacaaaggt gtggagacca
      181 tcttcgagat cggaggccag gacgccaagt atgtgctgct caagaacggc gtaccgatcg
      241 actacgcgat gaacgaggcc tgttcggcgg gcaccggtag cttcctcgag gagtcgtcgt
      301 ccggcgacct ctccatccac agcgccaagg acatcgcgcc gatcgcgctg gccgcggacg
      361 cgccgctcaa gttcggcgag cactgctcgg ccttcatcaa ctcggacatc cgcaaggccg
      421 ttcagcaggg agcgacgcga gagaatatca ccgccggcat cgtcgtatcc atcgtcgcca
      481 attacctgaa ccgcgtcgtc ggcaaccgca cgataggcgg caaggtcttc ctgcagggcg
      541 gcgtggccaa gaacgaagcc gtaccgctag ccttcgcgat gatgctcgac aaggagatcc
      601 tcgtcccgcc gtcgcccgag ctcatgggct gcttcggcgt cgcgctgctc gccaagcgca
      661 agcgcgccga ggggctcctc gacgatacgg tcgtcgacct cgaggccgcg atcagccgag
      721 acatcgtcta tgaacgcgtc ttcacctgca agtcctgcga gaacaactgc ccgatccagg
      781 tcctcaaggt caacgggcac ccctatatgt tcggcggacg atgcaacaag tacacgaaca
      841 cccgcaaggc gctcaaagac gtaccggtct tcgactacgt cgagaaaagg cagaagctac
      901 tctacgagac cttcgcggca cccgccgacg ccatcgtcgc caggcgcgac tacacagtcg
      961 gcatcccgcg cgccttctcg acgcacagcc tctatcccct ctactcctgg ttcttccacg
     1021 agctcggcat caaaaccgta ctgtccaccg aagtcgccca gcagggagtc gtccgcgtcg
     1081 aaagcgcgta ctgcttcccg gcggagatcg cccacggcgc ggtgcaggac tgcctggaca
     1141 agggcgtcga ctacgtgctc ctgccccact tccgcgacat ggcctcctac gaggacgacg
     1201 tgcacgcgaa cacctgcccc atcacgcaga gcctcccgta ctatatagca aaggccttcc
     1261 ccgagatcga cgagcagaag ttcctaccgc tcgtcgtcag cttcaagttc ggcgacaaga
     1321 aggcgctcga actattctgc accatgacgg cgcgcctggg catcggcgag gccgagacga
     1381 aggccgcctt cgataaggcg ctcgccaagc agcgcgaata cttcgccgcg gccgccgccc
     1441 ttggacgcga ggccctcgag gaggccaagg ccgcggagcg cccggtcatc gccgtactcg
     1501 gccgcccgta caacgcgttc acccccgaag cgaacatggg tattccgagg aagttcacca
     1561 cacgcggcta ctccgtcata cccttcgata tcctcccgct cgaggagacc gataaccgct
     1621 tcccgaacat gtactggtac tacggccagc aggacatgcg cgccgcgcag gtactcaagg
     1681 acaacccgaa catctacgtg acctggatca cgaatttctc ctgcgcgccc gactccttca
     1741 tgctgcacta cctcaaatgg accatgggcc agaagccct
//
LOCUS       DAOOLN010000383         1777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_85219_length_1777_cov_2.930894, whole genome shotgun sequence.
ACCESSION   DAOOLN010000383 DAOOLN010000000
VERSION     DAOOLN010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85219_length_1777_cov_2.930894"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLW80_05630"
     CDS             <1..227
                     /locus_tag="PLW80_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HPB66020.1"
                     /translation="GLGLRPELYMFGLLIGSCLGGNLTPFGASANVVAVSMLKKRGTV
                     VRFSEWIKVAGPFTVLTTLASSLFVWLVWS"
     gene            complement(375..830)
                     /locus_tag="PLW80_05635"
     CDS             complement(375..830)
                     /locus_tag="PLW80_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPB66021.1"
                     /translation="MVTFEAKDRNVEILEVVVEKAAKKSIEEANVLISGGRGLGNLDN
                     FNKLQGIADELGGLVSASRAVVDAGWVGQDRQVGQTGKTVRPDVYFACGISGMIQHVA
                     GMEDSDFIVAINKNSSAPIFEYADVGIVADINKVVPALVEELKAAKAAR"
     gene            complement(906..1388)
                     /locus_tag="PLW80_05640"
     CDS             complement(906..1388)
                     /locus_tag="PLW80_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66022.1"
                     /translation="MNISDYRGVMVFAEQRRGIVQKIAYELLGVGRRIADDLEEPLLA
                     LLVGHGLPDSAAAELIAQGADTVVVVDDPALDVYMTEPYAKAMTAAIERYKPGIVFTG
                     ATTIGRDLAPRVSARVRTGLTADCTGLEIEDGTRNLLMTRPAFGGNIMATIVCPEHRP
                     "
     gene            complement(1414..>1777)
                     /locus_tag="PLW80_05645"
     CDS             complement(1414..>1777)
                     /locus_tag="PLW80_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HPB66023.1"
                     /translation="VSYVSELEATEGSVVVKRVFEDGYQRIKVKLPCLLTALKDMNRP
                     RYMSVSGIFDAYRREVKVWGLADIEVDQDNIGLKGSPTRVKKSFTKGVKSAGKVYEVD
                     AKEGARIIVEALKEKYVI"
BASE COUNT          284 a          603 c          547 g          343 t
ORIGIN      
        1 ccgggctcgg cctgcgcccg gaactgtaca tgttcggcct gctcataggc tcgtgcctcg
       61 gcggcaacct gacgccgttc ggcgcttcgg ctaacgtcgt cgccgtgagc atgctcaaga
      121 aacgcggcac cgtggtacgc ttcagcgagt ggatcaaggt agccggaccg ttcaccgtcc
      181 tgactaccct ggcttcgagc ctgttcgtct ggctcgtctg gagctaggcg ccgtccgcca
      241 gcggaacgcg aagcgcaagg cgacgaacct gtccaggcct cgattaaaaa gcgcgcggcc
      301 ggatcgatcg atccatacga aaaaggccgc cccggtgggg cggcccaatc tagacgatag
      361 gtcgctctag cctatcaccg tgccgccttg gcggccttga gctcctcgac gagggccggc
      421 accaccttgt tgatgtcggc gacgatgccg acatcggcgt actcgaagat cggcgccgac
      481 gagttcttat tgatcgcgac gatgaagtcc gagtcctcca tgccggcgac gtgctggatc
      541 atgccggaga tgccgcaggc gaagtacacg tccggccgaa ccgtcttgcc ggtctggccg
      601 acctgccggt cctgccccac ccagcccgcg tcgaccaccg cgcgcgacgc cgacacgagg
      661 ccgccgagct cgtcggctat gccttgcagc ttgttgaagt tgtccaggtt cccgaggcct
      721 cggccgccgg agatgagcac gttcgcttcc tcgatgctct tcttggccgc cttctcgacg
      781 accacctcga ggatctccac gttccggtcc ttcgcctcga acgtgaccac cagcctctcg
      841 actgcgccct ttcgggaatc atcgcgctcg aggcggcgca tcacgccggg ccgcacggtc
      901 gacatctacg gccggtgttc cgggcagacg atcgtagcca tgatgttgcc gccgaaggcg
      961 ggccgcgtca tcaagaggtt acgcgtaccg tcctctatct cgaggccggt gcagtcggcg
     1021 gtcaggccag tcctgacgcg ggcggacacg cgcggcgcca ggtcgcggcc tatcgtcgtg
     1081 gcgccagtga agacgatgcc gggcttgtag cgctcgatcg ccgcggtcat cgccttggcg
     1141 tagggctcgg tcatgtagac gtccagggcc ggatcgtcca cgacgacgac cgtgtcggcg
     1201 ccctgggcta tcagctcggc cgccgcgcta tcgggcaggc cgtggccgac gagcagggcc
     1261 aggagcggct cctcgaggtc gtcggctatg cgcctgccca cgccgaggag ctcgtaggct
     1321 atcttctgca cgatccctcg ccgctgttcg gcgaagacca tcacgcccct ataatcggat
     1381 atgttcattg cgtttctcct gtcgtacgcc gccttagatg acgtacttct ccttgagcgc
     1441 ctcgacgatg atccttgcgc cttctttggc gtcgacctcg tagaccttgc cggccgattt
     1501 gacgcccttc gtgaaagact tcttgaccct ggtcggcgag cccttgagtc cgatattgtc
     1561 ctgatcgacc tcgatatcgg cgaggcccca gaccttgacc tcgcgcctat acgcgtcgaa
     1621 gatgccggag accgacatat agcgcggccg gttcatgtcc ttgagcgccg tcaggaggca
     1681 gggcagcttg accttgatac gctggtaacc gtcctcgaag acccgcttga ccacgaccga
     1741 gccttccgta gcctcgagct cgctgacgta gctgacg
//
LOCUS       DAOOLN010000384         1775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_85373_length_1775_cov_2.595349, whole genome shotgun sequence.
ACCESSION   DAOOLN010000384 DAOOLN010000000
VERSION     DAOOLN010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85373_length_1775_cov_2.595349"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PLW80_05650"
     CDS             <1..200
                     /locus_tag="PLW80_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HPB66024.1"
                     /translation="AVYFAAIGGAGALLARGIKEAEVVAFAELGPEAIYRLRVEDFPV
                     TVAVDSTRADLYAIGREPYRR"
     gene            218..1462
                     /locus_tag="PLW80_05655"
     CDS             218..1462
                     /locus_tag="PLW80_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HPB66025.1"
                     /translation="MSLRDDALKLHRDHAGKLEIRSTVPLRDKADLSLAYTPGVAEPC
                     LEIAARPDAVYEYCGKGNLVAVVSDGSAVLGLGDIGPAAAIPVMEGKAALFKAFGGVD
                     AFPICLGTKDVDELVDTIARLEPVFGGVNLEDISAPRCFEVEARLKERMAIPVFHDDQ
                     HGTAIVLLAGLLNALKLVGKTMGELVVAINGAGSAGIAIARMLLAAGAGDVILSDIRG
                     ILRDGDESGLNRAQAEIAALTNRDKRRGLLADAMRGSDVFIGVSRANLVTRDMVRAMA
                     RDPIVFAMANPVPEIDPDEALAAGARVVGTGRSDRPNQVNNVLASPGVFRGALDARAS
                     DIDEAMKLAAAYAIAGVVPDGELREDYILPTPFDPRVAPAVARAVAKAAIDAGLARVA
                     ADPDAIAERMAAYLARTRSSGS"
     gene            complement(1451..>1775)
                     /locus_tag="PLW80_05660"
     CDS             complement(1451..>1775)
                     /locus_tag="PLW80_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HPB66026.1"
                     /translation="SFGLVYPLYVRKAEKKGRTKDEVDEVIAWLTGYAKQGIEAIAAS
                     DTDLEHFFGSAPRMNPNASKITGVICGHRVEEIEDDILRKVRYLDKLIDELAKGRPME
                     KVLRA"
BASE COUNT          287 a          624 c          587 g          277 t
ORIGIN      
        1 gcgcggtcta cttcgccgcg ataggcggcg ccggcgccct gctcgccagg gggatcaagg
       61 aggccgaggt cgtcgccttc gccgagctcg ggccggaggc gatctacagg ctgcgcgtcg
      121 aggatttccc ggtcactgtc gccgtcgatt ccacgcgggc ggacttatat gctataggta
      181 gggagccgta tcggcgatag acctacgagg agtacccatg tccctacgcg acgacgcgct
      241 caagctacac cgcgaccacg ccggcaagct cgagatacgc agcacggtgc ccctgaggga
      301 caaggccgac ctcagcctgg cctatacgcc cggcgtcgcc gagccctgcc tcgagatagc
      361 cgccaggccg gacgccgtct acgagtactg cggcaagggc aacctggtcg cggtcgtgtc
      421 ggacggcagc gcggtgctcg gcctcggcga tataggcccc gccgccgcga taccggtaat
      481 ggagggcaag gccgccctct tcaaggcctt cggcggcgtc gacgcgttcc ccatctgcct
      541 gggcacgaag gacgtcgacg agctggtcga taccatagcc cgcctcgagc cggtcttcgg
      601 cggcgtcaac ctcgaggaca tctccgcgcc gcgctgcttc gaggtcgagg ccaggctcaa
      661 ggagcgcatg gccatcccgg tgttccacga cgaccagcac ggcactgcga tcgtcctcct
      721 ggccgggctc ctgaacgcgc tcaagctggt aggcaagacg atgggcgagc tcgtcgtggc
      781 catcaacgga gccggctcgg cgggcatagc gatagccagg atgctgctgg cggccggggc
      841 gggcgacgtt atcctgtccg acatacgcgg catactccgc gacggcgacg agtcggggct
      901 gaaccgggcc caggccgaga tcgcggcgct gacgaaccgg gataagcgac gaggcctcct
      961 ggccgacgcg atgcgcggca gcgacgtgtt cataggcgtc tccagggcca acctggtgac
     1021 ccgagacatg gtccgcgcca tggctcgcga cccgatcgtg ttcgcgatgg ccaatcccgt
     1081 acccgagata gaccccgacg aggccctggc ggccggagcc cgagtcgtcg gcaccgggcg
     1141 ctccgaccgg cccaaccagg tgaacaacgt gctcgcttcc cccggcgtct tccgcggcgc
     1201 cctcgatgcg cgcgcctccg acatcgacga ggcgatgaag ctcgcggccg cctacgccat
     1261 cgccggcgtc gtgcccgacg gcgagctcag ggaagactac atcctgccga ccccattcga
     1321 cccgcgcgtc gcgcccgccg tggcgcgcgc cgtcgcgaag gccgcgatcg acgcgggcct
     1381 ggccagggta gcggccgacc ccgacgcgat agccgagcgc atggcggcct acctggcgag
     1441 gacgaggagc tcaggctcgt agcactttct ccataggcct acccttggcc agctcgtcga
     1501 tcagcttgtc gaggtacctg accttgcgca ggatatcgtc ctctatctcc tcgacgcggt
     1561 ggccgcagat gacgccggtg atcttcgacg cgttcgggtt catcctcggc gcggagccga
     1621 agaaatgctc caagtcggta tcgctcgccg cgatcgcctc gatgccctgc ttggcgtagc
     1681 ccgtcagcca cgcgatgacc tcgtcgacct cgtccttcgt ccgtcccttc ttctccgctt
     1741 ttcggacgta gagaggatag acgagcccga agctc
//
LOCUS       DAOOLN010000385         1773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_85533_length_1773_cov_2.817811, whole genome shotgun sequence.
ACCESSION   DAOOLN010000385 DAOOLN010000000
VERSION     DAOOLN010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85533_length_1773_cov_2.817811"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PLW80_05665"
     CDS             complement(<1..562)
                     /locus_tag="PLW80_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPB66027.1"
                     /translation="MVAPLLVASGYSFHYGCATPAELAGRAKALGYEALALADRDGLY
                     GVPAFIESCEAEDLKPLIGAELSLDGGGPGSGRALAIAMDRAGFSRLCRLLSARAESG
                     FDARAELLRDSVGLAVASDDDRLLGSPGSDGFYALLSPCRRGSWRRLLASGRRPLATG
                     EVRFIEPGDRALQRLLVAIGSGRTVYE"
     gene            complement(556..>1773)
                     /locus_tag="PLW80_05670"
     CDS             complement(556..>1773)
                     /locus_tag="PLW80_05670"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66028.1"
                     /translation="SACCELSAMPSGRVLHINAVGLMAELEEALEPSLRRRPFVVANE
                     RLARAVVLDVSALAHREGARPGMSLAAARALLPGLAARPPRSELYRDAEDRLWRLALE
                     LTPLVERAGRGHLFADLTGTGRLNGSPDDAAQRLRSRILADTGLSPALALSSTKTASK
                     VATRVFRPGGFVALSTAEEGPLVRRQPASLLPGVGPALLRRLALLDIDDIGGIADLDY
                     TRAAAVGPRGRELVARARCVDDSPVDPRPLGSRSASAEAVLEPDTADPETLRLRLLSL
                     SAELAFSLRREGLGFSGASVSLGYTDGLRASASACSGRRLWRDDEALALALEALGRAR
                     TRRVRVRSVALTLSRLGAASPELDLFEPGESRRSRLQSALDAIRLRYGSGAIEPCALA
                     ELGAPGPSGAIRW"
BASE COUNT          267 a          643 c          680 g          183 t
ORIGIN      
        1 cctcgtagac cgtccgcccc gagcctatcg cgacgagcag gcgctggagg gcccggtccc
       61 ccggctcgat gaagcggacc tctccggtcg cgagcggccg ccggccggag gcgagaaggc
      121 ggcgccagga gccgcgccgg cacggcgaca ggagcgcgta gaagccgtcc gagcctggcg
      181 agccgaggag ccgatcgtcg tcggaggcca ccgcgaggcc gacggagtcg cggagcaatt
      241 ccgcgcgagc gtcgaagccg gattcggccc gggcgctgag gaggcggcag agacgcgaga
      301 agcccgctcg gtccatcgct atcgcgaggg cccggccgct tccgggacca ccgccgtcga
      361 gcgacagctc cgcgccgatg agcggcttga ggtcctcggc ctcgcaggat tcgatgaagg
      421 ccggtacgcc gtagagcccg tcgcggtcag caagagcgag cgcctcgtaa ccgagggcct
      481 tggcgcgccc ggccagctcc gcgggcgtcg cgcagccata atggaaggaa tagcccgagg
      541 cgaccaggag cggcgctacc atcggatcgc cccggacggg cctggcgcgc cgagctcggc
      601 cagggcgcag ggctcgatgg cgccggaacc gtaacgaagg cgtatcgcgt cgagcgccga
      661 ttgcagcctc gagcggcgcg actcgcccgg ctcgaacagg tccagctccg ggctagcggc
      721 gccgaggcga gatagcgtca gcgcgaccga gcgtacgcga accctcctgg tcctggcgcg
      781 gccgagcgct tcgagggcca gggcgagggc ctcatcgtcc cgccacagcc ggcggcccga
      841 gcaggccgag gccgaagcgc gaagcccgtc ggtatacccg agcgacaccg aggcgcccga
      901 gaacccgagc ccctcgcgcc ggagcgagaa agcgagctcc gccgacaggg acaggagcct
      961 gagcctcagc gtttccgggt cggccgtgtc gggctcgagc acggcctcgg ccgacgcgct
     1021 ccggctcccg aggggccgcg ggtcgacggg cgagtcgtcg acgcaacggg cgcgggccac
     1081 gagttcccgg cccctcgggc cgacggcggc cgcccgcgtg tagtccaggt cggcgatacc
     1141 gcctatgtcg tcgatgtcga gcagcgccag cctgcgcagg agggccggac cgacgcccgg
     1201 cagcagggac gccggctggc gcctgacgag gggcccttcc tccgcggtcg acagggcgac
     1261 gaagccgccg ggcctgaaga cccgggtcgc caccttgctc gccgtcttgg tggacgacag
     1321 cgccagggcc gggcttaggc cggtgtcggc gagtatgcgc gagcgcagcc gctgcgcggc
     1381 gtcgtccggc gagccattca ggcggcccgt gcccgtcagg tcggcgaaga ggtgtcccct
     1441 ccccgcccgc tcgacgagcg gcgttagctc gagcgccagg cgccagagcc ggtcctccgc
     1501 gtcccggtac agctcggagc gcggcggcct cgcggccagg ccgggcagga gcgcgcgggc
     1561 cgcggccagg ctcatgccgg gccgagcgcc ctcccggtgc gccagggcgg acacgtcgag
     1621 cacgacggcc cgcgccaggc gctcgttggc cacgacgaag gggcgacggc ggagcgacgg
     1681 ctcgagcgcc tcctcgagct cggccatcag cccgacggcg ttgatatgca ggacgcgacc
     1741 cgacggcatg gccgacagct cacagcaggc gga
//
LOCUS       DAOOLN010000386         1771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_85679_length_1771_cov_3.155594, whole genome shotgun sequence.
ACCESSION   DAOOLN010000386 DAOOLN010000000
VERSION     DAOOLN010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85679_length_1771_cov_3.155594"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..386)
                     /locus_tag="PLW80_05675"
     CDS             complement(159..386)
                     /locus_tag="PLW80_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HPB66029.1"
                     /translation="MAVSVYSTPSCSYCRLVKDWLKARNVRFDDYNVAQDQRRAEEMV
                     RKSGQMGVPVTDVNGRVIVGFNTAELERALR"
     gene            483..>1771
                     /locus_tag="PLW80_05680"
     CDS             483..>1771
                     /locus_tag="PLW80_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66030.1"
                     /translation="MSHTGHGGLDPLKAGGLAPIAIRAAIAAAWLLSAGLDAWPSPQR
                     AASAIGALELSNVDGLDCKAAIATAAAGLRFSYRVPVPASDDPAEVGFTMTLPYLLVG
                     SARPVGLAAFVEAPASAIVTLLGRSGAPLEAAWPLAATRVGAALGDDYGAYAELPAFD
                     GGSAGDWAAYGFWAAPRGSPLSALASVVETEAEPGGLGWYDPPAPAGRRAYGALGVSV
                     GGPAWAAAGAAAASLGLPGRDAAAGRAEARLSVAGYRLELVASCADRFWVDVDGGQAP
                     FLRADLSLRRSRSGLAWALGWRYLRPDAYTPEGTAVSMRASCDLAAWLGLASVRARLS
                     LAGSAEAGSAEAGSAEAGSSVVELDARYRPAPAPWLSLRSSWRVVGGDSERFDLGAAI
                     SAGSRWPLELESGLRFVPEGRLYKAGLSLGVSGDRASV"
BASE COUNT          229 a          629 c          621 g          292 t
ORIGIN      
        1 tacagataga cgagtacggc aacgtggtcg agatcgaggt ggacgcggac ggcaatccca
       61 tcgtcgaccc ggatgatgac gacgacgatg acgacgactc ggatgacgaa gactagcggg
      121 agccgtttca gccggccagg ctccccacgg gccgacgtct agcgcagcgc gcgctccagt
      181 tcggccgtat tgaatccgac gatgacgcgt ccgttgacgt ccgtcaccgg cacgcccatc
      241 tgtccggatt tcctgaccat ctcctcggcc cgcctctgat cctgggccac gttgtaatcg
      301 tcgaagcgta cgttcctggc cttgagccag tcctttacca gcctgcagta cgagcagctc
      361 ggcgtcgagt ataccgatac agccatatag cgcctcctaa tatatggaaa agtatatgta
      421 tagaataccg atagatacgc cggtcgtcaa gcgaggcggc tccccgcgtc gtatgcgccc
      481 gtatgagtca tacaggacac ggcggccttg atccgctcaa agccggaggg ctagctccga
      541 tcgcgatcag ggccgcgatc gccgcggctt ggctcctctc ggccggcctt gacgcatggc
      601 cctctcctca gcgggccgcg tcggcgatcg gcgccctcga gctgtcgaac gtcgacggcc
      661 tcgactgcaa ggccgcgatt gccacggccg ccgccgggtt acggttcagc taccgcgttc
      721 ctgttcccgc ctccgacgat ccggccgagg tcggctttac catgacccta ccgtacctgc
      781 tcgtaggctc ggccaggcca gtggggttgg ccgccttcgt cgaggcgccg gcctcggcca
      841 tcgtgacgct gctcggccgc tcgggagcgc cgctcgaggc cgcttggccc ctcgcggcga
      901 cgcgggtcgg cgcggcgctc ggagacgact acggcgccta cgccgagctg ccggccttcg
      961 acggcggctc ggccggggac tgggccgcgt acggattctg ggcggcgccc cgaggctcgc
     1021 cgctgtcggc gctcgccagc gtcgtcgaga ccgaggccga gcccggcggc ctcggctggt
     1081 acgacccgcc ggccccggcc gggcgacggg cctacggagc gctcggcgtc tccgtaggcg
     1141 ggcctgcctg ggcggccgcc ggggcggctg cggccagcct cggcttgccg ggccgagacg
     1201 cggcggccgg ccgcgccgag gcgcgcctgt cggtcgccgg gtaccgcctc gagctagtcg
     1261 cctcctgcgc cgaccggttc tgggtcgacg tcgacggcgg tcaggcaccc ttcctgaggg
     1321 ccgacctgag cctgcgccgc tcgaggtccg gcctggcctg ggccctgggc tggcgctacc
     1381 tccgcccgga tgcctatacg cccgaaggta cggccgtctc catgcgagcc tcctgcgact
     1441 tggccgcgtg gctgggcctg gcgagcgttc gggcgcgcct gagcctggcc ggctcggcgg
     1501 aggccggctc ggcggaggct ggctcggcgg aggctggctc gagcgtcgtc gagctcgacg
     1561 cccgctaccg tccggctccg gcgccgtggc tatcgctgcg cagttcctgg cgcgtggtag
     1621 gcggcgactc ggagcgcttc gacctgggag ccgccatatc ggcaggaagt cgctggcccc
     1681 ttgagctgga gtccggcctg cgcttcgtcc ccgaagggcg cttgtacaag gccggcctgt
     1741 ccttgggcgt ctccggagac cgcgcgtcgg t
//
LOCUS       DAOOLN010000387         1771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_85697_length_1771_cov_2.603147, whole genome shotgun sequence.
ACCESSION   DAOOLN010000387 DAOOLN010000000
VERSION     DAOOLN010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85697_length_1771_cov_2.603147"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1740)
                     /locus_tag="PLW80_05685"
     CDS             complement(<1..1740)
                     /locus_tag="PLW80_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66031.1"
                     /translation="MAAYESAGAKLAEAGAAYDEAYSQASRRYDDLEEANHAYAIEDA
                     IWRWASSAYLYASDEACSGSLYGDPAGELSYCEASLARASTAMAALRGLYDEGQEVRE
                     YASSEYRAAYDEYRESYRRLMLVRKAEAALADGLTLELAANEKAAAAYRAARSGLATG
                     RTVDDGFAVYLRIGEDGCIRLAYDASFAFSGAMGQSELSAFFSAQAQGDTGSRMSETA
                     FETNLDRLASWMTSVSFDRTRAERWGLARDYVVKRLAANDSGFASRVSYSESAIDDAT
                     IGGHRFTLAGPTLAEILATYREDSLAADQEAAYASLDGDEKAMFDTYLALQLTGAMKP
                     PKATNDGSSTYDAFSSWSDRAQYAALEAEAERQIAVCRTGIEASAATYAGFLAAAAVA
                     GAFLITIFLVPGLLAAAALALATMTAFGLTMSGVGETRAVYSSAMSDLFAQTESGALR
                     MTDGMPSCVALRSKYLSSCDRLASLEGVTLGGAACDESSLRAALAQSGSLTSAEVDEL
                     TSLYGAYASETGATNHGSLEALSALATWSRVSRDDSLGGLEAAYAEDEVERVEAGEIY
                     NAVYAAFIAGRASE"
BASE COUNT          319 a          591 c          560 g          301 t
ORIGIN      
        1 ttccgaggcg cgtccggcga tgaaggcggc atagacagcg ttatatatct ctcccgcctc
       61 gacgcgttcc acctcgtctt cggcatacgc cgcctccagt ccgccgagcg agtcgtcgcg
      121 tgacacccgg ctccatgtcg cgagcgctga cagagcttcg agcgaaccat ggttggtcgc
      181 cccggtttca gaggcatagg cgccatacaa cgaggtcaac tcgtcgacct cggcagacgt
      241 cagcgagccg gactgcgcca acgcggctct caggctcgac tcatcgcacg cggctccccc
      301 aagggtgacg ccctcgagcg aggccagccg atcgcacgag gataggtact tcgatcgtaa
      361 cgctacgcac gaaggcatgc cgtccgtcat gcgaagggca ccggactcgg tctgggcgaa
      421 taggtccgac atggccgatg agtagaccgc tcgggtctcg ccgacgccgg acatggttaa
      481 cccgaacgcc gtcatcgtgg ccaaggcaag agccgcggcg gctagtaacc cgggaacgag
      541 gaaaatggtg atcaagaagg ctccggcaac ggcggcggcg gcgagaaacc cggcgtacgt
      601 agccgcgctc gcttcgatgc cggtacggca tacggcgatc tgacgttcgg cctcggcctc
      661 gagcgcggca tactgggccc ggtcagacca actcgagaag gcgtcgtagg tcgacgaacc
      721 gtcgttggta gccttgggcg gcttcatcgc cccggtcagc tgcaacgcca gataggtatc
      781 gaacatggcc ttctcgtcgc catcgaggct ggcgtaggcg gcctcctggt cggccgcgag
      841 cgaatcctcc cgatacgttg cgagtatctc cgcaagcgta ggacccgcca gcgtgaagcg
      901 atgtcccccg atcgtggcgt cgtcgatggc cgattcggaa tacgaaactc gtgacgcgaa
      961 gcctgagtcg ttcgcggcta agcgcttaac gacgtaatcg cgggcgagcc cccagcgctc
     1021 ggccctggta cgatcgaacg agacgctcgt catccaggag gcaagcctat cgagattcgt
     1081 ctcgaacgcg gtctcggaca tcctcgaacc ggtatcgccc tgcgcctgag ccgagaagaa
     1141 cgccgacagc tcgctttggc ccatcgcgcc ggagaacgcg aaggacgcgt cgtaggccag
     1201 gcggatacag ccgtcctctc caatacgcag gtatacggcg aagccgtcgt cgaccgttcg
     1261 cccagtggcc aggcccgacc tcgcggctct gtaggccgcg gcggcttttt cattggcggc
     1321 caactcgagg gtcaggccgt cggccagggc cgcttcggct tttctgacga gcattaggcg
     1381 ccgatagctc tctcgatact cgtcgtacgc ggcacggtat tcgctcgacg catattcgcg
     1441 cacctcctgg ccctcgtcat agagcccgcg tagcgcggcc atggccgtag aggcacgagc
     1501 cagcgacgcc tcgcagtatg acagctcccc ggctggatcg ccgtacagcg agcccgagca
     1561 cgcttcgtct gaagcgtaga ggtaagccga actagcccaa cgccagatcg cgtcctcgat
     1621 ggcatacgcg tggttcgcct cctcgaggtc gtcgtatcgc cgcgaggctt ggctatacgc
     1681 ctcgtcatat gcggctccgg cctcggcgag cttggcgccg gcactttcgt acgcggccaa
     1741 ggcgactcgt tgccctccgc gcgcggcctc g
//
LOCUS       DAOOLN010000388         1767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_85987_length_1767_cov_2.327103, whole genome shotgun sequence.
ACCESSION   DAOOLN010000388 DAOOLN010000000
VERSION     DAOOLN010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85987_length_1767_cov_2.327103"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..295
                     /locus_tag="PLW80_05690"
     CDS             77..295
                     /locus_tag="PLW80_05690"
                     /inference="COORDINATES: protein motif:HMM:NF025742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HPB66032.1"
                     /translation="MKNSDDDIALKEEYDFSNGVRGRFYSPSKVSTSIRLDNDVILFF
                     KKKASEEKIGYQTLLNMALREYINHHLQ"
     gene            complement(493..>1767)
                     /locus_tag="PLW80_05695"
     CDS             complement(493..>1767)
                     /locus_tag="PLW80_05695"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPB66033.1"
                     /translation="GPDTEIFYWVGEGLPPASSNKGTDPANWMEIWNNVFMQFNRVDE
                     KTLQKLPKQNVDTGMGLERTVCVLNGKTSVYQTESFQPIIAAIEAVSGYAYGTDPARD
                     PSVRIIADHVRSATMILGDPKGVTPSNVGAGYVLRRLIRRAVRHARKLGIEGGFLGKP
                     AAVVVDLLSGAYPELADNRAKIIEELAREEAKFLETLQKGEHEYEKMLPNLLRNPQKV
                     MSGRLAFKLYDTYGFPIELTQELAAESGMTVDLAEFDAAFKKHQDLSRAGAEQTFKGG
                     LADQSEQATKYHTATHLLHKALRMVLGDHVAQKGSNITAERLRFDFSHSAPMTPDEVA
                     RVQAIVNEQIARDLPVTMEMMSLDDAKASGAMALFGEKYESVVKVYTIGDFSKEVCGG
                     PHVERIGALGRFVIQKEQSSSAGVRRIRAVLE"
BASE COUNT          281 a          538 c          616 g          332 t
ORIGIN      
        1 tactataccg acgacggcga cgaggtcgtg cgactcatct ccgccaggga ggctaccagg
       61 aatgagctca gacaatatga agaactcaga tgacgatatc gcgctgaagg aagaatacga
      121 tttctcgaat ggcgtccgcg gacgattcta tagcccgtcc aaggtatcga cgtccatacg
      181 tctcgacaac gacgtcatcc tcttcttcaa gaagaaggcc agcgaggaga agataggcta
      241 ccagaccctg ctgaacatgg cgctccgcga gtacatcaac caccatctac agtagcgcga
      301 tcgccgactc atcgcggcga tcgccggccg tcgttcgacc tgactcgctc tcgctcgcag
      361 gtcaacgacg gcggccctcg cgtgagcgag ggtcccgcct cttgggcagg accctccgag
      421 atgctatacg aggcctgaat acattcccga gcctcagggg cggcgcgtcg tgtcccgctc
      481 cgcggcgcgt cgctactcga gcacggctcg tatccggcgg acgccggccg acgagctctg
      541 ctccttctgg atgacgaacc tgcccagggc gccgatgcgc tcgacgtggg ggccgccgca
      601 gacttccttg gagaagtcgc cgatggtgta gaccttgacg accgactcgt acttctcccc
      661 gaacagggcc atggcgccgg aggccttggc gtcgtcgagg ctcatcatct ccatggtcac
      721 gggcaggtcg cgggcgatct gctcgttgac gatggcctgg acgcgggcca cctcgtcagg
      781 cgtcatcggg gccgaatggg agaagtcgaa gcgcaggcgc tcggcggtga tgttggagcc
      841 cttctgggcc acgtggtcgc ctaagaccat gcgcagggcc ttgtggagga ggtgcgtggc
      901 ggtgtggtac ttggtcgcct gctcggactg gtcggcgagg ccgcccttga aggtctgctc
      961 ggcgccggcg cgggacaggt cctggtgctt cttgaaggcg gcgtcgaact ccgccaggtc
     1021 tacggtcatg ccgctctcgg cggcgagttc ctgggtcagc tcgatcggga agccgtaggt
     1081 gtcgtacagc ttgaaggcga gccggccgga catgaccttc tgcgggttcc gcagcaggtt
     1141 cgggagcatc ttctcgtatt cgtgctcgcc cttctgcagg gtctcgagga acttggcctc
     1201 ctcgcgggcc agctcctcga tgatcttggc gcggttgtcg gccagctcgg ggtaggcgcc
     1261 ggagagcagg tcgacgacga cggcggctgg cttgccgagg aagccgccct cgatgccgag
     1321 cttcctcgcg tggcggacgg cgcggcggat gagccgcctg agtacgtagc cggcgccgac
     1381 gttggacggg gtgacgccct tggggtcgcc caggatcatc gtcgcggagc ggacgtggtc
     1441 ggcgatgatg cgcacggacg ggtccctggc ggggtcggtc ccgtaggcgt agccggagac
     1501 ggcctcgatg gcggcgatga tgggctggaa gctctcggtc tggtagacgc tggtcttgcc
     1561 gttgagcacg cagacggtgc gctcgaggcc catgccggtg tcgacgttct gcttggggag
     1621 cttctggagg gtcttctcgt cgacgcggtt gaactgcatg aagacgttgt tccagatctc
     1681 catccagttg gcggggtcgg tgcccttgtt ggagctggcc ggcggcaggc cctcgcccac
     1741 ccagtagaag atctcggtgt cgggccc
//
LOCUS       DAOOLN010000389         1762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_86367_length_1762_cov_2.223199, whole genome shotgun sequence.
ACCESSION   DAOOLN010000389 DAOOLN010000000
VERSION     DAOOLN010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86367_length_1762_cov_2.223199"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="PLW80_05700"
     CDS             <1..649
                     /locus_tag="PLW80_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB66034.1"
                     /translation="KPRVLVLVGPTGVGKTTTIAKLAALHSLGIGGLPAGAVRMITID
                     SYRIGAVEQIEKYAGIMRVPVSVVETAQDLRETIAMYRDNDLILVDTIGKSPRDAIRL
                     AEMQQVLDACGPAAEVYLAVAATTKTQDMLDIFRQFEPFRYRGVVVTKMDETSRVGNV
                     LSVLAETDKAVAWITDGQKVPQDIARANPLRFLMNLEGFRPNRPKLEERYGSTTT"
     gene            675..1541
                     /locus_tag="PLW80_05705"
     CDS             675..1541
                     /locus_tag="PLW80_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002680723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HPB66035.1"
                     /translation="MEDQAQKLRELMSAKPQPQQAAKKTRVITVASGKGGVGKTNLSV
                     NLAIAYARTGKRVMVMDADLGLANVNIMLNMIPKYNLYHVIRKQKSMRDIILDTEYGI
                     QILAGASGFSKIANLNEEERQNFISELYALTNVDIIIIDTSAGVSNNVLSFVAAADDA
                     IIITTPEPTAITDAYGIIKIIATEVENLNIGLKLVVNRVKTVTEGRKVAERIINISGQ
                     FLNLKVEYLGCIYEDPIVQQSVLRQRPFIVQDPKSKAAISVQHLVNRIEKTEFVDDRG
                     ISRLIKKLFSKE"
BASE COUNT          384 a          537 c          526 g          315 t
ORIGIN      
        1 caagccgcgg gtgctcgtgc tcgtggggcc gaccggcgtc ggcaagacga cgaccatagc
       61 caagctggcc gccctgcaca gcctcggcat aggcggcttg cccgccggcg ccgtgcggat
      121 gataacgata gacagctacc gcatcggggc cgtcgagcag atcgagaagt acgccgggat
      181 catgcgcgtg ccggtctccg tggtcgagac ggcccaggac ctgcgcgaga ccatagcgat
      241 gtaccgtgac aatgacctta ttctggtcga tacgataggc aagagccccc gggacgcgat
      301 acggctggcc gagatgcagc aggtgctcga tgcctgcggg ccggccgccg aggtctacct
      361 cgcggtggcc gctacgacca agactcagga catgctcgat atcttcaggc agttcgagcc
      421 gttccggtat cggggtgtgg tcgttacgaa gatggacgag acgagccgcg tcggaaacgt
      481 gctttcggtg ctcgctgaga ccgacaaggc cgtggcctgg attaccgacg gccagaaggt
      541 gccccaggac atcgcgcggg caaatcctct cagattcctc atgaatctcg aaggttttag
      601 gccgaatcga ccgaaactag aagagagata cggatcaacg actacgtaac ccgccgtatc
      661 gatggagtgc tcgtatggaa gaccaggcgc agaagctgcg ggagctgatg agcgctaagc
      721 cgcagcccca gcaggcggcc aagaagaccc gtgtcatcac cgtcgccagc ggcaagggcg
      781 gcgtcggtaa gacgaacctg tccgtcaacc tagccatagc ctacgcccgg accggcaagc
      841 gcgtgatggt aatggacgcg gacctcggcc tggccaacgt caacatcatg ctcaacatga
      901 ttcccaagta caacctgtac catgtgatac gtaagcagaa atctatgcgc gacatcatcc
      961 tggatacgga gtacgggatc cagatcctgg ctggggcgtc gggcttctcc aagatcgcca
     1021 acctgaacga ggaagaacgg cagaatttta tatccgagct gtacgctctg actaatgtcg
     1081 acatcatcat catcgatacg agcgccggcg tctcgaacaa cgtgctgtcc ttcgtcgccg
     1141 cggccgacga cgctatcatt atcaccaccc cggagccgac cgccattacc gacgcctacg
     1201 gcatcatcaa gatcatagcc accgaggtcg agaacctgaa cataggcctc aagctggtcg
     1261 tcaaccgcgt gaagaccgtg accgaaggcc gcaaggtggc cgaacgtatc atcaacatct
     1321 cggggcagtt cctcaacctg aaggtcgaat acctcggctg catctatgaa gaccccatcg
     1381 tccagcagtc ggtgctcagg cagcggccct tcatcgtcca ggatcccaag tccaaggccg
     1441 caatctcggt gcagcacctg gtgaaccgta tcgagaagac cgaattcgtc gacgaccgcg
     1501 gcattagccg cctcatcaag aagctgttct cgaaagaata gcgccgcgga gcgtaccgag
     1561 agccaaagct tgacgagaca aggtattatg ccttaatgtt gtcggctatg ggagggtact
     1621 gaaacggtgg actcgagtct tcgcgcgtcc gccttcgcgg ggcttggcgc gttcgcgtta
     1681 tcgatcatcg tcgccgcttt ctcgcgcgtc cctttcggcg cgttggtcct ccgcgccctg
     1741 ctgtcgggcc tagccttcgc gg
//
LOCUS       DAOOLN010000390         1758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_86646_length_1758_cov_3.526130, whole genome shotgun sequence.
ACCESSION   DAOOLN010000390 DAOOLN010000000
VERSION     DAOOLN010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86646_length_1758_cov_3.526130"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="PLW80_05710"
     CDS             complement(<1..881)
                     /locus_tag="PLW80_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPB66036.1"
                     /translation="MNEMLLEKAAPAKRSKKTRSVLQQKKLKRPKTTKESDPLALYLK
                     QISRYPLLSAEEEVELGGAIQKTRNAVAALDSIADESDPDYGRNRAARAEKQAELVAY
                     KHRMISSNLRLVVSIAKNYQHRGLSLLDIIDEGNIGLIEAVERFDYTRGCRFSTYGTW
                     WIRQAIIKSLADKGRVIRIPIHMLNTIKKCYFVAKQLTQDLGRDPYAAELADKLGMDP
                     KKVKEIMKLSQETASLDTTVDEDAVTHLADLIKDENAVEPFEEVFCMTLQDTISDVLK
                     NLNQREITIITLRFGLNG"
     gene            complement(1093..>1758)
                     /locus_tag="PLW80_05715"
     CDS             complement(1093..>1758)
                     /locus_tag="PLW80_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPB66037.1"
                     /translation="AGRLFVGMQLAIPGGSDTEPRRLDYVVKKGDTLYGIAKAHGVAV
                     DDIVKASGMSTTTIKIGQTLRIPTPSADAPAASVSTTPASPAKPVSVAVPPASVAASA
                     KPAIAAGLSWPAAGSVSYLQGKLKGVSIAAEPASSITAVRSGTVISAGPFRGFGLVAY
                     VQAVDSKIYVYGGAAALSVKLGDPVRKGSLIGKVSSDGDASAYFFVFDGADTIDPASA
                     PRD"
BASE COUNT          339 a          515 c          522 g          382 t
ORIGIN      
        1 ccgttcagtc cgaaacgcaa cgtgatgatc gtgatctcgc gctggttgag attcttgagc
       61 acgtcgctga tggtatcctg caacgtcatg cagaagacct cctcgaaggg ctcgacggcg
      121 ttctcgtcct tgatcaggtc cgccaggtgg gtgaccgcat cctcgtcgac cgtcgtatcg
      181 agcgaagccg tctcctggga cagcttcatg atctccttga ccttcttggg atccatgccg
      241 agcttgtcgg ccagctcggc ggcgtacgga tcgcgcccga ggtcctgggt aagctgcttc
      301 gcgacgaaat agcacttctt gatggtgttg agcatgtgga tcgggatgcg tatgacgcgc
      361 cccttgtcgg ccaggctctt gatgatggcc tggcgaatcc accaggtacc gtaggtggaa
      421 aacctgcaac cgcgcgtata gtcgaagcgc tcgacggcct cgatcaggcc gatgttgcct
      481 tcgtcgataa tatcgagcag ggacaggcct cggtgctggt aattcttggc gatcgagacg
      541 acgaggcgca ggttgctcga tatcatgcgg tgcttgtagg cgacgagctc ggcctgcttc
      601 tccgcccttg cggctcggtt gcgaccgtag tccggatcgg actcgtcggc tatcgagtcg
      661 agcgcagcca cggcgttcct cgtcttctgg atggccccgc cgagctccac ctcctcctcg
      721 gcgcttaaca gcggatacct agaaatctgt ttgaggtaca aggcgagcgg atcgctttcc
      781 ttggtagttt tcggtcgttt cagctttttt tgctgtagca ctgatcgcgt ttttttagat
      841 cgcttcgcgg gggctgcctt ctcaagcaac atttcgttca tacggtctcc tgaatcaggc
      901 cgctaagcct aggccgtggc gaataaccct cgtaggcatc gaatcgcaga tggaatttta
      961 tacatccgga ggaataaatc aagggaaaat gagatttttt atcaaaaata ttaaatttca
     1021 ctatatcaaa gcctagattg taaaagaata tttaccgatt gtaccgtcga gactcgatgt
     1081 ctaccagata ggctagtccc gaggtgcgga tgccggatcg atcgtatcgg cgccatcgaa
     1141 gacgaagaag taggccgacg catcgccgtc cgatgagacc ttgccgatca gcgaaccctt
     1201 acgcactggg tctccgagct tgaccgatag cgccgccgcg ccgccgtaga cataaatctt
     1261 gctatccacc gcctgtacat acgcgaccaa accgaagcca cggaacggtc ccgccgatat
     1321 gaccgtaccc gacctgaccg cggtaatgga cgaggccggt tcggcggcta tcgacacgcc
     1381 cttgagctta ccttgcaggt acgagaccga tcccgcggcc ggccagctca agccggccgc
     1441 tatggcgggc ttcgcgctcg ccgctacgga ggccggcggg acggcgaccg acacaggctt
     1501 tgccggtgac gccggcgtcg tcgagacgga ggccgccgga gcgtccgccg aaggcgtcgg
     1561 gatacgcaga gtctggccga tcttgatggt cgtcgtggac atgccgctcg ccttgacgat
     1621 gtcgtcgacg gcgacgccgt gagccttggc gatgccgtag agcgtgtcgc ccttcttgac
     1681 gacgtagtcg agccgacgag gttccgtgtc cgaaccgccc ggaatggcca gctgcatgcc
     1741 gacgaacaac ctgcctgc
//
LOCUS       DAOOLN010000391         1757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_86728_length_1757_cov_2.781434, whole genome shotgun sequence.
ACCESSION   DAOOLN010000391 DAOOLN010000000
VERSION     DAOOLN010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86728_length_1757_cov_2.781434"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PLW80_05720"
     CDS             <1..211
                     /locus_tag="PLW80_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66038.1"
                     /translation="TLSKKVLDAVEAGRFAIYAVSTIDEGIEVLADRAPGAPDALGRF
                     PKGSFNGLVAEELRRMAETIKEYRE"
     gene            259..1617
                     /locus_tag="PLW80_05725"
     CDS             259..1617
                     /locus_tag="PLW80_05725"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HPB66039.1"
                     /translation="MFAACAALLAGSCGGGGSREPAAGQAPAEAAQPSESRAAEASAP
                     DAGAPSDAAQAGRPAPDAAAGAAATGTATEAPPGEPAATEGAGAAQATGSEPSRSAPR
                     RLGELVFTEGAVTLHRAGSSPRRADIGDVVAAYDLIATGPGARAEVDIGSGAAGGATV
                     KLTENTAFYFDTKELDDAARRTVLQLLSGAVAIKVDRLATGSFSVTTDSAVLGVRGTV
                     FIVDTVPDGSMLVSCDTGAVSVRSDGAVTTAKPGGVVELTDAGGLRLLAVQPDALADY
                     RGAWRDEAYKGFAKRALSYTSAYAKTLDGGAPTLAAAVSRLRAQQPALDAWRAARAAG
                     RVPRFTDWTAEKKAVGAALFDCLKAAFAVERPYYRLLELEALHDAGVGVGALSDGRKS
                     SSFFGSFGKDYAGLAQDLAAVREALALFSWAAAGSPLGDFFGAKAEALGTGAALFEDE
                     DW"
BASE COUNT          239 a          660 c          620 g          238 t
ORIGIN      
        1 gacgctttcg aagaaggtgc tcgacgccgt cgaggccggg cgcttcgcca tctacgccgt
       61 ctccaccatc gacgagggca tcgaggtgct cgccgaccgg gcccccggag ccccggacgc
      121 gctcggccgc tttcccaagg gcagcttcaa cgggctcgtg gccgaggagc tcagacgcat
      181 ggcggagact ataaaggaat atcgcgaatg agatcgacta tggggacttc gcgtcacgcg
      241 agtggtattg gcatcgcgat gttcgccgcc tgcgcggcgc ttctagccgg ctcgtgcgga
      301 gggggcggct cgcgcgagcc ggccgccggc caggctccag ccgaggccgc ccagccatcg
      361 gagtcccgag ccgccgaggc atcagcgccc gacgccggcg ccccgagcga tgcggcccaa
      421 gccggccgtc cggccccgga cgccgccgca ggcgccgcag ccaccgggac cgctaccgag
      481 gcgcctcccg gcgagcccgc cgcgaccgag ggcgccggcg cggcccaggc gacgggttct
      541 gaaccgtccc ggtcggcgcc gcggcgcctg ggcgagctcg tcttcaccga gggcgccgtg
      601 acgctccacc gcgccggctc gtcgccgcgg cgcgccgaca tcggcgacgt ggtggcggcc
      661 tacgacctga tcgcgaccgg ccccggcgcc cgcgccgagg tggatatagg ctccggcgcg
      721 gccggcggcg ccaccgtcaa gctgacggag aatacggcct tctacttcga tacgaaggag
      781 ctcgacgacg cggccaggcg caccgtgcta cagctgctat ccggcgccgt ggcgatcaag
      841 gtggatcggc tggccaccgg ctccttcagc gttaccaccg actcggcagt gctcggcgtg
      901 cgcggcaccg tcttcatcgt cgacaccgta cccgacggct ccatgctcgt ctcctgcgac
      961 acgggcgccg tatcggtgcg ctccgacggc gccgtgacca ccgccaaacc aggcggcgtg
     1021 gtcgagctga cggacgcggg cggcctcagg ttgctcgccg tccagccgga tgccttggcg
     1081 gattatcgcg gcgcttggcg cgacgaggct tacaagggct tcgccaagcg cgcgctatcg
     1141 tacaccagcg cctacgccaa gaccctcgac gggggcgccc cgaccctcgc cgcggccgtc
     1201 tccaggctac gcgcgcagca gccggccctg gacgcgtggc gcgccgcccg cgccgccggc
     1261 cgcgtaccgc gctttaccga ctggaccgcc gagaagaagg ccgtgggtgc ggccctgttc
     1321 gactgcctca aggccgcctt cgcggtcgag cgcccctact accgcctgct cgagctcgaa
     1381 gccctccacg acgcgggcgt cggcgtcggc gccctctccg acggtcgcaa gagctcgtcg
     1441 ttcttcggct ctttcggcaa ggactacgcc ggcctcgccc aggacctggc ggcggtacgc
     1501 gaagccctgg cgctgttctc atgggccgcg gcgggcagcc cgctcggcga cttcttcggc
     1561 gccaaggccg aggccctggg taccggcgcc gccttgttcg aggacgaaga ctggtaagcc
     1621 cggcccagga tgcggccctc ggggctgccc gcccgataac ggctcgggct ggccggcgtc
     1681 ggatggaata gcgtcccggc gtagccggga cgaggcgccg gacggcgccc ttatcgatgg
     1741 tagatcgtcg cgcggcc
//
LOCUS       DAOOLN010000392         1757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_86749_length_1757_cov_2.237368, whole genome shotgun sequence.
ACCESSION   DAOOLN010000392 DAOOLN010000000
VERSION     DAOOLN010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86749_length_1757_cov_2.237368"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1633)
                     /locus_tag="PLW80_05730"
     CDS             complement(<1..1633)
                     /locus_tag="PLW80_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66040.1"
                     /translation="MRRTNLNALGFDTLRLEGSLFVADYLEKAATGLASGQNAADYRV
                     PRGLRLNDEYGRAFQIAQAIWSQNEVAPSRLESSVVELFRDALGYSPIDKVAPVAVGE
                     RRFPIGHLALEKVPLVIGSPGSDLDASEARFSAEGSGSRKKSPFQMAQEYLNARAGAS
                     GDSSGLPGQAPLWAIVTNGERIRLLRASPAMARPSYLEFDLGTIMREKRFPEFTMLWR
                     LLHASRVGVGDGGTVWEAWRQEGENQGTRVREGLKAGVTQALLSLGSGFLRHKGPGNV
                     ALRAALDDGSLGREAYFQELLRLIYRFLFVFTIEERGLIHAHPGDYTRAASHELYERG
                     YSLRRLRDKALRRSGYDRHGDLWEGVGVLFRALGTGEDALDLPALGGLFAPDQCPTID
                     ACGLSNYDLLSAMESLRWAVAGNARTVVDYKNIDSEELGSVYESLLELVPVVDLAARS
                     FGFVGVGGEEGSTAGNARKTSGSYYTPDSLVQELIKSALEPVIEARLRGESDPAKKEA
                     AILGITVIDPSCGSGHFLLAAGRKLAETLALVRSED"
BASE COUNT          307 a          575 c          565 g          310 t
ORIGIN      
        1 cgtcctcgct gcgaaccagg gccagggttt cggcgagctt ccgtcccgcg gccaggagga
       61 agtgtcccga tccgcaggaa gggtctatca cggtgatccc gaggatggcc gcttcttttt
      121 ttgcagggtc gctttctccc ctgagcctcg cctcgatcac cggctccaaa gcgctcttga
      181 tgagttcctg tacgaggctg tccggcgtgt agtagcttcc tgaggtcttg cgcgcattgc
      241 ccgccgtcga tccttcttcg ccgcccacgc cgacaaagcc gaagctccgc gcggccaggt
      301 cgacgaccgg cacgagctcg agaaggctct cgtacaccga gcccagttcc tcggagtcta
      361 tgttcttgta gtctacgacg gtgcgcgcgt tgcccgcgac agcccagcgg aggctctcca
      421 tggcggagag gaggtcgtag ttggaaagcc cgcaggcgtc gatagtaggg cattggtcgg
      481 gagcaaagag tccacccagg gcgggcaggt cgagggcgtc ctctcccgtt ccaagggcgc
      541 ggaagagcac cccgacgcct tcccagaggt cgccgtggcg gtcgtagccc gaacgcctga
      601 gcgccttgtc ccgcaggcgc ctgagcgagt agccgcgctc gtaaagctca tgggatgctg
      661 cgcgcgtata atcgcccgga tgagcgtgga taaggccgcg ctcttcgatg gtaaagacga
      721 agaggaagcg gtaaatgagc cgcaagagtt cctggaagta cgcttcccgg cccagggagc
      781 catcatcgag ggccgcgcgc agcgccacat tgcccggccc tttatgacgg agaaagcccg
      841 aaccaagcga cagaagtgcc tgagtgacac cagccttgag cccctcgcgc acgcgggtgc
      901 cctggttctc gccttcctgc cgccacgctt cccataccgt gcctccgtcg cccactccca
      961 ccctgctcgc gtgcaggagc cgccagagca tggtgaactc gggaaagcgc ttctcgcgca
     1021 tgatggtccc gaggtcgaac tcgagatagg aaggtcgcgc catggccggg gatgcgcgga
     1081 gcaggcgtat acgctcgccg ttcgtgacga tagcccaaag cggagcctgg ccgggcaagc
     1141 ccgaggaatc gcccgaagcg cccgctcgcg cgttgagata ctcctgcgcc atctggaagg
     1201 ggcttttctt tcgggagccc gagccttccg cggaaaaccg ggcctcgctc gcatcgagat
     1261 cggagccggg gctaccgatg accaggggaa ccttctcgag cgcgaggtgg ccgatgggga
     1321 aacgacgctc gcccacggct accggcgcca ccttatctat gggcgaatac cccaaggcgt
     1381 cgcggaagag ctcgacgacc gagctctcca gcctcgacgg cgcgacctcg ttctggctcc
     1441 agatggcctg ggctatctga aaggcccgtc cgtactcatc attcaggcgc aagccgcggg
     1501 gcacgcgata gtcggccgcg ttctgcccgc tcgccaggcc cgtcgcggct ttttccaggt
     1561 agtcggctac gaagagcgag ccctcgaggc gaagcgtatc gaatcctagc gcgttcaggt
     1621 tggtgcgtct cattttttca tccctttaga ttgcatttgg caggaggacg tacacgccga
     1681 tgaggtccac cggaaggcag gcggtgacgc catactgccc cgtgtcgccc gccgcgtcgc
     1741 gtacgcgccg gtgatcc
//
LOCUS       DAOOLN010000393         1751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_87198_length_1751_cov_2.971698, whole genome shotgun sequence.
ACCESSION   DAOOLN010000393 DAOOLN010000000
VERSION     DAOOLN010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87198_length_1751_cov_2.971698"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PLW80_05735"
     CDS             <1..642
                     /locus_tag="PLW80_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPB66041.1"
                     /translation="RSISSSIDTMADRFKDLLRSADDGKRTQETARERIQLIVDRSKS
                     LLEANTVIATIASQTNLLAMNAAIEAAHAGDMGKGFAVVADEIRRLAETSAIQSKAIT
                     SDLGAVQAVIESVVVSSNESTESFSTVASRIVGLDSLVHQVDLAMAEQKAGTSKVLEA
                     LQSMNEITHQVKSGSAEMSAGNGIVLSEISRLQDSTRQIIASVEDMSGGVRRL"
     gene            complement(802..>1751)
                     /locus_tag="PLW80_05740"
     CDS             complement(802..>1751)
                     /locus_tag="PLW80_05740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HPB66042.1"
                     /translation="GWPEIADPSGIDPCCDVYQDRRAGITQCKETDGRENGTDGEESE
                     RRETVDYRAEPATLAHDGSWPGFLCACAEALNASEPVPRVVDADAPVGLFESRIAVAR
                     DDGRAAALWDRLTIKAGAPAMRIVLEAFLSERPDARADTASVLRRLRREGAAALRDLA
                     DPIVLSVDKAATRARREAERTTGIARFSELADGSWYAPLEPGCDVLPLVADHFAARFR
                     PMRFALHDLRRGRAVLHEPGRPWLLTDGFSLKRVEPDEDAGPGAIDSAMLASDELRIR
                     DLWRRYFAATAIEGRLNPGLQAAHIPKKYWKLLTEMRRG"
BASE COUNT          304 a          573 c          582 g          292 t
ORIGIN      
        1 aggtcgatct cctcgtcgat cgataccatg gcagaccgat ttaaggatct gctacggtcc
       61 gccgacgacg gcaagaggac ccaggaaacc gcgcgcgagc gtatccagct gatcgtcgat
      121 cgctccaagt cgctgctcga ggccaatacc gttatcgcga ccatcgcgtc gcagaccaat
      181 ctgctggcga tgaacgccgc gatcgaggcc gcccatgccg gagacatggg caagggtttc
      241 gccgtcgtgg ccgatgagat ccgcaggctc gcggagacct cggcgattca gtcgaaggcc
      301 atcacgtcgg atctcggcgc ggtgcaggcc gtgatcgagt cggtggtcgt ctcgtcgaac
      361 gagtcgaccg agtcgttctc gacggtggcg tcgcgtatcg tcggcctcga ttccctggtt
      421 caccaggttg acctagccat ggccgagcag aaggccggca cgtccaaggt gctcgaggcc
      481 ctgcagtcca tgaacgagat aacgcatcag gtcaagtcgg gctcggccga gatgagcgcc
      541 gggaacggca tcgtcttgtc ggagatatcg aggctgcaag actcgacgag gcagatcata
      601 gccagcgtcg aggatatgtc cggtggggtc aggcgtctct aggagcgcgc cgcgatgata
      661 tcgacgatag cccatgatac cgaggcgact atcggcggca tggacgaagc cctgcggggt
      721 ttcaggacgg agggctccga gcttggagcg acccggcatt cctgagcgtc aagatcggcc
      781 ccaagtcagt gtccgtagcc gctatccccg gcgcatctcg gtaaggagct tccagtactt
      841 cttcgggata tgggcggcct gaaggccagg gttgagccgc ccttcgatgg ccgtggccgc
      901 gaagtatcgc cgccagaggt cgcggatgcg caattcatcc gaagccagca tggccgaatc
      961 gatcgcgcca ggcccggcgt cttcgtcggg ctctacccgt ttcagggaga agccgtccgt
     1021 cagcaaccag gggcggcccg gctcgtgtag cacggctcgg cccctgcgca ggtcgtgcag
     1081 agcgaatcgc atcggcctga agcgggccgc gaaatgatcc gcgacgaggg gtaggacgtc
     1141 gcatccaggc tcgagcggcg cgtaccagga gccgtcggcc agctccgaga agcgcgcgat
     1201 gcccgtcgtt cgttcggcct ccctgcgcgc cctggtagcg gccttgtcga ccgacaggac
     1261 gatggggtcg gccaggtcgc gcagggcagc ggcgccctcg cggcgaaggc gccgcaggac
     1321 ggaggccgtg tcggcccggg cgtcgggccg ctccgacaga aaggcctcga gcacgatacg
     1381 catcgccggg gcgcccgcct tgatcgtcag cctgtcccac agggccgcgg ctcgcccatc
     1441 gtccctcgcc acggcgatac gcgattcgaa cagccccacc ggcgcgtccg catcgacgac
     1501 gcgcggaacc ggctcggacg cgttgagcgc ctcggcgcac gcgcagagga agcccggcca
     1561 gctaccgtcg tgggccagcg tcgccggctc ggcccgatag tccaccgtct ctcggcgctc
     1621 ggactcctca ccgtcagttc cattctctcg accatcggtc tccttgcact gggttatacc
     1681 agctcgcctg tcctggtata cgtcgcagca gggatctatg ccactgggat ctgctatttc
     1741 tggccaacca t
//
LOCUS       DAOOLN010000394         1747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_87481_length_1747_cov_3.372340, whole genome shotgun sequence.
ACCESSION   DAOOLN010000394 DAOOLN010000000
VERSION     DAOOLN010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87481_length_1747_cov_3.372340"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..956
                     /gene="proC"
                     /locus_tag="PLW80_05745"
     CDS             156..956
                     /gene="proC"
                     /locus_tag="PLW80_05745"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HPB66043.1"
                     /translation="MGRTIGFIGAGKIVDIMLGALCPAFGDDELWVWNRTASRSEALA
                     ARYPVSVSPSVKDLAERSDWLFVAVKPYAVAKVLSEACLSVRDDALVISMAAGVTIDA
                     IKTHFAAPPAIVRIMPNLPLKAGCGMTAICPDPSVKQADIDALLALFSSVGRARLVEE
                     RLFDTVTALSGSGPAFAFLFIESMADGAVLNGMDRAQAYEFAAQTVMGAAKIILESDR
                     HPGELKDIVASPAGTTMAGIHSLERKAFRAIVMDAVDAAAARSRELSS"
     gene            complement(1026..>1747)
                     /locus_tag="PLW80_05750"
     CDS             complement(1026..>1747)
                     /locus_tag="PLW80_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66044.1"
                     /translation="ETEYWPILAFSALIAGIDSGALPVAAFAVSGPAGDVIARAFPRL
                     LPAYSEPEAGSTASRAAPTATVNSDGSVVMSWTGDDELVSSNPTVTLSGSLAYTVSAD
                     KNEELTSIAMDASLKADLEAEIGAYAAAGLKGARMNLKAKGDASLTGSVSGFTAKGYA
                     ALSLNAGMSLDEAAGHKGGKYIVSVKFAESYDMAITLTDVAGSAAGEVEMSLVVKVYG
                     NDNKLIDEYEFAAEDFADVLF"
BASE COUNT          274 a          605 c          585 g          283 t
ORIGIN      
        1 tgccgaatcc agcttcctcg acgagggctc cgccacggtg ctggccgaag cctgcctcga
       61 cgaggagctg gcggctcacc gcggcttcag gttcttcctg gccgcctgcg cggtcgaggc
      121 gcgaatcgca tcgggacagc cgaaaggatg acactatggg taggacgata ggattcatag
      181 gagccggcaa gatcgtcgac atcatgctcg gcgcgctctg cccggccttc ggcgacgacg
      241 agctatgggt ctggaaccgg accgcctcgc ggtcggaggc gctagccgcc cgctacccgg
      301 tcagcgtatc gccaagcgtc aaggacctgg ccgagcgttc cgactggctc ttcgtcgccg
      361 tcaagcccta cgcggtggcc aaggtcttgt ccgaggcctg cctgagcgtg cgagacgacg
      421 ccctggtcat ctcgatggcc gccggcgtca ccatcgacgc gatcaagacg cacttcgcgg
      481 cgcccccggc catcgtgcgc atcatgccga acctgccgct caaggccggc tgcggcatga
      541 ccgccatctg cccagaccct tccgtcaagc aggcggacat cgacgccctg ctggcgctgt
      601 tctcgtcggt cggccgggcc cggctcgtcg aggagcggct gttcgacacg gtcaccgccc
      661 tgtccggctc ggggccggcc ttcgccttcc tgttcatcga gtcgatggcc gacggggccg
      721 tgctcaacgg catggaccgg gcccaggcct acgaattcgc ggcccagacc gtgatggggg
      781 ccgccaagat catcctcgag tccgaccgcc accccggcga gctcaaggac atcgtcgcct
      841 cgccggccgg caccacgatg gccggcatcc acagcctcga gcgcaaggcc ttccgcgcca
      901 tcgtgatgga cgccgtcgac gcggccgcgg cgcgtagccg cgagctgagc tcctaaaaaa
      961 aagcccgggg aacgggcccc gggcatcgct cggccccggc gactgagcgc cggagccttc
     1021 atggatcaga acagtacgtc ggcgaagtcc tcggcggcga actcgtactc gtcgatcagc
     1081 ttgttgtcgt tgccgtagac cttgaccacg aggctcatct cgacctcgcc agcggccgag
     1141 ccggcgacgt cggtcagcgt gatggccatg tcatagctct cggcgaattt gaccgagacg
     1201 atatacttgc cgcccttgtg gccggccgcc tcgtcgaggg acatgcccgc gttgagcgac
     1261 agggcggcgt agcccttggc cgtgaagccg cttaccgaac cggtgagcga cgcgtcgccc
     1321 ttggccttca ggttcatcct ggcgcccttg agcccggccg ccgcgtacgc gcctatctcg
     1381 gcctcgaggt cggccttcag ggacgcgtcc atggcgatgg acgtcagctc ttcgttctta
     1441 tcggccgata cggtataggc gaggctgccg ctcagcgtga ccgtcgggtt cgacgagacc
     1501 agctcgtcgt cgcccgtcca gctcatcacg acgctaccgt cggagttgac ggtggcggtc
     1561 ggagcggcgc gagacgccgt ggaaccggcc tcgggctccg agtacgcggg tagcaacctc
     1621 gggaacgcgc gggctatcac gtcgccggcg gggccggata cggcgaacgc ggcgacgggc
     1681 agggcgccgg aatcgatgcc ggcgatcagg gccgaaaagg cgagtatggg ccagtattcc
     1741 gtctcgc
//
LOCUS       DAOOLN010000395         1743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_87791_length_1743_cov_3.226896, whole genome shotgun sequence.
ACCESSION   DAOOLN010000395 DAOOLN010000000
VERSION     DAOOLN010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87791_length_1743_cov_3.226896"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="PLW80_05755"
     CDS             <1..936
                     /locus_tag="PLW80_05755"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPB66045.1"
                     /translation="EVGRLGTGRREIGHGHLARRALERVLPTKEQFPYTIRVVSDILE
                     SNGSSSMASVCGGCLSLLAAGVPIKAPVAGIAMGLITEGDRYAVLSDILGDEDHLGDM
                     DFKVAGTEKGITGFQMDIKIAKVSTEILTKALDQARRGRLHILGVMNQTIAAPAKEIS
                     EYAPKILSVKIDVDKIGAIIGPGGKNIKALCEQYGVRINVDDDGTVTIFGANQKASLE
                     AKAAVLGIAQDPEVGIVYQGTVKRVMDFGAFVEILPGKEGLVHISRLSRTRINAASDV
                     VKERQVIPVRLLEVDKLGRLNLSYIDAMDDAGQKK"
     gene            1020..>1743
                     /locus_tag="PLW80_05760"
     CDS             1020..>1743
                     /locus_tag="PLW80_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPB66046.1"
                     /translation="MLRDDTGIIFNRELVPGFRLLADEMPGATGVAIGLWFPVGSALE
                     RDGERGLSHFVEHMVFKGAGDRDAAELSRSIDRVGGYLNAFTEREAVCLHCLVPARHA
                     ELAVDILMDMAYRPRLSAVEFEREKDVIVNEILAAEDDLEEAAQDEFFALAYDGHPAA
                     RRIAGEVGDIREAVFSSLAEFHDERFSRGPVTLTVSGAVQAEALAERVVAALPAQRRS
                     ASGLAEAWDPGEPRFVRCRRMVK"
BASE COUNT          315 a          550 c          581 g          297 t
ORIGIN      
        1 gaggtaggac gcctcggcac cggccgccgc gagataggcc acggccacct ggccaggcgc
       61 gcgctcgagc gcgtgttgcc gaccaaggag cagttcccct atacgatccg cgtcgtctcc
      121 gacatcctcg agtcgaacgg ctcgtcctcg atggcctcag tctgcggcgg ttgtctatcc
      181 ctgctcgcgg ccggcgtgcc gatcaaggcg cccgtggcgg gcatcgcgat gggactgatc
      241 accgaggggg atcgctacgc ggtactctcg gatatcctcg gagacgagga ccacctgggc
      301 gatatggact tcaaggttgc cggtaccgag aagggcatca ccggcttcca gatggacatc
      361 aagatcgcca aggtgtcgac cgagatcctg accaaggccc tcgaccaggc ccgccgcggc
      421 agactgcata tcctgggcgt catgaaccag accatcgccg cgcccgccaa ggagatatcc
      481 gagtacgcgc ctaagattct ctccgtcaag atcgacgtgg acaagatcgg cgccatcatc
      541 ggcccgggcg gtaagaacat caaggccctt tgcgagcagt acggcgtcag gatcaacgtc
      601 gacgacgacg gcaccgtgac catcttcggc gccaaccaga aggcgtcgct cgaggccaag
      661 gcagccgtgc tcggcatcgc ccaggatccc gaggtcggca ttgtctacca gggcaccgtc
      721 aagcgcgtga tggacttcgg cgccttcgta gagatcttgc ccggcaagga aggcctcgtc
      781 catatctcca ggctgtcgcg gaccagaatc aacgccgcca gcgacgtcgt caaggaaagg
      841 caggtcatac cggtcaggct gcttgaggtc gacaagctcg gccgcctgaa cttgtcgtat
      901 atcgacgcca tggacgacgc cggccagaag aagtaggata catcgggcca gtcccgagcg
      961 cgtatagaag gccggccgcg agtccggcct tcttcattca taagacgcga ggatcgcaga
     1021 tgctccgtga cgatactgga atcatcttta accgcgagct ggtccccggc ttcaggcttc
     1081 tggccgatga gatgccgggg gcgacgggcg tagccatcgg cctttggttc cccgtcggct
     1141 cggccctcga gcgggacggg gagcgtgggc tgtcgcactt cgtcgagcac atggtgttca
     1201 agggcgcggg cgaccgggac gcagccgagc tttcgcgctc gatagaccgg gtcggtggct
     1261 acctgaacgc cttcaccgag cgcgaagccg tctgtctgca ctgcctggtg ccggccaggc
     1321 atgcggagct ggccgtcgat atactcatgg acatggccta taggccgcgg ctgtcggctg
     1381 tagaattcga gcgcgagaag gacgtcatcg tcaacgagat cctggctgcc gaggacgacc
     1441 tcgaggaggc tgcccaggac gagttcttcg cgttggccta cgacggacat ccggctgcta
     1501 ggcgcatagc cggcgaggtc ggcgatatcc gcgaggcggt tttctcctcg ctggccgagt
     1561 tccacgacga gcgcttctcc agggggccgg taacgttgac ggtctccggg gccgttcagg
     1621 ccgaggcctt ggccgaacgc gtcgttgcgg cgttaccggc gcagaggcgc tcggcgagcg
     1681 gcctggcgga agcctgggat cctggcgagc ctcggttcgt ccgctgccgg cgcatggtca
     1741 agg
//
LOCUS       DAOOLN010000396         1742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_87857_length_1742_cov_3.014819, whole genome shotgun sequence.
ACCESSION   DAOOLN010000396 DAOOLN010000000
VERSION     DAOOLN010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87857_length_1742_cov_3.014819"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PLW80_05765"
     CDS             <1..282
                     /locus_tag="PLW80_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66047.1"
                     /translation="ATFEQLVGFLARYIEDAKRVEDAIRKQYAPRLKAKEQEMSARMG
                     RPVRIDPMQDPDFAAFYKQNVGQMRDQYQAALDQAKADLAKLCGIHKDD"
     gene            complement(406..>1742)
                     /gene="mnmG"
                     /locus_tag="PLW80_05770"
     CDS             complement(406..>1742)
                     /gene="mnmG"
                     /locus_tag="PLW80_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPB66048.1"
                     /translation="AAAVGLGSWLRAAGFPVGRMKTGTPARVKASSVDLGSLELQPGD
                     SERRPFSFMNDEARGPELPCHITYTNERTHAIIRANIARSPLYSGDITGKGPRYCPSI
                     EDKVVKFPDRDRHQIFVEPEGLYTDELYLNGLSSSMPEDVQEAFIRTMAGFEGAYVVR
                     PGYAVEYDYVDPVDLYASLESKRLRGLFMAGQTNGTSGYEEAAAQGLVAGVNAALSTA
                     GEPPLVLSRADAYIGVLIDDIVTLGAKEPYRMFTSRAERRLALRHDTADRRLTPIAMR
                     VGLASKERAERFERKVEGMDAVRELLRARRVSQADAERDGAFSPHVGEGLARALADPH
                     IAELDVETIEPAVSAYPRDWVQGVILDLRYEGYVAKEERLSARLGKLDAARLSPDFDY
                     AAVPGLSAESVEKLSATRPLSLGQASRIPGVRPSDVALLAVRLATGARPSRA"
BASE COUNT          281 a          596 c          594 g          271 t
ORIGIN      
        1 gccaccttcg agcagctcgt cggcttcctg gcgcggtaca tagaggacgc caagcgcgtc
       61 gaggatgcga tccgcaagca gtacgccccg aggctcaagg ccaaggagca ggagatgtcg
      121 gccaggatgg gccggcccgt acgcatcgac ccgatgcagg acccggactt cgccgcgttc
      181 tacaagcaga acgtcggtca gatgcgcgac cagtaccagg ccgcgctcga ccaggcgaag
      241 gccgacctcg ccaagctgtg cggtattcat aaggacgact aggaccgcta cccccgggcg
      301 cgtccgcgcc cctcgggcgc cccgcgcccg agaccctgcg taggccgtcg cttcggcccg
      361 gccgccggcc tcaggtgcga ccgccccgca tcccggttgc cggtctcagg cacgactcgg
      421 gcgagccccg gtcgccagcc tgaccgccag tagcgccaca tcgctcggcc tgacgcccgg
      481 tatacgcgag gcttgaccga gcgatagcgg cctggtagcg gacagtttct cgaccgactc
      541 ggcggacagg ccgggtacgg cggcgtagtc gaagtcgggt gacaacctgg cggcgtcgag
      601 cttgccgagt ctagccgata agcgctcttc cttggccacg tagccctcat agcgcaggtc
      661 caggatcacg ccctgcaccc aatcgcgcgg ataggccgat acagccggct ctatcgtctc
      721 cacgtctagc tccgcgatat gcggatcggc cagggctctg gccaggccct ccccgacgtg
      781 cggcgagaag gctccgtcgc gttcggcgtc agcctgcgac acccggcgcg cgcgcaggag
      841 ttcgcgcacg gcgtccatgc cctcgacctt gcgctcgaaa cgctcagcgc gctccttgga
      901 agccagaccg acgcgcatgg ctatcggggt caggcgacgg tcggcggtgt cgtggcgcag
      961 ggccaggcga cgctcggccc ggctcgtgaa catgcggtac ggctccttgg cgccaagggt
     1021 cacgatgtcg tcgatcagca caccgatgta cgcgtcggcc ctggacagca cgagcggagg
     1081 ctcgcctgcc gtcgacagcg cggcgttgac gccggccacg aggccctggg ccgcggcctc
     1141 ctcgtagccg gaggtgccgt tcgtctggcc ggccatgaac aggccgcgta gacgcttcga
     1201 ctcgaggctc gcgtacaggt cgacggggtc gacgtagtcg tactcgaccg cgtagcccgg
     1261 cctgaccacg taggcgccct cgaagccggc catcgtcctg atgaaggcct cctgcacgtc
     1321 ctcgggcatc gatgacgaca ggccgttcag gtacagctcg tcggtataga ggccctccgg
     1381 ctccacgaag atctggtggc ggtcacgatc agggaacttg accaccttgt cctcgatgct
     1441 cgggcagtag cgcggcccct tgccggtgat gtcgccggag tacaaaggag atcgggcgat
     1501 gttggcgcgt atgatcgcgt gggtacgctc attcgtgtag gtgatatggc acggtagttc
     1561 cggcccgcgc gcttcgtcat tcatgaacga gaacgggcgc cgctcggagt caccgggctg
     1621 gagttcgagg gagcccaggt ctacgctgga cgccttgacg cgagccggcg tgccggtctt
     1681 catgcggccg accgggaagc cggcggcccg tagccaggaa ccgagtccga cggcggcggc
     1741 tt
//
LOCUS       DAOOLN010000397         1742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_87860_length_1742_cov_2.928275, whole genome shotgun sequence.
ACCESSION   DAOOLN010000397 DAOOLN010000000
VERSION     DAOOLN010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87860_length_1742_cov_2.928275"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..1145)
                     /gene="hydE"
                     /locus_tag="PLW80_05775"
     CDS             complement(39..1145)
                     /gene="hydE"
                     /locus_tag="PLW80_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HPB66049.1"
                     /translation="MALGRLSERAFAVVAGAWPVEPPAPDDVRLLLDSADEATADALK
                     AAASRARDSGLGPRLVARALIEISSYCGNSCRYCGLNACNRLARRYSLSKDEIVESAS
                     RAFRAGLRTIVLQSGEDGRDTAELSAIIGAVKRAGFRPDAVDGAGYPAVTLSLGERPE
                     ADYAAWKEAGADRYLMRMETGDRELYAALHDGRGLDSRLACHAALRRLGYQLGSGIMV
                     GLPGQSVSGIALDLAAMAGGRYAMLGIGPFIPHPDTPLGGATPGDMGLALRATALARI
                     CSPWSWIPATTAMASQERDYRVDALLYGANVVMPNFSPAYARGAYSIYPGKRCAAESD
                     DEQRPEHAADSLRRLADAAGLVVDPGRADDAWPT"
     gene            complement(1160..>1742)
                     /locus_tag="PLW80_05780"
     CDS             complement(1160..>1742)
                     /locus_tag="PLW80_05780"
                     /inference="COORDINATES: protein motif:HMM:NF037048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HPB66050.1"
                     /translation="ALAALSRAPDLVVCDSQVIGAVHRDTPPGVALTTFSILFSRVKG
                     DMEALAAGCATLSALSPGDRVLVAEACTHHPSFDDIGRVKLPRWIREHAGSAIDIDHS
                     AGGDYPADLGGYRLVVHCGACTLTRKEGIVRIERAREAGVAITNYGMAISLMHGALER
                     ALEPFPSALAAYRDAALRDAALRDAAGDRAATL"
BASE COUNT          254 a          588 c          649 g          251 t
ORIGIN      
        1 cggcggcggt tccggcaagc tcaggtgcta caggatgctc aagtaggcca cgcgtcgtcg
       61 gccctgccag ggtcgacgac gaggccggcg gcgtcggcga ggcgcctgag gctgtccgcc
      121 gcgtgttcag gccgctgctc gtcgtccgac tcggcggcgc agcgctttcc gggatagatg
      181 gagtaggcgc cgcgcgcgta ggcaggcgag aagttcggca tgacgacgtt ggcgccgtag
      241 aggagcgcgt cgacccggta gtcgcgctcc tgactggcca tcgcggtcgt ggcgggtatc
      301 cacgaccatg gcgagcagat gcgcgcgagc gcggtggccc gcagcgcgag gcccatgtcg
      361 cccggcgtcg cgcctccgag cggcgtgtcc ggatgcggta tgaagggccc gatgccgagc
      421 atcgcgtacc ggccgcccgc catggccgcc aggtcaagag cgataccgga tacgctctgc
      481 cccggcaggc ctaccatgat gcccgatcct agctggtaac cgaggcgcct gagcgccgcg
      541 tggcaggcca gccgggaatc gaggccgcgg ccgtcgtgaa gggccgcgta cagctccctg
      601 tcgcccgtct ccatgcgcat aaggtaacgg tcggcgccgg cctccttcca ggcggcgtaa
      661 tccgcctccg gccgttcgcc gagcgacagc gtgacggcgg ggtagccggc gccgtcgacg
      721 gcatcagggc ggaagccggc ccgcttgacg gcgcctatga tggcggacag ttcggccgtg
      781 tcgcggccgt cctcgcctga ttggagcacg atcgtgcgga ggcccgccct gaacgcgcgg
      841 gaggccgact cgacgatctc gtccttcgac aggctgtagc ggcgcgccag gcggttgcag
      901 gcgttgaggc cgcagtaacg gcagctattg ccgcaatagc tcgatatctc gatgagggcc
      961 ctggcgacga gccgggggcc gagccccgag tcgcgggcgc ggctggcggc cgccttgagc
     1021 gcgtcggccg tcgcctcgtc cgcgctatcg aggagaagcc gcacgtcgtc cggtgccggc
     1081 ggctcgaccg gccaggctcc cgccacgact gcgaaagcgc gctcggacag cctgccgaga
     1141 gccatgcccg cctcgcgcgt catagcgtag cggctctgtc cccggccgcg tcgcgcagag
     1201 ccgcgtcgcg cagagccgcg tcgcggtaag ccgcgagcgc gctcgggaag ggctcgagcg
     1261 cgcgctcgag cgcgccgtgc atcagcgata tggccatgcc gtagttcgtt atggctacgc
     1321 ctgcctcgcg ggcgcgctcg atgcggacga tgccctcctt gcgcgtcagg gtgcaggcgc
     1381 cgcaatggac gacgagccta tatccgccga ggtcggctgg atagtcgcca ccggcggagt
     1441 ggtcgatatc gatagctgaa ccggcgtgct cccgtatcca gcgcggtagc ttgaccctgc
     1501 ctatgtcgtc gaagctggga tggtgggtac aggcctcggc gacgagcacg cggtcgcccg
     1561 gggacagagc cgagagggta gcgcatccgg cggccagggc ctccatgtcg cccttgaccc
     1621 gcgagaagag tatcgagaag gtggtcagcg ctacgccggg cggcgtgtcg cgatggacgg
     1681 cgcctatcac ctgcgagtcg cagacgacga ggtcgggcgc cctggaaagg gccgccagcg
     1741 cc
//
LOCUS       DAOOLN010000398         1741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_87941_length_1741_cov_2.870107, whole genome shotgun sequence.
ACCESSION   DAOOLN010000398 DAOOLN010000000
VERSION     DAOOLN010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87941_length_1741_cov_2.870107"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PLW80_05785"
     CDS             complement(<1..422)
                     /locus_tag="PLW80_05785"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HPB66051.1"
                     /translation="MHRRSITNDAIKGGLVLAMIAYHACYVGAMFDLARLELFSGFWW
                     MFPRVIAAGFVAVSGWNLAGKRAAGSGFGSFAKRAGKLGLVALAISAATRPVLGEGFV
                     FFGVIHLLALSSLIAYPLLGRPALAIVAGAAALVAGVLL"
     gene            503..1210
                     /locus_tag="PLW80_05790"
     CDS             503..1210
                     /locus_tag="PLW80_05790"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPB66052.1"
                     /translation="METEIRIDQAFFSDLERPASFYILRHGETVANAESRIQGRSEYP
                     LNENGRAQAADLARHLEGAGVRRLLYSPLSRARETAAIVAAALGLDGSELAAEPALVE
                     LDTGRFSGLTLTEIRERFPAEHEAFGYCSWEAVPDAERAEELYRRASLAWSRLKAEAK
                     ASGGNVAAVSHGGMIQWLVRATFGCRSWMPLLSTGNCGVFELFVDPVGGGRPAYLQWK
                     ELNRLPGGTAGRTPPVF"
     gene            complement(1207..>1741)
                     /locus_tag="PLW80_05795"
     CDS             complement(1207..>1741)
                     /locus_tag="PLW80_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HPB66053.1"
                     /translation="PAHRLDRRTSGAVAFAKSKHSLAALSEAFRERRVTKTYWAVTER
                     APEPAAGTLRHRLVHDKRRNQAKAVPANGDRGGDVAVLTYELVGRSDRYFLLAVRPLS
                     GKTHQIRAQLSAAGFPVRGDLKYGSRRSTRNGLIMLHARELSFEMPDRRRVYVTADPP
                     PDEPLWEAFVEDPQNVN"
BASE COUNT          259 a          588 c          625 g          269 t
ORIGIN      
        1 agcaggactc ccgctaccag cgccgcggcg cccgctacga tggcgagggc cggcctcccc
       61 aggagggggt aggcgatcag cgacgacagc gccagtagat ggatcacccc gaagaacacg
      121 aagccctcgc cgaggacggg ccgggtcgcg gcgctgatcg ccagcgcgac aaggccgagc
      181 ttgcccgccc gcttcgcgaa ggagccgaag cctgagccgg cggcccgctt gccggccagg
      241 ttccagccgg ataccgccac gaagccggct gcgattaccc gtgggaacat ccaccagaag
      301 ccggagaata gctcgagccg ggccaggtcg aacatcgccc cgacgtagca ggcgtggtag
      361 gctatcatgg ctaggacgag gccgcctttg atggcgtcgt tggtgatcga acgtcggtgc
      421 atcggggcag tatacgcctc gccgcggcgg gtggtaaagc gcgcttgagc cgcccgtcgt
      481 cccggtgcta tagtcgccgg cgatggaaac cgaaatccgt atcgaccagg ccttcttttc
      541 cgacctcgag cggcccgcga gcttctatat cctcaggcat ggcgagacgg tcgccaacgc
      601 cgagagccgc atccagggcc gttcggagta ccctctgaac gagaacgggc gagcccaggc
      661 cgcggaccta gccaggcacc tcgagggagc gggcgtgcgc cgcctgctgt actcgccgct
      721 ctcacgcgcc cgcgagacgg cggccatcgt cgcggcggcc ctcggcctcg acggctcgga
      781 actcgcggct gagcctgccc tcgtcgagct ggacacgggg cgtttctccg ggcttacctt
      841 gacggagata cgggagcgtt tcccggcgga gcacgaggcc ttcgggtact gcagttggga
      901 ggccgtgccg gacgccgaac gcgcggagga gctctaccga cgcgcgtcgc tcgcctggtc
      961 caggctcaag gccgaggcta aggcctcggg cggcaacgtg gcggccgtgt cgcacggcgg
     1021 catgatccag tggctcgtcc gcgcaacctt cggctgccgt agctggatgc cgcttctgtc
     1081 cacgggcaac tgcggcgtct tcgagctgtt cgtcgacccg gtgggcggag gccgccccgc
     1141 ctatctgcag tggaaggaac tgaatcggct acccggcggt acggcgggcc gtacgccgcc
     1201 ggtgttctag ttcacgttct gcgggtcctc gacgaaggcc tcccagaggg gctcgtccgg
     1261 gggcgggtcg gccgtgacgt atacgcggcg gcggtcaggc atctcgaaac tcagctcgcg
     1321 ggcgtgcagc ataatcaggc cgttccgcgt cgatcgcctg gagccgtact tcaggtcgcc
     1381 gcgcaccggg aagcctgccg cggacagctg ggcccgtatc tggtgcgtct tgcccgacag
     1441 gggccgtacc gccagcagaa agtaccggtc gctccggccg accagctcgt aggtcagtac
     1501 cgctacatcg cctccgcggt cgccgttggc cggtacggcc ttggcctggt tgcgtcgctt
     1561 gtcgtggacc agccggtgcc gcagtgtgcc ggcggccggc tcgggggcgc gctcggtgac
     1621 ggcccagtag gtcttggtca cgcggcgctc gcggaaggcc tcggataggg ccgccaggct
     1681 gtgcttcgac ttggcgaagg cgaccgcgcc gctcgtgcgt cggtcgagcc tgtgggccgg
     1741 c
//
LOCUS       DAOOLN010000399         1739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_88072_length_1739_cov_3.590855, whole genome shotgun sequence.
ACCESSION   DAOOLN010000399 DAOOLN010000000
VERSION     DAOOLN010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88072_length_1739_cov_3.590855"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PLW80_05800"
     CDS             complement(<1..621)
                     /locus_tag="PLW80_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66054.1"
                     /translation="MTGRSTRYAHAPWPAALPAALAAIAVLFSACSGGAPRPEATPAP
                     EPGFQLPSGGTLVLAVDASGGLYPLPPPETGSVPAEPVSEPFPIAVSAATIQPIVGGA
                     VVAINRKGLSRLTIRRSAPDVAASVLIEPIDGAAAEFDGRTVAPSWGSGGDAVFLLYR
                     HPVYELAERRSPASVTIAATLDRARIVRPGLGDDAFAAYPRAPGEWL"
     gene            complement(715..>1739)
                     /locus_tag="PLW80_05805"
     CDS             complement(715..>1739)
                     /locus_tag="PLW80_05805"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HPB66055.1"
                     /translation="LARLFLRDGKKDEALKATETMLEVYRAFAEEYLCLPVVAGRKSD
                     AEKFAGAVATYSIEAMMQDKKALQAGTSHFLGQNFAKAFEVQYQTREGGLDYVWATSW
                     GVSTRLLGAIIMTHSDDKGLVLPPSLASEEIVVVPIFKTDNKAAVLEYAGKVVDALKA
                     DGRRVVFDTDDGSSPGWKFAEWEMRGVPIRIEAGPKDAANGTVVMVRRDTGEKTFVKV
                     DEVPAKARETLAAMQKDLLEKARAFQDANTKDVADRDELVRFFGKEKGKLDADGELHE
                     GTVEAPGGFARGLWCGGRDCEAQLKADLKVTIRNLPFGQQDGCDGACCLCGAPAKHRA
                     IFARSY"
BASE COUNT          261 a          582 c          613 g          283 t
ORIGIN      
        1 cagccactcg ccaggagcgc ggggatacgc ggcgaaggcg tcgtcgccga ggcccggtcg
       61 aacgatcctc gctcgatcaa gcgtggcggc tatagtcacc gaagcgggcg accgccgctc
      121 ggccagctcg tagaccgggt gccggtagag gaggaagacg gcgtcaccgc ccgagcccca
      181 ggacggtgcg acggttcgcc cgtcgaattc ggccgccgcg ccgtcgatcg gctcgatcag
      241 gacactagcg gcgacgtcgg gggccgagcg ccggatcgtc aggcgactga gacccttccg
      301 gtttatggcg acgacggcgc cgccgacgat cggctgtatc gtcgccgcgg agaccgcgat
      361 cgggaagggt tccgagaccg gctcggcagg aaccgaaccg gtctcgggcg ggggtagcgg
      421 atacaagcca cccgaagcgt ccaccgccaa cacgagcgtc ccgccggagg gtagctggaa
      481 gccgggctcg ggtgccggag tagcctcggg ccgcggcgct ccgcccgagc aggcggagaa
      541 tagcacggct attgcagcga gggccgcggg tagcgcggcg ggccatggtg cgtgggcata
      601 gcgagtcgat cgtccggtca tggcgccagt ctagcaggag aacggcgatg ccggaagacg
      661 aaaacgccgc cccgcgatgg gcgtggggcg gcggcttggt ccgtagggcc ggtctcagta
      721 cgaccgtgcg aagattgcgc ggtgcttggc cggcgcgccg cagaggcagc aggcgccgtc
      781 gcaaccgtcc tgctggccga acggcaggtt gcgtatcgtc accttcaggt cggccttgag
      841 ctgggcctcg cagtctctgc cgccgcacca taggccgcgg gcgaatccgc cgggagcctc
      901 gacggtgcct tcgtgcagct cgccgtcggc gtcgagcttg cctttctcct tgccgaagaa
      961 ccgtacgagc tcgtcgcgat cggcgacgtc cttcgtattg gcgtcctgga aggccctggc
     1021 cttctccagg aggtccttct gcatcgcggc gagggtctcg cgcgcctttg ccggcacttc
     1081 atcgaccttg acgaaggtct tctccccggt gtcgcggcgc accatcacga cggtgccgtt
     1141 ggccgcgtcc ttggggccgg cctcgatacg gataggcacg ccgcgcatct cccactcggc
     1201 gaacttccag ccggggctcg agccatcgtc ggtatcgaag accacgcgtc ggccgtcggc
     1261 cttgagcgcg tcgacgacct tgcccgcgta ttcgagcacg gcggccttgt tgtcggtctt
     1321 gaagatcggc acgacgacga tctcctcgct ggccagcgac ggcggcagca cgaggccctt
     1381 gtcgtcggag tgcgtcatga tgatggcgcc gagcagcctc gtcgacacgc cccagctcgt
     1441 cgcccagacg tagtcgaggc cgccttcgcg ggtctggtac tgtacctcga aggccttggc
     1501 gaaattctgg ccgaggaagt ggctggtgcc ggcctgcagg gctttcttgt cctgcatcat
     1561 cgcctcgatg gaatacgtgg cgaccgcgcc ggcgaatttc tccgcgtcgc tcttgcgacc
     1621 ggccacgacc ggcaggcaga ggtactcctc ggcgaaggcc cggtagacct cgagcatcgt
     1681 ctcggtcgcc ttgagcgcct cgtccttctt cccgtcgcgc aggaagagcc gggccagct
//
LOCUS       DAOOLN010000400         1738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_88166_length_1738_cov_3.031491, whole genome shotgun sequence.
ACCESSION   DAOOLN010000400 DAOOLN010000000
VERSION     DAOOLN010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88166_length_1738_cov_3.031491"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PLW80_05810"
     CDS             complement(<1..243)
                     /locus_tag="PLW80_05810"
                     /inference="COORDINATES: protein motif:HMM:NF027796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HPB66056.1"
                     /translation="MTDPRNSPFMYAIGAFVALFVTVQSLVFMIRAYREGKRLGLSAA
                     TMRKAIVGSASFSVVPSIAILLAVLTLSGALGLPLPW"
     gene            complement(430..>1738)
                     /locus_tag="PLW80_05815"
     CDS             complement(430..>1738)
                     /locus_tag="PLW80_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB66057.1"
                     /translation="AESGASVPAAMLGDWDGELVAGAQKLALRLHIAAGGVSLDVPAQ
                     RVAGAKASSVIFADGLLVAEFDAFRASVELRAAEGGGLEAIWAQSGARLPLTFARAGD
                     GSAAAVPAAPAVPARRGESVRFKSGQAGVELAGTLRLPDGKGPFPCLILVSGSGAQDR
                     DEAIGPITPFRDIAEGMAARGWATLRYDDRGVGESGGTFSGSTSEDFALDAVGAYRFA
                     SADGRIDARRVALAGHSEGGYIIAIAENLLGGVYGLVSIAGPGVGGYEILLDQSRDIL
                     AGMGAPAEAIESSQAINRALYDMILESDEFPLEAVKAELSKAGLPPDQLEAQLPMLRD
                     PWFKAFLRSNPAGYYSKVRSPVLAINGSKDSQVRAGKNLAAIEAAVKRAGVPVKAVAM
                     DGLNHLFQQANTGMPDEYMGLPPAFSPEAIALMADWLDRLGSR"
BASE COUNT          279 a          596 c          601 g          262 t
ORIGIN      
        1 ccacggcagg ggcaggccga gggcgccgga cagagtcagc acggcgagca ggatggcgat
       61 ggacggaacg acggagaagc tggccgagcc gacgatggcc ttgcgcatcg tcgccgcgct
      121 caggccgagg cgcttaccct ccctgtaggc cctgatcatg aagaccaggc tctggaccgt
      181 gacgaacagg gcgacgaagg ccccgatcgc gtacatgaac ggactgttcc gcgggtcggt
      241 catggggacg actccttggt tcgattgttt tcagcgatag aaactgtgat cgattaaacc
      301 gtcgcatccg gccgctgtca acgaaaaagc gcccggacgg cgtcggccat ccgggcgctc
      361 gaacaaagat gcgtcgcgcg atgcgtcgcg cgttcaaagg cgcgcggatc atgcgccccg
      421 gcgttttacc tagcgcgacc cgagcctgtc gagccaatcg gccatcagcg ctatcgcctc
      481 gggcgagaaa gccggcggaa ggcccatgta ctcgtcgggc atgccggtat tcgcctgctg
      541 gaacaggtgg ttaagcccgt ccatcgcgac ggccttaacc ggcacgccgg cccgcttgac
      601 ggccgcctcg atggccgcca ggttcttgcc ggcgcgcacc tgcgagtcct tggagccgtt
      661 gatcgcgagg accgggctcc tgaccttgga gtagtatccg gccgggttcg agcgtaggaa
      721 ggccttgaac catgggtcgc gaagcatcgg cagctgggcc tcgagctgat ccgggggcag
      781 gcccgccttg gacagctcgg ccttcaccgc ctcgagcggg aactcgtccg actcgagtat
      841 catatcgtag agggcccggt tgatggcctg ggagctctcg atcgcctcgg cgggggcgcc
      901 catgccggcg agtatgtccc tcgactggtc gaggagtatc tcgtagccgc cgacgccggg
      961 gccggctatc gatacgaggc cgtatacgcc gccgagcagg ttctcggcta ttgctatgat
     1021 gtaaccgccc tcgctgtgtc cggcgagcgc cacgcgcctc gcgtcgatgc ggccatcggc
     1081 cgacgcgaag cgataggcgc ctaccgcgtc gagcgcgaaa tcctccgagg tggagcccga
     1141 gaaggtcccg cccgactcgc cgacgccgcg gtcgtcgtag cgcagcgtgg cccagcccct
     1201 ggcggccatg ccctcggcaa tgtcgcggaa cggcgtgatc gggcctatcg cctcgtcgcg
     1261 gtcctgggct cccgagccgc tgaccagtat caggcagggg aaggggccct tcccgtccgg
     1321 gagcctgagc gtgcccgcca gctcgacgcc ggcctggccg ctcttgaagc ggacgctctc
     1381 gccgcgtcgt gccgggaccg caggggccgc cggaaccgcg gccgccgatc cgtcgccggc
     1441 ccgagcgaag gtcaacggta agcgagcgcc gctctgggcc cagatcgcct cgagcccgcc
     1501 gccctcggca gcgcgcagtt ccaccgaagc cctgaacgcg tcgaactcgg cgacgagtag
     1561 cccgtcggcg aagatgaccg aggaagcctt ggccccggcg acgcgttggg ccggtacgtc
     1621 gagcgagacg ccgccggccg ctatatggag cctgagcgcc agcttctggg cgccggcgac
     1681 gagctccccg tcccagtcgc ccagcatggc tgccggtacc gaggcaccgc tctccgcg
//
LOCUS       DAOOLN010000401         1736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_88349_length_1736_cov_2.187983, whole genome shotgun sequence.
ACCESSION   DAOOLN010000401 DAOOLN010000000
VERSION     DAOOLN010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88349_length_1736_cov_2.187983"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLW80_05820"
     CDS             <1..334
                     /locus_tag="PLW80_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66058.1"
                     /translation="ADSPYYPRAVSLYRQLGPGAWAWTCSPSVKAQASSETTVISADY
                     PVGSSHYMALYGVKPYVKIQLYGLDYNMDASFENYNASGYFYKKAAGAMYLKMRHKVQ
                     GEEIRLFY"
     gene            547..687
                     /locus_tag="PLW80_05825"
     CDS             547..687
                     /locus_tag="PLW80_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66059.1"
                     /translation="MAEAAAPDGAGRSGYIVAIRREDRERLEETMSGLRVRWIGETIR
                     WR"
     gene            744..>1736
                     /gene="dgcA"
                     /locus_tag="PLW80_05830"
     CDS             744..>1736
                     /gene="dgcA"
                     /locus_tag="PLW80_05830"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcA"
                     /protein_id="HPB66060.1"
                     /translation="MGTSDAARIREEYEKQIFDLKQLLEVSKSLNSTLDYGILIESIL
                     YTIMGQMKVLKAALYAKKGIDSTRFNLHRNYKGFEIEHGRDYSIPEDAPAMRAFARET
                     RCWTMAELRERFGDLGGLEPIEPMAPTLIVPLKAKGVVNGLILVGEQIVSEAFSDYEK
                     EHLLNIAILASIAINNAFLFEMTTTDMMTKLRMKHYFYTVLLEKMEESAFSEKPLSVI
                     MADIDSFKRFNDTYGHSCGDEVLKSVATILRNNLRGQDLAARWGGEEFLLLLRNMDAD
                     EAVAVAERIRHIIAATPFACGDETFQVTLTFGISCWNDPHTTIDYCVDCSDQALY"
BASE COUNT          353 a          550 c          542 g          291 t
ORIGIN      
        1 cgccgactcg ccgtactacc cgcgggccgt cagcctgtac agacagctcg gccccggcgc
       61 ctgggcctgg acctgttcgc cgtcggtcaa ggcgcaggcc agctccgaga ccacggtgat
      121 cagcgccgac taccctgtcg gctcgtcgca ctatatggcg ctctacggcg tgaagcccta
      181 cgtgaagatc cagctctacg ggctcgacta caacatggat gccagcttcg agaactacaa
      241 cgcctcaggc tatttctata agaaggcggc cggggccatg tacctcaaga tgcggcacaa
      301 ggtccaggga gaagagatac ggctattcta ctagggggcc gtcccctccc ctcgaccttc
      361 cagacggccg gcgagctcgt ctacctcgtc gaaggagctc ggtccgaggc cgtcgaagcg
      421 gccatagcca gggcggccgc cggccgcgtt gcccgggccg ccagggcctg gtcaggctcg
      481 cccgacgccg cgttgtcggc ggcgtcgggc tgccttggcg tcgaggcctg aaccgacgcg
      541 ctcgagttgg ccgaggccgc ggcgccggat ggcgccggac ggagcgggta tatcgtcgcc
      601 atcaggcggg aagaccgcga gcgcctcgag gagaccatga gcggcctgcg ggtgcgctgg
      661 ataggcgaga caatccggtg gcgctaatca tgatgccgac gtatactatg cggaaagcca
      721 ctattcagaa tgtgagacga tagatgggaa ccagcgacgc cgcgcgtata cgagaagagt
      781 acgaaaagca gatattcgac ctgaagcagc tcctcgaggt atctaaaagc ctgaattcca
      841 ccctcgacta cggcatcctg atagagtcga ttctctacac gatcatgggc cagatgaagg
      901 tgctcaaggc cgccctgtac gccaagaaag gcatagactc gacccgcttc aacctgcacc
      961 gtaactataa gggcttcgag atcgagcacg ggcgcgacta cagcatcccg gaggacgccc
     1021 cggcgatgcg cgccttcgcg cgggagaccc gctgttggac catggccgag ctgcgggagc
     1081 gcttcggcga ccttggcggc ctcgagccga tcgagccgat ggcgccgacc ctgatcgtcc
     1141 cgctcaaggc caagggcgtc gtcaacggcc tgatactcgt cggggagcag atcgtcagcg
     1201 aggccttctc ggattacgag aaggagcacc tcctcaatat cgccatactg gcgtcgatag
     1261 ccatcaacaa cgccttcctg ttcgagatga cgacgaccga catgatgacg aagctcagga
     1321 tgaagcacta tttctatacg gtgctgctcg agaagatgga ggagagcgcc ttctcggaga
     1381 agccgctgtc ggtgatcatg gccgatatcg actcgttcaa gcgcttcaac gacacctatg
     1441 ggcatagctg cggcgacgag gtgctcaaat ccgtggcaac gatcctccgc aacaacctgc
     1501 gcggccagga cctggcggcc cgctggggcg gcgaggaatt cctcctgctg ctcaggaaca
     1561 tggatgcgga cgaggcggtg gcggtggccg aacgcatccg gcacatcatc gccgccacgc
     1621 cctttgcctg cggcgacgag accttccagg tcaccctgac ctttggcatc agctgctgga
     1681 acgatccgca cacgaccatc gattactgtg tcgattgctc ggaccaggcc ctgtac
//
LOCUS       DAOOLN010000402         1735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_88403_length_1735_cov_2.858929, whole genome shotgun sequence.
ACCESSION   DAOOLN010000402 DAOOLN010000000
VERSION     DAOOLN010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88403_length_1735_cov_2.858929"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="PLW80_05835"
     CDS             complement(<1..923)
                     /locus_tag="PLW80_05835"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HPB66061.1"
                     /translation="MVILTLNCGSSSVKYQVYDWDEKNVLASGIVERVGIEGSIINHK
                     APGVADVSLEHECPNHVVAVELILKTITDPQHGVVRSMKDIGAVGHRMVHGGMKFARS
                     VLITDESLATFKELIDLAPLHNPANITGVEAAKAVLPNVPHCAVMDTAWHQTMPSTSY
                     TYALPHEWQDKYMVRRYGFHGTSFLYTAKRASVLLGKDPFDSNVIIAHIGNGSSINAV
                     KDGCSYDTSMGMTPLEGLVMGTRSGDIDPGIVFHMMKKAGLSAAEVEKALNKESGVLG
                     ITGRWSDRRDIENAAAEGDRIAIVAQELEGYR"
     gene            complement(1006..>1735)
                     /locus_tag="PLW80_05840"
     CDS             complement(1006..>1735)
                     /locus_tag="PLW80_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66062.1"
                     /translation="LEQVGPAVANRREQPESETYLAYCFADVGSAPEDFIKENELAFT
                     RLLAGEPADYPIHGSQVRKTMSNPFSYSDRDLAVFDMDRCLIVDSDRDYEDVLLICEL
                     ANYQYLELRALDKWLDKWLDEAEDDVNLIARKGAGRGLKTGTLRKKLGKIQALRLDAL
                     FILENLENSSKIIGDYFLGQVYEHVCGIFNTSGWTRSVERRLDALQDIYEIVKTENSE
                     RTMLLLEIVFIVVCIIPIIQFAFG"
BASE COUNT          295 a          496 c          591 g          353 t
ORIGIN      
        1 cggtagccct cgagctcctg ggcgacgatg gcgatcctat cgccctcggc ggcggcgttc
       61 tcgatgtcgc gcctgtcgga ccagcggccg gtgatgccga gcacgccgga ttccttattc
      121 agcgccttct cgacctcggc tgcggacagg ccggccttct tcatcatgtg gaagacgatg
      181 ccggggtcga tatcgccgga acgggtgccc atgaccaggc cctcgagcgg ggtcatgccc
      241 atcgaggtgt cgtaggagca gccgtccttg acggcgttga tcgatgagcc gttgccgatg
      301 tgcgcgatga tgacgttcga gtcgaaggga tccttgccga gcagcaccga ggcgcgcttg
      361 gccgtataga ggaagctggt gccgtggaag ccgtagcgcc gcaccatgta cttgtcctgc
      421 cattcgtggg gcaatgcgta ggtgtagctg gtgctgggca tggtctggtg ccaggcggtg
      481 tccatgacgg cgcagtgggg cacgttcgga agcacggcct tggccgcctc gacgccggtg
      541 atgttggcgg gattatggag cggtgccagg tcgatgagtt ccttgaaggt agccagggac
      601 tcatcggtga tcagcaccga gcgggcgaac ttcatgccgc cgtggaccat gcggtggccg
      661 accgcgccga tgtccttcat cgaccgtacg acgccgtgct gcgggtcggt gatggtcttg
      721 agtatcagct cgactgcgac gacgtggttg gggcactcgt gctcgaggct cacgtcggcg
      781 acgcctggcg ccttgtggtt gatgatcgag ccttcgatgc cgacgcgctc gacgatgccg
      841 ctcgcgagta cgttcttctc gtcccagtcg tagacctggt acttgacgga cgaactgccg
      901 cagttgaggg taagtatgac catgaggttc tccttagcgt ggttatcagg agagactgta
      961 ccagagacgc gagcgcgggt ctacagagcg cgggacggac gctggctagc cgaacgcgaa
     1021 ctggatgata ggaatgatac agacgacgat gaacacgatc tcgagcagca gcatggtgcg
     1081 ttccgagttc tcggtcttga cgatctcgta gatatcctgg agcgcgtcga ggcgccgctc
     1141 gaccgagcgg gtccaccccg aggtgttgaa gatgccgcag acatgctcgt agacctggcc
     1201 caggaaatag tcgcctatga tcttcgaact gttctcgagg ttctcgagga tgaacagcgc
     1261 gtcgaggcgc agagcctgga tcttgccgag cttcttgcgc agggtaccgg tcttgagccc
     1321 gcgacccgct cccttcctgg ctatcaggtt gacgtcgtcc tcggcctcgt cgagccattt
     1381 atcgagccac ttatccaggg cgcgcagctc gaggtactgg tagttggcca gctcgcagat
     1441 cagcagcacg tcctcgtagt cgcggtccga gtcgacgatg aggcatcggt ccatatcgaa
     1501 gactgccagg tctcggtcgc tgtaggagaa cgggttgctc atcgtcttgc ggacctgcga
     1561 cccgtggatg ggataatcgg ctggctcgcc ggcgaggagc ctggtgaacg cgagctcgtt
     1621 ttctttaatg aaatcctcgg gggcagagcc gacgtcggcg aagcaatagg ccaggtaggt
     1681 ctccgactcc ggctgctcgc gccggtttgc gacggcgggg ccgacctgct cgagc
//
LOCUS       DAOOLN010000403         1735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_88415_length_1735_cov_2.523214, whole genome shotgun sequence.
ACCESSION   DAOOLN010000403 DAOOLN010000000
VERSION     DAOOLN010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88415_length_1735_cov_2.523214"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="PLW80_05845"
     CDS             complement(<1..577)
                     /locus_tag="PLW80_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPB66063.1"
                     /translation="MAPELDDVHEIIQIDSELNKIRDYDILLERVLLEARRVVHADAG
                     SIYVKEGDVISIKFAHNDTKQKSLPPGEKQAYPHFSIPINDKTISGYVAMTGEAVNIP
                     DVYNLPPGLPFSYNTAYDAKVNYKTTSMLTVPLKSQNNVVLGVIQIINAMGPDGRVEA
                     FTEQDESLVTHFAANATVALQRAYMTRAMIIR"
     gene            708..1238
                     /locus_tag="PLW80_05850"
     CDS             708..1238
                     /locus_tag="PLW80_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HPB66064.1"
                     /translation="MLHAIGDAIPRVARSAFVAWNAEVAGDATIGEDSSVWFGVTVRA
                     DMAPIRIGSGTNIQDGSVVHVADETPCVVGDRVTVGHGVILHGCEVGDDCLVGMGATL
                     LNGSRIGPGSIVAAGALVTQGKAFPPRSMIMGSPAKAVRTISDEELERIRRNGLEYVA
                     MARETASYRRIDGDDD"
     gene            1286..>1735
                     /locus_tag="PLW80_05855"
     CDS             1286..>1735
                     /locus_tag="PLW80_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66065.1"
                     /translation="MESETSQPPEDQGLERNGLFGKKVFFLNPPGVIGDVANALAEAE
                     FEVYSTRDHAKLARFLRKEPECLTFVNIDEGDDESVWRAWIKSIKGEEASARAAFGII
                     TMLDDEDKRSYYLMDLGVECGFVVVKIGVAKTTEILLCMLEANEARGR"
BASE COUNT          297 a          525 c          600 g          313 t
ORIGIN      
        1 tgcgtatgat catcgcgcgg gtcatgtagg cgcgctgcag ggcgaccgtc gcgttggcgg
       61 cgaagtgggt gacgagggac tcgtcctgct cggtgaacgc ctcgacccgg ccgtccggcc
      121 ccatcgcgtt gatgatctgg atgacgccga gcaccacgtt gttctggctc ttgagcggca
      181 cggtcagcat cgaggtggtc ttatagttga ccttggcgtc gtaggcggta ttgtagctga
      241 acgggagccc cgggggcagg ttgtagacgt cggggatgtt gacggcctcg ccggtcatgg
      301 ccacgtagcc cgatatggtc ttatcgttta tggggatgct gaagtgggga taggcctgct
      361 tctcgcccgg cgggaggctc ttctgcttgg tgtcgttatg ggcgaacttg atcgagatga
      421 cgtcgccttc cttgacgtag atggagccgg cgtcggcgtg gacgacgcga cgcgcctcga
      481 gcaagacgcg ctcgagcagg atgtcgtaat cacggatctt attcaattcg gaatcgatct
      541 ggattatttc gtggacatcg tcaagctcgg gggccatcac ttctccttgt ctctctagta
      601 taccttatct actatcgtcc gtcgatgcaa aggaaaaaga tcggcgccgg cggtattttg
      661 acaagccggg gcccggcggc gatactgtcg gcctgatcgg agggactatg ctgcatgcca
      721 taggcgacgc cataccgcgc gtcgcgagga gcgccttcgt ggcgtggaac gccgaggtgg
      781 ccggcgacgc gaccataggc gaggactcgt ccgtttggtt cggcgtgacg gtcagggccg
      841 acatggcgcc gatccgcatc ggctcgggca cgaacatcca ggacggctcg gtcgtgcacg
      901 tggcggacga gacgccctgc gtcgtcggcg accgcgtgac ggtcgggcac ggagtcatcc
      961 tgcacggctg cgaggtcggc gacgactgcc tcgtcggcat gggcgcgacc ctcctcaacg
     1021 gctcgcgcat agggcccggc tccatcgtcg ccgcgggcgc cctcgtcacc cagggcaagg
     1081 ccttcccgcc ccgctcgatg atcatgggca gcccggccaa ggccgtacgg acgatcagcg
     1141 acgaggagct cgagcgcata cgccggaacg gcctcgagta cgtggcgatg gccagggaga
     1201 ccgcctcgta tcgccggatc gacggggacg acgactaggg cgcccaccct tgcgctcgcc
     1261 gcgccgcggc gatactcttc tacgcatgga aagcgaaacg agccagccgc cggaggatca
     1321 ggggctggaa cggaatggac tgttcggcaa gaaggtcttc ttcctgaacc cgccgggcgt
     1381 gatcggcgac gtggccaacg ccctggccga ggccgagttc gaggtctact cgacccgcga
     1441 ccacgccaag ctggcgcgct tcctgcgcaa ggagccggag tgcctgacct tcgtcaacat
     1501 cgacgagggc gacgacgaga gcgtctggcg cgcgtggatc aagtccatca agggcgagga
     1561 ggcttcggcc cgggccgcct tcggcatcat cacgatgctc gacgacgagg acaagcgctc
     1621 gtactacctg atggatctcg gcgtcgagtg cggcttcgtc gtcgtgaaaa tcggcgtggc
     1681 gaagaccacc gagatactcc tgtgcatgct cgaggccaac gaggcgcgcg gccgc
//
LOCUS       DAOOLN010000404         1734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_88490_length_1734_cov_2.879690, whole genome shotgun sequence.
ACCESSION   DAOOLN010000404 DAOOLN010000000
VERSION     DAOOLN010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88490_length_1734_cov_2.879690"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1442
                     /locus_tag="PLW80_05860"
     CDS             <1..1442
                     /locus_tag="PLW80_05860"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPB66066.1"
                     /translation="DSPYHHATRLRSDPGDIAVLSELGLDPSARNNEGDTALHLAARL
                     RAEAQGLALLAAGSDPLLANAAGATPLTTALTASGGPLVWFFTPEVLAAEDPSGNGPL
                     HHAAMAGLADGAAYLARLGVPVDGKNDDGQTALMLALKRDSTGTVNALLALGADPGIR
                     DSSGSTALHLAVYWRATECLKLLARSASSLDPRDYTGKTPLRDAVDRSDAQATAFLLE
                     RGADPLARDNSGEAPLHAAARIADDRFTSALAAKAGRVDVRDDSGATPLLEAVYAENA
                     RSARALASLGASIHSRDSAGESPLSYALKKGGAVLQALLDQKTARSSDADGRSVIRVI
                     LEAKPSIDFIELALAAGASVDDRDAFGRSPLHVAATNGYAEIAELLVAAGADRFVRDS
                     GGATPAVIALSRGDAMIAALFGASPNDADYLGETALHYAAAGGLDRAAQALLALGADT
                     ARRNAAGETPADVAARRGHAALAKLLAGE"
     gene            complement(1512..>1734)
                     /locus_tag="PLW80_05865"
     CDS             complement(1512..>1734)
                     /locus_tag="PLW80_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPB66067.1"
                     /translation="TLQDTISDVLKNLNQREITIITLRFGLNGEGPLTLEETGKLLGI
                     TRERVRQIQEKAVQKLKELRRLDEYQEIC"
BASE COUNT          262 a          628 c          578 g          266 t
ORIGIN      
        1 gcgactcgcc ctaccatcac gcgacgaggc tacgctccga cccgggcgat atagccgtcc
       61 tgtccgagct cgggctcgac ccgtcggctc gcaacaacga gggcgacacg gccttgcacc
      121 tggccgcccg cctgcgcgcc gaggcccagg gtctcgcgtt actagcggca ggatcggacc
      181 cgttgctcgc caacgcggct ggagccacgc cgctcacgac ggcgctcacg gcgtcgggcg
      241 ggccgctcgt atggttcttc acgccggagg tattggccgc cgaggacccg tcgggtaacg
      301 gacccctcca tcacgcggcg atggcaggcc tggccgacgg cgcggcctac ctggcgaggc
      361 tgggcgtacc ggtggacggc aagaacgacg acggccagac ggccctgatg ctggcgctca
      421 aacgcgactc gacaggcacc gtcaacgcgc tcctcgcgct cggggccgac cccggtatac
      481 gcgactcgtc cggctcgacg gccctccatc tggcggtcta ttggcgggct accgaatgcc
      541 tcaagctgct ggcccgttcg gcctccagcc tcgacccgag ggactatacc ggcaagacgc
      601 cgctacgtga cgccgtcgac cgctccgacg cccaggccac ggccttcttg ctcgagcgcg
      661 gcgcagaccc gctggcgcgc gataactccg gcgaggcgcc gctccatgca gcggccagga
      721 tagccgacga tcgcttcacg agcgctctgg ccgccaaggc cgggcgtgtc gacgtcaggg
      781 acgattcggg cgctaccccc ctgctcgagg ccgtctatgc cgagaacgcc aggtcggctc
      841 gggcgctcgc ctcgctcggc gcgtccatcc actcgaggga ctcggccggg gaaagccccc
      901 tcagctatgc cctcaagaaa ggcggcgcgg tactccaagc cctgctcgac cagaagacgg
      961 ccaggagctc ggacgccgac ggacgctcgg tgatcagggt gatactcgag gctaagccgt
     1021 cgatcgattt catcgagctg gccctggcgg ccggcgcctc ggtcgacgac cgcgacgcct
     1081 tcggacgttc gcctctccac gtcgcggcga cgaacggcta cgccgaaatc gccgagctac
     1141 tcgtcgcggc cggcgccgac cgattcgtgc gggactcagg cggcgccacc ccggccgtca
     1201 tagcgctgtc gaggggcgac gcgatgatag ccgcgctgtt cggcgcctcg cctaacgacg
     1261 ccgactacct cggcgagacg gccctccact acgcggccgc tggcggcctc gatcgggcag
     1321 cgcaggccct cttggccctc ggagcggata cggcgcggcg caacgcggcc ggagagactc
     1381 cggccgatgt cgcggctcgc cgcgggcacg cggctctggc gaagctccta gcgggcgagt
     1441 aagccgagac taaaacaggg ccgtccggat cgcttccgga cggccctgtt ttcgtaggcg
     1501 ctcatcgcca atcagcagat ttcctggtat tcgtccagac ggcgcagttc cttgagcttc
     1561 tggacggcct tctcctggat ctggcggacg cgctcgcgcg tgatgccgag cagcttgccg
     1621 gtctcctcga gcgtcagcgg cccctcgccg ttcaggccga agcgcagcgt gatgatagtt
     1681 atctcgcgct ggttgaggtt cttgagcacg tcgctgatgg tatcctgcag cgtc
//
LOCUS       DAOOLN010000405         1734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_88501_length_1734_cov_2.661703, whole genome shotgun sequence.
ACCESSION   DAOOLN010000405 DAOOLN010000000
VERSION     DAOOLN010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88501_length_1734_cov_2.661703"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLW80_05870"
     CDS             <1..343
                     /locus_tag="PLW80_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66068.1"
                     /translation="YELQLYKSDPPQDALAFTGSPRKHPYDDGKIILLVEPNAGESAI
                     YEFKAADIVGAQDLPSPVTEAGESYRIVRLWIRRGAVGIRYEPFEVEAPLRFMNDAKA
                     FKDKMLGRHRG"
     gene            297..1376
                     /gene="amrS"
                     /locus_tag="PLW80_05875"
     CDS             297..1376
                     /gene="amrS"
                     /locus_tag="PLW80_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPB66069.1"
                     /translation="MRRRSRTKCSAGIEARAVDPRAGPVARYYECLPDGDIRCRLCPR
                     RCVIRDGGAGACRVRSNSGGTMTLPFYGMASAVSADPIEKKPLYHFMPGSATYSVGYL
                     GCNLHCPFCQNHGISQRTDAPTWRVEPAGLIAEARAARCPSLAHTYSEPLAHAEFVMD
                     CMKAAREAGLANVLVTNGCCGEEAAADVLSLCDAVNVDVKAWDEGFYREELGGDLNSV
                     KRFVMMAVEYDVHVEATTLVIPGKNDDPAQIDAIAGFLAALSPDIPYHLSAYHPMYRY
                     TIPATKASTLRSLADLARRRLRYVYSGNLPGERSLTLCRACGAVLVSRMGYAVDASGL
                     RGCRCASCGEPSPIHGARKKNQKCP"
     gene            1421..1492
                     /locus_tag="PLW80_05880"
     tRNA            1421..1492
                     /locus_tag="PLW80_05880"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1453..1455,aa:His,seq:gtg)
     gene            1535..1608
                     /locus_tag="PLW80_05885"
     tRNA            1535..1608
                     /locus_tag="PLW80_05885"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1569..1571,aa:Arg,seq:tcg)
     gene            1635..1707
                     /locus_tag="PLW80_05890"
     tRNA            1635..1707
                     /locus_tag="PLW80_05890"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1668..1670,aa:Lys,seq:ctt)
BASE COUNT          312 a          576 c          530 g          316 t
ORIGIN      
        1 ctacgagcta caactctata agagcgaccc tcctcaggat gcgctcgcct tcaccggctc
       61 gccgcgcaag cacccctacg atgacggcaa gatcatcctg ctcgtcgaac cgaatgccgg
      121 cgagtcggcc atctacgaat tcaaggcggc cgacatcgtc ggagcccaag acctgccgag
      181 ccccgtgacc gaggccgggg agagctaccg catcgtccgc ctctggatac gccgcggggc
      241 ggtcggaata cgctatgagc cgttcgaggt cgaggccccg ctacggttca tgaacgatgc
      301 gaaggcgttc aaggacaaaa tgctcggccg gcatagaggc tagagccgtg gatcctcgcg
      361 ccgggccggt ggcacggtac tatgaatgtc tccccgacgg ggacatccgt tgccggctat
      421 gcccgcgccg ctgcgtcata cgcgacggcg gcgcgggagc ttgccgggtc aggagcaact
      481 caggcggaac catgacgctt ccgttctatg gcatggccag cgccgtatcg gccgacccga
      541 tcgagaagaa gccgctgtac cacttcatgc cgggtagcgc aacctactcc gtcggctacc
      601 taggctgcaa cctccactgc cccttctgcc agaaccacgg catctcgcaa cgcaccgatg
      661 ccccgacctg gcgcgtcgag ccggccggcc tcatcgccga ggcgagggcg gcgcgttgcc
      721 catcgctggc tcatacctat tcggagccgc tcgcgcatgc ggaattcgtc atggactgca
      781 tgaaagccgc cagggaggcc ggccttgcca acgtactcgt caccaacggc tgctgcggcg
      841 aggaggccgc ggccgacgtc ctgtcgctgt gcgatgccgt caacgtcgat gtgaaggcct
      901 gggacgaggg cttctatcgc gaagagctgg gcggcgacct gaactcggtc aagcgcttcg
      961 tgatgatggc tgtcgagtac gacgtccacg tcgaggcgac cacgctggtc attcccggca
     1021 agaacgacga cccggcccag atcgacgcca tagccggctt cctagccgcc ttgtcgcccg
     1081 acataccgta ccacctgtcg gcgtatcatc ccatgtaccg ctatacgatt ccggcgacga
     1141 aggcctcgac gctacgctcc ctcgctgacc tggcccgccg tcgcctgcgc tacgtctact
     1201 cgggcaacct accgggcgag cgatccctca cgctatgccg ggcttgcggc gccgtcctcg
     1261 tctcgagaat gggctacgcc gtcgacgcct ccggactacg gggctgccgc tgcgcctcct
     1321 gcggcgagcc gagcccgatt cacggcgcta gaaaaaaaaa ccagaaatgc ccttgacact
     1381 ttttttgcgt tttggcatag tacgtctcgc caagcgcacg gtggatgtag ctcagcggta
     1441 gagtcccaga atgtggatct ggttgtcgcg ggttcgaacc ccgtcatcca ccctagctcg
     1501 tccgtaaccg gcgctcgggg ccgatcggtg gcgggcgttc gtagctcaga tggatagagc
     1561 gacggacttc gaatccgtag gtcgccggtt cgagtccggc cggacgcagt ctaaggtttt
     1621 tttgtagagt accagggccg ttagctcagc tggtagagca gcagactctt aatctgcggg
     1681 tcgcaggttc gatccctgca cggctcattc gatgctccca tgcgattgtt cgct
//
LOCUS       DAOOLN010000406         1731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_88734_length_1731_cov_2.820406, whole genome shotgun sequence.
ACCESSION   DAOOLN010000406 DAOOLN010000000
VERSION     DAOOLN010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88734_length_1731_cov_2.820406"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /gene="rocD"
                     /locus_tag="PLW80_05895"
     CDS             <1..871
                     /gene="rocD"
                     /locus_tag="PLW80_05895"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HPB66070.1"
                     /translation="AIKAARRWGAEVKGVPNGKQEVICADDNFHGRTLVAISMSNDPD
                     STVNYGPYVPGFVRVPYGDADAIVKAITPNTVAVIVEPIQGEAGVVVPPAGYLAKIRE
                     ACDKNKVLFIADEVQTGFCRTGKRFAWQHEGARPDIMCLGKALGGGVMPVSAIVADQR
                     IMKVFTPGTHGSTFGGNPLSSSVAIAALEVLEDEKLDQNAERLGKIFRDEVAKIKCPR
                     MVTVRGKGLLNAVVFEKGFEAWNVCLSLRDAGLLAKQTHGNIIRFAPPLVITEKEMME
                     AVAIIKRVFEKIA"
     gene            1009..>1731
                     /locus_tag="PLW80_05900"
     CDS             1009..>1731
                     /locus_tag="PLW80_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HPB66071.1"
                     /translation="MKSPKETSLERKLIEAARGEALRAAELLIADEEAQALQEYANVV
                     SIRRLNFNDHGPVHMRTVAINALRMLDLLQAAAVKTSLETNGAGSIEDSRVAVLMAAF
                     LHDVGMSIGRQNHEMNSAILAAPIIDRILQVMYPGETMRRSAIKCLALEGIEGHMATM
                     RIHSVEAGLILVADGCDMEKGRSRIPMMLSVEPRVGDIHQYSAAAISKVTIGPGEHRP
                     IRIDVSMEESVGFFQVEEVLLQK"
BASE COUNT          335 a          586 c          530 g          280 t
ORIGIN      
        1 cgcgattaag gccgcccgcc gctggggcgc cgaggtcaag ggcgtgccga acggcaagca
       61 ggaagtgatc tgcgccgacg acaacttcca cggccgtacc ctcgtcgcca tctccatgtc
      121 gaacgacccc gattctaccg tcaactacgg cccctacgta cccgggttcg tgagggtgcc
      181 ctacggcgat gccgacgcta tcgtcaaggc catcacgccg aacaccgtgg ccgtcatcgt
      241 cgagcccatc cagggagagg ccggcgtcgt cgtgccgccc gccggctacc tggccaagat
      301 ccgcgaggcc tgtgacaaga ataaggtcct gttcatagcc gacgaggtcc agacaggctt
      361 ctgccggacc ggcaagcgct tcgcctggca gcacgagggc gctcgtcccg acattatgtg
      421 cctcggcaag gccctcggcg gcggcgtcat gcctgtatcc gccatcgtcg ccgaccagag
      481 gatcatgaag gtcttcactc ccggcaccca cggctccacc ttcggcggca acccgctgtc
      541 gagctcggtc gccattgcgg ccctcgaggt gctcgaggac gagaagcttg accagaacgc
      601 cgagcgcctc ggtaagatct tcagggacga agtcgccaag atcaagtgcc ccaggatggt
      661 caccgtccgc ggcaagggcc tcctcaacgc cgtcgtgttc gagaagggct tcgaggcctg
      721 gaacgtctgc ctctctctac gcgacgccgg cctcctggcc aagcagaccc acggtaacat
      781 catccgcttc gccccgcccc tcgtcatcac cgagaaggag atgatggagg ccgtcgcgat
      841 catcaagcgc gtcttcgaga agatcgcctg atagcggcga cgagtcgccc aagaacgcta
      901 gccgcgcgct agcgcggcta cgccccggct taggccgggg cgcttttttt cggtatatcg
      961 tcgcgaagcg ccatcgcttc gcgagccgaa tgctgtatat tcatacgtat gaaaagcccc
     1021 aaggaaacga gcctcgagcg caagctcatc gaggccgcgc gaggcgaggc gctacgcgcc
     1081 gccgagctgc tcatcgccga cgaggaagcg caagccctcc aggagtacgc caacgtggtg
     1141 tcgatacggc gcctcaactt caacgaccac ggtcccgtgc atatgcgcac ggtcgcgatc
     1201 aatgcgctcc gcatgctcga cctgctccag gcggccgctg tcaagaccag cctcgagacc
     1261 aacggcgccg gctccatcga ggacagccgc gtggcggtac tgatggccgc tttcctgcac
     1321 gacgtgggga tgtcgatagg ccgccagaac cacgagatga atagcgccat cctcgcagca
     1381 cccatcatcg atcggatcct ccaggtgatg taccccgggg agacgatgcg gcgctcggcc
     1441 atcaagtgcc tggcgctcga gggcatcgag ggccacatgg ctacgatgcg catccactcc
     1501 gtcgaagccg gcctgatact cgtcgccgac ggctgcgaca tggagaaagg acggtcgcgc
     1561 atcccgatga tgctgtccgt ggaacccagg gtcggcgata ttcaccaata ctccgccgcg
     1621 gcgatctcca aggtgacgat aggtcccggc gagcatcggc cgatacgcat cgacgtgagc
     1681 atggaggagt cggtcggctt cttccaggtc gaagaggtcc tgctccagaa g
//
LOCUS       DAOOLN010000407         1730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_88797_length_1730_cov_3.299701, whole genome shotgun sequence.
ACCESSION   DAOOLN010000407 DAOOLN010000000
VERSION     DAOOLN010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88797_length_1730_cov_3.299701"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PLW80_05905"
     CDS             <1..452
                     /locus_tag="PLW80_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPB66072.1"
                     /translation="GDYGETEFMRDFGRALDLAYGLSPFRPTLPLNLSVQRSRRGPAF
                     SADDCEALDALNAQMNNLLAVAERLGRAEYAPWTAERVRDALPDLSRREAEVLALCAE
                     PLSMAQVASALAIGRRTAETHLAHVFEKLGVRSRREALDALWLRVPR"
     gene            463..1371
                     /locus_tag="PLW80_05910"
     CDS             463..1371
                     /locus_tag="PLW80_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPB66073.1"
                     /translation="MRVAATQLLLAQGDVEGNCERAIRLVRLAARERAEVVVLPEFFT
                     SGVAIDDGMLAVPRLNREARVLERLRAEARGLGIIISGSMLCIRDADVYNSMVVVHPD
                     GSTFVHDKDLPTQFENAYYAPGDERRTDGAFALALCWELLRRKTVAEIGPSASLVLAG
                     SCWWSLPVGTSNQELDEYNDELNASTPAAFAAAAGLPVVHASLVGRIRTRRSLARDEV
                     VERRLVGTTQIVDRYGRVVASIRDKGSDDLIVEDVELAPRGSRRDPGAGFWLATMRDE
                     YLRAWETEGRLGRELYDVNRARMLGT"
     gene            complement(1406..1540)
                     /locus_tag="PLW80_05915"
     CDS             complement(1406..1540)
                     /locus_tag="PLW80_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66074.1"
                     /translation="MILGLPWWSWALILVGLALFVPLKTYFVKKFLARKPEPPEDEES
                     "
     gene            complement(1551..>1730)
                     /locus_tag="PLW80_05920"
     CDS             complement(1551..>1730)
                     /locus_tag="PLW80_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66075.1"
                     /translation="ARAWFLAFPNVWMLELYRATFAGAKHSGYLVPLAATAVLGGALL
                     IPLSTIARKKLGLKL"
BASE COUNT          274 a          575 c          606 g          275 t
ORIGIN      
        1 ggggcgacta tggcgagacc gagttcatgc gcgatttcgg tcgggccttg gacctggcct
       61 acggcctgag cccgttcagg ccgaccctgc cgctcaacct gtcggtccag cgctcgcgcc
      121 gcggaccggc cttcagcgcc gacgactgcg aggccctgga cgcgctcaac gcgcagatga
      181 acaacctgct cgccgtggcg gagcggctcg ggcgggccga gtacgcgcca tggaccgcgg
      241 agcgcgtacg cgacgccctg cccgacctga gccgccgcga ggccgaggtg ctcgccctgt
      301 gcgccgagcc cctgtcgatg gcccaggtag cctccgccct ggcgatcggc cgccgtacgg
      361 ccgagaccca tctggcccac gtcttcgaga agctaggcgt ccgctcgcgc cgcgaggcgc
      421 ttgacgcgct ctggctgagg gtcccgaggt gagggcgtac gcatgagggt ggcggcgacg
      481 caactgctgt tggcccaggg cgacgtcgag ggcaactgcg agcgggcgat acgcctcgtg
      541 aggctggctg cccgcgagcg tgctgaagtc gtcgtcctgc ccgagttctt cacctcgggc
      601 gtagccatcg acgacggcat gctcgccgtg ccgaggctga accgcgaggc gcgggtgctc
      661 gagcgcctgc gggccgaggc gcggggcctc ggtatcatca tatccgggtc gatgctgtgt
      721 atccgggacg cggacgtcta caattcgatg gtcgtcgtcc atcccgacgg atcgaccttc
      781 gtccacgaca aggatctgcc gacccagttc gagaacgcct actacgcgcc gggcgatgag
      841 cggcggaccg acggcgcctt cgcgctcgcg ctgtgctggg agttgctgcg gcgtaagacc
      901 gtcgccgaga tcgggccgag tgcgagcctt gtgctcgccg ggtcctgctg gtggagcctg
      961 cctgtcggca cctcgaacca ggagctcgac gagtataacg acgagctgaa cgcgtcgacg
     1021 cccgccgcct tcgctgccgc ggctggcttg cccgtcgtcc acgcgtcgct cgtcggccgg
     1081 atcaggacca ggcgctccct cgcgcgcgac gaggtcgtgg aacgtcgcct ggtcggcacg
     1141 acccagatag tcgaccggta cggtcgagtc gtcgcgtcga tacgggacaa gggctcggat
     1201 gatctgatcg tggaggatgt cgagttggca ccccgcggtt cacggaggga tcccggcgcc
     1261 ggcttctggc tggctacgat gcgcgacgag tacctgaggg cctgggagac cgagggtcgg
     1321 ctcggccggg agctgtacga cgtgaatcgc gcgcgcatgc tcgggacgta gccggatccc
     1381 cgatccgccg cgcgacctgg aagtatcagc tctcctcgtc ctccggcggt tccggcttcc
     1441 tggccaggaa cttcttgacg aagtacgtct tgagcggcac gaaaagggcc agcccgacga
     1501 ggatgagcgc ccaggaccac cacggtaaac caagaatcat atcgacctcc ttatagcttc
     1561 aagccgagct tcttacgggc gatcgttgaa agcgggatca acagggcgcc acccaggacg
     1621 gcggtcgccg cgagcggaac caggtatcca gaatgtttgg cgccggcgaa cgtcgcccgg
     1681 tacagttcga gcatccagac gtttgggaac gcgaggaacc acgcgcgcgc
//
LOCUS       DAOOLN010000408         1730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_88804_length_1730_cov_2.926567, whole genome shotgun sequence.
ACCESSION   DAOOLN010000408 DAOOLN010000000
VERSION     DAOOLN010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88804_length_1730_cov_2.926567"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            417..1244
                     /locus_tag="PLW80_05925"
     CDS             417..1244
                     /locus_tag="PLW80_05925"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HPB66076.1"
                     /translation="MALDGKLVITCACTGAETTKAQNPALPITPDEVARTAEECRAAG
                     AAILHLHVRNDDGTPTASVARFKEAIEAVRRKTDIIVEITTGGAVGDTAEERLRPVKE
                     LEPEMASLDCGTVNFGDEYIVNDLPTMRAFAAEFRRRKVRATLECFDLGHVYASHYLI
                     KEGLIEAPYHYGFVLNVPGAVPYSVENLSAFIRALPAGADYTVMGIGRSSLPAQFGAM
                     ANGGWIRVGFEDNVYFSKGVLAKSNAELVERAARIAREGGLKPATPDEVRQYLKLRG"
     gene            1392..>1730
                     /locus_tag="PLW80_05930"
     CDS             1392..>1730
                     /locus_tag="PLW80_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66077.1"
                     /translation="MHTYPSYDYKRIPLYKDVTPEQWNDWRWQMKNDIKDVETLAKVV
                     PLSDKEKADISEVLKVFRMAITPYYASLIDPANPGCPVRLQAIPRLPETHFDESDQDD
                     NGAVLDGPRFL"
BASE COUNT          318 a          566 c          552 g          294 t
ORIGIN      
        1 aacgtgagaa gacgggagac cgtctctcgc cccgggggct ccccgtcccc ggcgcggcga
       61 gcgcgcgcgg ccttgcggca tgacgcatgg acgagcccgc gtgagccgga acgtatcgga
      121 atcgggtctg gaagggcccg gaggatggac gagaccatcc gtgccgattc taccgtgtac
      181 ccgttctctg attcttgatc tatccatggg gtcgtgtcgc cggtcggcgg tcctgaaagc
      241 gacgccggat cgaacggaat gtccggaagt gccttcggcg ttactgcctt cggcgttact
      301 gccttcggcg ttaccgcgca ttccgttcga tctggccgag tcgggtctcc ggtccggtcc
      361 gacgccaccc cgctatcgta gcgccgctgg aacccgcggc gatggaagga gtacatatgg
      421 ctcttgatgg taagctcgtc atcacctgcg cctgcaccgg cgcggagacc acgaaggccc
      481 agaatcccgc gttgcccatt acccccgacg aggtggccag gaccgccgag gagtgccgcg
      541 cggccggcgc cgccatcctg cacctgcacg tgcgcaacga cgacggcacc cccacggcga
      601 gcgtcgcccg cttcaaggaa gccatcgagg ccgtccgcag gaagaccgac atcatcgtcg
      661 agatcaccac cggcggagcc gtcggcgaca cggccgagga gcgcctgagg ccggtcaagg
      721 agctcgagcc ggaaatggcg tcgctcgact gcggcaccgt caacttcggc gacgagtata
      781 tcgtcaacga cctgccgacg atgcgcgcct tcgccgccga gttcaggcgt cgcaaggtgc
      841 gggcgacgct cgagtgcttc gaccttggtc acgtctatgc gagccactac ctgatcaagg
      901 aaggcctgat cgaggcgccg taccactacg gcttcgtgct caacgtgccc ggcgccgtgc
      961 cgtactcagt cgagaacctg tcggccttca tccgcgcgtt gccggccggg gccgactaca
     1021 ccgtgatggg catcggccgc tcatcgctgc ccgcccagtt cggcgcgatg gcgaacggcg
     1081 gctggatacg cgtcggcttc gaggataacg tgtacttctc gaagggcgtc ctcgccaaga
     1141 gcaacgccga gctggtcgag cgcgccgcgc gcatcgcgcg cgagggcggc ctcaagccgg
     1201 ccacccccga cgaggtccgc cagtacctga aactacgggg ctgataccga gaatatggcc
     1261 acggggtcac ggaggcgcgg agaagacaag agagtgatta aggaaatgag aaggttccag
     1321 gcttatcctt ttctttcctc cgcgtctccg tgtctccgtg gttgattttt ctcttttaag
     1381 gaggctttct aatgcatacg tatccgtcat acgactataa gcgcataccc ctgtacaagg
     1441 acgtgacgcc cgagcagtgg aacgactggc gctggcagat gaagaacgac atcaaggatg
     1501 tggagacgct cgccaaggtc gtcccgctga gcgacaagga gaaggccgac atctccgagg
     1561 tgctcaaggt cttccgcatg gcgatcacgc catactacgc gtcgctcatc gacccggcca
     1621 atcccggctg cccggtcagg ctccaggcca tcccgcgcct gccggagacg cacttcgacg
     1681 agagcgacca ggacgacaat ggtgccgtac tggatgggcc gcgtttcctc
//
LOCUS       DAOOLN010000409         1730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_88805_length_1730_cov_2.917612, whole genome shotgun sequence.
ACCESSION   DAOOLN010000409 DAOOLN010000000
VERSION     DAOOLN010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88805_length_1730_cov_2.917612"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1067)
                     /locus_tag="PLW80_05935"
     CDS             complement(102..1067)
                     /locus_tag="PLW80_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPB66078.1"
                     /translation="MVPTAAVGIALDDTRLLADVCGVNDYNLRVLERLLGVPVFVRGN
                     EISLESPRPDVRELFAAVIEQLLSSIDDGLPASPELITAIHASLTPDGQAAPSGASFK
                     DSAIQVPGAFSKVYPRTANQALYLRGLSSHDISVAVGPAGTGKTYLAVAWALREVLSK
                     ARRRLVLTRPVVEAGESLGFLPGDLGQKISPYLRPLYDAMEALVPYDVVKRLEDNHTI
                     EIAPLAYMRGRSLNNCVVILDEAQNTTKEQMKMFLTRLGEGSVAVVTGDATQIDLPRK
                     SESGLVHALKVLSDIDDIFVCRLDGRDVVRNPLVRKIIDAYESEI"
     gene            complement(1123..>1730)
                     /locus_tag="PLW80_05940"
     CDS             complement(1123..>1730)
                     /locus_tag="PLW80_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66079.1"
                     /translation="AVAYRLSYGAGWSSVGDPSFMVNSMSMAYKTSWKPPAAWRRRIQ
                     WTMDVNAAAQQSFLRFTDSYLNFVYGLTFKVHELLDLSLSSTSRNASLWRYFPAAFEI
                     PEEIALDPVNPLTDLLASFNFFDGTGEARRASLFKLKALELTATHHLHDWDLALKLTA
                     SPVLENLEYVFKTDFSVTLSWNSVSQIKSSYSKKGDTVSWD"
BASE COUNT          309 a          557 c          575 g          289 t
ORIGIN      
        1 cggcgacgca cacgagggcc gcgacggcta tcgccccgta gcggcggacc atggcgcggt
       61 cgctgaacgc ggcgcgcagg cggtcgagcc ggccgtaact atcagatctc tgattcatag
      121 gcgtcgatta tcttcctgac caggggattc ctgaccacgt ccctgccgtc gagacggcac
      181 acgaagatgt cgtcgatgtc cgacagcacc ttgagcgcat ggacgagccc ggactccgat
      241 ttacgaggca ggtctatctg ggtcgcgtcg ccggtcacca cggccaccga cccctccccg
      301 agcctcgtca ggaacatctt catctgttcc ttggtggtat tctgcgcctc gtccaggatg
      361 acgacgcagt tattgaggct ccggccgcgc atgtacgcga gcggggctat ctctatggta
      421 tggttatcct cgagccgctt gaccacgtcg tagggcacca gggcctccat cgcgtcgtac
      481 aggggccgca ggtaggggct gatcttctgc cccaggtcgc cgggcaggaa gccgaggctc
      541 tcgcccgcct ccaccaccgg ccgcgtcagc accagccgcc tgcgcgcctt ggacagcacc
      601 tcgcgcaggg cccaggccac cgccaggtag gtcttgcccg tgccggccgg accgaccgcc
      661 acgcttatgt cgtggcttga gagcccgcgc aggtacaggg cctggttggc cgtgcgtggg
      721 tagaccttgg agaaggcgcc gggtacctgg atcgccgagt ccttgaagga cgcgccggag
      781 ggggccgcct ggccatcggg cgtcaggctg gcgtggatcg cggtgatgag ctcgggagag
      841 gccgggaggc cgtcgtcgat cgacgacagg agctgttcga tcacggccgc gaacagctcc
      901 cggacgtccg gacgcggcga ctcgagcgag atctcgttgc cgcgaacgaa cacgggcacc
      961 cccaagaggc gctcgagcac gcgaaggttg taatcgttga cgccgcagac gtcggctagg
     1021 agcctcgtgt cgtccagggc gatgcctacg gccgctgtgg gaaccacggt gtccgatcct
     1081 aaccccgccg ggggaaaaaa tcaaggaagc cgggcccgcc tgtcagtccc aggagaccgt
     1141 atcgcccttc ttggaatagg aggacttgat ctgggacacc gaattccatg acagcgtcac
     1201 cgagaagtcg gtcttgaaca cgtactcgag gttctcgagc accgggctgg ccgtcagctt
     1261 gagcgccagg tcccagtcgt gcaggtggtg ggtagcggtc agctcgagcg ccttgagctt
     1321 gaacagcgac gccctgcgcg cctcgcccgt accatcgaag aaattgaacg acgccaggag
     1381 gtcggtcagc ggattgaccg gatcgagggc tatctcctcg ggtatctcga acgcggcggg
     1441 aaaatagcgc cacagcgacg cattcctgct cgtcgacgac aggctcaggt ccaggagttc
     1501 gtgcaccttg aaggtcaggc cgtagacgaa gttcaggtac gagtcggtga agcgcaggaa
     1561 actctgctgc gcggccgcgt tgacgtccat cgtccactgg atacggcggc gccaggccgc
     1621 cggcggcttc cagctcgtct tgtaggccat cgacatcgag ttgaccatga acgacgggtc
     1681 gccgaccgac gaccagccgg cgccgtagct caaccggtac gcgaccgcct
//
LOCUS       DAOOLN010000410         1727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_89089_length_1727_cov_2.169856, whole genome shotgun sequence.
ACCESSION   DAOOLN010000410 DAOOLN010000000
VERSION     DAOOLN010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89089_length_1727_cov_2.169856"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /locus_tag="PLW80_05945"
     CDS             <1..1230
                     /locus_tag="PLW80_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66080.1"
                     /translation="GGPAAVARLSRTRPTGLVQLWLALSSLRSADDELRSRDRPGSAL
                     RAVSAYLVGFAIMRTATNAYLFLSTLGRLASPAEIRGSAAVLVALACAPLLSAACLSG
                     DRTGAERRLALLPLSRPLRLTIAALSPAASAATIALLASILPAIAAAPFALRSIGLWL
                     AVAAWSSFTATAAGLGLRSAASAITAATRRSRPRNRGHGRRVALLAAMLASAAANPSP
                     SMAAGRLVVTLFGSVELAAADLANLAGRLSVDSMAALAAVAGSFALAAAIAEDAVLSG
                     GRRAEPRRRPARSPSSGRGASLPGGRLAYPLAMAAAAARGRRLGPALAASLAFSSLCV
                     SQGAPTAIPLTAAAAFLAVTLGSALASMAADSQTTRRFAMLGDGAAERGYLAASAILA
                     MVTAAPLCAAALAAAFR"
     gene            1311..>1727
                     /locus_tag="PLW80_05950"
     CDS             1311..>1727
                     /locus_tag="PLW80_05950"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HPB66081.1"
                     /translation="MAACTIGMNGSRISVAILAGGESRRIGRDKGLLPIGEKCMIEAV
                     AERLSPYADELIIGSNDAAAYAFTGFRVVPDLERGHGPMMGVLSCLEAARNGSVLFAP
                     CDVPVIPDAFVRLMLETAMDADIAMPLDELGRPEPLL"
BASE COUNT          210 a          631 c          620 g          266 t
ORIGIN      
        1 ggaggacctg ctgccgtggc tcggctgagc cgcaccaggc cgacggggct cgtccagctg
       61 tggctagcgc tgtcatcgct gcgctccgcc gacgacgagc tacgctcgcg ggaccggcct
      121 ggctcggcgc tccgggccgt gagcgcctac ctggtaggct tcgcgatcat gaggaccgcg
      181 acgaacgcgt acctgttcct gtctacgctc gggcgcctgg cgagcccggc tgagatccgc
      241 ggttcggcgg ccgtactcgt cgcgctggcc tgcgcgcccc tcctgtcggc cgcctgcctg
      301 tcgggcgacc gtaccggagc cgagcgcagg ctggcgctcc tgccgctatc gaggccgctc
      361 aggctgacca tagccgcgct ttcacccgcg gcctcagccg cgacgatagc cctgctcgcc
      421 tcgatcctgc cggccatagc ggccgcgccg ttcgcattac gctcgatcgg cctctggttg
      481 gccgtcgcgg catggtcatc gttcacggcg acggcggccg ggctcggcct acgctcggcc
      541 gcctcggcga taaccgccgc gacgcgccga tcgcggccgc ggaaccgcgg ccacggacgc
      601 cgcgtagcgc tgctcgccgc gatgctggcc tcggcggcgg ccaacccgag cccgagcatg
      661 gcggcgggcc ggctcgtcgt gacgctgttc ggttccgttg agctggccgc cgccgacctg
      721 gcgaacctcg ccggccgcct gagcgtagac tcgatggcgg ccctcgccgc ggtggccggg
      781 tccttcgcgc tggcggcggc catcgccgag gacgccgtac tgagcggcgg gcgccgtgcc
      841 gaaccccgac ggagaccggc caggtcgcca tcgtcaggac gcggcgctag cctgccgggt
      901 ggccggctag cctatccgct ggcgatggca gccgcggccg cccgtggacg gcgcttgggc
      961 ccggccctgg ccgcgtcgct agccttctcg tcgctgtgcg tctcgcaggg agcgccgacg
     1021 gccattccgc ttacggcggc cgcggctttc ctcgccgtga cgctcggctc ggcgctcgcc
     1081 tccatggccg ccgatagcca gacgacgcgt cgcttcgcca tgctcggcga cggcgcggcg
     1141 gagcggggct acctggcggc ctcggccatc ctcgccatgg ttacggccgc cccgctctgc
     1201 gcggcggccc tggccgccgc gttccgctaa tggtatatcg tcgcggcggc cggaaggccg
     1261 ccgccaaacc aagccatgac gaagcgaccg agggccggta tagtaggccc atggcagcgt
     1321 gtaccatcgg gatgaacggg tctaggatca gcgtcgcgat cctggctggc ggggagagcc
     1381 ggcggatagg acgggataag ggcctcctcc cgatcggcga gaagtgcatg atcgaggcag
     1441 tcgcggagcg gctgtcgccc tacgccgacg agctgatcat cggctccaac gacgcggctg
     1501 cctacgcgtt caccggcttc cgcgtggtgc cggatctgga gcgcggccac gggccgatga
     1561 tgggcgtgct gtcctgcctt gaggcggcgc ggaacggctc ggtgctgttc gcgccgtgcg
     1621 acgtaccggt gataccggac gccttcgtac ggctcatgct cgagacggcc atggacgcgg
     1681 atatagcgat gccgctcgac gaactcggcc gaccagaacc cttgctc
//
LOCUS       DAOOLN010000411         1726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_89137_length_1726_cov_2.774985, whole genome shotgun sequence.
ACCESSION   DAOOLN010000411 DAOOLN010000000
VERSION     DAOOLN010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89137_length_1726_cov_2.774985"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="PLW80_05955"
     CDS             <1..819
                     /locus_tag="PLW80_05955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB66082.1"
                     /translation="LRCPDCGLVRMTHFFPRDNPYAERAYFFDEYKAQYGRTYIDDIP
                     SIRTACSKRLAVIESMLPAGHEGQGVLDVGCAYGAFVAEAQARGWNAVGSDLAPDAVE
                     YVKSTWKIPAFVGDFAAPAADGLYPRNLACLSMWYVIEHFDELGRVLRRVASLLRPGG
                     VFAFSTPSGEGISAKRDARRFYERSPDDHYTVWSPSTAPGILKRFGFDVQRIVVTGHH
                     PERFPGVPAEADSLRYGAAMTVSRLLGLGDTFECYAVYRGRVEERVARRSAERT"
     gene            816..>1726
                     /locus_tag="PLW80_05960"
     CDS             816..>1726
                     /locus_tag="PLW80_05960"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPB66083.1"
                     /translation="MRSILILGAGFMQGPAIRIAKAKGYRVYAADADPNAAHAGSADE
                     VLHIDLKDKEALAEAAAGISGLAGVFTAGTDFSASVAYVAERLGLPGIPYEAALDASD
                     KARMRSRLRAAGVPVPDYAFGGSEDDPVALAEAVGSWPVVVKPVDNMGGRGCRLARSV
                     SELRDAWADAVSHSRTGRAIVEAYLDGPEFSIDAIVRDGIVSLRGVADRVVVFEPYFV
                     EMGHTMPTAYGEEVTGEVVAVFEAGVAALGIRNGAAKGDMKYTRRGAFVGEIAARLSG
                     GYMSAGPIRTRADSSRRPRPSTSPAAS"
BASE COUNT          254 a          594 c          587 g          291 t
ORIGIN      
        1 ctgcgctgcc cggactgcgg gctggtcagg atgacgcatt tcttcccgcg ggataacccg
       61 tacgccgaac gggcctattt cttcgacgaa tacaaggccc agtacggccg cacctatatc
      121 gacgatatcc cgagcatccg tacggcctgt tccaagcgtc tggcggtgat cgagtccatg
      181 ctaccggccg gccacgaggg gcagggcgta ctcgacgtgg gctgcgccta tggcgccttc
      241 gtggctgagg cccaggcgag gggctggaac gcggtcggct cggacctggc gcccgacgcc
      301 gtcgagtacg tcaagagcac ctggaagatc cctgccttcg tcggcgactt cgccgccccg
      361 gcagccgacg gcctctatcc tcggaacctc gcctgcctgt ccatgtggta cgtcatcgag
      421 cacttcgacg agctgggccg ggtgctccgg cgggtagcct cgctcctgcg gccaggcggc
      481 gtgttcgcct tctcgacgcc gtccggcgag gggatctcgg ccaagcgcga cgcccgccga
      541 ttctacgagc gatcgccgga cgaccactac accgtctgga gcccgtccac ggcgcccggg
      601 attctcaagc gcttcggttt cgatgtacaa cgtatcgtcg tcaccggcca ccatcctgag
      661 cgcttccccg gcgtacccgc cgaggcggat tcgctgcgct acggggccgc tatgactgta
      721 tcgagactgt tggggctcgg cgacaccttc gagtgctacg ccgtctatcg cggcagggta
      781 gaggaacggg tcgcccggcg atccgcggag cgaacatgag atccatactg atactcgggg
      841 ccggcttcat gcaggggccg gccatacgga tagccaaggc caagggctac agggtctacg
      901 cggccgacgc cgacccgaac gcggcccacg cgggctcggc agacgaagtc ctgcatatcg
      961 acctcaagga caaggaagcc ctcgccgagg cggccgccgg catctccggc ctcgccggcg
     1021 tgttcaccgc cggcaccgac ttctcggcgt cggtcgccta cgtcgccgag cggctcggcc
     1081 tgcccggcat cccctacgag gcggccctcg acgcatccga taaggccagg atgcgttcga
     1141 ggctccgggc ggccggcgtg ccggtgcccg attacgcctt cggcggctcg gaggacgacc
     1201 cggtggcctt ggccgaggcg gtcggctcct ggccggtcgt cgtcaagccg gtcgataaca
     1261 tgggcggccg tggctgccgc ctggctcgat ccgtctcgga gttgcgcgac gcctgggccg
     1321 acgccgtctc gcattctcgc accgggcgcg ccatcgtcga ggcctacctc gacggccccg
     1381 agttcagtat cgatgccatc gtgcgtgacg gcatcgtctc tctgcgcggc gtcgccgatc
     1441 gcgtcgtcgt cttcgagccg tacttcgtcg agatggggca tacgatgccc acggcctacg
     1501 gcgaggaagt gacgggcgag gtggtcgccg tcttcgaggc gggcgtcgcg gctctcggta
     1561 tccggaacgg cgcggccaag ggcgacatga agtacacgag gcgcggcgct ttcgtcggcg
     1621 agattgccgc gaggctgtcc ggcggctata tgtcggctgg acctatccgt actcgagcgg
     1681 actcgagccg acggccgagg ccatcgacat cgcctgcggc gtcgtg
//
LOCUS       DAOOLN010000412         1725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_89197_length_1725_cov_2.983832, whole genome shotgun sequence.
ACCESSION   DAOOLN010000412 DAOOLN010000000
VERSION     DAOOLN010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89197_length_1725_cov_2.983832"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PLW80_05965"
     CDS             <1..582
                     /locus_tag="PLW80_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66084.1"
                     /translation="ASVFWSAECLYALGRLSDAEKLYRSLGERYPDSVKAEAAKYRLS
                     LIQFKYREDELLTLLKWSHEESLRIIEEFQRREKAYEQALAVYQKRYGEAQRGVAQTQ
                     ASLEDQVASLKLAMDDLAKRLQEKDARVVELEGELAGLSADDDRAAAEPSAAGGTVVA
                     SGEAELLAMKARALSLMQFYLERLAERSAGGSR"
     gene            579..1613
                     /locus_tag="PLW80_05970"
     CDS             579..1613
                     /locus_tag="PLW80_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66085.1"
                     /translation="MRRLTILALAAMAFTASAFGLEAKDGRIKLVVDDRTGRFSLYYL
                     VDVTRNRYEPLLYDQETRTTYATLMVDGKAFRLGDAPEFRIAVSREASGKVRVEYRSS
                     FCVVRQLFSFVTSPGAPMADGLTIQYSIENVSKAESTIGLRVLLDTYVGEKSAAHFVA
                     QGVGPLVSERALSGDYADSWVRGLGSAELSAANLQVLLGEPATRPDTLLAANWKRLND
                     SSWFFEANPSRNFTLLPYSINDSALALYYEPAPVRPGSTRAVVVGLSQAVEGYDAYVA
                     AKGDKPPVVAIAAPSATAPLDEMADLIAVRAVLEAIDAMLAAGQAPNAAELEALETTL
                     RRLEARKGQY"
BASE COUNT          293 a          579 c          567 g          286 t
ORIGIN      
        1 gcctcggtgt tctggtcggc ggagtgcctg tacgcgctcg gacgcctctc ggacgccgag
       61 aaactatacc ggtccttagg cgagcgatac cccgacagcg tcaaggccga ggcggccaag
      121 tatcggctca gcctgatcca gttcaagtac cgcgaggacg agctcctgac cctgctcaaa
      181 tggagccacg aggagtccct gcgcatcatc gaggaattcc agcgccgcga gaaggcctac
      241 gagcaggccc tcgccgtata ccagaagcgc tacggagagg cgcagcgagg cgtcgcccag
      301 acccaggcct cgctcgagga ccaggtcgcc agcctcaagc tggccatgga cgacctggcc
      361 aagcggctgc aggagaagga cgcccgcgtc gtcgagctcg agggtgagct ggcgggtttg
      421 agcgccgatg acgaccgagc ggccgccgag ccgtccgctg cgggcggaac ggtcgtcgcc
      481 tccggcgagg ccgagcttct ggcgatgaag gcccgggccc tgagcctgat gcagttctac
      541 ctggagcgcc tcgccgagcg ctccgccgga ggttctcgat gaggagattg acgatactcg
      601 ccctggcggc catggcgttc accgcctccg cgttcggtct cgaggccaaa gatgggcgga
      661 tcaagctcgt tgtagacgat cggaccggcc gcttctcgct ctattatctc gtcgatgtca
      721 cgcgcaaccg ctatgagccg ctcctgtacg accaggagac ccgtaccacc tacgccacgc
      781 tcatggtcga cggcaaggcc ttcaggctcg gcgacgcccc ggagttccgg attgccgtgt
      841 cgcgggaggc ctccggtaag gtacgcgtcg agtaccgctc cagtttctgc gtcgtcaggc
      901 aactgttctc gttcgtaacg tcgccaggcg ctcctatggc agacgggctg acgatccagt
      961 attccataga gaacgtctcc aaggccgagt cgacgatagg cctgcgggtc ctgctcgaca
     1021 cctacgtcgg cgagaagtcg gccgcgcatt tcgtggcgca aggggtcggt ccgctcgtct
     1081 cggagcgggc actgtccggc gactacgctg atagctgggt gcgcggcctc ggatcggccg
     1141 agctttcggc cgccaacctg caggtgttgc tcggagagcc ggctacccgg cccgataccc
     1201 tcttggccgc caactggaaa cggctgaacg actcgtcatg gttcttcgag gcgaacccgt
     1261 cgaggaactt tacgctactg ccgtactcga tcaacgacag cgccctggcc ctgtactacg
     1321 agccggcccc ggtacgcccg ggttcgactc gcgcggtggt cgtcgggctg tcccaggccg
     1381 tcgaaggcta cgacgcctac gtggcggcca agggagacaa gcctccagtc gtcgcgatag
     1441 cggctccttc cgcgaccgct cccctcgacg agatggccga cttgatagcc gtgcgcgccg
     1501 tgctcgaggc gatcgacgcc atgctcgcgg ctggccaggc cccgaacgcg gccgagctcg
     1561 aggccttgga gaccacgctc cggcggctcg aggcccggaa ggggcagtac tgatacccta
     1621 tggcgaagaa tccgctcgac gaggccgagg cgctgttcag acgccgtcgc tgggccaacc
     1681 tcatcagcct cctcgagccg ctgacagcgg tctatcgcga gtcgg
//
LOCUS       DAOOLN010000413         1725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_89231_length_1725_cov_2.124551, whole genome shotgun sequence.
ACCESSION   DAOOLN010000413 DAOOLN010000000
VERSION     DAOOLN010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89231_length_1725_cov_2.124551"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PLW80_05975"
     CDS             complement(<1..567)
                     /locus_tag="PLW80_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPB66086.1"
                     /translation="MKKTLIVASALLVALTLFGCAKKAEPVAEPSAVEPYKVAFIYIG
                     VPGDLGWTHEHDVARLELEAALGDKVKTAYMENVPEGPDATRIIRQYAQDGYDMIFAT
                     SFGYMDPMAEVAAEFPDVKFVHCSGYKTSDNMSVYFGRIYQARYLAGIVAGRMTKSNI
                     IGYPGAFPIPEVVRGINAFTLGARSVNPK"
     gene            737..1297
                     /locus_tag="PLW80_05980"
     CDS             737..1297
                     /locus_tag="PLW80_05980"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPB66087.1"
                     /translation="MRALYPWIELSNKKVADCRVFKVSSSERQSTSGKRGTFFSVDAP
                     DWAGVIPVVEAPEGRSFVMIRQFRHGTRHLSIEFPGGIVEPGEDPATAIARELIEETG
                     YRPEMIVPLGILSPNPAFMTNTFHAFVAEGCVLERAQCLDEHEEIEVLLVPEKEAIDM
                     IGAEDMGHALMTATLFFYERYRGKLD"
     gene            complement(1329..>1725)
                     /locus_tag="PLW80_05985"
     CDS             complement(1329..>1725)
                     /locus_tag="PLW80_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB66088.1"
                     /translation="SARGGSVAVFSSTSVKEPIDGLVLSNSLRSAVVALAKTLSVELA
                     PRGVRVNSLAPGRFATGRVERLDKATAERSGLSADEVRARSQAAIALRRYGEPDEFGR
                     TAAWLLSPASSYLTGQLVTVDGGSLRGSW"
BASE COUNT          299 a          565 c          573 g          288 t
ORIGIN      
        1 cttcgggttg accgagcgcg cgccgagcgt gaacgcgttg atgccgcgta cgacctcggg
       61 gatcgggaag gcgcccgggt agccgatgat gttcgacttg gtcatgcggc cggccacgat
      121 gccggcgagg tagcgggcct ggtatatcct gccgaagtag acggacatgt tatcggaggt
      181 cttgtagccc gagcaatgca cgaacttgac atccgggaac tcggccgcga cctcggccat
      241 cgggtccatg tagccgaacg acgtggcgaa gatcatgtcg tagccgtcct gggcgtactg
      301 gcggatgata cgggtggcgt ccgggccctc gggcacgttc tccatatagg cggtctttac
      361 cttgtcgccc agggcggcct cgagctcgag gcgggccacg tcgtgctcgt gcgtccagcc
      421 gaggtccccg gggacgccga tatagatgaa ggctaccttg tacggctcga cggcggacgg
      481 ctccgcgacc ggctcggcct tcttggcgca accgaacagg gtcagcgcta cgagcagcgc
      541 gctcgcgacg atcaacgtct ttttcatcag ttctctcctt atgaaacgcg atgcgtgata
      601 gccgtctaat cgatacaggc tcggtgcgcg gcgacggtgc aaccagtaaa caacgcgagc
      661 cggagcttgt caaatcgttt ctttgcatcg gcacggcggg cttgccggcg cgtgcctgaa
      721 ggactactat tccggtatgc gcgcgctata cccgtggata gagctctcga acaagaaggt
      781 agcggattgc agggtcttca aggtcagctc gagcgagcgg cagtcgacct ccggcaagcg
      841 cggtaccttc ttctccgtcg acgcgccgga ctgggccggc gtcattccgg tcgtcgaggc
      901 gcccgagggc cgttcgttcg tgatgatacg gcagttcagg cacgggacga ggcacctgtc
      961 catagagttc cccggcggca tcgtcgagcc tggagaggat ccggcgacgg cgatagccag
     1021 ggagctgatc gaggagaccg gctaccggcc cgagatgatc gttccgctcg gcatcctctc
     1081 cccgaacccg gccttcatga ccaatacctt ccacgccttc gtcgccgagg gatgcgtcct
     1141 cgagcgggcc cagtgcctcg acgagcacga ggagatcgag gtgctcctcg tgcccgagaa
     1201 agaggccatc gacatgatag gcgcggagga catgggccac gcgctgatga cggccaccct
     1261 gttcttctac gagcgctacc gcggcaagct cgactagccg gagccgcccg gagtcgcccg
     1321 gagccgccct accaagagcc gcgcaggctg cccccgtcga cggtgacgag ctggccggtc
     1381 aggtacgacg acgccggtga caggagccac gccgccgtac gaccgaattc gtccggctcg
     1441 ccataacgcc ggagcgcgat ggccgcctgg gacctggccc ggacctcgtc ggcgctcagt
     1501 cctgagcgct ccgccgtcgc cttgtccagc cgttccaccc taccggtcgc gaagcgcccg
     1561 ggcgccaggc tgttgacccg tacgcctctg ggcgccagct ccacggacag cgtcttggcc
     1621 aaggcgacga ccgccgaccg tagcgagttg gacagcacga ggccgtcgat cggctccttg
     1681 accgaggtcg acgagaagac cgcgaccgag ccgccccgag ccgac
//
LOCUS       DAOOLN010000414         1723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_89347_length_1723_cov_3.815348, whole genome shotgun sequence.
ACCESSION   DAOOLN010000414 DAOOLN010000000
VERSION     DAOOLN010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89347_length_1723_cov_3.815348"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PLW80_05990"
     CDS             <1..795
                     /locus_tag="PLW80_05990"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB66089.1"
                     /translation="WKLLVFNDISGQKRDARDLAASNDELEFWVRQRTAALRQEVAQR
                     TAAENRLRELNEEMAKTQKEILWTLSEVVESRSRETAYHVIRVGEYARILSKAYGLSA
                     EEVDIITAAAPMHDVGKIAIPDSILSKPGLLTDDEREVMKRHTVIGKEILGTSTRPLL
                     RAAAIIALEHHERWDGKGYPLGKVAADISLSGRIVSLCDVFDALFTKRVYKDAWPLEK
                     VLALFKEESGGAFEPRLVDILFEHIDDFIDVTKRFKDEPAPALPLA"
     gene            856..>1723
                     /locus_tag="PLW80_05995"
     CDS             856..>1723
                     /locus_tag="PLW80_05995"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HPB66090.1"
                     /translation="MSIKKAVSLVAAILAVASAWPSPSSSPSSSPSSAVVDLTAVDLT
                     EAVRLDASWAIYWNRFVDPAAFEVGEAPLPDAYVPMPALWTSYGPGFDSVGYATLQLS
                     LVGLEPGRRYGLKASAFQSAASLYAGGRLVQAHGRPARTAADEEPAWRSLVSPLEADE
                     TGRLELVLHVSNFSDRSGGTHASLAFGDLAAVARERERAVAYELFMCGAILVMGAYYL
                     GLFAFRRKERAALWFGLLCVTLAVRVLLYDEFYLVSIFPSLGFRTLFALGYLSFTAAV
                     ACAAGRREGYVDT"
BASE COUNT          260 a          596 c          575 g          292 t
ORIGIN      
        1 tggaagctgc tcgtgttcaa cgatatctcc ggccagaagc gcgacgcccg cgacctggcg
       61 gccagcaacg atgagctgga gttttgggtc aggcagcgga ccgcggcgct caggcaggag
      121 gtcgcccaac gtaccgccgc cgagaaccgc ctgcgcgagt tgaacgagga gatggccaag
      181 acccagaagg agatcctctg gaccctgtcg gaggtggtgg agagccgtag ccgcgagacc
      241 gcctaccacg tgatccgcgt cggcgagtac gcgaggatac tctccaaggc ctacgggctg
      301 tccgcggagg aggtcgacat catcacggcc gcggcgccga tgcacgacgt gggcaagata
      361 gcgatccccg actcgatact gtccaagccg ggcctcctga ccgacgacga gcgcgaggtg
      421 atgaagcgcc acaccgtcat cggcaaggag atactcggca cgtcgacgcg ccccctcttg
      481 cgcgccgccg ccatcattgc gctcgagcac cacgagcgtt gggacggcaa gggctatccg
      541 ctcggcaagg tggccgccga tatcagtctg tccgggcgta tcgtctcgct ctgcgacgtg
      601 ttcgacgcgc tgttcaccaa gcgcgtgtac aaggacgcct ggccgctcga gaaggtattg
      661 gccctgttca aggaagaatc cggcggcgcc ttcgaaccgc gcctcgtcga catcctgttc
      721 gagcacatcg acgacttcat cgacgtgacg aagcgcttca aggacgagcc ggccccggcg
      781 ctgcccttgg cctgaccggg cgccgggccg gcgttcgttg cgaagcgact cggcaggtga
      841 tatagtcgat ccccgatgag catcaaaaaa gccgtatccc tagtcgcggc gattctcgcg
      901 gtcgcgtccg cctggccgtc gccatcgtcg tcgccatcgt cgtcgccatc gtcggccgtc
      961 gtcgacttga cggcggttga cctgactgaa gccgtgcgcc tcgacgcctc ctgggccatc
     1021 tactggaacc gcttcgtcga tccggcggca ttcgaggtcg gggaggcgcc gctacccgac
     1081 gcctacgtac cgatgcccgc cctctggacg agctacgggc cgggcttcga ctcggtcggc
     1141 tacgcgaccc tgcagctgag cctcgtcggg ctcgagcccg gccggcgcta cgggctcaag
     1201 gcctcggctt tccagagcgc cgcgtcgctc tacgcgggcg gtcgcctggt ccaggcccac
     1261 ggccggcccg cgcgtacggc cgccgacgag gaaccggcct ggcgctcgct cgtctcgccg
     1321 ctcgaggccg acgagacggg gcgcctcgag ctcgtgctgc acgtgtcgaa ttttagcgac
     1381 cgctcgggcg gcacccacgc cagtctcgcg ttcggcgacc tggccgccgt cgcgcgcgag
     1441 cgtgagcggg cggtggccta cgaactgttc atgtgcggcg ccatcctggt gatgggggcc
     1501 tactacctgg gcctcttcgc ctttcggcgc aaggagcgcg cggctctctg gttcgggctc
     1561 ctgtgcgtca cgctcgccgt ccgcgtgctc ctgtacgacg agttctacct cgtctccatt
     1621 ttcccctcgc tcggcttcag gaccctgttc gcgctcggct acttgagctt caccgcggcg
     1681 gtcgcctgcg cggcggggag gcgggagggg tacgtcgaca cgc
//
LOCUS       DAOOLN010000415         1718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_89767_length_1718_cov_2.707757, whole genome shotgun sequence.
ACCESSION   DAOOLN010000415 DAOOLN010000000
VERSION     DAOOLN010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89767_length_1718_cov_2.707757"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PLW80_06000"
     CDS             <1..834
                     /locus_tag="PLW80_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPB66091.1"
                     /translation="TADIVIDTEDGSTLFIRETIDESYTYRTYPTVRLKGFILHFHRG
                     SLPGDRDRVVATITGAPLSSPSSAPPSASAGATGTPSAGATGTPSASAGATGTPTARA
                     SPGSSASAGATGTPSAGASPGSSAPGSSAPGTGVPVPGGAVAASPSPASQPGGAFELA
                     RGERGVVMLLYDLRFVADGDELLASERGRLDAIAEALGRLPDRTFLVEGHTADLGKPA
                     GQYELSERRARRIVDELAARGIPENRFVYRGLGADRPIAPNDSEANRARNRRVEITIL
                     D"
     gene            878..1492
                     /locus_tag="PLW80_06005"
     CDS             878..1492
                     /locus_tag="PLW80_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66092.1"
                     /translation="MDLRRYLGLYAVSPLGLAMSAASVAVGAAVGFAASPLAGVAACL
                     VTEATAFAITTASGLGPKAAVAEGERRAWAEASERLEAVKRDRDRLATMRLPDDELGS
                     LVRLAATRGASYLAASRNAKSRVPQAEEALSDCLSVADLYLKELDAASTERRHGLDDA
                     DPFADAKGRAKAALVDKAAIIEKATRDLSGGLSSADAMDIKERL"
     gene            1489..>1718
                     /locus_tag="PLW80_06010"
     CDS             1489..>1718
                     /locus_tag="PLW80_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66093.1"
                     /translation="MSSSSTRRAYGRFHWKPSLAALLLLLAALAVGSASALASDLASV
                     DITVNLRPDGRAEIFYTLEWRVSSGDMSGFYF"
BASE COUNT          262 a          611 c          585 g          260 t
ORIGIN      
        1 accgccgata tcgtcatcga caccgaggac ggctccacgc tgttcatccg cgagacgatc
       61 gatgagagct atacctatcg aacgtatccg accgtcaggc tcaagggttt catcctgcat
      121 ttccatcgcg gcagcttacc cggggaccgg gatcgggtcg tcgcgacgat caccggcgcg
      181 cccttgtcgt cgccatcgtc agcgccaccg tcagcgtccg ccggcgcaac cgggacgccg
      241 tcggccggag ccaccgggac accgtcggcg tccgccggtg ccaccgggac gccgacggcc
      301 agagcctcgc caggctcgtc agcgtccgcc ggcgcaaccg ggacgccgtc ggccggagcc
      361 tcgccaggct cgtcggcgcc aggctcgtcg gcgccaggaa ccggagtgcc tgtaccgggc
      421 ggcgccgtcg cggcgagccc gtcgccagcg agccagcctg gaggggcgtt cgagctggcc
      481 cggggcgagc gaggcgtcgt gatgttgctg tacgatctgc gcttcgtggc cgacggagac
      541 gagctgctcg cctccgagcg cggccgactg gacgcgatag ccgaggcgct cggacgccta
      601 cccgatcgta ccttcctcgt cgagggtcat accgccgacc ttggcaaacc ggccggccag
      661 tacgagcttt cggagcggag ggccaggcgc atcgtcgacg aactggccgc gcggggcatc
      721 ccggagaatc ggttcgtcta tcgcgggctc ggtgccgatc ggcccatcgc gccgaatgac
      781 agcgaggcca accgcgcgcg caatcgcaga gtcgagataa cgattctcga ctgagggtag
      841 gcttgatact gacttggcgc ggcgctacag tatcgccatg gatctacgac gataccttgg
      901 cctatacgcc gtctccccgc tcggtctcgc catgtcggcg gcctcggtcg ccgtcggggc
      961 ggccgtcggc ttcgccgcct cgccgctcgc cggcgtggcc gcctgcctcg taaccgaggc
     1021 gaccgccttc gcgataacca cggcgagcgg cctcggcccc aaggccgccg tcgccgaggg
     1081 tgagcgccgc gcctgggccg aagcgagcga gcggctcgag gccgtcaagc gcgaccgcga
     1141 ccgcctcgcg acgatgcgcc tgcccgacga cgagctgggc tcgctcgtca gactcgccgc
     1201 gacgaggggc gcctcctacc tggcggccag ccggaacgcc aagagccgcg tcccccaggc
     1261 cgaggaggct ttgtccgact gcctttcagt cgccgacctg tacctcaagg aactggacgc
     1321 ggcctcgacc gagaggaggc acggccttga cgacgccgat cccttcgccg acgccaaggg
     1381 acgcgcgaag gcggccctgg tcgataaggc ggccatcatc gagaaggcca cgcgcgacct
     1441 gtcgggcggc ctttcgtccg ccgacgccat ggatattaag gagcgactat gagcagtagc
     1501 agcactcgcc gcgcgtacgg gcgcttccac tggaagccga gcctggcggc gcttctcctc
     1561 ttactcgcgg ccctggccgt cggtagcgcc tcggcgttgg cgagcgacct ggcgtcggtc
     1621 gacattacgg tcaacctgcg gcccgacggc agggccgaga tcttctatac gctcgagtgg
     1681 cgcgtgtcgt cgggtgatat gagcggcttc tatttcca
//
LOCUS       DAOOLN010000416         1716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_89929_length_1716_cov_2.957857, whole genome shotgun sequence.
ACCESSION   DAOOLN010000416 DAOOLN010000000
VERSION     DAOOLN010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89929_length_1716_cov_2.957857"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1716
                     /locus_tag="PLW80_06015"
     CDS             <1..>1716
                     /locus_tag="PLW80_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPB66094.1"
                     /translation="YRGVDGLDIGVVDVPGHERYLRNMVAGAWGLDLVVLVVAADDGW
                     MPQTGLHAAIVASMAEAAVVIAVTKADAVAPGRAATVALEAEDRATALFGVRPPSVAV
                     SSVSGDGITELKSAIDSRLASLPPLEPMAARLFVDRSFSPRGGGVVVTGSLSGGPVGV
                     GDEVLRWPSGDRLRVRGIQSYRESVSSAEPNCRAALSVAAPKGGFARGDLLAAPGAAV
                     LSGDEFIVRLLPLPGSGSLSAVDAKGKPALRPGVMVEFAVGSARRDAELWPIGGTGFA
                     RLTLSEPVACPLGTPFALLRLGGAELIGRGVAVRSGKTKVDDRKALLAALPRAAAAAD
                     AMTDAGWGIRGAVALALAIETLVRGWAAAPVGFPARAAEEAGLASAGPGGTTAPTGRT
                     APTGTTAPTGRTTLFDPGRLSAFGKAAAAKAGEPGGLQRAMAESLFASVFGAGKAAPG
                     RAQAVEAAMASLEASGAVVRNGSAWSTPGAEKRLTPADSALLGRLEAAGKAGLEPGKD
                     CPQTDARGLKALCSLGLAMPLDGGIFFSKAVYDACMVAILRGLGPGCRFSVPEAKARS
                     GLSRK"
BASE COUNT          216 a          615 c          621 g          264 t
ORIGIN      
        1 ggtaccgggg tgtcgacggc ctggacatcg gtgtcgtcga tgtacccggc cacgagcgct
       61 atctgcgcaa catggtcgcc ggcgcctggg gtctggacct cgtcgtgctc gtcgtcgcag
      121 ccgacgacgg ctggatgcct cagaccggcc tccacgcggc catcgtcgcc tcaatggccg
      181 aggcggccgt cgtcatcgcc gtgaccaagg cggatgccgt ggcgccggga cgagcggcca
      241 cggtcgcgct cgaggccgag gatcgggcga cggctctgtt cggcgtccgc ccgccgtccg
      301 tcgcggtgtc cagcgtctcc ggcgatggta ttacggaact caagtcggct atcgacagcc
      361 gcctggcctc cctacccccg cttgagccga tggccgccag gctcttcgtc gaccggtctt
      421 tcagccctcg cggcggaggc gtcgtggtca ccggctcgct atcgggcggc cccgtcggcg
      481 tcggcgacga ggtactacgg tggccgagcg gcgatcgcct cagggttcgc ggcatccaga
      541 gctaccgcga gagcgtgtcc agcgcagagc cgaattgcag ggcggcgcta tccgtcgccg
      601 cgccaaaggg gggcttcgcc cgcggagacc tcctcgccgc tccaggggcc gccgtgctat
      661 cgggagacga attcattgtc cggctccttc cgctacccgg ctcgggctcg ctatcggcgg
      721 tcgacgccaa gggtaagcca gccctgcgcc ccggcgtgat ggtcgagttc gccgtcggta
      781 gcgccaggcg cgacgcggag ctatggccca taggcggcac gggcttcgcc cggcttacgc
      841 tgtcggagcc ggtcgcctgc cccctcggca cgccgttcgc gctcctgagg ctcgggggcg
      901 ccgagctcat aggccgcggc gtggctgttc gctccgggaa gaccaaggtc gacgatagga
      961 aggcgctttt ggccgcgctt ccgcgcgccg ccgccgcggc agacgcgatg accgacgccg
     1021 gctggggcat ccgcggcgcc gtggccctag ccctggctat cgagacgcta gtacgtggct
     1081 gggcggccgc tccggtagga tttccagcca gagcggctga ggaggctggg ctggcctccg
     1141 ccggcccggg cggcacgacc gcgcctactg gcaggaccgc gcctactggc acgaccgcgc
     1201 ctactggcag gaccaccctg ttcgaccccg gccgactctc ggccttcggc aaagccgcag
     1261 ccgcgaaggc tggcgagccc ggggggctcc agcgggccat ggcggagtcg ctattcgcct
     1321 cggtattcgg cgccggcaag gcggctccgg gacgcgctca agccgtcgag gccgcgatgg
     1381 cctcgctcga ggcgagcggc gccgtcgtac ggaacggctc ggcttggtcg acgcccgggg
     1441 ccgagaagcg tctcacgccg gctgactccg ccttgcttgg ccgtctcgag gcggcgggca
     1501 aggcggggct cgagccgggc aaggactgtc cccagaccga cgcacgcgga ctcaaggcgc
     1561 tctgctcgct cggcctggcc atgcccctcg acgggggaat cttcttctcc aaggccgtct
     1621 acgacgcctg catggtcgcc atactccggg gcctgggccc gggctgccgt ttctcggtgc
     1681 cggaggccaa ggcgcgctcg ggcctctcgc ggaagt
//
LOCUS       DAOOLN010000417         1716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_89936_length_1716_cov_2.833233, whole genome shotgun sequence.
ACCESSION   DAOOLN010000417 DAOOLN010000000
VERSION     DAOOLN010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89936_length_1716_cov_2.833233"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PLW80_06020"
     CDS             complement(<1..378)
                     /locus_tag="PLW80_06020"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HPB66095.1"
                     /translation="MTVQRFLAPVPGLALAGALATVAYVAGKAAPIIGGPVFALAFGI
                     LVGLAPRPACFDSGQRLASKKTLQVAVALLGFGMDFGRALSVGSSSIVVMLSTLLASF
                     ATAFLVSRALRVRGKTVGLIAIGT"
     gene            502..>1716
                     /gene="purB"
                     /locus_tag="PLW80_06025"
     CDS             502..>1716
                     /gene="purB"
                     /locus_tag="PLW80_06025"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPB66096.1"
                     /translation="MSDHSVYQSPFSWRYGSQSMRELWSDERKRRLWRSIWVSLARVE
                     SEYGLVTSAQVDELAAHAEELDVARSLEIEADIRHDLMAEVRAFAERCPTAGGVIHLG
                     ATSMDIEDNADALRLRDALGLVVGSLDRLLAALADKIDEYAGLPVMAFTHIQPAEPTT
                     LGYRLAVWAQDLLDDRERIARERDAVRGKGFKGAVGSSASYAELVGPENVGRFEERLA
                     EELGLGFYPIATQTYTRKQDYAVVSALAGLGASLHKFALDLRILQSPPIGELSEPFGK
                     HQVGSSAMPFKRNPINAEKIDSLARSLSVMPQVAWQNAAQSILERTLDDSANRRSMLP
                     EAFLICDEILATATRIVTGLVVSEEAIGRTMAAYGPFAATERVLMAMGKAGADRQEGH
                     ERLRRHALGGGEG"
BASE COUNT          305 a          594 c          569 g          248 t
ORIGIN      
        1 ggtacctatc gctatcagcc cgactgtctt accccggacc cgcagcgctc tcgagaccag
       61 gaaagccgtc gcgaacgatg cgaggagcgt cgatagcata acgacgatcg acgacgagcc
      121 tacgctcagg gcccgcccga agtccatgcc gaatccgagc agcgctacgg ctacctgtag
      181 cgtttttttg gacgccagcc gctggccgga atcgaagcag gccggcctcg gggccagccc
      241 taccaggatg ccgaaggcca gcgcgaacac cggcccgccg atgatcggcg cggccttgcc
      301 ggcgacgtac gctaccgtcg ccaaggcacc ggccagcgcg agccccggaa cgggtgccaa
      361 gaatcgttgt accgtcatgc cgagagtgta acgaaatacg atacataaaa aaataccatt
      421 atagccgagg atgacctggt catcggatcg agacccggcg acccgctagc ccctcaggcc
      481 gtcctctgct actatccgcc catgagcgac cattcggtgt accagtcacc gttcagctgg
      541 cgctacggta gccagtccat gcgcgagctg tggagcgacg agcgcaagcg ccgcctctgg
      601 cgctccatct gggtcagcct ggccagggtc gagtcggagt acggactggt cacctcggcc
      661 caggtcgacg agctggccgc ccacgccgag gagctcgacg tcgcccgctc cctcgaaatc
      721 gaggcggaca tacgccacga cctgatggcc gaggtccggg ccttcgccga gcgctgcccg
      781 acggccggcg gcgtcatcca cctcggggcg accagtatgg acatcgagga caacgccgac
      841 gccctgcgcc ttcgcgacgc cctcggcctg gtcgtcggtt ctcttgaccg gctgctcgcc
      901 gcgctggcgg acaagatcga cgagtacgcc gggctaccgg taatggcctt cacgcatatc
      961 cagccggccg agccgacgac gctcggctac cggctggcgg tctgggccca ggacctgctc
     1021 gacgaccggg agcggatcgc ccgcgagcgg gacgcggtac gcggcaaggg cttcaagggc
     1081 gcggtcggca gctccgcgtc gtacgcggag ctggtcggcc ccgagaacgt cggccgtttc
     1141 gaggagcggc tagccgagga gctcgggctg ggcttctacc cgatagccac gcagacctac
     1201 acgcgcaagc aggactacgc ggtcgtctcg gcgctggccg gcctcggcgc ctcgctccac
     1261 aaattcgcgc tcgacctgcg tatcctgcag tcgccgccga taggcgaatt gtccgagccg
     1321 ttcgggaagc accaggtcgg ctcgtcggcg atgccgttca agcgcaaccc gatcaatgcg
     1381 gagaagatcg actcgctggc ccgctcgctg tcggtgatgc cgcaggtggc ctggcagaac
     1441 gcggcccagt cgatactgga gcgcaccctc gacgactcgg ccaaccgccg ctcgatgctg
     1501 cccgaggcct tcctgatctg cgacgagata ctggcgaccg cgacgaggat cgtgacgggc
     1561 ctcgtcgtct ccgaggaggc gatagggcgg acgatggccg cctacggccc cttcgccgcc
     1621 accgagcgcg tgctgatggc gatgggcaag gccggcgccg accgacagga aggccacgag
     1681 cggctacgca ggcacgcgct cggtggcggc gaaggg
//
LOCUS       DAOOLN010000418         1716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_89946_length_1716_cov_2.614088, whole genome shotgun sequence.
ACCESSION   DAOOLN010000418 DAOOLN010000000
VERSION     DAOOLN010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89946_length_1716_cov_2.614088"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PLW80_06030"
     CDS             <1..670
                     /locus_tag="PLW80_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66097.1"
                     /translation="YEGRDGAGGVVAGNGGPSSSRAVAAALATVAAVESAKPSPADAE
                     MAPSAGRAPVGQPRPVASPSELLLNWIQDLESSLADEAARADSAEREILALRSALAEA
                     RAFEAFNHGLASDGAAETGASGAPGEMVPGSSGTLASDLMVDVVAELRRLSDAEPLIG
                     MSDLRTRALLRAIVRDPAIRAEYPELFDSLDTYLERVEADAHQRGRAAAYEDILERLG
                     ATGR"
     gene            complement(890..1267)
                     /locus_tag="PLW80_06035"
     CDS             complement(890..1267)
                     /locus_tag="PLW80_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66098.1"
                     /translation="MALAISSLAVACDEFSFVSLLSLPDQATPDELSLTVAQDSVPAG
                     SSVALEVSGGVPPYTFKPLSGSDLYQPTSSRSLGTISDSVFTAGQAIGKILITVEDAV
                     GYLRFRLLSYSRPLRFLCNPLSE"
     gene            complement(1332..>1716)
                     /locus_tag="PLW80_06040"
     CDS             complement(1332..>1716)
                     /locus_tag="PLW80_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66099.1"
                     /translation="HNPLASVRLGVQGLARDIRNGRCTPGEIGSYLDLIDNEIDKCIA
                     VTHRLLMLSRTPEEAPQVVDLALALDDGSTLARAEPFVDAGVQAGVPLGRDFFLGLDA
                     RFVVVFDPEMLIMGTSPTITLFKEI"
BASE COUNT          305 a          521 c          604 g          286 t
ORIGIN      
        1 ctatgaaggc cgcgacggag cgggcggcgt cgtagcaggc aacggagggc cctcgtctag
       61 ccgggccgtc gcggcagccc tggcaaccgt cgccgcggtc gagtcggcca agccttcgcc
      121 ggccgacgcc gagatggcgc cgagtgccgg ccgcgccccc gtaggccaac ctcgtccggt
      181 agcgagcccc tccgaactgc tcctgaactg gattcaggac ctggagtcgt cgctggcgga
      241 cgaggcggcg cgggcggata gcgccgagcg cgagatactg gccttgcgtt cggccctggc
      301 cgaggctcgg gcgttcgaag ccttcaatca cggcttagct tccgacggcg ctgctgaaac
      361 cggcgcatct ggcgctccgg gagagatggt tcccggttcg tccggaacgc tagcgtccga
      421 cctgatggtt gacgtggtag ccgagcttag gcgcctgtcc gacgccgagc cgcttatcgg
      481 catgtccgac ctgcgcacgc gcgcgctttt gcgagccatc gtccgcgacc cggccatccg
      541 agccgagtac cccgagctgt tcgactcgct cgatacgtac ctggaacgcg tcgaggcgga
      601 tgcccaccag cgcggccgcg ccgccgccta cgaggatata ctggagcggc ttggggctac
      661 tggaagataa ttgttacggg catggactcg tatgtgcccg agagagcgaa gagcctgaac
      721 cctaacgtcg ccgccgctgg ttgttggttg aaggtggcgg acacgctatc cgcatcgtac
      781 gaagctagga acgagtattc ggagccatcg atagaccgct cgagccgata accattgatc
      841 atggcgacgt caggatagga ccagtaaagt tgtaccgtcg tccctcctcc tattcgctta
      901 gaggattgca taaaaaacgg agtgggcggg agtacgacaa tagacgaaaa cgtagatacc
      961 ccactgcgtc ctccacggtg atcagtattt tcccgatagc ctggccggcg gtaaagacgc
     1021 tgtccgagat ggtgccgagg ctacggctac tggtcggctg gtacaggtcg gaaccggata
     1081 gcggcttgaa ggtatacggg gggacgccgc cgctcacctc gagggcgacc gagctgccgg
     1141 cgggcacgct gtcctgggcg accgtcagcg acagctcgtc aggcgtggcc tggtcgggca
     1201 gggacaggag cgacacgaac gagaattcgt cacaggcgac ggccagggag gagattgcga
     1261 gagccatgag tgcgatcggc gcgatcgggg acagaggatt gatctgtttc gggggcgtgc
     1321 gcttcatacg gctagatttc cttgaacagc gtgatggtgg ggctggtgcc catgatgagc
     1381 atctcggggt cgaaaacgac gacgaatcgg gcgtctaggc ccaggaagaa gtcgcgcccg
     1441 agcgggacgc cggcctggac gcccgcgtcg acgaagggct ccgcccgcgc cagggtcgag
     1501 ccgtcgtcga gggccagggc caggtccacc acctggggcg cttcctccgg cgtgcgcgac
     1561 agcatcagca ggcggtgggt gaccgcgatg cacttgtcga tctcgttgtc gatcaggtcc
     1621 aggtagctgc cgatctcgcc gggtgtgcag cgcccgtttc ggatgtcccg ggccaggccc
     1681 tggacgccca ggcgcaccga ggccaggggg ttgtgg
//
LOCUS       DAOOLN010000419         1714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_90104_length_1714_cov_2.522001, whole genome shotgun sequence.
ACCESSION   DAOOLN010000419 DAOOLN010000000
VERSION     DAOOLN010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90104_length_1714_cov_2.522001"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="PLW80_06045"
     CDS             <1..659
                     /locus_tag="PLW80_06045"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPB66100.1"
                     /translation="KELDARDILLKEIHHRVKNNLQVVSSLLSLESDCVEDERAKSVF
                     VDCQTQVQSMSLVHEQIYRGSSLDGVEASAYFARLAEYLAGVHDARNRGILLLVERTD
                     LVLSIDTAMPLSIIATELVSNSFKHGYPDGSGGRVSIALAKDGDEYAFSVSDDGAGFR
                     TGYRGHGEPPPAGKNKGIGMDLVEALAAQLGGRMEQGSPPAGAVTTVRFPVRDAAQDR
                     "
     gene            complement(634..>1714)
                     /locus_tag="PLW80_06050"
     CDS             complement(634..>1714)
                     /locus_tag="PLW80_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66101.1"
                     /translation="ADAARADAAMAAGLEAIAAAEALAASVAGQYELGAALLAAMHAR
                     LSEYRLMESRLDRALLEGRYNCVGSSTLYAIMASAAGLRVRGVLLDDHAYCYLETAGG
                     RVDVETTIPGGYDARKGEPPAGAADVSVRGVIALALRNRATTLERSGQWDGALALAVD
                     AYAYAPDPSSFSTLVGRVNNAVAYRIRSGAYGEALGLAEAAQLAYGPDRRLDEIRRSA
                     ALAAATEALRVAKPDEALALADGVAASGIADEAWLERAYAYAYAGLAERLRAAGDHLG
                     AWALASEAVARFPAYGALSSLERTARINWARAEHNRFAALYNAGAYGEALAVVRAALA
                     LAPGDAMLLADEKAAAEAIGPGQRP"
BASE COUNT          258 a          609 c          611 g          236 t
ORIGIN      
        1 gcaaggagct cgacgccagg gacatcctgc tcaaggagat ccaccacagg gtcaagaaca
       61 acctgcaggt agtctcgagc ctcctgtcgc tcgagagcga ctgcgtcgag gacgagcggg
      121 ccaagtccgt cttcgtcgac tgccagacgc aggtacagtc gatgtcgctg gtccacgagc
      181 agatataccg cggctcgtcg ctcgacggcg tcgaggcaag cgcctatttc gcgaggctcg
      241 cggagtacct cgccggggtg cacgacgcga ggaaccgcgg catcctgctc ctcgtcgagc
      301 ggaccgacct cgtgctgtcg atcgatacgg cgatgccgct gtcgatcata gcgaccgagc
      361 tggtatcgaa ctcgttcaag cacggctacc ccgacggttc gggtggccgc gtaagcatag
      421 ccctggccaa ggacggcgac gagtacgcgt tctcggtatc ggacgacggc gccggcttcc
      481 ggaccgggta tcgcggccac ggcgagcccc ctccggccgg caagaacaag ggcataggca
      541 tggacctggt cgaggccctg gcggcccagc taggcggccg catggagcag ggctctccgc
      601 cggcgggcgc cgtcaccacg gtccgattcc ccgtcaggga cgctgcccag gaccgatagc
      661 ctcggcggcc gccttctcgt cggccagcag catcgcgtct cccggggcca gggcgagggc
      721 cgccctcacg acggccaggg cctcgccgta ggctccggcg ttgtacagcg ccgcgaagcg
      781 gttatgttcg gccctcgccc agttgatcct ggccgtccgt tccagcgagg agagggcccc
      841 gtacgctggg aaacgcgcta ccgcctcgga agccaaggcc caggcgccga gatggtcgcc
      901 cgcggcccgt agccgctcgg cgaggccggc gtaggcgtag gcataggcgc gctcgagcca
      961 ggcctcgtcg gcgataccgg aggcggccac cccgtcggcc agggcgaggg cctcgtcggg
     1021 cttcgcgacc cgtagcgcct cggtcgccgc ggcgagggcc gcgcttcggc ggatctcgtc
     1081 caggcgtcgg tccgggccgt acgccagctg ggccgcctcg gccaggccca gggcttcgcc
     1141 gtaggcaccg ctccgtatac ggtacgccac cgcgttgttg acccttccca cgagggtcga
     1201 gaacgatgac gggtcgggcg cgtaggcgta ggcgtcgacc gccagagcga gcgccccatc
     1261 ccattgcccg gagcgctcga gcgtcgtcgc gcggttacgc agggccagcg cgatgacgcc
     1321 gcggaccgac acgtcggccg cgccggcggg cggctcgccc ttgcgggcat cgtaaccgcc
     1381 ggggatggtg gtctccacgt cgacgcggcc gccggccgtc tcgaggtagc agtaggcatg
     1441 gtcatcgagc agcacgcccc ggacgcgcag gcctgcggcg ctcgccataa tggcgtacag
     1501 cgtcgacgag ccgacgcagt tataccgccc ctcaagcagc gcgcggtcca ggcgcgactc
     1561 catgagccgg tactcggata ggcgcgcatg catggcggcg agcagcgccg ccccgagctc
     1621 gtactggccg gccaccgagg cggccagggc ctcggcggcc gctatcgctt ctagtccggc
     1681 cgccatggcg gcgtcggcgc gcgcagcgtc ggcg
//
LOCUS       DAOOLN010000420         1712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_90235_length_1712_cov_3.340978, whole genome shotgun sequence.
ACCESSION   DAOOLN010000420 DAOOLN010000000
VERSION     DAOOLN010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90235_length_1712_cov_3.340978"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLW80_06055"
     CDS             complement(<1..295)
                     /locus_tag="PLW80_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HPB66102.1"
                     /translation="MNGAKEAKAGAKTVRKATKAPAKAKKTPEKPTLVIVESPAKAKT
                     IEKYLGSSYMVLASMGHLIDLPKSRLGVDVESGFAPEYLTIRGKAKLLKELQKA"
     gene            complement(349..1353)
                     /gene="dprA"
                     /locus_tag="PLW80_06060"
     CDS             complement(349..1353)
                     /gene="dprA"
                     /locus_tag="PLW80_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPB66103.1"
                     /translation="MAAELASLRDMIVARMPFLSARQRIKLVMRVGGAPLCDLCLEDV
                     ERAVGHRLDGARWDPDAWEREAERDLRFLSAVGGSSYGYFDAGYPAILRETARPPFML
                     YVRGRLPDGRRPSVAIVGTRYPTGRGLDAALALAMEAAAEGVQVVSGLARGIDSAAHR
                     GAIAVGGRTYAVLGRGIDAVYPASNKELAARMVEAGGGIASEYPPGTPPSRWTFPERN
                     RILAGLCRSTVVVEAPGGSGALISAAFALEEGRDVYVAAACSGGPRSAGSDALAADGA
                     VTLSSFGDIIEDWRCGVGRHGAFEPGGFDARSRRSGNRRRRAPLRRPSRTRLTSPAYG
                     "
     gene            complement(1343..>1712)
                     /locus_tag="PLW80_06065"
     CDS             complement(1343..>1712)
                     /locus_tag="PLW80_06065"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB66104.1"
                     /translation="AAAQPGGGAASRAEWLAAAVGAYRRSIEIEPRNTKALYGLAVLY
                     TFELEDHESAIGPLETLLGIETKNVDAMFVYARALYGSGRLQEASDVYDRIISTTRVD
                     EKRKQAADNKKRVLDELYGG"
BASE COUNT          259 a          628 c          539 g          286 t
ORIGIN      
        1 ccgccttctg gagttccttc aggagcttcg ccttgcctcg tatcgtcagg tactcgggcg
       61 cgaaaccgct ctccacatcg acgccgaggc gagacttggg caggtcaatc aggtgcccca
      121 tcgacgcgag caccatatag gacgaaccga ggtacttctc gatcgtcttg gccttggcgg
      181 gcgactcgac gatgaccaga gtcggctttt cgggcgtctt cttggccttg gccggggcct
      241 tcgtggcctt gcggaccgtc ttagctccgg ccttggcttc cttcgcgccg ttcatgctgt
      301 ccatcccagc gctctcatcg ctcgccatcg ttacgttctc ctactcgttc acccgtacgc
      361 cgggctcgtc agcctagtcc tgctcggtcg ccgtagcggc gccctgcgtc ggcgattgcc
      421 ggatcgcctc gaacgcgcat cgaagccgcc gggctcgaac gcgccatggc gcccgacgcc
      481 gcaacgccag tcctcgatga tatcgccgaa cgacgatagc gtcaccgccc cgtcggcggc
      541 caaggcgtcg gaaccggccg acctcggccc gcccgaacag gcggccgcca cgtagacgtc
      601 gcggccttcc tcgagggcga aggcggcgct gatcagcgct cccgaaccgc cgggcgcctc
      661 gaccaccacg gtcgaccggc ataatccggc cagtatccgg ttccgttccg ggaacgtcca
      721 gcgcgaaggc ggcgtgccgg gcggatattc tgacgcgatc ccgcccccgg cctcgaccat
      781 cctcgcggcc agctccttgt tcgacgcggg atataccgcg tctatgccgc ggccgagcac
      841 ggcgtaggtc cgcccgccga ccgcgatagc cccgcgatgc gccgccgagt cgatgccccg
      901 ggccaggccc gataccacct ggacgccctc ggcggcggct tccatcgcca gcgccagggc
      961 cgcgtcgagg ccgcggccgg tcggatagcg cgtaccgact atggcgaccg acggtcggcg
     1021 cccgtccggc aggcgaccgc gaacgtatag catgaacggg ggcctggccg tctcccgtag
     1081 gatcgccgga tagccggcat cgaagtagcc gtacgacgaa ccccctacgg ctgagaggaa
     1141 gcgcagatcc cgctcggcct cgcgctccca ggcgtccggg tcccagcggg ctccgtcgag
     1201 cctatggcct acggcccgct cgacgtcctc caggcacagg tcgcataggg gagcgccccc
     1261 gacgcgcatg acgagcttaa tgcgctgcct agccgacagg aacggcatcc ttgccactat
     1321 catgtcccgt agcgaagcca gctcagccgc catagagctc atccagaacg cgtttcttgt
     1381 tgtcggcggc ctgcttcctc ttctcgtcta ccctggtcgt cgagatgatg cggtcgtaca
     1441 catcggaggc ctcctggagc cggcccgagc cgtacagggc gcgcgcgtag acgaacatcg
     1501 cgtcgacatt cttcgtctcg atgccgagca gggtctcgag cggcccgatg gcggactcgt
     1561 ggtcttcgag ctcgaaggtg tacagcaccg ccaggccgta taacgccttg gtattgcgag
     1621 gctctatctc gatcgagcgc cgatacgcgc cgacagcggc cgcgagccac tcggcgcgcg
     1681 aggctgcccc gccgcccggc tgagccgccg cg
//
LOCUS       DAOOLN010000421         1711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_90325_length_1711_cov_2.496377, whole genome shotgun sequence.
ACCESSION   DAOOLN010000421 DAOOLN010000000
VERSION     DAOOLN010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90325_length_1711_cov_2.496377"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="PLW80_06070"
     CDS             complement(<1..868)
                     /locus_tag="PLW80_06070"
                     /inference="COORDINATES: protein motif:HMM:NF016827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HPB66105.1"
                     /translation="MQFQRPSIIQEQRQKLSPQMIQSIRLMALPLQELKEEIQQEIEA
                     NPALEVLEDRSTMSLESIPEERESESEAVQRFENSSDPGYSRSGDDDSDAKRMFLEGA
                     ITYPETLQEHLLWQLRLQPITPSQREIGERLVQNLNADGFHKEDPYLLCKGFSKATVD
                     KVMAVIHRLEPSGACTRDFRESLLVQAEQAPGAPHGALEIIGDHMDLLERGRHQDIQK
                     RLKLDDDELEAALGFIKGLNPFPGRLYSAEETKYVTPDVQVKLKDGEFVIVINDEEIP
                     VIGINPFFDRLSG"
     gene            1145..>1711
                     /locus_tag="PLW80_06075"
     CDS             1145..>1711
                     /locus_tag="PLW80_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPB66106.1"
                     /translation="MSKHKHQDIPETQNEGQVTDQSRERTAGSPSQATPDSGADAVEA
                     RVKAAEAKAAAAEAKAVELEAEVSSLKDQYLRKLADYENFRKRMFREKEDAVQYANSQ
                     ILVDLVAVLDDFERAIKSSELSRDFSSLHDGVGMIQKNLLSTLAGKYGLTHFESVGAA
                     FDPNVHEAVMSEPGDCPEPTVVEEFIKGY"
BASE COUNT          330 a          495 c          547 g          339 t
ORIGIN      
        1 cgccggatag acggtcgaag aacgggttga tgccgatgac gggtatctcc tcgtcgttga
       61 tgacgatgac gaactcgccg tccttcagct tgacctgaac gtccggggtg acatacttgg
      121 tctcctcggc cgagtagagc ctgccgggga aggggttcag ccccttgatg aagcccaggg
      181 ccgcctcgag ctcgtcgtcg tcgagcttga ggcgtttctg gatgtcctga tgcctgcccc
      241 gctcgagcag gtccatgtgg tcgccgatga tctcgagcgc cccgtgcggc gcgccggggg
      301 cttgctcagc ctggacgagg agcgattccc taaaatccct ggtgcaggcg ccggagggct
      361 ccagcctgtg gatgacggcc atcaccttgt ccacggtcgc cttggagaaa cccttgcaga
      421 gcaggtaggg gtcctcttta tggaagccgt cggcgttcag gttctggacg aggcgttcgc
      481 ctatctcacg ctgggagggg gtgataggct ggagcctgag ctgccacagg aggtgttcct
      541 gaagcgtctc ggggtaggtg atcgccccct cgaggaacat gcgtttggcg tccgagtcgt
      601 cgtcgccgga acggctatag cccgggtcgg agctgttctc gaagcgctgg acggcctccg
      661 actccgattc gcgctcctcg ggaatggact cgagcgacat cgtggaccga tcctcgagca
      721 cttcgagggc tggattcgcc tcgatctcct gttgtatctc ttccttcagc tcctggaggg
      781 gtagggccat gagcctgatg gactggatca tctggggact gagcttctgc ctctgctcct
      841 ggatgatcga tggtctctgg aactgcaagt acgatccttt cgtgacggcg atgcgtgacg
      901 cggggcgact accctcaaga taaatggtat agcatcgtcg gctaaagtca agccgaacga
      961 ctgtaagccc ggtggcccga acctacaaga accgtgccta ttttcacgat tattcgtcta
     1021 agaatgccgt aagtggcttg acgctttaaa atcaccctta ttactttaca tgatactagc
     1081 cgcacggctc ccagcgtcaa gcgcgtacga agaccctggg gtatcatatt cgtagagcga
     1141 ggccatgtct aaacacaagc accaggatat accagaaacg cagaacgagg gccaggttac
     1201 cgatcaatcg cgggagcgga cggccggaag cccttcccag gcgacgcccg atagcggcgc
     1261 cgatgccgta gaggccaggg tcaaggccgc cgaggccaag gctgcggccg ccgaggccaa
     1321 ggccgtcgaa ctggaggccg aggtttcgtc tctcaaggac cagtacctgc gcaagctggc
     1381 ggattatgag aatttccgca agcgcatgtt cagggaaaag gaggacgcgg tccagtacgc
     1441 caactcccag atcctcgtcg acctcgtggc cgtgctcgac gatttcgagc gggccattaa
     1501 gagctccgag ctgtcgaggg atttctcgtc gctgcacgac ggggtcggca tgatccagaa
     1561 gaacctgctg tcgacgctgg cgggcaagta cgggctgacc catttcgagt cggtcggcgc
     1621 cgcgttcgac ccgaacgtgc acgaggccgt gatgagcgag ccgggcgact gccccgagcc
     1681 gaccgtcgtc gaggaattca taaagggcta c
//
LOCUS       DAOOLN010000422         1710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_90409_length_1710_cov_2.425378, whole genome shotgun sequence.
ACCESSION   DAOOLN010000422 DAOOLN010000000
VERSION     DAOOLN010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90409_length_1710_cov_2.425378"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(313..777)
                     /gene="ybaK"
                     /locus_tag="PLW80_06080"
     CDS             complement(313..777)
                     /gene="ybaK"
                     /locus_tag="PLW80_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HPB66107.1"
                     /translation="MTTNALRLVRSMGISVEAFEYEVDESDLSAVSVASRLGLDPDRV
                     FKTIAVRGAKVGPFLCVVPGSTEVDLKKAAKAAGDKSAEPLPLRELEPLTGYVRGGCS
                     PVGTRTPLPVFIDETAALWETISVSAGARGLQMVLAPDDLARASGAEFADLT"
     gene            complement(786..>1710)
                     /locus_tag="PLW80_06085"
     CDS             complement(786..>1710)
                     /locus_tag="PLW80_06085"
                     /inference="COORDINATES: protein motif:HMM:NF020772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HPB66108.1"
                     /translation="AVWSETNGFPADPVYRDFIRDVAATDAVWSETNGFPADPVYRDF
                     YRDIGFDLPLDYIGPYVEQQQIRTFTGFKYWAITGPGDRKVPYDAERASLRVEDHVES
                     FLRDRVLQAKAAAAHMGGREPLMVAPYDAELFGHWWYEGPQWIETLFRLAHHTDELQF
                     VTLSEYMRANPENFESVPEFTSWGDGGYGGVWLEKSNDWIYRHVFKMIERMTELAERF
                     PNESGLRERVLNQAAREVLLAQASDWPFLLRAGKSGSFARKQIEDAVTNFNRIYEMLC
                     ANTVGTEWLTRLEKRNNIFPSINYRVFRRKR"
BASE COUNT          318 a          535 c          544 g          313 t
ORIGIN      
        1 ggaggacgtc gtcgcggccg tacgcgccta cgtcgtggac gcgcctatca gctggatagt
       61 cgtaggcgac caggcgacgc tgtccaaagt cgataagacc aaattcgacg ccttcacagg
      121 caccgtggag taagtcgaga atagggcaat cagaccacgg aggcacggag gcacggaggc
      181 gcggagaata caagaggaga atagagaatg agaaacgcaa ggattataat ctccttgttt
      241 tcttaatctc gcctcattcc ttcgctttct ccgtgtctcc gcgtctccgt ggttcaattc
      301 gaaactaaaa gctcaggtga ggtcggcgaa ctcggcgccg gaggcgcgcg cgaggtcgtc
      361 cggagccagc accatttgca gtccccttgc accggcgctg accgagatcg tctcccatag
      421 cgcggccgtc tcgtcgatga acaccggcag gggcgttctg gtgcctacgg gcgagcagcc
      481 gccgcgaacg tagccggtca gtggctcgag ttccctgagc ggcagcggct ccgccgattt
      541 gtcgccggcg gccttggccg ccttcttgag gtcgacttcg gtcgagcccg gcaccacgca
      601 gaggaagggt ccgaccttgg ctccgcggac ggctatcgtc ttgaacacgc ggtcggggtc
      661 aaggccgagc ctcgaggcga ccgatacggc cgacaggtcg gactcgtcga cctcgtactc
      721 gaaggcctcg acactgatgc ccatggatcg gaccagcctg agggcgttgg tcgtcactgg
      781 ttcacctagc gtttacgccg gaagacccgg tagttgatac tcggaaagat gttattgcgc
      841 ttctcgagcc tggtcagcca ttcggtgccg acggtgttgg cgcagagcat ctcgtagata
      901 cggttaaaat tggtcaccgc gtcctcgatc tgcttcctcg caaaggagcc ggacttgccg
      961 gctcgcagca ggaagggcca atccgaggcc tgggccagca acacctcgcg agccgcctgg
     1021 ttgagcacgc gctcgcgcaa cccgctctcg ttcggaaagc gctcggcgag ctcggtcatg
     1081 cgctcgatca tcttgaacac atgccgatag atccagtcgt tcgacttctc gagccagacg
     1141 ccgccgtagc cgccgtcgcc ccacgacgtg aactcgggaa ctgactcgaa gttctcgggg
     1201 tttgcccgca tatactccga cagcgtgacg aactgtagct cgtcggtgtg gtgggccagc
     1261 ctgaacagcg tctctatcca ctgcggtccc tcgtaccacc aatggccgaa cagctcggcg
     1321 tcgtacggag cgaccatcag cggctcgcgg ccccccatgt gggcggcggc cgccttggcc
     1381 tgtaggacgc ggtcgcgcag gaagctctcg acgtggtcct cgacgcggag cgaggcgcgc
     1441 tcggcgtcgt acggaacctt ccggtcgccg ggcccggtga tggcccagta cttgaagccg
     1501 gtgaaggtac ggatctgctg ctgctcgacg tacggcccga tatagtcgag cggcaggtcg
     1561 aaaccgatgt ccctgtagaa gtcgcgatat accggatccg ccggaaagcc gttcgtctcc
     1621 gaccagacgg cgtcggtggc cgccacgtcg cggatgaagt cgcgatatac cggatccgcc
     1681 ggaaagccgt tcgtctccga ccagacggcg
//
LOCUS       DAOOLN010000423         1710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_90419_length_1710_cov_1.954683, whole genome shotgun sequence.
ACCESSION   DAOOLN010000423 DAOOLN010000000
VERSION     DAOOLN010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90419_length_1710_cov_1.954683"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="PLW80_06090"
     CDS             <1..1143
                     /locus_tag="PLW80_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPB66109.1"
                     /translation="RQRYWGEPIPLVHCPKCGVVPVPEDQLPLTLPEVSSYEPSGTGE
                     SPLAKIDDWVDTTCPTCGGPARRETNTMPQWAGSCWYYLRYLDPSNPGTFVGRDKVDY
                     WMPVDLYVGGVEHAVLHLLYSRFWHKVLFDLGLVNTAEPFQRLVNQGMILGEDNQKMS
                     KSRGNVINPDDIVREYGADALRLYEMFMGPLEVSKPWMTAGLVGVTRFLERMYALGEK
                     PVVDAGPSAELLKLTHKTVRKVDADTASLNFNTAISAMMVLSSELARLDSLPRAAYEP
                     LVLMLAPYAPHLAEELWEKLGHRESLVRAAWPSYDPALCEDDEDTIVVQVNGKLRGDF
                     KAAKGAGKDALEASAKALAKVAEQLVGKDVIKVVVVPGKLVNFVVR"
     gene            complement(1236..>1710)
                     /locus_tag="PLW80_06095"
     CDS             complement(1236..>1710)
                     /locus_tag="PLW80_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66110.1"
                     /translation="TTERAGLGPTAFAALLAAASLALTTLRGRLKPLAAAFDAACAAS
                     ALVVGAAILLFWLCARYDEAAWNLNLLWAGALPLVATILGGRRLPAKARSAAGALYAV
                     SAACAALVAVAGGFGFQTILPATRLVALALALRWADRAGALGAAARLLRRRSRPE"
BASE COUNT          288 a          566 c          604 g          252 t
ORIGIN      
        1 cgccagcgtt actggggcga gcccatcccc ctggtccatt gcccgaagtg cggcgtcgta
       61 cccgtgcccg aggaccagct gccgctgacc ctgccggagg tctcgagcta cgagccgtcg
      121 gggaccggcg aaagcccgtt ggccaagatc gacgactggg tcgatacgac ctgccctacc
      181 tgcggcggac cggccaggcg cgagaccaac acgatgcccc agtgggccgg ttcctgctgg
      241 tattacctgc gttacctgga tccgtcgaac ccgggcacct tcgtcggccg cgacaaggtc
      301 gactactgga tgcccgtcga cctctatgtc ggcggtgtcg agcacgcggt gctccatctg
      361 ctctactcgc gcttctggca caaggtcctc ttcgacctgg ggctcgtcaa cacggccgag
      421 ccgttccagc gcctggtcaa tcagggcatg atcctgggtg aggacaacca gaagatgtcc
      481 aagagccgtg gcaacgtgat caatcccgac gacatcgtgc gggagtacgg cgccgacgcg
      541 ctcaggctct acgagatgtt catggggccg ctcgaggtgt caaagccctg gatgacggcc
      601 ggcctcgtcg gcgtgacgcg ctttctcgag cggatgtacg cgctcggcga gaagccggtc
      661 gtcgacgcgg ggccgtcagc cgagctactc aagctgacgc ataaaaccgt gcgcaaggtc
      721 gacgctgata ccgccagcct gaacttcaac acggccatct ccgcgatgat ggtgctgtcc
      781 tccgagctcg ccaggctcga ctcgttgcca cgggcggcct acgaacccct cgtgctcatg
      841 ctcgccccct acgctccgca cctcgccgag gagctctggg aaaagctcgg gcaccgtgag
      901 agcctcgtca gggccgcttg gccgagctac gacccggccc tctgcgagga cgacgaggat
      961 acgatcgtcg tgcaggtcaa cggcaagctg cgcggcgact tcaaggccgc caagggcgcg
     1021 ggcaaggacg cgcttgaagc ctccgccaag gctctcgcca aggtggccga gcaactagtc
     1081 ggcaaggatg tcatcaaggt cgtcgtcgtg cccggtaagc tggtcaattt cgtggtcagg
     1141 taggcgggcg ccagtccggg agcgcgccgg ttcggggcgc gcctaccggt atcgagcgac
     1201 gatgggccgc gggtagtcgt tacccgcggc ctttattatt ccggccggga tcgacgacgc
     1261 aggagcctcg ccgccgcgcc gagagcgccg gcccggtcgg cccatcgcag ggccagagcg
     1321 agcgcgacga ggcgcgtcgc cggtaggata gtctggaacc cgaagccgcc ggcgactgcg
     1381 acgagcgcgg cgcaggccgc cgagaccgcg tagagcgctc cggccgcgga tcgcgccttg
     1441 gcaggcaggc gtcggccgcc gagtatcgtc gcgacgagtg gtaaggcgcc cgcccagagc
     1501 aggttgaggt tccaggcggc ctcgtcgtag cgggcgcaca gccagaagag gaggatagcg
     1561 gctccgacga cgagcgccga cgcggcgcag gccgcgtcga aggccgcggc gagaggcttg
     1621 agccggccgc ggagggtggt aagggctaac gaggcggcgg cgagcagcgc ggcgaaggcg
     1681 gtcggaccga ggccggcgcg ctcggttgtc
//
LOCUS       DAOOLN010000424         1706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_90723_length_1706_cov_2.462750, whole genome shotgun sequence.
ACCESSION   DAOOLN010000424 DAOOLN010000000
VERSION     DAOOLN010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90723_length_1706_cov_2.462750"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PLW80_06100"
     CDS             complement(<1..390)
                     /locus_tag="PLW80_06100"
                     /inference="COORDINATES: protein motif:HMM:NF021210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2400 family protein"
                     /protein_id="HPB66111.1"
                     /translation="MARRLDALYDEYNDPRWIEPDPLAVVRRYDDPADVELAGLAAAA
                     LALGNAALIVRAAEAALAPLGPRPASALAEMDDEDLAASLASFRYRFFDGGDLASLLA
                     AARNLRRRHGGLEAAFLAGDDPESGDYV"
     gene            complement(432..>1706)
                     /locus_tag="PLW80_06105"
     CDS             complement(432..>1706)
                     /locus_tag="PLW80_06105"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB66112.1"
                     /translation="ALAVFRIKTTDSAGATLAAVERFNRFGLPSAAAELARGIALERS
                     GDLQGAYSAYARTEEAANDCYPATLGGARMLVGLGRGEEALEVASRLDARIVESVAGR
                     RVKAMALYQAKRWAEALPLITAVLLDDPLDSRFALMRAHLLIERGEYKQAAPLLDAYA
                     SINPDDRLYILLRARIAMESAKDRSQASAALRRGLTRYPSDPELTLYAAELFAGGDPA
                     ERAEALALAQKAYDADPSSIRALRLLLGFDLAAGDYTAAAAKADAVLASGMAFGDTEA
                     LYRSYRGAGRSVDALALARSWRAKEPGSEAAAVALATSMVDLGQKAAASELIGTLLAS
                     KGTPAYRSTLYWLQSRLQPGDEAALSSLRSALVENGMNIDALAAMSDIYVRRGDYQRA
                     RFYLKQAIAISPDRADIVERRNVLAQLGVAIP"
BASE COUNT          245 a          594 c          625 g          242 t
ORIGIN      
        1 gacgtagtcg ccgctctccg ggtcatcgcc ggccaggaag gccgcttcca ggccgccgtg
       61 gcgccgccgt aggttcctgg cggccgccag cagcgaggcc aggtctccgc cgtcgaagaa
      121 gcggtagcgg aagctcgcca gcgaggccgc cagatcttca tcgtccatct cggccagggc
      181 gctcgccgga cgcggcccga ggggcgccag cgccgcctcg gccgcccgga cgatcaacgc
      241 ggcgttgccg agcgccaggg ccgccgccgc gaggccggcc agctcgacgt ccgcggggtc
      301 gtcgtagcgc ctgactacgg cgagcgggtc gggttctatc catcgggggt cgttgtactc
      361 gtcgtacagg gcgtcgagac ggcgggccaa tgtcgcgtcg ggacagcccg aggcccggat
      421 aggctcggcg ctcagggtat cgccacgccc agctgggcca gcacgttgcg ccgctcgacg
      481 atgtcggctc ggtccgggga tatggctatc gcctgcttca ggtagaagcg cgcgcgctgg
      541 tagtcgcccc tgcgcacgta gatgtccgac atggcggcca gggcgtcgat gttcatgccg
      601 ttctcgacca gggccgagcg tagggacgac aacgccgcct cgtcgccagg ctggagcctg
      661 gactggagcc agtacagggt cgaccggtag gccggcgtac ccttcgaggc caggagcgtc
      721 cctatcagct cggacgcggc cgccttctgg ccgaggtcga ccatcgaggt ggccagcgcg
      781 acggccgccg cctcggagcc gggttccttc gcgcgccacg agcgggccag ggccagcgcg
      841 tcgaccgagc gaccggcgcc gcggtaggag cggtatagcg cctcggtatc gccgaaggcc
      901 atcccggagg ccagtacggc gtcggccttg gccgcggcgg ccgtataatc cccggccgcc
      961 aggtcgaagc ccaggagcag ccggagcgcc cgaatcgacg acggatccgc gtcgtaggcc
     1021 ttctgggcca gggccagggc ctcggcccgt tcggccgggt ctccgccggc gaacagctcc
     1081 gcagcgtaga gagtcagttc cgggtcggac ggatagcgcg tcagcccccg gcgcagggcg
     1141 gccgaggcct gactccggtc cttggcgctc tccatcgcta tgcgcgcgcg gagcagtata
     1201 tagaggcgat cgtcggggtt gatgctcgcg tatgcgtcga gcaacggcgc ggcctgcttg
     1261 tactcacccc gttcgatcaa aaggtgcgcg cgcatcaggg cgaagcgcga gtcgaggggg
     1321 tcgtcgagca gcaccgcggt gatcagcggc aaggcctcgg cccagcgctt ggcctggtag
     1381 agggccatag ccttgacgcg acggccggcg accgactcga ctatcctcgc gtcgagcctg
     1441 gacgcgacct cgagcgcctc ctcgccgcgg cccagcccga cgagcatccg ggcgccgccc
     1501 agtgtggccg ggtagcaatc gttggccgct tcctccgtcc gggcataggc cgagtaggcg
     1561 ccctgcaggt cgcctgatcg ttccagggct atcccgcggg ccagctcggc ggcggccgag
     1621 ggcaggccga agcggttgaa acgctcgacg gcggctagcg tcgcgccggc cgaatcggtc
     1681 gtcttgatcc taaagaccgc cagcgc
//
LOCUS       DAOOLN010000425         1706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_90729_length_1706_cov_2.259843, whole genome shotgun sequence.
ACCESSION   DAOOLN010000425 DAOOLN010000000
VERSION     DAOOLN010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90729_length_1706_cov_2.259843"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PLW80_06110"
     CDS             <1..594
                     /locus_tag="PLW80_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66113.1"
                     /translation="DETVSPSASRAASARAASARAASARAAILGIVVVLAGAAMADAL
                     LIPGLSASSASRREAAMDEIAAATDDEAMIRAAKAAGAIALRHDGRTLLAATGPISPS
                     RYAVLSRGGLEAWFDVSDEARARGTAAAIAALASLVAAAGYAATLYGLSPLRALARRG
                     TAPTRPDQTAFDGPGAPRRDAPAGREELAGILGKRAP"
     gene            591..881
                     /locus_tag="PLW80_06115"
     CDS             591..881
                     /locus_tag="PLW80_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HPB66114.1"
                     /translation="MIVSMLQVIIEIPESFSLKDKRRVVKSVKDRLVSKFRVSCAETD
                     LQESLRFAQLGAALVSNSAEFGEGVMRKALAVIEGELSLRLHDSGIHSERFD"
     gene            932..1534
                     /locus_tag="PLW80_06120"
     CDS             932..1534
                     /locus_tag="PLW80_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66115.1"
                     /translation="MKRSTLLLAAATAILVSSCIGIDADARIAADGSVELTLAYTVSA
                     AVDELGKLGANADYLPLPVGRNDLELAASRAGGELRSWSREDGAESFAIKASLRFPSV
                     ASFAAFLDPAGEGAAYAEAGGRSTLTIRLSEGFDPADDDMLEFVKLAFADYAVNLRIT
                     APRTPTATSGLALSGRVATFSMKAADLYASNSPVTISLSW"
BASE COUNT          262 a          621 c          557 g          266 t
ORIGIN      
        1 gatgagacgg tgagcccaag cgcttcgcgc gcggcgtcgg cgcgcgcggc gtcggcgcgc
       61 gcagcgtcgg cgcgcgcggc gatcctaggc atcgtcgtcg tgctggcggg cgccgccatg
      121 gccgacgcat tactcatacc cggcctttcg gcgagttcgg cgtcgcggcg cgaggccgcg
      181 atggacgaga tagcggctgc gaccgacgat gaagctatga tccgggccgc caaggccgcc
      241 ggcgcaatcg ccctgagaca cgacggccga acgctcttgg ccgcgaccgg accgatctct
      301 ccatccaggt acgccgttct gtcgcgcggc ggtctcgagg cctggttcga cgtatcggac
      361 gaagcccgcg cccgcggaac cgcggccgcg atagccgccc tggccagcct cgtcgccgcc
      421 gccggctatg cggcgacgct ctacggcctt tcgccgctcc gggcgctggc acgtcgcggg
      481 acggctccta cgcgccccga ccagacggcg ttcgacgggc caggcgctcc tcgccgcgac
      541 gccccggccg gcagggagga gctcgccggc atcctcggca agcgcgcccc atgatcgtct
      601 ccatgctcca ggtcatcatc gagatacccg agtcgtttag cctcaaggac aagcgtcgcg
      661 tggtcaagtc ggtcaaggat aggctcgtgt ccaagttccg cgtctcgtgc gcggagaccg
      721 acctgcagga atcgctacgc ttcgcccagc tgggcgccgc gctcgtatcg aactcggctg
      781 aattcggcga gggggtcatg cgcaaggcac ttgccgtcat cgagggcgag ctgtcgctcc
      841 ggctccacga ctccgggatc cacagcgagc gcttcgacta gcgtgacggc gcgctcgaca
      901 acggggcgat tttgagctac ggttgtctat catgaagcga agtaccctat tgctcgccgc
      961 cgctacggcc atccttgtat catcgtgcat cggcatcgac gccgacgccc gcatcgccgc
     1021 ggacggctcc gtggagctga cgctcgccta taccgtgtcg gccgccgtcg acgagctcgg
     1081 caaactcggc gccaacgccg actacctgcc gctgccggtc ggcaggaacg acctcgagct
     1141 ggccgcctcg cgggccggcg gagaactacg atcctggtcg cgcgaggacg gcgccgagtc
     1201 gttcgcgatc aaagcctcgc tacgcttccc gagcgtggcc tccttcgcgg ccttcctcga
     1261 ccccgccggc gagggcgccg cctacgccga ggcaggcggc cgttcgactc tgacgatcag
     1321 gttgtccgag ggcttcgatc cggccgacga cgatatgctc gaattcgtca agctcgcctt
     1381 cgccgactac gccgtcaacc tgagaattac cgcacctcgg acgccgacgg ccacgagcgg
     1441 cctagcgctg tcggggcgcg ttgccacctt ctccatgaag gcggccgacc tgtacgcctc
     1501 gaactctcca gtaacgatca gcctgagctg gtaggagcga tgccatgaac gtatccaaaa
     1561 cgtatgccct gatcctcgcg ctaaccctct cggcggccct ttcgtcctgc gtctcgacgc
     1621 cggcgacaga cgcccgcgac gccgaccagg cgacgcccct cgagggaggc gcgccggcgg
     1681 ccccagccgg cgagaccagc tcgacg
//
LOCUS       DAOOLN010000426         1702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_91041_length_1702_cov_3.098361, whole genome shotgun sequence.
ACCESSION   DAOOLN010000426 DAOOLN010000000
VERSION     DAOOLN010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91041_length_1702_cov_3.098361"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PLW80_06125"
     CDS             complement(<1..288)
                     /locus_tag="PLW80_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66116.1"
                     /translation="MAGAGRTFAPSSGYSVGPFEGVTMAQARKLSGRSGEYADAYQVL
                     LDHAQVEPWAKTRASRRWGESARPKPGDLAVFVSKGASTPFFEHVALCRGGG"
     gene            complement(292..1083)
                     /locus_tag="PLW80_06130"
     CDS             complement(292..1083)
                     /locus_tag="PLW80_06130"
                     /inference="COORDINATES: protein motif:HMM:NF014649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66117.1"
                     /translation="MIDLKEYEFWFVVGSQDLYGDQVLKTVDEHSRKMVQSLDQRLPC
                     RLVWKPVVLGSDEILRLVRAANADPLCAGIVTWMHTFSPSKMWIAGLSELKRPLCHLH
                     TQFNRDIPLDSIDMDFMNLNQSAHGDREHGFIGARMRIPRKVVVGHWEDPAVVGRLSG
                     WMRAAAAFADGKALKVARFGDNMREVAVTEGDKVEAQIKLGWSVNGYGVGDLVESVAS
                     VREPDVDALVQAYGREYELAPEARSGKGAERVREQATARLGIRRR"
     gene            complement(1080..>1702)
                     /locus_tag="PLW80_06135"
     CDS             complement(1080..>1702)
                     /locus_tag="PLW80_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPB66118.1"
                     /translation="WLRHLLAWPARAGLLGSGVDPEAFEDSILPALESEAAAIDPARS
                     GVVALDWLNGRRTPDADQGLTGAIAGLSLGTDAPSLYRALIEAAAFGSRAIVERMRSE
                     GLPVERVVGIGGVARKSPLVMQISADVLGMDIAVSSSDQSCALGGAMFAACVAGLYPS
                     ALDAQASMRAPIERTYSPEPGCRAVYDALYAKYKALGAFAEKEMRK"
BASE COUNT          244 a          589 c          596 g          273 t
ORIGIN      
        1 ccctccgccg cggcacagtg cgacgtgttc gaagaacggc gtgctggcgc ccttgctcac
       61 gaagaccgcg aggtctccgg gcttgggcct cgcgctctca ccccagcgcc gcgacgctcg
      121 ggtcttcgcc cagggctcga cctgggcgtg atccagcagg acctgatagg catccgcgta
      181 ctcgccgctg cggcccgaga gcttgcgcgc ctgggccatg gtcacgccct cgaacgggcc
      241 gaccgagtag ccgctagacg gcgcgaaggt gcgtcctgcg ccggccatcg ctcagcgccg
      301 ccggatgccg aggcgggccg tggcctgctc gcgcacgcgc tcagcgccct tgccgctccg
      361 ggcctcgggg gccagctcgt actcgcgccc gtaggcctgt acgagggcgt cgacgtcggg
      421 ctcgcgaacc gaggctaccg actcgacgag gtcgccgacg ccgtagccgt tgaccgacca
      481 gccgagcttg atctgggcct cgaccttgtc gccctcggtg accgccacct cgcgcatgtt
      541 gtcgccgaaa cgggctacct tgagcgcctt gccgtcggcg aaggccgcgg cggcgcgcat
      601 ccatcccgat agcctgccga cgacggccgg gtcctcccag tggccgacca cgaccttgcg
      661 cggtatcctc atgcgcgccc cgatgaagcc atgctcgcgg tcgccgtggg ccgactggtt
      721 caagttcatg aagtccatgt ctatcgagtc gagcgggatg tcgcggttga actgggtgtg
      781 gagatggcag agcgggcgct tgagctcgga gaggccggct atccacatct tggacgggct
      841 gaaggtgtgc atccaggtga cgatgccggc gcagagcggg tcggcgttgg ccgcgcgcac
      901 gagcctgagt atctcgtcgg agccgagcac gacgggcttc cagacgaggc ggcagggcag
      961 cctctggtcg agtgactgga ccatcttcct cgaatgctcg tcgacggtct tgaggacctg
     1021 gtcgccgtag aggtcctggg agccgacgac gaaccagaac tcgtattcct tcaggtctat
     1081 catttcctca tctccttctc ggcgaaggcg ccgagggcct tgtatttggc gtagagcgcg
     1141 tcgtagacgg cgcggcatcc cggttcgggc gagtaggtcc gctctatcgg ggcccgcatc
     1201 gacgcctgag cgtcgagggc gctcggatag aggccggcga cgcacgcggc gaacatcgcg
     1261 ccgccgagcg cgcatgactg gtcgctcgac gatacggcta tgtccatccc gaggacgtcg
     1321 gcgctgatct gcatgacgag cgggctcttg cgcgccacgc cgcctatccc gacgacgcgc
     1381 tcgaccggca ggccctccga gcgcatcctc tccacgatgg ccctgctccc gaaggcggcg
     1441 gcttcgatca gcgcgcggta gagcgacggc gcgtccgtgc cgaggctgag cccggctatc
     1501 gctccggtca ggccctggtc ggcgtccggc gtgcggcgcc cgttgagcca gtcgagagcc
     1561 acgactcccg agcgcgccgg gtctatcgcg gcagcctcgg actcgagggc cgggagtata
     1621 ctgtcctcga aggcctcggg gtcgacgccc gatccgagga ggccggcccg ggccggccac
     1681 gcgagcaggt gcctgagcca gg
//
LOCUS       DAOOLN010000427         1702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_91063_length_1702_cov_2.551913, whole genome shotgun sequence.
ACCESSION   DAOOLN010000427 DAOOLN010000000
VERSION     DAOOLN010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91063_length_1702_cov_2.551913"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..1203
                     /locus_tag="PLW80_06140"
     CDS             220..1203
                     /locus_tag="PLW80_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HPB66119.1"
                     /translation="MRIGFCVPMNKAWSAAADGIEREHGEKVELVRGVEACLEAFPSL
                     DAVIANRVDASSYGAARSLKAVFVPFVGVNHLPADLLIERGVAVFNCHGNAESVAQHA
                     LSLALAGFGRLIEFHNDLRAETWHGFWVDKGSEDFWHSIYRKRCAILGAGAIGQKLAR
                     LLKAFDCTVIGYRRRPGEPMPECFDEVQGDLKAAVTGAEIVFVTLPLTDATRGLVGAA
                     ELEAMRGAFLVNVGRGDVVDERALYEALRDGVLAGAGIDVWYVYPPKGSVTGAPSRYP
                     IHRLPNVVMSPHVAGSTYEAVDLNIAQTVENLDRWLRTGDAEHRVDLRASY"
     gene            complement(1221..>1702)
                     /locus_tag="PLW80_06145"
     CDS             complement(1221..>1702)
                     /locus_tag="PLW80_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66120.1"
                     /translation="VTLLLDADDPERAARVARAARDLRKENPFCLWQIALRSDSGLPP
                     AGTVRRLADAFSMPEHYFELSRLFALDPQPDYQVRCFFVTGSEALALAALRERPDVET
                     VFTLGDAMPGAKLLEALPFLAFDREACPFELLYDVMSAYRDYPDLLIEAPRRLFGRE"
BASE COUNT          279 a          550 c          584 g          289 t
ORIGIN      
        1 agtcggctta ggcgacgata cgcatgacca gagcgtcaat gcgcatgccg ctatcaatag
       61 cgttctcttg gtttccacag tctattcctt ccacggtata gtgtatcgcg tcggaggtcg
      121 cggggcgata ggctttcttt ttatcatcgg aatcgcgtat cctatgcttg aaccactaga
      181 tcggcgtccg cgtagccggg cgccaacgga ggcgacagcg tgaggatcgg attctgcgta
      241 cctatgaaca aggcctggtc ggccgccgcc gacggcatag agcgggagca cggagagaag
      301 gtcgagctgg tacggggcgt cgaagcctgc ctcgaggcct tcccgagcct cgacgcggtg
      361 atcgcgaacc gtgtggatgc ctcgagctac ggggcggctc ggtcgctcaa ggccgttttc
      421 gtgcccttcg tcggcgtcaa ccatctcccc gccgacctgc tgatcgagcg cggcgtggcg
      481 gtgttcaact gccacggcaa cgcggagagc gtggctcaac acgcgctgtc gctggcgctc
      541 gcgggcttcg gcaggctgat cgagttccat aacgacctgc gggccgagac ctggcatggc
      601 ttctgggtgg acaagggcag cgaggatttc tggcattcca tctacaggaa gcgctgcgcc
      661 atactcggcg ccggtgcgat aggacagaag ctcgcgcgcc tgctcaaggc cttcgactgc
      721 acggtaatcg gctatcgccg taggcccggc gagcccatgc ccgagtgctt cgacgaggtg
      781 caaggcgacc tcaaagccgc cgtgaccgga gccgagatcg tcttcgtcac cctcccgttg
      841 accgacgcca ctcgcggcct cgtcggcgcc gccgagctcg aggcgatgcg cggggccttt
      901 ctggtcaacg tggggcgcgg cgacgtggtc gacgagcggg ctctctacga ggctctgcgc
      961 gacggcgtcc tggccggggc cggtatcgac gtctggtacg tctacccgcc caagggctcg
     1021 gtcacgggcg cgccctcgcg ctatccgatc cataggctgc cgaacgtggt catgtcgcct
     1081 cacgtggccg gctcgaccta cgaggctgtc gacttgaaca tagcccagac cgtcgagaac
     1141 ctggatcgat ggcttcggac cggggacgcc gagcaccgcg tcgacctgcg cgcatcctat
     1201 tgaaggctag cggcgtccgg ctactcgcgc ccgaatagcc ttcgcggcgc ctcgatgagc
     1261 aggtcggggt agtcgcgata ggcgctcatc acgtcgtaca gcagctcgaa ggggcaggcc
     1321 tcgcggtcga aggccaggaa gggcagggcc tcgagcagct tggcgccggg catcgcgtcc
     1381 ccgagcgtga agaccgtctc gacgtccggc cgctcgcgta gcgccgccag ggcgagcgcc
     1441 tccgagccgg tgacgaagaa gcacctgacc tggtagtccg gctgggggtc gagggcgaac
     1501 agccgcgaca gctcgaagta gtgctcgggc atcgagaacg cgtcggcgag gcgcctgacc
     1561 gtccccgctg gcggcagacc cgagtcggag cgtagcgcta tctgccagag gcagaacggg
     1621 ttctccttgc gcaggtccct ggccgccctg gccaccctgg ccgcgcgctc cggatcatcc
     1681 gcgtcgagga gcagggtgac cg
//
LOCUS       DAOOLN010000428         1695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_91562_length_1695_cov_2.837195, whole genome shotgun sequence.
ACCESSION   DAOOLN010000428 DAOOLN010000000
VERSION     DAOOLN010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91562_length_1695_cov_2.837195"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="PLW80_06150"
     CDS             complement(<1..882)
                     /locus_tag="PLW80_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB66121.1"
                     /translation="MNAIAIGEQLRTAREARGLTIDYVADETNIAKRYIAAMEEENFS
                     VFPGEPYIIGFLRNYAEYLGLESASIVQAFRGIRIQEQPAPIQELLQPKRARIWPFVA
                     VGLAAALGVATFLILRPGSDDVATDGPAMTRGELVQHRFDAAVLEKRLYPGDTLLVPY
                     GGDVYRIVLTGIDERVAIETPVGVSRFMLGEEGAIDLDKDNQAELYAFIVDFQKNDQS
                     RGAMIRFKAAAPLAEELPTAADQANAGAGVAGTQPATAPVVVALPTGAERPSTQDSII
                     FSGKRSPHPFVVNVTFRN"
     gene            complement(900..1571)
                     /locus_tag="PLW80_06155"
     CDS             complement(900..1571)
                     /locus_tag="PLW80_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HPB66122.1"
                     /translation="MKALPSIVLAALLAASPAMAQDILTASDFFKGVSERYASIKDYE
                     AAVEVKAGDQLMAGTVIYKAPSLMRMDFSKPASQVIVYDGQQLTIYVPELRAVLTQQT
                     SSSASAAAVTGEGLRMLGRNYSVTYETGPTPTPLPGAEGENVIRLVLSRLTVAEGFRT
                     ITLSIAPETRLIRRLEGVTLAGDLISYDFKAIKLDQDIPGTRFIYDSPASANMYNNFL
                     FSSDN"
BASE COUNT          310 a          511 c          553 g          321 t
ORIGIN      
        1 gttcctgaag gtcacgttga cgacgaaggg atgggggctg cgcttgcccg agaagatgat
       61 cgaatcctga gtcgaaggcc tctcggcccc ggtcggcaac gcgacgacaa cgggagccgt
      121 agcgggctgc gttccggcca cgccggcacc ggcgttggcc tggtcggcgg cggtcggcag
      181 ctcctcggcg agcggggcgg cggccttgaa gcgtatcatc gcgccgcgag actggtcgtt
      241 cttctggaag tcgacgatga aggcatacag ttcggcctgg ttgtccttgt cgaggtcgat
      301 cgcgccttcc tcgccgagca tgaagcgcga gacgccgacc ggcgtctcga tggccacgcg
      361 ctcgtcgatg cccgtaagga cgattcgata gacatcgcct ccgtagggca ccagcagggt
      421 atcgcccggg tacagacgtt tctcgaggac ggccgcgtcg aaccggtgct ggaccagctc
      481 gcccctcgtc atcgcgggcc cgtcggtcgc cacgtcgtcg gagcccggcc tcaggatcag
      541 gaacgtggcc acgccgagcg ccgccgctaa gcccacggcg acgaagggcc agatccgcgc
      601 gcgcttcggc tggaggagct cctggatggg cgccggctgc tcctggatgc gaatacccct
      661 gaacgcttgc actatagagg ccgactccag gccgaggtac tcggcgtagt tcctgagaaa
      721 accgattata tacggttcgc ctgggaaaac ggagaagttc tcttcctcca tagcggctat
      781 gtagcgctta gcgatgttcg tctcgtcagc gacatagtcg atggtaaggc cgcgtgcctc
      841 gcgagccgtt cgtagctgtt caccaatcgc gatagcgttc atggatacgt cgtcgctccc
      901 tagttatcgg aactgaataa gaaattgtta tacatattgg cggacgccgg cgaatcgtag
      961 atgaaccgcg ttcccggtat atcctgatcg agcttgatag ccttaaagtc gtacgagatg
     1021 aggtcgccgg ccagagtcac tccctccagc ctgcgtatca gccgcgtctc cggcgctatg
     1081 gacaacgtga tggtcctgaa gccctcggcc accgtgagcc tcgacagtac gagccggatc
     1141 acgttctccc cttccgcacc cggaagcggc gtcggggtag gcccggtctc gtaggtcacg
     1201 gaatagttcc tgccgagcat gcgcaagccc tcgccggtga cggccgccgc ggaggcggac
     1261 gagctcgtct gctgggtgag cacggcgcgt agttcgggaa cgtagatggt cagctgctgg
     1321 ccgtcgtaga cgataacctg cgaggccggc ttggagaaat ccatgcgcat cagcgacggt
     1381 gccttataga tcaccgtgcc ggccatcagc tggtcgccgg cctttacctc gacggccgcc
     1441 tcgtagtcct tgatcgaggc gtagcgttcg gagactcctt taaagaagtc gctggcggtg
     1501 agaatatcct gggccatcgc cggcgaggcg gcgaggaggg ccgctagaac gatcgaaggg
     1561 agagctttca cgtcgaataa cctaccgtgc gtcgtggatt cttcgatata gtatccgcta
     1621 gccggtaaaa tggcaagcga tgaggccgtc cgggccgagt atcgtcactc ggggagtccc
     1681 ttgagccgct cgagc
//
LOCUS       DAOOLN010000429         1694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_91658_length_1694_cov_2.478951, whole genome shotgun sequence.
ACCESSION   DAOOLN010000429 DAOOLN010000000
VERSION     DAOOLN010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91658_length_1694_cov_2.478951"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..737)
                     /locus_tag="PLW80_06160"
     CDS             complement(15..737)
                     /locus_tag="PLW80_06160"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HPB66123.1"
                     /translation="MKKFMRFAMAVALALQGCALLDAGDYASRAFMRESDPALAKDAF
                     PTMIKAAEAIQLADPDDQGAATLVASLYVMYANAFLDGEAFLLPDEAFEERLGLARRA
                     NALYLRAAALLAPFVEARAPGALGLAVVTEDPSLARLGKKDVPTLYWAAAAILAAFAS
                     DPMDFDNAARVSGALALFERARAVEPDWNGGALHELAISVYGSLPTELGGDRAKARAA
                     FELAKAASSASSPAATPVARAA"
     gene            873..>1694
                     /locus_tag="PLW80_06165"
     CDS             873..>1694
                     /locus_tag="PLW80_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HPB66124.1"
                     /translation="MLKEFKSLLPYLRRYKWHYALGLLSLVVVDAAQLLLPQYLRKAV
                     DSIAADGPRSIVAAIGASMVATAAAIAVGRFLWRYFIHGASRRIETELRDRLFARLMS
                     LPGSYFGEHAAGDIMARATNDMQAIRKATGMAFVAFVDGAFMSVAILAVMIAQNPGTT
                     LLTIAPLPLISVLIVVFGSMVGKRFERVQALYSGMSSVAQETFQGIRVVQSFVKEDEF
                     AKRFSEANDRYRAASMSLVMIYGFFFPLISFLAGLTTLVLMVAGGAAVIENRMTPG"
BASE COUNT          283 a          561 c          542 g          308 t
ORIGIN      
        1 gcggttggcg gccatcacgc cgccctggca acgggcgtag ccgcaggcga cgaggcgctc
       61 gacgctgcct ttgccagctc gaaggccgcc cttgccttgg cgcggtcccc gccgagctcg
      121 gtgggcaggg aaccgtacac agagatggct agctcgtgca acgctccgcc attccagtca
      181 ggctcgacgg ctcgggcccg ctcgaacagg gccagcgcgc ccgagactct ggcggcgttg
      241 tcgaagtcca tcgggtcgga cgcgaaggcg gccaggatag cggccgcggc ccaatacagc
      301 gtcggtacgt cttttttacc gaggcgggcc agggacgggt cctcagtgac gacggccaga
      361 ccaagggcgc cgggcgccct ggcctcgacg aacggcgcta gcagggccgc cgctcgcagg
      421 tacagcgcgt tggcccgcct ggcgagcccc aggcgctctt cgaaggcctc gtcgggcaat
      481 aggaacgcct cgccgtcgag gaaggcgttg gcgtacatca cgtagagcga cgccacgagc
      541 gtcgctgccc cttggtcatc gggatcggcg agttggatcg cttccgccgc cttgatcatc
      601 gtcggaaagg cgtccttagc tagggccggg tcgctctcgc gcatgaaggc gcgggaggcg
      661 tagtcgccgg catcgagaag ggcgcaaccc tgcagggcaa gcgcgacagc catggcgaaa
      721 cgcatgaatt tcttcatacg gatagtattt atgctcgtcg tctggctgtc aaccgcgttc
      781 gcttcagccc gggttaggat cctgtggata tcgcttgcgc cggagaaacg tgatacgttg
      841 aaacttgacg gaatacggta taccattctc cgatgctaaa ggaattcaag agcctgctcc
      901 cgtacctgag gcgttacaag tggcactacg cgctcggcct cctcagcctc gtcgtcgtgg
      961 acgccgcgca gttgctcctt ccccagtacc tgcgtaaggc cgtcgactcg atagcggccg
     1021 acggaccgcg atcgatcgtc gccgccatcg gcgcgtccat ggtcgccacg gccgcggcca
     1081 tcgccgtcgg gcgcttccta tggcgctatt tcatacacgg cgcctcgcgc cgcatcgaga
     1141 cggaactgcg cgaccgattg ttcgccaggc tcatgagcct acctggctcg tacttcggcg
     1201 agcacgccgc gggcgacatc atggccaggg ccacgaacga catgcaggcg atacgcaagg
     1261 ccaccggtat ggctttcgtc gccttcgttg acggggcctt catgtctgtc gccatactcg
     1321 cggtgatgat agcccagaac ccgggcacga ccctgttgac catcgcgcct ctgccgctga
     1381 tatccgtgct catcgtcgtc ttcggctcca tggtcggcaa gcgcttcgag cgcgtccaag
     1441 ccctgtactc gggcatgagt tcggtggccc aggagacgtt ccagggcata cgcgtggtcc
     1501 agtccttcgt caaggaggac gaattcgcca agcgcttctc cgaggcgaac gaccgctacc
     1561 gcgcggcctc gatgagcctc gtgatgatct acggcttctt cttcccgctc atttcattcc
     1621 tggccggact gacgaccctc gtgctgatgg tcgccggcgg cgccgccgtc atcgagaacc
     1681 gcatgacgcc aggg
//
LOCUS       DAOOLN010000430         1691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_91873_length_1691_cov_3.044621, whole genome shotgun sequence.
ACCESSION   DAOOLN010000430 DAOOLN010000000
VERSION     DAOOLN010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91873_length_1691_cov_3.044621"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PLW80_06170"
     CDS             <1..843
                     /locus_tag="PLW80_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66125.1"
                     /translation="DVGYYVSYHDGGLPIAIVWERNLAATPAEEYLPSDFPEALQQFV
                     YYVTFPVVELAGESGVASFSQLAKDLPKRYPSADGFYASEIDEEFPWYTPPEASYGYS
                     DGYDDDYGYYDDYGDEDYSDYDEDYVEGVESYGMIKVGSTVILGKHDDVNGGANWADE
                     MDAYVGTEAVVSSLPGADSQGFLVVRVNGNGYVWRVRNLTLQGRGEAGSYGYQVGDKV
                     IIGAHRYIDEDNNWASDMLEYVGRTATITTMEGTDGSECYIVHVDIDNGDWYWRVETM
                     QPAK"
     gene            957..>1691
                     /locus_tag="PLW80_06175"
     CDS             957..>1691
                     /locus_tag="PLW80_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HPB66126.1"
                     /translation="METLKRTVTCGALTGADAGKTVTLNGWVNRKRDHGGISFINLRD
                     RYGITQVVVDADAPESLKAIANELKSEYCVAVEGTVRARPDSMVNPDMATGQIEVVAS
                     NLVVLTKCDVLPFQIDDETDAKEELRLRYRYLDLRSGGMQRRIKLRNDVAFAVREHMI
                     GQGFYEIETPTFIKSTPEGARDYLVPSRLYPGKFYALPQSPQLYKQILMVSGFDKYFQ
                     IARCYRDEDARGDRQPEFTQIDIEMSF"
BASE COUNT          351 a          576 c          510 g          254 t
ORIGIN      
        1 gacgtcggct actacgtctc ctaccacgac ggcggcctgc cgatcgcaat cgtctgggaa
       61 cgcaacctcg cggccactcc ggccgaggaa tacctgccat cggacttccc cgaggctctg
      121 cagcaattcg tctactacgt cacgttcccc gtcgtcgagc tcgcaggcga gtcaggcgta
      181 gcctccttca gccagttggc caaggaccta cccaagcgct acccctccgc cgacggcttc
      241 tacgcctccg agatcgacga ggaattcccc tggtatacgc cgcccgaggc ctcctacggc
      301 tattccgacg gctacgatga cgattacggc tactacgacg actacggcga cgaagactac
      361 agcgactacg acgaggacta cgtcgagggc gtcgagagct acggtatgat caaggtcggc
      421 agcacggtga tcctcggcaa gcacgacgac gtcaatggcg gcgccaactg ggccgacgag
      481 atggacgcct acgtcggcac cgaggccgtc gtctcgtccc tgccgggagc cgacagccaa
      541 ggcttcctgg tcgtcagggt caacggcaac ggctacgtct ggcgcgtccg caacctgacg
      601 ctccaaggca ggggcgaggc cggcagctac ggctaccagg taggcgacaa ggtcataatc
      661 ggcgcccacc gctacatcga cgaggacaac aactgggcga gcgatatgct cgagtacgtc
      721 ggccgcacgg ccaccatcac gacgatggaa ggcaccgacg gctcggaatg ctatatcgtc
      781 cacgtcgaca tcgacaacgg cgactggtac tggcgcgtcg agacgatgca gccggccaag
      841 taaggccgcc atcccgattc cgaggagccc gccgcgaggc gggctccttc tatatacggc
      901 gcctatcgtc cctcccctgg acgaccggcc cggcgccgcg atatagtcgc gttactatgg
      961 aaacactcaa gcgcaccgtg acctgcggcg ccctgaccgg ggccgatgca ggcaagacgg
     1021 taaccctgaa cgggtgggtc aatcgcaagc gggaccacgg cggaatcagc ttcatcaacc
     1081 tccgcgaccg ctacggcatc acccaggtgg tggtggacgc cgacgcgccc gaatccctca
     1141 aggcgatagc gaacgagctc aagagcgagt attgcgtcgc ggtcgagggg acggtgcgcg
     1201 ccaggcccga cagtatggtc aaccccgaca tggccaccgg ccagatcgag gtcgtcgcgt
     1261 cgaacctcgt cgtactgacc aagtgcgacg tcctgccctt ccagatcgac gacgagacgg
     1321 acgcgaagga ggagctcagg ctccgctacc gctacctcga cctacgctcc ggcggcatgc
     1381 agcgccgcat caagctgcgc aacgacgtgg cgttcgccgt ccgcgagcac atgataggcc
     1441 agggcttcta cgagatcgag accccgacct tcatcaagag cacgccggag ggagcccgcg
     1501 actacctcgt gccgagccgc ctgtacccgg gcaagttcta cgcgctaccg cagagccccc
     1561 agctatacaa gcagatcctg atggtctccg gattcgataa gtacttccag atagcccgct
     1621 gctaccgcga cgaggacgcg cgcggcgaca ggcagcccga gttcacgcag atagacatcg
     1681 agatgtcgtt c
//
LOCUS       DAOOLN010000431         1691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_91895_length_1691_cov_2.359413, whole genome shotgun sequence.
ACCESSION   DAOOLN010000431 DAOOLN010000000
VERSION     DAOOLN010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91895_length_1691_cov_2.359413"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="PLW80_06180"
     CDS             complement(<1..849)
                     /locus_tag="PLW80_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPB66127.1"
                     /translation="MRDIQDFDSIMVRLASPDMIRSWSYGEVKKPETINYRTLRPEKD
                     GLFCERIFGTTKEWECYCGKFKSIRYKGVICDRCGVEVTHFKVRRERMGHIELASPVS
                     HIWFYRSVPSRMGLLLDLPVAALRSVLYYEKYIVIDAGDTDLKKMQLLTEEEYYEAQD
                     RYGGAFTAGMGAEAVRSLLEGIDLDDMAGELRAKMIEKGAKSDKRLLKRIEIIESFRG
                     SGNKPEWMIMDVIPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLQGLNA
                     PDIIIRN"
     gene            complement(1014..>1691)
                     /locus_tag="PLW80_06185"
     CDS             complement(1014..>1691)
                     /locus_tag="PLW80_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013944920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPB66128.1"
                     /translation="PYLDDGTPLDICLNPLGVPSRMNIGQLMETELGWIGSTLDEWFS
                     SPVFQSPDQEMIEAKLQEAGLPASCKTPVRDGRTGEHFQNPVTVGVIYFMKLHHLVDD
                     KMHARSTGPYSLVTQQPLGGKAQFGGQRLGEMEVWALEAYGAANTLQELLTIKSDDMT
                     GRAKVYEAIVKGEPHTAAGIPEAFNVMVQELRGLGLDMRIFDAKNKQIPLTERDEDLI
                     SKQGSNF"
BASE COUNT          279 a          515 c          551 g          346 t
ORIGIN      
        1 gttgcggatg atgatatccg gggcgttgag gccctggagg cgcttgaggc ggttattacg
       61 gttgatgacg cggcggtaca ggtcgttgag gtcgctcgtc gcgaagcgcc ccccgtcgag
      121 ctggaccatc ggcctgagct ccggcgggat gaccgggatc acgtccatga tcatccactc
      181 gggcttgttg ccggagccgc ggaagctctc gatgatctcg atgcgcttaa ggaggcgctt
      241 gtcgctcttg gcgcccttct cgatcatctt ggcgcgcaac tcgccggcca tgtcgtcgag
      301 gtcgatgccc tcgagcaggg agcgcacggc ctcggcgccc atgcccgcgg tgaaggcgcc
      361 gccgtagcga tcctgcgcct cgtagtactc ctcctcggta aggagctgca tcttcttgag
      421 gtcggtgtcg ccggcgtcta tgacgatgta cttctcgtag tacagcaccg agcgcaacgc
      481 cgcgaccggc aggtcgagga gcaggcccat gcgactcggc acggaacggt agaaccagat
      541 gtgcgagacc ggcgaggcca gctcgatatg gcccatgcgt tcgcggcgaa ccttgaagtg
      601 cgtcacctcg acgccgcagc ggtcgcagat cacgcccttg tagcggatcg acttgaactt
      661 gccgcagtag cactcccatt ccttggtcgt gccgaagatg cgctcgcaga agaggccgtc
      721 cttctcgggg cggagcgtgc ggtagttgat ggtctcgggc ttcttgacct cgccgtagct
      781 ccagctgcgg atcatgtccg ggctggccag gcgaaccatt atgctatcga aatcctgtat
      841 gtctctcatg cagtctcccc cttaaagcgg gctttgatcg gagaagaaga ccacggagac
      901 acggagacgc ggagatggaa ggaaagatat agagaaagcc gtttccctag tatccttgac
      961 tgatccttat ccatctcttc tccgtgcctc cgtgactccg tggctaattc ttgctagaag
     1021 ttcgaacctt gcttgctgat caggtcctcg tcgcgctcgg tcagcgggat ctgcttattc
     1081 ttcgcgtcga agatccgcat gtcgaggccg aggccgcgca gctcctgcac catgacgttg
     1141 aaggcctcgg gtatgccggc cgcggtgtgc ggctcgccct tgacgatggc ctcgtagacc
     1201 ttggcgcggc cggtcatgtc gtcggacttg atcgtcagga gctcctgcag cgtgttggcg
     1261 gcgccgtagg cctcgagggc ccagacctcc atctcgccga ggcgctggcc gccgaattgg
     1321 gccttgccgc cgagtggctg ctgggtaacc agcgagtacg ggccggtcga acgagcgtgc
     1381 atcttgtcgt cgaccaggtg gtgcagcttc atgaagtaga tgacgccgac ggtgaccggg
     1441 ttctggaaat gctcgccggt acggccgtcg cggaccggcg tcttgcacga cgcgggcagc
     1501 cccgcctcct ggagcttggc ctcgatcatc tcctggtcgg gcgactggaa caccggggag
     1561 ctgaaccact cgtccagagt cgatccgatc caccccagtt cggtctccat cagctggccg
     1621 atattcatgc ggctcggcac gccgagcggg ttcaggcaga tatcgagcgg ggtgccgtcg
     1681 tcgaggtacg g
//
LOCUS       DAOOLN010000432         1690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_91977_length_1690_cov_2.272783, whole genome shotgun sequence.
ACCESSION   DAOOLN010000432 DAOOLN010000000
VERSION     DAOOLN010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91977_length_1690_cov_2.272783"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLW80_06190"
     CDS             complement(<1..261)
                     /locus_tag="PLW80_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HPB66129.1"
                     /translation="MRVEYINPFVESAFNVMKEVLQADVKRGELYLKSTSMPVMGVAA
                     IVGLAGDVEGRVLFDMDKPTALKIASAMNGEDITSMDDLAKAM"
     gene            complement(295..1638)
                     /locus_tag="PLW80_06195"
     CDS             complement(295..1638)
                     /locus_tag="PLW80_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HPB66130.1"
                     /translation="MSAQAADTLEAGKRPDEKKERADKIDFKMVTFSLAGKEYGIDIM
                     NVKEIANAGRFTYVPNAAPFVRGVYNLRGDIISIIDLRTMFHLPAERKDDDSLESLLI
                     LRVEDHAFGVIVDNIDKVVGVSSAGIQPPHPIFGDINVKYIKGIIENQGKLYIILNVE
                     KLFAPKDEVPEQEMSALRRGPAPVAAPATMVAPGPAGDSGPVGDVNAEFIRETLFAFR
                     RFGVSAVNDGWFKARYTEWKGMRKPTDLQLREQADADAYLETFYSRCTGELWSEDYAA
                     AVVEALPKVESRSINVWNPGCGKGYETYSLAVALRRAYPEARIKIWANDSDLLAISMA
                     PNMVFTPENVPEHYLDHMVKGKNGYTFNQSIRESIFFEFHDVMNSNPVPPVDFILCRD
                     TVSFLSAADQGKLFGDFCEKAKQGGAVILGTNERLPLDGWRRVGEGPAAVYVRTA"
BASE COUNT          287 a          531 c          521 g          351 t
ORIGIN      
        1 catcgccttg gccaggtcgt ccatcgacgt tatatcctcg ccgttcatcg ccgaggcgat
       61 cttgagcgcg gtcggcttgt ccatgtcgaa caggacacgg ccttcgacgt ccccggcgag
      121 gccgacgatg gccgccacgc ccatgaccgg catcgaggtc gacttcaggt acagctcgcc
      181 tcgcttgacg tcggcctgca gcacttcctt catcacgttg aaggcgctct ccacgaaggg
      241 gttgatatac tctactctca tgccgttctc ccgtactgtc gttttagggc ccgactaggc
      301 cgtcctcacg tacaccgcgg cggggccttc cccgacgcgc ctccaaccgt ccaggggcag
      361 gcgctcgttc gtaccgagga tgacggcgcc tccctgcttc gctttctcgc agaaatcgcc
      421 gaataactta ccctggtcgg ccgcggacag gaaggatacg gtatcgcggc acaggatgaa
      481 gtccactggc ggaacgggat tcgagttcat cacgtcgtgg aactcgaaga atatggactc
      541 ccggatcgac tggttgaagg tatacccatt cttgcccttc accatgtggt cgaggtagtg
      601 ctccggcacg ttttccgggg taaagaccat gttcggggcc atcgagatgg ccaacaggtc
      661 ggaatcgttg gcccaaatct tgatacgggc ctcaggatag gcgcgcctga gggcgacggc
      721 cagggaatag gtctcatagc ccttgccgca gccggggttc cagacgttga tggacctgga
      781 ttccaccttg ggtagcgctt cgacgacggc ggcggcgtaa tcctccgacc acagctcgcc
      841 ggtacagcgc gagtagaagg tctccaggta cgcgtcggcg tccgcctgct ctcgtagctg
      901 gaggtcggtc ggcttgcgca tgcccttcca ctctgtgtag cgcgccttga accagccgtc
      961 gtttactgcc gacaccccga agcgcctgaa cgcgaacagg gtctcccgga tgaactccgc
     1021 gttgacgtcc ccgacggggc ccgagtcacc cgccggaccc ggagctacca tggtcgcagg
     1081 cgccgcgacg ggggccggac cgcggcgcag ggccgacatc tcctgctcgg gcacctcgtc
     1141 cttaggcgcg aagagcttct cgacgttcag gatgatgtac agcttgccct ggttctcgat
     1201 gatgcccttg atgtacttga cgttgatatc cccgaagatc gggtgaggcg gctggatgcc
     1261 ggcgctcgag acgccgacga ccttgtcgat gttgtcgacg atgacgccga aggcgtgatc
     1321 ctcgacacgc aggatgagga ggctctcgag cgagtcgtcg tccttacgct ccgccggcag
     1381 gtggaacatc gtacgtaggt cgatgatcga gatgatatcg ccccgcaggt tatagacgcc
     1441 gcggacgaag ggggctgcgt tcggcacgta ggtgaagcgc ccagcgttcg ctatctcctt
     1501 gacgttcatg atgtcgatgc cgtattcctt gccggcgagc gagaaggtga ccatcttgaa
     1561 gtcgatcttg tcggcccgtt ccttcttctc gtccggccgc ttcccggcct cgagcgtatc
     1621 ggcggcttgt gcgctcatgc gatccttact tccgtggtca acgtatggtc agctcgaggc
     1681 gctgtcgctc
//
LOCUS       DAOOLN010000433         1688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_92085_length_1688_cov_3.500919, whole genome shotgun sequence.
ACCESSION   DAOOLN010000433 DAOOLN010000000
VERSION     DAOOLN010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92085_length_1688_cov_3.500919"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /gene="selA"
                     /locus_tag="PLW80_06200"
     CDS             complement(<1..1073)
                     /gene="selA"
                     /locus_tag="PLW80_06200"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HPB66131.1"
                     /translation="MDISERASGIPQVERLLADDEIRAFFPLVSRPLAARTVSAALAA
                     ERSRALADPAYRADPTVCRASVLSALRALELKRLRPVMNGTGIVLNTNLGRSPVSAEA
                     WDDARGANVGYAAVEYDLAAGKRGSRGGLCGELAATLAGAEAALVVNNNAAGVLLALS
                     AIAKGRDVLVARGEQVQIGGGFRVPDVLELAGARFVEVGTTNIVDTDDYRRAVGSDTA
                     CALIVHTANFALRGFCSKPSPAEIVTAMPDGLPVIVDQGSGCYFDGLPGEARVRSYID
                     AGCALVCFSGDKLLGGPQAGIIAGRADLIAKLARHPLYRAFRPGKTVLSLLERVLVER
                     LNGEGGPAQAAVSYPTDELKKLAR"
     gene            complement(1075..1163)
                     /locus_tag="PLW80_06205"
     tRNA            complement(1075..1163)
                     /locus_tag="PLW80_06205"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1127..1129),aa:Sec,seq:tca)
     gene            1332..>1688
                     /gene="selD"
                     /locus_tag="PLW80_06210"
     CDS             1332..>1688
                     /gene="selD"
                     /locus_tag="PLW80_06210"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1383..1385,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HPB66132.1"
                     /translation="MMDKDGIRLTSLCSAAGUAAKMGPEALAQVLRPLAGLVPEQRAS
                     SLISGLDEGDDAAVYRIDDDTCIIFTADFFTPIVDDPYDFGAIAAANAMSDVYATGGE
                     PILALNLAAFPPDLPPS"
BASE COUNT          270 a          544 c          593 g          281 t
ORIGIN      
        1 cgggccagct tcttgagttc gtcggtcgga taggataccg ccgcttgagc cgggccgccc
       61 tcgccgttga ggcgctccac gaggacgcgc tcgagcaacg acaggacggt cttgcccggc
      121 ctgaaggcgc ggtacagcgg atgcctggcg agcttcgcta tgaggtcggc gcggccggcg
      181 atgatgccgg cctgggggcc gccgagcagt ttgtctccgg agaagcagac cagggcgcag
      241 cctgcatcga tgtaggagcg gacgcgcgcc tcgcccggaa ggccgtcgaa gtagcagccg
      301 gagccctggt cgacgatgac cggcaggccg tccggcatcg ctgtcacgat ctcggcgggg
      361 cttggcttcg agcagaagcc tcggagtgcg aagttggccg tgtgcacgat cagggcgcag
      421 gcggtatccg agccgacggc gcgacgatag tcgtccgtat cgacgatgtt cgtggtgcct
      481 acctcgacga agcgggcgcc agcgagctcg agcacgtccg gcacgcggaa gccgccgcct
      541 atctgtacct gctcgcccct ggctactagt acgtcgcggc ccttggcgat ggcgctcagg
      601 gcgaggagga cgcccgcggc gttgttgttg acgacgagcg cggcttcggc tccggccaac
      661 gtcgcggcca gctcgccgca gaggccgccg cgactaccgc gctttccggc cgccaggtcg
      721 tattcgacgg cggcgtagcc gacgttggcg ccgcgcgcgt cgtcccaggc ctcggccgac
      781 acgggggacc ggccgaggtt cgtgttgagc acgatgcccg taccgttcat gacgggtcgc
      841 aagcgcttga gctcgagcgc cctgagcgcc gataggaccg aggcccggca taccgtcggg
      901 tcggctcggt aggccgggtc ggcgagcgcg cgagagcgct cggcggccag cgcggccgat
      961 accgtgcgcg cggctagggg tcgcgatacg agcgggaaga acgcccgtat ctcgtcgtcg
     1021 gccaggaggc gctcgacctg tggaatgccc gatgcgcgtt cgcttatgtc catacggaag
     1081 aggcgggaat cgaacccacc accgcgcgag gcgcggccaa cggttttgaa gaccgcggga
     1141 gccaccagac cccatcagct tccctgtcgt ctgcatacta cgggacgccc acggcgttgt
     1201 caacgcgagt gtatacgtac gttagacgtt ggatccggcg cttgcgcttg ccaagccgcc
     1261 gaatcgctga taggcttgct cgcatgtcgc ggtatatacc catcgagtgg gtaattcgcc
     1321 ggaaaggagg gatgatggat aaggacggca tcaggctgac gtcgttgtgc tccgctgccg
     1381 ggtgagcggc taaaatgggc cccgaggccc tggcgcaggt tctgcgtcct ttagccggtc
     1441 tcgtgccgga acagcgcgcg tcatccctta tttccgggct ggacgagggc gacgacgccg
     1501 cggtgtaccg gatcgacgac gacacatgca ttattttcac ggccgatttc ttcacgccga
     1561 tagtggacga tccgtacgat ttcggcgcga tagcggcggc caacgccatg tccgacgtgt
     1621 acgctaccgg aggcgagccc atcctagccc tcaacctggc ggcgttcccg cctgacctgc
     1681 cgccctcg
//
LOCUS       DAOOLN010000434         1686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_92272_length_1686_cov_3.086450, whole genome shotgun sequence.
ACCESSION   DAOOLN010000434 DAOOLN010000000
VERSION     DAOOLN010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92272_length_1686_cov_3.086450"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1686)
                     /locus_tag="PLW80_06215"
     CDS             complement(<1..>1686)
                     /locus_tag="PLW80_06215"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPB66133.1"
                     /translation="YPDLLLGLAVDTRLGSNGGFGWGLAADLGVIGLAGDVGALKNLV
                     WGASLRNMGKAFEAPGAVGLSGGLASAYDAPFTPQVGASADLIRADKAGLKLSANADL
                     WLPTFQNAVFATGLRLGWRDRAFLRLGWDFNLREAIAGVDQGLLPSFGLGASFAIDRE
                     SDDSLLSKAGLDRSEIRPSFSAAELYGGVWAFSGGANVPVGVLDRVPPRITVSYPAVE
                     HDAYYISPNSDGARDLLTLPLSITDQRYVQSFSLKVLDESGAVVKTMANKESRPENQD
                     LKGLLGRLLYVKKGVDVPAELEWNGLTDSGAAAPDGRYFVVIEAVDDNGNVASTERWP
                     VVIDTVPPSVKASGPAGDALIFSPDGDGSKDEFAIAQEGSVEDSWVAVVTDAAGATVR
                     SWTTASAAPSALRWDGKNDSGKVVPDGVYSYRVAAVDRAGNQGSARIDNIIVNTQQPP
                     VGVSIDLAAFSPDGDGVKDSLTLTPNVPVKAGLASWRLAVLDDKGVERWAASGVGADS
                     IEPAYRFSGKDASGAALPEGTYRTRLSVSYVNGHGPVAHSPNFVIDVTPPAASV"
BASE COUNT          270 a          559 c          608 g          249 t
ORIGIN      
        1 gacggaggcc gccggcggcg tgacgtctat gacgaaattc ggagaatgcg ccacggggcc
       61 gtggccgttc acataggaga ccgacaaccg ggtccggtag gtcccctcgg gcagggccgc
      121 cccggaggcg tccttacccg agaaacggta ggccggctcg atcgaatcgg cgccgacgcc
      181 agaggcggcc cagcgctcga cgcccttgtc gtcgaggacg gcgaggcgcc acgacgcgag
      241 gccggccttg accggcacgt tcggcgtcag cgtcagagag tccttgacgc cgtcgccgtc
      301 ggggctgaag gcggccaggt cgatggacac gccgaccggc ggctgctggg tattgacgat
      361 gatgttgtct atcctggcgg agccctggtt accggcccgg tcgacggcgg cgacacggta
      421 ggagtacacg ccatcaggca ccaccttgcc ggagtcgttc ttgccgtccc agcgcagggc
      481 cgacggggcg gccgacgcgg tcgtccagga gcgtaccgtc gctccggcgg cgtcggtgac
      541 gacggcaacc caggagtcct cgacggaccc ttcctgcgct atggcgaact cgtccttgct
      601 gccatcgccg tccgggctga aaatcaaggc gtccccggcg ggacccgagg ccttgacgct
      661 cggcgggaca gtatcgatga cgaccggcca gcgctcggtg gaggccacgt tgccgttatc
      721 gtcgaccgcc tcgatgacga cgaagtagcg cccatccggg gccgcggcgc cggagtcggt
      781 taggccgttc cactcgagct cggccgggac gtcgactccc ttcttcacgt agagcaggcg
      841 tcccaggagg cccttcaggt cctggttctc gggcctggac tccttattgg ccatggtctt
      901 gaccacggcg cccgactcgt cgagtacctt gagcgagaag ctctggacgt agcgctggtc
      961 ggtgatcgag agcggcagcg tcagaaggtc gcgagcgccg tcgctgttgg ggctgatgta
     1021 gtaggcgtca tgctcgaccg cgggatagct cacggtgatc ctgggcggga cccgatcgag
     1081 tacgccgacc ggcacgttcg ctccgcccga gaaggcccat acgccgccgt acagctcggc
     1141 ggccgagaag gatgggcgaa tctccgaacg gtccaggccg gccttcgaga ggagcgaatc
     1201 gtcgctctcg cggtcgatgg cgaagctcgc gccgaggcca aagctgggta gcaggccctg
     1261 gtcgacgccg gcgatcgcct cgcgcaggtt gaagtcccag ccgagcctga ggaaggcgcg
     1321 gtcgcgccag ccgagcctca ggcccgtcgc gaagacggcg ttctggaagg tgggcaacca
     1381 taggtcggcg ttggccgaca gcttgaggcc ggccttgtcg gcccgtatga ggtcggcgga
     1441 ggcgccgacc tgcggggtga acggagcgtc ataggccgag gcgagcccgc ccgacagccc
     1501 caccgcgccg ggagcctcga acgccttgcc catgttgcgc agcgaggcgc cccagacgag
     1561 gttcttgagc gcgccgacgt cgccggccag gcctatcacg ccgaggtcgg cggcgaggcc
     1621 ccagccgaaa cctccgttcg agccgagccg cgtatcgacg gccagtccga gcagcaggtc
     1681 cgggta
//
LOCUS       DAOOLN010000435         1686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_92283_length_1686_cov_2.822195, whole genome shotgun sequence.
ACCESSION   DAOOLN010000435 DAOOLN010000000
VERSION     DAOOLN010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92283_length_1686_cov_2.822195"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PLW80_06220"
     CDS             <1..918
                     /locus_tag="PLW80_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HPB66134.1"
                     /translation="ENGLVHGRGTSDQTGGAAAMITAGRILKELKYAGDYSVWFVFSI
                     IEEDCDGMCWKYLIEEEKFVPDFFVSTEPTSLRLYRGHRGRMEMEVHLKGVSCHGSAP
                     ERGVSAAYKAARAALAIEALNAALKPDDDNFLGKGTVTVSQMKVQGPSQCAVPDEAML
                     YLDRRLTWGETKDLAIQQVKDAVLAAGVDAVEVTMPEYAIPSWTGKDYRQELYFPTWK
                     FPADHPFVKAGVAAHEAVFTAAPVVDKWTFSTNAVAVAGRHGIPSIGFGPGDENQAHA
                     PNEITRVDDLWKAAAFYAALPYALEDAKL"
     gene            1127..>1686
                     /locus_tag="PLW80_06225"
     CDS             1127..>1686
                     /locus_tag="PLW80_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPB66135.1"
                     /translation="MRWFYRCPGCGAEYDIEPGRYLCDACAARQEPDRPLEGVLECAW
                     EGDYSGGPDDIPLPVAKEHLPPLPVGGTPLWEPRRLRAETGYPRLFLKDDTCEPTGSF
                     KDRASYLVAGFAKERGISEIALASTGNAASSMSGIGAAAGLKITVFLPKKAPVAKRVQ
                     VLQYGAELHEVDGTYDVAFDRSLAYS"
BASE COUNT          297 a          569 c          553 g          267 t
ORIGIN      
        1 gagaacggcc tcgtccacgg ccgcggcacc tcggaccaga ccggcggcgc ggccgcgatg
       61 atcaccgccg gccgcatcct caaggagctc aagtacgcgg gcgactattc cgtctggttc
      121 gtcttctcca tcatcgagga agactgcgac ggcatgtgct ggaagtacct gatcgaggag
      181 gagaagttcg tgcctgactt cttcgtctcc acggagccta ccagcctccg cctgtatcgc
      241 ggccaccgcg gccgcatgga gatggaggtg cacctgaagg gtgtctcctg ccacggttcg
      301 gcccccgaac gcggcgtctc ggccgcctat aaggccgccc gcgccgccct cgccatcgag
      361 gcgctcaacg cggccctcaa gccggacgac gacaacttcc tgggcaaggg cacggtgacc
      421 gtgtcgcaga tgaaggtgca gggcccgtcg cagtgcgccg tgccggacga ggccatgctg
      481 tacctggacc gccgcctgac ctggggcgag accaaggacc tggccatcca gcaggtcaag
      541 gacgccgtcc tggccgccgg cgtcgacgcc gtcgaggtga ccatgcccga gtacgccatc
      601 ccgagctgga ccggcaagga ctaccgccag gagctgtact tcccgacctg gaagttcccg
      661 gccgatcacc ccttcgtcaa ggccggcgtc gccgcccacg aggccgtctt cacggccgcg
      721 ccggtcgtcg ataagtggac cttctcgacc aacgcggtag cggtggccgg ccgccacggt
      781 atcccgagca tcggcttcgg ccccggcgac gagaaccagg cccacgcccc caacgagatc
      841 acgcgcgtgg acgacctctg gaaggccgcc gccttctacg ccgccctccc ctacgcgctg
      901 gaagacgcca agctgtagga ttaggctgta ctaggaaagc cggcgaagag aataagagca
      961 gaagagggaa ggaagagccg atgttgatgg atggatcaga gaagcaagga tcgaagacgc
     1021 gctcatggtc gccctcaata tgccattttc ttctcttccc tcttgaatcg ctcttattcc
     1081 cttatcctct ttgttcaaat cagggtttat ggctaaggag tatacggtga ggtggttcta
     1141 tagatgcccc gggtgcgggg cggagtacga tatcgagccg gggcgctacc tctgcgacgc
     1201 gtgcgccgcg aggcaggagc cggaccggcc gctcgagggc gtgctcgagt gcgcctggga
     1261 gggcgactac tctggcggtc ccgacgacat cccgcttccg gtggccaagg agcacctgcc
     1321 gccgctgccg gtcggaggca ccccgctctg ggagccgcgc cgcctgcgcg cggagaccgg
     1381 ctacccgcgg ctgttcctca aggacgatac ctgcgagccg accggctcgt tcaaggaccg
     1441 agcctcgtac ctggtggccg gcttcgccaa ggagcgcggg atatcggaga tagcgctcgc
     1501 gagcaccggc aacgccgcct cgagcatgtc ggggatcggc gccgctgccg gcctcaagat
     1561 cacggtgttc ctcccgaaga aggccccggt cgccaagcgc gtccaggtgc tgcagtacgg
     1621 ggcggagctc cacgaggtcg acgggaccta cgacgtggcg ttcgaccggt cgctcgcgta
     1681 ctcgaa
//
LOCUS       DAOOLN010000436         1686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_92288_length_1686_cov_2.469037, whole genome shotgun sequence.
ACCESSION   DAOOLN010000436 DAOOLN010000000
VERSION     DAOOLN010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92288_length_1686_cov_2.469037"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1474)
                     /locus_tag="PLW80_06230"
     CDS             complement(<1..1474)
                     /locus_tag="PLW80_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPB66136.1"
                     /translation="MSSTRCRPYIVALFALAALAPAMAQAPATVAMSDFVVNSDNPAF
                     TYLGKGFAEIIAFEVRKAPQVRLVDREQRNKQLEEMEFSLSGLTDPSAQLEIGKLLSV
                     RFLVSGSITDMGGPLLVSLSMLDVQTGAIVWNDQLTATSGKYAYIGAYFGKSLLRHFN
                     ATVSKETERALVASKDGDEASVIALSRGIEALDKGDTDEAKKQLTAARSIDPDNQVAA
                     ALLSKLASASAKFKVVPERSVSYYNPAYLGGMEKDKAYFNLSFANLAWGLPNEDKGES
                     QDYWLALDESGDYGTSERQMNLVAGYGFPLNGVLGLCAEVSGGGELSNAILRQYAPGD
                     WMASGNDYTYYISGGSITAGLRVSPWLSLGLGATVSYKKRDYYSEYLGSPPPELADPM
                     NGYWLDTGISFGGMLAAALRSRSGGLSWDVSASWSNEVLHYFDTDPAALEFYEYGAPL
                     YVEQTLSWSPAGQKTFLALKQVNDLYFDRELYYSRLMPCVE"
BASE COUNT          317 a          526 c          528 g          315 t
ORIGIN      
        1 gctcgacgca cggcatgagg cggctatagt agagttcccg gtcgaagtac aggtcgttga
       61 cctgcttcaa ggcgaggaag gtcttctggc cggccggtga ccaggagagg gtctgttcca
      121 cgtacagcgg cgctccgtac tcgtaaaatt cgagcgccgc gggatcggta tcgaagtagt
      181 gcagcacctc gttcgaccag gacgcggaga catcccatga aagcccgccc gaacgggacc
      241 taagcgcggc cgcgagcatc ccgccgaacg agatccccgt atccaaccaa tatccattca
      301 tcggatccgc tagttccgga gggggcgatc cgagatactc gctatagtag tcgcgcttct
      361 tatacgatac ggtagcgccg agtccgaggc tcagccatgg cgagactcgt agcccagcgg
      421 tgatcgagcc tccggatatg tagtaggtat agtcgttacc cgaggccatc caatcgcccg
      481 gagcgtattg cctaagtatc gcgttgctca gttcaccccc gcccgagacc tctgcgcaca
      541 ggccgagaac gccgttgagg gggaagccgt atccggcgac caggttcatc tggcgctcag
      601 aggttccata gtctcccgac tcgtccaggg ccagccaata atcctggctc tctcccttgt
      661 cctcgttcgg caagccccag gccaggttgg cgaaggacag gttgaagtac gccttatcct
      721 tttccatgcc tccgagataa gccgggttgt agtagctgac ggagcgctca ggtacgacct
      781 tgaacttggc cgacgccgag gctagcttcg atagcagggc agccgcgacc tggttgtccg
      841 gatcgatact ccgcgcggcg gtcagttgct tcttcgcctc gtccgtatcg cccttgtcca
      901 acgcctcgat gcccctggac agggcgatca ccgacgcctc gtcgccgtcc ttggacgcga
      961 ccagggcccg ctctgtttcc ttggagaccg tcgcattgaa atggcggagc agcgacttgc
     1021 cgaagtaggc gccgatgtac gcgtacttgc cgctcgtggc cgtcagttgg tcgttccaca
     1081 cgatcgcgcc ggtctggacg tcgagcatcg aaaggctgac gagtagcggc ccgcccatgt
     1141 cggttatcga tccggacacc aggaaacgta ccgaaaggag cttgccgatc tcgagctggg
     1201 ccgaggggtc cgtcaggccg gacagggaga attccatctc ctcgagctgc ttgttccgtt
     1261 gctcccggtc gacgagccgt acttgcgggg ccttcctcac ctcgaaggcg atgatctcgg
     1321 cgaagccctt gccgaggtac gtgaacgcgg gattatccga gtttacgacg aaatcgctca
     1381 tggcgacggt cgctggagcc tgagccatcg ctggcgccag ggcggcgagg gcgaatagcg
     1441 cgacgatgta cggacggcac ctcgtactgc tcatatgcga cctcctcggt acgatatgcg
     1501 cataagcata tgcaccgtgc gcgtcaaggc aagcccccgc cgccgcgcgc cggaactagg
     1561 gtatcgatcg gagctcggca cggcggccga tggagtgcgc gatggcctcg acccgctcgt
     1621 agacctcgag gcgatggtcc aggaagtcgg tcaggacgac gaggcggccg tccggcgaca
     1681 cggcca
//
LOCUS       DAOOLN010000437         1686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_92302_length_1686_cov_2.053342, whole genome shotgun sequence.
ACCESSION   DAOOLN010000437 DAOOLN010000000
VERSION     DAOOLN010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92302_length_1686_cov_2.053342"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PLW80_06235"
     CDS             complement(<1..603)
                     /locus_tag="PLW80_06235"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPB66137.1"
                     /translation="MVVVDGVRLNDARTGYADLSGVSLAGVERVEVLRGGASALYGAD
                     AIAGVVYVTTARARSDSFSATVSTTAYPLAAAEGASALLASQELSFAARKAVGAATVS
                     VSGGAERAADELPVADEESYSVRGNTGLLAGRAGLGVVSPLLGGTVTASASGSVSDKG
                     VPGSVSYASPNAYQKEYAARGIIGWGTDALADGNASVDADL"
     gene            complement(576..899)
                     /locus_tag="PLW80_06240"
     CDS             complement(576..899)
                     /locus_tag="PLW80_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66138.1"
                     /translation="MRRIACIVAIAAVASAGVAAQGAVETVDLGQAVVVGAWAPSELS
                     PTAATVVIDAEEIAASGASTLDELLDGRAGLEVREYGYPARPDRCRYAAVRAAMSWWW
                     WTAFA"
     regulatory      complement(1091..1241)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1301..>1686)
                     /locus_tag="PLW80_06245"
     CDS             complement(1301..>1686)
                     /locus_tag="PLW80_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66139.1"
                     /translation="YFIPLVVSGSIADGDIVSIEAIDIERSEGYEPARIWLRGDLLSY
                     WDDYDLGAKANRRIEALVRANLPLGSQLGQDRSSVGDVIIAVIDYTGGKPEAWKAVLS
                     INGELKEFGGQIETDAPFLVAPRMF"
BASE COUNT          255 a          611 c          539 g          281 t
ORIGIN      
        1 caggtcggcg tcgacgctcg cgttcccgtc ggccagggcg tccgtgcccc atcctatgat
       61 acccctagcc gcgtactcct tctggtaggc gttcggggac gcgtacgaga cgctgccggg
      121 cacgcctttg tcggagacgg agccgctcgc ggacgccgtt accgttccgc cgaggagcgg
      181 cgagaccacg ccgaggccgg cgcgtccggc caggagaccg gtattgccgc gtactgagta
      241 gctttcttcg tcggccaccg gtagctcgtc ggcggccctt tccgcgccgc ccgagacgct
      301 gacggtcgcg gccccgacgg ctttccgcgc ggcgaagctc agctcctggc tcgccagcag
      361 cgcgctcgcg ccctcggccg ccgcgagagg atacgccgtc gtcgagacgg tggccgagaa
      421 cgaatcgctt ctggccctcg ccgtcgtcac gtagaccacg ccggcgatcg cgtccgcccc
      481 atacagcgcc gacgctcctc cgcgcagcac ctcgacgcgc tcgacgccgg ccagcgatac
      541 gcccgagagg tcggcgtagc cggtacgcgc gtcgttcagg cgaacgccgt ccaccaccac
      601 cacgacatgg ccgcccgtac cgccgcgtac cgacagcgat ccggccgagc cgggtacccg
      661 tactcgcgca cctcgagccc ggctcgcccg tcgagcagct cgtccagagt gctcgcgccc
      721 gaggcggcta tctcctcggc gtcgatgacc accgtcgccg cggtcggcga cagctccgac
      781 ggggcccagg cgccgacgac gacggcctgg cccaggtcga cggtttcgac cgccccctgg
      841 gccgcgacgc cggccgaggc tacggcggcg atcgctacga tgcatgcgat cctgcgcatg
      901 atgcgaatcc tttccgtccg gtcacgccgg acgagagagg acgatgagcc tgtaaatcag
      961 cggaatcgtc gcggaccggt cagtcgcagc ggcgacgccg gcccgccggg aaggcgggtt
     1021 tcatgcaggg actcctcgat cctcgaaggc ggccccgccg cctatcggcg gagcctttca
     1081 gtcccggtcg gtatcctggc tcgcgcgtcg aaccggtacc cggacgcctt ctcatcggcc
     1141 ttcaggccga caatggccgc ggccgagcct ggtagctctg ccgcatgtcc gttccgtagc
     1201 gcttacagtg gcgggtccgc cgcggattct caccgcgttc cacgttcccg ggactggtcg
     1261 cccgctagca tgccctgggc atgcgcgttg gtcaagtggg ctagaacatc ctcggcgcta
     1321 ccaggaacgg agcgtcggtc tcgatctgcc cgccgaattc cttaagctcg ccgttgatcg
     1381 ataggacggc cttccaggcc tcgggcttgc cgcccgtata gtctattacc gcgatgatga
     1441 cgtcgccgac ggacgagcga tcctgcccta actgactgcc tagcgggagg ttcgcgcgaa
     1501 cgagggcctc tatacgacga ttcgccttgg ctccaaggtc gtaatcgtcc cagtatgaga
     1561 gcaagtcgcc ccgtagccag atcctggccg gctcatagcc ttccgaccgt tcgatatcga
     1621 tcgcttcgat agagactatg tctccgtcgg ctatcgatcc gctcacgacg agcgggatga
     1681 aatagt
//
LOCUS       DAOOLN010000438         1681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_92732_length_1681_cov_2.476630, whole genome shotgun sequence.
ACCESSION   DAOOLN010000438 DAOOLN010000000
VERSION     DAOOLN010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92732_length_1681_cov_2.476630"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PLW80_06250"
     CDS             complement(<1..429)
                     /locus_tag="PLW80_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPB66140.1"
                     /translation="MTVGNRMSRRLVTVGADTAVPEAEALMRREKIHRLPVVGKKGEL
                     LGIVTASDLRRASAPPATSLDIYELHYLISRLKVESVMTKDPVTVSEDLPVEEAARIM
                     DDNRIAGLPVTRGGELVGIITESDLFRLFIDLFGARVEDIV"
     gene            complement(446..1153)
                     /locus_tag="PLW80_06255"
     CDS             complement(446..1153)
                     /locus_tag="PLW80_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB66141.1"
                     /translation="MMELRDVCTYYGNIQALRGISLSVEEGEIVTLIGANGAGKTTTL
                     MSICGIAQIRSGQVLLDGEDISRKSPHSIVAMGLSQVPEGRRIFPQLTVLENLDMGAF
                     LRRDKATVRVDLEEVFDLFPRLAERRNQQGGTLSGGEQQMLAISRALMARPRILLLDE
                     PSLGLAPLIVQNIFDIIKRINEERRTTVFLVEQNANMALKIADKGYVLQNGSIKMADS
                     AERLLANEEVRKAYLGL"
     gene            complement(1161..>1681)
                     /locus_tag="PLW80_06260"
     CDS             complement(1161..>1681)
                     /locus_tag="PLW80_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB66142.1"
                     /translation="ARTFQNIRLFGNMTVLENVMIGRHNALAAGVIRAIARDAATRDE
                     EARLIDDCRRILVKMKLDPFINETARNLPYGAQRRLEIARALATDPFLLLLDEPVAGM
                     NPHETKELEETIDLIRDEEKVTILLIEHDMSLVMNVSERIYVLDYGRLIAEGTPEEIK
                     GNPEVIRAYLGE"
BASE COUNT          266 a          557 c          524 g          334 t
ORIGIN      
        1 gacgatgtcc tcgacgcgcg cgccgaagag gtcgatgaac agcctgaata ggtcggactc
       61 cgtgatgatg cccacgagct cgccgccgcg cgtcaccggc aggccggcga tgcggttgtc
      121 gtccatgatc ctcgcggcct cctcgaccgg caggtcctcg gacacggtga ccggatcctt
      181 cgtcatcacc gactccacct tgagcctgga gatcaggtag tggagctcgt agatgtcgag
      241 cgaggtggcc ggcggcgccg aggcgcgtcg caggtccgag gccgtgacga tgccgagtag
      301 ctcgcccttc ttgccgacca ccggcaggcg gtggatcttc tcgcggcgca tcagggcttc
      361 cgcctccggt acggccgtat cggcgcctac ggtaaccagg cgccggctca tgcgattgcc
      421 tacggtcatg ttgcctcctc gctgatcata gcccgaggta cgccttgcgt acctcttcgt
      481 tagccaacag acgctcggcc gagtcggcca tcttgatcga gccgttctgc agcacgtagc
      541 ccttgtcggc tatcttgagc gccatgttgg cgttctgctc gacgaggaac acggtggtcc
      601 tgcgttcctc gttgatgcgc ttgataatat cgaaaatatt ctggacgatc agcggagcca
      661 ggcccagcga cggctcgtcg agcagcagga tccgcggcct ggccatcagg gcccgagata
      721 tcgccagcat ctgctgctcc ccgccggaca gtgtcccgcc ctgctggttc cgccgctccg
      781 cgagcctcgg gaacaggtcg aagacctcct ccaggtcgac gcggaccgtg gccttgtcgc
      841 gacgcaggaa ggcgcccatg tccaggttct ccagcacggt cagctgaggg aagatgcgcc
      901 ggccctccgg cacctgggac aggcccatcg cgacgatgga gtgagggctc ttcctcgaga
      961 tgtcctcgcc gtcgagcagc acctggccgg agcgtatctg ggcgatgccg cagatcgaca
     1021 tcagcgtggt ggtcttaccg gccccgttgg cgccgatcag cgtgacgatc tcgccctcct
     1081 ccaccgacag cgagatgccg cgcagagcct ggatattgcc atagtacgtg cagacgtccc
     1141 tgagttccat caaagccatg tcattctcct aaatacgccc tgatgacctc gggattcccc
     1201 ttgatctcct ccggcgtgcc ctcggctatc agccgaccgt agtcgaggac gtagatgcgc
     1261 tccgatacgt tcatcacgag gctcatgtcg tgctcgatga gcagtatcgt caccttctcc
     1321 tcgtcgcgta tcaggtcgat cgtctcctcg agctccttcg tctcgtgcgg gttcatgccc
     1381 gcgaccggtt cgtcgagcag caagaggaag gggtcggtcg ccagggccct ggctatctcc
     1441 agccgccgct gggcgccgta cggcaggttc ctggccgtct cgttgatgaa cgggtcgagc
     1501 ttcatcttga cgaggatgcg gcggcagtcg tctatcagcc gcgcctcctc gtcgcgcgtc
     1561 gcggcgtccc tggctatggc ccggatgacg ccggcggcga gcgcgttgtg ccggcctatc
     1621 atcacgttct cgagcacggt catattcccg aatagcctga tgttctggaa ggtgcgcgcg
     1681 a
//
LOCUS       DAOOLN010000439         1681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_92740_length_1681_cov_2.319803, whole genome shotgun sequence.
ACCESSION   DAOOLN010000439 DAOOLN010000000
VERSION     DAOOLN010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92740_length_1681_cov_2.319803"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1681)
                     /locus_tag="PLW80_06265"
     CDS             complement(<1..>1681)
                     /locus_tag="PLW80_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010957181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB66143.1"
                     /translation="FSPIRVNRLTIKNRLVMAPMGNVSMADETGRPSAKMIEYFTARA
                     RGGVGLITSGLVPVSHGVDPTVTELGDLSYFPRIDRSRSVFAGWRDLAERCHAHGARF
                     FIQLTPGLGRVGSPECLINKKKLPVSASWNPNFYMPALPCRPLTDGQARRIIKNSGQA
                     AADAKAATIDGVYLHGHEGYLLEQMTNPAFNRRKLGAFADWQAFGVKMIEEIRRRVGP
                     DYPIMYRIDLSLALEETYGERMDAVPSLKKFKGGRTPEQTLEYMESLVKAGVDLFDVD
                     LGCYDNWWLPHPPSSMAPGCYLEVSRIVKERFAERKLLSNAGLPVPVVAVGKLGYPDL
                     SEKALRDGDCDMVMLGRPLLADPEWPNKAFAGRVADIAPCIGDQEGCINEFVEGGHPQ
                     CAVNARTGFEDILDGSELAPAAVFKKIAVVGGGPGGMQCAVIAARRGHDVTLYESRVE
                     LGGMLVPGSRPAIKFDIVNYLEKLRHDVKTAGKDYKLKVKLGEAPSAAELKRGKYDAI
                     VTATGSVQARPPVPGIDSANVRLAVDVLRDPSLASGAQKIVVVGGGVVGLRD"
BASE COUNT          257 a          519 c          623 g          282 t
ORIGIN      
        1 cgtcgcgcag tccgacgaca ccaccgccga cgacgactat cttctgggct ccggaggcca
       61 gggacgggtc gcggaggacg tcgaccgcca gcctgacgtt cgcggagtcg atgcccggta
      121 cgggcggcct ggcctggacg gaaccggtgg cggtgacgat ggcgtcgtac ttgcccctct
      181 tgagctcggc cgcgctcggc gcctcgccaa gcttcacctt gagcttgtag tccttgccgg
      241 cggtcttgac gtcgtgccgc agcttctcga gataattgac gatgtcgaac ttgatcgcgg
      301 ggcgcgagcc ggggacgagc atgccgccga gctcgacgcg ggactcgtac agggtgacgt
      361 cgtggccgcg gcgggcggct atgacggcgc actgcatgcc gccggggccg ccgccgacga
      421 cggctatctt cttgaaaacg gcggccgggg cgagctcgga gccgtcgagg atatcctcga
      481 aaccggtgcg cgcgttgacg gcgcactggg gatggccgcc ctcgacgaac tcgttgatgc
      541 agccttcctg gtcgcctatg cacggcgcga tgtcggcgac gcggccagcg aaggccttgt
      601 tgggccactc cgggtcggcg aggagggggc ggccgagcat gaccatgtcg cagtcgccgt
      661 cgcgcagggc cttttccgaa agatccgggt agcccagctt gccgaccgcg acgaccggca
      721 cgggcaggcc ggcgttggac agcagcttcc tctcggcgaa gcgctccttg acgatacgtg
      781 atacctcgag gtagcaaccg ggggccatcg acgaaggcgg gtggggtagc caccagttat
      841 cgtagcagcc gaggtcgaca tcgaagaggt ccacgcccgc cttcacgagg gactccatgt
      901 actcgagggt ctgttccggc gtgcggccgc ccttgaactt cttgaggctc gggacggcgt
      961 ccatgcgctc gccataggtc tcctcgagcg cgagcgatag gtcgatgcga tacatgatgg
     1021 ggtagtcggg gccgacgcgg cggcggatct cctcgatcat cttgacgccg aaggcctgcc
     1081 agtcggcgaa ggcaccgagc ttcctgcggt tgaaggccgg gttggtcatc tgctcgagga
     1141 ggtagccctc gtggccgtgg aggtagacgc cgtcgatggt cgcggccttg gcgtccgctg
     1201 cggcctggcc cgagttcttg atgatacgcc tggcctggcc gtcggtcagg gggcggcagg
     1261 gtagcgcggg catgtagaag ttggggttcc aggacgccga taccggcagc ttcttcttat
     1321 tgatcaggca ctcgggcgag ccgacgcggc cgaggccggg cgtgagctgg atgaagaagc
     1381 gggcgccgtg ggcgtggcat cgttcggcca ggtcgcgcca accggcgaac acggagcggc
     1441 tccggtcgat gcgcgggaag tacgacaggt cgccgagctc ggtaaccgtc gggtcgacgc
     1501 cgtggctgac gggcacgagg cccgaggtga tgaggccgac gccgccgcgc gcgcgagccg
     1561 tgaagtactc gatcatcttg gcgctcggcc gaccggtctc gtcggccatg ctgacgttgc
     1621 ccatcggggc catcacgagg cggttcttga tcgtcaggcg gttcacgcgg atgggcgaga
     1681 a
//
LOCUS       DAOOLN010000440         1679 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_92914_length_1679_cov_2.401478, whole genome shotgun sequence.
ACCESSION   DAOOLN010000440 DAOOLN010000000
VERSION     DAOOLN010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92914_length_1679_cov_2.401478"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="PLW80_06270"
     CDS             complement(<1..926)
                     /locus_tag="PLW80_06270"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HPB66144.1"
                     /translation="MTAGTRLAAMAMAVAVSAAACDQRPIVTLRYAHMSPLDSVAGMQ
                     AEYFARRARELSEGRLVVEVFPASVLGDTAEQLAMVRDGVIDIHHTTAGALASLVEGF
                     TVLDAPYRVSDPERLSRIVDLGSPLMTRLGDELRHGAGLRVLYTFYFGTRHLSFNGPV
                     LSPFDLRGAPIRANPYPIYELAVATLGGVPTPVDWNRIQAALATGAARGQENPIETIY
                     SSELQRWQSHLMLTGHIVGAEIVVMNERSWSLLPRREREALAEAAAEAGRWASAETSA
                     MEAARLEALKSLGMRVIGEADGLRLDLFKARA"
     gene            complement(923..>1679)
                     /locus_tag="PLW80_06275"
     CDS             complement(923..>1679)
                     /locus_tag="PLW80_06275"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPB66145.1"
                     /translation="GEIRDVILYVSPLELGDEELLHALVFDVTDQRIAEERSVAALEE
                     KTLLLREVYHRVKNNLQVISSLLSMQAAVTADRSVSTALQGAQDRVFAMSLAHEIVYQ
                     MPDISSVDAGEYASHLVAYLADEYKVPRDSVLAAYEPMPLSLERAVPFGLLLGEIVSN
                     ALKYGIPPGTDGRVRVSLSLDEAGRDRMVCLVVEDDGPGLPPEVADKGSLGISLIAGL
                     ADQLRGTVAWERPSGGGTRVTVRFDSSADGKAR"
BASE COUNT          255 a          572 c          583 g          269 t
ORIGIN      
        1 tcggctctgg ccttgaacag gtccagccgc agcccgtcgg cctcgccgat gacccgcatt
       61 cccaggctct tgagcgcctc gagcctggcg gcctccatcg ccgacgtctc ggcggaggcc
      121 cagcgaccgg cttccgcggc cgcttccgcc aaggcctcgc gctctcgcct cggcaatagc
      181 gaccaggagc gttcgttcat cacgacgatc tcggcgccga cgatatggcc ggtcagcatc
      241 aggtgcgact gccatctctg gagttccgac gagtagatcg tctcgatggg gttctcctgg
      301 ccccgcgccg cgccggtagc aagggcggcc tggatgcggt tccagtccac cggggtgggg
      361 acgccgccca gggtcgcgac cgcgagttcg tagatgggat agggattagc ccgtatcggg
      421 gcgcctcgca ggtcgaacgg cgacagcacc gggccgttga agctcaggtg gcgcgtaccg
      481 aaatagaacg tgtacagcac cctgaggccg gccccgtgcc ggagctcgtc gccgagcctc
      541 gtcatcaggg gcgagccgag gtcgacgata cgggagagcc gctccgggtc ggagacccgg
      601 tagggcgcgt ccagcacggt gaagccctcg accagcgagg cgagcgcgcc ggcggtcgta
      661 tggtggatgt ctatgacgcc gtcgcgcacc atcgccagct gttccgccgt gtcgccgagg
      721 accgacgcgg ggaatacctc gacgacgagc ctgccttcgc tcagctcgcg cgcgcgtctg
      781 gcgaagtact cggcttgcat gcccgctacg ctgtccagcg ggctcatgtg ggcgtagcgt
      841 agcgtgacga tggggcgctg gtcgcaggcg gcggccgata cggccacggc catagccatc
      901 gcggcgagcc tcgtcccggc cgtcatcggg ccttcccgtc ggcggacgag tcgaagcgga
      961 ccgtcacgcg ggtgccgccc ccgctcgggc gctcccaggc gacggtcccg cggagctgat
     1021 cggcaaggcc ggcgatcagt gagatgccga gcgagccctt gtcggccacc tcgggcggca
     1081 ggccgggtcc gtcgtcctcg acgaccaggc agaccatcct gtcccgccca gcctcgtcca
     1141 gcgacaagct gacgcggacc ctgccatcgg ttcccggcgg tatgccgtac ttcagcgcgt
     1201 tcgatacgat ctcgccgagg agcagtccga agggcacggc tcgctcgagg ctcagcggca
     1261 tcggttcgta ggcggccagg acgctatcgc ggggtacctt gtactcgtcc gccaggtagg
     1321 cgacgaggtg ggacgcgtat tcgcccgcgt cgaccgagga gatgtccggc atctgataga
     1381 cgatctcgtg ggccagcgac atcgcgaaga ccctgtcctg ggcgccctgc agcgccgtcg
     1441 acacggatcg gtccgcggtc acggccgcct gcatggacaa aaggctcgag atgacctgga
     1501 ggttgttctt gacgcggtgg tagacctcgc gcaggagcag ggtcttctcc tcgagggccg
     1561 cgacgctacg ttcctcggct atcctctggt cggtcacgtc gaagaccaga gcgtggagca
     1621 gctcctcgtc gccgagctcg agcgggctga cgtacaggat cacgtcgcgt atctccccg
//
LOCUS       DAOOLN010000441         1678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_92991_length_1678_cov_2.572397, whole genome shotgun sequence.
ACCESSION   DAOOLN010000441 DAOOLN010000000
VERSION     DAOOLN010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92991_length_1678_cov_2.572397"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLW80_06280"
     CDS             complement(<1..255)
                     /locus_tag="PLW80_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB66146.1"
                     /translation="MAFIIEMLNIVKDFPGIRANDDVTLQVEKGSIHALLGENGAGKS
                     TLMSILFGLYSPDGGSIRIRGETVRISDPNVANELGIGMVH"
     gene            complement(528..1574)
                     /locus_tag="PLW80_06285"
     CDS             complement(528..1574)
                     /locus_tag="PLW80_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPB66147.1"
                     /translation="MKYRTFFAVLLIAALAFTGCAKEEKAAPVAAKPAFKVGLVTDLG
                     GIDDKSFNQGTWEGVIRFGEENGLVKGTDYKYLQSGAEADYVPNLSTFADEKLDLIVA
                     PGFLFANAMGEVAEKYPQQKLMIIDDSSVQKPNVVCAVFAEHEGSYLVGVATGLKAKA
                     DGKKVVGFIGGMQFPLIEKFQAGFEQGVKAVYPEAKILVDYAGDFGAPDKGQALAQKQ
                     FNAGAYIIFHAAGGTGNGMIKEAKERSEKGDVRWAIGVDKDQYSDGLYGDKSAVLTSM
                     MKRVDVAAYEVAKMTLAGQFPGGQTLVFSLKNNGVGIPNENPNLSADIVASVNEYAKK
                     IASGELVVSEIPAK"
BASE COUNT          298 a          510 c          532 g          338 t
ORIGIN      
        1 atgcaccatg ccgatgccca gctcgttggc tacgttcgga tcgctgatgc ggacggtctc
       61 cccgcggatc cggatgctgc ctccgtcggg cgaatacagc ccgaatagga tgctcatcag
      121 cgtcgattta ccggcgccgt tctcgcccag aagggcatgg atgctgccct tctctacctg
      181 gagcgtcacg tcgtcgttag cgcggatgcc cgggaagtct ttgacgatat tgagcatctc
      241 gatgatgaac gccatcgcgt gcccccattg tgtcatctgg gattcttccg cgccgggatc
      301 acccgcgcgg gtcatacaga atactataaa taaagctctt tgggaaggaa ccgtcgggcg
      361 accggcggac gacgcgccgc gccttcgtca tagaaacgaa gagcggccca gtagccgcgg
      421 cgagcgcacc atcggtacgc ctgtcgcgcc actagccgct cacgtgacgg ataccgtcgg
      481 cgctacccgg cgcccggagg cgacggatag cgcgataggg aggcgaatta cttcgcgggg
      541 atttccgaga cgacgagctc gccggaggcg atcttcttgg cgtactcgtt gacggacgcg
      601 acgatgtcgg ccgacaggtt cgggttctcg ttcgggatgc cgacgccgtt gttcttcagg
      661 gagaacacga gggtctggcc gccggggaac tggccagcca gggtcatctt cgcgacctcg
      721 taggcggcca cgtcgacgcg cttcatcatc gaggtcagca cggcggactt gtcgccgtag
      781 aggccgtcgg agtactggtc cttgtcgacg ccgatagccc agcggacgtc gcccttctcc
      841 gagcgctcct tggcttcctt gatcatgccg ttgccggtgc caccggccgc gtggaagatg
      901 atgtaggcgc cggcgttgaa ctgcttctgc gcgagagcct ggcccttatc cggggcgccg
      961 aaatcgccgg cgtagtcgac gaggatcttg gcctcggggt agacggcctt gacgccctgc
     1021 tcgaaaccgg cctggaactt ctcgatgagc gggaactgca tgccgccgat gaaaccgacg
     1081 accttcttgc cgtcggcctt ggccttgagg ccggtcgcga cgccgacgag gtaggaaccc
     1141 tcgtgctcgg cgaagacggc gcagaccacg ttcggcttct gcacgctgga gtcgtcgatg
     1201 atcatcagct tctgctgcgg atacttctcg gcgacctcgc ccatggcgtt ggcgaagagg
     1261 aagccgggcg cgacgatcag gtcgagcttc tcgtcggcga aggtgctgag gttcggcacg
     1321 tagtcggcct cggcgccgga ctggagatac ttatagtcgg ttcccttgac gagaccattc
     1381 tcctcgccga aacggatgac gccttcccag gtcccctggt tgaaggactt gtcatcgata
     1441 ccgccgagat cggttacgag gccgaccttg aacgccggct tggcggcgac cggagcggcc
     1501 ttctcttcct tggcgcaacc ggtgaaggcg agcgcggcga tgagaagcac ggcgaagaaa
     1561 gttcgatact tcatactaac tcctttactt tttcgctatc gcgaaaatgt ggaactcgat
     1621 agtaacccct tacgcgattc agtgcaagtc ggtattacac ctttttgagc gtatcgcg
//
LOCUS       DAOOLN010000442         1677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_93110_length_1677_cov_2.139334, whole genome shotgun sequence.
ACCESSION   DAOOLN010000442 DAOOLN010000000
VERSION     DAOOLN010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93110_length_1677_cov_2.139334"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PLW80_06290"
     CDS             complement(<1..345)
                     /locus_tag="PLW80_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66148.1"
                     /translation="MSRKELEAALDFILNRADPSEFEVLAKACERRRRDMGKYAGLGG
                     LNPGALADKMAASVQEGVGRSLDGLRDTLRDYVARLVRQKEPGATDEQIEALLDECLP
                     DRSDPSSGARDVP"
     gene            487..>1677
                     /locus_tag="PLW80_06295"
     CDS             487..>1677
                     /locus_tag="PLW80_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66149.1"
                     /translation="MPAKRDFGVTLDRRSPSERGRLIAYINLKLSALGLPVYGKEGTA
                     FLELASDMLLNYREKARLLADYLPPADARIQAFLDSYLADLPDGEAPRLPSNTLTLDR
                     YGMARELSLPPDAHEYRSPTMSSYRIRNGILHNPKNDRRTTKGVFHIAEGGLPVPLDK
                     LAVPKLAFARMLTAALAPPDELLVLPFTAQEAEPARCFLSLLMRPTVRPEVPGLYEER
                     SMETRFFAPASLAANLDFVESIFGNSGDPYVSANDAALDPLHWSGHTGCVILATHLVG
                     MPKKALGLPSWDEATERQRRDGMAWKDPSEPYNGGQPFKLCARDERGVIVTIIADNYF
                     GYSKKEIKTQISYSSNLLGLTEEEHAGGALVLPSFNLGNRFVPDTNLRSKGQRFDDVV
                     RLLGD"
BASE COUNT          293 a          603 c          504 g          277 t
ORIGIN      
        1 tggaacgtcc cgggctcccg acgaagggtc ggacctgtcg ggcaggcatt cgtcgagcag
       61 cgcctcgatc tgctcgtcgg tcgcgccggg ctccttctgg cgaacgaggc gggccacgta
      121 gtcgcgcagg gtgtcgcgca gcccgtccag gctacggccg acgccctcct ggaccgaggc
      181 ggccatctta tcagccaggg cgccgggatt gagaccgcca agcccggcgt acttgcccat
      241 atcccgccgc ctgcgctcgc aggccttggc gaggacctcg aactcggacg ggtccgcccg
      301 gttcaggatg aagtcgagcg ccgcttccaa ttctttcctg gacataacgc cactatacca
      361 tcgttcgatc gccgagtctg tcgtcgaccg ggcgactagg cgccctcctg gcaaaaagtc
      421 gtcgttcata ctacgatgga ccggaggccc tgacggcccg tcccatacgc atgaacggag
      481 gctcatatgc cggcaaaacg agacttcgga gtcaccctgg atcgacgctc gccgtcggaa
      541 cggggaaggc tcatcgcata catcaacctg aagctatcgg cactgggcct cccggtctac
      601 ggcaaggaag gtaccgcgtt ccttgagctg gccagcgaca tgttgctcaa ctaccgggag
      661 aaggcgcggc tgctcgccga ctatctccct ccggcagacg cgcgcatcca ggcgttcctc
      721 gactcgtacc tggccgacct gcccgacggc gaggcgcctc gcctgccctc gaacacgctg
      781 acgctcgacc ggtacggcat ggccagggag ctgtcgctgc cgccggacgc ccacgagtac
      841 cgctctccca cgatgtcgtc gtataggata cgtaacggta tcctccataa tcccaagaac
      901 gaccggcgca cgaccaaggg cgtcttccat atagccgaag gcggcctgcc ggtcccgctc
      961 gacaagctgg ccgtgcccaa gctagccttc gccagaatgc ttaccgccgc gctggccccc
     1021 ccggatgaac tcctcgtcct accgttcacg gcgcaggagg cggaaccagc ccgctgcttc
     1081 ctgtccctcc tgatgcggcc tacggtacgg cccgaggtgc ccggtctcta cgaggaacgg
     1141 tcgatggaga cgcgcttctt cgccccggcc tcgctcgcgg ccaacctcga cttcgtcgag
     1201 agcattttcg gcaactcggg cgacccctac gtctcggcca acgacgccgc gctcgacccg
     1261 ctccattggt cgggccacac cggctgcgtc atcctggcca cgcacctggt cggcatgccc
     1321 aagaaggcgc tcggcctgcc atcctgggac gaagccaccg agcgccagag gcgcgacggc
     1381 atggcctgga aggatccgtc cgaaccgtac aacggcggcc agccgttcaa gctctgcgcc
     1441 cgcgacgagc gcggtgtcat cgtcaccatc atcgcggata actacttcgg gtactccaag
     1501 aaggagatca agacgcagat ctcctactcg tcgaacctgc tcggcctgac cgaggaggag
     1561 cacgccggtg gcgccctcgt gctgcccagc ttcaatctcg gcaaccgctt cgtgccggat
     1621 accaacctgc gctcgaaggg ccagcgcttc gacgacgtgg tgcgcctgct cggcgac
//
LOCUS       DAOOLN010000443         1672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_93515_length_1672_cov_2.756339, whole genome shotgun sequence.
ACCESSION   DAOOLN010000443 DAOOLN010000000
VERSION     DAOOLN010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93515_length_1672_cov_2.756339"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="PLW80_06300"
     CDS             complement(<1..1253)
                     /locus_tag="PLW80_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66150.1"
                     /translation="MERAGRGSLTRGGPRWTALAIAAGVSATLGVAAIVAIALSLRPG
                     SDRSFGALLARIDASPVTASAEAYLNAAALAKGSEDRLRVLKRAALRDSALYADVAAV
                     MTGSPVSQPVALAALDAYLKAGRFVEALSLFDGPLDPRTNAAALAETVVTAARAGRAL
                     SPSIDQLVACFDATGDSSFLTSAAIDAMRKGDRQAASMLLRDAELAPYRLLWDAMALE
                     PLARRVPVSEEPLELAVRADAERLLGETVAATASYAFIVTNYPDWSWKPYASLARLCG
                     VEGASAAPRWPYSAVPGSSESLASPTALAERLHGEMLGRFSDDSDAVIERASWLYDND
                     RSAEASRLLVGLEGERASIARLAYGSPDRAVPDAARLAATYPLSAAAVDAALGAFASA
                     GSWDRFVAALGEASAAELPLPRLWF"
     gene            complement(1226..1651)
                     /gene="nusB"
                     /locus_tag="PLW80_06305"
     CDS             complement(1226..1651)
                     /gene="nusB"
                     /locus_tag="PLW80_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPB66151.1"
                     /translation="MASRRKARILAFQAIYAWDASSVGYDELLSFSWLGEDSLASSDE
                     ELKAFAGLIVSGTIENVEAVDAAIKRHLEHWAFERLKRVDLAILRVSVYSLVYQRDIP
                     AQITIDEAIEIAKEFGSEDSYRFINGVLDAVWKERGAEA"
BASE COUNT          291 a          567 c          571 g          243 t
ORIGIN      
        1 cagaaccaga gcctcggtaa cggaagctcc gccgccgagg cctcgcccag agcggcgacg
       61 aagcgatccc acgagccggc gctcgcgaac gcgccgaggg ccgcgtctac ggcggcagcc
      121 gatagcgggt atgtcgccgc gaggcgagcg gcgtccggca cggcccgatc gggagagccg
      181 tacgccagcc tggcgatgga agcccgctcg ccttcgaggc cgacgaggag gcgcgaggcc
      241 tctgccgacc tgtcgttgtc atacagccag gaagcccgct cgataacggc gtcgctatcg
      301 tccgagaaac ggccgagcat ctcgccgtgg aggcgttcgg ctagcgccgt cgggctggct
      361 aggctctcgg acgagccggg cacggccgaa tacggccagc gcggcgccgc agaggcgccc
      421 tcgacgccac agaggcgcgc cagcgacgca tacggcttcc atgaccaatc ggggtaattc
      481 gtaacgatga acgcgtagga tgccgtggcg gccacggttt cgcccaagag ccgttccgcg
      541 tcggctcgta ccgccagctc gagcggctcc tccgataccg ggacccgccg agccaggggc
      601 tcgagcgcca tcgcgtccca tagaaggcga tacggggcga gctccgcgtc gcgcaggagc
      661 atgctagcgg cttgacggtc gcccttccgc atggcgtcga tcgcggcgct cgtcaggaac
      721 gacgagtcgc cggtcgcgtc gaagcaggcg accagctggt cgatcgatgg gctgagcgca
      781 cggccggcgc gcgcagccgt gacgacggtc tcggcgagcg cggcggcgtt ggtcctagga
      841 tcgaggggac cgtcgaagag cgacagcgct tcgacgaaac ggccggcttt aaggtaggca
      901 tcgagcgcgg ccagggccac cggctgcgag accgggcttc ccgtcatgac cgcggccaca
      961 tcggcgtaga gggcgctatc gcggagcgcc gcccgcttca gcacgcgcag gcggtcctcc
     1021 gagcccttcg ccaaggcggc ggcattcagg taggcttccg ccgaggccgt cacgggcgac
     1081 gcgtctatcc tggccaacaa ggcgccgaag gatctatcgg agcctggccg tagcgatagc
     1141 gcgatcgcga cgatcgccgc gacgccgagg gtggcggata cccccgccgc gatcgcgagc
     1201 gcggtccatc gcgggccgcc ccgcgtcagg cttccgcgcc ccgctctttc catacggcat
     1261 ccagcacgcc gtttatgaag cggtacgaat cctcggagcc gaattccttc gcgatctcga
     1321 tcgcctcgtc aatcgttatc tgggccggta tgtcccgttg gtaaacgagc gaatagaccg
     1381 acacgcgcag gatggctaga tcgaccctct tgagccgctc gaaggcccaa tgctcgaggt
     1441 gccgcttgat cgcggcgtcg acggcttcga cattctctat cgtacctgag acgatgagcc
     1501 cggcgaaagc cttaagctcc tcgtcgctac tggccaggct atcctcgccg agccacgaga
     1561 aggacaggag ctcgtcgtag ccgacggagg aggcatccca agcgtagatg gcttggaacg
     1621 cgagtatcct cgccttacgc ctcgacgcca tcagggagtc cccaggatct tg
//
LOCUS       DAOOLN010000444         1671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_93604_length_1671_cov_2.813119, whole genome shotgun sequence.
ACCESSION   DAOOLN010000444 DAOOLN010000000
VERSION     DAOOLN010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93604_length_1671_cov_2.813119"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1474
                     /locus_tag="PLW80_06310"
     CDS             <1..1474
                     /locus_tag="PLW80_06310"
                     /inference="COORDINATES: protein motif:HMM:NF018168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HPB66152.1"
                     /translation="PSELAPREAVVQRGKTLIDSIHGQYQRLSATRDMVQDDIEISAA
                     QANDLSRQIAALNAEIIKSEAMGDNPNDLYDRRDVLVEKLATIVPVSVDRRDPDEFMV
                     HVDGHILVQGKIARGFELSTGAESGGYPRVLWADTGDEAVIPGGSLGAMLEMRDVDLK
                     EEIASLDTMTLTFTDLVNEVHRSAYGLNGKTGVDFFTELPSVNNIAGNYDRDGDGAYD
                     HSYVYRLSGGNALDAQEQLGLAGTMRLSGPRGLVDVEYRPTDTVGDLVRRINDSGADV
                     TARLTSTGALQLRAKTASDGESPDFVIRHVEDSGEFLVGYAGLLAASGPDAAYSWDRA
                     DAVTGLRGGVDYAVAPLAHPSGWIEVSSAVTRDLTSVAAGFGSNGRPAEAGDGSAAIA
                     IARLRVAPVMVGSQKTFDDYFADAVASVGLKGERAGRMADTQSRVVKNLTDTRDSISG
                     VNMDEELSNMIKFQHGYSAAAKFVSTMDSMLDTIINRLRG"
BASE COUNT          293 a          597 c          525 g          256 t
ORIGIN      
        1 cccctcggag ctcgccccgc gcgaggccgt cgtccagcgc ggcaagaccc tcatcgactc
       61 gatccatggc cagtaccagc gcctgtcggc cacccgcgac atggtccagg acgacatcga
      121 gatcagcgcc gcccaggcca acgacctgtc caggcagata gccgcgctca acgccgagat
      181 catcaagagc gaggcgatgg gcgacaaccc gaacgacctc tacgaccgcc gcgacgtgct
      241 cgtcgagaag ctggcgacga ttgtacccgt ctcggtggat cgccgcgacc cggacgagtt
      301 catggtacac gtcgacggcc atatcctggt gcagggcaag atcgcgcgcg gcttcgagct
      361 gtcgaccggc gcggagtcgg gcggctaccc gcgcgtgctc tgggccgaca ccggcgacga
      421 ggccgtcata cccggcggct cgctcggcgc catgctcgag atgcgcgacg tcgatcttaa
      481 ggaagaaata gcctcgctcg acacgatgac gcttaccttc accgacctcg tcaacgaggt
      541 gcaccgctcc gcctacgggc tcaacggcaa gaccggcgtg gacttcttca ccgagctgcc
      601 gagcgtcaac aacatagcgg gcaactacga ccgcgacggc gacggcgcct acgaccacag
      661 ctacgtgtac cgcctgtcag gcgggaacgc ccttgacgcg caggagcagc tcggcctggc
      721 cggcacgatg cgcctgtccg gcccccgggg cctcgtcgac gtggagtacc gaccgaccga
      781 caccgtcggc gacctggtcc gccgcatcaa cgactcgggc gccgacgtga cggcccggct
      841 gacctcgacc ggtgccctgc agctgcgcgc caagaccgcc tccgatggcg agagccccga
      901 cttcgtcatc cggcacgtcg aggactccgg cgagttcctc gtcggctacg ccggcctgct
      961 cgcggcctcg ggacccgacg ccgcctattc ctgggaccgc gccgacgcgg tgaccggcct
     1021 gcgcggcggc gtcgactacg ccgtggcccc gctcgcccac ccgtccggct ggatagaggt
     1081 gtcgagcgcc gtcacccgcg acctgacgag cgtcgcggcc gggttcggct ccaacggccg
     1141 gcccgccgag gccggcgacg ggagcgccgc catcgcgata gccaggctgc gcgtcgcgcc
     1201 ggtcatggtc ggctcgcaga agaccttcga cgactacttc gccgacgccg tagcctcggt
     1261 cgggctcaag ggcgagcgcg cgggccgcat ggccgacacg cagtcccgcg tcgtcaagaa
     1321 cctgaccgat acgcgcgact ccatctccgg cgtcaacatg gacgaggaac tgtccaatat
     1381 gatcaagttc cagcacggct actccgcggc ggccaagttc gtgtcgacga tggactcgat
     1441 gctcgacacc atcatcaacc gcctgcgcgg ctagcgtttg cgtactaaaa gtcttggcca
     1501 cggaggcacg gagacacgga gaggagggag agatgactag gagtggaatc aagggttcca
     1561 gttttcatgc ttctattcat cttcatcccc cttatgttct tcctccgcgc ctccgtgtct
     1621 ccgtggttca aatacctatc tatcgcagtc ccagggggtt cagatgcgta g
//
LOCUS       DAOOLN010000445         1670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_93678_length_1670_cov_3.293498, whole genome shotgun sequence.
ACCESSION   DAOOLN010000445 DAOOLN010000000
VERSION     DAOOLN010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93678_length_1670_cov_3.293498"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1670
                     /locus_tag="PLW80_06315"
     CDS             <1..>1670
                     /locus_tag="PLW80_06315"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPB66153.1"
                     /translation="LASLSFEAAAAYVDELDRTWLGSLCVELTVAASDDRAVAQVMLS
                     DSPSFLNASWVTFNAAAPVYELVLGGMDGPKRLYGKVKDGAGNVSDVRVSEEVHLDLE
                     PPTIDNLLINGGASATNSGLVSLKVFSGDDEGASSGLHRFRYRPANGSWSDWLDLSLD
                     SGAGAGEVSNVATNAELGGSAVFEAQVIDRVGHVSAVASRTIRYEQTPPTVIGVSWDD
                     QALFPYNGSVLRIQFDEEMRPDSFPEGSFTLARTAGGAIDGTVNLTASGATPNATAEL
                     WGLELYPNTSYLVTLSGAVQDIAGNPLGADKTWYFSTGDAEDTSPPTGAVTLTDQGGA
                     VIVVTLPSGSIATDDATLELDFSAITDDYNIPYGIKIWGDNGNAAAFFEEDASWRTWS
                     DTVSWTLSAGSGTKYVLYKLMDSANNQSVTPRQLRIILDSGELPTIATVSVNGGQSHT
                     NASDRAVDLTIEASDAYSGVKQMIISHDPSFAGASWMNYTPILEGWVLPDGDGTRIFY
                     AKVRDYLDQESATNQACSITLDRAAPSVSFNVESILASEPTLLAEGSD"
BASE COUNT          315 a          556 c          528 g          271 t
ORIGIN      
        1 ccctcgcctc gctgtcgttc gaagcggcgg ccgcgtacgt cgacgagctc gataggacct
       61 ggctcggctc cctctgcgtt gagcttaccg tggccgcctc ggacgaccgg gccgtcgcgc
      121 aggtgatgct atccgacagc ccgtcgttct tgaacgcctc ctgggttacc ttcaacgccg
      181 ccgctccggt ctatgagttg gtactcggcg gcatggacgg gccgaagcgc ctctacggca
      241 aggtcaagga cggcgccggc aacgtcagcg acgtacgcgt gtccgaggaa gtccacctag
      301 atctagagcc gcccacgatc gacaacctgc tcatcaacgg cggcgctagc gccaccaact
      361 ccggcctggt cagcctcaag gtcttctcgg gcgacgacga gggcgcctcg agtggcctcc
      421 ataggttccg ctaccggccg gcgaacggga gctggagcga ttggctcgac ctatcgctcg
      481 atagcggcgc tggcgctggc gaggtgtcca atgtagccac gaacgccgag ctaggcggga
      541 gcgcggtatt cgaggcgcag gtgatcgaca gggtgggaca cgtatccgcg gtagcgtcca
      601 ggacgatacg gtacgagcag acacctccca ccgtcatagg cgtcagttgg gacgaccagg
      661 cgctcttccc gtacaacggc agcgtgctgc gcatccagtt cgacgaggag atgaggccgg
      721 acagcttccc cgagggcagc tttacgctcg cgaggacggc cggcggcgcc atagacggca
      781 ccgtcaacct gaccgcctcc ggggcgactc cgaacgcgac ggccgagcta tggggcctcg
      841 agctgtaccc gaacacctcg tacctggtga cgctctcggg ggcggtccag gacatagccg
      901 gcaaccccct cggcgcagac aagacctggt acttcagcac cggcgacgcc gaggacacga
      961 gcccgccgac cggcgcggtc acgctgaccg accaaggcgg cgcggttatc gtcgtcacgt
     1021 tgccgtccgg gtcgatagcc accgacgacg cgacgctcga gctggatttc tcggccatca
     1081 cggacgacta caacatcccc tacggcatca agatatgggg cgacaacggc aacgccgctg
     1141 cctttttcga ggaagacgcg agctggagga cctggtccga caccgtatcg tggacgctat
     1201 cggccggctc gggaaccaag tacgtgctct acaagctgat ggactcggcc aacaatcagt
     1261 ccgtcacgcc gcgtcagctc aggatcatcc tggactccgg cgagctcccg acaattgcga
     1321 ctgtttcggt gaacggcggt cagagccata cgaacgcttc ggaccgggcc gtcgacctta
     1381 ccatcgaagc gagcgacgcc tatagcggcg tcaagcagat gataatctcg catgacccta
     1441 gcttcgctgg cgcctcgtgg atgaactata cgccgatctt ggaaggctgg gtcttgccgg
     1501 acggcgacgg aacccgcatc ttctacgcaa aggttcgcga ctatctggat caggaatcgg
     1561 ccaccaatca ggcctgctcg atcacgctgg accgcgccgc gccgagcgtc tcgttcaacg
     1621 tcgagagcat cctggccagc gagccgaccc tgctcgccga gggcagcgat
//
LOCUS       DAOOLN010000446         1670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_93701_length_1670_cov_2.437771, whole genome shotgun sequence.
ACCESSION   DAOOLN010000446 DAOOLN010000000
VERSION     DAOOLN010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93701_length_1670_cov_2.437771"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PLW80_06320"
     CDS             complement(<1..631)
                     /locus_tag="PLW80_06320"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPB66154.1"
                     /translation="MAELPASIGKYPIDALFARGGMGAVFKATHPTLKRHVVLKKLTM
                     RGSASIAERFKREARILMDFKHDNIVRVFDHFKEGSSHYMVMEFVDGESLDALIRRRR
                     FLSTELSLAIFLKACRALAYAHERGVVHRDIKPGNILISKKGEVKLADFGIASSGDED
                     DGGLTREGMTLGTPSYMPPEQIENAKNVDKRADVYAMGIMLYEMTTGKKP"
     gene            complement(603..1229)
                     /locus_tag="PLW80_06325"
     CDS             complement(603..1229)
                     /locus_tag="PLW80_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66155.1"
                     /translation="MILKAAFPRAFHFLKGNHENVLDEEGDGNHSFGKFAEEGAMVAE
                     YMLGAYGRELTESYAAFEKALPLFAVGDRFLASHAEPARAYSDEELVDAPNRPEVILG
                     LTWTDNGAAEPGAVGALLDRILPNVPDARYITGHRPVPAPLRYLERSDGRHLQIHAPD
                     SFNVAWVMPDRALAPELDVGAIPDISSSLPRQAGGQRRTHGGATRIDR"
     gene            complement(1226..>1670)
                     /locus_tag="PLW80_06330"
     CDS             complement(1226..>1670)
                     /locus_tag="PLW80_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="calcineurin"
                     /protein_id="HPB66156.1"
                     /translation="PAEARERLRESFLRPGRPDRDRLRAVVEAAAGALAAEPAERRPP
                     ASDGRPGGLVRLLSGVPTIVVPDLHARGAFILSLAAMPLPDGGDICGALAEGALQVLC
                     LGDGFHAEARAAGRWRDAYREFLGGYTESTAMDDEMRESLSQRWS"
BASE COUNT          250 a          558 c          584 g          278 t
ORIGIN      
        1 agggcttctt gccggtcgtc atctcgtaga gcatgatgcc catggcgtag acgtcggctc
       61 gcttgtcgac gttcttggcg ttctcgatct gctccggcgg catgtacgac ggcgtgccga
      121 gcgtcatgcc ctcgcgggtt aggccgccgt cgtcctcgtc gcccgaggag gcgatgccga
      181 agtcggcgag cttcacctcg cccttcttcg atatcaggat gttgcccggc ttgatgtccc
      241 ggtggacgac gccgcgctcg tgggcatagg cgagcgctcg gcaagccttc aggaagatgg
      301 ccagcgatag ctccgtcgat aggaagcggc gacggcggat cagcgcgtcg agcgactcgc
      361 cgtcgacgaa ctccatcacc atgtagtgcg acgaaccttc tttaaagtgg tcgaagactc
      421 gcacgatgtt gtcgtgcttg aagtccatca ggatcctggc ctcgcgcttg aagcgctccg
      481 cgatggacgc ggagccgcgc atggtcagct tcttgaggac gacgtggcgc ttgagcgtcg
      541 ggtgggtcgc cttgaacacg gcccccatgc cgcccctggc gaacaaggcg tctatagggt
      601 acttaccgat cgatgcgggt agctccgcca tgcgtcctcc tctgtcctcc cgcctggcgc
      661 gggaggctcg acgaaatatc cggtatggcg cccacgtcca gctcgggcgc gagggcgcgg
      721 tcgggcatga cccacgccac gttgaacgaa tcgggagcgt ggatctgcag gtgacgcccg
      781 tctgagcgct ccaggtagcg tagcggcgca ggcaccggcc ggtggccggt gatgtagcgg
      841 gcgtcgggaa cgttcggcag gatacggtcg agcagggcgc ccacggcgcc cggctcggcg
      901 gccccgttgt cggtccaggt caggccaagg atgacctcgg gccggttcgg cgcgtcgacg
      961 agctcctcgt cggagtaggc gcgcgccggc tcggcgtgcg aggcgaggaa gcgatccccg
     1021 accgcgaaca gcggcagggc cttctcgaag gcagcgtacg actcggtcag ctcccgtccg
     1081 taggccccga gcatgtactc ggcgaccatc gcgccctctt cggcgaactt gccgaaggaa
     1141 tggttgccgt cgccttcctc gtcgagcacg ttctcgtggt tgcccttgag aaagtggaag
     1201 gcccgcggga aggccgcctt gaggatcatg accatctctg cgacaggctc tcgcgcatct
     1261 cgtcgtccat cgccgtcgat tccgtgtaac cgcctaaaaa ctcgcggtac gcgtcgcgcc
     1321 agcggcccgc tgccctggcc tccgcgtgga agccgtcgcc gaggcatagc acctggagcg
     1381 cgccctcggc cagcgccccg cagatgtctc cgccatcggg cagaggcatg gccgccaggc
     1441 tgaggatgaa ggcgcctcgc gcgtgcaggt cgggcaccac gatggtcgga acccctgaca
     1501 ggagccgtac gagacccccg ggccggccgt ccgacgccgg cggccggcgc tcggcaggct
     1561 cagcggccag ggcgccggcc gcggcctcga cgacggcgcg gagccggtct ctgtcggggc
     1621 ggccgggcct caggaacgat tcgcgcaggc gctcgcgcgc ctcggccggg
//
LOCUS       DAOOLN010000447         1669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_93760_length_1669_cov_3.602230, whole genome shotgun sequence.
ACCESSION   DAOOLN010000447 DAOOLN010000000
VERSION     DAOOLN010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93760_length_1669_cov_3.602230"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..887)
                     /locus_tag="PLW80_06335"
     CDS             complement(15..887)
                     /locus_tag="PLW80_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HPB66157.1"
                     /translation="MLILLADAFKADLPKRLEAYGEVTQDQGRVADAEIIIVRSKTKV
                     DKAMMDKAPKLKFIIRGGVGVDTIDVEYAKSKNVAVDNTPEASSLAVAELAFALMISL
                     PNHIAEADASMKQGKWLKKELERTELAGKTLGLVGIGRIAREVAVRAKAFGMRVIAYD
                     KFVEKSDAAEMMSLEQVLAQSDYISLHTPLTDETKGMINAKALASMKKGAYLVNTCRG
                     KVVVEEDIVAALESGQLAGYGTDVYYSDPPENTPLAKAPHVVMTPHLGASTAENLDRL
                     GDCIVERVRKYCKK"
     gene            complement(1128..>1669)
                     /locus_tag="PLW80_06340"
     CDS             complement(1128..>1669)
                     /locus_tag="PLW80_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66158.1"
                     /translation="RRPLPGNRSYGYSRLMQLHLSMRRSGTALIYGIPLWYRLTASAI
                     ALTLAISSSISGAPSLGALVVVAVAAVAALYQERWSFDAASGECSGRVGFVFAARGPS
                     FKADEVARLRLDTFAKGRLDQRDLPPDDKLPRGSQARLLVDLKDGESYMIDSVPVKRR
                     PELELNARAIAEALGASLE"
BASE COUNT          252 a          519 c          582 g          316 t
ORIGIN      
        1 atggtatagc ctccttattt cttgcagtac ttccgtacgc gctcgacgat gcagtcgcca
       61 agccggtcca ggttctcggc cgtcgaggcg ccgaggtgcg gggtcatgac gacgtggggc
      121 gccttggcga gtggcgtgtt ctccggcggg tcggaatagt acacgtcggt gccgtagccg
      181 gccagctggc cgctctcgag cgcggcgacg atgtcctcct cgacgacgac cttgccgcgg
      241 caggtgttga cgaggtaggc gcccttcttc atcgaggcga gggccttggc gttgatcatg
      301 cccttggtct cgtcggtcag cggggtgtgg aggctgatgt agtcggactg ggccagcacc
      361 tgctccaggc tcatcatctc cgcggcgtcc gacttctcga cgaacttatc gtaggcgatg
      421 acgcgcatgc cgaaggcctt ggcgcggacg gcgacttcgc gcgcgatgcg gccgatgccg
      481 acgaggccga gggtcttacc ggccagctcg gtacgctcga gctccttctt gagccacttg
      541 ccctgcttca tcgacgcgtc ggcctcggcg atatggttcg gcagcgatat catcagcgcg
      601 aaggccaact cggcgaccgc gaggctcgag gcctcggggg tattatcgac cgcgacgttc
      661 ttggacttgg cgtactcgac gtctatcgtg tcgacgccga cgccgccacg gatgatgaac
      721 ttgagcttcg gagccttgtc catcatcgct ttgtcgacct tggtcttcga gcggacgatg
      781 atgatctcgg cgtcggcgac gcggccctgg tcctgggtca cctctccgta cgcctcgagg
      841 cgcttcggca ggtcggcctt gaaggcgtcg gcgagcagta taagcatggg atcctccttt
      901 gttgtatcgt cgctcgatcg tagcgcgtct ctggcgatgg acagtccgcc ccgggaggcc
      961 ggtgatggcc cggggcgaga cgaacccatc atggattcgt gctatgagtg ggacgatgca
     1021 gtcatcttat catggaagga agccttgtca agccgggcgg ccggcgaccg atggtaggcc
     1081 gacgccggcc gccgctattg gccgacgccg gcggctcctg gccgccgcta ttccaggctc
     1141 gctcccagcg cctcggcgat ggcgcgggcg ttcaactcga gctccggcct gcgcttgacc
     1201 ggcaccgagt cgatcatata gctctcgccg tccttcaggt cgacgagcag ccgcgcctgg
     1261 gagccgcgcg gtagcttgtc gtccggcggc aggtcgcgct ggtcgagcct gcctttggcg
     1321 aaggtatcca gcctgaggcg ggctacctcg tcggccttga acgaagggcc gcgggcggcg
     1381 aagacgaagc ccaccctgcc ggagcactcg ccgctcgcgg cgtcgaagct ccagcgctcc
     1441 tgatagagcg ctgccaccgc ggcgacggcc acgacgacga gcgcgccgag gctcggggcg
     1501 ccggagatgg acgatgagat ggctagcgtc agcgcgatgg cggatgcggt tagacggtac
     1561 caaaggggga tgccgtagat cagcgcggtt ccggatcggc gcatggagag atggagttgc
     1621 atgagtcgac tatagccgta gctccggttc ccgggcaagg gccggcgct
//
LOCUS       DAOOLN010000448         1669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_93764_length_1669_cov_3.241636, whole genome shotgun sequence.
ACCESSION   DAOOLN010000448 DAOOLN010000000
VERSION     DAOOLN010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93764_length_1669_cov_3.241636"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1669)
                     /gene="lon"
                     /locus_tag="PLW80_06345"
     CDS             complement(<1..>1669)
                     /gene="lon"
                     /locus_tag="PLW80_06345"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPB66159.1"
                     /translation="GDDGGPSGADGRKELEALVQILRRDFSTYADLVKKIPNETMLAV
                     QRAESPHKLCGTIANAMGLKLERKQELLAVVPLRERLEATAKALSQDIELLSIQKRIS
                     QKVRARMEKSQKDYFLQEQLKEIHKELGRDDDESDLAELTRRIESKAPPQEVLDKAKK
                     ELARLAKLQPLSPEAGVIRGYCEWLADLPWSARKADSKDLAAAKAALDEDHYGMRKAK
                     ERILEFIAVRQLNDSLKGPILCLVGPPGTGKTSLGRSVARALGRDFVRMSLGGVRDEA
                     EIRGHRKTYVGALPGKILQSMRKAGSSNPVFLLDEIDKMSSDFRGDPASALLEVLDPE
                     QNASFVDHYLEVPYDLSGVMFVTTANSVHSIPYALLDRMELIEIPGYSEYEKLEIARH
                     FIVPRQIRENGLEGSSTRIRDDAVLEIIRHYTMESGVRSLEREIARVVRKTAERAVEA
                     GYAGGQRPLAEFKRVIGASSLRKLLGKRRHENDLVFSEPGPGLACGLAWTEMGGSVLP
                     VEASSFEGEGELILTGNLGDVMKESARAALTYLRAHAEEYGLSPTDFA"
BASE COUNT          243 a          546 c          540 g          340 t
ORIGIN      
        1 agcgaagtcc gtcggcgaca gaccgtactc ctcggcgtgg gcccgcagat aggtcagcgc
       61 cgccctggcg ctctccttca tcacgtcgcc gaggttgccc gtcaggatca gctcgccctc
      121 cccctcgaag ctcgaggcct cgacgggcag caccgagccg cccatctccg tccaggcgag
      181 cccgcaggcc aggccgggac cgggctcgga gaagaccagg tcgttctcgt ggcggcgctt
      241 cccgagcagc ttgcgtagcg acgaggcgcc gatcacgcgc ttgaactctg cgaggggcct
      301 ctgcccgccg gcgtaacccg cctcgacggc ccgctccgca gtctttctga cgactctggc
      361 tatctcccgc tcgagggagc gcaccccgga ttccatcgtg taatggcgga tgatctcgag
      421 caccgcgtcg tcgcgtatcc tcgtcgacga gccttcgagg ccgttctccc tgatctgcct
      481 cgggacgatg aagtgcctgg ctatctcgag cttctcgtac tcgctatagc cgggaatctc
      541 tatcagctcc atacggtcga gcagggcgta gggtatcgag tggacgctgt tggcggtggt
      601 gacgaacatg acccccgaca ggtcgtacgg cacctcgagg tagtggtcga caaagctggc
      661 gttctgctcc gggtcgagta cctcgagcag cgcgctcgcc gggtcgcccc ggaagtcgct
      721 cgacatcttg tcgatctcgt cgaggaggaa gaccgggttc gacgagccgg ccttgcgcat
      781 gctctggagt atcttaccgg gtagcgcgcc cacgtaggtc ttacggtggc cgcgtatctc
      841 ggcctcgtcg cgcacgccgc cgaggctcat gcgcacgaag tcgcggccga gcgctcttgc
      901 gacggatctg ccgaggctag tcttgccggt gcccggcggg ccgacgaggc agagtatcgg
      961 gcccttgagc gaatcgttga gctgcctgac ggctatgaac tcgaggatgc gctccttggc
     1021 cttcctcatg ccgtaatgat cctcgtcgag cgcggccttg gccgccgcca ggtccttgga
     1081 gtcagccttg cgcgccgacc agggcaggtc ggccagccac tcgcagtagc cgcggatgac
     1141 gcccgcctcg ggcgacaggg gctgtagttt agctagcctg gccagctctt tcttggcctt
     1201 gtccagaact tcctgaggtg gcgccttcga ctcgattcgg cgggtcagtt cggccaggtc
     1261 cgactcatcg tcgtcgcgcc ccagttcctt gtggatttcc ttgagctgtt cctggaggaa
     1321 gtagtccttt tggctctttt ccatcctggc gcggaccttc tgggagatgc gcttctggat
     1381 cgacaagagc tctatgtcct gggaaagggc cttggcggta gcctcgaggc gctcgcgaag
     1441 cggtaccacg gccaggagct cttgcttccg ctccagcttg agccccatgg cgttggctat
     1501 cgttccgcat agcttatggg gagactcggc gcgctgtacg gccagcatgg tctcgttcgg
     1561 tatcttcttg acgaggtcgg cgtaggtcga gaagtcgcgg cgtaggatct ggacgagggc
     1621 ttcgagctcc ttacgcccat cggcgccgct cggtccaccg tcgtcgccg
//
LOCUS       DAOOLN010000449         1669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_93778_length_1669_cov_2.822800, whole genome shotgun sequence.
ACCESSION   DAOOLN010000449 DAOOLN010000000
VERSION     DAOOLN010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93778_length_1669_cov_2.822800"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1477)
                     /locus_tag="PLW80_06350"
     CDS             complement(<1..1477)
                     /locus_tag="PLW80_06350"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB66160.1"
                     /translation="MAEAKKIVILGGGYGGVWAGKILEKRFRKDSGVEITLIDRKPFH
                     TLMTELHEVAGWRTEPESVQVHFKKIFGAKKIDVVLDEVVSVDFKGKKAVGKARSYDF
                     DYIIVGAGAEPEYFNIPGVKENSFSLWSFDDAMRLRHHVEHMFAKAAEETDPAVRRRL
                     LTFVVAGAGFTGMEMIGELLEYRDAMCRKHYLDRSESRVVNIEALPSILPILEEPLRV
                     KAEKYLAKMGCEVMLNASIIGAEPGKVLCKDGTVIETDTFIWTCGVKGSSFAAGLDLT
                     KGERGRGRLQVDAEMKSVDYPYAYVVGDNAFFIENGKPLPQIVEGAHQTAEVAAKNII
                     ADIEGKAERHQFKSAFHGFMVSLGGRYGVANAGGMKTSGFFAMAMKHMINMWYLLNIA
                     GVNQVWEYAKHEFLDIKERRSFIGGFASWKVRGYWPLLLRMWLGLMWVMEGVNKIGEG
                     WLTWSAGSKSGWMFSPGVKQAGIKAVEAVSAASGEWAEPATE"
BASE COUNT          317 a          508 c          487 g          357 t
ORIGIN      
        1 tctcagtcgc gggctcggcc cattcgccgg aggcggcgct gacggcttcg accgccttga
       61 tgccggcctg tttaacgccc gggctgaaca tccatccgct cttagacccg gcggaccaag
      121 tcagccagcc ctcgccgatc ttgttgacgc cctccattac ccacatgagg ccgagccaca
      181 tgcgcagaag tagcggccag tagccacgta ccttccagga ggcgaagccg ccgatgaacg
      241 agcggcgttc cttaatgtca aggaactcgt gcttggcata ttcccagacc tggttcacgc
      301 cggcgatgtt caggaggtac cacatgttga tcatgtgctt catcgccatc gcgaagaacc
      361 cgctcgtctt cataccgccg gcgttggcca cgccgtagcg gccgccgagc gataccatga
      421 agccgtggaa ggccgacttg aactggtggc gttcggcctt gccctcgatg tcggcgatga
      481 tgttcttggc tgccacctcg gcggtctggt gggcgccctc gacgatctgc gggagcggct
      541 tgccgttctc gatgaaaaag gcgttatcgc cgacgacgta cgcgtacggg tagtcgacgc
      601 ttttcatctc ggcgtcgacc tgcaggcggc cgcggccgcg ctcgcccttg gtcagatcga
      661 ggccggcagc gaaggagcta cccttgacac cgcaggtcca gatgaaggta tcggtctcga
      721 tgaccgtacc gtccttgcat agcaccttgc cgggctcggc gccgatgatc gacgcgttga
      781 gcatgacctc gcagcccatt ttggccaggt acttctccgc cttgacgcgg agcggctcct
      841 cgaggatggg caggattgac ggcagggcct cgatattgac gacgcgggac tcggagcgat
      901 ccagataatg tttgcggcac atcgcgtcac ggtattcgag cagctcgccg atcatttcca
      961 tgccggtgaa gccggcgccg gcgacgacga aggtgagcag acgcctacgg acggcgggat
     1021 cggtctcctc ggcagctttg gcgaacatgt gctcgacgtg gtggcggagg cgcatcgcgt
     1081 cgtcgaacga ccagagactg aaggagttct ccttgacgcc aggtatattg aagtactcgg
     1141 gctcggcacc tgccccgacg attatgtagt cgaaatcgta cgagcgagcc ttgccgacgg
     1201 ccttcttgcc cttgaagtcg accgatacga cctcgtcgag gaccacgtcg atcttcttgg
     1261 cgccgaatat tttcttgaaa tgcacctgga ccgactcggg ctcggtgcgc cagccagcta
     1321 cctcgtgtag ttcggtcatc aacgtgtgga acggctttct atcgatgagc gtgatctcca
     1381 cgccgctgtc cttgcggaaa cgcttctcaa gtatctttcc ggcccagacg ccgccgtacc
     1441 ctcctccgag gattactatc ttcttcgctt cggccatagt ctctccttgc cagcctagtg
     1501 taatgcggaa tggtacccgc gacgctaaaa tttatcatcg cgtgacggaa aacgactaat
     1561 atgagttatg tatacatagt tataggaaaa tgtatatgtt tggcaagccc tggctagtgt
     1621 tacgaatctt aagcagtctc ccgccactcg ccgatcaacc gaaggcata
//
LOCUS       DAOOLN010000450         1661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_94529_length_1661_cov_2.351806, whole genome shotgun sequence.
ACCESSION   DAOOLN010000450 DAOOLN010000000
VERSION     DAOOLN010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94529_length_1661_cov_2.351806"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PLW80_06355"
     CDS             complement(<1..502)
                     /locus_tag="PLW80_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPB66161.1"
                     /translation="MSQTISLTTIILCGGAGTRLWPLSRKALPKQFAALLPGGSLFQG
                     AVRRALALGSRVMVAANEGQSSIAAAQLASLGVLYYGSMVEPVGRNTAPAIALACMAL
                     EPGEVVLVAPSDHLIRGLPEYLAAATRGTELAKQGRIVTFGIAPAYAETGYGYIEADG
                     EAVRSFK"
     gene            complement(499..894)
                     /locus_tag="PLW80_06360"
     CDS             complement(499..894)
                     /locus_tag="PLW80_06360"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HPB66162.1"
                     /translation="MLERLAKLDQNVSSLEMLRAENAPEAFGSSITLQWALRYGLIES
                     IQIVIDTACEVCSKNNLGVPSSYGDCVRLLARARYLSSDCAEELVKVIGLRNLLIHEY
                     TAIDTGRLYGFLAQLDIFRRYAKELRPYL"
     gene            complement(887..1336)
                     /locus_tag="PLW80_06365"
     CDS             complement(887..1336)
                     /locus_tag="PLW80_06365"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPB66163.1"
                     /translation="MDRTKRSVDAGLLEALRDALSPVASIRLVVLFGSRARGEARPDS
                     DVDVAVSFGSTPSLLDFGDVVSRCEAATGLNVDVVELDGLPSSKPALAYEIARDGTVV
                     LDRDDGFWRYRARAFSLYLDQRDFLERQRALFSDRLDEGNLGMAKHA"
     gene            complement(1367..>1661)
                     /locus_tag="PLW80_06370"
     CDS             complement(1367..>1661)
                     /locus_tag="PLW80_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66164.1"
                     /translation="GLYRDRIESYVMARKRAGARRLVLAGVSDLDFLLEYACERHGIL
                     FVKAVDPDKAARLGQDPDTVVLRAEGTPDGSPSGTCPATEESLSRVIVGAAGA"
BASE COUNT          303 a          555 c          522 g          281 t
ORIGIN      
        1 ccttgaacga gcgcacggcc tcgccgtcgg cctcgatgta gccgtagccg gtctccgcgt
       61 aggcgggcgc tatgccgaag gtgacgatac gcccctgttt agcgagctcc gtcccccggg
      121 tagctgccgc caggtactcg ggcaggcctc ggatcaggtg gtcgctcggc gccacgagca
      181 cgacctcgcc cggctcgagg gccatgcagg cgagggctat cgccggcgcc gtattacggc
      241 cgaccggctc gaccatcgag ccgtagtaca gcacgccgag ggaggcgagc tgcgccgcgg
      301 cgatcgagct ctgtccctcg ttcgcggcga ccatcacgcg ggagcccaag gctagagccc
      361 tccgtacggc tccctggaaa aggctgccgc cgggcaggag cgcggcgaac tgcttgggca
      421 gcgccttgcg ggacagcggc cacagcctgg taccggcgcc gccgcagagg attatcgtcg
      481 tcaacgagat cgtctggctc ataggtatgg cctaagctcc ttcgcgtatc gcctgaaaat
      541 atctagttgg gccaggaagc cgtagaggcg ccccgtatcg atagccgtat actcatggat
      601 aaggagattc ctgaggccga tgaccttgac cagctcttcg gcgcagtcgg agcttaggta
      661 tcgcgctcgc gcgagcaatc ggacgcagtc gccgtagcta gacggtacgc ccaggttgtt
      721 cttagagcaa acctcgcagg ccgtatcgat gacgatctgg atcgactcta tcaagccata
      781 gcgaagcgcc cattgtaggg ttatcgagga accgaacgct tccggggcgt tctctgctcg
      841 tagcatctcg aggcttgaaa cgttctggtc tagcttcgcc aatctctcaa gcatgcttcg
      901 ccatccctaa gttcccttcg tcaagacgat ccgagaaaag ggcccgttgc cgttccaaga
      961 agtctcgctg gtccagatag aggctgaacg ccctcgccct atagcgccag aaaccgtcgt
     1021 cgcggtcgag gacgacggtt ccgtcccggg ctatctcgta ggccaaggcc ggtttcgacg
     1081 aggggaggcc gtcgagctcg acgacgtcta cgttcagccc cgtggcggct tcacatctcg
     1141 acacgacgtc cccgaagtcc aggagcgacg gcgtcgatcc gaacgatacg gccacgtcga
     1201 cgtcgctatc gggtcgcgct tcgccacggg cccgcgaacc gaacagcacg acgagccgta
     1261 tcgacgccac gggagagaga gcgtcgcgaa gggcttcgag caaccctgca tctaccgaac
     1321 gcttagtcct atccacgata gcgcccccgt acccgtccac gaatccttac gcgccagccg
     1381 cgccgacgat cacgcgggac aggctctcct ccgtagccgg gcaagtaccc gacggcgatc
     1441 cgtcaggcgt cccttcggcc cggagcacga cggtatcggg atcctggccg aggcgggccg
     1501 ccttgtccgg gtcgaccgcc ttgacgaaca ggatgccgtg ccgctcgcag gcgtactcca
     1561 gcaggaagtc gaggtccgat accccggcca gcacgagcct gcgcgctccg gcgcgcttgc
     1621 gggccatcac gtacgactcg atgcggtctc ggtagagccc g
//
LOCUS       DAOOLN010000451         1655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_95071_length_1655_cov_3.525000, whole genome shotgun sequence.
ACCESSION   DAOOLN010000451 DAOOLN010000000
VERSION     DAOOLN010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95071_length_1655_cov_3.525000"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PLW80_06375"
     CDS             complement(<1..772)
                     /locus_tag="PLW80_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB66165.1"
                     /translation="MSLRRSFGDWIEASADRGSIRLEGEAPSYDDKVRAGWLAARYGF
                     KGVVNDARSPEEAAERLTPRGAAAPSLDGSYYDVAIVGGGVIGCAIARELSRWDLSIV
                     LLEKESDVAAHTSSRNDGMIHDGFAAKPGSKKAAYNVRGNRLWEPLCIELGLDFKRPG
                     SLVLFRTAAAAALYPAMAARARRNGVDGWEYWSRARVAAEEPNVTPDQHGAFFLPSAG
                     ILSPYRATVALAESAAMNGARVSLGACVLGMELEGGSIV"
     gene            947..>1655
                     /locus_tag="PLW80_06380"
     CDS             947..>1655
                     /locus_tag="PLW80_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66166.1"
                     /translation="MVKASGFIISFIALAAAYSCASAPSSGSLDGDTGTAGGMAGGAL
                     VAGLLLDSKEARRVPARKPRAMLAIASPRVLGKAIGMGVLEEKGYPTWRLSRSEALFD
                     VVKVRIGFSARLPEGGVERQSGMLSIPAPSGGGRRELSWIVFLKGSEHHRDKVPSRRG
                     ANESALMDAAAALGYAVWAPDYAGMGDAEGAQEYCVPESMAASALDGLAAARAYLADR
                     GDEYAESGRLYIIGYSQG"
BASE COUNT          265 a          569 c          545 g          276 t
ORIGIN      
        1 cgacgatgga gccgccctcg agctccatgc cgagcacgca ggcgccgagc gacacgcgcg
       61 cgccgttcat cgcggcgctc tccgcgagcg ccaccgtcgc ccgatacggc gacaggatgc
      121 cggcgctcgg caggaagaag gcgccgtgct ggtcgggcgt cacgttcggc tcctcggccg
      181 cgacccgcgc gcggctccag tactcccagc cgtcgacgcc gttgcggcga gccctggcgg
      241 ccatcgccgg gtacagggcg gcggccgcgg ccgtgcgaaa cagcacgagc gatcctggcc
      301 gcttgaagtc caggccgagt tcgatgcaca ggggctccca gagccggttg ccccggacgt
      361 tgtacgcggc cttcttcgat ccgggcttcg ccgcgaagcc gtcgtggatc atgccgtcgt
      421 tacggctcga ggtatgggcc gccacgtcgc tctccttctc gaggaggacg atggatagat
      481 cccagcgcga cagttccctg gctatcgcgc agccgatcac gccgccgccg acgatggcca
      541 cgtcgtaata cgagccgtcg agcgatggag cggcggcgcc gcgcggtgtc agccgctcgg
      601 cggcttcttc cggggagcgg gcatcgttca cgacgccctt gaacccgtag cgggccgcca
      661 gccaaccggc tcgtacctta tcatcgtagc tcggcgcctc accctcgagc cgtatcgagc
      721 cgcgatcggc tgacgcctcg atccagtcac cgaaactcct ccgaagcgac aagcgcgctc
      781 gctccagccg cctcgagtcg gggtctgcgg tacagactct atcggtacac cttcttggta
      841 ttaccagcat accagtctta taatccatga ataccggatc gtcaagttat ttttacccgg
      901 tgcagcgcta tggacgatcg tcgtcgtcgc gctatatgtt catccaatgg taaaagcatc
      961 cggcttcatc atctcgttca tcgccctagc cgccgcctac tcctgcgcga gcgctccatc
     1021 atcggggtcc ttggacggcg acaccggaac cgctggcggc atggccggcg gcgccctcgt
     1081 cgcgggcctc ttgctcgact cgaaggaagc caggcgcgtg ccggccagaa agccgcgcgc
     1141 catgctggcc atagcctcgc cgcgagtgct cggtaaggcc atcggcatgg gcgtcctcga
     1201 ggagaagggc tatccgacct ggaggctgtc gcgtagcgag gctctgttcg acgtggtcaa
     1261 ggtcagaata ggcttctcgg ccaggcttcc ggagggagga gtcgagcgcc agagcggcat
     1321 gctgtcgatt ccggctccgt ccggcggcgg aaggcgcgag ctgagctgga tcgtcttcct
     1381 gaaaggctcc gagcaccatc gcgacaaggt tccgtcacgt aggggcgcca acgagtccgc
     1441 cctcatggac gctgccgccg ccctcggcta cgcggtatgg gctcccgact acgcgggtat
     1501 gggcgacgcc gagggagccc aggaatactg cgtgcccgag tcgatggccg cctccgccct
     1561 cgacgggctg gccgcggcca gggcctacct ggccgacaga ggcgacgagt acgccgagtc
     1621 gggcaggctg tatatcatcg gctactcgca gggcg
//
LOCUS       DAOOLN010000452         1655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_95104_length_1655_cov_2.419375, whole genome shotgun sequence.
ACCESSION   DAOOLN010000452 DAOOLN010000000
VERSION     DAOOLN010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95104_length_1655_cov_2.419375"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLW80_06385"
     CDS             <1..334
                     /locus_tag="PLW80_06385"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HPB66167.1"
                     /translation="GDRARELTLALPGRFRAEAASGFEGTVGLALSDCAGGFSLTVAG
                     RTISVIETSVPVTVPDTAVLCLRVSSSTWASILEGRSRIETAYLRGKLRVDGEIALAL
                     RLRDMLGL"
     gene            406..>1655
                     /locus_tag="PLW80_06390"
     CDS             406..>1655
                     /locus_tag="PLW80_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPB66168.1"
                     /translation="MTTEIRNLGYYAGGRQEISATTTYMDCFDPSTGEVIARAPQCLP
                     EEVEEAVAAAAAAYPAWSSTPVTKRVQVLFRLKTLMDARLDELAELTAREHGKVWDEA
                     MGDVLKAIEVVEYACGAPSLMQAPALMDCSPGYDTAQYRESLGVFAGIVPWNFPAMIP
                     MGWMAPLCVATGNTMVIKAASYTPQTAMRIMELWAEAGLPPGVLNLVTCSRAEAELLL
                     RHPAIVGVSFVGSTSVGRHIYETAAAAGKRVQCLTEAKNHALVLSDAAVERTAAGIVN
                     SACGCAGERCMALPVVVAEEAIADELVASLRRRMEGLRIGPAYDKASRLGPLVNAEHR
                     RSVEAWIRKGVDEGAELVLDGRGVSVPGYEGGFYLGPTLFDRVAPGMSIGEREIFGPV
                     LCVKRVRDFDEGLAVMNAGEFANGS"
BASE COUNT          266 a          532 c          582 g          275 t
ORIGIN      
        1 cggcgaccgg gcccgcgagc tgaccctggc cctgcccggc cgattcaggg ccgaggcggc
       61 aagcgggttt gaaggcactg tcggcctcgc cctgagcgat tgcgcgggcg gcttctcgct
      121 cacggtcgcc ggtagaacca taagcgttat cgagacctct gtccccgtta cggttcccga
      181 taccgccgtc ctctgcctac gggtctcgag ctccacctgg gcctccatcc tcgaggggcg
      241 gtcgcgcatc gagacggcct acctgcgtgg caagctgcgc gtcgacgggg agattgccct
      301 ggccctgagg ctcagggaca tgctgggttt ataaagccgc ttcctgagga cagagcttcg
      361 cttccgggga cagagcttcg cttcaacgac aaggaaggag aagatatgac tacagaaatt
      421 agaaacctgg gatactacgc gggtggtcgg caagagatat ccgcgacgac gacatacatg
      481 gactgcttcg acccttcaac gggcgaggtc atagccaggg cgccgcagtg cctgcccgaa
      541 gaggtggagg aggccgtcgc cgcggccgcg gccgcctatc ccgcctggtc gtcgaccccg
      601 gtgacgaagc gcgtccaggt cctgttcagg ctcaagaccc tgatggacgc ccgcctggac
      661 gagctcgccg agctgacggc ccgcgagcac ggcaaggtct gggacgaggc gatgggcgac
      721 gtgctcaagg cgatcgaggt ggtcgagtac gcgtgcggcg cgccgtcgct gatgcaggcg
      781 cccgcgctga tggactgttc gcccggctac gacaccgccc agtaccgcga gagcctcggc
      841 gtgttcgccg gcatcgtgcc gtggaatttc ccggcgatga taccgatggg ctggatggcg
      901 cccctctgcg tggccacggg caatacgatg gtcatcaagg cggccagcta cacgccccag
      961 acggcgatgc gtatcatgga gctctgggca gaggccggct tgcctccggg agtcctcaat
     1021 ctggtcacct gctcgagggc cgaggcggag ctactgttac ggcacccggc gatagtcggc
     1081 gtcagcttcg tcggctcgac gagtgtcggg cgccatattt atgagacggc cgccgctgcg
     1141 ggcaaacggg tgcagtgcct gaccgaggcc aagaaccacg ccctggtcct atccgacgcg
     1201 gccgtcgagc ggacggccgc cggcatcgtc aactcggcct gcggctgcgc cggcgagcgt
     1261 tgcatggcgc ttccggtcgt cgtggccgag gaggcgatag ccgacgagct cgtggcatca
     1321 ttgcggcgcc ggatggaagg gctacggatc gggccggcct acgacaaggc ctcgaggctc
     1381 gggcccctcg tcaacgcgga gcaccgacgc tcggtcgagg cctggatccg gaagggcgtc
     1441 gatgagggcg ccgagctggt cctcgacggc cgcggcgtct ccgtgcctgg ttacgagggc
     1501 ggtttctacc tgggcccgac tctcttcgac cgtgttgccc ctggcatgtc gatcggcgag
     1561 cgggagattt tcgggccggt cctctgcgtc aagcgcgtgc gggacttcga cgagggcctc
     1621 gccgtgatga acgccggcga attcgcgaac ggctc
//
LOCUS       DAOOLN010000453         1654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_95154_length_1654_cov_4.462789, whole genome shotgun sequence.
ACCESSION   DAOOLN010000453 DAOOLN010000000
VERSION     DAOOLN010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95154_length_1654_cov_4.462789"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..1220)
                     /locus_tag="PLW80_06395"
     CDS             complement(87..1220)
                     /locus_tag="PLW80_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HPB66169.1"
                     /translation="MATGTVPRTAARVFRSVWRKPGISRIELAGALGLDKSTVTNQVN
                     HLIDLGLILETEEGESSVKGGRRPIQLVINRKFGRVIGIEVQLGMYVAVVVDLSGEIL
                     GERRGRLDITEGNFAENVLRIVAETTAEFGCADDLLGVGVGIGGLVDSKKGRIRYSVP
                     IGITRPIDFGKAVASRLTVPCSVENDANCCAWGELAFNRGDEPRDFLFALVEYRRPLA
                     LSLRGGVGVGFGIVLGGKVHAGVHGNAGEFRSVFCDGPGELQFSLREDELSRLDTDVD
                     ALGRTADELARNMAMLVNTMDFDRVYIGGDIEALNVDLPGALRRRLEENWMYPAPKDV
                     DIRYSSLGGKAVAYGAAGMIFDRLVTERLLPGLGADPPSGHTM"
     gene            1350..>1654
                     /locus_tag="PLW80_06400"
     CDS             1350..>1654
                     /locus_tag="PLW80_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HPB66170.1"
                     /translation="MVRIDLAGTWRLERLSDHSVRPMAAPGDIMSALVASGEIPDPYY
                     DREELGLQWIGREDWRLYRSFDATERLLKRRRVRLEIDSVDTIAEISLNGRIVGASD"
BASE COUNT          308 a          562 c          494 g          290 t
ORIGIN      
        1 cgatcattct cttcattcta gcctccttga taacgggccc gcggcccgcc tcagaaaccg
       61 ttctatatcg acccctacgg ggacgttcac atggtatgcc cgctcggcgg atcggcgccg
      121 agccccggga gcagccgctc cgttaccagt cggtcgaaga tcatgccggc cgcgccgtac
      181 gcgacggcct taccgccgag actcgagtag cggatgtcca cgtccttcgg ggccggatac
      241 atccagttct cctcgagccg acgcctgagc gcgccgggca gatccacgtt cagagcctcg
      301 atgtcgccgc ctatgtagac gcggtcgaag tccatcgtgt tgacgagcat cgccatgttc
      361 ctggccagct cgtcggcggt cctcccgagg gcgtcgacgt cggtatcgag cctggacagc
      421 tcgtcctcgc ggagcgagaa ctgcagctcg cccggaccgt cgcagaacac gctgcggaac
      481 tcgccggcgt taccgtgcac gcccgcgtgc accttgccgc cgagcacgat gccgaagccc
      541 acgccgacgc cgcctcgcag gctcagggcc agaggcctcc gatactcgac gagcgcgaac
      601 aggaaatcgc gcggctcgtc gccgcggttg aacgccagct cgccccaggc gcagcagttc
      661 gcgtcgttct cgacggaaca cggcaccgtg agcctcgagg cgaccgcctt gccgaaatct
      721 atgggcctgg tgatgccaat cggaacggaa tagcggattc ggcccttctt cgagtcgacc
      781 aggccgccga tgcctacgcc gacgccgagc aggtcgtccg cgcagccgaa ttcggcggtt
      841 gtctcggcta cgatgcgcaa cacgttctcg gcgaaattgc cctccgtgat gtcgaggcgg
      901 cctcgccttt ccccgaggat ctcgcccgac aggtcgacca cgacggccac gtacatgccg
      961 agctgaacct ctatgccgat cacgcggccg aacttccggt tgatcacgag ctggatgggc
     1021 cgacgaccgc ccttgaccga cgactcgccc tcctcggtct cgaggatcag gccgaggtct
     1081 atgaggtggt tgacctggtt ggtgacggtg gatttatcga ggcccagcgc gccggccagc
     1141 tcgatacggc tgatgcctgg cttacgccat acggacctga aaaccctcgc ggccgtcctc
     1201 ggaaccgttc cagtcgccac cgctcctcct ccagcgcctc acaatataag ttggttgatc
     1261 taaccaacca atcatgctat accatgagtt cagataaccg tcaacatcgt ttttattgca
     1321 tcgaccaaaa accctgaacg gaggcgacga tggtacgaat cgatctggcc ggaacctgga
     1381 ggctcgagcg cctgtccgat cacagcgtac ggccgatggc ggctcccggc gacatcatgt
     1441 cggccctcgt cgcctcgggg gagatacccg acccgtacta cgaccgcgag gagctcggcc
     1501 tccaatggat aggacgcgag gattggcggc tgtaccgaag cttcgacgcg acggagaggc
     1561 tccttaagcg tcgacgcgtc cgcctcgaga tagacagcgt cgatacgata gccgaaatca
     1621 gcttgaacgg ccgtatcgtc ggcgcgagcg acaa
//
LOCUS       DAOOLN010000454         1652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_95349_length_1652_cov_3.123982, whole genome shotgun sequence.
ACCESSION   DAOOLN010000454 DAOOLN010000000
VERSION     DAOOLN010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95349_length_1652_cov_3.123982"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="PLW80_06405"
     CDS             <1..828
                     /locus_tag="PLW80_06405"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPB66171.1"
                     /translation="SLQSQAAALESTAAAAEARGVRHGVTLMLDLGDLREGWFDRQEL
                     VAAALRVERGLPSLRLRGIGTNLGCYGAVLPTRENLGRLLEAAREVEDAIGRRLDVVS
                     GGATTSLPLVARGRMPAGVNELRIGEAILCGKDLPLFHRVELPGVRGDTITLRAELLE
                     KHVKPSYPIGARLRDAFGACPEYEDRGTRTRLLLGVGKRDIGNHELLRPRDGRIHIVG
                     SSSDYLICEADEPCDDLRYGSILEFDMLYGAMLFLCDANPYLAVDYRAAEGSNPVEP"
     gene            complement(830..1312)
                     /locus_tag="PLW80_06410"
     CDS             complement(830..1312)
                     /locus_tag="PLW80_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HPB66172.1"
                     /translation="MSEPCPCGSGRDYAECCGPIIAGKAKAPTAEALMRSRYTAYAKG
                     AVDHILSSCVQDDGIDAEATRDWSESADWKGLTIVRTEKGGPGDDEGVVEFVARYEMD
                     GLKEEHHETARFVREGGAWLYDSGEVKTATVVRATPKVGRNEKCPCGSGKKYKQCCGK
                     "
     gene            1449..>1652
                     /locus_tag="PLW80_06415"
     CDS             1449..>1652
                     /locus_tag="PLW80_06415"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HPB66173.1"
                     /translation="MQDALMRAWLASRSACSGGTVASYIPALAGADGSALGLAAVDAD
                     GHVYEAGDSRSRFTIQSISKVLAL"
BASE COUNT          245 a          562 c          568 g          277 t
ORIGIN      
        1 agccttcagt cgcaggctgc cgcgctcgag tcgacggctg ccgcggccga ggcccggggc
       61 gtcaggcacg gcgtcaccct gatgctcgac ctcggcgacc tgcgcgaggg ctggttcgat
      121 aggcaggagc tcgtcgcggc ggcgctgagg gtcgagcgcg ggttaccgtc cctgcgcctg
      181 cgcggcatcg ggaccaacct cggctgctac ggggcggttc tgccaacccg ggagaatctc
      241 ggccggcttc tcgaggcggc gcgcgaggtg gaagacgcga taggccggag gttggacgtg
      301 gtctcgggcg gggcaacgac gagcctgccc ctcgtcgccc gcggccgtat gcccgccggc
      361 gtcaacgagc tgcggatagg cgaggcgata ctctgcggca aggacctgcc cctgttccac
      421 cgcgtcgagc tgcccggcgt acgcggcgac acgataaccc tgcgcgccga gctgctcgag
      481 aagcacgtaa agccgagcta tccgataggg gcgcgcctgc gggacgcgtt cggggcctgt
      541 ccggaatacg aggaccgggg tacgcggacc aggctgctcc tcggcgtcgg caagcgggac
      601 atcgggaacc acgagctcct gaggcctcgc gacggccgaa tccatatcgt cggctcgtcg
      661 agcgactacc tgatctgcga ggcggacgag ccgtgcgacg acctgcgcta cggctctatc
      721 ctggaattcg acatgctgta cggcgccatg ctgttcctct gcgacgccaa cccgtatctg
      781 gccgtcgatt accgggcggc cgaggggagc aaccccgtgg agccctaggc tacttgccgc
      841 agcactgctt atatttcttg cccgagccgc aggggcactt ctcgttgcgg ccgaccttcg
      901 gggtggccct gaccacggtg gcggtcttga cctcgccgga gtcgtagagc cacgcgccgc
      961 cttcgcggac gaaacgcgcc gtctcgtggt gctcttcctt gaggccgtcc atctcgtatc
     1021 gggcgacgaa ttcgacgacg ccctcatcgt ctccggggcc gcctttctcg gtcctgacta
     1081 tcgtcaggcc cttccagtcg gcgctctcgc tccagtcccg cgtcgcctcg gcgtcgatgc
     1141 cgtcgtcctg cacgcacgat gacagaatgt gatcgacggc gcccttggcg taggccgtat
     1201 agcgagatcg catcagcgcc tcggcggtcg gggccttggc cttgccggcg atgatggggc
     1261 cgcagcattc ggcgtaatcg cggcctgagc cgcagggaca gggttcgctc atcggtatcc
     1321 tccggaggct acgttgtagc gtgaaacggc cgtcccaggc aagagaagga catggtctcg
     1381 caccggcggc cgcgcatggg cggccgcaca ggattgcagc ggatacggcc gccgtgtaca
     1441 atgccgccat gcaagacgca ctcatgcgag cctggctggc gtcgcggtcg gcatgctccg
     1501 gcggcaccgt cgcctcgtat atacccgctc tagccggagc cgacggctct gccctcgggc
     1561 tggccgccgt ggatgccgac ggccatgtct acgaggccgg cgactcgagg agccgcttca
     1621 cgatccagag catctccaag gtcctggcgc tc
//
LOCUS       DAOOLN010000455         1652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_95360_length_1652_cov_2.772699, whole genome shotgun sequence.
ACCESSION   DAOOLN010000455 DAOOLN010000000
VERSION     DAOOLN010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95360_length_1652_cov_2.772699"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /locus_tag="PLW80_06420"
     CDS             <1..1381
                     /locus_tag="PLW80_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPB66174.1"
                     /translation="ESYLRSFAGGFVVVSHDRSFLDSTVNEVYELWNGRLTRYPGSYT
                     AYERKRAQELETLFKAWEAQQEEIARLEDFIRRFRYQATKAAQVQSRIKQLDKVVPIV
                     IPEGMKRVHFSFPPAPHSGRIALTLDGVRKSYGPKRVVDELSLTVDAGSKIAFVGRNG
                     AGKSTLMRLVAGVDGDYEGSIRLGTGITVGYFSQDVADALDESLTVEEEASSACPTAL
                     LPGIRNLLGAFLFRGDDVEKRVSVLSGGERSRLALLKMLLHPANLLVLDEPTNHLDLD
                     SKDVLLDALKRFDGTVLFVSHDRFFIEGLADRVLELSCGSSPRLFYGDYAYYLTKKAE
                     ERAGEVDEPARATRSGASFAPAEDGARERNGRPLDAKASRELDKRRKAERNRLRKRED
                     EILARMEAVGKEKADALAAMALPENYSDGDRMRRLQARSQALDGEAAALSAEWEVVAT
                     ELEGYADVD"
     gene            complement(1394..>1652)
                     /locus_tag="PLW80_06425"
     CDS             complement(1394..>1652)
                     /locus_tag="PLW80_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66175.1"
                     /translation="LEATGATFAEVESDSALATSYRRKGFIAAVVVHGFYDFFLFAGP
                     PLAVGAILTLFVAVRALRRLVRRAKELDDAAASTRRALGLS"
BASE COUNT          290 a          554 c          558 g          250 t
ORIGIN      
        1 cgagtcgtac ctgcgctcgt tcgccggcgg cttcgtcgtc gtgtcccacg accgctcctt
       61 cctcgactcg accgtgaacg aagtctacga gctatggaac ggcaggctga cccgttatcc
      121 cggctcctac acggcctacg agcgtaagcg ggcccaggag ctcgagaccc tgttcaaggc
      181 ctgggaggcc caacaggagg agatagccag gctcgaggac ttcatccggc gcttccgtta
      241 ccaggcgacc aaggccgccc aggtccagtc caggatcaag caactggaca aggtcgtacc
      301 catcgtcatc ccggaaggca tgaaacgcgt ccatttcagc ttcccgccgg ccccccattc
      361 ggggcgcata gcgctgacgc tcgacggtgt acgcaagtcc tatgggccga agcgcgtcgt
      421 cgacgagctg tcgctgaccg tcgacgcggg ctcgaagata gccttcgtcg gccgcaacgg
      481 cgcgggcaag tcgaccctga tgcggctcgt cgccggggtc gacggcgact acgaaggctc
      541 gatacggctc ggaacgggca ttacggtggg ctatttctcg caggacgtgg ccgatgcgct
      601 cgacgaatcg ctgaccgtcg aggaggaggc gtcgtcggcc tgcccgacgg ccctcctgcc
      661 gggcatacgc aacctgcttg gcgcgttcct gttccgtggc gacgacgtgg agaagcgcgt
      721 ctcggtactg tccggcggcg agcgctcgcg gctggccctg ctcaagatgc tcctccatcc
      781 ggccaacctg ctcgtgctcg acgagccgac caaccacctc gacctcgact cgaaggacgt
      841 gctcctcgac gccctcaagc gtttcgacgg cacggtgctg ttcgtgtccc acgaccgctt
      901 cttcatcgaa ggcctggccg atcgcgtgct cgagctgtcc tgtggcagct cgccgcggct
      961 gttctacggc gactacgcct attacctgac aaagaaggcc gaggagcggg ccggcgaggt
     1021 cgacgagccg gccagggcca ccaggtccgg cgcctcgttc gctccggccg aggatggcgc
     1081 acgcgagcgg aacgggcgtc cgctcgacgc caaggcttcc cgggagctgg ataagcggcg
     1141 caaggccgag cgcaaccgcc tgcgcaagcg cgaggacgag atactggcgc gtatggaggc
     1201 cgtcgggaag gagaaggccg acgcgctggc agcgatggcc ctgccggaga attattcgga
     1261 cggggatcgc atgcggcgcc tccaagccag gtcgcaggcc ctcgacggcg aggccgccgc
     1321 gctgtccgcc gaatgggagg tcgtggctac agagctcgag ggctacgcgg acgtggatta
     1381 agccgcggcg ggatcaggaa aggcccaggg ctcgccgcgt cgaggcggcc gcgtcgtcga
     1441 gttccttggc cctgcgtact aagcgcctga gcgcccgcac agccacgaac agcgtcagga
     1501 tggcgccgac ggccagcggc gggccggcga acaggaagaa gtcatagaag ccgtgtacga
     1561 ccaccgccgc gataaaaccc ttgcgccggt aggacgtggc cagcgcgcta tcggactcga
     1621 cctcggcaaa cgtcgcgccg gtagcctcaa gc
//
LOCUS       DAOOLN010000456         1651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_95446_length_1651_cov_2.759398, whole genome shotgun sequence.
ACCESSION   DAOOLN010000456 DAOOLN010000000
VERSION     DAOOLN010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95446_length_1651_cov_2.759398"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /gene="rpsQ"
                     /locus_tag="PLW80_06430"
     CDS             <1..197
                     /gene="rpsQ"
                     /locus_tag="PLW80_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPB66176.1"
                     /translation="TIVVEIMMRKLHPLYKKYLTRSKKVKAHDEKNDAKVGDTVRVVE
                     TRPLSRDKRWRLAEIVERAK"
     gene            216..584
                     /gene="rplN"
                     /locus_tag="PLW80_06435"
     CDS             216..584
                     /gene="rplN"
                     /locus_tag="PLW80_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPB66177.1"
                     /translation="MIQVESKLNVADNSGAKTVQCIKVLGGSKRRYASIGDIIVVAVK
                     AALPNSAIKKGTIEKAVIVRVHKEYRRPDGTYIRFDDNACVIIDANKNPKGKRIFGPV
                     ARELRDKDYMKIVSLAPEVL"
     gene            595..918
                     /gene="rplX"
                     /locus_tag="PLW80_06440"
     CDS             595..918
                     /gene="rplX"
                     /locus_tag="PLW80_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPB66178.1"
                     /translation="MNDVMIKTKLKKDDLVQVISGRERGKQGKIVKIDRDKGRAIVQG
                     VNMVKKAMKKKNQNDRGGIIEIEAPVRLCNMMIVCKKCGPTRVGFKLEGDAKKRVCRK
                     CGEAL"
     gene            918..1487
                     /gene="rplE"
                     /locus_tag="PLW80_06445"
     CDS             918..1487
                     /gene="rplE"
                     /locus_tag="PLW80_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPB66179.1"
                     /translation="MAEKQQKNAYVPRLKKIYADKVASELKGEFGYTSTMQIPKVVKV
                     IVSMGVGEAKENKKILEAAVEDVGIITGQKAVKTKSRKSIATFKIREGQEIGCRVTLR
                     GAQMWEFLDRLLNVALPRVKDFRGVNPNAFDGHGNYSLGLTEQIIFPEIDYDKIEKVM
                     GMNIAIVTSARTDKEAKSLLGKLGMPFRK"
     gene            1499..>1651
                     /locus_tag="PLW80_06450"
     CDS             1499..>1651
                     /locus_tag="PLW80_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002557082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPB66180.1"
                     /translation="MARKAMILKALEKPKYMTRHVNRCKICGRPRGYLRKFDMCRICF
                     RKLASEG"
BASE COUNT          421 a          423 c          536 g          271 t
ORIGIN      
        1 agaccatcgt cgtcgagatc atgatgcgca agctgcaccc gctgtacaag aagtacttga
       61 cccggagcaa gaaggtaaag gcacacgacg agaagaacga cgccaaggtc ggcgataccg
      121 ttagagtcgt ggagactcgg ccgctgtcgc gcgataagcg ctggcgcctg gccgaaatag
      181 tcgagcgcgc gaagtaagcg gcggagagga ccggtatgat acaggttgaa tccaagctga
      241 acgtcgcgga caactccggc gccaagacgg tgcaatgcat caaggtgctc ggcggttcga
      301 agcggcgtta cgcatccatc ggcgatatta ttgtcgtggc cgtcaaggcg gccctgccta
      361 attccgccat caagaaggga accatagaga aggccgtcat cgtgcgcgta cacaaggagt
      421 accgcaggcc tgacgggacc tacatcaggt tcgacgataa cgcctgcgtc atcattgacg
      481 cgaacaagaa cccgaagggc aagcgcatct tcggtcccgt cgccagggag ctgcgcgaca
      541 aggactacat gaagatcgtg tcgctggccc ccgaggtcct ctaaggagcg ggcgatgaat
      601 gacgtcatga taaagacgaa gctgaagaag gacgatctcg tgcaggtcat ctccggcagg
      661 gagcgcggca agcagggaaa gatcgtcaag atcgatcgcg acaagggaag ggcgatcgtt
      721 cagggcgtca acatggtcaa gaaggccatg aagaagaaga accagaacga tcgcggaggg
      781 atcatcgaga tcgaggcgcc cgttcgcctc tgcaatatga tgatcgtctg caagaagtgc
      841 ggacctaccc gcgttgggtt taagctcgag ggcgacgcga agaagcgcgt ctgccggaag
      901 tgcggggagg cgctctgatg gccgagaagc agcagaagaa cgcgtacgtt ccgaggctca
      961 agaagatcta cgccgataaa gtggcgtccg aactcaaggg tgaattcggc tatacctcga
     1021 cgatgcagat cccgaaagtg gtcaaggtca tcgtgtcgat gggcgtcgga gaggccaagg
     1081 agaacaaaaa gatcctcgag gccgctgtcg aggacgtcgg catcatcacc ggccagaagg
     1141 ccgttaagac gaagtcgagg aagtcgatcg cgaccttcaa gattcgagag ggccaggaga
     1201 taggctgcag ggtcacgctc cgcggggcgc agatgtggga gttccttgat aggctcctga
     1261 acgtcgctct gccgcgcgtc aaggatttcc gtggagttaa cccgaacgcc ttcgacggcc
     1321 acgggaacta ctcgctgggc cttaccgagc agatcatctt cccggagatc gattacgata
     1381 agatcgagaa ggtgatgggt atgaacatcg ccatcgttac gagcgcccgc accgacaagg
     1441 aagccaagag cctccttggc aagctgggca tgccgttcag gaagtagggg agaatcgaat
     1501 ggccaggaaa gctatgatac tgaaggcgct cgagaagccg aagtacatga ccaggcacgt
     1561 gaatcgatgc aagatatgcg gtcggccccg ggggtatctc cggaagttcg acatgtgccg
     1621 tatttgtttc cggaagctcg cgagcgaggg c
//
LOCUS       DAOOLN010000457         1650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_95528_length_1650_cov_3.286520, whole genome shotgun sequence.
ACCESSION   DAOOLN010000457 DAOOLN010000000
VERSION     DAOOLN010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95528_length_1650_cov_3.286520"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="PLW80_06455"
     CDS             <1..557
                     /locus_tag="PLW80_06455"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HPB66181.1"
                     /translation="RGYSRPIIAVTASALKGERDKCIEVGMNDVLVKPFKKSDLATTL
                     GFWAEKASEAEAPVYATAPEANPGGRESATLDFDGLVETFLGQRGKVLELLARFSAKT
                     RLQLADLEAAAAAGDAKTLREIAHSIKGAAWSLSARALGDVAMTIEKAAADGDAQAAA
                     ASLGELGERFAEFESIAKFYSERS"
     gene            554..1159
                     /locus_tag="PLW80_06460"
     CDS             554..1159
                     /locus_tag="PLW80_06460"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPB66182.1"
                     /translation="MSGRAGQGELFGPPDVSSYRIVCGIDEAGRGPLAGPVSAAAVIL
                     PDDFPLRLLDDSKALSARARDAAYTVIVERARWANEWAWPSEIDGLNILGATMLAMRR
                     AFSRLGAAPDLVVVDGNRDPGLPCTCRPMVKADAIVPAVMAASIVAKVARDRLMERYD
                     WLYPEYGYARHKGYPTAVHRAACRALGPSPVQRLSFDYSKE"
     gene            complement(1190..>1650)
                     /locus_tag="PLW80_06465"
     CDS             complement(1190..>1650)
                     /locus_tag="PLW80_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66183.1"
                     /translation="FQKSLDAMNEAAPSERSKPPRTRREAMAPRDDGREAERRLSDDE
                     RPRHYIDTGKRRDKAAAGERGRPDRDASGRAVPGRAASGRDDARRDSVRRDIERATGR
                     SSAGPNDDRRTRTTGRVLRVKKAVKAAPAVPEKKPADKRLTVKKVPKKDE"
BASE COUNT          213 a          576 c          611 g          250 t
ORIGIN      
        1 agcgcggcta ctcgaggccc atcatcgcgg tgacggcgag cgccctcaag ggcgagcgcg
       61 acaagtgcat cgaggtcggg atgaacgacg tcctggtcaa gccgttcaag aagtccgacc
      121 tggcgacgac cctcggcttc tgggcggaga aggccagcga ggccgaggct ccggtctacg
      181 cgacggcgcc ggaggcgaac ccgggcggcc gggagagcgc gacgctcgac ttcgacggcc
      241 tcgtcgagac cttcctcggc cagcgaggca aggtactcga gctgctcgcg cgcttctccg
      301 ccaagacccg gttgcagctc gccgacctgg aggccgccgc ggccgccggc gacgccaaga
      361 cgctccgcga gatagcccac tccatcaagg gcgccgcctg gagcctgtcg gccagagccc
      421 tcggcgacgt ggccatgacg atcgagaagg cggccgccga cggcgacgcc caggcggcgg
      481 ccgcctcgct cggcgagctg ggcgagcgct tcgccgaatt cgagtcgata gccaagttct
      541 actcggagcg ttcgtgagcg ggagggcggg gcagggtgag ctcttcgggc cgcccgacgt
      601 gtcgtcgtac cgcatcgtct gcggcatcga cgaggcgggg cggggcccgc tggccggtcc
      661 ggtctcggcg gccgccgtca tcctgcccga cgactttccg ctacggctac tcgacgactc
      721 caaggccctg tcggccaggg cccgcgacgc ggcctacacc gtgatcgtcg agcgggcgcg
      781 ctgggccaac gagtgggcct ggccttccga gatcgacggg ctgaacatcc tgggggcgac
      841 gatgctggcg atgcgtcggg ccttctcgcg gctcggcgcg gcgcccgacc tggtcgtcgt
      901 cgacggcaac cgggatcctg gcctgccctg cacgtgcagg ccgatggtca aggcggacgc
      961 catcgtgccg gccgtgatgg cggcgtcgat agtcgccaag gtcgcgaggg accggctgat
     1021 ggagcgctac gattggctgt atccggagta cggctacgcg cgccacaagg gctacccgac
     1081 ggccgtgcat cgggccgcct gccgagccct ggggccgtcc ccggtgcaac ggctaagctt
     1141 cgactattcc aaggagtgat cgcggggccc ggccccgcgg cgaccggggc tattcgtcct
     1201 tcttcgggac cttcttgacc gtcaggcgct tgtcggccgg cttcttctcg ggcaccgcgg
     1261 gcgccgcctt gacggccttc ttgacgcgga gcacgcgacc ggtggtgcgg gtgcggcggt
     1321 cgtcgttcgg gccggccgag gagcgtccgg tggctctctc tatatcgcgg cgcacggaat
     1381 cgcggcgagc gtcgtcgcgt ccagacgcgg cgcgtccagg cacggcgcgt ccagacgcgt
     1441 cgcggtccgg gcggccgcgc tcgcccgcgg cggccttgtc gcgcctcttc ccggtgtcga
     1501 tgtagtgcct cggccgctcg tcgtcggaca ggcggcgctc ggcctccctg ccgtcgtcgc
     1561 gcggggccat cgcctcgcgc ctggtccggg gcggctttga gcgctcggac ggagcggcct
     1621 cgttcatcgc atcgagcgac ttctggaagg
//
LOCUS       DAOOLN010000458         1650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_95543_length_1650_cov_2.924138, whole genome shotgun sequence.
ACCESSION   DAOOLN010000458 DAOOLN010000000
VERSION     DAOOLN010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95543_length_1650_cov_2.924138"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PLW80_06470"
     CDS             complement(<1..247)
                     /locus_tag="PLW80_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66184.1"
                     /translation="MATPHNAAKEGDIAPTVLLPGDPLRAKFIAETFLENPVCYNTVR
                     NMLGFTGSYKGRPVSVQGTGMGVPSLSIRFADGGGARG"
     gene            441..1280
                     /locus_tag="PLW80_06475"
     CDS             441..1280
                     /locus_tag="PLW80_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66185.1"
                     /translation="MRTGLRIVRRAVPTGLISALCLALLASCFSLPGIGGFNPLADLQ
                     SSVEARASAEVSSAVGLSGLERKVMFNVLYSQIFFIGGFGADFYPLEETQGAVWRIKS
                     VDEDGSFTAVEAERAFLKRLPNGDSWWYLAWRGDGESWEFEALMDKDQMARTIRYYNA
                     DVKRVEAAVFDTSGKAKPGSETAPPEEAPTSELALEDLSGYKKGVETIKVGAGTFRAE
                     RIEWTYYDEDEKTTYRYTWWVDPKASGGLVKYEWAKSGSKESVSGELVSLKKGYKTKF
                     ASF"
     gene            1465..>1650
                     /locus_tag="PLW80_06480"
     CDS             1465..>1650
                     /locus_tag="PLW80_06480"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HPB66186.1"
                     /translation="MSKKTIVVALGGNALIEEGTEGTIEQQFENTRKSMAAIVGLVKE
                     GHRVVLTHGNGPQAGVHL"
BASE COUNT          289 a          521 c          550 g          290 t
ORIGIN      
        1 cgccgcgggc gccgccgcca tccgcgaacc tgatcgacag cgacggcacg cccatgccgg
       61 tgccctggac cgagacgggc ctgcccttgt aactgcccgt gaagccgagc atgttgcgca
      121 ccgtgttgta gcagacgggg ttctccagga aggtctcggc tatgaacttg gcccggagcg
      181 ggtcgcccgg caggagcacg gtcggggcta tgtcgccctc cttggctgcg ttatggggtg
      241 tggccatggt ctagtctcct caggttcgcg gatggcggcg gcgcccgcgg cgcgcctcgt
      301 acgaaggtac ctcatggccg gccctttcgt ccatgagcgt tcatctgctt ttcgctggtc
      361 ttttaccaag ggcgcgttac aatatcttac tattcaaacc gatcgcgccg ccgatcggaa
      421 gagccacgga ggtttatcga atgcgtaccg gattaagaat cgttcgccgc gccgtcccga
      481 ctggactgat atcggcgctc tgcctggcgc tactcgcctc gtgcttctcg ctgcccggca
      541 taggcggctt caacccgctc gccgacctgc agtcgtccgt cgaggccagg gcctccgccg
      601 aggtctcgtc ggccgtcggc ttgagcgggc tcgagcgtaa ggtcatgttc aacgtgctgt
      661 actcgcagat cttcttcatc ggcggtttcg gcgccgactt ctacccgctc gaggagacgc
      721 agggcgccgt atggcgcatc aagagcgtcg acgaggacgg gagcttcacg gccgtcgagg
      781 cggagcgggc ctttctcaag cggctgccga acggcgattc ctggtggtac ctggcctggc
      841 gcggtgacgg ggagtcgtgg gaattcgagg cgctgatgga taaggatcag atggcccgta
      901 cgatccgcta ctacaatgcc gacgtcaagc gcgtcgaggc ggccgtcttc gataccagcg
      961 gcaaggccaa gcccggctcg gagaccgccc cgcccgagga ggcgccgacc agcgagctgg
     1021 cgctcgagga cctctccggc tacaagaagg gcgtggagac gatcaaggtc ggcgccggca
     1081 ccttccgcgc cgagcgcatc gagtggacct actacgacga ggacgagaag acgacctacc
     1141 gctacacctg gtgggtcgat cccaaggcct cgggaggcct ggtcaagtac gagtgggcca
     1201 agagcggctc caaggagtcc gtgtccggcg agctcgtctc gctcaagaaa ggctacaaga
     1261 ccaagttcgc gtcgttctaa gcctatcctg ggccgccgcg atatgcaccc cgtatacggc
     1321 gtggcggccc gcgccgaata tggattgcat aagggccctc gattgtacgc ggcgtcggcg
     1381 ttttactata ctggaccgtg cttcgccgta gggcgcgtgg cgcctgatag caacccggcg
     1441 agccgccgca gaaggaaggt tcagatgagc aagaagacga tagtcgtcgc cctcgggggc
     1501 aatgccctga tcgaggaggg taccgagggt accatcgagc agcagttcga gaatacccgc
     1561 aagagcatgg ccgcgatcgt cggcctggtc aaggaaggcc accgcgtcgt cctgacccac
     1621 ggcaacggcc cccaggccgg ggtgcacctg
//
LOCUS       DAOOLN010000459         1649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_95650_length_1649_cov_2.578419, whole genome shotgun sequence.
ACCESSION   DAOOLN010000459 DAOOLN010000000
VERSION     DAOOLN010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95650_length_1649_cov_2.578419"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /locus_tag="PLW80_06485"
     CDS             complement(<1..1320)
                     /locus_tag="PLW80_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66187.1"
                     /translation="MTVGQKASVSLLVSTVLAAGFAALAYSGLFSVIETRFFDERVRR
                     SVDASVDGLLESTDAYHESNAARFEALLGNDFVKRSFLTNQSAQDAFDRTRAFGLLSE
                     QTPGLLGVRFIDRDGKRIHFSTFPADVVRSEALRVVYRDYGAPEDAPYAALEPSDVAQ
                     RGAVAVSSDPARRAFAYRFPFVDGFDVRRGTAVFYVSYSGLLERLVKDGRIALGDDVS
                     AVGDVGILFGSPSWGGGELVSRVEAIWSSGPNAEPVTIGSAEASGPFVLFSRRGVSGL
                     VGRLVPASWFPMPDAFRWLLLSAFFVTVYLILFLILNLRQDRFAILAARIKRFQIELL
                     EEYLDRKGDLDIDRWRGELEARRGETKERIRKSAGRLAKRRAADVDALIDKSWDEIIA
                     IMTARGERPGVVDMARFEALLKEALSKGSFVIGSAGARPSSGAEAPRV"
     gene            1486..1614
                     /locus_tag="PLW80_06490"
     CDS             1486..1614
                     /locus_tag="PLW80_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66188.1"
                     /translation="MHIRDFSLKTKVLVLTQAGLAIIAAVIAVMYVRDIAKVAQAI"
BASE COUNT          323 a          531 c          532 g          263 t
ORIGIN      
        1 gacccgggga gcttcggctc cgctcgaagg ccgcgccccc gcggagccga tgacgaagga
       61 acccttgctg agcgcttcct tgagtagagc ctcgaagcgg gccatatcga cgacgccggg
      121 acgctcgccc cgggccgtca tgatagcgat gatctcgtcc caggacttgt cgatcagagc
      181 gtcgacgtcg gccgcgcgcc gcttagccag gcggccggcg ctcttgcgta tgcgttcctt
      241 cgtctcgccg cgccgagcct cgagctcgcc cctccagcgg tcgatgtcca ggtcgccctt
      301 gcgatcgagg tattcctcga gcagctcgat ctggaagcgc ttgatcctgg ccgcaagtat
      361 ggcgaaacga tcctggcgca ggttgaggat caggaatagg atcaggtaga cggtcacgaa
      421 gaaggccgac agaagtagcc atcggaaggc gtccggcatg gggaaccatg aggccgggac
      481 caaccttccc accagccccg agacgcctct cctcgagaag agcacgaacg ggccggatgc
      541 ctctgccgag ccaatggtta ccggctcggc gttcgggccg gatgaccaga tcgcctcgac
      601 gcgcgagacg agctcgccac caccccacga cggcgagccg aacaggatgc cgacgtcgcc
      661 tacggccgag acgtcgtcgc cgagggctat gcgcccgtcc ttgacgagac gctcgagcag
      721 ccccgagtac gacacgtaga agacggcggt accgcgacgc acgtcgaagc cgtcgacgaa
      781 cggaaagcgg tacgcgaacg cgcgccttgc cgggtccgac gaaacggcga cggcgccccg
      841 ctgggctacg tcggatggct ccaacgcggc gtacggcgcg tcctcgggcg ccccgtaatc
      901 gcggtagacc accctcagcg cctccgaacg gacgacgtcg gccgggaagg tgctaaagtg
      961 gatgcgcttg ccgtcccggt cgatgaagcg gacgccgagg agtcccggcg tctgctcgga
     1021 aagtaggccg aaggccctgg tacgatcgaa ggcgtcctgg gcgctctggt tggtcaggaa
     1081 gctacgcttg acgaagtcat tgccgagcag ggcctcgaag cgggcggcgt tcgactcgtg
     1141 gtatgcgtcc gtcgactcga gcagcccgtc gaccgaggca tcgaccgacc gcctcacccg
     1201 ttcgtcgaag aaccgggtct cgatgaccga gaacagcccc gagtacgcga gcgctgcgaa
     1261 gccggccgcc agcacggtcg agacgagtag gcttactgag gctttctgcc ctacggtcat
     1321 cgctgtcctt acttgctatc tattcgtttc attatcggca tacggggacg ttttataaag
     1381 ccgctcagat cgtcgcttgc cgctcggcgc tcgtgatcat atactcatcc tagacgcgag
     1441 ccaagctcgg caagcgagtg cccgcagatc gaaccaggag accagatgca tatccgagac
     1501 ttcagcctca agacgaaggt gctcgtactg acccaggccg gactggctat catagccgcg
     1561 gtcatagcgg tcatgtacgt gagggacata gccaaggtgg cccaggcaat atagtcgaaa
     1621 agagccgcgc catcacgctg acggtagag
//
LOCUS       DAOOLN010000460         1648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_95753_length_1648_cov_2.414940, whole genome shotgun sequence.
ACCESSION   DAOOLN010000460 DAOOLN010000000
VERSION     DAOOLN010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95753_length_1648_cov_2.414940"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1002..1604)
                     /locus_tag="PLW80_06495"
     CDS             complement(1002..1604)
                     /locus_tag="PLW80_06495"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HPB66189.1"
                     /translation="MLAADLARAVGIEMSECRLDSFSKRGSTFMTKRFDRQGEGRIHF
                     ASAMTLLCRKDGDDAEAGASYLELAEFIMRSGSRPDADLRELWKRIVFSIAVSNTDDH
                     LRNHGFLLDAKGWRLSPAYDINPSPDSAGLSLAIDEHDNSLDFDLALSVAPYFRLPKP
                     DALSIIKAIRGVVSTWQERAKALGIRRTEIQLMQRAFQRV"
BASE COUNT          321 a          512 c          466 g          349 t
ORIGIN      
        1 ggcaccttcg tctcgctacg cttcccgatg gctacaggcg acgcgctact gctctactcg
       61 gattgcctct cggaaggccg agacgcgatg ggagagtaat tcgggcaaga gcgtatccgc
      121 gaggccttcg ccgcggctcc ggcgacttcg gccgccgcgc tcaaggcatc gatcctcgcg
      181 agctttacga cctttattgg aacggccgac cttaccgacg acctgacctt cgtggtactg
      241 ctccgcgcct gaacctgaga cacgactaag aatcatggcg gagccccgtc gcgcaacgac
      301 gctcgacgaa cccgagcgag ggccgaaggc aatcgggcag gccgcccatt cggacaatac
      361 ctggtaaggg cgatcccacc tcgaacgcct tcagcgttct cctcccctgc gagcttgcgc
      421 actgctcacg tcgcattccg cgcgcgtcga cgcgcgggta gctagtaggg ggcgggctca
      481 ctaccccgcg ctcgacatcc tgtctctcgt ttccagcccg cttcaagctt ctctcggcgc
      541 atccatgcgc ctcgtcctcg ccttcgctcg gagactttca gttcgaaccc tcacgggggt
      601 gatcacaaac gaaaaggccg tcctttcgga ccgtgcattc cgggagacac cctccggccc
      661 cctgcggtcc tgcgcacgtt ggcgcacaga taaatgcaaa ggccgatcgg ctatgccgat
      721 cggcctttgt agctcccccg cgcgggttcg aaccacggac ccagtggtta acaggcgaac
      781 tttctattac tttgcatatc accgcctgtc actatttata atcatagcaa gtatttgctt
      841 ggttttcaac agtatgcgtt agcaagcgtt tttatgggtt ttttagattt ttgcgcccaa
      901 tttgcgccca gttatttaat gctaactata taatagaaga ggaagctcac caaacattta
      961 tatagctttc gtcctcagca aatgcatgct tcactcccgg attaaaccct ttgaaacgct
     1021 ctttgcatca actggatctc ggtcctccgg atgcccaagg ccttcgcacg ctcctgccac
     1081 gtcgagacca ccccgcgtat cgccttaatg atcgagagtg catcgggctt tgggagcctg
     1141 aagtacggcg cgaccgatag ggctaagtcg aaatccagcg agttgtcatg ctcgtcgatg
     1201 gcgagggaga ggcccgccga atcaggcgaa ggattgatgt cgtaggcggg cgagagccgc
     1261 cagcccttcg catcgaggag gaagccatga ttccgaagat ggtcgtcggt gttggagacg
     1321 gcgatggaaa agacgatgcg tttccagagc tcccgcagat cggcgtcggg ccgggatccg
     1381 gagcgcatga tgaactccgc gagctcgagg tagcttgcgc cggcctcggc gtcatcgccg
     1441 tccttgcggc agagcaaggt catggcggag gcgaaatgga tacgtccctc tccttggcga
     1501 tcgaagcgct tcgtcatgaa ggtggagcct cgcttcgaga aggagtcgag cctgcattcg
     1561 ctcatctcga tgccaaccgc gcgggccagg tcggctgcga gcatctccca ggcaccccag
     1621 tcgcgttcat cgtccttgcc cgggaact
//
LOCUS       DAOOLN010000461         1648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_95760_length_1648_cov_2.213434, whole genome shotgun sequence.
ACCESSION   DAOOLN010000461 DAOOLN010000000
VERSION     DAOOLN010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95760_length_1648_cov_2.213434"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="PLW80_06500"
     CDS             <1..1219
                     /locus_tag="PLW80_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB66190.1"
                     /translation="GDVSVLLLSENLADLNPRIVASPSTIKVEIPIPDESVRSSFLEF
                     LDSQGTLLLDRGLTPAAVGAITSGLNLVNLNRLAAESWQEDRPISMEYLKEKKREIIE
                     SEAGGLLEFLETEHDLSFVSGHDFVKKRFKHAARALKQGRLDVLPMGYLIAGPVGTGK
                     SFMVSAFAGEIGVPMVKFKNFRSKWQGVTESNLEKVLTILKAMAPVAVMIDEADAFLG
                     DRDQDGDSGTSNRIFAQLASFMGDTEYRGRIIWFLITCRPDLLPIDLKRQGRAEEHLA
                     LFYPDNDADKEALFEVLVRKLDLSIRRFPVSELFKRFKHEFSGADLEAVLIRAKLKAT
                     MDGRTFVTREDLDETMTDFVPPAYPHEIELQNLVAVVECTSKEMVPRRFQNLDRSRLV
                     KDIQELKSLLGER"
     gene            complement(1538..1648)
                     /locus_tag="PLW80_06505"
                     /pseudo
     CDS             complement(1538..1648)
                     /locus_tag="PLW80_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
BASE COUNT          282 a          593 c          520 g          253 t
ORIGIN      
        1 gggcgacgtg tcggtgctgc tcctgtccga gaacctggcc gacctcaacc cgcgaatcgt
       61 cgcgagcccg tcgacgatca aggtcgagat accgataccc gacgagtccg ttcgctcgtc
      121 gttcctcgaa ttcctcgact cgcagggcac cctgctgctc gaccgcgggc tgacgcccgc
      181 cgcggtggga gcgatcacct cgggcctcaa cctcgtcaac ctgaaccgcc tggccgccga
      241 gagctggcag gaggacaggc cgatctcgat ggagtacctc aaggagaaga agcgcgagat
      301 catcgagagc gaggccggcg gcctgctcga gttcctggag accgagcacg acctgtcctt
      361 cgtctcgggc cacgacttcg tcaagaagcg cttcaagcac gcggccaggg ccctcaagca
      421 gggccggctc gacgtactgc cgatgggcta cctgatagcc ggcccggtcg gcacgggcaa
      481 gagcttcatg gtctcggcct tcgccggaga gataggcgtg ccgatggtca agttcaagaa
      541 cttccgttcc aaatggcaag gcgttaccga gtcgaacctc gaaaaggtgc tgaccatact
      601 caaggccatg gcgccggtcg cggtgatgat agacgaggcc gacgccttcc tcggcgaccg
      661 cgaccaggac ggcgactcgg gcacctcgaa ccgcatcttc gcccagctcg ccagcttcat
      721 gggcgacacc gagtaccgcg gccgcatcat ctggttcctg atcacctgcc ggcccgacct
      781 cctgcccatc gacctgaagc gccagggccg cgccgaggag cacctcgccc tcttctaccc
      841 cgacaacgac gcggacaagg aggccctctt cgaggtgctc gtgcgcaagc tcgacctgtc
      901 catccggcgc ttccccgtct ccgagctgtt caagcgcttc aagcacgagt tctccggcgc
      961 cgacctcgag gcggtgctga tacgggccaa gctcaaggcg acgatggacg gcaggacctt
     1021 cgtaacccgc gaggacctcg acgagacgat gaccgacttc gtgccgcctg cctacccgca
     1081 cgagatcgag ctccagaacc tcgtcgcggt cgtcgagtgc acgagcaagg agatggtacc
     1141 caggcgcttc caaaacctcg accgctcgcg cctggtcaag gacatccagg agctcaagag
     1201 cctgctcggg gagcgctagg ccgtgaggcg gccgcgctac ttcaggcgca tcttcgcgct
     1261 gttcctcgca gctgccatgg cgccggccgc cggcgtgtcg gtcgtctata cggcgctcgc
     1321 cgggtcggcc ctcaggcgag aggcctccgg tcggctcgac tcccaggcgg ccgccttcgc
     1381 gagcgctgtg gccgcgatag ccgacggctg cgcctcgggg ctgctctccc tgtcgcagga
     1441 cgccgccgtc ctggccgcgc tctgcggcgg agagccggaa ggggccgccg cggtccgcgc
     1501 cttccgcagc ctgctccgcc tcccggtacc agtcctggcg gcggcgcagc atggcgaaat
     1561 ccaccgcatg gccatcgaaa tcggggccat cgaaacaggc gaacttcacc tcgccgccca
     1621 cggccacccg gcagccgcgg cacatgcc
//
LOCUS       DAOOLN010000462         1642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_96279_length_1642_cov_2.705734, whole genome shotgun sequence.
ACCESSION   DAOOLN010000462 DAOOLN010000000
VERSION     DAOOLN010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96279_length_1642_cov_2.705734"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PLW80_06510"
     CDS             complement(<1..515)
                     /locus_tag="PLW80_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB66191.1"
                     /translation="MKKGKPSGSGAIAKAGNEYRSGDYDAAVLTLGQATVDEDEYVDL
                     AYLLGLSYARLGRFDEALLYLEQVVTQSVPDRRVDQCRMTLAYVYAVTGRAKLAEYEL
                     KKLLESDKRSPQVFSALGHASWKQGKLDDGIAWYAKALELEPENPTALNGYGYLLACA
                     DRDLQKALTCCR"
     gene            complement(512..1234)
                     /gene="surE"
                     /locus_tag="PLW80_06515"
     CDS             complement(512..1234)
                     /gene="surE"
                     /locus_tag="PLW80_06515"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPB66192.1"
                     /translation="MRILLTNDDGYASDGIKALAKALEGEHEVWTLAPDSERSGMSHS
                     MSLRQPLKIRKLAEREYCCSGTPADCVILASHGVLPFSPDVVVSGINRGPNLGTDLVY
                     SGTAAAARQAAMNGVPGIAVSLATYTGPFIYDAAAQMIAERLDYFISLWTPDVFVNIN
                     APVAPAGHRYEVIEAFPSRRVYKDTLKFFDGPDGYSYCFFMDGQVDTHQESGSDEDAI
                     GKGLAAVTRVLAYPCAAGGAAS"
     gene            complement(1266..1601)
                     /locus_tag="PLW80_06520"
     CDS             complement(1266..1601)
                     /locus_tag="PLW80_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66193.1"
                     /translation="MDRRHAHALVDFADFGSRGFAPPPEHGSMTPSGAIVSLVLTGGW
                     ETARLFQSWRPSLTMFAETSGKNSMFISAMSDRAMEEYRSRLEEYERIFGFKPHALEI
                     SVGAGMIAV"
BASE COUNT          287 a          522 c          543 g          290 t
ORIGIN      
        1 cggcaacagg tcaaggcttt ctgcagatcg cggtcggcgc aggcgagcag gtagccgtag
       61 ccattgagcg ccgtgggatt ttcaggctcc agctcgaggg ccttggcgta ccacgcgatg
      121 ccgtcgtcga gctttccctg cttccaactg gcgtgtccga gcgccgagaa gacctggggc
      181 gaccgcttgt cggactcgag cagcttcttg agctcgtact cggccagctt ggcgcgaccg
      241 gtgacggcgt acacgtaagc gagcgtcatg cggcattggt cgacccgacg gtccggcacc
      301 gactgcgtga cgacctgctc caggtacagg agcgcctcgt cgaagcgccc gagcctggcg
      361 tacgagaggc caagcaggta ggccaggtcg acgtactcgt cctcgtcgac ggtcgcctgg
      421 ccgagcgtca ggacggcggc atcgtaatcc ccggaccggt attcgttacc ggccttcgcg
      481 atggcgcccg aacccgacgg cttgcccttt ttcacgacgc cgcgccgccc gccgcgcatg
      541 gatacgccag cacccgtgtg accgccgcaa ggcccttgcc gatcgcgtcc tcgtcgctgc
      601 ccgactcctg gtgcgtgtcc acctggccat ccatgaagaa acagtaggag tacccgtcgg
      661 ggccatcgaa gaatttgagc gtatccttgt agacgcggcg gctcgggaag gcctcgatga
      721 cctcgtagcg gtgccccgcc ggggcgaccg gcgcgttaat gttgacgaaa acgtcgggcg
      781 tccatagcga tatgaagtaa tcgagccgct cggctatcat ctgggcggcc gcatcgtaga
      841 tgaacggacc ggtataggtt gccagcgaaa cggcgatgcc cggtacgccg ttcatggcag
      901 cctgccgagc ggccgcggcg gtacccgagt acacgaggtc ggtgccgagg ttcgggcccc
      961 ggttgatacc ggatacgacg acgtccgggg agaacggaag cacgccgtgc gacgcgagta
     1021 tgacgcagtc ggccggcgtc ccggaacagc agtactcgcg ctcggctagc ttgcgtatct
     1081 tgagcggctg cctgagggac atcgaatggc tcattccgga gcgttccgag tcgggcgcca
     1141 gcgtccagac ctcgtgctcg ccttcgagcg ccttggcgag ggccttgatg ccgtcgctcg
     1201 cgtaaccgtc gtcgttcgtc agtagaattc tcatcgaagc tcctgggccc ttgctcgggc
     1261 gatcgctata cggcgatcat accagccccg accgatattt ccaaggcgtg cggcttaaag
     1321 ccgaagatac gctcgtattc ttcgaggcgc gaccggtact cctccatggc ccggtcggac
     1381 atggcgctga taaacatgga attcttgccg ctggtttcgg caaacattgt cagcgaaggc
     1441 cgccacgact gaaaaagtct ggcggtttcc cagccgccgg tcaggaccag cgagacgatc
     1501 gcgccggaag gggtcatgga gccgtgctcg ggcggcggcg cgaagccgcg gctgccgaag
     1561 tccgcgaagt cgacgagcgc gtgcgcgtga cggcggtcca cggcgacgac tccaggaacg
     1621 gtcgttcact accgtgttac ac
//
LOCUS       DAOOLN010000463         1641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_96335_length_1641_cov_3.791299, whole genome shotgun sequence.
ACCESSION   DAOOLN010000463 DAOOLN010000000
VERSION     DAOOLN010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96335_length_1641_cov_3.791299"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PLW80_06525"
     CDS             complement(<1..285)
                     /locus_tag="PLW80_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66194.1"
                     /translation="MPEVVFSYSRADALADGILVDVSELAREAGFKLPVAVSDTLYHG
                     YLTPPLELAKEGQSLDGRLWDTLSVLRYAIKAASSTDRISFSVLFQMASVA"
     gene            333..605
                     /locus_tag="PLW80_06530"
     CDS             333..605
                     /locus_tag="PLW80_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66195.1"
                     /translation="MFYLNMLDYDDDRGHGIERRVDAKATDEADGGADVERVANDGGE
                     ATVGDGRHGWNVGARVEQEAGGGRAVVGGLREERRRSDRALKEQAV"
     gene            complement(626..1231)
                     /locus_tag="PLW80_06535"
     CDS             complement(626..1231)
                     /locus_tag="PLW80_06535"
                     /inference="COORDINATES: protein motif:HMM:NF019000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HPB66196.1"
                     /translation="MLATLLDAPPTAPLALVDSAEPGPLPSLSNVLFDFTIPSAEAPA
                     AEPFTVDSLSKASWCASRILEAQARTAQRSDLARSYKSRIDTWLSDANAADIDSIEYL
                     SSLLKPWVEAEVAKQRRSRSVLLPTATAQLRKLPDRVDILDAPTALGYLKANHPEAVI
                     VREDISKTAVRSLIFTEGEAIPGVEAQLGHDELYIRPAAQA"
BASE COUNT          340 a          394 c          646 g          261 t
ORIGIN      
        1 agcgacggag gccatctgga agaggactga gaaagaaatg cgatccgttg aggaggcggc
       61 cttgatggcg tagcgaagga cggacagcgt gtcccaaagg cgtccatcca gactctggcc
      121 ttccttggcg agctcgagcg gtggcgttag gtagccgtgg tagagggtgt cggagacggc
      181 cactgggagc ttgaagccag cctcgcgggc gagctcggag acatcgacga ggatgccgtc
      241 ggcgagcgca tcggcccggc tatatgagaa aacaacctca ggcatcacga ggcggcgtcc
      301 tcggcgtagg cgcgtgcttc cggggaatag gaatgtttta cctcaacatg ctcgactacg
      361 atgacgacag gggtcatggc atcgagcgaa gagtcgatgc caaggcgacc gacgaggcgg
      421 acggaggtgc cgacgtggag cgtgtcgcga acgatggcgg cgaggcgacc gtaggcgatg
      481 gccggcatgg ctggaacgtc ggggcccgcg tcgagcagga agcgggcggc ggccgggcgg
      541 tcgttggcgg gctccgagaa gagcggagaa gaagtgacag agccctcaag gagcaggctg
      601 tttaagagac gcatggggtg tcctttcagg cctgggcggc cgggcggata tagagttcat
      661 cgtggcccaa ctgggcttcg acgccgggga tggcttcgcc ttcggtgaag atcaaggagc
      721 ggacggcggt cttcgagatg tcctcgcgga cgatgacagc ttctgggtga ttggccttga
      781 ggtagccgag ggctgtagga gcatcgagaa tgtcgacccg gtcggggagc ttgcgtagct
      841 gggcggtggc cgtcggtagg aggacggagc gggagcggcg ctgcttggcg acttcggctt
      901 cgacccaggg cttaaggagc gaggagaggt actcgatgga gtcgatgtcg gcggcgttgg
      961 cgtcggagag ccaggtatcg atacgcgact tataggagcg ggccaagtcg gagcgctggg
     1021 ccgtgcgggc ctgggcctcg aggatgcgcg aggcgcacca tgaggctttg gagagagagt
     1081 cgaccgtgaa gggctcggcg gccggggcct ccgcgctggg gatggtgaag tcgaagagga
     1141 cgttggagag cgatggaaga gggccgggct ctgcgctgtc gacgagcgcg agcggggcgg
     1201 tagggggagc gtcgagaaga gtggcgagca tgtgagcctc cttgggaaga tgggaaaatg
     1261 ggaagcggcc gggattgagc ctgcccatga ggtaggcgat ggccttatta tgtaattagc
     1321 agctgacggc cggactgtgt acgcgatatg cacggcggcc ggggccggga ccggggctac
     1381 cgagaacgat gaaaacgttt gtaggaatgc tggcgggctt aatcggggcc ataggcgcca
     1441 agcacaatgt gctaaggggc ttagacggat agatgtatta gcaaagctac gacccggagg
     1501 aaggaccgca aggcccccgc gcggggctct tctccgtgcg ctgggaaaac gggcaaaaaa
     1561 gcatacggga aaacggcaag cccataagcg accgcaagcg gctcgtggaa aacaattcct
     1621 cggtgatttt ctgccatgac g
//
LOCUS       DAOOLN010000464         1641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_96357_length_1641_cov_2.990542, whole genome shotgun sequence.
ACCESSION   DAOOLN010000464 DAOOLN010000000
VERSION     DAOOLN010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96357_length_1641_cov_2.990542"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1466
                     /locus_tag="PLW80_06540"
     CDS             <1..1466
                     /locus_tag="PLW80_06540"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPB66197.1"
                     /translation="RYRRDDTHPGMARRGGLIIMDEKRGSVIVVDDDVGVLRRLDRLL
                     RFEGYGEVIMCERADDAWPALRASRPIAMILDLMMPGVDGYATLERMREERPDVPVIV
                     ATAIDDLDTVVRCMKAGAFDYVPKTAETSRLLASLEHAAMIGRLLEDNKALRDSLLHD
                     DKSSIGFFEGIVTRDKKMSDIFRYLAAIAKSDKPILICGESGTGKELFASAAHAASGR
                     SGELVTVNIAGLDDAMFSDTLFGHKRGAFTGADTDRLGLIERAKGGTLFLDEIGDLPQ
                     QSQVKLLRLIEQRRYYPLGADMTRSSDALIVAATNRDLERACAAGEFRPDLYFRLETH
                     LIRLPPLRERRDDLPLLVEVFVARAAEKFGKPAPGVPIELYDLLGAYDFPGNVRELES
                     MVHDAVGRADGRMLSLDSFRRRVFKPGARPVAASPVAKNVFSTLATLPTLREASDALI
                     AEALARSGGNQGVAAGLLGLSRTALNRRIKLRIEEDA"
BASE COUNT          305 a          506 c          541 g          289 t
ORIGIN      
        1 gccggtaccg ccgtgacgat acgcatcccg gtatggctcg gcgcggaggg ctgatcatca
       61 tggacgagaa acgcggttcg gtcatcgtcg tcgacgacga cgtcggagtg ctgaggcggc
      121 tggaccgcct gctgcgcttc gaaggctacg gagaggtgat catgtgcgag cgggccgacg
      181 acgcgtggcc ggcgctgcgc gcgtcccggc cgatagccat gatcctcgac ctgatgatgc
      241 ccggagtcga cggctacgcg acgctcgagc ggatgaggga ggagcggccc gacgtaccgg
      301 tcatcgtcgc cacggcgatc gacgacctgg ataccgtcgt ccgatgcatg aaggcgggcg
      361 ccttcgacta cgtaccgaag acggccgaga cctcgaggct cctggcgagc ctcgagcatg
      421 ccgcgatgat aggcaggctg ctcgaggaca ataaagccct gcgcgacagc ctgctccacg
      481 acgacaagag ctccatcggc ttcttcgagg gcatcgtcac gcgcgacaag aagatgagcg
      541 acatcttcag gtacctggcg gctatcgcca agagcgacaa gcccatcctg atctgtggag
      601 agtcgggcac cggcaaggag ttgttcgcct cggcggccca cgccgcctcg ggacgatcgg
      661 gagagctcgt aacggtcaat atcgccggcc tcgacgacgc gatgttcagc gatacgctgt
      721 ttgggcataa gcggggggcc tttaccggag ccgataccga ccggctcggc ctgatcgagc
      781 gggccaaggg cggcacgctg ttcctcgacg agataggcga ccttccacag cagagccagg
      841 tcaagctact caggttgatc gagcagcgcc gatactaccc tctcggcgct gacatgacac
      901 gctcgtccga tgctctgatc gtcgcggcga cgaaccgcga tctcgagcgg gcctgcgccg
      961 cgggcgagtt caggccggat ctatacttca ggctggagac gcacctgata cggttgccgc
     1021 cgctccgcga gcgccgcgac gacctgccgc tactggtcga agtcttcgtc gccagggccg
     1081 ccgagaaatt cgggaagcct gcgccgggcg tgccgataga actctacgac ttgctcggcg
     1141 cctacgattt ccccggtaac gtgcgcgagc tcgagtcgat ggtgcacgac gctgtaggac
     1201 gcgccgacgg caggatgctg tcgctcgatt cgttccgtcg ccgggtgttc aagccaggcg
     1261 cgcgcccggt cgcggcgtct cccgtggcca agaacgtatt ctctacgctg gctaccctcc
     1321 cgaccctgcg agaggcgtcc gacgcgctga tagcggaagc cctggcgcgc tcgggcggca
     1381 atcagggcgt agccgccggt ctcttgggcc tgtccaggac ggccttgaac cggcgcatca
     1441 agcttcgtat cgaggaagac gcttaaggcg gattccgggt tgcccgctac gtctcagcca
     1501 gtacctatcg ccgccgatga tggggatggc catgcaagaa gggtgtgcaa aattcgacac
     1561 gaggcgcttt tggtacatac tgattatatc attttctgga aagaagatag ctattgatat
     1621 agcttcttgg cagacttgta g
//
LOCUS       DAOOLN010000465         1636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_96778_length_1636_cov_2.616066, whole genome shotgun sequence.
ACCESSION   DAOOLN010000465 DAOOLN010000000
VERSION     DAOOLN010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96778_length_1636_cov_2.616066"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..1188
                     /locus_tag="PLW80_06545"
     CDS             67..1188
                     /locus_tag="PLW80_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HPB66198.1"
                     /translation="MNQIILGPLVVAGAAAALALVIALVDRVVNDYGEVTIDVNHGKR
                     LYKVRGGSPLLGTLASQGVFVPSACGGRGSCGACKVRVLSDVGPVLPTETPYLSPQEL
                     ANGVRLSCQVKLKKDIAVELPDELFSVKKFKATVERLDDVTYDIKDAVFRLDEPREIS
                     FTSGQYVQLVVPPYGTIKESVQRAYSMASRPGDSGRVELLIRLVPGGIATTYVHTALK
                     VGDRVELVGPFGDFRVRDTSAAMVCVAGGSGMAPFKSMFYDMRERDAFPDKDIWYFFG
                     ARSSRDMFYLDELRRLEAEWPRFHFVPALSEPSPEEGWNGETGLITDVLERYVKDRIP
                     RDKGLEGYLCGSPGMINACIAVMTRNGIAEGDIFYDKFA"
     gene            1210..>1636
                     /locus_tag="PLW80_06550"
     CDS             1210..>1636
                     /locus_tag="PLW80_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66199.1"
                     /translation="MKNTTAEIAAYERMKPGAITAQGFLGDDERALADIIEADLEAWR
                     RAGVDPDDAATFLERLESLGQKGLGEPTVVDGRWTVTVGDARGRLPCPFHDGAFHKNS
                     IEAVDGTNGARVVYSDLSIHLLRRHRFCQGLGSPFRLDPE"
BASE COUNT          289 a          533 c          550 g          264 t
ORIGIN      
        1 aggcttcatg gcgatggcct tcatcggctt ctccggcatg atagcggtgc agtaagggag
       61 gcggacgtga atcagatcat actggggccg ctcgtcgtcg ccggcgccgc cgccgccctg
      121 gccctcgtca tagcgttggt ggatcgcgtc gtcaacgact acggcgaggt gaccatagac
      181 gtcaaccacg gcaagcgcct ctacaaggtc aggggcggct cgccgctcct cggcacgctc
      241 gcgtcgcagg gagtcttcgt accgtcggcc tgcggcggcc gcggctcctg tggcgcgtgc
      301 aaggtccgcg tgctgtcgga tgtcgggccg gtactgccta ccgagacgcc ctacctgtcg
      361 ccgcaggagc tggcgaacgg cgttcgccta tcctgtcagg tcaagctcaa gaaggatatc
      421 gccgtcgagc tgcccgacga gctgttcagc gtcaagaagt tcaaggcgac ggtggagcgg
      481 ctcgacgacg tcacctacga catcaaggac gccgtcttca ggctcgacga gcccagggag
      541 atatcgttca cgagcggcca gtacgtccag ctcgtcgtcc cgccctacgg taccatcaag
      601 gagagcgtcc agcgcgccta ctcgatggcg tcgcggccgg gagactccgg gcgcgtcgag
      661 ctactgatac gcctggtacc cggcggcatc gccacgacct acgtacatac cgcgctcaag
      721 gtaggcgacc gggtcgagct ggtaggccca ttcggcgact tcagggtccg cgacacgtcc
      781 gccgcgatgg tctgcgtcgc cggaggctcg ggcatggccc cgttcaagag tatgttctac
      841 gacatgcgcg agcgcgacgc cttcccggat aaggatatat ggtacttctt cggcgcgcgc
      901 tcgagccgcg acatgttcta cctcgacgag ctgcggcggc tcgaggccga gtggccgcgc
      961 ttccacttcg ttccggcgct gtccgagccg agcccggaag aaggctggaa cggcgagacg
     1021 ggcctgatca ccgacgtact cgagcgctac gtgaaagaca ggatcccgcg ggacaagggc
     1081 ctcgagggct acctctgcgg cagccccggc atgatcaacg cctgcatcgc ggtgatgacc
     1141 aggaacggta tcgccgaggg cgacatcttc tacgataaat tcgcctagac tagccgcact
     1201 ggagggcata tgaagaacac gacggccgag atagcagcgt acgagcgcat gaagcctggc
     1261 gccatcacgg cccagggttt cctgggcgac gacgagcgcg cgctcgcgga tataatcgag
     1321 gcggacctgg aggcgtggcg ccgcgccggc gtggacccgg acgacgccgc cacgttcctg
     1381 gagcggctcg agtcgctcgg ccagaaggga ctcggcgaac cgaccgtcgt cgacggccgc
     1441 tggaccgtaa ctgtggggga cgcgcgcggt cggctaccct gccccttcca cgacggcgcc
     1501 ttccacaaga actccataga ggcggtcgac gggacgaacg gcgcgcgcgt cgtatactcg
     1561 gacctgtcga tccacctcct gaggcggcac aggttctgcc agggcctggg tagtccgttc
     1621 aggctggacc ccgagg
//
LOCUS       DAOOLN010000466         1632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_97159_length_1632_cov_2.573874, whole genome shotgun sequence.
ACCESSION   DAOOLN010000466 DAOOLN010000000
VERSION     DAOOLN010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97159_length_1632_cov_2.573874"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PLW80_06555"
     CDS             <1..280
                     /locus_tag="PLW80_06555"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPB66200.1"
                     /translation="AAVTIAYEPVWAIGTGKTATPDDADAVHAYIRGVIAGLYGEAAA
                     KAMCIQYGGSVKPENAAELMAKPNIDGALVGGASLKAESFGPIANFRS"
     gene            464..802
                     /gene="secG"
                     /locus_tag="PLW80_06560"
     CDS             464..802
                     /gene="secG"
                     /locus_tag="PLW80_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPB66201.1"
                     /translation="MGVFGIVLLVLFAIVCLLLIFMVVIQDQEGEGLGGLFSGAGNAA
                     FGSRSSSVVVRFTYILGGLFFVIAFGLAVLNKGSVGNVEDAVRAQGGQATEWWSQESA
                     PATETAPAAE"
     gene            810..1298
                     /locus_tag="PLW80_06565"
     CDS             810..1298
                     /locus_tag="PLW80_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPB66202.1"
                     /translation="MPSRPLSLSDLFPRDRICVDLASADKEALFVELVDLIGSTGSTH
                     FDREDALACVLERESRMSTGIRPGVALPHGKTDGVDRFFGAIGVSRLGIDYDAPGGEP
                     VRIAFMLISPRSMASDHLAILKQLAGLLDAPGFVSGMIAASDPSEAHRIIERYEAQSI
                     KV"
     gene            1309..>1632
                     /locus_tag="PLW80_06570"
     CDS             1309..>1632
                     /locus_tag="PLW80_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPB66203.1"
                     /translation="MKDYYAILGIHQEASLKEIKSAFRKRAKVRHPDIVGDDDEAMRL
                     SIEAYKVLSDPASRREYDRGRLRIVKGEAEGSFDYRRWLMERLDQNEYVAKLVFYDLL
                     HDREDE"
BASE COUNT          309 a          500 c          520 g          303 t
ORIGIN      
        1 ggccgcggtc accatcgcct acgagccggt ctgggccatc ggcaccggca agacggccac
       61 gcccgacgat gccgacgcgg ttcacgcgta tattcgcggc gtgatagccg gtttgtacgg
      121 cgaggccgca gccaaggcca tgtgcatcca gtacggcggc tcggtcaagc cggagaacgc
      181 cgccgagctg atggccaagc cgaacatcga tggcgcgctc gtcgggggcg cctcgctcaa
      241 ggccgagagc ttcggcccga tagcaaattt caggtcgtga gcgtgcgaag ccgcgggcct
      301 ggcgcccgcg gcttttcgtc atgacgcccc ttgcgcggcc gcttcgatct ggtatagtat
      361 cgcgcgaggc ggcgtctacc cggcgcatcg cgccgaatta atccggcgcg atgcgccggg
      421 taggtcgcgt aagccagacc caatacaaat cggagtcaat cagatgggtg ttttcggcat
      481 agtgcttctc gttctcttcg cgatcgtctg cctgttgctc atatttatgg tcgtcatcca
      541 ggatcaggaa ggcgagggcc tcggcgggct cttttccgga gccggcaacg cggcgttcgg
      601 ttcgcgatcg tcgagcgtgg tcgtccgctt cacctacata cttggcggac tattcttcgt
      661 tatagcgttc ggcctcgccg tcctgaacaa gggctccgtc ggcaacgtcg aggacgccgt
      721 gcgcgcccag ggcggccagg caacggaatg gtggagccaa gaatccgccc ccgctaccga
      781 gaccgctccc gccgccgaat aggacgcacg tgccttcgag gccgctctca ctcagcgacc
      841 tgttcccccg ggacaggata tgcgtcgacc tcgcgagcgc cgacaaggaa gccttgttcg
      901 tcgagctcgt cgatctgata ggatcgacgg gctcgacgca tttcgaccga gaggacgcgc
      961 tcgcctgcgt actggagcgg gagagccgca tgtccaccgg tatacgcccc ggcgtcgcgc
     1021 taccccatgg caagaccgat ggcgtcgacc gtttcttcgg ggcgataggc gtctcaaggc
     1081 tcgggatcga ttatgacgct ccgggcggcg agcccgtacg gattgccttc atgctcatat
     1141 cgccgcggtc gatggcgagc gatcatttgg ccatcctgaa acagctcgcg gggctgctcg
     1201 acgccccggg cttcgtctcc gggatgatcg ccgcctcgga tccatcggaa gcccatagga
     1261 tcattgagcg ctatgaagcg cagagcataa aggtttaaag aaacgaacgt gaaagattac
     1321 tacgccatcc tcgggatcca tcaagaagcc tccctgaaag agatcaagag cgcgttcagg
     1381 aaacgggcca aggttcgcca tccagacatc gtcggggacg acgacgaagc gatgcgccta
     1441 tcgatcgagg cgtacaaagt cctatccgat cctgcttcga ggcgggagta cgataggggg
     1501 cgactgcgta tagtcaaggg cgaggccgag ggctccttcg attaccggcg ctggctgatg
     1561 gaacgcctcg accagaacga gtatgtggcc aagctcgtct tctatgattt gctccatgac
     1621 agggaggatg ag
//
LOCUS       DAOOLN010000467         1629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_97419_length_1629_cov_2.812579, whole genome shotgun sequence.
ACCESSION   DAOOLN010000467 DAOOLN010000000
VERSION     DAOOLN010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97419_length_1629_cov_2.812579"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLW80_06575"
     CDS             complement(<1..223)
                     /locus_tag="PLW80_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB66204.1"
                     /translation="MSGLEDRIARIIICEDERIVALDLRAFLQRNGYEVPSLFASAED
                     LLEAVEALEPDLVLMDIHLQGAMDGLEAAA"
     gene            complement(278..646)
                     /locus_tag="PLW80_06580"
     CDS             complement(278..646)
                     /locus_tag="PLW80_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB66205.1"
                     /translation="MAKSILIIDDSAAIRQSISYILQQEGYATVEAGDGVEGLQRLDE
                     GTRFDCIITDVNMPNMDGITFIRKARENPKFKFVPILVLTTESQGGKMNEGKEAGATG
                     WIVKPFSADKLLAVVKKVAE"
     gene            complement(718..>1629)
                     /locus_tag="PLW80_06585"
     CDS             complement(718..>1629)
                     /locus_tag="PLW80_06585"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HPB66206.1"
                     /translation="VSDVEMPRMDGLSFLRKIMETSQIPVVICSSKTEAGSDNAIKAM
                     EYGAVEIIQKPKLGTKQFLEESKVEICDVVKAAYAARHRRRAPAAPIRDVAPKLTADV
                     MLPAPGSGHIIETTEKVVVVGASTGGTEALREFLEVLPEDTPGIVIVQHMPEHFTAAF
                     ARRLDGLCRVSVKEAADNDTVIRGRVLIAPGNQHTLLKRSGARYYVEVKEGPLVCRHR
                     PSVDVLFRSAARYAGNNAVGVIMTGMGDDGSRGMKEMHDAKAWTIAQDEQSCVVFGMP
                     NEAIKLGGVDVVLPLGKIASEVLRAAK"
BASE COUNT          276 a          539 c          480 g          334 t
ORIGIN      
        1 tggcggcggc ctcgaggccg tccatagcgc cctgcaggtg gatatccatc agcacgaggt
       61 ccggttccaa ggcctcgacg gcctcaagca gatcctcggc cgaggcgaag agggacggca
      121 cctcgtagcc attacgctgg aggaaggcgc gaaggtcgag ggcgacgatg cgctcgtcct
      181 cgcagatgat tatacgcgca atgcgatcct cgaggccgct catccgcgcc tcgaggcgtt
      241 ccccgcgctc ggcggacgcg gagtcggcgt acgcatgcta ctcggcgacc ttcttgacga
      301 cggccagtag cttatcggcg ctgaacggct tgactatcca accggtagca ccggcttcct
      361 tgccctcgtt catcttgccg ccctgcgact cggtggtcag cacgaggatc ggcacgaact
      421 taaatttcgg gttctccctg gccttgcgga tgaaggtgat gccgtccatg ttcggcatgt
      481 tgacgtcggt gatgatgcaa tcgaagcggg tgccctcgtc gagccgctgg agcccttcga
      541 cgccgtctcc cgcctctacc gtagcgtagc cttcctgctg caggatatag gaaatgctct
      601 gccgtatggc ggccgaatcg tcgataatca agatactctt cgccatgatc tctcctcggg
      661 ccacgcggct cgacgccgga cccttatgta ttgctagcgc cgttcaaagc gaacgcgtta
      721 cttggccgcc ctcaagacct cggacgctat cttgccgagg ggcaacacca catccacccc
      781 cccgagcttg atcgcctcgt tcggcatgcc gaagaccacg catgactgct cgtcctgggc
      841 tatagtccag gccttcgcgt cgtgcatttc cttcatgcct cgcgagccgt cgtcccccat
      901 gccggtcatg atcacgccga cggcgttatt gccggcgtac ctggccgccg acctgaacag
      961 cacgtcgacg ctcggcctgt gcctgcatac cagcggacct tccttgacct cgacatagta
     1021 gcgggcgccg gatcgcttga gtagtgtatg ctggtttccg ggagcgatca acacccttcc
     1081 gcgaatgacc gtgtcgttat cggcggcttc cttgacgctc acgcggcaga gcccgtccag
     1141 gcgccgcgcg aaagccgcgg tgaagtgctc cggcatgtgc tggacgatga cgatgcccgg
     1201 cgtgtcctcc ggtagcacct cgaggaattc ccgaagcgcc tcggtacccc cggtagacgc
     1261 gcctaccacc acgaccttct cggtcgtctc gatgatatgc cccgagccgg gggcaggcag
     1321 cattacgtcg gcggtgagtt tcggagccac gtcgcgtata ggagcggctg gcgcgcgtct
     1381 acgatgccgc gccgcatagg cggccttgac gacgtcgcag atctcgacct tcgattcctc
     1441 caggaactgc ttcgtgccga gcttcggctt ctggatgatt tcaacagcgc cgtattccat
     1501 cgccttgatg gcgttatccg aaccggcctc ggtcttgctc gagcagatga ccaccggtat
     1561 ttggctggtc tccataatct tccgcaggaa cgaaaggccg tccatcctcg gcatctcgac
     1621 gtcggatac
//
LOCUS       DAOOLN010000468         1628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_97479_length_1628_cov_3.483153, whole genome shotgun sequence.
ACCESSION   DAOOLN010000468 DAOOLN010000000
VERSION     DAOOLN010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97479_length_1628_cov_3.483153"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PLW80_06590"
     CDS             complement(<1..237)
                     /locus_tag="PLW80_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPB66207.1"
                     /translation="MDSKLKDLSTRRERVTAGGGPKRVAAQHDKGKMTARERIEAFLD
                     PGTFTEIDAFVEHRATDLGMAEVEAPGEGVVIGYG"
     gene            complement(279..680)
                     /gene="mce"
                     /locus_tag="PLW80_06595"
     CDS             complement(279..680)
                     /gene="mce"
                     /locus_tag="PLW80_06595"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HPB66208.1"
                     /translation="MVVKVDHIGIAVSNLEETLKVYTDLLGMQLHGTETVEEQKVKTA
                     FLPVGDSEIELLESTAQDGPIAKFIETRGQGIQHIAFKVDDIEAALAELKAKGVRLID
                     EKPRYGAGGAKIAFLHPKATNGVLIELCQKG"
     gene            complement(689..>1628)
                     /gene="meaB"
                     /locus_tag="PLW80_06600"
     CDS             complement(689..>1628)
                     /gene="meaB"
                     /locus_tag="PLW80_06600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPB66209.1"
                     /translation="TDKLVGDLRGRGRKVAVIAVDPSSPFTGGAILGDRIRMQRHATD
                     DGVYIRSLATRGHLGGLSRAASEAVRVLDAAGYDDVIIETVGVGQSEVDIVRIADTVV
                     LVSVPGLGDDIQAIKAGVMEIGDVFCVNKADKDGAERVVREIRAMLETAVLNGAATRF
                     TPLFEARSALRGDAGNAGGATEAVASRGPAEQGAFHHITAQATGHPNLEIPPVVKTVA
                     ETGEGIGPLVDEIKAHEAALAATGFLRSRREANLAWELGRGAAQRVLSELERDESRET
                     LAELARSVLDRGIDYYDALDALEDAMKRTRRAATTA"
BASE COUNT          227 a          560 c          531 g          310 t
ORIGIN      
        1 gccgtagccg atgacgacgc cctcgccggg cgcctcgacc tcggccatgc ccaggtcggt
       61 ggcgcgatgc tctacgaacg cgtcgatctc ggtgaaggta cctggatcga gaaaggcctc
      121 gatccgctcc cgggcggtca tcttgccctt gtcgtgctgt gccgcgacgc gcttggggcc
      181 gccaccggcg gtcacgcgct cgcgtcgcgt agacaggtct ttcagcttgg aatccataaa
      241 attcgctcca tccttccgga aattgcttgt tacagagtct agcctttttg gcaaagctcg
      301 ataagtaccc cattcgtcgc cttggggtgc aagaaggcga tcttggcccc gccggcgccg
      361 taccgcggct tctcgtctat cagcctgacg cccttggcct tgagctcggc gagcgcggcc
      421 tcgatgtcgt cgaccttgaa ggcgatgtgc tggatgccct gtccccgggt ctcgatgaac
      481 ttggctatcg ggccgtcctg ggcggtcgac tcgagcagct ctatctccga gtcgccgacc
      541 ggcaggaagg ccgtcttgac cttctgctcc tcgacggtct cagtgccgtg cagctgcatg
      601 cccaagaggt cggtatacac cttgagcgtc tcttccaggt tcgatacggc aatgccgatg
      661 tggtcaacct tgacgaccat gaatcctcct atgctgtcgt cgccgcgcgc cgggtacgct
      721 tcatcgcgtc ctcgagcgcg tcgagcgcgt cgtagtagtc tatgccccgg tcgaggaccg
      781 accgggccag ctcggccagg gtctcgcggc tctcgtcccg ctcgagttcc gatagcaccc
      841 gctgggcggc tccgcgaccg agttcccaag ccaggttggc ctcgcgcctc gagcgcagga
      901 agcccgtggc ggcgagagcc gcctcgtgcg ccttgatctc gtcgaccagg ggcccgatgc
      961 cctcgcccgt ctccgccacc gtcttgacga ccggcggtat ctcgaggttc gggtggccgg
     1021 tcgcctgcgc ggttatatgg tggaaggcgc cctgctccgc cgggcctctg gacgcgacgg
     1081 cctccgtcgc gccgcccgca ttgccagcgt cgccgcgtag cgccgagcgc gcctcgaata
     1141 gcggcgtgaa gcgggtcgcg gccccgttga gcacggcggt ctcgagcatc gcgcgtatct
     1201 cgcgtaccac gcgctcggcg ccgtccttat cggctttgtt cacgcagaag acgtcgccta
     1261 tctccatgac gccggccttg atcgcctgaa tgtcgtcccc gaggccgggg accgatacga
     1321 gcacgacggt gtcggctatg cgcacgatgt ccacctcgga ctggccgacg ccgacggtct
     1381 cgatgatcac gtcgtcgtag ccggcagcgt cgaggacgcg cacggcctcg ctggcggccc
     1441 tggataggcc gccgaggtgg ccgcgcgtgg ccaggctacg gatatagacg ccgtcgtcgg
     1501 tcgcgtggcg ctgcatacgg atgcggtcgc cgagtatggc gccgccagtg aacgggctgg
     1561 acgggtctac ggcgatgacc gccaccttgc gaccgcggcc gcgcaggtca ccgaccagct
     1621 tgtcggtc
//
LOCUS       DAOOLN010000469         1628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_97485_length_1628_cov_3.130960, whole genome shotgun sequence.
ACCESSION   DAOOLN010000469 DAOOLN010000000
VERSION     DAOOLN010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97485_length_1628_cov_3.130960"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..635)
                     /locus_tag="PLW80_06605"
     CDS             complement(48..635)
                     /locus_tag="PLW80_06605"
                     /inference="COORDINATES: protein motif:HMM:NF014557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HPB66210.1"
                     /translation="MMVIDNGRVSAGPLASLAGLAPLLVFSNSLSQGVALGVAFFLAY
                     SAAGAMALLLPARLGRSLVFMFSILGAAVVSSLTASALRLFDPFLFEAAYDRVFLSVF
                     TLPVLRACVIPATVSDRERAIERLLWGIGYSFLIAVTGAAREFLATGAVSTRFGTERG
                     SLLPIFAQPAGAFMLIALAAAAFTVVIKAVRGSES"
     gene            complement(619..1122)
                     /locus_tag="PLW80_06610"
     CDS             complement(619..1122)
                     /locus_tag="PLW80_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66211.1"
                     /translation="MDVSEKTRDTWILAGAMALIVAASGFASSYASGQAARQLAKMTS
                     EALGPSPRGEATWTTMREGSVLGRVDRRGEPAAYLVVARYADGEFRAAASVDGDGSIL
                     ELKPLGDSGDAYSKRLAVLFSGAVRRGSRADASTLDASLKALVTDTFEALAILERGRT
                     EADDGDR"
     gene            complement(1124..>1628)
                     /locus_tag="PLW80_06615"
     CDS             complement(1124..>1628)
                     /locus_tag="PLW80_06615"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPB66212.1"
                     /translation="FLNDSLFSRLSARLPDGYIDLLIGFKPGALGESALVAVALGSIV
                     LVSLRLIKFEIPLAMLTAFGLLSRIFGTGLPGESFLAGDAMFALSGGSLLLVAFYMAA
                     DPVSSPVGRGVCLLYGASIGALSFIFRRWGAFAEGVAYAVLVMNVTVPFIEKAAASLR
                     LRRGRVA"
BASE COUNT          333 a          537 c          518 g          240 t
ORIGIN      
        1 caggacgtta ccgccgagcg tcaaatagaa cacgaggcca atgacactca tgactccgat
       61 cccctaacgg ccttgataac caccgtgaat gcggccgcgg ccaaggctat gagcatgaac
      121 gccccggcgg gctgtgcgaa gatgggtagc agcgagccgc gttcagtgcc gaaacgcgtc
      181 gagacggctc cggtggctag gaattccctg gcagcgccgg ttacggctat caggaatgaa
      241 tagcctatac cccataagag gcgctctatc gcgcgctccc ggtcagaaac cgtcgctggt
      301 atgacgcagg cgcgcagtac cgggagcgta aaaaccgata ggaaaaccct atcgtaggcg
      361 gcctcgaaca ggaacgggtc gaataggcgc aaagccgagg ccgtcaacga cgagacgacg
      421 gccgcgccta ggatcgagaa catgaacacg agggagcggc cgagccgcgc tggcaggagc
      481 aatgccatgg cgccagcggc gctataggct aggaagaagg cgacgccgag cgcgacgccc
      541 tgcgacaggc tgttcgagaa caccaggagc ggtgctagtc cggcgaggga ggccaagggc
      601 ccggccgata cgcgcccgtt atctatcacc atcatcagcc tccgtcctgc ctcgttccag
      661 tattgccagc gcctcgaacg tatcggtaac gagcgccttg agactcgcgt cgagcgtcga
      721 ggcgtccgcc cgcgaaccgc gcctgactgc gccggagaac agcacggcga gccgcttcga
      781 ataggcgtcg ccggaatccc cgaggggctt cagttcgagt atggatccgt cgccgtcgac
      841 gctggccgcg gccctgaatt caccgtcggc gtaccgagcc accaccaggt aggccgctgg
      901 ctcgccccgc ctgtctaccc tgccgagtac gctcccctcg cgcatagtcg tccaggtggc
      961 ttctccccgg ggcgacgggc cgagcgcctc gctcgtcatc ttggccagct gcctggctgc
     1021 ctgcccggaa gcgtacgagg aggcgaagcc cgaggccgcc acgattagcg ccatggcgcc
     1081 ggccagaatc caggtatccc tcgtcttttc ggatacgtcc atactacgcg accctcccgc
     1141 gcctcagccg caggctcgcg gcggccttct cgatgaacgg caccgtcacg ttcatgacga
     1201 gcacggcgta cgcgacgcct tcggcgaagg cgccccagcg cctgaatatg aagctcagcg
     1261 cgcctatcga ggcgccgtac aggaggcaga cgccccgccc taccggcgag ctgacgggat
     1321 cggccgccat atagaatgcc acgagcagga ggctacctcc cgacagggcg aacatggcgt
     1381 ctcctgccag gaagctctct cctggcaagc cggttccgaa gatcctggat aggagaccga
     1441 aggccgtgag catcgccaga ggtatctcga actttataag cctgaggctg acgaggacga
     1501 tggacccgag ggccacggct accaaggcgc tttcgccgag ggccccgggc ttgaagccga
     1561 tcagcaggtc gatgtagccg tcgggcagcc tcgccgatag cctcgagaac agcgaatcgt
     1621 tcaggaag
//
LOCUS       DAOOLN010000470         1627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_97591_length_1627_cov_2.929389, whole genome shotgun sequence.
ACCESSION   DAOOLN010000470 DAOOLN010000000
VERSION     DAOOLN010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97591_length_1627_cov_2.929389"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1525)
                     /locus_tag="PLW80_06620"
     CDS             complement(128..1525)
                     /locus_tag="PLW80_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPB66213.1"
                     /translation="MDDSMLDRVFYHIYPLGAFGAPRRNDGSSHPEPRLRRLAEWIPA
                     MERIGADALYLGPVFESESHGYDTLDYLMVDRRLGDGGDLAEASRALASRGIGLYLDA
                     VFNHVGRGHPFVRDVVERGHASPYARWIAGYDPSGPGPGGLPFGYDGWKGHYDLVRLD
                     TGLPEVREYLIGVALRWIDEYGVAGLRLDAADCLDRLFMAELGRRCRVARPGFRLVGE
                     VVHGDAYEPILREGGLDAVTDYEAYKGLWSSYNDRNYHEIAWTLDRLFAEPVAGPASP
                     GTWSGAGLCRGRAMYSFADNHDVDRVASLIRDPAGLYPLYALLFSMPGCPSVYYGSEY
                     GVTGRKAATDDGPLRPALDPAALASAAPHPDLAAAVVRFSEARRRSSALRRGSYRRLA
                     VGNLHLAFERRAGDEAVLVAVNASQEPVRLELPAGLPDGPVVDLLDGGYHEESRGGRL
                     ATVVPPHWARWLRKE"
BASE COUNT          257 a          563 c          581 g          226 t
ORIGIN      
        1 ggcggcccga gacgcgatag cccgcgccgc gccgttccca cccggcgacg tcgggccaac
       61 cgagtaccgg gtcaccatcg tctataaact ggagcgctga gcgcgatggg tgttgcgcgg
      121 catagcgcta ctccttcctc agccacctgg cccaatgcgg cggcactacg gtcgcgagcc
      181 tgcctccccg gctctcctcg tggtatccgc cgtcgaggag gtcgacgacg ggcccgtcgg
      241 ggaggccggc tggcaactcc aggcgtacgg gctcctggga tgcgttcacc gcgacgagca
      301 cggcctcgtc gccggcccgc cgctcgaacg cgagatgcag gttacccacg gccaggcggc
      361 ggtaggagcc ccgccggagc gccgacgagc ggcgcctggc ctcggagaat cgcacgacgg
      421 cggccgccag gtccgggtgg ggcgcggccg aggccagggc ggccgggtcg agcgccgggc
      481 gtagcgggcc gtcgtcggtg gcggccttgc gccccgttac gccgtactcg ctgccgtagt
      541 agacggacgg gcagccgggc atggagaaca agagcgcgta cagagggtag agcccagccg
      601 gatcccgaat caggctcgcc acgcggtcga cgtcgtggtt gtcggcgaac gagtacatgg
      661 cccggccgcg gcacaggccc gccccggacc aggtacctgg agaggccggg ccggccacgg
      721 gttcggcgaa caggcggtcg agggtccatg cgatctcgtg gtaattgcgg tcgttatagc
      781 tcgaccacag gcctttgtag gcctcgtagt cggtcaccgc gtcgagcccg ccctcgcgca
      841 ggatcggctc gtaggcgtcg ccgtggacga cctcgccgac gaggcggaaa cccggcctcg
      901 ccaccctgca gcgtctaccg agctctgcca tgaaaaggcg gtccaggcag tcggccgcgt
      961 cgagcctgag cccggcgacg ccgtactcgt cgatccagcg tagcgcgacg ccgatcaggt
     1021 actcgcggac ctccggcagg ccagtgtcca ggcgcaccag gtcgtagtgg cccttccacc
     1081 catcgtagcc gaacggcagg ccgccgggcc cggggccgga cgggtcgtag ccggctatcc
     1141 agcgcgcgta gggcgaggcg tggccccgct cgacgacgtc gcgcacgaag ggatggccgc
     1201 gcccgacgtg gttgaacacc gcgtcgaggt acaggccgat gccgcgcgag gcgagggccc
     1261 gggacgcctc ggccaggtca ccgccgtcgc cgagccgccg gtcgaccatc aggtagtcga
     1321 gcgtgtcgta gccgtggctc tccgactcga agaccggccc caggtacagc gcgtccgcgc
     1381 ctatccgctc catcgcaggg atccattcgg ccaggcgacg tagccgcggc tctgggtgcg
     1441 agctgccgtc gttccgcctc ggagcgccga atgccccgag cggataaata tggtagaaaa
     1501 ctcggtcgag catcgaatcg tccatggtcg ctttcctcta cgagaagatc ccgtgcatat
     1561 agtagtgaat ggcacgccgg aggaaggcct cgtcagggtc caggtctcca gctaggtaca
     1621 ggccgac
//
LOCUS       DAOOLN010000471         1627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_97607_length_1627_cov_2.545165, whole genome shotgun sequence.
ACCESSION   DAOOLN010000471 DAOOLN010000000
VERSION     DAOOLN010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97607_length_1627_cov_2.545165"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="PLW80_06625"
     CDS             complement(<1..700)
                     /locus_tag="PLW80_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPB66214.1"
                     /translation="MIKRTNDSVPTVAPMVPRSSAQMAAPVGLWLAFILFTVAVGTVD
                     REAIGPDGSVVGLAAMNRLIYELLEAQPFWYALTDWLGLVPVAVACGFACLGAWQLVR
                     RRSLRRVDADIMLLGALYLAVVGYYVLFERLVVNYRPILIGGRLEASYPSSHTMVAVG
                     VMATAVLQFRRRTRSHRLLAALELASAAVIAVTALGRLISGAHWLSDIIGGLLLGSAL
                     AASYRAALARRRSVS"
     gene            complement(785..1390)
                     /locus_tag="PLW80_06630"
     CDS             complement(785..1390)
                     /locus_tag="PLW80_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPB66215.1"
                     /translation="MEFNEKLRSLRARAGLTQERLADELCVSRVTVSKWESGRGYPNI
                     ESLRLMAKTLSVSIDDLLSGDELLSVAESQSRESSGGLLAVAFGVLDVMMTLLLALPL
                     FGDEIGGRIASVALPRVTGMPPYIVAAYYVAVVATATFGVLQLALQNLRSPAWRRLSL
                     PASVGLSVLALLFFIMTRQPYVSAFSLCLLLLKGILLIKRR"
     gene            complement(1398..>1627)
                     /locus_tag="PLW80_06635"
     CDS             complement(1398..>1627)
                     /locus_tag="PLW80_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66216.1"
                     /translation="YHPSASIGGWTFGLLPGFVYSFDSRTAGFSASLNCGYKWVIGNG
                     LILGALTGARYVWIDGYIVLPDLAISLGYRL"
BASE COUNT          321 a          543 c          535 g          228 t
ORIGIN      
        1 aggatacgga gcggcgccgc gccagggccg cgcggtacga cgcggccagc gccgaaccca
       61 ggagcaggcc gccgatgata tccgacagcc agtgggcgcc ggagatcagc cgcccgagcg
      121 cggtaacggc gatgacggcg gccgacgcca gttcgagcgc cgcgagcagg cggtggctcc
      181 gggtccgcct gcggaactgc agcacggccg tcgccatgac gcctacggcc accatggtat
      241 ggctcgacgg gtacgacgcc tctagccggc cgccgatcag gataggcctg taattgacga
      301 cgaggcgctc gaacaggacg tagtagccga cgaccgccag gtacaacgcc ccgagcagca
      361 tgatatccgc gtcgacgcgc cgcaggctcc ggcgccggac cagctgccat gccccgaggc
      421 aggcgaagcc gcaggccacg gctaccggca ccaggcccag ccagtcggtc agggcgtacc
      481 agaacggctg cgcttcgagc agctcgtaga ttaatcggtt catggccgcg aggccgacga
      541 ccgacccgtc cggtcctatc gcctcccggt cgaccgtgcc gaccgcgacg gtgaatagta
      601 tgaacgccag ccaaaggccg accggcgcgg ccatctgtgc cgacgaccga ggcaccatcg
      661 gagccacggt cgggaccgag tcattcgttc gcttgatcat gattgatcct ccctccgcgc
      721 attaggccgc ggacgggagg gtatcggcca gaaacgaagc cttacatcgg agatactttt
      781 agtatcagcg gcgcttgatg agcagaatcc ccttgagtag gagcaggcag agcgagaagg
      841 cgctgacgta gggctggcgg gtcatgatga agaacagcag cgccaggacc gacagcccga
      901 ccgaagcggg caggctcagg cggcgccagg ccgggctccg caggttctgc agggcgagtt
      961 gcagcacacc gaaggtcgcg gtggcgacca cggcgacgta gtacgccgcc acgatataag
     1021 gcggcatgcc ggtcacccgg ggcaaggcca cggacgcgat ccgcccgccg atctcgtctc
     1081 cgaatagcgg cagggcgagg agcagcgtca tcatcacgtc cagcacgccg aaggccacgg
     1141 cgaggaggcc gcccgacgac tccctggact ggctctcggc gacggacagg agttcgtcgc
     1201 cggacagcag gtcgtcgatc gacaccgaca gggtcttggc catcagcctg agcgattcga
     1261 tattcggata gccgcgcccc gactcccatt tggagaccgt gaccctcgac acgcacagtt
     1321 cgtcggcgag ccgttcctgg gtcaggccgg cccttgccct caggcttcgc agcttctcgt
     1381 tgaattccat ccgccgttta tagcctatat cccaggctga tcgccaggtc agggaggacg
     1441 atgtacccgt cgatccatac gtaccgggct ccggtcaacg ccccgaggat caggccgttc
     1501 ccgatcaccc acttgtatcc gcagttcagc gaggccgaga atccggccgt cctcgaatcg
     1561 aatgaataga cgaagcccgg tagcaatccg aaggtccacc cgccgatcga cgcggacgga
     1621 tggtacg
//
LOCUS       DAOOLN010000472         1625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_97763_length_1625_cov_3.256688, whole genome shotgun sequence.
ACCESSION   DAOOLN010000472 DAOOLN010000000
VERSION     DAOOLN010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97763_length_1625_cov_3.256688"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..843)
                     /locus_tag="PLW80_06640"
     CDS             complement(175..843)
                     /locus_tag="PLW80_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB66217.1"
                     /translation="MSLGVDATVTIAIVEDQREVREGLTYLLGLDGRVRVIGGFDAAE
                     GLLDWMAGAEKRDEPDIVLMDIHLPGMDGIEATRVLRSARPNMVVLILTVFEEPDTIL
                     AAFRAGARGYLLKNTRPEALLDQVLSALDDGSPISPTVARRLLDELRRDADEGASADY
                     GLTAREREVLRDIVDGLTYKELAEKHHIAGSTAKKHMLHIYQKLNVSSKAEIVRKALS
                     EHLV"
     gene            complement(840..>1625)
                     /locus_tag="PLW80_06645"
     CDS             complement(840..>1625)
                     /locus_tag="PLW80_06645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPB66218.1"
                     /translation="AQVASSDEFGLLATEFNRLLDAVEENSSSLEGKVRLRTAELGRL
                     QRENTRLKVIEERRRIYRDLHDTIGAKLTNIFFCNGVALDLAKDGPERLKAMLEGVES
                     NCLQAVDSLKRVILGMGEDDANAGSFSLFVSAGVRQRLAARGMAFDCVIKDKRAIDAL
                     PAGLRDELERILDELVSNALKHSEAGSVRLRLHLRAGALHLAFSDDGVGFDPRARSSG
                     SGLGNIRYRVEGLGGTLSVDTAEGQGSGYRIVVPVDGLPEAQS"
BASE COUNT          266 a          546 c          507 g          306 t
ORIGIN      
        1 caggagattg gtcttgaagt accaatcgag cgcgcccttc ttcttggcgt tttctgtagc
       61 catggatgcc tccggatacg tttggtacgc ccccggcaag gaggcgtgga tcgatacgcg
      121 aagtgtaagc cctgatttcg gatcgcgata tgcaccaaaa ggagtatttt acgcctagac
      181 caggtgctcg ctcagcgcct tgcgtacgat ctcggccttg gacgatacgt tcagcttctg
      241 atagatatgg agcatgtgct tcttggccgt ggagccggcg atgtggtgct tctcggccag
      301 ctccttgtag gtcaacccgt cgacgatgtc gcggagcacc tcgcgctccc tagcggtcag
      361 gccgtagtcg gccgaagcgc cctcgtcggc gtcgcggcgc aactcgtcga gcagccgcct
      421 ggccacggtg gggctgatcg gcgagccgtc gtcgagggcg ctcaacacct ggtcgagcag
      481 cgcctcgggg cgggtattct tgagaaggta gcccctcgct ccggccctga aggccgccag
      541 tatggtatcg ggttcctcga acacggtcag gatcagcacg accatgttcg gacgggcgga
      601 ccgcaggacg cgggtcgcct cgatgccgtc catgccgggc agatggatat ccatgagtac
      661 gatatccggc tcgtcgcgct tctcggcccc ggccatccaa tcgagcagcc cttcggccgc
      721 gtcgaagccg ccgattaccc gcacccgccc atccagcccg agcaggtagg tcaggccttc
      781 acgcacctcg cgctgatcct cgacgatggc tatggtcacg gtcgcgtcga ctccgaggct
      841 catgattgcg cctccggcag gccgtcgacc ggcacgacga tgcggtagcc ggagccttgg
      901 ccctcggccg tatcgacgct cagcgttccg cccagtcctt ccacccgata ccgaatattc
      961 ccgaggcccg aaccggagga cctggccctc gggtcgaagc cgacgccgtc gtcggagaac
     1021 gccagatgca gggcgccggc tctcaggtgc agacgcagcc tgaccgagcc cgcctcggag
     1081 tgcttgagcg cgttgctgac cagctcgtcc aggatgcgct ccagttcgtc gcgcagcccg
     1141 gcgggcagtg catctatggc gcgcttgtcc ttgatgacgc agtcgaacgc catgccgcga
     1201 gcggccaggc gctgcctgac gcccgccgat acgaataggg agaacgagcc ggcattcgcg
     1261 tcgtcctcgc ccatacccag gatcacgcgc ttgaggctgt ccaccgcctg taggcagttc
     1321 gactcgactc cctccagcat ggccttgagc cgctcgggcc catccttggc caggtcgagg
     1381 gccacgccgt tgcagaagaa tatattcgtc agcttggcgc cgatggtgtc atgaaggtcg
     1441 cggtaaatgc gtcttcgctc ctcgatcacc ttgagcctcg tgttctcccg ctgcagccgc
     1501 ccgagctccg cggtgcgcag ccgcaccttg ccctccaggc tcgacgagtt ctcctcgacc
     1561 gcgtccagga ggcggttgaa ctcggtggcc aaaaggccga actcgtcgga gctcgctacc
     1621 tgagc
//
LOCUS       DAOOLN010000473         1625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_97768_length_1625_cov_3.023567, whole genome shotgun sequence.
ACCESSION   DAOOLN010000473 DAOOLN010000000
VERSION     DAOOLN010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97768_length_1625_cov_3.023567"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PLW80_06650"
     CDS             complement(<1..429)
                     /locus_tag="PLW80_06650"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB66219.1"
                     /translation="MINIREYELLSLETGAYRDIELDILKETLATWKERPGNPFELIE
                     LRDGATLAGFCLYYRAPNTDYTFDVHTFVVGRDYRNKAVGPRLVELLEERLLESRNYA
                     VIRTETSKLKEDSVGEGFFQSNGFQTIGHIPAFYDADNDYY"
     gene            697..>1625
                     /locus_tag="PLW80_06655"
     CDS             697..>1625
                     /locus_tag="PLW80_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB66220.1"
                     /translation="MTEYTLYNADIASGDVVRARGSVSVRGSRLAAGATGPSFDLGGS
                     SFVYPALINVHDHFRGNYLPRVGPKEDKYYINWAPWDADLKSSPVFDERSNISVEQMY
                     FLSAYKNLFSGVATANDHFPHEINGAYLPRLPIRVIEEYTLSHECSSFDLHWGEGIEI
                     EHARAKERGWPHITHLEEGFDPESQDGIPILEKLGCLDDHCVFIHCIGFSDDDIAKTA
                     KAGASVSWCPASNMFMFNATCKIRKMLRAGINVAIGTDSTATGSVNLLAEMKFARETY
                     REMYGEDLPASVLFKMVTANPARRAAGYLSHTS"
BASE COUNT          327 a          487 c          471 g          340 t
ORIGIN      
        1 gtagtagtcg ttgtccgcat cgtagaacgc cggtatatgg ccgatggtct ggaatccgtt
       61 cgactggaag aacccctcgc cgactgaatc ctccttgagc ttgctcgtct cggtgcggat
      121 caccgcgtag ttcctggact cgagcagccg ctcctcgagc agctcgacga ggcgggggcc
      181 gaccgccttg ttcctgtagt cgcgcccgac gacgaaggtg tgcacgtcga aggtatagtc
      241 ggtgttcggc gcgcggtagt acaggcagaa gccggccagc gtcgctccgt cgcgcagctc
      301 tatgagctcg aacgggttgc cgggcctctc tttccaggtg gcaagcgttt ccttgagtat
      361 atcgagctcg atgtcccggt aggccccggt ctcgagcgat agcagttcgt attcacgaat
      421 gtttatcatt cacttcttct ccgagcgatt cgccgatgac ggcgttttta tactgcgaca
      481 cgaaccatac caggcgattt taatctataa agccttgatt gtatacaata tcggcatatt
      541 atacgtatac cattcgtcgc gcgctcgcga tggacattat cggtcaatgt tttagggtat
      601 cgggccgaaa tataataaga gcagaaaacg aagtgtcggt gcccgccgta tccgccgtga
      661 cggatccgta ggcggccgag gcaaggatgg aatgcagtga cggaatatac tttatataac
      721 gccgacatag cgagcggcga cgtcgtccgc gcccgcggct ccgtatcggt acgcggttcc
      781 aggctcgccg caggggcgac cggcccctcc ttcgacctgg gaggctcctc gttcgtctat
      841 cctgccctga tcaatgtcca cgatcacttc aggggaaatt acctgcctcg cgtcggcccg
      901 aaggaagaca agtactacat caactgggcc ccgtgggacg ccgacctcaa gtcatcgccc
      961 gtgttcgacg agcgttcgaa cataagcgtc gagcagatgt acttcctttc ggcgtataag
     1021 aatctctttt ccggcgtggc caccgccaac gaccatttcc cccatgagat caacggcgcg
     1081 taccttccga ggctccccat ccgagtcatc gaggagtata cgctgtcgca tgagtgctcg
     1141 tccttcgacc tccattgggg agagggcatc gagatcgagc acgcgcgcgc caaggagcgg
     1201 ggctggcctc atataacgca cctcgaggag ggcttcgacc cggagtccca agacggcata
     1261 ccgatactcg agaagctcgg ttgcctcgac gaccattgcg tgttcatcca ctgcatcggc
     1321 ttctccgacg acgacatagc caagacggcc aaggccggcg cgtccgtgtc gtggtgcccg
     1381 gcttcgaaca tgttcatgtt caacgcgacc tgcaagatac gcaagatgct gcgcgccggg
     1441 attaacgtgg ccatcggcac ggattcgacc gcgaccggat cggtcaacct gctggccgag
     1501 atgaaattcg cccgcgagac ctaccgggag atgtacggcg aggacctgcc cgcctccgtc
     1561 ctgttcaaga tggtcacggc gaacccggcc aggagggccg cgggatattt gagccacact
     1621 tcgat
//
LOCUS       DAOOLN010000474         1623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_97964_length_1623_cov_2.686862, whole genome shotgun sequence.
ACCESSION   DAOOLN010000474 DAOOLN010000000
VERSION     DAOOLN010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97964_length_1623_cov_2.686862"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1623)
                     /locus_tag="PLW80_06660"
     CDS             complement(<1..>1623)
                     /locus_tag="PLW80_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPB66221.1"
                     /translation="ARNMEEFVEKMRAVPPECLLYHATRNHFSAWLMARGEVRFARII
                     RNYLPEDFAGPTELRDFICRALDDLQRGKARGVIPAIGANFGDRGLVRLGGGSVGGKG
                     RGLAFIKSLIDNLAFPNRGGGVDIRLPYTTFIGIDEFERFMDQHGLWGYSWYSAPEGD
                     VRAAFLAHPLDPELVSRLRTYLASTDKPLAVRSSGLFEDMLMVPFSGVYDTFLIPNAH
                     PDPERRLSQLCDAVRLIYASLYSTTARAYFDASGYKIEEERMAVVIQELVGTRRGRWY
                     YPLISGTAQSYNYYPVSYLKSDDGLCVAALGLGPYVVEGGEAHRFCPRYPKLDVVPPG
                     RGLGGTQRWFYALDMERLDFDLTQGEEATLSRLDVAEAEADPGFAMVASTWSVEDDRL
                     EPGVSAKGPRIVDFAPVLKYDSFPFAEVVEAVLEVGTKSMGIPVEIEYAVSDDGPGGA
                     PVFYMLQIKPLIQRSDRVSVDLGAIVRGQCLVASERCMGNGRVRGVRDIVYVDPTRWN
                     PAESGAVAAEIAGLNERLKAEGRPYALVGPGR"
BASE COUNT          268 a          525 c          579 g          251 t
ORIGIN      
        1 catcgtcctg gaccgacgag ggcgtaaggg cgtccctcgg ccttcagcct ctcgttcagg
       61 ccggctatct cggccgcgac ggccccgctc tccgctgggt tccagcgcgt ggggtcgacg
      121 tacacgatat cgcggacgcc gcggacgcgg ccgttgccca tgcagcgctc gctcgccacg
      181 aggcactgac cccgcacgat ggcgccgagg tcgaccgaga cccggtccga tcgctggatc
      241 agcggcttga tctggagcat gtagaagacc ggcgccccgc ccggcccgtc gtcgctgacg
      301 gcgtattcga tctcaaccgg tatacccatg ctcttggtgc cgacctcgag gacggcctcg
      361 acgacctcgg cgaaggggaa cgagtcgtac ttgagcaccg gggcgaagtc gacgatgcgc
      421 ggccccttgg cggagacgcc cggctccagc ctgtcgtcct cgacgctcca ggtgctggcg
      481 accatcgcga agccgggatc ggcttcggcc tcggcgacgt cgagccgcga tagcgtggct
      541 tcctcgccct gagtcaggtc gaagtcgagc cgctccatgt cgagggcgta gaaccagcgc
      601 tgggtgccgc cgaggccccg gcccggcggt acgacgtcga gcttggggta gcggggacag
      661 aagcgatggg cttcgccgcc ctcgacgacg tacggcccga gtccgagggc ggccacgcag
      721 agaccgtcgt cggacttaag gtacgagacg ggatagtagt tataggactg ggccgtgccg
      781 gatatcagcg ggtagtacca gcgcccgcgc ctggtgccga ccagctcctg gatcacgacc
      841 gccatacgct cctcctcgat cttgtagccg gacgcgtcga agtaggcgcg ggcggtcgtc
      901 gaatagaggc tggcgtagat gagccggacg gcgtcgcaga gctgcgaaag gcgccgctcg
      961 gggtcgggat gggcgttcgg gatcaggaag gtatcgtaga cgccagagaa cggcaccatg
     1021 agcatgtcct cgaacaggcc ggaggaccgt acggcgagcg gcttatcggt cgaggccagg
     1081 taggtccgca acctggagac cagctccggg tcgagcgggt gcgccaggaa ggcggcgcga
     1141 acgtcgccct cgggggcgga gtaccacgaa tagccccaga ggccgtgctg gtccatgaag
     1201 cgctcgaact cgtctatgcc tatgaacgtc gtgtacggca gcctgatgtc gacgccgccg
     1261 ccccggttcg ggaaggccag gttgtcgatc aggctcttga tgaaggccag cccgcggcct
     1321 ttgccgccga ccgagccgcc gccgagcctg accaggccac ggtcgccgaa attggcgccg
     1381 atggccggga tgacgccgcg cgccttgccg cgctgcaggt cgtcgagggc tcggcagatg
     1441 aagtcgcgaa gctcggtcgg cccggcgaaa tcctcgggca ggtagttgcg gatgatgcgg
     1501 gcgaagcgaa cctcgcccct ggccatcagc caggcggaga agtggttgcg ggtcgcgtgg
     1561 tacaggaggc actcgggcgg tacggcccgc atcttctcga cgaattcctc catgttcctg
     1621 gcc
//
LOCUS       DAOOLN010000475         1622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_98040_length_1622_cov_2.865986, whole genome shotgun sequence.
ACCESSION   DAOOLN010000475 DAOOLN010000000
VERSION     DAOOLN010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98040_length_1622_cov_2.865986"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="PLW80_06665"
     CDS             <1..889
                     /locus_tag="PLW80_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPB66222.1"
                     /translation="IVKFRDDNGVIKSRDELKKVSGMGEKTFEQCAGFLKIPESANPL
                     DNSWVHPERYALASEILPLVKAGSDPSREQRSGLKEKYGVGDTTIDDILLELKKPNRD
                     PRDELPKPILSQGVLAFEDLKEGMKVTGKVKNVVDFGAFVDIGIKESALIHVSELSDR
                     FVTDPMEVIKVGDVHEFRIISLDIVRKRIGLSLKSEGGAAAGGARGQAEGSGPNPSRR
                     VVAVRRPDGQGGPANPANPAGQAGHAGRSERRPDMQRGYGGPRPSGDRDNQRSGGKIE
                     KDDDGTTYNPFAELLKGKK"
     gene            896..>1622
                     /locus_tag="PLW80_06670"
     CDS             896..>1622
                     /locus_tag="PLW80_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66223.1"
                     /translation="MSARNLAGLGLSYAGIFLVIALASALLKAGRIRPSASRKIVHIS
                     VAHWWLLMMATMDTLWIALIGPVSFIVLNALSLRLRLFKAMEDPRPSRNYGTVYFPIS
                     LLVLVLACYARFMPLWVGGIGILIMGWGDGLAALLGEHFGRANTRIFGSVKSAVGAAA
                     MFGASFAVTFAMGLAFDPAAGLGAHLMRAAATAVFATLVELLTPFGVDNITVPILSAF
                     FYQYVAAGPGLSPFLIAAGANALF"
BASE COUNT          311 a          508 c          507 g          296 t
ORIGIN      
        1 gatcgtcaag ttcagggacg acaacggcgt cataaaaagc cgcgatgagc ttaagaaagt
       61 atccggcatg ggcgagaaga ccttcgagca gtgcgcgggc ttcttgaaga tacccgagag
      121 cgccaatccc ctcgacaaca gctgggtgca tcccgagcgc tacgcgctgg cttccgagat
      181 actgcccctg gtaaaggccg gtagcgatcc cagccgcgag cagcgctccg gccttaagga
      241 aaaatacggc gttggcgata ccaccataga cgacatactc ctggaactga agaagcccaa
      301 ccgcgatccc cgcgacgaat tgcccaagcc catactgtcc cagggcgtgc tcgccttcga
      361 ggacctgaaa gagggcatga aggtgaccgg caaggtgaag aacgtcgtgg acttcggcgc
      421 cttcgtcgac ataggcataa aggagagcgc gcttatccac gtgtccgagc tctccgaccg
      481 cttcgtcacc gaccccatgg aagtgatcaa ggtgggcgac gtgcatgagt tccgcatcat
      541 tagcctggat atcgttcgta agcgcatagg cttgtcgctc aagagcgaag gcggggcggc
      601 cgccggcggc gcgcggggcc aagcggaagg ctccggccct aatccctcgc gccgtgtcgt
      661 ggccgttcga aggcccgacg ggcagggcgg gccggccaat ccggccaatc cggcgggaca
      721 agccggccat gccggacgct cggagcggcg gccggatatg cagcgcggct acggcggccc
      781 gcggccttcc ggcgatcggg ataaccagcg cagcggcggt aagatcgaga aggacgacga
      841 cggcacgacc tacaacccct tcgcagagct gctcaagggt aagaagtaaa agtatatgag
      901 cgcgaggaac ctggcgggat taggcttaag ctatgcgggc atattcctcg ttatagcgct
      961 ggcctccgcc ctgcttaagg ccggcaggat acggccctcc gcctcgcgca agatcgtgca
     1021 cataagcgtg gcccactggt ggctcctcat gatggccacg atggatacgc tctggatagc
     1081 gctcataggg ccagtgagct tcatagtcct caatgccctg tcgctgcggc tgcgcctctt
     1141 caaagctatg gaagacccgc ggccgagcag gaattacggc acggtgtact ttcctatttc
     1201 gctcctcgtt ttggtgcttg cctgctatgc caggttcatg ccgctctggg tgggcggcat
     1261 aggcatactg atcatgggct ggggcgacgg cctggccgcc ctcttgggcg agcacttcgg
     1321 ccgcgccaat acccgtattt tcggctcggt gaagagcgcc gtgggagccg cggccatgtt
     1381 cggcgcgagc ttcgccgtta cctttgccat gggcttggcc ttcgatcccg cggccggcct
     1441 gggagcccac cttatgcggg cggcggccac cgcagtattc gccaccctag tggaactcct
     1501 tacgcccttc ggcgtcgaca atataaccgt tcccatactg agcgccttct tctaccagta
     1561 cgtggccgcg ggtcccggcc tctcgccctt cctgatagcc gcgggtgcta acgccctctt
     1621 tg
//
LOCUS       DAOOLN010000476         1621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_98119_length_1621_cov_3.425287, whole genome shotgun sequence.
ACCESSION   DAOOLN010000476 DAOOLN010000000
VERSION     DAOOLN010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98119_length_1621_cov_3.425287"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="PLW80_06675"
     CDS             complement(<1..1099)
                     /locus_tag="PLW80_06675"
                     /inference="COORDINATES: protein motif:HMM:NF019585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HPB66224.1"
                     /translation="MATIRRYYERPAIAELDSVVTGVSERADGASVVTLSETIFYPEG
                     GGQPCDLGDLAGLPLEAALEEGDEVAHILRADPVALAAAGVCPGATVRSVVDLGRRID
                     HSEQHTAQHLLSSILLRAMGARTLSFHLGERHSFIDLNIAPFGRDDADAIEDEVTRAA
                     LDDYGVTTHLCPPEDPASFPLRKEPSVEAAVLRVIEIDGIEYSACCGTHVVSTRSLEP
                     FRILRVEKYKGGCRVYFVAGGRARADYRRVAGIAVRAAAAAGCADDELEAAVRSSRER
                     QKELERSLAVALDEVAAARAEALDGSGDRSGYDSPAEPVFAVAASYDAGNRLARALAR
                     RGRVAVVDCGDELKVVACSPTGGPAVDAAYSQ"
     gene            complement(1165..1437)
                     /locus_tag="PLW80_06680"
     CDS             complement(1165..1437)
                     /locus_tag="PLW80_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66225.1"
                     /translation="MLRLARDELDAKRVQSAIEALDRYLSLFPYGGDEAYYLYALACE
                     QDTPFRDIKRSYEYYKRVRDQYPRSPRWKAAAERVAYLERHYFGLR"
BASE COUNT          273 a          561 c          526 g          261 t
ORIGIN      
        1 cctggctata agccgcatcg acggccggac cgccggtcgg cgagcaggcc acgaccttga
       61 gctcgtcgcc gcagtcgacc acggctaccc ggccgcgcct ggcgagcgct ctggccagcc
      121 ggttgcccgc gtcatagctg gccgccaccg cgaagaccgg ctcggccggc gaatcgtagc
      181 ccgacctgtc gcccgacccg tcgagcgcct cggctctggc cgccgcgacc tcgtcgagcg
      241 ccaccgccag ggaacgctcc agctccttct gccgctccct agacgacctg acggccgcct
      301 cgagctcgtc atcggcgcag ccggccgcgg cggccgcccg aaccgctatc cccgcgacgc
      361 gcctatagtc cgccctggcc ctgccgccgg ccacgaagta gacccggcag ccgcccttgt
      421 acttctccac cctaagaatc ctgaacggct cgagcgatct cgtcgacact acatgggtgc
      481 cgcaacaggc cgagtactcg atgccatcta tctcgatcac acgcaacacc gccgcctcga
      541 ccgacggctc cttgcgtagc gggaaggacg ccggatcctc gggcgggcat aggtgcgtcg
      601 tcaccccata gtcgtcgagg gccgcgcgcg tcacctcgtc ctcgatcgcg tcggcgtcgt
      661 cccttccgaa cggagcgata ttcagatcta tgaaggagtg ccgctcgcct aggtggaacg
      721 acaaggtcct cgcacccatc gcgcgcagga gtatcgacga caggaggtgc tgagccgtat
      781 gttgttcgct atggtcgatc cttcgcccaa ggtctacgac ggaccgaacc gtggcgccgg
      841 ggcagacgcc agccgcggct agcgcgacgg gatcggcgcg taggatatgc gcgacttcgt
      901 cgccctcctc gagcgcggcc tccagcggca gtccggccag gtcgccgagg tcgcagggct
      961 gtcctccgcc ttcgggatag aagatcgtct ccgacagggt cacgacgctg gcgccgtcgg
     1021 cccgctcgct cactccggtt acgaccgagt cgagctccgc gatggccggt cgctcgtagt
     1081 atcgcctgat ggttgccatg gatcgagcat atcctaaaca aggttcgtaa cactagacgc
     1141 gaccacgcgt cgggcggacg cccgctaccg caggccgaaa tagtgccgtt cgagataggc
     1201 gacacgctcg gccgccgctt tccagcgcgg gctcctcggg tactggtcgc gcacacgctt
     1261 atagtactcg tatgatcgct tgatatccct aaacggcgtg tcctgctcgc aggcgagcgc
     1321 gtacaagtag taggcctcgt cgcctccgta cgggaacaat gacaagtagc gatcgagcgc
     1381 ctcgatcgcg gactgcaccc gctttgcgtc gagctcgtcg cgggccagcc tgagcaaagc
     1441 ctctggatcg ctcagcgcgg atgaagcggg agcgggagct tcgggagcgg gagccgcggg
     1501 aacagtcgga gtcgagactc gagacgacgc cgagggagtg cttgttgcag gagcgcttgt
     1561 cgctggagcg gtgggaacgg tagcggtaga tgcggctgat gcggtagccg gaacggaagc
     1621 c
//
LOCUS       DAOOLN010000477         1620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_98200_length_1620_cov_3.279872, whole genome shotgun sequence.
ACCESSION   DAOOLN010000477 DAOOLN010000000
VERSION     DAOOLN010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98200_length_1620_cov_3.279872"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="PLW80_06685"
     CDS             complement(<1..172)
                     /locus_tag="PLW80_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HPB66226.1"
                     /translation="MLEKLTQTFGDIVRTVGGKSTISEKNIEEAVDRIRMALLEADVN
                     LGVVRRFVNATIE"
     gene            complement(261..701)
                     /locus_tag="PLW80_06690"
     CDS             complement(261..701)
                     /locus_tag="PLW80_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66227.1"
                     /translation="MGFLKDFYNGYNASYATGASITDTVQAPVGDPFSLAVTVTKYTR
                     DDAAGFAYEEEATFTEWYGDGSSPYMELTGTVTFTSVNNTDCTIKGALKVANTRDGAD
                     EDDAPDITIDSIAFDLSIDDQNWGTGTVEADGRTFTWTGSTRID"
     gene            complement(676..837)
                     /locus_tag="PLW80_06695"
     CDS             complement(676..837)
                     /locus_tag="PLW80_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66228.1"
                     /translation="MNQQKRPLGLFAATLVIAASFGAGSCELFEDPVTEEMANSGYGQ
                     DRGLSQGFL"
     gene            complement(928..1494)
                     /locus_tag="PLW80_06700"
     CDS             complement(928..1494)
                     /locus_tag="PLW80_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPB66229.1"
                     /translation="MHDRQAALALWRRYNDDDSLLRHALSVEAAMRRFASARGEDPDY
                     WGLVGLLHDIDYQSHPDEHLKHAGAMLAEAGYPAPFIRAVESHGWGLCSDVEPVLEME
                     KVLYAVDELTGFIAACAYVRPSRSVLDLEVKSVKKKWKTVAFAAGVNRDVIEKGAAML
                     GLELDSLIEETIVALRGSAEAVGLKGAL"
BASE COUNT          262 a          531 c          517 g          310 t
ORIGIN      
        1 cctctatcgt cgcgttgacg aagcgcctga cgacgcccag gttgacgtcg gcctcgagca
       61 gggccatccg gatccggtcg acggcctcct cgatgttctt ctcgctgatc gtcgacttgc
      121 cgccgacggt cctgactata tctccgaatg tctgggtaag cttctcgagc atgggtatcc
      181 gttcctgggg gcgtgattcc gggcgcgaaa gggtcgcgtt cggccgggcg atggattatc
      241 gcccggccga acgcctcgcg tcaatcgatc cgggtcgagc ccgtccacgt gaaggttcgc
      301 ccgtcggcct cgaccgtgcc cgtaccccaa ttctgatcgt cgatggacag gtcgaaggcg
      361 atggaatcta tcgtgatatc cggggcgtca tcctcgtccg ctccatccct cgtgttcgcg
      421 accttgagcg cgcctttgat cgtacagtcg gtgttgttga cgctcgtgaa cgtaacggtg
      481 cccgtgagct ccatgtacgg gctagagccg tccccgtacc actcggtgaa ggtcgcctcc
      541 tcttcgtatg cgaagccggc agcgtcgtct ctcgtatact tcgtcaccgt aacggcgagg
      601 gagaacggat ccccgaccgg agcctgcacg gtatccgtga tcgaggcgcc cgtggcgtag
      661 ctcgcgttgt atccgttata gaaatccttg agaaagccca cggtcttggc cgtaaccgct
      721 attggccatc tcctcggtga ccgggtcctc gaacagctcg caggagcccg ccccgaagct
      781 tgcggcaata acgagcgtgg cggcgaacag gcccagcggt cgtttctgtt ggttcatact
      841 tccccctgac actgtacgag tatctagcat caaggcccgc aagtacaccg agcgcccggg
      901 gacgcgaggg taagcgagcc ggccgcatca gagcgcgccc ttcagcccca ccgcctcggc
      961 cgagcccctg agggcgacga tagtctcctc gatgaggctg tccagctcca ggccgagcat
     1021 ggcggcgccc ttctcgatga cgtcccggtt gacgccggcg gcgaaggcga ccgtcttcca
     1081 tttcttcttg accgatttga cctccaggtc gagcacggag cggctcggcc gcacgtaggc
     1141 gcaggcggcg atgaagccgg tcaactcgtc gacggcgtac aggaccttct ccatctcgag
     1201 cacgggctcc acgtcggagc agagacccca gccgtggctc tcgaccgcgc ggatgaaggg
     1261 agccggatag ccggcctcgg cgagcatggc cccggcgtgc ttaaggtgct cgtcgggatg
     1321 ggactggtag tcgatatcgt ggagcaggcc gacgaggccc cagtaatccg gatcctcccc
     1381 gcgggccgag gcgaaacgcc gcatcgcggc ctccaccgat agcgcgtggc gcaggagcga
     1441 gtcgtcgtcg ttgtaacggc gccagagggc gagcgcggct tgccggtcgt gcatcgtgcg
     1501 tcctttccgc ggcccgggcg gggccgtagc gtaatggact atcctatcat atactcggcg
     1561 gcgtgcgaat gaaaccgttc gccgcactgg ccctggtcgt catcgcctcg tcgctggccc
//
LOCUS       DAOOLN010000478         1620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_98244_length_1620_cov_2.279872, whole genome shotgun sequence.
ACCESSION   DAOOLN010000478 DAOOLN010000000
VERSION     DAOOLN010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98244_length_1620_cov_2.279872"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..946
                     /gene="aroF"
                     /locus_tag="PLW80_06705"
     CDS             8..946
                     /gene="aroF"
                     /locus_tag="PLW80_06705"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPB66230.1"
                     /translation="MYPSVAGAVAIGGQGKPNDDYIRYDHGRPSRRKVDIMKDLKLTG
                     VRPDGARTVVEVGGIRIGDGFTVIAGPCAVESEDQLLRTAVAIKEAGATMLRGGAFKP
                     RTSPYAFQGLGLKGLKILDKARRETGLPIVTEVIDTRDVSWVGEYADVLQIGARNMQN
                     FSLLREAGKSGRPILLKRGMYSTLEEWLNCAEYILAEGNPDVILCERGIRSFETYTRN
                     TLDLSIVPSVKATSHLPIIVDPSHGTGVLGLIEPMSLAAVAAGADGLEIEVHIDPPTA
                     LSDKAQQLSPEAFKRLMRKIATMRRTMDELAAEDGR"
     gene            943..>1620
                     /locus_tag="PLW80_06710"
     CDS             943..>1620
                     /locus_tag="PLW80_06710"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HPB66231.1"
                     /translation="MTAPEIRFGPAVGLGSLAEPATTVVVADALTSGLMPPGFDPGRV
                     VIVERGESAKSLSSLEAVYARFLELGVERDWTVVGLGGGSVSDLAGLAASTWMRGVDF
                     GFVPTTLLAMVDASVGGKNGVDFRGYKNLIGCFSQPRFVLVDTSLLDGLPDDELARGF
                     VEAIKHGVIAGGEHYAAVGRAVDRRGRIRRGELGEVVRLSVELKSAFVEADALELGER
                     RKLNLGHS"
BASE COUNT          267 a          547 c          568 g          238 t
ORIGIN      
        1 cccccggttg tatccttccg tcgcgggcgc cgtcgcgatc gggggccagg gcaaaccgaa
       61 cgacgactat attaggtacg atcacggccg gcccagccgg cggaaggtag acatcatgaa
      121 ggatctgaag ctgaccggag tccggcccga cggggcgagg accgtggtcg aggtcggcgg
      181 catacgcata ggcgacggct tcaccgtcat cgcgggacca tgcgccgtgg agtccgagga
      241 ccagctcctg cgcacggccg tagcgatcaa ggaagcggga gcgacgatgc tccgcggcgg
      301 cgccttcaag ccgcgcacct cgccgtacgc gttccaggga ctcggcctca aggggctcaa
      361 gatcctggac aaggcgaggc gcgagacggg cctgcccatc gtcaccgagg tgatagacac
      421 ccgcgacgtc agctgggtcg gcgaatacgc cgacgtgctc cagatcggcg ccaggaacat
      481 gcagaatttc tcgctgctgc gcgaggcggg caagtccggc cggccgatac tcctcaagcg
      541 cggcatgtac tcgaccctcg aggaatggct taactgcgcc gagtacatcc tggccgaggg
      601 caacccggac gtcatcctct gcgaacgggg catccgcagc ttcgaaacct atacgcgcaa
      661 taccctcgac ctgtccatcg ttccgtcggt caaggcgacg agccatctgc cgatcatagt
      721 cgacccgtcg cacggcaccg gcgtgctcgg cctgatcgaa ccgatgagcc tggccgccgt
      781 cgccgcgggg gccgacgggc tcgagataga agtgcatatc gatccgccca cggccctgag
      841 cgataaggct cagcagctgt cgcccgaggc cttcaagcgg ctgatgcgca agatagcgac
      901 gatgcggcgg acgatggacg agctggccgc ggaggacggg cgatgaccgc gccggagata
      961 cgcttcggcc ccgccgtcgg cctcggctcg ctcgccgagc cggcgacgac ggtcgtcgtc
     1021 gcggacgccc tgacctccgg cctcatgccg cccggcttcg accccgggcg cgtcgtgatc
     1081 gtggagcgcg gcgagtcggc caagagccta tcgtcgctcg aggccgtgta cgcgcgcttc
     1141 ctggagctgg gcgtcgagcg cgactggacc gtggtcgggc tcggcggcgg cagcgtctcg
     1201 gacctggcgg gcctggccgc gtccacctgg atgcgcggcg tcgacttcgg tttcgtgccg
     1261 acgacgctgc tcgcgatggt cgacgcctcg gtcggcggca agaacggcgt cgacttccgc
     1321 ggttacaaga acctgatagg ctgcttctcc cagccgcgct tcgtgctcgt cgacacgagc
     1381 ctgctcgacg ggctgcctga cgacgagctg gcccgcggct tcgtcgaggc catcaagcac
     1441 ggcgtcatcg caggcggcga gcactacgcc gccgtggggc gggcggtcga ccgacgcggc
     1501 cggatcaggc gcggggagct cggcgaggtc gtcaggctgt cggtcgagct caagtcggcc
     1561 ttcgtcgaag ccgacgcgct cgagctcggc gagcggcgca agctgaacct cggacacagc
//
LOCUS       DAOOLN010000479         1619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_98290_length_1619_cov_3.947570, whole genome shotgun sequence.
ACCESSION   DAOOLN010000479 DAOOLN010000000
VERSION     DAOOLN010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98290_length_1619_cov_3.947570"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..1444)
                     /locus_tag="PLW80_06715"
     CDS             complement(26..1444)
                     /locus_tag="PLW80_06715"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HPB66232.1"
                     /translation="MIRAHHSPDDPGGEGSGLSFSADVRKNDGNIEITVSADMMTATA
                     TLYPPIGDGAPLTPDYAAELLARMGIHSGLLWDELSERIFDVNADRRVIRDVVVARGS
                     PPVSEHPEHIVVDDRFLPGFRPVSDEEHTVDWKAISQVLVVKQGEGIGRVIPRRDGAN
                     GCDVTGNPIAFGKDQVTTYALGKNVERDGDDIRAVCDGRVTLDGQRISVEEVLVIKGD
                     VDYRVGHIMFPGDVVIEGGVAAGFKVYSGGSISIKETMDAFDVSAKRDLICAQGIIGK
                     DLGQVRVGGSLKAKFIENARVAVRGDVEVPGSIVGSSIYTLGRLAMGDKGRIVGGEAF
                     ATHGVSCGFLGGGTKPVTIVNVGIDFTIQQKLEQANAALRALTVRLARLQNVRKLRPD
                     DEPLAKAVEEARAKLQSLTDNVADLTRRVDIDEGASVEVKGIVYPGVIVTICHIRVNV
                     DEPLKKARFRLDRAANKIIIER"
BASE COUNT          273 a          510 c          522 g          314 t
ORIGIN      
        1 cgaattttat tgagcggcga gccggctagc gctcgatgat gatcttgttg gccgcccggt
       61 ccagcctgaa cctggccttc ttgaggggtt cgtcgacgtt gaccctgata tggcagatgg
      121 tgacgatgac gccggggtag acgatgccct tgacctcgac cgaggcgcct tcgtctatgt
      181 cgacgcgcct ggtcaggtcg gccacgttgt cggtcagcga ttgaagcttg gcccgagcct
      241 cctcgacggc cttagccagg ggctcgtcgt caggccgtag cttacggacg ttctgcaacc
      301 tcgcgaggcg tacggtcagc gcgcgcagcg cggcgttcgc ctgctccagc ttctgctgga
      361 tggtgaagtc tatgccgacg ttgactatgg tgactggctt cgtgccgccg cctaggaagc
      421 cgcagctgac gccgtgggtg gcgaacgcct cgccgcctac gatgcggccc ttatcgccca
      481 tagccaggcg cccgagcgta tagatcgagg agccgacgat ggagcctggc acctccacgt
      541 cgccgcgtac cgccacgcgt gcgttctcga tgaacttggc cttgagcgag ccgccgaccc
      601 tgacctggcc caggtccttg ccgatgatgc cctgggcgca gatcaggtcg cgcttggcgc
      661 tgacatcgaa cgcgtccatc gtctccttta tggagatcga gccgcccgag tagaccttga
      721 agccggccgc gacgccaccc tcgattacaa cgtcgccggg gaacatgatg tggccgacgc
      781 gatagtcgac gtcgcccttg atgacgagca cctcctcgac gctgatgcgc tggccgtcca
      841 gggtcacgcg gccatcacat acggcgcgga tatcgtcgcc atcgcgctcg acgttcttgc
      901 cgagcgcgta cgtggttacc tggtccttgc cgaaggctat cggattgccg gtcacgtcgc
      961 aaccgttagc gccgtcgcgg cggggaatga cgcggcctat gccttctcct tgcttgacga
     1021 cgaggacctg gctgatagcc ttccagtcga cggtatgctc ctcgtcggac acgggcctga
     1081 agccgggcag gaagcgatcg tcgaccacga tatgctccgg gtgttccgac accgggggcg
     1141 agcccctggc cacgacgacg tcgcggatga cgcggcgatc ggcattgacg tcgaatatac
     1201 gctcggacag ctcgtcccag agcaggccgg aatgtatgcc catgcgcgcc agtagctcgg
     1261 cggcgtagtc tggagtcagg ggcgccccat cgccgatggg aggatacagg gtggcggtgg
     1321 cggtcatcat gtcggccgaa accgttatct cgatgttgcc gtcgttcttg cgcacgtcgg
     1381 ccgagaacga gagtccggaa ccctctccgc cgggatcgtc ggggctatga tgggcgcgaa
     1441 tcacgatggc gttcctccct cgttatacta tcggtacgga atcgctccgg cttgccaaaa
     1501 aaccgccgtc gcggcatact gtcgtatcgt atgcgcgacg cttcatcgat tgcgaaaagg
     1561 cgggaacgcc tcaaggtgat cctcaagctc ctcagggaga acagggtcga gtcccagga
//
LOCUS       DAOOLN010000480         1619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_98324_length_1619_cov_2.850384, whole genome shotgun sequence.
ACCESSION   DAOOLN010000480 DAOOLN010000000
VERSION     DAOOLN010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98324_length_1619_cov_2.850384"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PLW80_06720"
     CDS             <1..471
                     /locus_tag="PLW80_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPB66233.1"
                     /translation="IIGGGGKSGVLCCVQAMKNVGPYGKVIVVEYSREHATRIERMGL
                     ATHVIVADATDALDVYRKVTDITGPRGCDVTVNNVNVPNTEMTSILITKGQGLIYFFS
                     MATSFTKAALGAEGVGKDIELIVGNGYTKGHSDLALDILRESADIRALFEKLYV"
     gene            complement(462..1334)
                     /locus_tag="PLW80_06725"
     CDS             complement(462..1334)
                     /locus_tag="PLW80_06725"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPB66234.1"
                     /translation="MPETYTIASFNAENFSMLLDADYGAAEFHALSDERYAAMNPSIF
                     NPNKDRAKASAIASTILERDFDVVGLCEIGGMETLENFNRYYLGGRYECFLHEANSRR
                     GIFVGALVKRGRFDRIEAKPVDGPFSRNVLELRLASGDDRFRLIVVHLKSQSGQDRGI
                     PQRVAEVRRLVEVAAPPACVVMGDFNGILIRGEAEFEYEPFLELPFRDVLEATGVPPG
                     ARFTHFYFRGAEPSFSQLDYIFCSDDVEVADGGVLAELVPLNYEQRRRLPSDHAFIWA
                     AIRPWPVVAPDGQT"
     gene            1448..>1619
                     /locus_tag="PLW80_06730"
     CDS             1448..>1619
                     /locus_tag="PLW80_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HPB66235.1"
                     /translation="MERSIEEVCEAILRIELVPALGCTEPIAIAYAAAVARETLGVFP
                     ERMTIRCSGNVVK"
BASE COUNT          299 a          504 c          543 g          273 t
ORIGIN      
        1 atcatcggcg gaggaggcaa gtccggcgtg ctgtgctgtg tgcaggccat gaagaacgtc
       61 gggccctacg gcaaggtcat cgtcgtcgag tactccaggg agcacgccac caggatagag
      121 cggatgggcc tggccacgca cgtgatcgta gccgacgcga ccgacgccct ggacgtgtac
      181 cgcaaggtga ccgacatcac cggtccccga ggctgcgacg tgacggtcaa caacgtcaac
      241 gtgccgaaca cggagatgac gagcatcctg atcaccaagg gccagggcct catctacttc
      301 ttctcgatgg ccacctcgtt caccaaggcg gccctcggcg cagagggcgt gggcaaggac
      361 atcgagctga tcgtcggcaa cgggtacacc aaggggcact cggacctggc cctcgatatc
      421 ctgcgcgagt cggcagatat cagggcgctg ttcgaaaagc tctacgtctg accgtccggg
      481 gcgacgacgg gccacggtcg tatcgccgcc cagatgaagg cgtgatcgga cggcaggcgc
      541 cgtcgctgct cgtagttgag cggcacgagc tccgcgagga cgccgccatc agccacctcg
      601 acgtcgtccg agcagaagat atagtcgagc tggctgaagc tcggttcggc tccccggaag
      661 taaaaatggg tgaagcgggc gcccggcggt acgcccgtcg cctcgagcac gtcgcggaac
      721 ggtagctcca ggaacggctc gtactcgaac tccgcctcgc cgcgtatcag gatgccgttg
      781 aagtcgccca ttacgacgca ggcgggcggg gcggcgacct cgacgagcct gcggacctcg
      841 gcgacgcgct ggggtatgcc gcggtcctgc cccgactgcg acttgaggtg caccacgata
      901 aggcggaacc tgtcgtcgcc ggaggccagg cgcagttcga gcacattgcg cgagaagggg
      961 ccgtcgacgg gcttcgcctc gatgcggtcg aagcggccgc gcttgaccag ggctcccacg
     1021 aagatgccgc gacggctgtt ggcctcgtgc aagaagcact cgtagcgccc gccgaggtaa
     1081 tagcggttga aattctcgag cgtctccatg ccgcctattt cgcatagccc gaccacgtcg
     1141 aagtctcgtt ccaggatcgt ggaggctatc gccgacgctt tggcccggtc cttgttcggg
     1201 ttgaagatcg acgggttcat cgcggcgtag cgctcgtccg agagggcgtg gaactcggcg
     1261 gcaccgtagt cggcgtccag tagcatcgag aaattctccg cattgaacga cgctatcgtg
     1321 taggtctcgg gcactgggcc tcctggttcg ggctatcggg ccatcctata ccgtaacgcc
     1381 cgcggcggac gatagcgcct tgcggccggc gcctggaatc gctatcctgt cgccaatagg
     1441 agtcttcatg gaacggagca tcgaggaggt ctgcgaggcc atcctgagga tagaactggt
     1501 gccggctctc ggctgcaccg agccgatagc tatagcctac gccgcggccg tggctcgcga
     1561 gacgctcggc gttttcccgg agcgcatgac gatacgctgt tcgggcaacg tggtcaaga
//
LOCUS       DAOOLN010000481         1618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_98418_length_1618_cov_3.001280, whole genome shotgun sequence.
ACCESSION   DAOOLN010000481 DAOOLN010000000
VERSION     DAOOLN010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98418_length_1618_cov_3.001280"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="PLW80_06735"
     CDS             complement(<1..820)
                     /locus_tag="PLW80_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66236.1"
                     /translation="MLNEIRIAYIDSGINQILADIPESAVNAPYAMRFSQGEEYFIEL
                     GSPLIVPHLPIHHDIRTPVPAADYAAAIRDVVSQLTAVLPECFSGLTYFFDPAEILKP
                     CFYRLYKVEADVYLYLLRVDLLPKPFDADTIEPGTNDRTSAYSTRRVYLESELIPLEA
                     VMWEAGRVKAFRIKQLISQTWIGETGKGYLVRGVWMDSDLSKFFTKLFLPSGKRVYPF
                     YPLFCKYKTLCCMPPLLSADGRRSLIPLLHKAIQFLAPEIGRIQEVLRNAKFDES"
     gene            951..>1618
                     /locus_tag="PLW80_06740"
     CDS             951..>1618
                     /locus_tag="PLW80_06740"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HPB66237.1"
                     /translation="MNAKARILTLALIIAMTGLAAWAQGAPAAVVEYSSGDDVLVIRA
                     GRRLGIADPIGLELYEGDQVQTGKGVFVEMRLVSGGAVVKLAENTTFVLERLSDGQTS
                     LQLVYGRVRAKVERLAGTDTFSVRSSQAIAGVRGTDFGLDVVASAGGAATTRTNAYCF
                     VGAVEVTAFIRSDAHAAESLEAIPRSYVVAAGEMLSVESRSGKAAATKTALDETIRGF
                     WAEY"
BASE COUNT          292 a          452 c          557 g          317 t
ORIGIN      
        1 gcgattcgtc gaacttggcg ttcctgagga cttcctgtat ccggcctatc tcgggggcca
       61 ggaactggat agccttatgg agcagcggga tgaggctccg cctaccgtcg gcgctgagca
      121 ggggcggcat gcagcagagc gtcttgtact tgcagaacag ggggtagaat ggataaacgc
      181 gcttgccgga cggcaggaac agcttcgtga agaacttcga caggtcactg tccatccaga
      241 cgcccctgac gaggtagccc ttgccggtct cacctatcca ggtctgcgag atcagctgct
      301 tgatcctgaa ggccttgact ctgccggcct cccacatgac cgcctcgagc gggatcagct
      361 ccgactcgag gtagactcgc cgcgtcgagt aggcggaagt ccggtcgttg gttccgggct
      421 cgatggtatc ggcgtcgaac ggcttgggga ggaggtcaac cctgagcagg tagaggtaga
      481 cgtcggcctc gactttgtag agtcggtaga agcatggctt caggatctcg gccgggtcga
      541 agaagtaggt aaggcccgag aagcattcgg gcaaaacggc cgtgagctgg ctgaccacgt
      601 cgcgtatcgc cgcggcatag tcggcggccg ggacgggcgt gcgtatgtcg tggtggatcg
      661 gcagatgggg tacgatcagg ggcgatccga gctcgatgaa gtattcctca ccctggctga
      721 atcgcatcgc gtacggagca ttgaccgcgc tttccggtat gtcggcgagt atttggttaa
      781 taccagaatc tatgtaggcg atcctgattt cattgagcaa ctgctccata ttttggacaa
      841 gtatagccgc tgaaagcgga taaatcaacg aggccgtggt cggcgttgcg actgggtccc
      901 ggccgtggta tagtcgagcc gttgcatcct ctcgggtcgg aggtggatcg atgaatgcta
      961 aggcgaggat ccttacgctg gcgctgatca ttgcgatgac gggactggcc gcctgggcgc
     1021 agggtgcgcc cgcggcagtg gtcgagtact cgagcgggga cgacgtgctg gtcatcaggg
     1081 ccggacgtag gctcggcatc gccgatccta tcggcttgga gctgtacgaa ggcgaccagg
     1141 tccagaccgg caagggtgtt ttcgtggaga tgcgcctcgt ctcgggcggc gccgtcgtca
     1201 agctggccga gaataccacc ttcgtgctcg agcgcctgtc ggacggccag acctcgctgc
     1261 agctcgtcta cggccgggtt cgtgccaagg tcgagcggct ggccggtacg gataccttct
     1321 cggtacgcag ctcccaagcg atcgccggag tacgcggcac cgatttcggc cttgacgtcg
     1381 tggcctccgc cggcggcgcc gcaaccacga ggacgaatgc ctattgcttc gtgggagcgg
     1441 tcgaggtgac ggccttcata cgctccgacg ctcacgccgc cgaatcgctc gaggctatcc
     1501 ctcgctccta tgtggtggcg gccggggaga tgctgtcggt ggagagtcgt tccggcaagg
     1561 cagcggctac gaagacggcg ctggacgaga ccatacgcgg cttctgggcc gagtacga
//
LOCUS       DAOOLN010000482         1617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_98493_length_1617_cov_4.023688, whole genome shotgun sequence.
ACCESSION   DAOOLN010000482 DAOOLN010000000
VERSION     DAOOLN010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98493_length_1617_cov_4.023688"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(358..738)
                     /gene="rplS"
                     /locus_tag="PLW80_06745"
     CDS             complement(358..738)
                     /gene="rplS"
                     /locus_tag="PLW80_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPB66238.1"
                     /translation="MNLIKKIEAEQTKAYQDVKAGDTVRVHFKIVEGKTERIQIYEGL
                     VIAMKNAGIGQTLTVRKNSYGVGVERVFPMHSPRIESIEIVRYGKVRRAKLYYIREKI
                     GKKAKIKEQLGGRKPPVRSQAVQG"
     gene            complement(735..1466)
                     /gene="trmD"
                     /locus_tag="PLW80_06750"
     CDS             complement(735..1466)
                     /gene="trmD"
                     /locus_tag="PLW80_06750"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPB66239.1"
                     /translation="MRLGVLTLFPGILEGFFTSSIMARAVERGLVDYSLTDIREFATD
                     KHHKCDDELFGGGAGMLMMPGPVSAALESVGARESRVVYVTPGGRLFNQAYARELASE
                     EKLVILCGRYEGVDQRVIDRYVTDEISIGDYVLSSGETAALVIVDAIYRLIPGVITGE
                     SLDEESFEGGLLEYPQYTRPAVYDTMVVPDVLVSGHHANIVKWRMKKRLEKTLLYRPD
                     LLQGKGLEGELRAMILELIGQGGEA"
BASE COUNT          281 a          503 c          514 g          319 t
ORIGIN      
        1 cctgcttggt tctagcctga gtccctctcc catcagcgcg gatatagccg ccgccgacag
       61 tgggtcggcg ggaacgcgta gcgaggcgca tagatcggcg gtcttggcta cccggccatc
      121 atgaagagcg ctggaggccg gcgctatggg cggagcgagg gtcggtgcgg cgcgaggcca
      181 cggcgacgat ggttttagcg ccgtcgcccc ctgagcggag cgaggcacgt cggcctgggc
      241 gattctcggg acggtttttc ttaaaaaacg aacgtcggcc gatgacgcat cgccatcgcc
      301 gacgttcgtt gtatgcggag cggtcgacct gtcgttagga ccgaccggtc gcaaggatca
      361 gccctggacc gcctgggagc ggacgggagg cttgcggccg ccaagctgct ccttgatctt
      421 ggctttcttg ccgatcttct cgcggatgta gtacagcttg gcgcggcgga ccttgccgta
      481 gcgcacgatt tcgatgctct cgatgcgcgg cgagtgcatg gggaacacgc gctcgacgcc
      541 gacgccgtat gagttcttgc gtacggtcag cgtctggccg atgccggcgt tcttcatggc
      601 gatcacgagc ccttcgtaga tctggatacg ctcggtcttg ccctcgacga tcttgaagtg
      661 cacgcggacc gtatcgccgg ccttgacgtc ctgataggcc ttggtctgct cggcctcgat
      721 cttctttatg aggttcatgc ctctccccct tgtccaatta attccagtat catcgccctg
      781 agttcaccct cgagtccctt gccctggagc aggtcgggac ggtacaagag agtcttctcg
      841 agccgtttct tcatgcgcca cttgacgatg ttggcatggt ggcccgagac cagaacgtca
      901 gggactacca tggtatcata aaccgccggg cgtgtatact gggggtactc gagcaaaccg
      961 ccctcgaagc tctcctcgtc gagcgactcg ccggtgatga ctcccggaat gagcctgtag
     1021 atcgcgtcga cgatgacgag ggcggcggtc tcccccgacg acagcacgta atcgccgatg
     1081 cttatctcgt cggtcacgta gcggtctata acgcgctggt cgacaccctc gtaacggccg
     1141 cagagtatga cgagcttctc ctcggaagcc agctcgcgag cgtaggcctg attgaacaag
     1201 cgaccgcccg gagtcacata cacgacccgc gactcacgag cgccgaccga ttcgagcgcg
     1261 gcggagaccg gcccgggcat catcagcatg ccggcgccgc cgccgaacag ctcgtcgtcg
     1321 catttatggt gcttatcggt cgcgaactcg cggatgtcgg tcaaggagta gtcgacgagg
     1381 ccacgctcga cggcgcgggc catgatcgag ctcgtgaaga agccttcgag gatgccggga
     1441 aatagcgtca ggacaccgag cctcatccgc gaccttgcct tactcgagta tccatggggc
     1501 taccagctct attcgcctgg cgtggacgtc gacctcgccg acgaattcct tgcggaacgg
     1561 cacgtaggcc gaggagccgt cgggcttggc gacctcgagc aggtcgccgc cgccgga
//
LOCUS       DAOOLN010000483         1617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_98550_length_1617_cov_2.286172, whole genome shotgun sequence.
ACCESSION   DAOOLN010000483 DAOOLN010000000
VERSION     DAOOLN010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98550_length_1617_cov_2.286172"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PLW80_06755"
     CDS             complement(<1..435)
                     /locus_tag="PLW80_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB66240.1"
                     /translation="MTNKSDERGSTGLDGRTALVIGATGGIGAAVAESLAAAGAALTI
                     HGRDDGRLAAKASELAAYGNAVATIRADLSGGVAPPDLLRAAGSCDLLVVAYGPFVYK
                     PLAETGQADWSLAALGCLALPGTIATEAAVGMAGRGFGRILLF"
     gene            501..1289
                     /locus_tag="PLW80_06760"
     CDS             501..1289
                     /locus_tag="PLW80_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPB66241.1"
                     /translation="MRGEPAFDRLAVVLCRVEGSMNVGAACRAMKAMGVRRLVPAACP
                     DHDQTEVRTHALGAYDLYEAAERFDDLGEALAGFSLAAGLTRRVGQRRKESAALPDFA
                     ARLAARLVAAEGRAGQASSAAIVFGNERDGLSDAELALCDEAVRIDTSDLFPSLNLSH
                     AVQIACWELREAVLATSSREGPGTDRPGRTAAPRSSAARAAARMADAMQAAGLYKLAG
                     RPDAEAFLREVAARAALSETELERLSSLFIRLGALGSLDRGPGR"
     gene            1337..>1617
                     /locus_tag="PLW80_06765"
     CDS             1337..>1617
                     /locus_tag="PLW80_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66242.1"
                     /translation="MKRMMLATAIAALALTVAAAQSAPAGEPSYVGRIELGVSIAGLS
                     ALAETGDEAALAALSDRAILLLGTLSAPSVAVDEPFEAVVEFMQGEWRDP"
BASE COUNT          233 a          564 c          592 g          228 t
ORIGIN      
        1 gaagagcagg atgcgcccga aaccgcgccc ggccatcccg acggcggcct ccgtcgctat
       61 cgtccccggc aaggccaggc acccgagcgc ggcgaggctc caatccgcct ggccggtctc
      121 cgcgaggggc ttgtagacga aggggccgta ggcgacgacg aggaggtcgc acgagccggc
      181 ggcacgcagg aggtccggcg gggccacgcc gccggacagg tcggcccgga tcgtagcgac
      241 cgcgttcccg tacgcggcca gctcggaggc cttggccgcc aggcggccgt cgtcccggcc
      301 gtggatggtc agcgcggcgc ccgccgccgc cagggactcg gcgaccgccg cccctatgcc
      361 gccggtcgcg ccgatcacca gcgcggtcct tccgtcgagg ccggtcgagc cccgctcgtc
      421 gctcttgttc gtcatcagag cttgaactat agtccgatcg gcgccccgcg taaacaacgg
      481 ggaggctcga tggaggcccg atgaggggag aaccggcgtt cgaccggctg gcggtggtgc
      541 tatgccgcgt cgaaggctcg atgaacgtag gggcggcatg ccgtgcgatg aaggcgatgg
      601 gcgtgcgccg actagtgccg gcggcctgcc ccgatcacga tcagacggag gtgcgaacgc
      661 acgcgctcgg agcctatgac ctctacgagg cggccgagcg attcgacgat ttaggcgagg
      721 ccctcgccgg tttctcgctg gccgccggct tgacgaggcg ggtcggacag cggcgtaagg
      781 agagtgcggc gctacccgat ttcgcggcca gactcgcggc gcgcctcgtc gccgccgagg
      841 ggcgggccgg acaggcaagc tcggccgcca tcgtattcgg aaacgagcgc gacgggctat
      901 ccgacgccga gctggccctc tgcgacgagg ccgtgcgcat cgatacctcg gacctcttcc
      961 cctccctgaa cctgtcccac gccgtgcaga tcgcctgctg ggagctacgc gaggccgtcc
     1021 tggccacctc gtcgcgcgag ggtccaggga ccgaccggcc cggcagaacg gcggcgccac
     1081 gctcgtccgc cgcgcgggcg gccgccagga tggccgacgc tatgcaggcg gccggcctgt
     1141 acaagctggc cggccggccc gacgccgagg ctttcctgcg ggaggtcgcg gctcgggcgg
     1201 ccctttcgga gacggagctg gaacgcctat cgtcgctatt catccggctc ggcgccttag
     1261 gctcgcttga ccgcggcccc gggcgatagt aggcttctcg tatacgggcg gggatcaccg
     1321 cgcgacggag ggcgaaatga aacgaatgat gctcgctacg gcgatcgccg ccttggccct
     1381 gacggttgcc gccgcgcagt cggctccggc tggcgagcct agctacgttg gcaggatcga
     1441 actcggcgtc agcatcgccg gcctctcggc cttggcggag acgggcgacg aggcggcctt
     1501 ggcggccctg tccgaccgcg ccatcctgct cctcggcacg ctatcggcgc cgagcgtcgc
     1561 cgtcgacgag cccttcgagg cagtggtcga gttcatgcaa ggcgagtgga gggatcc
//
LOCUS       DAOOLN010000484         1616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_98622_length_1616_cov_2.804612, whole genome shotgun sequence.
ACCESSION   DAOOLN010000484 DAOOLN010000000
VERSION     DAOOLN010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98622_length_1616_cov_2.804612"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PLW80_06770"
     CDS             complement(<1..581)
                     /locus_tag="PLW80_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB66243.1"
                     /translation="MANRKLGDARKGDALLVPIVAVLLGFVVGSVVVMITGKSPLVMF
                     NAMLQGLTGLNVARGSFNPRYIGELVIQAMPIILTALCFGFAARTGLFNIGAEGQLMI
                     GSLAATTVALLVKAPAFIHIPLVLVAGMAAGALWGAIPGYLKARFNVHEVVVTIMLNY
                     VALHMNNLMILNVFGSVDRVKTAQFPQSALLKDP"
     gene            complement(583..>1616)
                     /locus_tag="PLW80_06775"
     CDS             complement(583..>1616)
                     /locus_tag="PLW80_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB66244.1"
                     /translation="FDEPTAVLTPQEIHELMGIMRRLVEEGKTILLITHKLKEIKAVA
                     DTCTVLRRGKLIGTVPVAGTSEASMAEMMVGREVSFSVDKGPSHPGEVVLDIKDLCVK
                     NAKGLLGVKNLSLSVREGEILGLCGIDGNGQTELIQALTGLAPIESGSVTVRGVDITD
                     LPVKQRLDSGLGHIPEDRHRHGLVLDFRLDENMVIHSYGSEPFSRHGILDYGAIRKNA
                     ERLIDEFDVRSGRGADTKARSMSGGNQQKAILAREIDRSPIAFVIAQPTRGLDVGAIE
                     YIHKRIVAERDKGKAILLASLELDEILDVSDRIAVIYGGEIVGVVDADKTDENELGLM
                     MSGSVRKAG"
BASE COUNT          282 a          515 c          518 g          301 t
ORIGIN      
        1 ggatccttga gcagcgccga ctgcgggaac tgggccgtct tgaccctgtc caccgagccg
       61 aacacgttga ggatcatgag gttattcata tgcagcgcga cgtaattgag catgatggtc
      121 acgacgacct cgtgcacgtt gaaacgcgcc ttcaggtaac ccgggatcgc tccccacagc
      181 gcgcccgctg ccatgccggc gacgagcacg aggggaatat ggatgaaggc cggcgccttg
      241 accaacagcg ccacggtggt agccgccagc gagccgatca tcagctggcc ctcggcgccg
      301 atattgaaca ggccggtacg ggcagcgaag ccgaagcaca gggccgtcag gatgataggc
      361 atggcctgga tgaccagctc gcctatgtag cgcgggttga acgatcccct ggcgacgttc
      421 aggccggtca ggccctgtag catcgcgttg aacatgacga gggggctctt gccggtgatc
      481 atgacgacca ccgaaccgac cacgaagccg agcagcaccg cgacgatcgg tactaagagc
      541 gcgtcgccct tcctcgcgtc gcctagtttt ctgtttgcca tgttaaccgg ccttcctgac
      601 cgagccggac atcatcaggc cgagctcgtt ctcgtcggtc ttgtcggcgt cgaccacgcc
      661 gacgatctcg ccgccgtaga tgaccgcgat cctgtcggac acgtcgagta tctcgtcaag
      721 ctcgagcgac gcgagcagga tcgccttgcc cttgtcgcgc tcggcgacga tgcgcttatg
      781 gatgtattcg atagcgccca cgtcgaggcc gcgcgtcggc tgcgctatga cgaaggcgat
      841 gggcgaccgg tcgatctcgc gcgcgagtat cgccttctgc tgattgccgc ccgacatcga
      901 gcgggccttg gtatcggcgc cgcgcccgga gcgcacgtcg aactcgtcga tcaggcgttc
      961 ggcgttcttg cgtatggcgc cgtagtccag gatgccgtgc ctcgagaacg gctcggagcc
     1021 ataggaatgg atgaccatgt tctcgtcgag cctgaagtcg agcacgagac cgtggcgatg
     1081 cctatcctcg gggatatggc cgagcccgct gtcgagccgc tgcttgaccg gcaggtcggt
     1141 gatgtcgacg ccccggaccg tcacggagcc tgactcgatg ggcgcaaggc cggtcagggc
     1201 ctggatcagt tcggtctggc cgttgccatc gatgccgcac aggccgagaa tctcgccctc
     1261 gcgcacgctc agcgacaggt tcttgacgcc caggaggccc ttggcgttct tgacgcatag
     1321 atccttgata tcgaggacga cctcgcccgg atgggagggg cctttgtcga ccgagaagct
     1381 gacttcgcgc ccgaccatca tctccgccat gctcgcctcg ctggtcccgg cgaccggcac
     1441 ggtaccgatc agcttgccgc gcctgagcac ggtgcaggta tcggcgaccg ccttgatctc
     1501 cttgagcttg tgggtgatca ggaggatggt cttgccctcc tcgacgagcc gacgcatgat
     1561 gcccatcagc tcgtggatct cctgcggcgt cagtaccgcg gtaggctcgt cgaaga
//
LOCUS       DAOOLN010000485         1615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_98718_length_1615_cov_2.760897, whole genome shotgun sequence.
ACCESSION   DAOOLN010000485 DAOOLN010000000
VERSION     DAOOLN010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98718_length_1615_cov_2.760897"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="PLW80_06780"
     CDS             <1..906
                     /locus_tag="PLW80_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HPB66245.1"
                     /translation="GCDRYYTEMTSAAGYLANSPYDRFFVDSEPRPERTAVQFYGAEV
                     EPIAAAAARLLGERTAAGSCLGGLDANFGCSAPLIEKAGGGVSWMKDADRSARLIAAL
                     KTAAPGVPVSAKLRLGYEASEAALIAFCRALEAAGADYIVVHPRLKHEKFRRSGHWEF
                     VAAVSTAVGIPVIGNGDIRDAGGYRTVMERYGATGAMLGREAVRRPWIFSLIRGKEAN
                     PAFGMSVAIEETGLRMIALIRELLPSPLHISRARRFFFYYCDNLSFGHHLRWSIQNAD
                     GLDEVERLFRAYFDEVPSDRVRLER"
     gene            997..1563
                     /locus_tag="PLW80_06785"
     CDS             997..1563
                     /locus_tag="PLW80_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB66246.1"
                     /translation="MAETLATMFMERVKARPELVMQYSKDGDGRFQPTSYAELLEHVA
                     TFAAGLLELGLRRGDQIGLISDNRREWLISDLAIIGLGAADVPRGCDATDQELAYILS
                     WSECAVAIVENDKQLAKLVARKREMPLLSTVVSFDPPSDGAAAVFLSLPLQFLHLSPH
                     CRHLLLEFLLLVEHPFLWRHIWVQWPRL"
BASE COUNT          273 a          517 c          526 g          299 t
ORIGIN      
        1 ggatgcgatc gatactacac cgagatgacc agcgccgcgg gttacctggc caactcccct
       61 tacgaccgct tcttcgtcga ttcggagccg cgtcccgaac gtaccgccgt ccagttctat
      121 ggcgccgagg tcgagccgat cgccgcggcc gccgcgcggc tcctgggcga gaggacggcc
      181 gccggctcgt gcctcggcgg cctggacgcc aacttcgggt gctcggcccc tctcatagag
      241 aaggccggcg gtggggtaag ctggatgaag gacgcagacc ggtcggcccg cctgatcgcg
      301 gccctcaaga ccgccgctcc gggcgttccg gtctcggcca agctacggct cggttacgag
      361 gcctccgaag cggcgctgat agccttctgc cgggcgctcg aagccgccgg cgccgactac
      421 atcgtcgtcc accccaggct caagcacgaa aagttcaggc gttccgggca ctgggagttc
      481 gtggccgccg tctcgacggc cgtcggcata ccagtgatag gcaacggcga catccgcgat
      541 gcgggcgggt ataggaccgt catggagcgc tatggagcca cgggcgctat gctcggccga
      601 gaggccgtcc gtcggccctg gatattctcg ctcatccgcg gtaaagaagc gaatccggcc
      661 ttcggcatga gcgtggccat cgaggagacg ggcctgagga tgatagcctt gatccgggag
      721 ctgctaccct cgccgctcca tatcagccgg gccaggcgtt tcttcttcta ctattgcgac
      781 aacctgtcgt tcggccatca cctccgctgg tccatccaga acgccgacgg tctggacgag
      841 gtcgagcgcc tgttcagggc ctacttcgac gaggtgccgt cggatcgcgt caggctggag
      901 cgctgagcgt agaaggtttt cgccgacact tttcaaccct tgcgtctttt tacacttttc
      961 agtatattgt caaaagaagc tgaagaggag gctaccatgg ccgagacgct cgcgacaatg
     1021 ttcatggaac gggtcaaggc gagacccgag ctcgtgatgc agtacagcaa ggacggcgac
     1081 ggccgcttcc agccgacaag ctacgcggaa ctgctcgagc acgtggcgac ctttgctgcc
     1141 gggctgctcg aactgggcct gaggcgaggc gaccagatag gactcatctc cgacaaccgc
     1201 cgcgagtggc tgatcagcga cctagccatc atcgggctcg gcgcggccga tgtaccgcgc
     1261 ggttgcgacg cgaccgacca ggagctggcc tacatcctgt cgtggagcga gtgcgccgtg
     1321 gccatcgtgg agaacgataa gcagctggcc aagctggtgg ccaggaagcg ggaaatgccg
     1381 ctcctgtcga cggtcgtatc gttcgaccct ccgtcggatg gcgccgctgc ggtcttcctc
     1441 agcctgccgc tgcagttcct ccatctgtcg ccgcattgcc gccatctgct gctggaattc
     1501 ctgttgctgg tggagcaccc gttcctgtgg cgtcatattt gggtccagtg gcctcgcttg
     1561 tgacgatgtg ccgaaagccg ttgcgttgga tcggcgcggg acggcgcgga cccga
//
LOCUS       DAOOLN010000486         1615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_98734_length_1615_cov_2.438462, whole genome shotgun sequence.
ACCESSION   DAOOLN010000486 DAOOLN010000000
VERSION     DAOOLN010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98734_length_1615_cov_2.438462"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PLW80_06790"
     CDS             complement(<1..494)
                     /locus_tag="PLW80_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66247.1"
                     /translation="MTDARDEWLRERERKRAAVAVSSALGAYLLLFGVAVLLSLFAVQ
                     DMADFSGPIIVRLGSPDGVDAPHPVEAERSSAQAESVPEAPAAALPEPPAPAAPSPLP
                     TAKPAAAKPTPTTQPAPAPKPAAPPAPEPVVIRGSESGNSYDMTFLSGSGVVGRSLYL
                     PIWL"
     gene            complement(491..910)
                     /locus_tag="PLW80_06795"
     CDS             complement(491..910)
                     /locus_tag="PLW80_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPB66248.1"
                     /translation="MTIERRLKPQINIDLTPLIDIIFQLVIFFMISSTFKTAPGIELR
                     LPDSGTATAITITELSVVAVSEDEVYVNKTRTTAEGAESVIKLELEGKQVDEVQAVLE
                     AGAEAPYQLVVTLLDSLRRNGVDSVGLSTRPAGQDAP"
     gene            complement(907..1578)
                     /locus_tag="PLW80_06800"
     CDS             complement(907..1578)
                     /locus_tag="PLW80_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPB66249.1"
                     /translation="MLDFIVKGGIILWIIMALSVVGLAIIIERVLYFRSIKTDEDKLF
                     QRVKASVEKGHFDEALSICDNNESPLSALIKVGIEYRAYPESAQKEVLKDAAAQEVPR
                     LERNVSTLGTIAHIAPLLGLLGTVTGTMKAFGVLGRFGSVADPSILAKGISEALITTV
                     GGIVVAVPAVIFYNLLVTKVNLILLKMENQVTALVMMINSGKRRGLASAAAAAKEDAA
                     RGAGA"
BASE COUNT          259 a          508 c          562 g          286 t
ORIGIN      
        1 aatagccaga tgggcaagta gaggctacgg ccgaccacgc ctgagccgga gaggaacgtc
       61 atgtcgtagg agttgccgct ctccgagccg cgtatcacca cgggctccgg cgccggcggc
      121 gcggctggct taggcgcggg cgctggctgg gtggtcggcg taggcttggc cgccgccggc
      181 ttcgcggtag gtagcgggct gggcgcggcg ggcgctggcg gttcgggcag ggccgcggcg
      241 ggcgcctcgg gtacgctctc ggcctgcgcg ctggaccgct cggcctcgac cggatgcggc
      301 gcgtcgacgc cgtcgggcga gccgagccgt acgatgatcg gcccggaaaa gtcggccata
      361 tcctgcacgg cgaacagcga caggagcacg gcgacgccga acaataggag atacgccccg
      421 agcgcagagg ataccgccac ggccgcgcgc ttccgctcgc gctcgcgcag ccactcgtcc
      481 cgggcgtctg tcatggggcg tcctgcccgg cgggacgcgt cgagaggccg accgagtcta
      541 cgccgttccg cctgagagag tcgagcagcg tgacgaccag ctggtacggc gcctcggcgc
      601 cggcctcgag cacggcctgc acttcgtcga cctgcttgcc ctccagctcc agcttgatca
      661 ccgactcggc gccctccgcg gtggtcctcg tcttgttgac gtagacctca tcctcggaca
      721 cggcgacgac cgacagctcg gtgatggtga tcgcggtggc ggtgccggaa tcgggtagcc
      781 gcagctcgat gcccggtgcg gtcttgaagg tgctggagat catgaagaag atgaccagct
      841 ggaagatgat gtcgatcagc ggcgtcaggt ctatgtttat ctggggcttg agccttcgtt
      901 ctatcgtcat gcgccggctc cccgggccgc gtcttccttg gccgcggccg cagccgatgc
      961 cagcccgcgg cgcttgccgg agttgatcat catgacgagg gcggtcacct ggttctccat
     1021 cttgagcagg atcaggttga ccttggtgac gagcaggtta tagaagatca ccgctggcac
     1081 ggctaccacg atgccgccga ccgtggtgat cagcgcttca gagatgccct tggccaggat
     1141 ggacgggtcg gcgaccgacc cgaagcgccc gagcacgccg aaggccttca tggtgccggt
     1201 cacggtgccg agtaggccaa ggagcggggc tatgtgcgct atcgtaccga gcgtcgagac
     1261 gttgcgctcg agtctcggca cctcctgggc ggcggcgtcc ttgagtacct ctttctgggc
     1321 cgactcggga tacgcgcggt actctatgcc gaccttgatc agcgccgaga gcggggactc
     1381 gttgttgtcg cagatcgata gggcctcgtc gaaatggccc ttctctaccg acgccttgac
     1441 ccgttggaac agcttgtcct cgtcggtctt gatgctcctg aagtagagga cgcgctcgat
     1501 gatgatcgcg aggcctacga ccgacagcgc catgatgatc cagagtatta taccgccctt
     1561 gactatgaaa tcgagcatgc gagcctccga ggactaggga tggatgccgg gcgac
//
LOCUS       DAOOLN010000487         1611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_99107_length_1611_cov_2.468509, whole genome shotgun sequence.
ACCESSION   DAOOLN010000487 DAOOLN010000000
VERSION     DAOOLN010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99107_length_1611_cov_2.468509"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PLW80_06805"
     CDS             complement(<1..620)
                     /locus_tag="PLW80_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPB66250.1"
                     /translation="MAYKNFILVCGGTGCESSRADDIYKELIASAEALGVKDDVQIVK
                     TGCFGFCEKGPIVKVLPSESFYVEVKPEDAREIIAEQVVKGREVARLLYKDDRQKDRI
                     KVEDIQFYQKQFRIVLRNCGVINPENIDEYIARDGYVALEKVLSSMKPDDVIEQLKIS
                     GLRGSGGAGFPTWRKWMFSKGVQADQKYVVCNADEGDPGAYMDRST"
     gene            complement(637..1032)
                     /locus_tag="PLW80_06810"
     CDS             complement(637..1032)
                     /locus_tag="PLW80_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPB66251.1"
                     /translation="MAKMTLEQLRKLREEKKGDMQKRDVEGKSIQIIVGMGTCGIAAG
                     AKQSFDEIVKAIDENKLSGDVIIRQTGCMGLCHVEPTVEVVMPGMPATIYGRVDAATA
                     RDIVVKHVVGKTLVENHIYDRPAVDIVKK"
     gene            complement(1050..>1611)
                     /locus_tag="PLW80_06815"
     CDS             complement(1050..>1611)
                     /locus_tag="PLW80_06815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB66252.1"
                     /translation="AGGVDQVVLETGADLLALRELDQKLWVALACPVQGTELDEASLK
                     LIDTDGKGMDERELERAKDPFYTDGTKHARRKVGLGLPFLIQATEQAGGDFRISSRKG
                     SGTRVSFGFPVDGVDTPPLGDLPGLFLSAMCFDGDYELVIRRRAPARGVDYEIRRSEI
                     LEAAGPLDDASALVAVREFLASHEAS"
BASE COUNT          251 a          536 c          494 g          330 t
ORIGIN      
        1 atcgtcgagc ggtccatgta ggcgcccggg tcgccttcgt cggcgttgca gaccacgtac
       61 ttctggtcgg cctggacgcc cttggagaac atccacttgc gccaggtcgg gaagccggcg
      121 ccgccgctcc cgcggaggcc ggaaatctta agctgctcga tcacgtcgtc cggcttcatc
      181 gacgacagaa ccttctcgag agcgacgtag ccgtcgcggg cgatatactc gtcgatgttc
      241 tccggattga tcacgccgca gttgcgcagg acgatgcgga actgcttctg gtagaactgg
      301 atgtcctcga ccttgatgcg gtccttctgc ctgtcgtcct tatagagcag gcgcgcgacc
      361 tcgcggccct tgaccacctg ctcggcgatg atctcgcgcg cgtcctcggg cttgacctcg
      421 acgtagaacg actcggaggg caggaccttg acgatcgggc ccttctcgca gaaaccgaag
      481 cagccggtct tgacgatctg gacgtcgtcc ttgacgccca gcgcctcggc gctggcgatg
      541 agttccttgt agatgtcgtc ggcgcggctg gattcgcatc cggtgccgcc gcagaccagt
      601 atgaagttct tgtacgccat gatttcggcc tccgccctat ttcttgacga tatcgaccgc
      661 gggccggtcg tagatgtggt tctcgacgag cgtcttgccg acgacgtgct tgacgacgat
      721 atcgcgggcg gtggccgcgt cgaccctgcc gtagatcgtg gccggcatgc cgggcatcac
      781 gacctcgacc gtcggctcga cgtggcagag gcccatgcag ccggtctgcc tgatgatcac
      841 gtcgccggac agcttgttct cgtcgatcgc cttgacgatc tcgtcgaagc tctgcttcgc
      901 gccggcggcg ataccgcagg tgcccatgcc gacgatgatc tgtatgctct ttccttcgac
      961 gtcgcgtttc tgcatatcgc ccttcttctc ctcgcggagc ttccggagct gttcaagggt
     1021 catctttgcc atatgaatgc ttcctttcgt cacgaggcct cgtgagaggc caggaattct
     1081 cggaccgcta ccaacgcgct cgcgtcgtcc aggggccctg cggcctcgag tatctcggac
     1141 cgcctgatct cgtagtcgac gccgcgggcc ggggcccgtc gccgtatcac caactcgtag
     1201 tcgccgtcga agcacatcgc cgataggaaa aggccgggta gatccccgag cggcggcgta
     1261 tcgacgccgt ctaccgggaa gccgaacgag acgcgcgtac ccgaaccctt gcgggaggag
     1321 atccggaagt cgcccccggc ctgctccgtc gcctggatga ggaacggtag ccctaggccg
     1381 accttcctcc tggcgtgctt ggtcccgtcg gtatagaacg ggtccttggc gcgctcgagc
     1441 tcgcgttcgt ccatgccctt gccgtccgta tcgatcagct tgaggctcgc ctcgtcgagc
     1501 tcggtgccct ggaccgggca cgcgagcgcg acccagagct tctgatccaa ctctcggaga
     1561 gccaagagat ccgcccccgt ctcgaggacc acttgatcca cgccgccggc a
//
LOCUS       DAOOLN010000488         1607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_99454_length_1607_cov_3.296392, whole genome shotgun sequence.
ACCESSION   DAOOLN010000488 DAOOLN010000000
VERSION     DAOOLN010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99454_length_1607_cov_3.296392"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..998
                     /locus_tag="PLW80_06820"
     CDS             117..998
                     /locus_tag="PLW80_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66253.1"
                     /translation="MFVPVTLAILAAILFYALVPIAGAFVVRQQWRLFRKSVAEASTL
                     PELDSRSAARSTLGRVRALGEVDAISGLDELWIRCEGAACAVDLKDAWVYLLVGRSGD
                     DTVERRRWSDLRAITPGSRAFVAGEAEMRDGRVVMAQRDKSPPLVILHDGDDDRVVRR
                     AIWAGRHENEYWNPVTQVSLAFGAAAMSAIVPLALPGRMPSLISALTLTAAFSPLLPL
                     LPPGVVGFFAYRRFWRRARYCRARRDLERLDDPTGAETEVWRRRAGTATLASALAFAG
                     SLAVNGWLIVAALRRLL"
     gene            complement(982..>1607)
                     /locus_tag="PLW80_06825"
     CDS             complement(982..>1607)
                     /locus_tag="PLW80_06825"
                     /inference="COORDINATES: protein motif:HMM:NF015817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HPB66254.1"
                     /translation="ASVARQPAKQPAKQAQAKKEEKPVREPETREPRDLRENRYRGEG
                     VTLFVSAGRRQRFYARVAVKVLLDIPGVADEQIGDIRTMDNYSFIVVDPAVEEAVIAT
                     LSGYDFKGRVLAVNRARKRGESAPSREPRTIDAVGEEPAEAPSDDSLYRDADEPDDSA
                     YGEDGDELDEDGLEPFGAAPEDDAEAPSDEYGDEPEGDPDEDPTEGA"
BASE COUNT          206 a          563 c          552 g          286 t
ORIGIN      
        1 gcctggattc tcgacgatat agacgttttc ccctatgggg cctacactca tgctgcggat
       61 catgcccggc gatactaccc ggccgggcgc ctcgatgaca agcggagcgc tacccgatgt
      121 tcgtaccggt gaccctggcc atactggccg ccatactctt ctacgcgctc gtgcccatag
      181 ccggcgcctt cgtcgtacgc cagcagtggc gcctctttag gaaatcggtc gccgaggcct
      241 cgacgctgcc cgaactggac tcgcgatcgg ccgcccgatc gacgctcggc cgcgtccgcg
      301 cgctgggcga ggtcgacgcc atcagcggcc tggacgaact ctggatacgc tgcgagggcg
      361 ccgcctgcgc ggtcgacctg aaggacgcct gggtctacct actcgtcggc agatcggggg
      421 acgacactgt cgagcgccga cgatggagcg accttcgggc catcacgccg ggttccaggg
      481 ccttcgtcgc cggcgaagcc gagatgcggg acggccgcgt cgtcatggcc caacgggaca
      541 agtcgccgcc gctggtcatc ctgcacgatg gggacgacga ccgggtcgtc aggcgggcca
      601 tctgggcggg ccgtcacgag aacgagtact ggaacccggt cacccaggta tcgctagcct
      661 tcggggcggc ggcgatgagc gccatagtac cgctcgcgtt acccggcagg atgccttcgc
      721 tgatcagcgc gctcaccctg acggcggctt tctcgccctt gctgccgctc ctgccgcccg
      781 gcgtggtcgg gttcttcgcg taccggcgct tctggagacg ggcacgctat tgccgtgcca
      841 ggcgcgacct ggaacggctc gacgacccta ccggcgccga gaccgaggtc tggcgccgac
      901 gggcgggcac ggccacgctg gccagcgcgc tcgctttcgc gggttcgctg gccgtcaacg
      961 gatggttgat agtagcggcg ctcaggcgcc ttctgtaggg tcctcgtcgg gatcgccctc
     1021 gggctcgtcg ccgtactcgt ccgacggcgc ctccgcatcg tcctccggcg ccgcgccgaa
     1081 cggctcgagg ccgtcttcgt ccagctcgtc gccatcttcg ccgtacgcgg agtcgtccgg
     1141 ttcgtcggcg tcccgataga gcgagtcgtc ggacggcgcc tcggcgggct cctcgccgac
     1201 cgcgtcgatc gtccgcggct cccgggaggg ggcgctctcg ccgcgcttcc tggcgcggtt
     1261 gaccgcgagc acgcggccct tgaagtcgta cccgctcagg gtggcgatga cggcttcctc
     1321 gacggccggg tcgacgacga tgaagctgta gttgtccatc gtcctgatat cgccgatctg
     1381 ctcgtcggcc acgccgggga tatcgagcag gaccttgacg gcgaccctgg cgtagaagcg
     1441 ctgcctgcgg ccggcgctca cgaacagcgt gacgccctcg ccgcggtagc ggttctcgcg
     1501 caggtcccga ggttcccggg tttcaggctc gcggacgggt ttctcttcct tcttggcctg
     1561 ggcctgcttg gcgggctgct tggcgggctg cctggcgacg ctcgcgg
//
LOCUS       DAOOLN010000489         1607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_99506_length_1607_cov_2.131443, whole genome shotgun sequence.
ACCESSION   DAOOLN010000489 DAOOLN010000000
VERSION     DAOOLN010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99506_length_1607_cov_2.131443"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="PLW80_06830"
     CDS             <1..1066
                     /locus_tag="PLW80_06830"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HPB66255.1"
                     /translation="DVAFRRAMNFLEARRLDDGLVLRRVSCPIGLIALIFESRPDALV
                     QMAALAVKSGDAIIVKGGSEAERTNRALAAIVAAAGVDAGLPSGWLVQLETRSEVARL
                     LALDGLVDLVIPRGSNEFVRHIMETSRIPVLGHAGGVCHVYVHGDADIAMAAAVVVDS
                     KAQYPAACNAVEVLLVDEAAARSALPELVGALERADVALELCPRSMAVVGPGPTRALK
                     DDDDWSVEYLALRMAVRVVGSLDEAIAHINRYGSGHTDAIVCSSGEAAARFMAEVDSA
                     SVYRNASTRFADGYRYGLGAEVGISTGKLHARGPMGLEGLQTYKWLLEGEGHIVSEYS
                     SGARRFIHEDLAVGKGGIRG"
     gene            1059..>1607
                     /locus_tag="PLW80_06835"
     CDS             1059..>1607
                     /locus_tag="PLW80_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66256.1"
                     /translation="MAKANSFLDARLAHEARAALASAKRIVVKIGTATITRQKGQAGR
                     ISDGPDPGGSGIDVEFLHRVAAELADLARDGRQPVLVTSGAVGMGARALGLEKKPKDT
                     ASKQACAAIGQPLLMEEYRRAFDVFGLTAAQLLVTREAWEDREAYLNLRSTVEALLER
                     RAVPVFNENDSVSTAEIAFGDND"
BASE COUNT          265 a          520 c          568 g          254 t
ORIGIN      
        1 cgacgtcgcg ttccggcgcg cgatgaactt cctcgaggcg aggcggctcg acgacggcct
       61 cgtcctcaga cgggtctcct gcccgatcgg cctcatcgcc ctgatcttcg agagccgccc
      121 ggacgccctc gttcaaatgg cggcgttggc ggtcaagagc ggcgacgcga tcatcgtcaa
      181 gggcggtagc gaggccgagc gcacgaaccg cgccctcgcg gctatcgtcg cggccgccgg
      241 cgtagacgcg ggcctgccct cgggctggct agtccagctg gagacacgaa gcgaggtcgc
      301 ccggctcctg gccttggacg gtctcgtcga cctggtcata ccccgcggct ccaacgagtt
      361 cgtcaggcat atcatggaga cgagtaggat cccggtgctc ggccacgcag gcggcgtctg
      421 ccacgtctat gtccacggcg acgctgatat agccatggcc gctgcggtgg tagtcgatag
      481 caaggcccaa tacccggcgg cctgtaacgc cgtcgaggtc ctgctcgtcg acgaggcagc
      541 ggcgcggtct gcgctaccgg agcttgtcgg agccctggaa agggctgacg tcgcgctcga
      601 gctgtgcccg aggagcatgg ccgtcgtcgg cccgggtccg accagggcgc tcaaggacga
      661 tgacgactgg tcggtcgagt acctggcgct ccgcatggct gttcgcgtcg tcggctcgct
      721 cgatgaagcg atagcccata tcaaccgcta cggctcgggc cataccgacg ccatcgtctg
      781 ttcgtcaggc gaggcggcgg cgcgcttcat ggccgaggtc gactcggcgt cggtctaccg
      841 caacgccagc acccgcttcg ccgacggcta ccgctacggg ctcggggccg aggtcgggat
      901 ctccaccggt aagctacacg cacgggggcc gatggggctc gagggcctgc agacctataa
      961 gtggctactc gagggcgagg ggcatatagt ctcggagtac tcgtccggcg ccaggcgctt
     1021 tatccacgag gacctggcgg tagggaaagg cgggatccgt ggctaaggct aactcgttcc
     1081 tcgatgcgag gctagcccac gaggcgaggg cggccctggc ctcggccaaa cgcatcgtcg
     1141 tcaagatcgg caccgcgacg ataaccaggc agaaggggca ggcgggcagg atatcagatg
     1201 gccccgatcc gggaggctcc ggtatcgacg tcgaattcct gcaccgcgtc gccgcggagc
     1261 tggccgacct ggcgcgcgat ggcaggcagc ccgtgctcgt gacctctggc gcggtaggca
     1321 tgggcgcgag ggcgctcggc ctcgagaaga agcctaagga caccgcctcg aagcaggcct
     1381 gcgcggcgat cgggcagccg cttctgatgg aggagtaccg gagggcgttc gacgtattcg
     1441 gcctgaccgc ggcgcagctc ctggtgaccc gcgaggcttg ggaggatcga gaagcctacc
     1501 tgaacctgcg ctcgacagtc gaggcgctgc tcgagcgcag ggcggtgccg gtgttcaacg
     1561 agaacgactc ggtttccacc gccgagatcg cctttggcga taacgac
//
LOCUS       DAOOLN010000490         1607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_99511_length_1607_cov_1.951675, whole genome shotgun sequence.
ACCESSION   DAOOLN010000490 DAOOLN010000000
VERSION     DAOOLN010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99511_length_1607_cov_1.951675"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..109)
                     /locus_tag="PLW80_06840"
     CDS             complement(<1..109)
                     /locus_tag="PLW80_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin FeS4 iron-binding
                     domain-containing protein"
                     /protein_id="HPB66257.1"
                     /translation="MTTGNKVYRCAKCGNVVELLHEGGGELVCCGEPMKL"
     gene            complement(121..696)
                     /locus_tag="PLW80_06845"
     CDS             complement(121..696)
                     /locus_tag="PLW80_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPB66258.1"
                     /translation="MKSVKGTQTEKNLMEAFAGESQARNRYTYFASKARDEGYMQIAA
                     VFEETANQEKEHAKRLFKLMDGGVISINGTFPAGPVGTTSENLLEAAKGEDHEWQEMY
                     PGFAKTARAEGFDAIATIFEAIAVAERQHSKRYSGLKANVDAGVAFKRTAPVVWQCRN
                     CGYIVEAVSAPQACPACAHPQSYFELLGENW"
     gene            868..1461
                     /locus_tag="PLW80_06850"
     CDS             868..1461
                     /locus_tag="PLW80_06850"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HPB66259.1"
                     /translation="MKHAIESYCWAGEASGQKPDIRERLIASGTPSVADYELVAAIIG
                     AGSRGKDVRTLSTEVLAGCDFSAGVPSVSSISSIPGMGEARACRVAAAFELGKRFYGT
                     RERRIASPNDAWQLVRHFDDRKQERFLCCTLNGAHDVIAVRVITVGLVNRTIVHPREI
                     YAEAVADRACAILIGETYFYTMTTMTLIPLLSFLLIK"
BASE COUNT          325 a          507 c          429 g          346 t
ORIGIN      
        1 ggagcttcat cggctcgcca cagcagacga gctcgccgcc gccctcgtgg agcagttcca
       61 cgacgttacc gcacttggcg caacggtaca ccttgtttcc cgtggtcatg atgtccctcc
      121 ttaccaattc tcgccgagta gctcgaaata cgactgggga tgagcgcagg ccgggcaggc
      181 ctggggtgcc gatacggcct cgactatgta gccacaattc ctgcactgcc agacgacggg
      241 ggccgtacgc ttgaaggcga cgcccgcgtc gacgttcgcc ttgagccccg aatagcgctt
      301 ggaatgctgc cgctcggcca cggcgatcgc ctcgaagatg gtagcgatgg cgtcgaagcc
      361 ctcggctcgg gccgtcttgg cgaagccggg atacatctcc tgccactcat ggtcctcgcc
      421 cttcgccgcc tcaaggaggt tctccgaggt cgttccgacc gggccagcgg ggaaggtgcc
      481 gttgatgctt atgactcccc catccatcag cttgaacagc cgcttcgcgt gctccttctc
      541 ctggttcgcg gtctcctcga acacggcggc tatctgcata tagccctcgt cgcgcgcctt
      601 gctcgcgaag tacgtatagc gattcctcgc ctgcgactcg ccggcgaaag cctccatcag
      661 gttcttttct gtctgggttc ccttaacgct tttcatcttg cccccgatcc tcatgaattc
      721 ctcccggcca acgccgggac gcatacacca gcgattcgaa agtatacaat caaatatcgc
      781 tggaagcaaa tagagcatac agttttagtc atgtcctaga tcgaaagcgc cgcggctccg
      841 ccgagcgtac cggccgcgga ggcatcgatg aaacacgcga tcgagtcata ctgttgggcg
      901 ggcgaggcgt cgggccaaaa accggatatc agggagaggc tcatagcatc gggaactccg
      961 tccgtcgccg actacgaact ggtcgccgcc atcatcggcg ccggttcgag aggcaaggac
     1021 gtgcgaacgc tatcgacgga ggtgctcgcc ggatgcgact tcagcgccgg cgtcccgagc
     1081 gtctcgagca tatcgtcgat cccgggcatg ggcgaagcgc gagcctgccg cgtagccgcg
     1141 gctttcgagc tcggtaagcg cttctatgga acgagggaac gcaggatcgc gtccccgaac
     1201 gacgcctggc agctcgtccg ccacttcgac gaccgaaaac aggaacgctt cctctgctgc
     1261 acgctgaatg gcgctcacga tgtaatcgcc gtccgcgtca taaccgtcgg cctcgtcaac
     1321 aggaccattg tccatcctcg cgagatctac gcggaagccg tggccgaccg cgcctgcgcc
     1381 atcttgatag gtgagacata tttttacaca atgaccacta tgacactaat accattactg
     1441 tcatttctat taataaaatg agcacctttt ctaaattgtg tttaaatcaa tcgatgagcg
     1501 ccattcatcc aaatcctagt aactagtaac atatcctaac cgaccctcct gccttcctct
     1561 ctaaaggcaa cttctaatat atcttttttt tattggttac tggttac
//
LOCUS       DAOOLN010000491         1606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_99555_length_1606_cov_3.078014, whole genome shotgun sequence.
ACCESSION   DAOOLN010000491 DAOOLN010000000
VERSION     DAOOLN010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99555_length_1606_cov_3.078014"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PLW80_06855"
     CDS             <1..826
                     /locus_tag="PLW80_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB66260.1"
                     /translation="MALDPGQGVAGYNLSRALAEAGKYDESIAMSDRYLDSDPDNAAV
                     LAVKAYALHRQGRDDEAIAVYERVASLNGGDRASSFNLAVLLEATGRGDEAMARYDAM
                     LALDPDDAAASYRKGLLLAGGGDAEAAIPFLERYLAAEPESAEGRRVLAAALERAGLY
                     LRALEAYAAMAAKDETDAAAWFALARLRLTKADDATGGLSALDSAIEQGFKDAEAARA
                     LLRTDGLLAADLVEKRLGRVGLLDGERADGASADGVSADGASDGAPKDEGAGASGS"
     gene            823..>1606
                     /locus_tag="PLW80_06860"
     CDS             823..>1606
                     /locus_tag="PLW80_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66261.1"
                     /translation="MNRPAARLAPACAALALCAATAAGQATVVFRTAPPNVALLDASA
                     PGLDTALRLRAADNGGRSAHLTAGTHEITLIAEGYRPARVAVEVAEGPGAPAVVVEAK
                     LERAASVLRLARVIATGRRPKSVEFTPDGRYVVVAPLSGEAVEVIDPRTGTLVANLAP
                     PERWARSQGFVETAFPGRGEVWVSQMYTDLLHVFSLADYSYLRSVSSGGSFPKVLLAH
                     PDGRVFASNWLSETVSALSPDGMTRLGSMRVGSTPRGLALSPD"
BASE COUNT          205 a          578 c          598 g          225 t
ORIGIN      
        1 catggccctc gacccgggtc agggcgtggc cggctacaac ctgtcacggg cgctcgccga
       61 ggcgggcaag tacgacgagt cgatcgcgat gagcgaccgc tacctggact cggatcccga
      121 caacgcggcc gtgctcgcgg tcaaggccta cgcgctgcac cggcaaggcc gtgacgacga
      181 ggcgatcgcc gtctacgagc gcgtcgcttc gctgaacggc ggcgaccgcg cgagctcgtt
      241 caacctagcc gtgttgctcg aggccaccgg tcgcggggac gaggcgatgg cccgctacga
      301 cgcgatgctc gccctagacc cggatgacgc ggccgcctcg taccgcaagg ggcttctcct
      361 cgctggcggc ggcgacgccg aggcggccat cccgttcctg gagcgttacc tggccgccga
      421 gccggagtcg gccgaggggc gacgggtcct ggcggccgcg ctcgagcggg ccgggctgta
      481 cctgcgtgcc ctcgaggcct acgccgcgat ggcggccaag gatgaaaccg acgcggcggc
      541 ctggttcgcg ctggccaggc tcaggctgac caaggccgac gacgccacgg gcgggctgtc
      601 ggcgctcgac tcggctatcg agcagggctt caaggacgcc gaggccgccc gcgccctgct
      661 ccgtacggac gggctactcg cggccgatct cgtcgagaag cggctcgggc gcgtcgggct
      721 cctcgacggg gagcgggctg acggcgcatc ggctgacggc gtgtcggccg atggcgcgtc
      781 ggacggcgcg cccaaggacg agggcgccgg ggccagcggc tcttgaaccg cccggccgcg
      841 aggctcgcgc cggcctgcgc cgccctggcg ctctgcgcgg cgaccgccgc cggccaggcg
      901 acggtcgtgt tcaggacggc cccgcctaac gtggccctgc tcgacgcgtc ggcgccgggg
      961 ctggacacgg cgctacggct gcgggccgcg gataacggcg gccggtccgc gcacctaacg
     1021 gccgggacac acgagatcac gctgatagcc gagggctacc ggccggcccg cgtcgccgtc
     1081 gaggtcgccg aggggccggg cgcgccggcc gtcgtcgtcg aggccaagct cgagcgcgcc
     1141 gcctcggtgc tgcggctggc tcgcgtgata gcgacgggtc ggcggccgaa gagcgtcgag
     1201 ttcaccccgg acggccggta cgtcgtcgtc gcccctttgt ccggtgaagc tgtcgaggta
     1261 atcgatccgc gcaccgggac tctcgtcgcc aacctggcgc ctccggagcg ctgggcgagg
     1321 agccagggct tcgtcgaaac ggccttccct ggccgtggcg aggtctgggt cagccagatg
     1381 tacaccgacc tcctgcacgt attctcgttg gccgactact cgtacctccg ctcggtatcc
     1441 tcgggcggat ccttcccgaa ggtgcttctc gcccaccccg acgggcgggt cttcgcgtcg
     1501 aattggctgt ccgagaccgt cagcgcgctg tcgcccgatg gcatgacccg gctcggctcg
     1561 atgcgcgtgg gttcgacccc gcgcgggctg gccctgtcgc cggacg
//
LOCUS       DAOOLN010000492         1603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_99816_length_1603_cov_3.779070, whole genome shotgun sequence.
ACCESSION   DAOOLN010000492 DAOOLN010000000
VERSION     DAOOLN010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99816_length_1603_cov_3.779070"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..>1603
                     /gene="uvrC"
                     /locus_tag="PLW80_06865"
     CDS             134..>1603
                     /gene="uvrC"
                     /locus_tag="PLW80_06865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPB66262.1"
                     /translation="MDENKARPSAGLRNSAKQAPEAPGVYLWKDADGVVIYVGKAKSL
                     KARLASYFTGKREIKTRHLVAKASTLEWILTETEYEALLLENNLIKRYAPKYNINLKD
                     GKTYPSIRITNEEYPRVFRTRRIVDDGSRYFGPYPSADVIDRYLELVRRLFPLRRCKT
                     LRRRETPCMYYHIGRCSAPCAGKTTREEYALQVERVATLLSGDSEALVEALRADMAAA
                     SEALQFEKAAYLRDSVQAIESFRGDSGVVDFAYGDRDYLAWAVDGSLVDFVVFQTRSG
                     RLVGRDLYRERSFGSEAEAASEFMAAYYGPERLPPPEVFVKDAEGLELAADFLRRGLG
                     AAFRLAAPDERRHEAALAMAEHNAREDLAKRRRESGDVEALEALRAALGLEVLPELIE
                     GFDIAQLSGKHTVASLVSFRNGAANKKGYRIYKIKSLDGGIDDFGAMREATARHYTHV
                     ANDEAEAPDLVLIDGGRGQVNAAWEILDALGLDLPVVGLA"
BASE COUNT          280 a          541 c          525 g          257 t
ORIGIN      
        1 ccttgaatcc ggtgcctcga tgcttgaagg gtgcgctcat gctgttcgtt cctcgatatc
       61 gatggggaat cggccctatg gccggggagg ctccacattc aggcgaaacc ccgttatcat
      121 attatcgaag attatggacg agaataaagc ccggccttcc gccggattga ggaatagcgc
      181 caaacaagcc cccgaggcgc ccggtgtcta cctctggaag gacgccgacg gcgtcgtcat
      241 ctacgtcggc aaggccaagt cgctcaaggc gaggctcgcc tcgtacttca ccggcaagcg
      301 cgagatcaag acgaggcacc tcgtcgccaa ggcctcgact ctcgagtgga tactcacgga
      361 gaccgagtac gaggccctgt tgctcgagaa caacctgatc aagcgctacg cgcccaagta
      421 caacatcaac ctgaaggacg gcaagaccta tccgtcgata cgcatcacca acgaggagta
      481 cccgagggtc ttcaggacga ggcgcatcgt cgacgatggt agccgctact tcgggcccta
      541 cccgagcgcc gacgtcatcg accgctacct ggagttggtc aggcgcctgt tcccgctccg
      601 tcgctgcaag acgctcaggc ggcgcgagac gccgtgcatg tactaccata tcggccgttg
      661 ctccgccccc tgcgccggca agacgacccg cgaggagtac gccctgcagg tcgagcgggt
      721 agcgacgctc ctgtccgggg acagcgaggc cctggtcgag gcgctcaggg ccgacatggc
      781 cgccgcgtcc gaggcgctac agttcgagaa ggccgcctac ctgcgcgact cggtccaggc
      841 gatcgagtcg ttccggggcg actcgggcgt cgtcgacttc gcatacggcg accgcgacta
      901 cctggcttgg gcggtcgacg gtagcctcgt cgacttcgtc gtgttccaga ctcgctcggg
      961 gcgcctcgtc ggccgcgacc tgtaccgcga gcgtagcttc ggctccgagg ccgaggccgc
     1021 ttccgagttc atggcggctt actacggccc cgagcgcctg ccgccgcccg aggtcttcgt
     1081 caaggacgcc gagggcctgg agctggccgc cgatttcctt cgtcgcggcc tcggcgccgc
     1141 gttcaggctg gccgcgccgg acgagcgccg ccacgaggcc gccctcgcta tggccgagca
     1201 caacgcccgc gaggacctgg ccaagcggcg gcgcgagtcg ggcgacgtcg aggctctcga
     1261 ggccctgcga gccgccctcg gcctcgaggt gctgcccgag ctgatcgagg gcttcgacat
     1321 agcccagctg tcgggtaagc atacggtcgc ctcgctggtg tcgttcagga acggcgccgc
     1381 caacaagaaa ggctaccgta tctataagat caagagcctc gatggcggca tcgacgactt
     1441 cggggcgatg cgcgaggcga cggcccggca ctacacccac gtggccaacg acgaggccga
     1501 ggcgcccgac ctggtgctca tagacggcgg ccgcggccag gtgaacgcgg cttgggagat
     1561 actcgacgcg ctcggcctgg atctgccggt cgtcggcctg gcc
//
LOCUS       DAOOLN010000493         1603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_99841_length_1603_cov_2.608527, whole genome shotgun sequence.
ACCESSION   DAOOLN010000493 DAOOLN010000000
VERSION     DAOOLN010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99841_length_1603_cov_2.608527"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PLW80_06870"
     CDS             complement(<1..249)
                     /locus_tag="PLW80_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPB66263.1"
                     /translation="MTEKTFMAGFGGQGVISMGQLWVYCGMKEDLEVTFFPFYGAEKR
                     GGIARASVIVSTEEIASPLVTRPDSAIVMNMDSLPLCEA"
     gene            complement(246..1016)
                     /locus_tag="PLW80_06875"
     CDS             complement(246..1016)
                     /locus_tag="PLW80_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPB66264.1"
                     /translation="MELVYGHPKSLKPVQTKYCPGCGHGVIHRLITEVIDEMGMQEKS
                     IILNPVGCSIWASLYFDFDSVQVPHGRTPAGATAVKRMLPDHLVICYQGDGDLAAIGT
                     AEAVHAANRGEKFTTIFVNNAIYGMTGGQMAPTTLVGQKATTAPYGRDPVEAGMGYPI
                     RVLEMLATLGGTKYLARGAVNNAANVRKTKQYIKKAFEAQMRGEGSSMVEILSQCPTN
                     WAMDPVDSVGWVESAMMPFYALGEVKNELAGAGKEAGK"
     gene            complement(1027..>1603)
                     /locus_tag="PLW80_06880"
     CDS             complement(1027..>1603)
                     /locus_tag="PLW80_06880"
                     /inference="COORDINATES: protein motif:HMM:NF028456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HPB66265.1"
                     /translation="WRMPAMILADGMIGQMMEGVTLPEAKDPATRKAKPWAVGNKGKR
                     TELNHVDSTQLVPENFEKALHVRYARYDEIKKTVVRWEEQNLEDADLILSAYGTSARV
                     CLGAMQMARKLGLKVGLLRPITLWPFPEQRFGALAGKRFLVVEMSMGQMVEDVRLYTH
                     GKSEVSFYGRCGGMIPTEEDVLAEIRRILKK"
BASE COUNT          237 a          529 c          526 g          311 t
ORIGIN      
        1 cgcctcgcag aggggcaggg aatccatgtt catcacgatg gcgctgtcgg gcctcgtgac
       61 gagcgggctg gcgatctcct cggtggagac gatgacgctg gcgcgggcga tgccgccgcg
      121 cttctcggcg ccgtagaacg ggaagaaggt gacctcgagg tcctccttca tgccgcagta
      181 tacccacagc tgccccatcg atatcacgcc ctggccgccg aagccggcca tgaaagtctt
      241 ctcggtcatt taccggcctc cttgcccgcg cccgccagtt cgttcttgac ctcgccgagc
      301 gcgtagaacg gcatcatcgc gctctccacc cagccgacgc tgtcgacggg gtccatcgcc
      361 cagttggtag ggcactgcga caggatctcg accatgctcg agccctcgcc gcgcatctgg
      421 gcctcgaagg ccttcttgat gtactgcttg gtcttgcgca cgttggcggc gttgttcacg
      481 gcgccgcggg ccaggtactt ggtaccgccg agggtggcga gcatctcgag cacgcggatc
      541 gggtagccca tgccagcctc gaccgggtcg cggccgtagg gcgcggtggt ggccttctgg
      601 ccgacgaggg tggtcggggc catctgaccg ccggtcatgc cgtagatggc gttgttcacg
      661 aagatggtcg tgaacttctc gccgcggttg gcggcgtgca cggcctcggc ggtaccgatg
      721 gcggccaggt cgccgtcgcc ctggtaacag atgaccaggt ggtcgggcaa catgcgcttg
      781 acggcggtgg cgccggccgg cgtgcggccg tgcggcacct gcaccgagtc gaagtcgaag
      841 tacaggctcg cccagataga gcaaccgacc gggttcagga tgatcgactt ctcctgcatg
      901 cccatctcgt cgatgacctc ggttatcagg cggtgaatga cgccgtggcc gcagccgggg
      961 cagtacttgg tctggaccgg cttgaggctc ttgggatgtc cgtatacgag ttccacgctc
     1021 gcccccttac ttcttcagga tccggcggat ttcggcgagt acgtcctctt ccgtcgggat
     1081 catgccgccg cagcggccgt agaacgaaac ttcgctctta ccgtgcgtat acaggcgcac
     1141 gtcctccacc atctgcccca tgctcatctc gacgaccagg aagcgcttgc cggccagcgc
     1201 gccgaagcgc tgctccggga acggccacag cgttatcggc cggaggaggc cgaccttgag
     1261 tccgagcttc ctggccatct gcatcgcgcc caggcagacg cgggccgagg taccgtaggc
     1321 gctcaggatg aggtccgcgt cctcgaggtt ctgctcttcc cagcgcacga cggtcttctt
     1381 gatctcgtcg tagcgcgcgt agcgcacgtg cagggccttc tcgaaattct cggggaccag
     1441 ctgggtcgag tcgacgtggt tgagctccgt tcgtttgccc ttgttgccga cggcccaggg
     1501 cttagccttg cgcgtcgccg ggtccttggc ctcgggcagc gtgacgccct ccatcatctg
     1561 gcctatcatg ccgtcggcca ggatcatcgc gggcatgcgc cac
//
LOCUS       DAOOLN010000494         1601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_100027_length_1601_cov_2.876455, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000494 DAOOLN010000000
VERSION     DAOOLN010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100027_length_1601_cov_2.876455"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PLW80_06885"
     CDS             complement(<1..519)
                     /locus_tag="PLW80_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HPB66266.1"
                     /translation="MSIATSQQIAKYYETFKTISVTYTKEIVKSTGLQPQNVFLKCLG
                     EQWPCVIYSSSFSEVKVLAANRPALLERIAKANNLVSVRLSFKQSDTSEPVQFFISGR
                     VAGFSPYAQSNGTLQFVSIQLTHRPPDDFVEIMGRLLEANVNSARRRDDRILLTPDVM
                     RKIGLARKESTIL"
     gene            679..>1601
                     /locus_tag="PLW80_06890"
     CDS             679..>1601
                     /locus_tag="PLW80_06890"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HPB66267.1"
                     /translation="MKEEYEFPLEDYMSRERFERMKRFSEDKATPCLILDLDVVREKY
                     EELRRNMPYAKVYYAVKANPNDEVVALLRDLGSCFDVATRFELDQLLRLGVTPDRMSF
                     GNTIKKERDIAYFYEKGVRLYATDSFEDVDKIARAAPESKVFFRILTEGLGADWPLSR
                     KFGSHPEMTRNLILHARDLGLEPYGISFHVGSQQRDIGQWSTALGNVGNIYSWAEDQG
                     VKLRMINMGGGFPAMYTDPTNPIEHYAGDIRSFLTNNFGDELPEIIIEPGRSMTGDCG
                     MIVTEIVNIAKKSTHDLYHWVFFDIGKFGGL"
BASE COUNT          340 a          468 c          488 g          305 t
ORIGIN      
        1 caggatcgtc gactccttgc gggccaggcc tatcttgcgc atcacgtcgg gcgtcaggag
       61 tatgcgatcg tcgcggcggc gcgcggaatt gacgttggcc tcgagcagtc ggcccatgat
      121 ctccacgaaa tcgtccggcg ggcggtgcgt cagctgtatc gagacgaact gcagcgtacc
      181 gttcgactga gcgtacggcg agaagccggc cactcgaccc gatatgaaaa actggaccgg
      241 ctcggaggtg tccgactgct tgaacgacag ccggacactg accaaattgt tggccttggc
      301 gatgcgctcc agcaaggccg gcctgttggc cgccagcacc ttgacttcgc tgaacgagct
      361 cgagtagatg acgcacggcc actgctcccc gaggcacttc aggaacacgt tctgaggctg
      421 gagcccggtg gacttcacga tttccttggt ataggtcacc gagatggtct tgaaggtctc
      481 gtagtatttg gcgatctgct ggctggtggc tatggacatg atcagctcga ataatacggt
      541 atggcctatc tttggtcaat caaaggggat ggatggaccg gctataaacg atattgacaa
      601 agggggtggc tgaacctatc ttttgcgcat gcggcgcctg gggcgcggcc atcaaaccgc
      661 tacgttaagg taggagcgat gaaagaagag tacgagttcc cgctcgagga ttatatgagc
      721 agggagcgct tcgagcggat gaagcgattc tccgaggata aggcgacccc ctgccttatc
      781 ctggacctag acgtcgtacg ggagaagtac gaggagctcc gccggaacat gccgtacgcc
      841 aaggtctatt acgcggtcaa ggctaacccg aacgacgagg tagtcgcctt gctgcgggat
      901 ctcggctcct gcttcgacgt ggccacgcgc ttcgagctgg atcagctgct caggctcggc
      961 gtgacgccgg accgcatgag cttcgggaac acgatcaaga aagagcgcga catcgcctat
     1021 ttctacgaaa agggcgtcag gctctacgcc accgatagct tcgaggacgt ggacaagata
     1081 gcccgcgccg cgccggagtc caaggtgttc ttcaggatcc tcaccgaggg cctcggagcc
     1141 gattggcccc tgtcgaggaa attcggttcc caccccgaga tgacgcgcaa cctgatactc
     1201 catgcccgcg acctcgggct ggagccctac ggcatctcgt tccacgtcgg gagccagcag
     1261 cgcgacatcg gccagtggtc gacggcgctc ggcaacgtag gcaacatcta ctcctgggcc
     1321 gaggatcagg gcgtcaagct gcgcatgatc aacatgggcg gcggcttccc ggccatgtat
     1381 accgatccga ccaaccccat cgagcattac gcgggcgaca tcaggtcctt cctgaccaac
     1441 aacttcggcg acgagctacc cgagatcatc atcgaacccg gtcggtcgat gaccggcgac
     1501 tgcggtatga tcgtcaccga gatcgtcaac atcgccaaga agagcactca cgacctgtac
     1561 cattgggtct tcttcgacat aggtaaattc ggcgggctca t
//
LOCUS       DAOOLN010000495         1600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_100111_length_1600_cov_3.007767, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000495 DAOOLN010000000
VERSION     DAOOLN010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100111_length_1600_cov_3.007767"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="PLW80_06895"
     CDS             <1..1119
                     /locus_tag="PLW80_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPB66268.1"
                     /translation="RGVELEARRAGIRLPVRPHAVFVHGYEVPRWGGPLVRWLLPRVG
                     AMPVHHSKLDSVGMARIRAAIEDGPFPLALSPEGQVSYTADDVPRLEPGAVRIGLQAA
                     DRLAKAGRAARVELMPISVRYRYGPRARRSLLRLLARLESFVGVGAGSDDEPVVDRLR
                     RCLEMILAEGERQYGLASADAAGVAEGAERGVAARVGAVVEAALDAGERLLGIERGPG
                     DSIERLYRIRQIGWDRVFLPPEEDPRNVSGMRRAVLDRRAGEAWYAMRHMELADFAWY
                     LRSGPPAADGGLTTGPIVEYAQNLWDFANRLAGGAISGRREVRPKRAVVTASAPIDLS
                     ARLEDYRRDRKSAQAAIMDELKRSYTACIKEFINEERG"
     gene            1103..>1600
                     /locus_tag="PLW80_06900"
     CDS             1103..>1600
                     /locus_tag="PLW80_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB66269.1"
                     /translation="MKSAVETAGRLPVRTKVGFGIADLGGNLFFTAMGFWSMFYLTDG
                     VGIPAAMAGLAVMIGKIWDAVTDPMMGYISDRTDSKLGRRRPYIIFGALPLMATMVYF
                     FKVPAFTNPVALTVWATAALCLLNTAYTVVNVPYASLTPELTKDYNERTSLNGYRFAF
                     AAVGTM"
BASE COUNT          254 a          513 c          566 g          267 t
ORIGIN      
        1 cgcggcgtcg agctcgaggc ccgacgagcc ggcatcaggc tacccgtccg gccccacgcg
       61 gtgttcgtgc atggctacga ggtgcccagg tggggcggcc ctctcgtccg ttggctgcta
      121 cctcgggtcg gggccatgcc ggtacaccat agcaagctcg actcggtcgg aatggccagg
      181 atccgcgccg ccatcgagga tggtcccttc cctctcgcgc tatcgccgga gggccaagtg
      241 agctatacgg ccgacgacgt gccgaggctc gagccgggcg ccgtacgtat cggtctccag
      301 gcggccgacc ggctcgccaa ggccgggcgc gcggcccgcg tggagctgat gccgatatcg
      361 gtacgatacc gttacgggcc gcgcgcccgg cgctcgctac tacggctcct ggcccggctg
      421 gaatccttcg tcggcgtcgg ggccggaagc gacgacgagc cagtagtcga ccggctacga
      481 cgttgcctcg agatgatcct ggccgagggc gagcgtcagt acggcctcgc gtcggccgac
      541 gccgctggcg tcgcggaagg cgccgagcgc ggagtggcgg ccagggtcgg cgccgtggtc
      601 gaggcggccc tggatgccgg ggaacggctt ctcggtatag agcgggggcc tggcgactcg
      661 atcgagcggc tgtaccgtat caggcagata ggctgggatc gcgtgttcct gccgccggaa
      721 gaggatcctc ggaacgtttc gggcatgcgg agagctgtac tggaccggag agccggagag
      781 gcttggtacg cgatgcggca tatggagctg gccgacttcg cctggtacct gcgctcgggg
      841 cctcccgccg ccgacggcgg cctgacgaca gggcctatcg tcgagtacgc ccagaacctc
      901 tgggacttcg cgaatcggct agccggcggc gcgatatccg ggcggcgcga ggtgcggccg
      961 aagcgggccg tcgtgaccgc gtccgcgccg atagacctga gcgcgcggct cgaagattat
     1021 aggcgcgacc ggaagagcgc ccaggcggcc atcatggacg agcttaaacg atcgtatacg
     1081 gcatgcataa aggagttcat aaatgaagag cgcggttgaa acggccgggc gcttgcccgt
     1141 ccgcacgaaa gtaggcttcg gcattgccga cctgggcggt aacctgttct ttaccgcgat
     1201 ggggttctgg tccatgttct acttgaccga cggcgtcggg atcccggccg cgatggccgg
     1261 actggccgtg atgatcggca agatctggga cgcggtgacc gacccgatga tgggttatat
     1321 ctccgaccgg acggattcca agttgggccg gcgccggccg tacatcatct tcggcgcctt
     1381 gccgctgatg gcgacgatgg tctatttctt caaggtaccg gccttcacga acccggtcgc
     1441 gctgaccgtc tgggcgaccg cggcgctctg cctcctcaat accgcctaca cggtcgtcaa
     1501 cgtgccctac gcgtcgctga cgccggagct gaccaaggac tataacgagc ggaccagtct
     1561 gaacggctac cgcttcgcct tcgccgccgt cggcacgatg
//
LOCUS       DAOOLN010000496         1598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_100290_length_1598_cov_3.061568, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000496 DAOOLN010000000
VERSION     DAOOLN010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100290_length_1598_cov_3.061568"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PLW80_06905"
     CDS             complement(<1..475)
                     /locus_tag="PLW80_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66270.1"
                     /translation="MTNDSNVLDYNRTAVREAIVGALKKRAGEAAPADVVAFTGLPKP
                     QVDAELPAVADEYSGRLKVTESGEILYSFPDGFHSRYRGFGPTARRIARVAGKAAVKV
                     GTFLFKAWIMVMLVGYFALFIALVLLSLVASVAANASDRDGKSRSRGGGLALTGRL"
     gene            complement(512..>1598)
                     /locus_tag="PLW80_06910"
     CDS             complement(512..>1598)
                     /locus_tag="PLW80_06910"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPB66271.1"
                     /translation="RLCDALRWKPDVFHAHDWPGGFLPAYLRYAEPGYASSASLLSIH
                     NLGYQGIYPKDAYPYFGLPWELFHGAGFEYYDSINALKAGIVCADGLSTVSPTYAREI
                     QTPEFGCTLDGLLRSRGADLVGILNGVDAQDWDPAHDRYLPVRYSTDDLRGKAACKAA
                     LQREFGLPEDPDKPLFGMVSRLTDQKGIGELFGPTYGCVEGMCRDMDLQFVVLGSGEP
                     WCERAIVELAGRLPNFGSRIGYSEPAAHLIEAGSDFFMMPSRYEPCGLNQMYSLRYGT
                     LPVAHRTGGLADTISNFDQDSGAGTGFLFDCLTPRAVYDTAGWAVWAFYNRPGQIEAM
                     RKRAMAIDFSWAASAREYESLYAGLVS"
BASE COUNT          288 a          516 c          541 g          253 t
ORIGIN      
        1 cgagtcggcc ggtcagggcc aagccgccgc cgcggctccg gctcttgccg tcccggtccg
       61 acgcgttagc ggccacggag gccacgagcg acagcagcac gagggcgatg aacaaggcga
      121 agtaaccgac gagcatgacc atgatccaag ccttgaacaa gaaggtcccg accttgacgg
      181 ccgccttgcc agcgaccctc gcgatacgcc tggcggtcgg cccgaaaccc ctataacggc
      241 tatggaaacc gtccgggaag gagtacagaa tctctccgga ctcggtcacc ttgagccggc
      301 ccgagtactc gtcggcgacg gccggtagct cggcgtccac ctgcggcttc ggtagcccgg
      361 tgaaggctac gacgtccgcc ggcgcagcct cgcccgcgcg cttcttgaga gcgccgacta
      421 tggcctccct gaccgcggtc ctattgtaat cgagcacgtt cgaatcgttc gtcatcgcgt
      481 ctcccctatc gctattccga caatgctaac atcaactgac gaggcccgcg tagagcgact
      541 cgtactctcg ggccgaggcc gcccacgaga aatcgatggc catcgcgcgc ttcctcatgg
      601 cctcgatctg cccgggccgg ttgtagaagg cccagacggc ccagccggcc gtgtcgtaga
      661 cggcgcgcgg ggtcaggcag tcgaacagga agccggtgcc ggcgccgctg tcctggtcga
      721 agttcgagat ggtgtccgcg aggccaccgg tccggtgggc tacgggcaag gtgccgtagc
      781 gtagcgagta catctggttg agtccgcacg gctcgtagcg gctcggcatc atgaaaaagt
      841 cagagcccgc ctcgatcagg tgggccgcgg gctcggagta gcctatcctc gagccgaagt
      901 tcggaagtcg acccgccagc tcgacgatgg cgcgctcgca ccagggctcg cccgagccca
      961 ggacgacgaa ctgcaggtcc atgtcccggc acatcccctc gacgcagccg taggtggggc
     1021 cgaagagctc gccgatgccc ttctggtcgg tcaagcgcga caccatgccg aagaggggct
     1081 tgtccggatc ctcgggtagg ccgaattcac gctgcagggc cgccttgcag gccgccttgc
     1141 cgcggaggtc gtcggtcgag tagcggacag gcaggtagcg gtcgtgggcc ggatcccaat
     1201 cctgggcgtc caccccgttg agtatgccga cgaggtcagc gccccgggag cgcaggaggc
     1261 cgtccagggt gcagccgaat tcgggcgtct ggatctcgcg cgcgtaggta ggcgaaacag
     1321 tcgacaaccc gtcggcgcag acgatgcccg ccttgagcgc gttgatcgag tcgtagtact
     1381 cgaagccggc gccgtggaac agctcccatg gcaggccaaa gtatggatag gcgtccttgg
     1441 ggtagatacc ctggtaaccg aggttatgga tgctcaggag gctggccgat gaggcgtagc
     1501 caggctccgc gtagcgcaga taggccggca ggaaaccgcc tggccaatcg tgggcgtgga
     1561 agacgtcggg cttccagcgt agcgcgtcgc atagcctg
//
LOCUS       DAOOLN010000497         1595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_100568_length_1595_cov_3.055844, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000497 DAOOLN010000000
VERSION     DAOOLN010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100568_length_1595_cov_3.055844"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..862
                     /locus_tag="PLW80_06915"
     CDS             212..862
                     /locus_tag="PLW80_06915"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPB66272.1"
                     /translation="MVLVDDDGGLGREPPVRGYCVIPAAGRSSRMGDWKPLLPWGRST
                     ICGAVADAALAAGLRPVVVAGYRADELAAAFSGRPEVAVVVNRDWERGMLGSVRVGAA
                     LPLSRGSGFFVAPADMPRLPARAFERALAELDRLEQAGEATRTLFASRAGRLGHPVWI
                     PASVAVGLGALDPGLRLRDHLLGLPWSAVEVDDDGIFEDLDTPEAYREAIARILTE"
     gene            901..>1595
                     /locus_tag="PLW80_06920"
     CDS             901..>1595
                     /locus_tag="PLW80_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66273.1"
                     /translation="MKTKHTMLAIAVMALASAAGAQTPSRVVMGGKAVTMVADAVYMF
                     PSARPRVVAIGGTDQGLGVFLETVAPGFSALPSFPRQAGVEVYASHRPDLVILKSSLK
                     KSLGAGLEALGIRTLYLSLETPDDYYAELALLGRTFGDEKRAAELIGYYQNVVATAAG
                     EGVVAGPGVPRVGPALEHQHPGNRVAADDGGHGGLGDRKRLHGFCRVVHRGDRAGRGG
                     SGPLVRACGNLGP"
BASE COUNT          230 a          514 c          571 g          280 t
ORIGIN      
        1 ccgagtattt gagccgctcg gcgatcaggt acgattctat ggtcgacagc ttcgtggcca
       61 cgcgttcttc tcctcgggta aacggtttca aaggctacta cgtatagtat atcgagaagc
      121 cggcccgata tgaaaggtcg agcgcggttc taatcgaatc atccgatccg gaggccttgc
      181 ggcgtccgtc acgctgccgc atactcgcgt catggtttta gttgacgatg acggcggtct
      241 gggccgcgag ccgccggtca ggggctattg cgtcataccg gccgcagggc gctcgtcgag
      301 gatgggcgac tggaagcccc tgctgccctg gggccggtcg acgatatgcg gcgcagtggc
      361 cgacgcggcc ctggcagccg gcctgcggcc cgtcgtcgtg gccggctacc gggcggatga
      421 gctggccgcc gcgttctcgg gccggcccga ggtcgcggtc gtcgtcaacc gggattggga
      481 gcgcggcatg ctcggctccg tgcgggtcgg cgcggccctg cccctgagcc ggggctcagg
      541 cttcttcgtg gcgcccgcgg atatgccgag gctgccggcc cgggctttcg aacgggcgct
      601 cgccgagctg gaccggctcg agcaggcagg cgaggccact cggaccctgt tcgcctcgag
      661 agccgggcgc ctcggccacc cggtctggat acccgcttcg gtcgcggtag gactcggcgc
      721 cctcgacccg ggactgcgcc tgcgcgatca cctgctcggg ctaccgtggt ctgccgtcga
      781 ggtggacgac gacggcatct tcgaggacct ggatacgccc gaggcgtatc gagaggctat
      841 agccagaata ctcactgaat gagcttgtgt cgattcgctt tggcgtgcta ggattgcggc
      901 atgaaaacga agcatacgat gctcgccatc gccgtcatgg cgctggcgtc ggctgcggga
      961 gcccagacgc cctcgcgggt ggtcatggga ggcaaggccg tcacgatggt cgctgacgcc
     1021 gtttatatgt tcccctccgc gcgcccgcgt gtcgtagcga tcggcggcac cgaccagggc
     1081 ctcggggtgt tcctggagac ggttgcgccg ggtttctccg cgctaccgtc gttcccgcgc
     1141 caggccggcg tcgaggtata cgcgtcccat aggccggacc tcgtcatact caagtcgagt
     1201 ctcaagaaaa gtctcggcgc cgggctcgaa gcgctcggga tcaggaccct gtacctgtcc
     1261 ctcgagacgc ccgacgacta ctacgccgag ctggccctgc tcggtaggac cttcggcgac
     1321 gagaagcggg ccgcggagct catcggctac taccagaacg tcgtcgcgac ggccgccggg
     1381 gaaggagtag tggccggacc aggggtcccg cgggttggcc cggcgcttga gcaccagcac
     1441 cccgggaacc gggtcgcggc tgacgatggt ggccacggcg gcctcggtga tcggaaacga
     1501 ctgcatgggt tctgccgggt tgtgcaccgc ggcgaccgtg ccgggcgcgg gggatccggt
     1561 ccgctggtcc gcgcatgcgg gaacctgggg ccgtt
//
LOCUS       DAOOLN010000498         1594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_100648_length_1594_cov_3.126706, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000498 DAOOLN010000000
VERSION     DAOOLN010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100648_length_1594_cov_3.126706"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..1387
                     /locus_tag="PLW80_06925"
     CDS             299..1387
                     /locus_tag="PLW80_06925"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPB66274.1"
                     /translation="MKPLPESESATSVKALAPVSRQRARYLALVGLSSALLVARVALT
                     SLSFDAVAVARAAVSGLALVVVGILAVDASLNKKRPLVARLSSKSRRLAAAAYLTTYA
                     VCVLDVAAGVDPYLFIPGMVVYCFFVWDRPGFLAVSAGLCAYAVTRAMAAFGRGVGAD
                     DFMMLAFGVIVALYAADTVKKSVLPSEERLRALSLENEELWNLSFRDTLTGLYNRRYI
                     QQIAIHLFSRAVRYKEQLHVLMIDIDHFKRVNDKLGHAVGDEVLKGVAQTIQATIRTS
                     DTVARFGGEEFIVYMVRSDPETTQFIANRIRDGVAAVQFPGVPWQITISVGVAGLQEN
                     DTIDSLVERSDQFLYVSKHRGRNRVSGF"
     gene            complement(1398..>1594)
                     /locus_tag="PLW80_06930"
     CDS             complement(1398..>1594)
                     /locus_tag="PLW80_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPB66275.1"
                     /translation="AHRMNLSVVAEGVEAAEQFYLLKSMGCDFAQGFLFSHALPPLEL
                     EGRYLSRGTGSRYSSFLLRR"
BASE COUNT          267 a          512 c          509 g          306 t
ORIGIN      
        1 gctgcgggcc ggctgcgact tctcgctgat cgggctcaag tcgtcgagcc tgtcactcga
       61 ggcaggctac gacctggccg gcggctcgtt cgccgctcgg ctgtacctgt cgccggtcgg
      121 ggggtactga gcccggcgac gttgcgaagg ttgacaagcg ggggcaaggc gcttacggtt
      181 aaacggtact cgtatgcgga gggtttcgat ggctcctcgg tgcaacggcc gcctcgctca
      241 cgtctcgccg tcgaagaccg ccgttatcat gacacgaata gaaaaaggct gatcatttat
      301 gaaaccattg cctgaatcgg aaagcgcgac gtcggtcaag gctctcgccc ccgtgtctcg
      361 tcagcgagcc cgctacctcg ctctcgtagg cctgtcgagc gctctcctcg tcgcgcgggt
      421 cgcgttgacc tcgctgtcct tcgatgccgt cgccgtcgcg cgggccgccg tatccggatt
      481 ggccctggtc gtcgtcggga tcttggcggt cgacgcgtcg ctcaacaaga agcggccgct
      541 cgtcgcccgc ctatcgtcca agagccgtcg cctcgcggcc gccgcctacc tgaccaccta
      601 cgccgtctgc gtcctcgacg tagccgccgg cgtcgatccg tacctgttca taccggggat
      661 ggtcgtctac tgcttcttcg tctgggaccg gcccggcttc ctggccgtat ccgcggggct
      721 atgcgcctat gccgtgacgc gggccatggc ggcctttggg cgaggcgtcg gtgccgacga
      781 cttcatgatg ctcgcgttcg gcgttatcgt ggccctgtac gccgccgaca ccgtgaagaa
      841 gagcgtgttg ccgagcgagg agcgactgcg cgccctgagt ctcgagaacg aggagctatg
      901 gaacctttcg ttcagggata cgctgaccgg cctctataat cgtcgctata tccagcagat
      961 cgccatccac ctgttttcgc gggccgtacg ctacaaggag cagctacacg tgcttatgat
     1021 agacattgac cattttaagc gggtcaacga caagctcggg cacgccgtcg gtgacgaggt
     1081 gctcaagggc gtggctcaga ccatccaggc caccatacgc acgagcgaca cggtagcccg
     1141 cttcggcggc gaggaattca tcgtgtacat ggtcaggtcg gatcctgaga ctacgcagtt
     1201 catagccaac aggatccgcg acggcgtagc cgcggtccag ttccccggtg tgccctggca
     1261 gatcaccatc agcgtaggcg tggccggcct ccaggagaac gacaccatag actcgctggt
     1321 ggaacggtcg gatcagttcc tctacgtgtc gaagcatcgc ggccgtaacc gcgtctccgg
     1381 cttttagccc gcggcgctta gcggcgtagc aggaacgacg agtagcgcga gccggttccg
     1441 cgggacaggt acctgccctc gagctcgagc ggcggtagcg cgtggctgaa caggaagccc
     1501 tgggcgaagt cgcagcccat ggacttgagt aggtagaact gctccgccgc ctccacacct
     1561 tcggcgacca ccgaaaggtt catgcggtgg gcca
//
LOCUS       DAOOLN010000499         1591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_100934_length_1591_cov_3.465495, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000499 DAOOLN010000000
VERSION     DAOOLN010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100934_length_1591_cov_3.465495"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..578)
                     /locus_tag="PLW80_06935"
     CDS             complement(93..578)
                     /locus_tag="PLW80_06935"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPB66276.1"
                     /translation="MIVEFILNGEDVSLKADPLERLSDLLRERFDLTSLMSDCREGVC
                     GKCVVFLDGLLVNSCMVPAFKARGTEVVTYEGFKSTESFATVERSFREAGAELCGFCD
                     SALFMAAGSLIEATVRPSEEEIMEVLSSVYCRCVTPSRALGAVKAALESSLGGKYGRA
                     R"
     gene            complement(568..>1591)
                     /locus_tag="PLW80_06940"
     CDS             complement(568..>1591)
                     /locus_tag="PLW80_06940"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPB66277.1"
                     /translation="GMDPYEWRAMNVARRGDQTLGPPLRKGAPLDAVCDRLLAISDYP
                     RKRSSFELIRKRRPDPSSPPGYGIGLAFGPQRPATALSCRDAQAPSVEVTLSKDSTLT
                     IRSSVASGSGAVTRIWKRIAATSLGLSPEAVEVATPSTDEVPDSGPNVFSRRIGVVTR
                     LIGSACDGLASKRFREALPITVRKSQRARSAKAAPESPLDDASWGAAVVELELDPVDA
                     APTVRGIWLVAKAGRILSREDAAAALERDAAVALGLCVAECLDLSAGPVDPGDLRRYG
                     LWRIKDAPPIQVEFVDDDAEPKGIGELAYSLVPAAFANALSQALDSPWNALPTCRRSE
                     GVDDDS"
BASE COUNT          252 a          546 c          551 g          242 t
ORIGIN      
        1 gtatcagcgt gccgcccgcc cagggcagcg cgccgggttg acgcttcaat acggcgacgg
       61 cctccgctat cgtctggggc gcggatacat cgtcaacgcg cacggccgta cttccctccg
      121 aggctcgact cgagcgccgc cttgaccgcc ccgagggctc gcgacggcgt gacgcaacgg
      181 cagtataccg aggacagtac ctccatgatc tcctcctcgg aggggcggac ggtcgcttcg
      241 atcaacgagc cggcggccat gaacagcgcc gagtcgcaga agccgcatag ctcggccccc
      301 gcctcgcgaa aggagcgttc gacggtcgcg aacgattcgg tgctcttgaa gccctcgtag
      361 gtgacgacct cggtgcccct cgccttgaag gcgggtacca tacaggaatt gaccaggagc
      421 ccgtcgagga agacgacgca cttgccgcag acgccctcgc ggcaatcgct cataaggctc
      481 gtcaggtcga agcgctcgcg taggagatcg gatagccgct cgagggggtc ggccttcagg
      541 ctgacgtcct ctccattcag gatgaattca actatcatcg tcgacgccct ccgatcggcg
      601 acaggtcggc aacgcgttcc acggcgagtc gagcgcctgg ctcagcgcgt tggcgaaggc
      661 ggcgggtacg agcgagtagg ccagctcgcc tatgcccttg ggctcggcgt cgtcgtcgac
      721 gaactccacc tgaatgggag gtgcgtcctt gatccgccag aggccgtatc gcctgaggtc
      781 gccaggatca accggcccgg cggacaggtc gaggcactcg gcgacgcaaa gcccgagcgc
      841 cacggcggcg tcgcgctcga gcgccgctgc ggcgtcctcg cgcgacagga tgcggcccgc
      901 cttcgccacg agccagatgc cgcgtaccgt cggggcggcg tccaccgggt cgagctcaag
      961 ctctacgacg gcggcgcccc atgacgcgtc gtcgaggggc gactcgggcg cggccttggc
     1021 ggacctggcc cgctgcgact tcctgacggt gatcggcagc gcttccctga agcgcttgga
     1081 ggccagaccg tcgcaggcgg agcctatcag cctcgtcacg acgccgatcc gccgcgagaa
     1141 cacgttcggc cccgagtcgg gtacctcgtc ggtgctgggc gtcgcgacct ccaccgcctc
     1201 gggcgagagc ccgagggacg tcgcggctat ccgcttccag atcctcgtca cggcgcccga
     1261 gcccgacgcc acgctcgagc ggatggtcag cgtcgagtcc ttggacaggg tcacttcgac
     1321 cgagggagcc tgggcgtcgc ggcaggaaag cgccgtggcc ggccgctgag ggccgaaggc
     1381 cagcccgatg ccgtagccgg gcggggacga cgggtcgggg cgtcgtttgc gtatcagctc
     1441 gaacgacgat cgcttgcgcg ggtagtcgct gatggcgagc agcctgtcgc agaccgcatc
     1501 gagcggcgcg cccttcctga gcggtgggcc gagcgtctgg tcgccgcgcc tggccacgtt
     1561 catggcgcgc cattcgtatg gatccatacc g
//
LOCUS       DAOOLN010000500         1589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_101186_length_1589_cov_2.267927, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000500 DAOOLN010000000
VERSION     DAOOLN010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101186_length_1589_cov_2.267927"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PLW80_06945"
     CDS             <1..589
                     /locus_tag="PLW80_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPB66278.1"
                     /translation="NLLAMNAAIEAARAGEAGKGFAVVADEIRKLSASTSEQSKSIKG
                     MLAEVAGTIGDIAESSREAGSNFDGIRDLIRTVTRLEEEIRQAMEEQSAGSNRILSSL
                     DSMKQASRAMDGEAEAVADIAGQISLETEALTRYSTNIQESISGVGTCAASIRQAVAK
                     AEELTTMNKASIDRMDERLGVFRLKGEADEADEPA"
     gene            complement(586..>1589)
                     /locus_tag="PLW80_06950"
     CDS             complement(586..>1589)
                     /locus_tag="PLW80_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HPB66279.1"
                     /translation="SRAAVRGSIATGLRALALCALGLSAVAAGAQPLAGGAGAGGVAF
                     REVWAYLMRGEERFLGAGLPITDLCLFSAEINAYGELVGVPPAASVSSFPGRKHLVVA
                     EIGSYSLTHFCLDPAFPIRAALVDAIVAAAEPFDGVQIDFEAIPVRDRDNFVTFLGQL
                     KAGLGPRILSVAVPARLSAAGDALGYARLAAVADRIVVMAYDEHWSTSEPGPVASMDW
                     CRSVAAYARSVVPVDRLVMGLPFYGRAWGDRRTNRALKYSAVEELIAEKGIGVFFRES
                     GIPRVRYQETVNVDVYYDDAESLLERMRLYRDSGVRAVAFWRLGQEDPAVWKGVGRD"
BASE COUNT          287 a          546 c          553 g          203 t
ORIGIN      
        1 gaacctcctg gcgatgaacg ccgccatcga ggcggcccgc gccggcgagg cgggcaaggg
       61 cttcgcggtc gtggcggacg agatacgcaa gctgtcggcg agcacgagcg agcagtcgaa
      121 gagcatcaag ggaatgctcg ccgaggtggc cggcacgata ggcgatattg ccgagtcgtc
      181 ccgcgaggcg gggtcgaact tcgacggcat acgcgacctg atacgcacgg tcacgaggct
      241 cgaggaggag atcaggcagg cgatggagga gcagagcgcc ggctccaacc gcatcctatc
      301 gtccctcgac tcgatgaaac aggcgtccag ggcgatggat ggcgaggccg aggccgtcgc
      361 cgacatagcg ggccagatat cgctcgagac cgaggccctg acccgctact cgacgaatat
      421 ccaggagagc atctcgggcg tcgggacctg cgcggccagc atccgccagg cggtggccaa
      481 ggccgaggag ctgacgacga tgaacaaggc gagcatcgac cgcatggacg agcggctcgg
      541 ggtgttcagg ctcaagggcg aagcggacga agcggacgag ccggcctagt ctcggcccac
      601 gcccttccat acggccgggt cctcctggcc gaggcgccag aacgctaccg cccggacgcc
      661 cgagtcgcgg tagagccgca tccgctcgag cagggattcg gcgtcgtcgt agtacacgtc
      721 gacgttgacc gtctcctggt accggacgcg cgggatgccg ctctcgcgga agaacacgcc
      781 gatgcccttt tccgcaatca gctcctcgac cgccgagtac ttgagcgccc ggttcgtacg
      841 ccggtctccc caggcccggc cgtagaacgg cagccccatc acgagccgat cgaccggcac
      901 gacggaacgg gcataggcgg ccacggaccg gcaccagtcc atggaagcca ccggccccgg
      961 ctcgctcgtg ctccagtgct cgtcgtaggc catcacgacg atacggtcgg cgacggccgc
     1021 tagcctggcg tagccgagcg cgtctccggc tgcggacagc ctggccggga ccgcgacgct
     1081 caggatcctg gggccgaggc ccgccttgag ctgcccgagg aaggtcacga agttatcgcg
     1141 gtctctgacc ggtatcgcct cgaagtctat ctgcacgccg tcgaagggct cggccgccgc
     1201 gacgatggcg tcgacgaggg ccgcccgtat cgggaaggcc gggtcgaggc agaagtgcgt
     1261 cagcgagtag ctgcctatct cggccaccac gaggtgtttc cgcccaggga acgacgatac
     1321 cgacgcggcc ggcggcaccc cgacgagctc gccgtacgcg ttgatctccg cgctgaatag
     1381 gcacaggtcg gtaataggca ggccggcgcc gaggaaacgc tcctcgccgc gcatcagata
     1441 ggcccagacc tcgcggaagg ctaccccgcc ggcccccgct cctccggcca ggggctgggc
     1501 gcccgccgcg accgccgaca ggccgagagc gcagagggcc agcgcgcgga gccccgtggc
     1561 gatcgatccc ctcacggccg ccctactcg
//
LOCUS       DAOOLN010000501         1588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_101234_length_1588_cov_3.470320, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000501 DAOOLN010000000
VERSION     DAOOLN010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101234_length_1588_cov_3.470320"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PLW80_06955"
     CDS             complement(<1..394)
                     /locus_tag="PLW80_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB66280.1"
                     /translation="MPDRRRLVVIVTGASSGLGNACATQLAKRGYAVYGTSRDPASRE
                     RKADEFFELIRLESSDDDSVSTAVGYVLAKEGRLDALVCCAGMGIAGPIEETPVVEAA
                     TQMDVNFMGVARVVRAALPSLRESGGRLV"
     gene            complement(387..944)
                     /locus_tag="PLW80_06960"
     CDS             complement(387..944)
                     /locus_tag="PLW80_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB66281.1"
                     /translation="MSDFTESARKPSIIAISGKSGCGNSTVSRLVTERLGRQLVNYTF
                     HTMADEMGVPFAELLEMAKADPSYDRRLDERQVEMAHRGDCVIGSRLAIWLVRDAELK
                     VYLLASSDVRARRIQQREGGEYADVLAFTKRRDESDRERYLSIYGIDNDRYDFADLVI
                     NTERLSAERIADIVIAAIGRGPDCA"
     gene            complement(941..>1588)
                     /locus_tag="PLW80_06965"
     CDS             complement(941..>1588)
                     /locus_tag="PLW80_06965"
                     /inference="COORDINATES: protein motif:HMM:NF015699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HPB66282.1"
                     /translation="PTDVVIDASSAKAVADAIRRLGQAAGIDEPVRLSHLLGVDGMLS
                     YERNVDADLLWTALEPALNACLDEFDAEREREGAATRADLEEKLATLGWALDVIEAAG
                     PGIEAGLKAALRAKFEEVMGDLVDESRVLGELASYLAKHTINEEIVRLRSHMAAFRQA
                     MGEPVCGKKLDFICQEMNREANTIGSKSADARVSDAVIRMKDAVENIREQARNVE"
BASE COUNT          230 a          549 c          527 g          282 t
ORIGIN      
        1 cgactaggcg gccgccgctc tcccgtaggc tgggcagggc ggccctgacc acgcgagcga
       61 cgcccatgaa gttcacgtcc atctgggtag ccgcctcgac gacgggcgtc tcctcgatcg
      121 ggcctgcgat gcccatgccg gcgcagcata cgagggcgtc cagacgtccc tccttggcca
      181 gtacgtagcc cacggcggtc gatacggagt cgtcgtccga cgactcgagc cgtatcagct
      241 caaagaactc gtccgccttg cgctcgcgcg aggcgggatc ccggctcgta ccgtagacgg
      301 cgtagccgcg cttggccagt tgggtggcgc aggcgttgcc gaggccgctc gacgcgccgg
      361 tgacgatgac gaccagccgg cgacgatcag gcacagtccg gccctcggcc tatggccgcg
      421 atgacgatgt ccgctatccg ctcggcgctc aggcgctcgg tgttgatgac caggtcggcg
      481 aagtcgtagc ggtcgttgtc gatgccgtag atcgacaggt agcgctcgcg gtccgactcg
      541 tcgcgacgct tcgtgaacgc gagtacgtcg gcgtactcgc ctccctcgcg ctgctggatg
      601 cgcctggcgc gcacgtcgct cgaggcgagg aggtagacct tgagttcggc gtcgcggacc
      661 agccagatgg cgagcctcga gccgatcacg cagtcgccgc ggtgggccat ctcgacctgc
      721 cgctcgtcga gcctgcgatc gtacgacgga tcggccttgg ccatctccag gagctcggcg
      781 aacggcacgc ccatctcgtc cgccatcgta tggaaggtat agttgacgag ctggcgtccg
      841 agccgctcgg tgaccagcct cgagacggtc gagttgccgc agccgctctt acccgatatg
      901 gcaatgatcg acggcttcct ggcgctttcg gtgaaatcgc tcattcgacg ttcctcgcct
      961 gctcgcggat gttctcgacc gcgtccttca tgcggatgac cgcgtccgaa accctcgcgt
     1021 cggcgctctt ggagccgatg gtgttggcct cgcgattcat ctcctggcag atgaagtcga
     1081 gcttcttgcc gcagaccggc tcgcccatcg cctggcggaa ggcggccata tgcgagcgca
     1141 gcctgactat ctcctcgttg atcgtatgct tggccaggta ggaggccagc tcgccgagca
     1201 cgcgcgactc gtcgaccaag tcgcccatga cctcctcgaa cttggcccgc aaggccgcct
     1261 tcaggccggc ctcgatgccg gggcccgccg cctcgatcac gtcgagggcc cagccgaggg
     1321 tcgccagctt ctcctcgagg tcggcccggg tggccgctcc ctcgcgctcg cgctcggcgt
     1381 cgaactcgtc gaggcaggcg ttcagggccg gttccagggc ggtccagagc aggtccgcgt
     1441 cgacattgcg ctcgtagctc agcatgccat cgacgccgag caggtgcgaa agcctgaccg
     1501 gctcgtcgat cccggccgcc tggccgagcc tgcgtatggc gtccgctacc gccttggcgc
     1561 tcgaggcgtc gatgaccacg tcggtggg
//
LOCUS       DAOOLN010000502         1588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_101292_length_1588_cov_1.904762, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000502 DAOOLN010000000
VERSION     DAOOLN010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101292_length_1588_cov_1.904762"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLW80_06970"
     CDS             <1..409
                     /locus_tag="PLW80_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HPB66283.1"
                     /translation="ALRRVAAGHADGEAVGVLSTTGTRRSGVYDELLSRAGYEAIYVP
                     EGEQETLHAAIYDPSWGIKAVSPVSDRAARIVADMASRLIGDGASAVLLACTELPLAL
                     PGTDLGGAPLVDPVLALARALVREAAPEKLRPL"
     gene            complement(372..719)
                     /locus_tag="PLW80_06975"
     CDS             complement(372..719)
                     /locus_tag="PLW80_06975"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPB66284.1"
                     /translation="MDDIALRRYELRTEIFKALAHPMRIYMLDKLRERPWCVCELAAS
                     VGVDKSVASKHLSQLKAAGLVDDERRGTLVEYRLVAPCVLDMSECAERTILGNKRARL
                     EAIAAVASPAPLP"
     gene            823..1074
                     /locus_tag="PLW80_06980"
     CDS             823..1074
                     /locus_tag="PLW80_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPB66285.1"
                     /translation="MKVQILGSGCPKCKLLEQHAREAVAELGVDAEIVKVTNTDDIME
                     MGVMMTPALAIDGEVKSVGKVLTKDQVASYLKVVKFHAL"
     gene            1100..1432
                     /locus_tag="PLW80_06985"
     CDS             1100..1432
                     /locus_tag="PLW80_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HPB66286.1"
                     /translation="MIYACSGAANTGLLADQVMRILSRNKVGSSTCLAAMGADLSGFL
                     ESARNATRNIVLDGCKVSCGKRIFEKNGLAYQHYVMTDYDVEKGKTPITGDLTEAVAE
                     RIEGLVKA"
BASE COUNT          306 a          471 c          513 g          298 t
ORIGIN      
        1 agccctgcgg cgagtcgctg ctggccatgc ggacggtgag gccgtcggcg tgctgtcgac
       61 gaccggtacc aggcgttcgg gcgtctacga cgagctgctg tcccgggcgg gctacgaggc
      121 gatctacgtg ccagagggcg agcaggagac gctccacgcc gccatctacg acccgtcctg
      181 gggcattaaa gccgtctcgc cggtcagcga ccgagcggcc cggatcgtgg ccgacatggc
      241 ctcccggctg ataggcgacg gcgcgtccgc cgtcctcctg gcctgtactg aactaccgct
      301 agcgctaccg ggtaccgatc tgggtggcgc gccgctcgtc gacccggtgc tggcgctcgc
      361 ccgcgccctg gtcagggaag cggcgccgga gaagctacgg ccgctatagc ctccagccgc
      421 gcgcgcttgt tgccgagtat cgtgcgctcg gcgcattcgg acatatcgag tacgcacggc
      481 gccaccagcc ggtattccac gagggtgccg cgtcgttcgt cgtcgacgag gccggcggcc
      541 ttgagttgcg acaggtgttt ggacgccact gacttgtcga caccgacgct ggcggccagc
      601 tcgcagacgc accaggggcg ctcccgcagc ttatcgagca tgtatatcct catcgggtgg
      661 gccagagcct taaaaatttc cgtccgcagt tcatagcgtc gcaatgctat atcgtccata
      721 gactgaatgt tgcaatgttt ctaaacaatt gtcaagaagg tacttgacaa ttgtgtgcat
      781 tgtttcaata atttgcaaca tgaggttgca atggaggatt ctatgaaggt acagattctt
      841 ggttccggtt gtcccaaatg caagctactg gaacagcacg cccgcgaggc ggtagccgag
      901 ctcggcgtag acgcggagat cgtcaaggtg acgaataccg acgacatcat ggagatgggc
      961 gtgatgatga cgcccgcgct ggcgatagac ggcgaggtca agagcgtcgg caaggtgctg
     1021 acgaaggacc aagtcgcctc gtacctgaaa gtcgttaagt ttcatgccct gtaagtgcga
     1081 cggtacttgc gaaaccactt tgatctacgc gtgctcggga gcggcgaata ccgggttgct
     1141 cgccgaccag gtgatgcgga tcctgagtcg gaacaaggtc ggctcgagca cctgcctggc
     1201 cgcgatgggc gccgacctct cgggcttcct tgagtcggcg cgcaacgcga cgcgcaatat
     1261 cgtgctggac ggctgcaagg tatcctgcgg caagaggata ttcgagaaga acggcttagc
     1321 gtaccagcac tacgtcatga ccgactacga cgtcgagaag ggaaagacgc ccataaccgg
     1381 cgacctgacc gaggccgtgg ccgagcgtat agaggggctg gtcaaggcat gagctccaag
     1441 gaaatctccc ccaaccagaa actgctcatc gtcttcggcg tcttcctcgc ggcgtacttc
     1501 ataccgtggg gcgcggcgcg cgtcctcggc gcgacgaacg aggcgttcct gatgctcggc
     1561 gagtatgcta ggcagcacgt gctcctgt
//
LOCUS       DAOOLN010000503         1586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_101409_length_1586_cov_3.370346, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000503 DAOOLN010000000
VERSION     DAOOLN010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101409_length_1586_cov_3.370346"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /gene="ftsA"
                     /locus_tag="PLW80_06990"
     CDS             <1..832
                     /gene="ftsA"
                     /locus_tag="PLW80_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPB66287.1"
                     /translation="LDMIGVRLEAEVHIITGSVTTAQNLVRCVNRAGFKADGLILQSL
                     AASRAVLTADEKELGCLLVDLGGGTTDVLMYSEGAPYFSASVGAGGSQVTNDLSIILN
                     VPMDAAERIKRESGCCYLPHVELDESIVVPGVGGRPPQEIAKAKVASVIQPRMEEIFR
                     LAREKVEQPGFWRHVKGGVVLTGGGALMPGAAELAYEVFGVPVRIGAPASLGGLVDAY
                     RNPAYSTAVGLVLLGSERLGAPEPDARGKASGAEKAKAQKSRPDAPFKRLGEWLKEFF
                     "
     gene            910..>1586
                     /gene="ftsZ"
                     /locus_tag="PLW80_06995"
     CDS             910..>1586
                     /gene="ftsZ"
                     /locus_tag="PLW80_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPB66288.1"
                     /translation="MTLRVVDEVGAFRRAEAADTTNAPRGDAGQEQCNATVIKVIGAG
                     GGGSNAVNRMIEAGLRNVEFIVANTDLQALAKSRADVKLGLGAKVTHGLGAGGKPEVG
                     EKAAIEDRAMIAEAVQGADMVFITAGMGGGTGTGSAPIIAEVAKEQGALTVAVVTKPF
                     DFEGKVKMRLAEEGIERLRQAVDTLIVIPNQHLLKVVEKRTPIREAFKLADDVLRQGV
                     QGISDLIT"
BASE COUNT          277 a          493 c          592 g          224 t
ORIGIN      
        1 gttggacatg ataggcgtca ggctcgaggc cgaggtgcac atcatcaccg gttcggtgac
       61 gacggcccag aacctggtcc gttgcgtcaa ccgcgccggc ttcaaggccg atgggctgat
      121 actccagagc ctcgcggcgt cgcgggccgt gcttacggcc gacgagaagg agctgggctg
      181 cctgctcgtc gacctcggcg gcggcaccac ggacgtactt atgtactcgg agggcgcccc
      241 gtacttcagc gcgtcggtgg gagccggcgg ctcgcaggtc acgaacgacc tgtcgatcat
      301 cctgaatgtg ccgatggacg ccgccgagcg catcaagcgc gagtcaggct gttgctacct
      361 gccgcacgtc gagctcgacg agagcatcgt cgtgccggga gtcggcggca ggccgcccca
      421 ggagatagcg aaggccaagg ttgccagcgt gatccagccg cgcatggagg agatattcag
      481 gctggctcgc gagaaggtcg agcaacccgg cttctggcga cacgtcaagg gcggcgtcgt
      541 gctgaccggc ggcggcgcgc tgatgcccgg ggcggcggag ctggcgtacg aggtgttcgg
      601 ggtgccggtc aggatcggcg cgccggcgtc gctcggcggg ctcgtcgacg cgtaccgcaa
      661 cccggcgtac tcgaccgcgg tcggcctcgt gctgctgggt tccgagcgtc tcggcgcgcc
      721 cgagccggac gcgaggggca aggcctccgg cgccgagaag gccaaggctc agaagtcccg
      781 gccggacgcg ccgttcaagc gcctcggcga atggctcaag gagttcttct gaattaggat
      841 acggccggct ccggctcgct gacacgggcg ggggtcggat atttgtaaac gagcatacgg
      901 gaggggaata tgacgctacg ggtcgtggac gaggtcgggg cgttcaggcg ggcagaggcc
      961 gccgatacga cgaacgcgcc gcgcggggac gccgggcagg aacagtgcaa cgccacggtc
     1021 atcaaggtga tcggcgccgg cggcggcggg tcgaacgcgg tcaacaggat gatcgaggcg
     1081 gggctgcgca acgtcgagtt catcgtcgcc aatacggacc tgcaggcgct cgccaagtcg
     1141 cgagccgacg tgaagctggg gctcggcgcc aaggtgaccc acggactcgg cgccggcggc
     1201 aagcccgagg tcggcgagaa agccgcgatc gaggaccggg cgatgatcgc cgaggccgtc
     1261 cagggagccg acatggtctt catcaccgcc ggtatgggcg gcggcaccgg taccggctcc
     1321 gctccgatca tcgccgaggt ggccaaggag cagggcgcgc tcacggtcgc ggtcgtcacc
     1381 aagcccttcg acttcgaggg caaggtgaag atgcgcctgg ccgaggaggg catcgagagg
     1441 ctcaggcagg ccgtcgatac gctgatcgtc atcccgaacc agcacctgct caaggtggtc
     1501 gagaagcgca cgccgatacg cgaggcgttc aagctcgccg acgacgtgct caggcagggc
     1561 gtacagggca tctccgacct gatcac
//
LOCUS       DAOOLN010000504         1585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_101507_length_1585_cov_3.058170, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000504 DAOOLN010000000
VERSION     DAOOLN010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101507_length_1585_cov_3.058170"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..912
                     /locus_tag="PLW80_07000"
     CDS             229..912
                     /locus_tag="PLW80_07000"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB66289.1"
                     /translation="MPHASVSAVSRDEALRLAREFATARHADTPPCHDFSHVERVLAL
                     AERLAAEEGADLFVVRMAALLHDIGRGLEPRIGPDPDRHEELSVELARPFLEAIGLEP
                     ALTERVLRAVLEHRHRRGREPSSPEAACLYDADKLDSLGAVGVARAYLWLGEHGRSVH
                     YPPESWAGVDPSNNATEHDSLQREWEIKLSGLKDRLYTASGRAIAAERHERMRRFLDE
                     LELEARGEA"
     gene            complement(939..1112)
                     /locus_tag="PLW80_07005"
     CDS             complement(939..1112)
                     /locus_tag="PLW80_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66290.1"
                     /translation="MFGSFPSFTFESPAQSGTIAIDTTFTGQNPAFFRLFAVDQDGLS
                     TTSNVVHCRVNNL"
BASE COUNT          260 a          529 c          518 g          278 t
ORIGIN      
        1 gctccccgtg gtacgaagct tgtgtatcgg gtgcggccgg tgcggccggt gcggccggtc
       61 ctggcaagcc gctccccgtg gtacgaagct tgtgtatcgg gtgcggccag tgcggccggt
      121 gcggccagtc cgggccagtc cgggccagtc cagtccaggc cggtctgggc cgatccgggc
      181 cggtcctacc cgctgacagc ccgcggccgc tcggtatatg ctcgggccat gccgcatgct
      241 tccgtaagcg ccgtctcccg ggacgaggcg ctcaggctgg cccgcgagtt cgcgacggcc
      301 aggcacgccg atacgccccc ctgccacgac ttctcgcacg tggagcgcgt gctggcgctg
      361 gccgagcgcc tggccgcgga ggaaggcgcc gacctcttcg tcgtcaggat ggcggccctg
      421 ctccacgaca taggccgcgg cctcgaaccg cggatcggcc ccgacccgga ccgccacgag
      481 gagctgtccg tcgagctggc gcggcccttc ctcgaggcta tcggcctcga gccggccctg
      541 accgagcgcg tcctccgggc ggtgctcgag caccgccatc gccgcggccg ggagccgtcg
      601 agccccgagg ccgcctgcct ctacgacgcg gacaagctcg actccctcgg cgccgtcggc
      661 gtggcgcgcg cctacctctg gctcggcgag cacggccgct cggtccatta cccgccggag
      721 agctgggccg gcgtcgatcc gtcgaacaac gccaccgagc acgactccct gcagcgcgag
      781 tgggagatca agctatccgg cctcaaggat cggctctata ccgcgagcgg ccgtgccatc
      841 gcggcggagc ggcacgagcg catgaggcgc ttcctcgacg agctcgagct ggaggcgcgc
      901 ggcgaggcct gagcgaatag cgcacctagt tcgagctact acagattgtt tactcggcag
      961 tggacgacgt tgctcgtcgt cgacaggccg tcttgatcta cggcgaacag cctgaagaac
     1021 gcggggttct gccccgtgaa ggtcgtatcg atagcgatcg tgccgctctg agccggcgac
     1081 tcaaatgtaa aggacggaaa acttccaaac acataaggat acgcatccgg accgaacacc
     1141 gacgagtcga gtatgagcgt tttgatagac ttcgtgctcg agtatgttat ttggatggtt
     1201 ccgctcactt cggttccgtt ccacgcgggc tgcgttatcg ttagtacggg agcgacagcg
     1261 ctcgtatcgg ccaattcgaa cgtggatttg aactctgccg gattaccgat accatcgtag
     1321 ccgatgacgc ttaccgaata cgtaccgaca gacggtgtcc atacgagcga agccgtatac
     1381 gttcccttgc cgtcgtgcgt aggactagcg agcggaacca gggtgccgtc tccgtcccgc
     1441 aatgtgaacg aggtccggtc gtagtcgacg cccgcgtccg atcccccgtc cgcgatacgg
     1501 acggttatgc gctcgaggct cgatacgccc gagaggttcc tgggctccac atcgatatag
     1561 ggtcccatgt tgtctatatt gaacc
//
LOCUS       DAOOLN010000505         1584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_101632_length_1584_cov_2.237410, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000505 DAOOLN010000000
VERSION     DAOOLN010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101632_length_1584_cov_2.237410"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PLW80_07010"
     CDS             complement(<1..720)
                     /locus_tag="PLW80_07010"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPB66291.1"
                     /translation="MEQKDYRLAAIMYTDIVGYSRMMERDEAGTLSLLHNHNALVSDI
                     AAKRHGTVIKTIGDAFLIDFRNTVEALQCAMEVQSAIYERNKQTDSQPLLLRIGLHLG
                     DIYFFENDALGEGINIASRLQSLARPGCVCFSQDVYNQVLNKIDFQAEKLGKVSLKNI
                     TKEIHAYEIASPNVEFDPDRDKPRVGFKPVAEESPAATKPLPGAPTPSDRGYGPEAAR
                     GVLDDIRKAILADTKTMGRRLT"
     gene            complement(767..1408)
                     /locus_tag="PLW80_07015"
     CDS             complement(767..1408)
                     /locus_tag="PLW80_07015"
                     /inference="COORDINATES: protein motif:HMM:NF037864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HPB66292.1"
                     /translation="MCQYASAAVVREMMVLAGEGLARWAFDRGLPFPYYSQEAPGSRD
                     DLPDGLAGEFAKRRLMKAGMAGVQPRAHQGLGVTMYAQATSPLRRYGDLLGHQQARAA
                     LAAEAGRTSYPVLPADELSMRLARAAAGAQAVRKAERQSELHWTLAWLLERPGWEGEA
                     VVVQTGNGDALLYLPEIGLETRVRASGHELNTALRVRFQQADISKLEARFSVI"
     gene            complement(1369..>1584)
                     /locus_tag="PLW80_07020"
     CDS             complement(1369..>1584)
                     /locus_tag="PLW80_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66293.1"
                     /translation="RVRRASYAEADVLLGSGALASLAKAAELRKAYRLRNGAVDIAIP
                     EVRVWVEDGEREYRPCASTHRPPSCAR"
BASE COUNT          250 a          502 c          525 g          307 t
ORIGIN      
        1 ggtcagcctg cgtcccatag tcttggtgtc ggcgaggatg gccttgcgta tgtcgtcgag
       61 gacgccgcgt gccgcctcgg ggccgtagcc gcggtctgac ggagtcggcg cgccaggtaa
      121 gggtttcgtg gcggccggcg attcctcggc gaccggctta aaaccgacgc gcggcttgtc
      181 cctgtccggg tcgaattcga cgttaggact cgcgatctcg taggcgtgga tctccttagt
      241 gatattcttg agcgacacct taccgagctt ctccgcctgg aagtctattt tgttgagcac
      301 ctggttatac acatcctggg agaagcatac gcaccccggg cgggcgagcg actgcaggcg
      361 actggctatg ttgatgcctt cgccgagggc gtcgttctcg aagaagtaga tgtcgccgag
      421 atgtaggcct atacggagta gcaacggctg cgaatcggtc tgcttgttgc gttcgtagat
      481 ggccgactgc acctccatcg cgcattgtag cgcttccacg gtattgcgga agtcgatcag
      541 gaaggcgtcg ccgatggtct tgatgacggt gccatgacgc ttggcggcta tgtccgatac
      601 gagggcgtta tggttgtgga gcaatgatag cgtgccggct tcgtcccgct ccatcatcct
      661 cgagtatccg acgatgtcgg tatacatgat cgccgcgagg cgatagtctt tttgttccat
      721 gctcaccgcg ctagcataga atagccgtag ccgcgggtca actgcatcat atgacgctga
      781 agcgggcctc gagcttcgaa atatcggcct gctggaagcg tacgcgtaga gccgtattca
      841 actcgtgccc cgaggcgcgc accctcgtct cgagacctat ctccggcagg tacaggagcg
      901 cgtcgccgtt cccggtctgg acgacgaccg cctcgccttc ccagcccggt cgctcgagta
      961 gccaagctag cgtccagtgc agttcgctct gccgctccgc cttgcggacc gcctgcgctc
     1021 cggccgccgc cctggccagg cgcatcgata gctcgtcggc cggtaggaca gggtaggagg
     1081 ttctgccggc ctcggcggcc agggcggccc tagcctgttg gtggccgagc aagtcgccgt
     1141 agcggcgtag tgggctcgtg gcctgagcgt acatcgtcac gccgagcccc tggtgggctc
     1201 gaggctggac gccggccatg cccgctttca tgaggcggcg cttggcgaac tcaccggcga
     1261 ggccgtcggg caggtcgtcg cgtgagcccg gcgcctcctg gctgtagtac gggaatggca
     1321 gcccgcggtc gaaggcccag cgggccaggc cctcgccggc gagcaccatc atctcgcgca
     1381 cgacggcggc cgatgcgtac tggcacacgg gcgatattct cgctcgccat cctcgaccca
     1441 gacgcgaacc tccgggatgg cgatgtccac ggcgccgtta cggagcctat aggccttgcg
     1501 cagctcggcc gccttcgcga gggacgcgag cgcgccggag cccaacagga catcggcctc
     1561 ggcgtacgaa gcccgcctga cccg
//
LOCUS       DAOOLN010000506         1583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_101708_length_1583_cov_2.473168, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000506 DAOOLN010000000
VERSION     DAOOLN010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101708_length_1583_cov_2.473168"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..1369)
                     /locus_tag="PLW80_07025"
     CDS             complement(35..1369)
                     /locus_tag="PLW80_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB66294.1"
                     /translation="MKEKKEGFRNYMGLTFLIGFGFFTMGLMDPLYDTYLPAFLGRYI
                     SSKTLVGAVMTLDNVLQLFLIPIVAVWSDRTRTRIGRRMPFILVMLPASAVFFGLIPW
                     SAGVSLGALITMIFLLNLFKTSVRGPVVALMPDTIPGEYRSEANGVINTMGGIGTIIG
                     TLALARLMDLDVVLPIIGRTKDMLPFPLAGLFVIVAVVILALFVRERNHDGEATEEHV
                     PVIASIKAVVAQKDKSALYILVSLFLWFSAHAGVVLFLGLYVRDVLGASSGQMALPQG
                     VAGISYALTAVPMGYVAHKYGRRTTIRACLIGLFCLTGLFTLLNLIGPSIGLTGAPAF
                     YLFMGLMLVYGVFWGSVITNSFPMLWQMSTFGNIGIFTGLYYTFSQTANILAPPITGL
                     IIDLTMMFKPAPEYQYTGIFAFACVLQVAAFLVMAKVRKGEPDDVADEPAKA"
     gene            complement(1366..>1583)
                     /locus_tag="PLW80_07030"
     CDS             complement(1366..>1583)
                     /locus_tag="PLW80_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HPB66295.1"
                     /translation="QSLAAAIELSNAFVGKEGASRVHGGGFAGTIQAYVPIDRVPKYS
                     EYMDARFGAGATTIVHIRPDGAGEANV"
BASE COUNT          300 a          492 c          537 g          254 t
ORIGIN      
        1 ggcgggggcc cgccggcctt acggccggct ccggctaggc cttggccggc tcgtcggcca
       61 cgtcgtccgg ctcgcccttg cgcaccttgg ccatcactaa gaacgccgcc acctgcagca
      121 cgcaggcgaa cgcgaagatg cccgtgtact ggtactcggg cgccggcttg aacatcatcg
      181 tcaggtcgat gatcaggccg gtgatgggcg gcgccaggat gttggccgtc tgcgagaagg
      241 tgtagtacag cccggtgaag atgccgatgt tcccgaaggt ggacatctgc cacagcatcg
      301 ggaaggagtt ggtgatcacc gagccccaga acacgccgta gacgagcatc aggcccatga
      361 acaggtagaa ggccggggca ccggtcagcc cgatcgacgg tccgatcagg ttgagtagcg
      421 tgaagaggcc ggtgaggcag aagaggccga tcaggcaggc gcgtatcgtc gtcctgcggc
      481 cgtacttatg ggccacatag cccatcggca ccgcggtcag ggcgtaggag ataccggcca
      541 cgccctgggg cagggccatc tggcccgagc tggcgccgag cacgtcgcgc acgtagaggc
      601 cgaggaacag cacgacgccg gcgtgggccg agaaccacaa gaacagggat acaaggatgt
      661 agagagcgct cttgtccttc tgcgcgacga ccgccttgat cgaggcgatc accggcacgt
      721 gctcctcggt cgcctcgccg tcgtggttgc gttcccggac gaacagcgcc aggatgacga
      781 cggcgacgat cacgaacagg ccggccagcg ggaagggtag catatccttg gtgcggccga
      841 tgatgggtag cacgacgtcg aggtccatca ggcgcgccag ggccagcgtg ccgatgatcg
      901 tgcctatgcc gcccatcgtg ttgatcacgc cgttcgcctc ggagcggtac tcgccgggga
      961 tggtgtcggg catcagcgcg acgacggggc cgcgcacgct cgtcttgaac aggttcagca
     1021 ggaatatcat cgtgatcagc gcgccgagcg atacgccggc ggaccacggt atcaggccga
     1081 agaacaccgc cgaggcgggc agcatcacga ggatgaacgg catgcgccgg cctatcctgg
     1141 tccgcgtacg gtcgctccat accgcgacga tcggtatcag gaacagctgc agcacgttgt
     1201 cgagcgtcat caccgcgccg acgagggtct tggacgagat gtacctgccg aggaaggccg
     1261 gcaggtaggt atcgtacagc ggatccatca ggcccatcgt gaagaacccg aatccgatca
     1321 ggaaggtcag gcccatgtag ttcctgaagc cctctttctt ttctttcata cgttcgcctc
     1381 tccggccccg tcgggcctga tatgaacaat ggtggtggcc ccggcgccga agcgcgcgtc
     1441 catgtactcg gagtacttgg ggacgcgatc gatcggcacg taggcctgga tggtgccggc
     1501 gaagccgccc ccgtgcaccc tggacgcgcc ttccttgccg acgaaggcat tcgacagctc
     1561 tatggccgcc gcgagcgact ggt
//
LOCUS       DAOOLN010000507         1583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_101719_length_1583_cov_2.096859, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000507 DAOOLN010000000
VERSION     DAOOLN010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101719_length_1583_cov_2.096859"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PLW80_07035"
     CDS             <1..249
                     /locus_tag="PLW80_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66296.1"
                     /translation="ATRYVLSRGGPAMIDWDPPACPLYLARLPALGEVVAALPQWQHG
                     SLAAYCEAEPTRALLSACAALDAAIEAHAHDPNRPREG"
     gene            253..933
                     /locus_tag="PLW80_07040"
     CDS             253..933
                     /locus_tag="PLW80_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66297.1"
                     /translation="MGLFGDLVGRVVMKYEADTKQAREAVRGLSAEQKKAAKEQAEAL
                     DKQTEKVKTWAGEWVKGAAIIGGTLLVAQNSLAKYRDETRLAAGAAGVDINRLSEAYD
                     GLRTRTELMTLAQAGHQGAWKLTTGQIELVVDGMRALEAKGYDSQKVFERFTEILRKG
                     KTEGIDEFGISLKDTGDKATDLRQLMRELGRQVVDVGGNLDKAGDSAQRALVKMEDGF
                     TRVRVAAG"
     gene            946..>1583
                     /locus_tag="PLW80_07045"
     CDS             946..>1583
                     /locus_tag="PLW80_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66298.1"
                     /translation="MLDYASAAGAGLGRLVFGELPTARDLARIAGNNDLAVDQYALTL
                     QMRRGHMSSGWKADAGDLAGSGRLFAAPSGQDVADAQAIDFFRRNLRRLRTVDELDKA
                     LAKLPSDWKSVDANLVDRVDRYRQEIARKTIDEMATGLKSALALAATGGGAKPSPRRS
                     GGRGARGEAVAGDTFLDEAIGYGRDFYAFAMSEAERAGEASRGRLGLDMIET"
BASE COUNT          269 a          543 c          555 g          216 t
ORIGIN      
        1 gcgacgcgct acgtcctgtc tcgcggcggc ccggccatga tcgattggga cccgccggca
       61 tgcccgctgt acctcgcgcg cctgcccgcg ctcggtgagg tcgtcgcggc cctgcctcag
      121 tggcagcacg gcagccttgc agcctactgc gaggccgagc cgacgcgtgc gctcctctcg
      181 gcgtgcgctg ccctcgacgc tgcgatcgag gcgcacgcgc acgaccccaa ccgcccgagg
      241 gagggctagc ccatgggact cttcggcgat ctcgtcggcc gcgtggtgat gaagtacgag
      301 gccgacacga agcaggcccg cgaggccgtg cgcggactgt cggctgagca gaagaaggcc
      361 gccaaggagc aagccgaggc gctggacaag cagaccgaga aggtcaagac gtgggccggc
      421 gagtgggtga agggcgccgc catcatcggc gggactctcc tcgtcgcgca gaacagcctc
      481 gccaagtacc gcgacgagac gcgactcgcc gcgggcgcag caggcgttga tatcaaccgc
      541 ctctccgagg cgtacgacgg actccgcacg cgcaccgagc tgatgacctt ggcgcaggcc
      601 gggcatcaag gcgcgtggaa gctgaccacc gggcagatcg agcttgtcgt cgacggcatg
      661 cgcgcgctcg aggcgaaggg ctacgactca cagaaggtct tcgagcggtt cacggagatc
      721 ctgcgcaagg gcaagaccga aggcatcgac gagttcggga tctcgctcaa ggacaccggc
      781 gacaaagcaa ccgacctgcg ccagctcatg cgcgagttgg gacgccaagt cgtcgacgtc
      841 ggcggcaact tagacaaggc cggcgactcg gcccagcgcg cgctcgtcaa gatggaggac
      901 ggattcaccc gcgtgcgcgt cgcggctggc taggccgtca gctccgtgct cgactatgcg
      961 tccgccgccg gggccggcct tggccgtctg gtcttcggtg agctgccgac cgcacgcgat
     1021 ctagcgcgta tcgccgggaa caacgatctt gccgtcgacc aatacgcgct cacgttgcag
     1081 atgcgacgcg gacacatgtc gtcgggatgg aaggccgacg ccggcgacct cgccggctcg
     1141 ggccgcctct tcgccgcgcc gtccggccag gacgtggccg acgcgcaggc catcgacttc
     1201 ttccgccgca accttcgccg cctccgcacc gtcgacgaac tagacaaggc gctagccaag
     1261 ctgccgagcg actggaagtc cgtcgacgct aacctggtcg accgcgtcga tcgctaccgc
     1321 caggagatcg cgcgcaagac gatcgacgag atggcgaccg gcttgaagtc ggcgcttgct
     1381 ctcgcggcga cggggggtgg cgcgaagccg tcgcctcgca ggtcgggtgg ccgcggcgcg
     1441 cgcggcgaag cggtcgccgg tgacacgttc ctcgacgagg ccatcggcta cgggcgcgac
     1501 ttctacgcct tcgcgatgag cgaagccgag cgcgccggcg aggcgtcgcg cgggcggctt
     1561 ggcctcgaca tgatcgaaac gca
//
LOCUS       DAOOLN010000508         1582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_101754_length_1582_cov_3.788474, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000508 DAOOLN010000000
VERSION     DAOOLN010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101754_length_1582_cov_3.788474"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            359..991
                     /locus_tag="PLW80_07050"
     CDS             359..991
                     /locus_tag="PLW80_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66299.1"
                     /translation="MLCLSAVWALGAYPIGGSPLAVVSLGMAAVAFVPAAVILASFLT
                     FFLLNRMTSSRLRGGITLIALASLTVVGVALLARFLGPSGAVAPSSLPGDYGPIAEWM
                     LEASTAPWPLFAAAYSSLAVYAASFWGLTRLSRSRPLIGAFIAPSAAIAAIRLLSLCL
                     SEPASAIFPLIGLDLPRLYGQAAIVTAGALALALFDLLFARKPSGGGRDA"
     gene            984..>1582
                     /locus_tag="PLW80_07055"
     CDS             984..>1582
                     /locus_tag="PLW80_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66300.1"
                     /translation="MPERRSSFLPVAIALLCIAFAVGFFVALVSIGRADVLYKFALSW
                     EAMRALIRLADWAPAILLVAAAIATESSDSDAGFSGAARGVLAPALALATVLSLFYLL
                     VLPVVEERSNRYESQSQLFADSLRAAETAYREGRLADAERALLAGAAIDQLDGRFVEL
                     SDRIRSEAARARAATIEDVDEAPDSGADAVPWRVANRFY"
BASE COUNT          246 a          504 c          485 g          347 t
ORIGIN      
        1 cgaaaactat gtgtatccgt ttcgtcctgt attaaatcgt ataccatagc ctcgaacgac
       61 acacggccct ttgtttaaaa tataaataca tgcccggaaa gcaattacag tcgagcgaat
      121 atggcttgca cgctgagcct cgctaggaag ctccaaaagg tcaaaaccga atacagctat
      181 ttttgtcgag ccgttgtgga taacttgtgt gctcttctgt atgaacaagg gaatccatag
      241 gcctatggac gccgcgtggc cattcggctt gtcaagccgt atggctcatg ctacagtttt
      301 aacgatgaaa aaaatcgcgg ctgcgattct caggtttttc ctcggattcg gcgtatcgat
      361 gctctgtctg tcggccgttt gggcgctcgg ggcgtatccc atcggcggat cgccgctcgc
      421 ggtcgtctcg ctagggatgg cagccgtcgc gttcgttcca gcggccgtca tcctggcctc
      481 gttccttacg ttcttcctat taaatcgcat gacatcctcg cggttacgtg ggggcatcac
      541 gttaatcgcg ctcgccagcc tgaccgtagt gggcgtagcc ctgctcgctc gcttcctagg
      601 accttccgga gcggtggcgc cgagctcgct gcctggagac tatggtccca tagccgagtg
      661 gatgctcgag gcgtccacgg cgccctggcc gctattcgcc gcggcttact cgtcgctcgc
      721 cgtctacgcc gcgtcgttct ggggacttac ccgcctatcc cgctcgaggc cgcttatcgg
      781 cgcctttata gctccgagcg ccgcgatagc ggcgattcgg ctcctgtcgc tctgcctgtc
      841 ggagccggcg agcgctatat tcccgctgat cggcctcgac ctgccgaggc tgtacggcca
      901 ggcggcgatc gttacggccg gcgctctcgc gctggccttg ttcgacctgc tgttcgcgag
      961 gaagccctca ggcggaggcc gcgatgcctg aacggaggtc gagcttcctg cccgtggcta
     1021 tcgcgctcct atgcatcgcc ttcgccgtag gcttcttcgt cgcgctggta tccataggac
     1081 gggccgacgt actgtataaa ttcgccctgt cgtgggaagc gatgcgggca ttgatccgcc
     1141 tggccgactg ggctccggcg atcctgctcg tcgccgcggc catcgcgacc gagtcgtcgg
     1201 actcggatgc cggtttctcc ggggcggccc gaggtgtgct ggcgccggcc ctcgccctgg
     1261 cgaccgtcct gtcgctattc tacctcctcg tgcttcccgt cgtagaggag cggtcaaacc
     1321 gatacgagag ccaaagccag ctgttcgccg attcgcttcg cgccgcggag acggcttacc
     1381 gggaggggcg cctggctgac gccgaacggg cgctcctggc cggagccgcg atcgaccagc
     1441 tcgacggtcg cttcgtcgag ctgagcgaca ggatcaggtc ggaagccgcg cgcgccaggg
     1501 cggcgaccat cgaggatgtg gacgaggccc ctgactccgg cgccgacgcc gtgccgtggc
     1561 gggttgccaa ccgcttctac tt
//
LOCUS       DAOOLN010000509         1581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_101887_length_1581_cov_2.865007, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000509 DAOOLN010000000
VERSION     DAOOLN010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101887_length_1581_cov_2.865007"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..265)
                     /gene="xseB"
                     /locus_tag="PLW80_07060"
     CDS             complement(32..265)
                     /gene="xseB"
                     /locus_tag="PLW80_07060"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPB66301.1"
                     /translation="MKSFEERLRRLEELSEKIREPDLALEDAVAAFEEGVKLSKGLEK
                     DLDRIEARVEILLNEPAPPDGKPELGLFDPVDQ"
     gene            complement(262..1464)
                     /gene="xseA"
                     /locus_tag="PLW80_07065"
     CDS             complement(262..1464)
                     /gene="xseA"
                     /locus_tag="PLW80_07065"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPB66302.1"
                     /translation="MNDDRALTVSELTGIVKELLEGSLPSVLVEGEISNWRPASSGHV
                     YFTLKDAGASLQAVMFKGRASRLDFKPSDGALVRARGALGVYAARGQYQLVVDTMQKA
                     GEGNILAMLERRKRQLAAEGLFDAERKPPLPAFPTRVAVVTSPTGAALRDILSVLGRR
                     NSGVRVTVLPAAVQGDQAPEAIVRQIEAANAFALGDVIIVGRGGGSLEDLLAFSDEAV
                     VRAVAASRIPVISAVGHEIDWALSDYAASLRAPTPSAAAELVSESAQAIADRVTVAAD
                     SLALGIRSRLERARGVVDSFSSEQLEMRLERALQPSRQRFDDAKEALVRGARDARLAA
                     RHRLSSADAVLRASDPLAILARGFAVVRKPGEARAVRSAAELAAGDGVSIQFSSGGAD
                     AAIVEVRT"
BASE COUNT          217 a          576 c          546 g          242 t
ORIGIN      
        1 cgaccgctcg ctgctcgcgt tcgtgctgcc gctactggtc gaccgggtcg aataggccga
       61 gctcgggctt gccgtccggc ggcgccggct cgttgagcag tatctccacc cgggcctcga
      121 tgcggtcgag gtccttctcg agccccttgg acagcttgac gccttcctcg aacgcggcga
      181 ccgcgtcctc gagggccagg tccggctcgc gtatcttctc cgacagctcc tcgagccttc
      241 tcagccgttc ctcgaaactc ttcatgtccg tacctccacg atggcggcgt cagcgccgcc
      301 cgacgagaac tgtatcgata cgccgtcccc ggccgcgagc tcggcggcgc tcctgacggc
      361 ccgggcctcg cccggcttcc tgaccaccgc gaagccgcgc gccaggatgg ccagcgggtc
      421 ggaggcgcgc agcacggcgt cggccgacga taggcggtgc ctcgcggcga ggcgggcgtc
      481 gcgggcgccg cgtaccagcg cctccttggc gtcgtcgaag cgctggcgcg acggctgcag
      541 cgctcgctcc aacctcatct ccagctgctc agacgagaac gagtcgacga cgccccgggc
      601 ccgctcgagc ctggatcgta tgccgagggc cagcgagtcc gccgcgaccg tcacccggtc
      661 agcgatggcc tgggcgctct ccgataccag ctcggcggcg gcgctcggcg tcggggccct
      721 gagggaggcg gcgtagtcgc tcagcgccca gtctatctcg tggcccacgg cgctgatcac
      781 cggtatcctc gaggccgcca cggcgcgcac gaccgcctcg tcggagaagg cgagcaggtc
      841 ctcgagggag ccgccgccgc ggccgacgat gatcacgtcg cctagggcga aggcgttggc
      901 cgcctcgatc tgcctgacga tggcctccgg cgcctggtcg ccctgcacgg ccgcgggcag
      961 cacggtgacg cggacgccgg agttgcggcg gccgagcacc gacaggatgt cgcggagagc
     1021 ggcgccggtc ggcgaggtga ccacggccac cctggtcggg aaggccggca gcggaggctt
     1081 gcgctcggcg tcgaacaggc cctcggcggc cagctgccgc ttccgcctct cgagcatcgc
     1141 caggatgttg ccctcgccgg ccttctgcat cgtatcgacg acgagctggt actggcctcg
     1201 cgccgcgtag acgccgagcg cgccgcgcgc cctgaccagg gccccgtccg atggcttgaa
     1261 gtcgagcctg gacgcgcgcc ccttgaacat caccgcctgg agcgacgcgc ctgcgtcctt
     1321 cagcgtaaaa tagacgtggc cggatgaggc gggtcgccag ttcgatatct cgccctcgac
     1381 cagtacgctc ggtagcgagc cctcgaggag ttccttgacg atgccggtga gctcgctcac
     1441 ggtcagcgct cggtcgtcgt tcatgccgac actctagccc ggcccggcgc ggcggcgcaa
     1501 gtccggcact gtcgcgcacg tccggtgcgg cggcgcacgt ccagtggcaa aagggtcgaa
     1561 tatcctgtat aacggatggc a
//
LOCUS       DAOOLN010000510         1578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_102165_length_1578_cov_3.518056, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000510 DAOOLN010000000
VERSION     DAOOLN010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102165_length_1578_cov_3.518056"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLW80_07070"
     CDS             <1..294
                     /locus_tag="PLW80_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HPB66303.1"
                     /translation="SAAHRLIQARLEVLLGNDDFQLETASPGVDRVIRSPAEYAIFVG
                     RGVRLFLDDEDVVEGRIASSDGEAVVVSTAGGDRSVAIASIRKGKLDYSQEGR"
     gene            298..>1578
                     /gene="nusA"
                     /locus_tag="PLW80_07075"
     CDS             298..>1578
                     /gene="nusA"
                     /locus_tag="PLW80_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPB66304.1"
                     /translation="MASDLAEAIRQLIYEKGISEDLVLKTIEDALLAAYKKRYGTAEN
                     AVVRFTGEYEGVEILAKRTIVDELDDPVLEISLDEAKELVDGAELGDILEIPIDPREF
                     DLQEVMLAKQTTRQSLREISRDTLYAEYKAKQGEIIIGYYQREKGGNIFVDLGRVEGF
                     FPKKFQSPRETYQPGERIKALIAEVSKNGPQIVLSRTHPEFVQKILELEVPEVYDKTI
                     EVFKIVREPGYRTKIAVYSKRDDVDPVGACVGLKGIRIQSIIRELEGEKIDVLKYEID
                     PRRFIKNALSPADVREVYIIDEARHTALAVVDESQLSVAIGKQGLNVRLANRLCDWSI
                     DVKTEEQFRELDLSSEARRAASSLFGDEEEILRLDELPGLDPGLVAALAGAGIEMIED
                     FLSRPEDELLAIDGVDPEGLSALRIHSPDPEKIAP"
BASE COUNT          326 a          488 c          487 g          277 t
ORIGIN      
        1 tccgcggcgc accgtctcat tcaggctcgt ctggaagtcc tgctcgggaa cgacgatttc
       61 cagctggaga ccgcctcccc aggcgtcgac cgcgtaattc gctctccggc cgagtatgcc
      121 atcttcgtcg gccgtggcgt tcgtctattc ctcgacgatg aggacgtcgt cgaggggcgt
      181 atcgcgtctt cggacggcga ggcagtcgtc gtctcgaccg cgggcggaga ccgttccgtc
      241 gctatcgcaa gcattcgaaa aggtaagctc gattattccc aggagggtag gtaagccatg
      301 gcctcagatt tggcagaagc gattcgccag ctcatatatg aaaagggtat ctcggaggat
      361 ctagtcctca agacgatcga ggacgcgttg ctggcggcgt acaagaagcg ctatggcacc
      421 gcggagaacg ccgtcgtccg cttcaccggc gagtacgagg gcgtcgagat tctcgccaag
      481 cgcaccatcg tcgacgaact ggacgatccg gtactcgaga tatcgctgga cgaggccaag
      541 gagctggtcg acggcgccga actcggggat atcctcgaga ttccgatcga cccgagggag
      601 ttcgacctgc aggaggtgat gctcgccaag cagacgaccc gtcagtcgct ccgcgagata
      661 tcccgcgaca ctctgtacgc cgagtacaag gccaagcagg gcgagatcat catcggctac
      721 taccagcgcg agaagggcgg gaacatcttc gtcgacctcg gcagggtcga gggcttcttt
      781 ccgaagaaat tccagtctcc gcgcgagacc taccagccag gagagcgcat caaggccctc
      841 atcgccgagg tcagcaagaa cggccctcag atcgtgctgt cccgtactca tcccgagttc
      901 gtccaaaaaa tactcgagct cgaagtgcct gaggtctacg acaagaccat agaggtcttc
      961 aagatagtgc gcgagccagg ctatcgtacc aagatagccg tgtactcgaa gcgggatgac
     1021 gtcgaccccg tcggcgcctg cgtcggcctc aagggcatac gcatccaaag catcatacgc
     1081 gagctcgagg gcgagaagat cgacgtgctc aagtacgaga tcgatccgcg ccgcttcatc
     1141 aagaacgcgc tgtcccccgc ggacgtgcgt gaggtctaca tcatcgacga ggctaggcat
     1201 accgccctcg ccgtcgtcga cgagagccag ttgtctgtcg ccatcggcaa gcagggcctg
     1261 aatgtacgcc ttgccaaccg gctctgcgac tggagcatcg acgtcaagac cgaggagcag
     1321 ttcagggagc tcgacctctc gagcgaggcc aggcgtgccg cctcgagcct gttcggcgac
     1381 gaggaggaga tcctccggct ggacgagctg ccgggcctcg accccggcct ggtcgccgcc
     1441 ctcgcgggcg ccgggataga gatgatcgag gacttccttt ccaggccgga agacgagctg
     1501 ctcgccatag acggcgtcga tccggaaggc ctctcggccc tgcggatcca ttctcctgac
     1561 ccggagaaga tagctcct
//
LOCUS       DAOOLN010000511         1578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_102169_length_1578_cov_3.399869, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000511 DAOOLN010000000
VERSION     DAOOLN010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102169_length_1578_cov_3.399869"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..>1578
                     /locus_tag="PLW80_07080"
     CDS             109..>1578
                     /locus_tag="PLW80_07080"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HPB66305.1"
                     /translation="MTNLYRIRFKPNRDLLTRGVKVENLFGDLAELGQYYAYADVDAV
                     PCLDAIDPTSLYLSWIIDLSTAAPVEDIRSVFIFVEDYAEIGIERLEVVDQTGKPSVP
                     RIGEILANRGALAPDDIDAIHRKQKPFGELAVESGKLSKPALEAALAEQRMAKAAAGE
                     RESRKESSNIRVRKEKLDYLIDAVGELVILEARLRQEARVAGATAFESIAENLGRLTS
                     DLRDATMNIRMVPLEESFSGFHRLVRDLASQLGKEIDLVISGANTEMDKNIIEALKDP
                     LVHIIRNSADHGIEAPELRAKLGKSRAGTIRIEARQQGSKVEIAISDDGAGLDLERIK
                     AKGVERGLIDATEKDPKRIMPLIFEPSFSTAARTTGVSGRGVGMDVVKSNLERLRGEI
                     AISSESGLGTRILLSIPLTLVIVDGLLVRVGDVMYVLALNQVEECVDVACSEVLGDGR
                     RGIINLRGKTIPIIDLRDAFGLDAADDAGLQRLVIVDAEG"
BASE COUNT          303 a          533 c          515 g          227 t
ORIGIN      
        1 cgatccgggt cgtcccccgc gaccacgaaa ccggccgctg aacctgccgc cgaaccggcc
       61 gtcgcgccga ccgtaggccg gggccgcgcc gacgtcgact ccgatggctt gacgaacctg
      121 taccgcatac ggttcaagcc gaaccgagac ctgctgacgc gcggcgtcaa ggtcgagaac
      181 ctgttcggcg acctcgccga gctcggtcag tactatgcat acgccgacgt cgacgcggtg
      241 ccgtgcctcg acgcgatcga tccgaccagc ctgtacctct catggatcat agacctgtcg
      301 acagccgccc ccgtcgagga tatacggtcg gtgttcatct tcgtcgagga ctacgccgaa
      361 atcgggatcg agaggctcga ggtcgtcgac cagaccggca agccgagcgt cccccgcatc
      421 ggagagatac tggcgaaccg cggcgccctg gccccggatg acatcgacgc catccaccgg
      481 aaacagaagc cgttcggaga actggccgtg gagagcggca agctgtcgaa accggccctg
      541 gaggccgccc tggcggagca acgcatggcc aaggcggcgg ccggcgagcg cgagtcgcgc
      601 aaggagtcgt cgaacatccg cgttcgcaag gagaagctcg actacctgat cgacgcggtc
      661 ggagagctgg tgattctcga agcccggcta cgccaggagg cgagggtcgc cggggctacc
      721 gccttcgaga gcatcgccga gaacctcggg cggctgacct ccgacctgcg cgacgccacg
      781 atgaacatac gcatggtacc gctcgaggag tcgttctcgg gcttccaccg cctggtacgc
      841 gatctagcct cgcagctagg caaggagatc gacctggtca tctccggcgc caacaccgag
      901 atggacaaga acatcatcga ggcgctcaaa gacccgctcg tgcacatcat ccgaaactcg
      961 gcggaccacg gcatcgaggc gccggagctg cgagccaagc tcgggaagag ccgtgccggc
     1021 acgatacgca tcgaggccag gcagcagggc tcgaaggtcg agatagccat ctcggacgac
     1081 ggcgccggtc tggatctcga gcggatcaag gccaagggtg tcgagcgcgg tctgatcgac
     1141 gcgacggaga aggaccccaa acgcatcatg ccgctcatct tcgagccgtc gttctcgacc
     1201 gccgccagga cgaccggcgt ctcgggccgc ggcgtcggca tggacgtggt caagagcaac
     1261 ctcgagcggc tacgcggcga gatagctatc agttccgagt cgggcctggg cacgcgcatc
     1321 ctcctgtcca taccgctcac cctcgtcatc gtcgacggcc tactcgtgcg ggtcggcgac
     1381 gtgatgtacg tgctggcgct caaccaggtg gaggaatgcg tcgacgtcgc atgctccgag
     1441 gtactcggcg acggtcggcg cggcatcatc aacctgcgcg gcaagaccat ccccatcatc
     1501 gacctgcgcg acgccttcgg cctggacgcg gccgacgacg cgggactcca gcgcctggtc
     1561 atcgtcgacg ccgagggc
//
LOCUS       DAOOLN010000512         1578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_102172_length_1578_cov_3.264609, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000512 DAOOLN010000000
VERSION     DAOOLN010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102172_length_1578_cov_3.264609"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            386..1303
                     /locus_tag="PLW80_07085"
     CDS             386..1303
                     /locus_tag="PLW80_07085"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPB66306.1"
                     /translation="MTTDGKRRLKGILLVNVATCAWATNIMIGRLVRDQIGPLTLTAT
                     RYVVASIVFAFVLRARPIQERRLGKDWLLLGAMSLTGVVLFAPTLYFGLSYTQAINGT
                     LINGIGPLLTAAFAAWLLREPFSGRQLFGSVLALGGVVVLLFGSASGPSKALSLNRGD
                     LLIVVAVTFWALYSVATRRVVRDRSPISATALSMFIGLPVLLVFAVLEHGLQPVAWTP
                     RLIGLVVYVGLVPAALGFLCWNAGVKLLGAGGAMVFYNTLPLYGVLFGFLLLGETLGL
                     AHLAGGALILGGGLVAAISKRASHLAVNK"
BASE COUNT          230 a          465 c          521 g          362 t
ORIGIN      
        1 ggctcgtctc tgcgtggatg gctataccgt gtcgttcccg ctatggaagt atactctcgg
       61 tgtgccgtta ttcgttgcga cagacgtgct attacgagac gttggcacgt cgcatgggcg
      121 cttctccggc acggacgggt gtacgctcgc gctttaaagc ctggccaacg gccttgagcc
      181 cgtcgctatc ggcgagctga cgatagtcct cgacgcgctc agtgtgttta gcgctatact
      241 cgcggcggcg ggctccctgg tcgaggggtt cgacggcgac gcgatagcga gttcggattc
      301 ggcgctcggt tctctgagct atccgccagg atgggctata ccgactacga ctcgccatag
      361 tacggtgcga cgaggaggaa tagcggtgac taccgatggg aagcggcgac tcaagggaat
      421 tctgctcgtt aacgtcgcga cctgcgcctg ggcgacgaat ataatgatcg gccggctcgt
      481 gcgagatcag atcggcccgc tgacgctgac cgctacgcgc tatgtcgtcg cctctatcgt
      541 cttcgcgttt gtcctacgcg cgagaccgat ccaggaacgg cgtctcggaa aggattggct
      601 cctgctcggg gcgatgtcgc tgaccggtgt cgtgttgttc gcccctaccc tatatttcgg
      661 cttatcctat acgcaggcga tcaatggtac cctgatcaac ggcatcggtc cgctcttgac
      721 cgcggcgttc gcggcctggt tgctgcgaga gccattttcc ggacggcagc tgttcgggtc
      781 ggtcctggcg ctcggcggcg tcgtggtcct gttgttcggc tcggcctcgg gtccaagcaa
      841 agctctgtcc ctcaaccgcg gcgacctgct gatcgtagtg gccgtgacgt tctgggcgct
      901 ctattcggta gcgacgcggc gcgtggttcg cgaccgttcg ccgatttctg ccacggccct
      961 gtcgatgttc atcggcttgc ccgtgctgct cgttttcgcg gtcctcgagc atgggcttca
     1021 gccggtggcc tggacgcctc gccttatcgg gctggtcgtg tacgtcgggc ttgtgccggc
     1081 ggcgctcgga ttcttgtgct ggaacgctgg tgtcaagctg cttggcgccg ggggcgccat
     1141 ggtgttctat aataccttac cgctctatgg cgtgctgttc ggtttcctgt tgctcggcga
     1201 gacccttggg ctcgcgcatc tcgccggagg cgcgttgata ctcggcggcg gactcgtcgc
     1261 ggccatttcg aagcgggcct cgcacttggc cgttaataaa taataatgtc attggattcg
     1321 gctcgacgtc atggctaatc gtttcattcg cggaatcgac gacggtgaca tcacgaccga
     1381 gggcgagctt cgctctgcct tcagggcgca ggcgctcgcg acgcatcccg acctgggggg
     1441 cggaaacgat ggatcggccg ccggaaacga tttcatgcgc gtccggacgg agtacgaggc
     1501 ggctgtacga tatcttgcta ggccggctgg agacccgagg ggagctacgt cgcgcagcgc
     1561 ggcgagcgcc ggtttcga
//
LOCUS       DAOOLN010000513         1578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_102187_length_1578_cov_2.879842, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000513 DAOOLN010000000
VERSION     DAOOLN010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102187_length_1578_cov_2.879842"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="PLW80_07090"
     CDS             complement(<1..1266)
                     /locus_tag="PLW80_07090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB66307.1"
                     /translation="MVSTGSRRILLVEDQAVIALNERRLLESLGYEVSLAYSGEQAIA
                     AFKADGSIDMAVMDIDLGDGIDGTEAAAEMLKAREVPVVFMSSHAEADIVEKTERVSS
                     YGYIEKNSGPKFVAATLRMAFRLFEARSSMQEANRRLEATLEALPDLLFELDEDGRYL
                     AVHCNDPGSLYAPIDGVIGTSVRDRLPPEAAETVLAALAEAKAGGLSRGRRYDLAFPE
                     GRRSFELSVSRMATGRSRRKFVMICRDITKQAKVEDELRRSKRQAERLLGISAQIIQV
                     VDLDGVVTVLNDSGHRLLGYEPPELIGQRWVDRCVPDESRPGVEAYLSSLFRSGADGI
                     QPHENDVVTKDGRRLRIHWQNSLLRDDEGRVVGVLSSGEDVTGYRELEERYRTIVDAS
                     PDNITVTDLRGRMLMFSPKARPMFGVENGE"
BASE COUNT          279 a          534 c          473 g          292 t
ORIGIN      
        1 ctcgccgttc tcgacgccga acatcggcct cgccttcggc gagaacatga gcatgcgccc
       61 ccgcaggtcc gtcaccgtga tgttgtccgg cgacgcatcg acgatggtcc ggtagcgctc
      121 ctcgagctcg cggtacccgg tgacgtcctc ccccgagctc agtacgccga cgacgcggcc
      181 ctcgtcgtcg cggagcaggc tgttctgcca atggatgcgc aggcgccggc cgtccttggt
      241 gaccacgtcg ttctcgtgcg gctggatgcc gtccgcgccg ctacggaaga gcgatgacag
      301 atacgcctcg accccgggcc gagactcgtc cggtacgcaa cggtctaccc agcgctggcc
      361 gatgagctca ggcggttcgt agccgagtag ccgatgcccg ctgtcgttca gcacggtcac
      421 gacaccgtcc aggtcgacga cctggatgat ctgcgccgat atgccgagaa ggcgctccgc
      481 ctgccgttta ctgcgtcgta gctcgtcctc gaccttcgcc tgcttcgtga tatccctgca
      541 gatcatgacg aacttgcgcc gcgaccgacc tgttgccatg cgcgacaccg acagctcgaa
      601 ggagcgccgg ccctccggga aggccaggtc gtagcgccgg ccgcgcgaca gcccgccggc
      661 cttggcctcg gccagggcag ccaggaccgt ctcggccgcc tcgggcggta gcctgtcgcg
      721 gaccgacgta ccgatgacgc cgtcgatcgg cgcgtacagg cttccggggt cgttgcaatg
      781 caccgccagg taccggccat cctcgtcgag ctcgaacagc aggtcaggca gggcctcgag
      841 cgtcgcctcg agccgccgat tggcctcctg catcgacgac ctggcctcga acagcctgaa
      901 agccatccgc agggtagcgg ccacgaactt gggccccgag ttcttctcta tataaccgta
      961 cgacgagacg cgctcggtct tctcgactat atccgcctcc gcgtggctag acatgaagac
     1021 caccggaacc tcgcgcgcct tgagcatctc ggccgcggcc tcggtaccgt cgataccatc
     1081 gcccaggtcg atatccatga cggccatgtc tatcgaaccg tcggccttga acgccgctat
     1141 agcttgttcg cccgaatacg ccagagagac ctcgtaaccg agggactcga gcaggcggcg
     1201 ctcgttcagc gcgataacgg cctgatcctc tacgagaaga atacgcctcg agccggttga
     1261 gaccatgcgt atagtgttcc acgaggcacg agctaatgca agtcttcttc ggtgttacga
     1321 accttatgta tgcgatcacg atgcttcgct agccgatact cgagactgcg ctagggcgat
     1381 gcgtcgacgc gattcgagct atcttgcacg ataccgggat atgcgattac aggatcaata
     1441 aggttcgtaa tactaggcac gggtcgcggg gccgccttcg cacactgcca cgcgcatcct
     1501 gcgatgcttt ttgccgctct cgcggcgagg gcgcttcggg ttcaagtccc cctctgggct
     1561 tataaaaaaa gcgagacc
//
LOCUS       DAOOLN010000514         1576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_102368_length_1576_cov_3.335963, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000514 DAOOLN010000000
VERSION     DAOOLN010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102368_length_1576_cov_3.335963"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="PLW80_07095"
     CDS             <1..686
                     /locus_tag="PLW80_07095"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPB66308.1"
                     /translation="CTPWGADEKSSLAALRKTTAWAARSKARWLETRGDFQGKLFGIV
                     QGNFYKELRARSAEEILALDTPGVAIGGLSVGEPFEVYADLLAYTAALLPEDKPRYVM
                     GIGTPEYILEAVQNGVDMFDCVFPTRTARNGLLFTAFGPISIKQARYTEDMGPADPDC
                     SCKVCREYSRAYLRHLYKNDEILFSMLASYHNLAFLHELVRRVRYSIEEGNFVEFKRE
                     FLSRYRSKL"
     gene            695..>1576
                     /locus_tag="PLW80_07100"
     CDS             695..>1576
                     /locus_tag="PLW80_07100"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPB66309.1"
                     /translation="MKRLSLLAVALLAVVVFAGADDTGRTESEKRAATIRYGIESQVV
                     ELLSTLKTEKDDSLKAEILDAFDASTSTKLKASILEYLAALELGDAEARAARIVAERD
                     SNAEGVVTAAFSYLRALKLRAALDEAAEIVADDEGRYLQAAIRYIGAAGTEKQAAALR
                     AAYEADGASQATKEAVVQALGALRSVESFELLSSVASSEESSKAIRMYACSALGELGD
                     DRAVPVLVGASVSSDPNVRAAAVEALGTYSGADALAAVRQALRDAHALPRIAAAKASG
                     ESRDAEALPYLEYKASYD"
BASE COUNT          273 a          512 c          528 g          263 t
ORIGIN      
        1 tctgcacgcc ctggggggcg gacgagaaga gctccctggc ggcgctccgc aagacgaccg
       61 cctgggccgc caggtccaag gcgcgctggc tggaaacgcg cggcgacttc cagggcaagc
      121 tgttcggcat cgtgcaaggt aacttttaca aggagctgcg cgccaggagc gccgaggaga
      181 tactggccct cgacacgccc ggcgtggcca tcggggggct gtccgtaggc gagcccttcg
      241 aggtctacgc cgacctgttg gcctataccg cggcgctcct gccggaggac aagccgcgct
      301 atgtcatggg catcgggaca cctgagtaca ttctggaggc ggttcagaac ggggtcgata
      361 tgttcgactg cgtcttcccg acgcggaccg cgaggaacgg cctcctgttc accgccttcg
      421 gcccgatctc gatcaagcag gcccgctaca ccgaggatat ggggcctgcc gaccccgact
      481 gcagctgtaa ggtctgcagg gaatactcgc gcgcctacct tcgccacctg tacaagaacg
      541 acgagatact cttctccatg ctcgccagct accataatct ggctttcctg catgaattgg
      601 tccgccgcgt ccgatacagt atagaggagg ggaacttcgt cgagttcaag cgggagttcc
      661 tgtccagata ccgttcgaag ctgtgagggc ccgcatgaaa cgattatcgc tcctggccgt
      721 cgcccttctg gccgtcgtcg tgttcgccgg cgcggacgat accggccgca ccgaatcgga
      781 gaagcgcgcg gcgacgatac gctacggcat cgagtcccag gtcgtcgagc tgctctcgac
      841 gctcaagacc gagaaggacg actcgctcaa ggccgagatc ctcgacgcgt tcgacgcgtc
      901 caccagtacg aagctcaagg cctcgatcct cgaatatctg gcggccctgg agttgggcga
      961 cgcggaggcg cgtgcggcca ggattgtcgc cgagcgtgac tcgaacgccg agggcgtcgt
     1021 cacggcggct ttctcctacc tgagggcgct gaaactgagg gcggccctcg acgaggccgc
     1081 ggagatagtc gccgacgacg aggggcgcta cctccaggcg gcgatcaggt atataggggc
     1141 cgccggcact gaaaagcagg ccgcggccct gcgagccgcc tatgaggccg acggcgcgag
     1201 ccaggcgacg aaggaagcgg tcgtacaggc gctcggcgcg ctacggtcgg tcgagtcgtt
     1261 cgagctgctc tcgtccgtcg cgtcgtcgga ggagtcgtcg aaggccatta ggatgtacgc
     1321 ctgctccgcc cttggcgagc tcggtgacga tagggccgtg ccggtactcg tcggcgcgtc
     1381 cgtttcgagc gatccgaacg tccgcgcggc cgcggtcgag gcgctcggta cgtattcggg
     1441 cgccgatgcg ctggcggccg tcaggcaggc cttgcgcgac gcccacgcct tgccacgcat
     1501 cgccgcagcg aaggcctccg gtgaatcccg cgacgccgag gccctgccat accttgagta
     1561 caaggcttcc tacgac
//
LOCUS       DAOOLN010000515         1575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_102485_length_1575_cov_2.665789, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000515 DAOOLN010000000
VERSION     DAOOLN010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102485_length_1575_cov_2.665789"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PLW80_07105"
     CDS             <1..509
                     /locus_tag="PLW80_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HPB66310.1"
                     /translation="VEVLICPDEIDDIVMPMVGKYGDLELKAVNRSGAEKEFEDKLDE
                     SKAKDLAPVIERVKKALGYEVKDVRLSARLSEGPSCIVVDADDPSIQLQQMMKSMGRG
                     GDFEVKPILEINGDHALVARLAASDDEALASDVARVLLDQALLVEGVALKDPADFVAR
                     INRLIADV"
     gene            complement(883..>1575)
                     /locus_tag="PLW80_07110"
     CDS             complement(883..>1575)
                     /locus_tag="PLW80_07110"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB66311.1"
                     /translation="RDLTIGTIPATIYGAGSGRAYLYVHGRRGSKADAEGFASIATAR
                     GYDVIAADLPGHGGRTADPTPCDPSHGSEELLSVYGRAAGLYPELSLFACSLGAYFSL
                     LAFRDVSFRRCLFLSPLLDMERLIRNMMGWAGVDESRLREEGEIVTGFGETLSWNYYR
                     YAVEHPVDRWPSQTSILYGEHDDITDRATLDAFIARFGCDVTVEPGGEHYFHTPGQLD
                     ALRAWTSSRVIP"
BASE COUNT          287 a          494 c          541 g          253 t
ORIGIN      
        1 gcgtggaggt gttgatctgc cccgacgaga tcgacgacat cgtgatgcct atggtcggca
       61 agtacggcga cctcgagctc aaggccgtca accgctccgg cgccgagaag gagttcgagg
      121 acaagctcga cgagagcaag gctaaagacc tggcccccgt gatcgagcgc gtcaagaagg
      181 ccctcggcta cgaggtcaag gacgtgcgcc tatcggcccg cctgtccgag ggcccgtcgt
      241 gtatcgtcgt cgacgccgac gacccgagca tccagctcca gcagatgatg aagagcatgg
      301 gacgcggcgg cgacttcgaa gtcaagccga tactcgagat caacggcgac cacgcgctcg
      361 tcgcccgcct ggccgcgtcc gacgacgagg ccctggcctc cgacgtggcc cgcgtgctgc
      421 tcgaccaggc cctgctcgtc gagggcgtcg ccctcaagga ccccgccgac ttcgtggcgc
      481 gcatcaatcg cttgatcgcg gacgtgtaag cgcgccaggc gcgctacgcg ccgcgatcaa
      541 gtagggtaag accgcatcgc cgacgtgata cgcgcgccat ggagggcgcg cccgacaacg
      601 gcccgtccgc aggacgggaa cgtcacggat gacgacgcgg cgatgcgggg aatttcattc
      661 aatcggacga gccgctgcgc ggttcgccgc ttaacgtaga gagaatatag ggcgttcaag
      721 cgggcgagtc gcggggcggc tcgccgcttg cttttagtag ttcctcgaag cttgtggtgc
      781 tttctctatt accaccatgc agggtagcgc cgcgaagccc gtgctacgcg ctatagtgtt
      841 tggacgagtc ggctcacaag cttcgaagac taagcttgcc gattacggta tgacgcgcga
      901 cgaggtccac gcccggagcg cgtcgagttg cccgggggta tggaagtaat gctcgccgcc
      961 cggctcgacg gtcacgtcgc agccgaaacg ggcgatgaag gcgtcgagcg tggctcgatc
     1021 ggtgatatcg tcgtgctcgc cgtacaggat cgaggtctgg ctcggccacc ggtcgacggg
     1081 atgctcgacg gcgtaacggt aataattcca agacaaggtc tctccgaagc cggtaacgat
     1141 ctcgccctcc tcgcggaggc gggactcgtc gacgcccgcc cagcccatca tgttccgtat
     1201 cagccgctcc atatcgagga gcggcgacag gaagaggcag cgccggaagg atacgtctcg
     1261 gaaggcgagc aggctgaagt acgcgccgag gctgcaggcg aagagcgaaa gctcgggata
     1321 caggccggcg gcgcgcccgt atacggagag cagctcttcc gatccgtggc tggggtcgca
     1381 tggcgtcggg tcggccgtcc gtccgccgtg gccaggcagg tcggccgcga tgacgtcgta
     1441 gccccgggcg gtcgctatcg aggcgaagcc ctcggcgtcc gccttgctgc cgcgccggcc
     1501 gtgcacgtat aggtaggcgc gtccggaacc ggcgccgtag atcgttgccg ggatagtccc
     1561 gatagtcagg tctcg
//
LOCUS       DAOOLN010000516         1573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_102662_length_1573_cov_2.915679, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000516 DAOOLN010000000
VERSION     DAOOLN010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102662_length_1573_cov_2.915679"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="PLW80_07115"
     CDS             complement(<1..725)
                     /locus_tag="PLW80_07115"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HPB66312.1"
                     /translation="MDRGYVALVLNAHLPFVRQPDYPSFLEERWLFESLSETYLPLLR
                     VFARLEADRIPWKLNLAVSPTLEAMLGDSMLQQRYVDWLGAQIELAEREVARCADDAA
                     FRPLAAMYHELYTKNREDFSVLYAGNIVGALDFYFKKGRIDLMTSGATNAFMPIYRDY
                     PEAIAAQIEAAVVSFRAAFGRSPSGFWMPQLGWYPGVEEPLSAYGLQYTVVSTRGALL
                     GDPTPRMGSYAPVSCPNGFTAGAS"
     gene            complement(738..1442)
                     /locus_tag="PLW80_07120"
     CDS             complement(738..1442)
                     /locus_tag="PLW80_07120"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HPB66313.1"
                     /translation="MNVERLNGLSIDALYALADKMGLDLPIELERVFVVEALLEAFEE
                     DSEERKASGDAAVHIEEKKYSGSELDEIDASLDAAPCIESRYNETVVHVIARDPEWAF
                     VFWDVKDDAFDELRANPAFSCLFLRVVKESVPEGKTAGSFEVVVGEDDDRWYLHLPDE
                     GCSYHVDLYARLGNRSRLLAHSAVVRTPRALMADSLAKLDADAATLATLSGLASLEIA
                     PAPEHHPSRILGGSDE"
BASE COUNT          290 a          503 c          512 g          268 t
ORIGIN      
        1 gacgccccgg ccgtgaagcc gttcgggcag gaaaccggcg cgtacgagcc catccgcggc
       61 gtaggatccc cgagcagggc cccccgggtc gagacaacgg tgtactggag gccgtacgcg
      121 ctcagcggct cctccacgcc agggtaccag ccgagctgag gcatccagaa gccggacgga
      181 gagcggccga acgcggcccg gaacgatacg accgcggcct cgatctgggc agcgatggcc
      241 tcaggatagt ccctatagat cggcatgaag gcgttcgtgg cgccgctggt catcaggtcg
      301 atacgcccct tcttgaagta gaagtcgagg gcgccgacga tgtttccggc atagagtacg
      361 gagaagtcct cgcggttctt cgtgtacagc tcatggtaca tggccgccaa cggcctgaag
      421 gcggcgtcgt cggcgcatcg cgccacttcc ctctcggcca gctctatctg cgcgccgagc
      481 cagtcgacgt agcgctgctg cagcatggag tcgccgagca tagcctcgag ggtcggcgaa
      541 accgccaggt tgagcttcca tgggattctg tcggcctcga gcctggcgaa gactcgcagg
      601 agcggcaggt aggtctccga cagcgactcg aacaaccagc gttcctctag aaagctcggg
      661 taatcgggtt gccgaacgaa cggcaggtgt gcgtttagaa cgagcgctac gtatccgcgg
      721 tccacggggc tcctccgtta ttcgtcggac ccgccgagga tgcgggaagg atggtgctcc
      781 ggcgctggcg ctatctcgag gctagccaat ccggacagcg tagccagcgt cgcggcgtcg
      841 gcgtcgagct tggcgagtga atcggccatc aacgccctcg gcgtcctgac gacggccgag
      901 tgggccagca atcgcgagcg attcccgaga cgggcgtaca gatcgacgtg gtacgagcaa
      961 ccctcgtcgg gcagatgaag ataccagcgg tcgtcgtctt cacccacgac cacctcgaac
     1021 gagccagcgg tcttgccctc ggggaccgat tccttgacga cgcggaggaa caggcacgag
     1081 aacgcaggat tggcgcgcaa ctcgtcgaac gcgtcgtcct tcacgtccca gaacacgaag
     1141 gcccactcgg gatcccgagc tatcacgtgg acgacagtct cgttgtagcg gctctcgatg
     1201 caaggggcgg cgtcgaggga tgcgtcgatc tcgtcgagtt cggagccgga atacttcttt
     1261 tcctcgatat ggaccgctgc gtcgccggaa gccttccgct cctcgctgtc ttcctcgaat
     1321 gcctcgagca gggcctcgac gacgaagacg cgttccaact cgatcggtaa atcgagcccc
     1381 atcttgtccg cgagggcgta gagggcgtca atcgataatc cgttcagccg ttcgacgttc
     1441 accgtcgcat catccgagaa atggcctcgc cgtgtcaagc catcgggcga aacgcatcct
     1501 cagggggcct attcaccgtc gcgcggctcg tcgccgcccc taggggacgg ctctgcggcg
     1561 ccggtcggcg gct
//
LOCUS       DAOOLN010000517         1571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_102846_length_1571_cov_3.623351, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000517 DAOOLN010000000
VERSION     DAOOLN010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102846_length_1571_cov_3.623351"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLW80_07125"
     CDS             <1..206
                     /locus_tag="PLW80_07125"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HPB66314.1"
                     /translation="DAEDEAFVARFIAGLGDAEREMLHLAYAEGLTYAEIAATVGAPL
                     GTVKWRLASVKRRLKAAYEREYL"
     gene            203..574
                     /locus_tag="PLW80_07130"
     CDS             203..574
                     /locus_tag="PLW80_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66315.1"
                     /translation="MTGKDVDRLLGAYYARRLEKGDGLYGKGDGLARRVGRGERLKPV
                     DCIRADAARADAARADGRLSAVLAAAALVMMLGFGVLGSPSGDESGARNGLVASMSLD
                     LAGSRFEEAVIAIGDSWPRKN"
     gene            609..>1571
                     /locus_tag="PLW80_07135"
     CDS             609..>1571
                     /locus_tag="PLW80_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66316.1"
                     /translation="MRLRFVSGVVVSACAVACAVYLSGGRVVPGYVALDLALITLLPL
                     GAAMAAFGPRAVGRAFTVTTDSIAAGASERRDAALRDAALRDADVMLRWLARATWLAM
                     AAGFLLIQIWMMYDLQDPSALFDNLALSLEPPILGFFVIYCVYRPLRHAVLNGLERET
                     PEAGAAPARGASPIQARTRWSLARWAAGIVVMASVLAAWNLVAYGRIGRFVDLAPLSL
                     ALFLPLGQLLAGPGPNGLRSAFAALSTDDPSALRSARRIFAFNAGSFAVAAALAFSLG
                     AAYFLEAIGDRMRYGPTLALAELGLLQSAVLALVLALPPLAAADR"
BASE COUNT          204 a          550 c          541 g          276 t
ORIGIN      
        1 tcgacgccga ggacgaggcc ttcgtcgcgc gcttcatagc ggggctcggg gacgccgagc
       61 gcgagatgct gcacctggcc tatgccgagg gtttgacgta cgcggagata gcggccaccg
      121 tcggcgctcc gctcggcacg gtcaagtggc ggctggcgtc ggtcaagcgt cggctcaagg
      181 ccgcctatga acgggagtac ctatgactgg aaaggacgtc gaccgcctgc tcggcgcgta
      241 ctacgcgcgg aggctcgaga agggggacgg gctctatgga aagggcgacg gactggcccg
      301 gcgcgtcggc cgcggagagc gcctgaagcc ggtagactgt attcgcgccg acgctgcgcg
      361 cgccgacgcc gcgcgcgccg atggccggct gtcggccgtc cttgccgcgg cggcgctggt
      421 catgatgcta ggcttcggcg ttctcggctc cccgagcggt gacgagagcg gcgcgagaaa
      481 cggcctggtc gcgtcgatga gcctcgacct ggccggttcg cgtttcgagg aggccgtcat
      541 cgccataggc gatagctggc cgcgcaagaa ctgatagcgc tttcttaaag cgtgtgatgg
      601 aggaaaacat gagactgaga ttcgtttccg gcgtcgtcgt atcggcctgc gccgtcgcct
      661 gcgccgtgta cctgtccggt ggacgcgtgg ttcccggcta cgtggccctc gacctggcct
      721 tgatcacgct cctgcctctc ggcgccgcca tggcggcctt cgggcctcga gccgtaggaa
      781 gggccttcac cgtcactacc gactcgatag ctgcgggcgc cagcgagaga cgcgacgcgg
      841 ccctgcgcga cgcggccctg cgcgacgcgg acgtcatgct gcgctggctg gcccgggcga
      901 cctggctcgc gatggccgcc ggcttcctgc tcatccagat atggatgatg tacgatcttc
      961 aggacccgtc cgctctgttc gacaacctgg ccctgagcct cgagccgcct atcctgggtt
     1021 tcttcgtgat ctactgcgtg taccggccgc tacgccacgc ggtactgaac ggcctcgaac
     1081 gcgaaacgcc cgaggccggc gcggcgcccg cacggggcgc gtcgccgatt caagcccgga
     1141 cgcggtggag cctcgcacgc tgggcggcgg ggatcgtcgt catggcctct gtcctagcgg
     1201 cctggaacct cgtggcctat ggcaggatag gccgcttcgt cgatctggcg cctctgtccc
     1261 tggccttatt tctaccgctc ggccagctgc tcgcggggcc aggcccgaac ggtctgcgct
     1321 cggccttcgc ggccctgagc acggacgacc cctccgccct gcgttcggcg cgccgcatct
     1381 tcgctttcaa cgccggatcc ttcgccgtcg ccgcggcgct cgcgttctcg ctcggggccg
     1441 cgtatttcct cgaggccata ggcgaccgca tgcgctacgg ccccacgctg gccttggccg
     1501 agctcggcct gctccagtcg gccgtcctgg ccctcgtcct ggccctgccg cctctggccg
     1561 ccgccgaccg a
//
LOCUS       DAOOLN010000518         1571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_102859_length_1571_cov_2.970317, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000518 DAOOLN010000000
VERSION     DAOOLN010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102859_length_1571_cov_2.970317"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PLW80_07140"
     CDS             <1..377
                     /locus_tag="PLW80_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPB66317.1"
                     /translation="VGRGWSSLSSYYGTALWDNWVEFRMPVVPSVLSLDGFLSAAMLS
                     TESGLLDVAGVDSGSTAYGGTLSELGLNNFAFSAGVGLRFIILQFPFRIYFAKKFIYD
                     DGFAWSSDDDWQFVLSVTTSLD"
     gene            400..930
                     /locus_tag="PLW80_07145"
     CDS             400..930
                     /locus_tag="PLW80_07145"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPB66318.1"
                     /translation="MKKTAIIGLALFALCAQAFGQQITRVGVIDLQKVYTTYYKDSQA
                     VRALEEEKARVNEEIKRLSEEIKELQKRRLGLLSSADAAALKTFDEELYRKAQFLSDF
                     VKIKQADLDAKAESMTKGDAFVQMLYRTVQAISEAEGFSLVISSRNASDVGSSVIWFS
                     PMIDITDKVIQALLGA"
     gene            complement(941..1432)
                     /locus_tag="PLW80_07150"
     CDS             complement(941..1432)
                     /locus_tag="PLW80_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPB66319.1"
                     /translation="MEEVLYIKETNNRVYIRAQGHITANACPELKARVFDRLDAKPAV
                     EDVIIDLSSCEYMDSTFMGLIVGFNKRFMRFSGRPIRLAGLSETCMKLLKTIGIVRLV
                     TIEKEPPAFPEPLERLGAEAKAGAGLVLRAHEELIELSEENERRFSALRDALKGVAGA
                     DQD"
BASE COUNT          290 a          493 c          483 g          305 t
ORIGIN      
        1 tcgtcggccg cggctggtcg tcgctctcct cgtactacgg caccgcgctc tgggataact
       61 gggtcgagtt ccggatgccg gtcgtgccga gcgtgctgtc gctcgacggc ttcctgagcg
      121 cggcgatgct gtcgaccgag agcggcctcc tcgacgttgc tggtgtcgac tcgggctcca
      181 cggcctacgg cgggaccctg tcggagctcg gtctcaacaa cttcgcgttc agcgccggcg
      241 tcggcctgcg cttcatcatc ctgcagttcc cgttcaggat ttacttcgcc aagaaattta
      301 tctacgacga cggcttcgcc tggtcgtccg acgacgactg gcagttcgtc ctcagcgtga
      361 cgacgagcct cgattgacgc gcatagcggg gggtacgata tgaagaagac ggcgatcatc
      421 ggcctggcgc tattcgcgct ctgcgcccag gccttcggcc agcagatcac gcgtgtgggc
      481 gtgatagacc ttcagaaggt ctatacgacc tactataagg actcgcaggc ggtacgcgcg
      541 ctggaggagg agaaggccag ggtcaacgag gagatcaagc gcctgagcga ggagatcaag
      601 gagctgcaga agcgtaggct cgggctcctg tcgtccgccg acgcggccgc gctcaagacc
      661 ttcgacgagg agctgtaccg caaggcccag ttcctgtctg acttcgtcaa gatcaagcaa
      721 gctgacctcg acgccaaggc cgagagcatg accaagggcg acgcgttcgt gcagatgctc
      781 tataggaccg tgcaggccat ctcggaggcc gagggtttct cgctggtcat tagctcaagg
      841 aacgcgtccg acgtcggctc gtcggtgatc tggttcagcc ctatgataga catcaccgac
      901 aaggtgatac aggcgctcct aggcgcttga gcggcggcct tcagtcctga tccgcccccg
      961 cgacgccctt gagcgcgtcg cggagcgcgg agaatcggcg ctcgttctcc tcggatagct
     1021 caatcagctc ctcgtgggcc ctgagcacga ggccggcgcc cgccttggcc tcggcgccaa
     1081 gcctctcgag cggctcgggg aaggccggcg gctccttctc tatcgtcacg agcctgacga
     1141 tgcctatcgt cttgagtagc ttcatgcagg tctcgctcag gcccgcgagc cgtatcggcc
     1201 gccccgagaa gcgcatgaag cgcttattga agccgacgat cagtcccata aaggtcgagt
     1261 ccatgtactc gcacgacgaa aggtcgatga tcacatcctc gaccgccggc ttggcgtcga
     1321 gtcggtcgaa cacgcgcgcc ttgagctccg ggcaggcgtt ggccgttatg tggccctgag
     1381 cgcgaatata gacgcggttg ttcgtctcct tgatgtaaag gacctcttcc acgacgacct
     1441 cgcaacgatg aagatggcgc tgcctctcgc ctaacagaat cgcctcgatt gtatcctagt
     1501 ccaagttacc ctataatgca acgccatggc cgaacaggta ccgctcctcg agcaatacga
     1561 tcgcatcaag g
//
LOCUS       DAOOLN010000519         1569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_103076_length_1569_cov_2.883091, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000519 DAOOLN010000000
VERSION     DAOOLN010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103076_length_1569_cov_2.883091"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PLW80_07155"
     CDS             complement(<1..348)
                     /locus_tag="PLW80_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66320.1"
                     /translation="MHPTRTRAAALAACLASLALASSCQQFFTTSLAPFLARDSYTIP
                     ADLPVEDAIRLLEQAMAQGDTSAAAALVTPLLAAATAAAEADPASAAYQQAASALLDA
                     AVLSSGVSSAMTTL"
     gene            complement(402..1547)
                     /locus_tag="PLW80_07160"
     CDS             complement(402..1547)
                     /locus_tag="PLW80_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66321.1"
                     /translation="MKRVIVLAMIALLVGGAAFAQTTLSNFYPKDARSMAMGGAFVAM
                     SDGFQSLYGNPAAFADKKAELTLLSMTPWLYLSPTTENIDAVSGLVSGEGDEQDMFNT
                     LTGLVTKNGFGAGASFGLGWVGKGLGLGLVGGADAYLSGKTLLGATGSVDGQMNAVIG
                     IGVPLNLGPFRLQVGGDVRPYLRMTGDIVGTQLIGMMAGGDDFDPMILEVDVGFGLAV
                     DLGATLELGKLVSVGLAIRDLSTKQTYARSTIGEVLESEGLPSGTEATYAVMPNITVG
                     AAVTPIPAFLRPLIDVTVIAELQDPIKVIRDNSSMWNLFHLGAEAETLGGLIAVRAGL
                     NKGWLSFGAGLDLLIMEVNVALFTEELGPYPGDRGRTGVSAEVAIRF"
BASE COUNT          251 a          494 c          567 g          257 t
ORIGIN      
        1 gagcgtggtc atcgcggagc taacgcccga cgacagcacg gccgcgtcga gcagggccga
       61 ggccgcctgt tggtaggccg cgctcgccgg gtcggcttcc gcagcggccg tagcggcggc
      121 caggagtggc gtgacgaggg cggccgcggc cgacgtgtcg ccctgggcca tcgcctgttc
      181 gagcaagcgg atggcgtcct cgacgggcag gtcggccggt atggtatacg aatccctggc
      241 caggaagggg gccagggacg tggtgaagaa ttgctggcag gatgaggcga gcgcgaggct
      301 cgcgaggcag gcggccaggg ccgccgccct cgttctagtc ggatgcatgg tcgccgctcc
      361 tttcgtcgta gtactagtat cggcgcggcg accggttttc atcagaaccg tatggccacc
      421 tcggccgaga cgccggtccg accgcggtct cccggatacg gcccgagctc ctcggtgaac
      481 agcgcgacgt tgacctccat gatcaggagg tcgagtccgg cgccgaagct cagccagccc
      541 ttattgaggc cggctcggac ggcgatgagc ccgccgaggg tctcggcctc ggcgccgagg
      601 tggaacaggt tccacatcga ggagttgtca cggatcacct tgatcgggtc ctggagctcg
      661 gcgataacgg tcacgtcgat cagcggccgg aggaaggccg ggatgggcgt gacggcggcg
      721 ccgacggtga tgttcggcat caccgcgtag gtcgcctcgg taccggaggg cagtccctcc
      781 gactcgagta cctcgccgat ggtcgaacgg gcgtaggtct gcttggtcga caggtcgcgg
      841 atcgcgaggc ctaccgatac cagcttgccg agctcgaggg tagcgccgag gtcgaccgcg
      901 aggccgaagc cgacgtctac ctcaaggatc atcgggtcga aatcatcgcc gccggccatc
      961 atgccgatca gctgggtacc gacgatgtcg ccggtcatgc gcaggtaggg tcggacgtcg
     1021 ccgccgacct gcagcctgaa cggtcccagg ttgagcggta cgccgatgcc tatcacggcg
     1081 ttcatctggc cgtcgaccga gccggtggcg ccgagcaggg tcttgccgct caggtaggcg
     1141 tccgcgccgc cgacgaggcc gaggccgagg cccttgccta cccagccgag gccgaagctg
     1201 gcgccggcgc cgaagccgtt cttggtgacg aggccggtca gggtattgaa catgtcctgc
     1261 tcgtcgccct cgcccgagac gaggccggag accgcgtcga tattctcggt ggtcgggctc
     1321 aggtacagcc agggagtcat cgacagcagg gtcagctcgg ccttcttatc ggcgaaggct
     1381 gcggggttgc cgtagaggct ctggaagccg tcgctcatcg cgacgaaggc gccgcccatc
     1441 gccatgctac gggcgtcctt gggatagaag ttgctcaggg tggtctgggc aaaggccgcg
     1501 cccccgacga ggagggcgat catcgccagt actataacgc gtttcatgtc gtcctcctta
     1561 ctcgccggt
//
LOCUS       DAOOLN010000520         1569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_103082_length_1569_cov_2.777411, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000520 DAOOLN010000000
VERSION     DAOOLN010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103082_length_1569_cov_2.777411"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="PLW80_07165"
     CDS             <1..953
                     /locus_tag="PLW80_07165"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB66322.1"
                     /translation="WLICRAARSGGRKPTEWSLEANPEDVDERLIDTLLEAGVTRLSV
                     GAQSLEEEARRLSARRGTTAETLGRLEVIAARWPGRWSVDLMFGLPGQSAEGLAEDAR
                     AVIGLGAGHVSLYELTLEDGTPLSRAAASGTVSLPDDDERADHYEAARSCLLEAGFRR
                     YEVSNWALDGQECAHNLRYWNLDDWLAIGPSGVGNVASGPGAFRRIANAADLDAYCSD
                     PSGSASESTVDGKEAAFECLMMALRTSRGLSLGSFERRFGMKAMDAFGPLHDRFPGLV
                     RLEGDAWIATDRGLDTLNEPLVDALSSIERSWAGRSPQPV"
     gene            950..>1569
                     /gene="pdxY"
                     /locus_tag="PLW80_07170"
     CDS             950..>1569
                     /gene="pdxY"
                     /locus_tag="PLW80_07170"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HPB66323.1"
                     /translation="MNILSIQSHVAYGHVGNRSAVFPLERLGYEVWPVNTVQYSCNAG
                     IPGWTGAAFGADHVADIITGLERVGAMSRCDAVLSGYVGDAETGRAILEAVSVAKRLN
                     PTTLYCCDPVMGDRPEGLYVRGELPDFFMDEAIPLADIVCPNVFEAELLSGIVIESDD
                     DLARATDAIHAKGPGVVMVTSYRPPSRDRIGLYVSDGRTKLAASAP"
BASE COUNT          267 a          515 c          535 g          252 t
ORIGIN      
        1 tctggcttat ttgtagggcg gcccgctcgg gcgggcggaa gccgacggaa tggagcctcg
       61 aggcgaaccc cgaggacgtc gacgagcggc tgatcgatac cctgctggag gccggcgtga
      121 ccaggctgag cgtcggagcg cagagcctcg aggaagaagc taggcgcctg agcgcccgca
      181 ggggaacgac ggccgagacg ctcggtcggc tcgaggtcat agccgcccgt tggcccgggc
      241 gctggtccgt cgacctcatg ttcgggttac ccggacagag cgccgaggga ctggccgagg
      301 atgcccgggc cgtgatcggg ctaggggccg gacacgtgtc tctgtacgag ctgacgctcg
      361 aggacggcac gccgttgagc cgcgcggctg cgtcgggaac cgtatcgttg cccgacgacg
      421 acgagcgagc cgatcattat gaggccgctc gctcgtgttt gctcgaggcc ggtttcaggc
      481 gctacgaggt gtcgaactgg gcgctcgacg gacaggaatg cgcccataac ctccggtact
      541 ggaacctcga cgactggctg gccatagggc cttccggcgt cggcaacgtc gcgagcggac
      601 ccggagcctt tcgcaggata gccaacgccg cggatctcga cgcctactgc tccgacccat
      661 cgggtagcgc gtctgaatcg accgtggatg gcaaggaggc ggctttcgaa tgcctgatga
      721 tggccctgag aacctctcga ggcctgtccc tgggctcgtt cgaacgacgc ttcgggatga
      781 aagcgatgga cgccttcggt cccctccatg atcgcttccc cggactcgtg cgcctcgagg
      841 gcgacgcgtg gatcgccacc gaccgcgggc tcgatacgct caacgagccg ctcgtcgacg
      901 ccctgtcctc gatcgagcgt tcgtgggccg gacggagccc tcaacccgta tgaacatcct
      961 atcgatacaa tcgcacgtgg cctacggcca cgtcggcaac cgctccgcag tattcccgct
     1021 cgaacgcctc ggctacgagg tctggccggt caacaccgtg cagtactcgt gcaacgccgg
     1081 gattcccgga tggaccggcg cggccttcgg cgcggaccac gtcgccgata tcatcacagg
     1141 cctggaacgc gtgggggcga tgagccgctg cgacgcggtg ctttcaggct acgtcggcga
     1201 cgctgagacg ggacgcgcca tcctcgaggc cgtctccgtc gccaagcggc tgaacccgac
     1261 gaccctctac tgctgcgacc cagtcatggg cgacaggccc gaaggcctct acgtacgcgg
     1321 cgagttgccc gacttcttca tggacgaggc gatcccgctg gcggacatag tctgccccaa
     1381 tgtgttcgag gccgagctgc tgtcgggtat cgtgatagaa tccgacgacg acctggcgcg
     1441 ggccaccgac gcgatccacg ccaaggggcc gggcgtggtg atggtgacca gctataggcc
     1501 gccatcaaga gaccgcatcg gcctgtacgt gagcgacggc cggacgaaac tggccgcgtc
     1561 cgcgcccat
//
LOCUS       DAOOLN010000521         1559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_104068_length_1559_cov_3.394947, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000521 DAOOLN010000000
VERSION     DAOOLN010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104068_length_1559_cov_3.394947"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="PLW80_07175"
     CDS             complement(<1..1110)
                     /locus_tag="PLW80_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HPB66324.1"
                     /translation="MKIRTAFSLFVAVVLIVGVAVSLLMGILPAREYIQKNFYASVGN
                     IVDASKSGIQTEIARGWEVSLALARNPFLADWLLGDERDTAAGRKVLSMAVEYASRTG
                     FSSSFIANRSTGSFYSGDKLMQVLSPTRADDSWFFDVLGSDKELTLNLDYNEQLGSTS
                     LWFNTQVLKGRAVIGVAGIALSIDKVVNDFKAAVPSANSGLHLVDSSGMVVVSSDDEA
                     IGKELRSLISGTAKSIPGLADVKTFDDPVLGPTLLSEEQILESGYSIAFVGPVSDFVP
                     SFISLSGGSILFTAVFAALVVTGAVLFISRRFAAPILEMNRLALGLAAGDLSVSADAS
                     ISRRNDEIGTLAQSMSATMERLSGVVSEVQETANKV"
     gene            complement(1141..>1559)
                     /locus_tag="PLW80_07180"
     CDS             complement(1141..>1559)
                     /locus_tag="PLW80_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66325.1"
                     /translation="GTQQINQAIAQLDTVIQQNAALSEEFSATSEEISGQATTVAGTT
                     EELAAQAARLKEVMSFFRLGSAGASGTAPKAPKPVKISERPGTEKPAVMDTPVKVAPV
                     PKPATRGVTVKKPSTAITLKAQPKRDSRDDDDFIEY"
BASE COUNT          267 a          502 c          494 g          296 t
ORIGIN      
        1 gactttgttg gcggtttcct gcacctcgga gacgacgccg gacagccgct ccatcgtcgc
       61 gctcatcgac tgggccagcg tgccgatctc gtcattgcgc cgcgatatcg acgcgtcggc
      121 gctgacgctc aggtcgccgg cggccagccc gagcgccagc cggttcatct ccagaatcgg
      181 cgccgcgaaa cgcctcgata tgaacagcac ggcgcccgtg acgacgaggg ccgcgaagac
      241 ggccgtgaat agtatcgaac cgccgctcag gctgatgaac gacggcacga agtccgacac
      301 gggaccgacg aaggcgatgg agtagccgga ttccagtatc tgctcctcgg acagcagggt
      361 cggaccgaga accgggtcgt cgaaggtctt gacatcggcc aggccaggga tgctcttcgc
      421 cgtcccgctt attagcgagc gcagctcttt gccgatcgcc tcgtcgtcgg acgagacgac
      481 caccatgccg gacgagtcca ccaggtggag cccgctgttc gcgctcggca cggccgcctt
      541 gaagtcgttg acgaccttgt cgatggacag cgcgatgccg gcgacgccta tgaccgcccg
      601 gccctttagc acctgggtgt tgaaccatag cgacgtcgag ccgagctgct cgttatagtc
      661 gaggttcaag gtcagttcct tatcggagcc caacacgtcg aagaaccagg agtcgtccgc
      721 cctcgtgggc gacaggacct gcatgagctt atctccagag tagaacgatc cggtgctacg
      781 gttcgctatg aagctcgaag agaagccggt cctcgaggcg tactcgacgg ccatcgatag
      841 caccttgcgg ccggcggccg tgtcccgctc gtcgccgagc agccagtcgg cgaggaacgg
      901 attcctggcc aacgccagag acacctccca gccgcgggct atctcggtct gtatgcccga
      961 tttcgaggcg tctacgatat tgcccacgct ggcgtagaaa ttcttctgta tatattcgcg
     1021 cgccggcagg atgcccatca gtagggatac cgccactcca acgatgagga cgacggctac
     1081 gaatagtgaa aaggcggtcc gtatcttcaa atcctgtcct cgcttcctgt atccctcgtg
     1141 ctagtactcg atgaaatcgt cgtcgtcacg ggagtcgcgc ttgggctgcg ccttgagcgt
     1201 gatcgcggtc gacggtttct tgaccgtcac gccccgcgta gccggcttgg ggacgggtgc
     1261 gaccttgacg ggggtatcca tgaccgccgg cttctcggtg cccggtcgct cggagatctt
     1321 gaccggcttg ggcgccttgg gcgcggttcc ggaggcgccg gccgacccga gcctgaagaa
     1381 cgacatgacc tccttgagcc gcgcggcctg ggccgccagc tcctcggtcg tgccggcgac
     1441 agtggtcgcc tggcccgata tctcctcgct cgtcgcgctg aactcctcgg acagcgccgc
     1501 gttctgctgt atgaccgtgt cgagctgggc tatagcctgg ttgatctgct gggtgcccg
//
LOCUS       DAOOLN010000522         1555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_104510_length_1555_cov_2.721333, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000522 DAOOLN010000000
VERSION     DAOOLN010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104510_length_1555_cov_2.721333"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="PLW80_07185"
     CDS             <1..907
                     /locus_tag="PLW80_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB66326.1"
                     /translation="ETLVKAIPLMLTGLGVALAFKLRFWNIGAEGQLTLASIAATWVA
                     LYWNWLPGPLVLPVLVLVGCAAGALWAGVPALLKTRLNVDETLVTLMLNYVALLLAEF
                     LYYGPWRDPKGFGFPGTRLFPKEAWFPRIAGRAHIGLYFAIAVALILWFVLNRTRWGF
                     ELKVIGSSPKAARYQGIAVERNIMLAILLSGAVCGLAGVGEVAGISRRLQQGMALGYG
                     FTAIIVAWMAQLNPLAVPFVALLMGALLVGGDQVQMTMGLPSSIGTLMQGMILFPMLA
                     GSLFTEYRIKRIGAQAVRESETGAA"
     gene            907..>1555
                     /locus_tag="PLW80_07190"
     CDS             907..>1555
                     /locus_tag="PLW80_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB66327.1"
                     /translation="MGLITSLLAITLRAGTSLVYATIGETYTERAGILNLGLEGIMLM
                     GAVSSFATAYYSGSLALAIVVALCVGATLSLIHAFLTVTMKANQVVSGLSITLFGTGF
                     ASFLGQRLGPASNGRYLIGLTGPRFTPIDIGPLKDVPILSAILGQDLFTYGIYILIPL
                     AWFYMYRTRNGLWLRAIGENPRTADAMGVDVNRSKYAYTIFGGMMTSLGGAHLALA"
BASE COUNT          228 a          555 c          486 g          286 t
ORIGIN      
        1 cgagaccctg gtcaaggcga taccgttgat gctgaccggc ctcggcgtgg ctctcgcgtt
       61 caagctacgc ttctggaaca tcggcgccga gggccagctg acgctggcga gcatcgcggc
      121 cacctgggtc gccctatact ggaactggct gcccggcccg ctcgtgctgc cggtgctggt
      181 cctggtcggc tgcgcggccg gagcgctctg ggccggcgtg ccggccctcc tcaagacgcg
      241 gctcaacgtc gacgagaccc tcgtcacgct gatgctcaac tacgtggcgc tgctcctggc
      301 tgaattcctg tactacggcc cctggcgcga cccgaagggt ttcggcttcc cgggcacgag
      361 gctcttcccc aaggaggcct ggttcccgcg catcgccggc cgcgcccaca taggcctgta
      421 cttcgcgata gccgtggcgc tgattctgtg gttcgtgctc aaccgcacgc gctggggctt
      481 cgagctcaag gtgataggct cgagccccaa ggccgcgcgc taccagggca tagccgtcga
      541 gcgcaacatc atgctcgcga tcctgctgtc cggggccgtc tgcggcctgg ccggcgtcgg
      601 cgaggtcgcc ggcatctcga ggcgcctgca gcagggcatg gcgctcggct acggcttcac
      661 cgccatcatc gtcgcctgga tggcccagct caacccgctg gccgtgccct tcgtcgccct
      721 cttgatgggc gcgctcctcg tcgggggcga ccaggtgcag atgacgatgg gcctcccgtc
      781 gtcgataggc accctcatgc agggcatgat cctgttcccg atgctggccg gatcgttgtt
      841 caccgaatac aggatcaagc ggataggcgc ccaagccgtc cgcgagtccg agacaggagc
      901 cgcctgatgg gcctcataac ctccctgcta gccattacgt tgcgcgccgg cacgagcctg
      961 gtctacgcga ccataggcga gacctatacc gagcgcgcgg gcatactcaa cctcggcctc
     1021 gagggcatca tgctgatggg cgccgtctcg agcttcgcca cggcctacta ttccggtagc
     1081 ctcgcgctgg ccatcgtcgt cgccctatgc gtcggcgcca cgctatcgct gatccacgcc
     1141 ttcctgacgg tgacgatgaa ggccaaccag gtggtctccg gcctgtcgat cacgctgttc
     1201 ggcaccggct tcgcgagctt cctcggccag cgcctcgggc cggcctccaa cggccgttac
     1261 ctgatcggcc tgaccggccc gcgcttcacg cccatcgaca tcggcccgct caaggacgtg
     1321 cccatactca gcgcgatcct cgggcaggac ctgttcacct acggcatcta catcctgatc
     1381 cccctcgcgt ggttctacat gtataggacg aggaatggcc tgtggctgcg cgcgatcggc
     1441 gagaatccgc gtaccgccga cgcgatgggc gtggacgtga accgctccaa gtacgcctac
     1501 acgatcttcg gcggcatgat gacgtccctc ggcggcgccc acttggcgct cgcct
//
LOCUS       DAOOLN010000523         1554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_104581_length_1554_cov_4.174783, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000523 DAOOLN010000000
VERSION     DAOOLN010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104581_length_1554_cov_4.174783"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PLW80_07195"
     CDS             <1..780
                     /locus_tag="PLW80_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HPB66328.1"
                     /translation="VRGRLGLDAAPMATRRLSASLMADSDQAALAWLRDYAASLAGDS
                     LSAWGLPRDAALVSGLLDALAASSGRAFNENAAARSLGVSRPTIERAVAAVERAGLLL
                     RVPAWRPRDDIARDDGLPGTARAIRSPAIHLADSGLLHALHGAAPTLETAATAASWTS
                     FVIGQALSVLRPGAAIFHYASADGAALELVVAMDGRPVVAAAARRHRPSSAERAVSYA
                     ARAIGATERYIVVPEGGGAKLPGGFIVTGLRAFLDRLVAVP"
     gene            811..1527
                     /locus_tag="PLW80_07200"
     CDS             811..1527
                     /locus_tag="PLW80_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB66329.1"
                     /translation="MLEVRGFVKRFGSRTVLDHLDAVVDEGRVTALVGPNGSGKSTLL
                     KALWGEAPADSGKLLWRGEPIDARSTAWKRQVGAVPDDDALIEGLSIGGHFALCGGLS
                     GLSPATVEDRSERLVRLFALEDAMATTRMADEASRGNRKRLACALALLGEPELLLMDE
                     PFSGLDAERAAALTSILRALASRGLTILLSCHDDGITRRVADRFLLLRDGKAVTGQIG
                     ELPRGPATVPDTEDLLPWLG"
BASE COUNT          216 a          551 c          553 g          234 t
ORIGIN      
        1 gttcgaggca ggctcggcct cgatgcggcg cctatggcta cgaggcggct ttccgcaagc
       61 ctcatggcgg acagcgacca ggccgcgctc gcctggctac gcgactacgc cgccagccta
      121 gccggagact cgctgtcagc ctggggccta ccgcgcgacg cggccctcgt ctcggggctg
      181 ctcgacgccc tggcagcctc gagcgggcgc gccttcaacg agaacgccgc ggccaggagc
      241 ctcggcgttt caagaccgac gatagaacgc gccgtggcgg ccgtcgagcg ggccgggctc
      301 ctgctacgcg tgccggcctg gcggccgcga gacgatattg cgcgagacga cggtctgccg
      361 ggcacggcgc gtgccatccg atcgccggcg atccacctgg ccgactcggg gctactccat
      421 gcactgcacg gcgccgcccc gacgctcgag actgcggcca cggccgcctc gtggacgtcc
      481 ttcgttatcg ggcaggccct gagcgtcctt aggcctggag cggctatctt ccattacgcc
      541 agcgcggacg gagcggccct cgagctggtc gtggccatgg acggccggcc cgtcgtcgcg
      601 gcagccgcgc gccgccatcg tccgagttcc gcggagcggg ccgtatcgta cgcggcgcgg
      661 gcgatcggcg ccacggagcg ctacatcgtc gtgcccgagg gaggcggcgc gaagttgccc
      721 ggtggcttca tcgtcaccgg cctgcgggcc ttcctggatc gactcgtggc cgtaccttga
      781 ccggcgtgga gagcggacgc acactaagcc atgctcgaag tacgtggatt cgtcaagcgc
      841 ttcggtagcc ggacggtgct cgaccacctc gacgctgtcg tcgacgaggg ccgggtgacg
      901 gcccttgtcg gcccgaacgg ctccggcaag tcgaccctgc tcaaggcgct ctggggcgag
      961 gctccggccg actcgggcaa gctactctgg cgtggcgagc ctatcgacgc tcgctcgacg
     1021 gcttggaagc gccaagtggg cgcggtaccc gacgacgacg cgctgatcga gggcctgtcg
     1081 ataggcggcc acttcgcgct gtgcggcgga ctgtccggac tgagcccggc gaccgtcgag
     1141 gatcggtctg aacggctcgt caggctgttc gcgctcgagg acgccatggc gacgaccaga
     1201 atggccgacg aggcctctcg cggcaaccgt aagcgcctgg cctgcgcgct tgccctgctc
     1261 ggcgagccgg agctactcct catggacgag ccgttctccg ggctcgacgc ggaacgggcc
     1321 gccgcgctga cgtccatcct ccgggccttg gcgtcccgag gattgacgat cctgctatcc
     1381 tgccacgacg acggcatcac gcggagggta gctgaccgct tcctgctcct gcgggacggc
     1441 aaggcagtga ccggccagat aggcgagttg ccgcgcggcc ccgcgactgt cccggacacg
     1501 gaggacctgc tgccgtggct cggctgagcc gcaccaggcc gacggggctc gtcc
//
LOCUS       DAOOLN010000524         1553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_104717_length_1553_cov_2.798398, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000524 DAOOLN010000000
VERSION     DAOOLN010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104717_length_1553_cov_2.798398"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /locus_tag="PLW80_07205"
     CDS             <1..1231
                     /locus_tag="PLW80_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPB66330.1"
                     /translation="VDVVVLDVWLPRMGGIDVLKAIKADHPAVETIVVSGHASIDMAV
                     SAVKLGAFDFIEKPLSIERLITAVRNARALSDLRVENARLKRSAVESDDIIGASDPMA
                     YVRDLIYQSARSDARVLITGENGTGKELVARRIHELSERASGPFVAVNCAAMPDTLIE
                     SELFGHEKGSFTDAVARRKGKFETAHTGTLFLDEIADMSLSAQAKMLRAVQEMRFERL
                     GGESTIEVDVRVIAATNKDLRQEIAAGRFREDLFFRLNVIPLRIPALRERADDIPELT
                     AFFLARFSGGRVRRMSDEAMATLKAHDWPGNVRELKNFIERIAVMSDEDVISGETVRH
                     FMLKSDGSSPEAAPDRLDELLNIRYSEAKEAFERGYLHHNLRKHGYNIVKTAEAIGIY
                     PKNLYAKIKKFGIEAGE"
     gene            1228..>1553
                     /locus_tag="PLW80_07210"
     CDS             1228..>1553
                     /locus_tag="PLW80_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HPB66331.1"
                     /translation="MNDLTDRQKEVLDYIESFIWENPYPPTIREIAAHFSMSVRGAYD
                     HLKALERKGRIRAGGGRSRKIEIIGKPTRQEHNAAVEVPLLGSVAAGKPIFAEDKRFL
                     FYCASG"
BASE COUNT          331 a          479 c          482 g          261 t
ORIGIN      
        1 cgtcgacgtc gtcgtcctgg acgtatggct accgaggatg ggcggcatcg acgtactcaa
       61 ggcgatcaag gccgaccatc cggcggtgga gaccatcgtc gtgtcgggcc atgcgtccat
      121 cgatatggcc gtcagcgccg tcaagctcgg cgccttcgac ttcatcgaga agcccttatc
      181 gatcgagcgg ctgatcaccg cggtcaggaa cgccagagcc ctgtcggatc tgcgcgtgga
      241 gaacgccagg ctgaagcgct cggcggtcga gtcggacgac atcataggcg cctcggaccc
      301 gatggcctac gtacgcgacc tgatctacca gagcgcccgc tcggacgccc gagtgctgat
      361 caccggcgag aacggcaccg gcaaggaact cgtggccagg cgcatccacg agttgtcgga
      421 gcgggcctcg gggcccttcg tagcggtgaa ctgcgccgcc atgcccgaca ccctgatcga
      481 gagcgagctg ttcggccacg agaagggttc cttcaccgac gcggtagcca ggcgcaaggg
      541 caaattcgag acggctcata ccggcacgct attcctcgac gagatagccg acatgtctct
      601 ctcggcccag gccaaaatgc tccgggccgt ccaggagatg cgcttcgagc gcctcggagg
      661 cgagagcacc atcgaggtgg acgtacgggt gatcgccgcc acgaacaagg atctcaggca
      721 ggagatagcg gccggccgct tcagggagga cctgttcttc agactcaacg tcatcccgct
      781 acgtatcccc gccctgaggg agcgggccga cgatataccc gagcttacgg ccttcttcct
      841 cgctcgcttt tcgggtggaa gggtcaggcg catgtccgac gaggccatgg cgacgctcaa
      901 ggcccacgac tggccgggga acgtgcgcga gctcaagaat tttatcgagc gtatagcggt
      961 gatgagcgac gaggacgtca tctcgggcga gaccgtgcgg catttcatgc tcaagagcga
     1021 cggctccagc ccggaagccg ccccggaccg gctcgacgag ctattgaata ttaggtattc
     1081 cgaggcaaaa gaagccttcg aacgaggtta tttacaccac aacttgagaa aacatggcta
     1141 taatatcgtg aagacagccg aagcgatcgg gatttacccg aagaacctct acgccaagat
     1201 caagaaattc ggtatcgagg ccggcgaatg aatgacttga cggatcgcca aaaggaagtg
     1261 ctcgattata tcgagtcctt catctgggaa aacccctacc ccccgaccat acgcgagata
     1321 gccgcccatt tttctatgtc agtccgcggc gcgtacgacc acctgaaggc cctcgagcgc
     1381 aagggccgca tcagagccgg cggcggtcgg tccaggaaga tcgagatcat cggtaagccg
     1441 accaggcagg agcacaacgc cgccgtcgag gtaccgttgc tcggctccgt ggccgcgggc
     1501 aagccgatct tcgccgagga caagcgcttc ctgttctact gcgccagcgg ctg
//
LOCUS       DAOOLN010000525         1553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_104721_length_1553_cov_2.732977, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000525 DAOOLN010000000
VERSION     DAOOLN010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104721_length_1553_cov_2.732977"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PLW80_07215"
     CDS             complement(<1..427)
                     /locus_tag="PLW80_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HPB66332.1"
                     /translation="MKARVRYAPSPTGLQHIGSVRTALFNYLFARSVGGTFILRLEDT
                     DRTRSQDVYVKNLYDTFRWLGFYWDEGPDVGGPVGPYTQSERFALYREHAERLVDMGK
                     AYYCFCSDDKSAAKDAEKSGQDQAEQEGDAQASGGFGYPR"
     gene            540..1076
                     /locus_tag="PLW80_07220"
     CDS             540..1076
                     /locus_tag="PLW80_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66333.1"
                     /translation="MKAIKPAIASVLLALAALSPVLAQDGEGSVFAPYPSRIRIGVRG
                     REIVLSWEDSSDIVSGYAVYRHDEFPDALNFERALLLGYSDSGSKGFAYAPVDDRPYY
                     YFVLGRVPEPVPEGSPAEYRIFIPLRNVPIEPVALAAQAVPAAAPTVAASSERPRPRS
                     LASPASWPGPMATPSSYP"
     gene            complement(1228..1371)
                     /locus_tag="PLW80_07225"
     CDS             complement(1228..1371)
                     /locus_tag="PLW80_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66334.1"
                     /translation="MLLEAAPDDGNPTRNAPALSDTGEAVALAPALSSTMPATRSFSG
                     MIA"
     gene            1370..>1553
                     /locus_tag="PLW80_07230"
     CDS             1370..>1553
                     /locus_tag="PLW80_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66335.1"
                     /translation="MPLPYLVLTRGFEDAKPVSAGDPTPRSRALKAETEKAIAALVST
                     SGSATRAERPPITIFPE"
BASE COUNT          249 a          538 c          496 g          270 t
ORIGIN      
        1 tgcggggata gccgaagcca cccgaggcct gggcgtcgcc ctcctgctcg gcttggtctt
       61 ggccggactt ctcggcgtcc ttggcggccg acttgtcatc ggagcagaag cagtagtagg
      121 ccttgcccat gtcgacgagg cgttcggcgt gctcgcggta cagcgcgaag cgctccgact
      181 gcgtatacgg gccgacgggg ccgccgacgt ccggaccctc gtcccagtag aagccgagcc
      241 agcggaaggt gtcgtacagg ttcttgacgt agacgtcctg ggaccgggtg cgatcggtgt
      301 cctcgagcct gaggatgaag gttccgccga ccgacctggc gaacaggtaa ttgaacaggg
      361 cggtgcgaac gctccctata tgctgcaggc ccgtcggcga cggggcgtat ctgactctgg
      421 ccttcatcgt ggctatcctc cgttgctgag tactcagacc gcgagtatat cggcctccgc
      481 ggggcctgat caatcctgcg ctatggagcg agcttcaatt ttccgatact ataggggata
      541 tgaaagcgat aaaacccgcg atcgcgtcgg tgctgctagc gctggcggct ctgtcccccg
      601 tcctggccca ggacggcgaa ggctcggtgt tcgcgcccta cccgtcacgc atacggatag
      661 gcgtgcgcgg ccgcgagatc gtgctgtcct gggaggacag ctccgatata gtgtcgggct
      721 acgcggtcta ccgccacgac gagttccccg acgcgttgaa tttcgagcgg gccctgctcc
      781 tcggctattc cgatagcggc tccaagggtt tcgcctacgc gccggtcgac gataggccat
      841 actactattt cgtgctcggg cgcgtccccg agccggtccc cgaaggctcg ccggccgagt
      901 accgtatctt catcccgcta cgcaacgtac cgatcgagcc ggtggccctg gcggcccagg
      961 ccgtcccggc ggccgcaccg acagtcgcgg cgagctccga gaggccaagg ccgcgaagcc
     1021 tcgcctctcc ggcatcctgg ccagggccga tggcgacgcc atcgtcgtat ccatagactc
     1081 cgacggggat tcgggcaggc tcgtcgtcta ccgcggcacc tcgccaatac gctcgtcgtc
     1141 ggacctgctc gacgcggcgc tcgccgccat cgtcgaacaa gcctccggcc cctaccgcga
     1201 ctatcccgtc ccgggcgtcg attactacta cgctatcata ccggagaaag acctggtcgc
     1261 cggcatcgtc gagctcaagg ccggcgccaa cgcgacggcc tcgcccgtat cgctcagggc
     1321 cggagcgttc cgcgtcgggt tgccgtcgtc gggcgccgcc tccaggagca tgccgctacc
     1381 ctacctcgtg ctgaccagag gcttcgagga cgccaaaccg gtctcggccg gagacccgac
     1441 tccccggagc cgcgccctca aggctgagac cgagaaggcc atcgccgcgc tcgtctcgac
     1501 gtccgggtca gcgacccggg cagagcgccc accgataacg atcttccccg agg
//
LOCUS       DAOOLN010000526         1552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_104819_length_1552_cov_2.676687, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000526 DAOOLN010000000
VERSION     DAOOLN010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104819_length_1552_cov_2.676687"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PLW80_07235"
     CDS             complement(<1..555)
                     /locus_tag="PLW80_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB66336.1"
                     /translation="MMKLYRLKSAWLCMAALSVCAPALVFSDGAARELVVGMGTYDVE
                     LNPYKSIYAHEMQLFTALYEGLFSYDPQSLDPVRAQAESFEKSADGKTWTFRMRAGAR
                     WADGTPVTAGDFVESWLYLLSPRTKAEYAVFFDIIQGAKDYRTGKSSSPESVGLRAPD
                     DRTLVVELTSPAAYFTRLLCHSAFV"
     gene            complement(586..>1552)
                     /locus_tag="PLW80_07240"
     CDS             complement(586..>1552)
                     /locus_tag="PLW80_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66337.1"
                     /translation="GTDPLSTGIINEGLLQLDKASRKIFQTLKALTLIHKERYKLELR
                     RLVMNGLALDRDYAVTHQDDAVRKIRQKFTEVAGDRPFYAELVAEALKEDYGAEGESL
                     RDGILAALKVAEAKSGDKSAERNYKAALLDGARVLIGVGFQLEDAVRKLEENQALLES
                     MDRSFLTKVKRAIREMLGKKGEHVVHEVEYLDPVTSERKNESIDFTAFCREAAKRAQS
                     LAAMVSKSSAAFKRMEGSSEDVIYKFLSKSIEELQAFHRKLSALDEFFQGAIEDPEFK
                     GRVRSVKIELGAIKNAIIKSNQKRHEYIAQKEELEQMKRLGIREA"
BASE COUNT          256 a          497 c          472 g          327 t
ORIGIN      
        1 cacgaaggcg ctatggcaca ggagccgcgt aaaataagcc gccggcgacg tgagctcgac
       61 gaccagggtc ctatcgtcgg gcgcgcgcag cccgactgac tcgggcgagc tactcttgcc
      121 ggtccgatag tccttggcgc cctgtattat atcgaagaac acggcgtact cggccttggt
      181 ccttggcgac aggagataga gccacgactc gacgaagtcg ccggccgtga ccggcgtgcc
      241 gtcggcccag cgggcgccag cccgcattct gaaggtccag gtcttgccat cggcggactt
      301 ctcgaaggac tccgcctggg cgcgtaccgg gtccagactc tgcggatcgt agctgaacag
      361 cccctcgtat agcgcggtaa acagctgcat ttcgtgggcg taaatggact tatacgggtt
      421 cagctcgacg tcgtaggttc ccatgcccac gacgagctcc cgggcggcgc catcgctgaa
      481 gacgagcgcc ggcgcgcata cggacagcgc agccatgcat agccaggcgc tcttgagtcg
      541 atacagcttc atcatcgctc ctcggtgggt cggcggaacg cgcgcctagg cttcccggat
      601 gcccaagcgt ttcatctgct cgagctcttc cttctgcgct atgtactcat gccgcttctg
      661 gttcgatttg attatagcgt tcttgatggc gcccaactcg attttgacgc tacggacgcg
      721 ccccttgaac tcgggatcct cgatcgcgcc ttggaagaac tcgtcgaggg ctgatagctt
      781 gcggtggaag gcctgtagct cctcgatgct cttggacagg aacttgtaga tgacgtcctc
      841 cgaggagcct tccatgcgct tgaacgcggc gctcgacttg gagaccatcg ccgccaggct
      901 ctgcgcccgc ttggccgcct cgcggcagaa tgccgtgaag tctatcgact cgttcttgcg
      961 ctcgctggtc accggatcga ggtactcgac ttcatggacc acgtgttcgc ccttcttgcc
     1021 gagcatctcg cggatggctc gcttgacctt ggtcaggaac gagcggtcca tcgattcgag
     1081 cagggcctgg ttctcctcga gcttgcgcac ggcgtcctcg agctgaaagc cgacgccgat
     1141 cagcacccgg gcgccgtcga gcagggcggc cttgtagttg cgctcggctg acttgtcccc
     1201 gctcttggcc tcggctactt tgagagccgc cagaatcccg tcgcgcagcg actcgccctc
     1261 ggcaccgtag tcttccttga gcgcctcggc gaccagctcc gcgtagaagg gccggtcgcc
     1321 ggcaacttca gtgaatttct gcctgatttt cctcacggcg tcgtcctggt gggtcaccgc
     1381 gtaatcacgg tcgagggcca ggccgttcat cacgagccgc ctgagttcca gcttgtagcg
     1441 ctccttatgg atcaacgtca gagccttgag ggtctggaat atcttacggc tcgccttgtc
     1501 gagctgcagg agcccctcgt tgatgatacc ggtcgacagc gggtcggtac cc
//
LOCUS       DAOOLN010000527         1552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_104824_length_1552_cov_2.454242, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000527 DAOOLN010000000
VERSION     DAOOLN010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104824_length_1552_cov_2.454242"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /locus_tag="PLW80_07245"
     CDS             <1..1443
                     /locus_tag="PLW80_07245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB66338.1"
                     /translation="DAARFAVASSYWLPVEYGLAVRGGDDFLLYSLNQGVRAIKESGV
                     YSAIYERWFDDSVAAARRRLRNAMMALVAVLAAFLLGGGAAFWWNRQLASNVRARTAE
                     LRDANDELGRLVGEAEDKKEFIAQILESSPRGLVTCDAGGLVSVCNARARVIGLLDPD
                     PVSRPALEYPFLAHFLNPELTARVLGGESMPFSVSEWARPDGARLQIRHATYPQIDHE
                     GRVVGLIFSFEDHTVEQAMRERLAEREKGEALGRIVAGVAHEIRNPLTSIKAFVELLP
                     RKMDDPRFLREMGSFVPREVERVDKLIRDLIDFSRPRRPVRSTVDLGELAESTLALSS
                     PSLDKRGVTTASSAERGLCCSVDADQLRQCVMNFVLNAADSVEEARARGAGTAAAEDR
                     GPDIELAAYASGGKAVVELRDQGAGIAEADLERVFEPFYTTKRGGVGLGLPLSKQYVE
                     ENGGRLELRIGPGGGATVVMEFPLEGRGRG"
BASE COUNT          232 a          527 c          548 g          245 t
ORIGIN      
        1 gacgccgcgc gcttcgccgt ggcctcatcg tactggctgc cggtggagta cggccttgcc
       61 gtccgcggcg gcgacgactt cctgctctac agcttgaacc agggcgtccg cgcgatcaag
      121 gagtccggcg tctatagcgc catctacgag cggtggttcg acgactcggt ggcggccgcg
      181 cggcgccgcc tgcggaacgc gatgatggcc ctcgtcgccg ttctggccgc ctttctgctc
      241 ggcggaggcg cggcgttctg gtggaaccgg cagctggcct cgaacgtgcg cgcgcggacc
      301 gccgagctgc gcgatgccaa cgacgagctc ggccgtctcg tcggcgaggc cgaggacaag
      361 aaggagttca tcgcgcagat cctggaatcc agccctcgcg gcctggtcac ctgcgacgcc
      421 ggcggcctcg tgtccgtctg caacgcccgg gcccgagtca tcggcctgct cgaccccgac
      481 cccgtcagcc gcccggccct cgagtatccc ttcctggcgc atttcctgaa tcccgagctg
      541 acggcgcggg tactcggcgg cgagtccatg ccgttcagcg tctccgagtg ggccaggccc
      601 gacggcgccc gcctgcagat acgccacgcc acctatcccc agatcgacca cgagggccgc
      661 gtcgtcggcc tgatcttctc gttcgaggac catacggtcg agcaggcgat gcgcgagcgt
      721 ctagcggagc gcgagaaggg cgaggccctc ggccgcatcg tcgccggcgt ggcccacgag
      781 atccggaacc cgctcacctc gatcaaggcc ttcgtcgagc tgttgcctcg caagatggac
      841 gacccgcgct tcctgcggga gatgggttct ttcgtgccgc gcgaggtgga gcgggtggac
      901 aagctcatcc gcgatcttat agatttctcg cggccgaggc ggccggtcag gtcgaccgtc
      961 gacctgggcg aactcgccga gtcgacgctg gccctgtcgt cgccctccct ggacaaacgc
     1021 ggcgtcacga cggcctcgag cgccgagcgg ggcctctgct gttccgtcga cgccgaccag
     1081 ctacgccagt gcgtgatgaa tttcgtgctg aacgcggccg actcggtcga ggaggctcgg
     1141 gctcgcgggg ccggaacggc cgcggccgag gaccgcggcc cggatataga gctggcggcc
     1201 tacgcctcag gcggcaaggc cgtcgtcgag ctgcgcgacc agggcgcagg catcgccgag
     1261 gccgacctgg agcgcgtctt cgaaccgttc tatacgacga aacgcggcgg ggtgggtctc
     1321 ggcctgcccc tgtccaagca gtacgtcgag gagaacggcg gacggctcga gctgcggata
     1381 gggccgggcg gcggcgcgac ggtcgtcatg gagtttccgc tggaaggacg agggcgtgga
     1441 taggatactg atcatcgacg acgaggcggc catctgctca tcgctgacct tcgcgctcga
     1501 ggacgcgtac tcggtagaga gcgccgtcga cgcagaagag ggactggcca tg
//
LOCUS       DAOOLN010000528         1552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_104836_length_1552_cov_2.198397, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000528 DAOOLN010000000
VERSION     DAOOLN010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104836_length_1552_cov_2.198397"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PLW80_07250"
     CDS             <1..603
                     /locus_tag="PLW80_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66339.1"
                     /translation="RLASGYEASRTRISIEALDASGAVVSYERGETRLQRAGGRDAVV
                     VVYAEKDGEDASDDWRKRYAKAAEGPARGGDRRDAGGPPPGFDAAPFNPAYAGALSVG
                     KAMARGSAVEVAFSIDTEAGRVEGVVSFAATGAALFSTQSWLEPPPLVSFMRSSTRYA
                     YHRGALVVASMELEGEMSILFVKRRFRMRFEFSEWIPAGG"
     gene            complement(600..>1552)
                     /gene="holA"
                     /locus_tag="PLW80_07255"
     CDS             complement(600..>1552)
                     /gene="holA"
                     /locus_tag="PLW80_07255"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPB66340.1"
                     /translation="GRRNDFIKQLRSRCASEWNGEPEDHRLYAHETPVPALLDLLMNG
                     SLFSPGKFVQYLGADQVKAKADVQAIVEYAKRPADSTVLVLVADGFGVDKAIEAAIPK
                     DAKKVFWELSAQETGRWVREYFSGQGLSVDDDAVEAILELVENNTEALKVECSRLALF
                     FPRGSSVGEDDVERYIAHNRAEDAFSLFDRMATGDFEQALETLAAIQAARDGSGVGLL
                     AGLLWSFRRLSSLHAAMADGQGFEQAARSLRITRRSALASYDAARQRWPRGRCDRLIA
                     FGVDVDARLRAMGQANERTLLELFVYACVVRRSPVPLGRL"
BASE COUNT          226 a          529 c          557 g          240 t
ORIGIN      
        1 cgcctcgctt cgggctacga ggctagtagg acgcgcatct cgatagaggc gctcgacgcc
       61 tcgggggccg tggtctcgta cgagcgcggc gagacgaggc tccagcgggc gggcgggcgc
      121 gatgccgtgg tcgtcgtcta cgccgagaag gacggcgagg acgcgagcga cgactggcgg
      181 aagcgctacg cgaaggcggc ggagggcccc gcgcgcggcg gcgatcgccg cgacgccggc
      241 gggccgcccc cgggattcga cgccgcgccg ttcaacccgg cctacgccgg cgcgctgagc
      301 gtcgggaagg cgatggcgcg gggctccgcc gtcgaggtgg cgttctcgat cgacacagag
      361 gccggccgcg tcgaaggcgt cgtctcgttc gccgctaccg gagcggccct gttttccacc
      421 cagtcctggc tcgagccgcc gcccctggta tcgttcatgc gctcgtcgac gcgctacgcc
      481 taccatcggg gcgccttggt cgtcgcgtcg atggagctgg agggggagat gtccatcctg
      541 ttcgtcaaaa ggcgtttcag gatgcgcttc gagttctctg agtggattcc ggccggaggt
      601 tagagccgtc cgagcggtac cggggaccta cgcaccacgc aggcgtacac gaacagctcg
      661 aggagcgtcc gctcgttggc ctggcccatc gcccgcagcc tggcgtccac gtccaccccg
      721 aaggctatca gccggtcgca gcggccgcgc ggccagcgct ggcgcgccgc gtcgtagctg
      781 gccagcgcgg atcggcgggt gatgcgtagc gagcgcgccg cctgctcgaa gccctgaccg
      841 tcggccatcg cggcgtggag cgacgagagg cgtctgaacg accaaaggag ccccgctagc
      901 aggccgaccc ccgagccgtc ccgtgcggcc tggatcgccg cgagggtctc gagcgcctgc
      961 tcgaagtcgc ccgtggccat cctgtcgaac aggctgaacg cgtcctcagc ccgattatgg
     1021 gcgatgtagc gctcgacatc gtcctctccg acgctcgagc cgcgcgggaa gaacagcgcc
     1081 aggcgcgaac actcgacctt gagggcctcg gtgttgttct cgaccagctc gaggatggcc
     1141 tcgacggcgt cgtcgtcgac ggacaggccc tggcccgaga agtattccct gacccagcgg
     1201 ccggtctcct gcgcggacag ctcccagaac accttcttgg cgtccttcgg tatggcggcc
     1261 tcgatggcct tatccacccc gaagccgtcg gcgacgagca cgagcacggt cgagtcggcc
     1321 gggcgcttgg cgtactcgac gatggcctgg acgtcggcct tggccttgac ctggtcggcg
     1381 ccgaggtact gcacgaattt gcccggcgag aacagcgagc cgttcatcag gaggtcgagc
     1441 agcgccggca ccggggtctc gtgggcgtag agcctatggt cctccggctc gccgttccat
     1501 tcggaggcgc agcgcgagcg cagctgcttg atgaagtcgt tgcggcggcc ca
//
LOCUS       DAOOLN010000529         1550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_105017_length_1550_cov_2.531104, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000529 DAOOLN010000000
VERSION     DAOOLN010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105017_length_1550_cov_2.531104"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="PLW80_07260"
     CDS             <1..1003
                     /locus_tag="PLW80_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB66341.1"
                     /translation="DEREQTLNQMLVEMDGFDTKDGVIILAATNRPDVLDPALLRPGR
                     FDRQVVVAMPDIQEREDILKIHMAKVPMAEGVDAQRLSRATPGMSGADLANMVNEATL
                     FAVRRGRNVVEMQDFEEARDKIMMGIARTSMVISEEEKKATAYHEAGHALLHYYLPNA
                     DPLHKVTIVPRGRALGVAFSLPAQDAYSRARGWIRDRIVISYGGYAAEARGYGETTTG
                     TAQDLKQATDLARKMACEWGMSEELGAVAYGQEDEPIFIGKEIAQHKDYSEDTAMRID
                     RAVFNILEEAKKKAFDILTEHRDQLLSLAEELIRKETLLDDDIKAMFGFKSPEANEA"
     gene            1053..>1550
                     /locus_tag="PLW80_07265"
     CDS             1053..>1550
                     /locus_tag="PLW80_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66342.1"
                     /translation="MLISTLPAAADSTARDAAICASLTQGAVAALALGHTDQALALLD
                     EALSYRPTDPDACYLSALARLSRGDGVGPAAGLLEVALSVGGFRLYDEGGARLLYAGI
                     LERTGRPAEALRFLSGLPPSAESLFIGAKAALAMGDEAEARAAVLSSLRRYPFDPRPL
                     LAWLRY"
BASE COUNT          278 a          525 c          519 g          228 t
ORIGIN      
        1 cgacgagcgc gagcagacgc tcaaccagat gctggtcgag atggacggct tcgacacgaa
       61 ggacggcgtc atcatcctcg cggcgacgaa ccgcccggac gtcctcgacc cggccctgct
      121 caggcccggc cgcttcgacc gccaggtcgt cgtcgccatg cctgacatcc aggagcgcga
      181 ggatatcctc aagatacaca tggccaaggt gccgatggcc gagggcgtcg acgcccagcg
      241 gctttcacgc gccaccccgg gcatgagcgg cgccgacctg gccaatatgg ttaacgaggc
      301 gacgctattc gccgtcaggc ggggtcggaa cgtcgtcgag atgcaggact tcgaggaagc
      361 ccgcgacaag atcatgatgg gcatagcccg cacctcgatg gtcatctccg aggaggagaa
      421 gaaagcgacg gcctaccatg aggccggcca cgcgctgctc cactattacc tgccgaacgc
      481 cgatccgctc cataaggtga ccatcgtccc acgggggcgc gccctcggcg tggccttctc
      541 gctaccggcc caggacgctt actcgcgcgc tcgcggctgg atcagggata ggattgtcat
      601 ctcgtacggc ggctacgcgg ccgaagctag agggtacggc gagacgacga ccggcacggc
      661 ccaggacctg aagcaggcga ccgacctggc caggaagatg gcctgcgaat ggggcatgtc
      721 cgaggagctc ggagccgtcg cgtacgggca ggaggacgaa ccgatcttca taggcaagga
      781 gatagcccag cataaggact attcggagga tacggcgatg cgcatcgacc gcgcggtgtt
      841 caatatcctc gaggaagcca agaagaaggc gttcgacatc ctgaccgagc accgtgacca
      901 gctgctcagc ctggccgagg agctgatccg caaggagacc ctgctcgatg acgacatcaa
      961 ggccatgttc ggtttcaagt ccccggaggc caacgaggcc taggccggga cgcggcgggg
     1021 cagccatctc gctagccctc gccgcgtcgc tattgctcat ctcgacgctg cccgcggcgg
     1081 ctgactcgac cgccagggac gccgccatct gcgcctcgct gacccagggc gccgtcgcgg
     1141 ccctggcgct cgggcatacc gaccaggccc tggccctgct ggacgaggcc ctgagctacc
     1201 ggccgaccga cccggacgcc tgttacctgt cggccctcgc tcgactgtcg cgaggcgacg
     1261 gagtcggtcc ggcggccggg ttgctcgagg tcgcgctatc cgtcggcggc ttcaggctgt
     1321 acgacgaggg cggcgcgagg ctgctgtacg cggggatact cgagcgaacc ggacggccgg
     1381 ccgaggcgct acgcttcctg tcgggactgc ccccgagcgc cgagtcgctg ttcattggag
     1441 ccaaggcggc cctggccatg ggcgacgagg ccgaggcccg cgcggccgtg ctatcgtcgc
     1501 tacgaaggta tcccttcgac ccgcgccccc ttctcgcctg gctacgatac
//
LOCUS       DAOOLN010000530         1549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_105138_length_1549_cov_2.445114, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000530 DAOOLN010000000
VERSION     DAOOLN010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105138_length_1549_cov_2.445114"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..593)
                     /locus_tag="PLW80_07270"
     CDS             complement(51..593)
                     /locus_tag="PLW80_07270"
                     /inference="COORDINATES: protein motif:HMM:NF028185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 N-terminal
                     domain-containing protein"
                     /protein_id="HPB66343.1"
                     /translation="MPEVIHLNTDRTSYIIDALPTGHLEHLYYGPRLRRMADYEPLRQ
                     KNDVPYGTMVNVSQEHKNLGLDDQCLEYSSLGKGDYREPAIELVFADGSRTTDFRVAS
                     TRSYRGRPAIDGLPSATGGEDDVETTEVELRDERGLTLTLHYCVYAAYDVITRFATLR
                     REQGLAAALAWRTEQFARYE"
     gene            complement(586..>1549)
                     /locus_tag="PLW80_07275"
     CDS             complement(586..>1549)
                     /locus_tag="PLW80_07275"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB66344.1"
                     /translation="GERNTISVIPRVFAAVGNIAVGSLGLALVARLGGVAPDVMPTPE
                     QQSRGFFWLAVLIGAAFVVTNVVTVLFVKEKVVVPSDRKFKFRDIFTTIAGNDQLLVI
                     LVAMTLYTLAINLTASLGLYWFKYDVGDEGLYGTFYLTAAAQVLAMVVFPAFAKWFSR
                     RKVFILSAMLPIVGYVSLYVIGKTSGDGIALMAVAGVVLFFGFGFSLVLTSVMLADAV
                     DYGEWKLGVRSESIVFSMQTFMVKFAMALSGLVTGLGLAAFGFVANAAQSAGAVTGIR
                     LLMFAVPMALIVVSLVVYLRGYRLNGVYFEANKAELAERRTHHA"
BASE COUNT          283 a          489 c          510 g          267 t
ORIGIN      
        1 acttcggacg cacgacactg gcagctgaag tgccgctcga gcccggccgc tcactcgtac
       61 cgcgcgaact gctccgtccg ccaggcgagc gccgcggcca ggccctgctc gcggcgcagc
      121 gtcgcgaagc gggtgatgac gtcgtaggcc gcgtagacgc agtaatggag cgtcagtgtc
      181 aggccgcgct cgtcgcgcag ctcgacctcg gtggtctcga cgtcgtcctc gccgccggtc
      241 gcgctcggga gcccgtcgat ggccggcctg ccgcggtagc tccgcgtcga ggcgacgcgg
      301 aagtcggtcg ttcggctacc gtccgcgaat accagctcga tagccggctc gcggtagtcg
      361 cctttaccca agcttgaata ttcaaggcat tggtcgtcga ggcccaggtt cttgtgctcc
      421 tggctgacgt tgaccatggt gccgtagggc acgtcgttct tctgcctgag cggctcgtag
      481 tcggccatgc gcctgaggcg cggtccatag tacaggtgct cgagatgtcc ggtcgggagc
      541 gcgtcgatga tgtagctggt cctatcggta ttcaggtgta tgacctcagg catggtgtgt
      601 tctcctctcc gcgagttcgg ccttattggc ctcgaaatat acgccgttga gccggtagcc
      661 ccgtaggtag acgacgagcg atacgacgat gagcgccatg ggtacggcga acatcaggag
      721 gcgaatgccg gtcacggcgc cggcgctctg ggcggcgttg gcgacgaagc cgaaggcggc
      781 gagccccagg ccggtcacga ggccagagag cgccatcgcg aacttgacca tgaaggtctg
      841 catggagaac acgatgctct cgctacgtac gcccagcttc cactcgccgt agtcgaccgc
      901 gtctgcgagc atcacgctgg tcagcacgag ggagaagccg aagccgaaga acagcacgac
      961 gccagcgacg gccatcagcg ctatgccgtc gcccgaggtc ttgccgatca cgtagagcga
     1021 cacgtagccg acgatgggca acatggccga caggatgaac accttgcgcc gagaaaacca
     1081 tttggcgaag gccgggaaca cgaccatcgc cagcacctgc gcggcggccg tcagatagaa
     1141 ggtgccgtac agtccctcgt cgcccacgtc gtacttgaac cagtacaggc caaggctcgc
     1201 ggtcaggttg atggccaggg tgtacagggt catggcgacg aggatgacga ggagctggtc
     1261 gtttccggct atcgtcgtga atatgtccct gaacttgaat ttccggtcgc tcggcacgac
     1321 caccttttcc ttgacgaaca gcacggtgac gacgttcgtt acgacgaagg ccgcgccgat
     1381 cagcacggcg agccagaaga agccgcgcga ctgctgctcc ggcgtcggca tgacgtccgg
     1441 ggccacgccg ccgagcctcg caacgagggc taggccgagc gaacctacgg ctatgtttcc
     1501 gaccgccgcg aacacgcgcg gtatgaccga tatcgtgttg cgctcgccc
//
LOCUS       DAOOLN010000531         1544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_105606_length_1544_cov_3.274009, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000531 DAOOLN010000000
VERSION     DAOOLN010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105606_length_1544_cov_3.274009"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..489
                     /gene="ybeY"
                     /locus_tag="PLW80_07280"
     CDS             67..489
                     /gene="ybeY"
                     /locus_tag="PLW80_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPB66345.1"
                     /translation="MEEPAWLERAAAFAVRAMDGLGLEDWDLSLYFCGEAFMAGLNAE
                     YRGKDGPTDVLSFSLGEWRDGENGRRYIAGDVVICLGVMERNAAEYGATRDEELKRLI
                     AHGVLHLSGMDHGTNESDEPMLARQEALLSTLAEETIF"
     gene            513..1277
                     /locus_tag="PLW80_07285"
     CDS             513..1277
                     /locus_tag="PLW80_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPB66346.1"
                     /translation="MRRAFRSRPDGDEADEVSSERQDMIRGIQELSETTVKEVMVPRI
                     DTVFIAIDAPRDELLETVTTCGHSRIPVYNETIDDVVGVLYVKDLLLAIAKGEEIDLA
                     AMIRKPFFVPESKRIDSLLREFKRRHVHIAIAIDEYGGVSGIVCMEDIIEEIVGDIQD
                     EFDNEREDIVAIGDAAWLCDARVNLEDLNEETGLGLPVEGFDTLGGFVFDLFGKIPAR
                     YEKVSWRTFDFVVQEMDGHKIGAVKILRRPGDVEDA"
     gene            1274..>1544
                     /locus_tag="PLW80_07290"
     CDS             1274..>1544
                     /locus_tag="PLW80_07290"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB66347.1"
                     /translation="MTRPARRFGTAVALAAFALAAVGAFAQESGAVSARAQYEAGVEL
                     LASGATYRAVDAFMAAVAANPAYADAWAMLARCQYELGEYERAVSF"
BASE COUNT          275 a          481 c          534 g          254 t
ORIGIN      
        1 ctcgaggata gagtatccga agaccaagga gcaggggtga acgccgtagc cgtcgactat
       61 cgcgacatgg aggagccggc ctggctcgag cgcgccgcgg ccttcgccgt ccgcgcgatg
      121 gacgggctcg ggctcgagga ctgggacctg tcgctctatt tctgcggcga ggccttcatg
      181 gccggcctga acgccgagta ccgcggcaag gacggcccga ccgacgtgct gtcgttctcg
      241 ctcggcgagt ggcgcgacgg cgaaaacggc cgtcgctata tcgccggaga tgtggtaata
      301 tgtctgggcg tcatggagcg taacgccgcc gagtacggcg ccacgcgcga cgaggagctt
      361 aagcgcctca tcgcgcacgg tgtcctgcac ctgtccggca tggaccacgg cacgaacgag
      421 tccgacgagc cgatgctcgc caggcaggag gcgctcctat cgacgttagc ggaggaaacg
      481 atattttgaa gacgagcggt ttcttgagcg gcttgaggag ggccttccgc tccaggcccg
      541 acggcgacga ggccgacgag gtgagttcgg agcgccagga catgatacgc ggcatccagg
      601 agctgtccga gaccacggtc aaggaagtga tggtgcccag gatcgacacg gtgttcatcg
      661 ccatcgacgc gccgcgcgac gagcttctcg agacggtgac cacctgcggg cactcgagga
      721 tacccgtcta caacgagacc atcgacgacg tcgtcggggt gctgtacgtc aaggacctgc
      781 tgctcgccat tgccaagggc gaggagatcg acctggccgc gatgatacgc aagcccttct
      841 tcgtgcccga gtcgaagcgc atcgactccc tgctccggga attcaagcgc cggcacgtgc
      901 atatagccat agccatcgac gagtatggcg gcgtgtcggg catcgtctgc atggaggata
      961 tcatcgagga gatcgtcggc gatatccagg acgagttcga taacgagcgg gaggatatcg
     1021 tcgccatcgg cgacgcggcc tggctgtgcg acgcgcgcgt caatctcgag gatctgaacg
     1081 aggagaccgg gctcggcctg cccgtcgagg gtttcgatac gctcggcggc ttcgtcttcg
     1141 acctgttcgg caagattccc gcccgctacg agaaggtgtc ctggcgcacg ttcgacttcg
     1201 tcgtccagga gatggacggc cataagatcg gcgccgtcaa gatactcagg cggccgggcg
     1261 acgtagagga cgcatgacgc ggccggcgcg gcgcttcgga accgcggtgg cgctagccgc
     1321 attcgctctg gcagccgtcg gcgcgttcgc ccaggagtcc ggggccgtct cggcacgcgc
     1381 ccagtacgag gccggcgtgg agttgctggc gtccggcgct acctaccgcg ccgtcgacgc
     1441 gttcatggcg gccgtcgccg ccaaccccgc ctacgccgac gcgtgggcca tgctggcccg
     1501 ttgccaatac gagctgggcg agtacgagcg cgcggtatcc ttca
//
LOCUS       DAOOLN010000532         1544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_105629_length_1544_cov_2.749496, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000532 DAOOLN010000000
VERSION     DAOOLN010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105629_length_1544_cov_2.749496"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PLW80_07295"
     CDS             complement(<1..393)
                     /locus_tag="PLW80_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPB66348.1"
                     /translation="MVNAKINGIPVQVAEGTTILEAARKVNVRIPTLCYNDDLPPWAA
                     CGICIVKVEKNPKMLRACCTPIEEGMSVITHDPEIVQVRKTVIELILSTHPDDCLQCP
                     RNGSCELQTLAAEFGVREQPFEKRLRDLP"
     gene            complement(406..>1544)
                     /locus_tag="PLW80_07300"
     CDS             complement(406..>1544)
                     /locus_tag="PLW80_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPB66349.1"
                     /translation="MTICGYTVGSTKGFVYIRAEYPLAIERLKIALKQAREYGLLGDN
                     ILGTGFSFDIEIRLGAGAFVCGEETALLQSLEGNRGMPIPRPPFPAVKGLWGKPTIIN
                     NVETWANIPVIITKGAEWFAKIGTETSKGTKVFALTGKVNNSGLVEVPMGTTLREIIF
                     DIGGGIKGGKKFKGAQTGGPSGGIITAEFLDTPIDYESLTSLGSMMGSGGLIVMDEDD
                     CMVDVSRFYMEFCVDESCGKCSPCRIGTNQMHALLVKITKGQGTLDDLDKLEKIGLAM
                     KKAALCGLGQTAPNPTLASIKRFRDEYVEHIVDRKCRAGKCKDLVVYSIDKSKCIGCG
                     LCARKCPVNCITGDKTQKFTIDASKCIKCGECFKACKFSAVVKK"
BASE COUNT          238 a          470 c          514 g          322 t
ORIGIN      
        1 cggcaggtcg cgcaggcgct tctcgaaggg ctgctcgcgg acgccgaact cggcggccag
       61 ggtctgcagc tcgcaggagc cattgcgcgg acactggagg cagtcgtccg ggtgggtcga
      121 caggatcagc tcgatcaccg tcttgcgcac ctggactatc tccgggtcgt gggtgatgac
      181 gctcatgccc tcctcgatcg gggtgcagca cgcgcggagc atcttcgggt tcttctcgac
      241 cttgacgatg cagataccgc aggccgccca cgggggaaga tcgtcgttat agcagagcgt
      301 ggggatgcgt acgttgactt tcctggccgc ctcgaggatc gtggtgccct cggcgacctg
      361 taccggtatt ccgtttatct tggcgttgac catggggcct actccttatt tcttcacgac
      421 ggcgctgaac ttgcaggcct tgaagcactc gccgcacttg atgcacttcg atgcgtcgat
      481 ggtgaatttc tgcgtcttgt cgccggtgat gcagttgacc gggcacttgc gggcgcagag
      541 cccgcaaccg atgcacttgc tcttgtctat gctgtagacg acgagatcct tgcacttgcc
      601 ggcgcggcac ttccggtcga cgatatgctc gacatactcg tccctgaagc gcttgatgct
      661 cgcgagcgtc gggttcggcg cggtctggcc gagcccgcag agggcggcct tcttcatcgc
      721 caggccgatc ttctcgagct tgtcgaggtc atcgagcgtg ccctggccct tggtgatctt
      781 gacgagtagg gcgtgcatct ggttcgtgcc gatgcggcag ggcgagcact taccgcagga
      841 ctcgtcgacg cagaactcca tgtagaacct ggacacgtcg accatgcagt cgtcctcgtc
      901 catgacgatg agtccgccgg agcccatcat cgagccgagg gaggtcaggc tctcgtagtc
      961 gatcggggtg tcgaggaatt cagcggtgat gatgccgccg gacggtccgc cggtctgggc
     1021 gcccttgaac ttcttgccgc ccttgatgcc gccgccgatg tcgaagatga tctcgcggag
     1081 cgtcgtgccc atcgggacct cgacgaggcc ggagttgttc accttgcccg tgagcgcgaa
     1141 caccttggtg cccttgctgg tctcggtgcc gatcttggcg aaccattcgg cgcctttggt
     1201 gatgatgacc gggatgttgg cccaggtctc gacgttgttg atgatcgtcg gcttgcccca
     1261 gaggcccttg accgccggga acggcgggcg cgggatcggc atgccgcggt taccctcgag
     1321 cgactgcagc agggccgtct cctcaccgca gacgaaggcg cccgcgccga ggcggatctc
     1381 gatatcgaac gagaagccgg tgccgaggat gttgtccccg aggaggccgt actcgcgggc
     1441 ctgcttcagc gctatcttga gccgctcgat ggcgagcggg tactcggcgc ggatgtagac
     1501 gaagcccttg gtcgagccga cggtgtagcc gcagatcgtc atcg
//
LOCUS       DAOOLN010000533         1543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_105722_length_1543_cov_3.293683, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000533 DAOOLN010000000
VERSION     DAOOLN010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105722_length_1543_cov_3.293683"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="PLW80_07305"
     CDS             complement(<1..919)
                     /locus_tag="PLW80_07305"
                     /inference="COORDINATES: protein motif:HMM:NF040068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB66350.1"
                     /translation="MSRVDPCTLFKTELPKIAKPARYLGGESGSIAKPDAPFRMVLCF
                     PDLYEIGMANNAMRILYAGLNGLDGVACERVFAVAADYERLLRESRTPLYGLETGTPV
                     SSFDILGFTVGYELAATNILSVLDLSGVPLRSADRTDEHPIVIAGGPAITNPTPYSAI
                     LDAVWIGEAEGAFFSLVAELAELKRNGAGRGELLARLRDERSFWMPAAFGTPPKKAIR
                     AIYDGFTEAPYRYAFPMPVVKPVQDHGVVEIMRGCPNGCRFCHAGYFYRPRRLRSPER
                     ILEEVEAQVLEAGHREISLSSLSSGDYPGI"
     gene            complement(916..>1543)
                     /locus_tag="PLW80_07310"
     CDS             complement(916..>1543)
                     /locus_tag="PLW80_07310"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPB66351.1"
                     /translation="MLVLVAALSLLGFRFFKKRYYYWLAYGSAIGSLGLAFSLAGQRL
                     LKDYQVKRLIVFLDPSIDPLGSGWNILQSITAIGSGGVAGKGFLQGTQSHYRYLPQQS
                     TDFIFSIISEELGFIGGIVIFGLFFYILVRLALSMRKLRDVASSSFVAGVFGIIFFHF
                     MINAGMAMGVMPITGIPLLFLSYGGSSLWSICIGVGLCLGVGARRYDS"
BASE COUNT          321 a          463 c          506 g          253 t
ORIGIN      
        1 cgatgcccgg gtagtcgccc gacgatagcg acgacaggct gatctcgcgg tgaccggcct
       61 cgagcacctg ggcttcgacc tcctcgagga tacgttccgg agagcggagc ctgcgggggc
      121 gatagaagta gccggcgtgg cagaagcggc agccgttcgg gcagccgcgc atgatctcga
      181 cgacgccgtg gtcctggacc ggtttgacga cgggcatcgg aaaggcataa cgatagggag
      241 cctcggtgaa accgtcgtag atcgctcgga tggctttctt agggggcgtg ccgaacgccg
      301 ctggcatcca aaacgatctt tcatcgcgga gcctcgcgag cagctcgccg cggccggcac
      361 cgttccgctt aagttccgcg agctcggcga cgaggctaaa aaaagccccc tcggcctctc
      421 ctatccatac ggcatcgagg atggccgaat acggcgtcgg gttcgtaatc gcaggaccac
      481 cggctatgac tatcggatgt tcgtccgttc ggtcggccga gcgtagcggc acgcccgaca
      541 ggtcgagcac cgacaggatg ttcgtggcgg ccaactcgta gccgaccgtg aaaccgagga
      601 tatcgaacga cgagacgggc gtacccgtct cgaggccgta gagcggggtc cttgactcgc
      661 gcagtagccg ctcgtaatcg gcggcgaccg cgaacacgcg ctcgcaggcg acgccgtcca
      721 agccgttcag cccggcgtac aggatacgca tcgcgttgtt ggccatgcct atctcgtaca
      781 ggtctgggaa gcacaggacc atcctgaacg gcgcgtcggg tttggcgatc gagccgctct
      841 cgccgcccag atacctcgcc ggcttggcta tcttgggtag ctcggtcttg aacaacgtgc
      901 atggatctac gcggctcaag agtcgtacct cctggccccg acgccgaggc atagtcccac
      961 ccctatgcag atgctccaca gcgacgaccc gccgtaagac aggaatagga gcggtatgcc
     1021 ggtgatcggc ataaccccca tcgccatgcc ggcgttaatc atgaaatgga agaagatgat
     1081 gccgaagacg ccggcgacga aggagctgga agccacgtcg cgcagtttgc gcattgaaag
     1141 cgccagacgc acgaggatat agaagaagag cccgaatatg acgatgccgc cgatgaaccc
     1201 gagctcctcg gagatgatgc tgaagatgaa gtcggtgctc tgctgaggca ggtagcggta
     1261 atggctctga gtcccctgta ggaaaccctt gccggcgacg ccgcccgagc ctatggcggt
     1321 gatggactgg agtatgttcc agcccgatcc gagcgggtcg atgcttgggt cgaggaacac
     1381 gatcagccgc ttgacctggt agtccttgag gaggcgctgg ccggccagcg agaaggccag
     1441 gccgagcgag ccgatagccg aaccgtaggc cagccagtag tagtagcgct tcttgaagaa
     1501 cctgaagccg agcagcgaga gcgccgccac caagacgagc atg
//
LOCUS       DAOOLN010000534         1543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_105740_length_1543_cov_2.806452, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000534 DAOOLN010000000
VERSION     DAOOLN010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105740_length_1543_cov_2.806452"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1543
                     /locus_tag="PLW80_07315"
     CDS             <1..>1543
                     /locus_tag="PLW80_07315"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HPB66352.1"
                     /translation="NYGRGTASTLIKASHELELGPYVGSVRLMVVAGTPDGAYGKAEL
                     ETPVRSELMAYLTAPRVLGPGETVTLPVTLLGFMGKGAEATVTVKLEGDAAVVGESKK
                     VVRFADEGEQSTSFDIAVADRVGAVSILVEAAGPGGRVSNQRVDVPIRSASVPVTTVS
                     SALIAGKATATVNAALPGMPGSNEAWLELSLVPPVDLSGRLAYLIGYPHGCGEQTVSK
                     AFPQLFLADAIALSPEQAEEARSNVAAAITKMAGFQTASGGFVFWQGSYDESAWLTAY
                     TTHFLAMARKQGYSVPDAMLGAAIAKIRGQASGWNAQADYSKAEQAYRLYVLALAGKP
                     DVASMNRFMEYGPHQAAALYQVAAAYALAGMSDRARSILRDGPADVTRYDGMRRVYGS
                     VLRDRAIVLDAFNALGDQARGLPLFKRIAEDLSSGSGYSTQDLSYALIASLPYMKVAS
                     SGAATVRYSYDGVSGSAAITKAITRVQLRAGADPLSVHLENASGAAVYARVAITGTPK
                     PGSEPY"
BASE COUNT          234 a          552 c          515 g          242 t
ORIGIN      
        1 caactacggg cgcggcaccg cctccacgtt gatcaaggcc agccacgagc tcgagctcgg
       61 gccgtacgtc ggctcggtgc gcctgatggt cgtcgccggc acgcccgacg gcgcctatgg
      121 caaggccgag ctcgagacgc cggttcgctc cgagctgatg gcctacctga ccgcgccgcg
      181 cgtgctcggc cccggcgaga cggtgacgct gccggtgacc ctgctcggct tcatgggcaa
      241 gggggccgag gctacggtca cggtcaagct cgagggcgat gccgccgtgg tcggcgaatc
      301 caagaaggtc gtgcgcttcg ccgatgaggg cgagcagtcg acctcgttcg acatagccgt
      361 cgccgaccgg gtcggcgcgg tcagcatcct ggtggaggcg gccggccccg gcggccgggt
      421 ctcgaaccag cgcgtcgacg tgcccatccg gtcggcctcc gtgcctgtca cgaccgtcag
      481 ctccgccctg atcgccggca aggctacggc caccgtcaac gcggcgcttc ccggcatgcc
      541 tggctcgaac gaggcctggc tcgaactgtc gctcgtgccg ccggtcgacc tgtccggtcg
      601 tctcgcctat ctcatcggct atcctcacgg ctgcggcgag cagacggtca gcaaggcctt
      661 cccgcagctg ttcctggccg acgccatcgc cttgagcccc gagcaggccg aggaggccag
      721 gtcgaacgtc gcggctgcca tcacgaagat ggcgggcttt cagaccgcct cgggcggctt
      781 cgtcttctgg cagggttcgt acgacgagag cgcctggctg acggcctata cgacccattt
      841 cctggccatg gccaggaagc agggctactc cgtgccggac gcgatgctcg gcgcggctat
      901 cgccaagata cgcgggcaag cctccggctg gaatgcccag gccgactatt cgaaggccga
      961 gcaggcctac cggctctacg tgctcgccct cgccggaaag cccgacgtcg cgtcgatgaa
     1021 ccgcttcatg gagtacggac cgcaccaggc cgcggccctg taccaagtag ccgcggccta
     1081 cgccctggcc ggcatgagcg accgcgccag gtcgatcctg cgcgacgggc ctgccgacgt
     1141 gaccaggtac gacggcatgc gccgggtcta cggctcggtg ctacgtgacc gagccatcgt
     1201 gctcgacgcg ttcaacgctc tcggcgacca ggcccgcggc ctgccgttgt tcaagcgcat
     1261 cgccgaggac ctgtcgtccg gctcaggcta ctcgacccag gacctgagct acgcgctgat
     1321 cgcctcgctc ccgtatatga aggtggcctc gagcggcgcg gctaccgtgc gctacagcta
     1381 cgacggcgtg tcaggtagcg ctgccatcac caaggccatt accagggtgc agctacgcgc
     1441 cggtgcggat cccctgtcgg tacacctcga gaatgcctca ggggccgcgg tctatgcccg
     1501 cgtcgcgatc accggtacgc ccaagccggg tagcgagccg tac
//
LOCUS       DAOOLN010000535         1542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_105833_length_1542_cov_3.478816, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000535 DAOOLN010000000
VERSION     DAOOLN010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105833_length_1542_cov_3.478816"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="PLW80_07320"
     CDS             <1..864
                     /locus_tag="PLW80_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66353.1"
                     /translation="GVDGALFGFPYVGIELLTGNLARLDVFGGRLFARPLVSTGMPIL
                     ENLQVGATMAMDRGVSNFASSETFSPVLAYGADVYLPLLTGQAFPLAAFSELAFEPKG
                     RTGFMVGAGGRLIGVITYAAQLRLLGAGFSPVYFDANYDLFRVQKAALMESDPTGEGM
                     AGWLAKAGASLFEDKLYFSVSVEGPFKATPAVASDSSAEYPHLRGVIGMAEGVLGGFF
                     VSGSYDKYFIGRENGFFADLVDPNDAVISAAFNYKTGAAVFTLLYNLSYNPEADKGFD
                     VTSSLQSSIKF"
     gene            1004..>1542
                     /locus_tag="PLW80_07325"
     CDS             1004..>1542
                     /locus_tag="PLW80_07325"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HPB66354.1"
                     /translation="MYPDSTVYSYATGSVMEGDAIPIGATVKTGADTSVELRLKPNGT
                     VVKLAKMTSFRVDGLATPQKDQNAFTIASGKIRAVAAKGSQYTIYTSTTVAGVRGTDF
                     SMSFEEGAKAMLLVAKGRVEFGRRGEGDAMADAIMVGAGQFADFFKGLTAARILRSSS
                     PRSTRVSIYRLNVYPSRPRT"
BASE COUNT          266 a          522 c          491 g          263 t
ORIGIN      
        1 ggcgtcgacg gggcgctctt cggcttcccc tacgtcggga tcgagctcct gaccggcaac
       61 ctggccaggc tcgacgtttt cggcggccgt ctcttcgcgc ggcccctcgt ctcgacgggc
      121 atgccgatcc tcgagaacct ccaggtcggc gcgacgatgg cgatggaccg aggcgtgtca
      181 aacttcgcct cgagcgagac cttctcgccg gtgctcgcct acggcgccga cgtctacctg
      241 cccctactga ccgggcaggc cttcccgctc gccgctttct ccgagctggc cttcgaaccg
      301 aaggggcgga ccggcttcat ggtcggcgcc ggtggccgcc tcatcggcgt catcacctac
      361 gcggcccagt tgcgcctctt aggcgccggc ttctcgccgg tctacttcga tgccaactac
      421 gacctgttcc gcgtccagaa ggccgcgctc atggagagcg acccgaccgg cgagggcatg
      481 gccggctggc tggccaaggc cggcgccagc ctcttcgagg acaagctgta cttcagcgta
      541 tcggtcgaag gccccttcaa ggcgacaccc gcggtcgcca gcgacagtag cgccgagtac
      601 ccgcacctgc gcggcgtgat cggcatggcc gagggcgtgc tcggcggctt cttcgtctcg
      661 ggctcgtacg acaagtactt catcgggcgg gagaacggct tcttcgccga tctcgtcgac
      721 ccgaacgacg cggtcatctc ggcggccttc aactataaga ccggagcggc cgtgttcacc
      781 ctgctctata acctgagcta caacccggag gccgacaagg gcttcgacgt gacctcgagc
      841 ctgcagagct cgatcaaatt ctgacggcaa ggggaggaac catatgcgcc cgataaaaac
      901 gatactggcg acggccttgg ccatcgcctt cgcggccgcc gcgttcgcgc aggctccgta
      961 atcgctcatc ctcgaattcg tcgacggcga caacctcgac gtcgtgtacc cggactcgac
     1021 ggtctatagc tatgcgacgg gatcggtgat ggagggcgac gccatcccga tcggcgcgac
     1081 ggtcaagacc ggcgccgaca cgagcgtcga gctacggctc aagccgaacg gcaccgtcgt
     1141 caagctggcc aagatgacga gcttcagggt cgacggtctg gcgacgcccc agaaggacca
     1201 gaacgccttc acgatagcct ccggcaagat cagggcggta gcggccaagg gctcgcagta
     1261 cacgatctac acctcgacca ctgtcgccgg cgtccgaggc accgatttct ccatgtcgtt
     1321 cgaggagggc gccaaggcga tgctcctcgt cgccaagggc agggtcgaat tcggcaggcg
     1381 cggagagggc gacgcgatgg cggacgccat catggtcggc gcgggccagt tcgccgactt
     1441 cttcaagggc cttacggctg cccgtattct gcggagcagt tcgccgagga gtactcgggt
     1501 ctcgatatac cgcttgaacg tatacccgag caggccgagg ac
//
LOCUS       DAOOLN010000536         1540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_106024_length_1540_cov_3.280808, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000536 DAOOLN010000000
VERSION     DAOOLN010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106024_length_1540_cov_3.280808"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PLW80_07330"
     CDS             complement(<1..727)
                     /locus_tag="PLW80_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPB66355.1"
                     /translation="MKAVELAKAYDPKEFEDRVYEAWQAEGHFKPVAPNGKQPFVVVI
                     PPPNVTGVLHMGHGLNNSMQDILVRFHRMNGQPTLWLPGTDHAGIATQHVVEKKLRAQ
                     GIDRRELGREKFVEETWKVTNEHHDVIKKQLEKIGSSCDWRRERFTLDDGLSKAVREV
                     FVTLYERGLVYKGEYLVNWCPSCGTALSDDEVEHADEAGAMYHIRYEIVDPPAGGPTH
                     IEIATTRPETLLGDTAVAYHPDDE"
     gene            837..>1540
                     /locus_tag="PLW80_07335"
     CDS             837..>1540
                     /locus_tag="PLW80_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPB66356.1"
                     /translation="MNAIDQYARLVVRVGVNLRKGQTLVVSSPIECADFARSIQKEAY
                     EAGAREVVFRWNDEKSARVTYDLAPDEIFDESPEWLRVFSDGYADAGAAFVSIAASDP
                     ELMKGVDPKRISRKNKANNVALEHYRSRLMSNRNAWCVASVPTEAWARKVFPGKSGPE
                     AMEALWDAIAKTVRVDQADPVAAWRAHQAALNARLAFLNGHAFKALRYRNAAGTDLTI
                     ALPAGHVWFGGGDDIVG"
BASE COUNT          247 a          510 c          499 g          284 t
ORIGIN      
        1 gctcgtcgtc cgggtggtag gcgacggccg tgtcgccgag cagggtctcg ggccgcgtgg
       61 tcgctatctc tatgtgggtc gggccgccgg ccggagggtc gacgatctcg tagcgtatgt
      121 ggtacatggc gcccgcctcg tcggcgtgct cgacctcgtc atcggacagc gcggtgccgc
      181 aggacgggca ccagttgacc aggtactcgc ccttatacac gaggccgcgc tcgtacagcg
      241 tcacgaacac ctcgcgcacg gccttggaca ggccgtcgtc gagcgtgaag cgctcgcgcc
      301 tccagtcgca ggaggagcct atcttttcta gttgcttctt gatcacgtcg tggtgctcgt
      361 tcgtgacctt ccaggtctcc tcgacgaact tctcgcggcc gagctctcgg cggtctatcc
      421 cctgggcgcg cagttttttc tcgacgacgt gctgggtggc gataccggcg tggtcggtgc
      481 ccggcagcca gagcgtcggc tggccgttca tgcggtggaa gcgcacgagt atgtcctgca
      541 tcgagttgtt caggccgtgg cccatgtgca gtacgccggt tacgttcgga gggggaatga
      601 cgacgacgaa cggctgtttc ccattgggcg cgacgggctt gaaatgccct tcagcctgcc
      661 aggcctcgta gacgcggtcc tcgaattcct ttgggtcgta ggccttggcc agttcgacgg
      721 ctttcatggg taggtgcctc cgatgcttct cgtagcggat actgtaccaa aaagctggcg
      781 gctgtgcaaa ggcccgaatc gtggtatcgt attcgcgccc gaataaagga gtctcaatga
      841 atgcgatcga tcaatacgcc aggctcgtgg tccgagtcgg cgtcaaccta cggaaaggcc
      901 agaccctcgt cgtcagttcg cctatcgaat gcgccgactt cgcccgctcg atccagaagg
      961 aagcctacga ggccggcgcc cgcgaggtgg tcttccgctg gaacgacgag aagtcggccc
     1021 gcgtcaccta cgacctggcc ccggacgaga tcttcgacga gtcgcccgag tggctacggg
     1081 tcttctccga cggctacgcc gacgccgggg ccgccttcgt cagcatcgcc gcttccgacc
     1141 ccgagttgat gaagggcgtc gaccccaagc gtatcagccg caagaacaag gccaacaacg
     1201 tagcgctcga gcactaccgc tcccggctga tgagcaaccg caacgcttgg tgcgtcgcgt
     1261 cggtgccgac cgaggcctgg gcccgcaagg tcttcccggg caagtccggc cccgaggcca
     1321 tggaggccct ctgggacgct atagccaaga cggtccgcgt cgaccaggcc gacccggtcg
     1381 ccgcgtggcg cgcccaccag gccgctctga acgccaggct cgccttcctg aacggccacg
     1441 ccttcaaggc cctgcgctac cgcaacgcgg ccggcaccga ccttacgata gccctgccgg
     1501 ccggccacgt ctggttcggc ggcggcgacg acatcgtcgg
//
LOCUS       DAOOLN010000537         1538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_106290_length_1538_cov_1.643965, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000537 DAOOLN010000000
VERSION     DAOOLN010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106290_length_1538_cov_1.643965"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /gene="dtd"
                     /locus_tag="PLW80_07340"
     CDS             <1..311
                     /gene="dtd"
                     /locus_tag="PLW80_07340"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPB66357.1"
                     /translation="KICGLRIFEDSDEKMNLSVVDTGGGVLSVSQFTLLADTRKGKRP
                     SYNDAAEPAVAEALYERFKEFVAAIVPTTASGVFGASMEVAYTNMGPVTILLDTKQPF
                     "
     gene            complement(427..1152)
                     /locus_tag="PLW80_07345"
     CDS             complement(427..1152)
                     /locus_tag="PLW80_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HPB66358.1"
                     /translation="MVELVDALERLLTAYGPRGWWPLPSLAGTPGRDGTGYALGPSLP
                     VGSGGGAIERSLRFEIAVGAVLAQNTSWSGAERAVATLVARSSAAGLPLSPQLLRSLA
                     GGELEDMLRPAGTFRIKAGYLRGLASAWSELDEGVPTRDGLLALPGIGFETADCVLLY
                     AYGVPAFVADAYARRLLLRLGYAQDSSSYEGARHYAEERLPHEAPFLAEAHALIVEHC
                     KLRCRSRPLCDGCPLATVCDHCR"
     gene            complement(1336..>1538)
                     /locus_tag="PLW80_07350"
     CDS             complement(1336..>1538)
                     /locus_tag="PLW80_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66359.1"
                     /translation="LMKHASDYGRKTKADLKLGICGEQGGDPATIDFCYRIGLNYVSC
                     SPFRVPIARLAGAQAVLRNAKK"
BASE COUNT          283 a          478 c          488 g          289 t
ORIGIN      
        1 aaaagatctg cggcctgcgc atcttcgagg actcggacga gaagatgaac ctgtcggtcg
       61 tcgataccgg cggcggcgta ttgtccgtgt cgcagttcac cctgctcgcg gatacgagga
      121 aaggcaagcg tccgtcgtac aacgacgcgg ccgagccggc cgtcgccgag gctctctacg
      181 agcgcttcaa ggaattcgtg gcggccatcg taccgactac cgcgtccggc gtcttcgggg
      241 catcgatgga ggtggcgtat accaacatgg gcccggtgac gatactgctc gatacgaagc
      301 aacccttcta gcccgccagg ttccgtaaca tagcgcgccg caatacgtcg ctccaagctt
      361 ctgggtcttc ttcatcgtta taccacgacg cttctaccgc tgcctctcga gttctcttcg
      421 ttctccttat cggcagtgat cgcagacggt cgcgagcggg caaccgtcgc acagggggcg
      481 cgagcggcaa cggagcttgc agtgctcgac tatgagcgcg tgagcctcgg cgaggaaggg
      541 tgcctcgtgc ggcagacgct cctcggcgta atgcctcgcg ccttcgtacg agctgctatc
      601 ttgcgcgtag ccgagcctta acagcagcct gcgcgcgtag gcgtcggcga cgaatgccgg
      661 aacgccgtag gcgtagagca ggacgcagtc ggcggtctcg aagccgatgc cgggtagggc
      721 cagaaggccg tcgcgggtcg gtacgccttc gtcgagctcc gaccaggccg aggctaggcc
      781 cctcaggtac ccggccttta tcctgaaggt gccagccggc ctcagcatgt cctcgagctc
      841 gccacccgcc aaagagcgga gcagctgtgg tgagaggggc agccccgcgg cagacgagcg
      901 ggctaccaag gtggctacgg cccgctcggc gcctgaccag gacgtgttct gcgcaaggac
      961 ggccccgacc gctatctcga agcgtagcga gcgttcgata gcgcctccgc cggacccgac
     1021 gggtaacgac gggcccaggg cgtacccggt gccgtcgcgg cctggcgttc cggccaggga
     1081 cggtagcggc caccagcctc ggggtccgta ggctgtgagt aatcgctcca atgcatcgac
     1141 cagttcaacc acgaatatgc tccagttaat aagacgacga agagtatacg agcatacgag
     1201 gtatgctaga gaagacccat tttaccgtta tgctctttcc tttctcttat tcacatatcc
     1261 tcttcgttcg cattagtaaa aaaaggcccg gctatccgtt cggatagccg ggccgttcat
     1321 cagctcaggg tcttcttact tcttggcgtt gcggaggacc gcctgggcgc cggccaacct
     1381 ggctatcggc acgcggaagg gcgagcacga tacatagttg agtccgatgc ggtagcagaa
     1441 gtcgatcgtc gcagggtcgc cgccctgctc cccgcagatg ccgagcttga ggtcggcctt
     1501 ggtcttgcgg ccatagtcgg aggcgtgctt catcagga
//
LOCUS       DAOOLN010000538         1537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_106362_length_1537_cov_2.446019, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000538 DAOOLN010000000
VERSION     DAOOLN010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106362_length_1537_cov_2.446019"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLW80_07355"
     CDS             complement(<1..184)
                     /locus_tag="PLW80_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66360.1"
                     /translation="MAGMTKVESYLMDLDIAYQELSPTSFYIDDTDKGLPGVIVSLDE
                     PIVTIRARAMQVPAGKR"
     gene            complement(231..776)
                     /locus_tag="PLW80_07360"
     CDS             complement(231..776)
                     /locus_tag="PLW80_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPB66361.1"
                     /translation="MPNNDIVPGFDDEKDDSLKIRLQKIDQNCLALFLTGYIDTYNSN
                     FFQKRVGKAIDAGFSRLIFNCGGLNYVSSTGIGSFTAFLKAVKPRSGDIVLLEIQPKV
                     YEVFQLLGFSQFFNIKDNLEEAVAYFQQGSQVSSQSVFPKIFSCPICTKKLKAAKPGR
                     FRCSECKTILAIDNSGQVFLG"
     gene            complement(957..>1537)
                     /locus_tag="PLW80_07365"
     CDS             complement(957..>1537)
                     /locus_tag="PLW80_07365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB66362.1"
                     /translation="KYGNEVGTIRADLSEGRAPPELLRAAASIDLLVVAYGPFVYKPL
                     AETSQADWSLAALGCLALPGTIATEAAVGMAGRGFGRILLFGGTRTDAVRGFKANAAY
                     AAAKTGLGVVAKSIAVEFGGRGVSCAVVCPGFVDTEYLDEAMRSRLEKASPRGGLIPA
                     AELASLVAYLLGGGMDLANGSVVVADRGLYAL"
BASE COUNT          333 a          441 c          443 g          320 t
ORIGIN      
        1 cccgcttacc ggccggaacc tgcatggcgc gcgccctgat ggttacgatc ggctcgtcga
       61 gggacacgat gacgccggga aggcccttgt ccgtatcgtc gatatagaat gaagtaggcg
      121 ataattcctg ataggctata tcgagatcca taagatagga ttcgactttc gtcatgcctg
      181 ccatgcgttc ctcctcgagg cgtcgtgcgg ataaggcgaa cctcggccga tcagccgagg
      241 aacacctgac ccgaattgtc gatcgcgagt atggtcttgc actcggagca acggaaacgg
      301 ccgggcttgg ccgccttcag cttcttcgta cagatagggc atgagaatat cttggggaac
      361 accgactgcg agctgacctg ggaaccttgc tggaagtacg cgacggcttc ctcgagattg
      421 tccttgatat tgaagaactg cgagaagccg agcaactgga agacctcata gactttaggc
      481 tgtatttcca gtaatacgat gtcgccagaa cgcggtttta cggccttgag aaaggccgtg
      541 aatgaaccga tgccggtcga cgatacataa ttgaggccgc cgcaattgaa gatcaggcgc
      601 gaaaagcctg cgtcgatggc cttgccgacg cgtttctgga agaaattaga gttatatgta
      661 tcgatatagc cggttaagaa cagcgccagg cagttctggt cgattttctg gagccgaatt
      721 ttaaggctat cgtccttctc gtcgtcgaag ccaggaacga tatcgttatt tggcatggtg
      781 ctcctcttcc atcgtgaggt ctattgtttt actgaaggtc aatactatga ccatcttagg
      841 ccgtcgtcaa tggcttgtca aatcgatact gacggcccga ccgatcgact gatcccttcg
      901 acgttatttt cggcaattta agtcgtccac gtaagctcta ttatatatcg cgcttgctac
      961 agagcgtata ggcctctatc ggcgacgacg accgaaccgt tcgcgagatc catgccgcca
     1021 cctaatagat acgctaccaa agaggccagc tccgcggccg ggatcagtcc gccccgtggg
     1081 gaggccttct cgagcctcga acgcatcgct tcgtcaaggt attcggtatc cacgaacccc
     1141 ggacatacga cggcgcacga gacgccgcgg ccgccaaact ccacggctat cgacttggcc
     1201 acgacgccca ggccggtctt ggcggccgca taggcggcat tagccttgaa gccacgtacg
     1261 gcatccgtcc tcgtgccgcc gaagaggagg atacgaccga agccgcgccc ggccatcccg
     1321 acggcggcct cggtcgctat cgtccccggc aaggccaggc acccgagcgc ggcgaggctc
     1381 caatcagcct gactggtctc cgcgaggggc ttatagacga agggcccgta ggcgacgacg
     1441 aggaggtcga tcgaggcggc ggccctcagc agctcgggag gagcccggcc ctcggatagg
     1501 tcggccctga tagtaccgac ctcgttccca tatttgg
//
LOCUS       DAOOLN010000539         1536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_106463_length_1536_cov_2.480756, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000539 DAOOLN010000000
VERSION     DAOOLN010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106463_length_1536_cov_2.480756"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /gene="frr"
                     /locus_tag="PLW80_07370"
     CDS             <1..435
                     /gene="frr"
                     /locus_tag="PLW80_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPB66363.1"
                     /translation="DKLRVDYYGQKSPISQVATVSVPEARLIVVQPWDRALIVEIEKA
                     IQKSELGLNPSNDGKVIRIAIPPLTEQRRKELVKSAKSSAETSRVGIRNIRRDGLEDI
                     KKAGAPEDEVKKAEDELQKLTDSFIAQINKVLEDKEKEILEV"
     gene            438..1136
                     /gene="uppS"
                     /locus_tag="PLW80_07375"
     CDS             438..1136
                     /gene="uppS"
                     /locus_tag="PLW80_07375"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HPB66364.1"
                     /translation="MQPTLARSVPAHVAVFMDGNGRWAKARGLPRTDGHREGLKAAKR
                     VVKAAKDAGVRYLTLYTFSTENWKRAQEEVKFLMGLVSTYLGAEFDFYRENGVRVVHS
                     GDDEGLPKDVREALERVSDDTAGFDGIVVNLAINYGGRDEILRAVSKLPDGVRAYSAE
                     EFRACLDLPDFPDPDLIIRAGGELRLSNFLLWQAAYSELYFSDVLWPEWNATHLEAAL
                     AEYARRERRYGDAR"
     gene            1133..>1536
                     /locus_tag="PLW80_07380"
     CDS             1133..>1536
                     /locus_tag="PLW80_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66365.1"
                     /translation="MSNFATRILFVGAAIPALFALATYVPFMDHLPLAILVLAFAAGS
                     GVELRRIVEPSAAKPRTVLAALFAALPSAVVYAVRQARPEAGLAASWLAPVAGVSIGL
                     FLISALPIALPRKADNVRASVAIATANAFYLL"
BASE COUNT          292 a          495 c          477 g          272 t
ORIGIN      
        1 gacaagcttc gcgtcgacta ctacggccag aaatcgccga tatcgcaggt agcgaccgta
       61 tccgtacccg aggctaggct catcgtcgtc cagccttggg atcgggcgct gatcgtcgag
      121 atcgagaagg ccatccagaa gtccgagctc ggcctcaacc cgtcgaacga cggcaaggtg
      181 atacgcatcg ccatcccccc gctcaccgag cagcgccgca aggagctcgt caagagcgcc
      241 aagtccagtg ccgaaacctc gcgggtaggc atccgcaaca tcaggcggga cggactcgag
      301 gacatcaaga aggccggagc tcccgaggac gaggtcaaga aggccgagga cgagcttcag
      361 aagctgaccg actcgttcat cgcccagatt aacaaggttc tcgaggataa agagaaggaa
      421 atcctggaag tctgagtatg cagcctacac tagcccgatc agttccggcg cacgtcgccg
      481 ttttcatgga cggcaacggg cgttgggcca aagctcgagg cttgccgagg acggatggcc
      541 atcgcgaggg gctcaaggcg gctaagcgcg tcgtgaaggc cgccaaggat gccggcgtcc
      601 gctacctgac gttgtatacc ttcagtaccg agaattggaa acgtgctcag gaagaggtta
      661 aattcctcat gggcctggtc agcacctacc tcggcgccga gttcgacttc tatcgcgaga
      721 acggagtgcg ggtcgtgcat tccggcgacg acgaaggcct gccgaaggat gtgcgcgaag
      781 cgctcgagcg cgtatcggac gacaccgcag gcttcgacgg catcgtcgtc aacctggcca
      841 tcaactacgg cggccgcgac gagatcctcc gggccgtttc gaagctcccg gacggggtgc
      901 gcgcgtatag cgccgaagaa ttccgcgcct gtctcgacct gcccgacttc cctgacccag
      961 atctgataat cagggctggg ggcgagctac gcctttccaa tttcctcctc tggcaggccg
     1021 catattcgga gttgtacttc tccgacgtgc tgtggcccga atggaacgct acccatctag
     1081 aagcggctct cgccgagtac gcccgcaggg agcgcagata cggtgacgct cgatgagtaa
     1141 cttcgctacc cgcatactgt tcgtcggcgc ggccatcccc gcgctgttcg ccctggccac
     1201 ctacgttccg ttcatggatc acctgccgct ggccatcctc gtactggctt tcgcggcggg
     1261 ctcgggcgtc gagctgcggc gcatcgtgga gccatcggcc gccaagccca ggaccgtcct
     1321 ggcggcgctg ttcgccgccc tgccaagcgc ggtcgtctac gccgtgaggc aggcccgtcc
     1381 cgaggctggc ctggccgcct cgtggctcgc gccggtggcc ggcgtctcga taggcttgtt
     1441 cctgatctca gccctgccca tcgcgctgcc gaggaaggcc gataatgtac gcgcctcggt
     1501 agcgatagcc acggccaacg cgttctatct gctgta
//
LOCUS       DAOOLN010000540         1536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_106474_length_1536_cov_2.302498, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000540 DAOOLN010000000
VERSION     DAOOLN010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106474_length_1536_cov_2.302498"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PLW80_07385"
     CDS             complement(<1..270)
                     /locus_tag="PLW80_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HPB66366.1"
                     /translation="MAAKSIDDTKRFATMVAAKQGWELNPDADFVGSLIKGLTVNWNR
                     YGYFLCPCRDSDGSREADASVICPCAYARDDVAERGHCFCALYLRA"
     gene            complement(273..572)
                     /locus_tag="PLW80_07390"
     CDS             complement(273..572)
                     /locus_tag="PLW80_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HPB66367.1"
                     /translation="MLETLSYIAVDGPRKVPVITVYALSTCGFCKRAMTFLQTNGFAY
                     RYIHMDAIPIEVKNEAKRALKDIYKADVAFPFATVGAKDYLVGFIEADWKRTLGV"
     gene            664..>1536
                     /locus_tag="PLW80_07395"
     CDS             664..>1536
                     /locus_tag="PLW80_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPB66368.1"
                     /translation="MHDIARTRNFCIIAHIDHGKSTLADRFIERAKLVDQRKMQQQML
                     DNMDIERERGITIKSQAVTLSYVARDGLEYTLNLVDTPGHVDFSYEVSRATSSCEGAI
                     LLVDATQGVEAQTLSNMYMAVEHDLEILPVINKIDMPAADVPEARRQIDHDLGLDGDE
                     ALCVSAKLGTGLDELFEAIVARVPAPSGSKDAPLRALIFDSHYDPYRGVIVHIRVFDG
                     SIVPGMAIRLMYNDAEYEVEETGLFKMGLVKTDGLVAGDVGYFIAGIKTVSDVRVGDT
                     VTGASDPVSEPLPGF"
BASE COUNT          283 a          528 c          465 g          260 t
ORIGIN      
        1 tgcgcgaagg tacagcgcgc agaagcagtg gccgcgctcg gccacgtcgt cgcgcgcgta
       61 ggcgcagggg cagatgaccg aggcgtcggc ttcgcgcgaa ccgtcggagt cccggcacgg
      121 gcataggaag tagccgtaac ggttccagtt gaccgtcagg cccttgatca ggctacctac
      181 gaagtcggcg tcagggttga gctcccagcc ctgcttggcc gcgaccatcg tcgcgaagcg
      241 cttcgtatcg tcgatgctct tagcggccac agctagactc ccagagtccg cttccagtcg
      301 gcctcgataa acccgacaag gtagtcctta gcgccgaccg tcgcgaaggg gaaggccacg
      361 tccgccttgt agatgtcctt gagcgcccgc ttcgcctcgt tcttgacctc gatcgggatg
      421 gcgtccatgt ggatgtagcg gtacgcgaag ccgttcgtct gcaggaaggt catggcgcgc
      481 ttgcagaagc cgcaggtcga cagcgcgtac acggtgatga ccggcacctt cctcggcccg
      541 tcgacggcga tatagctgag cgtttctaac atggcttcgt cctcctggga accatagtac
      601 gcccggagcc gccatttggc aaatcgattg aataactggc ccgattcggt atacattcgc
      661 gctatgcacg acatcgcccg aacccgcaat ttctgcatca tagcccatat cgaccacggc
      721 aagtcgaccc tggccgaccg cttcatcgag cgggccaagc tcgtcgacca gcgcaagatg
      781 cagcagcaga tgctcgacaa catggacatc gagcgagagc ggggcatcac catcaagagc
      841 caggccgtca ccctgtcgta cgtcgccagg gacgggctcg agtacaccct caacctcgtc
      901 gacaccccgg ggcacgtcga cttctcgtac gaggtgtcgc gcgccacaag ctcctgcgag
      961 ggcgccatac tcctcgtcga tgccacccag ggcgtcgagg cccagacgct gtcgaacatg
     1021 tacatggccg tcgagcacga cctcgagatc ctgccggtca tcaacaagat agacatgccg
     1081 gccgccgacg tgcccgaggc gcgccgccag atagaccacg acctcggcct ggacggtgac
     1141 gaggcgctct gcgtctcggc caagctcggc accggcctcg acgagctgtt cgaggccatc
     1201 gtcgcccggg tgcccgcgcc gagcggctcc aaggacgccc ccctgcgcgc cctgatcttc
     1261 gacagccact acgatcccta tcgcggcgtc atcgtccata tccgcgtgtt cgacgggtcc
     1321 atcgtccccg gcatggcgat ccggctcatg tacaacgacg ccgagtacga ggtcgaggag
     1381 accggcctgt tcaagatggg cctcgtcaag accgacggcc tcgtcgccgg cgacgtcggc
     1441 tacttcatcg ccggcatcaa gacggtctcc gacgtgcgcg tcggagacac ggtgaccggc
     1501 gcctccgacc ccgtctccga gccgctgccc ggcttc
//
LOCUS       DAOOLN010000541         1535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_106564_length_1535_cov_2.749324, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000541 DAOOLN010000000
VERSION     DAOOLN010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106564_length_1535_cov_2.749324"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PLW80_07400"
     CDS             <1..384
                     /locus_tag="PLW80_07400"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HPB66369.1"
                     /translation="SSSRKALDPSIVGAAPITTGCVMGDRRLVGDWLSSDRVSAFTVM
                     GSTKLDLRESVLPPGPIKIEAFTLMGEIQVIVPRDLPVKLNAFAFMGESNAGKEVNQQ
                     VRGAETWVEVSGFAMMGSLTVKARD"
     gene            complement(422..>1535)
                     /locus_tag="PLW80_07405"
     CDS             complement(422..>1535)
                     /locus_tag="PLW80_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HPB66370.1"
                     /translation="LPPENPEGVSLPLFAWAEYNLYHKSANKKRVKEVMPVLQRYYAW
                     LEATFKRPNGLFEVPVAATGMANSPRDGAAYLVDFNAAMAVNALYMSALGDILNDKEI
                     SFQYKRNYFSLKTRINNFMWNLDDGFYYDVDSAERQVRTKTIGGFWPLLAEIPNEERA
                     EAIIAHLTDPATFGTENPFPTVAVDSEHYDKRGMGYRGSVLPQFTYMVIKGLEKYNQW
                     ELARDCAIRHLYFMLDSAHQHGEHRQTLWEAYQPQNEGKAVWPDREDWPRFQYLGFAG
                     LATVAMMIENIVGLSVSLPRKTVDWIIPNLEIMGIENLSLKRNMVTILSNKSGRGWEI
                     HMESEKLYYFTINVIGKKKKTLPIPSGKCSMLIDKI"
BASE COUNT          259 a          466 c          492 g          318 t
ORIGIN      
        1 tcctcgtccc gcaaggcgct cgacccgtct atcgtcggcg ccgcgcccat taccaccggc
       61 tgcgtgatgg gtgatcgtcg cttggtcggc gattggctgt cgagcgaccg cgtatccgcc
      121 ttcacggtga tgggctccac caagctcgac ctacgggagt cggtgctacc ccccgggccg
      181 atcaagatcg aggcgttcac gctgatgggc gagatccagg tgatcgtgcc gagggacctg
      241 ccggtcaagc tcaacgcctt cgcgttcatg ggagagagca acgccggcaa ggaagtcaac
      301 cagcaggtac gcggagccga gacctgggtc gaggtgtcgg gcttcgcgat gatgggctca
      361 ttgacggtca aggcccggga ctaggggcgc gccttcggcc ggatcccgcc gaaggcggct
      421 ctcagatctt atctatcagc atcgagcact tgccggacgg gatcggaagc gtcttcttct
      481 tcttgccgat cacgttgatc gtgaagtagt agagcttctc cgactccatg tggatctccc
      541 aaccgcggcc gctcttgttg gacagtatcg tgaccatgtt gcgtttgagg ctcaggttct
      601 cgatacccat gatctcgagg ttcgggatga tccagtccac ggtcttccgc ggcagcgaca
      661 ccgaaaggcc gacgatgttc tctatcatca tcgcgacggt cgccaagccg gcgaagccca
      721 ggtactggaa ccgcggccag tcctcgcggt ccggccagac ggccttgccc tcgttctgag
      781 gctggtacgc ctcccagagc gtctggcggt gctcgccgtg ctggtgcgcc gagtcgagca
      841 tgaagtacaa gtggcggatc gcgcagtccc gcgccagctc ccactgatta tacttctcga
      901 ggcccttgat gaccatgtag gtgaactgcg gtagcacgga gcccctatag cccatgccgc
      961 gcttgtcgta gtgctcgctg tcgaccgcca cggtcgggaa gggattctcg gtgccgaacg
     1021 tggccgggtc ggtcaggtgc gcgatgatgg cctcggcgcg ctcctcattc gggatctcgg
     1081 ccaggagggg ccagaagccg cctatcgtct tcgtgcgcac ttgtcgttcg gcggagtcga
     1141 cgtcgtagta gaagccgtcg tcgaggttcc acatgaagtt gttgatcctg gtcttcagcg
     1201 agaagtagtt acgcttgtac tggaagctaa tttccttatc gtttaggatg tcgccgagcg
     1261 ccgacatgta cagcgcgttg acggccatcg ccgcgttgaa gtcgaccaga taggccgccc
     1321 cgtcgcgagg cgaattggcc atgcccgtag cggcgaccgg cacctcgaag aggccgttcg
     1381 gacgcttgaa cgtcgcctcg agccaggcgt agtaccgttg caggacgggc atcacttcct
     1441 tgacccgctt cttgttggcg cttttatggt agaggttata ctcggcccac gcgaacaggg
     1501 gcaggctgac gccttcggga ttctccggcg ggagc
//
LOCUS       DAOOLN010000542         1533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_106757_length_1533_cov_2.719215, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000542 DAOOLN010000000
VERSION     DAOOLN010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106757_length_1533_cov_2.719215"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..651)
                     /locus_tag="PLW80_07410"
     CDS             complement(31..651)
                     /locus_tag="PLW80_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HPB66371.1"
                     /translation="MTRKRAKRSAMLAAACGLAVVALLETAFGGRAASADEAARRATA
                     SADSELYTVPDRLRSLKALARRLGDMTSATVTRIVDGDTLVVELPGGSQEKVRLIGVD
                     TPESVDPRKPVERFSKEAAAYAERRLLGRDVRLAFGPEGRDYYGRLLAYVFLDDGTCV
                     NLAIVSDGYGFAYLKYPFPFQDEFSAAEREARRGGRGLWATSGETQ"
     gene            700..>1533
                     /locus_tag="PLW80_07415"
     CDS             700..>1533
                     /locus_tag="PLW80_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66372.1"
                     /translation="MDPVSPVLIAAAVVTLAAIVALGVSSGKHVRNASDFDTGGNKAG
                     PVMVAGTLVGTLVGGSATIGTAQLAFMYGLSAWWFTLGCSIGCVALGLGLARRMRASG
                     GGTIQEMISREYGGVARVVTSLLTSVGIVINIIAQILAANALLSTLFGLGPAASALVS
                     VGLMASYVLAGGIRGAGVLGLVKMALIYLTATVCGVLALRLSGGLGAIASALPADRYF
                     SLIARGLAVDLGAGLSVALGVLSTQTYAQAILTARSDGAAKNGALLSAIIVPPVGLLC
                     VF"
BASE COUNT          203 a          521 c          530 g          279 t
ORIGIN      
        1 ccgtatagaa gtgtcgtagc ggccagtagc ttactgcgtc tcgccgcttg tcgcccataa
       61 tccccggccg cctcgcctcg cctccctctc ggcggcggag aattcgtcct ggaacgggaa
      121 cgggtacttg aggtacgcga agccgtagcc gtccgatacg atcgccaggt tgacgcaggt
      181 tccgtcgtcc aggaatacat aggcgagcag gcgcccgtag tagtcgcgcc cctccggccc
      241 gaaggccagg cggacgtccc gcccgaggag gcgccgctcc gcgtaggccg cggcctcctt
      301 actgaagcgt tccaccggct tgcgcgggtc gacgctctcc ggggtatcga cgccgatcag
      361 cctgactttc tcctggctgc cgcccggcag ctcgacgacg agggtgtcgc cgtcgactat
      421 cctggtaacg gtcgcgctcg tcatgtcgcc gaggcggcgc gccagggcct tgagcgaacg
      481 gagcctgtcc gggacggtgt agagctcgga gtccgcggac gccgttgccc ggcgcgccgc
      541 ctcgtcggct gacgccgccc gtccgccgaa ggccgtctcc agcagggcga cgacggccag
      601 cccgcaggcg gccgctagca tcgcggatcg cttggctcgt ttcctggtca tcggcctatc
      661 gtatagccaa tcgaccggct cgtcgatatc attaccgcca tggatccagt atcaccggtt
      721 ctgatagccg cggcggtcgt cacgctcgcg gccatcgtcg ccctgggcgt atcgagcggc
      781 aagcacgtga ggaacgcctc ggatttcgat accggcggga ataaggccgg gccggtcatg
      841 gtcgccggta cgttggtcgg tacgctggtc ggcgggtcgg cgacgatagg cacggctcag
      901 ttggccttca tgtacggctt gtcggcttgg tggttcacgc tcggttgctc gataggctgc
      961 gtcgcgctcg ggctcgggct ggcgcggcgc atgcgggcct cgggcggcgg caccatccag
     1021 gagatgatca gccgagagta cggcggcgtc gcgagggtcg tcacttcgct cctgacctct
     1081 gtcggcatcg tcatcaacat catcgcgcag atcctggccg ccaatgcgct cctttcgaca
     1141 ctgttcgggc tcgggccggc ggccagcgcc ctcgtcagcg tcggcctgat ggcctcgtac
     1201 gtgctggccg gcggcatccg aggggccggc gtcctgggcc tggtcaagat ggcgctgatc
     1261 tacctgacgg cgacggtatg cggcgtcctg gccctccggt tgtcgggcgg cctgggcgcg
     1321 atagcctcgg cgctgccggc ggatcgctac ttcagcctga tcgcccgtgg cctcgcagtc
     1381 gatctgggcg ccggcctatc cgtggcgctc ggggtgctat cgacccagac ctacgcccag
     1441 gccatcctga cggccaggag cgacggggcg gcgaagaacg gcgcgctgct gtcggcgatc
     1501 atcgtgcccc cggtcgggct cctgtgcgtt ttc
//
LOCUS       DAOOLN010000543         1531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_107005_length_1531_cov_2.238482, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000543 DAOOLN010000000
VERSION     DAOOLN010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107005_length_1531_cov_2.238482"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PLW80_07420"
     CDS             complement(<1..451)
                     /locus_tag="PLW80_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66373.1"
                     /translation="MTPTKYVFVLGGDDPVILHPYDDPETFASAEPGSVVGRYGSGAA
                     PRGADGIRTALYAAMERGTRRYFLNRGYYLRLAMTASAFVAVYLFLSIVVRDPIPLID
                     ELLLGSLSAAAVFFASERKALSSPRHLDAVLRLRREIDGALFSESRVV"
     gene            complement(485..>1531)
                     /locus_tag="PLW80_07425"
     CDS             complement(485..>1531)
                     /locus_tag="PLW80_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66374.1"
                     /translation="SLSAEALALSSEDDDDLPLIPESLGLELVEETDLADVIGYIDSG
                     SSSDEEGGAGREAEAMIDTGESLADLRQERAEPEAPLPALVFASPIEEMAESVIGLDD
                     SAGLPADEASAVEAPAEEGNNEATAIAEDAVIAEDAVIAEDAVIAGRAAARESVVEAA
                     DDDDMAELLEEVEFDLFLSTLDLSGLEGYRDEDGFLELDDSSFEQQVFQRIAIAESQE
                     EELGDEITMLTDEQRARLEELQAPELEDDEAVDLEPLVGSTYRQIISAISYHRVEWPG
                     ATVEELPAVADGEPIDVMPLESVSREDADAPGEAIVMVDGVYTLNRDAVSNAVPEDQS
                     LKALAESVLTHRGA"
BASE COUNT          265 a          505 c          489 g          272 t
ORIGIN      
        1 cgacgacacg gctctcggag aaaagagcgc cgtcgatctc cctccggagc cgcagcacag
       61 cgtcgaggtg gcgtggcgac gatagggcct tgcgctcgct ggcgaaaaac acggccgccg
      121 cagacaacga gccgagcagt agctcgtcga tcagcggtat cgggtcgcgt acgactatgg
      181 acaggaacag atagaccgcg acgaaggccg acgcggtcat ggcaagccgt aggtaatagc
      241 ccctgttcag gaaatagcgc ctcgtgcctc gctccatggc cgcgtacagg gcggtacgga
      301 tgccgtcggc gccgcgcggc gccgcaccgg agccgtagcg gccaacgacg gaccccggct
      361 cggccgaagc gaaggtctcg ggatcgtcgt agggatggag tatgacggga tcatcgcctc
      421 cgagcacgaa gacatatttc gttggggtca tattgaagaa agtacgatcg gccggtcggc
      481 catgtcaagc gccgcgatgc gtcagcaccg actcggccaa ggctttcagc gactggtcct
      541 cgggtaccgc gttcgatacg gcgtcccgat tgagcgtgta cacgccgtcg accatcacga
      601 tcgcttcgcc gggcgcatcg gcgtcctccc gggagaccga ctcgagcggc atcacgtcga
      661 tcggctcgcc gtcggctacc gcgggcaact cctcgacggt agctccgggc cactcgaccc
      721 tatggtagga tatggcgctg atgatctgtc gataggtaga gccgacgagc ggttcgaggt
      781 cgaccgcttc gtcgtcctcg agctcggggg cctgaagctc ctccaggcgc gcccgctgct
      841 cgtcggtcag catcgttatc tcgtcgccga gctcctcttc ctggctctcg gcgatagcga
      901 tgcgttggaa cacctgctgt tcgaaggagc tgtcgtcgag ctcgaggaag ccgtcctcat
      961 cgcggtagcc ctcgagtccg ctcaggtcga gcgtactcag gaacaggtcg aattcgacct
     1021 cctcgagcaa ctcggccatg tcgtcatcgt cagccgcttc gaccacagat tcccgggccg
     1081 cggctcttcc ggcgatcaca gcgtcctcgg cgatcacagc gtcctcggcg atcacagcgt
     1141 cctcggcgat cgcggttgct tcgttattgc cctcttccgc tggggcctcg acagccgacg
     1201 cctcgtcggc cggtaaaccg gcgctatcgt cgaggccgat gaccgactcg gccatttcct
     1261 cgatcggtga agcaaacacg agggccggta gaggcgcctc aggctcggcc cgttcctgcc
     1321 tgaggtcggc cagcgactcg ccagtatcga tcatggcctc ggcctcacgc ccggcgccgc
     1381 cctcttcgtc gctcgatgag ccggaatcga tatacccgat gacgtcggcg aggtcggtct
     1441 cctccacgag ctccagcccg aggctctcgg gtatgagcgg gaggtcgtca tcgtcttccg
     1501 aggacagcgc tagagcctct gcagagaggc t
//
LOCUS       DAOOLN010000544         1529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_107179_length_1529_cov_2.932157, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000544 DAOOLN010000000
VERSION     DAOOLN010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107179_length_1529_cov_2.932157"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PLW80_07430"
     CDS             <1..387
                     /locus_tag="PLW80_07430"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPB66375.1"
                     /translation="GYCRLRGDYRLFRASRIRRPRPLPERFARRPKTFEEFEREYDPA
                     HTGSLVEVKLRFAPEMAPLVEEFHNEEDLERLEDGSIVARMAMPEDGWMYGYVLSYGQ
                     FVELLEPERLRAVIRDAAGKIRSLYE"
     gene            470..916
                     /locus_tag="PLW80_07435"
     CDS             470..916
                     /locus_tag="PLW80_07435"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HPB66376.1"
                     /translation="MRIIECAERDALSVRYRTPVAKLSESMGPAYGEIAGYMAKNGIP
                     FAGPPYAMYYNMDMDDLDVEIGFPVGAPAKGEGRVRPGKLPGGRHATATHGGSYETMA
                     KTYDALTAFCKERGVELEERMYEEYLNSPEEVPPEKLETVIHFLLR"
     gene            1065..>1529
                     /locus_tag="PLW80_07440"
     CDS             1065..>1529
                     /locus_tag="PLW80_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66377.1"
                     /translation="MPMTVFVDSILESNLGYAALFICVGVGFLFLRQVDSGEPGPGLW
                     SLGFFLNALGFLLWSGTVAIPTVLYLLAGEVFHVAGFMMMITGLYRFTGRAYTPAFFA
                     LVAAWLLIWAVSIVMLRYFVYPAGIALKLLRAVLFVVAGFMALDRRGDGSRFG"
BASE COUNT          255 a          497 c          499 g          278 t
ORIGIN      
        1 ggctactgca ggctcagggg cgactaccga ctgttccgcg cgtcccgcat ccgaaggccc
       61 cgcccgctgc ccgagcgctt cgcgaggcgg cctaagacgt tcgaggagtt cgagcgcgag
      121 tacgacccgg cccataccgg aagcctcgtg gaggtcaagc tgcgcttcgc gccggagatg
      181 gcccccctcg tcgaggagtt ccataacgag gaggacctgg agcgcctcga ggacggctcg
      241 atcgtcgcca ggatggccat gccggaggac ggctggatgt acggctacgt cctgtcctac
      301 ggccagttcg tcgagcttct cgagcccgag cgcctacgcg ccgttatccg cgacgcggcc
      361 gggaaaattc gctcgttata cgaataggcg gtacgattaa ccccgacata gaggtgtcgt
      421 atatcccgcg ttaccatgcg tccatcctgg ttccagaagg agagcgagca tgaggatcat
      481 cgaatgcgcc gaacgcgacg cgctatcggt acgctaccgg acgccggtcg ccaagctgtc
      541 ggagagcatg ggtcccgcct acggcgagat cgccggctac atggccaaga acggcatccc
      601 gttcgcgggg ccgccctacg ccatgtacta caacatggac atggacgacc tggacgtgga
      661 gataggcttc ccggtaggcg ccccggccaa gggcgagggc cgcgtgaggc cgggcaagct
      721 acccggcggg cggcacgcga ccgccacgca cgggggctcg tacgagacca tggccaagac
      781 ctacgatgcg ctgacggcct tctgcaagga gcggggcgtc gagctcgaag agcgcatgta
      841 cgaggagtac ttgaacagcc ccgaggaggt gcctccggag aagctcgaga cggtcatcca
      901 cttcctgctc aggtgagcgc tagcccggcg gcgtaccgtc gccgccgggc tagtgctgaa
      961 acgccgagca tgctagtatc gacgtattct gtcgaccgcg gatcgttccg cgcctccagc
     1021 ctagctaaaa gtgattacca gacacctagg gcaaggagcg gcgtatgccg atgaccgttt
     1081 tcgtcgattc tatcctggag agcaacctcg ggtacgccgc gctgttcatc tgcgtgggcg
     1141 tgggcttcct gttcctcagg caggtggact cgggcgaacc gggccctggg ctctggtcgc
     1201 tcggcttctt cctgaacgcg ctcggcttcc tgctctggtc gggtacggtg gcgataccga
     1261 ccgtcctgta cctcctggcg ggcgaggtct tccatgtcgc cggcttcatg atgatgataa
     1321 ccgggctcta tcggttcacc ggccgcgcct acacgccggc tttcttcgcg ctcgtcgccg
     1381 cctggctcct gatctgggcc gtatccatcg tcatgctccg gtacttcgtc tacccggcgg
     1441 gcatcgcgct caagctatta cgggccgtac tgttcgtcgt cgccggcttc atggcgctcg
     1501 accggcgcgg ggacggctcg cgcttcggc
//
LOCUS       DAOOLN010000545         1525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_107644_length_1525_cov_2.406803, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000545 DAOOLN010000000
VERSION     DAOOLN010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107644_length_1525_cov_2.406803"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="PLW80_07445"
     CDS             <1..1293
                     /locus_tag="PLW80_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66378.1"
                     /translation="LGDSFKADVAAISDNWEKERRKVIEAAKLERDALSKDVRALSDE
                     VGRFRQELAQKTSQALDDFNRSYDGMAQDAARRARESVTAMGAAVEDYRREARLLQEG
                     FDAAKAQMAGSLESERKARVKAFEEMDGQIKAFQAQTKLFERADELKASLGEAVEAMK
                     ADLARVESRRAEMAELETQYSRVKRLEDELGQKIAKFLAEKRRLDAMEDDFKRLLALS
                     QTVDQKLASVTSNNDQLTQLEAEFRRVVDAADEAAEKYERVEKKSNILNATADAIDKN
                     FQAIGELERNVRTMDADIRELPDRVIDLKRSLDEVMSWKPKLDATVVRLDEVDGVLAD
                     AEKRATELQKAREWLARAETRFDELNKKTQDHLKLLNDLLKDEPSAGRKDKGAPPLSV
                     QETVRKLSHQGWKVEEIARAVKLSRGEVELILELGGQD"
     gene            complement(1320..>1525)
                     /locus_tag="PLW80_07450"
     CDS             complement(1320..>1525)
                     /locus_tag="PLW80_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66379.1"
                     /translation="EGSIVYRDNLTELGWGATAGVTIPVWKLELDANYTLRQRPSRSL
                     PGVTIPENVFFITVSADGLLFGD"
BASE COUNT          310 a          501 c          514 g          200 t
ORIGIN      
        1 ctcggcgact cgttcaaggc ggacgtcgcg gccatctccg acaactggga gaaggagcgc
       61 cgcaaggtta tcgaggcggc caaactcgag cgcgacgccc tctcgaagga tgtgcgcgcg
      121 ctgtccgacg aggtcggccg cttccgacag gagctcgccc agaagacgtc ccaggccctg
      181 gacgacttca accgctccta cgacggcatg gcgcaggacg ccgctcgcag ggccagggaa
      241 tcggtcacgg ccatgggcgc cgccgtcgag gactaccgcc gcgaagcccg cctgctccag
      301 gaaggcttcg acgccgccaa ggctcagatg gccggctcgc tcgagtcgga gcggaaggcc
      361 cgcgtgaagg ccttcgagga gatggatgga cagatcaagg cgttccaggc ccagacgaag
      421 ctgttcgagc gggccgacga gctcaaggcg tcgctcggcg aggcggtcga ggcgatgaag
      481 gccgacctgg cccgcgtcga gtcgcgccgg gccgagatgg ccgagctcga gacccagtac
      541 agccgcgtca aacggctcga ggacgagctc ggacagaaga tagccaaatt cctcgccgag
      601 aagcgccgcc tcgacgccat ggaggacgac ttcaagcgcc tcctggccct gtcgcagaca
      661 gtcgaccaga agctggcctc ggtcacctcg aacaacgacc agctcacgca gctcgaggcc
      721 gagttccgcc gcgtggtcga cgcggccgac gaggcggccg agaagtacga gcgcgtcgag
      781 aagaagtcga acatcttgaa cgctaccgcg gacgctatag acaagaattt tcaggccatc
      841 ggcgagctcg agcgcaacgt caggacgatg gacgccgaca tccgggagct acccgaccgc
      901 gtcatcgacc tcaagcgatc cctcgacgag gtaatgtcct ggaagcccaa gctggacgcc
      961 acggtggtca ggctcgacga ggtggacggc gtgctggccg acgccgagaa gcgggcgacc
     1021 gagctccaga aggcccgcga atggctcgcc cgcgccgaga cgcgcttcga cgagctcaac
     1081 aagaagaccc aggaccacct taagctactc aacgacctgc tcaaggacga gccgagcgcc
     1141 gggcgcaagg acaagggcgc gccgcccctg tcggtccagg agaccgtgcg caagctgtcc
     1201 caccagggct ggaaggtcga ggagatagct cgcgccgtca agctgtcgcg cggcgaggtc
     1261 gagctgatcc tggagctggg cgggcaggac tagcggggcc cccacgcccc gccctccgtt
     1321 taatcgccga acagcaggcc atccgccgaa accgtgatga agaagacgtt ttcggggatg
     1381 gtgactccgg gcagagatcg cgagggccgt tgcctgaggg tgtagttcgc gtccagttcg
     1441 agcttccata ccggtatcgt cacgccggcc gtggcccccc agccgagctc ggtcagattg
     1501 tcccgataca cgatcgagcc ttcga
//
LOCUS       DAOOLN010000546         1524 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_107762_length_1524_cov_2.161334, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000546 DAOOLN010000000
VERSION     DAOOLN010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107762_length_1524_cov_2.161334"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="PLW80_07455"
     CDS             <1..912
                     /locus_tag="PLW80_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HPB66380.1"
                     /translation="EEISFIADDGKSIHVYHWAPEGEVKACVLVAHGMAEHAARYARL
                     ARSMTKAGYELWAPDHRGHGKTAAEGELGWLADTDGFRRVIEDLRGLAARIKADHPGK
                     KLFLLGHSWGSFLSQGFISLYGDELAGCALSGTAGDGGAVIGMGRSIAKLGCALRGQK
                     AKAPLMNAMNFGSYNKAFKPNRTVFDWLSRDEAEVDAYIADPFCGFLCTYGFFRDLLG
                     GLAWIHAPATMAGVPTSLPIYMFAGAKDPVGAANGSFDWLYNRYKELGVVDLSRKLYP
                     DGRHEVFNDTCRDEATADVIAWLDARA"
     gene            1039..>1524
                     /locus_tag="PLW80_07460"
     CDS             1039..>1524
                     /locus_tag="PLW80_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HPB66381.1"
                     /translation="MLVRYQNGPDGKPVKKAIVYTEREHGIDPRSVDQDAVRIVERLR
                     RSGHAAYIVGGAVRDLLLGREPKDYDIVTDAEPPRIKKLFYRSRIIGKRFRLVHVYVG
                     ERIFEVSTFRSIANGTVGNTYGTIDEDAKRRDFSMNALYFDPLEQKLVDYVGGLKDMR
                     AR"
BASE COUNT          285 a          493 c          479 g          267 t
ORIGIN      
        1 gaagaaatct cgtttatcgc cgacgacggc aagagtatcc atgtatacca ttgggctccc
       61 gaaggggagg ttaaggcttg cgtgctcgtc gcgcacggca tggcggagca tgccgcccgg
      121 tacgcccgcc tggctaggtc gatgaccaag gccggctacg agctatgggc ccccgatcat
      181 cgcggccacg gcaagaccgc cgccgagggt gaactcggct ggctggcgga tacggacggg
      241 ttcaggcgcg tcatcgagga cctgcgggga ctcgcggccc ggatcaaggc cgatcacccg
      301 ggcaagaagc tgttcctgct cgggcattca tggggctcgt tcctgtcgca gggcttcatc
      361 tcgctgtacg gcgacgagct ggcgggttgc gcgctcagcg ggacggccgg ggacggcggc
      421 gccgtcatcg gcatggggcg ctcgatcgcg aagctcggct gcgccctgcg cggccagaag
      481 gccaaggccc cgctcatgaa cgcgatgaac ttcggttcgt ataacaaggc cttcaagccg
      541 aaccgcacgg tattcgattg gctgtcgcga gacgaagccg aggtcgatgc atacattgcc
      601 gaccccttct gcggtttcct atgcacctac ggttttttca gggacctgct cggaggcctg
      661 gcttggattc atgctccggc cacgatggcg ggcgtaccca cgtcgttgcc catctatatg
      721 ttcgccgggg ccaaagaccc tgtcggggcc gccaacggct ccttcgactg gctgtacaat
      781 cgctacaagg agctgggcgt cgtcgaccta tccaggaagc tataccccga cggccgtcac
      841 gaggtgttca acgacacctg ccgcgacgag gcgaccgccg atgtcatagc ctggctcgac
      901 gccagagcct gaccgaggca acgccgtagc cccgtaccgg cttgacctgt accgccggtg
      961 cgggggtata gtacagtacc cgggccagcg gcccagtcgc tctcgtagcg atccgcacat
     1021 ctatctctag aggaaacctt gctcgtacga tatcagaacg ggccggatgg aaagccggtc
     1081 aagaaagcca tagtctatac ggaacgcgaa cacggcatcg atccgcgttc cgtggatcag
     1141 gacgcggtgc gcatcgtcga gcgcctgagg cgctccggcc acgcggctta catcgtcggc
     1201 ggcgccgtcc gggacctgct cctcggccgc gaacccaagg actacgacat cgtcaccgac
     1261 gccgagcccc ctcggatcaa gaagctgttc tatcgctcga ggatcatcgg taaacgcttc
     1321 cggctcgtcc atgtctacgt cggcgagcgc atcttcgagg tctcgacctt ccgttcgata
     1381 gccaatggca ccgtcggcaa cacctatggc accatcgacg aggacgcgaa acgccgcgat
     1441 ttcagcatga acgcgctgta cttcgacccg ctcgagcaga agctcgtcga ctacgtcggc
     1501 ggcctcaagg acatgcgcgc cagg
//
LOCUS       DAOOLN010000547         1521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_108041_length_1521_cov_3.002046, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000547 DAOOLN010000000
VERSION     DAOOLN010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108041_length_1521_cov_3.002046"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PLW80_07465"
     CDS             <1..709
                     /locus_tag="PLW80_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB66382.1"
                     /translation="EPVLDRVSFTIPKGETVALVGPSGSGKTTTVSLLCGFYAATSGS
                     ILVDDAPLGELSTQAWRRTIGLVLQDVYLFPGSILENVRVYDDGLSVEDAEAALRTVH
                     AADFVSRLPGGIGAELHERGSNLSMGEKQLLSFARAVAFGAELIIMDEATASVDVATE
                     RKIQRSLDVLLKGRTALIVAHRLSSILKADKILFFKDGRIIAQGRHDELIESLPEYRE
                     LVRLQFPDLIAETETIA"
     gene            706..>1521
                     /locus_tag="PLW80_07470"
     CDS             706..>1521
                     /locus_tag="PLW80_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB66383.1"
                     /translation="MSEPSGGADAPRVDHIRWIGRLWKEKAWLIVVLFALTLLSSAVA
                     VTYPYLCKRLLDTIQRLLESGADDAMREVDRLLLVFAAVGVGGLVASLFPGIRGVTNS
                     IFEHAIRSKYWRKVLAKDYSFFSKFSSGDIVTRLTDDLYDFPKLSWFLCSGIFRAVES
                     ISKVAFCLGAMFLLDWRLTLLSLIPLPVMIALFYVTQDRIYDTFQKNQQAISAINSRL
                     EMSFSGVRIIKAYACEDKYKRFFSDVLKTRWNTEMAVARLEVVLHMIYQYIDYV"
BASE COUNT          274 a          502 c          456 g          289 t
ORIGIN      
        1 cgagccggtg ctcgaccgcg tctcgttcac catccctaag ggcgagacgg tagccttggt
       61 cggcccgtcc ggctcgggca agacgacgac ggtcagcctg ctatgcggct tctacgccgc
      121 gacctccggc tcgatactcg tcgacgacgc gccgctcggc gagctgtcga cccaggcctg
      181 gaggcgcacg ataggcctcg tcctccagga cgtctaccta ttcccgggta gcatcctcga
      241 gaacgtcaga gtctacgacg acggcctgtc cgtcgaggat gccgaggcgg cgctccgcac
      301 ggtgcatgcc gcggacttcg tctcaaggct gcccggcggc ataggcgcgg agttgcatga
      361 gcggggctcc aacctatcga tgggcgagaa gcagctcctg tccttcgcca gggccgtcgc
      421 gttcggcgcc gagctgatca tcatggacga ggcgacggcg agcgtcgacg tggcgaccga
      481 gcgcaagatc cagcgtagcc tggacgtgct gctaaagggc cgcaccgcgc tgatcgtggc
      541 gcaccgcctc tcgtcgatcc tcaaggcgga caagatactg ttcttcaagg atggtcgcat
      601 catcgcgcag ggcaggcacg acgagctgat agagtcccta cccgaatatc gggagctggt
      661 acgcctccag ttccccgacc tgatagccga aacggagacg atagcatgag cgaaccgagc
      721 ggcggcgccg acgcgccgcg cgtagaccat atacgttgga taggccgact ctggaaggag
      781 aaagcctggc tgatcgtcgt cctcttcgct ctgaccctgc tgtcgagtgc cgtggccgtc
      841 acctatcctt acctctgcaa gcgcctcctc gacacgatcc agcgcctcct cgagtccgga
      901 gcggatgacg ccatgcgcga ggtcgaccgg ctgctcctcg tgttcgccgc ggtcggggtc
      961 ggcggcctcg tcgcctcgct cttcccgggc atacgcggcg tgaccaactc catcttcgag
     1021 cacgctatac gctccaagta ctggcgcaag gtactggcca aggactactc cttcttctcc
     1081 aagttctcct ccggagacat cgtcacgagg ctgaccgacg acctgtacga tttccccaag
     1141 ctgtcatggt tcctctgctc cggcatcttc agggcggtcg agtcgatcag caaggtggca
     1201 ttctgcctgg gcgccatgtt cctgctcgac tggcggttga ccctgctgtc gctgataccg
     1261 ctcccggtca tgatcgccct gttctacgtg acgcaagata ggatctacga taccttccag
     1321 aagaaccagc aggcgatcag cgctatcaac tcgaggctcg agatgagctt ctccggcgtg
     1381 cgcatcatca aggcctacgc ctgcgaggac aagtacaagc gcttcttctc cgatgtactc
     1441 aagactcgtt ggaacaccga gatggccgtg gccaggctcg aggtcgtgct ccacatgatc
     1501 taccagtaca tcgactacgt g
//
LOCUS       DAOOLN010000548         1520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_108127_length_1520_cov_3.782935, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000548 DAOOLN010000000
VERSION     DAOOLN010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108127_length_1520_cov_3.782935"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="PLW80_07475"
     CDS             complement(<1..979)
                     /locus_tag="PLW80_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HPB66384.1"
                     /translation="MNDILVHSEAVLGYGFVADKYLEDGESEYRLRNAQLDKPSDSYR
                     PLRHLDVEILIKNGNRCSDWGKLLVREPFDATLIRNSEFAGLVRIGSLERVVLEHHDM
                     MIPAGITNSRIISCDIGDQCAVHSCIYLAHYIVGDRCILMNNDDIHVSDHAKWGNGVI
                     KQGESEDVRIRLDLMNEAGGRSVLPFDGMLCADAYLWAKRRDDAALMERFEEMTDAAF
                     EHRRGEYGVIGSGSVLKSNRIIKDVRVGECAYIKGANKLKNLTINSSDAERTQIGEGV
                     ELVNGIVGYGCRVFYGCKAVRFVMGTNSALKYGARLIHSVLGDNSTVSCC"
     gene            1157..>1520
                     /locus_tag="PLW80_07480"
     CDS             1157..>1520
                     /locus_tag="PLW80_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66385.1"
                     /translation="MGAPVYAVDDPADLDVEKAAFELFPLLVGTDNSVLRREYGSALA
                     DIIGGPGAFRKYVVGNTGDLALCRARLLERFRDNVRLLVAKTWVDGKDEVGKSEALAL
                     LDSFVGMVDAGDYPNAVPA"
BASE COUNT          260 a          491 c          488 g          281 t
ORIGIN      
        1 cgcagcacga gaccgtcgag ttgtcgccga gcaccgagtg tatcagcctg gccccgtact
       61 tgagcgcgga attcgtgccc atcacgaagc gcacggcctt gcagccgtag aacacgcggc
      121 aaccgtagcc gacgatgccg ttgaccagct cgacgccctc gcctatctgg gtacgctcgg
      181 cgtcgctcga gttgatcgtc aggttcttga gcttgttggc gcccttgatg taggcgcact
      241 cgcctacccg cacgtccttg atgatgcggt tgctcttgag cacgctaccg gagccgatca
      301 cgccatactc gcctcgccgg tgctcgaagg cggcgtcggt catctcctcg aagcgctcca
      361 tgagcgcggc gtcgtcgcgg cgcttggccc agaggtaggc gtcggcgcag agcataccgt
      421 cgaagggcag cacggaacgg ccgccggcct cgttcatcag gtcgagccgg atacgaacgt
      481 cctcgctctc gccctgcttg atgacgccgt tgccccattt ggcgtggtcg gagacgtgga
      541 tatcgtcgtt gttcatgagg atgcagcggt cgccgacgat atagtgcgcc aggtagatgc
      601 acgagtgcac ggcgcactgg tcgccgatgt cgcaggaaat gatgcgcgag ttcgtgatac
      661 ccgcagggat catcatgtcg tggtgctcga gcaccacgcg ctcgaggcta ccgatgcgaa
      721 ccaggccggc gaactcgctg ttgcgtatca gggtggcgtc gaacggctcg cgcacgagta
      781 gcttgcccca gtcggagcag cggttgccgt tcttaatgag tatctcgacg tcgagatggc
      841 gcagggggcg atacgagtcg gacggtttgt cgagctgggc gttcctgagc ctatactcgc
      901 tctcgccgtc ctcgaggtac ttgtcggcga cgaacccata gcccaggacg gcctcggagt
      961 gtacaaggat gtcgttcatg gcggccttcc agacgttcgg taatggggag cgagcctccc
     1021 ctcgacctta atcgtaaccg ctaatgccta gcttttcaac acacggccga gccccgaggc
     1081 agctcagcgc cgtcgcgggg gaccgaccga accggtatta ttgacaaagc ggcggagggc
     1141 gtgacatggt tgccccatgg gcgcacccgt gtacgcggtc gacgatccgg cggacctgga
     1201 cgtcgagaag gccgccttcg aactcttccc cctcctcgtc ggcacggaca actcggtgct
     1261 tcgccgcgag tatggatcgg ccctggccga catcatcggc ggcccggggg ccttcaggaa
     1321 atacgtagtc ggcaacacag gcgacctggc cctctgccgc gcccgcctcc tggaacgctt
     1381 ccgcgacaac gtacggctcc tcgtcgccaa gacctgggtg gacggcaagg acgaggtcgg
     1441 caagtccgag gccctcgccc tgctcgactc cttcgtcggc atggtggacg caggcgacta
     1501 cccgaacgcc gtgcccgcct
//
LOCUS       DAOOLN010000549         1519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_108234_length_1519_cov_3.586066, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000549 DAOOLN010000000
VERSION     DAOOLN010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108234_length_1519_cov_3.586066"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PLW80_07485"
     CDS             <1..300
                     /locus_tag="PLW80_07485"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HPB66386.1"
                     /translation="DPTTEDRQGADIGTQYRSIILYADEAQKKAAEAAIREAAPRYAR
                     PIVTELEALGAFWPAEDYHQDFYRKNPNYGYCRAVVAPKVEKARSFVDQLVPAKP"
     gene            complement(660..>1519)
                     /locus_tag="PLW80_07490"
     CDS             complement(660..>1519)
                     /locus_tag="PLW80_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB66387.1"
                     /translation="RGLGEILVFKKIKTKLGGRFKAGVSGGGAMPASVDIFFDTIGVQ
                     ILEGYGLTETAPVIGLRPQRKPVLGTVGPAIRGTEFRITDEAGIDVGRCRKGVVHVRG
                     PQVMLGYYKRPELTAKVLSDDGWLNTGDLGMLTRQGFLKLTGRAKDTIVLRGGENVEP
                     LPIEQRICDSPYVKQAVVLGQDQRFLAALIVPDQDAVIAWAKDTTVPIVDYEALLAQP
                     EVRELFDYEIGQAVNQKTGFKSFERIFRFELLPEPFEVGRELSAKQEIKRHVVAELYK
                     QRIARMFRD"
BASE COUNT          287 a          464 c          470 g          298 t
ORIGIN      
        1 gacccgacga ccgaagacag acagggcgcg gacattggca cccagtatcg atccatcatc
       61 ctctacgctg acgaggctca gaagaaggcg gccgaggccg cgatacggga agccgctccc
      121 cggtacgctc ggcctatcgt caccgagctg gaagcgctcg gcgcgttctg gcccgccgag
      181 gactaccatc aggacttcta ccgtaaaaac ccgaattatg gctactgccg cgccgtcgtg
      241 gcgcccaagg tcgagaaggc caggtctttc gtcgaccagc tcgtgcccgc caagccgtag
      301 taggcgaata cgggcgaaga tcggggaagt tgaaccacgg agacactgag gcgcggagaa
      361 gaggagcgcg atgggggagg ggggcggagc catggctttg cccttggtcg gctccttgga
      421 tcggcttcct ctgtgccgta attgctcaat ctgaggccgt cgaataagaa atttcccgca
      481 gaggccgtat aggcgaagac gagaacatga tgctacggga tggaagaaga tggacatgat
      541 gaagataagt ccaggtctaa gacctatctt gttcatcctg tccaaatctt gataatcctg
      601 ttctatctta accctattcc tctgtgcctc tgcggcagat tcttcgtaat ccggtcggat
      661 cagtcgcgga acatcctggc tatgcgctgc ttgtacagct ccgcgaccac gtggcgcttg
      721 atctcctgct tcgccgatag ctctcgtccg acctcgaacg gctcgggcag cagttcgaac
      781 ctgaagatgc gctcgaagct cttgaagccc gttttctggt tgacggcctg gcctatctca
      841 tagtcgaaca gctccctaac ctcgggctgg gcgagcagag cctcatagtc gacgataggc
      901 acggtcgtat ccttggccca ggcgatgacg gcgtcctggt caggcacgat cagcgcggcg
      961 aggaaacgct ggtcctgccc gagcacgacg gcttgcttga cgtaggggct gtcgcagatg
     1021 cgctgctcga tgggtagcgg ctcgacgttc tcgccgccgc gcagcacgat ggtgtccttg
     1081 gcccgtccgg tcagcttgag gaagccctgc ctggtcagca tgccgaggtc gccggtgttc
     1141 agccagccgt cgtcgctcag gaccttggcg gtaagctcgg gccttttata gtagccgagc
     1201 atcacctgcg ggccgcgcac gtgcaccacg cccttgcggc acctgccgac gtcgatgccg
     1261 gcctcgtctg taatcctgaa ctcggtgccg cggatcgccg gtccgacggt tcccagcacc
     1321 ggcttgcgct gcgggcggag gcctataacc ggcgcagtct cggtcaggcc gtagccctcg
     1381 agtatctgaa cgcctatcgt atcgaagaag atgtccaccg aagcgggcat cgcgccaccg
     1441 ccggagacgc cggccttgaa gcgccctccg agcttggtct tgatcttttt gaagacgagg
     1501 atctcgccga ggccgcgta
//
LOCUS       DAOOLN010000550         1515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_108703_length_1515_cov_3.493836, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000550 DAOOLN010000000
VERSION     DAOOLN010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108703_length_1515_cov_3.493836"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="PLW80_07495"
     CDS             <1..535
                     /locus_tag="PLW80_07495"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPB66388.1"
                     /translation="GKVSSAPWAMVFPRAKRFPTSLPWVREVAEKAGLALDGAMVNLP
                     RHPSQLYEALFEGVVLWLVLWFIVRPRKPFKGFAVGAYLVGYGLVRFVIEYFREPDAD
                     LGYIVKLGDPDASIHLFTTPLNFSMGQLLCFMMIVGGLAFIAIVGRRAKRESSLLAAA
                     EAERARLDSAKKKLRKR"
     gene            complement(573..>1515)
                     /locus_tag="PLW80_07500"
     CDS             complement(573..>1515)
                     /locus_tag="PLW80_07500"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPB66389.1"
                     /translation="DLAPDDFSGILTRGGTILGSSREKPFKPSDDAGELKDSEVGHDK
                     PELILENYARLSLDALVVLGGNGTNTTAHLLAKQGLNVVGLPKTIDNDIWGTDVTFGF
                     HSAVDIATEAIDRLHSTAHSHNRVMVIELMGHKAGWLALYAGVAGGGDVILIPELPYD
                     PASVARDIEARRAEGKQFSIVVVAEGAMSREEAAMPKKERKRLREGMPYPSIGYRVAA
                     EIQAATGMETRVTVLGYLQRGGTPSPYDRVLATSFGTAAAELLARGRFGRMVAMRDSG
                     VTSVPLSEVADKTKTIPADHYMIGTARLVGTCFGDAR"
BASE COUNT          215 a          504 c          521 g          275 t
ORIGIN      
        1 gggcaaggtc agctccgcgc cgtgggcgat ggtcttcccg cgggccaagc gcttcccgac
       61 gagcctgccc tgggtccgtg aggtggccga gaaggcgggc ctggcgctcg atggcgcgat
      121 ggtcaacctg cctaggcacc cgtcgcagct ctacgaagcg ctgttcgagg gcgtcgtcct
      181 ctggctcgtg ctgtggttca tcgtgcgtcc gcgcaagccg ttcaagggct tcgccgtcgg
      241 cgcctacctg gtcggctacg gcctcgtccg cttcgtcatc gagtacttca gggagcccga
      301 cgccgacctc ggctacatcg tcaagctggg cgaccccgac gcgtccattc acctgttcac
      361 cacgccgctc aacttctcga tggggcagtt gctctgcttc atgatgatcg tgggcggact
      421 ggccttcatc gcgatagtcg gccggcgggc taagcgcgag tcgtcgctgc tggcggccgc
      481 cgaggcggag cgggccaggc tcgacagcgc caagaagaag ctcaggaagc gctgagcggc
      541 cgggcgggcg tgatagcttc ggccggcccg ccctagcgcg cgtcgccgaa gcaggttccg
      601 acgaggcgcg cggtgcctat catgtagtgg tcggccggga tcgtcttggt cttgtcggcc
      661 acctccgaca gcggcaccga ggtgactccg gagtcgcgca tcgcgaccat gcggccaaag
      721 cggccgcgcg cgagcagttc ggcggccgcg gttccgaagc tggtcgccaa cacgcggtcg
      781 taggggctcg gcgtgccgcc gcgctgcaga tacccgagta ccgtcacgcg ggtctccatg
      841 ccggtggccg cctggatctc cgcggccacc cgatagccga tgctcgggta gggcatgccc
      901 tcgcgtaggc gcttacgctc cttcttcggc atagccgcct cctccctgga catggctccc
      961 tccgcgacga cgacgatgga gaactgcttg ccttcggcgc gcctggcctc gatgtcgcgc
     1021 gccacggagg ccgggtcgta gggtagctcc ggtatcagga tcacatcgcc gcctccggcc
     1081 acgccggcgt acagggccag ccaaccggcc ttgtggccca tcagttcgat caccatcacc
     1141 ctattgtgcg agtgggccgt cgagtggagg cggtcgatgg cctccgtggc tatgtcgacg
     1201 gccgaatgga agccgaaggt cacatcggta ccccagatgt cgttgtctat ggtcttgggc
     1261 aggccgacca cgttgaggcc ctgcttggct agcaggtggg ccgtggtgtt cgtaccgttg
     1321 cctccgagca cgaccagcgc gtccaggctg aggcgggcgt agttctcgag aatcagctcg
     1381 ggcttgtcgt ggccaacctc tgagtccttg agctcgccag cgtcgtcgga aggcttgaac
     1441 ggcttctcgc gggacgaacc gagtatggtg ccgcctcgcg tcaggatgcc cgagaagtcg
     1501 tccggcgcca ggtct
//
LOCUS       DAOOLN010000551         1515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_108779_length_1515_cov_1.476712, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000551 DAOOLN010000000
VERSION     DAOOLN010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108779_length_1515_cov_1.476712"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(361..1203)
                     /locus_tag="PLW80_07505"
     CDS             complement(361..1203)
                     /locus_tag="PLW80_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPB66390.1"
                     /translation="MVIALAAASALFYGLGDFSGGYAAGKSKVLSVLVVSQACGLATA
                     LLAVAVTQPAPPGSVDLVWGLLGGVAGAFGIVTLYRGIAKGVVAIVSPTAALVSAALP
                     IAVGLALGDEPGSLAFVGMALCVPAVVLLSLGSGGGASGRGGTTASLIHGLVAGLGFG
                     LFYVAMSRPGVGSGFWPLVAARVASIAAALAVMLARREPLVVNRGGRLAAIFAGVLDM
                     AANILFVLASSAGLLSIVAVIVSLYPAPTVLMARLVFKERIGPVRILGLLLTLAGLAL
                     ISLG"
     gene            1305..>1515
                     /locus_tag="PLW80_07510"
     CDS             1305..>1515
                     /locus_tag="PLW80_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPB66391.1"
                     /translation="MIDYRLLEAFAAVLDLGGFERAADGLGLTQSAVSQRVKALEDEM
                     GRMLVVRDTPPRPTPAGERILRHYRQ"
BASE COUNT          300 a          484 c          496 g          235 t
ORIGIN      
        1 aacaaggatt cgacgcgcat catgcctccg ctaccgtccc gacccagctc gggaaacatg
       61 gcgcgaagta tatatgataa gtgcaagcaa tatctaggtt cacgacgcgc cgccccaggc
      121 aggattatga atgtattcag gcctcgtata tcatctcgga gggtcctgcc aaagagacgg
      181 gaccctcgtt aacgcgaggg ccgccgtctc tttggcaccc tcctccatgg aaatgcgagg
      241 cctggttacg aacagccttc tcggggcggc gcgtcgtgcc gtgcatcgag tggctatcgg
      301 acgccggtaa tcagctcggt ttgggcggcg cgtcgaaccg ggtcggaaga cggcgcgcgc
      361 tcatcccagg cttatcagcg ccagccccgc cagcgtcaat aggagcccca ggatcctgac
      421 cgggcctatg cgttccttga aaacgaggcg ggccatcagc acggtaggcg ccggatagag
      481 cgatacgatg acggcgacta tggagagcaa gcccgcgctg gaggctagca cgaacagtat
      541 gttggccgcc atgtccagga cgccggcgaa tatggccgcg agccgccctc cccggttcac
      601 gacgagcggt tcccgcctgg cgagcatgac ggcgagggcc gcggcgatgg aggcgacgcg
      661 agccgcgacg aggggccaga agcccgagcc gacgcccggt cgcgacatcg ccacgtagaa
      721 taagccgaat ccgaggcccg cgaccaggcc gtggatcagc gaggccgtgg ttccgccgcg
      781 gcccgatgcc cctcccccgg agccgagcga gagtaggacg acggccggta cgcatagcgc
      841 catgcctacg aaggctagcg atcccggctc gtcgccgagg gcgaggccga cggcgatcgg
      901 cagcgcggcg ctcacgaggg cggcggtcgg agagacgatg gcgacgactc ccttggcgat
      961 gccgcgatat agggtgacga taccgaaggc gccggccacg cctcccagta ggccccagac
     1021 caggtcgacg gatcctggcg gcgccggctg agtgacggcg acggccagca gggccgtagc
     1081 caatccgcag gcctgggaca cgaccaatac cgacaagacc ttgctctttc cggcggcgta
     1141 tccgccggag aagtcgccga ggccgtagaa cagggccgac gccgcggcta aggctataac
     1201 cattcattgg ctccgaacgt gagtttcgtc gtcagcatac tggaaccatg aatattagtg
     1261 aaattaatta tttgaatgct gcataaatcc tgctactatg ccatatgata gattatcgcc
     1321 tcctcgaggc cttcgcggcg gtgctcgacc ttggcggctt cgagcgagcg gccgacggcc
     1381 tcggtctcac ccaatcggcg gtgtctcagc gggtaaaggc gctggaggat gagatgggcc
     1441 gtatgctcgt cgtgcgcgac accccgcccc gccctacccc cgccggcgag cggatcctca
     1501 ggcattaccg ccagg
//
LOCUS       DAOOLN010000552         1514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_108830_length_1514_cov_2.932831, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000552 DAOOLN010000000
VERSION     DAOOLN010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108830_length_1514_cov_2.932831"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="PLW80_07515"
     CDS             <1..175
                     /locus_tag="PLW80_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPB66392.1"
                     /translation="VILVMLSAWGWLWGFAGLVLAVPMTVIIKIVCENVPGLEAVSIM
                     MGSYQEVKKKRGA"
     gene            complement(293..>1514)
                     /locus_tag="PLW80_07520"
     CDS             complement(293..>1514)
                     /locus_tag="PLW80_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HPB66393.1"
                     /translation="TTFMTMAYILAVNPGILSATGMPAGGVFTATALASMIATLLMAF
                     MANLPIALAPGMGLNAFFAFTVVLTMGYSWQLALTAVFVEGIIFILMSFFNIREAIVK
                     SIPQNIKNAVSVGIGLFIAFIGLQNAGIIANNDATLVSLGDVTKGAPLVAIIGLVVSG
                     VLLAFKVKGALLIGIVATTIIGIPFGVTSIPKGWSPVSAPAAPLFMQFEFSQIFTLDF
                     VVVMFTFLFVDIFDTIGTLVGVTTQAGIISKNGEIPKVKQALLADAIGTVAGAALGTS
                     TVTSYVESASGVAEGGRTGLTSLTTGILFGLALFLSPLFLLVPSAATAPALILVGLFM
                     LSPVKDIDLSDFTEAIPAFLTIIMMPLAYSIAEGLVFGILSYIFLKAITGKAKDISIV
                     TWIVGAFFILKHLL"
BASE COUNT          317 a          407 c          537 g          253 t
ORIGIN      
        1 cgtcatactc gtgatgctgt cagcctgggg ctggctgtgg gggttcgcag gcctggtgct
       61 ggcggtgccg atgacggtca tcatcaagat agtctgcgag aacgtgcccg ggctcgaggc
      121 ggtatcgatc atgatgggct cgtaccaaga agtcaagaag aagcgcggcg cctagcgaac
      181 cacagaggcg caggggaaaa cgagagagca aggatggatc cttgctctct cgttttttta
      241 tccgtgtatc tcagtgcctc tgtggcaggt ttcctggcaa gtacgcctta ccttacagca
      301 ggtgcttcag gatgaagaac gcgccgacta tccaggtaac gatcgagatg tcctttgcct
      361 tgccggtgat cgccttcagg aagatgtagg acaggatacc gaagacgagg ccctcggcga
      421 tgctgtacgc cagcggcatc atgatgatgg tcaggaaggc cgggatcgcc tcggtgaagt
      481 ccgacagatc gatatccttg accgggctga gcatgaacag gccgacgagg atgagggccg
      541 gagcggtcgc ggcgctcggg acgagcagga acaagggcga caggaacagc gcgaggccga
      601 acaggatgcc ggtggtcagc gaggtgaggc cggtgcggcc gccctcggcg acgccggagg
      661 cgctctccac gtacgaggtg acggtcgagg tgccgagcgc ggcgccagcc acggtgccga
      721 tggcgtcggc gagcagggcc tgcttgacct tcgggatctc gccgttcttg cttataatac
      781 cggcctgggt agtcactccg accagggtgc cgatcgtatc gaagatatcg acgaacagga
      841 aggtgaacat gacgacgacg aagtcgagag tgaagatctg cgagaactcg aactgcatga
      901 agagcggggc ggccggcgcg ctgaccgggc tccagccctt cggtatcgag gtcacgccga
      961 acgggatgcc gatgatcgtg gtcgcgacga tgccgatgag cagggcgccc ttgaccttga
     1021 acgcgagcag cacgccggag acgacgaggc cgatgatggc gacgagcggg gcgcccttgg
     1081 tcacgtcgcc gagcgacacg agcgtagcgt cgttgttggc gatgatgccg gcattctgga
     1141 ggccgatgaa cgcgatgaac aggccgatac cgacggagac ggcgttcttg atgttctgcg
     1201 ggatcgactt gacgatcgcc tcgcggatat tgaagaacga catcaggatg aagatgatgc
     1261 cctcgacgaa taccgcggtc agcgcgagct gccacgagta gcccatcgtg aggacgacgg
     1321 tgaacgcgaa gaacgcgttc aggcccatgc cgggcgcgag ggcgatcggc aggttggcca
     1381 tgaaggccat cagcagggta gcgatcatcg acgcgagggc cgtcgccgtg aacacgccgc
     1441 cggccggcat gccggtagcg ctgaggatac cggggttgac cgccaggatg tacgccatag
     1501 tcatgaacgt ggtg
//
LOCUS       DAOOLN010000553         1513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_108918_length_1513_cov_3.323045, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000553 DAOOLN010000000
VERSION     DAOOLN010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108918_length_1513_cov_3.323045"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PLW80_07525"
     CDS             complement(<1..196)
                     /locus_tag="PLW80_07525"
                     /inference="COORDINATES: protein motif:HMM:NF015546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HPB66394.1"
                     /translation="MLEPAELHVSGTMRKLIKKRRFRLSLDRAFGEVIRACSASPRRG
                     QRGTWITSDMIDAYERMHALG"
     gene            complement(230..397)
                     /locus_tag="PLW80_07530"
     CDS             complement(230..397)
                     /locus_tag="PLW80_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66395.1"
                     /translation="MRKPRFPWLGEDDGFAFPPVSGASPDGVLCVGGNLSPGMLLSAY
                     RQGVFPWFNDD"
     gene            complement(409..>1513)
                     /locus_tag="PLW80_07535"
     CDS             complement(409..>1513)
                     /locus_tag="PLW80_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB66396.1"
                     /translation="EGASHEGASHEGASHEGATATAAGDAAGNEVNGAGATETLAIGR
                     GEIETVVAKIARIPEKSVSQTERDRLATLEADLKASVFGQDAAIEAVAKAVKRGRAGF
                     RAPDKPVANFLFVGPTGVGKTELARTLADTLGVALHRFDMSEYQEKHTVSRLIGSPPG
                     YVGYEEGGLLTDAIRKTPHAVVLLDEIEKAHPDVYNILLSIMDYATLTDNQGRKADFR
                     NVVLIMTSNAGARDIGKPMIGFGERAFTEAAIDEAVQKAFTPEFRNRLDAVVRFGKLP
                     REVIERIVVKALDDFRAQLADKKVILEATDACVKLLAELGYSDEFGARNVGRVVEERI
                     KSWFVDEVLFGALKDGGAARADVEDGRIVIKAL"
BASE COUNT          224 a          501 c          522 g          266 t
ORIGIN      
        1 agcccagggc gtgcatgcgc tcgtaggcgt cgatcatgtc cgaggtgatc caggtgccgc
       61 gctggccgcg cctgggcgag gctgagcagg cgcgtatcac ctcgccgaag gcccggtcga
      121 ggcttaaacg gaagcggcgc ttcttgatca gtttgcgcat ggtgccggag acgtggagtt
      181 cggccggctc gagcacgaag cgcgggtcgg ggctccacca gacgatgggc tagtcgtcgt
      241 tgaaccacgg gaagacgccc tggcggtagg cggatagcag catgcccggc gacaggttgc
      301 ccccgacgca gagcacgccg tccggcgacg cgccggatac tggcgggaag gcgaagccgt
      361 catcctcgcc gagccatgga aagcgcggct ttctcatctc gggggccgct agagcgcctt
      421 gatgacgatc cggccgtcct ctacgtcggc acgcgcggcg ccgccgtcct tgagagcgcc
      481 gaagagcacc tcgtcgacga accaggactt gatgcgttcc tcgacgacgc ggccgacgtt
      541 gcgggcgccg aattcgtccg aatacccgag ttccgccagg agcttgacgc aggcatcggt
      601 cgcctcgagg atcaccttct tgtcggcgag ctgggcgcgg aagtcgtcga gcgccttgac
      661 cacgatacgc tcgatcacct cgcgcggaag cttgccgaag cgcacgaccg cgtcgagccg
      721 gttgcggaac tccggggtaa aggccttctg tacggcctcg tcgatcgcgg cctcggtgaa
      781 cgcccgctcg ccgaagccta tcatcggttt gccgatgtcc cgagcgccgg cgttgctcgt
      841 catgatcagc acgacgttgc ggaaatccgc cttgcgaccc tggttgtcgg tcagcgtcgc
      901 gtagtccatg atggacagaa ggatattgta gacgtcgggg tgggccttct cgatctcgtc
      961 gagcaggacg accgcgtgcg gcgtcttgcg tatcgcgtcg gtcaggaggc cgccctcctc
     1021 gtagccgacg tagcccggcg gcgagcctat gagccggctc acggtgtgct tctcctggta
     1081 ttcgctcatg tcgaagcggt ggagggcgac gccgagggta tcggccagag tgcgcgccag
     1141 ttcggtcttg cccacgccgg tcgggccgac gaacaggaag ttggccacag gcttatccgg
     1201 cgcgcggaaa ccggcccggc cacgcttgac ggccttggct acggcttcga tggccgcatc
     1261 ctgcccgaag acgctcgcct tgagatccgc ctcgagggtg gcgagcctat cacgctcggt
     1321 ctgcgacacg ctcttctcgg ggatacgcgc tatcttggcg acgaccgtct cgatctcgcc
     1381 gcggccgatg gccagggtct cggtggcgcc cgccccgttc acctcgttcc cggccgcgtc
     1441 gccggcggcg gtcgcggtgg ctccctcgtg cgaggctccc tcgtgcgagg ctccctcgtg
     1501 cgaggctccc tcg
//
LOCUS       DAOOLN010000554         1512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_109030_length_1512_cov_3.136582, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000554 DAOOLN010000000
VERSION     DAOOLN010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109030_length_1512_cov_3.136582"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /locus_tag="PLW80_07540"
     CDS             <1..1319
                     /locus_tag="PLW80_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB66397.1"
                     /translation="EKPVPYFLSMLSYVTYLLVSGKFLAEAGAAFGRDQTRILYNGAY
                     IMTEFEPQVTRVLTKNKKYWDQKNVHIGRLIYRYNKEYATLAPELFLRGQTSYAAIPS
                     TILDTWMKDPAKKAQIHPARTSSYTYFYGFNFKPTFAAEYEPDNWKVAVNNKNFRKAV
                     FHAFDRKAAMLTAEPYAPSRRLQSTVTPRGFVSAGGLDYVATGSLLAIANGDSFDKAK
                     ALQYKAAAMAELAGKATFPVKVMMPYNSSGSEWTNRVQVVEQQLEALLGKDFIDVIPV
                     SFPPTGFLNATRRSGNYAMQELNWGPDYADPQTYTDPFVSGANYIFIDSSPVYAKGDG
                     STAYDELVRAAMAETGDLKKRYELFAKAEAFLIEEAMIIPYAMGGGGFEASKLEPFTC
                     PYAPFGMSNLKFKGQIVLDKALDTEAYERLEAQWKKEREAALKKAR"
BASE COUNT          315 a          506 c          453 g          238 t
ORIGIN      
        1 tcgagaagcc ggtaccgtac ttcctgtcga tgctcagcta cgtcacctac ctactggtca
       61 gcggcaagtt cctggcagag gccggcgccg ccttcggcag ggaccagacg aggatcctat
      121 acaacggcgc ctacatcatg accgagttcg agccccaggt gacgagggtg ctgaccaaga
      181 acaagaagta ctgggaccag aagaacgtcc atatcgggcg cctgatctat cgctacaata
      241 aggagtacgc gactctggcc cccgagctgt tcctgcgcgg gcagacctcg tacgcggcca
      301 tcccgtcgac catcctggac acctggatga aggacccggc gaagaaggcc cagatacacc
      361 cggctcggac cagctcctac acctacttct acggcttcaa cttcaagccg accttcgccg
      421 ccgagtacga gccggacaac tggaaggtcg ccgtcaacaa caagaatttc cgcaaggcgg
      481 tctttcacgc gttcgaccgc aaggccgcga tgctaaccgc cgagccatac gcgccgagcc
      541 gcaggctcca gtcgaccgtg acgccgcgcg gcttcgtgag cgccggcggg ctcgactacg
      601 tcgccaccgg atccctgctg gccatcgcca acggcgactc gttcgacaag gccaaggcgc
      661 tccagtacaa ggccgccgcg atggccgagc tggccggcaa ggccacgttc ccggtcaagg
      721 tgatgatgcc gtacaactcc agcggctccg agtggacgaa ccgcgtccag gtcgtcgagc
      781 agcagctcga ggccctgctc ggtaaggact tcatcgacgt gataccggtg tcattcccgc
      841 cgacgggctt cctcaacgcg actaggcgct ccggcaacta cgcgatgcag gagctcaact
      901 ggggcccgga ctacgccgat ccgcagacct acaccgaccc cttcgtctcc ggcgccaact
      961 acatcttcat cgatagctcg cccgtctacg ccaagggcga cggctcgacc gcctacgacg
     1021 aactcgtgcg ggccgcgatg gccgagactg gcgacctcaa gaagcgctac gagctattcg
     1081 ccaaggccga ggccttcctg atcgaggaag cgatgatcat accgtacgca atgggcggcg
     1141 gcggcttcga agcgtccaag ctcgagccct tcacctgccc ctacgcgccg ttcggcatgt
     1201 ccaacctgaa gttcaaggga cagatcgtgc tggacaaggc tctcgacacc gaggcctatg
     1261 agcggctcga ggcccagtgg aagaaggagc gcgaggcggc gctcaaaaaa gcgcgatagc
     1321 ggaccttcgg ccgctatcgc gtagggtaag accgtatcgc cgacgtagcg cggcgatacg
     1381 gcagccgttg ttcaagcggt cgagctagcg ctcgccgctt aacagcatgg agccgacctt
     1441 cggccgctat cgcgtaaggt aagaccgtat cgccgacgta gcgcggcgat acggcagccg
     1501 ttgttcaagc gg
//
LOCUS       DAOOLN010000555         1512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_109037_length_1512_cov_3.036376, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000555 DAOOLN010000000
VERSION     DAOOLN010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109037_length_1512_cov_3.036376"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PLW80_07545"
     CDS             complement(<1..771)
                     /locus_tag="PLW80_07545"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB66398.1"
                     /translation="MIRLLEARGLSAGYGERVVVADASFSVSAGSLLVALGPNGCGKT
                     TVLKTCLGLLPPLSGTALLGGRPVDALRPRERARLAAWVPQLAESAWSYRSRDIVAQG
                     RFAVRGPFMPLNEDDEAAVDAALDAMDARAFAERPFSSLSGGEARRVMIARALAQETP
                     LLVLDEPAAHLDPGRQMELMELLAGLARSGKAVVVSVHDVNAARRFSDLALLVGKDGS
                     AAFGSPEEVLTPDLLEDAYDTHFIHGDHESYGPYVLPLS"
     gene            complement(768..>1512)
                     /locus_tag="PLW80_07550"
     CDS             complement(768..>1512)
                     /locus_tag="PLW80_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPB66399.1"
                     /translation="YVIGSSGGAALGAVCAIAFGSGTGALGFSGIGLAAFAGGLGATL
                     IVYLVAGATRLRSDASSLLLAGTALGSLFSALVSIVLALKDKDLHQAWFWLLGGFSGR
                     DLPELAASAPAMLAGFAAALAGSRSLDLLSVGDEEATSLGLRPATARFVIGGFAALSV
                     SAAVALSGAIGFVGLVSPHIARRFVGPRHARLIPASALVGASLLVVSDVVARTAFAPV
                     EMPVGAMTALIGAPFFVYKIARRGLDGAR"
BASE COUNT          262 a          508 c          572 g          170 t
ORIGIN      
        1 cgacaggggg agcacgtagg gtccgtacga ctcgtggtcg ccgtgtatga agtgcgtatc
       61 gtaggcgtcc tcgagcagat ccggtgtcag gacctcttcg gggctcccga aggcggccga
      121 gccatccttg ccgacgagca gggccaggtc ggagaagcgc ctagcggcgt tgacgtcatg
      181 gacgctgacg acgacggcct tgcccgagcg cgcgagaccg gccaggagct ccatcagctc
      241 catctgccga cccggatcca gatgggccgc gggctcgtcg agcacgagga gcggcgtctc
      301 ctgcgccaag gccctggcga tcatcacgcg cctggcctcg ccgcccgata gcgacgagaa
      361 gggacgttcg gcgaaggctc gggcgtccat ggcgtccagg gccgcgtcga cggccgcctc
      421 gtcgtcctca ttcagcggca tgaacgggcc gcgcacggcg aagcgtccct gggcgacgat
      481 gtcgcgcgag cggtacgacc aggcggactc ggcgagctgc ggcacccagg ccgcgagcct
      541 cgcccgctcc ctggggcgca gggcgtcgac cggccgtcct ccgagcaacg ccgttccgga
      601 cagtggcggc aggagcccga ggcaggtctt gagcacggtg gtcttgccgc agccgttggg
      661 gcccagggcg acgagcagcg atccggccga taccgagaaa gatgcgtccg ctacgacgac
      721 ccgctcgccg taacccgccg agaggcctcg cgcctcgagc aagcgtatca tcgggctcca
      781 tccagtccgc gcctggctat cttgtatacg aagaacggtg cgcctatcag cgcggtcatc
      841 gccccgaccg gcatctcgac cggcgcgaag gccgtgcgag cgacgacgtc ggagacgacg
      901 aggagcgagg cgccgacgag ggccgaggcg ggaatcagcc tggcatgccg gggcccgacg
      961 aagcggcgcg cgatgtgagg cgagacgagc ccgacgaagc cgatggcgcc ggagagggcg
     1021 acggccgccg atacggacaa cgccgcgaag ccgccgatga cgaagcgggc agtggccggc
     1081 ctgaggccca ggctggtcgc ttcctcgtcg ccgaccgata ggaggtcgag ggagcgtgaa
     1141 ccggccagcg cggccgcgaa gccggccagc atcgccggcg cggacgccgc gagctccggc
     1201 aagtcccgcc cggagaagcc gccgagcagc cagaaccagg cctggtgcag gtccttgtcc
     1261 ttgagcgcga gcacgatcga tacgagggcg gagaacagcg agccgagggc ggtgccggcc
     1321 aggagcaggc tcgaggcgtc ggagcggagg cgggtagcgc cggcgacgag gtagacgatg
     1381 agggtggcgc cgagcccgcc agcgaaggcc gcgagtccga tgcccgagaa gccgagcgct
     1441 ccggtccccg agccgaaggc tatcgcgcag accgcgccga gggcggcgcc gccggagcta
     1501 ccgatgacgt ag
//
LOCUS       DAOOLN010000556         1509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_109414_length_1509_cov_2.784044, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000556 DAOOLN010000000
VERSION     DAOOLN010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109414_length_1509_cov_2.784044"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /locus_tag="PLW80_07555"
     CDS             complement(<1..1459)
                     /locus_tag="PLW80_07555"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPB66400.1"
                     /translation="MITQQDIDEAEVRLRPVLGVAPRLYVAWLYGLGLLAAAFMLLLY
                     PGLRHPGATYSFTVDPPGSAVFIDGAYVGYTPCELFVPAGEHELRVSRPGFADHSASM
                     TTKGRVFGTLIVKPREELFVSLEGADVEAVLSGGIERYASWALAGTPSESYQIPMDLS
                     DAVRAAGVVGDRADGLLGAAASYARHSQSLRDAARAASIAYGGSAAVTPASAGRLVAA
                     VIGELRDDPAILTVLAALGPETVRRRLESTGAYRAALGASDAVVEPVAGRSSMFAGEE
                     FVPMGGGVSIVKAGSALPAVSQLRPFWLASSETTVGRYRRFVAERPEWAPSAAASLAA
                     EGLADDAYLTGFGEAADSDPIRYVSKPAAEAYCAWLSESAPAGYRFALPTEAQWSYAS
                     AASGASASVGAALLDDGRDAPLAPALLRYDAAGLRGMLGNVWEWCADSYAPHPAAGIA
                     GRLRFPSPEAVVRGGSWANRADLVTLSSRGPVRTVD"
BASE COUNT          235 a          512 c          571 g          191 t
ORIGIN      
        1 agtcgaccgt cctgaccggc ccgcgggacg acagggtgac gaggtcggcc cggttggccc
       61 aggatccgcc cctgacgacg gcctcgggcg acgggaagcg cagccggccg gctatcccgg
      121 cggctggatg cggggcgtac gagtcggcgc accattccca gacgttcccg agcatgcccc
      181 tgagcccggc tgcgtcgtag cgcagcagcg ccggcgcgag aggcgcgtct cgtccgtcgt
      241 cgagcagcgc ggcgcccacg gaggcgctgg cgcccgaggc ggcggacgcg tacgaccact
      301 gagcctcggt cggcagcgcg aagcggtagc cggccggcgc ggactcggac agccaggcgc
      361 agtaggcctc ggcggccggc ttcgacacgt atcggatcgg gtcggagtcg gcggcctcgc
      421 cgaaaccggt aaggtacgcg tcgtccgcga ggccttcggc ggccaaggac gcggccgccg
      481 aaggcgccca ttccggccgc tccgctacga agcgcctata acgcccgacg gtcgtctcgg
      541 acgaagcgag ccagaagggc cttagctggc tgacggcggg aagggccgag ccggccttaa
      601 cgatcgaaac gccgccgccc atggggacga attcctcgcc ggcgaacatc gacgagcgcc
      661 cggcgaccgg ttcgacgacg gcgtctgacg ctccgagcgc ggctcggtag gcgccggtcg
      721 actcgagccg ccgcctgacc gtctcgggcc cgagcgcggc gagcacggtc aggatcgcgg
      781 ggtcgtcgcg cagctcgccg atcacggccg cgacgagcct gccggcgctg gccggcgtga
      841 ccgcggcgga tccgccgtag gcgatggagg cggcgcgggc cgcgtctcgt aacgactggg
      901 agtgcctggc gtacgaggcg gcggcgccga ggaggccgtc ggcccggtcg ccgacgacgc
      961 cggcggcgcg gacggcgtcg gacaggtcca tgggtatctg gtacgattcg cttggggtgc
     1021 cggccaaggc ccaagacgcg tagcgctcga tcccgccgga gaggacggcc tcgacgtcgg
     1081 cgccttccag cgagacgaac agctcttcgc gcggcttgac gatcagcgtg ccgaagaccc
     1141 ggcccttcgt cgtcatcgag gccgagtggt cggcgaagcc cggccgcgag acgcggagct
     1201 cgtgctcgcc ggccggcacg aacagctcgc acggcgtata gccgacgtag gctccgtcta
     1261 tgaacacggc ggagccggga ggatcgacgg tgaacgagta ggtagcccct ggatgccgga
     1321 ggcccgggta cagcaggagc atgaaggccg ccgccaggag gccgaggccg tagagccagg
     1381 cgacgtatag cctcggagcc acgccgagaa ctggcctgag ccgcacctcg gcctcgtcta
     1441 tatcctgttg ggttatcata gtatcgtcgt tcatcgcggg attatatggg cgcgcgccga
     1501 ggcttgtca
//
LOCUS       DAOOLN010000557         1507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_109619_length_1507_cov_3.230028, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000557 DAOOLN010000000
VERSION     DAOOLN010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109619_length_1507_cov_3.230028"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1507
                     /locus_tag="PLW80_07560"
     CDS             <1..>1507
                     /locus_tag="PLW80_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPB66401.1"
                     /translation="AIAAPGRAYPVDVGYRPPKPDERVEDAVARAIVEALESTDGDVL
                     AFLPGLREIEAVRASLSRRSDGSAARARRGCAPDILTLHGSMPLVEQRRVTLPPEGAS
                     RRVILATNVAETSLTVPRITAVVDSGLSRLSRFHAPSGLNRLVTERASLAETDQRKGR
                     AGRLGPGLCLRCWDGADILAPSRGPELARTELSAVALECAVRGSSRLEATRWLDEPPR
                     HMWDAAVETLGRIGLLDSGGRATESGRTAAALGAEPRAAAALLAAAAGDRPDDTHAAA
                     LATAALTERGDRDDDGDYRSGLERLLEAARRGRVDDRGARILREAGRLLSRLPGGPAF
                     SAERASRALESVGELLAPGFPDRLARRLADDSWEFPSGRRARSAYAPPRVGWLLALDA
                     DAGDPLGRIRRAAPVGERAARAAMERGSSVSIELEWRGLSASAWERRRFGVFALTERR
                     LERAPRERLEAGFAERLRVEGLAWLPWNDEARSIVDRARYVAARAGAAYGV"
BASE COUNT          185 a          558 c          577 g          187 t
ORIGIN      
        1 cggccatcgc ggccccaggc cgcgcctacc ccgtcgacgt cggataccgc ccgccgaagc
       61 ccgacgagcg cgtcgaggac gcggtggccc gggctatcgt cgaggcgctc gagtcgaccg
      121 acggcgacgt gctcgccttc ctgcccggcc tccgtgagat cgaggccgtg cgcgcctcgc
      181 tcagccgccg gtcggacggc tcggccgctc gggcccgccg aggctgcgcg ccggatatcc
      241 tgacgctcca cggctcgatg ccgctcgtcg agcagcgtcg ggtaacgctg cctccggagg
      301 gagcgagccg gcgggtcatc ctggcgacga acgtggctga gacgagcctt acggtgccga
      361 ggatcacggc ggtcgtcgac tccggcctat ccaggctgtc gcgcttccac gccccgagcg
      421 gcctcaaccg gctcgtgacc gagcgcgcgt ccctggcgga aaccgaccag cgcaagggcc
      481 gcgccggccg cctcgggccc ggcctctgcc tgcgctgctg ggacggggct gacatcctcg
      541 cgccgtcacg gggaccggag ctggccagga cggaactatc cgccgtagcg ctcgaatgcg
      601 cggtccgcgg atcttcgcgc ctggaagcga cgcgctggct cgatgagccg ccgcggcata
      661 tgtgggacgc cgccgtcgag acgcttgggc gcatcggcct cctcgactcg ggcggccggg
      721 ccacggagtc aggcaggacg gccgcggcgc tcggcgccga gccccgggcc gccgccgcgc
      781 tgctagcggc ggcggccggc gatcgccccg acgacacgca cgcggcggcc ctggccacgg
      841 ccgcgctgac cgagcgcggc gaccgggacg atgacggcga ctacaggagc ggcctcgagc
      901 gcctgctcga ggcggccagg cgcggccggg tcgacgaccg cggcgcgagg atactgaggg
      961 aggccgggcg cctcctgtcg aggctgcctg gcggaccggc tttcagcgcc gaacgcgcat
     1021 cgcgggccct ggaatccgtg ggcgagctcc tggcgcccgg cttccctgac cgcctggcgc
     1081 ggcgcctggc cgacgacagc tgggagttcc cgtccggacg gcgggccaga tccgcctacg
     1141 ctccgccgag ggtcggctgg ctcctggcct tggacgccga tgccggagac ccgctcggcc
     1201 ggatacgccg agccgcgccg gtgggggaac gggcggccag ggccgcgatg gaacgaggct
     1261 cgtcggtatc gatcgagctc gaatggcgcg ggctttcggc ctcggcctgg gagcggcgcc
     1321 gcttcggcgt cttcgcgctg accgagcgcc gcctcgagcg cgcgcccagg gagcggctag
     1381 aagccggctt cgccgagcgt ctgcgcgtgg agggcctagc ctggttgccg tggaacgacg
     1441 aggcgcgatc catcgtcgac cgagcgcgct acgtcgccgc cagggccggt gccgcttacg
     1501 gcgtgga
//
LOCUS       DAOOLN010000558         1505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_109886_length_1505_cov_2.378621, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000558 DAOOLN010000000
VERSION     DAOOLN010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109886_length_1505_cov_2.378621"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLW80_07565"
     CDS             <1..298
                     /locus_tag="PLW80_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66402.1"
                     /translation="ESVAFIGCSGGVYYDADSKRAYTAVDASGVPVDADGKALAKPKA
                     AGCIVASFSLSAMDGYATPASAAMPAGKALTGDAAVDIVGKARSEPWTIGAYEL"
     gene            344..1120
                     /locus_tag="PLW80_07570"
     CDS             344..1120
                     /locus_tag="PLW80_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66403.1"
                     /translation="MKTKETRSRVMDDPVLDRLFTEAYRDALARVGGLEQEKRLADHV
                     EAYAELFERDLDARDLSDSERREMVAAFKRRLASVREERDALRRRRRGRALIGGLIAA
                     CVLATGGLYLASAQPFKAAAEIERELGLCYAKVDAGTGAYAKRYFALLAKHGSKLGSM
                     KAEDYGRSMRAELDEHFNEYLDRVVAGELQYADDAKAWAKLFPDKEEREERYRAVDNA
                     FQAGLGKAVGATWDTVKKETGSLFEEAGEAVKKLVDTVER"
     gene            complement(1210..>1505)
                     /locus_tag="PLW80_07575"
     CDS             complement(1210..>1505)
                     /locus_tag="PLW80_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66404.1"
                     /translation="EQAIEAAGKASLAGWQGTAFDAYYVLNGIALFVISILMYRSDVY
                     TKATATWGLLAAIFMIIPSTAGTLGLVCSLASLVPWYVFGVRFAKVFRALGRP"
BASE COUNT          274 a          487 c          504 g          240 t
ORIGIN      
        1 cgagtccgtg gccttcattg gctgctcggg cggcgtctac tacgacgccg actcaaaacg
       61 cgcgtatacc gccgtcgatg cgtccggagt ccccgtggac gccgacggca aggccctggc
      121 caagcctaag gccgcgggct gcatcgtcgc gtcgttctcc ctgagcgcca tggatggcta
      181 cgcgacgccg gcctcggccg ccatgcccgc cggcaaggcc ctaacgggcg acgccgccgt
      241 cgacatcgtc ggcaaggccc ggagcgaacc ctggaccata ggagcctacg agctgtaggc
      301 cggccgcgtt ggcgcggcgg cgccttgtcg gtatagtagt gccatgaaga cgaaagagac
      361 caggtcacgg gtcatggacg atcccgtcct cgataggctc ttcacggagg cgtatcggga
      421 cgccctggcg cgggtaggcg gcctcgagca ggagaagcgc ctggccgacc acgtcgaagc
      481 gtacgccgaa cttttcgagc gtgatttgga cgcccgcgac ctatccgact cggagcggcg
      541 cgagatggta gccgccttca agcgtcgctt ggcttctgtt cgggaggagc gagacgccct
      601 tcgcagacgt aggcgcgggc gggccttgat cggtggcctt atagcggcct gcgtcctggc
      661 caccggaggc ctctacctgg cctcggccca gccgttcaag gcagcggcgg agatcgagcg
      721 cgagctcggt ctatgctacg ccaaggtcga cgccgggacc ggggcctacg ccaaacgcta
      781 cttcgcgctg ctcgctaagc atggctccaa gctaggctcg atgaaggccg aggactacgg
      841 acgctccatg cgcgcggagc tggacgagca tttcaacgag tacctcgacc gggtagtcgc
      901 gggcgagctc cagtacgccg acgacgccaa ggcctgggcc aagctcttcc ccgataaaga
      961 ggagcgcgag gaacgctatc gcgccgtgga caacgccttc caggccggcc taggcaaggc
     1021 cgtcggcgct acctgggaca ccgtcaagaa ggagaccggc tccttgttcg aggaggccgg
     1081 cgaagcggtc aagaagctcg tggatacggt cgagcgatga tggtatcggc ccgctaccga
     1141 agttgccggt tttacccgct tcctctttct cggccgagct tttcggcgcg ggccgagctc
     1201 ggcgtagccc tagggccttc ctagcgcacg gaagaccttg gcgaagcgaa cgccgaagac
     1261 gtaccaaggg acgagcgagg ccaacgagca taccaagccc agggtgccag cggtcgaggg
     1321 gatgatcatg aagattgccg cgagcaggcc ccaggtcgcg gtagccttgg tatagacatc
     1381 gctcctgtac atcaggatcg atatgacgaa cagggctatt ccgttgagca cgtagtacgc
     1441 gtcgaacgcc gtcccttgcc agcccgccaa ggacgccttg ccagccgcct cgatcgcctg
     1501 ctcgg
//
LOCUS       DAOOLN010000559         1504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_109943_length_1504_cov_6.685990, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000559 DAOOLN010000000
VERSION     DAOOLN010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109943_length_1504_cov_6.685990"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..1446
                     /locus_tag="PLW80_07580"
     CDS             244..1446
                     /locus_tag="PLW80_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HPB66405.1"
                     /translation="MSEERSQGRIISIDEGLVKSQLGDVVRETVEQTLNKMLDAEADE
                     LCGAKRYVRSPDRIDTRAGHYERTLDTKAGRVKLKVPKLRTIPFESAIIDRYRRRESS
                     VEEALMEMYLAGVSVRRVEDITEALWGTRVSAGVVSKLNQELYDKIDLWRTRPIEGTF
                     PYVYLDGIVLKRTWADEIRNVSILVAFGVNADGYREILGAQEGAKEDKAGWGSFVESL
                     KQRGLSGVQLFISDKCMGLVESLGEYYPDVPWQRCAVHFYRNVFTNVPSTKVNEVAAM
                     LKAIHAQEDRDASIQKAELVIEKLKAMKLPVAAKTVEDGIRETLTYTAFPREHWRSIR
                     TNNPMERIMREIRRRTRVVGNFPDGRSALMLVTARLRYIAGKEWGSRRYMDMSRLNSL
                     VRFEVLTG"
BASE COUNT          375 a          401 c          427 g          301 t
ORIGIN      
        1 aatttcatct tcaactgcag attttgatat tgtttctata cgcttaaatc gatcatcgtt
       61 agtagcgtga taattataga gcttcgtctc cattcgcatt aatcgttcca aatcggaatt
      121 ctttgatttc cttatacaaa tcaggtagcg tacaatatct ttcgcacatt tagcggtcat
      181 tgaaaaaggc gtcctcatct ggtttagttg agttaccaca caccaacgac caaggaggac
      241 gccatgagcg aagaacgctc acagggaagg attataagca tcgacgaagg gctcgtgaag
      301 agccaactcg gggatgtcgt cagggagacc gtcgagcaga cgctcaacaa gatgcttgac
      361 gcggaagccg acgagctatg cggcgccaag cgatacgtac ggagcccgga tcggatagat
      421 accagggccg gccactacga acgaacactc gataccaagg caggtagagt caaactcaag
      481 gtacccaaat tgagaacgat accattcgag agcgccatca tcgaccgcta ccgaaggcgt
      541 gagagctccg tcgaggaagc gctcatggag atgtacctcg caggcgtatc cgtgcgtcgc
      601 gtcgaagaca tcaccgaagc gctctggggc acccgcgtct cggccggcgt cgtatcgaag
      661 cttaaccagg agctgtacga caagatcgac ctgtggcgca cccgccctat cgaaggcacg
      721 ttcccgtatg tgtacctcga cggcatcgta ctcaagcgta cctgggcgga cgagatccgt
      781 aacgtatcga tcctagtcgc cttcggcgta aatgccgacg gatacaggga aatcctcgga
      841 gcccaggaag gggccaagga ggataaggcc ggctggggat cgttcgtcga atctctcaag
      901 cagcgaggcc ttagcggcgt tcagctattc atctccgaca agtgcatggg cctcgtcgag
      961 tcgctcggtg agtactaccc cgatgtcccg tggcagcgat gcgccgtgca tttctatcgg
     1021 aacgtgttta ccaacgtccc ctcgacgaag gtcaacgaag tggcggccat gctcaaggcg
     1081 atccatgcgc aggaagaccg tgacgcgtct attcagaaag ccgagctggt catcgagaaa
     1141 ctcaaggcca tgaagctccc tgtcgccgct aagaccgttg aagacggcat acgggagacc
     1201 ctaacgtata cggccttccc acgggagcac tggcgaagca tacggaccaa caacccgatg
     1261 gagcgaatca tgcgcgagat caggagaagg acgcgggtcg tcgggaactt cccggatggt
     1321 cgatctgccc tgatgcttgt aactgcgcgg cttcggtata tcgccggaaa ggaatggggc
     1381 tcgcgtagat acatggatat gtcgaggctg aatagcctcg tacgtttcga ggtactgacc
     1441 gggtagctac aagctactcc gggtgaggcg ccccgcaaat gtgcgaaaaa tggttgacgt
     1501 tatc
//
LOCUS       DAOOLN010000560         1504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_109977_length_1504_cov_3.509317, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000560 DAOOLN010000000
VERSION     DAOOLN010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109977_length_1504_cov_3.509317"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PLW80_07585"
     CDS             complement(<1..438)
                     /locus_tag="PLW80_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66406.1"
                     /translation="MPLGTIETVASIALAMIPPGNVAKELCGVRRAFWTELGAASARA
                     FFDYPVLSWLARPVDGAALAAAAARSRLPFELVGLERFGDDVFIRIPEEYAAEARGLA
                     SAFSEAGADTEYRPGPFQAGHGSYCATLAGVGERAAPIVELMPV"
     gene            487..1257
                     /locus_tag="PLW80_07590"
     CDS             487..1257
                     /locus_tag="PLW80_07590"
                     /inference="COORDINATES: protein motif:HMM:NF024626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HPB66407.1"
                     /translation="MLRKIEFLTERITAVLASWPSVDSVLSMNPSETDAIDPYFALVF
                     DVYYDGEIPSSDERQAMYDNPGAFESSAVVEKDRFFLDGLPIRIEFKKVKILEDFVRN
                     GYDARWAFGTVGTYVIYRVVNGKILFKRTDRLERVRAALKNCPDAFWLRIREAQQSKM
                     EHCLSDLGGAAMKDDRYFYFISLAGFMRACVSALFAINRRFEPSDRQMADSLAELPVL
                     PVDFSGRWSNLLRTDDSVYPQKRYQIAQLITRDVLALE"
     gene            1250..>1504
                     /locus_tag="PLW80_07595"
     CDS             1250..>1504
                     /locus_tag="PLW80_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66408.1"
                     /translation="MSRLLRGTRLVIGLAAAAAVIPCSCVAGYVDGLSFFDSDGGASR
                     VASEADGGSSVPAGATAIYRPESGYRAGAIEDLAVFVRGSG"
BASE COUNT          266 a          487 c          477 g          274 t
ORIGIN      
        1 gacaggcatc agctcgacga tgggcgcggc acgctcgccg acgccagcaa gggtcgcgca
       61 ataggaaccg tgcccggcct ggaacggccc ggggcggtac tccgtatcgg cgcccgcctc
      121 ggagaaagcg gacgccagcc cgcgggcctc ggccgcgtat tcctccggaa tacgtatgaa
      181 gacgtcgtcc ccgaatcgtt cgagtccgac cagctcgaac ggcaggcggc tcctcgcggc
      241 cgccgcggcg agagccgcgc cgtcgaccgg cctggccagc cacgatagca ccggatagtc
      301 gaaaaacgcc cgggcgctgg ccgcgccgag ctcggtccag aacgccctgc gaacgccgca
      361 tagctccttg gccacgttgc ctggcggtat catcgcgagc gctatggaag cgacggtttc
      421 tattgttccc aaaggcatga aggcatagta tactcggtct atcctcctgt aaaggaaagt
      481 tcggccatgc ttagaaagat agagttcctg accgagagga tcaccgcggt tctggcctcc
      541 tggccgagcg ttgacagcgt cctctcgatg aacccgtcgg agaccgacgc gatcgacccg
      601 tacttcgccc tcgtcttcga cgtgtattac gacggcgaaa taccgtcgag cgacgagcgc
      661 caagccatgt acgacaaccc gggagccttc gagtcgtccg cggtggtgga gaaggatcgc
      721 ttcttcctcg acggcctgcc gatacgcatc gagttcaaga aggtcaagat actcgaggat
      781 ttcgtgcgta acggctacga cgcccgctgg gccttcggca ccgtcggcac ctacgtcata
      841 taccgcgtcg tcaacggcaa gatcctgttc aagcggaccg accgcctcga gcgcgtacgg
      901 gcggcgctga agaattgccc ggatgcattc tggctcagga tccgcgaggc tcagcagtcc
      961 aagatggagc attgcctgag cgacctgggc ggggccgcca tgaaggacga ccgctacttc
     1021 tattttatat ctctggcagg cttcatgcgc gcctgcgtgt ccgccctctt cgccatcaac
     1081 cggcgcttcg agccgtcgga caggcagatg gccgattccc tggctgagct gcccgtgttg
     1141 ccggtggact tttccgggcg atggtcgaat ctgctccgta ccgacgattc ggtctatccg
     1201 cagaagcgct accagattgc gcagctcatc acgcgtgacg tgttagccct tgagtaggct
     1261 cctccgcgga acccgcctcg taatcggtct cgctgcggcc gcggctgtca taccctgctc
     1321 ctgcgtcgcc ggttacgtcg atggactgag cttcttcgac tcggacggcg gggcgtcgcg
     1381 cgtcgcgagc gaagcggacg gcggctcgag cgtcccggcg ggagcgacgg ccatctatcg
     1441 gccggaatcg gggtataggg ccggggctat cgaggatcta gccgtcttcg tacgcggttc
     1501 cggc
//
LOCUS       DAOOLN010000561         1504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_109995_length_1504_cov_3.242236, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000561 DAOOLN010000000
VERSION     DAOOLN010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109995_length_1504_cov_3.242236"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PLW80_07600"
     CDS             complement(<1..747)
                     /locus_tag="PLW80_07600"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HPB66409.1"
                     /translation="MRTLARWLAAALALASATPIAAQPAPLGAAPAIAPAASSPSSSP
                     ASSPYKVTLITILPGKALYSSFGHSGLRVVDARSGRDLFYNYGLSAHPFDAKFALGMF
                     AGRMEFMVGAGATSSVLGFYSGVENRTIIEQDLALDATQAAALVAALRRDARPENRIY
                     NYRYFSDNCTTRVAVMLQRTAGEDPGSRPVDAALTVRASVNGTLAGRPWLIFVINLLM
                     GPTVDRQAGTGDPIFLPGQLMDWAAEAVNGG"
     gene            complement(775..>1504)
                     /locus_tag="PLW80_07605"
     CDS             complement(775..>1504)
                     /locus_tag="PLW80_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPB66410.1"
                     /translation="SVTANIERMGSYYDKLLGKSDAGRGAIETVVAQVREIDSQSENL
                     QAANQLIAGIAAETNLLAMNAAIEAAHAGEAGMGFAVVADEIRKLAENAAVQSKTIGQ
                     TIKGIRGVIEAVVASSGQSASTFEEILEQIRILSRLEEEVKYSMQEQSAGSSQILESL
                     ASINEVTADVRHSAQEMQEGSNAVLTEMRQLLQLASELENGMSEMASGAEEIRRAAQD
                     TNELTIAASASVKNASAEVAQFKT"
BASE COUNT          224 a          492 c          518 g          270 t
ORIGIN      
        1 tccgccgttg accgcctcgg ccgcccagtc catcagctgg ccgggcagga agatcgggtc
       61 gccggtcccg gcctggcgat ccaccgtcgg gcccatgagc aggttgatga cgaagatcag
      121 ccagggcctg ccggcgagcg tgccgttgac cgaggcccgg actgtcagcg ccgcgtcgac
      181 cggcctggag ccgggatcct cgccggccgt cctctgaagc atcaccgcga cgcgggtcgt
      241 gcagttgtcg ctgaagtagc ggtagttata tatgcggttc tcgggcctgg cgtcgcgcct
      301 gagcgcggcc accagcgcag ccgcctgcgt cgcgtcgagg gccaggtcct gctcgatgat
      361 ggtacggttc tcgacgccgc tatagaagcc gagcacgctc gaggtggcac cggccccgac
      421 catgaactcc atgcgccccg cgaacatccc gagcgcgaac ttggcgtcga aagggtgggc
      481 cgagaggccg tagttataga acaggtcccg cccgctacgc gcgtcgacga cgcggagccc
      541 cgaatgcccg aaggacgagt agagggcctt gccgggcagg atggtgatca gggttacctt
      601 gtacggcgac gaggcgggcg acgaggaggg cgacgaagct gccggcgcta tggccggcgc
      661 tgcgcctagt ggagcgggct gggccgcgat cggcgtggcc gaggccagcg cgagcgccgc
      721 cgcgagccat cgagctaggg ttctcatggg aggagtatac aacggcgaag cctatcaggt
      781 cttgaactgg gcgacctctg cggaggcgtt cttgacgctg gccgacgcgg ctatcgtcag
      841 ttcgttcgtg tcctgggcag cgcggcgtat ctcctcggcc ccggaggcca tctcgctcat
      901 gccgttctca agctcgctgg ccagctgtaa aagctggcgc atctcggtca gcaccgcgtt
      961 tgagccttcc tgcatctcct gggccgagtg ccttacgtcg gcggtcacct cgttgatgct
     1021 ggccaaggac tcgagtattt gagacgagcc cgcgctctgc tcctgcatcg agtacttgac
     1081 ctcctcctcg agcctcgaga gtatccggat ctgctccagg atctcctcga aggtcgaagc
     1141 gctctggccg gagctggcga ccacggcctc gatgacgccg cggatgccct tgatggtctg
     1201 gccgatggtc ttggactgca cggcggcgtt ctcggcgagc ttgcgtatct cgtcggcgac
     1261 gaccgcgaag cccatgcccg cctcgccggc gtgcgccgcc tcgatggcgg cgttcatggc
     1321 gagcaggttc gtctcggccg cgatgccggc tatcagctgg tttgccgcct gcaggttctc
     1381 cgactgcgag tcgatctcgc ggacctgcgc gacgaccgtc tcgatggcgc cgcggccggc
     1441 gtcggatttt ccgagcagct tgtcatagta cgagcccatg cgctcgatgt tcgcggtgac
     1501 cgac
//
LOCUS       DAOOLN010000562         1504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_110001_length_1504_cov_3.139406, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000562 DAOOLN010000000
VERSION     DAOOLN010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110001_length_1504_cov_3.139406"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..>1504)
                     /gene="yqeB"
                     /locus_tag="PLW80_07610"
     CDS             complement(185..>1504)
                     /gene="yqeB"
                     /locus_tag="PLW80_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HPB66411.1"
                     /translation="VEVFGSRPRLVIVGAGHVGQAIARAAKAIGYRVAMVDDRPDYAT
                     AERLSCADELYVDAELAAAMAAAPIGALDCVVVSTPSHRSDEQAIRHYAGSDCRYLGV
                     LGSRRKVTAMFSRMRADGVPEERIAEVRAPIGLDLGAETPEEIAVSVLAEVMAVVSGR
                     DGAPLAGRQSDLIVVRGAGDLATGVIVRLKAAGFRVAALEIERPTAIRRSVALCEAVY
                     SGEARVEGVLARRVAGLEEARSAIADGVVPILVDPDCRYVRALAPVALVDATLSKRNT
                     GVRRGMAPAVIGLGPGFEAGGDVDAVIETQRGHDLGRVILRGKAADDTGVPGELGGKS
                     AERVIKSTAAGVVEAIASIGQMVEIGDPVIGVRGPDGLTVVKTTIAGVVRGAIRPGLE
                     VPVGMKIADVDPRGRVDSCHSVSDKARSIGGAVLEAILALKRRQKRD"
BASE COUNT          223 a          568 c          492 g          221 t
ORIGIN      
        1 ctggccaagg ctattgtcga cggtatcggt ttcgccggcg gccacgccca catgggcggc
       61 ggcttcatcc cggccacggc cctgccgccc ggcgagccag ccgaggcgct cgtcaagcgg
      121 cgcgccgtag cgtatatcga gggcctgggc ctctaaccgg gcctagccga gcctctaacc
      181 gggcctagtc gcgcttctgg cgcctcttga gcgcgagtat cgcctcgagg acggcgccgc
      241 ctatcgagcg ggccttatcc gagacgctgt ggcagctatc cacgcggccg cgcgggtcga
      301 cgtccgctat cttcataccg acgggcacct cgaggccggg ccggatcgcg ccgcgcacga
      361 cgccggctat cgtggtttta accacggtca gcccgtcggg cccacgcacg cctatcaccg
      421 ggtcgccgat ctcgaccatc tggcctatcg acgctatcgc ttcgacgacg ccggccgccg
      481 tagacttgat cacgcgctcg gcgctcttgc cgcccagctc gccgggcaca ccggtgtcgt
      541 cggccgcctt gccgcggagg atgaccctgc cgaggtcgtg gcctcgctgc gtctcgatca
      601 ccgcatcaac gtcgccgccc gcctcgaagc ccggcccgag tcctatcacc gctggcgcca
      661 tgccgcggcg gacgccagta ttgcgcttac tgagcgtcgc gtcgacgagg gcgacgggcg
      721 caagggcgcg gacgtagcgg cagtccgggt cgacgaggat aggcacgacg ccgtcggcga
      781 tcgcggaacg ggcctcctcg aggccggcga cccgcctcgc gagcacgccc tcgactcgcg
      841 cctcgcccga gtacacggcc tcgcacaggg cgaccgagcg cctgatagcc gtcggccgct
      901 ctatctcgag ggcggctacc ctgaaaccgg ccgccttgag cctgacaatg acgcccgtcg
      961 ccaagtcgcc ggcgcctcgc accacgatca gatcggactg acggcctgcc aacggagccc
     1021 cgtcgcggcc cgatacgacg gccatgacct cggccagcac cgatacggct atctcctccg
     1081 gcgtctcggc gcccaagtcg aggccgatag gagccctgac ctccgcgatt cgctcctcgg
     1141 gcacgccgtc cgcgcgcatg cgcgagaaca tcgcggtcac cttgcgcctc gagccgagca
     1201 ctccgagata acggcagtcg gacccggcat agtgtcgaat cgcctgctcg tcgctgcggt
     1261 gcgacggcgt cgagacaacg acgcaatcca gggcgccgat cggcgcggcc gccatcgccg
     1321 ccgcgagctc ggcgtcgacg tacagttcgt cggcgcacga taggcgttcg gccgtcgcgt
     1381 agtcgggccg gtcgtcgacc atagcgacgc ggtaaccgat ggccttggcg gccctggcga
     1441 ttgcctggcc gacgtgtccc gccccgacga tgacgagccg cggcctcgag ccgaaaacct
     1501 cgac
//
LOCUS       DAOOLN010000563         1502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_110216_length_1502_cov_2.708362, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000563 DAOOLN010000000
VERSION     DAOOLN010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110216_length_1502_cov_2.708362"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="PLW80_07615"
     CDS             <1..1156
                     /locus_tag="PLW80_07615"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HPB66412.1"
                     /translation="DALESASSDAATSAYWSAGWLRNAARFAAAARRQVRIRNGDGPI
                     VIACAGPSLTRFLPELSRRRDDIALWALASAVPALVAAGLTPDLAVATDPGFWNALHL
                     RTITELGIPIALSPSAFATEDALYRCPILALDTGLPFERAAVEAAGFEDHRCSASGSA
                     AGTALSLALACTGGPVTLAGYDLAAFGHEDHCRPYPFDSLDLALASRTRPVTSAGASR
                     TFDGYQAAVGAWRLSRAFSAYALSIRPPLGDSARAFRLSDSPVPTPVPRIARLDDLPP
                     TRGEPPVVTPLDDGSRDSSLDSAAIRREGYLDRVSSSLEASLAGATEALASSVPMPFD
                     AMTVYRALAPEATAPLVASMARGEATERELLAVHSAAEQALFSMRGGCDG"
     gene            1149..>1502
                     /locus_tag="PLW80_07620"
     CDS             1149..>1502
                     /locus_tag="PLW80_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66413.1"
                     /translation="MAEANLAHEPLDAQLFERNMLAVASCHGPVCADRIRSARSDPRV
                     GVQTSRSGHPVPFVGLRSRQALMHSRFDPVREAGRLASSYSKAAFYVFLGLGAGYEAA
                     AMLEADLVTRGLIVEY"
BASE COUNT          211 a          557 c          459 g          275 t
ORIGIN      
        1 tgacgcgctc gagtcagcca gctccgacgc cgcgacgagc gcgtactggt cagccggatg
       61 gctacgcaat gcggcccgtt tcgccgccgc cgcccggaga caggtccgta tacgcaacgg
      121 cgacggccct atcgtcatcg cctgcgccgg cccgagcctt acgcggttcc ttcccgagct
      181 tagccgtcgc cgcgacgata ttgccttgtg ggcccttgcc tcggccgttc cggcgctcgt
      241 cgccgccggc ctgacgcccg acctagccgt agccaccgat cccggtttct ggaacgccct
      301 gcaccttcgg accatcacgg aactcggaat cccgatcgcg ctgtccccga gcgccttcgc
      361 caccgaggac gccctctatc gatgccctat cctagcgctc gatactggct taccgttcga
      421 gcgtgcggcc gtcgaggctg caggtttcga agaccatcgc tgttcggcgt ccggtagcgc
      481 cgccggcacc gccttatcgc tcgcgctcgc atgtaccgga ggtccggtaa ccctcgccgg
      541 ctacgacctg gccgccttcg ggcatgagga ccattgcagg ccctacccct tcgactcgct
      601 cgacctagcg ctggcttcaa gaacgaggcc cgtaacgtcg gccggcgctt ccaggacgtt
      661 cgacggctat caggcggccg taggcgcttg gcgcctctct cgcgcattct ccgcctacgc
      721 cttgtcgatt cggccgccgc taggcgatag cgccagggcg ttcaggctct ccgactcccc
      781 cgtgccgact ccggtgcctc gcattgccag actcgacgac cttccgccga cgaggggtga
      841 gccgcccgtt gttacaccgc tcgacgatgg ctcgcgcgac tcgagtctgg actcggcggc
      901 cattcggcgt gaaggctacc tcgaccgcgt atcgtcgtcg ctcgaagcct cgctcgcagg
      961 cgcaaccgag gctctggcct cgtcggttcc gatgccgttc gatgccatga cggtatacag
     1021 ggccctcgct cccgaggcca ccgcgcccct cgtcgcctcg atggccaggg gcgaggcgac
     1081 agaacgcgaa ctcctcgccg tccactcggc ggccgagcaa gccctctttt cgatgcgcgg
     1141 aggctgcgat ggctgaggct aacctggctc acgagccgct cgacgcgcag ctcttcgagc
     1201 gcaatatgct ggccgtggcc tcgtgccacg ggccagtctg cgctgacagg atacgctcgg
     1261 cgcgatctga tccgcgcgtt ggcgtccaaa cctcccgttc ggggcatccg gtacccttcg
     1321 tcggcttacg ctcccgccaa gcccttatgc attccaggtt cgacccggtg cgagaggccg
     1381 ggcgactcgc ctcgagctac agcaaggcgg cgttctatgt cttcctcggg ctcggggcag
     1441 gctatgaggc cgccgccatg ctcgaggccg acctcgtgac caggggtctg atcgtcgaat
     1501 ac
//
LOCUS       DAOOLN010000564         1501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_110293_length_1501_cov_3.774550, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000564 DAOOLN010000000
VERSION     DAOOLN010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110293_length_1501_cov_3.774550"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..432
                     /gene="rplU"
                     /locus_tag="PLW80_07625"
     CDS             121..432
                     /gene="rplU"
                     /locus_tag="PLW80_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPB66414.1"
                     /translation="MYAIVEVQGKQYRAEKGAVLRVDRMDGEAGTPVAIDSVLLVSGD
                     TIKIGAPYVQGAQVKATIKAQEKGDKVIVFKYKPKKDYRRTQGHRQRYTLLTVEDIVG
                     A"
     gene            422..763
                     /locus_tag="PLW80_07630"
     CDS             422..763
                     /locus_tag="PLW80_07630"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HPB66415.1"
                     /translation="MGPDRLIVRLSFWPDGALKGARADGHAGDRRAGANLACAAVTVL
                     LRTAYETAAGYEGVTVRGIAPAPGSLSFEIGRYPPAMAERLRGVGDFLTVGLSSVDRE
                     YPGLIDFLLER"
     gene            776..1015
                     /gene="rpmA"
                     /locus_tag="PLW80_07635"
     CDS             776..1015
                     /gene="rpmA"
                     /locus_tag="PLW80_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPB66416.1"
                     /translation="MPHKGNGRDSNPQHLGVKAYGNELVRAGSIIVRQRGTKIHPGEN
                     VGKGKDDTLFAKADGRVVFCEHRGRKYAGIEPIES"
     gene            1140..>1501
                     /locus_tag="PLW80_07640"
     CDS             1140..>1501
                     /locus_tag="PLW80_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPB66417.1"
                     /translation="MVHFADETRITVSSGNGGNGCVAFRREAHVPLGGPAGGDGGRGG
                     DVVFEVRRNLRTLVYIRYAKHYRAQAGQDGMGRNRHGKDGEDVVIPVPPGTVIKDSGT
                     GETLMDFGTSGEGRWVFLR"
BASE COUNT          277 a          457 c          520 g          247 t
ORIGIN      
        1 gtacgccgtt ccgaggcgcc ggttcttggc cgggctcggc catgatcatt gtacgcgtcg
       61 gccgataccg gatggcaacg tccctcggcc tcggcatacg aaacataaga ggttgacagg
      121 atgtacgcaa tcgtagaagt gcaaggcaag cagtaccgcg ccgagaaggg cgccgttctc
      181 cgcgtggacc gcatggacgg cgaggccgga accccggtcg ccatcgattc tgttctgctc
      241 gtgtccggtg atacgatcaa gatcggcgct ccctacgttc agggcgccca ggtgaaggcg
      301 acgatcaagg cccaggagaa gggcgacaag gtcatcgtgt tcaagtacaa gcccaagaag
      361 gactaccgcc ggacccaggg ccataggcag cgctacacgc tccttacggt cgaggatatc
      421 gttggggcct gataggctta tcgttaggct atcgttctgg ccggacgggg cgctcaaggg
      481 cgcccgcgcc gacggacacg ccggcgaccg ccgggcgggc gccaacctgg cttgcgccgc
      541 agtcaccgtc ctcctgagga cggcgtacga gacggccgcc ggctacgaag gcgttacggt
      601 caggggcata gccccggcgc cgggtagcct gagcttcgag attgggcggt acccgcccgc
      661 gatggccgag aggctgcgcg gcgtcggtga cttcctgacc gtgggattgt ccagcgtgga
      721 tcgcgagtat ccgggcttga tagattttct attagagaga tgacggaggt tctatatgcc
      781 acataagggt aacggccgcg attcgaatcc gcagcatctg ggcgtcaagg cgtacggcaa
      841 cgagctcgtg cgcgcaggct cgatcatcgt gcgccagcgc ggcaccaaga tccatcccgg
      901 cgagaacgtc ggcaagggca aggacgacac gctgttcgcc aaggccgacg gccgggttgt
      961 attctgcgag caccgcggcc gcaagtacgc cggcatcgag ccgatagagt cctaaaggcc
     1021 tcgccgcggc gacgcggcca gcatcgagcg cgactttcgg atagtcgcgc ctcacgccgt
     1081 cgcgtccggc catcggccga gcggcggctt ttctatctcc ggaagggacg ggtagcgcga
     1141 tggtacattt cgccgacgag acgaggatca ccgtatcttc cggcaacggg ggcaacggtt
     1201 gcgtggcgtt caggcgcgag gcccacgtgc cgctcggagg cccggctggt ggcgacggtg
     1261 gccgcggcgg cgatgtcgtc ttcgaggtgc gacgcaacct gaggacgctc gtgtatatcc
     1321 gctatgccaa gcattaccgt gcccaggcgg gtcaggacgg catgggtcgg aatcgccatg
     1381 gcaaggacgg agaggacgtc gtcataccgg tacccccggg cacggtcatc aaggactccg
     1441 ggaccggcga aaccctcatg gacttcggca cttccggcga gggacggtgg gtcttcctgc
     1501 g
//
LOCUS       DAOOLN010000565         1501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_110308_length_1501_cov_3.242047, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000565 DAOOLN010000000
VERSION     DAOOLN010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110308_length_1501_cov_3.242047"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1501)
                     /locus_tag="PLW80_07645"
     CDS             complement(<1..>1501)
                     /locus_tag="PLW80_07645"
                     /inference="COORDINATES: protein motif:HMM:NF021610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HPB66418.1"
                     /translation="LELIEDLSGTRKSGLTFAVETPLEQWQMTLNKRAGIEKISRILA
                     EAEKRGYRVAKFYFMVGLPLPDAGVSEVEAIVSFIGELLAVAPTIKLNLTVATFVPKP
                     HTPFQWSPQLGYVEAASKIYAVKDAFKSNARVKVTYHTPFLSWLEGIVSRGDERVGEL
                     LIDAYRRGARFDAWDDLCDKDTWKAALEAAGESATRHMSGFDEAAELPWSDVSLRVSQ
                     KFLLDELKRSRDSSLTAACADACSDRCGSCDSSLSVGGDDEILERIAALSKAADLGSL
                     DAPVAAGRPLATRRVPAQGDRYRLFFRYAKEGRAAFFAHHELQTMLSSAIDRAGAPIA
                     YSEGFNPMPRLEISEPMSLGFESSDEYGSVILTGRLPVDPSDVASAANRLLHGDLRIL
                     EAYTLLCQDGKKFPSLSSVHWGSRYSVDLLSTGLDPRLLASAVRKRIQSDTRLADATV
                     GVDGDVVTLLLPFTGKRELGLPALFEDAYGFSIREARISVRRSAQYAKSA"
BASE COUNT          288 a          449 c          496 g          268 t
ORIGIN      
        1 gccgacttgg cgtactgggc agaacgacgg accgatatcc tggcttcgcg gatcgagaat
       61 ccgtacgcat cctcgaagag cgccggcagg cctaactcgc gcttgcccgt gaatggcagc
      121 aagagcgtca cgacatcgcc atcgacgccg acggtggcat cggcaagcct cgtatcggac
      181 tgtatacgtt tccgtactgc tgatgcgagt agcctcggat ccaagccggt actcaggaga
      241 tcgacgctgt agcgcgaacc ccagtggacg gaagacaggg atgggaactt cttgccatcc
      301 tggcataaca gcgtataggc ctcgagtatc ctgaggtctc catgcaggag gcggttggcg
      361 gcagacgcta cgtcggaggg atcgaccggg agccggccgg tcaatatgac ggagccgtac
      421 tcgtcgctcg actcgaaacc gagggacatg ggctcggaga tctcgaggcg aggcatcggg
      481 ttgaagccct cggagtacgc tatcggagca ccggcgcggt cgatggcgga gctgagcatg
      541 gtttggagtt catggtgcgc gaagaaggcc gccctgccct ccttcgcata ccggaagaac
      601 aaccgatacc ggtctccctg agccgggacg cgacgagtag ccagcggccg gccggcggct
      661 acgggcgcgt cgagcgatcc gaggtccgcg gccttggata aggccgcgat ccgctcaagt
      721 atttcgtcgt caccgcccac gctcagtgag ctatcgcagg agccgcagcg gtcagagcag
      781 gcgtcggcgc aagccgcggt cagcgacgag tccctggagc gtttcagctc atcgaggagg
      841 aacttctggg atacccgcag cgatacgtcc gaccacggaa gctcggcggc ttcgtcgaag
      901 ccggacatat ggcgcgtggc cgactctccg gcggcctcga gcgccgcctt ccaggtatcc
      961 ttatcgcaga ggtcgtccca ggcatcgaaa cgtgcgccac ggcgataggc gtcgatgagg
     1021 agttctccga cacgctcgtc tccccgggag acgataccct caagccagga aaggaacggc
     1081 gtgtggtagg tgaccttgac gcgggcgttg gacttgaacg catccttgac ggcatagatc
     1141 tttgacgcgg cctcgacgta gccgagctgt gggctccatt ggaagggcgt atgcggtttt
     1201 ggcacgaacg tcgcgacggt caggttgagc ttgatcgtag gggcgacggc tagcaattcg
     1261 ccgatgaacg agacgatggc ctctacctcg gacacgcccg cgtccggtag cggcagcccc
     1321 accatgaaat agaatttggc tactcggtag ccgcgtttct ccgcctcggc aagtatacgg
     1381 ctgatcttct cgatgccggc gcgcttgttc aacgtcatct gccattgctc gagcggcgtc
     1441 tcgaccgcga aggtcaggcc gctcttgcgg gtaccggaca ggtcctcgat gagttcgagc
     1501 g
//
LOCUS       DAOOLN010000566         1495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_111026_length_1495_cov_2.716667, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000566 DAOOLN010000000
VERSION     DAOOLN010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111026_length_1495_cov_2.716667"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..>1495
                     /locus_tag="PLW80_07650"
     CDS             55..>1495
                     /locus_tag="PLW80_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66419.1"
                     /translation="MAERSAWEAKAGELLLSGERAFGGASERLESSIAEARAEFERDA
                     AERGLASSARASAWADVYVQSGQAAMGAKSGAEYWLSSLGAVGLLVGSAELTAWLDGA
                     EAGASSSRLASIAKARECIERYDEFMARCSEARGRLIDDFGLAIGSGAGSLVDVLAEG
                     ADSLSFCLDEYQVELLRAEAVEGYWRSRVSIAEAVSAYAEDLSSGRATAGDTAAGLAA
                     ARSAYDAAASAYDQAKSDLSEAGSRIGEASEGIGFAFDALQESGLRLERAASEYSSLM
                     VELASGGNGEWLRSELLTRYRDLVESAGLSADTSAASEAELLAAYYERAVECGQFKAI
                     EAAGRAMRSAVEGVGEEPSLRTLANAAAAVVEPSSLYDWSSEGVGSGELFAAGYGLST
                     ADPAYASLLALHDHTASALTGASSDDRRAELIASSLELAARLARRADSLAESALEARL
                     DAVALLGAVDSAAWYGSRTGETRASSTCCL"
BASE COUNT          252 a          451 c          546 g          246 t
ORIGIN      
        1 gctacggcgt tcgcggacgg tggctcggca tggtccaagg cgtacaatcg tctcatggcg
       61 gaacggtcgg catgggaagc taaggccggc gagctactcc tgagcggcga acgggcgttt
      121 ggcggcgcta gcgagcggct cgagtcgtcc atcgccgagg cgagggccga gttcgagcga
      181 gacgccgcgg aacgggggct ggcaagctcc gcgagggcca gcgcctgggc cgacgtgtac
      241 gtccagagcg ggcaggcggc catgggcgcc aagtctggcg cagaatactg gctatcgtcg
      301 ctcggcgccg ttgggctact ggtcggctcg gctgagctga cggcttggct agacggcgcc
      361 gaggctgggg catcgtcgtc taggctggcc tcgatagcga aggcgcgcga atgcatcgaa
      421 cgttatgacg agttcatggc cagatgctcc gaggctcgag gtcggctgat cgacgacttc
      481 gggctggcca taggctcggg agccggctcc ttggtcgacg tgctagccga aggggcagac
      541 agtctgtcgt tctgcctgga cgagtaccaa gtggagttgc tcagggccga ggccgtcgaa
      601 ggatactggc gctcgagggt gtcaatagcg gaagccgtga gcgcctacgc ggaggatctg
      661 agctcgggca gggctaccgc cggggatacg gccgcaggac tggcggcggc gcggtcagcc
      721 tacgacgcgg cggcctcggc ctatgaccag gccaagtcgg atttatccga agcgggtagc
      781 cgtatcggcg aagcctccga gggcatcggc ttcgccttcg acgcgcttca agaatccggg
      841 ctgaggctcg aacgtgcggc gtccgagtac tcatcgctga tggtcgaatt ggcctccggc
      901 gggaacggcg agtggctgag atccgagctg ttgacccgat accgcgacct cgtcgaatcg
      961 gctggcttat cagccgacac gtcggccgcc agcgaagcgg agctgctcgc ggcgtactac
     1021 gagagggccg tggaatgcgg tcagttcaaa gcgatcgagg ccgcgggaag ggccatgcgt
     1081 tcagctgtcg aaggcgtcgg cgaagagccc agcctacgta cgttggcgaa cgccgccgcg
     1141 gccgtagtcg aaccgtcttc cctatacgat tggagctccg agggcgtcgg ttcgggggag
     1201 ctcttcgccg ctggctacgg gctgtcgacc gcggatccag cctacgcctc gctcctggcc
     1261 ctgcacgacc atactgcgtc ggcgctcaca ggcgcgtcta gcgacgacag aagagccgag
     1321 ctgatcgcct caagcctaga actcgccgct cgcctggcca gacgagccga tagcctggcc
     1381 gagtcggcgc tcgaggctcg ccttgacgca gtagccttgc ttggcgccgt cgactccgcg
     1441 gcctggtacg gctcgaggac cggagagaca agggcgagct cgacctgttg tctaa
//
LOCUS       DAOOLN010000567         1493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_111294_length_1493_cov_1.678025, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000567 DAOOLN010000000
VERSION     DAOOLN010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111294_length_1493_cov_1.678025"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PLW80_07655"
     CDS             <1..218
                     /locus_tag="PLW80_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66420.1"
                     /translation="GRVFGVLGMISSVMFPLGMLLFGPMADVVPIEWLLVGTGAAIFA
                     MGFSLLMSGALIAAGEGRGGRSGAAAE"
     gene            615..1121
                     /locus_tag="PLW80_07660"
     CDS             615..1121
                     /locus_tag="PLW80_07660"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPB66421.1"
                     /translation="MMLALERLARYNAVANQAMMDAMSLSGPGIFDEPVKGYYHSVHE
                     ILAHLYMADQLVLSELAASIGKERCGDEAAVPNDRPIVDLLQFRAARSELDACFLKLV
                     SILSENDLRAQVRKTVRSGETIERPLWVVLMHCFNHQTHHRGQVAQILDAFGVANDYS
                     GMLRLELP"
BASE COUNT          273 a          455 c          447 g          318 t
ORIGIN      
        1 tgggccgcgt cttcggcgtg ctcggcatga tatcgagcgt gatgttccct ttaggaatgc
       61 tactcttcgg ccctatggcc gacgtcgtgc ctattgaatg gcttctcgtc ggcacgggcg
      121 ccgccatctt cgcgatggga ttctccctcc tcatgagcgg ggccctgatc gccgcgggcg
      181 aaggacgagg cgggcggtcc ggcgcggccg ccgagtaaaa aaaaacgggg acggctcccg
      241 ccggtccccg cgtcgtctgt acataaaccc gaaccatccg gccagggctt actcgctgag
      301 cgcgaatcct ctataggtcg gaatcggcgc cttcggtccc gcctatgtct ccgcgttctc
      361 gctatcctgg acgcgctggg ccgatatgtc gtgttggtga cggcccgagc cgtagacgcc
      421 gataacggac ggagccgagc tgatcggatc tatcatcgcg ttaccctccc gtacctataa
      481 gcttatccat ccgccagacg aaatcaaggg cacggggccg attatcttca tcgtgcgtca
      541 gctgttttat tgatatacta tcacatctca aatcggacgg ccgatcaccg cagtcggccc
      601 gcaaggagtc tcgtatgatg ctcgccctcg agcgcttggc ccgctataac gccgtcgcca
      661 accaggctat gatggacgcg atgagcctat ccggccccgg gatcttcgac gagcccgtca
      721 aagggtacta ccatagtgtg cacgagatac tcgcccatct ctatatggcc gaccagctcg
      781 tcctatcgga gctagcagcg tcgataggca aggaacgatg cggagacgag gccgccgtcc
      841 cgaacgacag gcctatcgtc gacctgcttc agttccgcgc ggcccgctcg gagctggacg
      901 cctgcttcct gaagctcgtt tctatattga gcgaaaacga ccttcgcgcc caggttcgca
      961 agaccgtgcg ctcgggcgag accatcgaga ggcctttgtg ggtcgtgctg atgcattgct
     1021 tcaaccacca gacgcaccat cgcgggcagg tagcccagat cctcgatgcc ttcggagtcg
     1081 ccaacgacta ctccgggatg ttgcgcctgg agctaccata acgcattcat agcaccgttc
     1141 ggtttcagta gtaggccctc cggtgttggg agctgcgttc tcgctgggct ccgcaacgcg
     1201 ctatcgatgc cgagatcgct acgcgctatg accgggaggg cgacggccgc ttcgatcggt
     1261 gagaatccga gaggatacgt cggacgcgtt cgcaagaatc gaaggctatc actgctttat
     1321 ccagatcgat atcgatggcg tcgtctaatc ctatggtggg ggtttcggta atcccgactg
     1381 cgctattagc ttatgggtcg ccgaaccagc gccgggagag gcctcttggt cgagccatgt
     1441 gtcggtctcc gggttcgagg attatgggga gcggccttgg catatgtcgg ctt
//
LOCUS       DAOOLN010000568         1492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_111336_length_1492_cov_3.431454, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000568 DAOOLN010000000
VERSION     DAOOLN010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111336_length_1492_cov_3.431454"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /gene="thyX"
                     /locus_tag="PLW80_07665"
     CDS             <1..691
                     /gene="thyX"
                     /locus_tag="PLW80_07665"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HPB66422.1"
                     /translation="GTKTVREDAGLIDYLLRNEHTSPFEQVVLTFHIKMPIFVARQWI
                     RHRTARVNEISGRYSVMKDEFWIPAPEAVARQSADNKQGREVDPLSPDEAARVIGLLE
                     EGQRSAYASYKAMVDSGLARELARVNLPLSLYTEWYWQIDLHNLFRFLALRLDAHAQR
                     EIRDYAAVILDMTRKVCPLACASFERHLLAGVRLSAEEASAFRRLLDGGEAGLGGREL
                     ERLEEKLAKVR"
     gene            765..>1492
                     /locus_tag="PLW80_07670"
     CDS             765..>1492
                     /locus_tag="PLW80_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB66423.1"
                     /translation="MPENVIEARGLTRTYRKGPRALAGVDLDVPEACRFALLGANGAG
                     KSTLVRILCTLSRQDSGRVAIAGRPISGGSQAIRALIGVALQDTQLDPAESARRQLAF
                     QGRLYGFDAARAAGRAEELLARFGLEPVADRKAAELSGGNKRRLHVALALVHRPRVLF
                     LDEPTVGMDPESRAAFWAEMRRLNAEEGACVFFTTQYLEEAEKHGDELAVLDSGLVAY
                     RGTVDGFAAAHAGPGGDLEAGYLN"
BASE COUNT          250 a          486 c          521 g          235 t
ORIGIN      
        1 gggcacgaag accgtgcgcg aggacgccgg cctgatcgac tacctgttgc gtaatgagca
       61 tacctcgccg ttcgagcagg tcgtgctgac cttccatatc aagatgccga tcttcgtcgc
      121 gcgccagtgg atacgccacc gtaccgcgcg cgtgaacgag atatccggcc gctactcggt
      181 gatgaaggat gaattctgga ttccggctcc cgaggcggta gccaggcaga gcgccgataa
      241 taagcagggc cgcgaggtcg atcctctgtc cccggacgag gccgctcgcg tcatcggcct
      301 gctcgaggag ggacagaggt cggcctacgc ctcatacaaa gcgatggtcg actcgggcct
      361 ggcccgagaa ctggcccgcg tcaacctgcc cctgtcgctg tacaccgagt ggtactggca
      421 gatagacctg cacaacctgt tccgcttcct ggcgctgagg cttgacgccc acgcgcaacg
      481 ggagatccgc gactatgcgg cggtgatcct cgatatgacg cggaaggtct gtcccctcgc
      541 ctgcgccagc ttcgaacggc acctgctcgc tggagtcagg ctgtcggccg aggaggcgtc
      601 ggccttcagg cggctgctcg acggcggcga ggcggggctc ggaggccgcg agctcgagcg
      661 cctggaagag aagctggcca aagttcgcta aaaggaaacc ctccagtagt gcaccgaccc
      721 gctgccagcg ccgtatcctc ctctatcata gaggaggata cgaaatgcca gagaacgtca
      781 tcgaggcccg cgggctgacg cggacctatc gcaaggggcc tcgggccctc gccggggtcg
      841 acctcgacgt ccccgaggcc tgccgcttcg cgcttctggg tgccaatggc gccggcaagt
      901 cgacgctcgt gcgcatcctg tgcacgctgt cgcgccaaga ctcgggacgc gtcgcgatcg
      961 cggggcggcc gatctcgggc ggctcgcagg ctatccgcgc cctgataggc gtggcgctcc
     1021 aggataccca gctagaccca gccgagagcg cgcgccggca gctagcgttt cagggccgac
     1081 tgtacggctt cgacgcggcc cgggccgccg ggcgggcaga agaactgcta gcgcggttcg
     1141 ggctggagcc tgtcgccgat cgaaaggccg ccgagctgtc cggcggcaac aagcggcgcc
     1201 tacacgtggc gttggccctc gtccaccggc cccgcgtcct ctttttggac gagccgacgg
     1261 tcgggatgga tcccgagagc cgcgcggcgt tctgggcgga gatgcgtcgg ctgaacgccg
     1321 aggagggcgc gtgcgtcttc ttcacgacgc agtatctaga agaggcggag aagcacggcg
     1381 acgagctggc ggtactggac tcaggcctgg tggcgtaccg gggcacggtg gacggcttcg
     1441 cggcggcgca cgccggcccc ggcggggacc tcgaggcggg ctacctgaat tt
//
LOCUS       DAOOLN010000569         1491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_111446_length_1491_cov_3.700557, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000569 DAOOLN010000000
VERSION     DAOOLN010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111446_length_1491_cov_3.700557"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..409
                     /gene="infA"
                     /locus_tag="PLW80_07675"
     CDS             188..409
                     /gene="infA"
                     /locus_tag="PLW80_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPB66424.1"
                     /translation="MAKEEAIEVEGIVREALPNTMFRVELKNGGHLILAHLSGKMRKH
                     YIRIVPGDTVRVALSPYDLSRGRIIYRER"
     gene            complement(406..810)
                     /locus_tag="PLW80_07680"
     CDS             complement(406..810)
                     /locus_tag="PLW80_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPB66425.1"
                     /translation="MDFGEILKLWDAKTGAAALAEAGRGDGKDGPKERRLTKKELDAL
                     PVDARLDLHGLTAAEAEDALASFFAAAERGGCRKLMIVHGKGLHSASEPVLAGVVRRW
                     LERRPSAGKSGHPDSANGGTGATWVLLKERED"
     gene            complement(832..>1491)
                     /locus_tag="PLW80_07685"
     CDS             complement(832..>1491)
                     /locus_tag="PLW80_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66426.1"
                     /translation="GVAAAVSAAGSPDWGAALDEATRAAASDGGTPRERAALAALSLL
                     ADTGSERDRAAAYAELKRLGRAACILPGLPAALAALDESFGNLDDGANVLPPFGYPAA
                     GAVVLSAAAIAAILRRRASDRRASGHGSRRRLSLPLGLAVAGAALAAFAALAAAEEAR
                     PRFVCLGGVARTVPSAEAGGSAIAAGRGGRVLESAGGWLFVEPAGGSAGWLAADDAVV
                     Y"
BASE COUNT          252 a          549 c          474 g          216 t
ORIGIN      
        1 aagcgtcagc gtgggcgccg tcggcgccgg atcgtcatag aaacgagcga gttactattc
       61 atggattgcg tgtctctgtc aaccatccgg acgggcaggt tgacacgcca acgcgcggcc
      121 cgtactatta tagcgcttcg gacgccgcgc tcaagcgaga gcgtcccatt tctccgtagg
      181 aggcatcgtg gccaaggaag aagctatcga ggtagagggt atcgtcaggg aagccctgcc
      241 gaacaccatg ttcagggtcg aattaaagaa tggcggacac ctcattctag cccacctttc
      301 cggcaagatg cgcaagcact acatccgtat cgtccccgga gacacggtcc gggtagcctt
      361 gtcgccgtac gacctatccc gagggcgcat catctatcgc gagcgctaat cctcccgttc
      421 cttcaataga acccaggtag ctccagtccc tccattggcc gagtccgggt ggccggattt
      481 gccggccgag ggcctgcgtt ccagccaacg gcgtacgacg cccgccaaca cgggctccga
      541 cgccgaatgc aggcccttgc cgtggactat catcagcttg cgacagcctc cgcgctccgc
      601 cgccgcgaag aaggacgcca acgcgtcctc cgcctccgcg gcggtcagtc cgtggaggtc
      661 aagcctcgcg tccacgggca aggcgtctag ctccttctta gtcaggcggc gctccttcgg
      721 cccgtcctta ccgtcaccgc gtccggcttc ggctaacgcg gcggccccgg tcttcgcgtc
      781 ccacaacttg agtatctcgc cgaagtccac ctcgcgcccc ttcccgccgg cctaatatac
      841 cacggcgtcg tcggccgcca gccacccggc cgaaccgccg gccggctcga cgaacagcca
      901 gccgccggcc gactcgagca cgcgcccgcc gcggcccgct gctatagccg agccaccggc
      961 ctcggccgac ggcacggtcc tcgctacgcc gccgaggcag acgaagcggg gtcgagcctc
     1021 ttccgccgca gccagggcgg cgaaggcagc cagcgcggct cccgcgacgg ccaggccgag
     1081 cggcaatgag agccgacgac gcgagccgtg accagaggcg cggcggtccg aggcgcgacg
     1141 ccggagtatc gcggcgatgg cggccgccga caggacgaca gcgcccgccg ccggataccc
     1201 gaacggcggt aaaacattcg cgccgtcgtc gaggttaccg aacgactcgt cgagcgccgc
     1261 cagggccgcg ggaagccccg gtaggataca ggccgctctc cccaaccgtt tcagctcggc
     1321 gtaggcggcg gcgcgatcgc gctcggagcc cgtatcggcc agcaggctta gcgcggctag
     1381 cgccgcgcgc tcgcgcggcg ttccgccgtc ggacgccgcg gcgcgagttg cctcatcgag
     1441 ggccgcgccc cagtcgggac ttcctgccgc cgagacggcg gccgcaaccc c
//
LOCUS       DAOOLN010000570         1490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_111613_length_1490_cov_2.726132, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000570 DAOOLN010000000
VERSION     DAOOLN010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111613_length_1490_cov_2.726132"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLW80_07690"
     CDS             complement(<1..211)
                     /locus_tag="PLW80_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66427.1"
                     /translation="MDVNTLLVTIALSFLPISELRGAIPFAVARGAPLLEAAAIAVAC
                     NALVGPVALDRALQLALDLAPHPAGL"
     gene            338..>1490
                     /locus_tag="PLW80_07695"
     CDS             338..>1490
                     /locus_tag="PLW80_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPB66428.1"
                     /translation="MPEPTPPSLPDSFHPLVRSWFAGRYGEPTPVQAAAWTVIASGGH
                     VLASAPTGSGKTLTAFLGALSRFASGEYPPDGLSVLYVSPLKALNEDVRLNLLEPARE
                     LAVWFRDRGQAFPRLRIASRSGDTSQSERRGMIARPPAILCVTPESLAIMIASRSGLA
                     MLSSVRLLILDELHAVLGAKRGSALACSVGRLALLAGEFQRVALSATVEPFDEAARFA
                     AGSRLVRGPDGLARYEPRTMAVVAPPASKRYELSVAWPAAPLRVRPPSAGPDERETDD
                     RMIDEPAGRYDAIADELAGRLERERSTIVFADSRRRAERLAAMLNERSGDGTAWAHHG
                     SLSKDVRKAVEARLKDGRLRCVVATSTLELGIDVGAIDLVALAGSPQHAD"
BASE COUNT          206 a          561 c          507 g          216 t
ORIGIN      
        1 tcaggcctgc tgggtgcggg gcgaggtcaa gcgcgagctg gagcgcgcga tcgagcgcta
       61 ccgggccgac gagggcgttg caggcgaccg ctatcgcggc cgcctcgagt agaggcgccc
      121 cgcgggcgac ggcgaatggt atggcccctc gtagttccga tatcggcagg aaggacaggg
      181 ctatggtaac gagcagcgtg tttacgtcca ttggccggag tatagccgga agccgcgacg
      241 ccggacaagc gccgtcgcca gcgacgctgg taccccagcg acgcttgtac gccaagcgac
      301 gcttgagcgt acgcgggcga tcgcgtacta tcgtcccatg cccgaaccga cgccgccatc
      361 cctacccgac tcgttccacc cgctcgttcg ctcctggttc gccgggcgct acggcgagcc
      421 gacgccggtg caggccgcgg cctggaccgt catcgcctcg ggcggacacg tcctcgcctc
      481 cgcgccgacc ggctcgggca agaccctgac cgccttcctc ggcgccctgt cgcgcttcgc
      541 ctcgggcgag tacccgccgg acggcctgtc ggtgctctac gtgtcgccgc tcaaggcgct
      601 caacgaggac gtcaggctca acctgctcga gccggccagg gagctggccg tctggttccg
      661 cgaccgggga caggccttcc cgcggctcag gatagcctcg cgctcgggcg acacgagcca
      721 gtccgagcgc cgcggcatga tagccaggcc gcccgcgata ctctgcgtga ccccggagag
      781 cctggccatc atgatcgcct cgcgttccgg gctggccatg ctaagctcgg tcaggctcct
      841 gatactcgac gagctccacg cggtcctcgg cgccaagcgc ggctcggcgc tcgcctgctc
      901 cgtcggcagg ctcgccctgc tcgcgggcga gttccagcgc gtcgccctgt cggctacggt
      961 cgagccgttc gacgaggcgg cccgcttcgc cgcgggctcg cgcctggtac gcggcccgga
     1021 cggcctagcc cgctacgagc ccaggactat ggccgtcgtc gctccgcctg catccaagcg
     1081 ctacgagctg tcggtcgcct ggcccgcggc gcctctgcgc gtacgccccc cgtcggccgg
     1141 ccccgatgag cgcgagaccg acgatcgcat gatcgacgag ccggccggcc gctacgacgc
     1201 gatagccgac gaactggccg ggcgcctgga gcgcgagcgc tcgaccatcg tcttcgccga
     1261 ctcgcgccgg cgggccgagc ggctcgcggc catgctcaac gagcgctccg gcgacggcac
     1321 ggcctgggcc caccacggtt ccctgtccaa ggacgtacgc aaggccgtcg aggccagact
     1381 caaggacggc cgcctgcgct gtgtcgtcgc gacgagcacg cttgagctag gcatcgacgt
     1441 cggcgctatc gacttggtcg cccttgccgg ctcgccccag cacgccgacc
//
LOCUS       DAOOLN010000571         1489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_111701_length_1489_cov_3.423291, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000571 DAOOLN010000000
VERSION     DAOOLN010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111701_length_1489_cov_3.423291"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1390
                     /locus_tag="PLW80_07700"
     CDS             <1..1390
                     /locus_tag="PLW80_07700"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HPB66429.1"
                     /translation="SGLGRPLRNTLSVVLDGEVVFEQAKTLLPTYDVFDEARYFEPAS
                     ERRLWRHRGVAVGFAICEDLWRETAPVPGMRYPVDPPRELLDAGADLIVVPSASPFQL
                     DKSALRVGLCSDLARTGSVPVAYCNMAGADDSLVFDGRSFLVDRTGRLVALGVFGEGI
                     LAVDTDECADEATIAAFPDRWEEAERVLVEGIRGYMRKCGFKKAHLGLSGGIDSAIVA
                     VLAAKAIGPENLTCIAMPSRFSSAGSIGDSVELAANLGCRLETIPIEGPFSSFLATME
                     PHFAGLPFGLTEENLQARVRGDILMAWSNKFDSMLLTTGNKSELAVGYCTLYGDMCGA
                     LAPIGDLLKTEVFELCESVYAREGLLPRAIIDKPPSAELRPNQLDQDSLPPYELLDAV
                     LRLYIEDDLAVDAIVALGFDEELVRRVVSMTARAEYKRRQAAPVVKVSPRAFGMGRRM
                     PIARVVSEARPR"
BASE COUNT          211 a          513 c          502 g          263 t
ORIGIN      
        1 ctcaggcctc ggcaggccgc tacgcaacac gctgtcggtc gtcctcgacg gcgaggtggt
       61 cttcgagcag gccaagaccc tgctgcccac ctatgacgta ttcgacgagg cgcgctattt
      121 cgagccggcc tccgagcgtc ggctctggcg ccatcgcggc gtcgcggtcg gcttcgccat
      181 ctgcgaggac ctatggcggg agaccgcccc ggtccccggt atgcgctacc ctgtggatcc
      241 gccgcgagag ctgctcgacg ccggcgccga cctgatcgtc gtgccgtccg cctctccgtt
      301 ccagctggat aagtcggccc tgcgcgtcgg actctgctcc gacctggcca ggacaggctc
      361 ggttccggtc gcctactgca acatggccgg tgccgacgac tcgctcgtct tcgacggccg
      421 gtccttcctc gtcgaccgca caggccgcct cgtcgcgctc ggggtcttcg gtgagggcat
      481 actcgccgtc gataccgacg aatgtgccga cgaggccacg atcgccgcct tcccggaccg
      541 gtgggaggag gccgagcgcg tcctcgtcga gggtatccgc ggctacatgc gcaagtgcgg
      601 cttcaagaag gcccacctcg gcctgtccgg cggcattgac tcggccattg tcgcggtgct
      661 ggccgccaag gctatcgggc cggagaatct gacctgcatc gccatgccgt cgcgcttctc
      721 gtcggccggc tccataggcg acagcgtcga gctcgcggcc aacctgggct gccgcctgga
      781 gacgatacct atagaggggc cgttctcgag tttcctggcg acgatggagc cgcacttcgc
      841 gggcctgccg ttcggcctga ccgaggagaa cctgcaggcg cgcgtccggg gcgacatcct
      901 gatggcttgg tccaacaaat tcgattcgat gctcctgacg accggtaaca agagcgagct
      961 ggccgtcggc tattgcacgc tgtacggcga tatgtgcggc gctctggctc cgatagggga
     1021 tctgctcaag accgaggttt tcgagctgtg cgagagcgtc tatgcgcgcg aggggctcct
     1081 gccccgcgcc atcatcgaca agcccccgtc cgccgagctc cggcccaacc agctcgacca
     1141 ggactcgcta ccgccttacg agctgctcga tgcggtgctc aggctctata tcgaggatga
     1201 cctggccgtg gacgccatcg tcgcgctcgg cttcgacgaa gagctcgtcc gaagggtcgt
     1261 atcgatgacg gcgcgcgccg agtataagcg cagacaggcg gccccggtcg tcaaggtgtc
     1321 tccgcgggcc ttcggtatgg ggcggcgtat gcccatcgcg cgcgtcgtat ccgaggcaag
     1381 gcctcggtga taggcccggc ggtcctggcc gcggcgttgg ccggagcgtt gctcgcctcg
     1441 tgcggccgag cgcccgaggc ggaggccggc ccgggtcccg ccgtatcgg
//
LOCUS       DAOOLN010000572         1489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_111721_length_1489_cov_2.899582, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000572 DAOOLN010000000
VERSION     DAOOLN010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111721_length_1489_cov_2.899582"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PLW80_07705"
     CDS             complement(<1..652)
                     /locus_tag="PLW80_07705"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPB66430.1"
                     /translation="MKLYRIYNVYCPLDHDERYLRERAAKELGVRPGHLAAFGIERRS
                     IDARDKRAVRFVYTLTAGLESPSRSLGAKAAELAPAEPYRFPAPSVEPSGKEGHRPII
                     VGAGPAGLFCALMLAEAGYRPIVIERGDAVDKRGLAVSSFWNGGELDPESNMQFGEGG
                     AGTYSDGKLTTSSGDGSGRAAQVLDELVAAGAPESIRFDGKPHIGTDTLVEVVKGLR"
     gene            736..>1489
                     /locus_tag="PLW80_07710"
     CDS             736..>1489
                     /locus_tag="PLW80_07710"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPB66431.1"
                     /translation="MKKLRIGELSGLVGANASALRFWEERGITEPEKDSDNSYRLYSA
                     EDSCRFLRARRYRSFGFSVDDAKAMIGAAVPEATDGLLARRLAELDEEARRAAALRDE
                     LARYGRACSRARLEVGRFEPDVLAAASYIFTIERGSFVGDSARLELSKRWTERLPFAT
                     FALYVPGEAFSGVGEYHVSWGFGLGDDQPGSRAIVGSARAAGMDILTLEDRRCVRTAF
                     YRDDPRDMRPGELSPIVDAFRAAGYEATGPAIG"
BASE COUNT          218 a          500 c          527 g          244 t
ORIGIN      
        1 ggcgcaggcc cttgaccacc tcgaccagcg tgtcggtgcc gatatggggc ttgccgtcaa
       61 accgtatcga ctcgggcgcg cccgcggcca cgagctcatc gaggacctgg gcggcgcggc
      121 cggacccgtc gccgctcgag gtcgtcagtt tgccgtccga gtaggtgccg gcgccgccct
      181 cgccgaactg catgttcgac tccgggtcga gctcgccccc gttccagaac gacgatacgg
      241 ccaggccgcg tttatcgacc gcgtcgccgc gctcgatgac gatgggccga tagccggcct
      301 cggcgagcat gagcgcgcag aagaggccgg ccggacccgc tccgactatg atcggtcggt
      361 gcccttcttt tcccgacggc tcgacggacg gcgccgggaa gcggtacggc tcggccgggg
      421 ccagctctgc cgccttggcc ccgaggctgc gggacggcga ctcgagcccc gcggtgagcg
      481 tatagacgaa gcggacggcg cgcttgtcgc gcgcgtcgat cgagcggcgc tcgatcccga
      541 aggccgcgag gtggcccggg cggacgccga gctccttggc cgcgcgctcg cggaggtagc
      601 gctcgtcgtg gtcgagcggg cagtacacgt tatagatgcg atagagcttc atggctccag
      661 tatacacgaa cgcgattcgc gatgctcgct cggaagactt caaatccgag cacttttgtc
      721 gtagaatcgc gatcaatgaa gaagctgagg ataggcgagt tgtccggtct cgtcggcgcg
      781 aacgcgtcgg cgctgcgctt ctgggaggaa cggggcatca ccgagccgga gaaggactcg
      841 gacaatagct accgcctcta ttccgcggag gattcgtgcc gtttcctgag ggcccggcgc
      901 tatcgcagct tcggcttctc ggtggacgac gccaaggcga tgataggcgc cgccgtgccc
      961 gaggccaccg acgggctcct ggccaggcgg ctggccgagc tggacgagga ggccaggcgg
     1021 gcggcggccc tgcgcgacga actggcccgg tacggccgcg cctgttccag ggcccgcctg
     1081 gaggtcggac gcttcgagcc cgacgtcctg gccgcggcca gctatatctt tacgatagag
     1141 cgtggctcct tcgtcggcga ctccgccagg ctggagctat ccaagcgatg gaccgagcgg
     1201 ctaccgttcg ccaccttcgc gctgtacgtg cctggcgagg ccttctcggg agtgggcgag
     1261 tatcacgtct cctggggttt cggtctgggc gacgatcagc ccggctcccg ggccatcgtc
     1321 gggtcggccc gcgccgccgg catggatatc ctgaccctcg aggatcggcg ctgcgtccgt
     1381 accgccttct atcgcgacga tccgcgcgac atgaggcccg gcgagctgtc ccccatcgtg
     1441 gacgccttcc gggctgccgg gtatgaggcc acggggccgg ccatcggac
//
LOCUS       DAOOLN010000573         1489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_111746_length_1489_cov_2.276151, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000573 DAOOLN010000000
VERSION     DAOOLN010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111746_length_1489_cov_2.276151"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PLW80_07715"
     CDS             complement(<1..668)
                     /locus_tag="PLW80_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66432.1"
                     /translation="MLGSYPVRVLFCSSGVAGSGHVALGLSLGNALRRRARAGRAAEA
                     EYRLVCVDSPFAPLASRLGFDIVTVPPEGEAELGPGAYEGSALYRAIVDYAPDLLVVD
                     LFWFPLDSFIASLPCRKVLLIRQVDPRFFTLPLRDRELRLRPGDYDLVLRTEPCFETP
                     FPSEEIDPIIILDRGEIMGRDEALADLGLAPDERACLFAFNGAEGEGALAWKSFSYLE
                     DEGW"
     gene            complement(662..1423)
                     /locus_tag="PLW80_07720"
     CDS             complement(662..1423)
                     /locus_tag="PLW80_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66433.1"
                     /translation="MQFEFDVLDFALCSSTNRVYSVLYSVYSSNSFYFIRFVDLDDAE
                     PTAVDASSMDAYQIQQGLIAVDGLGYLYAVANEVATEREGLLRFSIDSTATATPIAFA
                     SFEELGLGYFTGLTNTIVQDMTIANGVLVVALSEIQPSYYATDLRSRGMIVGISPNSM
                     TNVWTAAAISESSPQYPIDPETQLYGPRRFLAVSPKRLYVLDAGFSWSGEAADPLKNE
                     MRVVAVNLETGAIAETGLNGQVDYFETDYSTYYMC"
BASE COUNT          310 a          446 c          459 g          274 t
ORIGIN      
        1 ttccagccct cgtcctccag gtaggagaag cttttccagg ccagggcgcc ctcgccctcc
       61 gcgccgttga acgcgaacag gcaggcccgc tcgtcaggcg ccaggccgag gtcggccaag
      121 gcctcgtcgc ggcccatgat ctcgccccgg tcgaggatga tgatagggtc gatctcctcg
      181 gacgggaacg gggtctcgaa gcacggctcg gtgcgcagga ccaggtcgta gtcgcccggc
      241 cgcaggcgca gctcgcggtc ccggagcggt aacgtgaaga agcgcggatc gacctgcctg
      301 atgagcagga ccttcctgca cggaaggctt gctataaacg agtcgagggg gaaccagaac
      361 aggtcgacga cgagcaggtc cggcgcatag tcgacgatgg cccggtagag ggccgagccc
      421 tcgtaggcgc cggggccgag ttcagcctcg ccctccggcg gcacggtgac gatatcgaag
      481 ccgagccttg aggccagcgg ggcgaacggc gagtcgacgc acacgagccg atactccgct
      541 tccgcggcgc gccccgcccg agcgcgccgc ctgagcgcgt tgcctagcga cagcccgagc
      601 gcgacatgac ccgaccccgc cacgccgcta gaacagaaga ggacgcgcac cgggtacgac
      661 cctagcacat gtagtacgtt gagtagtcgg tctcgaagta gtcgacttgg ccgtttaatc
      721 cggtttcggc gatagccccg gtctcgaggt tgacggcgac gactcgcatc tcgttcttca
      781 gcgggtccgc ggcctctccg gaccacgaga atcccgcgtc tagcacgtag agccgcttcg
      841 gggataccgc caggaagcgc cggggcccat atagttgagt ctcaggatcg ataggatatt
      901 gaggggacga ctcactaatg gccgcagcag tccaaacgtt cgtcatagag ttcgggctaa
      961 tgcctacgat cataccgcgg cttctaaggt ccgtcgcata atagctaggc tgtatctcgc
     1021 tcaacgctac gacgagtaca ccgttggcga tcgtcatgtc ctggactatc gtattggtta
     1081 gtcctgtaaa ataccctagg cctagctcct cgaacgatgc gaacgcgatc ggggtggccg
     1141 tcgctgtact gtctatggag aaacgcagta gtccctctcg ctcggtggcg acctcattgg
     1201 ccacggcgta caggtagccg aggccgtcta cggcgatgag gccctgttgg atctggtacg
     1261 cgtccataga agacgcgtcc actgctgtgg gttcggcgtc gtcgaggtcg acgaatcgta
     1321 taaaatagaa gctgttgcta ctatatacgc tgtacaatac gctgtacacg cggttcgttg
     1381 aggaacacaa cgcaaaatcc aagacatcga attcgaactg cacggtagaa taaggtgtac
     1441 ttgtcaacat cagatctgac acttagctaa aaatttaaag ctgttcact
//
LOCUS       DAOOLN010000574         1488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_111851_length_1488_cov_2.579902, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000574 DAOOLN010000000
VERSION     DAOOLN010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111851_length_1488_cov_2.579902"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="PLW80_07725"
     CDS             <1..129
                     /locus_tag="PLW80_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPB66434.1"
                     /translation="GVSRQTIISIEGGRYDPSLPLALKLARLLGQSIEDLFIDQEE"
     gene            131..541
                     /locus_tag="PLW80_07730"
     CDS             131..541
                     /locus_tag="PLW80_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66435.1"
                     /translation="MGFFGNLTMRRYAATNLILGAMFINLLLFGGRLEMGEAFTAGLA
                     TGLGVGWLAFGIGGWIALKRPGRTWDERTATINMKASAISFCVVMLVTALMSAALRSR
                     TLGMDWSAADLAGMLVNLGLASFGLSALVIRKRT"
     gene            609..1169
                     /locus_tag="PLW80_07735"
     CDS             609..1169
                     /locus_tag="PLW80_07735"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPB66436.1"
                     /translation="MDRCGLVDVATLGAGIIVDLRYATSDNFLGTRLYDGRSAWLLEP
                     AAAKLAVAAAHAATDGRSIVVLDAYRPLSAQALMWAARPEPGFVAPPERGSNHNRGAA
                     VDVTLADTDGRELPMPSAFDEFSERASHAYSGGPPDALANRDALRSYMERAGFVAYEA
                     EWWHYNDPESKSGPLLDVPFAELEGR"
     gene            complement(1243..>1488)
                     /locus_tag="PLW80_07740"
     CDS             complement(1243..>1488)
                     /locus_tag="PLW80_07740"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPB66437.1"
                     /translation="HLFADNGYAAGYYVYMWAEVLDADGFEAFEASGDVFNADLAAKF
                     KRFVLSAGNTLDPAEAYRAFRGRDPDVGALLRGRGLS"
BASE COUNT          243 a          502 c          494 g          249 t
ORIGIN      
        1 ggcgtttcaa ggcagaccat catctcgatc gagggcggcc gctacgaccc gtcgctcccg
       61 ctcgccttga aactcgccag actactcggg cagagcatcg aagacttgtt cattgaccag
      121 gaggaataag atgggatttt tcggaaacct gaccatgcgc cgctatgcgg ccaccaacct
      181 gatcctcggc gccatgttca tcaacctgtt gcttttcggc ggccggctcg agatgggcga
      241 ggccttcacc gccggcctgg ccaccggcct cggcgtcggc tggctggcct tcgggatagg
      301 cgggtggata gccttgaagc gccccggacg gacctgggac gagcgcacgg cgaccatcaa
      361 catgaaggcg tcggcgatat cgttctgcgt cgtcatgctc gtaacggcgc ttatgtccgc
      421 ggcgctgcgt tcccgcaccc tcggcatgga ctggagcgcg gccgacctgg cgggcatgct
      481 cgtcaatctc gggctggcga gcttcggcct ttcggctttg gtcatccgga aacgtacata
      541 gacccgaagc gggcctcggg tggcaccgga gctgtacacg gcggccgccc gagggtagca
      601 tcgtcgccat ggacagatgc ggattggtcg acgtagcgac gctgggagcc ggcatcatcg
      661 tcgacctgcg ctatgcgacg agcgataatt tcctggggac gcggctgtac gacggccgga
      721 gcgcctggct cctcgaaccg gcggcggcca agctggccgt cgccgcggcc catgccgcta
      781 ccgacgggcg atccatcgtc gtgctcgatg cctaccgtcc actatccgcc caggcgctca
      841 tgtgggccgc gcggccggaa cccggtttcg tagccccgcc ggagcgcggt tcgaaccaca
      901 accgcggcgc cgccgtcgat gtgacgctgg ccgacacgga cggccgagag ctacccatgc
      961 cgagcgcctt tgacgagttc tccgagcgcg cgtcgcacgc ctactcgggg ggccctccgg
     1021 atgccctggc caatcgggac gcgctacgct cgtacatgga gcgggccggc ttcgtcgcct
     1081 acgaggccga gtggtggcat tataacgatc cggagtcgaa gtccggcccc ctgctcgacg
     1141 taccgttcgc cgagctcgag ggacgatagg ccgacgcgga ctgcaactgg tcgtacagag
     1201 aacggggccc gcgaccgcct gagcgatcac gggccctgta tttcacgaca gccctcgtcc
     1261 ccgtagcagg gcgcccacgt cgggatcccg tcccctgaag gcgcggtacg cctccgcggg
     1321 gtcgagcgtg ttgccggccg acagcacgaa gcgcttgaac ttggcggcca ggtcggcgtt
     1381 gaacacgtcg ccggacgcct cgaaggcctc gaagccgtcc gcgtcgagca cctcggccca
     1441 catgtatacg taatagccgg ccgcatagcc gttgtcggcg aacaggtg
//
LOCUS       DAOOLN010000575         1488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_111873_length_1488_cov_2.272854, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000575 DAOOLN010000000
VERSION     DAOOLN010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111873_length_1488_cov_2.272854"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PLW80_07745"
     CDS             complement(<1..523)
                     /locus_tag="PLW80_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HPB66438.1"
                     /translation="MSDPEKNATIGSEAGGPGLDFIREAVAEDLRSGRFAYVRTRLPP
                     EPNGWMHIGHAKAFSIDYYVAKDFGGTCNLRFDDTNPAKEDMSYVEAIKRDTFWLGLD
                     CRDRVLFASDYYERMYELAVKLIKKGVAYVDDLSAEQMSEYRGTAVADKNNVTHTPPG
                     RDSPYRNRSVEENL"
     gene            complement(591..>1488)
                     /locus_tag="PLW80_07750"
     CDS             complement(591..>1488)
                     /locus_tag="PLW80_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HPB66439.1"
                     /translation="HLANVVDDHLMGITHVMRAQEWIPSAPLHMIMYEAFGWEPPRLC
                     HLPMVLGDDGHKLSKRHGATAVDEFRRRGYLEAALINYVAMLGCSYEDGRDLYSLEDL
                     ARLFSMDHINKAPAVFDYVKLEWFNGQYIRMLSDEELAARITPFMAEAGLVSAKPSAL
                     EVARLLAAAPLVKERLKYLSDAPEALRFLFAEPAVPPVEELLPKKMDAAGAARALGLA
                     MELLEANDPADHDACEAAFRSGGAEAGLKLGDLLMPLRVAVTGTKVSPPLFQSIAVLG
                     KATAIQRAGKALAALKAANERP"
BASE COUNT          229 a          477 c          511 g          271 t
ORIGIN      
        1 cgagattctc ctcgacggag cgattacggt acgggctgtc gcgaccgggt ggcgtgtggg
       61 tgacgttgtt cttgtccgcc acggcggtgc cgcggtactc gctcatctgc tccgcgctca
      121 ggtcgtcgac gtaggcgacg cctttcttga tcagcttgac ggccagctcg tacatccgct
      181 cgtagtaatc cgacgcgaac aggacgcggt cgcggcagtc gaggccgagc cagaaggtat
      241 cgcgcttgat cgcctcgaca taggacatgt cttctttagc aggattggtg tcgtcgaagc
      301 gcaggttgca ggtgccgccg aagtccttgg ccacgtagta gtcgatgctg aaggccttgg
      361 catggccgat gtgcatccag ccgttcggct cgggcggcag gcgcgtccgc acataggcga
      421 agcggccact cctcaggtcc tcggcgacgg cctcgcgtat gaagtcgagg ccgggaccgc
      481 cggcttccga ccctatcgtc gcgttcttct cgggatcgct catgggtagc tccttgtacc
      541 gcgcttcagg ggcgcggccg agtcagtatg ccccgcgctc ccgaaggcgg tcaaggacgc
      601 tcgttggcag ctttgagcgc ggcgagggcc ttcccggcgc gctggatggc cgtggccttg
      661 cccagcacgg ctatgctctg gaacagagga gggctgacct tggtgccggt taccgccacg
      721 cggagcggca tcaggaggtc gccgagcttg agtccggcct ccgcgccgcc cgagcggaag
      781 gccgcctcgc aggcgtcgtg gtcagccggg tcgttcgcct cgagcagctc catggccagt
      841 ccgagcgccc gcgcggcgcc ggcggcgtcc atcttcttgg gcagcagctc ctcgaccggc
      901 ggcacggccg gctcggcgaa caggaaccgc agggcttccg gcgcgtccga caggtacttg
      961 aggcgctcct tgaccagcgg cgccgccgcc agcaacctgg ctacctctag cgcgctcggc
     1021 ttggcggata cgagtccggc ctcggccatg aagggagtaa tcctcgcggc cagctcctcg
     1081 tcggacagca tgcggatgta ctggccgttg aaccactcga gcttgacgta gtcgaagacg
     1141 gccggcgcct tgttgatatg gtccatgctg aacaaccggg ccaggtcctc gagcgagtag
     1201 aggtcgcggc catcctcgta ggagcaaccg agcatggcca catagtttat cagcgccgcc
     1261 tcgaggtaac cgcgcctgcg gaactcgtcg acggccgtgg cgccgtggcg tttactgagc
     1321 ttatgtccgt cgtcgccgag caccatcggc aggtggcaga gccggggcgg ctcccagccg
     1381 aaggcctcgt acatgatcat gtggagagga gccgacggta tccactcctg ggcgcgcatc
     1441 acatgggtga tgcccatcag gtggtcgtcg acgacgttgg ccaggtgg
//
LOCUS       DAOOLN010000576         1486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_112055_length_1486_cov_3.510832, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000576 DAOOLN010000000
VERSION     DAOOLN010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112055_length_1486_cov_3.510832"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="PLW80_07755"
     CDS             complement(<1..1254)
                     /locus_tag="PLW80_07755"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPB66440.1"
                     /translation="MAARSFITKSRVYRQHLAVMAAGLLFSVAWGYVFAIVGPTGILK
                     AAYPLSAVNTLVAVLAASYAFSSTRVFQPRLVAKTFGVWLAIIVAFALPLGAVVAVAF
                     LFRAEAGATALGIAAIAFLLLGRGAESFARGRMGSEHDAASREDLEAAIAHLDLSAGR
                     ESVLGELSAIVGESFGCSWLAVLSEDDAGNLRRIYPDDDDEIVTTPNSPALEALAAVD
                     RRVILKTDVVADASFAELKPVLLGFFDVLGAEAIVIAKEGRRVVGLFALGPKRSGADY
                     DAQDYAAFDAVHGKLFVVAYYARHVARESLLGTVEKEIGLADQIVKSIQEKIDPIDHP
                     GVEVSFVCQSPRGLGGDLFDSVRISEHRWFFVVGDISGKGLNASMSMVILKSMIRTLL
                     KEEKDFIKLVARANVFIKERLPRGTF"
     gene            1253..>1486
                     /locus_tag="PLW80_07760"
     CDS             1253..>1486
                     /locus_tag="PLW80_07760"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPB66441.1"
                     /translation="MVKDEVTGLENLPAEPHVVLWKHSSAWETFSQFLIGPPKVLVLK
                     RELIFIPFFGWGLAQLRSIAIDRGAGASAVTQVV"
BASE COUNT          264 a          484 c          511 g          227 t
ORIGIN      
        1 gaaggtaccg cgcggcaggc gttccttgat aaagacgttc gccctcgcca ccagcttgat
       61 aaagtccttc tcctccttga gaagcgtgcg tatcatcgac ttgaggatga ccatcgacat
      121 gctggcattg aggcccttgc cggagatgtc gcccacgacg aagaaccagc gatgttccga
      181 gatgcgcacc gagtcgaaca agtcgccgcc gaggccgcgc ggcgactggc agacgaagga
      241 cacctcgacg cccgggtggt cgatggggtc gatcttctcc tggatcgact tgacgatctg
      301 gtcggctagg ccgatctcct tctccaccgt gccgagcagg gactcgcgcg ccacgtggcg
      361 ggcgtagtag gcgacgacga agagcttgcc gtgtaccgcg tcgaaggcgg cgtagtcctg
      421 ggcgtcgtag tcggcgccgg agcgcttcgg tccgagggcg aacaggccga cgacgcgacg
      481 gccttccttg gctatcacga tggcctcggc gccgagcacg tcgaagaaac cgagcagcac
      541 gggcttgagc tccgcgaaag aggcgtcagc caccacgtcc gtcttgagta tcacgcgcct
      601 atcgaccgca gcgagggcct cgagcgcggg ggaattcggg gtcgttacga tctcgtcgtc
      661 gtcgtccggg tagatgcgcc gcaggttgcc agcgtcatcc tcggacaaca cggcgagcca
      721 cgagcagccg aacgactcgc cgacgatggc cgaaagctct ccgagcaccg attctcggcc
      781 ggccgacagg tcgaggtgcg ctatagcggc ctcgaggtct tcccgggagg cggcgtcgtg
      841 ctccgagccc atcctaccgc gcgcgaacga ctcggcgccc ctgccgagga gcaggaaggc
      901 gatggcggcg atgcccaggg ccgtcgctcc ggcctcggcc ctgaacagga acgctacggc
      961 cacgaccgct ccgagcggta gcgcgaaggc gacgatgatg gccagccaca cgccgaaggt
     1021 cttggccacg agcctgggct ggaacacgcg agtcgacgag aaggcgtacg aggcggcgag
     1081 caccgccacg agcgtattga ccgcggacag cggatacgcc gccttgagga tgcccgtcgg
     1141 cccgacgatc gcgaagacgt agccccaggc gacgctgaac aggaggccgg cggccatgac
     1201 cgcgaggtgc tgcctgtaga cgcggctctt cgtgatgaac gagcgcgcgg ccatggtcaa
     1261 ggacgaggtc accggcctcg agaacctgcc ggccgagccg cacgtggtgc tgtggaagca
     1321 ctcctcggcc tgggagacgt tctcgcagtt cctgatcggc ccgccgaagg tgctggtgct
     1381 gaagcgcgaa ctgatcttca tcccgttctt cggctggggc ctcgcgcagt tgcgctcgat
     1441 cgcgatcgac cgcggcgccg gcgcctcggc ggtgacccag gtcgtc
//
LOCUS       DAOOLN010000577         1484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_112336_length_1484_cov_2.919524, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000577 DAOOLN010000000
VERSION     DAOOLN010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112336_length_1484_cov_2.919524"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..>1484
                     /locus_tag="PLW80_07765"
     CDS             29..>1484
                     /locus_tag="PLW80_07765"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB66442.1"
                     /translation="MDIMLAGDMDGVEAAKAVKASKDIPIIFLTAYTDERTLERAKEV
                     EPYGYILKPFKERELYTTIDIALYKHEMASKLTRQERLFSAILQSINDGIVALDTDLN
                     VSFMNQVAEDIVGTSEREAKGKHVGTILSILDPATKSDMLTGMINVGKRSTFFTDVSL
                     RNLAGQTVVLDGAITKIHEQANSTDGYLITMRDITELRRLSDSLNYQSSHDSLTGLSN
                     REEFSYKLSEILKNVKMVGGSHALLVLDIDRFKAVNDTCGTLAGDELLRQIASHVQTN
                     IQRNDLSARIGADEFAIILMNCELEDTINVGNRLQGAVQRQRFQWQATTFPITLSIGI
                     VPIDRDAGDIHAVLAAADDSCHISKEEGGNKITIFSAQDSKFKRRRGDMEWISKINRA
                     VEENRFLLYYQPIEPLGAGAEPKIEILLRLVNEDGSISGPTDFIPAAERYNLMPQVDR
                     WVLENSMRAFRLLSDQGSPLADRIFSINLSGPALL"
BASE COUNT          321 a          508 c          436 g          219 t
ORIGIN      
        1 gctcgagctc gcccccgaca tcattctgat ggacataatg ctggccggcg acatggacgg
       61 cgtcgaggcc gccaaggccg tcaaggcgtc caaggatatc ccgatcatct tcctgaccgc
      121 ctacaccgac gagaggacgc tcgagcgcgc caaggaagtg gagccgtacg gctatatcct
      181 gaagccgttc aaggagcgcg agctctatac gacgatagac atagccctct ataagcacga
      241 gatggccagc aagctgaccc gccaggagcg gctgttcagc gccatcctgc agtccatcaa
      301 cgacggcatc gtcgcgctgg acaccgacct gaacgtcagc ttcatgaacc aggtggccga
      361 ggacatcgtc gggacgagcg agcgcgaggc caagggcaag cacgtcggca ccatcctatc
      421 gatcctcgac ccggcgacga agagcgacat gctcaccggc atgatcaacg tcggcaagcg
      481 ctccaccttc ttcaccgacg tgtcgctcag gaacctggcg ggccagaccg tggtgctcga
      541 cggggcgatc acgaagatcc acgagcaggc caactcgacc gacggctacc tcatcacgat
      601 gcgcgacatc accgagctca ggcgcctatc ggactcgctc aactaccagt cgagccacga
      661 cagcctgacc ggcctgtcga accgcgagga attcagctac aagctcagcg agatactcaa
      721 gaacgtcaag atggtcggcg gctcgcacgc cctgctcgtg ctggacatcg accgcttcaa
      781 ggcggtcaac gacacctgcg gcaccctcgc cggcgacgag ctcctcaggc agatagccag
      841 ccacgtccag accaatatcc agcgcaacga cctctcggcg cgcataggcg ccgacgagtt
      901 cgccatcatc ctgatgaact gcgagctcga ggacacgatc aacgtgggca accgcctcca
      961 gggcgcggta cagcgccagc gcttccaatg gcaggcgacc acgtttccca tcaccctgag
     1021 catcggcatc gtgccgatcg accgcgacgc cggcgacatc cacgcggtgc tcgcggccgc
     1081 ggacgattcc tgccacatct ccaaggagga aggcggcaac aagataacga tcttcagcgc
     1141 ccaggacagc aagttcaagc ggcggcgcgg cgacatggag tggatcagca agatcaaccg
     1201 ggccgtcgag gagaaccgct tcctgctcta ctaccagccc atcgagccgc tcggcgcggg
     1261 cgccgagccg aagatcgaga tactactgcg cctggtcaac gaggacggca gcatctccgg
     1321 gcctaccgac ttcatcccgg cggccgagcg ctacaacctg atgcctcagg tcgaccgctg
     1381 ggtgctcgag aactcgatgc gggcgttcag gctattgtcc gaccaggggt cgccgctggc
     1441 cgaccggatc ttctcgatca acctctcggg cccggcgctg ctcg
//
LOCUS       DAOOLN010000578         1483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_112448_length_1483_cov_3.150560, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000578 DAOOLN010000000
VERSION     DAOOLN010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112448_length_1483_cov_3.150560"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PLW80_07770"
     CDS             complement(<1..897)
                     /locus_tag="PLW80_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HPB66443.1"
                     /translation="MALGCKYGTHRVIEPVGAMPQTAQRIDNDMTKVYDNEILLDVIA
                     LNVDSASFTQIEEEAGGDPDKIGKKIMEIVAARGKQQNPVTGSGGMFIGKVARIGSAL
                     KDRDLKVGDKIASLVSLSLTPLKINKILKVMPEVDRVLIDGQAILFESGIYAKLPPDM
                     DEGLALAALDVAGAPAQTAKLVKPGDSVLVLGAGGKSGLMCCYEAMKRVGPTGRVIAN
                     VRKEKYVKTLRDLKLCHEIVMADAQKPVEVYNEVLKANGGKEVDVVINCVNIQGTEMA
                     SILPVRDNGIVYFFSMATSFTRA"
     gene            complement(1043..1414)
                     /locus_tag="PLW80_07775"
     CDS             complement(1043..1414)
                     /locus_tag="PLW80_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66444.1"
                     /translation="MVTDEARLLKELIRKEERLLENSVRNDPAKVGALVDARGVFVSA
                     SGARGALEPGKPFDSLQGVSYIDSNTVELLDLSEDCRLLVYVAATVSKNTRAKSRCSS
                     VWKRDSGDWKVIFHQETTILD"
BASE COUNT          251 a          461 c          463 g          308 t
ORIGIN      
        1 ggcgcgggtg aagctggtgg ccatgctgaa gaagtagacg atgccgttgt cgcggacagg
       61 caggatgctc gccatctcgg tgccctggat gttgacgcag ttgatgacga cgtcgacttc
      121 cttgccgccg ttggccttga gcacctcgtt gtagacctcg accggtttct gggcgtcggc
      181 catcacgatc tcatggcaga gcttgaggtc gcggagcgtc ttaacgtact tttccttgcg
      241 gacgttggct atgaccctgc cggtcgggcc gacgcgcttc atggcctcgt agcagcacat
      301 caggccggac ttgccgccgg ctccgagcac gagtacgctg tcgccgggct tgaccagctt
      361 ggcggtctgg gcgggcgctc cggccacgtc gagagcggcc agggccagtc cctcgtccat
      421 gtccgggggg agcttggcat agatgcccga ctcgaagagg atggcctgtc cgtcgatcag
      481 gacgcggtcc acctcgggca tcaccttgag gatcttgttg atcttgagcg gggtcagcga
      541 cagcgacacg aggctggcga tcttgtcgcc gaccttgagg tcgcgatcct tgagcgccga
      601 gccgatcctg gccaccttgc cgatgaacat gccgccggac ccggtgaccg ggttctgctg
      661 cttgccgcgg gccgctacaa tctccatgat cttcttgccg atcttgtccg ggtcgccgcc
      721 ggcctcttcc tcgatctgcg tgaagctcgc ggagtcgacg ttgagcgcga tgacgtcgag
      781 caggatctcg ttgtcgtaga ccttggtcat atcgttgtcg atacgctgcg cggtctgcgg
      841 catggccccg accggttcga tgacgcggtg ggtgccgtac ttgcatccta aagccataat
      901 gcctccttct gggtagtttc cggcgcgatc cgagggcgac gtagccggat agatgtacgg
      961 atcatacctt ataaacccgc cccagaggaa gcgtcaagtt acgttaaaat agccgtttca
     1021 ccaggagcaa cgatggcctc gattagtcca gtatagtcgt ttcctgatgg aatatgacct
     1081 tccaatcgcc cgaatcgcgc ttccagaccg agctgcagcg cgacttggcg cgggtattct
     1141 tactgacggt agccgcaacg tagacaagca acctgcaatc ctctgatagg tccaggagct
     1201 cgaccgtgtt gctgtcgata tacgagacgc cctgcaggct gtcgaacggc ttgcccggct
     1261 ccaaggcgcc acgggcgcca gacgccgaga cgaacacgcc gcgcgcgtcg acgagcgcgc
     1321 cgaccttcgc cgggtcgttc cggacgctgt tctccagtag ccgctcttcc ttgcggatca
     1381 gctctttcag tagccgcgct tcatcagtga ccatcgcata cccccgggcg gcatcatagc
     1441 atggaaaacc ggcccccgtc ccgggggccg gcgagggtcg tca
//
LOCUS       DAOOLN010000579         1483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_112458_length_1483_cov_2.809524, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000579 DAOOLN010000000
VERSION     DAOOLN010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112458_length_1483_cov_2.809524"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="PLW80_07780"
     CDS             <1..1291
                     /locus_tag="PLW80_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB66445.1"
                     /translation="LEDFIATLPCAVLLVSHDRRMLDRVATSVLELETGGLTRYAGTY
                     SAYRLARLKQAAGQGQDWQADRKRIERLEALVRRFADIARSRPDPAWGKRLRAKRSQL
                     ERVRAEATERPDLGDRDARVSFLGEASKADLALRIDGYAKAYGDRVILEPSGFTVMNG
                     ERVALVGPNGSGKTSLVRDIVEFGSWDDERLRVGPSMVVGYCAQAQEVFDPGRTVEEE
                     FLKLLPTRKDALAHLGRFLFSWDDLDKRISSLSGGELNRLQLARASAMRANFLILDEP
                     TNHLDIPTREAVEDALADFDGTVLVVSHDRYFIEKVAERIVFIDRRRLVEYEGGFAEY
                     WRDVGSAGLSAGRRSTPRGLEGRGRSTTSSGASRGSGESEGLEQRIEAMEREKERLER
                     ASATAFDERDFKRAKAVAAELERHNAVLKKLWAEYVG"
BASE COUNT          256 a          471 c          515 g          241 t
ORIGIN      
        1 gctcgaggac ttcatcgcca cgttgccctg cgcggtgctg ctagtctccc atgaccggcg
       61 catgctcgac cgggtggcca cctcggtgct cgagctggaa accggcgggc tcacgcgcta
      121 cgccggtacc tattcggcct accgtctcgc gcgcctcaag caggcggcgg ggcaggggca
      181 ggactggcaa gccgaccgca agcgtataga acggctcgaa gccctcgttc ggcgcttcgc
      241 cgatatagcg cgctcgaggc cggacccggc ctggggcaag cgcctgcgcg ccaagcgtag
      301 ccagctcgaa cgcgtcaggg cggaggctac cgagcgtccc gacctaggag accgcgacgc
      361 ccgggtcagc ttcctcggcg aggcctcgaa ggccgacctg gccctgcgga tcgacggata
      421 cgcgaaggcc tacggggacc gcgtcatcct cgaaccgtcg ggctttaccg tgatgaacgg
      481 cgagcgggtc gccctggttg gcccgaacgg ctcgggaaag acgagcctcg tgcgcgacat
      541 cgtcgagttc ggatcctggg acgacgagcg cctgcgcgtc gggccgagca tggtggtcgg
      601 ctactgcgcc caggctcagg aggttttcga tcccggacgc accgtcgagg aggaattcct
      661 caagctgctg ccgacgagaa aggatgcgct ggctcacctc gggcgcttcc ttttctcatg
      721 ggacgacctg gataagcgca tttcgagcct gtccggcggc gagctcaacc gcctccagct
      781 ggctagggcc tcggcgatgc gggcgaattt ccttatatta gacgagccca caaaccacct
      841 cgatatcccg acgcgcgagg cggtggagga tgccctggct gattttgacg gcacggtgct
      901 tgtcgtgtct catgaccgtt atttcatcga gaaggtggcc gagcgtatcg tgttcatcga
      961 ccggaggcgc ctggtcgagt acgagggcgg cttcgccgaa tactggcgcg acgtgggctc
     1021 ggccggcttg agcgccgggc ggcgctccac tccccgcggg ctcgagggcc gaggcagatc
     1081 caccacctcg agcggcgcct cgcgcggttc gggcgagagc gagggcctcg agcagaggat
     1141 agaggctatg gagcgcgaga aggaacggct cgaacgcgcg tccgccacgg cgttcgacga
     1201 gcgcgacttc aagagggcca aggccgtagc cgctgagctc gagcgccaca acgccgtgct
     1261 caagaagctg tgggccgagt acgttgggtg atcgcctttg gcgacggctc ggtcgtagtg
     1321 taaggagagt caatgcatga atacaagctg gccgacgaga cgcccgtaga actcgtcgcc
     1381 aacggcgagc gcgtggccat cctcttgtgc acgccgatca acctcgacga cttggccgtg
     1441 gggcatctat tctcgcgcgg gatgctgcgc gacagggcca aag
//
LOCUS       DAOOLN010000580         1483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_112476_length_1483_cov_2.561625, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000580 DAOOLN010000000
VERSION     DAOOLN010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112476_length_1483_cov_2.561625"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..905)
                     /gene="speD"
                     /locus_tag="PLW80_07785"
     CDS             complement(105..905)
                     /gene="speD"
                     /locus_tag="PLW80_07785"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HPB66446.1"
                     /translation="MVRRIKGQTIKLDGFNNLTKSLSFNIYDICYARSTESQIEYLEY
                     IDEEYNSARLGTIVEEVTRIINAKLVQVSTQDYEPRGASVVALLNEDQYPAHTTPAPG
                     VLGHLDKSHIAIHTYPEYNQLSGIATFRVDVDIATCGMISPLSALHYLIESLESDVVI
                     IDYRVRGFTRDSRGVKLFIDHDISSIQDFIAEDALDDYTAYDVNIVSDNNFHTKMRRK
                     GLKLKDYLFGDEVTSLPPKEKRRIRQLLDLEVQEIFECKNLGRRMGRP"
     gene            1018..1365
                     /locus_tag="PLW80_07790"
     CDS             1018..1365
                     /locus_tag="PLW80_07790"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPB66447.1"
                     /translation="MSDMPDWVTGFPGAVTVSDRDHRIVYMNDKSAATFQKDGGRALV
                     GSSLMACHNERSKAIIDRLLAEGGQNVYTIDKAGQKKLIFQSAWRDEAGEVAGLVELS
                     LVLPADMPHYVRG"
BASE COUNT          255 a          434 c          501 g          293 t
ORIGIN      
        1 gcggccgatg tcgtagaaca tgccggcatg ggcgttgagg cgctcgcgca gctcgcgatg
       61 accgccggtc gccgcatcgc gcccgtcgtc acggcagttc gccgtcatgg gcgtcccatg
      121 cggcggccga ggttcttgca ttcgaagatc tcctgcacct cgaggtcgag gagctgcctg
      181 atgcgccgct tctccttggg cggcaggctc gtcacctcgt cgccgaacag gtaatccttg
      241 agcttgagac ccttccggcg catcttggta tggaagttgt tgtcggagac gatgttgacg
      301 tcgtaggccg tgtaatcgtc gagggcgtct tccgcgataa agtcctggat cgacgatata
      361 tcgtgatcga tgaacagctt gacgccgcgg ctgtcgcgcg tgaagccgcg gactcggtag
      421 tcgatgatga cgacatccga ctcgaggctc tcgatcaggt agtggagggc ggacagcggg
      481 ctgatcatgc cgcaggtggc tatgtcgacg tcgaccctga aggtggcgat gccggacagc
      541 tggttgtact cgggataggt atggatcgct atgtggctct tgtccaggtg gcccaggacg
      601 ccaggcgcgg gggtcgtgtg cgccggatac tggtcctcgt tgagcagggc gacgacgctg
      661 gccccgcgcg gctcgtagtc ctgggtcgag acctgcacga gcttggcgtt gatgatgcgc
      721 gtcacctcct cgacgatggt cccgagcctg gcggaattgt actcctcatc gatgtactcg
      781 aggtactcga tctgcgattc ggtcgacctg gcgtagcaga tgtcgtagat gttgaagctt
      841 aaagatttcg tcaggttatt gaacccgtcc agcttgatcg tttgtccctt gattctccgt
      901 accaatgtgc ccgtgccccc tcgaatctca tggtgatgag cgaacggcca ttgtaacccg
      961 gcctggcgct ttggtacagt tgccgggcgc cggcaatgcg gcgagcgagg agcgaatatg
     1021 agcgacatgc ccgattgggt aaccgggttc cccggcgcgg tgacggtatc ggaccgcgat
     1081 cacaggatag tctacatgaa cgacaagtcg gcggcgacgt tccagaagga cggcgggcgg
     1141 gcgctcgtcg gctcgagcct gatggcctgc cataacgagc gatcgaaggc catcatcgac
     1201 cgcctgttgg ctgagggcgg gcagaacgtc tacacgatag acaaggcggg tcagaagaag
     1261 ctgatcttcc agagcgcgtg gcgggacgaa gccggagagg tcgccggcct cgtcgaactg
     1321 tcgctcgtcc tgccggccga tatgccccac tacgtgcgcg gctgagacgc cggcagcgcc
     1381 gtggcgacct cccaggtctc gttgccgccg gatgggttcc cgacgcggcc gaagaccagc
     1441 acgaaccgcc cgcgcggtat ctccgcgaac cggccgcgta gct
//
LOCUS       DAOOLN010000581         1482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_112591_length_1482_cov_2.419762, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000581 DAOOLN010000000
VERSION     DAOOLN010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112591_length_1482_cov_2.419762"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PLW80_07795"
     CDS             <1..759
                     /locus_tag="PLW80_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPB66448.1"
                     /translation="YFVRCRDRAAYLSAIADEAASYAPGTTVEYSDLGFMLLAWILER
                     VWDEALPALVDRLVLSPLGMGLTGYPAAARLRFADAAFVPTEAGNEYERSMTLAYAEG
                     RPVVGGPAGRYRIGVAELDGVPWRRGVILGEVHDGNCHYGLGGVSGHAGLFSTAEDLV
                     RFMAFWGPSGPLGPALRAEAFRRQTPDGASPRGLGWILDGPAATHTGFTGTMLRYRPG
                     DGRALVALTNRVYPSVKDGIGAWRQAFVATLEAV"
     gene            756..1292
                     /locus_tag="PLW80_07800"
     CDS             756..1292
                     /locus_tag="PLW80_07800"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB66449.1"
                     /translation="MTGDVAIRALRADEAEPFLALRKAADAQSRYLLYGAGERRTTVE
                     EQARAIAELLGSGNSTILVAEAEGRDGAGSLVGYLIAKGGGAERNRHAAEIVVAILDA
                     YTGKGIGARLFGALDEWARGAGVTRLGLGLMDGNVRARALYEKMGFREEGRRKAAFLL
                     EGGFVDEILMYKLPSSIL"
     gene            1302..>1482
                     /locus_tag="PLW80_07805"
     CDS             1302..>1482
                     /locus_tag="PLW80_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66450.1"
                     /translation="MKKRVLIAAALCAMALSLTFGQAPKRGGFLRMTPAKQGVLVQNF
                     NPFSPAALESTVGCFY"
BASE COUNT          221 a          491 c          539 g          231 t
ORIGIN      
        1 tatttcgtgc gctgccggga ccgggcggcg tacctctcgg ccatcgcgga cgaggcggcg
       61 tcctacgcgc ccggaacgac ggtcgagtac agcgacctcg gcttcatgct gctggcttgg
      121 atactcgagc gcgtatggga cgaggcgctg cccgccctgg tcgatcggct cgtgctgagc
      181 ccgctcggca tgggcctgac aggctatcct gcggccgcgc ggctgcgctt cgcagacgcg
      241 gcgttcgtcc ccacggaggc cggcaacgag tacgagcggt cgatgaccct ggcctacgcc
      301 gagggccgcc cggtggtcgg cggcccggcc ggtaggtatc gaatcggcgt cgccgagctg
      361 gacggcgtac cctggcggcg cggcgtcatc ctcggcgagg tccacgacgg caactgccat
      421 tacggtctgg gcggcgtcag cgggcacgcc ggcctgttct ccacggcgga ggacctggtc
      481 cgcttcatgg ccttctgggg cccctcaggt ccgctcggcc cggccctgag ggcggaggcc
      541 ttccgtcgcc agacgccaga cggcgcctcc ccgcgagggc tcggctggat actcgacggg
      601 ccggcggcca cgcatacggg ctttaccggt accatgctac gctatcggcc gggcgacggc
      661 cgcgcgctcg tcgcgctgac caaccgcgtg tatccgtccg tcaaggacgg cataggcgcc
      721 tggcgccagg ccttcgtcgc gacgctggag gcggtatgac cggcgatgtc gcgatacggg
      781 ccctgagggc ggacgaggcc gagcccttcc tggcgctgcg caaggccgcc gacgcccaga
      841 gccgctacct gctctacgga gccggagagc gcaggaccac ggtcgaggag caggctaggg
      901 ctatcgcgga gctgctcggc tcggggaact cgaccatcct ggtcgccgag gccgagggcc
      961 gcgacggcgc cggaagcctc gtcggctacc tgatcgccaa gggcggcggc gccgaacgca
     1021 ataggcacgc ggcggagata gtcgtggcca tattggacgc gtataccggc aagggcatag
     1081 gggcccgatt gttcggggcg ctcgacgagt gggcccgcgg ggccggcgtg accaggctcg
     1141 ggctgggcct gatggacggc aacgtccggg ccagggcgct ctacgaaaag atgggatttc
     1201 gcgaggaggg ccgcaggaaa gccgctttcc tccttgaggg cggcttcgtg gatgagatcc
     1261 tcatgtacaa gctgccaagc tctattttat aggaggatgg aatgaagaaa cgcgttctta
     1321 tcgccgcggc gctctgcgcc atggcgctct cgctcacgtt cggccaggcg ccgaagcgag
     1381 gcggcttcct caggatgacg cccgcgaagc agggcgtgct ggtgcagaac ttcaatccct
     1441 tcagcccggc cgccctcgag tcaacggtag gctgcttcta cg
//
LOCUS       DAOOLN010000582         1481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_112657_length_1481_cov_3.328892, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000582 DAOOLN010000000
VERSION     DAOOLN010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112657_length_1481_cov_3.328892"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1481)
                     /locus_tag="PLW80_07810"
     CDS             complement(<1..>1481)
                     /locus_tag="PLW80_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66451.1"
                     /translation="RDSLYEEINELRAENLALAQAADEAEYYRLRTGELESELERLEA
                     ELAAKEAELAAKEAELADADARLDAIGAQLAEEGARLADAEARLALVELDDDALGAAL
                     IEKDALLAEKDALLSEKDASLAEKDATIAEKDASLAEKDALLVERDASVAELDARIAS
                     LDSELAEARAALAEAESKLYESTERAAPVATVAPTAAPVRDDGGEASAATIAALEARV
                     AELSAERDAAVATRDAAVATRDASDAMRLATERILDETKAELERALADAKASGAIVAA
                     PVPAATPGPSTVARPAPSGGYLSGWAIDSSRFTRSLRSGFDGSVARMGAWKLAGQVAS
                     QTDPKQYFSRLEMPLAQGQAATLYRFKARSTGDGWVGVGLHLYVEDVKKRHGYGEGRS
                     LLVWFTRDRAARGDDATYLQLYRSDDDVVMERMFDAELSDGIEAWRTVEVLYDPAAEF
                     IAVSVDGVLRIVYKTFFGRDSGATVSLRTLGAGGSFSDFSAWA"
BASE COUNT          196 a          529 c          511 g          245 t
ORIGIN      
        1 cggcccaggc ggagaagtcg gagaacgatc cgccggcgcc gagggtccgt agcgacacgg
       61 tggcgcccga gtcacggccg aagaaggtct tgtagacgat gcgcaggacg ccatcgacgg
      121 agacggcgat gaattcggca gcggggtcgt agagtacctc gaccgtgcgc caagcctcga
      181 tgccgtcgga cagttccgcg tcgaacatgc gctccatcac cacgtcgtcg tcggatcggt
      241 acagttgcag gtaggtggcg tcgtcgccgc gggccgcccg gtcgcgcgtg aaccacacga
      301 gaaggctccg cccctcgccg tagccgtggc gcttcttcac gtcctcgaca tagaggtgca
      361 gaccgacgcc gacccagccg tcgccggtcg agcgcgcctt gaagcggtac agcgtggcgg
      421 cctggccctg ggccagcggc atctccagcc gggagaagta ctgcttcggg tcggtctggc
      481 tcgcgacctg gccggctagc ttccaggccc ccatccgcgc caccgagccg tcgaagcccg
      541 agcgcagcga tctggtaaag cgagacgagt cgatggccca gccggacagg tagccgccgg
      601 acggcgctgg cctcgctacg gtggacggcc caggcgtcgc ggccgggacc ggagctgcta
      661 cgatggctcc gctcgccttg gcgtcggcca gggcccgctc gagctcggcc ttcgtttcat
      721 cgaggatgcg ctcggtggcc aggcgcatcg cgtcggaggc gtcgcgcgtg gcgaccgccg
      781 cgtccctcgt cgctacggcc gcgtcgcgct cggcgctcag ctcggccacc ctcgcctcca
      841 gggcggctat cgtcgcggcc gaggcctcac cgccgtcgtc acgaacgggg gcggccgtgg
      901 gcgctacggt tgcgacgggc gccgcccgct cggtcgactc gtaaagcttt gactcggcct
      961 cggccagcgc ggccctcgcc tcggcgagct cggagtcgag cgaggcgatg cgcgcgtcca
     1021 gctcggcgac gctcgcgtcc cgttcgacga gcagggcgtc cttctcggcc agcgacgcgt
     1081 ctttttcagc tatcgtcgcg tccttctccg cgagcgaggc gtccttctcg gatagcaacg
     1141 cgtctttctc ggccagtagc gcgtccttct cgatcagcgc ggcaccgagc gcgtcgtcgt
     1201 ccagctcgac gagggccagg cgggcctcgg cgtcggccaa cctggcgccc tcttcggcta
     1261 attgggcccc gatggcgtcc aatcgggcgt ccgcatcggc caattcggct tccttggcgg
     1321 ccagctcggc ttccttggcg gccagctcgg cttcaagccg ctctagctcc gactccagct
     1381 ctcccgtgcg cagtctatag tactcggcct cgtcggccgc ctgggcgagc gctaggttct
     1441 cggcgcgtag ctcgttgatc tcctcgtaga gggagtcgcg c
//
LOCUS       DAOOLN010000583         1481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_112663_length_1481_cov_3.061711, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000583 DAOOLN010000000
VERSION     DAOOLN010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112663_length_1481_cov_3.061711"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /locus_tag="PLW80_07815"
     CDS             complement(<1..1420)
                     /locus_tag="PLW80_07815"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HPB66452.1"
                     /translation="MKASMTDRIARLRHESVEAKPSIDIQRALLITEAYTSMPDGLPA
                     PIVRARSFAYLCEHKDIYIGANDLIVGERGRGPKRTSTYPELTCHSVEDLVTLRSRPM
                     TSYDISDDDIESYRERVIPYWRGRCVRDRAFAGLPAEWLALYEAGVFTEFMEQRAAGH
                     TALDGLLYRKGLRELKSEAAAARSALSPEDPRLEEKVAELEAMATSCDAMIRLAERHA
                     DRAEAMAASERNAARALELRDIAAVCRRVPAEAPRTFHEALQAYWFVHLGTLTELNGW
                     DAMSPGHLDQHLEPFYLRDLADGRLTRERAKELLSAFWIKVNNTPAPPKVGITAAESG
                     TYNDFTNINLGGLAPNGRDGSGEVTLLALEVLEELRLLQPQANLQVSSRTPDRVLEAA
                     CAVVREGMGYPSLFNADEVTLAQVASGKSLRDAREGGTSGCIETGCFGKEAYILHGYL
                     NGPKLLELALNDGIDPASGRRVG"
BASE COUNT          233 a          450 c          512 g          286 t
ORIGIN      
        1 acccgacgcg tcggccgctg gccgggtcga taccgtcatt cagggccagc tcgaggagct
       61 tgggcccgtt caggtagccg tggagaatgt aggcttcctt gccgaagcag ccggtctcga
      121 tgcagcctga ggtgccgccc tcgcgcgcgt cgcgcaggct cttcccggag gcgacctggg
      181 ccagcgtcac ctcgtcggcg ttgaacagcg acggatagcc catgccctcg cggacgacgg
      241 cgcaggcggc ctcgagcacg cgatcgggcg tcctggacga gacctgcagg ttcgcctgcg
      301 gttgcagtag cctgagttcc tcaagcacct cgagggccag gagcgtcacc tcgccggagc
      361 cgtcgcggcc gttgggcgcg aggccgccga ggttgatgtt ggtaaagtcg ttataggtgc
      421 ccgactctgc ggcggtgatg ccgacctttg gtggggctgg agtgttgttc accttgatcc
      481 agaaggcgga caggagctct ttggcccgct cgcgggtcag gcggccgtcg gcgaggtcgc
      541 gcaggtagaa cggttcgagg tgctggtcga gatggcccgg actcatggcg tcccagccgt
      601 tgagctcggt cagcgtgcca agatgcacga accaataggc ctgtagcgcc tcatggaagg
      661 tcctcggggc ctcggccggt actctgcggc ataccgcggc tatgtcccgt agctcgagcg
      721 cgcgggcggc gttccgttcc gaggcggcca tcgcctcggc ccgatcggcg tggcgctcgg
      781 ccagcctgat catagcgtcg caggaggtgg ccatggcctc gagttcggcc accttttcct
      841 cgagccttgg atcctcgggc gatagagccg aacgggccgc cgcggcttcg gacttgagtt
      901 ctcgcaggcc cttgcggtag aggagtccgt cgagcgcggt atggccggcg gctcgctgtt
      961 ccatgaactc ggtaaagacg cccgcctcgt agagggcgag ccactcggcc ggaaggcctg
     1021 cgaaggcgcg gtcacggacg cagcggccgc gccagtaggg gatgacccgt tcgcggtacg
     1081 actcgatatc atcgtcgcta atatcgtagc tggtcatcgg cctcgaacgt agcgtcacta
     1141 ggtcttcgac gctgtggcag gttaattcgg gataggtcga ggtcctctta gggccgcgcc
     1201 cgcgttcgcc gacgattaga tcgttggctc cgatgtagat gtccttatgc tcgcagaggt
     1261 aggcgaagga acgcgcccgg acgattgggg cgggtaggcc gtcgggcatc gaggtgtagg
     1321 cttcggtgat caagagagca cgctggatat cgatgctcgg cttcgcctcg acgctctcgt
     1381 gtcgcaatcg cgcaatccta tcggtcatac tcgccttcat gccgtatcgt catgtattca
     1441 atatagtaca caagcgcatt gttcggcctg tcctacggca a
//
LOCUS       DAOOLN010000584         1479 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_112878_length_1479_cov_3.714888, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000584 DAOOLN010000000
VERSION     DAOOLN010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112878_length_1479_cov_3.714888"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="PLW80_07820"
     CDS             <1..631
                     /locus_tag="PLW80_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPB66453.1"
                     /translation="VTAFRIGLEGAQGYFGVRPDLTTFGKIVAGGYPSAGGLGGRKDL
                     ILMMAAGLESGKKRAYVGGTMAANPLSAAAGYFTIKEIEKRDACAVAGRAGDRLTAGL
                     KKLIAERGLPFVAYNQGSICHLETVGAMFIKMDYLKIVRVLKEIKIRKKMMETYGAAY
                     AAEGIVTLAGSRMYTSGADTDAVIDDALARFGRVFDNVDVEAMNLGAAE"
     gene            628..1281
                     /locus_tag="PLW80_07825"
     CDS             628..1281
                     /locus_tag="PLW80_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPB66454.1"
                     /translation="MTEAEAREAVRAAGVRLVEEGLVAGTWGNVSLRFSSDEIAITPT
                     GTDYMAMKVEDIVIVSLRTGEARGGKPSSELHMHEAVYASRPEIRAIVHTHSMSASTV
                     AAARREVPPVLDDLAQIVGPTLRVADYSLPGSKKMGRAVVRAMAGRMAALMANHGLVA
                     CGRNLQEALLCAQIAEKGCRAFVEAEFLGGAKSIGRFEAAVMHQVYLKKYSKGKGER"
     gene            1278..>1479
                     /locus_tag="PLW80_07830"
     CDS             1278..>1479
                     /locus_tag="PLW80_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66455.1"
                     /translation="MKGDELFVVAYDIGTTGAKTCLYRFDRAGVALAAAASSEYGIRF
                     LPNGGAEQNPDDWWKAICVGTRE"
BASE COUNT          271 a          486 c          506 g          216 t
ORIGIN      
        1 ggtcaccgcg ttccgcatcg gcctcgaggg cgcccagggc tacttcggcg tacggcccga
       61 cctgaccacc ttcggcaaga tagtcgccgg cggctacccg tcggcgggtg gccttggcgg
      121 gcgcaaggac ctgatcctga tgatggccgc gggcctcgag agcggcaaga agcgcgctta
      181 cgtcggcggc accatggccg cgaaccccct atccgcggcg gccggctact ttactatcaa
      241 ggagatcgag aagcgtgacg cctgcgccgt agccgggcgt gccggcgatc ggctcacggc
      301 cggcctcaag aagctgatag ccgaacgcgg cctgcccttc gtcgcctaca accagggctc
      361 catctgccac ctcgagacgg tcggcgcgat gttcatcaag atggactacc ttaagatcgt
      421 gcgcgtcctc aaggaaatta agatccgcaa gaagatgatg gagacctacg gcgcggccta
      481 cgccgccgag ggcatcgtga cgctcgcggg tagccgcatg tacacgagcg gcgcggatac
      541 cgacgccgtg atcgacgacg cgctcgccag gttcggccgg gtcttcgaca acgtcgacgt
      601 cgaggcgatg aacctcgggg ccgcggaatg accgaggccg aggcgaggga ggcggtccgc
      661 gccgcgggcg tcagattagt cgaagagggc cttgtcgcgg gcacctgggg caacgtatcc
      721 ctgcgcttct cgtcggacga gatagccatc acgccgaccg gcaccgacta catggccatg
      781 aaggtcgagg atatcgtcat cgtgagcctg cgtaccggcg aagcccgcgg cggcaagccg
      841 tcgagcgagc tccatatgca cgaggccgtc tacgcctcga ggcccgagat acgggccatc
      901 gtccacacgc attcgatgag cgcgtccacc gtcgcggcgg ccaggcgcga ggtgccgccg
      961 gtgctcgacg acctggcgca gatcgtaggc ccgacgctac gcgtggccga ctactcgctg
     1021 ccgggctcca agaaaatggg cagggccgtg gtcagggcga tggccgggcg catggcggcg
     1081 ctgatggcaa atcacggcct cgtcgcctgc ggccgcaacc tacaagaagc gctactctgc
     1141 gcccagatcg ccgagaaagg ttgccgcgcc ttcgtcgagg cggagttcct cggcggggcc
     1201 aagagcatcg gccgcttcga agccgcggtg atgcaccagg tctacctgaa gaaatactcg
     1261 aaaggcaagg gcgagcgatg aagggcgacg aactgttcgt cgtcgcctac gacataggca
     1321 ccaccggcgc caagacctgc ctctaccgct tcgaccgcgc cggcgtcgcg ctggcggcgg
     1381 ccgcgtcgtc ggagtacggt atacgcttcc tgccgaatgg cggcgccgag cagaatcccg
     1441 acgactggtg gaaagccatc tgcgtcggca cgcgcgagg
//
LOCUS       DAOOLN010000585         1476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_113228_length_1476_cov_3.038705, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000585 DAOOLN010000000
VERSION     DAOOLN010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113228_length_1476_cov_3.038705"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PLW80_07835"
     CDS             <1..314
                     /locus_tag="PLW80_07835"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB66456.1"
                     /translation="RAGAGTLWEGAASGKPMVFIPLGSGSRGDQVENARLLASRGGAV
                     CLAGDEATPEALLAALTPLLSNPEARSTMAAAAGETARPDAAAAIAGMILDRVLKGST
                     R"
     gene            311..790
                     /locus_tag="PLW80_07840"
     CDS             311..790
                     /locus_tag="PLW80_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPB66457.1"
                     /translation="MNVLDYVLLAVIAIAALRCWFRGIVGEVLSMAAVVGGLLAGIFF
                     FRPVGAWISNLKPLGGFEVVVGFVASFAVVFIVVKIVEKSLRSVLENLNLDILDSVLG
                     LIFGVVEGVIISAIVVMILHYQPVFDVEALLDGSWIARLLLPLVVEALPAARSSISG"
     gene            796..>1476
                     /locus_tag="PLW80_07845"
     CDS             796..>1476
                     /locus_tag="PLW80_07845"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66458.1"
                     /translation="MFENIMWQPNATEALKAELDRGALPGSLLFEGPPLSAKLSSALE
                     LSRALSCERDAAWNCPCQQCSRHRTLSHQDTLLLGPKSFREELAIGAAMMKRAPGSGS
                     RFFFTRAVKKLTRRFDQELYAGEEARLSKALPLVRSALEGVDACAPASPTGRSSALSD
                     DEAVKEAGKLLGVCAKLEDLLPAATPVFQVRAMEFWARLAPYGRRKTIIIEHADRMLD
                     ASRNALLKI"
BASE COUNT          239 a          502 c          470 g          265 t
ORIGIN      
        1 gcagggccgg cgccggtacg ctctgggaag gcgcggcctc gggcaagccg atggtcttca
       61 taccgctcgg ttcgggtagc cgcggcgacc aggtcgagaa cgcgcgcctg ctcgcgtcgc
      121 gcggcggggc tgtctgcctg gcgggcgacg aggcgacgcc cgaggccctg ctcgccgcgc
      181 tgacgccact cctgtccaat ccggaggcca ggtcgacgat ggccgccgcc gccggcgaga
      241 ccgccaggcc ggatgccgcg gccgccatag ccgggatgat actcgatcga gtactgaaag
      301 gaagcacgag atgaacgttc tagactacgt attgctggcc gtcatcgcca tcgccgcgct
      361 tcgttgctgg ttcaggggca tcgtcggaga ggtactgtcg atggcggctg tcgtgggcgg
      421 cctcctagcc ggtattttct tcttccgacc agtcggcgcc tggatatcga acctgaagcc
      481 gctcggcggg ttcgaggtcg tcgtcggctt cgtggccagc ttcgccgtcg tattcatcgt
      541 cgtcaagatc gtcgagaaat cgctccgtag cgtactcgag aacctgaacc tcgacatact
      601 cgacagcgtg ctcggcctta tcttcggcgt cgtcgaaggc gtgatcatct cggccatcgt
      661 cgttatgatt ctccattacc aaccggtctt cgacgtcgaa gccctgctcg acggaagctg
      721 gatcgccagg ctcctcctcc cgctcgtcgt cgaggcgcta cccgccgccc gctcgtcgat
      781 ctccggctga gccctatgtt cgagaacatc atgtggcagc ctaacgcgac cgaggccctc
      841 aaggccgagc tggaccgggg agccctacca ggctcgctcc tgttcgaggg cccgcccctg
      901 tcggcgaagc tcagctccgc cctcgagctg tccagggctc tgtcctgcga gcgcgatgcc
      961 gcctggaact gcccctgcca gcagtgctca cggcacagga cgctatcgca ccaggatacg
     1021 ctgttgctcg gccccaagtc cttcagggag gaactcgcca taggcgcggc catgatgaag
     1081 cgcgcccccg gctcgggctc gaggttcttc tttacccgtg cggtcaagaa gctgacgagg
     1141 cgattcgacc aggagctata cgccggcgag gaagcgaggc tctccaaggc cctaccgctg
     1201 gtgcgttccg ctctcgaggg cgtcgacgcc tgcgcgccgg cttccccgac gggccgctcg
     1261 agcgccctat ccgatgacga ggccgtcaag gaagcgggta agctgctcgg cgtctgcgcc
     1321 aagctcgagg acctgctccc ggcagccacg ccggtttttc aggttcgagc catggaattc
     1381 tgggccaggc tcgcgcccta cggcaggcgc aagaccatca taatcgagca cgcggaccgc
     1441 atgctcgacg cctcaaggaa cgcgctgctc aaaata
//
LOCUS       DAOOLN010000586         1475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_113364_length_1475_cov_2.721127, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000586 DAOOLN010000000
VERSION     DAOOLN010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113364_length_1475_cov_2.721127"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PLW80_07850"
     CDS             complement(<1..264)
                     /locus_tag="PLW80_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66459.1"
                     /translation="MSSHSILPESRRAAIEAEIDDALSEVRATADVALLQECRAIFRA
                     RVPFNLRAYVAATLALKYAGGSRHPETRSRDAKGGKGRSEGRPD"
     gene            357..818
                     /locus_tag="PLW80_07855"
     CDS             357..818
                     /locus_tag="PLW80_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66460.1"
                     /translation="MRRTTPLIAIALLVSVAAASGQAIVAPDDPATLLGLTPAQAIER
                     FGPPGSVFAIRGAEPWQDDVVFDYGGGFSLFLFKDRVWQVRIAEPYARPVLGLVVGSG
                     VERAISALGAPALALGGSYEWVLPGEAWPVRLRGVTDGGRIRELYIYRADF"
     gene            827..>1475
                     /locus_tag="PLW80_07860"
     CDS             827..>1475
                     /locus_tag="PLW80_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HPB66461.1"
                     /translation="MPNICLCLTGSTIAQDLEALERYRDKVDMVELRADYLDPAELFH
                     LRFFPEKAGMPSILTVRRKSDGGKFDLGEGVRLVTFARGLAFADSDHRKNFSYVDIEH
                     DFHIPSIQEAARTFGTKIIRSKHCLDGMPADIDKVWRELSSRPSEIPKLAVKANGMAD
                     VDSVFRLFEGGQGRGERVVVSMGEYGFCTRVLADRLGSMLVYASATGAGLEAPAPG"
BASE COUNT          242 a          486 c          491 g          256 t
ORIGIN      
        1 gtcgggcctg ccctcggacc gccccttacc gcccttggcg tcgcgggagc gcgtctcggg
       61 gtgacgggag ccgccggcgt acttgagcgc cagggtcgcg gcgacgtagg cccgtagatt
      121 gaacggcacc cgtgcgcgga agatggcccg gcattcctgg agcagcgcga cgtccgcagt
      181 agcgcgaacc tcggagagcg catcgtcgat ctcggcctcg atggccgccc gacgggattc
      241 tgggagtatc gaatgagatg acacgtatga ctccttagac gtaaacataa tcgaaaggcc
      301 cctactttct caacccaggc ttgcgacgcg tctccggctc cgatacagta tcggggatgc
      361 gccgaacaac gcccctcata gcgatcgccc tcctcgtatc cgtcgcggcc gcctccggtc
      421 aggcgatagt cgccccggac gatcccgcca cgctactggg cctgacgccg gcccaggcca
      481 tcgaacgctt cgggccgccc gggagcgtat tcgcgatacg cggcgccgag ccctggcagg
      541 acgatgtcgt cttcgactac ggcggcggat tctcgctatt cctcttcaag gatagggtct
      601 ggcaggtcag gatcgccgag ccgtacgcca ggccggtcct cggcctcgtc gtcggctccg
      661 gcgtcgaacg ggcgatctcc gcgctcgggg ccccggccct cgcgttgggc gggtcgtacg
      721 aatgggtctt gccgggagag gcctggccgg ttaggctcag gggcgtgacc gacggaggac
      781 gtatccgcga gctttatatc taccgagctg acttctagga gcggcgatgc cgaatatttg
      841 cctgtgcctg acgggatcga ctatcgccca ggacctggag gcgctggaac gctaccgcga
      901 caaggtggac atggtcgagc tacgcgccga ctacctcgac ccggccgagc tgttccacct
      961 gcgcttcttc ccggagaagg ccggcatgcc gagcatcctc acggtcaggc gcaaatccga
     1021 cggcggcaag ttcgaccttg gagagggcgt acggctggtc acgttcgcca ggggactcgc
     1081 gttcgccgac tcggaccatc gcaagaattt ctcgtacgtc gacatcgaac acgattttca
     1141 tatcccctcg atccaggaag cggcgcgtac cttcggcacc aagatcatcc gctcgaagca
     1201 ctgcctcgac ggtatgcctg ccgatatcga caaggtctgg cgggagctgt cgtctcggcc
     1261 gtcggagatt ccgaagctgg cggtcaaggc gaacggcatg gccgacgtcg actcggtttt
     1321 caggcttttc gagggcggcc agggccgcgg cgagcgggtc gtcgtcagca tgggcgagta
     1381 cggcttctgc acgcgggtgc tcgccgaccg cctcggcagc atgctcgtct acgcgagcgc
     1441 caccggggca gggctcgagg cgccggcgcc aggcc
//
LOCUS       DAOOLN010000587         1472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_113739_length_1472_cov_2.886380, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000587 DAOOLN010000000
VERSION     DAOOLN010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113739_length_1472_cov_2.886380"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="PLW80_07865"
     CDS             complement(<1..1145)
                     /locus_tag="PLW80_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66462.1"
                     /translation="MARDIDVYQLLKTFASRTKSDVLDYTAFAQAIQRQAKAYDQTEP
                     LYRDLSLHPDAVLVPRLFQLAREGRLSLQAVQNRVDLIYLPEAFTETLLAEYRRIEEN
                     PDIPFPDEDSMRLSVPPEWIQAVSVEADLPSLVAFEGDRPVPIYRLVFPEGLKPVVIL
                     SVLVGDKLLEYAILKLRNYLRKGSNRDFIQQRLAGAFSGKDRMLKDALTSILIKPFDS
                     LQAMKQGAGDFSYSFWAYLTTAVRKDLSGKSELMPDDIAAYQASYVVDVFNNHYKNLA
                     QRGQERESAFKSLSVMLRKPPYLYSIDQIVDFRDAQGRPLLGKYSREELEAWIQERTT
                     KAAEGALPELLILGSTGGSSGQVIAKETMIPYLVKALRDARASIKTSV"
BASE COUNT          250 a          447 c          481 g          294 t
ORIGIN      
        1 acggacgtct tgatcgaggc cctcgcgtcg cgcagggcct tgaccaggta gggtatcatc
       61 gtctccttgg ctatgacctg gccgctcgag ccgcccgtcg agccgaggat caacagctcc
      121 ggcagcgcgc cctcggccgc cttggtcgtc cgctcctgga tccaggcctc gagctcctcc
      181 cggctgtact tgccgagcag gggccggccc tgcgcgtccc tgaagtcgac gatctggtcg
      241 atcgagtaca ggtagggcgg ctttcggagc atgaccgaca gcgacttgaa ggccgactcg
      301 cgttcctggc cgcgctgggc caggttcttg taatggttat tgaacacgtc gacgacgtag
      361 gacgcctggt aggcggcgat gtcgtcgggc atgagctcgc tcttgcccga caggtccttg
      421 cgtacggccg tggtcaggta ggcccagaac gagtacgaga agtccccggc gccctgcttc
      481 atcgcctgca gcgagtcgaa gggcttgatc aggatgctcg tcagcgcgtc cttgagcatg
      541 cgatccttgc cggagaaggc cccggccagc cgctgctgga tgaagtcgcg gttcgagccc
      601 ttgcggaggt agttgcgtag cttcaggatc gcgtactcga gtagcttatc gccgaccagt
      661 accgatagga tcacgacggg cttgagcccc tcgggaaaca ccaatcggta gatcggcacg
      721 ggccggtcgc cctcgaaggc gaccagggac ggcaggtcgg cctcgaccga taccgcctgt
      781 atccattcgg gaggcaccga caggcgcatc gagtcctcgt cggggaaggg tatgtccggg
      841 ttctcctcga tgcggcggta ctcggctaac agcgtctcgg taaaggcctc ggggaggtag
      901 atcaggtcga cgcggttctg gacggcctgc agggacagcc gcccctccct ggccaactgg
      961 aagagcctgg gtacgagtac agcgtccgga tgcagggaca ggtcgcggta gagcggctcc
     1021 gtctgatcat aggccttggc ctggcgctgg atggcctggg cgaacgcggt atagtccagg
     1081 acgtcgctct tcgtcctgga ggcgaaggtc ttgaggagct gataaacgtc tatatcacgt
     1141 gccatagcaa ccattctatg tacggctcgc cgttcgggca ataaaaaacg gccgagccct
     1201 gcgggccgcg gccgttccgg tgcgatagcg cacggtcact tgcgggtctt atgccgattc
     1261 tttctcttct tcttcttccg cttgtgcgtg gctatcttct tgagcttgcg ttttcttccg
     1321 cagggcacgg tggttctcct tatggacccg agcgccgccc gagggcgaag cgatcgagta
     1381 cgcttagctt acgaagaggc tccggccatt atgcacgccg ccgcccggaa cgtcaatagc
     1441 ggcgccggtc atcatcgatt caaaatggac ag
//
LOCUS       DAOOLN010000588         1472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_113794_length_1472_cov_2.010586, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000588 DAOOLN010000000
VERSION     DAOOLN010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113794_length_1472_cov_2.010586"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..562
                     /locus_tag="PLW80_07870"
     CDS             59..562
                     /locus_tag="PLW80_07870"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HPB66463.1"
                     /translation="MEKVGLPDNLISSLVPGLSGQRADKKKEAAKASPKALRFRSALK
                     EAEEIDLTPAAEGLPFSEAEAAELLDSVHAAGEELKRDPDPACVKAYKSAVRDFVHYV
                     VGRAYDVVEKSSGGNILKRKKFTTVVVIDEKLERLAAEVLSAQRDKLDILRRLDEIHG
                     LLVDLLR"
     gene            595..>1472
                     /gene="ricT"
                     /locus_tag="PLW80_07875"
     CDS             595..>1472
                     /gene="ricT"
                     /locus_tag="PLW80_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HPB66464.1"
                     /translation="MDDDMDYMQGDEDLSHLEDDAVPAADAGVHCGELPDPLYRLRAD
                     PYNESYYACFDTPLEPGARVVAPTRYGKDICTVLGTVKAPGFVRVDDIVRIDRVSTPE
                     DEARRVADEQKEKKAFELCQEKIKAHNLPMKLVFAHYLFDEQKVLFFFTADSRVDFRN
                     LVKDLVSIFKMRIELRQIGVRDEARVVGGCGVCGRVLCCHGVTDKLVPVSIKMAKDQN
                     LSLNSLKISGPCGRLLCCLAYEYGFYREARRGMPSEGVRIPYDGTVFRVVEVNVPAGR
                     LRLSGEDGRYVEAPSS"
BASE COUNT          265 a          483 c          484 g          240 t
ORIGIN      
        1 tcggcttcga cgcgccctcc gacgagcgcc gcggcgaggc cgtctccgcc ggggccgcat
       61 ggaaaaggta ggcctccccg acaacctgat ctcgagcctc gttcccgggc tgtcgggcca
      121 gcgcgccgat aagaagaaag aagccgccaa ggcgtcgccc aaggctctgc gcttcaggtc
      181 ggccctgaag gaggccgagg agatcgacct gacgccggcc gccgagggcc tgccgttctc
      241 cgaggccgag gccgccgagc tgctcgattc ggtccacgcc gccggcgagg agctcaagcg
      301 cgatcccgac cccgcctgcg tcaaggccta caagtcggcg gtacgcgatt tcgtccacta
      361 cgtcgtcggg cgcgcctacg atgtggtcga gaagagttcg ggcggcaata tcctgaaacg
      421 gaagaagttc accaccgtcg tcgtgataga cgagaagctc gagcgcctgg ccgcggaggt
      481 actctcggcc cagcgcgaca agctcgacat tctgcggcgc ctcgacgaga tccacggctt
      541 gctcgtggac ctcctgcgct gaatcggccg tcagggccgc taggagagac gatcatggat
      601 gacgatatgg actatatgca gggcgacgag gacctcagcc acctcgaaga cgacgccgta
      661 ccggccgcgg acgcgggcgt tcactgcggc gaactgcccg atcccctgta ccggctccgg
      721 gccgatccct acaacgagtc gtactacgcc tgcttcgata cccctctcga gccgggcgcc
      781 cgagtcgttg cgccgactcg ctacggcaag gacatctgca ccgtgctcgg cacggtcaag
      841 gcgcccggct tcgtgcgcgt cgacgacatc gtccggatag accgcgtctc gacgccagag
      901 gacgaggctc gcagggtggc cgatgagcag aaggagaaaa aagccttcga gctgtgccag
      961 gagaagatca aggcccataa cctgccgatg aagctcgtct tcgcccacta cctgttcgac
     1021 gagcagaagg tgctgttctt cttcaccgcc gactcgaggg tggacttccg caacctggtc
     1081 aaggatctgg tatccatttt caagatgcgt atcgagctac gccagatcgg cgtgcgcgac
     1141 gaggcgcggg tcgtcggcgg ctgcggggtc tgcgggcggg tgctctgctg tcacggcgtc
     1201 accgacaagc tcgtgcccgt gtcgatcaag atggccaagg accagaacct gtcgctcaac
     1261 tcgctcaaga tatccggccc ctgcgggcgc ctgctctgct gcctcgccta cgagtacggc
     1321 ttctaccgcg aggccaggcg cggcatgccg agcgagggcg tcaggatccc gtacgatggc
     1381 acggttttca gggtcgtgga ggtgaatgtg ccggcgggac ggctacgctt gtcgggagag
     1441 gacggccgct acgtcgaggc tccgtcctcc ca
//
LOCUS       DAOOLN010000589         1472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_113804_length_1472_cov_1.467890, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000589 DAOOLN010000000
VERSION     DAOOLN010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113804_length_1472_cov_1.467890"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PLW80_07880"
     CDS             <1..258
                     /locus_tag="PLW80_07880"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB66465.1"
                     /translation="VMFVTYARMKPRSAIIASMALHGLAYAFFFDAGFLYMDRVAPPE
                     IRGSAQALYTTVTQGLGLFAGTRITGAVMDRPKASGRKSSR"
     gene            complement(264..638)
                     /locus_tag="PLW80_07885"
     CDS             complement(264..638)
                     /locus_tag="PLW80_07885"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPB66466.1"
                     /translation="MNSTIVAPSLLAADFSAAGEALRLVESSGAPWLHLDVMDGRFVP
                     NLTFGPKMVADLRRRSKLFFDAHLMTVEPESLVDPFIEAGADAVTFHLEASVHAHRLV
                     QRIRDAIYGKLVGDQRRQVEAT"
     gene            783..>1472
                     /locus_tag="PLW80_07890"
     CDS             783..>1472
                     /locus_tag="PLW80_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66467.1"
                     /translation="MTRSTGVETLLTEAYDSLSALKLDAAQASFQSALEEDFEDEELL
                     YAMKCASWWSDSMDRVAGVSDAFEAGEYVMARWKAFRLFLAKLGPARDRPVSAFKQFA
                     FGVALQKFDSLADDGENADPEVLLMVGKANKGHGDYEAAVARLEAAAKLKRDDAAILA
                     ELADAYALIDEQRASKALFREAFYINPQRIDVELLESSAFARLAARVAELGKDGIEAA
                     EWIPVMGELLGV"
BASE COUNT          279 a          459 c          476 g          258 t
ORIGIN      
        1 gtgatgttcg tgacgtacgc ccggatgaag ccgcgttctg cgatcatagc ttccatggcc
       61 ctgcacggct tggcctacgc gttcttcttt gatgccgggt tcctatacat ggaccgtgtg
      121 gcacccccgg aaatccgcgg ttccgcgcaa gcgctctaca cgaccgtcac gcaggggctc
      181 ggcctcttcg ccggtacgcg gatcacgggt gcggtgatgg atcgaccgaa agcctctggc
      241 cggaaatcct cgagatgatg cggtcaggtc gcttcgacct gccttcgctg gtcacccacg
      301 agtttcccat agatcgcatc gcggatgcgc tggaccaggc ggtgggcgtg gaccgaggcc
      361 tcgagatgga aggtgaccgc gtcggccccg gcctcgatga acgggtcgac cagtgactcc
      421 ggctccacgg tcatcaggtg ggcgtcgaag aacagcttcg atcgcctgcg caggtcggcg
      481 accatcttgg gaccaaaagt caggttcggc acgaagcggc cgtccatcac gtcgaggtga
      541 agccagggag cgccggacga ctcgacgagg cgcagggcct ccccggcggc cgagaagtcg
      601 gccgctagca acgaaggagc gactatcgtg ctgttcatgg ccggattcta ccacgacgaa
      661 ggcctaggct caagctggag ctcgtcgtcc agccatggca tccccggccg acaagagatt
      721 cattgactta tcgggataat ttcatgtaca ataaaaaaca attcagcgga tacccgaggc
      781 ccatgacacg atccaccggc gtggaaacgc tactgacaga ggcatacgat tctctatcgg
      841 cgctcaaact ggacgcggcc caggcctcat tccagtcggc gctcgaggag gacttcgagg
      901 atgaggagct tctgtacgcg atgaagtgcg ccagctggtg gtccgactcg atggataggg
      961 tcgccggcgt ctccgacgcg ttcgaagccg gagagtacgt catggctcgc tggaaggctt
     1021 tcaggctctt cctagccaag ctcgggcccg ctcgcgatcg ccccgtctcg gccttcaagc
     1081 agttcgcctt cggcgtggcg ctccagaaat tcgatagcct ggccgacgac ggcgagaacg
     1141 ccgacccgga ggtgctactc atggtcggta aggcgaacaa gggccatggc gactacgagg
     1201 cggccgtggc gcggctcgag gccgcggcga agcttaagcg cgacgacgcg gccatcctcg
     1261 ccgagctagc cgacgcctac gccctgatcg atgagcagcg cgcctcgaag gccctgttca
     1321 gggaggcctt ctacatcaat ccccagcgca tagacgtcga gctgctcgag tcgagcgcct
     1381 tcgcacgtct cgccgccagg gtagccgagc tcggcaagga cggcatcgag gcggccgaat
     1441 ggatccctgt catgggcgag ctgctcgggg tg
//
LOCUS       DAOOLN010000590         1471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_113848_length_1471_cov_3.256356, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000590 DAOOLN010000000
VERSION     DAOOLN010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113848_length_1471_cov_3.256356"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1471
                     /locus_tag="PLW80_07895"
     CDS             <1..>1471
                     /locus_tag="PLW80_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66468.1"
                     /translation="SCYAQVAENFSFHVRPELDLPIGDKSALFFDDAAYRIGGNAALY
                     GQYLFPKAPFLYAEGGATFGLHPTQAADALTLASAGVGLGCDLRILDRVSLQLGAQGG
                     YSFGFFGAYDPAGNPYLGASLAANLDLSPGLALSAGGAYRYHVGWDPAAEAFTDLYQG
                     VGVWAGAILRLQPDSGRRKLRIADLRTEPLFPVFFGYYEHNPFGTMTVSNLENSAITN
                     VDVYFYVGEYMEQPMLTASIPSLGRRDSVEVPLKALFTSRLMNLTERAKVSVEVKVSY
                     TYLGERIAYSQPVTVTVLDRNAMTWDDDRKAASFVTPRDPTVMIFSKNTAGIIRDLGA
                     NPLNLNFRIAMGIFEAMRLYGLNYVIDPQSSFVEASKDELFLDYLQFPSQSLIYRAGD
                     CDDLSILYAALLESVGIETAFITVPGHIFMAFSLGLTELEARQAFTSAGDFIYADGKA
                     WVPVEVTIVQDGFIKAYKTGIKQWKEAVSKEVASFLPIH"
BASE COUNT          257 a          527 c          421 g          266 t
ORIGIN      
        1 tttcctgcta cgcccaggta gccgagaatt tctcgttcca tgtcaggccg gaactagacc
       61 tgcctatagg cgataagagc gcgctgttct tcgacgacgc ggcctatcga ataggcggca
      121 acgccgcgct ctatggccag tacctgttcc ccaaggcgcc cttcctgtac gccgagggag
      181 gcgcgacctt cggcctccat ccgacccagg ccgccgacgc cctgaccctg gcctcggccg
      241 gcgtcggcct cggctgcgac ctgaggatac tcgaccgcgt cagcttgcag ctcggagccc
      301 agggcggcta ctcgttcggc ttcttcggcg cctacgaccc cgcgggcaac ccctacctcg
      361 gcgctagcct cgccgccaac ttggacctga gccccggcct ggccttatcg gcaggcggcg
      421 cttaccgcta ccacgtcggc tgggatcccg ccgccgaagc ctttaccgac ctgtaccagg
      481 gcgtcggcgt ctgggccggc gcgatactca ggctccagcc cgactccggc cgtcgcaagc
      541 tcaggatagc cgacctgcgg accgagccgc tcttccccgt attcttcggc tactacgagc
      601 ataacccctt cggcacgatg accgtctcca acctcgagaa cagcgcgatc accaacgtcg
      661 acgtgtactt ctacgtcggc gagtacatgg aacagccgat gctgacggcg agcatcccgt
      721 cgctcggccg gcgcgacagc gttgaggtgc cgctcaaggc cctcttcacg agccgcctca
      781 tgaacctgac cgagcgggcc aaggtctcgg tcgaggtcaa agtctcgtac acctacctcg
      841 gcgagcgtat cgcctactcg cagccggtta ccgtcaccgt gctcgaccgc aatgccatga
      901 cctgggacga cgaccgcaag gcggcatcgt tcgtaacccc acgcgacccg accgtaatga
      961 tcttctcgaa gaacaccgcg ggcatcatcc gcgacctcgg cgcgaatccc ctgaacctga
     1021 atttcaggat agcgatgggc atcttcgagg cgatgcggct ctacgggctc aactacgtga
     1081 tcgaccccca gtcgtccttc gtcgaggcgt ccaaggacga gctgtttctc gactacctgc
     1141 agttcccgtc ccagtcgctc atctaccgcg ccggcgactg cgacgacctg tccatcctgt
     1201 acgcggccct gctcgagtcg gtcggtatcg agacggcctt catcaccgtg ccaggccata
     1261 tcttcatggc cttctcgctc gggctgaccg agctcgaggc caggcaggcc ttcacgagcg
     1321 cgggcgactt catctacgcg gacggcaagg cctgggtacc cgtcgaggtg accatcgtgc
     1381 aggacggctt catcaaggcc tacaagacgg gtatcaagca gtggaaggag gccgtctcca
     1441 aggaggtcgc ctcgttcctc ccgatccatg a
//
LOCUS       DAOOLN010000591         1470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_113969_length_1470_cov_3.707420, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000591 DAOOLN010000000
VERSION     DAOOLN010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113969_length_1470_cov_3.707420"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1470)
                     /locus_tag="PLW80_07900"
     CDS             complement(<1..>1470)
                     /locus_tag="PLW80_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66469.1"
                     /translation="DPRERQNLDALAKRMRGARLAPRLVGETLPEAVLIGDEGSRPLS
                     RSMTGITERAIAASVEDNPEALSTALFPIIGAAIRKALNKMLAEMMESMNSGLERAFS
                     FKRLGWRFESWKTGTPFVEIVLRETLRYRVEHVFLIHSKTGLLLREASPPGVVSPDSD
                     MVASMLEAVREYIKDSLALAKTDAVQSIRTGSYALLVEDGPYASIALFVRGSPDPSLR
                     NAAQEAVETIHLRLGSRLKSFSGDVGPFEEADPALRRCLLSQDTRAPGGKPVYAIVAL
                     SAILLAAMTAVGFGIAAARSREAFERALDEEPGIVLVDSRRSRGRRVLRVLRDPGAAT
                     VAELAEGMGVDAAAFVVEEEPYWSPELRPGVPVAEDAPLVVPEELLELARRLGGIVIL
                     FEQDSGELRTGQQAAIRAAGDLLSELIGKSRAYGLDVSVDIVGHAAGEVQDEAGLRVS
                     EERAQKALAMFAELNAPLVDYVRPVGVGVAEPVAPTDGA"
BASE COUNT          235 a          519 c          494 g          222 t
ORIGIN      
        1 cggcaccatc agtcggcgcc acgggctccg cgacgccgac gccgaccggc cgcacgtagt
       61 ctacgagcgg ggcgttcagc tcggcgaaca tggccagggc cttctgcgcc cgctcctcgc
      121 tgacgcggag accggcctcg tcctgaacct cgccggcggc gtgcccgact atatccaccg
      181 acacgtccag gccgtatgcg cgcgacttgc ctataagctc ggacaagagg tcgccggccg
      241 cgcggatggc cgcctgctgc cccgtccgta gctcacccga gtcctgctcg aacaggatga
      301 cgatgccgcc aagccgcctg gccagctcga gaagctcctc cggcacgacg agcggggcgt
      361 cttcggctac cgggacgccg ggcctgagtt cgggcgacca gtacggctcc tcctcgacga
      421 cgaacgcagc agcgtcgacg cccatgccct cggccagttc ggctacggtc gcggccccgg
      481 ggtcgcggag cacccgtagc acgcgccggc cgcgcgatcg ccgcgagtcg accaggacga
      541 tgccgggctc ctcgtcgagc gcgcgctcga aggcctcccg ggacctcgcc gccgctatgc
      601 cgaagccgac cgcggtcatg gccgccagga ggatcgcgct cagggcgacg atggcgtaga
      661 cgggcttgcc acccggcgcc cgggtatcct ggctaagcag gcagcggcgc agggccggat
      721 cggcttcctc gaaggggccg acgtcgcccg agaagctctt gagccgcgag ccgagccgca
      781 ggtggatcgt ctcgacggcc tcctgggcgg cgttccggag cgacgggtcg ggcgagccgc
      841 ggacgaacaa ggctatggac gcgtagggac catcctcgac taacagagca taggagccag
      901 tcctgatgga ctggacggcg tccgtcttgg ccagcgcgag cgagtccttg atatactccc
      961 tgaccgcctc gagcatcgag gcgaccatgt cgctgtcggg cgatacgacg ccgggaggcg
     1021 acgcctcgcg aagaaggagt ccggtcttcg aatggatcag gaacacgtgc tcgacgcggt
     1081 agcgtagcgt ctccctgagc acgatttcga cgaagggcgt tccggtcttc cacgactcga
     1141 atcgccagcc caggcgcttg aacgagaagg ctcgctcgag cccgctgttc atcgactcca
     1201 tcatctcggc cagcatcttg ttcagggcct tgcgtatggc ggcgccgatt atcgggaaca
     1261 aggcagtcga gagcgcctcg ggattatcct cgacggacgc ggctatggcc cgttcggtta
     1321 ttcccgtcat cgacctggac aggggccgcg agccctcgtc gcctatgagc acggcttcag
     1381 gcagcgtctc gcctaccagg cgcggcgcca gcctggcgcc acgcatccgc tttgccagcg
     1441 cgtccaggtt ctggcgctcc cgcgggtcca
//
LOCUS       DAOOLN010000592         1470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_114030_length_1470_cov_2.149823, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000592 DAOOLN010000000
VERSION     DAOOLN010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114030_length_1470_cov_2.149823"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PLW80_07905"
     CDS             complement(<1..663)
                     /locus_tag="PLW80_07905"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HPB66470.1"
                     /translation="MHDRSSIAPLSDSEFRKIASFIEQEFGIRMPEAKKALLQGRLHK
                     RLVACGKRSYGEYFGFVTEDPAGKDEFLVFTDLVSTHETSFFREPGHFKHLRQILPDL
                     AAERSRLRFLCAACSTGEEAYSLAMTIDETLSEAGLGRVPFTIEGFDLSSHAVEAARR
                     GVFSEARAKKIPAEMTKRYMMRSKNPGKEVRRAVPELRARMLFHTGNLLDDMGLAERR
                     YDV"
     gene            complement(665..1225)
                     /locus_tag="PLW80_07910"
     CDS             complement(665..1225)
                     /locus_tag="PLW80_07910"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPB66471.1"
                     /translation="MTSTIDTNPAAAPDTARGGATGQYLLFELAGEHYAFDVVRAKEV
                     LEDVSITPLPTKAPFLSGVINLRGSIIPIIDLRTKFEISGRGGRGSVIVIEIGDKDRQ
                     AIVGALVDSVKGVTRFGPDELEAPPKFGMRVESALVKAIAKQSGLFVVILDSELLFSE
                     RHLWSDGPAGIQAGTPESTTSGIRVG"
     gene            complement(1228..>1470)
                     /locus_tag="PLW80_07915"
     CDS             complement(1228..>1470)
                     /locus_tag="PLW80_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66472.1"
                     /translation="AFLKTGKRNLGAYDSPRPRTAAHTAGSRLGSGVAVKRPATDAGE
                     GPSHVAPKAQDKRPHAGNTAITIAHADTEDDEFERF"
BASE COUNT          230 a          473 c          475 g          292 t
ORIGIN      
        1 cacgtcgtac cgacgttccg ccaggcccat gtcgtcgagc aggttgccag tatggaacag
       61 catgcgcgcg cgcagctcgg gcacggcacg gcggacctcc ttgcccgggt tcttgctgcg
      121 catcatgtag cgcttcgtca tctcggccgg tatcttcttg gcgcgcgcct cggagaagac
      181 gccgcgcctc gcggcttcga ccgcgtgcga cgacaggtcg aagccctcga tcgtgaacgg
      241 tacgcgaccg agccccgcct ccgacagcgt ctcgtcgatg gtcatcgcga ggctataggc
      301 ttcctctccc gtggagcagg ccgcgcatag gaagcgtaga cgcgagcgct cggcggctaa
      361 gtccggcagg atctgcctga ggtgcttgaa atggccgggc tccctgaaga agctggtctc
      421 gtgggttgaa accaagtcgg taaagaccag aaattcgtcc ttgccggccg ggtcctcggt
      481 cacgaagccg aagtactcgc cgtagctacg cttgccgcag gccacgagcc gcttgtgcag
      541 gcggccctgc aggagcgcct tcttggcctc cggcatgcgt ataccgaact cctgttcgat
      601 gaacgacgct atcttcctga actcgctatc ggacagcggc gctatgctcg atcggtcgtg
      661 catctcagcc tactcggatc ccggatgtcg tcgactcggg tgtcccggcc tggattccag
      721 cgggaccgtc gctccagagg tggcgctccg agaatagcag ctcggaatcc aggatgacga
      781 cgaagagccc cgactgcttg gctatcgcct tgacgagcgc ggactcgacg cgcatgccga
      841 acttgggcgg cgcctcgagc tcatccgggc cgaagcgagt gacgcctttg accgagtcga
      901 caagggcgcc gactatggcc tgcctgtcct tgtcccctat ttcaatgacg atgaccgagc
      961 ctcggccgcc gcgtccgctt atttcgaatt tcgtccgcag gtcgatgatg gggatgatgc
     1021 taccgcggag gttgatcacg cccgacagga agggcgcctt ggtcggtagc ggcgtgatcg
     1081 agacgtcctc gagcacctcc ttggccctga ccacatcgaa ggcgtagtgc tcgccggcca
     1141 gttcgaataa aaggtactgc cccgtcgccc ctcccctggc ggtgtccggt gccgcggcgg
     1201 ggttcgtgtc tatcgtcgat gtcatgctca gaacctttcg aattcgtcgt cctctgtatc
     1261 ggcgtgggcg atcgtgatgg ccgtattgcc tgcgtgcggc ctcttatcct gcgccttagg
     1321 cgcgacgtgg gatggacctt cgccggcgtc cgtcgccggc cgcttgacgg cgacgcctga
     1381 gccgagccgg ctcccggcgg tatgggccgc ggtccgaggg cgcgggctat cgtaggcgcc
     1441 caggttgcgt ttgccagttt ttaggaacgc
//
LOCUS       DAOOLN010000593         1467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_114334_length_1467_cov_3.155099, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000593 DAOOLN010000000
VERSION     DAOOLN010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114334_length_1467_cov_3.155099"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PLW80_07920"
     CDS             <1..468
                     /locus_tag="PLW80_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66473.1"
                     /translation="LWDLYANIDPRLAWRTDKTRFGVVWDPIREFMYREADDGVPPFA
                     PGQVLVLELRLAGFYRLPVAFRIREIDRASGVIEFVYLEANKSNGKQRLEFAPAIGPD
                     GERRSTIRHSTWYRSGDEFRDERLYGGFHASIIDAFHRAVVESAGYSIAVVES"
     gene            465..>1467
                     /locus_tag="PLW80_07925"
     CDS             465..>1467
                     /locus_tag="PLW80_07925"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPB66474.1"
                     /translation="MNRRIEATPGETTEPTRLARRLSPVDVMTVAFIVGNIVFIALAR
                     NSIAEPLPLIGGYLGCLAVSCAAVAIGGPERYAPPRGGLARALRWFQGLVRQGYPLPL
                     FLFFFVAVTRFDTALFRDNLDPFFASLDERLFGFVPSWTLMIEHPSLFLSELLSGAYV
                     LYYASIGVLAIWLYVKDRPAMAEYVFVVMLLVYAACLTYIVLPVVGGRYDPTIRELTE
                     TYRYGPFTRLMSFIYRRSAHEGAAFPSTHATVSLVVALFSLRRARPLAPLFCVNAALI
                     LVATIYCGYHYVVDVLAALVYVAALYPAGLRLYAALGDRESRLVDTIDDSRGGIDEAG
                     "
BASE COUNT          214 a          492 c          465 g          296 t
ORIGIN      
        1 ctatgggacc tgtacgcaaa catcgatccg cgtttggcgt ggcgtaccga taagacccgt
       61 ttcggggtgg tatgggaccc gatacgcgag ttcatgtacc gcgaggctga cgacggcgtg
      121 ccgccgttcg ccccgggcca ggtgctggtg ctcgagctca ggctggccgg attctatcgc
      181 ctgccggtcg ccttcaggat acgggagatc gaccgcgcct cgggcgtgat cgaattcgtc
      241 tacctcgagg cgaacaagtc gaacggcaag cagcgcctcg aattcgcgcc ggcgataggc
      301 cctgacggcg agcggcgctc gaccatacgt catagcacct ggtaccgttc cggcgacgag
      361 ttcagggacg agcgcctgta cggcgggttc cacgcgtcga tcatcgacgc gttccaccgg
      421 gccgtcgtcg aatcggccgg ctactccatc gccgtggtcg aatcatgaac cgacgcatag
      481 aggctactcc gggagagacg acggagccga cgcggctagc gcgtcggctg tctccggtcg
      541 acgtcatgac cgtcgccttc atcgtcggca acatcgtatt catcgcgctc gcccggaatt
      601 cgatagccga gccgctgccg ctcatcggcg gctacctcgg ctgtctggcc gtctcatgcg
      661 ccgccgtcgc gatcggcggg ccggagcgct acgcgccgcc ccgcggtggg ctggcccggg
      721 cgctgcgatg gttccagggc ttggtccgcc agggttatcc gttgccgctg ttcctgttct
      781 tcttcgtcgc ggtgactcgt ttcgataccg cgctgttcag ggataacctc gacccgttct
      841 tcgcctcgct cgacgaacgg ctgttcggtt tcgtgccgag ctggaccctg atgatcgagc
      901 atccgtcgct cttcctttcg gagctcctga gcggggccta cgtgctgtac tacgcgtcca
      961 tcggcgtcct ggcgatctgg ctatacgtca aggatcgccc cgcgatggcc gaatatgtct
     1021 tcgtcgtcat gctactcgtc tacgcggcct gcctgaccta catcgtcctg cccgtcgtcg
     1081 gggggcggta cgacccgacc atacgagagc tgaccgagac ctaccgctac ggccccttca
     1141 cgaggctgat gagcttcatt tatcgccgta gcgcccacga gggcgccgcc ttcccgagca
     1201 cgcatgccac cgtcagcctg gtcgtcgcgc tgttctcgct caggcgggcc cgccccttgg
     1261 cgccgctatt ctgcgtcaac gccgcgctga tccttgtggc gaccatctac tgcggctatc
     1321 actacgtggt ggacgtgctc gccgccctgg tctacgtcgc cgcgttgtat ccggcgggcc
     1381 tcaggctgta tgcggcgcta ggggatcgcg agtctcggct agtggacaca atcgacgact
     1441 cacgaggcgg gatcgacgag gcaggct
//
LOCUS       DAOOLN010000594         1460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_115190_length_1460_cov_3.121708, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000594 DAOOLN010000000
VERSION     DAOOLN010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115190_length_1460_cov_3.121708"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /locus_tag="PLW80_07930"
     CDS             <1..1267
                     /locus_tag="PLW80_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPB66475.1"
                     /translation="GREGFLNRALMAAFGLDEPPLRFLSSFAGVVMAHGFYNFPVVMR
                     FVGDAWATAPRRHEDAARLLGASERRVFMTVTLPSILPSIGAAMSLVFLMCFYSFVIV
                     LLFGGAGVGTPEVELYRAARFEFDRPLASAFALVETVVAMGALALYAAFERRAAGDRE
                     ESERVAPNGFRSSGGIALAVLYGAFIAVCFVGPLASIVAESLAIRNASRVGSSLGLGN
                     YVSLFTRRGFVPALANTALLGLLCAALASVAGFTLSIGLKRARSPFLSKTLPLLPLGV
                     SGVVLAYGWNSALGRSSTLAVAAVQAVSAYPFALRAIQSSVGLSDERYVEAARCLGSS
                     RLGAALRVRLPMAASSIASGFAFAFAMSAGDANALIAAPVAGFETLALMLYRLAGSYR
                     LNEACAAAVLLAIGTGFIFFIKDASDVRL"
     gene            1254..>1460
                     /locus_tag="PLW80_07935"
     CDS             1254..>1460
                     /locus_tag="PLW80_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB66476.1"
                     /translation="MSAFDSVRLELVELTKAWPGRTVSVSMTAEAGGILAVAGPSGCG
                     KSTALRMAAGLECPDSGRVIVGGRD"
BASE COUNT          167 a          516 c          497 g          280 t
ORIGIN      
        1 cggccgtgaa ggattcctga accgcgcgct gatggcggcg ttcgggctcg acgagccgcc
       61 tctacgcttc ctctcctcgt tcgccggcgt cgtgatggcc cacggcttct ataatttccc
      121 cgtcgtgatg cgcttcgtcg gcgacgcctg ggcgacagcg ccgcgccgcc acgaggacgc
      181 ggccaggctg ctcggcgcct ccgagcgccg agtgttcatg acggtgaccc tgccgtccat
      241 cctaccgtcc atcggcgccg cgatgtccct cgtcttcctc atgtgcttct atagcttcgt
      301 catcgtgctg ctgttcgggg gggcgggcgt cgggacgccc gaggtcgagc tgtaccgggc
      361 cgcccgcttc gagttcgacc gtccgctggc gagcgccttc gcgctcgtcg agacggtcgt
      421 cgcgatgggc gcgctggcgc tgtacgccgc cttcgagcgc cgggccgcgg gtgaccgcga
      481 ggagtcggag cgcgtagcgc cgaacggctt ccgaagctcg ggcggaatcg cgctcgccgt
      541 cctgtacggg gccttcatag ccgtctgttt cgtcggccca ctggcctcga tagtcgcgga
      601 atcgctcgcg atcaggaacg cctccagggt cggctcgagc ctcggattgg gcaactacgt
      661 ctcgctgttc acgaggaggg gcttcgtccc ggccctggcc aacaccgcgc tcctggggct
      721 cctctgcgcc gcgctcgcga gcgtggctgg cttcacgcta tccataggcc tcaagcgagc
      781 ccgctcgccc ttcttgtcca agaccctacc cctcttgccg ctcggcgtat cgggcgtcgt
      841 gctcgcctac ggttggaact cggcgctcgg gcggagttcc accttggccg tcgcggcggt
      901 gcaggccgtc tcggcctacc ccttcgcgct acgcgccatc cagtcgtcgg tgggcctttc
      961 cgacgagcgc tacgtcgagg cggcccgctg cctcggctcg tcgcggctcg gcgccgcgct
     1021 gcgcgtacgg ctaccgatgg ccgcctcctc gatagcctct ggtttcgcgt tcgcgttcgc
     1081 gatgagcgcc ggcgacgcga acgcgttgat agcggccccg gtcgccggct tcgagacgct
     1141 ggccctgatg ctctaccgat tggccggttc ctataggctg aacgaggcct gcgccgcggc
     1201 ggtgctgctg gccatcggaa ctggcttcat cttcttcatc aaggacgcga gcgatgtccg
     1261 cctttgattc agtacgcctg gagctggtcg agctgaccaa ggcttggccc ggccggacgg
     1321 tcagcgtatc catgaccgct gaggccggcg gcatcctggc cgtggccggg ccctccggtt
     1381 gcggcaagag cacggcgctc aggatggccg cggggctcga gtgcccggac tcgggccggg
     1441 ttatcgtcgg agggcgcgac
//
LOCUS       DAOOLN010000595         1459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_115304_length_1459_cov_3.580484, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000595 DAOOLN010000000
VERSION     DAOOLN010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115304_length_1459_cov_3.580484"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PLW80_07940"
     CDS             <1..812
                     /locus_tag="PLW80_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66477.1"
                     /translation="LRLLPPIRRMRGNRLYAADGRRFLDLWLDDGRGILGHRDRLART
                     AAANAADKGLLRPYPGLYDARLAKSVSSAWPGFGTVRAFESDERAVQAAGRVAARIEA
                     QSSGSSSIQVGGQRREPYLARPFAPVPDGLDLVMPRLPCPRPFAPACLAARDGSPAAQ
                     ALAAEPGDLVSAQACLAAARGLASLGSAAADGYDETLWKRFDRRMAAFFEREGPYLRA
                     RVPSERYEAFFRAALTGGALVSPEPGSPSVVPPEFDDGELVKLARSLESSL"
     gene            complement(809..>1459)
                     /locus_tag="PLW80_07945"
     CDS             complement(809..>1459)
                     /locus_tag="PLW80_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPB66478.1"
                     /translation="LLGAFGFAPQAGLFFVTVRFLDPGIASLLLYLYPAFVVGFWFLF
                     FGRRPRREQLIALALSGAGCVLTLWTRGSYPLIGYLLGLSVAVSYAGYLVIGERVTVG
                     IDPTFLTANLMTVSAVVYWIVTLASGSFLLPSTPASIAGVVGIAVVGSVIPIVTLFAS
                     IRIIGSSDASLVSTIEPLFTVSLSALLLGERLTPLQLAGGALILLGVLLLNLRKRR"
BASE COUNT          245 a          507 c          510 g          197 t
ORIGIN      
        1 tcttgagatt attgccgccg atacgccgga tgcgcggcaa ccgactctac gccgccgacg
       61 gcaggcgctt cctcgacctg tggctcgacg acggccgcgg tatcctcggc catcgcgacc
      121 gcctcgcgcg tacggccgcc gccaacgccg ccgacaaggg cctcctacgg ccctatcctg
      181 ggctatacga cgcgcgcctc gccaagtccg tgtcgtcggc ctggccgggc ttcggtacgg
      241 tgcgggcctt cgagagcgac gagagagccg tccaggcggc gggccgcgtg gccgcgcgca
      301 tcgaagctca atcgagcgga tcgtcctcca tccaggtcgg aggacagcgg cgcgagccgt
      361 atctcgccag gcccttcgcg ccggtccctg acggtcttga cctcgtcatg ccgcgcctac
      421 cctgcccgag gcccttcgcg ccggcctgcc tcgcggcccg cgacggtagc ccggcggcgc
      481 aggcgctcgc ggccgagccg ggcgacctcg tgtcagccca ggcctgcctg gcggccgcgc
      541 gcggtctcgc cagcctcggg tcggcggcgg ccgacggcta cgacgagacg ctgtggaaac
      601 gcttcgaccg ccgcatggcc gccttctttg agcgcgaggg tccctacctg cgagccaggg
      661 tgccgtccga acgctacgag gctttcttca gggccgcgct cacgggcggc gccctcgtat
      721 cgcccgagcc gggctcgccc tcggtcgtgc cgcccgagtt cgacgacggc gaactcgtca
      781 agctggccag gagcctcgag tcgtcgctct agcgccgctt acgcaggttc aggagcagca
      841 cgccgaggag gatcagcgcc ccgccggcta gctggagggg cgtcagccgc tcgccgagca
      901 gcagcgccga taggctcacg gtgaacagcg gctctatcgt cgacacgagc gacgcgtcgg
      961 acgagccgat gatgcggatg gacgcgaaca gcgtgacgat gggtatgacc gatccgacca
     1021 cggcgatacc gacgacgccg gctatcgacg cgggcgttga aggcagcagg aacgagccgg
     1081 aggctagcgt gacgatccag tagacgacgg ccgataccgt catcaggttg gccgtcagga
     1141 aggtcgggtc gatgccgacg gtgacgcgct ccccgatgac gaggtagccg gcgtagctga
     1201 cggctaccga caagccgagt aggtagccga tgagcggata cgagcccctg gtccatagag
     1261 tcaggacgca accggcgccg gatagcgcca gggcgatcag ctgctcgcgc ctaggcctgc
     1321 ggccgaaaaa caggaaccag aacccgacta cgaaggccgg gtataggtat aggagcagcg
     1381 acgctatccc cgggtcgagg aacctgaccg tgacgaagaa cagcccggcc tggggcgcga
     1441 agccgaaggc gccgagcag
//
LOCUS       DAOOLN010000596         1458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_115429_length_1458_cov_3.155381, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000596 DAOOLN010000000
VERSION     DAOOLN010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115429_length_1458_cov_3.155381"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1458)
                     /locus_tag="PLW80_07950"
     CDS             complement(<1..>1458)
                     /locus_tag="PLW80_07950"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HPB66479.1"
                     /translation="AVLSGRDALAVLPTGGGKSLCYQVPALILGGTTLVVSPLIALMR
                     DQVAALRAAGVEAVFINSSLEAAERREIEARVRSGGVRLVYAAPESLAGDRLLSLLDD
                     APPRLIVVDEAHCVSEWGHDFRPEYRGLGYLRERYPAAVWMATTATATERVRADIARC
                     LGLRDPALFLGGFDRPNLSIRVERKLEVRHRIVRFAQSRPDAAGIVYCGSRRRTEELA
                     DALRVAGVKAAAYHAGLPSEERSWVQEAFVRDDLLVVVATVAFGMGIDKPDVRYVVHA
                     DLPKSVENYYQEIGRAGRDGLPSECVLFYGAGDVAAASRLFDELPDEQRRAALSRLEA
                     MRDYAESERCRRALILEHFGEEGGADCGRCDNCLAGPRASVDLSIPAQKLLSCVKRTG
                     ERFGAAHIVDVLLGERTEKVERFSHDGLTTFGIGTELGRSAWMALARRLQSTGHLVRE
                     PERQTLSLGRPAYELFRDRGPYLVPVDLIGGSTNG"
BASE COUNT          222 a          527 c          507 g          202 t
ORIGIN      
        1 cgccgttcgt cgagccgccg atgaggtcga ccggtacgag gtagggtccc cggtctctaa
       61 acagttcgta ggccggacgc ccgagcgaca gagtctggcg ctccggctcc cgcacgaggt
      121 ggccggtcga ctggagccgc ctcgccagcg ccatccacgc gctccggcca agttccgtgc
      181 cgatgccgaa ggtggtcagg ccgtcgtggc tgaagcgctc gaccttctcg gtccgctccc
      241 ccaggagcac gtcgacgata tgggccgcgc cgaagcgctc gccggtccgc ttgacgcacg
      301 acagcagctt ctgggccggg atcgacaggt cgacgctagc gcgtgggccg gcgaggcagt
      361 tgtcgcaacg gccgcagtcg gcgccgccct cctcgccgaa atgctcgagg atcagcgccc
      421 gccggcagcg ctcgctctcg gcgtagtcgc gcatcgcctc gagcctcgag agcgcagccc
      481 ggcgctgctc gtcggggagc tcgtcgaata gcctggaggc cgccgccacg tcgccggctc
      541 cgtagaacag cacgcactcg gacggcaggc catcgcgtcc ggcccgtcct atttcctgat
      601 agtagttctc gacgctcttc ggcaggtcgg cgtggacgac gtagcgtacg tcgggcttat
      661 cgatgcccat gccgaaggcg acggtggcca cgacgacgag gaggtcgtcc cgcacgaagg
      721 cctcctgtac ccaggagcgc tcttcagatg gaagccccgc atggtaggcg gcggccttga
      781 cgccggcaac ccgtagcgca tccgccagct cctcggtcct gcgcctggag ccgcaataga
      841 cgatgcccgc ggcgtcgggc ctggactgcg cgaagcgcac gatcctgtgc cgtacttcta
      901 gcttccgctc gacccggatg gacaggttcg gccggtcgaa gccgccgagg aacagcgccg
      961 ggtcgcgcag cccgaggcaa cgcgcaatgt cggcgcggac gcgctcggtc gccgtggccg
     1021 tggtcgccat ccagacggcg gccgggtagc gctcgcgtag gtagccgagg ccacggtact
     1081 ccggccggaa atcgtggccc cactcggaga cgcagtgggc ctcgtcgacg acgatcagcc
     1141 tgggcggcgc gtcgtcgagc agcgacagga gccgatcgcc ggccagcgac tccggcgcgg
     1201 cgtacacgag gcgtacgccc cccgagcgca ccctggcctc gatctcgcgc cgctcagcgg
     1261 cctcgagcga gctgttaatg aagaccgcct cgacgccggc ggcgcgcagc gcggccacct
     1321 ggtcgcgcat cagcgcgatc agcggcgaga cgaccagcgt cgtcccgccc aggatcagcg
     1381 ccggtacctg gtagcacagc gacttgccgc cgccggtcgg cagaacggcc agcgcgtctc
     1441 gcccggacag gaccgcgg
//
LOCUS       DAOOLN010000597         1457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_115559_length_1457_cov_3.086305, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000597 DAOOLN010000000
VERSION     DAOOLN010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115559_length_1457_cov_3.086305"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PLW80_07955"
     CDS             complement(<1..753)
                     /locus_tag="PLW80_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66480.1"
                     /translation="MKGILDKTRALETSLAEMKELLLGNVVLLGEVPSYAGTWQSDDE
                     MSSVDYSVRARFFADRIGELGVDECTSDPLGNPIGIIKGGEPGRPPILVCAELDSLFN
                     PPGDIHYAIDGDTIIGPGLMDNAIGAAAVMSLPDVLKREGLAFKSTIMLAGIANTMRD
                     SKNLTLVERFLESLGTKPGAAIIVKGGELGRLNYFSEAVVRADILCERRPGARQSPPN
                     MIIVANEIIDRLLELELPQKPETAVTVGMIRGG"
     gene            981..>1457
                     /locus_tag="PLW80_07960"
     CDS             981..>1457
                     /locus_tag="PLW80_07960"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPB66481.1"
                     /translation="MTNIESLIPDDFSGCVSVSRDGSIVFRKAFGYADKANERLNTVD
                     TRFATASAGKFFVAIAVMRLVEEGRLGLEDPIGSILRFDLRAIDPGVTIRQLLTHTSG
                     VPDYFDESVMDEYADLWVDFPCYRIRRSADLLPLFVDKPMMYPAGQRFQYNNSGFVL"
BASE COUNT          254 a          475 c          455 g          273 t
ORIGIN      
        1 gcccccgcgt atcatgccca ccgtgacggc cgtctccggc ttctgcggca gctccagctc
       61 gaggagccgg tcgatgatct cgttggcgac gatgatcatg ttcggcggcg actggcgcgc
      121 tcccggccgg cgctcgcaga ggatatcggc gcggaccacg gcttccgaga aatagttgag
      181 ccgacccagc tcgccgccct tgacgatgat ggccgcgccc ggcttcgtgc cgaggctctc
      241 caggaagcgt tcgacgagcg tcaggttctt gctatcgcgc atcgtattgg cgatgccggc
      301 cagcatgatc gtcgacttga acgcgaggcc ctcccgcttg agcacgtccg gaagcgacat
      361 gaccgcagcg gcgccgatag cgttgtccat gagcccgggc ccgatgatgg tatcgccgtc
      421 gatggcgtag tggatgtcgc cgggagggtt gaacagggag tcgagctcgg cgcagacgag
      481 tatgggcgga cgcccgggct cgccgccctt gatgatgcca atgggattgc cgagcggatc
      541 gctggtgcac tcgtcgacgc cgagctcgcc gatgcgatcg gcgaagaacc gcgcgcgcac
      601 gctatagtcg acgctcgaca tctcgtcgtc gctctgccag gtgcccgcgt acgacggcac
      661 ctccccgagc agcaccacgt tcccgagcaa cagctccttc atctccgcga gcgacgtttc
      721 gagcgcccgc gtcttgtcca agataccctt caagacggct ggatccagtc tgtacctcct
      781 cgcgaccgtg gcctacggct gattcccggg ccggccacga tccagtatac gggatagcgc
      841 ccgagctggc aaacgtccgt ctgcgatgga gccccgagcc gtctactttc ctggtttaca
      901 cgatgagtcg cttgcctcgg ccctccgatc gagctttaat gatcgcatgc gatcaagctc
      961 aaggaacaag gaaagcgtgt atgacgaaca tcgaaagcct cataccggac gacttcagcg
     1021 gttgcgtctc cgtatcgcga gacggttcca tcgtgttcag gaaggccttc ggctacgccg
     1081 acaaggccaa cgagcggctg aacacggtcg acacgaggtt cgccaccgcg tcggccggca
     1141 agttcttcgt cgccatcgcc gtcatgcggc tcgtcgagga agggcggctg gggctcgaag
     1201 atcccatcgg ctcgatactg cgcttcgact tgcgcgctat cgaccctggc gtgacgatca
     1261 ggcagctcct gacgcatacc tcgggcgtgc cggactactt cgacgagagc gtgatggacg
     1321 agtacgcgga tctatgggtc gatttcccct gctaccgtat caggcgctcg gccgacctgc
     1381 taccgctgtt cgtcgacaag ccgatgatgt atccggccgg ccagcggttc cagtacaaca
     1441 actcgggctt cgtgctg
//
LOCUS       DAOOLN010000598         1456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_115696_length_1456_cov_2.862241, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000598 DAOOLN010000000
VERSION     DAOOLN010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115696_length_1456_cov_2.862241"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..771)
                     /locus_tag="PLW80_07965"
     CDS             complement(73..771)
                     /locus_tag="PLW80_07965"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HPB66482.1"
                     /translation="MKAIDKATIWIGALTAALLAATAVLYARLPVSIPIHWDSAGAAD
                     GWAAKPWAFLLASSPVLATALVRIAAAVDPRYSNIKAREGTFVVVVVSVAALAASLHL
                     ITIVTALGLLEGTDLRLVPLATGLAFVAIGNVMPRLKWNYTVGIRLPWTLADEGVWRA
                     THRFGGVAFVLAGLATAATAFMPERAARFVGGGAILSCVAATTAYSYAVHRSRRDLGR
                     NYIDSEGGNDEHRS"
     gene            complement(768..1031)
                     /locus_tag="PLW80_07970"
     CDS             complement(768..1031)
                     /locus_tag="PLW80_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HPB66483.1"
                     /translation="MNKIWKALDDPTRRRILELLKAGPRSAGELGAEFDMSAASMSHH
                     LGMLADAGLVEREKKGQFVYYELNSTVFQSLLEWIMAMKGQDR"
     gene            1174..>1456
                     /locus_tag="PLW80_07975"
     CDS             1174..>1456
                     /locus_tag="PLW80_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66484.1"
                     /translation="MIHTRRTTALAVIQFTLTMLPLAAWSQALPRDASKAGFIATAGK
                     RMASFEVKSLTGSSLSSKDLAGSPALFHIWSARDDPAGNGPALAASLREA"
BASE COUNT          267 a          476 c          447 g          266 t
ORIGIN      
        1 tatttgccga aggcgtccca catcgccatt tcgacggcgc acagcgccat gacgaggcca
       61 cgtagcgtcg atttacgatc tatgttcatc attccctcct tcagaatcga tgtagttccg
      121 gccgaggtca cgtcgcgatc ggtgtacggc gtacgagtag gccgtggtcg ccgcgacaca
      181 ggataggatc gcgcctccac cgacaaaccg agccgcccgc tctggcatga aggccgtcgc
      241 ggccgtcgcc aggccggcca gcacgaaggc gacgccgccg aagcggtgcg tagccctcca
      301 gacgccttcg tcggccagcg tccagggcag gcggatgccg accgtgtagt tccacttgag
      361 gcgcggcatc acgttgccga tggcgacgaa ggccaggccg gtcgcgagcg gcacgagcct
      421 caggtcggtg ccttcgagca gtccgagggc ggtcacgatc gttatcaggt gcagggaggc
      481 tgccagcgcg gccaccgata cgacgacgac gacgaaggtc ccctcgcgcg ccttgatgtt
      541 gctgtagcga gggtcgacgg ccgccgcgat ccggaccagt gcggtcgcca gtacgggcga
      601 cgaggctagc aggaaggccc agggtttggc ggcccagccg tcggccgcgc cggccgagtc
      661 ccagtgtatc ggtatcgata cgggcaggcg cgcgtacagg accgcggtcg ccgccaagag
      721 cgccgcggta agcgcgccta tccagatagt cgccttgtcg atagctttca tcgatcctgt
      781 cctttcatcg ccataatcca ctcaagcagg ctctggaaca cggtcgaatt gagctcgtag
      841 tagacgaact gacccttctt ctcccgctcg acgagcccag cgtcagccag catgccgaga
      901 tggtggctca tcgaggcagc gctcatatcg aattctgcgc cgagctcgcc ggcgctcctc
      961 ggacccgcct tgaggagctc gagtatccgt cgcctcgtcg ggtcatcgag agccttccaa
     1021 atcttgttca ccgtgttcgc ggctcctatc gcttagatat ttagaatatc ttctaaatat
     1081 ctaaagacgt caagccgacg attgaggatt ccgcgctagc cgtttcggcg ctatagtaga
     1141 agcgaacgcg ccgaccgctc aaggaggcga cgaatgatcc atactagacg cacgacggcc
     1201 ctagccgtca tccagtttac cttgaccatg ctaccgctcg cggcctggag ccaggcgttg
     1261 ccccgcgatg cctcgaaggc aggtttcatt gcgaccgccg gtaagcgcat ggcttcgttc
     1321 gaagtcaaga gcctcacggg ctcgtcactg agctccaagg acctggctgg ctcgcccgcg
     1381 ctgttccata tctggtccgc gcgcgacgat cctgccggaa acggtccggc cctcgcggcg
     1441 tccttgcgag aagccc
//
LOCUS       DAOOLN010000599         1456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_115732_length_1456_cov_2.186296, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000599 DAOOLN010000000
VERSION     DAOOLN010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115732_length_1456_cov_2.186296"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLW80_07980"
     CDS             <1..231
                     /locus_tag="PLW80_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPB66485.1"
                     /translation="QEGRVRIMAEGREIDLRVSSMPTLLGEKIVVRILDKANLKIRLE
                     DLGFRKETFQAFERMPSMYILLHKRIVDSGKP"
     gene            286..1131
                     /locus_tag="PLW80_07985"
     CDS             286..1131
                     /locus_tag="PLW80_07985"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002682909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPB66486.1"
                     /translation="MREIEAAAITEAVRKLCIESNYVLPADVREALEAGAQAEESPVG
                     RAILADILENADIAAADRVAVCQDTGFALCFVELGQEAAVVGGDFEAAIHEGVRRGYA
                     EGYLRASIVDDPVFERRNTGDNTPAVIHVRVVPGDRIRLTIAPKGGGSENMSAMAMLK
                     PSDGAAGVRRFVVDTVDKAGPNPCPPVIVGVGVGGTIETAALIAKRSLLRAVGERHPD
                     PRVARLEEETLAEINALGIGPQGFGGTVTALDVHIETFPAHIASLPVVVNLQCHAARH
                     LEAVL"
     gene            1128..>1456
                     /locus_tag="PLW80_07990"
     CDS             1128..>1456
                     /locus_tag="PLW80_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HPB66487.1"
                     /translation="MSASGSHSGRLAIRLSLPIVGDDARSLRAGDRVLLSGVLYTGRD
                     VAHRRLVELLDRGGALPFDPSGQVIYYVGPCPAAPGRVIGSAGPTTSGRMDAYAPRLL
                     DAGLRGM"
BASE COUNT          238 a          492 c          504 g          222 t
ORIGIN      
        1 caggaggggc gggtccgcat catggccgag ggccgcgaga tcgacctgcg cgtctcaagc
       61 atgcccacgc tgctcggtga gaagatcgtc gtccgcatcc tcgacaaggc caacctcaag
      121 attcgcctgg aggacctggg cttccgcaag gagaccttcc aggccttcga gcgcatgccc
      181 tcgatgtaca tactgctcca taagcgcatc gtcgactcgg ggaagccgta ggcgattccc
      241 ccgggcgcct tacgtcgcta tactggcgtc gtaaggtggg gcatcatgcg cgagatcgag
      301 gcggccgcta tcaccgaggc ggtcaggaaa ctgtgcatcg agtcgaatta cgttctcccg
      361 gccgacgtgc gcgaggcgct cgaggctgga gcccaggccg aggagtcgcc cgtcggtcgg
      421 gctatcctcg ccgacatact cgagaacgcc gacatagcgg cggcggaccg cgtggccgtc
      481 tgccaggaca ccggcttcgc cctgtgcttc gtcgagctcg ggcaggaggc ggccgtggtc
      541 ggcggcgact tcgaggccgc gatacacgag ggcgtgcgcc ggggctacgc ggaaggctac
      601 ctgcgcgcgt ccatcgtcga cgatcccgtc ttcgaacgca ggaataccgg cgacaatacc
      661 ccggcggtca tccacgtacg ggtcgtgccc ggcgacagga tcaggctgac gatcgcgccc
      721 aagggcggcg gcagcgagaa catgagcgcg atggcgatgc tcaagccgtc ggacggcgcg
      781 gccggcgtca ggcgcttcgt ggtagatacc gtcgacaagg ccggaccgaa tccctgcccg
      841 ccggtgatcg tcggcgtcgg cgtcggcggc accatagaga cggcggccct gatcgccaag
      901 cgctcattgc tgcgagccgt gggcgaacgc catcccgatc ccagggtcgc gaggctcgag
      961 gaggagacgc tcgccgagat caacgctctc ggcatcgggc cgcagggctt cggcggcacg
     1021 gtcaccgccc tcgacgtcca tatcgagact ttcccggccc acatcgcgag cctgccggtc
     1081 gtcgtcaacc tgcagtgcca cgcggcccgc cacctggagg ccgtcctatg agcgcgtccg
     1141 gatcgcatag cggacggctg gcgatccgtc tgagccttcc gattgtcggc gacgacgcga
     1201 ggagcctcag ggccggcgac cgcgtcctgc tgtccggcgt cctctatacc ggcagggacg
     1261 ttgcccacag gcgcttggtc gagctcttgg ataggggagg ggcgctcccg ttcgacccgt
     1321 ccggccaagt catctactac gtgggcccgt gcccggccgc acctggcagg gtcataggct
     1381 cggcaggccc gacgacgagc ggccgcatgg acgcctacgc gccgaggctg ctcgacgccg
     1441 ggctcagggg catgat
//
LOCUS       DAOOLN010000600         1455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_115794_length_1455_cov_3.587857, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000600 DAOOLN010000000
VERSION     DAOOLN010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115794_length_1455_cov_3.587857"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PLW80_07995"
     CDS             complement(<1..818)
                     /locus_tag="PLW80_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HPB66488.1"
                     /translation="MRRSMVVVAILAVSVALILSGCQKSASGTIKIGVAGAHSGDLAS
                     YGLPSVNAAKLVVEAVNAAGGIDGKTVELFIQDDQCKPELATNAAAKLVSDEVVAVIG
                     HICSGATKSALGIYKDSKIITVSPSATTPSLTQSGEYPNFFRTIASDDAQARLATKFI
                     KETLGLSSLAVLHDKGDYGKGFAELVKQYAESEGIRVVLFEGINPGAPDYSAVVNKIG
                     NSGAAGVVWGGYHPEASKLVQQMKDKGMSLPFISDDGVKDQTFIEVAGTYAEGV"
     gene            1067..>1455
                     /locus_tag="PLW80_08000"
     CDS             1067..>1455
                     /locus_tag="PLW80_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB66489.1"
                     /translation="MKLSFGEKASYGVGALGKDMACGIVNTFLMFYFTDVIGLAPAFL
                     GVLFMAARIWDAINDPMAGWLVDNTRGRLGKFRPWILAGTLSNAVVLVVLFSNPGLRG
                     AGQYVFYSVVYILWGMTYTLEDIAYWS"
BASE COUNT          262 a          453 c          446 g          294 t
ORIGIN      
        1 tagacgccct cggcgtaggt tccggccacc tcgatgaagg tctggtcctt gacgccgtcg
       61 tcggagatga acggcaggct catgcccttg tccttcatct gctgaacgag cttggaggcc
      121 tcgggatggt agccgcccca gacgacgccg gcggcgccgg agttgcctat cttattgacg
      181 accgccgagt agtccggcgc gccggggttg atgccctcga agagcacgac ccggatgccc
      241 tcgctctcgg cgtactgctt gacgagctcg gcgaagccct tgccgtaatc gcccttgtcg
      301 tgcagcaccg cgagcgacga gagcccgagc gtctccttga tgaacttggt agccagcctg
      361 gcctgggcgt cgtccgaagc tatggtcctg aagaagttag ggtactcgcc gctctgggtc
      421 agcgacggcg tggtcgcgga cggggagacg gtgatgatct tcgaatcctt atagatgccg
      481 agcgccgact tggtggcgcc ggagcagata tggccgatga cggcgacgac ctcgtcgctg
      541 acgagcttgg cggcggcgtt ggtcgccaac tcgggcttgc actggtcgtc ctggatgaac
      601 agctcgaccg tcttgccgtc gatgccgccg gccgcgttca ccgcctcgac gaccagcttg
      661 gccgcgttca ccgaaggcag gccgtaggaa gccaggtcgc ccgagtgcgc gccggccacg
      721 ccgatcttga tggtcccgct cgcgctcttc tggcagccac taagaatgag cgcgactgat
      781 actgccagga tcgccactac caccatgctt cttctcattc gttcctccta atcagctgcc
      841 gcatcgtacg tttatgacca ccgcggtcat cgcgccgacg aagcgccagg tacaagtgct
      901 cctatcgttt ctatgtatag tagtagcgtc gccttagtca agtagcgaat gcctatgcat
      961 agaatcaaac atgccaggag tcggcgcaag gtacgcattg acgatacgac cggccgcgcc
     1021 gatactgttc aggccgcccg gatcgcgcgg ccttcaagga gactgcatga aactgtcttt
     1081 cggagaaaaa gcgagctacg gcgtcggcgc gctcggcaag gatatggcct gcggcatcgt
     1141 caacaccttc ctcatgttct attttacaga cgtgataggg ctggccccag cgttcttagg
     1201 cgttttgttc atggcggcca gaatctggga cgcgatcaac gacccgatgg ccggctggct
     1261 ggtcgataac acgagaggcc ggctcggcaa attcagaccc tggatactcg ccggcacgct
     1321 gagcaacgcc gtggtcctcg tcgtgctgtt ctcgaacccg ggcctccgcg gcgccggcca
     1381 gtacgtcttc tacagcgtcg tctacatact ctggggcatg acctacacgc tcgaggacat
     1441 agcgtactgg tcgat
//
LOCUS       DAOOLN010000601         1454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_115936_length_1454_cov_2.858470, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000601 DAOOLN010000000
VERSION     DAOOLN010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115936_length_1454_cov_2.858470"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PLW80_08005"
     CDS             <1..639
                     /locus_tag="PLW80_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB66490.1"
                     /translation="YTLGKEGYETTRAATGEAGLSAMRERAPDLVLLDVMLPGMDGFE
                     LLRKAKADPALARVPVLMVSARGEEADVVTGLELGAEDYVTKPFSPRVLVARARAALR
                     RSGDRAGDTPEDGPVVRGPCAIDPARHELLIDGRPVDLSATEFAMLELLMRESGRVFT
                     RAQMLDRVRGHDYPVTDRAVDVQILSIRRKLGRHAELVETIRGVGYRFKDLP"
     gene            636..>1454
                     /locus_tag="PLW80_08010"
     CDS             636..>1454
                     /locus_tag="PLW80_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66491.1"
                     /translation="MRRARPGTRTFTALFLLSAAAVAIMAASSVVAMRRLQRDSTLSA
                     LSQAATALANALPGALSGARLQGSAGLAPFAAAVDGFCDAAAAGTSLRVSAIAADGSV
                     LGDSGSDPGKMDNHATRPEFAEALLGRPTTIIRRSATLGLEMAYAAAPIRYGGSVAGA
                     LRVAMGAPDLGDELAPFLALSLALATATIAATAVASRRIGAAITMPLRALVEAAGDWS
                     AGCLDRRVRHSGDPAFEPLADAMNAMAAELAARTASTERQQRELGAILDAMGEAV"
BASE COUNT          199 a          525 c          524 g          206 t
ORIGIN      
        1 tacaccctcg gcaaggaggg ctacgagacg acgcgggccg cgaccggcga ggcgggcctc
       61 tccgcgatgc gggagcgcgc gcccgacctc gtcctgctcg acgtgatgct acccggcatg
      121 gacggcttcg agctgttgcg caaggccaag gcggacccgg ccctggccag ggtgccggtg
      181 ctgatggtca gcgcgcgcgg cgaggaggcg gacgtggtga ccggcctcga gcttggcgct
      241 gaggactacg tgaccaagcc gttcagcccg cgcgtgctcg tggcgcgggc cagggcggcc
      301 ctcaggcgct cgggcgaccg ggccggagat acgcccgagg acggccccgt agtccgaggc
      361 ccctgcgcca tcgaccctgc caggcacgag ctgctcatcg acggccggcc ggtcgacctg
      421 tcggcgaccg agttcgcgat gctcgagctt ttaatgcgcg agagcggccg cgtgttcacc
      481 cgcgctcaga tgctcgaccg ggtgcgcggc cacgactacc cggtgaccga ccgcgccgtc
      541 gacgtgcaga tcctatcgat acgccgcaag ctcggccgcc atgcggagct cgtcgagacg
      601 atacgcggcg tcggctaccg cttcaaggac ctgccgtgag gcgcgcccgc ccggggacga
      661 ggaccttcac ggcgctgttc ctcctatcgg cggccgcggt cgcgattatg gcggccagct
      721 cggtagtcgc catgcgccgc ctgcagcgcg actcgaccct gtcggcgctg tcccaggcag
      781 ccaccgcgtt ggccaacgcc ttgcccggcg cgctgtccgg ggcccggtta caaggaagtg
      841 ccggtttggc gcccttcgcg gcggccgtgg acggcttctg tgacgcggcc gcggccggca
      901 cgagccttcg cgtctcggcc attgccgccg atggctcggt gctcggcgac tcgggctccg
      961 accccggcaa gatggataac cacgcgacca ggccagaatt tgccgaggcc ctgctcggta
     1021 ggcctaccac gatcatcagg agatcggcga cactgggcct cgagatggcc tacgccgccg
     1081 ccccgatacg atacggaggg tcggtagccg gcgccctccg ggtggcgatg ggcgcgcccg
     1141 accttggcga cgagctggcg cccttcctag cgctgtcgct cgcgttagcg actgctacca
     1201 tagccgccac ggcggtcgcg agccgacgca tcggcgcggc gatcaccatg ccgctccgcg
     1261 ccctcgtcga ggccgccggc gactggtcgg cgggctgcct cgatcgccgc gtccggcatt
     1321 ccggcgatcc ggccttcgag ccgctcgccg acgcgatgaa cgcgatggcc gccgaactgg
     1381 cggcgcggac ggcttcgacc gagcggcagc agcgcgagct cggggccata ctcgacgcga
     1441 tgggcgaggc ggta
//
LOCUS       DAOOLN010000602         1454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_115960_length_1454_cov_2.454610, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000602 DAOOLN010000000
VERSION     DAOOLN010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115960_length_1454_cov_2.454610"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1454
                     /locus_tag="PLW80_08015"
     CDS             <1..>1454
                     /locus_tag="PLW80_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66492.1"
                     /translation="GAGFVEYSGSLYGRHPMRRQRAIYNDLKPVISTCRFSLIRNTAL
                     VTQLLTTSADIPVRILDGEAPYFVGYVRPTFKATVNGSHGLEAVEVEAVDLWYKLDRT
                     VGTTRTINGWAVSNPSNKAASLLHRIFYDAGIADSELSLAAVTATVPSITLTAGSGTY
                     RSIVEQILAEAGYSARVSADGVVSLYDLGPSTITPVLTLSSGVSGNIASGYKLERKEA
                     TAEAVDVEYHITKALTDVMLFEDTTGATAGLDASISVPAGTYYPEGAASGKSVKAAMR
                     IDGYDLVSASDITSDIAYNGALTIEEFTGEGKNILMRLYSASGAVITRLRGYGDAIVK
                     GDLRRVVRENVASTTKREKVEFKYLADDTSAARVARIRAARHLHGAYRWSMTSTELSL
                     TPGDYVSLNESSVLGTSTTLRIVSVIDGPDPKTVSIEAEGVAEYTADAVETETETNQP
                     PSPSPAAEAREAAMKSAIELAQKTLTLSAPAISR"
BASE COUNT          270 a          508 c          453 g          223 t
ORIGIN      
        1 tcggcgccgg gttcgtcgag tattcgggct cgctctatgg ccgccatccg atgcgccggc
       61 agcgcgcgat ctacaacgac ctcaagccgg tcatctcgac ctgccggttc tcgctgatcc
      121 gtaacacggc gctcgtcacg cagctcctaa cgacgagcgc ggacattccg gtccggatcc
      181 tcgacggaga ggccccgtac ttcgtcggtt acgtccgccc gaccttcaag gccacggtca
      241 acggatcgca cgggctcgag gccgtcgagg tcgaagcggt cgacctctgg tacaagctcg
      301 accgcaccgt cggcacgacg cgaacgatca acggctgggc cgtatccaac ccgagcaaca
      361 aggccgcctc actactgcac cggatcttct acgacgcggg catagccgac tcggagctgt
      421 cgctcgccgc cgtgactgcg acggtaccgt cgatcacgct gaccgccggg agcggcacgt
      481 atcgctccat cgtcgagcag atcctcgcgg aggccggcta ctcagcgcgc gtgagcgcgg
      541 acggcgtcgt gtcgctgtac gacctcggcc cgtctaccat cacgcccgtg ctgacgctgt
      601 cctccggagt aagcggcaat atcgcatcag ggtataagct cgagcgcaag gaggcgaccg
      661 ccgaggccgt cgacgtcgag tatcacatca cgaaggcgct gaccgacgtc atgctcttcg
      721 aggacacgac cggggcgacc gctggcctcg atgcgtcgat ctccgtgccg gccgggacgt
      781 actacccgga gggcgccgcg tccgggaagt ccgtgaaggc cgcgatgcgg atcgacggct
      841 acgacctcgt ctccgcgtcg gacatcacct cggacatcgc ctacaacggc gcgctgacca
      901 tcgaggagtt caccggtgaa ggcaagaaca tcctcatgcg gctgtactcc gcgtccggcg
      961 cggtgattac tcggctacgc gggtacggcg acgccatcgt caagggcgac ctacggcgcg
     1021 tcgtgcgcga gaacgtggct agcacgacga agcgcgagaa ggtggaattc aagtaccttg
     1081 ccgacgacac gagcgccgct cgcgtcgcca ggatccgcgc ggcccggcac ctgcacggcg
     1141 cgtaccgctg gtctatgacc tcgaccgagc tatcattgac gcccggcgac tacgtgtcgc
     1201 tgaacgagag ctccgtcctg ggcacatcga cgacgctccg tatcgtctcc gtgatcgatg
     1261 gccctgatcc gaagacggtc agcatcgagg ccgagggcgt cgccgaatac accgccgatg
     1321 cggtagagac cgagaccgag acgaaccagc cgccaagccc gagccccgcg gccgaagccc
     1381 gcgaggcggc tatgaagtcc gccatcgagc tggctcagaa aacccttacc ctatccgccc
     1441 ccgcaatctc acga
//
LOCUS       DAOOLN010000603         1454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_115986_length_1454_cov_2.080057, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000603 DAOOLN010000000
VERSION     DAOOLN010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115986_length_1454_cov_2.080057"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PLW80_08020"
     CDS             <1..764
                     /locus_tag="PLW80_08020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="spiro-SPASM protein"
                     /protein_id="HPB66493.1"
                     /translation="GLEGFMPVERFERLMADAEALCDDLVVDLSLWGEPSAHPDFPAL
                     ARAVLARPRFTLIVETSGVGWERGQAESLAAEAGSRAQWVISLDAVDDEGYLALRGAG
                     KDEAAAFAERLIAASPGNVHVQAVRMKENEAGLEAFYRGWKQRGAKGIVQKYDSFAGS
                     LPDRSVADLSPLERLPCRHLARDMAVLLDGSVPWCKHCLVPGERLRYAGFAGNAFSDG
                     LGSTWAAMGAWYDRHISGELPEPCGRCDEYHTFNA"
     gene            739..>1454
                     /locus_tag="PLW80_08025"
     CDS             739..>1454
                     /locus_tag="PLW80_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66494.1"
                     /translation="MNTIPLTLDAYKPTYTVLGGGRGSGCRDGFPVSVLLLNRGPMLY
                     RAEMIQELVRVGFESIVSMEGIGDSPELEGLASRYPQVRFICLHETANLGVRVNIGMR
                     ESCSPFVFVLWNDQRLATSTLSSRFFDKVVDLDAACLVPTLNDASGSPVPSISHPAQS
                     GKAFRIVPLPPKADGEKSLYPFDACGIYSREKFMLLGGFDWTIDNPYWQKLDFGMRTW
                     LWGETIRYAQALRLNYDGAPP"
BASE COUNT          228 a          482 c          479 g          265 t
ORIGIN      
        1 gggggctcga gggcttcatg ccggtcgagc gcttcgaacg gctgatggcc gacgccgagg
       61 ctctctgcga tgatctggtc gtcgatctgt ccctctgggg cgagccctcg gcgcacccgg
      121 atttcccggc gctggctcgg gccgtcttgg cgcgcccgcg cttcacgctg atcgtcgaga
      181 cttcgggcgt cggctgggag cggggccagg ctgaatccct ggccgccgag gccggctccc
      241 gtgcccagtg ggtcatctcc ctcgacgcgg tcgacgacga gggctacctg gcgttgcgcg
      301 gagcgggcaa ggacgaggcc gcggctttcg ccgagcgctt gatcgcagcc tcgcccggca
      361 acgtccacgt ccaggcggtg cgcatgaagg agaacgaggc cggactcgag gccttctacc
      421 ggggctggaa acagcgaggg gccaagggga tagtgcagaa gtacgactcc ttcgccggaa
      481 gcctccccga tcggagcgtc gccgacctat cgccgctcga gcggcttccc tgccgccacc
      541 tggcccgcga catggccgta ctgctcgacg gctcggtacc ctggtgcaag cattgcctcg
      601 tgcctggcga acgcctacga tacgcaggct tcgccggtaa cgcgttctcc gacggcctcg
      661 gctcgacctg ggcggctatg ggtgcctggt acgataggca tataagcggc gagctgcctg
      721 aaccatgcgg acggtgcgat gaataccata cctttaacgc ttgacgccta taagcccacc
      781 tacaccgtgc tcggcggcgg ccgaggctcc ggttgcaggg acggctttcc cgtgtcggtg
      841 ctactcctga atcgcgggcc gatgctatat cgcgccgaga tgatccagga gctggtgcgc
      901 gtcggcttcg agtcgatcgt gtcgatggag ggtattggcg attctcccga gctagaaggc
      961 ttggcgtcgc gctatcccca ggtccgcttc atctgcctgc acgagaccgc gaacctcggc
     1021 gttcgcgtga acatcggcat gcgtgaatcg tgctcgccct tcgtcttcgt gctatggaac
     1081 gatcagcgcc tggccacgag cacgctgtcg agccgcttct tcgacaaggt ggtcgacctg
     1141 gacgcggcct gcctcgtgcc gaccctcaac gacgcctcgg gctcgcccgt gccgtcgatc
     1201 agccatcccg cccagtctgg caaggccttc aggatagtgc ccttgccgcc gaaagccgat
     1261 ggcgagaaga gcctgtaccc cttcgatgcg tgcggtatct actccaggga gaaattcatg
     1321 ctgctcggcg gcttcgactg gaccatcgac aacccgtact ggcagaagct cgacttcggc
     1381 atgcggacct ggctctgggg agagacgata cgctacgcgc aggcgctgcg attgaactac
     1441 gacggcgccc cgcc
//
LOCUS       DAOOLN010000604         1451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_116335_length_1451_cov_2.806590, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000604 DAOOLN010000000
VERSION     DAOOLN010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116335_length_1451_cov_2.806590"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLW80_08030"
     CDS             <1..290
                     /locus_tag="PLW80_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HPB66495.1"
                     /translation="DLDSYSAFYENDRSTPTGLDGYLRALGASAVVVAGLALDYCVYY
                     SAFDAARLGYSVVVVSDATRAVGSPKGSVAAALADLRSRGVAIIDSLEVFR"
     gene            287..>1451
                     /locus_tag="PLW80_08035"
     CDS             287..>1451
                     /locus_tag="PLW80_08035"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002673136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HPB66496.1"
                     /translation="MNSSALFTDFYELTMAQGYWKHGLDTSAVFDVFFRRQPFKGGFS
                     VFAGLEPLLEALEVFRFSQDDLAWLSAQPGFDPAFVAYLADFRFSGDIYAMPEGELAF
                     PQEPLLRVHANLIEAQIIEGLVLNTINFQSLIATKSARVALAAKGKKVMEFGLRRAQG
                     FDGAMSASRAAYIGGAHGTSNALAGKRLGIPALGTMAHSWVMAFESELASFEAYADMY
                     PDGTTLLIDTFDTLGSGLPNAIKVGNRLAGRGKKLGVRLDSGDIDYLSRKVREGLDEA
                     GLDDAFIVVSNELDEEIIETLVAAGAPVDVWGVGTHLVTGGDEASFTGVYKLAAFERG
                     GVWSPTMKFSDNPDKSTNPGVKDLYRVYDESGAAVADVMTLAGETIEPGKVATF"
BASE COUNT          239 a          487 c          468 g          257 t
ORIGIN      
        1 cggacctgga cagctactcg gctttctacg agaacgaccg atccaccccg accgggctcg
       61 acggctacct gcgcgccctc ggcgcctcgg ccgtcgtcgt cgcggggctg gccctcgact
      121 actgcgtata ctacagcgcc ttcgacgctg cccgtctcgg ctactcggtc gtcgtcgtct
      181 ccgacgccac cagggccgtc ggctcgccga agggttccgt cgccgccgcg ctcgctgacc
      241 tcaggtccag gggcgtggcg atcatagatt ccttggaggt tttccgttga actcgagcgc
      301 cctattcacc gatttctatg agctgacgat ggcccagggc tattggaagc acggcctgga
      361 taccagcgcc gtcttcgacg tcttcttccg gcgacagccc ttcaagggcg gcttctcggt
      421 gttcgccggc ctggagccgc tcctcgaggc gctcgaggtc ttcaggttct cccaggacga
      481 cctggcctgg ctgtcggcgc agcctggctt cgacccggcc ttcgtcgcgt acctggcgga
      541 cttcaggttc tccggagaca tctacgcgat gcccgagggc gagctcgcct tcccgcagga
      601 gccgctcctg cgcgtccatg ccaatctgat agaggcgcag atcatcgaag gcctggtgct
      661 caatacgatc aatttccaga gcctcatcgc gacgaaaagc gccagggtcg ccctggcggc
      721 caagggcaag aaggtgatgg agttcgggct caggcgggcc cagggcttcg acggagcgat
      781 gtccgcctcg cgggcggctt atataggcgg cgcccacggt acctcgaacg cgttggccgg
      841 caagcggctc ggcatcccgg ccctcggcac gatggcccat agctgggtga tggccttcga
      901 gagcgagcta gcctcgttcg aggcttacgc cgacatgtac ccggacggca ccaccctgtt
      961 gatcgacacc ttcgacacgc tcggctccgg cctgccgaac gcgatcaagg tcggcaatcg
     1021 cctggcaggg cgaggcaaga agctaggcgt caggctcgac tccggcgata tagactacct
     1081 gtcgcgcaag gtacgcgagg ggcttgacga ggctggcctc gatgacgcct tcatcgtcgt
     1141 atctaacgag ctcgacgagg agatcatcga gacgctcgta gccgccggcg cgccggtcga
     1201 cgtctggggc gtcggtaccc acctggtcac cggaggcgac gaggcatcct tcaccggcgt
     1261 ctacaagctg gccgcgttcg agaggggcgg cgtatggtcg ccgacgatga agttctcgga
     1321 caaccccgac aagtcgacca accccggcgt caaggacctg taccgcgtct acgacgagtc
     1381 gggcgccgcc gtggccgacg tgatgacgct ggccggcgag acgatagagc ccgggaaggt
     1441 agctaccttc c
//
LOCUS       DAOOLN010000605         1448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_116674_length_1448_cov_4.343144, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000605 DAOOLN010000000
VERSION     DAOOLN010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116674_length_1448_cov_4.343144"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..948)
                     /locus_tag="PLW80_08040"
     CDS             complement(79..948)
                     /locus_tag="PLW80_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB66497.1"
                     /translation="MTNQALAANVRARRLRAAATVLQYALAIAIVLIVVFPLYWMIIS
                     SLKTSEELLQAVPSLWPAEFRWRNYADALAMAPFGKYFFNTVVMTVGIMGLQLVIGSF
                     AAYGFAKGRFPGRDALFIVVLGALMIPIQVTFVPIYGMVAKLHWINTFAGLIVPHAVS
                     AYMIFLLRQTFKSVDDSFLEAARLEGMGRVGVIFRILVPMCKPTMMTVGIISFIDGWN
                     AYFWPKMVTTTSFRRTIAQGVYELKRSYAGLETMNQNQIMAGAVMAVVPIVVLFVVLQ
                     KYILSGFSKAASK"
     gene            complement(961..>1448)
                     /locus_tag="PLW80_08045"
     CDS             complement(961..>1448)
                     /locus_tag="PLW80_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB66498.1"
                     /translation="FLNYVLGAFGVGPVAWLNSERTALTSVLILTGWRVVGYGMMIYL
                     SAMKGISKDYYEAAELDGAGAVQRFRHVTLPLLSPTTLFLFVTTFIASMKVFQSIDVM
                     TAGGPYESTNVMVYWIYDLAFVQFRVDRAAVVASVFFVILLAFTAATMKISNKSVHYD
                     S"
BASE COUNT          287 a          424 c          500 g          237 t
ORIGIN      
        1 cggcgccgcg aagcggcggc gcgccgcatg gcgcgccgaa aggccggcct tatagtcgat
       61 ttcgcacatc attcggcctt acttggaggc ggccttggag aagccgctca ggatatattt
      121 ctggagcacg acgaacagta cgacgatggg cacgacggcc atcacggcgc cggccatgat
      181 ctggttctgg ttcatcgtct cgagcccggc gtaggagcgc ttgagctcgt agacgccctg
      241 ggcgatggtg cgccggaagg aggtggtggt gaccatcttg ggccagaagt acgcgttcca
      301 gccgtcgatg aacgagatga tgccgacggt catcatggtc ggcttgcaca tgggcacgag
      361 tatcctgaag atgacgccga cgcgtcccat gccctcgaga cgggcggcct cgaggaagct
      421 gtcgtcgacc gacttgaagg tctgcctgag gagaaagatc atgtaggccg agacggcgtg
      481 cggcacgatg aggccggcga aggtgttgat ccagtgcagc ttggcgacca tgccgtagat
      541 cggcacgaag gtcacctgga tcggtatcat gagggcgccg agcacgacga tgaacagggc
      601 gtcgcggccg gggaaccggc ccttggcgaa gccgtaggcg gcgaacgagc cgatgaccag
      661 ctggaggccc atgatgccga ccgtcatcac gacggtattg aagaagtact taccgaaggg
      721 cgccatcgcg agcgcgtcgg cgtagttccg ccagcggaat tcagccggcc agagcgatgg
      781 cacagcctgg agcagctcct cgctggtctt gagcgacgat atgatcatcc agtagagcgg
      841 aaagaccacg atcagcacga tcgctatcgc gagcgcgtac tggaggacgg tggcggcggc
      901 cctgagccgc ctggcgcgga cgttcgccgc gagagcttgg ttcgtcatcg ccgttccctc
      961 ctaggagtcg taatggacgc tcttgttcga tatcttcatc gtcgcggccg tgaaggcgag
     1021 caggatgacg aagaacacgc tggcgacgac ggcggcccgg tcgacgcgga actggacgaa
     1081 ggccaggtcg tagatccagt agaccatcac gttcgtgctc tcgtagggcc cgccggcggt
     1141 catcacgtct atcgactgga agaccttcat cgacgcgatg aaggtggtga cgaacaggaa
     1201 cagcgtcgtc ggcgacagga gcggcagggt gacgtgcctg aagcgctgca cggccccggc
     1261 cccgtcgagc tcggcggcct cgtagtagtc cttggagatg cccttcatcg ccgacaggta
     1321 gatcatcatg ccgtagccga cgacgcgcca gccggtcagg atcagcaccg aggtcagggc
     1381 ggtccgctcc gagttgagcc acgcgaccgg cccgactccg aaggcgccga gcacgtagtt
     1441 caggaagc
//
LOCUS       DAOOLN010000606         1448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_116710_length_1448_cov_2.732950, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000606 DAOOLN010000000
VERSION     DAOOLN010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116710_length_1448_cov_2.732950"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1326
                     /locus_tag="PLW80_08050"
     CDS             <1..1326
                     /locus_tag="PLW80_08050"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB66499.1"
                     /translation="PGSMGRICHFHCQMRCRRDTLDEPVAQGEIHRYLADTMYKMGRE
                     KDVWAKLVKERKPPTGRSIAIVGSGPAGLAAGFYLSRLGHEVTVFESKPKAGGILRYG
                     IPTYRLPKDVLDKELELWKRLGVKFVYNTMVGRDVGFDSLRSQYDALIVAIGAQADKR
                     LGIPGEELDGVHAGYAWLEAFSMGKATRRGRRALVIGGGNVAIDAARTLFRLDCDVTV
                     AYRRAKQSMPANVVELAGAEEEGIRFEYMLQPLEALGDGGRVKAVRFRVMRPGGMDSS
                     GRPKPVETDRTVDIPCDDVYVAVGERVDSSFVASDGVTTTSDGWIETDRLTGKASGSV
                     WAIGDAVTGPATAAEAMGLGKRVAKAIDAELMGREAFGELFKDLGHSMAVPPEPVKTG
                     MYRSRLVPPEERRGNFQEISQGYSGDQALLECARCLRCDIRADARSPWR"
BASE COUNT          272 a          465 c          506 g          205 t
ORIGIN      
        1 cccggctcga tggggcgcat ctgccacttc cactgccaga tgcgctgccg ccgcgacaca
       61 ctcgacgagc cggtagccca gggcgagatc caccgctacc tggccgacac gatgtacaag
      121 atggggcggg agaaggacgt ctgggccaag ctcgtcaagg agaggaagcc gcccacggga
      181 cgctcgatag ccatcgtcgg ttcgggcccc gcgggcctcg ccgccggctt ctacctgtcc
      241 aggctcggcc acgaggtcac ggtcttcgag tccaagccca aggccggcgg catactgcgc
      301 tacggcatcc cgacctaccg gctacccaag gacgtgctcg acaaggaact ggagctatgg
      361 aagcggctcg gcgtcaaatt cgtgtacaac acgatggtgg gccgggacgt gggcttcgat
      421 tcgctacgca gccagtacga cgcgctgata gtcgccatag gcgcccaggc ggataagcgc
      481 ctcgggatac ccggcgagga gctcgacggc gtacacgccg gctatgcctg gctcgaggca
      541 ttctcgatgg gcaaggcgac gaggcgaggc cggcgcgccc tggtcatcgg cggcggcaac
      601 gtcgccatcg acgcggccag gactcttttc aggctggact gcgacgttac cgtggcctac
      661 cggcgcgcca agcagagcat gccggccaac gtggtcgagc tggccggcgc cgaggaggaa
      721 ggcatccgct tcgagtacat gctgcagccc ctcgaggccc tcggcgacgg agggcgcgtc
      781 aaggcggttc gcttcagggt aatgcggccg ggaggcatgg atagtagcgg ccgacccaag
      841 ccggtcgaga ccgaccggac cgtggacata ccctgcgacg acgtctacgt ggcggtcggc
      901 gaacgggtgg actcctcgtt cgtcgcctcc gacggcgtta caaccaccag cgacggatgg
      961 atcgagaccg acaggctgac cggcaaggcc tcgggctccg tatgggcgat aggcgacgcc
     1021 gtaaccgggc cggccacggc ggccgaggct atgggtctcg gcaagcgggt cgccaaggcg
     1081 atcgacgccg agctcatggg ccgcgaagcg ttcggcgagc tattcaagga cttaggccac
     1141 tcgatggccg taccgccgga gccggtcaag acgggcatgt accgctcgag gctcgtgccg
     1201 ccggaggaac gccggggcaa tttccaggag atatcccagg gatactccgg agaccaggcc
     1261 ctgctcgagt gcgcgcgctg cctgcgctgc gacatcaggg ccgacgcccg tagcccatgg
     1321 cgatgatcgg aggatagcat ggatggaacc aaagccaaga cccttagcct atcgatcgac
     1381 ggccgagagc tgagcgtacg cgaagggacg acgatactcg aggcctgccg cggtgctggc
     1441 gtcaggat
//
LOCUS       DAOOLN010000607         1447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_116865_length_1447_cov_1.957615, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000607 DAOOLN010000000
VERSION     DAOOLN010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116865_length_1447_cov_1.957615"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLW80_08055"
     CDS             complement(<1..287)
                     /locus_tag="PLW80_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66500.1"
                     /translation="MYSLGPGQFYIRKRLEIDPGEHLLNWVDVESLRLAMVAITGSKE
                     PCQQHRPSRVAESVSDLEEGYCREPNRRGPSRARDRHGIVDVGRAGAKEFWA"
     gene            complement(383..715)
                     /locus_tag="PLW80_08060"
     CDS             complement(383..715)
                     /locus_tag="PLW80_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66501.1"
                     /translation="MRRQAVLPYEGPQPNAGLGTELYYGSYAQTYLERDIDPDGQAVD
                     IAAPSSRPGASARSTFYSITSYLLHHYLAVFLVSTLTYGFTTAAMMMPALYEFDIAMG
                     RSKTQGDE"
     gene            1062..1268
                     /locus_tag="PLW80_08065"
     CDS             1062..1268
                     /locus_tag="PLW80_08065"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HPB66502.1"
                     /translation="MRQVIVYPGEDGYYVAECPSLPGCISQGASKETAIENIKEAIQA
                     YTLSLQGQGLSIPVDRFDSILVAV"
BASE COUNT          303 a          418 c          407 g          319 t
ORIGIN      
        1 gcccaaaatt cctttgcccc cgctctccca acatcgacga ttccgtgtcg atctctcgcc
       61 cgtgaaggcc ctcgccggtt cggctctcgg cagtaacctt cttccagatc gctgacagac
      121 tcggcaactc gactcggtcg gtgctgctga cacggctctt ttgagcccgt gatcgccacc
      181 atggccagac gcagcgattc cacgtccacc cagttcagca ggtgctcgcc ggggtcgatt
      241 tccagccgct tgcggatata gaactggccg ggccccagcg agtacacgac cgcggcgccg
      301 cattggccga cacggccgcg agcagacagg aaacgacagc caacaggcag tcggcgatca
      361 tgacgtgtct ccggcgctcc gctcactcgt ccccctgcgt cttggagcgt cccatggcga
      421 tgtcgaattc gtacagggcc ggcatcatca tcgcggcggt cgtgaaaccg taggtcaagg
      481 tggacacgag gaacacggcc aggtaatggt gcagcagata gctcgtaatc gaatagaagg
      541 tcgatctcgc cgacgcacca ggtctcgaag atggagcagc gatatccact gcttgaccgt
      601 cgggatcgat atcccgctcc aggtaggtct gcgcgtacga gccgtagtag agctccgtac
      661 cgagccccgc gttcggctga ggcccttcat agggcaggac ggcttggcgt cgcaccggag
      721 cttgatcgcc ggtaggagct gaggcgcgta gcgctgcagg aagcgctccg ggtcttcgct
      781 ggccagcatg cggaccggca tcgagtcgag cgagacgtag cgccgcgcct cttccttgac
      841 gcggaggagc acggtcgtct tgcccgtctg cctgggtccg gtaacgagga tggcggggaa
      901 gtgagacgag gtctctctac tatcttgctc gcctttaccc tattcttatg cgccttggcg
      961 ccttagtgtg aggtcctcaa tactatgcat catatgttct ctatccgatt ctcctatatt
     1021 tttaaccggt tgttagtatt attcatctca ggaggatcga tatgcgtcaa gtaatcgtat
     1081 atcccggtga ggatggctac tatgtcgccg aatgtccaag cctacctggt tgcatttctc
     1141 agggagcctc gaaagaaaca gcaatagaga atatcaaaga agcaattcag gcgtatacgc
     1201 tttcgcttca ggggcaaggt ctttctattc cagtcgatag gtttgattcc atccttgtag
     1261 ccgtatgagc catctaccta gaatatctgg tagattctgc gttgctgcgc tacagagagc
     1321 gggatttgaa gtactacgac agcatggcaa ccatatcgta ctaaagcgca atgagccatt
     1381 cgtccagcta gtgattcccc gatcataaag aactggatac cgggacgcta cgagcgataa
     1441 tcaggca
//
LOCUS       DAOOLN010000608         1445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_117080_length_1445_cov_2.422302, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000608 DAOOLN010000000
VERSION     DAOOLN010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117080_length_1445_cov_2.422302"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1445)
                     /locus_tag="PLW80_08070"
     CDS             complement(<1..>1445)
                     /locus_tag="PLW80_08070"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPB66503.1"
                     /translation="SVFAQTAAQESGLEAPAPAASPADPFAAGYEAAVAAYRAAIDFS
                     APFEPVGDLPSPGTAVGDGVAPWSLDELLALAAGRNPGLRALAEAEAAAAADLAGARA
                     RRLPTLRAETSGTYIGNPMGPIALTAGQLGAFQGVSIPPEDVIIYKGMESYNYDFALI
                     GEVPLYTWGKIAIGVDLARAGLDAAGLQRRKAERELAARMRGDWDALACLRAADEMLG
                     LQATIGRRLVELAEASAEAGFMTRADLAGARIKLKEIDIARVRLDERRDKLLAELASM
                     AGLDALSKDDLEMTPPIAGSPRWTEAEAGALALGNSYDLALLEAQLVARRGLRDLAEA
                     EAKGRPDIGLRVELSYGGSRLPFVEKDWFGQDDYQLTFSVGTSGNIFGNAVKEGEAAK
                     ARALLAEAAAQRADAERAIRSFVRDTWLGMELGRARLEYAALRQDGWASDLAQRRAAL
                     RAGGGSESEYLTAMIEALGSLAEAYGSLAEYR"
BASE COUNT          197 a          561 c          477 g          210 t
ORIGIN      
        1 cggtactcgg ccaggcttcc gtaagcctcg gcgagcgagc cgagcgcctc gatcatcgcg
       61 gtcaggtact ccgactccga cccgccgccg gcccgcaggg ccgcccgtcg ctgcgccagg
      121 tcggaggccc agccgtcctg cctgagcgcc gcgtactcga gcctggcgcg gcccagctcc
      181 atgcccagcc aggtatcgcg cacgaaggac cggatggccc gctcggcgtc ggcccgctgc
      241 gcggcggctt cggccagcaa ggcccgagcc ttggcggcct cgccctcctt gaccgcgttc
      301 ccgaagatat tgcccgaggt gcccacgctg aaggtcaact gatagtcgtc ctgcccgaac
      361 cagtccttct cgacgaaggg caggcgcgag ccgccgtacg acagctccac gcgcaggccg
      421 atgtctggcc ggcccttcgc ctcggcctcg gccaggtcgc gcaggccgcg cctggccacg
      481 agctgcgcct cgagcaaggc caggtcgtag ctgttcccga gggccagcgc gccagcttcc
      541 gcctcggtcc agcgcggcga gccggcgatc ggcggagtca tctccaggtc atccttcgat
      601 agagcgtcga ggccagccat cgacgcgagt tcggcgagca atttgtccct acgctcgtcg
      661 agacgcactc tggctatgtc aatctccttg agcttgatgc gcgcgccggc caagtcggcc
      721 cgggtcatga agccggcctc ggcgctcgcc tcggccagtt cgacgaggcg tcgcccgatc
      781 gtagcctgta ggccgagcat ctcgtcggcc gcccgcaggc aggccagcgc gtcccagtcg
      841 ccccgcatcc tggcggccag ctcgcgctcg gccttgcgcc gctgcaggcc agcggcgtcc
      901 aggcccgccc gggccaggtc caccccgatg gctattttgc cccaggtgta cagcggcacc
      961 tcccctatca gcgcaaaatc atagttgtag ctctccatcc ccttgtagat gatcacgtcc
     1021 tcgggcggta tcgagacgcc ctggaacgcg ccgagctggc cggcggtcag cgcgataggc
     1081 cccatggggt ttccgatata cgtgccggag gtctccgccc tgagggtcgg caggcgcctc
     1141 gccctcgcgc ccgccaggtc cgccgcggcc gccgcctcgg cctcggcgag cgcccgcagc
     1201 ccgggatttc gcccggcggc caacgccagt agctcgtcga gcgaccaggg cgcgaccccg
     1261 tcgccgaccg ccgtccccgg ggacggcagg tcgccgaccg gctcgaacgg cgccgagaag
     1321 tcaatcgccg cccggtacgc cgctaccgcc gcctcgtagc ccgccgcgaa cggatcggcc
     1381 ggagacgccg cgggcgcggg cgcctcgagg ccgctctcct gcgccgcggt ttgtgcgaat
     1441 acaga
//
LOCUS       DAOOLN010000609         1444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_117221_length_1444_cov_2.426206, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000609 DAOOLN010000000
VERSION     DAOOLN010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117221_length_1444_cov_2.426206"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="PLW80_08075"
     CDS             complement(<1..911)
                     /locus_tag="PLW80_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPB66504.1"
                     /translation="MYEPVDPKASFPSMEESVLAFWNDRDVFKRSISERDGSPEYVFY
                     DGPPFATGLPHFGHFVPGTIKDVIPRYKTMKGFKVERRFGWDCHGLPVENLIEKELGL
                     DSKTDIERFGVANFNEACRASVQRYVKEWRTIMTRAGRWVDFDNDYKTMDPDYMESIW
                     WVVKNLWDQGLVYEGHYILPYCPRCSTVLSNHELALGGYKDVHDPAITIKFKVLEAPA
                     GLEGLADGSTYLLAWTTTPWTLPSNLALCLGPDIDYVLVRDGAERYLLAESRLGAYYK
                     EGQQPEILWRKKGSELVGIRYEPLFPY"
     gene            1161..>1444
                     /locus_tag="PLW80_08080"
     CDS             1161..>1444
                     /locus_tag="PLW80_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66505.1"
                     /translation="MKNDGAFVSSECRALPALILCMTCMLVALTAVSCGRSKPVAAAG
                     AVASSSSADTATEDAAAAEAAEAAAIAAAEAAKAAALAGTAIVAVVTPQRG"
BASE COUNT          268 a          414 c          474 g          288 t
ORIGIN      
        1 aagtagggga acagcggctc gtagcgtatg ccgaccagct cggagccctt tttgcgccag
       61 agtatctcgg gctgctggcc ttccttgtaa tacgcgccga ggcggctctc ggcgagcagg
      121 tagcgctcgg cgccgtcccg gacgagcacg tagtcgatgt ccgggccgag gcagagcgcc
      181 aggttcgacg gcagggtcca tggagtcgtc gtccacgcga ggaggtaggt agagccgtcg
      241 gcgagccctt cgagcccggc cggcgcctcg aggaccttga acttgatcgt gatggcgggg
      301 tcgtgcacgt ccttgtaacc gccgagcgcc agctcgtggt tcgacagcac cgtcgagcaa
      361 cgggggcagt acggtaggat atagtgaccc tcgtagacga ggccctggtc ccagagattc
      421 ttgacgaccc accagatcga ttccatatag tcgggatcca tcgtcttata atcgttgtcg
      481 aagtcgaccc atcgtccggc gcgggtcatg atggtgcgcc attccttgac gtagcgctgt
      541 accgaggctc ggcaggcttc attgaagttg gccacgccga agcgctcgat gtcggtcttg
      601 gagtcgaggc cgagctcctt ctcaatgagg ttctcgaccg gcagaccgtg gcagtcccag
      661 ccgaagcggc gctcgacctt gaagcctttc atcgtcttgt agcgggggat gacatccttg
      721 atcgtgcccg gaacgaaatg gccgaagtgg gggaggccgg tggcgaaggg cgggccgtca
      781 tagaatacgt attcgggaga gccatcccgc tcggagatgg accgcttgaa gacgtcgcga
      841 tcgttccaga acgcgaggac ggactcctcc atcgacggga aactggcttt cggatctaca
      901 ggctcgtaca ttgatgctcc tccgggacga tgtgactgcc gattatgcca taaagcggcg
      961 cgtcagttcc agcggtacga caccagtcgc gccctagtca aactctatcg atatgaatat
     1021 agtcatttca gcgattgcgt gaaaatagta ttgccaagtg ttatatttct atatgcatcg
     1081 tcgcgtcgcg gagccgcctt gacctatcct tgtacggtca aggcggtact ttttagcggc
     1141 ggcggtatcg tggagagcgt atgaaaaacg atggagcctt cgtatcgtcg gaatgccgag
     1201 cgcttccggc cctcatccta tgcatgacgt gtatgctcgt cgcgctgacc gccgtatcct
     1261 gcggtcgatc gaagccggtg gcggccgctg gggccgtggc ttcatcatcc tcggccgata
     1321 cggccaccga ggatgccgcg gcggcggaag cggcggaggc cgcggcgata gcggccgcgg
     1381 aggcggcaaa ggccgcggcc ttggccggta ctgccatagt agccgtagtg accccgcagc
     1441 gcgg
//
LOCUS       DAOOLN010000610         1443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_117315_length_1443_cov_3.491354, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000610 DAOOLN010000000
VERSION     DAOOLN010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117315_length_1443_cov_3.491354"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..948
                     /gene="amrB"
                     /locus_tag="PLW80_08085"
     CDS             118..948
                     /gene="amrB"
                     /locus_tag="PLW80_08085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPB66506.1"
                     /translation="MAIRARCLPDGWYPLSGAEAGAKLDAWARLDEQTRIGGAVAAIA
                     PHAGWYYSGRIAWSAWMSAGEADVVVIIGGHLPAGADFRMYREDGFDTPFGAAPADAE
                     LREVLARAVQVQGERGTDNTVEVHLPMAAHRFPGTPLVCLRAPNDERAARLGSELADY
                     QVSSGRRLFVLGSTDLTHYGKAYGFEPGGPAPGGFAWAGRANKDITDAFLAMDEAEAI
                     RQAELCGSACSVGAAVAAMSFARRLGASVSRLVMRGSSDELAPGSDSSVGYCAVTYSR
                     "
     gene            complement(976..>1443)
                     /locus_tag="PLW80_08090"
     CDS             complement(976..>1443)
                     /locus_tag="PLW80_08090"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HPB66507.1"
                     /translation="PTRIAAAAATFPGVEHRLEPIRDRRGVRWFNDSAATVPEAAKAA
                     VEAFDAPVVLITGGTDKKLEFETARSAYARAASVVLLSGTGTDKILPLLKADGVPYSG
                     PYSDIGLAVAEADRLALPGSVVVLSPGCTSFGMFKNEFDRGRRFKAAVAELPD"
BASE COUNT          242 a          460 c          481 g          260 t
ORIGIN      
        1 caggcgacga gagcgcctag aacgacgaac gagcatatgg ctataagtaa tggttcgcgt
       61 ttcatagtga tgcagtataa tcctaatatc ccttttttgg aaccgagagg aggccccatg
      121 gctatacgcg cccgttgtct gccggacgga tggtatcctt tatcgggggc cgaggccgga
      181 gcaaagctcg acgcctgggc taggctggat gagcagactc gaatcggcgg cgcggtagcg
      241 gccatcgcgc cccacgccgg atggtattat tctggcagga tagcctggtc ggcctggatg
      301 tcggcaggtg aagccgatgt cgtcgtcatc attggcgggc acctgccggc gggcgccgat
      361 ttcaggatgt accgggaaga cggcttcgac acaccgttcg gcgctgcccc cgcggacgct
      421 gagcttcgcg aggttctggc ccgtgccgta caggtccaag gcgaacgagg caccgacaac
      481 accgtcgagg tgcacctgcc tatggctgcc catcgattcc ccgggactcc gctcgtatgc
      541 ctgcgcgccc cgaatgacga gagggccgct cggcttggct cggagctcgc cgactaccag
      601 gtctcgtctg gccgccgcct cttcgtgctc ggatccaccg accttacgca ttacggcaag
      661 gcctacggct tcgagccggg aggcccggct ccaggtggct tcgcctgggc cgggcgcgcc
      721 aataaggata ttaccgacgc cttcctggcc atggacgaag ccgaggctat cagacaggcg
      781 gagttgtgcg gctccgcctg ctcggttggg gcagccgtgg ccgccatgtc attcgccagg
      841 cgactcgggg ccagcgtctc ccgcctagtc atgcgcggct cgagcgatga gctggcgccc
      901 ggtagcgatt cgtccgtcgg ttattgcgcc gtgacgtact cccgctagct ggcgcctgcc
      961 agggcttacg gcccgtcagt ccggtagctc ggccacggcg gctttgaagc gcctgccgcg
     1021 gtcgaattcg ttcttgaaca tgccgaacga agtgcatccg ggcgatagca ccacgacaga
     1081 accgggaagc gccaggcgat ccgcctcggc gacggccagt ccgatatcgg aataggggcc
     1141 gctgtacggc acaccatcgg ccttgagtag cggtagtatc ttgtcggtac ccgttcccga
     1201 aagaagcacg accgaggcgg ccctggcgta cgcggagcgg gccgtctcga actcgagctt
     1261 cttgtcggtg ccgcctgtta tgagcacgac gggagcgtcg aacgcctcta cggccgcctt
     1321 cgcggcctcg ggcacggtgg ccgccgaatc gttgaaccag cggacgccgc gcctatcgcg
     1381 tatgggctca agccgatgtt cgacgccagg gaacgtagcc gcggccgcgg cgatcctggt
     1441 cgg
//
LOCUS       DAOOLN010000611         1442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_117481_length_1442_cov_2.431867, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000611 DAOOLN010000000
VERSION     DAOOLN010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117481_length_1442_cov_2.431867"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..769)
                     /locus_tag="PLW80_08095"
     CDS             complement(107..769)
                     /locus_tag="PLW80_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HPB66508.1"
                     /translation="MIEYVENKEIIARRIARFFASGDVVNLGIGLPTMVGNFVPEGVT
                     VILQSENGMMGLGPAPEKGKEDPMLVNAGGMPVSILPGGCFFDSATSFGIIRGGHVDY
                     TVLGVLEVDQEGNLANYKIPGKMVPGMGGAMDLVAGSKKVIAATTHLEKSGSSKLKHR
                     CSLPLTAAHEVDIVVTDVGYFTVKDGKFRLEEYFAPYTPEWIVEHTGADVVVAPGCKE
                     FK"
     gene            complement(782..>1442)
                     /locus_tag="PLW80_08100"
     CDS             complement(782..>1442)
                     /locus_tag="PLW80_08100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HPB66509.1"
                     /translation="SDRALIGKTFGANEELDGALAGRPMWKSGPVSKEEHAAGAKDLT
                     LVCNDTGIYNEAKGVSNGPAPLVKAKAFKTMIVSHIGTNPETQRQMNAGESDVRLVPQ
                     GTLAERIRAAGNGLGGVLTPTGVGTEVEEGKRKLTLKGKDYLLEEALSGDVAIIKAKK
                     ADKAGNLVYAKTARNFNPLMATACGTVIVEAEEIVEVGAIDPDQVHTPSIFVDFIVQA
                     E"
BASE COUNT          210 a          492 c          448 g          292 t
ORIGIN      
        1 cctaccgccg tcctcgcggc gcctacgatg aatgcgtctt tcattgattc cccccccagg
       61 aacatgaaat gaaaaggccg cccgtcgcgc gggcggcccg tcatgcttac ttgaattcct
      121 tgcagcccgg ggccacgacg acgtcggcgc cggtatgctc gactatccac tccggcgtgt
      181 acggagcgaa gtattcctcc agacggaact tcccgtcctt gaccgtgaag taaccgacgt
      241 cggtcacgac gatgtccacc tcgtgggcgg ccgtcaaggg cagggagcag cgatgcttaa
      301 gcttcgacga gcccgacttc tcgaggtggg tcgtcgccgc tatgaccttc ttggagcccg
      361 cgacgagatc catggcgcct cccatgccgg ggaccatctt gccgggtatc ttgtagttgg
      421 ccaggttgcc ctcctggtcg acttcgagca cgcccagtac ggtatagtcg acgtgcccgc
      481 cgcggatgat gccgaagctc gtggccgagt cgaagaagca gccgccgggc aggatgctca
      541 ccggcatgcc gccggcgttg acgagcatcg ggtcttcctt tcccttctcg ggcgcgggcc
      601 ctaggcccat catgccgttc tccgactgca ggatcacggt cacgccctcg ggcacgaagt
      661 tgccgaccat cgtcggtagg ccgatgccga ggttgacgac gtcccccgac gcgaagaagc
      721 gcgcaatcct gcgcgcgatg atttccttgt tctctacgta ctcgatcatg gcggcgctcc
      781 cttactcggc ctggacgatg aagtcgacga agatgctcgg cgtgtgcacc tggtccgggt
      841 ctatcgcgcc gacctcgacg atctcctcgg cctcgacgat gaccgtcccg caggccgtgg
      901 ccatcagcgg gttgaagttg cgggccgtct tggcgtagac gagatttccg gccttgtcgg
      961 ccttcttggc cttgatgatg gccacgtcgc cggagagcgc ctcctcgagc aggtagtcct
     1021 tgcccttaag ggtcagcttc cgcttgccct cctccacctc ggtgccgacg ccggtcggcg
     1081 tcagcacgcc gcccagtccg ttcccggccg cgcggatacg ctccgccagc gtgccctgcg
     1141 gaacgagccg gacgtccgat tcgcccgcgt tcatctgccg ctgggtctcc gggttggtgc
     1201 cgatatgcga gacgatcatc gtcttgaagg ccttggcctt taccagcggc gccggtccgt
     1261 tcgatacgcc cttggcctcg ttatagatgc cggtatcgtt gcagacgagg gtcaggtcct
     1321 tcgcgcccgc ggcgtgttcc tccttgctca cgggcccgct cttccacatg ggccggccgg
     1381 cgagggcgcc gtcgagctcc tcgttggcgc cgaaggtctt gccgatgagg gcccggtcgc
     1441 tc
//
LOCUS       DAOOLN010000612         1440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_117717_length_1440_cov_2.918412, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000612 DAOOLN010000000
VERSION     DAOOLN010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117717_length_1440_cov_2.918412"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PLW80_08105"
     CDS             <1..746
                     /locus_tag="PLW80_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HPB66510.1"
                     /translation="GANARPNVNWLKMVKTSKARSKIRAWLVAEGTVLAIDKNVVARK
                     RDDRHDDKGKPHAGERRPELEVRHGEARHTEPKKPEAPVAAMSLMDYRPYIPGEATDS
                     VKAGISVSGAGDLMVRFAACCKPAVGERIVGYVSRGRGIIVHRASCKNLPAIREFAER
                     RVEVSWESAPSLTRRYHVTGRRVLDLFSEIENAVRKHGGRLVEGRLEDGQETLSGFFT
                     MAYPSAEDARTAERNLKHVPSILKIKRVD"
     gene            910..>1440
                     /locus_tag="PLW80_08110"
     CDS             910..>1440
                     /locus_tag="PLW80_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66511.1"
                     /translation="MNNGFVRWFLVKHAGAEPMERQQIGALLYTLAAIGVGAIAVAVV
                     MANPVIRLALIVLVLLSAALMLLVRAGFAVFSSVAATTLLSSMFAAIPFLQPYRHEYE
                     LYLLATLECFALIITGLIARRRWQSLVVLASALAALVSDFALRVIPNAGEGGARYSDI
                     VITVLVVVIATAIERAI"
BASE COUNT          246 a          462 c          490 g          242 t
ORIGIN      
        1 cgggcgccaa cgccaggccc aacgtcaact ggctcaagat ggtcaagacg agcaaggccc
       61 ggtccaagat acgggcctgg ctggtggccg aggggacggt gctggccata gacaagaacg
      121 tcgtggcccg caagcgggac gacaggcacg acgacaaggg caagccgcac gcgggcgagc
      181 gccggccgga gctcgaggtg aggcacggcg aggccaggca tacagagccc aagaagcccg
      241 aggctccggt cgcggccatg tcgctgatgg actaccgtcc ctacatcccc ggcgaggcga
      301 cggattcggt aaaggccggc atcagcgtct ccggagccgg cgacctgatg gtccggttcg
      361 cggcctgctg caagccggcg gtgggcgagc gcatcgtcgg ctacgtctcg cgggggcggg
      421 gcatcatcgt gcaccgcgcg tcgtgcaaga acctgcccgc gattcgcgag ttcgcggagc
      481 gccgcgtgga ggtttcctgg gaatctgcgc catcgctgac gcggcgctac cacgtcacgg
      541 gcaggcgcgt cctcgacctg ttctccgaga tagagaacgc ggtccgaaag cacggcggcc
      601 gcctggtcga gggccgcctc gaggacgggc aggagacgct gtcgggattc ttcacgatgg
      661 cgtatcccag cgccgaggac gcgcggacgg cggagcgcaa cctgaagcac gttccgtcga
      721 tccttaagat caagcgcgtc gactagccgc ccggccccgg atcgggcgcg gatgtattga
      781 tattaccaga cgcttgaatt tgctgatttg aatatatata ctccgcgcta ttatgcttac
      841 agggcgcggc cggaccgcgc atgcggttat gtcacctccg cggtcagcgg cgatggggaa
      901 gggaattaca tgaacaacgg attcgtcaga tggttcctgg tcaagcacgc cggcgcggag
      961 ccgatggagc ggcaacagat cggggcgctg ctgtatacgc tggcggcgat cggggtggga
     1021 gctatcgccg tcgccgtagt catggccaac ccggtcatcc ggctggcctt gatcgtcctc
     1081 gtactgctgt ccgccgcgct catgctcctg gtgcgcgcgg gcttcgccgt cttctcgagc
     1141 gtagcggcca cgacgctcct ctcgtcgatg ttcgccgcca tcccgttcct gcagccctac
     1201 aggcacgagt acgagctgta cctgctggcg acgctggaat gcttcgcgct catcatcacc
     1261 ggcctgatcg ccaggcggcg ctggcaatcg ctggtggtcc tggccagcgc cctcgccgcg
     1321 cttgtatcgg acttcgcgct ccgcgtgatc ccgaacgcgg gcgaaggcgg cgccagatat
     1381 agcgacattg tcatcaccgt cctcgtggtt gtcatcgcta ccgcgatcga gcgcgccatc
//
LOCUS       DAOOLN010000613         1436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_118230_length_1436_cov_3.700941, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000613 DAOOLN010000000
VERSION     DAOOLN010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118230_length_1436_cov_3.700941"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /locus_tag="PLW80_08115"
     CDS             <1..1296
                     /locus_tag="PLW80_08115"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HPB66512.1"
                     /translation="RAASSPGQWITTNLFPPPLSNAIDMEALTEGMDFASWDNYPVWG
                     DQDEPLPWQFNATAQAYVRGLRDNVPFTVMEEMSGFQGHVCLGHLPPERQVALWAVQA
                     VARGANRVVFFRWRTAPFGQEQLCHGLVDGDDRETERLRVIAATMRSAKAELSSLASV
                     PVESPVCLAYSKDDARVLREQYLSKGLLLRVSPTFQAGYDKELASWYAPFPTLGVGAD
                     AAPIGSLEPERYRVISLPLYQMADSAVVDRLGHWVESGGSLVLGYRAGARDALDHNVE
                     ATLPGLFAELAGIRVPRFESLNETSAALRVGVVPGRGGVWADIIEPDGAETVAVWTDR
                     SKFYRGSPAVTVNRVGKGLVWYLGTSPNPIAMLALYRRILKAAGLCPRFAGADVEAIE
                     RRDTDGKTWRLVLNHSPRRRRAYGATLEPWGWVKLPVSE"
BASE COUNT          251 a          480 c          457 g          248 t
ORIGIN      
        1 cgcgcagcct cgtctccagg ccagtggata acgaccaacc tgttcccgcc tcccctgtcc
       61 aacgctatcg acatggaagc gttgacggaa ggcatggact tcgcctcgtg ggataactac
      121 ccggtctggg gcgaccagga tgagccgcta ccgtggcagt tcaacgcgac cgcccaggcc
      181 tacgtccgag gactacggga caacgtcccg ttcacggtga tggaggagat gtcaggcttc
      241 cagggccacg tctgcctcgg tcacctgcct ccggaacgcc aggtcgctct ttgggccgtc
      301 caggccgtcg cccgcggcgc gaaccgcgtc gtattcttcc gctggcgcac cgctcccttc
      361 ggacaggagc agctgtgcca cggcctcgtc gacggcgacg accgggagac ggagcggcta
      421 cgggtgatcg ccgcgaccat gcgcagcgcc aaggctgagc tgtcgtctct ggcttcggtc
      481 cccgtcgaga gtcccgtctg cctagcgtat tccaaggacg acgcgagagt cctgcgggag
      541 caatacctgt ccaaggggct actccttcgc gtctctccga cgttccaggc tggctacgac
      601 aaggagctcg cctcgtggta cgcgccgttc ccgacgctcg gagtcggcgc cgacgcggcc
      661 ccgataggat cgctcgagcc cgagcgctac cgcgtcatat cgctaccgct ctaccagatg
      721 gccgactccg cagtggtcga ccggctcggc cattgggtcg aaagcggcgg tagcctggtc
      781 ctcggctatc gagccggcgc ccgggacgcc ctcgaccata acgtcgaggc gaccctaccg
      841 ggcctgttcg ccgagctggc gggcatcagg gtgccacgct tcgagtcgct caacgagacg
      901 agcgccgccc tgcgcgtcgg cgtcgtgcca ggccgcggcg gcgtctgggc cgacatcatc
      961 gagccggacg gcgccgagac ggtggccgtc tggaccgacc gttcaaagtt ctatcgtggc
     1021 agcccggccg tcaccgtcaa ccgggtcggc aagggcctcg tctggtacct cggcaccagc
     1081 ccgaacccga tagcgatgct ggccctgtac cggcgtatcc tcaaggccgc cggcctgtgc
     1141 ccgcgtttcg ccggcgcaga cgtagaggct atcgagcgcc gcgacaccga cggtaagacg
     1201 tggcgactgg tcttgaacca ttcgccgagg cgcagacgag cctacggggc gacgctcgaa
     1261 ccctggggct gggtcaagtt gcccgtcagc gaatagacgg aataaaacgg atttgccgag
     1321 gcgacgaaga ggaaaaagga tacgagatac gaaaggggaa gtctgaagaa agagctttaa
     1381 ggcttttggt cctttcaaag cttttctctt tctctcctct aatactcgta tcctct
//
LOCUS       DAOOLN010000614         1435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_118389_length_1435_cov_2.719565, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000614 DAOOLN010000000
VERSION     DAOOLN010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118389_length_1435_cov_2.719565"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PLW80_08120"
     CDS             complement(<1..265)
                     /locus_tag="PLW80_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66513.1"
                     /translation="MKRITHTAFAIAVVATLAVATYFFLSKRDGRKADEPVRYRALEA
                     VDATNPSERPIEAAQAIRAAASIPLEPNETLLDLYSFNLDYDDE"
     gene            complement(304..1299)
                     /locus_tag="PLW80_08125"
     CDS             complement(304..1299)
                     /locus_tag="PLW80_08125"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPB66514.1"
                     /translation="MTIDEAAAFLSGRDDFILSSHEGPDADGLGAEYALARALSSLGK
                     RVRVVNANRHSETYRFIDRAGIIECIDDAGLDDEEIRASTCVLLDTNDIMYAGEVADR
                     IIAKAKGLIIIDHHEVKGDAAPNVCSAPSYSSTCEMLYLILKRLGHVFTEETASALFA
                     GMVYDTGSFAYSKTTADTFEAALELVRAGADPTKIHGALYESSAVNVLLLRKDVLATL
                     ELYGGNKIAVQTLTSGMLAETGSSSQDAEGLINVPLQAADVEVSIFLKENEEGLLRCS
                     LRSKGAINVAQIAQSFGGGGHRSAAGFKSPYPLDTIKGRVLELVVIALAQRQDSD"
BASE COUNT          230 a          437 c          482 g          286 t
ORIGIN      
        1 cctcatcgtc gtagtcgagg ttgaacgagt acaggtcaag cagggtttcg ttcggctcga
       61 gcggtataga cgcggcggcc cggatcgcct gggccgcctc gatcgggcgc tccgatggat
      121 tggtggcgtc tacggcttcg agggcccgat agcggaccgg ttcgtcggcc ttgcgtccgt
      181 ctcgcttgga gaggaagaaa taggttgcga ccgcgagcgt cgcgacgacg gctatggcga
      241 atgctgtatg ggttatacgt ttcatgcggc gtatcgttcc ttgtttgcgg ccgtctctga
      301 accctagtcg gaatcctgcc gctgagccag cgctatgacg acgagttcta gcactcggcc
      361 cttgatcgta tcaaggggat agggcgactt gaagccggcg gcgctccgat gaccgcctcc
      421 gccgaaggac tgggctatct gggctacgtt gatcgcccct ttggacctga gagagcacct
      481 gagcagaccc tcctcgttct ccttgaggaa gatcgagacc tcgacgtcgg ccgcctggag
      541 cggtacgttg atgaggccct cggcgtcttg gctcgatgaa cccgtctcgg cgagcatgcc
      601 ggaggtaagc gtctgtacgg cgatcttgtt gccgccgtat agctcgaggg tggcgagcac
      661 gtccttgcgt agcaggagca cgttcacggc cgacgactcg tagagcgcgc cgtggatctt
      721 cgtcggatcg gctccggcgc ggaccagctc cagcgcggcc tcgaaggtgt cggccgtcgt
      781 cttggagtag gcgaaggagc cggtgtcgta gaccatgccg gcgaacaggg ccgaggccgt
      841 ttcctcggtg aacacgtgcc cgagtcgctt tagtatcagg tagagcatct cgcaggtcga
      901 cgagtagctc ggcgcggagc agacgttcgg ggccgcgtcg ccctttacct cgtggtggtc
      961 gatgattatc aatcccttgg ccttggcgat gatgcgatcg gccacctcgc ccgcgtacat
     1021 tatgtcgttc gtgtcgagta gcacgcaggt cgaggctcgt atctcctcgt cgtcgagccc
     1081 ggcgtcgtcg atgcactcga tgatgccggc cctgtctatg aacctgtagg tttcggagtg
     1141 cctgttggcg ttgacgacgc gcacgcgttt gccgagcgac gaaagggctc tggcgagggc
     1201 gtactcggct cccagcccgt ccgcgtcggg gccctcgtgc gacgaaagga taaaatcgtc
     1261 ccggccggac aggaacgccg cggcctcgtc gatcgtcatg cggtaggttc ctccggctcg
     1321 ttcccagcgc ccctatccct gcgacgccga cgcctaaacg cgacgatggc cacgaccaag
     1381 cctatggctc cagccgccgc caggctcgcg gccaagggcg cgccggcgaa ggagg
//
LOCUS       DAOOLN010000615         1434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_118466_length_1434_cov_4.054387, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000615 DAOOLN010000000
VERSION     DAOOLN010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118466_length_1434_cov_4.054387"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1434
                     /locus_tag="PLW80_08130"
     CDS             <1..>1434
                     /locus_tag="PLW80_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66515.1"
                     /translation="ALRLYPLARDEAAALASAYWPQIPERLLEAVLAQAGGNPLFIRE
                     YALFARKHRDLSSLPGTIQNMFIASLERFEPAARELATACSVFLLNFDTEEAARVYEA
                     AGGDPTTVPASLEALCADGVLVQSGERFAFSLDVLRKALYSTILNHNKRILHGVIADI
                     MLSKPATNRFRLIRHLMKAERWLEAADAIRSDPERNYGYEYLAIIDPLYKRLAKPHPD
                     AAVQLLITKSALNFNAGKIDEAEEDLKRVMRIAVNERNRLCMGFAYHMICAHNVMASS
                     FQKARFTGQKALHYYRQAGLAARSVQNVVRHVALSEIQRGNFEEARQMVASTAAIPGR
                     DEFEYASAVAEERLYSGDYRGALAAVESVGDRSDGSYGAVARFFGNDLRLKVLWQLCD
                     FRALGPAAKALLDSGGLSDAIVSQAHAMLAASATLYGDAKAARESFMQAEYYADKVRN
                     DYDRIESSRTLALCHFVAGNARKAETAA"
BASE COUNT          237 a          526 c          460 g          211 t
ORIGIN      
        1 gcgctgcgcc tctacccgct cgcccgcgac gaggcggccg cgctcgcctc ggcctactgg
       61 ccgcagatac ccgagcgcct gctcgaggca gtgctggccc aggccggggg caacccgctg
      121 ttcatcaggg agtacgcgct gttcgcccgc aagcaccgcg acctgtccag cctgcccggc
      181 acgatccaga acatgttcat agcgagcctc gagcgcttcg agccggccgc gcgcgagctg
      241 gccacggcct gttcggtgtt cctgctgaac ttcgacaccg aggaggccgc gcgcgtctat
      301 gaggcggccg gcggcgatcc gaccacggtg cccgcctcgc tcgaggccct gtgcgccgac
      361 ggcgttctcg tccagtcggg cgagcgcttc gccttctcgc tcgacgtgct cagaaaggcc
      421 ctgtactcga ccatactcaa ccataacaag cgcatcctgc acggcgtcat cgccgacatc
      481 atgctgtcca agccggccac gaaccgcttc aggctgatcc gccacctgat gaaggccgag
      541 cgctggctcg aggcggcgga cgctatacgc tccgaccccg agcgcaacta cggctacgag
      601 tacctggcca tcatcgaccc gctgtacaag cgcctggcca agccccatcc cgatgcggcc
      661 gtgcagctcc tgatcaccaa gagcgcgctg aacttcaacg ccggcaagat cgacgaggcc
      721 gaggaggacc tgaagcgcgt gatgcggatc gcggtcaacg agcgcaaccg gctatgcatg
      781 ggcttcgcct accacatgat ctgcgcccat aacgtcatgg cctcgtcgtt ccagaaggct
      841 cgcttcaccg gccagaaggc cctgcactac tatcgccagg ccggcctagc cgccaggagc
      901 gtgcagaacg tcgtgcgcca cgtcgcgctc tcggagatcc agcggggtaa cttcgaggag
      961 gcccggcaga tggtcgcgag caccgcggcg atccccggcc gggacgagtt cgagtacgcg
     1021 agcgccgtgg ccgaggaacg gctctattcc ggcgactacc gcggcgcgct cgccgccgtg
     1081 gagtcggtcg gcgaccggtc cgacgggagc tacggcgcgg tggcccgctt cttcggcaac
     1141 gacctcaggc tcaaggtgct ctggcagctc tgcgacttcc gcgccctcgg gcccgccgcg
     1201 aaggcgctgc tcgactcggg cggcctgagc gacgcgatcg tctcgcaggc ccacgcgatg
     1261 ctcgcggcct cggccaccct gtacggcgac gccaaggccg cgcgcgagtc gttcatgcag
     1321 gccgagtact acgccgacaa ggtgaggaac gactacgacc gcatcgagtc gtcgaggacg
     1381 ctcgccctgt gccacttcgt ggccggcaac gctcgcaagg ccgagaccgc cgcc
//
LOCUS       DAOOLN010000616         1433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_118636_length_1433_cov_2.819303, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000616 DAOOLN010000000
VERSION     DAOOLN010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118636_length_1433_cov_2.819303"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PLW80_08135"
     CDS             <1..918
                     /locus_tag="PLW80_08135"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPB66516.1"
                     /translation="FSEARAIMEMCRIAGVPDSTAALWSIRELDACIKAFAKKYRAEG
                     KERDRGAIVFMEKLYEFRKRLEFELPKYRAGITSSRFIRANQRIRVLVEGIGVYSSTV
                     IDSNERYLVVSYPVGARVPPGFQWKGARVSVYFWRQEDAGYVFDTYVLEDLRIRDIPV
                     LQLGHSESLLRTQKRKSLRVRSRMPAYLYLLKRLEGAYEKPERVPGLKCVVQDVSEDG
                     VSVLIGGKARIGLLVKAQFYIGDDQIVISGTVKGSEYDVEKNQSLLHVEAMTPSPRMR
                     NVIRSHVYNVGPGDSERRGAELDERPFSV"
     gene            939..>1433
                     /locus_tag="PLW80_08140"
     CDS             939..>1433
                     /locus_tag="PLW80_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66517.1"
                     /translation="MHRIRVAIAFMLAVALTGASAQESSELIDTYRRNFVRSSLGTKL
                     ELLKEASAYDGVDMGPLYDTAIQFVLSNASLLSTDALLRDISVLSVNMVRKYKYAPAA
                     GHLWELFGIYKDALVRVPLLQTLAEVAVGNPAILKELNAFVDTQVSLAKSGVRIDQAV
                     LDAAI"
BASE COUNT          264 a          470 c          450 g          249 t
ORIGIN      
        1 ttctccgagg ccagggccat catggagatg tgcaggatag ccggcgtgcc cgattcgacg
       61 gcggccctct ggtcgatacg cgagctcgac gcctgcatca aggccttcgc caagaagtac
      121 cgcgccgaag gcaaggagcg cgaccgcggc gccatcgtct tcatggagaa gctgtacgag
      181 ttcaggaagc ggctcgagtt cgagctgccc aagtaccgcg ccggcatcac cagttcccgc
      241 ttcatacggg cgaaccagcg catccgcgtg ctcgtcgagg gtatcggcgt ctactcgtcc
      301 acggtgatcg actcgaacga acgctacctg gtcgtctcct acccggtcgg cgccagggtg
      361 ccgcccggct tccagtggaa gggcgcccgc gtgtcggtgt acttctggcg ccaggaggac
      421 gccggctacg tgttcgatac ctacgtgctc gaggatctgc gcatccgcga cataccggtg
      481 ctgcagctcg ggcactcgga gtcactccta cgcacccaga aacgcaagtc gctgcgcgtc
      541 cgctccagga tgccggccta cctgtacctg ctcaagcgcc tcgagggcgc ctacgagaag
      601 ccggagcgag tgcccggcct caagtgcgtg gtgcaggacg tgtcggagga cggcgtatcg
      661 gtgctgatag gcggcaaggc caggatcggg ttgctcgtca aggcgcagtt ctacataggc
      721 gacgaccaga tcgtcatcag cggcaccgtc aaaggctcgg agtacgacgt cgagaagaat
      781 caatccctat tgcacgtcga ggcgatgacg ccgagcccgc gtatgcgcaa cgtcatccga
      841 tcccacgtct acaacgtcgg tccaggcgat tcggagcgcc ggggcgccga actggacgag
      901 aggccgtttt cggtataagt ttaacggacg ggggaacgat gcataggatc cgcgtagcga
      961 tagcgttcat gctggccgtc gcgctcaccg gcgcgtcggc ccaggaatcg tccgagctca
     1021 tagacaccta ccgtaggaat ttcgtgcgct cgagcctcgg caccaagctg gagctgctca
     1081 aggaggcctc ggcctacgac ggcgtcgaca tggggcccct gtatgacacg gccatccagt
     1141 tcgtgctcag caacgcctcg ctcctgtcca ccgatgcgct cctgcgcgac atatcggtgc
     1201 tatcggtcaa catggtgcgt aagtacaagt acgcgccggc cgccggccac ctctgggagc
     1261 tattcggtat ctacaaggac gccctcgtcc gcgtgccgct cctgcagacg ctggccgagg
     1321 tcgccgtagg caaccccgcc atcctcaagg agctcaacgc cttcgtcgac actcaggtct
     1381 cgctcgccaa gtcgggcgtc cggatcgacc aggccgtgct cgatgccgcc atc
//
LOCUS       DAOOLN010000617         1432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_118757_length_1432_cov_3.220044, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000617 DAOOLN010000000
VERSION     DAOOLN010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118757_length_1432_cov_3.220044"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="PLW80_08145"
     CDS             complement(<1..934)
                     /locus_tag="PLW80_08145"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HPB66518.1"
                     /translation="MKRYLVALVCAMTALAAHAQFVDLGESMLYVRVGFEDSWTRGLP
                     RLDADGWTVLEPSGGKRALIMRDLGLPGSPEGGVLSLFRREPIEYTVLAPFQADLTLL
                     NSSDPALFLKHVGQSWAVYLNGVLLRNEFVSYGEGYKERSLVDVVVPLDKRLLTRGSN
                     LLAFRIRGDPVDDRTGFNQPGPYVIDSYRSLVSRNGEYVDLMLIGVYAFFALYHGVLF
                     ALRPKDRGYLYFSASALLMALYMWSRSAIATELVLDTALLRYVEYASLFMLLPAVVAF
                     IRTVLDDRPFRMPAILAAASAVLALACLFLRLEAM"
     gene            1064..1372
                     /locus_tag="PLW80_08150"
     CDS             1064..1372
                     /locus_tag="PLW80_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66519.1"
                     /translation="MKGTIKTIATLAVIGAALYFTNPSMEDFGRYYERKLVAESQQGT
                     ADPLKKIAKALAQTGADLAIKAGAKRQDKLLFSVFTLGPASKPAHRYLGLAKFVFVEL
                     "
BASE COUNT          280 a          447 c          485 g          220 t
ORIGIN      
        1 gcatcgcctc gaggcgcagg aagaggcagg ctagcgccag gacggccgac gccgcggcga
       61 gtatcgccgg catcctgaag ggccggtcgt ccaggacggt ccggatgaac gccaccaccg
      121 ccggcagcaa catgaacagg gaggcgtact cgacgtaacg gagcaaggcc gtatccagta
      181 ccagctcggt cgctatcgcg ctcctcgacc acatgtagag cgccatcagc agggccgagg
      241 cgctgaagta caggtagccc ctgtccttag gcctgagcgc gaacaggacg ccgtggtaca
      301 acgcgaagaa ggcgtagacg cctatgagca tcaggtcgac gtactcgccg ttacgcgaga
      361 cgagcgaccg atacgagtcg atcacgtagg gccccggctg gttgaagccg gtcctgtcgt
      421 cgacagggtc gccgcggatc ctgaaggcga gcaggttgga gcctcgcgtc agcaggcgct
      481 tatccagggg cacgaccacg tctaccaggg atcgctcctt atagccttcg ccgtagctca
      541 cgaattcgtt gcgcagcagt acgccgttca ggtagacggc ccacgactgc ccgacgtgct
      601 tgaggaacag ggccgggtcg ctggagttga gcagggtgag atcggcctgg aagggcgcga
      661 gtaccgtgta ctcgatcggc tcgcggcgga agaggctgag gacgccgccc tcgggggagc
      721 cgggcaggcc gagatcgcgc atgatcaggg cgcgcttacc gccgctcggc tcaagcacgg
      781 tccagccgtc ggcgtcgagc ctgggcaggc ccctggtcca ggagtcctcg aacccgacgc
      841 gaacgtagag catggactcg cccaggtcga cgaattgggc gtgggccgcc agggccgtca
      901 tggcgcatac cagcgcgacg aggtatcgtt tcatagcgtt accacatcct acggccccat
      961 cccgcccggg tcaagccgga atacgcgcgg agtccggctt gcggaacgcc cggcgccggg
     1021 ctattgttca tggtctccgg cgtatattta gaaaggaaac ggcatgaaag gcaccatcaa
     1081 gaccatcgcg accctggccg tcatcggggc ggcgctctac ttcaccaatc cgtcgatgga
     1141 agacttcggc aggtactacg agaggaagct ggtggccgaa agccaacagg gtaccgccga
     1201 tccgcttaag aagattgcca aggcgctggc gcagaccggc gcggacctgg cgatcaaagc
     1261 cggcgccaag cggcaggaca agctgctgtt cagcgtcttc acgctcggcc cggcgtccaa
     1321 gccggcgcat cgctatctgg gcctggccaa gttcgtcttc gtggagctgt agggccctcg
     1381 gccatggggc tgttctcgtt cctgcgccgc aggaagccgg aggattacga cg
//
LOCUS       DAOOLN010000618         1431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_118986_length_1431_cov_2.113372, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000618 DAOOLN010000000
VERSION     DAOOLN010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118986_length_1431_cov_2.113372"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="PLW80_08155"
     CDS             complement(<1..947)
                     /locus_tag="PLW80_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66520.1"
                     /translation="MRIRLARLAAATALPLALTSCSFLQGLFGSDDGTEDGSGEASSS
                     AEAVSVAISEPTTVRTIKSSGSLTLTVSLESAGDVYFVFTNSSLGSYADEPGVEPSAS
                     SARSSAAGGTAVARTTPGSLGLSGPPPAGRPPRGAPEVTDRNAELRDELRGGKAEADP
                     YGAARSVSGSASFEVDEAALGSVGDVRDFYDYETVANAVPVTLRYVSGATATAMGERA
                     LRVWVADDCWDDDDDSLDGDKSLLVSQAMTDAYAGKFLSGEAGTDNDIYDWVTKVLGA
                     EWGDVGSSSLIGFDGVIDIVLLDIDDDGSTTGGVLGYFYG"
     gene            1149..>1431
                     /locus_tag="PLW80_08160"
     CDS             1149..>1431
                     /locus_tag="PLW80_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66521.1"
                     /translation="MAIKTSSGPGSELDRILRDVGQPDTRALFYFFSSELAGSGIVEA
                     VNGRFPGTACVGASMIGGWSIAPAFEKGVTAMTLSSEEVEESFLSMAEGV"
BASE COUNT          245 a          475 c          481 g          230 t
ORIGIN      
        1 ccgtagaaat acccgagcac tccgccggtc gtcgatccgt cgtcgtcgat atcgagcagg
       61 acgatatcga tgacgccgtc gaaaccgatg agcgaggagg agccgacgtc gccccactcc
      121 gcgccgagga ccttggtgac ccagtcgtaa atatcgttgt cggtaccggc ctcccccgac
      181 aggaatttcc cggcgtacgc gtccgtcatc gcctgcgaga cgaggaggga cttgtcgccg
      241 tcgagcgagt cgtcgtcgtc gtcccagcaa tcgtcggcga cccagacgcg aagcgcgcgc
      301 tcgcccatgg cggtggccgt cgcgccggag acgtagcgga gcgtcaccgg cacggcgttc
      361 gccacggtct cgtagtcgta gaaatcgcgg acgtcgccga ccgagccgag cgcggcctcg
      421 tcgacctcga acgacgcgct ccccgagacc gacctagcgg cgccgtacgg gtcggcctcg
      481 gccttcccgc cgcgcagctc gtccctgagc tcggcgttgc ggtcggtaac ctcgggcgcg
      541 cctcgcggcg gacgtcccgc gggcggcggc ccgctcaagc cgagcgatcc gggcgtcgtc
      601 cgggctaccg cggttccgcc ggcagcggag gaccgggccg acgaggccga cggctcgacg
      661 ccgggctcgt cggcgtagga gccgagcgac gaattggtga agacgaaata gacgtcgccg
      721 gccgactcga gggagaccgt aagggtcagg ctccccgacg acttaatcgt ccggacggtc
      781 gtcggctcgg atatcgcgac gctgacggcc tcggcggacg aagacgcctc gccgcttccg
      841 tcttcggtcc cgtcatccga gccgaaaagg ccctgcagga acgagcatga agtgagcgcg
      901 agcggtagcg ccgtcgcagc ggcgaggcgc gctagtcgta ttctcatggt tgtctccgcg
      961 atcgggcccc gggcccgtca ttatgtaaat atcccggcga tgaccgacga tacggcctcg
     1021 gctgtaacga tactgtaaac tccggacagt ctcgctccga tccgccgccg gcagcccggt
     1081 tcgtcagtag gtagcacgtt gacacggccc ttgatcgcgc ttaccctctc atggaaaaag
     1141 gagcgattat ggcgatcaag acctcctcgg gccccggatc ggagctagac aggatactcc
     1201 gggacgtcgg gcaaccggat acgcgcgcgc tgttctactt cttctcgagc gagctcgccg
     1261 gctccggtat agtcgaagcg gtcaacgggc gattcccggg aaccgcctgc gtcggcgcgt
     1321 cgatgatagg cggctggtcg atcgccccgg cgttcgagaa gggcgtcacg gccatgacgc
     1381 tgtcctcgga agaggtggaa gaatcattcc tatcgatggc cgagggcgtt a
//
LOCUS       DAOOLN010000619         1427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_119448_length_1427_cov_3.049563, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000619 DAOOLN010000000
VERSION     DAOOLN010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119448_length_1427_cov_3.049563"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="PLW80_08165"
     CDS             complement(<1..1224)
                     /locus_tag="PLW80_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66522.1"
                     /translation="MMFDLGNLVAIGITLIILLAYRILDRDNRSLEKVKKYAEKLREE
                     LSAHADQRAEDLKAYAIDLEVHQKAAKEVLRRVQLAEEALSAKADQIGGMAARIAEYD
                     KALAELKDMSSRVDENLSVIHDESAFVDGVARTLKAAKLEIEALKSSIPSIREGIAAD
                     AKDMMDDLRQAYSDELRSAMDDAQAQVEALRERAEQGTAEVAETNAEAVRAAEQRYRA
                     IDAMLADAFKRAREEGERLEDESFKRLEAQIDARGARLGEAIDTRFNALRDQAKEKVS
                     ETQGLLKSFKAEWRKDADELLAGARAEAEATVDKVGARMEDAEARAAKAEAKALETAQ
                     AVHAKVKDQLKAYQEDLAERQAAIRGAIKEGLVDTKAAAEGAVREMAESLSSFKSRID
                     ETAAEQAARVAAMDAS"
BASE COUNT          187 a          472 c          453 g          315 t
ORIGIN      
        1 cgacgcgtcc atcgcggcga cgcgggcggc ctgctccgcg gccgtctcgt cgatcctgga
       61 cttgaacgac gacagggact cggccatttc cctgacggcg ccctcggccg ccgccttggt
      121 gtctactaag ccttccttga tcgcgccgcg gatcgcggcc tgtcgctcgg ccaggtcttc
      181 ctggtaggcc ttgagctggt ccttgacctt ggcgtgtacc gcctgggccg tctccagggc
      241 cttggcctcg gccttggccg cgcgggcctc ggcgtcttcc atgcgggcgc cgaccttgtc
      301 gacggtcgcc tcggcctcgg cgcgggcgcc ggccaggagt tcgtcggcgt ccttgcgcca
      361 ttccgccttg aacgacttga ggaggccctg ggtctcggag accttctcct tggcctggtc
      421 gcgcagagcg ttgaagcggg tgtcgatggc ctcgccgagc ctggctccgc gggcgtcgat
      481 ctgggcctcg agccgcttga agctctcgtc ctcgagccgc tcgccttcct cccgcgcgcg
      541 cttgaacgca tcggccagca tcgcgtcgat ggcgcggtac cgctgctcgg ccgccctgac
      601 cgcctcggcg ttggtctcgg ccacctcggc ggtaccttgc tcggcgcgtt cccggagcgc
      661 ctcgacctgg gcctgggcgt cgtccatcgc cgagcgcagc tcgtcggagt aggcctggcg
      721 taggtcgtcc atcatgtcct tggcgtcggc cgcgatacct tcgcgtatac tgggtatcga
      781 cgacttcagg gcctcgatct cgagcttggc ggccttgagg gtacgggcga cgccgtcgac
      841 gaaggcgctc tcgtcgtgga tgaccgacag gttctcgtcg accctggagc tcatgtcctt
      901 gagctcggcg agcgccttgt cgtactcggc tatgcgcgcg gccatgccgc ctatctggtc
      961 ggccttggcg ctcagcgcct cctcggccag ctgcacccgg cgcagcactt ccttggcggc
     1021 cttctggtgc acctcgaggt ctatcgcgta ggccttgagg tcctcggcgc gctggtccgc
     1081 gtgggccgac agctcctcgc gcagcttctc ggcgtatttc ttgaccttct cgagcgagcg
     1141 gttatcgcgg tcgagaattc ggtaggcgag caggatgatc agcgtaatgc ctatggccac
     1201 gaggttgcca aggtcgaaca tcattatttc tcccattatc gattccgcct gggcgtgccg
     1261 gcggccggga tcggcccgat tggagtcagt atatagcact atggaggaat aataaagaat
     1321 cgagcttcgt cgacaggcgt gtcttgtaaa agctccattc tatgctatta tctgtacttg
     1381 tcaacatcag atctgacact tagctaaaaa tttaaagctg ttcactg
//
LOCUS       DAOOLN010000620         1423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_120004_length_1423_cov_3.490497, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000620 DAOOLN010000000
VERSION     DAOOLN010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120004_length_1423_cov_3.490497"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PLW80_08170"
     CDS             <1..648
                     /locus_tag="PLW80_08170"
                     /inference="COORDINATES: protein motif:HMM:NF039904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HPB66523.1"
                     /translation="YGQQDVDFVLQRLGLDESGRPGVTERGVMAVEALLFSKYQMYRA
                     VYWHKTVRAATAMIKKAVIVALDDGAVEPDELYGLDDGGFYSLMAGPGRDRSGLIASV
                     LHGAVLRPCLELAFDSGLVAHARAADLSLRPRLEEAIAGDAGAETVIVDVPEPISFES
                     DLPIIGTGSGFSDYATVFRPEVVASFTRSLRVIRVFSDGSPERVSAAALEALGGR"
     gene            645..>1423
                     /locus_tag="PLW80_08175"
     CDS             645..>1423
                     /locus_tag="PLW80_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HPB66524.1"
                     /translation="MSAAWEPSPELVDTAAGRWMGYVKRFMAAAGSGTHRYGLIRDGD
                     SVLVGLSGGKDSLALLLFLELRRRRVKDRYTLKAARVDWGAPPAAPEDEAALAAFCAW
                     LGVPFFEERRPMPESLAERGLSCYACSRERRRVLFELAEREGCGVVALGHHLDDFAET
                     ALMNVCKHGRLDPMRPQAVFFGRFRLVRPLCEARESALRAVATRLGLPVVRNRCPLAD
                     SSQRRAYKAALAELAKIDTLVRENVCKAASMAASPPEESHG"
BASE COUNT          193 a          473 c          524 g          233 t
ORIGIN      
        1 tacggccagc aggacgtcga cttcgtgcta cagcggctgg ggctcgacga gtcgggccgg
       61 cccggggtga ccgagcgcgg cgtgatggcc gtcgaggcgc tgctgttctc caagtaccag
      121 atgtaccggg cggtctactg gcataagacg gtgcgcgccg cgacggcgat gatcaagaag
      181 gcggtcatcg tcgcgctcga cgacggcgcg gtcgagccgg acgagctgta cgggctcgac
      241 gatggcggct tttactcgct gatggccggg ccggggcggg accggagcgg tctcatcgcg
      301 tcggtgctcc acggggcggt actcaggccc tgcctggaac tggccttcga ctctggcctg
      361 gtcgcgcacg cccgggccgc agacctgagc ctccggccgc gcctcgagga agccatagcc
      421 ggggacgccg gcgccgaaac ggtcatcgtc gacgtacccg agcccatatc cttcgagtcc
      481 gacctgccta taatcgggac cggctccggc ttctccgact acgcgacggt attcaggccc
      541 gaggtggtcg cgtccttcac gcgttcgctg cgggtgatac gcgtcttctc cgacggctct
      601 cccgagcgcg tctcggccgc cgccctggaa gcgctgggcg gccggtgagc gctgcctggg
      661 agccgtctcc ggaactcgtc gacacggcgg cggggcggtg gatgggttac gttaagcgct
      721 tcatggcggc cgccggatcc ggtacccatc gctacgggct gatacgcgac ggagactcgg
      781 tcctcgtcgg cctgtccggc ggcaaggact cgctggccct gctcctattc ctcgagctgc
      841 gcaggcggcg ggtcaaggac cgttatacgc tcaaggcggc ccgcgtcgac tggggtgcgc
      901 cgcccgccgc gccggaggat gaggcggcgc tcgcggcgtt ctgcgcctgg ctcggcgtgc
      961 cgttcttcga ggagcgccgg ccgatgcccg agtcgctggc cgaacggggc ttgtcgtgct
     1021 acgcctgctc gagggagcgg cggcgcgtcc tgttcgagct ggccgagcgc gagggctgcg
     1081 gcgtcgtggc gctcgggcat cacctggacg acttcgccga gacggcgctg atgaacgtgt
     1141 gcaagcacgg ccggctcgac ccgatgcggc cccaggccgt cttcttcggg cgcttcaggc
     1201 tcgttcgccc gctctgcgaa gcgcgcgagt cggccttgag ggcggtggcg accaggctcg
     1261 gcctgccggt cgtacggaac cgttgcccct tggccgactc gagccaacgc cgggcctaca
     1321 aggcggcgct tgcggaactc gctaaaatcg atacactggt acgggagaac gtatgcaagg
     1381 cggcgtcgat ggcggcgtcg cccccggagg aatcgcatgg aga
//
LOCUS       DAOOLN010000621         1423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_120052_length_1423_cov_2.421053, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000621 DAOOLN010000000
VERSION     DAOOLN010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120052_length_1423_cov_2.421053"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PLW80_08180"
     CDS             complement(<1..619)
                     /locus_tag="PLW80_08180"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPB66525.1"
                     /translation="MARLSAALAPVIALVLATLPTWALPAQEAASRSLNVIKVPTASG
                     GEERLYDESHALVVAVSRYTRGWPSLPGVRVDAERVEAALKAAGFSVTVVSDPDAEGL
                     RDAFEGFIQRYGQGVGNRLLFYFAGHGHTLRQSYGEEMGYIVPVDAPLPDRDPAGFLA
                     KAMDMQMIEVYAKRIQSKHALFLFDSCFSGSLFAITRAVPESIGYK"
     gene            complement(612..1121)
                     /locus_tag="PLW80_08185"
     CDS             complement(612..1121)
                     /locus_tag="PLW80_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66526.1"
                     /translation="MKARIVASCALAAILAGCVIYTFVDLGPWDFESATTVANPFSTS
                     VDNPWVVQNYGGRDSSPCLKSATLWTMGETSTLTLTYEGGLDDDKDLSFYLRINGSGS
                     IALRINGFLEESYDNSGYGYYSTITKVNVYDSSKYKSSGTNVLTWTFTCTDDYTDAYA
                     ILDEIEIDG"
     gene            complement(1124..>1423)
                     /locus_tag="PLW80_08190"
     CDS             complement(1124..>1423)
                     /locus_tag="PLW80_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66527.1"
                     /translation="VGLVSLVLAMAGRYYPTRTVPVWVGARLVPSFYLLANGNESIRL
                     VAYLDAGLSYALSDLGLDLRLSLFMTGSETMTDSGDMVGLVGLGLTLGYDALTGR"
BASE COUNT          265 a          434 c          471 g          253 t
ORIGIN      
        1 tcttgtagcc tatgctctcc gggacggccc gtgtgatggc gaagagcgag cccgagaagc
       61 atgaatcgaa caggaagagg gcgtgcttgc tctggatacg cttggcgtag acctctatca
      121 tctgcatgtc catggccttg gccaggaagc cggcggggtc acgatcgggt aacggcgcgt
      181 ccacggggac gatgtagccc atctcctcgc cgtaggattg cctgagcgtg tggccgtgac
      241 cggcgaagta gaagaggagc cggttgccga cgccctggcc gtagcgctgg atgaagcctt
      301 cgaaggcgtc gcgcaggcct tcggcgtccg ggtccgagac cacggttacg ctgaagcccg
      361 cggccttcaa ggccgcctcg acccgctcgg cgtcgaccct gacgccgggc aggctcggcc
      421 agcccctggt gtagcggctt accgcgacca ccagggcgtg gctctcgtcg tacagccgct
      481 cctcgccgcc ggaggccgtc ggtaccttga tcacgttgag gctccgggac gccgcctcct
      541 gggctggcag ggcccaggtc ggcagggtcg ccaggaccag cgcgatgacc ggcgccaagg
      601 ccgccgacag cctagccatc gatctctatc tcgtccagga tggcgtaggc atccgtataa
      661 tcatcggtac aggtaaaggt ccaggtcagg acgttggttc cggaagactt atatttcgac
      721 gaatcataga cgttcacttt cgtgatcgtc gaatagtacc cgtagccact gttatcgtag
      781 ctctcttcca ggaaaccgtt gatgcgaagc gcgatggaac cggaaccgtt tatcctcagg
      841 tagaacgaca gatccttatc atcgtcgagg ccgccctcgt aggtcagggt cagggtcgag
      901 gtctcgccca tcgtccatag cgtcgccgac ttgaggcagg gggaactgtc gcggccgccg
      961 tagttctgga cgacccaggg attatccaca ctggtcgaga aagggttggc cacggtcgtc
     1021 gcgctttcga agtcccaagg cccgaggtcg acgaaggtat agatgacgca tccggccagg
     1081 atcgcggcca gggcgcaact agcgactatt cgtgctttca tggtcatctc cccgtaaggg
     1141 cgtcgtagcc cagggtcagg ccaaggccga cgaggccgac catatcgccg ctatccgtca
     1201 tggtctcgct gccggtcatg aagaggctga ggcgcaggtc caggcccaga tcactgaggg
     1261 cgtaggagag gccggcgtcg aggtaggcga cgaggcggat gctttcgttg ccattggcca
     1321 ggaggtagaa cgacgggacg agcctcgccc ccacccagac gggaacggtc cgcgtcgggt
     1381 agtagcgccc ggccatggcc agcaccaggc tgaccaggcc gac
//
LOCUS       DAOOLN010000622         1423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_120053_length_1423_cov_2.418129, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000622 DAOOLN010000000
VERSION     DAOOLN010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120053_length_1423_cov_2.418129"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="PLW80_08195"
     CDS             <1..799
                     /locus_tag="PLW80_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HPB66528.1"
                     /translation="LAGSQEAFAGLMNAEAERLGLTETRFVEPSGLSELNTTTAREMA
                     VLARAYILRHPGALGDYHSRTTMHFPREDVMPAGAEPPAIKILLQTTNKLLFKYDGCD
                     GLKTGYVDESGYNLIATAERDGSRFIVVTLGGAGGPDSRTRSGAYLLDWAFANWKTVR
                     PEAPDPGSARAWGGASETVTLAYANTPVFTVQARLAGSIRCRIEATAEVEAPVSAGTR
                     LGQAVFTSGDVVVRRIDLVAAEDVALGNLFVHMRDALIRFFRRLFGG"
     gene            complement(818..>1423)
                     /locus_tag="PLW80_08200"
     CDS             complement(818..>1423)
                     /locus_tag="PLW80_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HPB66529.1"
                     /translation="ELAEVYEAWRGLGYNSRALRLRETARVCDERYGGVPPADERLLL
                     ELPGVGRYTARAVLAFAYDRPSAFLETNIRAALIFHFRPGRDRVGDRELEALAEAVLD
                     RDDPRTWYYALMDYGAWLKKREPNPTRRAAAYSTQSRFKGSVREARGAILRGLAGSGT
                     IALAEIAAEDGIEYARLERAACGLVRDGLIVRDGDALRFAD"
BASE COUNT          230 a          491 c          478 g          224 t
ORIGIN      
        1 gctcgcgggc tcgcaagagg ccttcgccgg ccttatgaac gccgaggccg aacggctcgg
       61 gctgaccgag acgcgtttcg tcgagccatc gggcctgtcg gaactcaata cgaccacggc
      121 ccgggagatg gctgtcctgg cccgcgccta catactgcgc caccctggcg ccctcggcga
      181 ctaccactcc aggacgacga tgcactttcc ccgcgaggac gtgatgccgg caggggccga
      241 gccaccggcc atcaagatac tcctccagac gaccaataag ctcctgttca agtacgacgg
      301 ctgcgacggc ctcaagaccg gctatgtcga cgagtcgggc tataacctga tcgccaccgc
      361 cgaacgcgac ggtagccgct tcatcgtcgt caccctcggg ggggcgggcg gccccgacag
      421 ccgtacgcgt tccggtgcct acttgctcga ctgggccttc gccaattgga agacggtgag
      481 gccggaggcg cccgatcccg gaagcgccag ggcctggggc ggggcttccg agacggtgac
      541 gctcgcgtac gccaatacgc cggtatttac cgttcaggcg aggctagccg gctctatacg
      601 ctgtcgtatc gaggccaccg ccgaggtgga agcgccggtg tcagccggca ccaggctcgg
      661 gcaggcggtc ttcacctccg gcgacgtcgt cgtcaggcgc atcgacctgg tggcggcgga
      721 ggacgtggcg ctcggcaacc tgttcgtgca catgcgcgac gcgctcatac gcttcttcag
      781 gcggctgttc ggcggctagg tcgcggcgag gcctcgatca gtcggcgaaa cggagcgcgt
      841 cgccatcgcg gacgatgagc ccgtcgcgga cgaggccgca ggcggcccgc tcgagccgag
      901 cgtactcgat gccgtcctcg gcggctattt cagccagcgc gatcgtcccg ctacctgcga
      961 gcccacgtag gatagccccc ctggcctccc tgaccgaacc tttgaaacgc gactgcgtcg
     1021 aataagcggc ggccctccgc gtcgggttgg gctcccgttt cttaagccag gcgccgtagt
     1081 ccatcagcgc gtagtaccag gtcctcggat cgtcacggtc gagcactgcc tcggccaggg
     1141 cctcgagctc tcgatcgccg actcggtctc tgccggggcg gaagtgaaag atgagggcgg
     1201 cccggatgtt cgtctcgagg aacgccgagg gtcgatcgta ggcgaaggcc aacacggccc
     1261 tggccgtgta gcggccgacg ccgggtagct cgaggagcaa gcgctcgtcg gccgggggaa
     1321 cgccgccgta tcgctcgtcg caaacccgcg cagtctccct gaggcgtagg gcccgcgagt
     1381 tgtagcccag cccacgccac gcctcgtaga cctcggcgag ctc
//
LOCUS       DAOOLN010000623         1419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_120524_length_1419_cov_3.747067, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000623 DAOOLN010000000
VERSION     DAOOLN010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120524_length_1419_cov_3.747067"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1419)
                     /gene="fusA"
                     /locus_tag="PLW80_08205"
     CDS             complement(<1..>1419)
                     /gene="fusA"
                     /locus_tag="PLW80_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPB66530.1"
                     /translation="RNIGIMAHIDAGKTTTTERILYYSGKTYKLGEVHDGEAVMDWME
                     QEQERGITIQSAATTTFWRDHQINIIDTPGHVDFTAEVERSLRVLDGAVAVFCAVGGV
                     EPQSETVWHQADRYHVPRVAYVNKMDRLGADFYSVVAEIEEKFAACPVPVQLPIGKEG
                     GFTGVVDLITMEELDWDSDQGGQNITRSPIRDELAALAAEWREKLVDALASNSDEITE
                     LYLSGEPIPAELIRKALREQTIGRKIVPVLCGASRRNLGVQPVLDAVVDYLPSPTDVP
                     PVQAFNPKKEEHFELPCDPKGNPLGLVFKVQYDREAGPLCYVRMYSGVIKTAGTVYNV
                     GKKKRERVTRILRMHSNKSEAIDEIQAGDIAVFVGMKAAQTGDTVGSEGWPMLLEHMH
                     FPEPVITVAIEPKTASDRDKLKETLDILSKEDPTFQTGDDEDTGQLVIRGMGELHLDV
                     LVTRILKDFKVGAKVGNPQVSYR"
BASE COUNT          200 a          464 c          469 g          286 t
ORIGIN      
        1 gcggtacgat acctgcgggt tgccgacctt tgcgccgacc ttgaagtcct tgaggatgcg
       61 ggtgaccagc acgtcgaggt gcagctcgcc catgccgcgg atgaccagct ggcccgtgtc
      121 ctcgtcgtcg ccggtctgga aggtcgggtc ttccttggac aggatgtcga gcgtctcctt
      181 gagcttgtcg cggtccgagg cggtcttggg ctcgatcgcc accgtgatga cgggctcggg
      241 gaagtgcatg tgctcgagca gcatcggcca gccctcggag ccgacggtgt cgccggtctg
      301 ggccgccttc atgccgacga agaccgctat gtcgccggcc tggatctcgt ctatcgcctc
      361 gctcttgttc gagtgcatgc gcaggatgcg cgtcacgcgc tcgcgcttct tcttgcctac
      421 gttgtatacc gtgccggccg tcttgataac gccggagtac atgcgcacgt agcatagggg
      481 gcccgcttcg cggtcgtact ggaccttgaa cacgaggccg agcgggttgc ccttggggtc
      541 gcagggcagc tcgaagtgct cctctttctt tgggttgaag gcctgcaccg gcggtacgtc
      601 ggtcggggaa ggcaggtagt cgacgaccgc atcgagcacg ggctgcacgc ccaggttgcg
      661 ccgcgacgcg ccgcagagca ccggcacgat cttgcgccct atcgtctgct cgcggagcgc
      721 cttcctgatc agctcggccg ggatgggctc gccggacaga tacagctcgg tgatctcatc
      781 ggagttggag gcgagggcgt cgaccagctt ctcgcgccac tccgccgcta gggccgccag
      841 ctcgtcgcgg atgggcgagc gcgtgatgtt ctgcccgccc tgatccgagt cccagtcgag
      901 ttcctccatc gttatcaggt cgacgacgcc ggtgaagcca ccctccttgc ctatcggtag
      961 ctgcaccggt accgggcagg cggcgaactt ctcctctatc tcggctacca cggagtagaa
     1021 atcggcgccg aggcgatcca tcttgttgac gtaggccacg cgcggcacgt ggtagcggtc
     1081 cgcctggtgc cagaccgtct cgctctgcgg ctccacgccg ccgaccgcgc agaacaccgc
     1141 caccgcgccg tcgagcacgc gcagactacg ctccacctcg gcggtgaagt cgacgtggcc
     1201 gggcgtgtcg atgatgttga tctgatggtc gcgccagaag gtcgtcgtcg cggcgctctg
     1261 gatcgtgatg ccgcgctcct gctcctgctc catccagtcc atgacggcct cgccgtcgtg
     1321 gacctcgccg agcttgtagg tcttgccgga atagtagagg atgcgctccg tcgtcgtcgt
     1381 cttgcccgcg tcgatgtgcg ccatgatgcc gatatttcg
//
LOCUS       DAOOLN010000624         1419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_120570_length_1419_cov_2.524927, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000624 DAOOLN010000000
VERSION     DAOOLN010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120570_length_1419_cov_2.524927"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="PLW80_08210"
     CDS             complement(<1..1254)
                     /locus_tag="PLW80_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HPB66531.1"
                     /translation="MRIDECVYARGGATDSIGDEELAAALIEALAAAGPRHRVLAVPP
                     DGTRSHARAGILLKAAYDYYGDALAAVMPALGTHRPMTDAAVEAVFPGVPPELFRPHD
                     WRRDVVELGRVPAAFVREASEGLCGFDWPAQVNRLVADGGFDLVLSLGQVVPHEVVGM
                     ANYSKNLFVGLGGKDGIDRSHWLGAAYGIERTLGRVDTPVRAVLDYAQERFLGPIPAL
                     YAMTVVGPDGRGGEATRGLFVGGGREAFSRAAALSAEVNIEYLERPADKVVVYLDPGS
                     FKSTWLGNKAVYRTRLAIADSGELLVIAPGVDSFGEDKSIDETIRDYGYRGSEAVRAL
                     VESGELSGDLAGAAHLAHGSPGGRFAVTYAAGGLSAADLAGVGYAWADWREALRRYDP
                     ASLTDGWNEMGGEEIFFVRNPALGLW"
BASE COUNT          215 a          515 c          499 g          190 t
ORIGIN      
        1 ccacagcccg agcgccggat tgcgcacgaa gaaaatctcc tcgccgccca tctcgttcca
       61 gccgtcggtc agcgacgccg ggtcgtagcg gcgcagcgcc tcgcgccaat cggcccaggc
      121 gtagccgacg ccggccaggt cggccgcgga caggccgccg gccgcgtagg tcaccgcgaa
      181 gcgcccaccg ggcgagccat gggccaggtg ggcggcgccg gccaggtcgc cggacagctc
      241 gcctgactcg accagagcgc gcacggcctc ggaaccgcgg tagccgtagt cgcggatggt
      301 ctcgtcgatg ctcttgtcct cgccgaagct atcgacgccg ggggctatca ccaggagctc
      361 gccggagtcg gcaatcgcca gcctggtacg atagacggcc ttgttgccga gccaggtgga
      421 cttgaacgag ccggggtcca ggtagacgac gaccttgtcg gccggccgct ccagatactc
      481 gatattgacc tcggctgaca gggcggccgc cctcgagaag gcctcccggc cgccgcctac
      541 gaacaggccc ctcgtcgcct cgccgccgcg cccgtccggc ccgacgaccg tcatcgcgta
      601 gagcgccggg atggggccca ggaagcgctc ctgggcgtag tcgagcaccg cccggaccgg
      661 cgtatcgacc cggccgaggg tccgctcgat gccgtaggcg gcgccgagcc agtgcgagcg
      721 gtcgatgccg tccttgcctc ccaggccgac gaacaggttc ttcgagtagt tggccatgcc
      781 gacgacctcg tgcggcacca cctggccgag cgacagcacc aggtcgaagc cgccgtcggc
      841 gacgaggcgg ttgacctggg ccggccagtc gaagccgcac aggccctcgg aagcctcgcg
      901 gacgaaggcc gccgggacgc gccccagctc gaccacgtct cggcgccagt cgtgcggcct
      961 gaacagctcg ggaggcacgc cggggaacac cgcctcgacg gccgcgtcgg tcatcgggcg
     1021 atgcgtgccg agggccggca tcaccgccgc gagcgcgtcg ccgtagtagt cgtaggcggc
     1081 cttgagcagg atcccggccc gggcgtggct ccgcgtaccg tcgggcggca ccgcgaggac
     1141 ccggtggcgc ggccccgcgg cggcgagcgc ctcgatcagg gcagcggcca gctcctcgtc
     1201 gcctatggag tcggtcgccc ctccgcgcgc gtatacgcat tcgtcgattc gcatcgtcat
     1261 tcctttaagc cgctatcccc gaggcgacga tagggcgggc gtatgatacc acggaagcgc
     1321 ccgcggtttt aagcccgctt gcgctacggg ccggctggcc gcatactgct ggccatgatc
     1381 gagacgagac gcttcgacga cgaggcggcc tggataggc
//
LOCUS       DAOOLN010000625         1418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_120696_length_1418_cov_2.479090, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000625 DAOOLN010000000
VERSION     DAOOLN010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120696_length_1418_cov_2.479090"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1418)
                     /locus_tag="PLW80_08215"
     CDS             complement(<1..>1418)
                     /locus_tag="PLW80_08215"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB66532.1"
                     /translation="YRPARLELRDVAYVYCFRTPLAPRALYFRAGEAAAFKKDVHAFV
                     ENVKKLVTLHAESGEAKRRREETEARWQKTENQRLAAFEAELAADGFRVVQVQGDEGE
                     VSTDILPLRNGEPVPFESVQAEAESGKIPEAEFSSLRERYFAHMDEMRMLFVELRRGR
                     AELEESVDGLRTQALQPLVHRDIELLASRYEDERTREWIRALERDVMARLYLFQPQPP
                     EAGHRSRLTRYGVNVLTDRDDLSAPPVVYEPNPTYANLFGGLEPAGPDGQGPSRSAYL
                     RVRPGSIVKAAGGFLVMQAEDLVADEEAWTAVKRVLRTGKVAIQPQSGPLGQLAAIKP
                     EPLELDVKIVLIGGEMTYDALYNADPDFQKLFKVCAEFDSTMPRNDDGLREYVSFARK
                     ITEEEGLLEPSADGLAAVAEYGVRLSGSRDRLSTRFSKVADLLREADYRASRSGKDAI
                     DAESVREAERVRSWLAALPEEKV"
BASE COUNT          194 a          489 c          512 g          223 t
ORIGIN      
        1 accttctcct cgggcagggc ggccagccag gagcgcacgc gctcggcctc gcggaccgac
       61 tcggcgtcga tcgcgtcctt gccggagcgg gaggcccggt agtcggcctc gcgcaggagg
      121 tccgccacct tggagaaacg cgtcgacagc cggtcgcggc tacccgacag ccggacgccg
      181 tactcggcca cggcggccag gccgtcggcc gacggctcga ggaggccctc ctcctcggtg
      241 atcttgcgcg cgaacgatac gtactcgcgc agcccgtcgt cgttccgcgg catggtggag
      301 tcgaactcgg cgcagacctt gaacagcttc tggaagtcgg gatcggcgtt gtacagggcg
      361 tcgtaggtca tctcgccgcc tatcagcacg atcttgacgt ccagctcgag gggctcgggc
      421 ttgatcgcgg ccagctggcc gagggggccg gactggggct ggatggccac cttgccggtg
      481 cgcagcaccc gcttgacggc ggtccaggcc tcctcgtcgg cgaccaggtc ctcggcctgc
      541 atgacgagga agccgccggc cgccttgacg atggagcccg ggcggacgcg caggtaggcc
      601 gagcggctgg gaccctgacc gtccggaccg gccggctcca ggccgccgaa gaggttggcg
      661 taggtcgggt tgggctcata gaccaccggc ggagccgaga ggtcgtcgcg gtcggtcagg
      721 acgttgacgc cgtaacgggt caggcggctc ctatggccgg cctctggcgg ctggggctgg
      781 aagaggtaca gccgggccat cacgtcgcgc tccagggcgc gtatccactc gcgggtccgc
      841 tcgtcctcgt agcgtgaggc gagtagctct atatcccggt ggacgagggg ctgcagcgcc
      901 tgggtgcgta gcccgtcgac ggactcctcg agctcggcgc ggccccgcct gagctcgacg
      961 aacagcatgc gcatctcgtc catgtgggcg aagtaccgct cccggagcga cgagaattcc
     1021 gcctcgggga tcttgcccga ctcggcctcc gcctggacgc tctcgaaggg caccggctcc
     1081 ccgttccgca acggcaggat gtcggtcgag acctcgccct cgtcgccctg gacctgcacg
     1141 accctgaacc cgtcggccgc cagctccgcc tcgaaggcgg cgagccgctg gttctcggtc
     1201 ttctgccacc gggcctcggt ctcctcgcgg cggcgcttgg cttcgccgga ctcggcgtgc
     1261 agggtgacga gcttcttgac gttctcgacg aaggcgtgca cgtccttctt gaacgcggcc
     1321 gcctcgccgg ccctgaagta cagggcgcgc ggcgccagcg gcgtgcggaa gcagtagacg
     1381 taggcgacgt cgcgtagctc gagcctggcg ggccggta
//
LOCUS       DAOOLN010000626         1417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_120811_length_1417_cov_2.723201, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000626 DAOOLN010000000
VERSION     DAOOLN010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120811_length_1417_cov_2.723201"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="PLW80_08220"
     CDS             complement(<1..674)
                     /locus_tag="PLW80_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPB66533.1"
                     /translation="MVQFNGMELVRKAVQAMRDEGADKAQASLSRGEQSELNVDAGRM
                     SLYRTTVNVSIRLNAHVGGRKGTVSLNRYDDEAIRTAASEAIAMARSSEPDPANDVSP
                     ARALESFSAGDEEPDSEAMYERLREFVDYAASAYPKTRLEQCILDFSRGQSFYSNSNG
                     AEFEERSGLYGFFAMFTSKDGEAASSFNYSGASHRGLRKPLKAWGDIDELMRQSAEQT
                     RVEPLSG"
     gene            complement(674..>1417)
                     /locus_tag="PLW80_08225"
     CDS             complement(674..>1417)
                     /locus_tag="PLW80_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPB66534.1"
                     /translation="NLARKSKGVYARPGVHDVVLDSDLAGILAHEAVGHTVEADGVLG
                     GSVAADAMGRRVASPLVTLVDFANTALGETCPVPIWVDDEGVEARDAVLIEEGVLRSY
                     MHNKESAAHFGAEPTGNARAYGFYDEPLIRMRNTAILPGESKLQDMIASVDDGYYFLR
                     SSNGQADSTSEFMFGLVMGFEIKGGKLGEAVRDTTISGVAFDMLKTVAAVSDDMSWSC
                     GGMCGKKQSIPVGMGGPAVRCRVNVGGRA"
BASE COUNT          199 a          465 c          501 g          252 t
ORIGIN      
        1 ccggacagcg gctcgacgcg ggtctgctcg gccgactgcc gcatcagctc gtcgatgtcg
       61 ccccaggcct tgagcggctt gcggaggccg cggtgcgagg cgccggagta gttgaagctc
      121 gaggcggcct cgccgtcctt gctggtgaac atcgcgaaga agccgtagag cccggagcgc
      181 tcctcgaatt cggccccgtt cgagttcgag tagaaggact ggccgcggct gaaatcgagg
      241 atgcactgct cgagcctcgt cttggggtag gcggaagccg cgtagtcgac gaactcgcgg
      301 aggcgctcgt acatggcctc gctgtccggc tcctcgtcgc ccgcggagaa gctttcgagg
      361 gcgcgggccg gcgatacgtc gttggctggg tcgggctcgc tcgaacgggc catcgcgatc
      421 gcctcggagg cggcggtgcg tatcgcctcg tcgtcgtagc ggttgagcga taccgtgccc
      481 ttacggccgc cgacgtgggc gttgagccgt atggacacgt tcacggtggt gcggtacagg
      541 ctcatgcggc ccgcgtcgac gttcagctcg ctctgctcgc cgcgcgacag cgacgcttgg
      601 gccttgtcgg ccccctcgtc cctcatggcc tgtacggcct tcctgacgag ctccatgccg
      661 ttgaattgta ccattacgcc cgccctccca cgttgacgcg gcatcggacg gccgggccgc
      721 ccatgcctac gggtatcgat tgcttcttgc cgcacatacc gccgcaggac cagctcatgt
      781 cgtcggacac ggccgctacg gtcttgagca tgtcgaaggc gacgcccgag atcgtcgtgt
      841 cgcggacggc ctcgccgagc ttgccgccct tgatctcgaa gcccatcacg aggccgaaca
      901 tgaattcgct cgtcgagtcc gcctggccgt tggatgagcg caggaaatag tagccgtcgt
      961 cgaccgaggc tatcatgtcc tggagcttgg actcgccggg caggatcgcg gtgttgcgca
     1021 tccggatcag gggctcgtcg tagaagccgt aggcgcgggc gttgccggtc ggctcggcgc
     1081 cgaagtgggc ggccgactct ttgttgtgca tgtaggagcg caggacgcct tcctcgatca
     1141 gcacggcgtc gcgcgcctcg acgccctcgt cgtcgaccca gatcggcacg ggacaggtct
     1201 cgccgagcgc cgtgttggcg aagtcgacca gggtgacgag ggggctggcg acccggcggc
     1261 ccatggcgtc ggcggcgacc gacccgccga gcacgccgtc ggcctcgacc gtgtgcccca
     1321 cggcctcgtg ggccaggatg ccggctaggt cggagtcgag cactacgtcg tggacgccgg
     1381 gtcgggcgta gacgcccttg gacttgcggg ccaggtt
//
LOCUS       DAOOLN010000627         1416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_120926_length_1416_cov_3.116826, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000627 DAOOLN010000000
VERSION     DAOOLN010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120926_length_1416_cov_3.116826"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..>1416
                     /locus_tag="PLW80_08230"
     CDS             238..>1416
                     /locus_tag="PLW80_08230"
                     /inference="COORDINATES: protein motif:HMM:NF020028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66535.1"
                     /translation="MNPATTSSGPAWDLSGEYPAIASAEFAADLAAVKDAIASLEADR
                     PSLAVYVDAAAGLDPVADSGVPASLARTMDRVDEAGATLRNLSVYVNCVLSTNGSDAE
                     AAAMRGYLASLRAALGAAEAPLSLVLKLCPEPFFAAFCSLPGMARFRFALSQERKTRA
                     RALPLPAERAVATMSADGLHAWGGLYDAITGTLRCRALGPDGAESTVGLAKAASLLKR
                     PEPRVRESAWRGINAALSEHRHSLAAILNSIAGWRGAELSMRSHTEPGHYLDESLRQN
                     RLSSATLEAMMGVIRESRELGRRALRAQAGLYGVERLGPWDILAPAPAAEGTNAVGLP
                     FERGLDTVRRAYASVHPSMGDFVSRMARSGRVEGRVEDGKRPGAYCTEFPKSRNQFVY
                     T"
BASE COUNT          181 a          551 c          473 g          211 t
ORIGIN      
        1 ggcgagggcg gcgctcggtc tccagctccc ggagctcgtc ggccagagcc gccgcgccgt
       61 ccggggtcag gtagttccgc gagcccggcg ggacggggat cttgagttcc ggcgcctcgt
      121 cgtcggccgc gtcgtactga tcgtctccca cgaatgccct ggacatggtc tcccccatag
      181 cgactatacc acggtttcgg cttgcccggc ggcggacgcc gtgctacagt cgcccgcatg
      241 aatcccgcaa ccacgtcctc cggcccggcc tgggacctct ccggagagta tccggccata
      301 gcctcggccg aattcgccgc cgacctcgcc gccgtcaagg acgctatcgc ctcgctcgag
      361 gccgaccggc cgtccctggc cgtttacgtc gacgcggctg ccgggctcga cccggtagcc
      421 gattcgggcg tcccggcctc gctggcgcgg acgatggacc gggtcgacga ggccggcgcg
      481 acgctgcgca acctgtcggt ctacgtcaac tgcgtcctgt cgacgaatgg ctccgacgcc
      541 gaggcggccg ccatgcgcgg ctacctcgcc tcgctccggg cagcgctcgg cgcggccgag
      601 gcgccgctct ccctcgtcct caagctatgc cccgagccgt tctttgccgc gttctgctcc
      661 ctgccgggca tggcgcgctt ccgattcgcc ctgagccagg agcgcaagac tcgcgcccgc
      721 gccttgccgc tgccggccga gcgcgccgtc gcgacgatgt cggccgacgg actccacgcc
      781 tggggcgggc tgtacgacgc catcaccggc accctgcgtt gccgggcgct cggcccggac
      841 ggcgccgagt cgaccgtcgg cctggccaag gcggcgagcc tcctcaagcg gccggagccc
      901 agggtccgcg agtcggcctg gcgcggcatc aacgccgccc tgtcggaaca ccgccacagc
      961 ctcgctgcca tactcaactc gatagccggc tggcgcggcg ccgagctatc catgcgctcg
     1021 cataccgagc cgggccacta cctcgacgaa tcgctcaggc agaaccgcct ctccagcgcc
     1081 acgctcgagg cgatgatggg cgtgatccgc gagtccaggg agctgggccg cagggccttg
     1141 cgcgcccagg ccggcctgta cggcgtcgag cgcctcggcc cctgggacat cctggccccc
     1201 gctcccgcgg ccgaagggac gaacgccgtg ggccttccct tcgagcgcgg cctcgatacg
     1261 gtcaggcggg cctacgcgtc cgtgcatccg tcgatgggcg acttcgtctc gcgcatggcc
     1321 cgttccggcc gggtcgaggg ccgcgtcgag gacggaaagc ggccgggcgc ctactgcacc
     1381 gagttcccca agtcccgcaa ccagttcgtc tacacc
//
LOCUS       DAOOLN010000628         1415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_121076_length_1415_cov_2.724265, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000628 DAOOLN010000000
VERSION     DAOOLN010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121076_length_1415_cov_2.724265"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PLW80_08235"
     CDS             complement(<1..619)
                     /locus_tag="PLW80_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HPB66536.1"
                     /translation="MSEYLRLAGIVDEAIALKAAEAIALSDAMAADPEISEREFRSSE
                     AHVAFLRGCGFEVEYPFFGIPTAYNGKVSRGSGGRVALLAEYDALPGIGHACGHNVHG
                     AMSLLAGAGLASVLEELGGELRVVGTPAEETNGAKITMAAGGAFDGLDLALMIHSDND
                     DCRVRYRCLAMDGIEFTFRGKASHAAASPWEGRNALNGAQLFFHAI"
     gene            complement(693..>1415)
                     /locus_tag="PLW80_08240"
     CDS             complement(693..>1415)
                     /locus_tag="PLW80_08240"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HPB66537.1"
                     /translation="KDPSKSLLAGVDMGKLALNHDELVDKKATGRDRLILAWVVGTIG
                     VLIWGVIAKGWYFDELAGLFVIMGIATGLIAGWGPSKIAATFLEGCKDIVFGALVVGL
                     SRGILVVMREAQIIDTVVYAMSQPLAALPRWIAAEGMLVVQTLINFFIPSGSGQAATT
                     MPIMAPLSDLLGISRQTAVLAFQYGDGFSNLLWPTTLLPVVCSIAKIPIQKWWRFFVP
                     FFGLLLVAQVIFIAVSVMIGLA"
BASE COUNT          249 a          479 c          470 g          217 t
ORIGIN      
        1 cgatggcgtg aaagaacagc tgcgccccgt tgagcgcgtt gcgaccctcc cagggcgagg
       61 cggccgcgtg cgaggccttg ccacggaacg tgaactcgat gccgtccatc gccaggcagc
      121 ggtagcgcac ccggcagtcg tcattgtccg aatggatcat cagcgcaagg tccagcccgt
      181 cgaaggcgcc gcccgcggcc atggtgatct tggcgccgtt cgtctcctcg gccggcgtgc
      241 cgacgacccg gagctcgccg ccgagttctt ccaggacgga cgccaggcca gcgccggcca
      301 agaggctcat cgcgccgtgc acattatgcc cgcaggcgtg gcctatgccc ggtagggcgt
      361 cgtactcggc caggagcgcc acgcgcccgc cggagccgcg ggacaccttg ccgttatagg
      421 ccgtcgggat gccgaagaag gggtactcca cctcgaagcc gcagccccgg aggaacgcga
      481 cgtgagcctc gctgctccgg aactcccgct ccgatatctc cggatccgcc gccatcgcgt
      541 cgctcagggc gatcgcctcg gcggctttca gcgctatagc ctcgtcgacg atcccggcca
      601 gcctcagata ctcgctcacg tcgtctcctt tccgccgcgg cgcccctccg tccgtgaagg
      661 gcgccgtcgt cagtgtagaa cggttctggc cgtcaggcca gccctatcat gaccgagacc
      721 gcgatgaaga tgacctgggc gaccaagagg aggccgaaga acgggacgaa gaagcgccac
      781 cacttctgga tcgggatctt ggcgatggag cagacgaccg gcaatagcgt cgtcggccac
      841 agcaggttgg agaagccgtc gccatactgg aacgccagca ccgcggtctg cctcgagatg
      901 cccaggaggt cggacagcgg ggccatgatc ggcatggtcg tggcggcctg gcccgagccg
      961 gacgggatga agaagttgat cagggtctgc acgacgagca tgccctcggc ggctatccat
     1021 cgcggcagcg cggcgagcgg ttgcgacatg gcgtagacga ccgtgtcgat gatctgcgcc
     1081 tcgcgcatga cgacgaggat accgcgcgac aagccgacga ccagcgcgcc gaagacgatg
     1141 tccttgcagc cctcgaggaa ggtggcggcg atcttgctgg ggccccagcc ggcgatcagg
     1201 cccgtggcaa tgcccatgat gacgaacagg ccggccagct cgtcgaagta ccagcccttg
     1261 gcgatgacgc cccagatgag tacgcctatg gtgccgacga cccaggccag gatcagccgg
     1321 tcgcgtccgg tagccttctt atccaccagc tcatcgtggt tcagcgccag cttgcccata
     1381 tcgacgccgg cgaggaggct cttgctcggg tcctt
//
LOCUS       DAOOLN010000629         1414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_121183_length_1414_cov_3.319352, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000629 DAOOLN010000000
VERSION     DAOOLN010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121183_length_1414_cov_3.319352"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..839
                     /locus_tag="PLW80_08245"
     CDS             12..839
                     /locus_tag="PLW80_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPB66538.1"
                     /translation="MPFVRNALAAGLLSAVLFGVLGAVVTVKRIAGLAGAIAHAVLGG
                     IGLSLFLAAKGIAPGLPPIVGALVFAVIAAAVIGFVSLRAKQREDTVINAIWAIGMSV
                     GVLFIAKTPGYADPMSYLFGNILLVSGRDLALLAALDAVVLFLAWRFYPQIEASAFDE
                     EFAKVRGVPTDAVFVATLAVTAVAVVLLQTFVGIVMVIAMLTLPSGTAGFAARSLAAM
                     MAGGAALSAAFTVAGLAASWSLDLPAGAVVVVIAGAAFLAVSAAVAVKARKARGGRA"
     gene            836..1216
                     /locus_tag="PLW80_08250"
     CDS             836..1216
                     /locus_tag="PLW80_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPB66539.1"
                     /translation="MTRARAAVLAALESTVEPLSAAGVASRMAGACDQATVYRALHHL
                     EEAGLAESFVLHCAMHGVERYYAATSSPHRHWFHCESCHRFVDLGACRLGGLVSELER
                     ERGLRVTRHTMYLTGVCGDCAARE"
BASE COUNT          174 a          504 c          506 g          230 t
ORIGIN      
        1 tcgacccgac gatgcctttc gtccgcaacg ccctcgcggc cgggctgttg tcggcggtac
       61 tgttcggcgt gctcggcgcg gtcgtcaccg tgaaacgcat agcgggcctc gccggagcca
      121 tagcgcacgc ggtgctcggc ggcataggcc tgtcgctgtt cctggcggcc aagggcatag
      181 cgcccggcct gccgcccatc gtcggcgcgc tggtcttcgc ggtgatcgcc gcggcggtga
      241 taggcttcgt gtcgttacgc gccaagcagc gcgaggacac ggtgatcaac gcgatctggg
      301 ccattggcat gagcgtcggc gtgctgttca tcgccaagac ccccggctac gccgacccga
      361 tgagctacct gttcggcaac atcctgctcg tatccggccg ggacctcgcg ctgcttgccg
      421 cgctcgacgc ggtcgtgctg ttcctcgcct ggcgcttcta tccgcagata gaggcgagcg
      481 ccttcgacga ggaattcgcc aaggtgcgcg gcgtgccgac cgacgccgtc ttcgtggcga
      541 ccctcgccgt cacggccgtt gcggtcgtgc tgctccagac cttcgtcggc atcgtgatgg
      601 tcatcgcgat gctgaccctg ccctcgggca ccgccggctt cgccgccagg agcctggcgg
      661 cgatgatggc cgggggcgcc gcgctctcgg ccgccttcac ggtcgctggc ctggccgcca
      721 gctggtcgct cgacctgccg gccggcgccg tcgtcgtcgt gatagccggc gccgctttcc
      781 tcgcggtatc ggcggcggtc gccgtcaagg ccaggaaggc ccgcgggggc cgcgcgtgac
      841 ccgggccagg gccgcggtgc tggccgccct ggagtcgacc gtcgagccgc tatcggcggc
      901 tggcgtagcc tcgcggatgg ccggcgcctg cgaccaggcc acggtgtacc gtgcgctcca
      961 tcacctcgag gaggcagggc tggcggagtc gttcgtgctg cactgcgcga tgcacggcgt
     1021 cgagcgctac tacgccgcga ccagctcgcc ccaccggcac tggttccact gcgagagctg
     1081 ccatcgcttc gtcgacctcg gcgcgtgcag gctgggcggg ctcgtctccg aactggagcg
     1141 cgagcgcggc ctacgcgtca cgaggcacac gatgtacctg accggcgtct gcggcgattg
     1201 cgccgcccgg gagtgaggac ggccccgggc cgagccctga ggccgtcccg gggctgggga
     1261 tactcaggct atcgccgagc ggtgacgctt ggcggcggga tggcgcaggc gccgctccag
     1321 ccagtcgagc cgagcgacga gctcctccgg gaacccgtcg tggaagctac cgaagaaggc
     1381 gcggataccc ggcacgtcgg ccagccattc gtcc
//
LOCUS       DAOOLN010000630         1414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_121195_length_1414_cov_2.972038, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000630 DAOOLN010000000
VERSION     DAOOLN010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121195_length_1414_cov_2.972038"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="PLW80_08255"
     CDS             <1..1244
                     /locus_tag="PLW80_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66540.1"
                     /translation="AGVSPESSASLFYSLDRSVPFFMRGNAGLASALKLYGRGVASVR
                     LSPGSVRLELSAVPAAKGVKATLPGFPIDAGGRMGSDPVLGRTASGVPVAFWTSGQKL
                     RGVDLSSGARYEAELDAPGWLALDHGNGRMAALWAVSDRGTVYRLDATLRPLEGFPLV
                     TGQKVSGPCVASFGRLVVPVASEPALMLVSADGSTRFSSAFGSRQRSAPAVSGYALAA
                     LPRSFDSELYLMDLEGMALPGWPVPLPGIASAAPAFAGADTAADGRALALTEAGQLLA
                     YAIDAAASPGFPVPLEGSFDAAPVWAPGWRSVYALSVEGTLYRVGLDGSTLGSVRVGR
                     QSARGAAVTAYDADGDGREELYVSGGGDALYAFSGDLSPLPGYPVSGAGAPRFIDIDG
                     DGTDDMVVRGADDTLRAYSGR"
BASE COUNT          162 a          498 c          513 g          241 t
ORIGIN      
        1 cggccggcgt ctcgcccgag tcatcggcct cgctgttcta ttcgctcgac cggagcgttc
       61 cgttcttcat gcgcggcaac gcggggctcg cctcggcgct caagctgtac gggcgggggg
      121 tcgcctcggt ccgactatcg cccggttccg tcaggctcga gctgtcggcg gtaccggccg
      181 ccaagggcgt taaggcgacg ctgccgggct tccctatcga cgcaggcggc aggatgggct
      241 cggacccggt cttaggcagg acggcctccg gcgtgccggt ggccttctgg acgagcggcc
      301 agaagctgcg tggggtcgac ctgtcgtccg gggcgcgcta cgaggccgag ctggacgcgc
      361 caggctggct cgccctcgat catgggaacg gccgcatggc cgcgctctgg gccgtctccg
      421 atcgcggcac cgtctatcgc ttggatgcga ccctgaggcc gctcgaaggc ttcccgctcg
      481 tgaccgggca gaaggtctcc ggaccctgcg tcgcgtcgtt cggtcgcctg gtcgtgccgg
      541 tggcctcgga gccggcgctg atgctcgtat cggccgacgg ctcgacccgc ttctcgagcg
      601 ccttcggctc gcgacaacga tcggcccctg ccgtatccgg atacgcgctg gcggccctgc
      661 cgcgctcgtt cgattccgag ctctacctga tggacctcga gggcatggcg ctacccggct
      721 ggcccgtgcc gttacccggt atcgcctcgg cggcgccggc cttcgccggg gcggataccg
      781 cggccgacgg tcgcgcgctg gcgttgaccg aggcgggcca gcttctggcc tacgccatcg
      841 acgcggcggc gtcgcccggc ttcccggtgc cgctcgaggg cagcttcgac gcggctccgg
      901 tatgggcgcc cggctggcgc tccgtctatg cgctgtcggt cgaggggacg ctgtaccggg
      961 tgggcctgga cggttcgacg ctcggctcgg tcagggtcgg ccgccagtcg gcccgcggcg
     1021 ccgccgttac ggcctacgac gccgacggcg atggtcgcga ggagctgtac gtatcgggcg
     1081 gcggcgacgc gctgtacgcg ttctcgggtg acttatcgcc attgcccggc taccccgtct
     1141 ccggggctgg cgctccccgt ttcatcgaca tagacggcga cggaacggac gatatggtcg
     1201 tgcgcggcgc cgacgatacc ctgcgcgcct actccggtcg ctgaaaggaa tgacgtgatg
     1261 aagagaccgc taaggatcgc ggccgcgttc gcggcggccg tcgccgccgg gttcgccgcc
     1321 gcctcgtgcg cctcgatcca gagggacgtg tactacgacg ctcccgccgg cgaggatccg
     1381 gccgagctcg ccgagataga gtcgagcctc gtgc
//
LOCUS       DAOOLN010000631         1414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_121214_length_1414_cov_2.678440, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000631 DAOOLN010000000
VERSION     DAOOLN010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121214_length_1414_cov_2.678440"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PLW80_08260"
     CDS             complement(<1..459)
                     /locus_tag="PLW80_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66541.1"
                     /translation="MKSPSLATAFDTAIADTARRIDTLLAGNPDQTELEALQTELAAL
                     IRNRRDHLYSLVDARAIVSQVFDEPFSETRRQKAAELEGQVLSLIEQYHREADETKEV
                     LRSDYDLLVDVLAFLRFFKHDDLPTSETEAPAPRLIWTGKRGLTRQAFVEA"
     gene            complement(539..1126)
                     /locus_tag="PLW80_08265"
     CDS             complement(539..1126)
                     /locus_tag="PLW80_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66542.1"
                     /translation="MPVSKLRLPRKDLIPPLPDITELARQLQERLEGLQDPTLRAEEI
                     GITLAELDRIKQHGDTLYRRLNDEGALDEPDAYWKYSEELDRFRHELLIERDRELLAA
                     RRPEPDQSATRKPRQTPLPAFLAEKDDRQARNRERTDLPDTMNMAQLASYLGVSTQWA
                     YQNYKRLAIPYAVIGCLRRFRKDKVDAWWEAQSRQ"
     gene            complement(1113..>1414)
                     /locus_tag="PLW80_08270"
     CDS             complement(1113..>1414)
                     /locus_tag="PLW80_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66543.1"
                     /translation="RDRPVNEVTIRRGWERALELIGIPERERLKRNLLLHGARHAYTT
                     RLLDSGELSPAEVAKLTRHKDLSMLSRYGGHLRDETIEKGRKALSFKKEADHASQ"
BASE COUNT          240 a          376 c          453 g          345 t
ORIGIN      
        1 ggcttctacg aaggcctgcc gcgtcaagcc acgcttgccg gtccagatca ggcgcggtgc
       61 gggggcctca gtttctgacg tcggtaggtc gtcatgtttg aagaagcgca ggaaggcaag
      121 gacatctacc agtagatcgt aatccgagcg taagacctcc ttggtctcat cagcttcccg
      181 atggtattgt tcgattaacg ataagacctg gccttcgagt tcggccgcct tctggcgacg
      241 ggtttcagag aatggttcgt cgaagacctg gctgacgatg gctcgggcgt cgacgaggct
      301 atataggtga tcccgccgat ttctgataag ggcggcaagt tcggtctgta aggcctctag
      361 ctcggtttgg tcagggtttc cggcgagaag cgtatcgatt cgacgggcgg tatcggctat
      421 ggctgtatcg aaggccgtgg ctaaagatgg tgacttcata tcgacagttt atcatctgcg
      481 atatgatggc gccactgcct tataggcatc ggcaaaatac ctataaggcg gtggtcggtt
      541 actgcctgct ttgagcctcc caccaagcgt cgactttgtc cttgcggaag cgcctgaggc
      601 atccgattac cgcgtagggt atggcgaggc gtttgtagtt ctgataggcc cactgggtgc
      661 tcactcccag gtaggacgcc agttgcgcca tgttcatggt gtcggggaga tcggtccgct
      721 cacggttcct ggcttgccga tcgtccttct cggccaggaa ggcgggcagc ggcgtctggc
      781 gcggtttccg ggttgcggat tgatccggtt cggggcggcg ggctgcgagg agctcgcgat
      841 ctcgttcgat gagaagctca tgccggaatc ggtctaactc ttcggaatac ttccagtacg
      901 cgtcaggttc atccagggcc ccttcatcgt ttagcctgcg gtagagggta tcgccatgct
      961 gcttgatgcg atccagctcc gcgagggtga tcccgatctc ttccgctcga agcgtcgggt
     1021 cttgaagtcc ttcgagccgt tcttgaagct gtcgcgccag ttcggtgatg tccgggagcg
     1081 gcgggataag gtctttcctg ggtagcctga gtttactgac tggcatggtc tgcctccttc
     1141 ttgaagctga gggccttgcg ccccttctct atggtctcgt ctcggagatg gccgccgtag
     1201 cgggatagca tggaaagatc cttgtggcgg gtcagcttgg ccacttcggc cggtgagagc
     1261 tccccggaat ccaggagccg ggtagtgtag gcgtggcgcg cgccatgaag gagcaggttt
     1321 cgtttgaggc gttcccgttc cggaataccg ataagctcta gcgctctttc ccaacctcgg
     1381 cgaatcgtta cttcattgac gggtcgatcc cgcg
//
LOCUS       DAOOLN010000632         1413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_121352_length_1413_cov_2.432253, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000632 DAOOLN010000000
VERSION     DAOOLN010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121352_length_1413_cov_2.432253"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PLW80_08275"
     CDS             complement(<1..777)
                     /locus_tag="PLW80_08275"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPB66544.1"
                     /translation="MAVLGVAAIALGLALRQGAARRSREPKPDGAGISIGFSMDSFVE
                     ERRARDRDVFVAMARDLGATVNVQIGDEDPSIQEEQILFLLDSGVDVLVVIPSDPAGL
                     GRAVAEAKRRGVPILAYDRLILNGNVDLFLAFDAERSGELQAEAFLASQGGGRVALYN
                     GRRSDPNAAAMRAGVMNTLRPDIDEGRVIVVADYESAERRSEEAYQIVDSMLEAGTTF
                     DAVLAVDDMAAEAIIRALSVRRLAGSVFVAGADADLAACQR"
     gene            complement(846..>1413)
                     /locus_tag="PLW80_08280"
     CDS             complement(846..>1413)
                     /locus_tag="PLW80_08280"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPB66545.1"
                     /translation="HRAVIFLGCAGILLLCLTVALIARSITGPLRDMAAAAGAIAWSL
                     RHDPEAVVEAAAAVSEGAAPRAAFNSGKPGSGYGATGRVADALRCIEERYAEQISLED
                     VATQVGLSATHLSRVLSNETGKSFIEHLSDYRIARAKAELASGRMSVKEVGAAVGYLD
                     PNYFSRAFKRVTGLTPSDYAKSAGRGDI"
BASE COUNT          212 a          496 c          478 g          227 t
ORIGIN      
        1 gcgctggcag gccgctaggt cggcgtccgc gccggccacg aagaccgacc cggctagccg
       61 ccgcaccgac agggcgcgga tgatcgcctc ggcggccata tcgtcgaccg cgagtaccgc
      121 gtcgaaggtc gtgcccgcct caagcatcga gtcgacgatc tggtaggctt cctcgctcct
      181 acgctcagcc gactcgtagt cggcgacgac gatgacccgg ccctcgtcga tgtccggcct
      241 cagggtgttc atgacgccgg cccgcatggc cgcggcattc gggtcggatc gcctgccgtt
      301 gtagagcgct accctcccgc cgccctgaga cgccaggaag gcctcggcct ggagctctcc
      361 ggaccgttct gcatcgaagg ccaggaacag gtcgacgttg ccgttcagga tgagcctgtc
      421 gtaggcgagg atcgggacgc ctctacgctt ggcctcggcg acggcccggc cgagaccggc
      481 ggggtcgctc ggtatgacga ccagaacgtc gacgccggaa tcgagcagga acagtatctg
      541 ttcctcctgg atggacggat cctcgtcgcc gatctgcacg ttgaccgtgg ctccaaggtc
      601 gcgcgccatg gcgacgaaga cgtcgcggtc gcgggcgcgg cgttcctcga cgaacgaatc
      661 catggagaat cctatcgata tgcccgcccc gtcaggcttg ggctccctgg agcgccgcgc
      721 cgctccctgc ctcagggcca gtccgagcgc tatagccgcc acgcctagga ccgccacgcc
      781 tagggccgcc gcggccatga tcgctcgccg ccgcctcgcg gccgcgcctt tggttccccc
      841 gccgatcata tgtcaccccg accggcagac ttggcatagt cggacggcgt cagaccggtg
      901 acgcgcttga aggcccggct gaagtagttc gggtcgaggt agcctacggc ggctccgacc
      961 tccttgacgc tcatgcggcc cgaggccagc tccgccttgg ccctggctat cctgtaatcg
     1021 gatagatgct ctatgaacga ttttcccgtc tcgttcgata gcaccctcga caggtgggtg
     1081 gccgagaggc cgacctgggt cgccacgtcc tcgagcgata tctgttcggc gtagcgttcc
     1141 tcgatgcacc gcagggcgtc ggctacgcgg ccggtggcgc cgtagccgga gccaggctta
     1201 cccgagttga aggcggccct cggcgccgcg ccctcggata cggccgcggc cgcctcgacg
     1261 acggcttccg ggtcgtggcg gagcgaccac gctatggccc cggccgcggc ggccatgtcc
     1321 cgcagcggcc cggtgatgga gcgggcgatg agcgccacgg tgaggcagag gagcaggatg
     1381 cccgcgcatc ccaggaagat gacggcccgg tgc
//
LOCUS       DAOOLN010000633         1411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_121568_length_1411_cov_3.370206, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000633 DAOOLN010000000
VERSION     DAOOLN010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121568_length_1411_cov_3.370206"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1411
                     /locus_tag="PLW80_08285"
     CDS             <1..>1411
                     /locus_tag="PLW80_08285"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66546.1"
                     /translation="SIDGESGYASIYVNGAPNAGPLAFSGIPSDPTIRIGVRMTNSGF
                     FQGSFDDLRVYAGALAADEIAAIRADGYFSGGRGTAASPYLVATPMDLYNVRRFPAAH
                     FRQTADIDLSANAMTSTWAPIVGPFTGTYDGGGRSVSNVALGALSKAGSGFFEQISPG
                     GVVRNLTMTTVSGSSSAENLGALAGIITSATISDITITGIDLTGTIYIGGLAGTCSNS
                     ALTRINVEGAIASSATLATYLGGMAGQLSGTAVSCVAAVNVGPAAGTTSPNTQQVGGF
                     AGSIREFSDIVDCHARGNILYGGAGNGLHIGGFAGQISQQAALQIQVRACTSSGSVAT
                     NEADYVGGFSGQLYATSNAIDLLIDDCSSTSTVTAPNGQYIGGFAGLAGIHTSGAGKN
                     RITSSRSTGTVSANGTNSGSFVGGFIGYAKEGTVIEECSSTSNVSAKSQFIGGFAGAL
                     GSDAAAVGAPGSIKRSYATG"
BASE COUNT          282 a          444 c          430 g          255 t
ORIGIN      
        1 ctctatcgac ggcgaatcgg gctatgcctc gatctacgtg aacggcgcgc cgaacgccgg
       61 cccgctagcc ttttctggca ttccaagcga tccgactata cgtatcggcg tgcgcatgac
      121 gaactcgggt ttcttccagg gctcgttcga cgacctccgc gtctacgcgg gcgccctagc
      181 ggccgacgag atcgcggcca tccgcgccga cggctacttc tccggcggac gaggtacggc
      241 cgcctcgccc tacctcgtcg cgacgccaat ggacctgtac aacgtgcgcc gcttccccgc
      301 ggcccacttc aggcagacgg cggacattga cctgagcgcc aacgccatga ccagcacctg
      361 ggcgcctatc gtcggtccat tcaccggcac ctacgacgga ggcggcagga gcgtctcgaa
      421 cgtcgcgctc ggcgcactaa gcaaggcggg ttccggtttc ttcgagcaga tatcccccgg
      481 aggcgtcgta aggaacctta cgatgactac ggtgagcggt tcgtcctcgg cagagaatct
      541 aggtgcattg gccgggatca taacgagcgc cactatctct gacatcacga taaccggcat
      601 agatctgacc ggcactatct atatcggcgg cttggcgggt acatgctcga acagcgcatt
      661 gaccaggatc aacgtcgagg gagccatagc gtcctccgcc acgctcgcga cgtaccttgg
      721 aggcatggcg ggacagctga gcgggacggc cgtttcatgc gtagccgccg tcaacgtagg
      781 cccggccgcc ggaacaacgt ctcctaacac acaacaggtt gggggcttcg ccggatcgat
      841 acgagaattc tcggacatcg tagattgcca tgccaggggg aacatactct acggcggcgc
      901 ggggaacggg ttgcacatag gcggctttgc cggccagata tcgcagcagg cagcgctaca
      961 gatacaagtg cgtgcctgca cctcgtccgg ctcggtggct actaacgaag ccgattacgt
     1021 cggaggcttc tcaggtcaat tgtatgcaac ttccaacgct atcgacttac taatagacga
     1081 ctgctcgtcc acgagtaccg ttaccgctcc taatggacaa tatatcggcg gcttcgcggg
     1141 gctggcgggt attcatacaa gcggtgccgg taagaacagg attacctcgt ctcgctcgac
     1201 cgggacggtc agcgccaacg gtacgaactc gggcagtttc gtcggcgggt tcatcggcta
     1261 cgcgaaggaa ggcacggtta tcgaggagtg ctccagtacg agcaacgtga gcgccaagag
     1321 ccagttcata ggcggcttcg ccggggccct tggctccgac gccgccgccg tcggggcccc
     1381 cggtagcata aaacgaagct acgccacggg a
//
LOCUS       DAOOLN010000634         1406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_122254_length_1406_cov_3.764619, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000634 DAOOLN010000000
VERSION     DAOOLN010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122254_length_1406_cov_3.764619"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..652)
                     /gene="pth"
                     /locus_tag="PLW80_08290"
     CDS             complement(77..652)
                     /gene="pth"
                     /locus_tag="PLW80_08290"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPB66547.1"
                     /translation="MIKLYAFLGNHGREYSGNRHNVAWLFLESLPFYPSLQWKRGFKG
                     RWAQRETPLGRTWFIVPETYMNLSGDSVAEIARFYKVEPGDVLVTHDELELGFGYFGF
                     KRGGGLGGHNGLRSMKERLGTADFMRLRFGIGRPNHDDVAGYVLSDFSRDEREKLECS
                     VFPRAATALELCAERGFDEALAAYTKVDALA"
     gene            734..>1406
                     /locus_tag="PLW80_08295"
     CDS             734..>1406
                     /locus_tag="PLW80_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HPB66548.1"
                     /translation="MSDTTYVIGHKNPDTDSVVSAAAYAHLKRELGSAGCVAARAGKM
                     SPQAEYVFERFGVPFPAFIPDLVPKVEYYMGGPAIVVDRGTPLWDALATMNQSGQKAL
                     PIVDGDGRYRAMLHYSSFAQNILKKINPHKKAVIPTSVGRLEATIKAQQVCAFAPDEM
                     FKARVLVAALETDSFKRHLDAEAVDSVIVIVGDRADVQRYSIESKVRAMIVTNGALLS
                     RELKEL"
BASE COUNT          258 a          501 c          433 g          214 t
ORIGIN      
        1 caaggtccgc gtccagcccg actggaagaa ggacgacaag ctcctcaagc ggctcatcca
       61 gtaggcccgg ccggcctcag gccagcgcgt cgaccttggt ataggcggcc agcgcctcgt
      121 cgaagccgcg ctccgcgcat aactcgaggg cggtcgcggc gcgcgggaac accgagcact
      181 cgagcttctc ccgctcgtcg cgggaaaagt ccgacagcac gtagccggcc acgtcgtcgt
      241 ggttcggccg gcctatgccg aagcgtagcc gcatgaagtc cgcggtaccg agccgctcct
      301 tcatcgacct gaggccgttg tgcccgccga gcccgccgcc gcgcttgaag ccgaaataac
      361 cgaagccgag ctccagctcg tcgtgcgtca cgagcacatc gcctggctca accttgtaga
      421 accgggcgat ctccgcgacg ctgtcgccgg acaggttcat atatgtctcc gggacgatga
      481 accaggtgcg gccgagcggc gtctcccgct gggcccagcg gcccttgaag ccgcgcttcc
      541 actggagcga cggatagaac ggcagcgact cgaggaaaag ccaggccaca ttatgcctgt
      601 tgccggagta ttcccggccg tggttgccca ggaaggcgta cagcttgatc atacaggcca
      661 cagcatagcg attcggcccg cctttggcca tccggtttga ccaggctccg ccccgggcga
      721 tacactgtcc gacatgagcg acaccaccta cgtgataggc cacaagaacc cggacaccga
      781 ctccgtggtc tccgccgccg cctacgccca cctcaagcgc gagctcggct ccgcgggctg
      841 cgtcgcggcc cgcgccggca agatgagccc ccaggccgag tacgtcttcg agcgcttcgg
      901 cgtgccattc cccgcgttca tcccggacct cgtgcccaag gtcgagtact acatgggcgg
      961 tccggccatc gttgtcgacc gcggcacgcc gctctgggac gccctcgcga cgatgaacca
     1021 gtcgggccag aaggccctgc ccatcgtcga cggggacggc cgctaccgcg ccatgctcca
     1081 ttacagctcg ttcgcgcaga acatcctcaa gaagatcaac ccgcataaaa aggcggtcat
     1141 cccgacgagc gtcggccggc tcgaggcgac cataaaggcc cagcaggtct gcgccttcgc
     1201 cccggacgag atgttcaagg cgcgagtgct cgtggcggcc ctcgagaccg acagcttcaa
     1261 gcgccacctc gacgccgagg cggtcgactc ggtcatcgtc atcgtcggcg accgcgccga
     1321 tgtccagcga tactccatcg agtccaaggt cagggcgatg atcgtgacga acggcgccct
     1381 cctgtcgcgc gagctcaagg aactgg
//
LOCUS       DAOOLN010000635         1406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_122292_length_1406_cov_2.757217, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000635 DAOOLN010000000
VERSION     DAOOLN010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122292_length_1406_cov_2.757217"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..1028
                     /locus_tag="PLW80_08300"
     CDS             162..1028
                     /locus_tag="PLW80_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66549.1"
                     /translation="MKRLVVVAIALAAVSCTTRPASLPETGWEATAPAQVRKEYQREV
                     KVPVVVKETVSYADGVVDRIVTHEYDEGLMYLLASYSSKPSSLALASRVSYGYADGRL
                     ASKAAYAEDGSASSRVEYAYDADGNPIRETTLDGKGAVQSVSEWAWEKGLRTSWRVSS
                     GDGTVLARTDYYYDDGSLASARLYDGSGGPRGRVEYGYDADGALMSTRYYSADGKSDG
                     RIEYRRDGNRLVEESVYRADGRLERKLTYGYGLDGELLRKTLHDGSGKAREHVDYEYV
                     FRIATETVVYYE"
     gene            1055..>1406
                     /locus_tag="PLW80_08305"
     CDS             1055..>1406
                     /locus_tag="PLW80_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66550.1"
                     /translation="MRIIRSAALAAALAIAISPAFSGEAARVYGRILAEAGSVQEKYA
                     AATAASSVDDPDLAPYLADALDWTLGARSTVRSGPERETYEKLTRVLLKRLGEARYAN
                     AAASAMRAVGDSTDP"
BASE COUNT          266 a          439 c          469 g          232 t
ORIGIN      
        1 acctgcctag gcgcccatgg atcgtattca ggctttggac ctagctcagg atgaaggtca
       61 tcagcctctg gtaccgtatc cgcgggaaat agtctttttt ctatacttcc aacactaaaa
      121 gtcggctata tcatctcagt ttccactgat cggaggaacg aatgaagcga ttagtcgtgg
      181 tcgcgatcgc cctggcggcg gtctcgtgca ctacgcggcc agcctcgcta ccggaaaccg
      241 ggtgggaagc gacggccccg gctcaggtgc gcaaggaata tcagcgagag gtcaaggtcc
      301 ccgtcgtcgt caaggagacg gtatcgtatg cggacggcgt cgtcgatagg atcgtgactc
      361 acgagtacga cgaggggctg atgtatctcc tcgcctcgta cagctccaag ccgtcgtcgc
      421 tggcgctcgc gagccgcgtt tcctacgggt atgcggacgg gaggctcgcg tccaaggccg
      481 cctatgccga ggacggctcc gcgtcgtccc gcgtcgagta tgcctatgac gccgacggca
      541 atcccatcag ggagacgacg ctcgacggta agggcgccgt tcagtccgta tcggaatggg
      601 cctgggagaa gggcctccgg acatcgtggc gcgtctcgtc cggcgacgga accgtcctgg
      661 ccaggactga ctattactac gacgacggga gcctcgcgtc ggccaggctg tacgatggct
      721 cgggcggccc gcgcggccgg gtcgagtacg ggtacgacgc cgacggcgcg ctgatgtcga
      781 ccaggtatta tagcgccgac gggaaatccg acgggcgcat cgagtaccgt cgcgacggca
      841 accggctggt ggaagagagc gtgtaccgcg ccgatggcag gctcgagcgc aagctgacct
      901 acggctatgg cctcgacgga gagctactac gtaagacgct tcacgacggc tccggcaaag
      961 ccagggaaca cgtcgactac gagtacgtgt tcaggatcgc gacggagacc gtcgtctact
     1021 acgaataatg ggcgaagccc gagtgaggaa caccatgcga atcatcagga gcgcggcgtt
     1081 agccgccgcg ctggccatag ccatatcgcc cgcgttctcc ggcgaggccg ccagggtcta
     1141 cggccgcatc ctggccgagg ccggctcggt gcaggagaag tacgccgccg ccacggcagc
     1201 gtcgtcggtc gacgacccgg acctggcccc atacctggcc gacgcgctcg actggaccct
     1261 cggcgcccgt agcacggtcc ggtcgggtcc cgaacgcgag acctatgaga agctgacgag
     1321 ggtcctactc aagcggctcg gcgaggctcg ctacgccaac gcggcggcgt cggcgatgcg
     1381 tgccgttgga gactcgaccg acccgc
//
LOCUS       DAOOLN010000636         1405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_122444_length_1405_cov_2.267407, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000636 DAOOLN010000000
VERSION     DAOOLN010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122444_length_1405_cov_2.267407"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PLW80_08310"
     CDS             <1..695
                     /locus_tag="PLW80_08310"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPB66551.1"
                     /translation="NGDALFMPQFHSARDIHDVHYVSEPTKAKAIEEPYLSRVATTHE
                     NGGATGSRGWRYGFDREFTKRLVLRSQGTVLSARQLPTAKVPGKYFGIARCFRYDKVD
                     ATHLSDFYQTEGIVLGDDVNLRTLLGFLEMFAVEVAGAKEVKYVPGYFPFTEPSVEVH
                     IKHPVLGWFELGGAGIFRPEVTEPLGVSVPVLAWGIGIDRMALMALGLNDLRELFSYD
                     IENVRLRRALLK"
     gene            735..>1405
                     /locus_tag="PLW80_08315"
     CDS             735..>1405
                     /locus_tag="PLW80_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPB66552.1"
                     /translation="MPKIEVNENLFFELVGRRMDAAELEPALETAKAELDGWDRSGPD
                     AERVIKIELNDTNRPDLWSTAGVARQLRVKLGGAIPEYPFFSREGDQRPTDYRVVVDQ
                     SIRELRPYMAAFVISGKAISDAMLRDAIQTQEKLCSNFGRKRRSVSMGMYRIELITWP
                     VAYKGVDPDAVSFVPLQSDESMTLRRIAAEHPKGKEYGHIIADSPVYPILMGADGGVL
                     SMAPI"
BASE COUNT          259 a          479 c          439 g          228 t
ORIGIN      
        1 ggaacggcga cgctctgttc atgccccagt tccactcggc ccgcgacatt cacgacgtcc
       61 actacgtctc cgagccgacc aaggccaagg ctatcgagga gccctacctt tcgcgcgtcg
      121 cgaccacgca cgagaacggc ggggccaccg gctcccgcgg ttggcgctac ggcttcgacc
      181 gcgagttcac caagcgcctc gtgctgcgca gccagggcac ggtgctgtcg gccaggcagc
      241 tgcctaccgc caaggtgccc ggcaagtact tcggcatagc ccgttgcttc cgctacgaca
      301 aggtcgacgc gacccacctg tccgacttct accagaccga gggcatcgtg ctcggcgacg
      361 acgtaaacct gaggacgctg ctcggcttcc tcgagatgtt cgccgtagag gtcgcgggcg
      421 ccaaggaagt gaagtacgtc cccggctact tcccgttcac cgagccgtcg gtcgaggtgc
      481 acataaagca cccggtgctc ggctggttcg agctcggcgg cgccgggata ttccgcccgg
      541 aggtcaccga gccacttggc gtcagcgtgc cggtgctcgc gtggggcata ggcatcgacc
      601 gcatggcgct gatggcgctc ggcctcaacg acctgcgcga gctgttcagt tacgacatag
      661 agaacgtgcg tctacgccgc gcgcttttaa agtaatgcct gcggccgcag gattcatcga
      721 tttggagaac gcgaatgcct aagatagaag tgaacgagaa cctgttcttc gagctcgtcg
      781 gccgccgcat ggacgccgcg gagctggaac cggccctgga gaccgccaag gcggagctgg
      841 acggctggga ccgtagcggc cccgacgccg agcgcgtcat caagatagag ctcaacgaca
      901 ccaaccggcc cgacctgtgg tccaccgcgg gcgtcgcgcg ccaactacgc gtcaagctcg
      961 gcggcgccat acccgagtac ccgttcttct ccagggaggg cgaccagagg ccgacggact
     1021 atcgcgtcgt cgtcgatcag tcgatacgcg agctacgccc ctacatggcg gccttcgtca
     1081 tctccggcaa ggcgataagc gacgccatgc tgcgcgacgc catccagacc caggagaagc
     1141 tgtgctccaa cttcggccgc aagcgccgct ccgtgtcgat gggcatgtac cgcatcgagc
     1201 tgatcacatg gccggtcgcg tataagggcg tcgaccccga cgccgtcagc ttcgtaccgc
     1261 tgcagtcgga cgagagcatg accctcaggc gcatcgcggc ggagcacccg aagggcaagg
     1321 agtacggcca catcatcgcc gactcgccgg tctatcccat cctgatgggc gccgacggcg
     1381 gcgtcttatc gatggctccc atcat
//
LOCUS       DAOOLN010000637         1404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_122546_length_1404_cov_2.982209, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000637 DAOOLN010000000
VERSION     DAOOLN010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122546_length_1404_cov_2.982209"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..979
                     /locus_tag="PLW80_08320"
     CDS             200..979
                     /locus_tag="PLW80_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPB66553.1"
                     /translation="MPYSYFTIETADRVAVVTIRRPEALNALSTAVLRELDAVIGELE
                     SLAEADAVILTGEGKAFVAGADVAEMRGLSAEQGRAFGEYGSRVFRRLELLGKPVIAA
                     VNGFALGGGCELAMACDIRVASDKARFGQPEVGLGITPGFSGTQRLPRLVGVAKAKEL
                     IFTGEMIKADEALAIGLVSRVVPAEDLMGTCMDLARRIVKNAPRAVALSKAAIDRGME
                     TDLETGMALEQGFFGLCFATADQKEGMGAFVEKRSPSFGGK"
     gene            1009..>1404
                     /locus_tag="PLW80_08325"
     CDS             1009..>1404
                     /locus_tag="PLW80_08325"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HPB66554.1"
                     /translation="MKVMVLGAGTMGSGIAQAFALSGYQVIVRDLEQAFVDKGIAGVA
                     KSLDRIVAKGNLSDGDRKAALARLSGTTDLATAAEVDLVVEAAVENMAIKKAVFAELD
                     GVCKPEAILATNTSSLSITEIASATKRPDK"
BASE COUNT          259 a          406 c          497 g          242 t
ORIGIN      
        1 gcgactccgc cgccgacgtg ctcgccgagc ccgtggtcgt ctgcagggcg acgggtacct
       61 gcgtggtacc gaaggataag cagcgcgggg catgagcctc tggcgtcgtc gatatatgtt
      121 aaaaaatatc gatactaaat gcttgacaaa gcctcgggtg cggtctacat tttctggtgc
      181 cggctaagag gaggcttcga tgccgtatag ctactttacc atagagacgg ccgatcgcgt
      241 cgccgtcgta acgatacgtc ggccggaagc gctgaacgcc ttgtcgaccg ccgtgctccg
      301 ggaactggat gccgtgatcg gcgaactgga aagcctggcc gaggccgacg cggtgatctt
      361 gaccggggag ggcaaggcct tcgtcgccgg ggcggacgta gcggagatgc gcggcctatc
      421 ggccgagcag ggccgggcct tcggagagta cggctcccgc gtattcaggc gcctcgagct
      481 gctcggcaaa ccggtcatag cggccgtcaa cggcttcgcg ctcggcggag gctgcgagct
      541 cgcgatggcc tgcgatatcc gcgtggccag cgacaaggcc aggttcgggc aacccgaggt
      601 cggactcggc atcacgccgg gtttctccgg tacccaacgc ctgccccgcc tggtcggcgt
      661 ggccaaggcc aaggagctga tctttacggg agaaatgatc aaggccgacg aggcgttggc
      721 tatcggcctg gtgtccaggg tggtgcccgc cgaagatctt atgggaacgt gcatggacct
      781 ggctcggcgt atcgtcaaga acgcgccaag ggccgtggcg ctgtccaagg cggcgatcga
      841 ccgcggtatg gagaccgacc tggagaccgg catggcgctg gaacaggggt tcttcggcct
      901 gtgcttcgcg acggccgacc agaaggaagg catgggcgcc ttcgtggaga agcgaagccc
      961 gtccttcggc gggaagtagc ggtagcatcg atcctaagga gtgatgatat gaaggtgatg
     1021 gtgctgggag ccgggacgat gggttcgggg atcgctcagg cgttcgccct gtccggctat
     1081 caggtgatcg ttcgcgacct ggagcaggct ttcgtcgaca agggaatcgc cggcgtcgcc
     1141 aagagcctcg atcgcatcgt ggccaagggc aacctgtcgg acggcgacag gaaggccgcc
     1201 ctcgctcgcc tgagcggcac gaccgacctg gctacggctg ccgaggtcga cctagtcgtc
     1261 gaggcggccg ttgagaacat ggcgatcaag aaggcggtgt tcgcggagct ggatggcgtc
     1321 tgtaagccgg aggccatact cgctaccaat acctcgtcgc tgtcgattac cgagatagcc
     1381 tcggcgacca agcggcccga caag
//
LOCUS       DAOOLN010000638         1403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_122688_length_1403_cov_3.425074, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000638 DAOOLN010000000
VERSION     DAOOLN010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122688_length_1403_cov_3.425074"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..>1403)
                     /locus_tag="PLW80_08330"
     CDS             complement(75..>1403)
                     /locus_tag="PLW80_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPB66555.1"
                     /translation="GVVIGYGTVNGRLVYLFAQDFTVIGGSLGEMHAAKICKVMDMAM
                     KVGCPCIGINDSGGARIQEGVDALYGYGNIFYRNTLASGVIPQISVIMGPCAGGAVYS
                     PALTDFTVMVDKTSNMFITGPQVIKAVTGEDVSAEALGGALVHSETSGCASLMFADEK
                     ATIEGVRKLLSYLPQNNVEDAPIVSGGDDVARACPGLVGIIPDSPNKPYDIRDVIAQV
                     FDDGDYFEIQPLYAKNIVTCFARLGGRSVGVIANQPKVMAGVLDINASDKASRFIRFC
                     DSFNIPLVTLTDTAGYLPGVGQEHGGVIRHGAKLLYAYSEATVPKITVIVRKAYGGAY
                     IAMCSRHLGADQVFAWPSAEIAVMGPEGAANIIFKKDIEEATDPAAARKDKIQEYKDN
                     FANPYKAAARGYVDDVIDPSATRARLASALTMLLGKREKRPAKKHANMPL"
BASE COUNT          219 a          423 c          492 g          269 t
ORIGIN      
        1 tatgcccacg acgactatgg tccacatctg ccccgctgtc tcggtgactg ctacagtatc
       61 catagcgtcc ccttttacag cggcatgttg gcgtgcttct tggcggggcg cttctcgcgc
      121 ttgcctagga gcatcgtcag ggccgaggcg agcctagccc tcgtggcgga cgggtcgatc
      181 acgtcgtcga cgtagccgcg cgcggccgcc ttgtacggat tggcgaagtt gtccttgtat
      241 tcctggatct tgtccttgcg cgccgcggcg gggtcggtcg cctcttcgat gtccttcttg
      301 aagatgatgt tggcggcgcc ctcagggccc atcactgcga tctcggcgct cggccaggcg
      361 aagacctggt cggccccgag gtggcgcgag cacatggcga tgtacgcgcc gccgtaggcc
      421 ttgcgcacga tcacggtgat cttcggcacg gtcgcctcgg agtaggcgta caggagcttg
      481 gcgccgtggc gtatcacgcc gccgtgctcc tggccgacgc ccggcaggta gccggcggtg
      541 tcggtcagcg tgacgagggg gatattgaac gagtcgcaga agcggatgaa gcggctggcc
      601 ttgtccgaag cgttgatgtc gagcacgccg gccatcacct tgggctggtt ggctatgacg
      661 ccgaccgaac gaccgccgag tctggcgaag caggtgacga tgttcttggc gtagaggggc
      721 tggatctcga aatagtcgcc gtcgtcgaag acctgggcta tcacgtcgcg gatgtcgtag
      781 ggcttattgg gtgagtcggg gatgatgccg acgaggcccg ggcaggcgcg agcgacgtcg
      841 tcgccgccgg agacgatcgg cgcgtcttcc acgttgttct gcggtaggta cgacagcagc
      901 ttgcgcacgc cctcgatggt cgccttctcg tcggcgaaca tgaggctggc gcagccggag
      961 gtctcgctgt gcaccagggc gccgccgagg gcctcggccg aaacgtcctc gccggtcacg
     1021 gccttgatga cctgcggacc ggtaatgaac atgttggagg tcttgtcgac catcacggtg
     1081 aagtcggtca gggccgggga gtagacggcg ccgccggcgc aggggcccat gatcaccgaa
     1141 atctgcggga tcacgccgga ggccagcgtg ttgcggtaga agatgttgcc gtagccgtac
     1201 agcgcgtcga cgccttcctg gatgcgcgcg ccgcccgagt cgttgatgcc gatgcagggg
     1261 cagccgacct tcatggccat gtccatgacc ttgcagatct tggcggcgtg catctcgcct
     1321 aaactaccgc cgatgacggt gaagtcctgc gcgaacaggt agacgaggcg gccgttgacc
     1381 gtgccgtagc cgatgacgac gcc
//
LOCUS       DAOOLN010000639         1400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_123133_length_1400_cov_3.398513, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000639 DAOOLN010000000
VERSION     DAOOLN010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123133_length_1400_cov_3.398513"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /gene="yedE"
                     /locus_tag="PLW80_08335"
     CDS             complement(<1..970)
                     /gene="yedE"
                     /locus_tag="PLW80_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HPB66556.1"
                     /translation="MKTKSGFMISKAGIIVTGAVIGVLAALLVKLGNPANMGVCVACF
                     ERDIAGALGLHRAAAVQRLRPEIPAFILGSFVASLLAREWKPRTGSAPLARFVLGAFS
                     MIGALVFLGCPWRAWLRLAGGDMNAVFGLLGLFAGVLVGIVFLKRGYSLGRNHAAPAA
                     LGFVMPAIALGLLALAVVMPLFGRGADGQAIGPIFTSASGPGSMAAPIAVSLAVGLVI
                     GYLAQRSRFCTVGAVRDLVLFRDPHLMLGLLSLFAAALVTNLALGQFKLGFAGQPVAH
                     ADGLWNFGGMALSGLAFTLAGGCPGRQLFLSGEGDADAGVFVLGMIT"
     gene            complement(1043..>1400)
                     /locus_tag="PLW80_08340"
     CDS             complement(1043..>1400)
                     /locus_tag="PLW80_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPB66557.1"
                     /translation="ELADKGGVGLRLYARELRYLDGAAGLAAAGFLAGGAFRNRSFYG
                     PSVSFDSGLDEAFRGLFFVPETSGGLLAAVPCEEAEACLAELIAAGAAAAIVGVVTER
                     GQGPAMTVVDAKIDGG"
BASE COUNT          255 a          491 c          483 g          171 t
ORIGIN      
        1 cggttatcat gccgagcacg aacacgccgg cgtcggcgtc gccctctccg gataggaaga
       61 gctgccgccc ggggcatccg ccggccagcg tgaaggccaa gcccgatagc gccatgccgc
      121 cgaagttcca cagcccgtca gcgtgggcga ccggctggcc ggcgaagccg agcttgaact
      181 ggccgagcgc caggttggta acgagagccg cggcgaacag cgacaggagg ccgagcatca
      241 ggtgggggtc cctgaagagc acgagatcgc gcaccgctcc gaccgtgcag aagcgcgagc
      301 gctgggccag gtagccgatg accagcccga ccgccagcga tacggcgata ggcgcggcca
      361 tcgaaccggg gcccgaggcg ctcgtgaaga tcggaccgat cgcctgcccg tcggcgcctc
      421 ggccgaatag cggcatgact accgccaggg ccagcaggcc cagggcgata gccggcatga
      481 cgaagccgag ggcggccggc gccgcatggt tcctgccaag gctgtagccg cgcttcagga
      541 agacgatgcc gaccagcacg cccgcgaaca gaccgaggag tccgaagacg gcgttcatat
      601 cgccgccggc cagcctgagc caggcgcgcc aggggcagcc gaggaacacg agcgcgccta
      661 tcatggaaaa ggcgccgagc acgaacctgg ccagcggggc ggaaccggtc ctcggcttcc
      721 attcgcgggc caggagcgag gcgacgaagc tacccaggat gaaggccggg atctcgggcc
      781 taaggcgctg taccgcggcg gcgcgatgca ggccgagcgc cccggcgatg tcgcgctcga
      841 agcaggcgac gcagacgccc atgttcgccg gattgccgag tttgacgagc agtgcggcca
      901 ggacgcctat cacggccccg gtcactataa tgcccgcctt cgagatcatg aatccagatt
      961 tcgttttcaa ctgtatcctc cttagcgtaa gtgacgcgcg gctatccggc cgcgccccgc
     1021 ctagcgagga gcgtcggctg gatcagccgc cgtcaatttt cgcgtcgacg accgtcatcg
     1081 ccgggccttg cccccgttcg gtgacgacgc cgactatggc cgccgcggct cccgcggcga
     1141 tcagctcggc gaggcaggcc tcggcctcct cgcacggtac ggcggccagg aggccgccag
     1201 acgtctcggg cacgaagaac aggccccgga aggcctcgtc gagcccggag tcgaacgaga
     1261 cgctcggtcc gtagaacgag cggttcctga acgcgccgcc cgccaggaag ccggcggccg
     1321 ccagcccggc cgcgccgtcc aggtagcgca gctccctcgc gtacagccta agcccgacgc
     1381 cgcccttgtc ggccaattcg
//
LOCUS       DAOOLN010000640         1400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_123191_length_1400_cov_2.263197, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000640 DAOOLN010000000
VERSION     DAOOLN010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123191_length_1400_cov_2.263197"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLW80_08345"
     CDS             complement(<1..258)
                     /locus_tag="PLW80_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB66558.1"
                     /translation="MGAILVIDDEPGIRATVKDILEDEGYEALTAEDGPSGLEVLKRD
                     SVDVVVLDVWLPRMGGIDVLKAIKADHPAVETIVVSGHASID"
     gene            complement(282..>1400)
                     /locus_tag="PLW80_08350"
     CDS             complement(282..>1400)
                     /locus_tag="PLW80_08350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPB66559.1"
                     /translation="AGGASYARYFAADAGRSPDRRSRVSAIVSLRFDGEAERAASALS
                     STLAKLEVSGRIRGSSSLYIAFFSFSLVAPLLAIATLFALSVSDRLLRPVAALSDAIT
                     RIGSGARRAPYLAKPGDEAGDLVESLNALLERIERSRGDELRNEKIGAWRDIARKLAH
                     ELRNPLTPIRLSAERVLKRWKADPSSVGAVLEKSMVAIIQETANMEALLTEFRDFARL
                     PEPQKAWMELKPMVDEAVHLYSASWPELTIDCGAIDPTITLKADRGYLKQALGNLLSN
                     AAAATDGSGHVWIRADLVKTAESRYCRLQVRDDGYGIPADVRDKVFSPYFTTKPGGTG
                     LGLAIVEHIVSAHGGKIRFDSAEGAGTVFYIDLPAESQ"
BASE COUNT          231 a          457 c          449 g          263 t
ORIGIN      
        1 gtcgatggat gcgtggccgg atacgacgat ggtctccacg gccggatggt ccgccttaat
       61 cgccttgagc acgtcgatgc cgcccatcct cggtagccat acgtccagga cgacgacgtc
      121 gacgctgtct cgcttgagca cctcgagccc ggacgggccg tcctcggcag tcagggcttc
      181 gtatccctcg tcctcgagga tgtcctttac ggtggcccga atgccgggct cgtcgtcgat
      241 tacgaggatg gcgcccatgg atactcttcc ttactgctcg attattgact ctccgccggc
      301 aggtcgatgt agaaaaccgt acccgcgcct tccgcggagt cgaaacgtat cttgccgcca
      361 tgggcggaaa cgatgtgctc gacgatggcg aggcccaagc cggtcccgcc aggcttcgtg
      421 gtaaagtacg gtgaaaaaac cttgtctcgg acgtcggccg ggatgccgta cccgtcgtcc
      481 ctgacctgga gtctacagta tcgggattcc gccgttttga caagatcggc ccgtatccac
      541 acgtggcccg agccatcggt cgccgcggcg gcattggaca gcaggttgcc gagcgcttgc
      601 ttcaagtaac cccgatccgc cttcagcgta atggtcggat cgatggcgcc gcagtctatg
      661 gtcagctcgg gccacgaggc cgagtacagg tgcaccgcct cgtcgaccat gggcttgagc
      721 tccatccagg ccttctgcgg ctcgggcaat ctcgcaaaat cgcggaattc ggtcagcaac
      781 gcttccatat tggccgtttc ttgaatgatg gcgaccatgg acttctcgag caccgcgccg
      841 accgacgacg ggtccgcctt ccagcgcttg agtacccgct cggccgacag acggatcggc
      901 gtcaacgggt tgcgtagctc gtgtgccagt ttcctcgcga tatcccgcca ggcccctatc
      961 ttctcattgc gtagctcgtc gccgcggctt cgttcgatgc gctcgagcag cgcgttgagg
     1021 ctctcgacca ggtcgccggc ttcgtcgccg ggcttggcca ggtacggggc ccgcctggcc
     1081 cccgagccta tcctggttat ggcatcggag agcgccgcga ccggcctgag cagccggtcg
     1141 gatacgctga gcgcgaacag cgtggctatg gccaggagcg gtgcgaccag gctgaacgag
     1201 aagaaggcta tgtacaggct cgaggagccg cgtatgcggc cggagacctc gagcttggcc
     1261 agggtcgacg atagggccga cgcggcgcgc tcggcctcgc cgtcgaacct gagcgagact
     1321 atggccgaga cccgtgaccg ccggtctgga gagcggcccg cgtccgccgc gaagtagcgc
     1381 gcgtagctcg ccccgccggc
//
LOCUS       DAOOLN010000641         1398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_123412_length_1398_cov_3.044676, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000641 DAOOLN010000000
VERSION     DAOOLN010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123412_length_1398_cov_3.044676"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..>1398)
                     /locus_tag="PLW80_08355"
     CDS             complement(206..>1398)
                     /locus_tag="PLW80_08355"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPB66560.1"
                     /translation="PTSYDKDSISFLAWSAGGYSTLPVGLQTAAAFAPSLLTAAGLGG
                     MSATRVDEVTASLNAGLQWSIGENGEILAGKTATVDLEAFLRLVYLTSAEPGRDAMAF
                     NAARDRLASQIGPYVRDPSYRFESSWSQDLFGGNARAAPLDEAATRAVDFDETRALTV
                     SALADASEFTYVLVGDFKLDEAKALVAKHIGAIPAGSPDTPEWIAPLSPRDGGRRVDY
                     RLSREPRASVRMVWAGEAPWSWEDERTLDLLAQALNNRLLDAIREDLGGTYVVSTRAS
                     FARSPVEQYSLVIQFDTDPARVEELIAEVKAEVATLAAGSFDPRYASQVQASARRDFS
                     GRSRTNDFWANGLGNAVASGLDLGILARAEQTAGLADPDAFRALAAALLTDDSSFVYV
                     MLPE"
BASE COUNT          216 a          471 c          498 g          213 t
ORIGIN      
        1 cggcatcgtc cccggagccc cggcggtggc gcggtcgata ccgctcaaga acggcggcgg
       61 ctcggtggcc ctcgaggcgg cgccgatacg ggatgaatcg ggtagggcga cgggcttcgt
      121 catcctggtc ggcggccgtt agcctccatg caaaaagagg cctccgcgca agcggaggcc
      181 ttctcgttat tgtcgcgaca ggtacttatt cgggtagcat cacgtagacg aaggagctgt
      241 cgtcggtcag gagcgcggcc gccagggccc ggaaggcgtc cgggtcggca aggccggccg
      301 tctgctcggc tcgcgccagt atcccgaggt ctagcccgct cgccacggcg ttacccagcc
      361 cgttcgccca gaaatcgttc gtacggctcc tgccggaaaa gtcgcgcctg gccgaggcct
      421 gcacctggct cgcgtagcgg ggatcgaagc ttccggccgc cagggtggcg acctcggcct
      481 tgacctcggc tatcagctct tcgacgcggg ccgggtcggt gtcgaactgg atcaccagcg
      541 agtactgctc gaccggcgac cgggcgaacg aggcccgcgt cgacaccaca taggtgcctc
      601 cgagatcctc ccggatggca tccaggagcc ggttattgag ggcctgagcc aaaaggtcga
      661 gggtccgctc gtcctcccag gaccagggag cctctccggc ccagaccatg cgtacgctgg
      721 ccctcggctc gcgcgacagc ctgtagtcga cgcgccggcc gccgtcccgc ggcgatagcg
      781 gcgctatcca ctcgggcgta tcgggcgagc cggccggtat ggcgccgatg tgcttggcga
      841 cgagggcctt ggcttcatcg agcttgaaat cgccgacgag cacgtaggtg aactcggacg
      901 cgtcggccag cgccgagacc gtcagggccc tcgtctcgtc gaagtcgacg gctcgggtag
      961 ccgcctcgtc gagcggggcc gccctggcgt tcccgccgaa caggtcctgg ctccatgacg
     1021 actcgaagcg gtacgacggg tcgcgcacgt agggaccgat ctgggacgcg agccggtcgc
     1081 gggcggcgtt gaaggccatg gcgtcgcggc cgggctccgc cgaggtcagg tagaccaggc
     1141 gcaggaaagc ctcgaggtcc acggttgcag tcttgccagc caggatctcg ccgttctcgc
     1201 cgatggacca ctggaggccg gcgttcaacg acgcggtcac ctcgtcgacc ctggtggcgc
     1261 tcataccgcc gaggccggcc gccgtcagga gcgacggcgc gaaggccgcg gccgtctgga
     1321 gccccacggg tagcgtcgag tagccgccgg ccgaccaggc caggaagctg atcgagtcct
     1381 tatcgtagga ggtcggcc
//
LOCUS       DAOOLN010000642         1397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_123567_length_1397_cov_2.456036, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000642 DAOOLN010000000
VERSION     DAOOLN010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123567_length_1397_cov_2.456036"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PLW80_08360"
     CDS             complement(<1..830)
                     /locus_tag="PLW80_08360"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB66561.1"
                     /translation="MAKQDDFRPDWFEGELPARSFRSILKWGDPNEYKHPNRKLYALM
                     KETFGLDDEWFKKRRDEGLGVVPESSPCRLAPEHVAALTAIVGPENAHLDTYNRLRIA
                     YGKTMVDLMRLRYGVVEHLPDMALWPRDRDDLRAIVDYCEANAIPVYVYGGGSSVTRG
                     VEPFSGGVSLDMRVHLKRVIKFDERNQTISVEAGMDGPELERVLRNAPEVLGAKGRYT
                     AGHLPQSFEYSVVGGWAVTRGAGQNSTYYGKIEDIVLAQDYVCPGGVDLRTREFPAAA
                     "
     gene            complement(842..>1397)
                     /locus_tag="PLW80_08365"
     CDS             complement(842..>1397)
                     /locus_tag="PLW80_08365"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPB66562.1"
                     /translation="LDELLRSTPPGGHGLVMQPYWTAGLSSPSAKGAIIGFGDVHERS
                     HLYRAIVEGLAYGLKEGLEAIERASGTRVRSLRVSGGASQSDAICQASADVLDLPMER
                     GRTCEASGLGAAMCAAAGLGWYPGVEAASAAMSSSSRVFEPDGVNAELYAELYRRVYK
                     RMYRALGPLYESIRDITGYPERAV"
BASE COUNT          202 a          500 c          469 g          226 t
ORIGIN      
        1 tcggccgcgg ccgggaactc gcgggtgcgc aggtcgacgc cgccggggca gacgtagtcc
       61 tgggccagca cgatatcctc gatcttgccg tagtaggtcg agttctgccc cgctccgcgc
      121 gtgacggccc agccgccgac caccgagtac tcgaagctct gcggcaggtg gccggccgtg
      181 tagcggccct ttgccccgag cacctcgggc gcgttcctga gcacgcgctc cagctcgggg
      241 ccgtccatgc cggcctcgac gctgatcgtc tggttccgtt cgtcgaactt gatcacgcgc
      301 ttcaggtgca cgcgcatgtc caggctcacg ccgcccgaga acggctcgac gccgcgcgtc
      361 actgaggagc cgccgccgta gacgtagacc ggaatagcgt tggcctcgca gtagtcgact
      421 atggcgcgca ggtcgtcgcg gtcgcgcggc catagcgcca tgtccggcag gtgctcgacg
      481 acgccgtagc gcaggcgcat caggtcgacc atcgtcttgc cgtaggctat ccgcagccgg
      541 ttataggtat cgagatgggc gttctcggga cccacgatgg cggtcagcgc cgcgacgtgc
      601 tcaggggcca ggcggcaggg cgacgactcg ggtacgacgc cgaggccctc gtcgcgccgc
      661 ttcttgaacc actcgtcgtc gaggccgaag gtctccttca tcagcgcgta cagcttgcgg
      721 ttcgggtgct tgtactcgtt cgggtcgccc cacttgagga tggaccggaa actgcgcgcg
      781 ggcagttcgc cctcgaacca atccggcctg aagtcgtctt gcttagccac atcgtcctcc
      841 tttagacggc ccgctcgggg tatccggtaa tgtccctgat gctctcgtac aggggcccga
      901 gggcccgata catgcgcttg tagacgcggc ggtacagctc ggcgtatagc tccgcgttga
      961 ccccgtcagg ctcgaagacg cgggacgacg acgacatggc cgcgctcgcc gcctccacgc
     1021 ccggatacca gcccagcccg gcggcggcgc acatcgccgc cccgaggccc gaggcctcgc
     1081 aggtgcgccc ccgctccatc ggcaggtcga gcacgtcggc cgaggcttgg cagatcgcgt
     1141 cgctctggct ggcgccgccc gagacccgca gcgagcgcac cctcgtcccg gacgcccgct
     1201 ctatcgcctc gaggccctcc ttgaggccgt aggccaggcc ctcgacgatg gcgcggtaca
     1261 ggtgcgatcg ctcgtgcacg tcgccgaagc ctatgatagc gcccttcgcc gacggcgacg
     1321 acaggccggc cgtccagtac ggctgcatca cgaggccgtg gccgcccggc ggggtcgagc
     1381 gcagcagctc gtcgagc
//
LOCUS       DAOOLN010000643         1394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_123991_length_1394_cov_3.074683, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000643 DAOOLN010000000
VERSION     DAOOLN010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123991_length_1394_cov_3.074683"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PLW80_08370"
     CDS             complement(<1..555)
                     /locus_tag="PLW80_08370"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB66563.1"
                     /translation="MAECVPLRTKIAFGVGDVYGGGSFNIINFLYAFYLANVLGIPTA
                     WAAAIMMVARLWDAVSDPLMGFITDNTRTRLGRRKPYFIAGIPLIVVSMAWVWYPLAT
                     ESMALRVLFAASGYIFYNTVTTLVMVPYMSMAAEISLDYYERNSINTVRMMFSLGSSL
                     MCALLPLAIVEYVSGRTGSYASGYL"
     gene            880..>1394
                     /locus_tag="PLW80_08375"
     CDS             880..>1394
                     /locus_tag="PLW80_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPB66564.1"
                     /translation="MKRFIYVAIAAFVLSAAGCVSTPKASAVPAPVASAAAKDSPISM
                     AQLDEYLFRSDVMVVDLRNFEERFNSGYIAGTESLPFFQFLEGRMVTRGLVDGKPTSW
                     DITKAEINDGFVYSNYFKPGKTVILFCASGTRAAFVKSILDAKGYTTFNLGAFKDYKG
                     VNKVLGNLLSDP"
BASE COUNT          289 a          425 c          393 g          287 t
ORIGIN      
        1 cagataaccg gaggcgtagc tgccggtacg gcccgacacg tactcgacga tggcgagcgg
       61 cagcagcgcg cacatgagcg acgagccgag agagaacatc atacggacgg tattgatgga
      121 gttacgctcg tagtagtcga gcgatatctc ggcggccatc gacatgtacg gcaccatcac
      181 gagcgtcgtc accgtgttgt agaagatata gccagacgcc gcgaatagta cgcgcaacgc
      241 catcgactcc gtggccaggg gataccatac ccatgccatc gacacgacga tgagcgggat
      301 gcccgcgatg aagtacggct tgcggcgccc gagccgcgtc ctcgtgttgt cggttatgaa
      361 gcccatcagc gggtcgctga ccgcgtccca tagccgcgcg accatcatga tggcggccgc
      421 ccacgccgtc ggtataccga ggacgttggc caggtagaag gcgtacagga agttgatgat
      481 attgaaggag ccgcctccgt agacgtcgcc gactccgaag gctatcttgg tccgtaaggg
      541 tacgcactca gccatggtcc ctccatagtg cgttctatcc ttcagtctat ctcgagcctg
      601 ccgacctcga acatacgcga gtctacgcta tcctcgttct ccctggatag gtccgagacg
      661 cggcccgcgg ctatggctat ctgctcggcg ctcggacgat cggcagtcct gtttatagta
      721 tttattctat cttgactttt ttatatattt cgctaatcat atcgccttgg agctactcaa
      781 tatcgagacg atcacggtag ccgggcacct cgccgttgcg ctcaggatgc ccatgcgcga
      841 tacttgatcg tctagggtac atcccacaag gagaaggaca tgaagagatt catctatgtc
      901 gcgatcgcag ccttcgttct ttcagccgca ggttgcgttt caaccccaaa ggccagcgcc
      961 gttccggcac cagtggcgtc cgccgctgcc aaggactcgc ccatatcgat ggcgcagctc
     1021 gacgagtacc tgttccgttc cgacgtcatg gtcgtcgacc tgcggaactt cgaggaacgc
     1081 ttcaacagcg ggtacatcgc cggcaccgag tcgcttccgt tcttccagtt cctggagggc
     1141 cgcatggtga cccgcgggct cgtggacggc aagccgacgt cctgggatat aaccaaggcg
     1201 gagatcaacg acggcttcgt ctattcgaac tacttcaagc ccggcaagac cgtcatcctc
     1261 ttctgcgcgt ccggtacgcg agccgctttc gtaaagagca tcctcgacgc caagggctat
     1321 acgaccttca acctcggcgc cttcaaggat tataagggcg tcaacaaggt gctcggcaac
     1381 ctgctcagcg atcc
//
LOCUS       DAOOLN010000644         1393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_124111_length_1393_cov_3.890882, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000644 DAOOLN010000000
VERSION     DAOOLN010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124111_length_1393_cov_3.890882"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PLW80_08380"
     CDS             complement(<1..359)
                     /locus_tag="PLW80_08380"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB66565.1"
                     /translation="MDLGLSGRTALVAASTDGLGLATARVLVAEGANVWIGGRDPARL
                     AAALESLRAPERDGRRVGRVEGAVLDVTDAASIKAWVAGAAVAYGPAIDALTVNAGGP
                     PPGLFIDLDDDAWRKAF"
     gene            540..1040
                     /locus_tag="PLW80_08385"
     CDS             540..1040
                     /locus_tag="PLW80_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66566.1"
                     /translation="MHTKQTETVMIVGLLILALSAPAYAQGSFKEGKALSLGSEGGVF
                     SKPTYAQEGRLVAAGAVDAFLLYDLEETRTVMLEVARLDTAHVATSIGGTALMIGGLA
                     TFFVAASGVSDNRGTISIYLTSAGLFLGSAGAWAGNWHCQKQRVRLRLEAIELYNRRY
                     GLVAEP"
     gene            complement(1068..>1393)
                     /locus_tag="PLW80_08390"
     CDS             complement(1068..>1393)
                     /locus_tag="PLW80_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66567.1"
                     /translation="GTKAGTKAGTKAGSASDLSIKASGDGSAYDLVYGGKWKLFTGDE
                     MLAMVRIATVKDPQDQVAGRLYRWLLAERSDVINDLGLSGLADPMLKHLVALLRTTFT
                     IKPGR"
BASE COUNT          239 a          477 c          445 g          232 t
ORIGIN      
        1 tcgaaggcct tgcgccacgc gtcgtcgtcc aggtcgatga acaggcccgg aggagggccg
       61 cccgcgttca cggtcagcgc gtcgatagcc gggccgtagg ccacggcggc accggcgacc
      121 caggccttga tcgaggcggc gtccgtaacg tcgagtacgg cgccttcgac ccggccgact
      181 cgtcgtccgt cacgttcagg cgcgcgcaaa gactcgagcg cggccgccag cctagccggg
      241 tcacgcccgc ctatccatac gttcgcgccc tcggctacca gcaccctggc cgtggcgagc
      301 cccagcccat cggtagacgc cgcgacgagg gccgttctgc cgctcagtcc tagatccata
      361 tccacctccc agactatcgg atctaaagat acgccgtctg ccaccggcga tcaaatcggt
      421 tgatgcccgc gcggaccttg cgcccccgga caggcgggct atacttgatc gtatacggta
      481 gaaagtatcc gcatcgtgtc gttcaacgct accatagatc gaagcagtgg aggtaagcga
      541 tgcacacgaa gcaaacggag accgtcatga tcgtcggcct cttgatcctc gcgctctccg
      601 ccccggccta cgctcaaggc agcttcaagg aaggcaaggc gctgagccta ggctccgagg
      661 gcggcgtctt ctcgaagccg acctacgctc aggaaggcag gctcgtcgcg gctggagccg
      721 tcgacgcgtt cctgctctac gacctcgagg agacgaggac cgtcatgctg gaggtcgcgc
      781 gcctcgatac ggcccacgtc gccacctcga taggcggcac cgccctgatg ataggaggcc
      841 tggccacctt cttcgtcgcc gcgagcgggg tctcggacaa tcgcggcacc atcagcatct
      901 acctgacctc ggccggcctg ttcctcggca gcgccggagc ctgggcgggc aactggcact
      961 gccagaaaca gcgcgtacgc ctgcgactgg aggctatcga gctctacaac cggcgctacg
     1021 gcctggtagc ggagccctga gatgcgccgt tcgattggac cggctcccta gcgaccgggc
     1081 ttgatcgtga aggtcgtgcg aagtagcgcg acgagatgct tgagcatcgg atccgccagg
     1141 cccgacaggc ccaggtcgtt gatcacgtcg gatcgctcgg ccaggagcca gcggtagagc
     1201 ctgccggcca cctggtcctg cgggtccttg accgtggcga tgcggaccat cgcgagcatc
     1261 tcgtcgccgg tgaacagctt ccacttgccg ccatagacca ggtcgtaggc cgagccgtct
     1321 cccgaggcct tgatcgacag gtcggaggcg gagccggcct tggttccggc cttggttccg
     1381 gccttggttc cga
//
LOCUS       DAOOLN010000645         1393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_124189_length_1393_cov_2.237668, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000645 DAOOLN010000000
VERSION     DAOOLN010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124189_length_1393_cov_2.237668"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLW80_08395"
     CDS             complement(<1..185)
                     /locus_tag="PLW80_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66568.1"
                     /translation="MEFAILILASLGITNAITREYVFEWFRDWLERMLGDCAVTKLFG
                     CETCMGFWVGLALAPFAP"
     gene            complement(185..769)
                     /locus_tag="PLW80_08400"
     CDS             complement(185..769)
                     /locus_tag="PLW80_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66569.1"
                     /translation="MATNTVYFISEQYVKDNSPVSLAADAKLISVSILDAMEVYVQPA
                     LGTRLYAKLKSLIAAGTITDPANAEYKDLLDNYVSRAVLYHAVSELTTFVRWKITAKS
                     VDGQSSDNGAPADLEELKYFRGELRNKAEFYTQRLIDRLAERGSTHLPEYGEATNEDE
                     IDPRDNAYRTGMALDDDCPCLRKMGYNDGIRRLD"
     gene            complement(772..>1393)
                     /locus_tag="PLW80_08405"
     CDS             complement(772..>1393)
                     /locus_tag="PLW80_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66570.1"
                     /translation="IETDAEIANFALASIRNGMAPSILINFPNGLPSPEEERAIEKKI
                     RQKYQGSDNAGKFILTFSEGADKAPTVTTIAATDLDKQFIQLKETVMQGILSGHGVVS
                     PLLVGIPSPVGLGGTSIVKEAWQLYESRVVLPLRKKVERAVSDVMRFNGWRPVEIMSS
                     SPVEFSYSEGVLQNVLTVDEMRENIGYPPKAPQNSPDAIVDENKQQ"
BASE COUNT          236 a          438 c          394 g          325 t
ORIGIN      
        1 ggggcgaacg gcgcgagcgc gagccccacc cagaacccca tacacgtctc gcagccgaac
       61 aacttggtta ccgcgcagtc gccgagcatc ctttcgagcc agtccctgaa ccattcaaag
      121 acgtactcgc gcgtaatcgc gttggttatg cccaatgagg cgagtatgag tattgcgaat
      181 tccatcagtc aagcctcctt atcccgtcgt tgtagcccat tttcctaagg caggggcagt
      241 cgtcgtcgag agccataccc gtgcggtacg cgttgtccct ggggtcaatc tcgtcctcat
      301 tggtcgcctc gccgtactcc ggcaggtgcg tcgatccgcg ctcggcgagc ctgtcgatca
      361 gccgctgcgt gtagaactcg gccttgttgc gcagctcgcc cctgaagtac ttgagctcct
      421 cgaggtcggc gggcgcgccg ttgtcagaag actggccgtc cacgctcttc gccgttatct
      481 tccacctgac gaacgtggtg agctccgaca ccgcgtggta cagcaccgcg cgcgacacgt
      541 agttgtcgag caggtccttg tattcggcgt tggccgggtc tgttatcgtg ccggccgcta
      601 tcagcgactt gagcttcgcg tagagcctgg tgccgagcgc cggctggacg tacacctcca
      661 tcgcgtcgag tatcgacacg cttataagct tggcgtccgc cgccagcgag acgggcgagt
      721 tgtcctttac gtactgctcg cttatgaagt aaaccgtgtt ggtagccata tttactgctg
      781 tttattttcg tccacgatcg cgtcaggcga gttctgggga gctttcggcg ggtagcctat
      841 gttttccctc atctcgtcca ccgtaagcac gttctggagc accccttcgg agtaagagaa
      901 ctccaccggg ctcgaagaca ttatctctac cggcctccag ccgttgaacc gcatcacgtc
      961 gctaaccgcg cgctcgacct tcttcctgag cggaagcacc accctcgact cgtaaagctg
     1021 ccacgcctcc ttcactatgc tcgtgccgcc gagccccacc ggcgaaggta tgccgacgag
     1081 gagcggagac actactccgt gccctgaaag tattccctgc ataacggtct ctttgagctg
     1141 gatgaactgc ttgtcgaggt cggtcgccgc tatcgtcgta actgtaggag ccttgtcggc
     1201 gccttcggag aacgtgagta tgaacttgcc ggcgttgtcg ctgccctggt acttctgcct
     1261 tatcttcttc tcgatcgcgc gctcttcctc cggcgagggg aggccgttgg ggaagtttat
     1321 caatatgctc ggggccatac cgttgcgtat gctggcgagc gcgaagttcg ctatctcggc
     1381 gtccgtctct atc
//
LOCUS       DAOOLN010000646         1392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_124332_length_1392_cov_2.096485, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000646 DAOOLN010000000
VERSION     DAOOLN010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124332_length_1392_cov_2.096485"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PLW80_08410"
     CDS             <1..473
                     /locus_tag="PLW80_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HPB66571.1"
                     /translation="INIAPDLQGKLDIIANAVAVSRSIGVELPKVAMLAAVEKVNPEK
                     MPCTADAALITQMNRRGQVKGCVVDGPLALDNAISAESARIKKIVSDVAGDADVLVVP
                     DIEAGNVLYKALLDLGGAKGAGIVAGAAKPIVLTSRADSRETKLASIALAALAG"
     gene            640..774
                     /locus_tag="PLW80_08415"
     CDS             640..774
                     /locus_tag="PLW80_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB66572.1"
                     /translation="MLAADTEPNSWGYYPSTAVAPEKCIACGNCYAVCPDVAISVYKL
                     "
     gene            787..>1392
                     /locus_tag="PLW80_08420"
     CDS             787..>1392
                     /locus_tag="PLW80_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HPB66573.1"
                     /translation="MAAEKRLMKGNEAMGEAAVRAGCDAYFGYPITPQNELTAYMAKH
                     MIDHKRVFIQAESEVSAINMVYGAAATGARAMTSSSSPGVSLKQEGISYACGADVPMV
                     IVNVARSGPGLGGISPAQGDYFQSTRGGGHGDYHHIVLAPKSVQEAADLTFEAFDLAD
                     EWRMPAMILADGMIGQMMEGVVLPEPKDPATRVAKPWAVGNK"
BASE COUNT          269 a          446 c          478 g          199 t
ORIGIN      
        1 cgatcaatat agcgcccgac ctgcagggta agctcgacat catcgcgaac gcggtggccg
       61 tgtcccgctc gatcggcgtg gagctaccga aggtggccat gctggcggcg gtggagaagg
      121 tcaacccgga gaaaatgccg tgcacggccg acgccgccct gatcacgcag atgaaccggc
      181 gcggacaggt gaagggctgc gtcgtcgacg ggccgctggc gctggacaac gcgatcagcg
      241 ccgagagcgc ccgcatcaag aagatcgtct ccgacgtggc cggcgacgct gacgtattgg
      301 tcgtgccgga catcgaggcg ggcaacgtgc tgtacaaggc gttgctggac ctgggcggcg
      361 ctaagggggc gggaatcgtc gccggggcgg ccaagccgat cgtgctgacg agccgggccg
      421 acagccggga gacgaagctg gcgagcatag ccctggcggc gctcgccggc tagccgcgag
      481 gaacgcattt ggcaatcgcc gggggccgcg cggtccccga taccattcta cgcgtacggg
      541 aagtacgaca aggagtcgcc atggctacta aaggaaaacc cgagatcgat cgggaacgat
      601 gcaagggctg cctgctctgc gtccgcctgc ccggtcaagg tgctcgcggc cgatacggag
      661 cccaattcgt ggggatatta tccatccacg gccgtagcgc ccgagaagtg catagcctgc
      721 ggtaattgct acgcggtctg cccggacgtg gcgatcagcg tctataagct ataaggaggg
      781 tccggcatgg ctgcagagaa gcgcctgatg aaaggcaacg aggctatggg cgaggccgcc
      841 gtccgcgctg gatgcgacgc gtacttcggc taccccatca ccccgcagaa cgaactgacc
      901 gcgtacatgg ccaagcacat gatcgaccac aaacgcgtgt tcatacaggc cgagagcgag
      961 gtctccgcga tcaacatggt ctacggggcc gccgcgaccg gcgcccgcgc aatgaccagc
     1021 tcgtcgagcc ccggcgtgtc gctcaagcag gagggcatct cctacgcctg cggcgccgat
     1081 gtaccgatgg tcatcgtcaa cgtggcgcgc tccggccccg gcctcggcgg catctcgccg
     1141 gcccagggcg actacttcca gtcgacccgc ggtggcggcc acggagacta ccaccacatc
     1201 gtgctggcgc ccaagagcgt ccaggaggcg gccgacctga ccttcgaggc cttcgacctg
     1261 gccgacgagt ggcgcatgcc cgcgatgatc ctggccgacg gcatgatagg ccagatgatg
     1321 gaaggcgtcg tcctgcccga gccaaaggat ccggctacgc gcgtcgcgaa gccctgggcc
     1381 gtcggcaaca ag
//
LOCUS       DAOOLN010000647         1391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_124436_length_1391_cov_2.941617, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000647 DAOOLN010000000
VERSION     DAOOLN010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124436_length_1391_cov_2.941617"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1391)
                     /locus_tag="PLW80_08425"
     CDS             complement(<1..>1391)
                     /locus_tag="PLW80_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HPB66574.1"
                     /translation="MDGTKAKTLSLSIDGRELSVCEGTTILEACRGAGVRIPTLCHLP
                     GVSSNASCGLCVVEVEGAKSLVRSCVQAAAPGMRVRTASARVMEARRTVVELLLANHP
                     DDCLSCLRNGGCELQAAAELLGVRAKAFPRTKKQAKPDKAGWGVLRDNDKCILCGRCI
                     AVCRETQSVMAIDFAGRGVNSRVAPFLDSPLSESVCVSCGQCTVVCPTGALTERDETD
                     EVFAAINDPELTVVVQTAPAIRASLGEALGMQAGSLVTGKMAAALRRLGFDKVFDTQF
                     SADLTIMEEGSELLRRIGERGTLPMITSCSPGWISFIETFYPALLPHVSTCKSPQQMF
                     GSIAKTFWARKAGVDPSKLVVVSIMPCTAKKYEAKRPEMRSAWEWWKDHGGKDSPYFD
                     VDYALTTRELARMVRRAGIDFEALPDESFDDPLGASTGAATIFGSTGGVMEAALRTAY
                     ELAVGEPLPSLDL"
BASE COUNT          217 a          467 c          474 g          233 t
ORIGIN      
        1 cgagatccag gctcggcagg ggctcgccga cggccagctc gtacgcggtg cgcagcgcgg
       61 cctccatcac gccgccggtc gagccgaaga tggtcgcggc cccggtgctg gcgccgagcg
      121 gatcgtcgaa gctctcgtcg ggcagggcct cgaagtcgat gcccgcccgg cgtaccatgc
      181 gggccagctc cctcgtcgtc agggcgtagt cgacgtcgaa gtacggcgag tccttccccc
      241 cgtggtcttt ccaccattcc caggcgctac gcatctccgg ccgcttggcc tcgtacttct
      301 tggcggtgca tggcatgatc gacacgacga ccagcttgga cgggtcgacg ccggctttcc
      361 gggcccagaa ggtcttggct atcgaaccga acatttgctg gggcgacttg caggtggaga
      421 catggggcag gagcgctggg tagaaggttt cgatgaagct gatccagccc ggcgagcacg
      481 aggtgatcat cggtagcgtc ccacgctcgc cgatacgcct gagcagctcc gaaccttcct
      541 ccatgatggt caggtcggcc gagaactgcg tgtcgaagac cttgtcgaag ccgagcctcc
      601 ggagcgcggc cgccatcttg ccggtgacaa ggctgcccgc ctgcatgccg agcgcctcac
      661 cgagcgatgc gcgtatcgcc ggcgcggtct ggaccaccac ggtcagctcg gggtcgttga
      721 tggcggcgaa cacctcgtcg gtctcgtcgc gctccgtcag ggccccggtc gggcagacga
      781 cggtgcattg accgcaggag acgcagaccg actcggacag ggggctgtcc aggaacggcg
      841 ccaccctgga gttgacgccg cggccggcga agtctatggc catgaccgac tgggtctcgc
      901 ggcagacggc gatgcagcgg ccgcagagta tgcacttgtc gttatcgcgt aggacgcccc
      961 agcctgcctt gtccggcttg gcctgcttct tggtccgcgg gaaggccttg gcacgcacgc
     1021 cgagcaactc cgctgccgcc tgcagctcgc agccgccgtt ccggaggcac gagaggcaat
     1081 cgtcgggatg attggccagg agcagctcga cgacggtacg ccgggcctcc atcacgcgag
     1141 cgctggccgt gcgtaccctc atgcccggcg cagccgcctg cacgcaggag cgcacgaggc
     1201 tcttggcgcc ctcgacctcg acgacgcaga ggccgcatga ggcgttcgag ctgacgcccg
     1261 gcaggtggca gagggtcggt atcctgacgc cagcaccgcg gcaggcttcg agtatcgtcg
     1321 tcccttcgca tacgctcagc tcgcggccgt cgatcgatag gctaagggtc ttggctttgg
     1381 ttccatccat g
//
LOCUS       DAOOLN010000648         1391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_124437_length_1391_cov_2.889222, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000648 DAOOLN010000000
VERSION     DAOOLN010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124437_length_1391_cov_2.889222"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..1157)
                     /locus_tag="PLW80_08430"
     CDS             complement(69..1157)
                     /locus_tag="PLW80_08430"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPB66575.1"
                     /translation="MEEKITVKEIARLAGVSIGTVDRVLHDRGGVSSKTQARIREIIS
                     SLGYEPDVLARQLSLKKNFTFRVILPRSDQDSGYWALCLQGIRRAELDLARYRCDVRV
                     DEFDRYDEAAYRVLLESVLEDPCDGLLLAPAMPEILCGALARLGSRAGGDGTRDEPGS
                     TPYVFFDGILECASPLAVVAQDPFRGGYLAGRVMSLLSPGAGRLVALSAHAEDRHIAR
                     RIDGFRSFFSDHHSERRIDAVDCPGLDGSSAKEGALERIFGDAADISGILVANSSGHL
                     VGDWLAARGLKTKLSLVSWDAVPDNVRALADGSIDCLVSQRPAEQAREALELLFHAVV
                     LGEGALASRRPAEIPLELFFRENIPLGE"
     gene            complement(1166..>1391)
                     /locus_tag="PLW80_08435"
     CDS             complement(1166..>1391)
                     /locus_tag="PLW80_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608103.1"
                     /note="membrane component of a putative sugar ABC
                     transporter system; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HPB66576.1"
                     /translation="GVGNLIGSPIGALTLLTINELIRAAGVNSNFQSIVSGLLLCIFI
                     VLQSVVLWLRGARGLRRALPAWLTPSGKRG"
BASE COUNT          227 a          450 c          481 g          233 t
ORIGIN      
        1 tttgttagtc atctcgagcc tcctcgcggt cccggtcgcc ggaagggcgg cggcccggat
       61 cgcgtattct attctcccag cgggatattc tctctaaaga acagttcgag cggtatctcc
      121 gccggacggc gcgacgccag cgccccttca ccgagcacca ccgcgtggaa gagaagctcg
      181 agcgcctcgc gcgcttgctc ggccgggcgc tgcgacacga ggcagtcgat cgagccgtcg
      241 gcgagggccc gcacgttatc cggtacggcg tcccatgaga cgagcgatag cttggttttg
      301 agccccctgg ccgcgagcca gtccccgacg aggtggcccg aggaattggc cacgaggatg
      361 cccgagatgt cggcggcgtc cccgaatatc ctctcgagcg ctccctcctt ggccgagcta
      421 ccgtcgagtc cggggcaatc gacggcgtcg atgcgccgtt ccgagtggtg gtcgctgaag
      481 aaggatcgga acccgtcgat gcgccgggcg atatgcctat cctcggcatg cgccgagagg
      541 gctacgagcc ggccggcgcc cggcgacagg agcgacatga cgcgcccggc taggtagccg
      601 cctcggaacg ggtcctgggc cacgacggcg agcggcgagg cgcactcgag gatgccgtcg
      661 aagaagacgt agggcgtcga gccgggctcg tcccgggttc catcgccccc ggcgcggctt
      721 ccgagcctgg ccagggctcc gcagagtatc tccggcatgg cgggagcgag gaggaggccg
      781 tcgcagggat cctcgaggac gctctcgagc aatacccggt aggccgcctc gtcgtagcgg
      841 tcgaactcgt cgacgcgcac gtcgcagcgg tatcgggcga ggtcgagctc ggctcgcctg
      901 atgccctgga ggcagagcgc ccagtaaccc gaatcctgat ccgatcgcgg caggatcacg
      961 cggaaggtga agttcttctt gagcgatagc tgcctcgcga ggacgtcggg ctcgtagccg
     1021 agcgacgata tgatctcgcg tatcctcgcc tgcgtcttgg aggagacgcc gccccggtcg
     1081 tggagtacgc ggtcgacggt gcctatggac accccagcga ggcgggctat ctcctttacg
     1141 gtgatctttt cttccatgtc tctatctatc cgcgcttgcc gcttggcgtc agccatgcgg
     1201 gtagggcgcg ccgtaggccg cgcgctccgc gtagccagag gacgacgctc tgcagcacga
     1261 tgaagatgca caggagcagc ccgctcacga tggactggaa attcgagttg acgccggcgg
     1321 cgcgtatgag ctcgttgatc gtgagcaggg tcagcgcccc gatgggcgag cctatcaggt
     1381 tcccgacgcc g
//
LOCUS       DAOOLN010000649         1390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_124574_length_1390_cov_3.007491, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000649 DAOOLN010000000
VERSION     DAOOLN010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124574_length_1390_cov_3.007491"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1390
                     /locus_tag="PLW80_08440"
     CDS             <1..>1390
                     /locus_tag="PLW80_08440"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPB66577.1"
                     /translation="PAGPGDVAAEREIVVAVRDPSDAGLQQRGKQALKPAGILYTATS
                     GIWQTVWLEPLPAGNAILGFRADATAGLDGVTAVVDAERPAEVVLRASLPDGGVIEAR
                     GRSGEPIAVSVPDVRRWSPDDPYLYGVEVELLGPDGDIVDRADSYFAIRRVDLGSAHG
                     SPSGVPSGSPSGSRADERPFVRLNGEPILIHAPLDQGYWPESGMTPPADEALVFDIEA
                     MRALGFNGLRKHVKVESRRFYWHCDRLGTLVIQDAVSGGVNRSAGNVKVALPMIFGIH
                     LGDRSGRALRLAGRSDPDNRAEFESDLAGMMDLLRCHPSVVMWTVFNESWGQFESERV
                     VGAVRRRDPTRLVDAVSGWHDQGGGDFRSRHTYLVKLRRPPRRDRRPYFVSEYGGYNL
                     AVPGHMWDDEARFGYRFYDDGPALQEGYARLIRDELIPLVRSGLRVAVYTQVSDVEIE
                     SNDFLYEIENGLS"
BASE COUNT          203 a          520 c          455 g          212 t
ORIGIN      
        1 cccgccggac cgggcgacgt cgccgcggag cgcgagatag tcgtcgcggt gcgcgacccg
       61 agcgacgcgg ggctacagca gcgcggcaag caggcgctca agcccgccgg catactctat
      121 acggcgacca gcggcatctg gcagaccgtc tggctcgagc cgctccccgc gggcaacgcc
      181 atactcggct tccgcgccga tgctacggcg ggcctcgacg gcgtcacggc cgtcgtagac
      241 gcggagcggc cggccgaggt cgttctccgc gcgtccctgc cggacggcgg cgtcatcgag
      301 gcgcgcggcc gatccggcga acctatcgcc gtatccgtcc cggacgtgcg ccgctggtct
      361 cccgacgatc cgtacctgta cggcgtggag gtcgagctgc tcgggccgga cggcgatata
      421 gtcgaccgcg ccgactcgta cttcgcgata cgccgcgtcg acctcggctc ggcgcacggc
      481 tccccttcgg gcgtcccttc gggctcccct tcgggatcgc gagccgacga gcgccccttc
      541 gtgcgcctga acggcgagcc catcctgatc cacgcgccgc tcgaccaggg ctactggccg
      601 gagtcgggca tgaccccgcc ggccgacgag gcactcgtct tcgatatcga ggccatgcgg
      661 gcgctcggct tcaacggcct ccgcaagcac gtcaaggtcg agtcgcgccg cttctactgg
      721 cactgcgacc gcctcggcac gctcgtcatc caggacgccg tatccggggg cgtcaaccga
      781 tcggccggga acgtcaaggt ggccctgccg atgatattcg ggatccacct cggcgaccga
      841 tccggccgcg ccctgcggct cgctggccgc tccgaccccg ataaccgtgc cgagttcgaa
      901 tccgacctgg ccggcatgat ggacctacta cgctgccacc catcagtcgt catgtggacc
      961 gtcttcaacg agtcatgggg ccagttcgag tctgagcgcg tcgtcggggc cgtacggcgc
     1021 cgggacccga cccgcctcgt cgacgccgtc tccggctggc acgaccaggg cggcggcgac
     1081 ttccgtagcc gccataccta cctggtcaag ctgcgccgac cgccgaggcg cgaccggcgg
     1141 ccgtacttcg tctccgagta cggcggctac aacctcgccg tgcccggcca catgtgggac
     1201 gacgaggcgc gcttcggcta ccgcttctac gacgacggcc ccgcgctcca ggagggctac
     1261 gcgcgcctga tccgcgacga gctcataccg ctcgtccgta gcggcctccg cgtcgcggtc
     1321 tacacccagg tctccgacgt cgagatcgag agcaacgatt tcctgtacga gatagagaac
     1381 ggattgtcct
//
LOCUS       DAOOLN010000650         1389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_124718_length_1389_cov_2.574213, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000650 DAOOLN010000000
VERSION     DAOOLN010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124718_length_1389_cov_2.574213"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..>1389)
                     /locus_tag="PLW80_08445"
     CDS             complement(115..>1389)
                     /locus_tag="PLW80_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPB66578.1"
                     /translation="MLVTTGLIAAKPVQAWIMTLLNLLAIGALYYLDALPLDGGVVTE
                     LAMQNLGTCGVIVIAAGIFSSMAIKMQSELVDDTRRSADAARIQFERMAAAVSDAQAG
                     ALEIGTRLAGAADTVSSTAASMRDVSSEELSGLASLIDALAAAEDGEKVADAAQDRVS
                     SSLEAYSAKVLEASASIAQMVEAVDGIGKAASERLEVVAKLVDMVKAGDERVTRIGEA
                     IADIVSATSKMDEMNGLIGQVAERTNMLGMNAAIEAAHAGDAGKGFAVVAEEIQSLSD
                     TAAEGSGSIASILSETAEVVAGATKASAQTSEFFSRLSEEIQAMSSTFGDLLMKLQEV
                     SAGTAGVTEAIRGFSELSVAAGAAVDETREAFRSTVARAASSREVASAMRQAMDRMAA
                     SCDELLAQAASLSELGQENVRKMEGLRDKLGA"
BASE COUNT          212 a          475 c          444 g          258 t
ORIGIN      
        1 agggtgagta acgcgcgggc cgatagggcc atcgcggctt ttatgctgta gcctcgtagc
       61 atcgttcctc cgggtttcac ggctcgaagc ggccgctcat cggcctttcg ggccctaggc
      121 ccccaacttg tcgcggaggc cttccatctt gcgcacgttc tcctgaccga gctcgctcag
      181 cgaggcggcc tgggccagta gctcgtcgca ggaggcggcc atcctatcca ttgcctgacg
      241 catcgccgag gcgacctcgc gggacgaggc ggcgcgggcc acggtcgacc tgaacgcctc
      301 cctggtctcg tctactgcgg cgccagcggc tacagatagc tccgaaaagc ccctgatcgc
      361 ctcggtaacc ccggccgtgc cagccgatac ttcctggagc ttcatcagaa ggtctccgaa
      421 ggtcgacgac atcgcctgta tctcctcgga cagcctggag aagaactcgc tggtctgggc
      481 gctggccttc gtcgcgccgg cgaccacctc ggcggtctcg gacagtatcg aggctatcga
      541 tcccgagccc tccgccgcag tatccgagag gctctggatc tcctcggcca ccaccgcgaa
      601 acccttgccg gcgtcgcccg catgggcggc ctctatggcc gcgttcatcc cgagcatgtt
      661 cgtgcgctcg gcgacttggc cgatcaagcc gttcatctcg tccatctttg aggtggccga
      721 cacgatgtcg gctatagcct cacctatcct ggtcacgcgt tcgtcgccgg ccttgaccat
      781 gtcgacgagt ttcgcgacga cctcgagccg ctccgaggcg gccttgccta tgccgtccac
      841 cgcctcgacc atctgagcta tcgaagcgga tgcctcgagt accttggccg agtaggcctc
      901 gagcgacgag ctgactcggt cctgggccgc gtcggccacc ttttctccgt cctcggcggc
      961 cgcgagggcg tcgatgagcg aagccaggcc ggacagctcc tcggaggaga cgtcacgcat
     1021 ggacgccgct gtactcgaga ccgtgtcggc cgcgcctgcc aggcgcgtgc ctatctcgag
     1081 ggcaccggcc tgggcgtcgc tgacggcggc ggccatgcgc tcgaactgga tcctggccgc
     1141 gtcggcggag cggcgcgtgt cgtcgaccag ctccgactgc atcttgatcg ccatcgagct
     1201 gaagatgccc gcggcgatga cgatgacgcc gcaggtgcct aggttctgca tggccagctc
     1261 ggtgaccact ccgccgtcga ggggcaacgc gtctaggtag tagagcgccc cgatggcgag
     1321 caggttaagg agcgtcatga tccaagcctg gactggcttg gctgcgatta ggccggtggt
     1381 aacgagcat
//
LOCUS       DAOOLN010000651         1387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_124973_length_1387_cov_2.827327, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000651 DAOOLN010000000
VERSION     DAOOLN010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124973_length_1387_cov_2.827327"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..>1387
                     /gene="murJ"
                     /locus_tag="PLW80_08450"
     CDS             166..>1387
                     /gene="murJ"
                     /locus_tag="PLW80_08450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPB66579.1"
                     /translation="MSDESEATGRLVRESGKLSALTMVSRVLGLIRDRTRGALMGTGV
                     LADAFTVAFSVPNLFRRLFAEGSMSVAFIPTVKAYLSEGDREKTEEFLSSMFTALSLA
                     VCAVVAAGVAASPLLVGLFDSEPVETTILMRMMFPFLALVSIAAFFQGILNAYGSFTP
                     SGFAPILFNLSFIFVPMLLGGLAANPARAMAVGVIVGGTFQALCQLPYVLRFGARFGL
                     VSPRRAFSNPGVRRVLSLIAPTILGMAAYEVNGLVSTSLATRAGTGAATALSFSLRLQ
                     ELVLGVFVVSVGTVLLPELSGLAAEGDWKRYRERLTRALDAIALITVPVAVISILERI
                     DIVSLLFRTQAFGDDSVAQTASAFFFHMLGLPFIGANRILAPAFYARGDTRTPAWSGI
                     LAVGVNVAAAFALQP"
BASE COUNT          167 a          489 c          462 g          269 t
ORIGIN      
        1 tgatgacccg cggcatagcc gttaccgata cgaagtccgt ggccgcgagt ttcgccgagg
       61 ccctggccag ctactatatc ggtccgtagc gcccggtccc ggccgccgcg ggccggagga
      121 cgacgcgcct tgcggcgccg gcgcctttga cgctacagtg ccgtcatgag cgatgaatcg
      181 gaggcgaccg ggcgcctcgt ccgcgagagc ggcaagctgt cggcgctgac gatggtctcg
      241 cgggtgctcg gcctgatacg cgaccgtacg cgcggggcgt tgatgggcac gggcgtgctg
      301 gccgacgcct tcaccgtggc cttcagcgtg ccgaacctgt tccggcgcct gttcgccgag
      361 ggctcgatgt ccgtggcctt catcccgacc gtcaaggcct acctgtcgga gggcgaccgc
      421 gagaagaccg aggaattcct gtcgtcgatg ttcacggcgc tgagcctggc cgtctgcgcc
      481 gtcgtcgccg ccggcgtcgc cgcgtcgcct ctcctcgtcg gcctgttcga ctcggagccg
      541 gtcgagacga ccatcctgat gcgcatgatg ttcccgttcc tggcgctcgt gtcgatcgcg
      601 gccttcttcc agggcatcct gaacgcttat ggctcgttca cgccgtcggg cttcgccccc
      661 atcctgttca acctgagctt tatcttcgta ccgatgctgt tgggcggcct cgcggccaac
      721 ccggcgcgcg ccatggcggt cggcgtcatc gtaggcggca cgttccaggc tctgtgccaa
      781 ctaccgtacg tgctgcgctt cggcgcccgg ttcggtctcg tgtcgcctcg gcgcgccttc
      841 tcgaacccgg gggtgcgccg cgtgctgtcg ctgatcgccc cgaccatcct gggcatggcc
      901 gcttacgagg tgaacgggct cgtctcgacc tcactggcga cgagggccgg taccggcgcc
      961 gcgaccgcgc tcagcttctc gctacgcctc caggagctgg tgcttggcgt gttcgtcgta
     1021 tcggtcggca ccgtactcct gcctgagctg tcgggcctgg cggccgaggg cgattggaaa
     1081 cgctatcggg agcgcctgac gagggcgctc gacgccatcg ccctgatcac cgtgccggtg
     1141 gccgtcatct ccatcctcga gcggatcgac atcgtgtccc tgctgttcag gacccaggcc
     1201 ttcggcgacg attcggtggc gcagaccgcg agcgcattct tcttccatat gctcggcctg
     1261 ccgttcatcg gggcgaaccg catcctggcc ccggccttct acgcccgggg cgacacgagg
     1321 acgccggcct ggtccggcat cctcgctgtc ggtgtgaacg tcgccgcggc gttcgcgctg
     1381 caaccgg
//
LOCUS       DAOOLN010000652         1386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_125160_length_1386_cov_2.389932, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000652 DAOOLN010000000
VERSION     DAOOLN010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125160_length_1386_cov_2.389932"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..>1386
                     /locus_tag="PLW80_08455"
     CDS             17..>1386
                     /locus_tag="PLW80_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPB66580.1"
                     /translation="MYDYGAAKPSSGAAGSRGVTPSSSGLKAGYSDDNEQFNYFVGFL
                     EKYADVPHYGYDISERITVRVLDSAGKPVANAAVSIRSGGKELAAGLSYGDGSFRFYP
                     AAIGSKDGTFELRASAGGLSAGATTRRDGPRTVELRLQGPRSLPDPLPLDLLFVMDTT
                     GSMGEEIERLKATIEIIHDNLAALRPRPLVRLGLVLYKDLGDEYVTRLSPFTADLETF
                     RAALQPVVASGGGDGPEDLESALDDAVNRMDWNEGGLRLAFVVTDAEAHLDYGRDYTY
                     VDAANDARARAIKLYTIGTGGLPLEGEYLLRQVSQLTDARYIFLTYGEAGESDGGAEG
                     SVSHHTGSNYQTDKLEAIVIRFVKEEVAWLSDTPVSVDEGYFSARPVDEESRDETLGK
                     LFVEAVGNLADYSTFRFGPEAPCVILPVSATGEGLGPSAEYFGERLVLAASEAGAGVI
                     GGYTDCS"
BASE COUNT          232 a          462 c          472 g          220 t
ORIGIN      
        1 cccggcgccc gccgtcatgt acgactacgg cgcagccaag ccgtcgtcgg gagccgcggg
       61 atcgcgcggc gtcactcctt cttcgtccgg cctcaaggca ggctattccg acgataacga
      121 gcagttcaac tacttcgtcg gcttcctcga gaagtatgcc gacgtgccgc actacggcta
      181 cgacatctcg gagcgcataa ccgtgcgggt gctcgactcg gccggcaagc cggtggcgaa
      241 cgccgccgta tcgatacgct cgggcggtaa ggaactggcg gccggtttga gctacggcga
      301 cggatctttc cgcttctacc ccgcggcgat cggctcgaag gatgggacct tcgagctgag
      361 ggcctccgcg ggagggctga gcgccggcgc gacgacgagg cgggacggcc cgcgcaccgt
      421 cgagctccgg ctccagggac cgcgttcgtt gcccgaccca ctccccctcg acctactgtt
      481 cgtgatggat acgaccgggt cgatgggcga ggagatagag cggctcaagg cgaccatcga
      541 gattatccac gacaacctgg cggcgctacg cccgcgaccg ctcgtgcgtc tcggcctcgt
      601 gctatacaag gacctagggg atgagtacgt gacgaggctc tcgccgttca ccgccgatct
      661 cgaaacgttc cgcgccgccc tccagccggt cgtcgcttcc gggggcgggg acggccccga
      721 ggatctggag tctgccctgg acgacgcggt aaaccgcatg gactggaacg agggaggcct
      781 caggctggcc ttcgtcgtca ccgacgcgga agcgcatctg gactacggcc gcgactatac
      841 ctacgtcgac gcggccaacg acgcgcgggc cagggccatc aagctctaca cgattggcac
      901 cggcggcctg ccgctcgagg gggagtatct gctcaggcag gtatcgcagc tgaccgacgc
      961 caggtacatc ttcctcacct acggcgaggc gggagagtcg gatggcggcg ccgagggctc
     1021 cgtaagccac cataccggct cgaattacca gactgacaag ctcgaggcca tcgtcatccg
     1081 cttcgtcaag gaggaggtgg cctggctttc ggatactccc gtatcggtcg acgagggcta
     1141 cttcagcgcg aggccggtcg acgaggagag ccgcgacgag acgctcggaa agctgttcgt
     1201 cgaggcggtg ggcaacctgg ccgactactc gaccttccgc ttcggccccg aggcgccgtg
     1261 cgtgatactg cccgtgtcgg cgaccggcga aggcctcggg ccgagcgccg agtacttcgg
     1321 cgagcggctc gtcctggccg cgtccgaggc cggcgcgggg gtcatcggcg ggtacaccga
     1381 ctgctc
//
LOCUS       DAOOLN010000653         1385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_125289_length_1385_cov_2.728571, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000653 DAOOLN010000000
VERSION     DAOOLN010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125289_length_1385_cov_2.728571"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PLW80_08460"
     CDS             <1..532
                     /locus_tag="PLW80_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66581.1"
                     /translation="SASTAPLAARVAAVPGAREAVAALAALLPIAELAGGLAEAPKAR
                     ALAESAGLGRKLREAMAEAGLDPGLADAASGFAMAMLGRLGRVSERLGGGPGGISGRA
                     TARVILADDEARALFRVNRWNNESWIDADRCGSARRCTPWRLWRSAGSAPPRRSARCP
                     RPTGCSRARSGAWTAL"
     gene            688..1191
                     /locus_tag="PLW80_08465"
     CDS             688..1191
                     /locus_tag="PLW80_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HPB66582.1"
                     /translation="MAFYEISPRAVEGNVFDRVQKTWMLVTAGTKASWNTMTANWGGF
                     GHLWNRDVAFVFVRPTRYTYAFIERTGLLTLSFFGEEWRDALTVCGTMSGRDVDKAGL
                     TGLKPAEPRPGFIGFEQADLTFACRVIHKQDIDPAGFVDGSLEGHYDGDYHRVYVAEI
                     VAALARA"
     gene            1197..>1385
                     /locus_tag="PLW80_08470"
     CDS             1197..>1385
                     /locus_tag="PLW80_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66583.1"
                     /translation="MRSRLPEELLGELDDPSVRFVFPSEIAAASALTAALAATGRRAL
                     PSRRFLGWDAFKSEVFAGD"
BASE COUNT          211 a          470 c          494 g          210 t
ORIGIN      
        1 gtccgcctcc acggcgccgc tcgccgccag ggtcgccgcc gtaccgggcg cccgcgaggc
       61 cgtggccgcg ctcgcagccc tgctaccgat agcggaactg gcgggcggac tcgccgaggc
      121 cccgaaggcg cgggcgctag ccgagtcggc tggcttggga cgcaagctac gcgaggcgat
      181 ggccgaggcc gggctcgatc caggcctggc cgacgctgcc tccggcttcg ccatggcgat
      241 gctcggacgc ctgggcaggg tctccgaacg gctgggaggc ggcccgggcg gcatctcggg
      301 acgcgctacg gccagggtca tcctggccga cgacgaagcc cgcgccctat tcagggtgaa
      361 ccgctggaat aacgagagct ggatagacgc cgaccggtgc ggctcggctc gacgctgtac
      421 gccgtggcgg ctgtggcgtt cggcgggctc ggcgccgccg aggcggtccg ctcgctgtcc
      481 gcgaccgacc gggtgctcga gggctcgctc tggagcctgg accgcgctat aagcgcccat
      541 ggggcatcgg ccgcggctac gcgcccggct tctaacaagg cttctaacaa ggcttcgaat
      601 aaggcttcga ataaggcctc gagcaaggcg gcgggcgaag acgttcccac gaggctgccg
      661 acagacacga acagcgagga gtagcgtatg gcattctacg agatcagccc gcgggctgtc
      721 gaaggcaacg tgttcgaccg cgtccagaag acctggatgc tcgtcacggc cgggacgaag
      781 gcttcctgga acacgatgac cgccaactgg ggcgggttcg gccacctgtg gaaccgcgat
      841 gtcgccttcg tgttcgtgcg ccctacgcgc tacacctatg cgttcatcga aaggaccggc
      901 ctcctgaccc tgtcgttctt cggggaggaa tggcgcgacg cgctgaccgt ctgcggcacg
      961 atgtctggcc gcgacgtcga caaggccggt ttgaccgggc tcaagccggc cgagcctcgg
     1021 ccaggcttca taggtttcga acaggccgac ctgaccttcg cctgccgcgt gatccataag
     1081 caggacatcg acccggccgg cttcgtcgac ggctcgctcg aggggcacta cgacggcgat
     1141 taccaccggg tctacgtcgc ggagatcgtc gcggccctgg cccgcgcgta gagcccgtgc
     1201 ggagccgact gcccgaggag ttgctcggcg agctcgatga cccgagcgtc aggttcgtct
     1261 tcccgagcga gatagccgcc gcgtcggcgc tgaccgcggc cctggccgcc accgggagga
     1321 gggcgctgcc gtcccggcgc tttctcggct gggacgcgtt caagtccgag gtgttcgccg
     1381 gcgac
//
LOCUS       DAOOLN010000654         1385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_125293_length_1385_cov_2.603008, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000654 DAOOLN010000000
VERSION     DAOOLN010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125293_length_1385_cov_2.603008"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /gene="pheS"
                     /locus_tag="PLW80_08475"
     CDS             complement(<1..1000)
                     /gene="pheS"
                     /locus_tag="PLW80_08475"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF013568.2"
                     /note="catalyzes a two-step reaction, first charging a
                     phenylalanine molecule by linking its carboxyl group to
                     the alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; forms a heterotetramer of
                     alpha(2)beta(2); binds two magnesium ions per tetramer;
                     type 2 subfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPB66584.1"
                     /translation="MDPRTFLKGLHPLEIKVLLRYAPGDGLDAARLASELGYVEGHAN
                     QAQAWLSAKGLAAETGRSSRNVYELTALGREWLEAGSPEERMVAWLAQHGPAAMPTIC
                     QAVGIEQKDAGSAYGRLSKEGVLAMGQDKLVSLVDASKSLRAAALKALLAKAAAAGGI
                     LDEGALGDAERALMAEVAKKRGAADAAFKVAERETVTWALTADAAAVSALLREEGVSG
                     DELGAVTQELLESGSWKGAAFRSYNIKSRPARLIPGRRNPYVQFLESVKDKLVSLGFE
                     EFDGGLVETEFWNGDALFMPQFHSARDIHDVYYLADPTKAKSIEEPYQSRDVAGRVE"
     gene            complement(1060..>1385)
                     /locus_tag="PLW80_08480"
     CDS             complement(1060..>1385)
                     /locus_tag="PLW80_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66585.1"
                     /translation="GTRDHSRGVPVNFSQISNAEAIVEADLPDFRVDSSLGSHFFHNV
                     TAMNIGYFSAAYGSLSSFVDWDALAALPLVARTAHCSLVRAERELEILMDGGRGHGVI
                     RPSSD"
BASE COUNT          215 a          471 c          463 g          236 t
ORIGIN      
        1 gttccactcg gcccgcgaca tccctggact ggtagggttc ctctatggac ttggccttgg
       61 tcgggtcggc cagatagtac acgtcgtgga tgtcgcgggc cgagtggaac tgcggcatga
      121 acagagcgtc gccgttccag aattccgtct ccacgaggcc gccgtcgaac tcctcgaaac
      181 cgagcgagac gagcttgtcc ttgacgctct ccaggaactg cacgtagggg ttgcggcgcc
      241 ccgggatcag gcgcgccggc cggctcttga tgttgtacga cctgaacgcg gcgcccttcc
      301 acgagccgga ctcgagcagc tcctgcgtga cggcgcccag ctcatcgccg gacacgccct
      361 cttcgcgtag cagggccgag acggccgcgg catcggccgt gagggcccag gtgactgtct
      421 cgcgctcggc aaccttgaag gccgcgtcgg ccgccccgcg cttcttggcc acctcggcca
      481 ttagcgcgcg ctcggcgtcc ccgagggcgc cttcgtccag tatcccgccg gcggcggcgg
      541 ccttggccag cagggccttg agcgcggccg cccggagcga cttggacgcg tcgacgagcg
      601 agacgagctt gtcctgcccc atcgccagga cgccttcctt ggaaaggcgg ccgtaggccg
      661 agccggcgtc cttctgctcg atgccgacgg cctggcagat ggtaggcatc gcggccggac
      721 cgtgctgagc cagccacgcg accatgcgct cctcgggcga gcccgcctcg agccactcgc
      781 ggccaagcgc ggtcagctcg tagacgttgc gcgacgagcg gcccgtctcg gcggccaggc
      841 ccttggcgct cagccaggcc tgggcctggt tggcgtgccc ctcgacgtag ccgagttccg
      901 acgcgagcct ggccgcgtcg aggccgtctc ccggcgcgta tctgagcagt acctttatct
      961 cgagcgggtg caagcccttg aggaacgttc gtggatccat gatgccttct ccagcgtctg
     1021 ctattgttgc tacgatagtg tcggaattcg ccgtcgtcgt caatcggagg acgggcgtat
     1081 gacgccatgc ccccgccccc cgtccattag gatctccagt tcccgttcgg cgcggacgag
     1141 cgagcaatgc gccgtacgcg cgacgagcgg cagggctgcc agagcgtccc agtcgacgaa
     1201 ggacgatagc gagccgtagg cagcggagaa gtacccgatg ttcatcgcgg taacgttatg
     1261 gaagaaatgg ctgccgagcg acgagtctac cctgaagtcc gggaggtcgg cctcgacgat
     1321 ggcctcggcg ttcgatatct gagagaaatt aaccggcacc ccgcgcgaat ggtcgcgcgt
     1381 acccc
//
LOCUS       DAOOLN010000655         1384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_125411_length_1384_cov_3.107600, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000655 DAOOLN010000000
VERSION     DAOOLN010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125411_length_1384_cov_3.107600"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PLW80_08485"
     CDS             complement(<1..603)
                     /locus_tag="PLW80_08485"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPB66586.1"
                     /translation="MGKRAYCILVALFACALSAPIVAQDAPAAKPATEYQGADGLENW
                     TTEYDVSGLKPGTYNLLARAIDAAGNVTFAVPFDLIVDPASDLPIAGIVNPLHLSRVG
                     ADLNVVGTCVDDDAVSRVELRLDDGEWVRAVGSDYWSYYLPTAGVADGLHTISARGVD
                     SNGLVGGESTVSFHLDRTKPLHEVAKPAFGAIASGRLAVTG"
     gene            828..>1384
                     /locus_tag="PLW80_08490"
     CDS             828..>1384
                     /locus_tag="PLW80_08490"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPB66587.1"
                     /translation="MSDDINFTVPDLGARTVRSPLAFSLSSGDAIANYVDDADRVRWN
                     SSARVSGDAEAAANLLEKAGPRERLYFNPAHVHAGVVTCGGLCPGLNDVIRALVRCLW
                     NRYGVRRVSGVRFGYKGFLPEYGLPVKELTPDVVDDIHKIGGTILGSSRGGGERTAEI
                     VDAIERMNLNVLFAIGGDGTQKGAMA"
BASE COUNT          233 a          449 c          463 g          239 t
ORIGIN      
        1 gccggtcacc gccagtcgcc ccgacgctat ggccccgaac gccggcttgg ccacctcgtg
       61 gaggggcttg gtccggtcca ggtggaagga gaccgtgctc tccccgccga ccaggccgtt
      121 ggagtcgacg ccgcgggccg agatggtatg gagcccgtcg gccacgccgg cggtgggcag
      181 gtagtacgac cagtagtcgg agccgaccgc ccgcacccac tcgccgtcgt cgagcctgag
      241 ctccacgcgg ctcaccgcgt cgtcgtcgac gcaggtaccg acgacgttca ggtcggcgcc
      301 gacccgggag agatggagcg ggttgacgat gccggctatc ggcaggtcgg aggcaggatc
      361 gacgataagg tcgaacggga cggcgaaggt cacgttcccg gccgcgtcga tcgccctggc
      421 caggaggttg tacgtgcctg gcttgagtcc cgatacgtcg tattcggtcg tccagttctc
      481 gagcccgtcg gcgccctggt actccgtagc cggtttggcg gccggagcgt cctgcgcgac
      541 gatcggtgcc gatagagcgc aggcgaacag agcgacgagt atgcagtatg ccctcttacc
      601 catgacggac tccctggaac gagcggatgt gtctgcctct agtatcggca gcagctacca
      661 tgggttacat actacatcga tgcgacgaaa ttgtagcaca aatatgtata aaccgtgcta
      721 gttctttcga ttgagaaagg agtgcttatc cgctatactc tctgccagcg tgaaaccgga
      781 tccgcccgtc tctcggcctc cttggcgccc ggttctaagg atgggacatg tccgacgata
      841 taaacttcac cgttccggat ctcggcgcgc ggaccgtgcg ctcgccgctg gccttttcgc
      901 tgtcctcggg cgacgccatc gccaactacg tcgacgacgc cgaccgggtg cgctggaatt
      961 cgagcgcccg cgtctccggc gacgccgagg cggcggcgaa cctcctcgag aaggccgggc
     1021 ccagggagcg gctgtacttc aacccggcgc acgtacacgc cggcgtcgtg acctgcggcg
     1081 gcctttgccc ggggctcaac gacgtgatcc gcgccctggt acgctgcctg tggaaccgct
     1141 acggcgtcag gcgcgtctcc ggcgtgcgct tcggctacaa gggtttcctg cccgagtacg
     1201 gcctgccggt caaggagctg acgcccgacg tcgtcgacga tatccataag ataggcggca
     1261 ccatactcgg ctcgtcgcgc ggcggcgggg agcgcaccgc cgagatagtc gacgcgatag
     1321 agcgcatgaa cctgaacgtg ctgttcgcca tcggcggcga tggcacgcag aagggcgcga
     1381 tggc
//
LOCUS       DAOOLN010000656         1384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_125416_length_1384_cov_2.950339, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000656 DAOOLN010000000
VERSION     DAOOLN010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125416_length_1384_cov_2.950339"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..>1384
                     /locus_tag="PLW80_08495"
     CDS             18..>1384
                     /locus_tag="PLW80_08495"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPB66588.1"
                     /translation="MQKLFKSTLRHPFIVMSLALGITALAVLSVVSGFRMETNLDTYM
                     PRTHPAFVFSDEADERFMIKDSILIAVEHPYSVFNPATLQKLIDIEDDLLALEGLSGA
                     RVNSIHTGDNILATEDGLDVRRFYTDAPIDQEGADRIASAVRGNSMIAGRLVSHDERT
                     ALIIVELPESGFSRDLYDQIQQIADERTGPETLHIAGRPIVEGTLAALGPADMARMGP
                     LVLLVIAIVLLLVLRQVRRTIITMSVVVFSTIWAFGLMSALGVPMYSVSIMIPVMLIA
                     IGVAYAIHLYNQIDHFARETPGAGRRDIAENAITVISSPALFAALTTVAGFVSLVTSR
                     VYPVKYFGLFTAFGVFASFVLTMLLVPAGIMAFGNGDVKKAAGSHAGRIPEHGLGARF
                     ADALKARPGRVYVIVAVLVAASVFGISRVWINTSFLANFDSNSAIVKTDAFVNEHFGG
                     TSSLNV"
BASE COUNT          265 a          456 c          391 g          272 t
ORIGIN      
        1 caccaaaggg gttgccaatg cagaagctat tcaagtccac cctgcgccat ccgttcatcg
       61 tgatgtcgct agctctgggc atcaccgcgc tcgcggtgct ttccgtggtt tcagggttca
      121 ggatggaaac caacctggat acctacatgc cccgcacgca tccggcgttc gtgttcagcg
      181 acgaagccga cgagcgcttc atgataaagg actcgatcct catcgccgtg gagcatccct
      241 attcggtgtt caatccggcg acgctccaga aactgatcga catcgaggac gacctcctgg
      301 cgctggaagg actgtccgga gcccgcgtca actcaatcca tacgggcgac aacatcctgg
      361 ctacggaaga cggactggac gtgcgcagat tctataccga cgctcctata gaccaggaag
      421 gcgccgaccg catagccagc gccgtccggg gcaacagcat gatagccggc cgtctcgtct
      481 cccatgacga gcgcacggcc ctcataatcg ttgaactgcc tgaatccggt ttctcgcggg
      541 atctgtatga tcagatccaa cagattgcgg acgagcgaac gggaccggaa accctccaca
      601 tagccggccg gccgatagtc gaaggcacgc tcgccgcgct cggccccgcg gacatggcca
      661 ggatgggacc cctggtactg ctcgtcatag ctatcgtcct cctcctggtc ctcaggcaag
      721 tacggcgtac catcatcacg atgtcggtcg tcgtgttcag tacgatctgg gctttcgggc
      781 ttatgagcgc gctcggcgtc cccatgtact cggtcagcat catgataccg gtgatgctca
      841 tcgccatcgg cgtcgcctat gccatccacc tgtataacca gatcgaccac ttcgccaggg
      901 aaacgcctgg agcgggccgg cgcgacatcg cggaaaacgc cataacggtc ataagtagcc
      961 cggccctgtt cgcggcgctg acgacggtcg ccgggttcgt gtcgctcgtc acctcgcggg
     1021 tctacccggt caagtacttc gggctcttca ccgcgttcgg cgtcttcgcg tcattcgtgt
     1081 tgacgatgct cctcgtgccg gcgggcatca tggccttcgg caacggcgac gtcaaaaaag
     1141 ctgccggttc ccacgccggg cgcatacccg aacacggact cggcgcacgg ttcgccgatg
     1201 ccctcaaggc caggcccggg cgcgtctacg tgatcgtcgc ggtcctggtc gcggcttccg
     1261 tattcggaat ctcccgggta tggatcaata ccagtttcct ggccaacttc gactcgaaca
     1321 gcgccatcgt caagaccgac gccttcgtca acgagcattt cggcggcacg tccagcctta
     1381 acgt
//
LOCUS       DAOOLN010000657         1384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_125426_length_1384_cov_2.754703, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000657 DAOOLN010000000
VERSION     DAOOLN010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125426_length_1384_cov_2.754703"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /gene="miaB"
                     /locus_tag="PLW80_08500"
     CDS             complement(<1..1186)
                     /gene="miaB"
                     /locus_tag="PLW80_08500"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPB66589.1"
                     /translation="MRFFIETYGCQMNKAESASFHLQFMERSWEPAATAEEADLVLIN
                     TCSVRITAENRAWGRIAHYAAMKRERAFTLVVTGCMAERLRERMREREPRIDYALGNF
                     QKAAFGLLLDAAAAGRRLETIEESPAFAFMPSHREPGSFSSFVPIMHGCDNFCSYCIV
                     PYVRGREVSRDPSSIAAEIESAAAAGVREITLLGQNVNSYRWRGLGGELDFAGLLTSI
                     APIARSAGIGRVRFVSSHPKDLSDATIEAIASDDVFARHIHLCVQHGSDRVLEAMNRR
                     YTRDSYTLLADRIRSRISGVTLSTDILIGFPGETEEDVEDTLELMRKLRFAYAFMYHY
                     NPREGTPAAVMPGQVPVAVKKARLARVIEAQKTITRSVMDGMIGSEAVVLAEAVSKRR
                     GDE"
BASE COUNT          257 a          438 c          441 g          248 t
ORIGIN      
        1 cctcgtcgcc tcttcgtttc gagacagcct cggcgagcac gacagcttcc gatcctatca
       61 tgccgtccat gacgctcctc gttatcgtct tctgcgcctc tatgacgcgg gccagcctgg
      121 ccttcttgac ggccacgggt acctgcccag gcataaccgc ggcgggcgtg ccctcacgcg
      181 gattgtaatg gtacatgaag gcgtaggcga agcgtagctt gcgcatcagc tccagggtat
      241 cttcgacgtc ctcttcggtc tcgccgggga atcctatgag aatatcggtc gaaagggtga
      301 cgcccgatat acgcgagcgg atacgatcgg ccaagagcgt ataggagtcg cgcgtgtacc
      361 tacggttcat cgcctcgagc acgcgatcgg agccgtgctg gacgcacagg tgtatgtgcc
      421 tggcgaagac gtcgtctgac gcaatcgcct ctatcgtcgc gtcggacaag tccttgggat
      481 ggctcgagac gaagcggacg cggcctatgc cggctgacct ggctatgggg gcgatagacg
      541 tcaggaggcc cgcgaagtcg agctcgccgc ccagaccgcg ccatcggtac gagttgacgt
      601 tctggcccag caacgtgatc tcgcgaacgc ccgctgcggc ggccgattct atctcggcgg
      661 ctatcgacga cgggtcgcgc gatacttccc gccctcggac atagggcacg atgcagtagg
      721 agcagaagtt atcgcagccg tgcatgatcg ggacgaagga gctgaacgag cccggctcgc
      781 gatgcgatgg catgaaggcg aaggccggcg actcctcgat cgtctcgagt cgccgtccgg
      841 cggcggccgc gtcgagcaga agtccgaagg ccgccttctg gaagttgccg agcgcgtagt
      901 ctatcctagg ctcgcgctct cgcatgcgct ccctgagccg ctctgccata caaccggtta
      961 cgaccagcgt gaaggcgcgc tcgcgtttca tcgccgcgta atgggctatg cgaccccaag
     1021 cgcgattctc ggcggtgatg cgcaccgagc aggtattgat caggacgagg tcggcctctt
     1081 ccgccgtagc cgccggctcc cagcttctct ccatgaactg aagatggaac gacgccgact
     1141 cggccttgtt catctggcaa ccgtaggtct ctatgaaaaa gcgcatgatc tccccggata
     1201 cggatcgggc ggccgacaga gcggccgccc gcgatgaggc cagagtcgcg acggagccta
     1261 gcccctggac ttgagcccct tgacgaactt gaacaggttg taggccccga agccgaccag
     1321 gccgacgccg gcggcgccga ggatgaagcc ggtaaagccc ttgagcacga acgacgcgat
     1381 gccg
//
LOCUS       DAOOLN010000658         1383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_125614_length_1383_cov_2.248494, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000658 DAOOLN010000000
VERSION     DAOOLN010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125614_length_1383_cov_2.248494"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="PLW80_08505"
     CDS             complement(<1..1247)
                     /locus_tag="PLW80_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66590.1"
                     /translation="MLEREYGAVSREGLVSLLSPGADGAASWLGSYYASEGQDAGTTM
                     ESLIVAAAGEAGDAWSYEAARSRVTAMVVDSLDTLASHIREARGHQGDASAPIERYLA
                     AIVASTAPDFEFVSWILPLVGLAGSYGQSSSLGASEDAAMDSAFIGLGDTAMAFSAAS
                     FYLAGLAEAGRTSEFDSLASLVSAEKAERGRAVIDRAEACSAYLGDFSGGIIAWALKA
                     SGGDVGMALAMVASIEGASSSNSLEGPFGRWLFALTSLKIDASFAEAERLKDLSAYSA
                     AYDRHIAEAEAAEAGGILHWRELLGSNALRAAIEADASRLPEPRAPGEPEAEPAETPA
                     VAASWNAGALADGSDAAHRRTLALNDGLASWKRYSESIGMVGGDQLASLVVSMERYTS
                     DPSTPYDASGLPRASSSVARASQA"
BASE COUNT          259 a          475 c          430 g          219 t
ORIGIN      
        1 gcctgactag ctcgggccac cgagctcgag gcgcggggca aacccgaggc gtcgtagggc
       61 gtggatggat cgctggtata ccgctccatg gacactacaa ggctagcaag ctggtcacca
      121 cccaccatgc cgatactctc cgagtatcgc ttccacgagg cgaggccatc gttcaaggcc
      181 agggtccgcc tatgtgccgc gtccgacccg tcggcgagcg cgccggcgtt ccatgaggcc
      241 gcgacagccg gcgtttcggc aggctcggcc tcgggctcgc ccggagctcg aggctcaggc
      301 aggcgcgacg catcggcttc aatggccgcc cgtaaggcgt tagacccaag caactctcgc
      361 caatggagaa ttccgccagc ctcggccgcc tcggcttccg ctatatgacg atcgtaggcg
      421 gccgaatagg ccgacaagtc cttcaggcgc tcggcctcgg cgaagctagc gtctatcttc
      481 aggctcgtca gcgcgaatag ccaacgaccg aacggcccct ccaacgagtt ggaagaagac
      541 gccccttcta tggacgctac catcgcgagg gccatgccga cgtcacctcc gctggctttc
      601 aaggcccagg cgatgatgcc cccggagaaa tcgcccaagt aggcggagca agcctcggcg
      661 cggtcgatca cggcccgccc gcgctcggcc ttctccgcgc ttacgagcga cgccagcgaa
      721 tcgaactcgg aggttcgccc cgcctcggct agaccggcta ggtaaaacga ggccgcgctg
      781 aaggccatcg ccgtatcgcc gagcccgatg aaggccgagt ccatcgccgc gtcttccgaa
      841 gctccgagag acgaagattg gccatacgaa ccagcaagtc cgacgagggg caagatccac
      901 gagacgaact cgaagtctgg agcggtcgag gcgacgatag cggccaggta gcgttctatc
      961 ggagccgacg cgtctccctg gtgtccacga gcctcgcgta tatgggatgc gagtgtatcg
     1021 agcgaatcga cgaccatggc tgtcacccga gatcgcgcgg cctcgtagct ccaagcgtct
     1081 ccggcctcgc cggccgccgc gacgatgagc gattccatcg tcgtcccggc gtcttggcct
     1141 tcgctggcgt agtacgatcc tagccagctt gccgctccgt ccgctccagg cgacaggagc
     1201 gacacgaggc cctcacgcga tacggcccca tattctcgtt cgagcatggc gaaaaccatc
     1261 gactccatcg tataggcgat tcgagccagc gggccagtgg cgaggcgggc ggcttccgcc
     1321 gtcgccgcgt agtcccacga cgaccaggcg tcctcgagcc gtatacgcgc agcatccttg
     1381 acg
//
LOCUS       DAOOLN010000659         1382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_125764_length_1382_cov_2.071590, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000659 DAOOLN010000000
VERSION     DAOOLN010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125764_length_1382_cov_2.071590"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1382)
                     /locus_tag="PLW80_08510"
     CDS             complement(<1..>1382)
                     /locus_tag="PLW80_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB66591.1"
                     /translation="LLLSLTGGGVRHALALILGAAAAAMECVLAARRISTSPLDDGLG
                     ARRVAAAIATRALAALAFAAVIAFGSQRLASLALPTWLALAVSLAAGERLSAIATRSR
                     ASHAESKRLLARLESETRGGVDFMTATAAALRGPLHGMMGMLEDLEALVQDAPLPSRA
                     ASDLAIARAEAARLDILVSNILSYSGLGPNRLADENLDLASVARSAASLLRVSIAGKG
                     VRVAVDAHVVEIRGDIGVAHRLLYTAMSHASRITGASLVRVSASSDESRVRVYVEDDG
                     GALDSPSGTVVDKGQERIELDIDAVVLGRLAGLLGGTYRFERQSGMNVRVFDLPRLAP
                     PGPADSRPRAMSEFPSSDDDGSGGPARPAGRVLVAGNEPVALLSIKRRLEASGWSVHA
                     TVSSADALDRALGRPPARAAAAQPYDIVIIDSAMPGMSGFEFCESVRRSKSPEALPIV
                     VLLEPGRVDE"
BASE COUNT          237 a          480 c          477 g          188 t
ORIGIN      
        1 ctcgtcgacc cgacccggct cgagtaggac gacgatgggg agcgcctcgg gcgacttgct
       61 gcggcgcacc gactcgcaga attcgaagcc gctcatgccc ggcatggccg aatctatgat
      121 gacgatgtcg tagggctggg cggcggcggc ccgagccgga ggccttccga gcgccctatc
      181 gagcgcgtcg gcggatgaca cggtagcgtg caccgaccag cccgaggcct cgagccgacg
      241 cttgatcgac aggagagcga ccggttcgtt ccccgcgacg agcacccgcc ccgccggacg
      301 ggccggcccg cccgacccgt cgtcgtccga ggatgggaac tccgacatgg cgcgcggcct
      361 cgagtccgcg gggccgggcg gagcgaggcg gggcaggtcg aacacgcgta cgttcatgcc
      421 ggattgccgc tcgaagcggt aggtgccgcc gagcagtccc gcgagccggc ccaacacgac
      481 agcgtcgata tcgagctcga tgcgctcttg gcccttatcg acgacggtcc ccgacggcga
      541 gtcgagcgct ccgccgtcgt cctccacgta gacccgtacc cgactctcgt cggaagaagc
      601 cgagaccctg accagggagg cgccggttat cctcgaagcg tgactcatcg ccgtatagag
      661 caggcggtgg gccacgccta tgtctccgcg tatctccacg acgtgcgcgt cgacggcgac
      721 tcggacgcct ttgccggcga tggaaacgcg aaggagactt gccgcggacc gagccacgga
      781 agccaagtcg aggttctcgt ccgccagcct gttcgggccc agacccgagt agctgaggat
      841 attcgatacg agtatatcga ggcgagccgc ctcggctcga gcgatggcta ggtccgatgc
      901 ggcccgggac ggtaacggag cgtcctggac cagggcttca agatcctcga gcatgcccat
      961 cataccgtgg aggggtccac gtagcgcggc cgccgtcgcc gtcatgaagt cgacgccgcc
     1021 cctcgtctcc gattccagcc tcgccaggag ccgcttcgac tcggcgtgcg aggcgcggga
     1081 ccgcgtggcg atggccgaca gcctctcgcc ggccgccagg ctgacggcaa gcgccaacca
     1141 agtcggaagg gccagcgagg ccagtcgttg cgatccgaag gctatgacgg ccgcgaaggc
     1201 tagggcggca agcgccctgg tcgctatggc cgccgcgact cgacgagcgc cgagcccgtc
     1261 gtccagaggc gaagtggaaa tccttctcgc cgccaggacg cactccatcg ccgcggccgc
     1321 cgcgccgaga atcagggcca gagcatgccg aaccccgccg ccggtcaggc tgagcagcag
     1381 cg
//
LOCUS       DAOOLN010000660         1381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_125847_length_1381_cov_3.137255, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000660 DAOOLN010000000
VERSION     DAOOLN010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125847_length_1381_cov_3.137255"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..440
                     /locus_tag="PLW80_08515"
     CDS             120..440
                     /locus_tag="PLW80_08515"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPB66592.1"
                     /translation="MPVTLINDTTIQTLLVVGPATVAEIDELGDAFRSAVASGAPAVR
                     VDLSGLGKLDVTFLQLLVSLRATLARAGRSLSCLGLGPDHPARRQAAALGLSAAFDGC
                     GGQR"
     gene            437..1285
                     /locus_tag="PLW80_08520"
     CDS             437..1285
                     /locus_tag="PLW80_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HPB66593.1"
                     /translation="MNFEDNKGLFVEEARELLESIESRLLELEQAPGDEAIIDDVFRS
                     LHTIKGSGAMFGFEEMTAFAHGLESLFDDVRKGRLGVTGEIVGLTLESVDCLSSLLEG
                     RDDEAARARGSIESRLEAVRRGAEPAPEADAKPPAAAGSSATDKAGTAGGSKGIVPIA
                     ADDPSEAGLTNVYRIRFRPDADLLGRGVKLENLFSELAELGAVVAVADTTRVPELDAL
                     EPSSLYLSWILTLSTTASEADVSSVFMFVEDYAELKIERLPVVDAEGKPAVLPIGVSC
                     TFWAAK"
BASE COUNT          217 a          467 c          460 g          237 t
ORIGIN      
        1 cgccccgcgc ctgggctgag aacgcctcga tgtgaatgga ttaaattaat atatcgtgtt
       61 aaataaataa attttacatt atagttacaa gttacgtata ggtgatatac gataccggta
      121 tgccagtaac tttaattaat gatacgacta tccaaactct gctggtggtc ggtccggcga
      181 ccgtcgccga gatcgacgag ctcggcgacg ccttccgctc ggccgtcgcc tcgggcgccc
      241 cggcggttcg ggtcgacttg tcaggcttag gcaagctcga cgtcaccttc ctgcagctcc
      301 tcgtatcgct ccgcgccacg ctggcccgtg ccggccggtc gctgtcctgc cttggcctgg
      361 gacccgacca tccggcgcgc cgccaggccg ccgccctcgg cctttcggcc gccttcgacg
      421 gctgcggagg ccagcgatga acttcgagga caacaagggc ctcttcgtgg aggaagcgcg
      481 cgagctgctc gagtccatcg agtcgaggct gctcgagctc gagcaggcgc ccggcgatga
      541 ggccatcatc gacgacgtgt tccgctcgct ccataccatc aagggctcgg gcgccatgtt
      601 cggcttcgag gagatgaccg ccttcgccca tgggctcgag agtctgttcg acgacgtgcg
      661 caaggggcgg ctcggcgtca cgggcgagat cgtcggcctg accctcgagt cggtcgactg
      721 cctgtcgtcg ctgctcgagg gccgcgacga cgaggcggcc agggcgcggg gctcgataga
      781 gtcgcgtctg gaagccgtca ggcgcggcgc cgagcctgcg cccgaggcgg acgccaagcc
      841 cccggccgcg gccggctcga gcgcgacgga caaggccgga accgcgggcg gttccaaggg
      901 catcgtgccg atagccgccg acgacccgtc cgaggccggc ctcacgaacg tgtaccgcat
      961 ccgcttccgg ccggacgccg acctcctcgg gcgcggcgtc aagctggaga acctgttctc
     1021 cgagctggcc gagctgggcg ccgtcgtcgc cgtcgccgat acgacgcggg tgcccgaact
     1081 cgacgcgctc gagccgtcgt cgctctacct gtcctggatc ctcaccctgt ccacgacggc
     1141 gagcgaggcg gacgtctcgt cggtcttcat gttcgtcgag gactacgccg agctcaagat
     1201 cgagcggctg cccgtggtcg acgcggaggg caagcccgcc gtgctgccga tcggcgtgag
     1261 ctgcacgttc tgggcggcga agtaggcgtt ggcgagctcg accagcttcc cgccggacga
     1321 cagcgtcgtg acctggccgc cgtgctcgcg cgccaccacg tcgatccgga tcgagggcag
     1381 c
//
LOCUS       DAOOLN010000661         1379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_126143_length_1379_cov_2.791541, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000661 DAOOLN010000000
VERSION     DAOOLN010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126143_length_1379_cov_2.791541"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PLW80_08525"
     CDS             complement(<1..400)
                     /locus_tag="PLW80_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HPB66594.1"
                     /translation="MIINHNLSAMFANRSLGVTNSGVEKNMEKLSSGMRINRAGDDAS
                     GLAVSEKMRSQIRGLNMASKNAANGISFIQSTEGYLQETQDIIQRLRELAVQASNGIY
                     TSEDRMQIQVEVSQLVDEIDRIASHAQFNGM"
     gene            732..1091
                     /locus_tag="PLW80_08530"
     CDS             732..1091
                     /locus_tag="PLW80_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66595.1"
                     /translation="MKVRDIVSTLEGVLLTGEALLDLDIQAACGADLMSDVMAFVKEN
                     VVLLTGLVNPQSIRTADLMDIKVVVFVRGKVPPKDMVAEAERNGMAVIATKYSMFLAC
                     GRLYEAGIHGGGTRDIR"
     gene            1096..>1379
                     /locus_tag="PLW80_08535"
     CDS             1096..>1379
                     /locus_tag="PLW80_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HPB66596.1"
                     /translation="MKLRYEVSGEDMSLAGKASGEVKRALGRLGVNPAAIKRTAVCMY
                     EAEMNMVIHGGGGVAEAELSPDRVVVTMRDSGPGIPDLELAMQEGYSTAP"
BASE COUNT          232 a          417 c          452 g          278 t
ORIGIN      
        1 tcatgccgtt gaactgggcg tggctggcga tgcggtcgat ctcgtcgacg agctgagaga
       61 cctctacctg gatctgcatg cggtcttccg aggtatagat accgttggag gcctgcacgg
      121 cgagctcgcg gaggcgctgg attatgtcct gcgtctcctg gaggtagccc tcggtgctct
      181 ggatgaacga gatgccgttg gcggcgttct tggaggccat gttgaggccc ctgatctgcg
      241 aacgcatctt ctcagacacg gcgagtcccg aagcgtcgtc gccggcgcgg tttatccgca
      301 tccccgacga tagcttctcc atgttcttct ctactccgct gttcgtgacc cctagagagc
      361 gattggcgaa catcgcacta aggttgtgat tgatgatcat aacccgatct cctttccata
      421 gaagcgacgg cctacggccg tctcggcatc cgtgccgaaa tttacgtgtc gatttctccg
      481 gtacgctgct ccgggccgca cccgtgaacg tgaaccgaat agagcccgcc cgtggacgga
      541 gctaggcgat cgcgcgttcg cttcgcttcc gtcccgtagc ggtcccacac ttaaagctat
      601 cggcgtttgg ctcgcggcct ttagccccgt tcgtagccga ctgacgagag gtcgcgggct
      661 ctcggcttgt ccggcttcgc ggcgcgtgtt agactcgtcc agtcgccgga cgaacgggcg
      721 ctggaggatt catgaaggtt cgggatatcg tttctaccct cgagggagtc ctcttgaccg
      781 gcgaggccct gctcgacctg gacatccagg ccgcctgcgg cgccgacctg atgagcgacg
      841 tgatggcgtt cgtcaaggag aacgtggtac tgctgaccgg gttggtcaat cctcagtcca
      901 tccgcacggc cgacctgatg gacatcaagg tggtcgtgtt cgtccgcggc aaggtgccac
      961 ccaaggatat ggtggcagag gccgagcgca acggcatggc ggtcatcgcg accaagtact
     1021 cgatgttcct ggcctgcggc cggctgtacg aggcgggcat ccacggcggc gggacgcgag
     1081 atatccgcta ggctcatgaa actccgttac gaggtgtccg gtgaggacat gtccctggcg
     1141 ggcaaggcga gcggcgaggt caagcgcgcg ctcggccggc tcggagtgaa ccccgccgcc
     1201 atcaagcgta ccgccgtctg catgtacgag gcagagatga acatggtcat ccacggcggc
     1261 ggcggggtgg ccgaggccga gctcagcccg gaccgcgtcg tcgtcaccat gcgggattcc
     1321 gggccaggta ttcctgacct cgagctggcg atgcaggagg gctattcgac ggctccaga
//
LOCUS       DAOOLN010000662         1379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_126145_length_1379_cov_2.771903, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000662 DAOOLN010000000
VERSION     DAOOLN010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126145_length_1379_cov_2.771903"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PLW80_08540"
     CDS             complement(<1..297)
                     /locus_tag="PLW80_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66597.1"
                     /translation="MERTKNLKADPKALAAAEAKQEGKRLAGEYLADPTACDFQSRFA
                     AIAKDRREAAREGAFDVFVSRIQLPTSAVAQPEAELAPVAEGLKALNVAPFGEKK"
     gene            complement(239..1186)
                     /locus_tag="PLW80_08545"
     CDS             complement(239..1186)
                     /locus_tag="PLW80_08545"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPB66598.1"
                     /translation="MPIEQPVTAKPWKRLDTLTRSFIAVLASYQLAQFAVFSAYALPL
                     GFFGAFALPFALSSMGFHLLTFALLVVFRNDFVIEPAGRRLERVNLANGITLFRVSTL
                     PTVLFVILASRDYPVRFSLVALVAIVFATDFLDGFVSRRYNETTRVGRMMDSASDYAL
                     LFVISLVFYYFRIIPGWFLYLLVGRLVGQTLMVLAVLLVKKRVTPRTSFLGKATVAST
                     MALYAFELLRFVADLPPAAYSALELVVGAVVAVSILDKLRIMLQDLRAAPVTAAGPGR
                     LIPTNDGDTHGDDQERARDSDGTNQEPEGGSQGPGGRRG"
BASE COUNT          263 a          413 c          458 g          245 t
ORIGIN      
        1 cttcttctcg ccgaaggggg cgacgttcag ggccttgagg ccctcggcga cgggggcgag
       61 ttcggcctcc ggctgggcca cggcgctggt cggtagctgg atcctcgaga cgaatacgtc
      121 gaaggcgccc tcccgcgcgg cttcgcgcct gtccttggct atggccgcga accgggactg
      181 gaagtcgcag gccgtcgggt ccgccaggta ctcgccggcc aggcgcttac cttcctgctt
      241 agcctcggcg gccgccaggg ccttgggatc cgccttcagg ttcttggttc gttccatcgc
      301 tatctcgagc gcgctcttga tcatcgccat gcgtgtctcc atcgtttgtc ggtataagcc
      361 tgcccggtcc ggcggccgtc accggggcgg cgcgcaggtc ctgcagcatg atgcgcagtt
      421 tatccagaat cgagaccgcg acgacggccc cgacgacgag ttccagcgcc gagtacgcgg
      481 ccggcggcag gtcggccacg aagcgcaata gctcgaacgc gtacagggcc atggtcgaag
      541 cgaccgtggc cttgcccaag aacgaggtcc gcggcgtgac gcgcttcttg acgagtagca
      601 ccgccaacac catcagggtc tgaccgacca gcctcccgac caacaggtac aggaaccatc
      661 ccggtatgat ccgaaagtaa taaaagacga ggctgatgac aaacaggagc gcatagtcgc
      721 tcgccgagtc catcatccgg ccgacccggg tcgtttcatt atagcgccgc gacacgaagc
      781 catcgaggaa gtcggtggcg aagacgatgg cgaccagggc gacgagggag aagcggactg
      841 ggtagtcgcg gctggccagg atcacgaata gcaccgtcgg cagggtgctg accctgaaca
      901 gggtgatgcc gttggccagg ttgacgcgct cgagcctgcg tccagccggc tctatcacga
      961 agtcattcct gaagacgacg agcagggcga aggtgagcag gtggaagccc atcgagctta
     1021 acgcgaatgg tagcgcgaag gcgccgaaga agccgagcgg tagggcgtag gccgagaaga
     1081 cagcgaactg cgccaactga tacgaggcga ggacggcgat gaagctacgg gtgagcgtat
     1141 cgagccgttt ccatggctta gcggtaacag gctgttcgat gggcatatgc gtccttatcg
     1201 gccgatacga gccggggaaa tgatacaact tgacaccagc ggtgtcaaat ggacatactt
     1261 ttagtatgaa cgtcgcagat gcccttaccg cgagccggct ttttatggct cctttgttct
     1321 tcgtcgtgtt catctggggc gcttcgctcg ggttgccggc cggcgcctcg tccggcgcg
//
LOCUS       DAOOLN010000663         1379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_126157_length_1379_cov_2.540785, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000663 DAOOLN010000000
VERSION     DAOOLN010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126157_length_1379_cov_2.540785"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PLW80_08550"
     CDS             complement(<1..799)
                     /locus_tag="PLW80_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HPB66599.1"
                     /translation="MKTIVLDGRGLAIGDVALAARFGAKAELAREAIPRMQASRAVVE
                     RCVDESLIRYGITTGFGKFCDVVISRESNAELQRNLIMSHAVGVGDPLPEEVVRAMML
                     LRVNALAVGNSGARLSTAQALLDMLNAGVTPVVPEKGSLGASGDLAPLSHVAIALCGM
                     GECCYKGRRMSSAEALAGAGLAPVELAAKEGLALINGTQCMTALAALAAYDAERLALT
                     ADVVAALTSQALRGIVDAYDPRVHEVRGQPGQIACATRLRALLDGSSY"
     gene            complement(920..>1379)
                     /locus_tag="PLW80_08555"
     CDS             complement(920..>1379)
                     /locus_tag="PLW80_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66600.1"
                     /translation="GGLNPGALADKMAASVQDGVGRSLDGLRDTVRDYVARLVRQKEP
                     GATDEQIEALLDECLPDEGSDLSGTRAGSAADDLPPEAVAMMVRDFVDYSLGLMPPSR
                     QQELWEGIDAWQDKYWQAFSPELRAFVKARLEGRLAEDEFWSAVFSLLGL"
BASE COUNT          214 a          484 c          476 g          205 t
ORIGIN      
        1 tatacgacga gccgtcgagc agggcgcgga ggcgggtcgc gcaggctatc tggcccggct
       61 gcccgcgtac ctcgtgcacg cgcgggtcgt aggcgtcgac gataccgcgt agcgcctgcg
      121 aggtcagcgc cgccaccacg tcggcggtca gcgccagccg ctcggcgtcg taggcggcga
      181 gcgcggcgag ggcggtcatg cattgggtgc cgttgatgag cgcgaggccc tccttggcgg
      241 cgagctcgac cggtgccaga cctgcgccgg cgagcgcctc ggcggacgac atccgccttc
      301 ccttatagca acactccccc atgccgcaga gagctatggc gacatgggac aagggggcga
      361 ggtcgcccga cgcgccgagg gagcccttct cgggcaccac cggcgtcacg ccggcgttca
      421 gcatgtcgag cagggcctgg gcggtggaca gcctagcccc agagttgccc acggcgaggg
      481 cgttgacgcg caggagcatc atcgctcgga ccacctcctc ggggagcggg tcgccgacgc
      541 cgaccgcatg gctcatgatg aggttccgct ggagctcggc gttgctctcc cggctgatga
      601 cgacgtcgca gaactttccg aaaccggtcg ttataccgta gcgtatcagc gactcgtcga
      661 cgcagcgctc gacgacggcc ctggaggcct gcatcctcgg gatagcttcc cgcgccagtt
      721 cggccttcgc gccgaaacgg gccgccagcg cgacgtcgcc tatggcaagc cctcgtccgt
      781 caagcactat agtcttcatc gggaacctct tccgggcccc gcctcgcgcg ggaccgcgtc
      841 tcaagctgga tcgggccgca gtataccacg gggcagaggc cggggcgagg cgtcgaggcg
      901 attctatcga gcgcggcgct catagcccaa gcagactgaa cacggccgac cagaattcgt
      961 cctcggcgag tcgtccctcg agcctggcct tgacgaaggc ccgcagctcc ggggagaagg
     1021 cctgccagta cttatcctgc caggcgtcga tgccctccca tagctcctgc tgccgcgagg
     1081 gcggcatcag gccgagcgaa tagtcgacga agtccctgac catcatcgcg acggcctcgg
     1141 gcggcaggtc gtcggcggcc gagccggccc gcgtccccga caggtccgac ccctcgtcgg
     1201 gcaggcattc gtcgagcaga gcctcgatct gctcgtcggt cgcgccgggc tccttctggc
     1261 ggacgaggcg ggccacgtag tcgcgcacgg tgtcgcgcag cccgtcaagg ctgcgcccga
     1321 cgccgtcctg gaccgaggcg gccatcttat cagccagggc gccgggattg agaccgcca
//
LOCUS       DAOOLN010000664         1379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_126159_length_1379_cov_2.524169, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000664 DAOOLN010000000
VERSION     DAOOLN010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126159_length_1379_cov_2.524169"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PLW80_08560"
     CDS             complement(<1..342)
                     /locus_tag="PLW80_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66601.1"
                     /translation="MSIIEYVEAREILDSRGNPTIEVDVVLEDGTLGRAAVPSGASTG
                     EYEAVELRDGDKKRYLGKGVLKAVENVNNVIAGEICGLDCTEQVDIDRTMIDVHLLGA
                     VEAADLDCTEQV"
     gene            complement(477..>1379)
                     /locus_tag="PLW80_08565"
     CDS             complement(477..>1379)
                     /locus_tag="PLW80_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HPB66602.1"
                     /translation="RGAAPSLGETVLSVDAAARVVVTDRRELSFKAALVAPGRAGFAF
                     LQEVMRSNGVPFIDNVVDVGVRVELNEDRYPIVKDYYDPKFLFPKKVRTFCTNSRAAH
                     VVQERYEDEAGAWYSVNGHAWSKDRPANGLANFAMLKTIALTEPLASGQAYARALGAQ
                     AMLLGGGRPIMQRVGDFRLGRRSTREAFTGDLYDFAPTLPSCTPGDLGLAVPAKVMRA
                     LWNALKSLDTIAPGVLHPSTVLYYPEIKTYANKPVFQDPASFRAAPGLYLAGDGAGTS
                     RGITAAWASGMRVADGMLGELGER"
BASE COUNT          208 a          442 c          496 g          233 t
ORIGIN      
        1 cacctgctcg gtgcagtcga gatctgcggc ctcgactgca ccgagcaggt ggacatcgat
       61 catggtgcgg tcgatgtcca cctgctcggt gcagtcgagg ccgcagatct cgccggcgat
      121 gacgttgttg acgttctcga cggccttgag cacgcccttg cccaggtagc gcttcttgtc
      181 gccgtcgcgt agctccacgg cctcgtactc gccggtcgag gcgccggagg ggacagcggc
      241 gcggccgagg gtgccgtcct cgaggacgac gtccacctcg atggtgggat tgccgcggga
      301 atcgaggatc tcgcgggctt ctacgtattc gatgatgctc atgacgggct ccttaacgct
      361 gaggatgacg acgagccggc gagccggccc cgatgcagta ctatcgtgta ttctcgaaca
      421 gtaacttttt taggtaccac agtcaaggat agtcgcgagc ttgcatgcct ttgagtctat
      481 cgctcgccga gctcgccgag catcccgtcg gcgacgcgca taccagaggc ccaggcggcg
      541 gttattccgc ggctcgtgcc ggcgccgtcg cccgccaggt agaggccggg cgccgctcgg
      601 aagctggccg ggtcctggaa gaccggcttg ttggcgtagg tcttgatctc cgggtagtac
      661 agcactgtgc tcgggtgcag cacgcccggc gctatcgtgt ccaggctctt gagggcgttc
      721 cagagcgcgc gcatcacctt ggccggcacc gccagaccga ggtcgccggg cgtgcaggac
      781 ggcagcgtcg gcgcgaagtc gtacaggtcg cccgtgaagg cctcgcgggt ggagcgccgg
      841 ccgaggcgga agtcgccgac gcgctgcatg atcgggcgcc cgccgcccag gagcatcgcc
      901 tgggcgccga gcgcgcgcgc gtaggcctgg cccgaggcga gcggctcggt cagcgctatc
      961 gtcttgagca tggcgaagtt cgcgaggcca ttggccggcc ggtccttgga ccaggcgtgg
     1021 ccgttcaccg agtaccaggc gcccgcctcg tcctcgtacc gctcctgcac gacatgggcc
     1081 gcgcgagagt tggtgcagaa ggtacggacc ttcttgggga acaggaactt ggggtcgtag
     1141 tagtccttga cgatggggta gcggtcctcg ttcagctcga cgcggacgcc gacgtccacg
     1201 acgttgtcga tgaagggcac gccgttggag cgcatcacct cctgcaggaa ggcgaagccg
     1261 gcgcggcccg gggcgacgag ggccgccttg aacgacagct cgcggcggtc ggttacgacg
     1321 acccgggcgg ccgcgtcgac cgacaggacc gtctcgccga gcgacggggc ggcgccgcg
//
LOCUS       DAOOLN010000665         1378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_126273_length_1378_cov_3.053666, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000665 DAOOLN010000000
VERSION     DAOOLN010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126273_length_1378_cov_3.053666"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /gene="prs"
                     /locus_tag="PLW80_08570"
     CDS             <1..872
                     /gene="prs"
                     /locus_tag="PLW80_08570"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HPB66603.1"
                     /translation="ARKPQALILAPTRELALQVAEAFGKYSAHLPKVKVLPIYGGQSY
                     VVQLNGLKRGAQVIVGTPGRVIDHLDRGTLDISELEFLVLDEADEMLTMGFAEDVERI
                     LADTPDSKQVALFSATMPRAIARLAQQYLKDPKEITVKAKTATDSNITEMKLLGDIHG
                     KIVFMADDMLGTGGTLLKAMRYLKDQGATKVICAVSLPLFSANAIDDFDKAYREGLFY
                     RIIGTNAVHHGQLLKKEWYIEADVTTLFAQIISLLHHNRSLSPMLDNREIIAKKIKKA
                     MAAQAAHQPTLPL"
     gene            875..>1378
                     /locus_tag="PLW80_08575"
     CDS             875..>1378
                     /locus_tag="PLW80_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPB66604.1"
                     /translation="MRLYRVPERIDAFLFDIDGTLYTDREYGRFQIEVLVRELARIWG
                     EPYDEVAALVERTRAALADAEGRASTSLGNAMAALGVDIGTGVEWRRRLIRPERFLSV
                     DPALVAALSGLRDRYGSALVAVTNNPRSVGEATLEALGVHGLFSAVVGLDDTMRSKPA
                     AEPYLLAA"
BASE COUNT          250 a          480 c          422 g          226 t
ORIGIN      
        1 ccgcccgcaa accgcaggcg ttgatcctcg cgccgacacg tgagctcgcc ctgcaggtgg
       61 ccgaggcgtt cggcaaatat tcggcgcatc tgcccaaggt gaaggtgctg ccgatctacg
      121 gcgggcagag ttacgtcgtt cagctcaacg gtctcaagcg cggtgcgcag gtcatcgtcg
      181 gcacccccgg acgtgtaatc gaccatctcg atcgcggcac cctcgacatc tccgagctcg
      241 agttcctcgt gctcgacgag gccgacgaga tgctcacgat gggctttgcc gaggacgtcg
      301 aacgcatcct cgccgacacc ccggactcca agcaggtcgc gctgttctcg gcgaccatgc
      361 cgcgggccat cgccaggctg gcgcagcagt atctcaagga tccgaaggag atcacggtca
      421 aggccaagac cgcgaccgac tcgaacatca ccgagatgaa gctgctcggc gacatccacg
      481 gcaagatcgt gttcatggcc gacgatatgc tcggcaccgg tggcacgctg ctcaaggcta
      541 tgcgctacct caaggaccag ggcgccacga aggtgatctg cgccgtatcg ctaccgctgt
      601 tctcggcaaa cgcgatcgac gacttcgaca aggcctaccg cgagggtctg ttctaccgga
      661 taatcggaac gaacgccgtg caccacggcc agctcctcaa gaaggagtgg tacatcgagg
      721 ccgacgtgac cacgctgttc gcgcagatca tatcgctgct gcaccacaac cggagcctgt
      781 cgccgatgct cgacaatcgc gagatcatcg ccaagaagat caagaaggcg atggccgcgc
      841 aggccgccca ccagccgacg ctcccgctct gactttgagg ctctatcgcg ttcccgagcg
      901 tatcgacgcg ttcctcttcg atatcgacgg cacgctatat acggatcgcg agtacggccg
      961 ctttcagatc gaggtgctgg tgcgtgagct ggcccgcatc tggggcgagc catacgacga
     1021 ggtcgcggcc ctggtcgagc gtacgcgggc cgctctggcc gacgccgaag gccgcgcctc
     1081 caccagcctc ggcaacgcga tggccgccct cggggtcgac atagggactg gcgtcgagtg
     1141 gcggcggcgc ctgatacgcc ccgaacgctt cctgtcggtc gaccctgcgc tcgtcgccgc
     1201 cctctccggg ctccgcgacc gctacggctc ggcgctcgtc gccgtcacca acaaccccag
     1261 gtcggtagga gaggcgacgc tcgaggccct cggcgtccac ggcctattct cggctgtcgt
     1321 cggcctcgac gataccatgc gctccaagcc ggcggccgag ccctacctgc tagccgcc
//
LOCUS       DAOOLN010000666         1378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_126305_length_1378_cov_2.541194, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000666 DAOOLN010000000
VERSION     DAOOLN010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126305_length_1378_cov_2.541194"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /locus_tag="PLW80_08580"
     CDS             <1..1214
                     /locus_tag="PLW80_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HPB66605.1"
                     /translation="KLPFVDRVVARVIKDETKVISDKFGDDRRTEIVHDEVEAINIED
                     LITKEEMVIMLSNKGFVKRVPVSAYRSQSRGGKGSNSASLLDDDYVQQIFVANTHDYL
                     MYISSAGKAYWQKVHEIPEASRAARGGHLKSLLAIGANEEITTVIDLKGFSDDTYLML
                     GTLRGVVKRVATSQFVNAKTRGIIAIHLDEGDRLVSACRTRGNDEVVLITRLGQALRI
                     SERDVRVMGRGSRGVCGIKLADADELNAMLRVNPEEQMLLISETGYGKRTDYDNFNAH
                     GRATRGQRIYDPDEKSGEIVCAITVKENDDVVVMTSLGKTLKTRAADVRLCGKTARGV
                     HIVNIDSPDFVIGMDRIINEEAEIAVSKQAHDDDAKNRESEDEPVDTVAYDDEPEELP
                     PDADDGEVLDD"
BASE COUNT          317 a          407 c          426 g          228 t
ORIGIN      
        1 acaagctgcc cttcgtggac cgcgtggtgg cccgcgtgat caaggacgag accaaggtga
       61 tctccgacaa attcggcgac gaccgcagaa cggagatcgt ccacgacgag gtcgaggcta
      121 tcaatatcga ggacctgatc accaaggaag agatggtgat catgctgtcg aacaagggct
      181 tcgtgaagcg cgtgccagtc agcgcctatc ggagccagag ccgcggcggc aaaggctcga
      241 attcggcctc gctgctcgac gacgactacg tgcagcagat cttcgtcgcg aatactcacg
      301 actacctgat gtacatctcg agcgccggca aggcctactg gcagaaggtt cacgagattc
      361 cggaggcctc gcgagcggcc cgcggcggcc acctcaagag cctcctcgcg atcggggcga
      421 acgaggagat tacgaccgtc atcgatctca agggcttctc cgacgatacc tacctgatgc
      481 tcgggacgct gcgcggcgtc gtcaagcgcg tggcgaccag ccagttcgtc aacgcgaaga
      541 ccaggggcat catcgccatt cacctcgacg agggagaccg gcttgtatcc gcctgcagaa
      601 cgcgcggtaa cgacgaggtc gtgctcatta cccgtctcgg gcaggcgctt cgtatcagcg
      661 agcgcgatgt gcgtgtgatg ggccggggta gccgaggtgt ctgcggcatc aagctggcgg
      721 acgccgatga actgaacgcg atgctcaggg tcaatcccga agaacagatg ctcctgatca
      781 gcgagaccgg ttacggaaag cgcaccgact acgataactt caacgcccac gggagagcga
      841 cgcggggaca gaggatctac gatccggacg agaaatccgg agagatcgtc tgcgccatca
      901 ccgtcaagga gaacgacgac gtcgtcgtga tgaccagcct gggtaagacg ctcaaaacca
      961 gggccgccga cgtacgactc tgcggcaaga ccgcccgcgg cgtgcatatc gtcaatatcg
     1021 acagccccga cttcgtgatc ggcatggacc gcatcatcaa cgaagaggcc gagatagcgg
     1081 tctcgaagca ggctcatgac gatgacgcca agaaccgcga gtcggaggac gagccagttg
     1141 atacggtagc gtacgacgat gaacccgagg agctaccccc tgacgccgat gacggcgagg
     1201 tactggacga ttagaggctc ctgaaaagct tctgcctgac acggccgcga ttccatatgg
     1261 gatcgcggcc tttttaaatg gctagccctg gatgtttcac gtgaaacagg cgacgaagct
     1321 ctgtccccga gacgagcctc tgtccctctc gcgatgtttc acgtgaaacg ctagactt
//
LOCUS       DAOOLN010000667         1378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_126334_length_1378_cov_2.110355, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000667 DAOOLN010000000
VERSION     DAOOLN010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126334_length_1378_cov_2.110355"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="PLW80_08585"
     CDS             complement(<1..870)
                     /locus_tag="PLW80_08585"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPB66606.1"
                     /translation="MARLPGLVSTQSSARRGSANLLLTYDDDVADSASLAASAKLAAP
                     AGSFLWIESRAPDERVWELLVRGDDDETCRSMAAAAAERIAALPLALEVALNFKVSAP
                     AIVVKPDRDRSSAMGLPFSIVAGAMRRSVQAPVAYKRVGDTGDTDVRVGASRRVDEEA
                     LGSIVVASRGGATLEAASLVRIGREERIARIQRRDRRRSASLSIRTRRMDPEKAARET
                     MAALEGLITPPGYTLTFDRDAIEAARRLRRVTLSFVAAVALAYMAMAALSESFGLPLV
                     ALAAVPPSLAAPAL"
BASE COUNT          236 a          502 c          447 g          193 t
ORIGIN      
        1 cagggccggc gcggccagcg aaggaggcac cgcggcgagc gcgacgaggg gcagtccgaa
       61 cgactcgctc aacgccgcca tggccatgta ggccagcgcc accgccgcta caaaggacag
      121 cgtgacccgc ctcagccgcc tggcagcttc gatcgcgtct cggtcgaagg tcagcgtata
      181 gccgggaggc gtaatgagcc cctcgagcgc ggccatcgtc tcccgcgcgg ccttctccgg
      241 atccatgcgt ctcgtccgga tcgacaggga tgccgacctg cgacggtctc gtctctgtat
      301 cctggctatc cgctcctcgc ggccgattcg gaccagggac gcggcctcca gggtcgcgcc
      361 gccccgcgag gcgaccacga tcgagccgag cgcctcctcg tcgactcgac gggaagcgcc
      421 tacgcgaaca tcggtgtcgc ccgtatcgcc gacccgttta tacgcgaccg gcgcctggac
      481 cgatcgccgc atggctcctg ccactatcga gaacggcaac cccatggcgg acgacctatc
      541 gcggtcgggc ttgacgacga tagccggcgc cgagaccttg aaattcaggg cgacctcgag
      601 agccaggggt agcgccgcga tacgctccgc ggcggccgcc gccatcgagc gacaggtctc
      661 gtcatcgtcg ccgcgaacga gcagttccca gacccgttcg tcgggcgccc ggctctctat
      721 ccacaggaag ctccctgccg gcgcggccag cttcgcagac gccgcgaggc tggcactatc
      781 ggcgacgtcg tcatcgtacg tcaacaatag gttggctgag ccgcgcctgg cgctcgactg
      841 agtcgagacc aggccgggta gcctagccaa cgacgccgca tagtcggcaa gcctcgagtc
      901 gaccgcctcg agtggcaggc cctggtcgaa ctcgaggcgc gcgtataggt cgcgcccctc
      961 cgttcgggtt ccgacgtctt tcggagagat cgagatggcg acgacgccgg ccagcccaat
     1021 cgcggccgac caggcgagta cgagccgcgg cctctcagcg cataccgcga tagcggccgc
     1081 cagaagccga gccgactgcc ttctcgctcg cggagtggtt gggcggccaa ccctgagacg
     1141 gtgaagacgc cgtgccattt ctaccgatcg tcgcctcaac ccgaccatat cgccacccca
     1201 caggacgacg ggcggcatga tcgctatcgt cgccgcgtac gagacgccgc aggtgacggc
     1261 tatgcccgcc gctaccgcgc cgacgcccgg cgagacggcc tcgagtccga acatcggagc
     1321 cagcgccaag atagtggtgg cagtacccgc agccagggcc ggcgccagct cccggacc
//
LOCUS       DAOOLN010000668         1374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_126861_length_1374_cov_2.230478, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000668 DAOOLN010000000
VERSION     DAOOLN010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126861_length_1374_cov_2.230478"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="PLW80_08590"
     CDS             complement(<1..1197)
                     /locus_tag="PLW80_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66607.1"
                     /translation="MTDKEKKRFVYIGIAALLALAAIAAIVLALGAGGRSGERERTNM
                     LSLAGEYIDRGDFDRALDILDRLIIKDSSDAEARSLQDLALARKASAKAAAEEAAKAG
                     SGEAGALAQSLGELGKSLERTASTVAQSATRPSQPAAGSSAGSADDSAAAQAAARKAE
                     EDALAKAQSEAEAARRRAAEEELSRKSAELRAKMEAINALVKAGRQSISAGDYAAARS
                     SFDEAVSSLPSGEAKFSAQTYAEIAEGYYDGYRRDPTGAEGLESVKQAQRVAQEAIRS
                     DPTDAKPHYTLSKIYNDFAKPDLAQGELEQAVKLDPNNYLYAYELGKAYYRVRKFPEA
                     QRAFESITTKLNPKYEPAFFNLGMTYRAMRNDQAALTAFSGAIRLKPDYVRAHVESAV
                     ILAARGD"
BASE COUNT          185 a          484 c          437 g          268 t
ORIGIN      
        1 gtcacctcgg gctgccagga tgaccgcgct ttcgacgtgg gcgcgcacgt agtcgggttt
       61 cagcctgatg gctcccgaga acgccgtcaa ggccgcctgg tcgttcctca tagcccggta
      121 ggtcatacca aggttgaaga acgccggctc gtacttcggg ttcagcttgg tcgtgatcga
      181 ctcgaaggct cgctgagcct cggggaattt gcgtacccgg taataggcct tgccgagctc
      241 atacgcgtac aggtaattgt tcgggtcgag cttgaccgcc tgctcgagct ctccctgggc
      301 aaggtcgggc ttcgcgaagt cgttatagat cttgctgagc gtatagtgcg gcttggcgtc
      361 ggtcgggtcg ctccgtatgg cctcctgggc cacccgctgg gcctgcttga ccgactcgag
      421 gccctcggcg cccgtcgggt cgcgtctgta cccgtcgtag tagccctcgg ctatctcggc
      481 gtaggtctgg gcgctgaact tcgcctcgcc cgacggtagc gacgatacgg cctcgtcgaa
      541 ggaagagcgg gccgccgcgt agtctcctgc cgatatcgac tgacgccccg ccttgaccag
      601 cgcgttgatc gcttccatct tggcgcgtag ctcggcgctc ttgcgcgaca gctcctcttc
      661 ggcggccctg cgcctagcgg cctcggcctc cgactgggcc ttggccaacg cgtcctcctc
      721 ggccttcctc gcggccgcct gtgccgcggc cgaatcgtcg gcggaacccg cactggagcc
      781 ggcagccggc tgagacggcc tcgtcgcgct ctgggccacg gtgctggccg tgcgctcgag
      841 cgacttgcct agctcgccga ggctctgcgc cagagcgccc gcctcgccgg aaccggcctt
      901 cgccgcctcc tcggcggccg ccttggccga ggccttacga gccaaggcca ggtcctggag
      961 cgatcgcgcc tcggcgtcgg acgagtcctt gatgatcagc cgatccagga tatcgagcgc
     1021 ccggtcgaaa tcgccccgat caatatactc gccggccagg ctgagcatgt tcgtccgttc
     1081 ccgttcgccg gagcggcctc ccgccccgag cgccaggacg atggccgcga tcgccgccaa
     1141 ggcgagcagg gccgctatcc cgatatagac gaaccgcttc ttctctttat cagtcacgat
     1201 gcttcccttc gtttcggcgt cgccagcggg cgatggccgt caatcgtcga gcgacaggtc
     1261 ggattcatag ccttgcacgt cgcccgagcc ggcggacagg ttcgtcgtcg cctcggcggc
     1321 ctcgagcagc gacctggcca cctcgtcgag caaggccttc ctcgcctgct cctc
//
LOCUS       DAOOLN010000669         1372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_127168_length_1372_cov_2.262718, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000669 DAOOLN010000000
VERSION     DAOOLN010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127168_length_1372_cov_2.262718"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1372
                     /gene="topA"
                     /locus_tag="PLW80_08595"
     CDS             <1..>1372
                     /gene="topA"
                     /locus_tag="PLW80_08595"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPB66608.1"
                     /translation="KKSKAVLLASDNDREGEAISFHIRAALAQKNEGLDIKRIVFNEI
                     TPQAIRDAVKKPSALDEDKVNAQKARRVLDRLVGYNLSPLLWKKVKNGLSAGRVQSVA
                     LRLICEREVEVESFIPEEYWSLEADFKKGRYAFSGELVSYDGAKAELKGESDVEAITR
                     AIEGLPCLVSDVKESERQVKPKPPFTTSKLQQTAANRLGFTSKKTMQIAQQLYEGINI
                     GSTRIGLITYMRTDSVRVSDVALKEVRDWLGERFPAELPAEPVVYATDKKAQDAHEAI
                     RPTMVAYTPDAIKEYLNKDQQKLYGIIWERFVASQMTQAKQRSTSMEVSVGLARFRVG
                     ASRLVEKGFYKVIKLLATKDDKESTLPEMRIGEELKLEGYRPEQHFTQGPARFTDASI
                     IKLLEEKGIGRPSTYAPIISVLLERYYVTRENKQLVPTTLGRKINEILVGSFGDVVDA
                     GFTADME"
BASE COUNT          291 a          436 c          430 g          215 t
ORIGIN      
        1 caagaaatcg aaggcggtgc tgctcgcctc cgataacgat cgcgagggag aggccatctc
       61 cttccatatc cgagccgccc tcgcccagaa gaacgagggc ctcgacataa agcgcattgt
      121 cttcaatgag ataaccccgc aggccatacg tgacgcggtc aagaagccgt cggccctcga
      181 cgaggacaag gtgaacgccc agaaggccag gcgcgtgctc gaccgtctcg tcggctacaa
      241 cctgtccccg ctactctgga agaaggtcaa gaacggcctc tcggccggcc gcgtccagtc
      301 ggtcgccctg cgcctgatct gcgagcgcga ggtcgaggtg gagtcgttca tccccgagga
      361 gtactggtcg ctcgaggcgg acttcaagaa ggggcgctac gcgttctcgg gcgagctcgt
      421 ctcatacgac ggagcgaagg ccgagctcaa gggcgagtcc gacgtcgagg ccatcactcg
      481 cgccatcgaa gggctgccct gcctggtctc cgacgtaaag gaaagcgagc gccaggtcaa
      541 gccgaagccg cccttcacga cctccaagct gcagcagacc gcggccaata ggctcggctt
      601 caccagcaag aagacgatgc agatagccca gcagctgtac gaaggcatca acatcggctc
      661 gacccgcatc ggcctcatta cctatatgcg taccgactcg gtgcgcgtct cggacgtggc
      721 gctcaaggaa gtgcgcgatt ggctcggcga gcgtttcccc gccgagctgc ccgccgagcc
      781 cgtcgtctac gccaccgata agaaggccca ggacgcgcac gaggcgatcc ggccgacgat
      841 ggtcgcctat acgccggatg cgatcaagga gtacctgaac aaggaccagc agaagctgta
      901 cggcataatc tgggagcgct tcgtcgccag ccagatgacc caggccaagc agcgctcgac
      961 ctcgatggaa gtctccgtcg gcctggccag gttccgcgtc ggcgcctcgc ggctagtgga
     1021 gaagggcttc tacaaggtca taaagctgct cgcgacgaag gacgacaagg agtcgaccct
     1081 gccggagatg cgtatcggcg aggagctcaa gctcgagggc tatcggccgg agcagcactt
     1141 cacgcagggg ccggcccgct ttaccgatgc ttcgatcatc aagttgctcg aggagaaggg
     1201 catcggccgc ccgtccacct atgcgccgat catatcggta ctgctcgaac ggtactatgt
     1261 gacccgggag aacaagcagc tggtgccgac cactctgggc cgtaagataa acgagatact
     1321 cgtcggctcg ttcggcgacg tggtcgacgc cggcttcacg gccgacatgg ag
//
LOCUS       DAOOLN010000670         1371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_127242_length_1371_cov_3.946809, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000670 DAOOLN010000000
VERSION     DAOOLN010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127242_length_1371_cov_3.946809"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PLW80_08600"
     CDS             complement(<1..475)
                     /locus_tag="PLW80_08600"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB66609.1"
                     /translation="MPSKASESEPVRFDEPGWFGDDGFWTDYAPLLFGGERWAEAPAV
                     VDAVLALSGAEPGSPVLDMCCGPGRHALEFASRGHPVVGVDITEPYLEAARDSAEAMG
                     LTAEFVRGDARSWCRPGAFSLAVNLYTSFGYFGSKAEDEAMLARLAASLAPGGALV"
     gene            complement(465..842)
                     /locus_tag="PLW80_08605"
     CDS             complement(465..842)
                     /locus_tag="PLW80_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66610.1"
                     /translation="MQAIDRLKSDLDDWVRRSGRAPGNREFDSLRDTFARSVPKVPGE
                     TMAALFTTFLSRYGSGGSEYSAKAIDWLAGVGSLLLSDYDGTPFTREDWEEIRDIVTV
                     ESGEIDMDTLSYVLGQALEHGAV"
     gene            complement(1059..>1371)
                     /locus_tag="PLW80_08610"
     CDS             complement(1059..>1371)
                     /locus_tag="PLW80_08610"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HPB66611.1"
                     /translation="IVALSAGFAISVAYREELFYRCYLLGALTGRGAGAVAAAAVSTG
                     LFVAGHAYQGPAGMLASAIAGSALAAARLRGARLHALAWGHAAYDLAVLIAGFGLAAR
                     A"
BASE COUNT          230 a          455 c          461 g          225 t
ORIGIN      
        1 tgacgagcgc gccgccgggc gcgaggcttg cggcgagcct cgcgagcatc gcctcgtcct
       61 cggccttcga gccgaagtag ccgaagctgg tgtacaggtt gacggcgagc gagaacgcgc
      121 ctggccggca ccatgatcgg gcgtcgccgc gtacgaattc ggccgttagc cccatcgcct
      181 cggcgctgtc ccgggccgcc tccaggtacg gctcggtgat gtcgacgccg accaccgggt
      241 ggccgcgcga ggcgaattcc agcgcatgcc taccaggacc gcagcacatg tcgagtaccg
      301 gcgacccggg ctcggccccg ctcagggcca ggacggcgtc gacgacggcc ggcgcctccg
      361 cccagcgctc gcctccgaac agcaggggcg cgtagtcggt ccagaagccg tcgtctccaa
      421 accatcctgg ttcatcgaac cgtaccggtt cggattctga agccttagac ggcaccgtgc
      481 tcgagcgcct gtccgagcac gtagctcaag gtgtccatgt ctatctcgcc ggactctacg
      541 gtgacgatat cgcgtatttc ctcccagtcc tcccgggtga acggcgtgcc gtcgtagtcg
      601 gacaggagta gcgagccgac tccggccagc caatcgatgg ccttggccga gtattccgag
      661 ccgcccgagc cgtagcggga caggaaggtg gtgaagaggg cggccatcgt ctcgccgggc
      721 accttgggaa ccgatcgcgc gaaggtgtcg cgtagcgagt cgaactcgcg gttgcccgga
      781 gctcggccgg atcggcgaac ccagtcgtcc aggtcggact ttaagcggtc tattgcttgc
      841 atcaccctat aatacagtct gccgtgctac ttgggtagtg ttacgaacct tgttgatggg
      901 ccgtacgcgt gtttgagtgt tacgaatctt cgtggtatcg cagttccgac agatgacgac
      961 ttcccaataa agccgttcgc cgaggtggaa tcgctgctag cgggccagcg gcgcggctga
     1021 tacgcgctct ttgcgtgtcc cccataagat tcgtaacact atgcgcgggc cgcgaggccg
     1081 aacccggcga tcaatacggc gagatcgtag gccgcgtggc cccatgccag cgcgtggagt
     1141 ctggcgcctc gtagcctggc ggccgccaag gccgagcccg cgatggccga ggccagcatc
     1201 ccggccggcc cctggtaggc gtgcccggct acgaacagcc ccgtcgatac ggcggccgcg
     1261 gcgacggccc cggcgccgcg gccggtcagc gcgccgagca ggtagcagcg gtagaacaac
     1321 tcctcgcggt aggccaccga gatcgcgaag ccggccgata gggcgacgat g
//
LOCUS       DAOOLN010000671         1371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_127332_length_1371_cov_2.046353, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000671 DAOOLN010000000
VERSION     DAOOLN010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127332_length_1371_cov_2.046353"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1371)
                     /locus_tag="PLW80_08615"
     CDS             complement(<1..>1371)
                     /locus_tag="PLW80_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB66612.1"
                     /translation="IVFGAVATASFIERDFGAVAVSRVRIPVRTNDGLDAYIPGKLYV
                     PALASEANPVPAVLALHGYQNDKDTSAAFALELARRGIAALAIDQYGHGESPLGLRHR
                     GYDRTKGGPARFKLFMSFSALNGAGVDGLVDSSMGATAAFRWLRDRDYVRGDKVGVTG
                     HSMGTWSAVTVARENPEHAAIVLQCGQPYGPVYGADGSVELKNYLMLQARYDEFDYFR
                     DYELTTGRLNEAALRYGVFAGQDSPIAWNTTYGRFDDGSARRMEMIETVHRGVTHSSR
                     AISAAMAWFTEALGVEPTIAPDDLVFMARELLVGLALLVGALSLMPLGSLLLATPFFA
                     AVAQPMPERYVMPSRSWRRSAAISVTLSAALYPFMTQLGHGLFPYPDGLFKTLMAGGL
                     ILWLDTLAVIAFFSLRAWYRHGEGRRLGVTMYDLGLSFDREKTVIDWGIVGKTALMAL
                     AMFSYLL"
BASE COUNT          257 a          453 c          450 g          211 t
ORIGIN      
        1 caggaggtag ctgaacatcg cgagggccat cagcgccgtc ttgccgacga taccccaatc
       61 gatgacagtc ttctcccggt cgaacgacag gcccaggtcg tacatcgtca cgccgagtcg
      121 ccggccctcg ccatgcctat accaggcgcg tagtgaaaag aacgcgatga cggccagggt
      181 atccagccag aggatcaggc cgccggccat cagagtcttg aagaggccgt ccggataggg
      241 gaacagccca tggcccagct gcgtcatgaa cgggtagagg gccgcgctca gggtgacgct
      301 gatcgcggcg gagcgacgcc agctacgaga cggcatcacg tagcgctcgg gcatcggctg
      361 ggccaccgcg gcgaagaacg gcgtggccag gagtagcgag cccagcggca tcagggacag
      421 cgctccgacg agcaaggcca ggccgacgag cagctcccgc gccatgaaga cgaggtcgtc
      481 gggagctatg gtcggttcga cgccgagcgc ctcggtgaac caggccatcg ccgcggagat
      541 agcacgcgag ctatgggtca cgccccggtg cacggtctct atcatctcca tgcgcctggc
      601 gctaccatcg tcgaagcggc cgtaggtagt attccaggca atgggcgagt cctgaccggc
      661 gaagacgccg tagcgtagcg ctgcttcgtt cagcctcccc gtcgtcagct cgtagtccct
      721 gaagtagtcg aattcgtcgt agcgggcctg gagcatcagg tagttcttga gctcgaccga
      781 tccgtccgcc ccgtagaccg ggccgtacgg ctggccgcac tggagaacga tggccgcgtg
      841 ctcggggttc tccctagcga ccgtgaccgc gctccaggta cccatagagt gcccggtaac
      901 ccctactttg tcgccgcgca cgtagtcccg gtcgcgtaac cagcggaaag ccgcggtggc
      961 gcccatcgac gagtcgacga gcccgtcgac gccggccccg ttcagcgccg agaagctcat
     1021 gaacagcttg aaacgcgccg gaccgccctt cgtccgatcg tagccccgat gcctcaggcc
     1081 gagcggactc tcgccgtggc catactggtc tatcgcgaga gccgcgatgc cgcgcctggc
     1141 cagctcgagc gcgaacgcgg cggaggtatc cttatcgttc tgatagccgt gtagcgccag
     1201 cacggcgggt acaggattcg cctcactggc taacgcggga acgtagagct tgcccggaat
     1261 ataggcgtcg agcccgtcgt tcgtccgtac ggggatgcgt acgcgcgaca cggccaccgc
     1321 gccgaagtcc cgttcgatga acgacgccgt cgcgacggcc ccgaagacga t
//
LOCUS       DAOOLN010000672         1369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_127610_length_1369_cov_2.023592, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000672 DAOOLN010000000
VERSION     DAOOLN010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127610_length_1369_cov_2.023592"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1369)
                     /locus_tag="PLW80_08620"
     CDS             complement(<1..>1369)
                     /locus_tag="PLW80_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66613.1"
                     /translation="GDYRYGDASIEAELRFGGPGPKRAAGLLLRKLDDSSFLYTLVSS
                     DGEARLDLVFNGEPRALVPWVACPWAAGADEMVLSVVLRGPRVVVLVNGRFALEAEDD
                     SLDLGGVAFGAQSYDGPATIELRSVRVESRPLEAEADYLRFARVVAAAPDQRRRLAEG
                     LFSLGYYVPALVQLRKIADGSGATAADRFLEAECLLRLELYDEAAKAVDACLALEPGM
                     DNALEERFNLLYLRGDYVGLRDALASDLERVGRSPRLANLLGHAHYNLGAWAEAAEAY
                     GAAAAGDPDMPIYSRNGAAALEKTGDLVAAAARWLAAARGFYDQGAWDDAEDCSRRLR
                     GLGYDAASLDSLDGLVAYGRGDEKTAAAILGKLWKRGKADAPAAYVYGLIAAGSGERG
                     GAIKAFRRATELEPGRAIYRYRLAESLFMAGEPFGSELAAAIEAAPDDGWTLNLAGQA
                     ALRAGD"
BASE COUNT          195 a          496 c          488 g          190 t
ORIGIN      
        1 gtcgccggcc ctcagggctg cctgcccggc caggttgagc gtccagccgt cgtccggcgc
       61 ggcctcgatc gccgcggcca gctcggaacc gaagggttcg cccgccatga acagcgactc
      121 ggccagccta taccgataga tggctcgccc cggctcgagc tccgtggcgc gcctgaacgc
      181 cttgatcgcg ccgccgcgct ctcccgagcc ggcggctatc agtccgtaga cgtaggcggc
      241 gggggcgtca gccttgccgc gtttccacag cttgcccagg atagcggcgg cggtcttctc
      301 gtcaccccgg ccataggcta cgaggccgtc gagcgagtcc aggctcgcgg cgtcatagcc
      361 gaggccgcgt agccgcctcg aacagtcctc ggcgtcgtcc caggctccct ggtcgtagaa
      421 gccgcgggcg gcggcgagcc agcgggcggc ggcggccacg aggtcgcccg tcttctcgag
      481 cgccgccgcg ccgttgcgcg agtagatcgg catgtcgggg tccccggcgg ccgcggcacc
      541 gtaggcctcg gccgcctcgg cccaggcgcc gaggttgtag tgggcgtggc cgagcaggtt
      601 cgcgagccgg gggctacgcc cgactcgctc cagatcggag gccagcgcgt cgcgaaggcc
      661 aacgtagtcg ccgcgcaggt agagcaggtt gaaacgttcc tcgagcgcgt tatccatgcc
      721 cggttcgagc gccaggcacg cgtcgacggc cttggccgcc tcgtcgtaca gctcgaggcg
      781 caagaggcat tccgcctcca ggaacctgtc ggcagccgta gcccccgagc cgtcggctat
      841 cttgcgcagc tgcacgaggg ccggcacgta gtagccgagg gagaataggc cctccgccag
      901 gcgccgcctc tggtcgggcg cggcggccac cacccgcgcg aagcgaaggt agtcggcttc
      961 cgcctcgagc gggcgcgact cgacgcgcac ggagcgtagc tcgatcgtcg ccggtccgtc
     1021 gtagctctgc gcgccgaagg ccacgcctcc caggtcgagc gagtcgtcct ccgcctcgag
     1081 cgcgaaacgg ccgttgacga ggactacgac gcgcggaccg cgaagtacga cgctgagcac
     1141 catctcgtcc gcgccggcgg cccaggggca ggctacccag ggtacgaggg cccgcggctc
     1201 gccgttgaac accaggtcga gccgtgcctc gccatcgctc gatacgagcg tgtacaggaa
     1261 ggacgagtcg tcgagcttgc gtaggaggag gccggccgcc cgcttggggc ccgggccgcc
     1321 gaagcgtagc tccgcctcga tcgacgcgtc gccgtagcgg taatccccg
//
LOCUS       DAOOLN010000673         1366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_128022_length_1366_cov_3.025172, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000673 DAOOLN010000000
VERSION     DAOOLN010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128022_length_1366_cov_3.025172"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PLW80_08625"
     CDS             <1..402
                     /locus_tag="PLW80_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPB66614.1"
                     /translation="REIAKMLDMDASHVADLVNVSRDLVSLEAPVFDERDSSVLGDFV
                     ENDRYQSPDDHVIEQGLKDDINDVLRTLTEKESEVLQYRFGLNGRRAMSLKEIGDRFN
                     LTKERIRQIEKAALKRLSVPGRTESLEGYVA"
     gene            540..1244
                     /locus_tag="PLW80_08630"
     CDS             540..1244
                     /locus_tag="PLW80_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HPB66615.1"
                     /translation="MDQLLTWLEGPVVDFNAALARLIFSLFACGLIGLEREMRRQTAG
                     WRTHIVIGLGATLLMLLSIWLPQSIGGDKGDPGRIAAQVVSGIGFLGAGAFVKIGNNV
                     KGLTTAATIWFVAGLGLAIGAGMWEIALIVLVLSLLVLTFLEPLEKKWFPSERLKLLQ
                     IWYEGSSLDRKSVYATLKTFRINVQSVDASLSVQKKQTRLSALVKVPVNVDLDTLFTE
                     LRKTGKVAKVRLHENY"
BASE COUNT          237 a          470 c          430 g          229 t
ORIGIN      
        1 cgcgagatag ccaagatgct ggacatggac gcaagccacg tcgccgacct cgtcaacgtc
       61 tcccgcgatc tcgtctcgct cgaggctccg gtcttcgacg agcgcgactc gagcgtgctc
      121 ggcgacttcg tcgagaacga tcgctaccag tctccggacg atcacgtgat cgagcagggg
      181 ctcaaggacg acatcaacga cgtgctccgg acgcttaccg agaaggaaag cgaggtgctc
      241 cagtaccgct tcgggcttaa cgggcgccgc gccatgagcc ttaaggagat cggcgaccgc
      301 ttcaacctga ccaaggagcg catccgccag atcgagaagg ccgccctcaa gcgcctgtcc
      361 gtgcccggcc gcaccgagtc gctcgaaggc tacgtagcct gatccgtccg cgccccaggg
      421 cgcgttcacg atatagcggc cgcccggaga tgcctccccg ggcggccgcg ctttattcgc
      481 ggccacggct cgaccctcct cgccgcgcat gctagaatag tgcggcaagg agcggcatcg
      541 tggatcaact gcttacctgg ctcgagggtc cggtcgtcga cttcaacgcg gccctggccc
      601 gactcatatt tagcctgttc gcctgcggcc tgatcggcct cgagcgcgag atgcgccgcc
      661 agacggccgg ctggcgcacg cacatcgtca tcggcctcgg cgctaccctg ctcatgctac
      721 tgtcgatctg gctgccccag tccatcggcg gcgacaaggg cgacccggga cgtatagcgg
      781 cccaggtcgt atcgggcatc ggcttcctcg gcgctggcgc cttcgtcaag ataggcaaca
      841 acgtcaaggg cctgaccacg gctgccacga tctggttcgt agcggggctc ggcttagcca
      901 taggcgccgg catgtgggag atagccctga tcgtgctcgt cctgtcgctc ctcgtactca
      961 ccttcctcga gccgctcgag aagaagtggt tcccgtcgga gcggctcaag ctgctgcaga
     1021 tctggtacga ggggagctcg ctcgaccgga aatcggtcta cgccacgctc aagacgttcc
     1081 gcatcaacgt ccagtccgtc gacgcgtcgc tgtcggtcca gaagaagcag acgaggctgt
     1141 cggccctggt caaggtgccg gtcaacgtcg acctggacac gctgttcacc gagctgcgca
     1201 agaccggcaa ggtggccaag gtcaggctcc acgagaacta ctaggtcgac tatagggccc
     1261 ttccgagacc cgggcgggtc ggggcccgcc cggccccgcg gctactccgc gggccggacc
     1321 gacgcgagct ccgccttcag ccgcaggtac tccgtgttca gccgcg
//
LOCUS       DAOOLN010000674         1366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_128054_length_1366_cov_2.601068, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000674 DAOOLN010000000
VERSION     DAOOLN010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128054_length_1366_cov_2.601068"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLW80_08635"
     CDS             <1..310
                     /locus_tag="PLW80_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66616.1"
                     /translation="TVDLDRENPSFKVDRLVQPAELKEKSYRTMHIALHQVRREDDLE
                     GLRDLLFGSSGQCGVVLHVRGGDDEVAVRAHAQISCSPNPDVVERIRETAIVSEVWLD
                     "
     gene            complement(307..1008)
                     /locus_tag="PLW80_08640"
     CDS             complement(307..1008)
                     /locus_tag="PLW80_08640"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPB66617.1"
                     /translation="MPIRAVGFDIDGTIYEASRLYAKLLVKGFGKIRFLLAFNAVRHE
                     LRDLLSSEEYRALGIRGVEAFHRYQAGLVARRLGRGADHIYEKIEDFFYTATVKPFAS
                     IPTYPGVADLLDELRAGGLKLGALSDFPCERKIGLLGLAGKFDVAMTSEETGLVKPDR
                     ASFDLLAERLGVRNDELLYVGNSERYDVLGALGAGMRAALITKDAKAAAATKAEFSFF
                     DYADLGRYLAERAAG"
     gene            complement(1100..>1366)
                     /locus_tag="PLW80_08645"
     CDS             complement(1100..>1366)
                     /locus_tag="PLW80_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HPB66618.1"
                     /translation="AAGTSPYAFVEIMTCPGGCLGGGGQPIAPTWEKRERRKEAIYAE
                     DRGMPLRKSHENESVKALYAEFLEKPLGHESHRLLHTKYAEKRA"
BASE COUNT          223 a          428 c          490 g          225 t
ORIGIN      
        1 caccgtcgac ctggaccgcg agaacccgag cttcaaggtc gaccgcctcg tccagccggc
       61 cgagctcaag gagaagagct accggaccat gcacatcgcc ctccatcagg tacgccggga
      121 ggacgacctc gaggggctgc gcgacctgct gttcggctcg agcgggcagt gcggcgtcgt
      181 gctgcacgtc cggggcgggg acgacgaggt ggcggtcagg gcgcacgcgc agatcagctg
      241 ctcgccgaac cccgacgtgg tggagcggat ccgggagacg gcgatcgtct ccgaggtctg
      301 gctcgactag ccggcggcgc gctcggccag gtagcggccc aggtcggcgt agtcgaagaa
      361 cgagaattcg gccttggtgg ccgcggccgc cttggcgtcc ttggtgatca gggcggcgcg
      421 catgcccgcg ccgagcgcgc cgagcacgtc gtagcgctcg gaattgccga cgtagagtag
      481 ctcgtcgttg cgcacgccga gccgctcggc gagcaagtcg aacgaggcgc ggtccggctt
      541 gacgagcccg gtctcctcgc tggtcatcgc cacgtcgaac tttccggcca ggccgaggag
      601 ccctatcttg cgctcgcagg ggaagtcgga cagagcgccc agcttgaggc ccccggcccg
      661 cagctcgtcg aggaggtccg ccacgccggg gtaggtcggt atcgaggcga agggcttgac
      721 ggtagccgta tagaagaagt cctctatctt ctcgtagata tggtcggcac cgcggccgag
      781 gcgcctggcg acgaggccgg cctgatagcg gtggaacgct tccacgccgc gtatgccgag
      841 ggcgcggtac tcctccgacg ataggaggtc gcgaagctcg tggcgcaccg cgttgaacgc
      901 gaggaggaag cggatcttgc cgaatccctt gacgaggagc ttggcgtaga gcctggaagc
      961 ctcgtagatg gtaccgtcga tgtcgaatcc aactgctcga atgggcatgg acacgttata
     1021 cagggtatgg ccactcctgt ctatgtaata cggccggccg cccgagatgg acggccggcc
     1081 cggaactagc ggagcgggct taggcccgct tctccgcgta cttcgtatgc aggagcctat
     1141 gcgactcgtg gccgagcggc ttctcgagga actcggcgta cagagccttg acggactcgt
     1201 tctcgtggga ctttcgcagc ggcatgccgc gatcctcggc gtagatggct tctttacgcc
     1261 gttcacgctt ttcccaggtc ggggcgatcg gctggccgcc gcctccgagg cagccgccgg
     1321 ggcaggtcat gatctcgacg aaggcgtagg ggctggtgcc ggcggc
//
LOCUS       DAOOLN010000675         1366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_128070_length_1366_cov_2.160183, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000675 DAOOLN010000000
VERSION     DAOOLN010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128070_length_1366_cov_2.160183"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /locus_tag="PLW80_08650"
     CDS             complement(<1..1287)
                     /locus_tag="PLW80_08650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB66619.1"
                     /translation="MDIHLQGAMDGLEAAAILLERWAIPVILLTAYADGPTIERAKLT
                     HPYAYILKPYDERELRTAISIGLYRASMEKRLRTSEERYRGLFEHGLAATMLAEPGGR
                     VLEANNAFAALAPDAGNAQDILADPVDAERLVAAMEKGDSYGPVEVRIKRADGAEAWV
                     LLSAAPMRLSDGTAAYQFQAVDITERKSLADQLLHAQKLSALGRFAGGVAHDFNNVLT
                     AVMGYSRLLRSDLEEEGHPLEELDGIDQAAKRAAALARQLLLFSRREDMKLAVFSLSE
                     LAREDERMLRRIIGEGATLVIRALADDDLVRVDRARVEQAVVNLIANARDVTDRGGRI
                     YLATGTVRYDEPVMGAIELVPAGDWSYIEVVDEGPGIPPELLDKVFQPFFTTKTADRG
                     TGLGLSTVAGIVRQAEGRIALVSKPGAGTTMRLLFPR"
BASE COUNT          212 a          464 c          457 g          233 t
ORIGIN      
        1 cctggggaat aggaggcgca tcgtcgtacc ggcgcccggc ttggagacca gggctatccg
       61 cccctcggcc tggcggacga tgccggcgac ggtggacaag ccgagacccg tgccccgatc
      121 cgcggtcttc gtggtgaaga acggctgaaa caccttatcc agtagctccg gcgggatgcc
      181 cggcccctcg tcgacgacct cgatgtacga ccagtcgccg gccggcacca gctcgatggc
      241 gcccatgacc ggctcgtcgt accggacggt tccggtcgcg aggtagatgc gtccgccccg
      301 gtcggtaacg tccctggcgt tggctatcag gttgacgacc gcctgctcga cgcgggcgcg
      361 gtcaacgcga accaggtcgt cgtcggcgag tgcccggatg acgagcgtgg ctccctctcc
      421 gatgatgcgc ctgagcatac gctcgtcctc gcgagccaac tccgacaggc tgaacaccgc
      481 cagcttcata tcctcccgcc tcgagaacag gaggagctgc ctggcgaggg ccgctgcccg
      541 tttcgcggcc tggtcgatgc catcgagctc ctcgagcgga tggccctcct cctcgaggtc
      601 gcttcggagc agcctcgaat agcccatgac cgcggtgagc acgttgttga agtcgtgggc
      661 gacaccgccg gcgaagcgcc cgagcgccga tagcttctgg gcgtggagga gctggtccgc
      721 cagggacttc cgctccgtga tgtcgaccgc ctggaactga tacgccgcag tgccgtcgga
      781 cagccgcatc ggcgcggcgc tgagcagcac ccaggcttca gcgccgtcgg ctcgtttgat
      841 acgcacctcg accggcccat acgagtcgcc cttttccatc gcggcgacca agcgttcggc
      901 gtcgactgga tcggccagga tatcctgcgc gtttcctgcg tcgggagcca aggccgcgaa
      961 cgcattgttc gcctccagta cgcggccacc cggctccgcg agcatcgtcg ctgccaggcc
     1021 atgctcgaaa agcccccgat agcgttcctc gctcgtcctg aggcgtttct ccatcgaggc
     1081 ccggtacaaa cctatcgaga tggccgtccg cagctcgcgc tcgtcatagg gcttgaggat
     1141 gtaggcgtag ggatgggtca gtttggcccg ttcgatcgtc gggccgtcgg cgtaggcggt
     1201 gaggaggatg accgggatgg cccagcgctc gagcaggatg gcggcggcct cgaggccgtc
     1261 catagcgccc tgcaggtgga tatccatcag cagggcgcta tggacggcat cgaggccgcc
     1321 gccatcctgc tcgagcgctg gatggcggcg gcctcgatgc cgtcca
//
LOCUS       DAOOLN010000676         1365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_128173_length_1365_cov_2.732061, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000676 DAOOLN010000000
VERSION     DAOOLN010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128173_length_1365_cov_2.732061"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PLW80_08655"
     CDS             complement(<1..655)
                     /locus_tag="PLW80_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPB66620.1"
                     /translation="MEKSIRFNILALVGPETAGVVRRAVDEVGGCDLRVDAGESALVA
                     VESGDQVDLVLLDPDAVSMATADGIAVLKAACPSCEIALLLGDHDITMDVELMRLGAY
                     DSLPNPPGVDSLALLIRGLMEKNETERENRAMVEAMGQGRSQTIISHSPEMEKVLSMA
                     ARAAKSDSTVLITGESGTGKELVASAIHLAGPRKHKAFVPVNIAALQENLIESELFGH
                     "
     gene            complement(655..>1365)
                     /locus_tag="PLW80_08660"
     CDS             complement(655..>1365)
                     /locus_tag="PLW80_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB66621.1"
                     /translation="WFAYKGEDWVLRDLSFTVEPGETLAIVGYTGAGKSTVGSLLARL
                     WDPQRGEIRLDGVPIRELPLDALRSSIRPVMQDVFLFSGTVAGNIDLGLGLGADDIRR
                     AAEAVRAAGFIERLEGGYGATVGEGAATLSSGQRQLLSFARVVAHDPAIVVLDEATSS
                     VDTETEQLLQEGLAAMMRGRTTVAIAHRLSTIRHADRIIVLADGRLAEEGDHDSLIAK
                     GGIYYNLYRLQYQGEASA"
BASE COUNT          214 a          478 c          441 g          232 t
ORIGIN      
        1 cgtggccgaa cagctcgctc tcgatcaggt tctcctgcag ggccgcgatg ttcaccggga
       61 cgaaggcctt gtgcttgcgc gggccggcca ggtgaatagc gctggctacc agctccttgc
      121 cggtgccgct ctccccggtg atcagcaccg tcgagtcgct cttggcggcc ctggcggcca
      181 tcgacagcac cttctccatc tcggggctat gggagatgat cgtctgggaa cggccttgcc
      241 ccatcgcctc gaccatggcg cggttctcgc gctcggtctc gttcttctcc atgaggccgc
      301 gtatcaaaag ggccagggaa tcgacgcccg gcgggttcgg caacgaatcg taggcgccga
      361 gccgcatcag ctccacgtcc atggtaatgt cgtggtcgcc gagtagcaag gcgatctcgc
      421 aactcgggca ggccgccttg agcacggcga tgccgtcggc cgtggccatc gagacggcgt
      481 ccgggtcgag caacacgagg tccacctgat cgccggactc gacggcgacg agggccgatt
      541 cgccggcgtc gacgcgcagg tcgcagcccc ccacctcgtc gaccgctcgc ctcacgacgc
      601 ccgcggtctc tggaccgacc agagccagga tattgaatcg gatacttttt tccattaggc
      661 ggaagcctcc ccttggtatt gcaggcggta caggttgtag tatatgccgc ctttggcgat
      721 aagactatcg tgatcgcctt cctcggccag cctgccgtcc gcgagcacta tgatgcggtc
      781 ggcgtggcgt atggtcgaca ggcggtgggc tatggcgacg gtcgtccggc cgcgcatcat
      841 cgccgccagg ccctcctgca ggagctgctc ggtctcggta tccaccgagc tggtcgcctc
      901 gtccagcacg acgatggccg ggtcgtgcgc gacgacgcgc gcgaacgaca ggagctgccg
      961 ctgaccggag gacaacgtcg cggccccctc gccgaccgtg gcgccgtagc cgccttccaa
     1021 gcgctcgatg aagccggcgg cccgcacggc ctcggccgct ctccggatat cgtccgctcc
     1081 gaggccgagc cccaggtcga tattgcccgc gacggtgccc gagaacagga acacgtcctg
     1141 catcaccggc cgtatcgacg agcgtagcgc gtcgaggggc agctcccgta tcggcacgcc
     1201 gtccagccgt atctcgccgc gctgaggatc ccagagcctg gccaggagcg agccgacggt
     1261 gctcttgccg gccccggtat agccgacgat cgcgagggtc tcgcccggct ccaccgtgaa
     1321 cgacaggtcg cgaagcaccc agtcctcgcc cttgtacgcg aacca
//
LOCUS       DAOOLN010000677         1365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_128174_length_1365_cov_2.725954, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000677 DAOOLN010000000
VERSION     DAOOLN010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128174_length_1365_cov_2.725954"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1365)
                     /locus_tag="PLW80_08665"
     CDS             complement(<1..>1365)
                     /locus_tag="PLW80_08665"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB66622.1"
                     /translation="YVVELLRPDLDGVLACLGFEYLGIQTMMLSLVFLAREFKGKEGI
                     RPLALLALATIPVVTVVLGFTVSSHELLYVDPYVESIHGLTVVRFGMGPWYWVNVLFV
                     YGVYAYAGVEFARILIRSASVRKAQARLMLAGCAVPFAESILYLAGLTPGGIELSPVA
                     FALSGAFLASGIFRYGLFDVRPIARDAVFEHMHDAAIVVDADGFIADANRTARGLFPR
                     LDEDGSCLPLSELGSAYPELARLPAPGTLADTASLRLRVGEEERAFDVQASSISGRSG
                     RNRGSVILLYDVTERERLQACLIEQASTDDLTRVANRRRFYEVAEAELERAGRHGRPI
                     GFAILDMNGFKDINDGYGHQAGDDALRLVARLCVGELRSCDLVGRVGGDEFAFVLPES
                     DEEGVAAAAGKLRRLVGAATLTVGTATVRLSAAVGSVGCSGPVYPDLAELLTIADRRM
                     YADKP"
BASE COUNT          235 a          460 c          468 g          202 t
ORIGIN      
        1 cggcttgtcg gcgtacatgc gtcggtcggc tatcgtaagg agctcggcca ggtcgggata
       61 gaccgggccg gagcagccta ccgagccgac cgccgcggac agccgcacgg tcgccgtccc
      121 gacggtgagg gtcgccgcgc cgacgagtct ccggagcttg ccggcggcgg cggccacgcc
      181 ctcctcgtcg ctctccggta gcacgaacgc gaattcgtcg ccgcccacgc ggccgaccag
      241 gtcgcaggac cgcagctcgc cgacgcatag cctggccacg agacgcaggg cgtcgtcgcc
      301 ggcctggtgg ccgtagccgt cgttgatgtc cttgaagccg ttcatgtcca ggatggcgaa
      361 gccgatgggg cggccatggc gcccggcccg ctcgagctcg gcctcggcga cctcgtagaa
      421 gcgccgccgg ttcgccaccc tcgtcaggtc gtccgtcgag gcctgctcga tcaggcaagc
      481 ctggagccgt tcgcgctcgg tcacgtcgta cagtaggatg accgagccgc ggttccgtcc
      541 cgaccggccg ctgatgctcg acgcctgcac gtcgaaggcc cgctcttctt caccgacccg
      601 aaggcgtaat gaggcggtgt cggccaaggt tcccggagcg ggtagccggg ccagctccgg
      661 gtacgcgctc ccgagttccg atagtggaag gcacgacccg tcctcgtcga gccgagggaa
      721 gagcccgcgg gccgtcctgt tcgcgtcggc tataaagccg tcggcgtcca cgactatggc
      781 cgcgtcgtgc atgtgctcga atacagcgtc ccgggctatc ggcctcacgt cgaacagccc
      841 gtacctgaag ataccggagg cgaggaacgc gccgctgagc gcgaaggcga ccggcgatag
      901 ctcgatgccg ccgggcgtga ggccggccag gtagaggatc gactcggcga acggcacggc
      961 gcatcctgcc agcatgagcc tggcttgagc cttgcgcacc gaggcggaac gtatcagtat
     1021 gcgagcaaac tccaccccgg cgtacgcgta gacgccgtag acgaagagca cgttcaccca
     1081 gtaccacggg cccatgccga accgtacgac ggtgagcccg tgtatcgact cgacgtacgg
     1141 atcgacgtat aggagctcgt gcgacgagac ggtgaagccc aggacgacgg tgacgacggg
     1201 gatggtagcc agcgccagga gcgcgagcgg ccggatccct tccttgccct tgaattccct
     1261 ggccaggaat acgagcgaca gcatcatggt ctgtatgccc aggtactcga agcctaggca
     1321 ggccaggact ccgtccaggt cgggccggag cagctcgacg acgta
//
LOCUS       DAOOLN010000678         1365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_128179_length_1365_cov_2.617557, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000678 DAOOLN010000000
VERSION     DAOOLN010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128179_length_1365_cov_2.617557"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLW80_08670"
     CDS             complement(<1..212)
                     /locus_tag="PLW80_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66623.1"
                     /translation="MKRTLVALLLVAALAAPAFGQDVGAPWIDSGLPFAAAYDGTTAS
                     PTFAVALPTSILAFISNGIYADEIDMV"
     gene            complement(371..>1365)
                     /locus_tag="PLW80_08675"
     CDS             complement(371..>1365)
                     /locus_tag="PLW80_08675"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HPB66624.1"
                     /translation="RYLGVSRPVRLGADQSWRAVLAVPQSAYTDKLRRTDAKAFIILL
                     AFLATSFAVGWLVVDYVTRPIRTIADAVDALEPGARLPEEIVGFFDRRNELGRLARSF
                     AALKLRLDDSFGELEENLAEKDVLLREIHHRVKNNLQVVSSILSIESGSVDDDRARLA
                     FDKCQNRIQAMALVHEEVYRTGSFVELDMAEYLSRICESLRWGSGTKLTHACETALVV
                     RADDEDRLPLDKAIPCGLVVNELVANSLERAAPDRPVETVTVDFRRVGSGWRLSVSDD
                     GVAGAVGSTAVGDSPSSSCLDLELAEGLVAQLGGTIGREPLPDGGTVVIVDFPA"
BASE COUNT          246 a          415 c          488 g          216 t
ORIGIN      
        1 accatgtcta tctcgtccgc gtagatgccg ttggagataa aggccaggat cgaggtgggc
       61 agggccaccg cgaaggtagg cgaggctgtc gtgccgtcat acgcggccgc gaacggaagg
      121 cctgagtcta tccagggcgc tccgacatcc tgcccgaacg cgggcgccgc gagggcggcc
      181 acgagcagca gcgctacgag tgtgcgtttc ataaaagtcc tttccttata aaagtgcgca
      241 atgcatgagc ggagcctatg gctcccgcgc cgacggcgcc acgaagagta tagctcactg
      301 taaggaggaa gtaaaatttt tactaaaaaa cgcgacaaat gccaggattc tgtaaccttt
      361 cccgacggcg ctaggccggg aagtcgacga tgacgacggt gccgccgtcc gggagcggct
      421 cgcggccgat cgtgccgccg agctgggcca cgagtccctc ggccagctcg aggtcgaggc
      481 aggaacttga cggcgagtcg ccgacggcgg tgctgccgac ggcgccggct acaccgtcgt
      541 cggagacgga tagtcgccag ccggagccga ctcggcggaa gtccacggtg accgtctcga
      601 cggggcgatc cggggcggcg cgctccaggg agttcgcgac cagctcgttg acgacgaggc
      661 cgcagggaat ggccttatcc aggggcaggc ggtcttcgtc gtcggcccgg acgaccaggg
      721 cggtctcgca cgcgtgcgtc agcttcgtgc cggagcccca gcgcagggac tcgcagatcc
      781 tggagaggta ctcggccata tccagctcga cgaaggagcc ggtccggtag acttcctcgt
      841 gcacgagcgc catagcctgg atacggttct ggcacttgtc gaaggcgagc ctggcccgat
      901 cgtcgtcgac cgagccggac tcgatggaca gtatgctcga gacgacctgg aggttgttct
      961 tgacgcgatg gtggatctcg cgcagcagca cgtccttctc ggcgaggttc tcctcgagct
     1021 cgccgaagct gtcgtcgagc cggagcttga gcgcggcgaa cgaccgggct agcctgccga
     1081 gctcgttcct gcggtcgaag aagccgacga tctcctcggg cagtcgagcg cccggctcga
     1141 gggcgtcgac ggcgtcggcg atcgtccgga tcgggcgcgt gacgtagtct acgacgagcc
     1201 agccgacggc gaaggacgtg gccaggaagg cgagcagtat gatgaaggcc ttggcgtcgg
     1261 tccggcgcag cttatcggtg taggcgctct ggggcacggc caacacggcg cgccagctct
     1321 ggtcggcgcc gagcctgacg ggcctggaga cgccgaggta gcggg
//
LOCUS       DAOOLN010000679         1363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_128420_length_1363_cov_3.060398, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000679 DAOOLN010000000
VERSION     DAOOLN010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128420_length_1363_cov_3.060398"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..761)
                     /locus_tag="PLW80_08680"
     CDS             complement(96..761)
                     /locus_tag="PLW80_08680"
                     /inference="COORDINATES: protein motif:HMM:NF015181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HPB66625.1"
                     /translation="MAMRLASFIGWSNTGKTGFVEACAAALSARGVGVAAVKCVRHGG
                     SFNLEGKDTSRFFAAGAETALVAAEETVRVSRTPPDWGRAWVESLFPSADVILIEGRV
                     VEGATRVLVAGGATGLEGLKAPLDGFDALVTAERGLSALAAASGLSVFSPGESGAFIE
                     RYLSGGSMESRDVTVTTGGVEVPINPFIKETIENVVVGLVKPLKKTDIDGEIVIRIGP
                     ART"
     gene            complement(771..>1363)
                     /locus_tag="PLW80_08685"
     CDS             complement(771..>1363)
                     /locus_tag="PLW80_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66626.1"
                     /translation="VMLLEPATLAASRDALALRDALVSDGWRVEAPCTLCGLCPALAA
                     GPNHTCHDEAAWEVPTGVRRLAEAAGLDRELIKMTWYVVSASSRRVSASLDHVSASSN
                     HGPTASGDFGEGYYRVVSAPMLNKGGRVRYLLCGPSGRFPFSARQDDAAARSAGFFEL
                     GRYDLITVDKPERRENGWGFGPGTRLRRLPPGAERG"
BASE COUNT          234 a          462 c          475 g          192 t
ORIGIN      
        1 accgcaacgt gaacacgacg gccgactacg agtcgctact ggccgagctg gcagaggcgc
       61 cagccatgga acgggccgag cgaccgtagc gcccgctacg tccgcgccgg gccgatacgg
      121 atgacaatct cgccgtcgat atcggtcttc ttgagcggtt tgacgaggcc gacgacgacg
      181 ttctctatcg tctccttgat gaaggggttg atcggcacct cgacgccgcc ggtcgtcacg
      241 gtcacatccc tgctttccat gcttcctccc gataggtagc gttctatgaa ggccccggac
      301 tcgccggggg agaagactga caggccggag gcggccgcga gagccgaaag gccgcgctcg
      361 gcggtaacga gggcgtcgaa gccgtcgagg ggcgctttga gcccctcgag ccccgtcgcg
      421 ccgccggcga ccaggacccg cgtcgcgcct tcgacgactc ggccctcgat caggatcacg
      481 tcggcggagg ggaacagcga ctcgacccag gcgcggcccc aatcgggagg cgtcctcgag
      541 acgcgtacag tctcctccgc ggctacgagg gcggtctcgg ccccggcggc gaaaaagcgc
      601 gaggtatcct tgccttcgag gttgaagctg ccgccgtgcc gtacgcactt gacggcggcg
      661 actccgacgc cgcgcgcgga gagggcggcg gcgcaggcct cgacgaagcc ggtcttgccg
      721 gtgttcgacc agcctatgaa cgacgctaag cgcatagcca tggccgtatc ctagcctcgc
      781 tccgcgccgg gaggcaagcg ccgtaggcgg gttccgggac cgaagcccca gccgttctcg
      841 cgccgctcag gcttgtctac ggttatgaga tcgtagcgcc cgagctcgaa gaagccggcg
      901 ctccgggcgg ccgcgtcgtc ctggcgcgcg gagaacggga agcgtccgga tgggccgcag
      961 agcaagtagc gcacgcggcc gcccttgttg agcatcggcg ccgacacgac gcgataatac
     1021 ccctctccga agtctccaga cgccgtcgga ccgtggttcg acgaagctga aacatggtcc
     1081 aacgaagccg agacacgtct cgacgaagcc gagacgacgt accaggtcat cttgatgagc
     1141 tctcggtcga ggccggccgc ctcggccagg cgcctgacgc cggtaggcac ctcccaggca
     1201 gcctcgtcgt ggcaggtatg gttcgggccg gcggcgaggg ccgggcagag cccgcatagc
     1261 gtacagggcg cctcgacgcg ccagccgtcc gaaacgaggg cgtcgcgcag agccagggcg
     1321 tcgcgactcg ccgcgagcgt ggccggctcg agcagcatga cgg
//
LOCUS       DAOOLN010000680         1363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_128426_length_1363_cov_2.955657, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000680 DAOOLN010000000
VERSION     DAOOLN010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128426_length_1363_cov_2.955657"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="PLW80_08690"
     CDS             <1..535
                     /locus_tag="PLW80_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HPB66627.1"
                     /translation="TGELDRDLEDKLAMAGSDNLNHFLRWKYLSAIDQKWLDHLENME
                     ALREAVYLRHYAQKNPLLEYKLEGFELFDQMIEDIRKSVASTLYLVRIQRQEDRAVRP
                     VVSGGQSRHDEMGQFAGAVDGQAAAAGAKAQAAAGPMAQAAAPRNATVVRAYPKVGRN
                     DPCPCGSGKKYKHCHGA"
     gene            753..>1363
                     /locus_tag="PLW80_08695"
     CDS             753..>1363
                     /locus_tag="PLW80_08695"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HPB66628.1"
                     /translation="MSLNWREIEVVLSELDLVGAQIQKVVQPSYDTLLLSCYKPGAAT
                     ELLFCVAHGACRIHATSVRVPKAEKPQRFMELLRAQLRGLRIESVEQLGSERIVRLVA
                     GKDDERRVLYARLWSGAANIILCRPDGTIVDALARKPARGELAGGRYEPEAGPAPTKE
                     FSVRELPGEGSFNERVDAYYAEHGSELSRAALLERAAKYFSQRR"
BASE COUNT          262 a          448 c          440 g          213 t
ORIGIN      
        1 gacaggcgag ctggaccgcg acctcgagga caagctggcg atggccggct cggacaacct
       61 gaaccacttc ctgcgctgga agtacctgtc ggctatagac cagaagtggc ttgaccacct
      121 cgagaacatg gaagcgctcc gcgaggcggt gtacctcagg cactacgcgc agaagaaccc
      181 cctgctcgag tacaagctgg aaggcttcga gctgttcgac cagatgatcg aggacatccg
      241 caagagcgtc gcgagcacgc tgtacctcgt gcgcatccag aggcaggagg accgggccgt
      301 gcggcccgtg gtctcgggcg gccagagccg ccacgacgag atgggccagt tcgccggcgc
      361 cgtcgacggc caggccgccg cggccggcgc caaggcccag gccgccgccg gcccgatggc
      421 ccaggccgcg gcgccccgga atgccaccgt cgtccgcgcc taccccaagg tgggccgcaa
      481 cgacccctgc ccctgcggct cgggcaagaa gtacaagcac tgccacggcg cctgacaggc
      541 actgaagaaa gtgatgatgg aatttgaacc acggagtcac ggagacacgg agtaggaacg
      601 acactacgaa taaaaacaga gagggtttga agaaaaagcc ggttttctct ggattcgctc
      661 ttgcgctttc ctccgtgcct ccgtggtctg ctttttcaaa tcatctgctt ctttcctgta
      721 tcgctgtcgc gatactatga ataggttcat atatgtcgct taactggcgt gagatagagg
      781 tcgtactgtc ggagctcgac ctcgtcgggg cccagatcca gaaagtggtc caaccgtctt
      841 acgacacgct gctcctatcg tgttacaagc ccggcgcggc gacggagctc ctgttctgcg
      901 tggcccatgg cgcctgccgc atccacgcca ccagcgtcag agtgccaaag gccgagaaac
      961 cccagcgctt catggaactg ttgcgcgccc agctgcgcgg gctcaggata gagtcggtcg
     1021 agcagctcgg ttccgagcgc atcgtcaggc tggtcgccgg caaggacgac gagcggcgcg
     1081 tgctgtacgc gcgactgtgg tccggcgccg cgaacatcat actctgcagg cccgacggca
     1141 cgatcgtcga cgcgctggcc aggaagccgg cgcgcggcga gctggccggc gggcgctacg
     1201 agcccgaggc cggccccgcg ccgaccaagg aattctccgt gcgcgagctg cccggcgaag
     1261 gctccttcaa cgagcgggtc gacgcgtact acgccgagca cggctcggag ctgtcccgcg
     1321 ccgcgctgct cgagcgcgcg gccaaatact tcagccagcg ccg
//
LOCUS       DAOOLN010000681         1362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_128607_length_1362_cov_2.610559, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000681 DAOOLN010000000
VERSION     DAOOLN010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128607_length_1362_cov_2.610559"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="PLW80_08700"
     CDS             <1..804
                     /locus_tag="PLW80_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66629.1"
                     /translation="FIQGVEFELRIPKAFQGAESSIAWSLYSGIAPTPTSGRFDYEAR
                     LISTQPLPQRVSMSLILPIIASHGIKGGPFASLIPAIAGLADFPLMFKLSPIGKGLLP
                     AMESAEFKLTIRPVLRDEGGIRVSVTTIDGAEAQGVSVFIDDRMAENPGRLILARKGP
                     RVVRVRAEGYREEIVTVPVEAGVVSPLSVTLTPNAPFIAFQAPAGTYVTIDGKVVEPG
                     DMSGLSVEPGEHTLFFRIGDYSMTRKLMALRGKVYNVVLSVELDIVVTP"
     gene            835..>1362
                     /locus_tag="PLW80_08705"
     CDS             835..>1362
                     /locus_tag="PLW80_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPB66630.1"
                     /translation="MITVASWNVNGIRAVATKGFFEWLASSSADVVCLQETKADPSQL
                     GKEFFAPIAADGRVYRPYWASAKRRGYSGVAVYSLKEPRSVRPLGIDEFDDEGRYLEA
                     DFGELVVVSAYFPNSQEAGARLDYKLRFCDAVLARCDAIVAEGRHVSVAGDYNIAHEP
                     IDLTYPKANEGNPGYL"
BASE COUNT          249 a          434 c          427 g          252 t
ORIGIN      
        1 ttcatccagg gcgttgaatt cgagctgcgc atacccaagg ccttccaggg agccgagtcg
       61 tcgatcgcct ggtcgctgta ctccggcata gctccgactc cgacgtccgg ccgtttcgac
      121 tacgaagccc ggctgatatc cacccagccc ttgccgcagc gggtttcgat gagcttgatc
      181 ctgccgatca tagcctctca cggcatcaag ggcggcccgt tcgccagcct gataccggcc
      241 atagccggtc tggccgattt cccgctcatg ttcaaactct ctcccatcgg caaggggctc
      301 ttgccggcca tggaatccgc tgagttcaag ctgaccatca ggccggtcct ccgcgacgag
      361 ggcggcatca gggtcagcgt aacgaccatc gatggcgccg aggctcaagg cgtctcggtg
      421 ttcatagacg acaggatggc cgagaatcct ggtcggctca tcctggccag gaaggggccg
      481 cgcgtcgtac gggttcgcgc ggagggttat cgcgaggaga tcgtcacggt gccggtcgag
      541 gcgggcgtcg tctccccgct atcggtcacg ctgacgccga acgccccctt catagcgttc
      601 caggccccgg cgggcaccta cgtgacgata gacggcaaag tcgtcgagcc cggcgatatg
      661 agcggcctga gcgtggagcc gggcgaacat accctgttct tcaggatagg cgactactcg
      721 atgacgagaa aacttatggc cctgcgaggt aaggtctaca acgtcgtcct gtcggtggag
      781 ctcgacatcg tcgtgacgcc ataggataga aaccatcagc gaatcggagc ggctatgatc
      841 acagtggcat cgtggaacgt gaacggcata cgcgccgtcg ctaccaaggg gttcttcgag
      901 tggctggcct cgtcgtccgc cgacgtcgtc tgtctccagg agaccaaggc cgacccgtcg
      961 caactcggca aggagttctt cgccccgatc gcggccgatg ggcgcgtgta ccggccctac
     1021 tgggccagcg ccaagcgccg aggctattcg ggggtcgccg tgtactcgct taaggaaccg
     1081 cgatccgtgc ggcccttagg gatagacgaa ttcgacgacg aggggcgcta cctcgaggcg
     1141 gatttcggcg agctcgtcgt cgtcagcgcc tatttcccga attcccagga ggccggggcc
     1201 aggctcgact acaagctacg cttctgcgac gccgtgttgg cgcgttgcga cgctatcgtc
     1261 gccgaagggc gccacgtgtc ggtggcaggc gattataaca tcgctcacga gcccatcgac
     1321 ctgacctatc ccaaggccaa cgagggcaat cccggctacc ta
//
LOCUS       DAOOLN010000682         1361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_128718_length_1361_cov_3.094946, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000682 DAOOLN010000000
VERSION     DAOOLN010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128718_length_1361_cov_3.094946"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..734
                     /locus_tag="PLW80_08710"
     CDS             249..734
                     /locus_tag="PLW80_08710"
                     /inference="COORDINATES: protein motif:HMM:NF018958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HPB66631.1"
                     /translation="MMTNDILRRLRYSLRLSDDAMLEMLSLAGSPIGKDRLLSYFKRD
                     DEPGYAECPKAALSSLLDGMILKFRGPGPKGAAPATVEPLDNNMVLKKLRIALALKED
                     DMLSVMRLGEAELSAGELRALFRGRGQRNYVECLDQYLRGFLTGLARRSDGDDTTRKS
                     V"
     gene            751..1086
                     /locus_tag="PLW80_08715"
     CDS             751..1086
                     /locus_tag="PLW80_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66632.1"
                     /translation="MVGRPSRRSALPGRLRFIVKRAIRTLGAFAASMLCLSAVVNASF
                     EATVRSQIDEAAMGAVMASGSSSPEAILALRAEIRAGLERSYGLDAPSWLRVVRQTGR
                     ILSDWNIEM"
BASE COUNT          272 a          377 c          437 g          275 t
ORIGIN      
        1 cggctatggc gcgagcctcc gcgtcgagcg ccggaaggcc ctcgagcgtc ctgagcacgg
       61 cgaacaggtt cacccgcgcc gatgtcgttg gatcgaccat acgttttgcc cccctcgagt
      121 tcgatgaaac gtatcgagcg aatgctcgct ctattagtta gaccacgatt tttcgatttg
      181 cgcaacaaga atcttcgcgt tgagcgcggt tgctcgagga gcctgaaccg gtataccatc
      241 gccttaccat gatgaccaac gacatactca ggcgcctacg atattccctc cggttgagcg
      301 acgacgcgat gctcgagatg ctctccctgg ccggctcccc gataggcaag gaccggctac
      361 tatcgtattt caagcgcgac gacgagccgg gctacgccga gtgcccgaaa gccgcgttgt
      421 cgtcgctact cgacgggatg atcctcaaat tcagggggcc ggggccgaag ggcgccgcgc
      481 cggctaccgt cgagccgctc gacaataaca tggtactcaa gaagctcagg atagccctgg
      541 cgctcaagga agacgatatg ctgtcggtca tgaggctcgg cgaggcggag ctgagcgccg
      601 gcgagctgcg cgcattgttc cggggcaggg ggcagcggaa ctacgtcgag tgcctcgacc
      661 agtacctgcg cggcttcctg accgggctcg cgaggcgctc ggacggggac gatacgacgc
      721 ggaagtccgt ctagcccata cggaagctgg atggtaggaa gaccgtctcg ccgctcggcc
      781 ctgccgggcc ggctcaggtt catagtaaag agggccatac ggacgctcgg ggccttcgcg
      841 gcctcgatgc tctgcctctc ggccgtggtc aacgcctcgt tcgaagccac ggtgcgctct
      901 cagattgacg aagccgccat gggagcggtg atggccagcg gttcgtcgtc gcccgaggcg
      961 atactggcgc tcagggccga gatccgcgcc ggcctcgaac gttcgtacgg cctcgatgcc
     1021 ccctcgtggc tacgggtggt caggcagacg ggccgtattc tgagcgattg gaacatcgag
     1081 atgtagaaca accatgcttc cgcatcctat tggttctctg tggaggatgg acttttgatg
     1141 aaatatgaag gggtggatag tgaaaaaaga aaagaggctt tgcatcgcat cgcccaagag
     1201 ctattctgat gtgtctcaat tctcgcgggt taggtgccat ggtctttttt ttgaggtcat
     1261 gagacgagat gaaggcattg ttttggattg ttgaaaaggg attcggtgga ggcaggcacg
     1321 attatgattt gggtaatgtc gagcgagcag cagtaaagca g
//
LOCUS       DAOOLN010000683         1361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_128779_length_1361_cov_2.137060, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000683 DAOOLN010000000
VERSION     DAOOLN010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128779_length_1361_cov_2.137060"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLW80_08720"
     CDS             complement(<1..234)
                     /locus_tag="PLW80_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase"
                     /protein_id="HPB66633.1"
                     /translation="MDATGKLSILADAAKYDASCSSSGSARDNTPGGLGNGAVGGICH
                     SWAADGRCVSLLKVLLSNACVYDCAYCVNRRSND"
     gene            complement(314..>1361)
                     /locus_tag="PLW80_08725"
     CDS             complement(314..>1361)
                     /locus_tag="PLW80_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66634.1"
                     /translation="ILLDLHPDYTLLRESAVLGALSADDEMRRFQTALFYRTNERSIR
                     IMSDHPAIRERVFGPLRRLPDAAMFEHFLGLGSGKPTFTKIRSDAAPLPIGLDGSCDK
                     VEVTSVIGSRAFGLYCRRRFPLGLFVSPPEWYFSMKYFVNRVYDYVCAMRVHGHSLAA
                     IGFDAPRAVDGVDPDYRFLRNAHKLMLHLQELIRTRVGAYGVEVDYSWMSATRFKRLV
                     DIDGDGFRRDFDAMVLDGGLLMPSEAKRYRVLRGRVLAKSGDRVMEGRAQDLASLPEG
                     LRYETIYQDADRFKIRIPYSWSDLAFFAFSAIGSRMARIVEGRLMKALPALGMPPADA
                     RRYQLVMHEQARRR"
BASE COUNT          254 a          419 c          443 g          245 t
ORIGIN      
        1 gtcgttggat cggcgattga cgcagtaggc gcagtcgtag acgcaggcgt tcgagaggag
       61 caccttgagc agggagacgc agcggccgtc cgcggcccag ctatggcaaa tacccccgac
      121 cgccccgtta cccagccctc cgggtgtgtt gtccctggcg ctaccggacg atgaacacga
      181 ggcgtcgtat ttggctgcgt ccgctagtat cgatagcttc cccgttgcat ccatgcccgg
      241 acctttcccg agcatgatac tatacgaatg tatagtagtc aacgagatcg gctatggaac
      301 ggccagctca gcatcagcgc ctgcgcgcct gttcatgcat gacgagctgg tagcgtctgg
      361 cgtcggcggg aggcatgccg agcgccggaa gggctttcat gagtcgtcct tcgacgatgc
      421 gggccatcct ggagcctatc gcgctgaacg cgaaaaacgc gaggtccgac caggagtacg
      481 gtatcctgat tttgaagcgg tccgcgtcct ggtagatagt ctcgtagcgt aggccttcgg
      541 gcaggctcgc aaggtcctgc gccctgcctt ccatgactct gtcgccagat ttggcaagga
      601 cgcgcccgcg taggacgcga tagcgtttgg cctcgctcgg catgagcagc cccccgtcca
      661 ggaccatagc atcgaagtcg cgtcggaacc cgtctccgtc tatgtcgacg agccgcttaa
      721 acctggtggc ggacatccac gagtagtcga cctcgacgcc gtaggcgcct acgcgcgtgc
      781 ggatgagctc ctgtaggtgg agcatcagct tatgggcgtt gcgtaggaag cggtagtccg
      841 ggtcaacgcc gtcaaccgcg cgcggcgcgt cgaagccgat cgcggcgagc gaatggccgt
      901 ggacccgcat ggcgcagacg tagtcgtaga cgcggttgac gaagtacttc atcgagaaat
      961 accactccgg cggcgatacg aacagcccga gcgggaagcg cctacggcag tagaggccga
     1021 aggcccggct accgatgacc gacgttacct ccaccttgtc gcagctaccg tccagcccga
     1081 tgggcagtgg agccgcgtcc gagcgtatct tggtgaaggt cggcttgccg gagccgagcc
     1141 ccaggaaatg ctcgaacatc gctgcgtcgg gcaggcgccg caaggggccg aacacccgct
     1201 ctcgtatggc cggatggtcg ctcatgatcc tgatgctgcg ctcattcgtc cggtagaaca
     1261 gggcggtctg gaagcgcctc atctcgtcgt ccgccgacag cgctccgagc accgccgact
     1321 ccctgagcag ggtatagtca gggtgcaggt cgagcaggat g
//
LOCUS       DAOOLN010000684         1357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_129319_length_1357_cov_3.183564, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000684 DAOOLN010000000
VERSION     DAOOLN010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129319_length_1357_cov_3.183564"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PLW80_08730"
     CDS             complement(<1..733)
                     /locus_tag="PLW80_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPB66635.1"
                     /translation="MFQKQVMMRRVLYALAPITALAIWLYGPRVLATLAVSLGVGILV
                     EYLFEKRKGGKVSEAVLVTGTLFALSMPPAAPLWIVAIGIAFAVFMAKEVYGGFGRNV
                     FNPAIAGRLFVYISFAGALGGSFFEPGGFGAAAGSLFGRPDALSAATPLAVMRSGGSV
                     PTLDLLLGTRAGSIGESSVVLIAVAAVYLVATKTAQWRLIVSTLLGGAIVTGGLVVAG
                     VARALPMDSLLAGSFLFVAVFMATDP"
     gene            874..>1357
                     /locus_tag="PLW80_08735"
     CDS             874..>1357
                     /locus_tag="PLW80_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB66636.1"
                     /translation="METTRDRILEVAVGAFAARGYEAVGVQELCESSGITKPTLYYHF
                     GSKEGLLAALFAERFGGFNGRVATAAAYPGNRRGDLAGVLLATIGAFIEAARDDSDFS
                     RVRLSSAFCPPSSERYRVARPYLEALYGTIAATFESAALEHGNMAGRAAQYAASFIGA
                     S"
BASE COUNT          244 a          455 c          449 g          209 t
ORIGIN      
        1 ccgggtcggt ggccatgaac accgcgacga acaggaagct accggccagg aggctatcca
       61 tcggcaacgc ccgcgcgacg cccgcgacta ccaggccccc ggtgacgatc gcgccgccga
      121 gtagcgtcga gacgatcagc cgccactggg ccgtcttcgt ggcgaccagg tagaccgcgg
      181 cgacggcgat caggacgacg ctggactcgc ctatggaacc cgcgcgcgtg ccgagcagca
      241 agtcgagcgt cggcacggaa ccgcccgaac gcatgacggc gagcggggtg gccgcggaga
      301 gggcatccgg gcggccgaac aggctcccgg ccgccgcgcc gaagccgccg ggctcgaaga
      361 acgagccgcc gagcgctccg gcgaaggaga tatacacgaa aagccgtccg gctatggccg
      421 ggttgaacac gttgcggccg aatccgccgt agacctcctt ggccatgaac acggcgaagg
      481 ctatacctat cgcgactatc catagcgggg ccgccggcgg catcgacagc gcgaacagcg
      541 tcccggtcac cagtaccgcc tcgctgactt tgccgccctt gcgcttttcg aacaggtatt
      601 ctaccagaat accgacgccg aggctcacgg ccagggtcgc caggaccctt ggcccgtaca
      661 gccatatcgc caaggccgtt atcggcgcga gcgcgtagag caccctgcgc atcatcactt
      721 gcttctggaa cacgtatcgt ctcctcgtta acgcgcgatc cgttccgggg atcgccttcg
      781 cccccggaac tagtagtata gacgcggcgg cccaatccgc caacgggttt cgtggtcttg
      841 cggcaatata ccgaacggta tatagtcgta tccatggaaa cgacgcgaga caggatactg
      901 gaagtcgccg tgggggcgtt cgccgcccgg ggttacgagg cggtcggcgt ccaggaacta
      961 tgcgagtcat cgggcataac caagccgacg ctgtactacc acttcggtag caaggaaggc
     1021 ctgctcgcgg ccctcttcgc ggagcgtttc ggcggcttca acggtcgggt ggcgacggcc
     1081 gccgcgtacc cgggcaacag gcgcggcgat ctcgccggag tcctgttggc gacgataggg
     1141 gccttcatcg aggcggcgcg cgacgactcg gatttctcgc gggtgcggct gtcgtcggcc
     1201 ttctgtccgc cgtcgagcga gcgttaccgc gtcgccaggc cgtacctgga ggcgctgtac
     1261 ggaacgatag ccgctacctt cgagtcggcc gcgctggagc acggtaacat ggccggtcgc
     1321 gcggctcaat acgccgcgtc cttcatcggc gcctccg
//
LOCUS       DAOOLN010000685         1356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_129454_length_1356_cov_3.005380, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000685 DAOOLN010000000
VERSION     DAOOLN010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129454_length_1356_cov_3.005380"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1356)
                     /gene="metG"
                     /locus_tag="PLW80_08740"
     CDS             complement(<1..>1356)
                     /gene="metG"
                     /locus_tag="PLW80_08740"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPB66637.1"
                     /translation="DVFARACRLRSYETLYVCGTDEYGTATETKAAEEGVSPAELCSR
                     FHEIHKAIYEWFGIGFDKFGRTSTPRQKEIVQSLFKDLDEAGFITEQTIEQLYCEPCG
                     RFLADRYVRGTCPHCGYDDARGDQCEACGKLLEPSELQRPKCSTCGASPKPRSTRHLY
                     IDLPAIKPRLEDWMKDASVKGFWTNNAIQMTQAWIRDGLKPRAITRDLKWGIPVPKPG
                     FEDKVFYVWFDAPIGYLSISACAGDELGFDWKDWWMKPDEVELFQFIGKDNIPFHTVI
                     FPSTLLGSGRDWTMLHHMSSTEYLNYESSKFSKSKGVGVFGTDAMETGIPSDVWRFYV
                     FYNRPEKSDVFFTWADFLEKVNGELIGNLGNLANRTLSFVQRFYDGAIPAGKPDEAFW
                     AEVRALEADVADKLERAELRDAFRAAFQISDIANKRFQAEEPWKARTADPDKAASLLS
                     D"
BASE COUNT          222 a          420 c          456 g          258 t
ORIGIN      
        1 ggtcggacag gaggctcgcc gccttgtcgg ggtcggccgt cctggccttc catggctcct
       61 cagcctggaa gcgcttattg gctatatccg atatctggaa ggcggcgcgg aacgcgtcgc
      121 gcagctcggc gcgctcgagc ttatccgcca cgtccgcctc gagcgcgcgg acctccgccc
      181 agaacgcctc gtccggcttg ccggccggta tggcgccgtc gtagaagcgc tgcacgaacg
      241 atagcgtccg gttggccagg ttgcccaggt tgccgatcag ctcgccgttg accttctcga
      301 ggaagtcggc ccaggtgaag aatacgtcgc tcttctcggg gcggttatag aagacataga
      361 agcgccagac atcggacggt atgccggtct ccatcgcgtc ggtgccgaag acgccgacgc
      421 ccttggactt ggagaacttg ctcgactcgt agttcaggta ctcggtactg gacatatggt
      481 gaagcatcgt ccaatcccgg ccggaaccga gcagggtgct cgggaagatc accgtgtgga
      541 acgggatatt gtccttgcct atgaactgga acagctccac ctcgtcgggc ttcatccacc
      601 agtccttcca gtcgaagccg agctcgtcgc cggcgcaggc ggagatggac aggtagccga
      661 tgggcgcgtc gaaccagacg tagaagacct tgtcctcgaa gccgggcttg ggcaccggga
      721 taccccactt gaggtcgcgc gtgatggcgc gcggtttgag cccgtcgcgt atccaggcct
      781 gggtcatctg gatggcgtta ttggtccaga agcccttgac gctcgcgtcc ttcatccagt
      841 cctcgagcct gggcttgatg gccggcaggt cgatgtacag gtgcctggtc gagcgcggct
      901 tcggcgaggc cccgcaggtc gagcacttgg gccgctggag ttccgagggc tcgagtagct
      961 tgccgcaggc ctcgcactgg tcgccacgcg cgtcgtcgta gccgcagtgc gggcaggtgc
     1021 cgcgtacgta gcggtcggcc aggaagcggc cgcacggctc gcagtacagc tgctcgatgg
     1081 tctgctcggt gatgaaaccg gcctcgtcga gatccttgaa cagcgactgc acgatctcct
     1141 tctggcgagg cgtcgaggtc cggccgaatt tatcgaagcc tatgccgaac cattcgtaga
     1201 tggccttatg gatctcgtgg aagcgcgagc acagctcggc ggggctcacg ccctcctcgg
     1261 ccgccttggt ctcggtggcc gtgccgtact cgtcggtacc gcagacgtag agggtctcgt
     1321 agctgcggag ccgacaggcg cgggcgaaca cgtcgg
//
LOCUS       DAOOLN010000686         1356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_129499_length_1356_cov_2.261337, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000686 DAOOLN010000000
VERSION     DAOOLN010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129499_length_1356_cov_2.261337"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="PLW80_08745"
     CDS             <1..979
                     /locus_tag="PLW80_08745"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HPB66638.1"
                     /translation="GKHMHKPEYETCCAFGALVLNDDLDTIMLCNDICNRSGLDTISA
                     GTVVAFALECYERGVITKEDADGLELRWGDSGAIVELLRMMAERRGLGDLLADGVAIA
                     AKRLGKGAEELAVHAGGQEPGMHDGRMDPMMAVSFAADPTPGRHTISAGVYYNVSHLW
                     EYVSWAPKVTKPYPKSKEYAASDEETLKSVAGACMKQLLDATGGCLFALTTGLQHWRF
                     FDWLAAATGDARTPDEWMEAGRRIQTARAAFGARERLADEGKAWSRNYSLANPRIYGD
                     PPLREGPLAGKTVPLAEMVVRYRREFGWDEDSGAPRPETMRTLGLSVHS"
     gene            complement(996..>1356)
                     /locus_tag="PLW80_08750"
     CDS             complement(996..>1356)
                     /locus_tag="PLW80_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66639.1"
                     /translation="AEEVKAVESAGTDWIHVDVMDGHFVPNITIGPDVVRAIRKATNL
                     PLDVHLMIEKPERYLHAFVEAGADLSSAREGCDLIIRTDLVEAVLGAVGRLGLFEGDA
                     RGQAEISAVGHAVTYSA"
BASE COUNT          225 a          465 c          458 g          208 t
ORIGIN      
        1 cggaaaacac atgcacaagc ccgagtacga gacctgctgc gcgttcggcg ccctcgtcct
       61 gaacgacgac ctcgatacca tcatgctatg caacgacatc tgcaaccgct ccggcctgga
      121 cacgatctcg gccggtaccg tcgtggcctt cgccctcgag tgctacgagc gcggcgtgat
      181 caccaaggaa gacgccgacg ggctcgagct tcgctggggc gactcgggcg ccatcgtcga
      241 gctgctacgg atgatggccg agcgccgggg tctcggcgac ctcctggccg atggcgtcgc
      301 gatagccgcg aagcgcctgg gcaagggcgc cgaggaactg gccgtccacg cgggcggcca
      361 ggagccgggc atgcacgacg gccgcatgga cccgatgatg gcggtatcgt tcgccgccga
      421 cccgacgccg gggcggcaca ccatctcggc cggcgtgtac tacaacgtgt cgcacctctg
      481 ggagtacgtc agctgggcgc cgaaggtgac gaagccctat ccgaagtcca aggaatacgc
      541 ggcgagcgac gaggagacgc tcaagtcggt cgccggcgcc tgcatgaagc agctcctcga
      601 cgcgaccggc ggttgcctct tcgcgctgac gaccggcctc cagcactggc gcttcttcga
      661 ctggctggcc gcggcgaccg gcgacgcccg tacgccggac gagtggatgg aggccggccg
      721 ccgcatccag acggcccgag cggcgttcgg cgcccgcgag cggctcgccg acgagggcaa
      781 ggcctggtcc aggaactact cgctcgccaa tcctcgtatc tatggcgacc cgccgctccg
      841 cgagggcccg ctcgcgggca agacggtgcc gctcgccgag atggtcgtca ggtaccgcag
      901 agagttcggc tgggacgagg actccggcgc cccgaggccg gagacgatga ggacgctcgg
      961 cctatcggta cacagctagg gcgcgagatg gtcggctacg cgctataggt aacggcgtgg
     1021 ccgaccgcgc tgatctcggc ctgcccgcgg gcgtcgccct cgaacaggcc gagcctgccg
     1081 accgcgccga gcacggcctc gaccaggtcg gtcctgatga tcaggtcgca gccctcgcgc
     1141 gcgctcgaca ggtcggctcc cgcttcgacg aatgcgtgca ggtaccgttc gggtttctcg
     1201 atcatcaggt gcacgtccag gggcaggttc gtcgctttgc ggatggccct gaccacatcg
     1261 ggcccgatgg ttatgttcgg gacaaaatgt ccatccatga catccacgtg aatccagtcc
     1321 gttcctgctg attcgacagc cttgacctct tccgcg
//
LOCUS       DAOOLN010000687         1355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_129632_length_1355_cov_2.287692, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000687 DAOOLN010000000
VERSION     DAOOLN010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129632_length_1355_cov_2.287692"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PLW80_08755"
     CDS             <1..292
                     /locus_tag="PLW80_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66640.1"
                     /translation="LGMELGRARLEYAALRQEGWASDLAQKRAALRAGGGSESEYLTG
                     MIEALGSLAEAYGTLAEYRGALVSLDALAGSPSPAAPATAPMEAPTEAPQAP"
     gene            complement(329..>1355)
                     /gene="glpK"
                     /locus_tag="PLW80_08760"
     CDS             complement(329..>1355)
                     /gene="glpK"
                     /locus_tag="PLW80_08760"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HPB66641.1"
                     /translation="RAERGELAFGTVDSWLIYNLTGRHATDATNASRTMLFNIREGRW
                     DQEILRMLKIPEAILPEVLPSSADFGYTRKDLFGCEIPVCGCAGDQQAALFGHACFQV
                     GASKNTYGTGCFALMNTGSEPVESRHNLLTTIAWDLGKGYTYALEGSVFIGGAVIQWL
                     RDEMRLLSDSAESERLARLVKDTGGVVLVPAFVGMGAPYWDAEARGAALGVTRGTTKA
                     HFVRAALESVAYQSRDLLDAMERDAGASIPFIKADGGASANSFLMQFQADVLDKPVIL
                     PEVGEVTALGAAYLAGLRVGYWSGLDELERNWRRKRDFMPEMAAETRDRLLERWSKAV
                     EVTRLFK"
BASE COUNT          196 a          462 c          490 g          207 t
ORIGIN      
        1 gctgggcatg gagctgggcc gcgccaggct cgagtacgcg gcgctcaggc aggagggctg
       61 ggcctccgac ctggcgcaga agcgggcggc cctgcgggcc ggaggcggct cggagtcgga
      121 gtacctgacc ggcatgatcg aggcgctcgg ctcgctggcc gaggcctacg gcacgctggc
      181 cgagtaccgc ggcgccctcg tgtcgctcga cgcgctcgcg ggctcgccgt cgcctgcggc
      241 gccggcgacc gctccgatgg aagcgccgac tgaagcgccg caggcgccat aaccgagaaa
      301 cggtgtccga cggcctccgg gacgcggctt acttgaagag gcgcgtcacc tcgacggcct
      361 tggaccagcg ctcgagcaga cgatcgcgcg tctcggctgc catctcgggc atgaagtcgc
      421 gcttgcggcg ccagttgcgc tctagctcgt ccagcccgga ccagtagcct accctgaggc
      481 cggccaggta ggcggcgccg agcgcggtca cctcgccgac ctccggcagg atgaccggct
      541 tgtcgagcac gtcggcttgg aactgcatga ggaagctgtt ggcgctggcg ccgccgtccg
      601 ccttgatgaa cggtatcgag gcgccagcgt cgcgctccat cgcgtcgagc aggtcgcgcg
      661 actggtaggc taccgactcg agggctgccc gcacgaagtg ggccttggtc gtgccgcggg
      721 tgacgccgag cgcggcgccg cgggcctcgg cgtcccagta cggcgcgccc atgccgacga
      781 aggccggcac gaggacgacg ccgccggtgt ccttgaccag ccgcgccagc cgctcgctct
      841 cggccgagtc ggataggagg cgcatctcgt cgcgtagcca ttggatcacc gccccgccta
      901 tgaacacgga accctcgagc gcgtaggtat agcccttgcc caggtcccag gctatcgtcg
      961 tgagcaggtt gtgcctcgac tcgaccggct cggagccggt gttcatcagc gcgaagcaac
     1021 cggtgccgta ggtgttcttg ctggcgccga cctggaaaca ggcgtgcccg aatagcgcgg
     1081 cctgctggtc gccggcgcag ccgcagaccg gtatctcgca gccgaagaga tccttgcgcg
     1141 tgtagccgaa gtcggccgag ctcggcagca cctcgggcag gatggcctcg gggatcttga
     1201 gcatcctgag tatctcctgg tcccaacggc cctcgcggat gttgaacagc atggtgcggc
     1261 tggcgttcgt ggcgtcggtc gcgtgcctgc cggtcaggtt gtagatcagc caggagtcga
     1321 cggtgccgaa cgccagctcg ccgcgctcgg cccgg
//
LOCUS       DAOOLN010000688         1352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_130102_length_1352_cov_2.156515, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000688 DAOOLN010000000
VERSION     DAOOLN010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130102_length_1352_cov_2.156515"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1352
                     /gene="priA"
                     /locus_tag="PLW80_08765"
     CDS             <1..>1352
                     /gene="priA"
                     /locus_tag="PLW80_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPB66642.1"
                     /translation="LCRAFDLPVDRQWSYAEPEGLSAPVGARVEATLGRRAAVGWVVA
                     RSDTVDCDPAIVKPYLRLVDAEPLFGPETLRLAEWLSGMYYCSLGEALSAMTPSAERE
                     SRRKAKKPASGKGYVGDAGSVGDESSVGDAGSVGDESSVGDAASFDDVRVAPTPLVPS
                     AEQAAALGRVLSGRDGRWYLYGPTGTGKTEVFLEAAEATLAEGRGVIYLVPEIALTRQ
                     VVEAIKDRFGDTCAVIHSGLTGPKKLTEWRRILRGEARVVVGARSAVFAPVSDLGLIV
                     LDEEHEGSYKAGSSPRYHARQVAMRRAGTEGARLVMGSATPSVESWRLMGEGGMERLV
                     LSRRLAGGDMPAIELVDMRAEGGALSKRLIDEVRRVKREGGQSILFLNRRGFSYFWSC
                     RSCGAEMKCRHCSVGMTYHKERGRLVCHYCGYQAPPPSACPVCGSLDTGWAGFGTERI
                     "
BASE COUNT          199 a          467 c          491 g          195 t
ORIGIN      
        1 ctctgccgcg ccttcgacct gcccgtcgat aggcagtgga gctacgcgga gcccgagggc
       61 ctgtccgcgc ccgtaggcgc ccgggtcgag gcgacgctcg gccgcagggc cgcggtcggc
      121 tgggtcgtcg ccaggagcga taccgtcgac tgcgacccgg ccatcgtcaa gccgtacctg
      181 cgcctcgtcg acgccgagcc cctgttcggc ccggagacgc tcaggctcgc ggagtggcta
      241 tccggcatgt actactgctc gctcggcgag gcgctgtcgg cgatgacgcc gtcggccgag
      301 cgggagagcc ggcgcaaggc caagaagccg gcttcgggga agggctacgt cggagacgcc
      361 ggctccgtcg gagacgagag ctccgtagga gacgccggct ccgtaggaga cgagagctcc
      421 gtaggagacg ccgcttcgtt cgacgacgtg cgcgtcgccc cgaccccgct cgtgccgtcg
      481 gccgagcagg ccgccgcgct cgggcgcgtg ctgtccggcc gcgacggtcg ctggtacctg
      541 tacggcccca ccggcacggg caagaccgag gtcttcctcg aggccgccga ggcgaccctc
      601 gccgagggtc gcggcgtcat ctacctcgtg cccgagatag cgctgacgcg gcaggtggtc
      661 gaagcgatca aggaccgatt cggcgatacc tgcgcggtca tccactcggg cctgaccggt
      721 cccaagaagc tgaccgaatg gcggcgcatc ctgcgcggcg aggccagagt cgtcgtaggc
      781 gcccgtagcg ccgtattcgc tccggtcagc gacctcggac tgatcgtgct cgacgaggag
      841 cacgaaggct cgtacaaggc cggctcgtcg ccgcgctacc acgcccgcca ggtggccatg
      901 cgccgcgccg gcacggaagg cgccaggctg gtcatgggct cggccacgcc gagcgtcgag
      961 tcgtggcggc tgatgggaga gggcggcatg gagcggctcg tcctgtcacg ccggctggcc
     1021 ggcggcgaca tgccggcgat agagctcgtg gacatgaggg ccgagggggg cgccctctcg
     1081 aaacgactga tcgacgaggt cagacgggtc aagcgggaag gcggccagtc catcctgttc
     1141 ctcaaccgtc gcggcttctc atacttctgg agctgccgga gctgcggggc cgagatgaag
     1201 tgccgtcact gctcggtcgg catgacctac cataaggagc ggggccgcct cgtctgccac
     1261 tactgcggct accaggcgcc tccgccttcg gcctgtcccg tctgcggctc gctcgacacc
     1321 ggctgggcag gcttcggcac cgagcgcatc ga
//
LOCUS       DAOOLN010000689         1351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_130160_length_1351_cov_3.491512, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000689 DAOOLN010000000
VERSION     DAOOLN010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130160_length_1351_cov_3.491512"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..615)
                     /locus_tag="PLW80_08770"
     CDS             complement(46..615)
                     /locus_tag="PLW80_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HPB66643.1"
                     /translation="MTRTIEQAVDGLVVKALRTWDMIRSGDRVLIGASGGKDSAVMAR
                     DLALKKRHGRLDAELVGLHIRNDFSPNALSRRLEDEFAEWGIPLVTVDVAVEGRLKPG
                     RKMNCYWCSTQRRTELIRYAMAEGFSAVALGHHLDDALETLFMNMMRKGELSTMPPVV
                     SYRKYPLRVIRPLYLGFPEQLIQAYSVPS"
     gene            763..>1351
                     /locus_tag="PLW80_08775"
     CDS             763..>1351
                     /locus_tag="PLW80_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPB66644.1"
                     /translation="MGNETTASSGAFGETELTPRHSNGLAILALNILLMIGALVLFIV
                     CVIALDGGGATPLAIIGVIGSLLWLCVVGPILFGGIKVLKPNEAMVFTLFGKYYGVLR
                     EPGIFFVNPFASAVHPASTVELSTASTVDKAVATGRPAGTTVKTVSRTLSLKAMTLNN
                     DKQKINDSLGNPIIIGIVVVWKVVNPTKAVFNVDNY"
BASE COUNT          256 a          415 c          393 g          287 t
ORIGIN      
        1 ttgggacttg gttgaaatcc ttgttagaaa gactagattc tcggttcaac tcggaactga
       61 atacgcttga atgagttgtt caggaaaccc caagtacagt gggcgtatca cccggagcgg
      121 atacttgcga tagctcacca ccggcggcat cgtcgacagc tcgcccttgc gcatcatgtt
      181 catgaacagc gtctcgagcg cgtcgtccag gtgatggccg agcgccaccg cggagaagcc
      241 ctcggccata gcgtagcgta tcaactcggt gcgccgctgg gtggagcacc agtagcagtt
      301 catcttccgg cccggcttga gcctgccctc gaccgccacg tcgaccgtga ccagcggtat
      361 tccccattcg gcgaattcgt cctcgagtcg cctcgacagg gcgttcggcg agaagtcgtt
      421 gcgtatatgg aggccgacca gctcggcgtc caggcgtcca tggcgcttct taagcgccag
      481 gtcccgcgcc atgacggccg agtccttgcc gcccgaggcg ccgatcagta cgcgatcgcc
      541 cgaccgtatc atgtcccagg tcctaagcgc cttgacgacg aggccgtcga ccgcctgctc
      601 tatggttcgt gtcacggttc gttctactat cgatgcgcgg cccgccgcaa gcccagtccg
      661 catcgaattc gccataggcc tcttgaattc cggcgcgcgt aacgctatta tctagatgat
      721 atcatattga tatcttttag gtatcgttat aaggagacct ccatgggtaa cgaaacgact
      781 gcatcttcgg gagccttcgg cgagacggag ctgacgcctc gacattcgaa cgggctggcc
      841 attctggctc tgaatatcct gttgatgata ggggcgctcg tcctgttcat cgtatgcgtg
      901 atcgccctgg acggcggagg cgcgactccg ctcgctatta tcggagtgat aggttccctg
      961 ctctggctgt gcgtcgtcgg acccatcctg ttcggcggca tcaaggtgct caagccgaac
     1021 gaggcgatgg tcttcacgct gttcggcaag tactatggcg tgctccggga acccggcatc
     1081 ttcttcgtga acccattcgc gtcggcggtc catccggcct cgaccgtcga gctgtccacc
     1141 gcctcgaccg tcgacaaggc ggtcgcgacg gggcgtccgg ccgggacgac ggtcaagacc
     1201 gtctccagga ctctgtcact caaggcgatg acgctcaata acgacaagca gaagatcaac
     1261 gactcgctcg gcaacccgat catcatcggc atcgtggtcg tctggaaggt ggtcaacccg
     1321 accaaggcgg tgttcaatgt cgacaactac g
//
LOCUS       DAOOLN010000690         1350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_130338_length_1350_cov_2.533591, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000690 DAOOLN010000000
VERSION     DAOOLN010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130338_length_1350_cov_2.533591"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="PLW80_08780"
     CDS             complement(<1..849)
                     /locus_tag="PLW80_08780"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPB66645.1"
                     /translation="MATRIETLASILRAAPGSVFIQAHDVPDPDAIASAFGLRHLLSS
                     YGVESTIVYEREFEKIDAQRMVELLGIELSLASDVPTIGPDDWAVIIDGQRGNANMVD
                     LACRDVAAIDHHALRDDAAYRYADIRPEVGSCAAMIAEYFAEAGMAPPGLVATALMYG
                     IFVDTDNLTRGVSDLDARMFYELRAWADADLIRKLRGSQITLRDLAMYAEALGKVETH
                     GRLAFLGLRDADDSLVGAANDLVLSVDEVDVSLSYSLREDGIKLSTRSLVEAVRADEL
                     AKAIVDG"
BASE COUNT          249 a          433 c          429 g          239 t
ORIGIN      
        1 accgtcgaca atagccttgg ccagctcgtc ggcccggacc gcctcgacga ggctcctcgt
       61 cgacagcttg atcccgtctt cgcgtagcga gtacgacagg gagacgtcca cctcgtcgac
      121 cgacagcacg aggtcgttgg cggctccgac cagcgagtcg tcggcgtccc gcaggcccag
      181 gaaggcgagc cgaccatgcg tctcgacctt gccgagcgcc tcggcgtaca tcgccagatc
      241 cctgagcgta atctggctgc cgcgtagctt ccgtatcagg tcggcgtccg cccaggcccg
      301 gagctcgtag aacatcctgg cgtccaggtc ggagacgccg cgggtcaggt tatcagtgtc
      361 gacgaagatg ccgtacatga gggccgtggc cacgagcccg ggcggcgcca tgccggcctc
      421 ggcgaagtac tcggctatca tcgcggcgca ggagcccacc tcgggacgta tgtcggcgta
      481 tcggtacgcc gcgtcgtcgc ggagggcgtg gtggtctatc gcggccacat cccggcaggc
      541 gaggtcgacc atgttggcgt tgccgcgttg cccgtcgatg atgacggccc agtcgtccgg
      601 gcctatcgtc ggcacgtccg aggccaggga cagctcgatg cccaggagct ccaccatgcg
      661 ctgggcgtcg atcttctcga attctcgctc gtagacgatg gtcgactcga cgccgtagct
      721 cgacagcagg tggcggaggc cgaaggcgga cgcgatggcg tcggggtcgg gcacgtcgtg
      781 ggcctggatg aacaccgacc ccggcgcggc ccgcagtatc gaagcgagcg tctctatcct
      841 cgttgccatg catgcctccg tcgctccgag cgtccctagc cacagatgct accaacgatg
      901 cccccaacgc gcaagacagg tacgagcgat acgagggaat ttgatccacg gaggcacaga
      961 gcaacggagc gggctacgaa ggaccggccc cggaacgaca gaatatgatg agctggatgg
     1021 cgtatgatga ccaggatggc gtctaggtgc tttgcttacc gtacaaacgg cagcctattt
     1081 tatcgagaaa atactggaca tcatgttccg ccgttcgtcg tatacgcctt cccgtttgta
     1141 tacgaatagt ctcttcgttc agttagcctt attcgcccca gcgcgcgtcg cggcccttgc
     1201 cgcgcattcg gcgcagtggc tataatcccc gcgcgccaca cgagtattct acggaggaac
     1261 ggcatgaatc gagccctgga aaccctgaag gaacgcggct tcgtacagca atgcaccgac
     1321 atcgaggggc tctccgccct gatggaccag
//
LOCUS       DAOOLN010000691         1349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_130475_length_1349_cov_2.700927, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000691 DAOOLN010000000
VERSION     DAOOLN010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130475_length_1349_cov_2.700927"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..513)
                     /locus_tag="PLW80_08785"
     CDS             complement(175..513)
                     /locus_tag="PLW80_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66646.1"
                     /translation="MDERLREVFPDGGLIKTVDEPNGELSAEQKAHLNRRGNMLYNGG
                     DLETARRIFQTTGYSDGLIRVGESYMDAGRPVDALKMFKLAHDQARSAELIERAAMAI
                     RKMLDEKDTE"
     gene            complement(862..>1349)
                     /locus_tag="PLW80_08790"
     CDS             complement(862..>1349)
                     /locus_tag="PLW80_08790"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HPB66647.1"
                     /translation="DRMRESIFSIVGPLDGLSFLDLFSGSGIIALEAASRGASPVTCV
                     ERDRGKLGLLIKNVSIAPERVECRCVPVERFILRCERRYSVVFCDPPFPYAHKADIVR
                     SIAERGVVEPGGLVMIHYPAEESMPESHAGLALDDERAYGRSTVRFYRMAGAIDPTTS
                     V"
BASE COUNT          243 a          409 c          409 g          288 t
ORIGIN      
        1 ccgggtcctc ctcgaggatc ttcctgaaat tgcgctccgc gtcgcttatc cggttctcct
       61 tgaggtactg gtatcccttt ttggacagtt ccgcgatttc ggcgagcgcg ttgttctgct
      121 ggggggccgt cccggcgtcg aactcgaact cgtcatgttg gttggtttgg tcgctcattc
      181 tgtatccttc tcgtctagca tttttcttat cgccatagcc gctctctcga tcagctcggc
      241 gcttcgcgct tggtcatggg ctagcttgaa catcttcaac gcgtcgactg gccgaccggc
      301 gtccatgtag gactcgccga cgcgaataag gccgtcggag taaccggtcg tctggaagat
      361 gcggcgggcc gtctccaggt ctccaccatt gtagagcata ttcccgcgcc ggttaagatg
      421 agccttctgt tcggccgata gctcgccgtt aggctcgtcg accgtcttta tcagtccgcc
      481 gtcggggaaa acctcacgta aacgctcgtc catcgggact ccgtgaccgg cgaagcgccg
      541 agaggcgcgc cggttgtttt ggtattgaag aaaatatagt tgacttcata taaataatca
      601 attcacttgc gaggatacgg tcttgatgaa aaataaacgg cgcgtgatat agttttttta
      661 gtaaccggat gaatcatcat gcggggcggc cgtaacgccc cccgggacgg gagcgcccga
      721 tgctatgtga cgaactcggt ctctacgcgg ctgactcgat gtacgccgag gactccgaac
      781 aggctctgcc tccctccgag gctcgtgtcg tggacgagcc catcggaacg gacctgtccg
      841 cgctggatcg caggctgatc gctatactga ggtcgttgga tcgatagccc ccgccatacg
      901 gtagaaacgc accgtcgacc gcccgtaggc gcgctcgtca tcgagcgcca gccccgcgtg
      961 cgactccggc atgctttcct cggccgggta gtggatcatc acgagcccgc ccggctcgac
     1021 cacgccgcgc tcggctatgg aacgcacgat gtcggcctta tgggcgtacg ggaagggcgg
     1081 gtcgcagaat acgaccgagt agcgcctctc gcagcgcagg atgaatcgct cgacgggcac
     1141 gcaccggcat tcgacccgct ccggggctat cgagacgttc ttgatgagta agccgagctt
     1201 gccccggtcc cgctcgacgc aggtgacagg ggacgcccct cggctggcgg cctcgagggc
     1261 gatgatgccg gaacccgaga acaggtcgag gaacgacagc ccgtcgagcg ggccgacgat
     1321 ggagaagatc gattcgcgca tcctgtcca
//
LOCUS       DAOOLN010000692         1347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_130785_length_1347_cov_2.712074, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000692 DAOOLN010000000
VERSION     DAOOLN010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130785_length_1347_cov_2.712074"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..927
                     /locus_tag="PLW80_08795"
     CDS             61..927
                     /locus_tag="PLW80_08795"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB66648.1"
                     /translation="MPCGLLDDPYLVAGFIIASVFFDGVTDPARTAMTMDVTTPENRQ
                     GAFSLIYLGHNLGFAIGQIIAGFLFASAPSWMFWGNAMAVGASLVLVGLFLPESRPSA
                     EEEQASLDTDSCEKAHEGSLWSALRSRPTLLVFAALTTFYGFSYAQHRFAMPLQATSL
                     FGDSGARLYGFMMTLNAVCVVALTTPLVALTRRVRPVFNVALAGALYAVGFGMLALAR
                     SPWLFFASTVLWTVGEIVQATNESVYVSNHTPMSHRGRFNAVLPLISGLGFSLSTPIA
                     GRIADRSGLSAV"
     gene            complement(1089..>1347)
                     /locus_tag="PLW80_08800"
     CDS             complement(1089..>1347)
                     /locus_tag="PLW80_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66649.1"
                     /translation="TGHARVALANARARFELNPVEEDIIVSHMWPIGKPFYSYSESAL
                     VSLVDKYVSTMEVALLFVRTLRSFGYAVAQHLKARLIASRA"
BASE COUNT          197 a          441 c          455 g          254 t
ORIGIN      
        1 gaccgctttg gccggaagcg cgtgcagatg gcgtcgtagc tgctctgcgg ggccctattc
       61 attccctgcg gcttgctcga cgacccgtac ctggtcgccg gcttcatcat cgcgtcggta
      121 ttcttcgacg gcgtcaccga cccggcgcgc acggcgatga ccatggacgt gacgacgccc
      181 gagaaccggc aaggcgcgtt cagcctgatc tacctcggcc ataacctggg cttcgcgata
      241 ggccagatca tcgccggatt tctattcgcg tcggcgccgt catggatgtt ctggggcaac
      301 gcgatggccg tcggggcgtc cctcgtgctc gtcggcctgt tcctgccgga gtcgaggccg
      361 agtgccgagg aagagcaggc gagcctggat actgactcgt gcgagaaagc ccacgagggt
      421 agcctctggt cggcgctacg ctcgaggcct accctcctcg tcttcgccgc gctgacgacc
      481 ttctacggct tttcgtacgc gcagcaccgc ttcgcgatgc cgctccaggc gacgagcctg
      541 ttcggcgact cgggcgccag gctgtacggc ttcatgatga cgttgaacgc cgtctgcgtg
      601 gtcgcgctga cgacgccgct cgtcgccctg acgcggcgag tacgcccggt cttcaacgtg
      661 gcgctagccg gcgccctgta cgccgtgggc ttcggcatgc tcgccttagc ccgctcgccg
      721 tggctgttct tcgcgtcgac cgtactatgg acggtcggcg agatcgtgca ggcgacgaac
      781 gagagcgtct atgtgtcgaa ccatactccg atgtcgcatc gcggccgctt caacgcggtg
      841 ctaccgctga tatccggcct gggcttctcg ctgtccacgc cgatagcggg ccgtatcgcg
      901 gaccgctccg ggctatcggc ggtatgaatc gtcgtggcgg cgtccacggg cctggccgcc
      961 gtcggcgtct ggacgctcgg gcgctacgag aagcgtacag ctccggccgg ccgagccgga
     1021 acgggtgacg aggcgagcgt ctgaggatgc ccggttccga ggctcggcgt acgcgccgcg
     1081 gcgagcctct acgctcttga ggctatcaag cgtgccttga ggtgctgcgc tacggcgtag
     1141 ccgaaggatc gcagggttcg gacgaacaac agggcgacct ccatcgtcga cacgtacttg
     1201 tcgacgaggc tgacgagggc cgactcgctg tacgagtaga atggcttgcc tatgggccac
     1261 atgtgcgaga cgatgatgtc ctcctcgacc gggttgagct cgaagcgggc cctggcgttg
     1321 gccaacgcca cgcgcgcgtg gccggtc
//
LOCUS       DAOOLN010000693         1341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_131695_length_1341_cov_2.993002, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000693 DAOOLN010000000
VERSION     DAOOLN010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131695_length_1341_cov_2.993002"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PLW80_08805"
     CDS             <1..380
                     /locus_tag="PLW80_08805"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HPB66650.1"
                     /translation="AALAAIRDRGLVGRTTVISFDFPTLAAARELEPSIRTCALVGKA
                     WFKAAGESTADQAAAIAASGSDYAGVDHRYLSRELYDALRAAGLGVGAWTVDDPAVMK
                     ALSSLGVDFITTNRPDLLAAALP"
     gene            485..1024
                     /locus_tag="PLW80_08810"
     CDS             485..1024
                     /locus_tag="PLW80_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66651.1"
                     /translation="MQWYVTWVTENLLLSAFIQFALLGTAGEVLGAFASRRRPSGSPV
                     QWLAKAVVWGCLGVLVKTSFTGFKGFLAALVEHGFLPAACEEVPLLRAFCLSALTNSM
                     FGPLLMFLHRSTDNLIARASGYRGIDKSLTTLAWFWLPAHTVTFALPLPFQMGLAALW
                     SVALGIIMGFTKRSAKEAA"
BASE COUNT          162 a          539 c          406 g          234 t
ORIGIN      
        1 ccgccgcttt ggccgcgatt cgcgatcgcg gcctcgtagg ccgcacgaca gtcatatcct
       61 tcgacttccc gaccttggcc gccgcccgcg agctcgagcc gtcgatccgg acctgcgccc
      121 tggtcggcaa ggcctggttc aaggccgctg gcgagtcgac ggccgaccag gccgccgcga
      181 tcgcggcctc gggctccgac tacgcgggcg tcgaccaccg ctacctgtcc cgcgagctgt
      241 acgacgcgct cagggcggcc ggcctcggag tcggggcgtg gactgtggac gacccggcgg
      301 tcatgaaggc cctgtcgtcg ctcggcgtcg acttcatcac gaccaaccgc cccgacctgc
      361 tcgcggccgc cctgccctga ctgtccgcgc gcctactccg cgttgacgac ccgccccgcg
      421 gttcggccga cagaaacgcg tacaccgcta gcgtggcggg ggcgccgagg ctatactccg
      481 cgccatgcag tggtacgtaa cctgggtcac cgagaacctg ctcctctcgg ccttcattca
      541 gttcgcccta ctcggaaccg ccggcgaggt cctcggcgcc ttcgcctcca ggcgcaggcc
      601 gtccggctcg cccgtccagt ggctggccaa ggccgtcgtc tggggctgcc tcggcgtcct
      661 cgtcaagacg tcgttcaccg gcttcaaggg cttcctcgcc gcgctcgtgg agcacggctt
      721 cctgcccgcc gcctgcgagg aggtgcccct ccttcgcgcc ttctgcctgt cggccctgac
      781 caactcgatg ttcggcccgc tcctgatgtt cctgcaccgt agcaccgaca accttatagc
      841 gcgcgccagc ggctaccgcg gcatcgacaa gagcctgacc acgctcgcct ggttctggct
      901 gcccgcccac accgtcacct tcgccctgcc cctgcccttc cagatgggcc tcgcggcgct
      961 ctggtccgtg gccctcggca tcatcatggg cttcaccaag cgatcggcca aggaagcggc
     1021 ctaaggccga cgatagaccg gcccgacagc ggcccgcgtc cgcgggtccg cccggccggc
     1081 cctccggcgt cccgggcccc cgcgcggccg cgctccgcct ctacgcactc gtcggcctcc
     1141 tcacgccgcg ccggccggcc cgtccggctt cgccctcgtc gccgccgccg gcgccggcga
     1201 cctgacgctc tgctcccgcc agcttgatat tatattgtat ttttactata tagcttgcat
     1261 gaactcaaga accacggccc aggccgtcgc gtcgctcgcc ttcgtgctcg cggtggccgt
     1321 cgggggcttc ttctatccgc t
//
LOCUS       DAOOLN010000694         1340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_131881_length_1340_cov_2.919844, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000694 DAOOLN010000000
VERSION     DAOOLN010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131881_length_1340_cov_2.919844"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PLW80_08815"
     CDS             <1..271
                     /locus_tag="PLW80_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66652.1"
                     /translation="VAGVKLKHLKSGAVTALPLFFFAEGVKRVPLSRMGFLQYISPTL
                     QLALGLFVFGETLSATKAAAFAFIMAALVVFAATRPRCTASGESA"
     gene            complement(285..1073)
                     /locus_tag="PLW80_08820"
     CDS             complement(285..1073)
                     /locus_tag="PLW80_08820"
                     /inference="COORDINATES: protein motif:HMM:NF003969.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HPB66653.1"
                     /translation="MDTRLLDGKAARLAVRSGAWTGPTAGLAPGYAQANFVALPSRYA
                     FDFLLFCQRNRKACPVLDVCEAGSTTAARVAADSDVRTDIPRYRVYERGRLVAEPGDL
                     LDDWDDDYVSFLIGCSFTFEGALLAAGLPVRHVEQGRNVPMYRTNRPCAGAGPFSGEL
                     VVSMRPMPGSMVAEAYKVTGRFPEVHGAPVHAGDPEALGIVDIDAPDFGDAVDIRPGE
                     VPVFWACGVTSQAAIMNAALERVVTHAPGHMFITDRRDEELASM"
     gene            complement(1112..>1340)
                     /locus_tag="PLW80_08825"
     CDS             complement(1112..>1340)
                     /locus_tag="PLW80_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66654.1"
                     /translation="RLASVISADLGALAQVPPGGRVRFELVSAAEARSALLERERESE
                     MALISRGPARVARRFAVTVDGERFDVAVEPR"
BASE COUNT          220 a          463 c          440 g          217 t
ORIGIN      
        1 cgtggccggc gtcaagttga agcatctcaa gtccggggcg gtgaccgccc tgccgctgtt
       61 cttcttcgcc gagggagtca agcgcgtgcc gctatcgagg atgggattcc tccagtacat
      121 atcgccgacc ctgcagctgg cgctcggcct gttcgtcttc ggggagacgc tatcggcgac
      181 caaggcggcc gccttcgcgt tcatcatggc ggctctggtc gtgttcgccg cgacgaggcc
      241 gaggtgcacc gctagcggtg agtcggccta ggcgccttgc gggcttacat cgacgccagc
      301 tcctcgtcgc gcctatcggt gatgaacatg tggcctggcg cgtgggtcac cacccgctcg
      361 agggcggcgt tcatgatggc ggcctgggac gtaacgccgc aggcccagaa taccggtacc
      421 tcacctggcc gtatatccac ggcgtctccg aaatcaggcg catctatatc cactatcccg
      481 agcgcttccg ggtcgccggc gtgcaccggc gcgccgtgca cctcggggaa gcgccccgtg
      541 accttgtagg cctcggcgac catcgaaccg ggcatcggcc tcatgctgac gaccagttca
      601 ccggagaaag ggcccgctcc agcgcaggga cggttcgtgc ggtacatcgg cacgttcctg
      661 ccctgctcga cgtgccgtac cggcaagcca gcggcgagaa gcgcaccctc gaacgtgaag
      721 ctgcacccta tgaggaacga cacgtagtcg tcgtcccaat cgtcgagcag gtcgcccggc
      781 tcggcgacga ggcggccgcg ctcatagacc cggtagcgcg ggatatcggt acgcacgtcc
      841 gagtccgcgg ccacccgcgc ggccgtcgtc gacccggcct cgcagacgtc aagcaccggg
      901 caggccttgc ggttacgctg gcagaatagc aggaagtcga aggcgtaacg cgaaggcaac
      961 gcgacgaaat tcgcctgggc gtagccgggg gccaggccgg ccgtcggccc agtccatgcg
     1021 cccgagcgca cggccaggcg agccgccttt ccatccaaga gcctcgtatc catgatgatc
     1081 ctccattttt ctcgtgaact cggggagcgg atcaacgcgg ctcgacggcg acgtcgaaac
     1141 gctctccgtc gacggtcacc gcgaagcgac gggcgacgcg agctggcccg cggctgatga
     1201 gcgccatctc tgactcgcgt tcccgctcga gtagcgcgga cctcgcttcg gcggctgata
     1261 ctagctcaaa gcgtacgcgc ccacccggcg gcacctgggc cagcgcgccg aggtcggcgc
     1321 tgatcacgct ggccagcctc
//
LOCUS       DAOOLN010000695         1338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_132153_length_1338_cov_3.497272, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000695 DAOOLN010000000
VERSION     DAOOLN010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132153_length_1338_cov_3.497272"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1328)
                     /locus_tag="PLW80_08830"
     CDS             complement(3..1328)
                     /locus_tag="PLW80_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HPB66655.1"
                     /translation="MPWLAAQGVAVPEVYLPAAGADYADWAVVACDQYSSEREYWEDV
                     SRLVGGKPSTLNLIFPECYLEDDDKAARVERIRSAMRSYLESGKVAKQAPGFVLVERA
                     TPFEQHPRKGLVIAVDLEAYQYGKGVTSRIRPTEGTIVERLPPRMDIRRGAPLELPHI
                     MLLIDDPKRSVIEPLYAKMDSLPKLYDFDLMKGAGHIRGAMVSDEADLAGVATALEAI
                     ADEGAYRAKYGSSDVLLFAVGDGNHSLATAKAIWEEIKKANASDPELMSHPARYALVE
                     LVNIYDEGLPFHPIHRVLFKADERALLADLAAAGAVFEPMDWKKAVAKTDEPSGAEHR
                     FAFVSAGTSGLVRFAKPSANLAAGTIQEVLDAYLKAHAGTMIDYIHGTDSLETLARKP
                     GNLGLYLPPIDKSSFFGTVIRDGAMPRKTFSMGEASEKRFYIEARKITR"
BASE COUNT          203 a          413 c          486 g          236 t
ORIGIN      
        1 cgtcagcggg ttatcttcct ggcctcgata tagaagcgct tctccgaagc ctcgcccatc
       61 gagaaggtct tgcggggcat ggcgccgtcg cggatgacgg tgccgaagaa gctcgactta
      121 tcgatcggcg gcaggtagag accgaggttg ccgggcttcc gggccagggt ctcgaggctg
      181 tcggtgccgt ggatatagtc gatcatggtg ccggcatggg ccttgaggta ggcgtccagg
      241 acctcctgga tggtgccggc cgccaggttc gcgctcggct tggcgaagcg gacgaggccg
      301 ctcgtcccgg ccgacacgaa ggcgaagcga tgctcggcgc cggaaggctc gtcggtcttg
      361 gctacggcct tcttccaatc catcggctcg aaaacggcgc cggcggcggc gaggtcggcg
      421 aggagagccc gttcatcggc cttgaacagg acgcggtgga tggggtggaa cggcaggccc
      481 tcgtcgtaga tgttgacgag ttcgacgagg gcgtagcgcg ccggatggct catcagctcg
      541 gggtcggagg cgttagcctt cttgatctcc tcccagatgg ccttggcggt ggccagcgaa
      601 tggttgccgt cgccgacggc gaacaggagc acgtccgatg agccgtactt ggcccggtac
      661 gcgccctcgt cggctatcgc ctcgagagcc gtggccacgc cggccaggtc ggcctcgtcg
      721 gagaccatcg cgccgcggat atggccggcg cccttcatga ggtcgaagtc gtagagcttg
      781 ggcaagctgt ccatcttggc gtacagcggc tcgatcacgc tacgcttggg gtcatcgatg
      841 aggagcatga tatggggtag ctcgagcggc gcgccgcggc gtatgtccat gcgcgggggc
      901 aggcgctcga cgatggtgcc ctcggtcggc cgtatccggc tcgtgacgcc cttgccgtac
      961 tggtaggcct cgaggtcgac ggcgatgacg aggcccttgc ggggatgctg ctcgaacggg
     1021 gtcgcgcgct cgacgagcac gaaaccgggc gcctgcttgg ctaccttgcc cgactcgagg
     1081 taggagcgca tcgcggagcg gatgcgctcg acgcgggccg ccttgtcgtc gtcctcgagg
     1141 tagcactcgg gaaagatcag gttgagggtg ctcggcttac cgccgaccag cctggagacg
     1201 tcctcccagt actcgcgctc gctcgagtac tgatcgcagg cgacgacggc ccagtcggcg
     1261 tagtcggcgc ccgcggcggg caggtatacc tctggcacgg cgactccctg cgccgccagc
     1321 cacggcagta cacccttg
//
LOCUS       DAOOLN010000696         1338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_132161_length_1338_cov_3.176150, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000696 DAOOLN010000000
VERSION     DAOOLN010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132161_length_1338_cov_3.176150"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLW80_08835"
     CDS             <1..294
                     /locus_tag="PLW80_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPB66656.1"
                     /translation="IIFKNLLPYLVSVIMLQTALSIPGTIGWEVFLTYIGLGLPVSIP
                     SLGNLVNEGRQIMMSPELRYQLLFPTVVLSMITISFYVLGNAFSDASDPRNHV"
     gene            306..>1338
                     /locus_tag="PLW80_08840"
     CDS             306..>1338
                     /locus_tag="PLW80_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB66657.1"
                     /translation="MANGPAILEAKGLVIKFNLRGRTLTAIRGAGLELYEGETLAIVG
                     ESGSGKSVLTKSLIGMLDRNGWVDSGSAMFEGRDLARIKEERDWLGVRGKKISMVFQD
                     PMTALNPLLTIGAQIREVFELHHGLSGKKADHAVVEMLERVGIDNPEERRRQYPHEFS
                     GGMRQRVVIAIAVACKPRILICDEPTTALDVTIQAQILGLIKALQRDLAFTTVYITHD
                     LGVVANVADRVAVMYAGRIVETGLAEEVFYDSRHPYTWALLSSLPQLGVKGRPLYSIS
                     GTPPNLFNEVRGDAFAPRNPKALKVDFVAEPPEFEVSPTHRASTWLLDPRAPRIEPPP
                     AILAMRAKLG"
BASE COUNT          237 a          449 c          432 g          220 t
ORIGIN      
        1 atcatcttca agaacctgct accctacctg gtatcggtga tcatgctgca gaccgccctg
       61 tcgataccgg gtaccatagg ctgggaggta ttcctgacct acataggcct gggcctgccg
      121 gtcagcatcc cgtcgctcgg caacctggtc aacgaggggc ggcagatcat gatgtccccg
      181 gagctgcgct accagctgct gttcccgacg gtcgtgctgt cgatgataac catctcgttc
      241 tacgtgctcg gcaacgcgtt ctccgacgcg tccgacccgc gtaaccacgt gtaaggacgg
      301 cggacatggc gaacggaccg gcgatactcg aggccaaggg cctcgtcatc aagttcaacc
      361 tgcgcggccg caccctgacc gccatccggg gcgcgggcct cgagctgtac gagggcgaaa
      421 cgctcgcgat cgtcggggag tctgggtccg gcaagtcggt gctgaccaag tcgctcatcg
      481 gcatgctcga ccgaaacggc tgggtcgact cgggctcggc gatgttcgag ggccgcgacc
      541 tggcgaggat caaggaagag cgcgactggc tcggcgtgcg tggcaagaaa atatcgatgg
      601 tgttccagga tccgatgacg gcgctcaacc cgctcctgac gataggcgcc cagatccgcg
      661 aggtcttcga gctgcaccac gggctgtccg gcaagaaggc cgaccacgcc gtcgtcgaga
      721 tgctcgagcg cgtaggcatc gataatcccg aggagcggcg caggcagtac ccccacgaat
      781 tctccggcgg catgcgccag cgcgtggtca tagcgatagc ggtagcctgc aagccgcgca
      841 tcctgatctg cgacgagccg acgaccgcgc tcgacgtgac catccaggcc cagatcctcg
      901 gcctgatcaa ggccctgcag cgcgacctgg ccttcacgac ggtctacatc acccacgacc
      961 tcggcgtggt ggccaacgtg gccgaccggg tcgcggtgat gtacgcgggt aggatcgtcg
     1021 agaccgggct ggccgaggag gtgttctacg attccaggca cccgtatacg tgggccctcc
     1081 tgtcgtcgct gccgcagctc ggcgtcaagg gccggccgct gtactcgata tccgggacgc
     1141 cgcccaacct gttcaacgag gtcaggggcg atgcgttcgc gccgcgcaac cccaaggcgc
     1201 tcaaggtcga cttcgtcgcc gagccgccgg aattcgaggt gagcccgacc catcgggcgt
     1261 ccacctggct gctcgacccg cgcgcgccga ggattgagcc gcccccggcc atcctcgcga
     1321 tgcgcgccaa actcggcg
//
LOCUS       DAOOLN010000697         1338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_132186_length_1338_cov_2.713172, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000697 DAOOLN010000000
VERSION     DAOOLN010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132186_length_1338_cov_2.713172"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PLW80_08845"
     CDS             complement(<1..302)
                     /locus_tag="PLW80_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66658.1"
                     /translation="MAMPTPYAKPVTIASPDVGLREPSVSRSALGLARSVLGTYLRLA
                     LGFERIELRGGDELARAFAEALSGERRLILAFRHPYGDEPQLLSWAFLRGVELEARR"
     gene            430..948
                     /gene="tpx"
                     /locus_tag="PLW80_08850"
     CDS             430..948
                     /gene="tpx"
                     /locus_tag="PLW80_08850"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HPB66659.1"
                     /translation="MATITLGGNPVHSSGELPTIGARLPDFRLTRSDLSDVSLADFKG
                     KVLVINIVPSLDTGVCAASARAFNMRIKESAGALVLTVSRDLPFAQRRFCEAEGIDAV
                     ITLSELRDRSFGKDYGVELIDGPMAGLLSRAVVVADATGRVVYVEQVPEIKREPDYGK
                     ALDAVRAALAGY"
     gene            1024..1233
                     /locus_tag="PLW80_08855"
     CDS             1024..1233
                     /locus_tag="PLW80_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HPB66660.1"
                     /translation="MKKILTIEGMSCGHCAMRVKKALEEVAGVSSATVDISEGKAVVE
                     GEAPDPNMLRDAVEKAGYVVTGVLP"
BASE COUNT          262 a          411 c          437 g          228 t
ORIGIN      
        1 cgtcgggcct cgagctcgac gccgcgcagg aaggcccagg acaggagctg cggctcgtca
       61 ccgtagggat ggcggaacgc cagtatcagc cgacgctcgc ccgacaacgc ctcggcgaag
      121 gccctggcca gctcgtcgcc cccgcgtagc tctatccgct cgaagcccag cgcgagcctc
      181 aggtaggttc cgagaacgga cctggccagc ccgagcgccg atcgcgatac ggacggctcg
      241 cgcaggccca cgtcggggga ggcgatggtg acgggcttgg cgtacggagt gggcatggcc
      301 atgggccagc ataccccgca agtcgtctat cggcaagctc ggatcgaggg cgccagcccc
      361 atacttgatc gcgtaatgaa atagccgtac tttcaagtca agcgctccag cgccataccg
      421 gaggttatca tggctaccat cacgctcggc ggcaacccag tccatagctc gggagaactc
      481 cctacaatag gcgcgaggct cccggacttc aggctcacca gatcagacct ttcggacgta
      541 tcgctagccg acttcaaggg caaggttcta gtgatcaaca tcgtcccgag cctcgatacg
      601 ggagtctgcg cggccagcgc cagggccttc aacatgagga tcaaggagtc ggccggcgcc
      661 ctggtcctga ccgtcagccg cgacttgccg ttcgcgcagc ggcgattctg cgaggccgag
      721 ggcatcgatg ccgtcataac gctgtcggag ctacgcgacc ggtctttcgg gaaggactac
      781 ggcgtcgagc taatagacgg accgatggcc gggctcctat ccagggccgt cgtcgtcgcg
      841 gacgcgacgg gccgcgtggt ctacgtcgag caggttccgg agatcaaacg tgaacccgat
      901 tatggcaagg cactcgacgc ggtccgggcc gcgttggcgg gttactgacc tctacctatt
      961 ctcattgctc cgtagtcgtg atatattata ccctataggg gtattataga cgaaggagag
     1021 cctatgaaga agatactgac catcgagggc atgagctgtg ggcattgcgc catgcgggtc
     1081 aagaaggccc tcgaggaggt cgccggcgtt tcgtccgcga cggtggacat aagcgagggc
     1141 aaggccgtgg tcgagggcga ggcgcctgac ccgaacatgc tgcgcgacgc ggtagagaaa
     1201 gccggctacg tcgtcaccgg cgtgctcccc taggctcgcg gcaccggaat acgtcgaagc
     1261 cccggactcc gctggagccc ggggcttcga cgtaatgagt cgtattactt aagatcggat
     1321 agcctgatcg gtttcggc
//
LOCUS       DAOOLN010000698         1337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_132413_length_1337_cov_2.111544, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000698 DAOOLN010000000
VERSION     DAOOLN010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132413_length_1337_cov_2.111544"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..467)
                     /locus_tag="PLW80_08860"
     CDS             complement(111..467)
                     /locus_tag="PLW80_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66661.1"
                     /translation="MGDYYPVDAGEVRKNGMRGVISTGAGIGLMLFNSLLNIPIVGWI
                     LGGGLVVLGVMGLVGRTRTDKTTGTVMMAAGALGIASFVLKGFTGFILGAAGIGLVGF
                     GVFNLFKFVKGLKSRG"
     gene            complement(594..1334)
                     /gene="folE2"
                     /locus_tag="PLW80_08865"
     CDS             complement(594..1334)
                     /gene="folE2"
                     /locus_tag="PLW80_08865"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HPB66662.1"
                     /translation="MPIRKVGVKGVRYPITVLDRANSIQHTTATVNLFVDLPHHYKGT
                     HMSRFIEVFNEHRRDLSMPRFLAMLDELRDALDAETAYCDMHFPYFIEKKAPVSGQPG
                     MMSYDCSYEGSSSAEEREFWVSVQVPVQTVCPCSKAISEYGAHNQRGLVKVRVKLGPF
                     FWIEDLVAIVERSASSDVYTLLKREDEKYVTERSFERPMFVEDIVREVHGGLTALGIF
                     PKFCVEAENFESIHNHSAYACAEYPLDR"
BASE COUNT          277 a          411 c          403 g          246 t
ORIGIN      
        1 gttcatctgg caaccgtagg tctctataaa aaaacgcatg atctccccgg atatgaatcg
       61 ggcggccgac tgagcggccg cccacgttga ggtcagagca tcgacggagc ttagcccctg
      121 gacttgagcc ccttgacgaa tttgaacaag ttaaacacgc cgaagcccac caagccgatg
      181 ccggctgcgc cgaggatgaa gccggtgaag cccttgagca cgaacgaggc tatgccgagc
      241 gcaccggcgg ccatcatgac cgtgccggtg gtcttatcgg tacgcgtccg gccgacgaga
      301 cccatgaccc cgagcacgac gagtccgccg ccaaggatcc atccgacgat tggtatgttg
      361 agcaatgaat tgaacagcat caagccgatc ccagcgcccg tcgagatgac gccgcgcata
      421 ccgttcttgc ggacctcgcc cgcgtcgacc ggatagtaat cgcccataga gtcctcctta
      481 atgatcggcc gtcacctcgt cgctcccata cggcgagcgg cttcgcgttg ccgcgacggc
      541 ggcgccaata agcaatatac ccaaaagctg acggaccgta aaggaggcga aagctaccgg
      601 tcgagcggat actcggcgca cgcgtacgcc gagtgattgt gaatgctctc gaaattctcg
      661 gcctcgacgc agaatttcgg gaaaatgccg agggccgtaa ggccgccgtg cacctcgcgt
      721 acgatatcct ccacgaacat cggccgttcg aacgaccgct cggtcacgta cttctcgtcc
      781 tctcgcttga ggagcgtgta cacgtcgctg gacgcggacc gctcgactat ggccaccagg
      841 tcctctatcc agaaaaaagg cccgagcttg acgcggacct taacgaggcc tcgctggtta
      901 tgggctccgt actcgctgat cgccttggaa caggggcaga cggtctgtac cggcacctga
      961 acggagaccc agaattcccg ctcctcggcc gacgacgagc cttcgtagga acagtcgtag
     1021 ctcatcatgc cgggctgccc ggataccggc gccttcttct cgatgaaata cgggaaatgc
     1081 atgtcgcagt acgcggtctc cgcgtcgagg gcgtcgcgta gctcgtcgag catggccagg
     1141 aagcggggca tggacaggtc gcgcctgtgc tcgttgaaca cctctatgaa gcggctcata
     1201 tgggtgccct tgtagtggtg cggcaggtcg acgaacaggt tgaccgtggc ggtcgtatgc
     1261 tggatcgagt tggcccggtc cagcacggtg atcgggtatc ggacgccctt gactccgacc
     1321 ttgcgtatgg ggatgca
//
LOCUS       DAOOLN010000699         1335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_132631_length_1335_cov_4.386719, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000699 DAOOLN010000000
VERSION     DAOOLN010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132631_length_1335_cov_4.386719"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1335
                     /locus_tag="PLW80_08870"
     CDS             <1..>1335
                     /locus_tag="PLW80_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66663.1"
                     /translation="LRPLLEQANAGDAKDFERRLTLLRNAAEFQVYGFLEAVATMETD
                     GAFLAAARVLESGGDRAYIAPLAARWFERMGSPPYPADKTAVYRAVLDAVKARGDRAA
                     CEAVARELEARRGDRELAAAVLGATDGSQDRVIFDTLCRLFADPLFPLRDELREALLK
                     QPPELVVPFTLGFLRADRSGTPRALRRDALILAGQLKLAWAIQRTMETLPTLDLEEIG
                     ALAPIVVDADSKAFKRKARYILDGVDAPSRASVLAALQAAGDRSFMADIKAALADADP
                     DVRSAAVRALAAFNEDKTLGSGGIELLRDPVERVRVAAAAAIAEAGGPSAVKKLAVVL
                     ADQNEVDEVKLSLVRGLGRATDAASLELLIDALGAKDGRSDELVEALSRRRSRKDLGR
                     ILERFKDATGELKARIAEAVRRMGKPGEEALVDLLAEDIVSLKPFIVEALESL"
BASE COUNT          193 a          504 c          449 g          189 t
ORIGIN      
        1 gctccgcccg ttgctcgagc aggcgaacgc cggcgacgcg aaggacttcg agcgccgcct
       61 gaccctgctg cgcaacgcgg ccgagttcca ggtctacggc ttcctcgagg ccgtcgcgac
      121 gatggagacc gacggcgcct tcctggcggc ggcccgcgtg ctcgagtcag gcggcgaccg
      181 cgcctatatt gcgcccctcg cggcgcgctg gttcgagcgc atgggaagcc ctccctaccc
      241 ggccgacaag actgccgtct accgcgccgt gctcgacgcc gtcaaggccc gaggcgaccg
      301 cgcagcctgc gaggccgtag cgcgggagct cgaggccagg cgcggcgacc gggagctggc
      361 ggccgccgtc ctaggcgcca cggacggctc gcaggaccga gtcatcttcg atacgctgtg
      421 ccggctgttc gccgacccgc tcttcccgct gcgcgacgag ctgcgcgagg cgctgctcaa
      481 acagccgccc gagctggtcg tgccgttcac gctcggcttc ctgcgcgccg accggtccgg
      541 gacccctcgc gcgctccggc gggacgcgct gattctggcc ggtcagctca agctggcctg
      601 ggccatccag cgcacgatgg agaccctgcc gaccctcgac ctcgaggaga taggcgccct
      661 ggcgcccatc gtcgtcgacg ccgattccaa ggcctttaag cgcaaggcgc gttacatcct
      721 cgacggcgtc gacgcgccat cccgggcctc ggtgctcgcg gccctacagg cggccggaga
      781 ccgctccttc atggcggaca tcaaagccgc cctggccgac gccgacccgg acgttcgctc
      841 ggccgccgtc cgcgccctgg ccgcgttcaa cgaggacaag actctcggtt ccggcggtat
      901 cgaacttctc cgcgatccgg tcgagcgcgt acgcgtcgcg gccgcggccg ccatagccga
      961 ggccggcggc cccagcgccg tcaagaagct cgcggtcgtg ctcgccgacc agaacgaggt
     1021 cgacgaggtc aagctgtccc tcgtgcgcgg cctcggccgc gccaccgacg ccgcgagcct
     1081 cgaactcctg atcgacgccc ttggcgcgaa agatggtcgc tcggacgagc tcgtcgaggc
     1141 gctatcgcgc cgccgctccc gcaaggacct cggcaggata ctcgagcgct tcaaggacgc
     1201 gaccggagag ctcaaggcca ggatagccga ggccgtccgc cggatgggca agcctggcga
     1261 ggaggccctc gtcgacctct tagccgagga catcgtctcg ctcaagccct tcatcgtcga
     1321 ggcgctggag tcgct
//
LOCUS       DAOOLN010000700         1335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_132699_length_1335_cov_2.436719, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000700 DAOOLN010000000
VERSION     DAOOLN010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132699_length_1335_cov_2.436719"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PLW80_08875"
     CDS             complement(<1..554)
                     /locus_tag="PLW80_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66664.1"
                     /translation="MKADMKGVREEIQKVGSSMETVAKTMQTSMSAVIRDVTAQMNGL
                     GQATARVAEMLEALQDKPRAAAVAEDARKAYQYQKMKGVQAAQTDPMKRAAASSTERI
                     GLGGFQDATRIMQDFAALSSTLSSKIKGSGIMRSDQLAAQAMRAMTDVDKQIGSAFFG
                     FDFNKIAAEAGASPKKAQKRATQA"
     gene            complement(587..892)
                     /locus_tag="PLW80_08880"
     CDS             complement(587..892)
                     /locus_tag="PLW80_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66665.1"
                     /translation="MDGVVRFFSEEERQELERIATIVFDGGKVPARFAEASEKWADAI
                     SRFLMAVNRENGSMSLNPFDEYPAKTFLVMAFLQNLMCARIKKKMDALSKKSRGRGK"
     gene            complement(844..>1335)
                     /locus_tag="PLW80_08885"
     CDS             complement(844..>1335)
                     /locus_tag="PLW80_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66666.1"
                     /translation="SRAKEYKVTVDEVKAYADKYDVSIADAREMLIDSKAERDAQKAY
                     DKYMEDERIKEYVIMREASTTESRSVQVNDVDDKVLARLPPEAQVERAERIIKQREEK
                     ENEVIDLVAKCIVGSSFVDNGKPADLDKVREIIKSSTFAMSYLVGEWMGSSGSFQKKS
                     GRN"
BASE COUNT          233 a          425 c          344 g          333 t
ORIGIN      
        1 aaggcctgcg tcgctcgctt ctgcgccttc ttcggggacg cgccagcttc cgccgcaatc
       61 ttgttgaaat cgaacccgaa gaaagcgcta ccaatctgct tgtctacgtc ggtcatcgct
      121 cgcatggcct gggcggcaag ctggtccgag cgcatgatcc ccgagccctt tatcttcgat
      181 gaaagagtcg acgacagggc ggcgaagtcc tgcattatcc tggtagcgtc ctggaacccg
      241 ccaaggccaa tccgttccgt ggaagatgcc gccgctcgct tcatgggatc ggtctgcgcg
      301 gcctggacgc ccttcatttt ctggtattga tatgcttttc tggcatcctc ggcaacggcg
      361 gcggcacgcg gcttgtcctg cagcgcctcg agcatttcag caactcgcgc ggtagcttgc
      421 ccaagcccgt tcatctgagc ggttacgtct cggatgacgg cggacattga cgtctgcatg
      481 gtcttggcga cggtctccat agaggagccg accttctgga tctcttcgcg gacgcccttc
      541 atgtccgcct tcatctcgag aaccatagcg agcttgctgc tgctgctcat ttcccgcgcc
      601 ccctagactt cttactcaag gcatccatct tcttctttat tctagcacac atcaggtttt
      661 ggagaaacgc cataacaaga aaggtcttgg ccgggtattc atcgaacggg ttgagcgaca
      721 tggacccgtt ctccctgttt actgccatca agaacctaga tatagcatcg gcccacttct
      781 cagatgcctc tgcgaagcgg gccggtactt tccccccatc aaatacgatg gtggcgatgc
      841 gctctagttc ctgccgctct tcttctgaaa agaaccggac gaccccatcc attcgccgac
      901 gaggtaggac attgcgaaag tagagctctt gattatctcg cggaccttat ccaggtcggc
      961 gggcttcccg ttgtcaacga atgacgagcc tacgatgcac ttggccacga ggtcgatcac
     1021 ctcgttctcc ttctcctcgc gctgcttgat gattctctcg gcacgctcca cctgcgcctc
     1081 gggtggcagt cgtgccagga ccttgtcgtc cacgtcgttg acctgaaccg agcgcgactc
     1141 ggtcgtggaa gcctcgcgca ttatgacgta ctccttgatg cgctcgtctt ccatgtactt
     1201 gtcgtacgct ttctgcgcgt ctcgctcggc cttgctgtcg atcagcattt ccctggcatc
     1261 ggcaatgctc acgtcgtact tatccgcgta agccttgacc tcgtcgaccg tcaccttgta
     1321 ttccttcgcc cgaga
//
LOCUS       DAOOLN010000701         1335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_132719_length_1335_cov_2.173437, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000701 DAOOLN010000000
VERSION     DAOOLN010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132719_length_1335_cov_2.173437"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            410..>1335
                     /locus_tag="PLW80_08890"
     CDS             410..>1335
                     /locus_tag="PLW80_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPB66667.1"
                     /translation="MSCDWLISAVVMGVNGKAVNVLAEVTSTTDEYWKGESSSGAAVT
                     LQGATITFTSLKEGDSTTYSTSVDSSGNWYLSGVSAGKYRITGSQTGWTFIPMDIEIS
                     GFMSEVTTDVLAYPTPTGNPLMIIVRWNNELIDIDAHTIIDLDTDLTNYTGGDYVYHA
                     KKTNTLPAAGTVVLDRDVKFVTSTSSTLNGYPVETVRVISNPFGSGFTGQLRYYLQSY
                     SYYNGLSQVTSNTTLTGDPTATYTGRADATVYVMLGTEHYGTWTMPIDTAEKTLGILK
                     IDVSGTSTATNYEIKSFGNEGNTPKSIGAVSLS"
BASE COUNT          280 a          412 c          369 g          274 t
ORIGIN      
        1 cttgaggctc tcgatgcccg gccccatgtc ggccgacgcc accgtcgacg cggccccgac
       61 cgcggcctgg gtcacggtct gctgtccgaa cgccgccgcg gccaacgcca gcaacgctaa
      121 cgataccaga acgattcgtt tcatgagtcc ctcctcatac cattacatct cggccctacg
      181 ggcgcggact caagcctagc gccggcgctc ggcgaatgca aattttctcg cccgatcggg
      241 ggcttgttga ttttcgccgg aaaggtcata tacttacaga tgcttcacga cggcccgcgc
      301 taggagcgag gcgatcggaa gcctgttcgt agtccataag gaggatcgaa tgaagaagat
      361 agtacgtatc ggagcgatcg cgctcatcgc gttcgcggcc atcggagtca tgagctgcga
      421 ctggctgatc agcgccgtgg tcatgggcgt caatggcaag gccgtgaacg tgctggcaga
      481 ggttacctcg accaccgacg aatattggaa gggcgaaagc tcgagcggcg ccgccgtcac
      541 gctccagggc gcgacgataa cgttcacgag cctcaaggag ggcgactcga ccacgtattc
      601 gacttcggtc gactccagcg gtaattggta tctctctggc gtcagcgccg ggaaataccg
      661 catcaccggt tcgcagaccg gctggacctt catcccgatg gacatcgaga tttccggttt
      721 catgagcgag gtcacgacgg acgtgctggc ctacccgacg cctaccggca acccgctcat
      781 gatcatcgtc cgctggaaca acgagctcat cgatatcgac gctcatacca tcatcgatct
      841 tgatactgac ctgactaatt atactggcgg cgattatgtc tatcatgcta agaagaccaa
      901 tacccttcca gctgccggca cggtcgttct tgatcgcgat gtgaaattcg taacctctac
      961 gagttcgacg ctgaacggct atccggttga gaccgttcgt gtaatttcga atcctttcgg
     1021 ctcgggtttt accgggcagc tccgttatta tctacagtcc tactcgtact acaacggctt
     1081 gtctcaggtg acctcgaata cgacgcttac cggcgatccg accgcgacgt acaccggccg
     1141 tgccgacgcc accgtttacg tgatgctcgg taccgagcac tatggcacct ggaccatgcc
     1201 gatcgacacc gccgagaaga cgctcggtat ccttaagatc gacgtttccg ggacctcaac
     1261 ggcgacgaac tacgagatca agtcgtttgg caacgagggg aataccccga agtccatcgg
     1321 cgccgtgagc ctctc
//
LOCUS       DAOOLN010000702         1333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_132976_length_1333_cov_2.853678, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000702 DAOOLN010000000
VERSION     DAOOLN010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132976_length_1333_cov_2.853678"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="PLW80_08895"
     CDS             complement(<1..748)
                     /locus_tag="PLW80_08895"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate-2-kinase family protein"
                     /protein_id="HPB66668.1"
                     /translation="MSDAMNAEAIFKAAVDRVDPSRMIERAVRIERDDLVVSAGAESV
                     RYGLGGIDRIFVTGMGKASAKMAAGLERALGSRIAGGLVAVKEGHVEPLERVRLVEAG
                     HPVPDGRSQAAAAAILGLGSSLGVRLTERDLVIVLVSGGGSAILCAPAPGLTLEDKIG
                     TTRLLLASGATINEVNCVRKHLSAVKGGRLAAALAPASVLALVLSDVIGDDLDAIASG
                     PTVPDPTSYADALEVVRRYGIESRLPASVAE"
     gene            950..>1333
                     /locus_tag="PLW80_08900"
     CDS             950..>1333
                     /locus_tag="PLW80_08900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPB66669.1"
                     /translation="MTACQFEKIVQASHDFITLIDRDYRYAFVNDSYARELGLPAIDI
                     VGKTVGEIWGDEKFRRRIKPRIDQCFRGDESHDVDKFTFGSDSKYIHVSYYPYGDDGE
                     TSHVMVFSHDISIVKKLESKLLDFEF"
BASE COUNT          235 a          464 c          410 g          224 t
ORIGIN      
        1 gctcggcgac cgacgccggt agcctagact ctattccgta gcgcctgacg acctcgaggg
       61 cgtcggcata gctcgtcgga tccggtaccg tcggcccgga ggctatggcg tcgagatcgt
      121 cgcctatcac gtctgacagg acgagggcga gcaccgaggc cggcgccagg gccgccgcga
      181 gacgcccgcc cttgaccgcc gacaggtgct tgcgcacgca gttgacctcg ttgatcgtcg
      241 cgccggacgc gaggaggagc cgcgtcgttc cgatcttgtc ctcgagcgtc agaccgggag
      301 ccggggcgca caggatcgcc gagccgcctc ccgagaccag gacgatgacg aggtcgcgct
      361 cggtcagcct gacgccgagg ctggagccga ggccgagtat cgcggccgcc gcggcctggg
      421 agcgcccatc ggggacgggg tgccccgcct cgacgaggcg cacgcgctcg agcggctcga
      481 cgtgaccttc ctttaccgcg acgagcccgc ccgctatcct cgagccgagc gcccgctcga
      541 gacccgcggc catcttggcc gacgccttgc ccatgcccgt gacgaagatc cggtcgatgc
      601 cgccgagccc gtagcggacc gactcggcgc cggcggagac gacgagatcg tcccgctcta
      661 tccgtacggc gcgctcgatc atccgcgagg gatccactct gtctaccgcc gccttgaaaa
      721 tcgcctcggc gttcatcgcg tcgctcatgt cgatatcctc gtcatcttgg gttcgagccg
      781 taccgggaac catcgccgcg tcagccatcg ggccgacggg cgccccgccc ctcgctcgat
      841 gataccggat taccgaggat atcgacagcc ctgccatagg ctgatccccg accggtatgg
      901 catttcgcct tcctcgaaac tatacttata gcgccaatcg gggtatagaa tgacagcctg
      961 ccaattcgag aagatcgtcc aggcgtcgca cgacttcatc acgctcatcg accgggacta
     1021 ccgctacgcc ttcgtcaatg actcctacgc ccgcgagctg gggctaccgg cgatcgacat
     1081 cgtcggcaag acggtcggcg agatctgggg cgatgagaaa ttcaggcggc gcatcaagcc
     1141 gcgcatcgat cagtgcttcc gcggcgacga gtcccacgac gtcgacaaat tcaccttcgg
     1201 gagcgactcc aagtatatcc acgtctccta ctacccgtac ggcgatgatg gagagacctc
     1261 gcacgtgatg gtcttctcgc acgacatctc catcgtcaag aagctcgagt cgaagctgct
     1321 cgacttcgag ttc
//
LOCUS       DAOOLN010000703         1332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_133201_length_1332_cov_1.788567, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000703 DAOOLN010000000
VERSION     DAOOLN010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133201_length_1332_cov_1.788567"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="PLW80_08905"
     CDS             complement(<1..1016)
                     /locus_tag="PLW80_08905"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPB66670.1"
                     /translation="MGYVVSQDVLDSDPHFYLGCTDCHDGDSTKATQAEAHKGLVARP
                     SDDPVASCGQCHEETAKTYTLSLHYTAAGMKHGVSPRFDEKGKQLFDTKVFEQSCRSC
                     HASCGDCHVKSPVISGVNTGLLDGHRFVAKAEDKTCALCHGGRVWPEFTGDYGGTPDV
                     HYRKGMTCSDCHGPLQFHGDGNAYASRKDVAVRPLCSDCHDVTTMGKASTKETHATHA
                     DTVSCSACHTASAYRQCADCHVGAGATATPAIILGQNPRKEGQLTTLRLIPTVRDTFK
                     PAGVAMQSFDALPNYWDTVPHNVAKRTDRTRDCNTCHEEGLYYLEAERLPKGGSTKNL
                     ELVP"
     gene            complement(1053..>1332)
                     /locus_tag="PLW80_08910"
     CDS             complement(1053..>1332)
                     /locus_tag="PLW80_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66671.1"
                     /translation="ILLYIYKGITLLYAYSAVYRTAEAVGPHYNSQGGSTVNKATSRR
                     AMLMIAFFGAGALTVHATGSSCLSCHTDPAAMQKLVVPPSGESAEGEG"
BASE COUNT          210 a          405 c          454 g          263 t
ORIGIN      
        1 cagggcacga gttccaggtt cttcgtcgag ccgcccttgg gcagtcgctc ggcctcgagg
       61 tagtacaggc cttcctcgtg gcaggtgttg cagtcgcggg tgcggtcggt gcgcttggct
      121 acattgtggg gcaccgtgtc ccagtagttc ggcagcgcgt cgaagctctg catggctacg
      181 ccggccggct tgaacgtgtc gcgcacggtc ggtatcagcc tgagcgtggt cagctggcct
      241 tctttcctgg ggttctgccc gaggatgata gccggcgtgg cggtcgctcc ggctccgacg
      301 tggcaatcgg cgcactggcg gtaggcgctc gcggtgtggc aggccgagca cgagaccgtg
      361 tcggcgtgcg tcgcgtgggt ctccttggtc gaggccttgc ccatcgtcgt cacatcgtgg
      421 caatccgagc acagcggcct gaccgccaca tccttacggc tcgcgtacgc gttgccgtcg
      481 ccgtgaaact ggagcgggcc gtggcagtcg gagcaggtca tgcccttgcg gtagtgcacg
      541 tcgggagtac cgccgtaatc gccggtgaac tccggccaga cgcggcctcc gtggcacagc
      601 gcgcaggttt tatcctcggc cttggccacg aaacggtggc cgtcgaggag gcccgtgttc
      661 acgccggaga tgacgggact cttgacgtgg cagtcgccgc acgaggcgtg gcaactacgg
      721 cacgactgct cgaagacctt ggtgtcgaac agctgcttgc ccttctcgtc gaagcgcggc
      781 gatacgccgt gcttcatacc ggcggcggta tagtgaaggg acagcgtgta ggtcttggcc
      841 gtctcctcgt ggcactggcc gcaactcgcc acggggtcgt cggacggcct ggctacgagt
      901 cccttgtggg cctccgcctg ggtcgccttg gtcgagtcgc cgtcgtggca gtcggtacag
      961 ccgagataga aatgcgggtc gctatcgaga acgtcctggg atactacgta ccccatgtaa
     1021 taatccgccc gaaccggggg aggagttccc gctcaccctt cgccttcggc ggattcgccc
     1081 gagggcggta cgacgagctt ctgcatggcg gccgggtccg tatggcagga gaggcagctc
     1141 gagcccgtag cgtgtacggt gagcgccccc gcgccgaaga acgcgatcat cagcatggct
     1201 cgcctcgagg tcgccttatt cactgtggaa cctccttgcg agttatagtg cgggccgacg
     1261 gcctcggcgg ttcgatagac cgcactataa gcatacaaga gcgtgattcc cttgtaaata
     1321 tataaaagaa ta
//
LOCUS       DAOOLN010000704         1331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_133341_length_1331_cov_2.218652, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000704 DAOOLN010000000
VERSION     DAOOLN010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133341_length_1331_cov_2.218652"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PLW80_08915"
     CDS             complement(<1..587)
                     /locus_tag="PLW80_08915"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPB66672.1"
                     /translation="MRRSPLAALLLAFMAASAAWAQGGGEQWLDIKEVAARWKASVAY
                     DPLTGTGHVLRGTDLVRFKLDYPVFSVGQGTALRATAIKDEGIRLGIRSDAVRAIESW
                     FAERDAERASHFSVAAILIDPGHGGRDPGAIGEFGSGKDRLRVAEKDVALAIGRDVYD
                     RLVERWPDKRIIITRRGDTFPTLDERVEMANDVQLG"
     gene            complement(590..>1331)
                     /locus_tag="PLW80_08920"
     CDS             complement(590..>1331)
                     /locus_tag="PLW80_08920"
                     /inference="COORDINATES: protein motif:HMM:NF037860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66673.1"
                     /translation="MSGWTYPYSSGLEPTAEAIDIACGVVPAEPGTPLGLSCSERAWI
                     SIPGRVAEIRGLAAAEAMPGVRHVFPRASAGDRVVFPSNNVQKCGNVIAVGGSVAEAD
                     AYAEAAARSILIRLEPDDAATADFLRGNGSLLGPDGSVWPPLAFGGVSAMTESSLHKL
                     PGMVRLNETGGSVSIAPLRGIEAESARDWQGRSISEALEAVAELTGARIGLDGDLVLG
                     SCFWRAFLRGGYQGGAWAVERARLGDRG"
BASE COUNT          207 a          473 c          448 g          203 t
ORIGIN      
        1 ccgagctgta cgtcgttggc catctccacg cgttcatcca gagtcgggaa ggtatctccg
       61 cggcgcgtta tgatgatgcg cttgtccggc caacgctcga ccagccggtc gtagacgtcg
      121 cggccgatgg cgagagcgac gtccttctcg gcgacgcgca acctgtcctt gcccgagccg
      181 aactcgccga tggcgccggg atcccggccg ccatgtccgg ggtcgatcag gatggccgcc
      241 acgctgaaat gcgaggcccg ctcggcgtcc cgctcggcga accaagactc gatagcccgg
      301 accgcgtcgg accgtatgcc tagccgtatg ccctcgtcct tgatggccgt tgctctgagc
      361 gccgttccct ggccgacgga gaagaccgga tagtcgagct tgaagcgtac gaggtccgtg
      421 ccgcgcagga cgtgcccggt gccggtcagg gggtcgtagg ctacgctggc cttccagcgc
      481 gcggcgacct ccttgatgtc gagccactgc tcgccgccac cttgcgccca ggccgccgag
      541 gccgccatga acgccaatag cagcgcggcg agtggcgagc ggcgcatcct catcctcggt
      601 cccccaggcg ggcgcgctcg acggcccagg cgccgccctg gtagccgccg cgtaggaaag
      661 cccgccagaa acacgagccg agcaccaagt caccgtcgag ccctatcctg gcgccggtca
      721 gctccgccac ggcctcgagc gcctcggata tcgagcgacc ctgccagtcc cgggcgctct
      781 cggcctctat gccgcgcagg ggcgctatcg agaccgatcc gcccgtctcg ttcagcctga
      841 ccatgccggg cagcttatgg agcgacgact cggtcatcgc cgatacgccg ccgaaggcca
      901 ggggtggcca gacggagccg tcggggccga ggagcgagcc attccctcgc aggaagtcgg
      961 cggtggccgc gtcatcgggc tcgagccgta tcaggatgga cctggcggcc gcctcggcgt
     1021 aagcgtcggc ctcggcgact gagccgccga ccgctatgac gttgccgcat ttctgaacat
     1081 tattggacgg gaagaccacg cggtcgcctg cggaggccct cgggaagaca tgcctgacgc
     1141 cgggcatcgc ctcggctgcg gccaggcccc tgatctcggc aacgcggcca ggtatcgata
     1201 tccaggcgcg ctccgagcag ctcaggccga gtggcgtccc gggctcggcc ggcacgacgc
     1261 cgcaggcgat gtcgatggcc tcggccgtcg gctcgagtcc gctcgaatac ggataggtcc
     1321 agcctgacat a
//
LOCUS       DAOOLN010000705         1326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_134114_length_1326_cov_2.269079, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000705 DAOOLN010000000
VERSION     DAOOLN010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134114_length_1326_cov_2.269079"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="PLW80_08925"
     CDS             <1..675
                     /locus_tag="PLW80_08925"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPB66674.1"
                     /translation="YAEKADVALSEAEATATGGPEVEAMATGTPVDGAAGVELAEAGR
                     QPAVDAAAGLEPYAEKADVALADASEQKPETAPYVVEPTEEKTPAVALDSPKEYLVAI
                     EPTGPKPPVQTAPAVETKPAGTKVAPAAPAPAASAPAATIVSGALEKGRYYIQIGAYA
                     SEAAAKDAASRLSVGYMVVVQQVTTGGKATWKVFVGPLSRDESGVALVRVRSMGYRDA
                     FVKSGG"
     gene            complement(682..>1326)
                     /locus_tag="PLW80_08930"
     CDS             complement(682..>1326)
                     /locus_tag="PLW80_08930"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB66675.1"
                     /translation="TVSRLFVRLDEEPSAEAAEVEAVVDRSTPLRYPAWLGVFLAYAV
                     MGVVINVFPVFASDELGLSESTIGLALTIRAAATTVGFLALGRLTFWQFKRAAIPALS
                     IAAAATVALLAAARNAAGFALGFTVIGLLQAMVYNNSLFYGVSGASDRDKRASVHESV
                     LTFGQITGSVSGGFVYQALSMPPVFIGLALLLGAGAAAQAVMVYAGHRHPKARV"
BASE COUNT          240 a          440 c          468 g          178 t
ORIGIN      
        1 tacgccgaga aggccgacgt ggcgctcagc gaggccgagg cgacggcgac aggtggtccc
       61 gaagtggagg cgatggccac cggtaccccg gtcgacggcg ccgccggcgt cgagctggcc
      121 gaggccggaa ggcagccagc cgtcgacgcg gctgccggac ttgagcccta cgccgagaag
      181 gccgacgtgg cgctggcaga cgcctcggag cagaagcccg agaccgctcc ctacgtggtc
      241 gagccgaccg aggagaagac tccggccgta gcgctcgact cgcccaagga gtatctcgtc
      301 gccatcgagc cgacaggccc gaagccgccc gttcagacgg cgccggccgt ggagacgaag
      361 ccggcgggta ccaaggtcgc tccagcggct cccgctccag cggcttccgc tccagcggcg
      421 acgatcgtct cgggcgcgct cgagaagggc cgctactaca tacagatagg cgcctacgcc
      481 agcgaggcgg ccgctaagga cgcggcctcg cgtctgagcg tcggctacat ggtggtcgtc
      541 cagcaggtga cgaccggagg caaggccacc tggaaggtct tcgtaggccc cctatcccga
      601 gacgagagcg gcgtggccct cgtgcgggtc aggtcgatgg gctaccgcga cgccttcgtg
      661 aagagcggcg gctgattgcg gtcagacgcg ggccttcgga tgacgatggc ccgcgtagac
      721 catgacggcc tgcgcggccg ctccggcgcc cagtaagagg gccaagccta tgaacactgg
      781 tggcatcgat agggcctggt agacgaagcc gccgctgacc gatccggtta tctggccgaa
      841 ggtcaggacc gattcgtgca cgctcgcccg cttgtcgcgg tcggacgccc cgctcacccc
      901 gtagaacagc gagttattgt acaccatggc ctggagtagc ccgatgaccg tgaaacctaa
      961 ggcgaagccc gcagcgttcc gggccgccgc caggagggcg accgtggcgg cggcggcgat
     1021 ggacagggcc gggatggcgg ctcgcttgaa ctgccagaac gtcagcctgc cgagcgccag
     1081 gaacccgacc gtcgtggccg cggctcgtat cgtcaacgct aagcctatcg tcgactcgct
     1141 taggccgagc tcgtcgctcg cgaataccgg gaatacgttg atcacgacgc ccatcaccgc
     1201 gtaggcgagg aatacgccga gccaagccgg atagcggagg ggggtcgagc ggtcgacgac
     1261 ggcttcaacc tcggcggcct cggccgatgg ctcctcgtcc aaacggacga ataacctcga
     1321 gacggt
//
LOCUS       DAOOLN010000706         1325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_134219_length_1325_cov_2.995276, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000706 DAOOLN010000000
VERSION     DAOOLN010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134219_length_1325_cov_2.995276"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="PLW80_08935"
     CDS             complement(<1..1222)
                     /locus_tag="PLW80_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPB66676.1"
                     /translation="MPTKQRLIRYLAALAIFAAADIWIFATLRSTLRDDAARSRDSSL
                     ALLAKSAPTDLDLIGDWLASVPGSLPGGAAIYLEPSEDDPYAYEPYAADPAALALWLE
                     LEPSDDAQKGLDSAYYFENYASPAKATLDDTTVRLFYAPITDETEGELLGVAVFMAPE
                     TAAPGIERLLTVFAIGAFALFAALTGVSRFARDPTVGYAVLTLAGLSVVFIVFPLVEA
                     FRLSFMKDGQASLAVWADALSGAHLQALWGSIRLGVWTASTSTLVGFLFAFVIHRTSA
                     RGKKFLAAVAQLPVISPPFSLTLSIILLAGNNGLITKQLLGLDNFSIYGLGGLVLVQT
                     IGMFPIAYLTLAGVLQGLDATLEEAALDMNASRWQVFRTVTLPLAVPGLLSAWLLVFT
                     NSLADFANPLLLAGN"
BASE COUNT          237 a          415 c          493 g          180 t
ORIGIN      
        1 agttgcccgc gagcaggagc gggttggcga agtcggccag cgagtttgtg aacacgagca
       61 gccaggcgga caggaggccg ggtaccgcga gcggaagggt gaccgtgcgg aagacctgcc
      121 aacggctcgc gttcatgtcg agcgcggctt cctcgagcgt cgcgtcgagg ccctgcagga
      181 cgccggccag cgtcaggtag gctatcggga acatgcctat cgtctgtacc agcacgaggc
      241 cgccgaggcc gtagatggag aaattgtcca ggcccaagag ttgcttggtg atgaggccgt
      301 tgttgccggc gagcaggatg atcgacaaag tcagcgagaa cggcggcgag atgaccggca
      361 gctgggcgac cgcggccagg aacttcttgc cgcgggcgct ggtgcggtgg atcacgaagg
      421 cgaacaggaa gccgaccagg gtggaggtgc tggccgtcca gacgccgagg cgtatcgagc
      481 cccagagcgc ctgcagatgg gcgccggaca gtgcgtcggc ccagaccgcc agcgatgcct
      541 ggccgtcctt catgaacgat agcctgaagg cctcgaccaa cgggaagacg atgaacacga
      601 cggacaggcc cgccagggtg agcaccgcgt agccgacggt cgggtcgcgg gcgaagcgcg
      661 agacgccggt cagcgcggcg aacagggcga aggcgcctat cgcgaacacg gtcagcaggc
      721 gctcgatgcc tggcgcggcg gtctccggcg ccatgaagac ggcgacgccg agcagctcgc
      781 cctccgtctc gtcggtgatt ggcgcgtaga agagccgcac cgtcgtatcg tcgagcgtcg
      841 ccttggccgg cgacgcgtag ttctcgaaat aataggccga gtcgaggccc ttctgggcgt
      901 cgtcgctcgg ctcgagctcg agccagaggg ccagggcggc ggggtcggcg gcgtagggct
      961 cgtacgcgta cgggtcgtcc tcggacggct cgaggtagat agccgcgccg cccggcagcg
     1021 accctggcac tgacgccagc cagtccccga tcaggtcgag atcggtcggc gcgctcttgg
     1081 cgagtagcgc gagcgacgag tcgcgcgatc gggcggcgtc atcgcgcagg gtcgagcgca
     1141 gcgtcgcgaa gatccagata tccgcggcgg cgaagatcgc cagggccgcc aggtatcgta
     1201 tcagtctctg tttggtaggc acggaacgct ccggttacta tacggaatag ggccgacctc
     1261 tcggccggcc cctgccgcag cacgcgccag ccgcccggcg gcgccctgcg cgccaaccac
     1321 agcgc
//
LOCUS       DAOOLN010000707         1323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_134531_length_1323_cov_2.791798, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000707 DAOOLN010000000
VERSION     DAOOLN010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134531_length_1323_cov_2.791798"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="PLW80_08940"
     CDS             <1..1321
                     /locus_tag="PLW80_08940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HPB66677.1"
                     /translation="AGAVEATASTGGQLMPPIMGAAAFLMSEMTGIPYVRIIGAAVIP
                     ALLYYFGVWAGVHFEAKKLGLRGMTKEELPNLKEIFFGRGYLMIPLVGIVWLLVTGYT
                     PMLAAFYAIVLSIAAAVLGWWAPLPIGGMLVVFAIMKPIFKVGPYGADGALRYLTQMT
                     PLGALAVLGAILLGFAILKKKPNIGFKEIVGGLESGARGAIGVLAATACAGIIIGVVT
                     KTGLGLKLGSVLVSLANGNLILTLFFTMITSIILGMGVPTTANYIITSTIAAPAIIML
                     LRMKFPELPIDSISIVLPAHMFAFYFGIIADVTPPVALAAFAGAGIAKANPMKTGLAA
                     SKLAIAAFLVPYIFVMNPMMLMIDVTFWSGLLMVATSMLGIIGVSSGIMGYLLCRSLW
                     WERILMAAGGVLMIDPGAVTVIIGIGLIVVGVVSQVIRKRVDSGKTA"
BASE COUNT          209 a          457 c          399 g          258 t
ORIGIN      
        1 cgccggagcc gtcgaggcta cggcgtcgac cggcggccag ctgatgccgc ctatcatggg
       61 cgccgcggcc ttcctgatgt cggagatgac cggtataccc tacgtgcgca tcataggcgc
      121 ggctgtgata ccggccctgc tctactactt cggcgtctgg gccggcgttc acttcgaggc
      181 caagaagctc ggcctgcgcg gcatgaccaa ggaagagcta ccgaacctca aggagatctt
      241 cttcgggcgc ggctacctga tgatcccgct cgtcggcatc gtctggctgc tcgtcaccgg
      301 ttataccccg atgctggcgg cgttctacgc catcgtgctg tcgatagcgg ccgccgtgct
      361 cggctggtgg gccccgctgc cgataggcgg catgctcgtc gtcttcgcga tcatgaaacc
      421 gatcttcaag gtcggcccct acggcgccga cggcgcgctc aggtacctga cccagatgac
      481 cccgctcggc gccctcgcgg tgctcggcgc catcctactc ggcttcgcga tcctcaagaa
      541 gaagccgaac atcggcttca aggagatcgt gggcggcctc gagtcgggcg ccaggggcgc
      601 catcggcgta ctcgccgcga cggcctgcgc cggtatcatc atcggcgtgg tcaccaagac
      661 cggcctcggc ctcaagctcg gctcggtgct cgtcagcctg gccaacggca acctgatcct
      721 gaccctgttc ttcacgatga tcaccagtat catcctgggc atgggcgtgc cgacgaccgc
      781 gaactacatc atcacctcga cgatagcggc cccggccatc atcatgctgc tcaggatgaa
      841 attccccgag ctgcccatcg actccatctc gatcgtgctg cccgcccaca tgttcgcgtt
      901 ctacttcggc atcatcgccg acgtgacgcc tccggtggcg ctcgcggcct tcgccggcgc
      961 gggcatcgcc aaggccaacc cgatgaagac cggcctcgcc gcgtccaagc tggccatcgc
     1021 ggccttcctc gtgccgtata tcttcgtcat gaacccgatg atgctgatga tagacgtgac
     1081 cttctggtcc ggcctcctga tggtcgcaac ctcgatgctc ggcatcatcg gcgtatcgtc
     1141 cggcatcatg ggctacctgc tctgccgctc gctgtggtgg gagcgcatcc tgatggccgc
     1201 cggcggcgtg ctgatgatcg atcccggtgc cgtgaccgtc atcatcggca tcggcctcat
     1261 agtagtgggc gtggtctccc aggtgatacg gaagcgcgtc gactccggta aaaccgcgta
     1321 aga
//
LOCUS       DAOOLN010000708         1323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_134541_length_1323_cov_2.567035, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000708 DAOOLN010000000
VERSION     DAOOLN010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134541_length_1323_cov_2.567035"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1323
                     /gene="gpmI"
                     /locus_tag="PLW80_08945"
     CDS             <1..>1323
                     /gene="gpmI"
                     /locus_tag="PLW80_08945"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPB66678.1"
                     /translation="PVVLVVMDGVGYGKYEEGDAVAAADMRHFRAMEASCPSARLKAH
                     GTAVGLPSDDDMGNSEVGHNAIGCSRVFAQGAKLVSASIATGAMFGGETWKRLVSNVR
                     AADGRTLHFLGLFSDGNVHSHIDHLKAMLERARADGVRRVRVHVLLDGRDVGEQSALE
                     YLEPFEAFLAGLSGGGFDYRVASGGGRQYITMDRYGADWSMVERGWKAHVLGQGARFK
                     SAKEAVLHYRAEKPSLIDQDMHEFVIEEGGEPIGAVQDGDSFVYFNFRGDRALEITAA
                     FEDDDFPHFDRIRRPKIAYAGMMEYDGDLHVPSAYLVSPPSIDRTMGEYLAGSGVRML
                     AISETQKYGHVTYFFNGNRQGKFDEALEDYVEVKSDLVPFEQRPWMKAAEITDRVIAA
                     IESGQYRFIRLNYPNGDMVGHTGVYQAVVCGLEAMDLQLGRLKAAVEKA"
BASE COUNT          227 a          464 c          423 g          209 t
ORIGIN      
        1 ccccgtcgtg ctcgtcgtga tggacggcgt cggctacggc aagtatgaag agggcgacgc
       61 cgtcgccgcg gccgacatgc gccatttcag ggcgatggaa gcctcctgcc cctccgcccg
      121 cctcaaggcc cacggtacgg cggtcggact gccgtccgac gacgacatgg gcaatagcga
      181 ggtcggccat aacgccatcg gctgcagccg cgtgttcgcc cagggcgcca agctcgtctc
      241 ggcctcgatc gcgaccggcg ccatgttcgg cggggagacc tggaagcgcc tcgtatcgaa
      301 cgtccgggcc gccgacggcc ggacgctcca tttcctcggc ctcttctccg acggcaatgt
      361 gcactcgcac atcgaccacc tgaaggcgat gctagagcgc gccagggcgg acggcgtcag
      421 gcgcgtccgc gtgcacgtcc tcctcgacgg tcgcgacgtc ggcgagcaga gcgcgctcga
      481 gtacctcgag cccttcgagg ccttcctcgc ggggctgtcg ggaggcggct tcgactaccg
      541 cgtcgcctcg ggcggcggca ggcagtacat cacgatggac cgttacggcg ccgactggtc
      601 catggtcgag cgcggctgga aggcccacgt cctcggccag ggagcccgct tcaagagcgc
      661 caaggaagcc gtcctccatt atcgcgccga gaagccgtcg ctgatcgacc aggacatgca
      721 tgagttcgtc atcgaggagg gcggcgagcc gatcggtgcc gttcaggacg gcgactcctt
      781 cgtctacttc aacttccgcg gcgaccgcgc gctcgagatc accgcggcct tcgaggacga
      841 cgactttccc cacttcgacc gaatcagacg ccccaagatt gcctacgcgg gcatgatgga
      901 gtacgatggc gacctgcacg tgccttcggc ctacctcgtc agccccccgt ccatcgaccg
      961 cacgatgggc gagtacctcg ccggatccgg tgtcaggatg ctcgcgattt ccgagaccca
     1021 gaaatacggc cacgttacct acttcttcaa cggcaaccgc cagggcaagt tcgacgaggc
     1081 gctcgaggac tacgtcgagg tcaagagcga cctcgtaccc ttcgagcagc gtccctggat
     1141 gaaggccgcc gagatcaccg accgcgtgat cgccgcgatc gagagcggcc agtaccgttt
     1201 catccgcctc aactatccca acggcgacat ggtcggccac accggcgtct accaggccgt
     1261 cgtctgcggc ctcgaggcca tggacctgca gctcggccgg ctcaaggccg cggtggagaa
     1321 ggc
//
LOCUS       DAOOLN010000709         1323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_134542_length_1323_cov_2.558360, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000709 DAOOLN010000000
VERSION     DAOOLN010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134542_length_1323_cov_2.558360"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..572)
                     /locus_tag="PLW80_08950"
     CDS             complement(24..572)
                     /locus_tag="PLW80_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPB66679.1"
                     /translation="MRITTKGRYALRAVVALARLSDGSSPVSIKTIGGEEGLSAEFLE
                     QIFFRLKKAGIIRSIRGPGGGFVFAKPLSAILLKDIVMASGEGVDLATCSGDGDEERC
                     DRVDRCAAGRVWGNCQTISTRTSRASAWSRSWHRTAGKPRPSPDSLLAVDERLEPLPL
                     RVVYQAHIAAERYEFPDVHERA"
     gene            937..>1323
                     /locus_tag="PLW80_08955"
     CDS             937..>1323
                     /locus_tag="PLW80_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPB66680.1"
                     /translation="MARFKVSYAPSVRRLPSYLLLAKQAQDHGDEYISGTMIAIELNL
                     EPIQVRKDLSITGIVGKPKRGYPVGPLIAAIESYLNWDKRVDAVVVGAGNLGSALTGF
                     PGFKQHGLHIVAGFDADPAKVGATIYD"
BASE COUNT          249 a          398 c          387 g          289 t
ORIGIN      
        1 acatggagtc gtgcggcgtc aggctacgcg cgttcatgca cgtcagggaa ctcatagcgc
       61 tctgccgcga tatgggcttg atagacgacg cggagcggga gcggctcgag gcgttcatcg
      121 acggcgagta ggctatccgg ggacggccta ggctttccgg ccgtcctgtg ccaggatctg
      181 ctccaggctg acgctcgtga ggtacgtgtc gatatagtct gacagttccc ccagaccctg
      241 ccggccgcgc agcgatcgac ccggtcgcaa cgctcctcgt cgccgtcgcc cgagcaggtg
      301 gcgaggtcga ccccctcgcc ggaagccatg acgatgtcct tgaggagtat agccgataac
      361 ggcttcgcga agacaaagcc tccgccggga ccccggatcg accggatgat gcccgccttc
      421 ttgagcctga agaagatctg ctcgaggaac tcggccgata aaccctcttc gccgccgatg
      481 gtcttgatcg agacggggga ggatccgtcg ctcaacctgg cgagggccac gaccgccctg
      541 agcgcgtagc ggcccttcgt cgtaatcctc atgcgttctc tcctcgaatc ggcgcgttta
      601 cgctcgggat gcgtccgggc ctgtgatcct actatgaata tgctcgatag agaagacata
      661 gcttcgcggc gggcgactcg atgggctcgt ccaccgatac cgcttgcata atcatagcat
      721 agcttgtata tttattctgt agttgctccg gcgctacaac cggccccgtg gtttacctcc
      781 tttttccacg gggccttcct atgccttgac gccggtcgtg tcgatagcga aacgctatat
      841 aaaaaacctt atattcttga ctttactaga cgcttctgac tattcttcat tccaatgtag
      901 tgctggtatc tatacttttc gcacgagagg tgttccatgg cgcgatttaa agtttcgtac
      961 gctccgtccg tgcgaaggct accctcgtat ctcttgctcg cgaagcaggc tcaggatcac
     1021 ggtgacgagt atatttccgg cacgatgata gcgatcgagc tgaatctcga gccgatccag
     1081 gttcgcaagg acctctcgat taccggcatc gtcggcaagc cgaaacgcgg ctatcccgtg
     1141 gggcccctga tcgcggcgat cgaatcgtac ctgaactggg acaagcgcgt cgacgcggtc
     1201 gtggtcggag cgggcaatct cggtagcgcc ctgaccggat tcccgggctt caagcagcac
     1261 gggctgcata tcgtggccgg cttcgacgcc gatcccgcga aagtgggtgc taccatctac
     1321 gat
//
LOCUS       DAOOLN010000710         1323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_134545_length_1323_cov_2.533123, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000710 DAOOLN010000000
VERSION     DAOOLN010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134545_length_1323_cov_2.533123"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PLW80_08960"
     CDS             complement(<1..706)
                     /locus_tag="PLW80_08960"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPB66681.1"
                     /translation="MLRSTRFDIDLDAIAHNLRQVKGMVGAPNDGPEGDDEAGRGAAS
                     TPAFTPVKIAAVVKADAYGLGAVRVARALLREGVDMLAVACLPEALKLRRAYPDAAIL
                     VMGHTPDEYLPVAARERVTCSIFDLGQAEALSAAARSAGVRAVAHVKVDTGMNRLGLK
                     PGLFEPGTGPGSAAELLCRMASLPSLELEGIFTHLALYDAASDAAQFAAFTRLVDEAA
                     SLGLRFALRHVCDSIGL"
     gene            complement(700..>1323)
                     /gene="alr"
                     /locus_tag="PLW80_08965"
     CDS             complement(700..>1323)
                     /gene="alr"
                     /locus_tag="PLW80_08965"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPB66682.1"
                     /translation="ESVFARLQLERFNEATAAIEAAGVRGFARHIANSSAYIRMPASR
                     LDMGRVGIAVYGLGVAGLEADGPALVPAMALKAKVTQVRTLPAGDSVGYGRTFVARRA
                     SRIGTLPLGYADGVSRALSNRGWVGLPSGKAPIVGTVCMDQFMVDLTDLPDEGEGATA
                     TLFGPGGPSAGELAELLGTIDYEIISSVSVRVPRIYLDSRLEEEEPC"
BASE COUNT          197 a          468 c          476 g          182 t
ORIGIN      
        1 tcaggccgat cgagtcgcag acgtgcctga gcgcgaagcg caggccgagc gaggccgcct
       61 cgtcgacgag ccgcgtgaac gccgcgaact gggccgcgtc ggaggccgcg tcgtagagcg
      121 ccaggtgggt gaatatcccc tcgagctcga gcgacggcag ggacgccatg cggcagagga
      181 gctcggccgc cgagcccggc ccggtccccg gctcgaacag cccgggttta aggccgaggc
      241 ggttcatgcc ggtatcgacc ttgacgtggg cgaccgccct gaccccggcc gatcgggccg
      301 cagccgacag ggcctcggcc tgcccgaggt cgaagatcga gcacgtgacg cgctcgcggg
      361 ccgcgacggg caggtactcg tcgggcgtgt ggcccatcac caggatcgcc gcgtccggat
      421 atgcccgcct gagcttgagc gcctcgggca ggcaggcgac cgcgagcatg tcgacgccct
      481 cgcgcaggag ggcgcgggct acgcgcaccg caccgaggcc gtacgcgtcg gccttgacga
      541 cggccgcgat ctttaccgga gtgaacgccg gagtggacgc ggcgcctcgt cccgcctcgt
      601 cgtcgccctc ggggccgtcg ttcggcgcac ctaccatgcc cttgacctgc ctgaggttgt
      661 gggcgatggc gtcgaggtcg atgtcgaagc gggtagacct cagcacggct cctcctcttc
      721 caggcggcta tccaggtaga tcctgggcac gcgcacggag accgagctga ttatctcgta
      781 gtcgatggtg ccgagcagct ccgccagctc cccggccgaa gggccgcccg gcccgaacag
      841 ggtcgcggtc gcgccctcgc cctcgtcggg caggtcggtc aggtcaacca tgaactggtc
      901 catgcagacc gtgccgacga tgggggcctt cccggacggc aagccgaccc agccgcggtt
      961 ggacagggcc cgcgacacgc cgtcggcgta gccgagcggt aacgtgccta tcctcgaggc
     1021 cctgcgggcg acgaaggtgc ggccgtagcc gacgctgtca cccgcgggca gcgtcctgac
     1081 ctgcgtgacc ttggctttga gcgccatcgc gggaaccagg gccggtccgt cggcctcgag
     1141 cccggcgacg ccgagcccgt agacggcgat gccgacccgt cccatatcca gccgagaggc
     1201 gggcatccgt atatacgcgg agctgttggc gatgtgccgc gcgaagccgc gcaccccggc
     1261 ggcctcgatg gcggccgtcg cctcgttaaa gcgctcgagc tggagccgcg cgaagacgct
     1321 ctc
//
LOCUS       DAOOLN010000711         1322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_134651_length_1322_cov_3.470403, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000711 DAOOLN010000000
VERSION     DAOOLN010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134651_length_1322_cov_3.470403"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PLW80_08970"
     CDS             complement(<1..555)
                     /locus_tag="PLW80_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPB66683.1"
                     /translation="MLPVMLIAAQLLVAQDRGLAVQARQVTGDPSFDIGRQYAVIIGI
                     DRYQEWNSLRNAVSEARNVKAILESRYYIDEFIELYDGAATAAGIRKLFAETLPRKIR
                     KEDSLFIFYAGHGYLDSSNTGFWIASDGVKDVFDQRGWIPNQQIRNYLSLLKAQRVLV
                     VADACFSGDFLNVSRGAGPTIDSAY"
     gene            complement(744..>1322)
                     /locus_tag="PLW80_08975"
     CDS             complement(744..>1322)
                     /locus_tag="PLW80_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPB66684.1"
                     /translation="RYRLRGILMKGNRMYVDIGSMLDEIFDAAKNFGDQMKDNFGPGG
                     PGCATGPGGQRSNPWFGMMDQDENSDWYPSFSYPPMNIFLTQDRSMVFEFALSGFDEN
                     DISLSFQGDYMVFSARIGLEAAPEEGVRYFKRRLKLKDIEKQKYYVPAEKFDQAMVKA
                     VFKNGILRVTVPPKESDEINEGIKIEIVKEGE"
BASE COUNT          248 a          383 c          420 g          271 t
ORIGIN      
        1 gtaggcgctg tctatcgtcg gcccggcccc gcgcgacacg ttgaggaagt cgcccgagaa
       61 gcacgcgtcg gcgacgacga ggacgcgctg ggccttgagc agggacaggt agttgcgtat
      121 ctgctggttc ggtatccagc cccgctgatc gaacacgtcc ttgacgccgt ccgaggctat
      181 ccagaagccg gtgttcgacg agtcgaggta gccgtgccct gcgtagaaga tgaacagcga
      241 gtcttccttc cggatcttgc gcggcagggt ttcggcgaac agcttgcgaa taccggccgc
      301 cgtcgcggcg ccgtcgtaca gctcgatgaa ctcgtcgatg tagtagcgcg actcgaggat
      361 ggccttgacg ttcctggcct cggagaccgc gttgcgtagc gagttccatt cctggtagcg
      421 gtcgatgccg atgatcacgg cgtactggcg gccgatgtcg aaggacgggt cgccggttac
      481 ctggcgtgcc tggacggcca ggccgcgatc ctgagcgacg agtagctggg cggcgatgag
      541 catgaccgga agcatgaccg ataggcggcg ggagactgac ggtccgatca aagcaggatt
      601 gcgcatggga acctccactt tcatcagagt catactatag cccctttata gactcctata
      661 aaggaaaatg taaaaaaaag cggccgggat gagtcccggc cgcctcgtcg atctctatcg
      721 ccggatacta ccggcccgag cgactactcg ccctctttca cgatctcgat cttgatgccc
      781 tcgtttatct cgtcggactc tttcgggggc acggtgacgc gtaggatgcc gttcttgaac
      841 acggccttga ccatggcctg gtcgaacttc tccgcgggca catagtactt ctgcttctcg
      901 atgtccttga gcttgagccg gcgcttgaag tagcgcacgc cctcctcggg ggcggcttcg
      961 agccctatgc gggcggagaa gaccatgtag tcgccctgga acgataggct gatgtcgttc
     1021 tcgtcgaagc cggacaacgc gaattcgaag accatcgaac gatcctgggt gaggaagatg
     1081 ttcatcggag ggtaggagaa gctcgggtac cagtcggagt tctcgtcctg atccatcatg
     1141 ccgaaccagg ggttggagcg ctggccgccg ggaccggtgg cgcatccggg ccctcccggg
     1201 ccgaagttgt ccttcatctg gtcgccgaaa ttcttggcgg cgtcgaagat ctcgtcgagc
     1261 atcgacccga tgtcgacgta catgcgattg cctttcataa gtatccccct aaggcggtat
     1321 cg
//
LOCUS       DAOOLN010000712         1322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_134712_length_1322_cov_2.407261, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000712 DAOOLN010000000
VERSION     DAOOLN010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134712_length_1322_cov_2.407261"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PLW80_08980"
     CDS             complement(<1..558)
                     /locus_tag="PLW80_08980"
                     /inference="COORDINATES: protein motif:HMM:NF016365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HPB66685.1"
                     /translation="MIATIVNALAIALGTAIGLLARKGFSERARDAAYNATGIISLVI
                     GIGMAVTTEHVLAFAIAVIAGGLIGTALDIEGAIYRLGEWLRSRFARRRAVGSRVIDG
                     SAEADAESRDGGFAYGFLNASVLFCVGAMALVGSFKAGAEGDYDLILTKSVMDGVIAV
                     MFAGAMGVGVGFSALSVLVYQGVLTL"
     gene            646..948
                     /locus_tag="PLW80_08985"
     CDS             646..948
                     /locus_tag="PLW80_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66686.1"
                     /translation="MAIRMEAEREGRIYCANCIHCKLVPSPAGGGQYYLRVRCDAGMW
                     RKKLGGEKLYKYCTVAYRSIEGCSRYEDMGDGPEFMRDLRASLPAADELYDAPPVR"
     gene            988..>1322
                     /locus_tag="PLW80_08990"
     CDS             988..>1322
                     /locus_tag="PLW80_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66687.1"
                     /translation="MVCSFTALDEYFGVSPAPPRFERFDGDLVGLARTADGLAFPGLP
                     YADAVGGSPSARVLYRCSDGEAGVAYPQAALARLPAKRSFDARGGVYESLADPPPRTG
                     PARAAPRPAP"
BASE COUNT          225 a          463 c          428 g          206 t
ORIGIN      
        1 cagggtcagg acgccctggt aaaccagcac cgagagggcg gaaaagccga cgccgacgcc
       61 catggcgccg gcgaacatga cggctatgac gccgtccatc acgctcttgg tcaggatcag
      121 gtcgtagtcg ccctcggccc cggccttgaa cgagccgacc aaggccatcg cgccgacgca
      181 gaacagcacc gaggcgttca ggaaaccgta ggcgaaaccg ccatcccggg actcggcgtc
      241 cgcctcggcg ctcccgtcga tgacgcggct accgacggcg cggcggcggg cgaagcgcga
      301 gcgcagccat tcacccaggc gatagattgc gccctcgatg tcaagcgccg tgccgatgag
      361 cccgccggct atgacggcta tcgcgaacgc tagcacatgc tcagtcgtca cggccatgcc
      421 gatgccgatc acgagcgaga taatgccggt agcgttatag gcggcgtcgc gcgcgcgctc
      481 ggagaagccc ttgcgcgcca ataggccgat ggccgtgccc agcgcgatgg ctagcgcatt
      541 tacgattgtc gcgatcatgg ttcgctccga tattgaagac gacgatggat ggcgcgggct
      601 cgcttgaccg ctcgatgatc attatatact atggtccgat gaaaggtggc aatcaggatg
      661 gaagctgaac gcgaaggccg tatctactgc gcgaactgca tccattgcaa gcttgtcccg
      721 tctccagccg gcggcggcca gtactacctg agggtgcgct gcgacgccgg catgtggcgc
      781 aagaagcttg gcggcgagaa gctctacaag tactgcaccg tcgcctaccg cagcatagag
      841 ggttgctccc gctacgagga catgggcgac ggccccgagt tcatgcgcga cctccgcgcg
      901 tcgctaccgg ccgccgacga gttgtacgac gcgcctccgg tccgctgacc ggccctttca
      961 tccaacttac atagcctgga ggcctcggtg gtttgctcgt tcacggcgct cgacgagtat
     1021 ttcggcgtca gccccgcccc tccgcgcttc gagcgcttcg acggcgatct ggtcggcctg
     1081 gccaggacgg cggacggcct ggccttcccc ggcctgccct acgccgacgc cgtaggcggc
     1141 tcgccgtccg cccgcgtgct ctaccgctgc tccgacggcg aagccggcgt cgcctatccc
     1201 caggccgcgc tcgcgaggct cccggccaag cgctccttcg acgcgcgcgg cggcgtgtac
     1261 gagagcctgg ccgacccgcc gcctcgaaca ggaccagctc gggcggcgcc gcgcccggcg
     1321 cc
//
LOCUS       DAOOLN010000713         1321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_134862_length_1321_cov_2.326224, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000713 DAOOLN010000000
VERSION     DAOOLN010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134862_length_1321_cov_2.326224"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="PLW80_08995"
     CDS             complement(<1..922)
                     /locus_tag="PLW80_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66688.1"
                     /translation="MNATIRAFAIIAAIACATPFRQASCDEYYSERGFSIDLPEGFVF
                     LGGDGSSRFTFGLPDGSVEIDLLAYPDDRFASARDGAEDTARRLSGRGAFASFDYQGR
                     GAAFGELTFGSGRSAMRGYGLFVNDVAPPADSAGAPYDLVVLAYAPADGYDEYADFVA
                     SAIDGFSIDYGRRAAPGPLGTAGRAALGAARPRPVTLRFGKAEIRADWNPAEAALSQQ
                     LVEREYRVLTAYGQSPELIDAAIARFYRMAFRDAAPSLDRLALELSAAWESGAWAGAR
                     PGPSLGAGATEPAMAGPRFGATTEPRGYAEA"
     gene            complement(1000..>1321)
                     /locus_tag="PLW80_09000"
     CDS             complement(1000..>1321)
                     /locus_tag="PLW80_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66689.1"
                     /translation="SVTARPTPLHQFERYRYRMFFFEPGVGRPVMSADLESDILGAWR
                     LTVTTASGSSIAMSFDEAPDYETFKAAALSYADAAAERARAGTAAGRSTKERATAKRG
                     PRRA"
BASE COUNT          203 a          448 c          487 g          183 t
ORIGIN      
        1 gggcctcggc gtagcctcgg ggctcggtgg tcgcgccgaa gcgcggcccg gccatcgcgg
       61 gttccgtcgc cccggcgccg agcgacgggc cgggcctcgc gccggcccag gcgcccgatt
      121 cccacgcggc cgacagttcc agggccaggc ggtcgagcga tggagccgcg tcgcggaacg
      181 ccatgcggta gaacctggct atcgccgcgt cgatcagctc gggcgactgg ccataggccg
      241 tcagcacgcg gtactcgcgc tctaccagtt gctgcgacag cgccgcctcg gccgggttcc
      301 aatcggcgcg gatctccgcc ttgccgaagc gcaaggttac cggcctcggc cgcgccgcgc
      361 ctaaggccgc gcggccggcg gtgccgagcg gcccgggggc cgccctgcgg ccgtagtcga
      421 tcgagaagcc gtcgatcgca gacgcgacga agtcggcgta ttcgtcatag ccgtcggccg
      481 gcgcataggc cagtacgacg aggtcgtagg gcgctccggc ggagtcagcc ggcggcgcca
      541 cgtcgttcac gaagagcccg tagccgcgca tggccgatcg tcccgagccg aaggtcagct
      601 cgccgaacgc cgctccgcgg ccctgatagt cgaaggaagc gaaggcgccg cgcccggaaa
      661 gtcgcctggc ggtatcctca gcgccatcgc gggcgctggc gaagcgatcg tcgggatagg
      721 ctagcaagtc tatctcgaca gacccgtccg gtagcccgaa ggtgaatcgg gacgatccgt
      781 cgccgcctag gaagacgaag ccctcgggca ggtcgatcga gaagccgcgc tcggagtagt
      841 actcgtcgca ggaggcctgc cggaagggcg tggcgcaggc gatggccgct atgatggcga
      901 aggcgcgtat cgtcgcattc atacgcagag tatagcgcat aaccggataa agggtgagcg
      961 gtcgcgacgc tgagcggtcg cgctccggcg tggccatgct caggcgcggc gaggaccccg
     1021 cttggccgta gcccgctcct tggtcgaccg cccggccgcg gttcccgccc gcgccctttc
     1081 ggccgcggcg tcggcgtagg acagggccgc cgccttgaag gtctcgtagt ccggggcctc
     1141 gtcgaacgac atggctatgc tcgatccgga cgcggtcgtc acggtcagcc tccaggcgcc
     1201 gaggatgtcg gattccaggt ccgccgacat gacggggcgc ccgacgccgg gttcgaagaa
     1261 gaacatgcgg tagcggtagc gctcgaactg gtgcaggggg gtcggcctgg ccgtcacgga
     1321 a
//
LOCUS       DAOOLN010000714         1319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_135195_length_1319_cov_2.360759, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000714 DAOOLN010000000
VERSION     DAOOLN010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135195_length_1319_cov_2.360759"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PLW80_09005"
     CDS             <1..521
                     /locus_tag="PLW80_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HPB66690.1"
                     /translation="SMSEAIREYPEIVQKYLEWSQDYKEDRVTILYDTMWQGTRVMAD
                     ALAKAIAEASPSTTVVSHCLSKFDKNDAITDVFRSKAVLVGSPTVGKGIMTAVAAALE
                     EIKGLRFKGKKAGAFGAYGWSGESVKVISDLLKASGFDVVDDGIKALWNPDDAAIAAT
                     AAYGKAFAAKLG"
     gene            535..870
                     /locus_tag="PLW80_09010"
     CDS             535..870
                     /locus_tag="PLW80_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPB66691.1"
                     /translation="MLEKEYRFTETDAFTMEKIIDDERVNINHIVVGPGEAVPVHVSN
                     SYVHQIVTRGTLSLSLEDGPEESHPASSIVAVPYNLKMAIRNRGDETLEFFVVKAPNP
                     RDMPPTKAI"
     gene            918..>1319
                     /locus_tag="PLW80_09015"
     CDS             918..>1319
                     /locus_tag="PLW80_09015"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPB66692.1"
                     /translation="MITNTNRGAGLRPVATPRPIADARTALAAVIAVALLSACATGRT
                     DGSGAKETVSSAPAVRPAEYGPRPLRVGHRGAAGLAPQNTMASFRVAIATGVDAIELD
                     LHLSSDGRLVVIHDPVVDKLTDGKGRVSAMSL"
BASE COUNT          248 a          448 c          423 g          200 t
ORIGIN      
        1 gctcgatgag cgaggcgatc cgcgaatatc cggagatcgt gcagaagtac ctcgagtggt
       61 cgcaggatta taaggaggac agggtcacca tcctctacga cacgatgtgg caaggcaccc
      121 gcgtaatggc cgacgcgctg gccaaggcca tcgccgaggc ctcgccgtcc acgacggtgg
      181 tgtcgcactg cctgtccaaa ttcgacaaga acgacgcgat caccgacgtg ttccgctcca
      241 aggccgtgct cgtcggctcg cccaccgtag gcaagggcat catgaccgcc gtcgccgcgg
      301 cgctcgagga gatcaagggc ctgcgcttca agggcaagaa ggccggggcc ttcggcgcct
      361 acggctggtc tggcgagtcg gtcaaggtaa tctccgacct gctcaaggcc tccggcttcg
      421 acgtggtcga cgacggcatc aaggcgctat ggaacccgga cgacgcggct atagccgcca
      481 cggcggccta cggcaaggct ttcgccgcta agctgggctg atcggagtag aggaatgctg
      541 gagaaagaat accgttttac cgaaacagac gccttcacga tggagaagat aatcgacgac
      601 gagcgcgtca acatcaacca catcgtcgtt gggcctggcg aggcggtgcc ggtgcacgtc
      661 tcgaactcgt acgtgcacca gatcgtgacg cgcggaacct tgagcctgtc cctcgaggac
      721 ggccccgagg agagccatcc ggctagcagc atcgtggcgg taccgtataa tctcaagatg
      781 gccatacgga accgcggcga cgagaccctc gagttcttcg tcgtcaaggc gccgaacccg
      841 cgcgacatgc cgccgaccaa ggcgatctag ggtatcggcg tgcggcggcc gcggcgcttc
      901 ggtgctatac tgctcccatg atcacgaata cgaaccgcgg cgccgggctc cgccccgtcg
      961 cgacgccccg ccctatcgcc gacgcccgga cggcgctggc cgccgtcata gccgtagccc
     1021 tgttatctgc ctgcgccacg ggccggaccg acggctccgg agcgaaagag accgtctctt
     1081 cggccccggc ggtcaggccc gccgagtacg gcccgcgccc cctccgcgtc ggtcaccgag
     1141 gcgccgccgg actcgcgccg cagaacacga tggcctcgtt ccgcgtcgcg atcgcgacag
     1201 gcgtcgacgc catagagctg gacttgcacc tgagctccga cggccgcctc gtcgtcatcc
     1261 acgacccggt cgtcgacaag ctgaccgacg gcaagggccg cgtatcggcg atgagcctc
//
LOCUS       DAOOLN010000715         1316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_135737_length_1316_cov_2.003965, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000715 DAOOLN010000000
VERSION     DAOOLN010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135737_length_1316_cov_2.003965"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..430)
                     /locus_tag="PLW80_09020"
     CDS             complement(65..430)
                     /locus_tag="PLW80_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66693.1"
                     /translation="MTIPGVTKLMHAQCDRAMLEGEFGPDITGSAESVAVVLTQGWCP
                     QWQAMARWLGEAAAEAGALAYYVEYDEEPFFERFMAWKEDELGNRSVPYLRYYRGGVL
                     VAESNYVSRDGVLARLRRG"
     gene            complement(442..669)
                     /gene="thiS"
                     /locus_tag="PLW80_09025"
     CDS             complement(442..669)
                     /gene="thiS"
                     /locus_tag="PLW80_09025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HPB66694.1"
                     /translation="MLWYILRSMTITLNGNAERFDSGPLTIRGLLLAKRWSFPLIVVS
                     INGKAVPRDDWDATTVADGDAVEATHLMSGG"
     gene            748..>1316
                     /locus_tag="PLW80_09030"
     CDS             748..>1316
                     /locus_tag="PLW80_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66695.1"
                     /translation="MPGDDRDNSLFATFRRSKGARLKRVIILPFFIVTALGFALSWVM
                     YVTGSRDAVSDAIRTIAVESSKRVSDQVYARLDQAVHVAAANAAFLGAYPRIDDEATA
                     IRLAFVEQLRHQPVLAIAAIGLESGDYLEAQRIGPGSYRVGGAGSSTGGALVFRPVLE
                     GGGFGERSVEASGYDPRDRPWYGAAIEADG"
BASE COUNT          222 a          454 c          417 g          223 t
ORIGIN      
        1 tgacggagaa agccctgggc gactaggcgg agcgacctag ggcgacgata ggcccgcggc
       61 ggcctcagcc gcggcgcagc ctagccagca cgccgtcccg cgacacgtaa ttgctctccg
      121 cgacaaggac gccgccgcgg tagtagcgaa ggtagggcac gctacggttc ccgagttcgt
      181 cctccttcca cgccatgaag cgctcgaaga acggctcctc gtcgtactcg acgtaatagg
      241 cgagcgctcc ggcctcggcc gccgcctcgc cgagccagcg ggccatcgcc tgccactgcg
      301 ggcaccagcc ctgggtcagg accacggcta ccgattcagc ggagccggta atatcggggc
      361 cgaactcgcc ttcgagcatc gcccggtcgc attgggcgtg cataagcttc gtaacaccgg
      421 ggatcgtcac accgtcactc ctcagccgcc cgacatcagg tgggtcgcct cgaccgcgtc
      481 gccgtccgcc accgtcgtcg cgtcccagtc gtcccggggc accgccttac cgttgatcga
      541 gacgacgatc agcggaaacg accagcgctt ggcgaggagc aggccgcgga tcgtgagcgg
      601 gcccgagtcg aaccgttcgg cgttgccgtt caaggtaatc gtcatgctcc tgagtatata
      661 ccataacatg acggcgattc tctatcgatt ctccatacag gtattttcct ttactgaaac
      721 gctaacgtcg gaatatactg acgcaccatg ccaggcgacg atagggacaa ttctctgttc
      781 gcgacgttca ggcgctcgaa gggcgccagg ctcaagcgcg tcattattct cccgttcttc
      841 atcgtcaccg cgctcggttt cgcgctgtcg tgggtcatgt acgtcaccgg ctcccgcgac
      901 gccgtgagcg acgccatccg gacgatcgcc gtcgagtcga gcaagcgggt ctccgaccag
      961 gtatacgcca ggctggacca ggccgtccac gtcgccgcgg ccaacgcggc tttcctcggc
     1021 gcctatccgc gcatcgacga cgaggcgacc gcgattcggc tcgccttcgt cgagcagctg
     1081 cgccatcagc cggtattggc catagcggcg ataggcctgg agagcggcga ctacctcgag
     1141 gctcagcgca tcggacccgg ctcgtaccgc gtcggcggcg ccggctcgag taccggcggc
     1201 gctctcgtct tcaggccggt gctcgagggc ggcggcttcg gagagcgatc cgtcgaggct
     1261 tcgggctacg acccgcgcga tcgcccctgg tacggcgcgg cgatcgaggc cgacgg
//
LOCUS       DAOOLN010000716         1314 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_135965_length_1314_cov_3.652105, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000716 DAOOLN010000000
VERSION     DAOOLN010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135965_length_1314_cov_3.652105"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1314
                     /locus_tag="PLW80_09035"
     CDS             <1..>1314
                     /locus_tag="PLW80_09035"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB66696.1"
                     /translation="GITEPEALDPDDPTAAAELIRMKRKFSFGAFRSVDELLDVRVID
                     GPVVGLRGGAFLRRLGVNRYEFICGGSAVDVDLNLGPGASYGAPYELPRSRISHEAFA
                     VVHLGEGDGWDPERPCMCSLVVHAGRRYLIDAGPNIEESLDAVGLGVGDLAGVFHTHV
                     HDDHFVGLTSLMRADKRLSYYAVPMVRRSATEKLRALCGLGADEFARLFDVVDLRPDE
                     WNDVGGFDVKPVYSPHPLETTILRFRVAVADGYRTYAHYADLSSFAVIDSMVTDDASV
                     AGITAAEASRAKAAYLEPADVKKVDVGGGMIHRDPDDFAGDRSGDLLLSHGDSAEALS
                     GKGFGRIASFGEVSLLAPERRDWAAEAAADALRGYFPDAPAEAIEALASGPRRERKAG
                     DRLLAAGNEPPAALLTLDGRVTRVDGVGPAASYGPGALLGAAAVL"
BASE COUNT          184 a          456 c          468 g          206 t
ORIGIN      
        1 acggcatcac cgagcccgag gcgctcgatc ccgacgaccc gacggcggcg gccgagctga
       61 tacgcatgaa gcgcaaattc tcgttcggcg cgttccgttc tgtcgacgag ctgctcgacg
      121 tccgcgtcat cgacggcccc gtcgtggggt tgaggggcgg cgccttcctg aggcggctcg
      181 gcgttaatcg ctacgaattc atctgcggcg gttcggcggt cgacgtcgac ctgaacctcg
      241 ggccgggagc gtcgtacgga gcgccgtacg agctgccccg atcgcggata agccacgaag
      301 cgttcgccgt ggtgcatctc ggcgagggcg acggctggga cccggagcgt ccgtgcatgt
      361 gcagcctcgt cgtccacgct gggcgccgct accttatcga tgctggcccg aacatcgagg
      421 agagcctcga cgcggtcggc ctcggcgtcg gcgacctcgc tggcgtcttc catacgcacg
      481 tccacgatga ccacttcgtc ggcttgacca gcctgatgcg ggccgacaaa cggctgtcgt
      541 actacgccgt gccgatggtc agacgctcgg ctaccgagaa gcttcgggcc ctctgcggcc
      601 tcggcgccga cgagttcgcc cgcctcttcg acgtggtcga cctgcggccg gacgagtgga
      661 acgacgtcgg cggcttcgat gtcaagccgg tctactcgcc gcacccgctc gagacgacta
      721 tcctccggtt cagggtcgcc gtcgcggacg ggtataggac ctacgcccat tacgccgacc
      781 tgagctcgtt cgcggtgatc gactcgatgg tcacggacga cgcctcggtc gccgggatca
      841 cggccgccga ggcgtcgcgc gccaaggcgg cctacctcga gccggcggac gtcaagaagg
      901 tggacgtcgg cggcggcatg atacaccgcg acccggatga tttcgccggc gatcgctcgg
      961 gcgacctgct cctgagccac ggtgactcgg cggaagcgct gtcaggcaag ggcttcggga
     1021 ggattgcctc gttcggcgag gtcagcctac tggcgcccga gcggcgggac tgggccgccg
     1081 aggcggccgc ggacgcgctc cggggctact ttcccgacgc ccccgccgag gcgatcgagg
     1141 ccctggcctc gggcccaagg cgcgagcgca aggcgggcga tcgcctgctc gccgccggaa
     1201 acgagcctcc ggccgcgctg ctgacccttg acggccgggt gacgcgtgtc gacggcgtcg
     1261 ggccagcggc gtcatacggg ccgggcgcct tgctcggggc ggccgccgtg ctgc
//
LOCUS       DAOOLN010000717         1313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_136156_length_1313_cov_2.639905, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000717 DAOOLN010000000
VERSION     DAOOLN010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136156_length_1313_cov_2.639905"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /locus_tag="PLW80_09040"
     CDS             complement(<1..1249)
                     /locus_tag="PLW80_09040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPB66697.1"
                     /translation="MILSQSLFAAAAFGLIAQDNGLSYEFLSLTNAAMLAAVLCIGHG
                     LTRFGLMSRFSPWAYAIVPLGVGGWFALGPFGVSARIVLFASAFTVVGALFAADVRRS
                     SKDEFGSLRALFYLFFIIVSVSSAGRGIRTVVWEPPRTIALDGSIGAASHLSALLIGF
                     MNMFGYFLLSSAHAERQLGLREREIRKRNEELLETIETKDALISVIAHDLRAPVWSAT
                     RYVRSHLVDFDGDLNVKRDSIVTLAEGLDRISGLLDSLLEWAMCASGRIKLQPVPVSL
                     GEVLGEAIADLGPTAELKGVSIEAGLSDGLVVADRRALATALRNLLSNAIKYSRQGGA
                     IKATIGPSRQAADSAAAAFGHKASLSVVIEDSGVGMTPDQVAKLFVPGRTKLTLGTSG
                     EQGKGFGLAISKRFVEAMGGSLLV"
BASE COUNT          275 a          417 c          410 g          211 t
ORIGIN      
        1 cgacgagcag gctaccgccc atcgcttcca cgaagcgttt actgatggct agcccgaagc
       61 ccttgccctg ctcgccggag gtaccaaggg tcagcttggt gcggccgggc acgaagagct
      121 tggcgacctg gtccggggtc atgccgacgc cggaatcctc gatgaccacc gagaggctcg
      181 ccttatggcc gaaggcggcg gccgcggagt cggccgcctg tctagacggc ccgatagtcg
      241 ccttaatagc tccgccctgc ctcgaatact tgatcgcgtt cgataatagg ttccgaagcg
      301 cggtcgcgag cgcccgccgg tcggccacga ccaagccatc ggataggccg gcctctatgg
      361 acacgccctt gagctcggca gtcgggccta gatccgcaat cgcctcgccg agcacctccc
      421 cgaggctgac cgggaccggc tgaagcttga tgcgcccgga agcgcacatg gcccactcga
      481 gcagcgagtc caggaggccg gaaatgcggt ccaggccctc ggccaacgtg acgatgctgt
      541 cgcgcttaac gttgagatca ccgtcgaagt cgacgagatg gctccgcacg taacgagtgg
      601 cgctccagac cggagcgcgc aggtcgtggg ctatgacgct gatcagcgcg tctttcgtct
      661 cgatggtctc gagcagctcc tcgttacgct tccgtatctc ccgctcccgt aaccccagct
      721 gacgctcggc gtgggcggac gacaggagga agtacccgaa catattcatg aagcctatca
      781 gcaacgccga caggtgcgag gccgcgccta tcgatccatc gagcgcgatc gtgcgcggcg
      841 gttcccagac gacagtccga atgccgcgac ccgcgctcga aacgctgacg atgataaaga
      901 ataggtagaa caacgcccga aggctaccga attcgtcctt actggaccgt cgaacgtccg
      961 ccgcgaagag cgcgccgacg accgtgaagg ccgaggcgaa taggacgatg cgggcgctga
     1021 cgccgaacgg tccaagcgcg aaccatccgc cgacgccaag cggcactatc gcgtaggccc
     1081 atggcgagaa tcgggacatg aggccgaagc gcgtcaagcc gtgcccgatg cacagcacgg
     1141 cggcgagcat ggccgcattg gtaagcgaca ggaactcgta cgacaggccg ttatcctggg
     1201 ctatcaaccc aaaggcggcg gccgcgaaca gggactgact gagtatcatc agcttatctg
     1261 tatgtgctat aatctaatct gacacttaga gagttggagt gaagatcaat gac
//
LOCUS       DAOOLN010000718         1313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_136159_length_1313_cov_2.528617, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000718 DAOOLN010000000
VERSION     DAOOLN010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136159_length_1313_cov_2.528617"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="PLW80_09045"
     CDS             complement(<1..854)
                     /locus_tag="PLW80_09045"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPB66698.1"
                     /translation="MRARLSKAVAPILIVAAFASLFAFSLAALAGMDREYREMQLGME
                     RSLRAVLRAVPAGRSLPPELEGLFAAVERLERIAVSVDLEEPRRALLRLVELYEPGLT
                     DTEAFLDRFCESYAILETDLARKRAALADSFYALIAALAIGFVVAAAIGATLSLRLQV
                     SRLEAVWSRRSLLASLRAEEGLRKRIARDLHDDVAQDLAAARMLCERAASPSDASDGQ
                     LTRLLSESISLLTSAGKRLRGLALDLRPPELERSGLAAAIESLCSRSFGQHLGLATFR
                     AEAGAAEAA"
BASE COUNT          239 a          453 c          438 g          183 t
ORIGIN      
        1 gcggcctcgg cggcgccggc ctccgcgcgg aacgtagcca gcccaagatg ctggccgaag
       61 ctcctcgagc acagcgactc gatggctgcc gcgagcccgg aacgctccag ctccggcggc
      121 ctcaggtcca gggccaggcc gcgcaggcgc ttacccgccg aagtcaggag gctgatggac
      181 tcggagagta atcgggtcag ctggccgtcg gaggcatcgg acggggaagc ggcccgctcg
      241 catagcatcc tggcggcggc caggtcctgg gccacgtcat cgtggaggtc gcgggcgata
      301 cgctttcgta gcccttcctc ggcccgtagc gacgcgagca ggctcctgcg cgaccagacg
      361 gcctcgagcc ggctgacctg caggcgcagg ctcagggtgg cgccgatagc ggccgccacg
      421 acgaagccga tggctagggc ggcgatgagc gcgtagaacg aatcggctag cgcggcccgt
      481 ttcctggcca ggtccgtctc caggatggcg tacgactcgc agaagcggtc caggaacgcc
      541 tcggtatcgg tcagacctgg ctcgtacagc tcgacgagcc ggagcagggc gcgccgcggc
      601 tcttccaggt cgacgctgac ggctatgcgc tccagccgct cgaccgcggc gaacagccct
      661 tcgagttccg gcggcaggga gcggccggcg ggtacggcgc gcagcaccgc ccggagcgag
      721 cgctccatgc ccagctgcat ctcccggtat tcccggtcca tgccggccag agccgcgagc
      781 gagaaggcga acagcgacgc gaaggccgcc acgatgagga tgggagctac cgccttagaa
      841 agcctggccc gcacgaacgc tccttgccgc cacgggctca tggatccgag gcgcgagagt
      901 cgagtataga ccagggccgg gaagcgggca atcgaatcgt tacgccagga tgaggaggcg
      961 agtcgtcgcg cgacggctcg ccggcgcgcc gccaggcgcg ctttcgtagc gctctatcgt
     1021 cgaggggcgc ggagcgcccc tcttttacgc tttttatatc cattaagccc ggggatcgcg
     1081 tatgcatccc ttgcgcaagt tacacaaaaa acctactata tagctgccca tagcaacgcg
     1141 cgccgtactc acgttcagtc ccgccggaag accgcgccac gcccgggcaa ctacacccca
     1201 aaggaacccg tatggctact aagaagaaca tggtgatgat cgacggcaac accgccgccg
     1261 cgcacgtcgc ccacgcctgc tccgaggtga tcgccatcta cccgatcacc ccc
//
LOCUS       DAOOLN010000719         1312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_136361_length_1312_cov_2.273667, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000719 DAOOLN010000000
VERSION     DAOOLN010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136361_length_1312_cov_2.273667"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLW80_09050"
     CDS             <1..374
                     /locus_tag="PLW80_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPB66699.1"
                     /translation="GQIGRDVGRVGDVLSAAVGPFDLIVANPPYVTTDLTDCLLASGD
                     GEPRMALDGGELGLDIYPALAAQAYALLADGGSLAVEIGEEQGRAVGAILEAAGFGEV
                     RVHVDLAGSDRVVSGGRHAVR"
     gene            361..>1312
                     /locus_tag="PLW80_09055"
     CDS             361..>1312
                     /locus_tag="PLW80_09055"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB66700.1"
                     /translation="MQYDERLDDYLGNFTDDEAQRIRSARAWAAELHSGSTRASGEPT
                     VTHPERVAAILAGMGMDADRVVAGLLHQAIEDSLATGAQVGERYGERVGALVDELSRM
                     ATLKAKNKTIQAAETIRKMLFAMTRDLRVIIVKLADKLDNMRTLKWLPEADRRRIAAE
                     CVDVFAPLADRLGISWMKDELEDLALKEINREAFDQIKLLVAAKKPEREAFLERARAE
                     IDAAARADGLNVEVMSRAKHFYSIYQKMRRRAKGADELYDLSGIRVLCDEESQCYAVL
                     GIVHGLWKPLDGRFKDYIAMPKSNGYRSLHTTVMSYDGTLL"
BASE COUNT          236 a          427 c          440 g          209 t
ORIGIN      
        1 tcggtcaaat cggtcgtgac gtagggcggg ttggcgacgt gctgtctgcc gccgtcggcc
       61 ccttcgacct gatcgtcgcc aacccgccct acgtcacgac cgatttgacc gattgcctgc
      121 tcgcctccgg cgacggcgag ccgaggatgg ccctggacgg cggcgagctc gggctcgata
      181 tctatccggc gctcgccgcc caggcttacg cgctcctcgc tgacggcggc tccctggccg
      241 tcgagatagg cgaggagcag gggagggcgg tcggcgccat actcgaagcg gccgggttcg
      301 gcgaggtgcg ggttcacgtc gacctggccg gctccgaccg cgtggtctcc gggggcaggc
      361 atgcagtacg atgaacggct cgacgactac ctaggtaatt ttacggacga cgaggcgcaa
      421 cgcatacggt ccgcgcgcgc ctgggcggcc gagttgcatt cgggcagcac gcgggcgtcg
      481 ggcgaaccga ccgtgactca cccggaacgg gtcgccgcca tcctggccgg catgggcatg
      541 gacgctgaca gggtcgtcgc cggactcctc catcaggcca tcgaggactc cctggccacg
      601 ggcgcgcagg taggagagcg ttacggcgag cgggtcggcg ctctcgtcga cgagctgtcg
      661 cgcatggcta ccctcaaggc caagaacaag accatccagg cggccgagac catacgcaag
      721 atgctgttcg cgatgacccg tgacctgcgc gtcatcatcg tcaagctggc cgacaagctc
      781 gataacatgc gtaccctcaa atggctgccc gaggccgacc ggcgccgcat cgctgccgag
      841 tgcgtcgacg tcttcgcgcc gctggccgac cggttgggta tcagctggat gaaggacgag
      901 ctcgaggacc tcgcgctcaa ggagatcaac cgcgaggcct tcgaccagat caagctgctc
      961 gtcgccgcca agaagcccga gcgcgaagcc ttcctggaac gggcgcgcgc cgagatagac
     1021 gcggcggctc gcgcggatgg actaaacgtc gaggtgatgt ccagggccaa acacttctat
     1081 tcgatatacc agaagatgcg caggcgcgcc aaaggcgcgg acgagctgta cgacctgtcg
     1141 ggcatccgcg tgctatgcga cgaggagagc caatgctacg ccgtcctcgg catcgtgcat
     1201 gggctctgga agcctctcga tgggcgcttc aaggactaca tagcgatgcc caagtccaat
     1261 ggttaccgta gcctgcacac cacggtgatg tcctacgacg gtaccctgct cg
//
LOCUS       DAOOLN010000720         1311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_136546_length_1311_cov_2.090764, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000720 DAOOLN010000000
VERSION     DAOOLN010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136546_length_1311_cov_2.090764"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="PLW80_09060"
     CDS             <1..868
                     /locus_tag="PLW80_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPB66701.1"
                     /translation="EEKAIPLDEKIVCHGEISYGDRVFRCHIRKPGHGPLDLAGALAE
                     SCDVYFWEVGRDRLGIERVVSYAMEFGFGQATGIDLPGEVGGFVPTPQWKERRFHEKW
                     LGGDTLNLAIGQGFMQTTPLQLADMMAMVVNDGVIYRPHLLKEVRDPRDGSIVTRVVP
                     EVLTASSISKETFKTTKEYLRGVITEGTARYPVNTKAVTVAGKTGTAEVGLADRWHSW
                     FVGYGPVDAADPDDVIVVVAMIEAANPWEWWAPYATNLVFQGYFAGQTYEEAAAQLGL
                     LGRSMPIGQRME"
     gene            868..>1311
                     /locus_tag="PLW80_09065"
     CDS             868..>1311
                     /locus_tag="PLW80_09065"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPB66702.1"
                     /translation="MASIRKLLSSMDWTIPIAIVILMVIGVLFIYSSGVTSAGDLVST
                     EYAKQIVWAATGLALLVACSLVDLKRVSDYIPIVYGLSLALLAYTLAFGKVVNGARSW
                     LGIIGDYGIQPSEFVKITSALFLARYLDRSVHEASTIKRFALSFAI"
BASE COUNT          240 a          420 c          412 g          239 t
ORIGIN      
        1 cgaggagaag gccatcccgc tcgacgagaa gatcgtctgc cacggcgaga tcagctacgg
       61 cgaccgcgtc ttccgctgcc atatacgcaa gccgggccac ggcccgctcg acctggccgg
      121 ggccctggcc gagtcgtgcg acgtgtactt ctgggaggtg gggcgcgacc gcctcggcat
      181 cgaacgcgtc gtatcctacg cgatggaatt cggcttcggg caggcgaccg gcatagacct
      241 tcccggcgag gttgggggct tcgtaccgac cccgcaatgg aaggagcgcc gcttccatga
      301 gaagtggctc ggcggcgaca cgctgaacct ggccatcgga cagggcttca tgcagacgac
      361 gccgctccag ctggccgaca tgatggccat ggtggtcaat gacggcgtca tctacaggcc
      421 gcacctcctc aaagaggtgc gcgacccgcg cgacgggtcc atcgttaccc gcgtcgtgcc
      481 cgaggtgctt accgcttcga gcatttccaa ggagaccttc aagacaacga aggaatactt
      541 gcgcggcgtc ataaccgagg gcacggcacg ctatccggtc aacaccaagg cggtcacggt
      601 agccggtaag acggggacgg ccgaggtggg tctggccgac cgatggcatt cctggttcgt
      661 cgggtacgga ccggtcgacg ccgccgatcc cgacgacgtc atcgtcgtcg tcgccatgat
      721 agaggcggcc aacccctggg aatggtgggc gccctacgcg acgaacctcg tattccaggg
      781 ctatttcgcc gggcagacct acgaagaggc agcggcccag ctaggcctgc tcggccgctc
      841 gatgccgata gggcagagga tggagtgatg gcgtcgatca ggaagctgtt atcgtccatg
      901 gactggacca taccgatagc tattgtgata ctgatggtca tcggcgtact gttcatctat
      961 tcgtccggag taacgagcgc cggagacctc gtctcgaccg agtacgcaaa gcagattgtg
     1021 tgggccgcca cgggcctggc cttgctggtc gcgtgctcgc tcgttgacct caagcgcgtc
     1081 tccgactata taccgatcgt ctacgggctc tccctggccc tgctcgccta taccctggcc
     1141 ttcggcaagg tcgtgaatgg cgcgcgctcc tggctaggca tcataggcga ctacggcatc
     1201 caaccctccg agttcgtcaa gataacctcg gccctgttcc tggctcgcta cctcgaccgt
     1261 agcgtccacg aagcgtcgac gatcaagcgc ttcgccctat cgttcgccat c
//
LOCUS       DAOOLN010000721         1310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_136617_length_1310_cov_3.442231, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000721 DAOOLN010000000
VERSION     DAOOLN010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136617_length_1310_cov_3.442231"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1310
                     /locus_tag="PLW80_09070"
     CDS             <1..>1310
                     /locus_tag="PLW80_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB66703.1"
                     /translation="QKFLGELNGHVEEMYSGHQVVKAFGYEKRSVARFEEVNDRLFDV
                     AWKAQFVSGIIMPTMNFINNIGYVLIAAIGGVMAAARAISLGDVQAFIQYAKQFTQPI
                     IQTANIANILQSTMAAGERVFELLDEAEESPDVPADSPAALAFAAASGQVSFEAVSFG
                     YSDDTPLMDGLSLEAPRGEAVAIVGPTGAGKTTLVNLLMRFYELKGGRIAVDGVDIRD
                     VPRGRLRRAFGMVLQDAWLFGGTIRDNIAYGREGATEDEIVAAAVAAHADHFIRVLPD
                     GYDTVLTEDATNLSQGQRQLLTIARAFLADPRILILDEATSSVDTRTELVVRKAMERL
                     MRGRTSFVIAHRLSTIRDAHTILVMNEGRIVEKGSHVELLAADGFYADLYRSQFLGDI
                     PAVAAMMPEAHFGRFTARKLYIHNCGHALLAYAGYLYGHEYGWQ"
BASE COUNT          235 a          443 c          423 g          209 t
ORIGIN      
        1 agcagaaatt cctgggcgag ctgaacggcc acgtcgagga aatgtactcg ggccaccagg
       61 tcgtcaaggc cttcggctac gagaagcgct cggtagcgcg cttcgaggaa gtgaacgaca
      121 ggctgttcga cgtggcgtgg aaggcgcagt tcgtctcggg catcatcatg ccgacgatga
      181 acttcatcaa taacatcggc tacgtgctga tcgccgcgat aggaggcgtg atggccgccg
      241 ccagggcgat cagcctgggc gacgtgcagg ccttcatcca gtacgccaag cagttcaccc
      301 agcccatcat ccagacggcg aacatagcca acatactgca gtcgacgatg gcggccggcg
      361 agcgcgtctt cgagctgctc gacgaggcgg aggagtctcc cgacgtcccg gccgactcgc
      421 cggccgcgct cgccttcgcg gccgcctccg gccaggtctc gttcgaggcc gtttcgttcg
      481 gctatagcga cgatacgccg ctcatggacg gcctgtcgct cgaggcgccg cggggcgagg
      541 ccgtagcgat cgtcgggccg accggcgccg gcaagacgac cctcgtcaac ctgctgatgc
      601 gcttctacga gctcaaggga gggcggatcg cggtcgacgg cgtggacatc agggacgtgc
      661 cgcgaggccg gctcaggcgc gccttcggca tggtgctgca ggatgcctgg ctgttcggcg
      721 gcaccatacg cgacaatatc gcctacggcc gcgagggcgc gaccgaggac gagatcgtcg
      781 ccgcggcggt cgcggcccac gccgaccatt tcatccgcgt actgcccgac ggctacgaca
      841 ccgtgctgac cgaggacgcc accaacctgt cgcaaggcca gcgacagctc ctgaccatag
      901 cgcgcgcctt tttggccgat cccaggatac tcatcctgga cgaggcgacc agttccgtgg
      961 atacccgtac cgaactggtc gtgcgcaagg ccatggagcg cttgatgagg ggccgcacca
     1021 gctttgtgat agcgcaccgc ctatcgacga tacgcgatgc gcatactatc ctcgtgatga
     1081 acgagggccg catcgtcgag aagggctccc acgtggaact cctggccgcg gacggcttct
     1141 acgccgacct gtaccgcagc cagttcctcg gcgacatccc ggcggtcgcc gccatgatgc
     1201 ccgaggccca cttcggccgg ttcaccgcgc gcaagctgta catccacaac tgcggccacg
     1261 ccctgctcgc ctacgccggg tacctctacg gccacgagta cggctggcag
//
LOCUS       DAOOLN010000722         1307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_137061_length_1307_cov_3.866613, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000722 DAOOLN010000000
VERSION     DAOOLN010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137061_length_1307_cov_3.866613"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..631)
                     /locus_tag="PLW80_09075"
     CDS             complement(170..631)
                     /locus_tag="PLW80_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPB66704.1"
                     /translation="MPALRNALAPDRAAVGVKAADKREAIAALVDLLAAAGKAPDRDA
                     LLAAVLEREALAPTGLGDDCAIPHAQTDAVSETSVAVVRLAEPVGFDAPDGSPARLVF
                     LLAGPKDSAAVHLRLLSKLARLLSDPEFRRALLEAPDGAALAGLIIGADQA"
     gene            complement(633..980)
                     /locus_tag="PLW80_09080"
     CDS             complement(633..980)
                     /locus_tag="PLW80_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66705.1"
                     /translation="MKLLVLVLNDDKHLHGVMEAYVEAGVTGATILDSEGMGRFLAYQ
                     VPLFADFKDFMQGNKPSNKTILSVVRDERVLERLKDLVGRVIGGFSTPGAGVMFTVPV
                     DWFAGPERDEGDD"
     gene            1068..>1307
                     /locus_tag="PLW80_09085"
     CDS             1068..>1307
                     /locus_tag="PLW80_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66706.1"
                     /translation="MAIYETLAKAYDELFPPAPEAAAFIGRAIGSSEQKNEVDDRPWR
                     NGAGRAGAAAPTRGKPAIDLGCATGAHVAPPAGLGY"
BASE COUNT          208 a          442 c          455 g          202 t
ORIGIN      
        1 aagaggactt gaacctccat gcctctcggc actagcacct caagctagcg tgtctgccaa
       61 ttccaccatc gccgcgggca gttcgtcggg cggcccgggg gcgcgcctga cgggaggttt
      121 atacctgaaa ggcggcgcgc atgtcaagcc gggcctcggg tccggttcct caggcctggt
      181 cggcgccgat gatcaggccg gcaagggcgg cgccgtcggg ggcctcgagc agggctcgcc
      241 tgaactcggg atcggatagg agcctggcca gcttggacag gagccgcagg tgaacggccg
      301 ccgagtcctt ggggccggcg agcaggaaca cgagcctggc cgggctgccg tccggcgcgt
      361 cgaagcctac cggctcggca agtcgcacga cggctaccga ggtctccgag acggcgtcgg
      421 tctgggcgtg ggggatggcg cagtcgtcgc cgaggccggt cggagccagg gcctcgcgct
      481 cgagcacggc cgctaggagg gcgtcgcggt cgggcgcctt gccggcggcc gcgagcaggt
      541 cgacgagggc ggctatcgcc tcgcgtttgt cggccgcctt gacgccgacg gcggcgcggt
      601 ccggcgccag ggcgtttcgc agggccggca cgttagtcgt ccccctcgtc ccgctcggga
      661 ccggcgaacc agtcgaccgg cacggtgaac atgacgccgg cgcccggcgt cgagaagccg
      721 cctatgacgc gtccgacgag gtccttgagc cgctcgagga cgcgttcgtc ccgcacgacc
      781 gataggatgg ttttattgct cggcttgtta ccttgcatga aatccttgaa gtcggcgaac
      841 aggggcacct gataggccag gaaacgaccc atgccctcgg aatcgaggat agtggcgccg
      901 gtcacgccgg cctcgacgta ggcttccatc accccatgca gatgcttgtc gtcgttcagg
      961 accaatacga gcagtttcac cgtggcgcct ccgcgtccag tatcgacgaa tcggccgctt
     1021 tgtcaaacgc tgtttgcccg gaagccgccc gtgagtatct tggatccatg gcaatctacg
     1081 aaacgctcgc gaaggcctat gacgagctct tcccgccggc gcccgaagcc gcagccttca
     1141 tcggccgtgc cataggttcg tctgagcaga agaacgaagt cgacgatagg ccatggcgga
     1201 acggcgccgg ccgagccggc gccgccgcgc ctacgcgcgg caaaccggcc atcgacctcg
     1261 gctgcgcgac cggcgcccac gtcgccccgc cggccggcct cggctac
//
LOCUS       DAOOLN010000723         1307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_137108_length_1307_cov_2.681310, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000723 DAOOLN010000000
VERSION     DAOOLN010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137108_length_1307_cov_2.681310"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1307)
                     /locus_tag="PLW80_09090"
     CDS             complement(<1..>1307)
                     /locus_tag="PLW80_09090"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HPB66707.1"
                     /translation="IDKIGTYPWPRGLLAHGLEALAELGADYVVFDIEYLEKSPMTVD
                     DSYLRGGLRTEFDTAFEEIGSNMAEVFQSLANKRIPLADAGVYGGSLVEYIDETREYL
                     YSKTNLVAIENDAYLGQAMRLFGNAYTTLNIQKTRPNESFMSRMELVEDRFAYAGLSL
                     ERPMPKESAGFLVPIPEISGLSKGAGFTNVDIDSDGVRRRIKLVDFHDGKPYLQLSLA
                     PLLDRLGKPGLVYGRRELRLIGAKYPDGVRDVRIPLAMDGTMLIRWPKKTYIDSFDHI
                     AFHDLLTYRDEAGAFADAVRLLRANQGWTLGPGYAPVDACLAAWDESEAYRETALDSG
                     SAEDREAWLASRREYWLTVGEFVGMDYGDSVPALYDAARDADDPAYAELYDQMRDDFS
                     LLWNRASESYARHLRYDEHLRGKLEGAFCIVGWTSTGSTDFGV"
BASE COUNT          203 a          413 c          439 g          252 t
ORIGIN      
        1 gacgccgaaa tcggtcgagc cggtactggt ccagccgacg atgcagaacg cgccttcgag
       61 cttgccgcgc aggtgctcgt cgtagcgcag gtgcctggcg taggattccg aggcccggtt
      121 ccagaggagg gagaagtcgt cgcgcatctg atcgtacagc tccgcgtagg ccgggtcgtc
      181 ggcgtcccgg gccgcgtcgt acagggcggg gacgctgtcg ccgtagtcca taccgacgaa
      241 ctccccgacc gtcagccagt attcccgcct ggaggccagc caggcctcgc ggtcctcggc
      301 cgacccgctg tcgagtgcgg tctcgcggta ggcttcgctc tcgtcccagg ccgccaggca
      361 cgcgtcgacc ggcgcatagc ccggcccgag ggtccagccc tggttggccc tgagcagcct
      421 gacggcgtcg gcgaacgccc cggcttcatc gcggtaggtc agcaggtcgt ggaacgcgat
      481 gtggtcgaag ctgtcgatat acgtcttctt gggccagcgg atcagcatcg tgccgtccat
      541 ggccagcggg atgcgtacgt cgcgtacgcc gtccgggtac ttcgcgccga tcagccgcag
      601 ctcgcgccgg ccgtagacga ggccgggctt accgagcctg tcgaggaggg gcgccaggga
      661 cagctgtagg tagggcttgc cgtcgtggaa gtcgaccagc ttgatgcgcc tacgcacgcc
      721 gtccgagtcg atatcgacgt tcgtgaagcc cgcgcccttg gataagccgc tgatctcggg
      781 aatggggacg aggaagcctg cgctctcctt cggcatcggg cgttcgaggg acagcccggc
      841 gtaggcgaag cggtcctcga ccagctccat ccggctcatg aacgactcgt tcgggcgggt
      901 tttctggata ttgagcgtcg tgtaggcgtt gccgaatagg cgcatcgcct ggccaaggta
      961 cgcgtcgttc tcgatcgcta ccaggttggt cttgctgtag aggtattccc tggtctcgtc
     1021 gatatactcg accagcgagc cgccgtatac acctgcgtcg gccagcggaa tgcgcttatt
     1081 ggccagcgac tggaatacct cggccatgtt cgagcctatc tcttcgaatg ccgtatcgaa
     1141 ttcggttctg aggccgcctc gcaggtagct gtcgtccacc gtcatggggc tcttctcgag
     1201 gtactctata tcgaagacga cgtagtcggc cccaagttcg gccagcgcct cgagtccgtg
     1261 ggcgagcagt cccctgggcc aggggtaggt gccgatcttg tctatcg
//
LOCUS       DAOOLN010000724         1306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_137326_length_1306_cov_2.207034, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000724 DAOOLN010000000
VERSION     DAOOLN010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137326_length_1306_cov_2.207034"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PLW80_09095"
     CDS             <1..283
                     /locus_tag="PLW80_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66708.1"
                     /translation="SSSMYPVGAIVSSIEWKDKAYDDAYSETLASLARKRTEDPAFSA
                     EDARGVLKHLYIQDGNDWGGRGELQDITIRATIDAYERFIAEWVSDKAP"
     gene            complement(289..>1306)
                     /locus_tag="PLW80_09100"
     CDS             complement(289..>1306)
                     /locus_tag="PLW80_09100"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HPB66709.1"
                     /translation="KASGPTDAAALRRIVKDLVSSYSLVPIPVAILDGTMSFLYTNEP
                     FKRLLESFHASGKPTLIGAIGRFLDQATARDLYLAIKDPRRGYSWHGQIRLKAKDSSS
                     VIAKTTIMPFRPDASSDGRPMAWVAFLDDVTEERNALIRGLFSSLLEASKLKDNDTGR
                     HIERVNLYAERLASALYESGRWPEVDVDFVENIGFLAAMHDVGKIGTPDDILNKKGPL
                     DEFEWNIMKEHTINGAFILSSYPNPMAKQIAQSHHEWWNGTGYPYNLVGAMIPLPARI
                     VALADVYDALRMRRSYKLPFDHARASLLIVADGGKHFDPDVVEVFKRIGHDFDTIFND
                     NIDA"
BASE COUNT          236 a          389 c          421 g          260 t
ORIGIN      
        1 ctcttcgtct atgtaccccg taggagccat cgtgtcatcg atagaatgga aggacaaggc
       61 ctatgacgac gcgtactccg agacgctcgc cagtctagcg cgtaagcgga ccgaggatcc
      121 ggccttctcg gcggaggatg cgaggggcgt actcaagcac ctctatatcc aggacggcaa
      181 cgactggggc ggccgcggcg agcttcagga tataacgata cgggcgacga tagacgccta
      241 cgagcgcttc atcgccgagt gggtatcgga taaggcgccc tagcgtccct acgcgtcgat
      301 attgtcgttg aagatcgtgt cgaagtcgtg gccgatgcgc ttgaacacct cgacgacgtc
      361 gggatcgaag tgcttgcctc cgtcggcgac gatcaggagc gaggcgcgcg cgtggtcgaa
      421 gggcagcttg tagcttctcc tcatcctgag cgcgtcgtag acgtccgcca acgcgacgat
      481 gcgggccggt agcggtatca tcgcgcctac cagattatac ggatatccgg tgccgttcca
      541 ccactcatgg tgggactggg cgatctgctt ggccatgggg ttcgggtagc tcgacaggat
      601 gaaggcgccg ttgatcgtat gctccttcat gatgttccac tcgaattcgt ccagcggacc
      661 cttcttgttc aggatgtcat ccggcgtgcc tatcttgccc acgtcgtgca tggccgcgag
      721 gaagccgatg ttctccacga agtccacgtc gacctcgggc caccgcccgg attcgtacag
      781 ggcactcgcc agccgctcgg cgtagaggtt gacccgctcg atatggcgcc cggtgtcgtt
      841 gtccttaagc ttcgacgcct cgagcaggct ggagaagagc cctcggatga gagcgttgcg
      901 ttcctcggtc acgtcgtcga gaaaggctac ccaggccatc ggccgcccat cgctcgacgc
      961 gtcgggcctg aagggcatga tcgtcgtctt ggcgatcacg ctgctcgagt ccttggcctt
     1021 gagccggatc tggccatgcc agctgtagcc gcgcctcggg tccttgatcg ccagatagag
     1081 atccctcgca gtcgcctgat ccaggaagcg cccgatcgct cctatcaggg taggcttgcc
     1141 gctcgcgtgg aacgattcga gcagccgctt gaacggttcg ttcgtatata ggaacgacat
     1201 agtgccgtcg aggatcgcga cgggaatagg gacgagcgag tacgatgaga cgaggtcctt
     1261 gacgatgcgc cggagcgcgg cggcgtcggt cggcccggac gccttg
//
LOCUS       DAOOLN010000725         1305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_137463_length_1305_cov_2.727200, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000725 DAOOLN010000000
VERSION     DAOOLN010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137463_length_1305_cov_2.727200"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /gene="hflX"
                     /locus_tag="PLW80_09105"
     CDS             complement(<1..825)
                     /gene="hflX"
                     /locus_tag="PLW80_09105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPB66710.1"
                     /translation="MSELHDTAARPERAFLVGVRDVEVRAPEAESLLRELSGLASSLG
                     IETVGSAMVGLRARVASTMLGSGKVEEIIAAAKAEGADSIIFDKALSPVQQRNWEQLS
                     GLDVYDRAELIIDIFASRAKTREASLQVELARLRYSLPRLAHSYGDLHRQRGGRYGTK
                     GAGEQKIELDRRQIAKRIADIEEELVVVRRSRATQRKRRERLGLKRAAIVGYTNAGKS
                     SLLNAIASTADVVAEDKLFATLDPTTRRLDTSSGSVLVTDTVGFVRNLPHGLVEAFK"
     gene            1026..>1305
                     /locus_tag="PLW80_09110"
     CDS             1026..>1305
                     /locus_tag="PLW80_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66711.1"
                     /translation="MSIQIADIGKAVIETQYAVRGPIVARAQELERARRRIIYCNIGN
                     RQALGQAPLSWTRRLLALCEYPALAAAVPGAFPADAVEAAKTLLTGSKH"
BASE COUNT          193 a          464 c          443 g          205 t
ORIGIN      
        1 cttgaaggcc tcgacgaggc cgtggggcag gttgcgcacg aagccgaccg tgtcggtgac
       61 cagcaccgag cccgacgagg tgtccagccg gcgcgtcgtc gggtccagcg tggcgaacag
      121 cttgtcctcg gcgacgacgt cggcggtcga ggctatcgcg ttgagcagcg acgacttgcc
      181 ggcgttggta tagccgacga tggccgcccg cttgagcccg agccgttcgc gacgcttacg
      241 ctgcgtagcc ctcgagcggc gcacgaccac gagctcctcc tcgatatcgg ctatgcgctt
      301 ggctatctgc cggcggtcga gctcgatctt ctgctcgccg gcgcccttgg tgccgtagcg
      361 gccgcctcgc tgccggtgca ggtcgccgta ggagtgggcc aggcgaggca gcgagtagcg
      421 tagcctggcc agctccacct ggagcgacgc ctcccgggtc ttcgcgcggc tggcgaagat
      481 gtctatgatc agctcggccc ggtcgtacac gtccaggcca gatagttgct cccaattgcg
      541 ctgctgcacg gggctgagcg ccttgtcgaa gatgatggag tcggcgccct cggccttggc
      601 ggcggcgatg atctcctcga ccttgcccga gccgagcatc gtcgaggcga cgcgggcccg
      661 caggccgacc atggcgctgc cgaccgtctc gatgccgagg ctcgaggcca gcccggacag
      721 ctcgcggagg aggctctcgg cctcgggcgc ccgtacctcc acgtcgcgca cgccgacgag
      781 gaaggcccgt tccggacggg cggccgtatc gtgtaattcg ctcacgaacc tatactagcc
      841 tcgaaggccg ccctcggtcg agggccagtt cggctatacc cgttcgctcc gcggcccgcg
      901 ctccgcggct tcggttccga ggcgatacta tacgccggat acgacgcccc gccttggcgc
      961 ccgccccgag cgatgctatt atgggcgcgc gcggcccggc gccgcgcgat cgaaaaggag
     1021 tccccatgtc catccagata gccgacatcg gcaaggcagt catcgagacg cagtacgccg
     1081 tgcgcggccc catcgtcgcc agggcccagg agctagagcg ggccaggcgc aggatcatct
     1141 actgcaacat cggcaatcgc caggccctcg gccaggcccc gctgtcgtgg accaggcggc
     1201 tcctggcatt gtgcgagtat ccagccctgg cggccgccgt ccccggcgcc ttcccggccg
     1261 acgccgtgga ggccgccaag acgctgctaa ccggctccaa gcacg
//
LOCUS       DAOOLN010000726         1305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_137523_length_1305_cov_1.993600, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000726 DAOOLN010000000
VERSION     DAOOLN010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137523_length_1305_cov_1.993600"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLW80_09115"
     CDS             <1..186
                     /locus_tag="PLW80_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66712.1"
                     /translation="ISMAMPWDVMGGVARRSWARNEHAVATCAEYNEVYSGSGSITLP
                     YIADDRIVESALGGSGK"
     gene            183..1289
                     /locus_tag="PLW80_09120"
     CDS             183..1289
                     /locus_tag="PLW80_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HPB66713.1"
                     /translation="MNPAAGPTDSIVDVPGVSVGHATIADGGLQTGVTAVLPHAGNLF
                     RDKAPAACRVFNGFGKSVGLLQVEELGVVESPILLSNTFAMSACVDGALDLLLAENPD
                     IARETGSANVVALECNDGYLSDMRRRAVTREHVAEAIRLARSGSFAREASLRIGAGGP
                     SAGAFARGSVGAGRGMSCYGLKGGIGTASRVALADGRPYTVGTLTLTNFGRLSDFVVH
                     GRLLGKEIEALGGPAAARIRAAAIDGSGVGRPGDGSVIVVLTTDAPLDSRQLRRLCMR
                     AASGLARTGAVIGGGSGELALAFSTAYTVPHDPPPGGGPLPRATLHEDLLDPLFRAAT
                     DCVEAAVLDSLYSAETVVGRDGHERVALSELGLG"
BASE COUNT          175 a          462 c          465 g          203 t
ORIGIN      
        1 atctcgatgg ccatgccctg ggacgtgatg ggcggcgtcg cgcgccggtc ctgggcgagg
       61 aacgagcacg cggtggcgac ctgcgccgag tacaacgagg tgtattccgg ctccggttcc
      121 ataacgctgc cgtacatcgc cgacgaccgc atcgtcgagt ccgccctcgg aggctcgggg
      181 aaatgaaccc ggccgcgggc ccgaccgact cgatcgtcga cgtgcccggc gtctcggtcg
      241 ggcacgcgac catcgccgac ggcggcctgc agaccggcgt gaccgctgtg ctaccccatg
      301 cgggcaacct cttccgcgac aaggcgccgg ccgcctgccg ggtcttcaac ggcttcggca
      361 agtcggtcgg gctcttgcag gtcgaggagc tcggcgtcgt cgagtcgccg atcctgctat
      421 cgaatacctt cgcgatgtcg gcctgcgtcg acggcgccct cgacctgcta ctagcggaga
      481 accccgacat cgcccgggag accggtagcg ccaacgtcgt cgcgctcgag tgcaacgacg
      541 gctacctgtc ggacatgcgc cgcagggccg tgacgaggga acacgtagcg gaggctatac
      601 ggctcgcccg ctctggctcg ttcgcccggg aggcgtcgct gcggatcggc gccggcggcc
      661 cgtcggccgg cgccttcgcc aggggctccg tcggggccgg ccgcggcatg agctgctacg
      721 gactcaaggg cggcatcggc accgccagcc gcgtcgcgct ggccgatgga cggccgtata
      781 ccgtcggcac gctcacgttg accaactttg gccgcctgtc ggatttcgtg gttcacggta
      841 ggctcctcgg aaaggagatc gaggcgctcg gcggtcccgc ggccgcgcgg atacgcgcgg
      901 ccgctatcga cggctccggc gtcgggaggc ccggcgacgg ctcggtcatc gtcgtcctga
      961 ctacggacgc ccccctggac tcgcgccagc tacgaaggct gtgcatgcgc gctgcttccg
     1021 gcctggcacg aaccggcgct gtgatcggcg ggggctcggg cgagctggca ctggcgtttt
     1081 ccacggccta tactgttccg catgatccgc cgccaggggg cggcccgctc ccgcgggcga
     1141 cgctccacga ggatctgctc gaccccctct tcagggcggc caccgactgc gtcgaggcgg
     1201 ccgtgctcga ctcgctgtac agcgccgaga cggtcgtcgg ccgcgacggg catgagcgcg
     1261 tcgccctatc ggagctgggc ctcggctagg cacgggatgg cgttc
//
LOCUS       DAOOLN010000727         1302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_137935_length_1302_cov_3.822775, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000727 DAOOLN010000000
VERSION     DAOOLN010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137935_length_1302_cov_3.822775"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="PLW80_09125"
     CDS             <1..1162
                     /locus_tag="PLW80_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPB66714.1"
                     /translation="YALFVIVAPTRLTSLALPAFQACALAVGLACAVAIALAVARKRP
                     GAGLFGLGFLAIFGTAVYDIFVTAGVVHGAFASQVGMLAFLFALSLILTRRYAGAFSA
                     AEALSDDLARVNKSMERFVPKEFLGFLKKRSLEEIELGDSSSEEMAVLFVDVRSFSRM
                     AEDSTPEATFSFINEYLARVGPAVRDHGGFIDKYLGDGFMALFPGGAEPALRCALDVQ
                     ARVAAYNLEREAQGRKPIRVGVGMHAGRLMLGTIGESRRMDGTVVSDAVNVASRLEGV
                     SKEYGLGIAASECVLKELSDPTAFRMRFIGKVRVKGKAEPVSVFDIYEGDPDELRSRK
                     DLTRAAFERGIEAFHEHRLGDARTLFSQVLATIPDDGAALRYLNAIGERDPA"
BASE COUNT          197 a          450 c          440 g          215 t
ORIGIN      
        1 ctacgcgctc ttcgtgatcg tcgcgccgac caggctgacg tcgctggcgc taccggcctt
       61 ccaggcctgc gccctggccg tcggcctggc ctgcgccgtc gccatagccc tggccgtcgc
      121 gaggaagcgt ccgggcgccg ggctgttcgg cctcgggttc ctggccatct tcggcacggc
      181 ggtctacgac atattcgtca cggccggcgt cgtccacggc gccttcgcgt cccaggtcgg
      241 catgctcgcc ttcctcttcg cgctgtcgct gatcctgacg cggcgctacg ccggcgcctt
      301 ctccgcggcc gaggccctgt ccgacgacct ggccagggtg aacaagtcga tggagcgctt
      361 cgtcccgaag gagttcctcg gcttcctcaa aaagcgcagc ctcgaggaga tcgagctcgg
      421 cgactccagt tccgaggaga tggccgtgct cttcgtcgac gttcgctcgt tctccaggat
      481 ggccgaggac tcgacgcccg aggccacctt ctcgttcatc aacgaatacc tggccagggt
      541 ggggccggcc gtcagggacc acggcggctt catcgacaag tacctgggcg acggcttcat
      601 ggccctgttc ccgggcgggg ccgagccggc cctgcgctgc gccctcgacg tacaggcgag
      661 ggtggcggcc tacaacctgg aacgcgaggc ccaggggcgg aagcctatcc gggtcggcgt
      721 gggcatgcac gccggccgcc tgatgctcgg cacgataggc gagagccggc gcatggacgg
      781 cacggtcgtc tccgacgcgg tcaacgtggc cagccgtctc gagggcgtct ccaaggaata
      841 cggcttgggc atcgcggcca gcgagtgcgt gctcaaggag ctgagcgacc cgaccgcgtt
      901 caggatgcgc ttcatcggca aggtcagggt caagggcaag gccgagccgg tgtcggtctt
      961 cgatatctac gagggcgacc cggacgagct gcgttccagg aaggacctga cccgagcggc
     1021 cttcgagcgc ggcatcgaag ccttccatga gcaccgcctc ggcgacgccc gcacgctatt
     1081 cagccaggta ctggcgacca tccccgacga cggggccgcc ctgcgctacc tgaacgccat
     1141 cggggagcgc gacccggcct agcgcgccgg gtcgcgccgc gctcgctata gcgcgaagtt
     1201 accgttcgtg aagacgggga cttcggagcc gtcggccatc acgccgacta tctcgagatc
     1261 cttggaaccg accatgaagt cgacgtggat cagcgagtcg tt
//
LOCUS       DAOOLN010000728         1301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_138159_length_1301_cov_2.670144, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000728 DAOOLN010000000
VERSION     DAOOLN010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138159_length_1301_cov_2.670144"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..731
                     /locus_tag="PLW80_09130"
     CDS             159..731
                     /locus_tag="PLW80_09130"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPB66715.1"
                     /translation="MRGGDDAALAFEALWDAYYRRLMVFAAAYRGLPSAERHDAAADA
                     LIAAFGGLRGFDPRRPLSPWVYRIAANHFSDAARRARRESTIEVGQADSAGSDDGATG
                     GYRGPAVVEPATSGDHEADSVERDLVERCEAAIAALPEADRRVAMLRFYEGFSAADVG
                     RALGMPAGTVRWRVGVIRAAIRAATGEDRP"
     gene            728..1060
                     /locus_tag="PLW80_09135"
     CDS             728..1060
                     /locus_tag="PLW80_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66716.1"
                     /translation="MTADERVRAAFESEARRVEAREPSSVETRRALAAARLMPISATR
                     RRLGAGELIPVVAMAAAAFAVAAIDLGSMQRLRPLAGVLAAAVPDSAGERFVDFVLEA
                     GESYRSAN"
     gene            1092..>1301
                     /locus_tag="PLW80_09140"
     CDS             1092..>1301
                     /locus_tag="PLW80_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66717.1"
                     /translation="MLKRIVFPMLVVVAVIALTMFVCAVTPWMYIDIASFILVPIGSL
                     LFMGLAFGWKASGAAFSSAWRPNASR"
BASE COUNT          198 a          382 c          483 g          238 t
ORIGIN      
        1 aaactccgcc aacaccgagg cggttcgggg cgtacaataa gcgtatgcat gatagtgacg
       61 gcgcagaaga aggcgctagc gagtgttgta cgactgctgg ggctcggcga gctcgagcgg
      121 cgggagagcc gagctcggcg gagcttcgag acgcggccat gaggggtggg gacgatgcgg
      181 cgctggcctt cgaggcgctg tgggacgcct attataggcg gctgatggtc ttcgccgccg
      241 cttaccgggg acttcctagc gccgaacggc acgacgcggc cgcggacgcg ttgatcgccg
      301 cgtttggggg cctacgaggc ttcgacccga ggaggccttt gtcgccgtgg gtataccgca
      361 tcgcggccaa tcacttctcg gacgcggcca ggcgggctcg acgagagtcg acgatagagg
      421 tcgggcaggc tgattcggcc ggttccgatg atggcgctac agggggctat cgaggtccgg
      481 ccgtagtcga gcccgcgacg agcggagacc atgaggcgga ctctgtcgag cgtgacctgg
      541 tcgagcgctg cgaagccgct atagcggcgt tgcccgaggc ggatcggcga gtcgccatgc
      601 tacgcttcta cgaaggcttc agcgccgccg acgtcggcag ggcgctcggt atgccggccg
      661 gtacggtgcg ttggcgcgtc ggcgttattc gcgcggccat ccgcgcggct acgggggagg
      721 ataggccatg acggcggacg aacgggtacg ggcggctttc gagtcggaag cgcgccgagt
      781 cgaggctcgg gagccttcga gcgtcgagac gaggcgggcc ttggccgcgg ccaggcttat
      841 gcctatttcg gcgacgcgac gacggctggg cgcgggcgag ctgataccgg tcgtcgcgat
      901 ggccgctgcg gctttcgccg tggcggccat cgatcttggt tcgatgcagc gcctgcgccc
      961 cctcgccggg gtgcttgccg ccgcggtgcc tgacagcgcc ggggaacgct tcgtggattt
     1021 cgtactcgag gccggcgagt cgtatcggtc agcaaactag tcatcttcaa cgcaaattcc
     1081 aggaggaaag agtgctcaag cgaatcgtgt tcccgatgct cgtcgtcgtc gccgtgatcg
     1141 cgctcaccat gtttgtgtgc gcggtgacgc cttggatgta tatcgacatc gcgtcgttca
     1201 tcctcgtgcc tatcggctcc ctgctcttca tggggctggc tttcggctgg aaggcgagcg
     1261 gggcggcttt ctcgtcagct tggagaccca acgcctcgag g
//
LOCUS       DAOOLN010000729         1300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_138348_length_1300_cov_2.375100, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000729 DAOOLN010000000
VERSION     DAOOLN010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138348_length_1300_cov_2.375100"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="PLW80_09145"
     CDS             complement(<1..878)
                     /locus_tag="PLW80_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB66718.1"
                     /translation="MKKVLTALAIALCVVSVTTAQVKNGPIVDKVIYTVSMDQSIAMK
                     DIVEGKADVFFQAVPANILRTLSEAEKAKLDIYAVPSGSWSLMLNPIPNKAPYTWTKS
                     DGVTEFNPFAIREVRYALNWLINRKKLVDEILLGDGEPMFTPMTPGQPGTYRYNLIAS
                     QLGMTATGNERKAIADIDAAMTAASQLPENKGKLVKKGQFWQYNGQDVTIKFLIRVDD
                     PTGRLPAGRYIADQIEKAGIKVDRQERDRSGIRIAYYSNPADLQFHMYTEGWGAGATR
                     AWWDVTISQMYAPYYG"
     gene            complement(1123..>1300)
                     /locus_tag="PLW80_09150"
     CDS             complement(1123..>1300)
                     /locus_tag="PLW80_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HPB66719.1"
                     /translation="VLKVYAEPLLRSVKPMDPEYCEAKRLLNFLAFFAPIPAQYVPQD
                     SLVREFIGESSFKY"
BASE COUNT          230 a          355 c          439 g          276 t
ORIGIN      
        1 tagccgtagt agggagcgta catctggctg atcgtcacgt cccaccaggc gcgggtggcg
       61 ccggcgcccc agccctcggt gtacatatgg aactggagat cggcggggtt cgagtagtag
      121 gcgatgcgga taccggagcg gtcgcgctcc tggcggtcca ccttgatgcc ggctttctcg
      181 atctggtcgg cgatgtagcg gccggccggg aggcggcccg tcgggtcgtc gacgcggatc
      241 aggaacttga tcgtgacgtc ctggccgttg tactgccaga actggccctt cttgaccagc
      301 ttgcccttgt tctcgggcag ctgcgaagcg gcggtcatcg cggcgtcgat gtcggcgata
      361 gccttgcgct cgttgccggt ggcggtcatg ccgagctggg aggcgatcag gttgtagcgg
      421 taggtgccgg gctggccggg cgtcatcggg gtgaacatcg gctcgccgtc gccgagcagg
      481 atctcgtcga cgagcttctt gcggttgatg agccagttca gcgcgtagcg gacctcgcgg
      541 atggcgaacg gattgaactc cgtgacgccg tcgctcttgg tccaggtata cggggccttg
      601 ttcgggatcg ggttgagcat gagcgaccac gatccggacg gcacggcgta gatgtccagc
      661 ttggccttct cggcctcgct gagcgtcctg aggatgttgg cggggacggc ctggaagaag
      721 acgtcggcct ttccttccac gatgtccttc atggcgatgg actgatccat gctgacggta
      781 tagatcacct tgtcgacgat cgggccgttc ttgacctggg cggtcgtcac ggacacgacg
      841 cacaaggcta ttgccagcgc ggttagtacc ttcttcatgt aaaacctcct tacatgagaa
      901 ctgaaaagat agggcattgt gtagcatctt cgtcgatttg taaagaatcg agttgcgcgg
      961 cgacgtaaaa tttaggcgat ggcgatgcga aagttaaatt tcgatattat ctatagttac
     1021 gaggctatat atagaagcgc tcccgtatcg ccctggagcg acgatggacg cgttctggag
     1081 agcctcgagc gacggggcgt cgatcgcccc gtcgctcggg actcagtatt taaacgagct
     1141 ctccccgatg aactcgcgca cgaggctgtc ctggggcacg tactgcgccg ggatgggcgc
     1201 gaagaacgcc aggaagttta agaggcgctt ggcctcgcag tactcggggt ccatcggctt
     1261 gaccgagcgc aggagcggct ccgcatagac cttgagcacg
//
LOCUS       DAOOLN010000730         1300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_138413_length_1300_cov_1.685944, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000730 DAOOLN010000000
VERSION     DAOOLN010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138413_length_1300_cov_1.685944"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="PLW80_09155"
     CDS             complement(<1..906)
                     /locus_tag="PLW80_09155"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPB66720.1"
                     /translation="MLKRIGIALILAVSLAAATTEAQDRVDLVVLLDSSQSMFQYYNQ
                     VVDFVLSQTVKEYMRFGDAFHLVSFSDTTQVEIAQVLRTEKDIRSAVARLYLLYPLGR
                     NTDLVMALRNVYQYVADLPEGSAKHILLITDGMHSPAAGTQFAGLGPDEVRAELERTA
                     GRVRERGWTLRIVRVPFGSGASAEASAQGQGSVAGATGTAGYGEAGSNAPGAGDYLAD
                     LAAAAGTAVSSFDPANASATVEDTIDLPRISFPAGLGATDYALSIPVEIGNGSSRELR
                     VELTRLLLDDGTDILREKAFATVPAG"
     gene            complement(960..>1300)
                     /locus_tag="PLW80_09160"
     CDS             complement(960..>1300)
                     /locus_tag="PLW80_09160"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HPB66721.1"
                     /translation="YKAAKEKQDELNAKVGKLKNEIERAQIFDAGSITTGKISFATEV
                     LLHNDLDDADEIYRILGPWESDPSNHVISYLSPLGKRLLNHKVGDKLSFVIHERKFSY
                     TVKAITAIDL"
BASE COUNT          216 a          395 c          433 g          256 t
ORIGIN      
        1 tccggcgggc acagtcgcga aggccttttc acgtaggatg tccgtaccgt cgtcgaggag
       61 caatcgggtc agctcgaccc gcagctcccg ggaggagccg ttccctatct cgacaggtat
      121 ggataacgcg tagtccgttg caccgagccc ggcggggaac gatatccgcg gaaggtctat
      181 cgtatcctcg accgtggcgg acgcgttggc cggatcgaag gaactgacgg ccgtgcccgc
      241 ggcggcggcc aggtcggcta ggtagtctcc cgcacccggg gcgttcgaac cggcctcccc
      301 gtagcctgcg gtaccggtgg ctcccgctac ggagccctgg ccctgggcgg aagcctcggc
      361 cgaggcgccg ctgccgaatg gcacacggac gatgcgcagc gtccaaccgc gttcgcggac
      421 cctgccggct gtgcgctcga gctcggccct gacctcgtcc ggcccgagac ccgcgaactg
      481 ggtgcccgcg gcgggggagt gcataccgtc ggtgatcagg agtatgtgct tcgcgctacc
      541 ctcgggcagg tcggccacgt actggtagac gttccggagg gccatcacga ggtcggtgtt
      601 gcgcccgagc gggtacagca ggtacagcct ggcgacggcg ctcctgatat ccttctcggt
      661 tctcaggacc tgggcgatct cgacctgggt agtatcggag aaggatacca ggtggaaggc
      721 atcgccgaag cgcatgtact ccttgaccgt ctgcgacagc acgaagtcga cgacctgatt
      781 gtagtactgg aacatgctct gcgacgaatc gagcaggacg accaggtcga cgcgatcctg
      841 ggcctccgtg gtagccgcgg ccagagagac ggccaggatg agcgctatcc cgatacgttt
      901 cagcatgatc gattccttcg cttggtcacg cgcccggatc cagcgggcgc gtgaccgaat
      961 tataggtcga tcgcggtgat cgcctttacc gtatacgaga atttcctctc gtggatgacg
     1021 aatgacagct tatcgccgac cttgtggttc aggaggcgct tgccgagcgg ggacaggtac
     1081 gagatgacgt ggttgctcgg gtcggattcc cagggaccga gtatcctgta gatctcgtcg
     1141 gcgtcgtcga ggtcgttgtg gagcagcacc tcggtggcga aggatatctt gccagtcgtg
     1201 atgctacccg catcgaagat ctgcgcccgc tcgatctcgt tcttgagttt gcctaccttg
     1261 gcgttgagct cgtcctgttt ctccttggcg gccttgtact
//
LOCUS       DAOOLN010000731         1298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_138611_length_1298_cov_3.684634, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000731 DAOOLN010000000
VERSION     DAOOLN010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138611_length_1298_cov_3.684634"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /gene="flgK"
                     /locus_tag="PLW80_09165"
     CDS             complement(<1..284)
                     /gene="flgK"
                     /locus_tag="PLW80_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255182.1"
                     /note="with FlgL acts as a hook filament junction protein
                     to join the flagellar filament to the hook; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HPB66722.1"
                     /translation="MQSTFAGIELGKRGLVAHNQGLQTIGHNLTNAATEGYSRQRVEL
                     GTMEPLYAPHLNREETPGQVGQGVVAVRVERMRDELLDRRIVAQTGGLGY"
     gene            complement(299..796)
                     /locus_tag="PLW80_09170"
     CDS             complement(299..796)
                     /locus_tag="PLW80_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66723.1"
                     /translation="MNIEALMTFIARVGEQIEALDAFVEDQRAVAGAVRARDWPALER
                     ALERAGASAGRVSSAEALRVAAWESLVFGLGLPADSSVFRASLLLPLEFRSTLNESYR
                     SLRLSAMRARIENDALSGFVGSAASTLREAMEAAFPERKGRIYGRTGLPRHAGSGAMI
                     LDTAL"
     gene            926..>1298
                     /locus_tag="PLW80_09175"
     CDS             926..>1298
                     /locus_tag="PLW80_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB66724.1"
                     /translation="MFKALTNLYWLARGVRVFALVGGSGTGKSFRAKLVAQKFGIDMI
                     IDDGLLISGDAIVAGKSAKREKLYMAAVKTALFHEKAHRDEVARVLQKTRWKKILIIG
                     TSEKMTMKIAERLQIPYPAKTI"
BASE COUNT          222 a          433 c          431 g          212 t
ORIGIN      
        1 cagtagccga gcccgccggt ctgcgcgacg atgcggcggt cgagcagctc gtcgcgcatg
       61 cgctcgacgc gcaccgcgac cacgccctgg ccgacctggc ccggggtctc ctcgcggttc
      121 aggtggggcg cgtacagcgg ctccatcgtg ccgagctcga cccgctggcg cgagtagccc
      181 tcggtcgcgg cgttcgtcag gttatggcct atggtctgga gcccctggtt atgcgcgacg
      241 aggccgcgct tgcctagttc aatgccagcg aaagtcgatt gcacgatagc ctcctaaatc
      301 agagagccgt gtccaggatc atggcgcccg agccggcgtg cctcggcagg ccggtccggc
      361 cgtagatgcg gcccttccgc tccgggaagg ccgcttccat cgcctcgcgc aatgtcgagg
      421 cggcgctgcc gacgaagccc gacagcgcgt cgttctctat cctggcgcgc atggccgaca
      481 gcctcaggga gcggtaggac tcgttcagcg tcgagcggaa ttcgagcggc agcaggagcg
      541 aggccctgaa cacgctcgag tcggccggca ggcccagtcc gaagacgagg ctctcccagg
      601 ccgcgacgcg gagggcttcc gcggacgata cgcgaccggc cgaggcgccg gcccgttcga
      661 gggctctctc gagcgccggc cagtccctgg ccctgaccgc gccggccacg gcccgctggt
      721 cctcgacgaa cgcgtcgagg gcctctatct gctccccgac tctggctatg aatgtcatca
      781 acgcttcgat gttcatatct accctcgctt cattatcggt actggaaacg aggagtttac
      841 gccgatatag taggtaggcc cgcgtacggg ggcgaatttc tgatggggga cgggggcgct
      901 agccgcttcc gggctcgagg gatcgatgtt caaagcgctg accaacctgt attggctcgc
      961 ccgcggcgtg cgcgtcttcg cgctcgtcgg cggtagcggc accggcaaga gcttccgcgc
     1021 caagctcgtc gcccagaagt tcggcatcga tatgatcatc gacgacggac tgctgatcag
     1081 cggcgacgcc atcgtggccg gcaagtcggc caagcgcgag aaactgtaca tggcggcggt
     1141 caagaccgcc ctgttccacg agaaggccca tcgcgacgag gtggcccgcg tgctccagaa
     1201 gacgcgctgg aagaagatcc tcatcatcgg cacgtcagag aagatgacga tgaagatcgc
     1261 cgagcgcctc cagataccgt acccggccaa gaccatcg
//
LOCUS       DAOOLN010000732         1297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_138813_length_1297_cov_3.201288, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000732 DAOOLN010000000
VERSION     DAOOLN010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138813_length_1297_cov_3.201288"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..>1297)
                     /gene="lysS"
                     /locus_tag="PLW80_09180"
     CDS             complement(176..>1297)
                     /gene="lysS"
                     /locus_tag="PLW80_09180"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPB66725.1"
                     /translation="ISVHAKRMVLLTKAIEPLPEKFHGLKDPEQRYRRRYLDMVMNRE
                     VMDTFRKRTQVIRAVREYLDNIGYIEVETPILSTIASGAAARPFVTKSNALNLQLYLR
                     IATELYLKRCIVGGMERVYEMGKDFRNEGIDIRHNPEFTMIELYQAYADYNDMMELCE
                     NLCSYAATKVCGSTKVSYQGTEIEFAAPWRRLTMVDAVKTYGGPDFSLVKSDEEAREL
                     AKAHGLEEELKKKLKDCTKGDILNAAFEKYAEKELIQPTFIMDYPTDISPLTKQKPGD
                     PTMTERFEAFVYGRELGNAYSELNDPIVQMERFTQQEREKELGDDEAYALDEDFVSSL
                     EIGMPPTGGMGIGIDRLIMFLTDSYSIRDVILFPTMKPL"
BASE COUNT          202 a          397 c          426 g          272 t
ORIGIN      
        1 ccagcgaagc gtagacgcca gccggcgtcc agcgaagcgt agacgccccc gccaaagata
       61 acagcgatcg ccggcaagct cggcgatcgc tgttatctct cggccggctt aacttgcggc
      121 ccctcgggcc gtcaggttga agccggcctc tatgaccctg cgcgcagcgt cagggtcata
      181 ggggcttcat ggttggaaat aatatcacgt ccctgatgct gtacgagtcg gtcaggaaca
      241 tgatcaggcg gtcgatgccg atgcccatgc cgccggtcgg gggcatgccg atctcgaggc
      301 tggagacgaa gtcctcgtcc agcgcgtagg cctcgtcgtc gccgagctcc ttctcgcgct
      361 cctgctgggt gaagcgctcc atctggacga tggggtcgtt gagctcggag tacgcgttac
      421 ccagctcgcg gccgtagacg aaggcctcga agcgctcggt catcgtcgga tcgcccggct
      481 tctgcttggt caaaggcgag atgtcggtcg ggtagtccat gatgaaggtc ggctggatca
      541 gctccttctc ggcgtacttc tcgaacgcgg cgttgaggat atcgcccttg gtgcagtcct
      601 tgagcttctt cttgagctcc tcctcgaggc cgtgggcctt ggccagctcg cgggcctcct
      661 cgtcgctctt cacgagcgag aagtcggggc cgccgtaggt cttgaccgcg tcgaccatgg
      721 tcagccggcg ccacggcgcg gcgaactcga tctcggtgcc ctggtagctc accttcgtcg
      781 acccgcagac cttggtggcc gcgtaggagc agaggttctc gcacagctcc atcatgtcgt
      841 tgtagtcggc gtaggcctgg tacagctcga tcatcgtgaa ttccgggttg tgccggatgt
      901 cgatgccctc gttgcggaag tccttgccca tctcgtagac gcgttccatg ccgccgacga
      961 tgcagcgctt gaggtacagc tcggtggcga tgcgcaggta cagctgcagg ttgagcgcgt
     1021 tggacttggt gacgaagggc ctggccgcgg cgccgctcgc gatcgtagag agtatcggcg
     1081 tctcgacctc gatgtagccg atgttatcca ggtactcgcg gacggcgcgg atgacttggg
     1141 tgcgcttcct gaaggtgtcc atcacctcgc ggttcatcac catgtccagg tagcggcggc
     1201 ggtagcgctg ctccgggtcc ttgaggccgt ggaacttctc cggcagcggc tcgatcgcct
     1261 tggtaagcag caccatccgc ttggcgtgca cggatat
//
LOCUS       DAOOLN010000733         1297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_138924_length_1297_cov_1.864734, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000733 DAOOLN010000000
VERSION     DAOOLN010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138924_length_1297_cov_1.864734"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(610..735)
                     /locus_tag="PLW80_09185"
     CDS             complement(610..735)
                     /locus_tag="PLW80_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66726.1"
                     /translation="MTTKPSARALSISIIRSWMAAIASGLVIVVRPSARRSRRSS"
     gene            complement(754..>1297)
                     /locus_tag="PLW80_09190"
     CDS             complement(754..>1297)
                     /locus_tag="PLW80_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66727.1"
                     /translation="AHLKYTAVAERKSEIAAAKDEFTLRERICPEIAKDHRIDDLGRE
                     ALAQALLSAFTSLGSKYPIAQCRKLLAEKRPEKYAGGSVPDWLKDWVYVSDDDKFFRM
                     DSDEWVSMQGFNARFNRELPVGEDGTINKSAAWVALENYAIPTLTRALYLPWADKMFD
                     LDGVRCVNSYRKSSVPEATR"
BASE COUNT          252 a          391 c          348 g          306 t
ORIGIN      
        1 taaggaatag agttaagaca gaacaagatt ggtcttttac ttaggctact cttcatcctg
       61 tccatcttct tccatcatgg aaaatcatgt tcccgtcttc gcctaggaat agggttaaga
      121 cggaacagga tgatcaggat taaggcagga tgatcaggat aagtcctaaa cctggacttt
      181 gctttatctt gttcatcttc ttccatcctg taccatcatg ttcccgtctt cagactaaaa
      241 accctcttcc ttggcgtctc ggcgcgccgt atcgtcaaat tcgatgattt ctataatttt
      301 tttacgaatt ctattcaagt ttttagatta tatacgccga tacaaagagt gtactccgga
      361 gcgcggtctt gctactataa cggcagaaat gccgggcgac cccttaggag agccgcaatc
      421 accactcgta cggatcggtc gccgggttct ataccataac gcagctttgt tcactgttcc
      481 actggctagc cgacggcggc caccgtggaa gggctgacga atgcctctga gcattacagc
      541 gcttcgcaac cacctcgcct cgtgcgagaa accctactac gttcgctcgt cgaacgcggg
      601 caccatcgcc tacgacgatc tcctcgatcg cctggcagaa gggcgcacca cgatcaccaa
      661 gccggacgcg atggcggcca tccagctcct gatgatggag atggacaggg ccctggcgga
      721 cggcttcgtc gtcaagctgc ctttcggcgc cttctacctc gtcgcctcgg gcacgctgga
      781 cttgcggtac gagttcacgc accggacccc atccaagtcg aacatcttgt ccgcccaggg
      841 caggtacagc gccctggtca gcgtggggat cgcgtagttc tccagcgcca cccatgccgc
      901 gctcttgttg atcgtgccgt cctcgcccac gggcagctcg cggttgaacc tggcgttgaa
      961 gccctgcatg gagacccact cgtcgctgtc catgcggaag aacttgtcgt cgtccgagac
     1021 gtagacccag tccttgagcc aatctggcac cgatccccct gcgtattttt ccgggcgttt
     1081 ttcggccaag agcttgcggc actgggcgat ggggtacttg ctacccaggc tggtgaacgc
     1141 ggagagtagt gcctgcgcca gcgcttcgcg cccgaggtca tcgatccggt ggtcctttgc
     1201 gatctcagga cagatgcgct cgcgcagggt gaactcgtcc ttggccgccg cgatctcgct
     1261 cttgcgctcg gccacggcgg tgtacttcag gtgggcg
//
LOCUS       DAOOLN010000734         1296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_139004_length_1296_cov_3.275584, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000734 DAOOLN010000000
VERSION     DAOOLN010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139004_length_1296_cov_3.275584"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..483)
                     /gene="rpsT"
                     /locus_tag="PLW80_09195"
     CDS             complement(214..483)
                     /gene="rpsT"
                     /locus_tag="PLW80_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPB66728.1"
                     /translation="MATKFSSAEKRHKQDEKRRVQNKKVKSSVRTAAKRVVTAAHAKD
                     KDLAEASLKDMIKQIDTAARKGIIKKNAAARKKSRMQRLVNVLNA"
     gene            639..>1296
                     /locus_tag="PLW80_09200"
     CDS             639..>1296
                     /locus_tag="PLW80_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPB66729.1"
                     /translation="MGTILLALTSLTIALAYEPLAYACRFLPARLRRERVAWVALAAM
                     DRFVACARAYIGMRLRYERAGFDAPERCIVVSNHQSLLDIPVLMHYFRAERRLLFVAK
                     KELGYGIPLISSVLRVGGHALIDRGSNPTRAMRELGAFARRSGAPGFWPSVFPEGTRS
                     KDGSLGKFHAAGLRRIADFAPAPLLVVAVDGGYKATKLSSLLTGSVGFEYRVKALGLI
                     E"
BASE COUNT          209 a          388 c          409 g          290 t
ORIGIN      
        1 ttcttgatct cttcgaacag gccgtcgatg aggatgtgta tgtctttacg gccgaggttc
       61 gagcgctcgt ggagagcgtc tatcagttcg gctttcgtga cttttctagc catgcgacga
      121 ctcctgctga atcggaccgt ccggggaccc tatctcggag aggcccgaag gcctccctat
      181 accgatggag cggccggccg cggggtaagg cgattaagcg ttgaggacgt tgacgagacg
      241 ctgcatcctg gacttcttgc gggcggcggc gttcttcttg atgatgccct tgcgcgcggc
      301 ggtgtcgatc tgcttgatca tatccttgag agaggcctcg gcgaggtcct tatccttggc
      361 atgggcggcg gtgaccacgc gcttggcggc ggtgcggacg ctgctcttga ccttcttgtt
      421 ctggacgcgt cgcttctcgt cctgcttgtg ccgcttctcg gcggatgaaa atttggtagc
      481 caatctatcc tccattcgga atgtcgcaac tctatgtcta ttagacagga attaacgtcg
      541 gtagaagaaa ttaccagatc ggctagagta tgtcaaggat atcctgggcc agcccgcgct
      601 tattgccgag cgaccggcga accgatatag tcgttcccat gggaaccatc ctgctagccc
      661 tgacctcgct gaccattgcc ctggcgtacg agcctctcgc ctacgcgtgc cgtttcctgc
      721 ccgcgcgcct tcgccgcgag cgggtcgcct gggtcgcgct cgcggcgatg gaccgtttcg
      781 tcgcctgcgc gcgcgcctat atcggtatgc gcctgcgcta cgagcgggcc ggcttcgatg
      841 ctccggaacg ttgcatagtc gtatcgaacc accagagcct gctcgatata cccgtgctca
      901 tgcattactt cagggctgag cgccgccttt tgttcgtggc gaagaaggaa ctcggttacg
      961 gcataccgct gatatcgagc gtgctcagag tcggcggaca cgctctgatc gaccggggga
     1021 gcaacccgac cagggcgatg cgcgaactcg gcgctttcgc gaggcggagt ggagcgcctg
     1081 ggttctggcc ctcggtcttc ccggaaggca cccgctccaa ggatggctcg ctcggcaaat
     1141 tccacgctgc cggcctcaga cgcatcgccg actttgctcc ggctcccctg ctggtcgtgg
     1201 ccgtggacgg cggctacaag gcgaccaagc tgtcatcgtt gctgacgggg agcgtggggt
     1261 tcgagtacag ggtcaaggcg ctcggactga tcgagc
//
LOCUS       DAOOLN010000735         1296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_139005_length_1296_cov_3.270749, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000735 DAOOLN010000000
VERSION     DAOOLN010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139005_length_1296_cov_3.270749"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PLW80_09205"
     CDS             complement(<1..408)
                     /locus_tag="PLW80_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HPB66730.1"
                     /translation="MSRFIVRRFLSVIPTLFIIVTLSFFLIRLAPGGPFSGEKKLPPE
                     IMANIMRKYHMDEPLVKQYFRYMGDVLQFDLGPSYRYKDQTVNDLIGNAFPVSVMLGT
                     MALGTALLLGTIVGITSALKQNKWQDYAAMSVAV"
     gene            complement(651..>1296)
                     /locus_tag="PLW80_09210"
     CDS             complement(651..>1296)
                     /locus_tag="PLW80_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB66731.1"
                     /translation="MQELVDKVTKGGQLATSSMVPAMEGYTPAKGPTFNVEEARKLLA
                     EAGFPDGKGFPKTPILYNTSDGHKIIAEWVQESWKKNLGIEVTLLNKEWKTFLDTRSN
                     AHDFDIARAGWVGDYLDPNTFLDMFIIGSGNNDGLYANPEYDALVKKAGTMKAGPERM
                     ATLQAAEALLIAKDQAVIPFYHYVEQDLIDTTKWDGWYANPLGVHDWKYIGPKK"
BASE COUNT          244 a          361 c          408 g          283 t
ORIGIN      
        1 cacggccacc gacatcgccg cgtagtcctg ccacttattc tgcttgagcg ccgaggtgat
       61 gccgacgatg gtgccgagca acagcgcggt gccaagggcc atcgtgccga gcatcacgga
      121 gaccgggaag gcattgccta tcaggtcgtt gacggtctga tccttgtatc gataggacgg
      181 accgaggtcg aactgcagta catcgcccat atagcggaag tactgtttaa caagcggctc
      241 gtccatgtgg tatttccgca tgatgttcgc catgatttcc ggcggcagtt ttttctcgcc
      301 ggagaaaggc ccgccggggg cgagcctgat caggaagaag ctgagcgtca cgatgatgaa
      361 cagggtcggt atgacgctca ggaatcttcg gacgatgaat ctagacatcg ttgcctcgct
      421 tgaaaagggt acaggccatt cgaggatagt cgaacgacct accgcggcgc ccctatccgc
      481 taggacggct acgccgcgag aaagccagta tacacccatg taccgggatt tgccagaaat
      541 ttcttagcta atcgtaatta tttgaaatgt cgttttgtta tttgaaagta atagctacat
      601 cggctaaaaa aggcccggcg ccaaagcgcc gggcccgttg gctcaaagcc ttacttcttg
      661 gggccgatgt acttccagtc gtggacgccg agcgggttgg cgtaccagcc gtcccacttg
      721 gtcgtgtcga tcaggtcctg ctccacgtag tggtagaacg ggatgaccgc ctgatccttg
      781 gcgatcagca gagcctcggc cgcctggagg gtggccatgc gctcgggacc ggccttcatc
      841 gtgccggcct tcttcacgag ggcgtcgtac tcggggttcg cgtacaggcc gtcgttgttg
      901 ccggagccga tgatgaacat gtcgaggaag gtgttcgggt cgaggtagtc gcccacccag
      961 ccggcgcgcg cgatgtcgaa atcgtgggcg ttcgagcggg tgtcgaggaa ggtcttccac
     1021 tccttgttga gcagggtaac ctcgatgccg agattcttct tccaggactc ctgcacccac
     1081 tcggcgatga tcttatgacc gtcgctggtg ttgtacagga tcggggtctt ggggaagccc
     1141 ttgccgtccg ggaagccggc ctcggcgagg agcttgcggg cctcttccac gttgaaggtc
     1201 ggacccttgg cgggcgtgta gccttccatg gccgggacca tcgagctggt ggccagctgg
     1261 ccgcccttgg ttaccttgtc gacgagctcc tgcatg
//
LOCUS       DAOOLN010000736         1292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_139695_length_1292_cov_3.506063, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000736 DAOOLN010000000
VERSION     DAOOLN010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139695_length_1292_cov_3.506063"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1292)
                     /gene="dnaE"
                     /locus_tag="PLW80_09215"
     CDS             complement(<1..>1292)
                     /gene="dnaE"
                     /locus_tag="PLW80_09215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPB66732.1"
                     /translation="GMQGILRQAKPSSVEDLIALNALYRPGPMDNIPQFIDSKWGRTP
                     ITYPHPSLEKYLKETYGVIVYQEQVMQVAQEVAGYSLGRADLLRRAMSKKKVEVLEKE
                     KAPFIEGALKRGYTKEKAQEIFDILVPFAGYGFNKSHAAAYSVVAYRTAWLKANYPAE
                     YMAANLTNEISNTDKLTQYISEARSMGIGILAPDINRSEATFSVSEGKIVYGLIGVKG
                     VGEAVVEAVVAEREKAGPYADFMDFLERVGVRAMNKRAIETLIQAGCFDTMGRGRAEL
                     ALNLERAFDYAQKKAEAGAYGQTSLFEASSEEEFPPFRFEPCQELPRAELLKQEKALL
                     GFYFSGHPLDEYRDVYRKCCDIDFAHIERCAPDKEYTLVGQLVEWREILTRKGKRMAF
                     GKVEGYDGVLEIVVFSATLEANPGRFAQDAIVFLKGTV"
BASE COUNT          203 a          420 c          409 g          260 t
ORIGIN      
        1 cgacggtgcc cttgaggaac acgatcgcgt cctgggcgaa gcggccgggg ttcgcctcga
       61 gtgtagccga gaacacgacg atctccagga cgccgtcgta gccctcgacc ttgccgaagg
      121 ccatgcgctt gcctttgcgc gtcaggatct cccgccactc gaccagctgg ccgaccaggg
      181 tgtactcctt atcgggcgcg cagcgctcga tatgcgcgaa gtctatgtcg cagcactttc
      241 ggtagacgtc ccggtactcg tcgagcgggt gccccgagaa gtagaagccg agcagggcct
      301 tctcctgctt gaggagttcg gcgcgcggca gctcctggca gggttcgaaa cggaacggcg
      361 ggaattcctc ctcgctcgat gcctcgaaca ggctcgtctg accgtaggcg ccggcctcgg
      421 ccttcttctg cgcgtagtcg aaggcgcgct ccaggttgag cgccagctcg gcgcgcccgc
      481 gccccatcgt gtcgaagcag ccggcctgga tcagcgtctc gatagcccgc ttgttcatcg
      541 ccctgacgcc gacccgctcg aggaagtcca tgaaatccgc gtagggaccg gccttctccc
      601 gctccgcgac gacggcttcg acgaccgcct cgccgacgcc cttgacgccg atgagaccgt
      661 agacgatctt gccctcggat accgagaagg tcgcctccga acggttgatg tccggggcca
      721 gaatgccgat gcccatgctc ctggcctcgg agatgtactg ggtcagcttg tcggtgttcg
      781 atatctcgtt cgtcaggttg gcggccatgt actcggccgg ataattggct ttgagccagg
      841 ccgtccggta ggcgaccacc gaatacgcgg ccgcgtggct cttgttgaag ccgtagccgg
      901 caaacggcac caggatgtcg aagatctcct gcgccttctc cttcgtgtag ccgcgcttga
      961 gggcgccctc tatgaatggc gccttctcct tctccaggac ctcgaccttc ttcttgctca
     1021 tcgcgcgccg taggaggtcg gcgcggccga gcgagtagcc ggcgacctcc tgggccacct
     1081 gcatcacctg ttcctggtag acgatgacgc cgtaggtctc cttgagatac ttttcgagtg
     1141 acgggtgcgg gtacgtgatc ggcgtacggc cccatttcga gtcgatgaac tgcggaatat
     1201 tgtccatcgg gccggggcgg tacagggcgt tcagggctat gagatcctcg acgctcgacg
     1261 gcttggcctg cctgagtatg ccctgcatgc cg
//
LOCUS       DAOOLN010000737         1292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_139772_length_1292_cov_2.197251, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000737 DAOOLN010000000
VERSION     DAOOLN010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139772_length_1292_cov_2.197251"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PLW80_09220"
     CDS             <1..293
                     /locus_tag="PLW80_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66733.1"
                     /translation="LQRAQYWVDYRTDDRAVVDYAAMASSVEGVLGVHGGYSVLELRA
                     ALEAAYAHTGLSVVHVPVYAGSDDEGGLGAYGDWNVGAWCERVQREKHRLGW"
     gene            303..>1292
                     /locus_tag="PLW80_09225"
     CDS             303..>1292
                     /locus_tag="PLW80_09225"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPB66734.1"
                     /translation="MDALRVVFAGCGRISDLHVLGYRDEPRAVLHGLYDLDRAAAERR
                     AAEWGAPRVYASYAEVLADPEVDAVEVLVPHPLHEELAVAALRAGKHVSVQKPMTTSL
                     RSADRMLAAARASGRVLKVAENYALYPPIVLARRLIDEGAIGEPMTVRIKMMSGGSGG
                     WPVSDSTWAWRLREFAAGRGMNTFDHGHHMWATAWALLGPFRKVNAWVDEQNGVVDSP
                     AVVMWAHEGPRRYGQCEFHYGKELAVPSSYYGNDEWIDVSGSRGVLVIRRCTASILEG
                     PVVGVYSGGAWAWHEAETDWATGFRLSTKNFIAAMRGDEAPRPSAEEGRHLLAM"
BASE COUNT          186 a          414 c          475 g          217 t
ORIGIN      
        1 cgctacagcg agcgcagtac tgggttgact atcgcaccga cgaccgcgct gtcgtcgact
       61 acgccgcgat ggcctcgtcg gtcgagggcg tcctgggcgt gcatggcggg tactcggttc
      121 tggagctgag ggcggccctg gaagcggcct acgcgcatac cggtctgtcg gtggtccatg
      181 tgcccgtcta cgccgggagc gacgacgagg gtggcctcgg cgcttacggc gattggaacg
      241 tcggcgcctg gtgcgagcga gtgcagcgcg agaagcatcg gctcggatgg taggaggatg
      301 ggatggacgc gttgagggtc gtcttcgccg gctgcggccg tatctccgac ctgcatgtac
      361 tcggttatcg cgacgagccg agggccgttc ttcacggcct ctacgatctc gaccgggcgg
      421 ccgcggagcg tcgggccgcc gagtggggag cgccgcgggt ctatgcctcg tacgccgagg
      481 tcctggccga tcccgaggtc gacgcggtgg aggtgcttgt gccccacccc ctccacgagg
      541 agctggccgt ggccgccctg cgcgccggca agcacgtctc ggtacagaag cccatgacga
      601 cgagcttgcg tagcgccgac cgcatgctcg ccgcggcccg cgcttcgggc cgcgtgctga
      661 aggtggccga gaactatgcc ctgtacccgc cgattgtcct cgctaggcgc ttgatcgacg
      721 agggggccat cggcgagccg atgaccgtcc gtatcaagat gatgtcgggc ggctcaggcg
      781 gctggcccgt ctcggactcg acctgggcct ggcgcctgcg tgaattcgcg gcgggccggg
      841 gcatgaacac cttcgaccac ggtcaccaca tgtgggcgac ggcctgggcc ttgctcggcc
      901 ccttccgtaa ggtgaacgcc tgggtggacg agcagaacgg cgtggtcgac tcccccgccg
      961 tggtcatgtg ggcccacgaa gggcccaggc gctacggcca gtgcgagttc cattacggca
     1021 aggagctcgc ggtgccttcg agctactacg gtaacgatga atggatagac gtctcgggct
     1081 cccggggcgt gctcgtgata cggcgctgca ccgccagcat cctggaaggg ccggtcgtcg
     1141 gcgtctactc gggcggcgcc tgggcctggc acgaggccga gaccgattgg gcgaccgggt
     1201 tcaggctgtc gaccaagaac tttatcgcgg ccatgcgcgg cgatgaggcg cctcgcccgt
     1261 cggccgagga aggccggcac cttctggcca tg
//
LOCUS       DAOOLN010000738         1291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_139939_length_1291_cov_2.237055, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000738 DAOOLN010000000
VERSION     DAOOLN010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139939_length_1291_cov_2.237055"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1291)
                     /locus_tag="PLW80_09230"
                     /pseudo
     CDS             complement(1..1291)
                     /locus_tag="PLW80_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256356.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
BASE COUNT          201 a          383 c          431 g          276 t
ORIGIN      
        1 gatgacctgc cccttgagta ttttctcgcc ggccttgacg atgggccgct gattatagca
       61 ggtgtcctgg ttggtccgct ggtacttgat cagcaggtac tcgtcgcggg ccttggggtc
      121 ggcgtccggc ttgatcacga cgcggagcgc gtcgacccag gccaccacgc cggagcgctt
      181 ggccttgatc aacacgcccg agtcgtaggc gcacttcttc tccatgccgg tgccgacgcg
      241 cggcgggtcg gtaaacagga gcggcaccgc ctggcgctgc atgttcgagc ccataagggc
      301 gcggttggcg tcgtcgtgct cgaggaaggg gatgagcgcc gcggagaccg atatgatctg
      361 cttgggcgac acgtccatgt actggagctc gggcgcggtc ctggtcgtat agtcgccctg
      421 gtgcctgcac gacacctggc cctcgaggaa gttgccgtcc ttgtcgatct tggcgctggc
      481 ctgggcgacg tagtacttgt cctcgtccat cgcggacagg aactcgatgt cgtcggtcac
      541 cttgccgtcc cgcaccttac ggtacggcgt ctcgaggaag ccatactcgt tgacggtggt
      601 atacgtggcg agcgacacga tgaggccgat attcgggcct tccggcgtct cgatcggaca
      661 catgcggcca tagtgcgtgt agtgcacgtc acgtacctcg aagccggctc gatcacggga
      721 caggccgccc gggccgagcg cgttgaggcg ccgcttgtgg gtcagctccg cgagcgggtt
      781 gacctgatcc atgaactggg acagctggct ggagccgaag aactccttga tcgcggcgac
      841 gacgggcttg atcgagatca ggtcctgcgg ccgtatggta tcggtctcct tgagcgacat
      901 gcgctccttg gcgatacgct ccatgcggct gaaggcgctc ttcatcacgt tggccaggag
      961 ctcgccgacc gagcgtacgc ggcggtttcc gaggtggtcg atatcgtcga ggttattctc
     1021 gccgtagtag acggtcatca ggtacttcat cgtcgcgatg atgtcctccg agtcagcgtg
     1081 aagtccttga ccggtaccac gtagtcgaat ttcttattga gcttgtagcg accgaccttg
     1141 cccaggtcgt agcgacggct cgtgaagaac agcgagtgga ggtcgcgctc ggcgttctcc
     1201 accgttatcg gctccccggg catcaaagtc gagtagacgg cggcgagggc gtcggccttg
     1261 gtcggctcgt cctggctcgg gtcttccttg t
//
LOCUS       DAOOLN010000739         1288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_140366_length_1288_cov_3.712895, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000739 DAOOLN010000000
VERSION     DAOOLN010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140366_length_1288_cov_3.712895"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PLW80_09235"
     CDS             <1..470
                     /locus_tag="PLW80_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66735.1"
                     /translation="YKASYDPEKAVREASIAALASIGGQRADDYLSAFLEEPKNGTQY
                     RSVALKAILADGGGEARKKALAVFAAAQSEKDRAPFTAFAKAAMSVDDEAAAPFAELM
                     LGDKDFSMRLGALAWIERNRYAALADAVRSVSETDASDAVKKRAAKALERLSP"
     gene            complement(487..>1288)
                     /locus_tag="PLW80_09240"
     CDS             complement(487..>1288)
                     /locus_tag="PLW80_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66736.1"
                     /translation="SPYATDVGKPAAHASPYATDVGKPAAHASPYATDVGKPAAHASP
                     YATDVGKPAAHASPYAAGVTAPEATHIRDKAALLGAWGGTAASRRALPLASDASREAT
                     AVTAPRGPMSYEPNVVRAGSARLTLRVLGQNPNIGKRNIRAMHSGGRASVGGGSSDFL
                     VFLLPVPRRIAWLYYDGSDATLVPAKPEFFPDYDAPIEACLGRDIRMVTARGKELTLR
                     FERYVSPVERINRLLHCIESPGLSAKPGDDGRSEADAVVALAEPESGV"
BASE COUNT          206 a          420 c          466 g          196 t
ORIGIN      
        1 agtacaaggc ttcctacgac cctgaaaagg cggtgcgcga ggcgtcgatc gccgctctcg
       61 cgagtatagg cggccagcgt gccgacgact acctctcagc gttcctggag gagcctaaga
      121 acgggacgca atatcgatcc gtcgcgctca aggccatctt ggccgacggc ggcggcgaag
      181 cgaggaagaa ggcgctggcc gtgttcgcgg cggcccagtc cgagaaggat agggcgccgt
      241 tcacagcgtt cgccaaggcc gccatgtcgg tggacgacga ggcggcggcg cccttcgccg
      301 agctgatgct cggcgacaag gatttctcga tgcgcctcgg ggcgttggcg tggatcgagc
      361 ggaaccgcta cgccgcgctc gccgatgcgg tacgatccgt ctcggagacg gacgccagcg
      421 acgcggtcaa gaagcgggcg gccaaggcgc tcgagcgcct ctcgccatag ccgatcatag
      481 agatccctac actccggact cgggctcggc cagcgccacg acggcgtcgg cctctgaacg
      541 gccatcgtcg cccggcttgg ctgagaggcc cggcgactcg atgcaatgga gcagcctgtt
      601 gatgcgctcg accggcgaca cgtagcgctc gaacctgagc gtcagttcct tgccacgcgc
      661 ggtgaccatg cgtatatccc tgccgaggca ggcctcgatc ggcgcgtcat agtccgggaa
      721 gaactcgggc ttggcgggta cgagggtggc gtcgctaccg tcgtagtaca gccaggcgat
      781 acgcctgggc accggcaaga gaaacaccag gaagtcgctc gagcctccgc cgaccgacgc
      841 tcgaccgccc gagtgcatcg cgcggatgtt acgcttgccg atgttcgggt tctggccgag
      901 gactctcagg gtcaggcggg ccgagcccgc tcggaccacg ttcggctcat agctcatcgg
      961 gccgcgcgga gccgttacag ccgtcgcctc tctggacgcg tcggaggcca acggcaaggc
     1021 cctgcgcgag gcggcggtac cgccccaggc gccgagtagc gcggccttgt cgcgaatatg
     1081 cgtggcctcg ggcgcggtaa ccccggcggc gtagggcgac gcgtgagccg cgggctttcc
     1141 cacgtcggtg gcgtagggcg acgcgtgagc cgcgggcttt cccacgtcgg tggcgtaggg
     1201 cgacgcgtga gccgcgggct ttcccacgtc ggtggcgtag ggcgacgcgt gagccgcggg
     1261 ctttcccacg tcggtggcgt agggcgac
//
LOCUS       DAOOLN010000740         1288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_140370_length_1288_cov_3.419303, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000740 DAOOLN010000000
VERSION     DAOOLN010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140370_length_1288_cov_3.419303"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1288
                     /locus_tag="PLW80_09245"
     CDS             <1..>1288
                     /locus_tag="PLW80_09245"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF014553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HPB66737.1"
                     /translation="VAAVKARARSGDADQDYAGLGPEELLSAISRRAREIAGKQYAAL
                     TSELLPALAAEGLWLMRPAEYDVESRRWLEDYFMGQVAPALTPLAVPVPEEGSPVAFP
                     TTGNLRIHAAFLLRSPGGEEERLAIVQVPPNLGRFVRLPSGPGARYALLDDLVMDFGH
                     RLFPGYAVTERCLFKVTRDADIGVDEDRDDDFVAAMEEVLVNRQNSWPVRLTISSDSE
                     ELEARLREALGLDQDDVYALPGPIDLKSFMEIANLKGFDRLRYKPRSRFSPLALSEDN
                     TVWDEIRKRDLVLHLPYESFQPIVDFVEAAAADPDVLAIKATLYRTSGDSPVVKALTR
                     AARAGKQVTVVVELKARFDEERNIVWASRLEQAGAIVVFGAARLKVHAKAMLVVRCEE
                     DGLVRRYVHLSTGNYNDKTARLYGDLSLFTANPTICA"
BASE COUNT          202 a          441 c          440 g          205 t
ORIGIN      
        1 gtggcggccg tcaaggccag ggcccgctcc ggcgatgccg accaggacta cgccgggctc
       61 gggcccgagg aactgctgtc ggccatctcg cgcagggccc gcgagatagc cggcaagcag
      121 tacgccgccc tgacgagcga gctgctgccg gccctggccg ccgagggcct ctggctgatg
      181 cggccggccg agtacgatgt cgagtcgagg cgctggctcg aggattactt catgggccag
      241 gtagcgccgg ccctgacgcc gctggccgta ccggtcccgg aggaaggttc gcccgtcgcc
      301 ttcccgacga ccggcaacct gcgcattcac gccgccttcc tgctgcgttc gcccggcggc
      361 gaggaggagc gcctggccat cgtccaggtg cctcccaacc tggggcgctt cgtccggctg
      421 ccttccggtc cgggagctcg ctacgcgttg ctcgacgacc tggtgatgga cttcggccac
      481 cggttatttc cgggctacgc cgtgacggag cgttgcctgt tcaaggtgac cagggacgcc
      541 gacatcggcg tcgatgagga ccgtgacgac gacttcgtcg cggccatgga ggaggtcctc
      601 gtcaaccggc agaattcgtg gccggtcagg ctgaccatct cctccgactc cgaggagctc
      661 gaggcgcggc tccgggaagc gctcggcctg gatcaggacg acgtgtacgc gttgcccggc
      721 cccatcgacc ttaaaagctt catggagatc gccaacctga agggcttcga ccggctgcgc
      781 tacaagccca ggagccggtt ctcgccgctg gccctgtcgg aggacaatac cgtttgggac
      841 gagatacgca agcgagacct ggtgttgcac ctgccctacg agtcgttcca gcccatcgtc
      901 gacttcgtcg aggccgcggc cgccgacccc gacgtgctag cgatcaaggc taccctatac
      961 cggacctccg gcgactcgcc cgtggtcaag gcgctcacgc gggccgcgag ggccggcaag
     1021 caggtcacgg tcgtcgtcga gctgaaggcg cgcttcgacg aggagcgcaa catcgtctgg
     1081 gcgtccaggc tggagcaagc cggcgcgatc gtcgtgttcg gagctgcccg gctcaaggtg
     1141 cacgccaagg cgatgctggt cgtcaggtgc gaggaggacg ggctggtccg gcgctacgtg
     1201 cacctgtcca ccggcaacta caacgacaag accgcccggc tgtacggcga cctgtcgctg
     1261 ttcacggcca acccgaccat ctgcgcgg
//
LOCUS       DAOOLN010000741         1288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_140499_length_1288_cov_1.622871, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000741 DAOOLN010000000
VERSION     DAOOLN010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140499_length_1288_cov_1.622871"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLW80_09250"
     CDS             <1..231
                     /locus_tag="PLW80_09250"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPB66738.1"
                     /translation="PVPEGIEGRVPYKGELRDYLRQLVAGLKKGMGYTGCVDIETLRT
                     YRKFIRITGAGLRESHAHDVAITQEAPNYSRR"
     gene            253..>1288
                     /locus_tag="PLW80_09255"
     CDS             253..>1288
                     /locus_tag="PLW80_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HPB66739.1"
                     /translation="MNVAVIGAQWGDEGKGKIVDYLATDAQIIVRFSGGANAGHTIVR
                     GGEQFALHLVPSGVLYPDKRVILGTGMVIDPEALFAELGMLAERGIDWEGRVFISDRA
                     HRVLPRYRTLDKSLEAARKRPIGTTGRGIGVAYAMKSSRDGVRLADLFDDRLLENLKD
                     DDRRFIAEWRDRLAPLAVDLTHYLSGHRNTYCLFEGAQGALLDLDTGTYPYVSSGMSG
                     AGGAATQGGIGPRRLDKVLGVFKAYSTRVGNGPFPTEFDDGSQSALCQFIRDTGREYG
                     VTTGRPRRCGYLDLVALRYACLANSIDSLVLTHLDVYDTLDSFEACVAYKVRGQLVEH
                     FPASVRDLEDA"
BASE COUNT          218 a          450 c          427 g          193 t
ORIGIN      
        1 cccgtgcccg agggcatcga gggccgggtg ccctacaagg gagagctacg cgactacctg
       61 cgccagctcg tcgcgggcct caagaagggt atgggctata ccggctgcgt ggatatagag
      121 acgctccgca cgtacaggaa gttcatacgg atcaccgggg ccggcctgcg cgagagccac
      181 gcccacgacg tggccataac ccaggaagcc ccgaactact cgcggcgata ggcgatggat
      241 aaggagaacg gcatgaacgt agcggttatt ggcgcccagt ggggcgacga gggcaagggt
      301 aagatagtcg actacctggc gaccgacgcg cagatcatcg tgcgtttctc gggcggcgcc
      361 aacgctggcc ataccatcgt caggggcggc gagcagttcg cgttgcacct cgtcccctcg
      421 ggcgtgctct acccggacaa gcgggtgata ctcggcaccg gcatggtcat cgaccccgag
      481 gccctgttcg ccgagctcgg catgctcgcc gagcgcggca tcgactggga aggccgcgtc
      541 ttcatctcgg accgggcgca ccgcgtgcta ccgcgctacc ggacgctcga caagtccctc
      601 gaggccgccc gcaagcggcc aatcggcacg accggccgcg gcataggcgt cgcgtacgcg
      661 atgaagtcgt cgcgcgacgg ggtcaggctg gccgacctct tcgacgatag gctactcgag
      721 aacctcaagg acgacgatag gcgcttcata gccgagtggc gcgataggct ggcgccgctg
      781 gccgtcgacc tgacgcacta cctctccggc caccggaaca cgtactgcct gttcgagggc
      841 gcgcagggcg ccctcctgga cctggacacg ggcacctacc cctacgtgtc gtccggcatg
      901 tcgggggcgg ggggagccgc cacccagggc ggcatcggcc ccaggcgcct cgacaaggtg
      961 ctcggggtgt tcaaggccta ctcgacccga gtcgggaacg gccccttccc gaccgagttc
     1021 gacgacggca gccagtccgc cctgtgccag ttcatccgcg acaccggccg cgagtacggc
     1081 gtgaccaccg gccgtccgcg tcgttgcggc tacctggacc tcgtcgccct gcgctacgcc
     1141 tgcctggcca actcgatcga ctcgctcgtc ctcacgcacc tggacgtcta cgacacgctc
     1201 gactccttcg aggcctgcgt cgcgtacaag gtgcgcggcc agctcgtcga gcacttcccg
     1261 gcgtcggtcc gcgaccttga ggacgccg
//
LOCUS       DAOOLN010000742         1286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_140787_length_1286_cov_2.398050, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000742 DAOOLN010000000
VERSION     DAOOLN010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140787_length_1286_cov_2.398050"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1286
                     /locus_tag="PLW80_09260"
     CDS             <1..>1286
                     /locus_tag="PLW80_09260"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66740.1"
                     /translation="VRSGVDDRLIAAGKGGFEAGKVADRDVVAERDVVYIDVRATKIR
                     TEAELRLVPAVFSLDDAATRAALAELEAFRLGFASLAGEIEGREELALRLRGRFPDLA
                     ATDLPERLTNYPAPSNAISQGRAVLTALLGAGVFAIPDSGLEQYNPESVELRRWTDGP
                     LVYEQVPLESATTSRRLKAAALDIAKSLKLSATMSALAADLAVAFARENAFFDAEQSL
                     RRLESARARVEPVMRKLAKGDKVIREGFIVTAEDLERLAALRGSEMEFDALYALAGVL
                     LLAALGVASVALLGRGSTGIRVEGASYWFVLAVAVGYFVASSALSAFLPPVLAAHIDA
                     FLPTALVSMLVAILIGERFALMFTLIIGSSLVLVTGLDAFVPAQAIASGLAGIVLVRR
                     ADKRIDLVRAGSQLAAARFAIGFALAALADRGAVAAL"
BASE COUNT          158 a          455 c          459 g          214 t
ORIGIN      
        1 gtgcggagcg gggtcgacga tcggttgatc gcggccggca agggcggctt cgaggcgggc
       61 aaggtggccg accgtgacgt ggtcgccgag cgcgacgtgg tctatatcga cgtcagggcg
      121 accaagatac gcaccgaggc cgagctccgg ctcgtgccgg cggtcttctc gctcgacgac
      181 gccgccaccc gggcggccct ggccgagctg gaggccttca ggctcggctt cgcctccctg
      241 gccggcgaga tagagggccg cgaggagttg gcgctccggc tgcgcggccg cttccccgat
      301 ctggccgcca cggacctgcc cgagcggctg accaactatc cggccccgtc caacgctata
      361 agccagggcc gcgcggtgct gaccgctctc ctcggcgctg gcgtgttcgc gataccggac
      421 tcgggcctcg agcaatacaa tcccgagagc gtcgagctgc gccgctggac cgacggtccg
      481 ctggtttacg agcaggtgcc gctggagtcg gccacgacga gtcgtcggct caaggccgcc
      541 gccctcgata tcgccaagtc gctcaagctg tcggccacga tgtccgcgct cgcggccgac
      601 ctcgccgtcg cgttcgccag ggagaacgcc ttcttcgacg cggagcagag cctccggcgc
      661 ctcgagtcgg cccgagcgcg ggtggagccg gtcatgcgca agctcgccaa gggcgacaag
      721 gtgatacggg agggcttcat cgtcacggcc gaggacctcg agcggctcgc cgccctgcgc
      781 ggctccgaga tggagttcga cgcgctgtac gcgctggctg gcgtgctgct gctcgcggcg
      841 ctcggggtag cgtcggtcgc cctgctcggc aggggctcta ccggcatccg agtcgaaggc
      901 gccagctact ggttcgtcct ggccgtggct gtcggctatt tcgtcgcgtc gagcgccctg
      961 tcggccttcc tgccgcccgt tctcgcggcc catatcgacg ccttcctgcc gacggccctg
     1021 gtgtcgatgc tggtcgccat cctgataggc gaacgcttcg cgctgatgtt cacgctgatc
     1081 atcggctcca gcctcgtgct ggtgaccggc ctcgacgcct tcgtgccggc ccaggcgata
     1141 gcctccggac tggccggtat cgtgctcgtc aggcgcgccg acaagcgcat cgacctggtg
     1201 cgcgcgggct cgcagctggc ggccgcgcgc ttcgccattg gcttcgcgct ggcggcgctg
     1261 gcggaccgcg gcgcggtggc ggccct
//
LOCUS       DAOOLN010000743         1285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_140909_length_1285_cov_3.068293, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000743 DAOOLN010000000
VERSION     DAOOLN010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140909_length_1285_cov_3.068293"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLW80_09265"
     CDS             <1..368
                     /locus_tag="PLW80_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HPB66741.1"
                     /translation="IMAGLACGEPSPIAWDVLRETVDAFVTVPDYVAAKGMRIYATPL
                     KGDPFIVSGESGAVTLGALVTIMQEKGAKALKEALRLDEDSQVLLINSEGNTDPVQFR
                     RIIWEGSNPVPRQHWSERD"
     gene            628..>1285
                     /gene="tyrS"
                     /locus_tag="PLW80_09270"
     CDS             628..>1285
                     /gene="tyrS"
                     /locus_tag="PLW80_09270"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPB66742.1"
                     /translation="MDAKKVAERQLRLLERGCDEIVPREALLEKLERSVETGRPLRVK
                     LGIDPTGNAVHIGHMVPMGKLRQFQDLGHMAVLIIGDYTASIGDPTGRNAERPPLSLE
                     RVRLNAERYAEQIFRVVDGRKAELRWQSEWYGSLSLAELLGLAGSFSLAQLLAHETFR
                     KRYDDGARVGLHELLYPVLQAYDSVMVGADVELDGTDQKFNILAGRELQRDRGLDAQC
                     A"
BASE COUNT          235 a          425 c          429 g          196 t
ORIGIN      
        1 ccatcatggc cggcctggcc tgcggcgagc ccagcccgat agcctgggac gtgctcaggg
       61 agaccgtcga cgccttcgtg accgtgcccg actacgtcgc ggccaagggc atgcgcattt
      121 acgcgacgcc gctcaagggc gacccgttca tcgtttcggg cgagagcggc gcggtgaccc
      181 tcggcgccct cgtcaccatc atgcaggaga agggcgcgaa ggcgctcaag gaggcgctcc
      241 ggctcgacga ggacagccag gtgttgctca tcaattcgga aggcaacacc gaccctgtcc
      301 agttccggcg catcatctgg gagggtagca acccggtgcc caggcagcac tggtcggaac
      361 gggattagac cgggcctagc cgctgaacta gcgccgtgct acagtgacgc catggacgca
      421 atgaacgtta acgctatcta ttattaccgc tcgtactcga cgcgactccc acgagggatc
      481 tcgcgaggct agcggtagtc catacgccgc gatagcccgc ggccggttcc ccgagggggc
      541 gccggccgcg gtttttttat catccgtggc tggcgccccg acgaggccga cccgcgctca
      601 tccgcgacga tccacgagga ggcatacatg gacgcgaaga aggtagctga acgccagctc
      661 aggctgctcg aacggggttg cgacgagatc gtcccccggg aagcgctact cgagaagctc
      721 gagcgtagcg tcgagacagg caggccgcta cgggtcaagc tgggcataga cccgaccggt
      781 aacgccgtgc acatcgggca catggtaccc atgggcaagc tcaggcagtt ccaggacctc
      841 gggcatatgg ccgtgctgat catcggcgac tacaccgcct cgatcggcga cccgaccggg
      901 cgcaatgccg agcggccgcc gctgtcgctg gagcgcgtgc gcctgaacgc cgagcgctac
      961 gctgagcaga tcttccgcgt cgtcgacggg cggaaggcgg agctccgctg gcagagcgag
     1021 tggtacgggt cgctgtcgct cgccgagctc ctgggcctcg ccggatcctt cagcctggcc
     1081 cagctcctag cccatgagac cttccgaaag cgctacgacg atggagcgcg cgtcgggctc
     1141 cacgagctac tctacccggt cctccaggcc tacgactcgg tgatggtcgg ggcagatgtg
     1201 gagctagacg gcaccgacca gaagttcaac atcctcgccg gtagggagct gcagcgggac
     1261 cggggcctcg acgcccagtg cgcga
//
LOCUS       DAOOLN010000744         1285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_140936_length_1285_cov_2.567480, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000744 DAOOLN010000000
VERSION     DAOOLN010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140936_length_1285_cov_2.567480"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PLW80_09275"
     CDS             complement(<1..670)
                     /locus_tag="PLW80_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66743.1"
                     /translation="MIRVKTLPLAIAVVAVMFGGVALSKAVGYWNTTSTKEPAKFKNG
                     ELAGLPNPADIRGSYTWLDLEKAFGVPAAESARAFSTADRALDPAERVSVLEELYLPI
                     LPPGLEIGTGAVRLFVSLYTGLPLEAEEGSVLPDGALELLSQRPGMDDEALGRYVIPS
                     NAPAAPAPAAPAPTAPAPAAPAPAAPAPAAPAGAAPAPAAPAPAAPAPAAPAPAAPAP
                     AAASP"
     gene            complement(667..>1285)
                     /locus_tag="PLW80_09280"
     CDS             complement(667..>1285)
                     /locus_tag="PLW80_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB66744.1"
                     /translation="ALFGPVVCGWICPLGSLQEWLGALGRTLFKKRYNAFIPGRVDKP
                     LRYLRYLSLAWVSYMTIVSGKLVFQDYDPYFALFNFWTGEVAIAGLVILGLTLVLSLF
                     VERPFCKYACPFGAFLGLFNLFRVFSVKRVASTCISCKACDRACPMSITVSTSGTVRD
                     HQCISCLKCTSEDACPVEATVVLAAGKPRVATTTLQATGSEEVSR"
BASE COUNT          234 a          395 c          468 g          188 t
ORIGIN      
        1 tggggcttgc cgccgctgga gcgggagccg ctggagcggg agccgccgga gcgggagccg
       61 ccggagcggg agccgccgga gcgggagccg ctccagcggg agccgccgga gcgggagccg
      121 ctggagcggg agccgctgga gcgggagccg tcggagcggg agccgccgga gcgggagccg
      181 ccggagcgtt ggacggaatg acataacggc cgagcgcctc gtcatccatc cccggccgct
      241 gcgatagtaa ctcgagggcg ccgtccggca gcaccgagcc ttcctccgcc tcgagcggca
      301 ggccggtata gagcgacacg aacagccgta cggctcccgt gccgatctcg aggcctggag
      361 gcagtatcgg caggtatagc tcctcgagca cgctgaccct ctcggccggg tcgagggcgc
      421 ggtcggcggt ggagaaagcc cgagccgact cggcggcggg tacgccaaag gccttctcga
      481 gatccagcca ggtataggag cctcgtatat cggcggggtt cggcaggcca gcgagctcgc
      541 cattcttgaa cttggcgggc tccttggtcg atgtcgtatt ccaatagccg acggccttcg
      601 atagcgcgac gccgccgaac atgacggcga cgaccgctat ggctaacgga agcgtcttga
      661 cgcgtatcat cgcgatacct cctcggagcc ggtcgcctga agcgtcgtgg tcgcgactct
      721 cggcttgccc gccgctagca cgacggtcgc ttcgacgggg caggcgtcct cgctcgtgca
      781 cttgaggcag ctgatgcact gatggtcgcg gacggtaccg ctcgtcgata ccgttatgct
      841 catcgggcag gcgcggtcgc aggctttgca cgagatgcag gtcgacgcga cgcgcttgac
      901 gctaaacact ctgaacaggt tgaacaggcc caggaacgcg ccgaagggac aggcgtactt
      961 gcagaagggc cgttcgacaa acaacgagag gacgagagtc aggccgagga tgacgaggcc
     1021 ggctatggcg acctcgccgg tccagaagtt gaacagggcg aagtacgggt cgtagtcctg
     1081 gaacaccagc ttgccgctca cgatcgtcat gtaggagacc caggccaggg agaggtagcg
     1141 caggtagcga agcggcttgt cgacgcgccc gggtatgaag gcgttgtacc gtttcttgaa
     1201 cagcgtgcgg cccagggcgc cgagccattc ctgcaggctg ccgagggggc atatccagcc
     1261 gcataccacc gggccgaata gcgcg
//
LOCUS       DAOOLN010000745         1282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_141426_length_1282_cov_3.134474, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000745 DAOOLN010000000
VERSION     DAOOLN010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141426_length_1282_cov_3.134474"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="PLW80_09285"
     CDS             complement(<1..1239)
                     /locus_tag="PLW80_09285"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HPB66745.1"
                     /translation="MKRRLSVPQDLRDFAAVFAKAGHSCYFVGGAVRDGLLGRPVSDW
                     DAATDATPERVQALFRSVIPTGVQHGTVTVRWRGASIETTTFRVDGEYRDGRRPESVA
                     FTGDLLADLSRRDFTINGMAVDPATGEVVDPAGGMADLEAGVVRAIGDPIARFTEDGL
                     RPMRAVRFAARFGFSIDPATFAAIPATLCRFRLVSAERVRDEFSKILTCDRPSDGLRS
                     LYDSGLLAEFLPELAACAGVGQGGQHRHDVLGHSIAACQAAPAKLELRLAALLHDVGK
                     PTRRVESADGSLSFHGHELESARLAAAALRRLKYPNAVVDAVSHLVKHHMFDYHDSWT
                     DAAVRRFVSRVGLDWVKPLAILRLADSGGMGAGPADPRSVMPLLERVEALVAKDQAFG
                     LKDLAIGGDELAAAGWPKGPV"
BASE COUNT          205 a          431 c          465 g          181 t
ORIGIN      
        1 gaccgggcct ttcggccagc cggccgccgc cagctcgtcg ccgcctatgg ccaggtcctt
       61 gaggccgaag gcctgatcct tggcgacgag agcttccacc cgctctagga gcggcatcac
      121 cgagcgcggg tccgccgggc ccgcgcccat gccgccggag tcggccagcc tgaggattgc
      181 gagcggcttg acccagtcca gccccacccg cgagacgaag cgcctgacgg ccgcgtcggt
      241 ccacgagtcg tgataatcga acatatggtg cttgacgagg tgcgagaccg cgtcgacgac
      301 cgcgttcgga tacttgagcc gcctcagggc cgccgccgcg agcctggccg actcgagctc
      361 gtgcccgtga aacgataggg agccgtccgc ggactctacc cgtctggtcg gctttccgac
      421 gtcgtgtagc agggccgcga gcctgagctc cagcttggcc ggggcggcct ggcaggcggc
      481 gatcgagtgt ccgagcacgt cgtggcgatg ctggccgcct tgcccgacac cggcgcaggc
      541 ggccagctcc ggtaggaact cggcgaggag gccggagtcg tatagcgatc ggagcccatc
      601 gcttggccgg tcgcaggtca ggatcttcga gaactcatcg cgcacccgct cggctgatac
      661 gagcctgaac cggcaaagcg tcgccggaat ggccgcgaag gtggccgggt cgatcgagaa
      721 gccgaacctg gcggcgaagc gtacggcgcg cataggccgg agaccgtcct cggtgaagcg
      781 ggctataggg tcgccgatgg cgcgaacgac gccggcctcc aggtcggcca tgcctccggc
      841 cgggtcgacg acctcgcccg tggccggatc gacagccatg ccgttgatgg tgaaatcgcg
      901 gcgggacagg tccgcgagca ggtcaccggt gaaggcgacg gactcggggc ggcggccgtc
      961 gcggtactcg ccgtcgacgc ggaaggtagt cgtctcgatc gaagcgccgc gccagcgcac
     1021 cgtgacggtg ccgtgttgga cgccggtcgg tatgaccgac cgaaacaagg cctggacccg
     1081 ctcgggtgtg gcgtcggtgg cggcgtccca gtcgctcacg ggcctaccga ggaggccgtc
     1141 gcgcacggcg ccgccgacga agtagcacga gtggccggct ttcgcgaaga cggcggcgaa
     1201 atcccggagg tcctgaggca cggacagtcg tcgcttcatc gcccgggacc ctaccgtatc
     1261 agcatcgcgt cgccgtacga ga
//
LOCUS       DAOOLN010000746         1280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_141787_length_1280_cov_2.924898, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000746 DAOOLN010000000
VERSION     DAOOLN010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141787_length_1280_cov_2.924898"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..1176
                     /locus_tag="PLW80_09290"
     CDS             154..1176
                     /locus_tag="PLW80_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB66746.1"
                     /translation="MGDTMLEIRNLSAGYATAKGFVRAVDDVSFSLGRGEFLGVAGES
                     GCGKSTLAYAIMRLLRDNARIESGSALFKGRDLAAMTAAEVNAHRWTDMAMVFQSAMS
                     ALNPVLSVGEQLTDAVHAHRSVPRAEAEALAAEMLALVDIHADRLKSYPHQLSGGMRQ
                     RVMIAMALILKPELLIMDEPTTALDVVVQRSIIEKIEELRRRLGFSVIFITHDLSLLV
                     EISDSLGIMYAGRMVEYGPAAELFNDPRHPYTEGLMNSFPSLTGPLRRMDGIEGRTPD
                     LLSPPPGCRFAPRCRYARPTCSAGDPPRLEPLGGGRLSACPYPRRGEAYGCAPAAPAA
                     PEGGSL"
BASE COUNT          187 a          442 c          441 g          210 t
ORIGIN      
        1 tctgggccca ggccaacggc gccctgctgt ccggggcctg gtggtggttc ctgccgcccg
       61 ggatctgcat cgcgctcgtc gggacgtcgt tcgtcctgat caacttcgcg atcgacgaga
      121 tctcgaaccc gcgattacgg aagaggtagg tcgatgggcg atacgatgct ggagatacgg
      181 aacctgagcg cgggctacgc gacggccaag ggcttcgtgc gcgccgtgga cgacgtgtcc
      241 ttctcgctcg ggcggggcga attcctcggg gtggcaggcg agtcgggttg cggcaagtct
      301 acgctggctt acgcgatcat gcgcctcctg cgcgacaacg ctcgcataga gtcgggttcc
      361 gcgctgttca agggccgcga cctggcggcc atgacggcgg ccgaggtgaa cgctcaccgc
      421 tggacggaca tggccatggt gttccagtcg gcgatgagcg cgctcaaccc ggtgctgtcg
      481 gtgggcgagc agctgaccga cgcggtgcac gcgcaccgtt ccgtgcccag ggcggaggcg
      541 gaggccctgg cggcggagat gctcgcgctg gtggacatcc acgccgaccg gctcaagagc
      601 tacccgcacc agctgtccgg cggcatgcgg cagcgcgtga tgatagcgat ggcgctcatc
      661 ctcaagccgg agctgctcat catggacgag ccgactaccg cgctggacgt ggtcgtccag
      721 cgctccatca tcgagaagat cgaggaactc aggcggcgac tgggcttctc cgtcatcttc
      781 atcacccacg acctctcgct gctcgtggag atatcggact ccctgggcat catgtacgcc
      841 ggccgcatgg tcgagtacgg accggccgcc gagctcttca acgatccgcg ccacccgtac
      901 acggagggcc tgatgaactc cttcccgtcg ctgaccggcc cgctacgccg gatggacggt
      961 atcgagggcc ggacgcccga cctgctgtcc ccgccgccgg gctgccgctt cgctccccgt
     1021 tgccgctacg ccaggccgac ctgttcggcc ggggaccccc cgaggctgga gccgctcggc
     1081 ggcggccggc tgagcgcctg cccgtatccg aggcgcggcg aggcgtatgg ctgcgctcca
     1141 gccgctccag ccgctccaga agggggctcg ttatgagcgc cggactgatc cccgcgacga
     1201 acgcggcgcc ggagcgcgcg ccggcgctcg agttgcgctc ggtcgtcaag catttcccgc
     1261 tacgctccct agccgcctac
//
LOCUS       DAOOLN010000747         1279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_141938_length_1279_cov_3.491013, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000747 DAOOLN010000000
VERSION     DAOOLN010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141938_length_1279_cov_3.491013"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="PLW80_09295"
     CDS             complement(<1..1105)
                     /locus_tag="PLW80_09295"
                     /inference="COORDINATES: protein motif:HMM:NF014666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdrA family protein"
                     /protein_id="HPB66747.1"
                     /translation="MKRITVKRGAYYDSVFLMLATRGLKGLAGVRDAIVAMATPMNVE
                     LLCDMGFPSAELDGLGPNDLVVAVDAQNDQAAEAALKEAFDSLTKKRNAEVAGGVAKV
                     GSFAAALAHDPDANVAVISVPGAYAAREARKALQAGLHTMVFSDNVSLDDEIALKKLA
                     VSKGLLMMGPDCGTAIIDGKPLCFANVVRRGPIGVVAASGTGLQETTCLIDRYGSGVS
                     QAIGTGGRDMKNAAVGGMTSLLAIEALSADPSTRAILVVGKPPAGNVAAKVVAALRSS
                     GKPAVAHFLGAPIPADEETIAYASDLEEAALLVTLLADGSNDPASARARIRDDSGNNH
                     YNRPGKAAIETIARAEAERLGASQRYLRGLYTGG"
BASE COUNT          189 a          423 c          438 g          229 t
ORIGIN      
        1 tgccgccggt atagaggccg cgcaggtagc gctggctcgc cccgagccgt tcggcttcgg
       61 cgcgggctat ggtctcgatg gcggccttgc ccggacggtt gtaatgattg ttgcctgaat
      121 cgtcccggat acgggccctg gccgacgctg gatcgttcga tccgtcggct agcagggtca
      181 cgagcagggc ggcctcctcc aggtcggacg cgtaggctat ggtctcctcg tccgccggta
      241 tcggagcgcc gaggaaatga gccacggccg gcttgcccga cgagcgcagc gccgcgacga
      301 ccttggccgc gacgttcccg gccgggggct tgccgacgac caggatcgcg cgggtggacg
      361 ggtcggccga tagcgcctcg atggccagca ggctggtcat gccgcccacg gccgcattct
      421 tcatgtcgcg gccgccggtg cctatggcct ggctgacgcc ggagccgtag cggtctatca
      481 ggcaggtcgt ctcctggaga ccggtgcccg acgcggctac cacgccgatg gggccgcgtc
      541 tgacgacgtt ggcgaagcat agaggcttgc cgtcgatgat cgccgtgccg cagtccgggc
      601 ccatcatcag gagacctttc gataccgcta gcttcttgag cgctatctcg tcatcgagcg
      661 agacgttatc ggagaagacc atcgtatgca ggccggcctg gagcgctttc ctggcttccc
      721 tggcggcgta ggcgcccggt accgatatca ccgcgacgtt ggcgtcgggg tcatgagcga
      781 gggcggccgc gaacgagcct accttggcga cgccgccggc tacctcggcg ttgcgcttct
      841 tcgtcagcga gtcgaaggct tccttgagcg ccgcttcggc cgcctggtcg ttctgtgcgt
      901 cgacagcgac gacgaggtcg ttcggtccga ggccgtccag ctccgccgag gggaagccca
      961 tgtcgcagag gagctcgacg ttcatcggcg tggccatcgc gacgatggcg tcgcgtacgc
     1021 ccgcgaggcc cttgaggccc cgcgtcgcca gcataaggaa cacggagtcg tagtaggcgc
     1081 cgcgcttgac agtaattcgt ttcatcgcat caccgaatcc ttccgttctt atcatagcgc
     1141 atcgctggat ttagggcgat gccttagagc ctctaaaaag ccggccaagg cgtcccggcc
     1201 cgagctggcg cccttgccga gagcggcccg tacggccagg cgcaacccgt cgtcggcccc
     1261 gtcgccggat agaccggcg
//
LOCUS       DAOOLN010000748         1278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_142095_length_1278_cov_4.064595, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000748 DAOOLN010000000
VERSION     DAOOLN010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142095_length_1278_cov_4.064595"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PLW80_09300"
     CDS             complement(<1..925)
                     /locus_tag="PLW80_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPB66748.1"
                     /translation="MIDRNRHGWWREAVFYQIYPRSFMDSNGDGVGDLRGIESRLDYL
                     AWLGVDALWISPFFKSPMRDFGYDISDYEDVDPIFGSLDDFRSLLDAAHRLGIRIVVD
                     LVVNHSSDEHPWFKAARSSRDDPKHDWYIWKPVEGRRPPNNWISLFEQRSAWYPNEAT
                     GEWYLATFTRNQPEFNWRNPELREAIYRVARFWLDLGADGFRMDVATAYFKDAEFRSN
                     PPSPVLVPDLLQRHLYDRNRPEVHGVFREFRALADSYGDRMLIGETHGQDPALAASCH
                     GERNDELHMAFNFDFLFRPWGAESFRRSAERW"
     gene            complement(948..>1278)
                     /locus_tag="PLW80_09305"
     CDS             complement(948..>1278)
                     /locus_tag="PLW80_09305"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPB66749.1"
                     /translation="DEVLKGVAKLIQSQTRSVDVAARYGGEEFCMLLPDTDAATAKLI
                     AERIRRSVEGMGFDYEGQKIAVTLSLGIAQYDQNRDLTGKSVIDRADKALYASKQAGK
                     NRVSVSP"
BASE COUNT          208 a          404 c          430 g          236 t
ORIGIN      
        1 accatcgctc ggccgagcgg cggaacgact cggcgcccca ggggcggaac aggaagtcga
       61 agttgaaggc catgtgcagc tcgtcgttcc gctcgccgtg gcacgaggcg gcgagggcgg
      121 gatcctgccc gtgggtctcg cctatgagca tgcggtcgcc gtatgagtcg gccagcgccc
      181 ggaactccct gaagacgccg tgcacctcgg gccggttgcg gtcgtagagg tggcgctgca
      241 ggaggtcggg cacgagtacg ggactcggcg ggttggagcg gaactcggcg tccttgaagt
      301 acgccgtcgc cacgtccatc ctgaagccgt ccgctccgag gtccagccag aagcgggcga
      361 cccggtagat cgcctcccgt agctcggggt tgcgccaatt gaattcgggt tggttgcgcg
      421 tgaaggtggc caggtaccac tcgccggtag cctcgttcgg gtaccaggcg gaacgctgct
      481 cgaacaggct gatccagttg ttcggcggcc tacggccctc gacgggcttc cagatatacc
      541 agtcatgctt cggatcgtcc cttgaggatc gagccgcctt gaaccacgga tgctcgtcgg
      601 acgaatggtt gacgaccagg tcgacgacta tcctgatacc gaggcggtgg gccgcgtcga
      661 gcagggacct gaagtcgtcc agggagccga agatagggtc gacgtcctcg tagtccgaga
      721 tgtcatagcc gaagtcgcgc atcgggctct tgaagaaggg gctgatccac agcgcgtcga
      781 cgcccagcca ggccaggtag tcgagcctgg actcgatacc gcgcaggtct ccgacgccat
      841 cgccgttcga gtccatgaag ctacgcgggt agatctggta gaagaccgcc tcgcgccacc
      901 agccgtggcg gtttctgtct atcatcgctc tcacctccgg ccgggcgtca gggcgaaacg
      961 gacacccggt tcttgcccgc ctgcttcgac gcgtacagcg ccttgtcggc cctgtctatc
     1021 accgacttgc cggtcaggtc gcggttttgg tcgtactggg ctatgccgag cgacagggtg
     1081 acggctattt tctgcccttc gtagtcgaag cccatgccct cgacgctgcg gcgtatcctc
     1141 tcggcgatca gcttcgcggt ggccgcgtcg gtatcgggca agagcatgca gaattcctcg
     1201 ccgccgtagc gcgcggccac gtcgaccgac cgggtctggc tctggatcag cttggcgacg
     1261 cccttgagca cctcgtcg
//
LOCUS       DAOOLN010000749         1278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_142124_length_1278_cov_3.187244, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000749 DAOOLN010000000
VERSION     DAOOLN010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142124_length_1278_cov_3.187244"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="PLW80_09310"
     CDS             complement(<1..1002)
                     /locus_tag="PLW80_09310"
                     /inference="COORDINATES: protein motif:HMM:NF024768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HPB66750.1"
                     /translation="MRARGFKRGGVPVDHRAPFKTGPIENAFLPLNAIVQLREHRNSV
                     EPVPVVSVGQEVREGQLLARSTGRASSNVHSPIPGIVRRIETTRTPGGFDSKAILVSL
                     EGSFSMLGKRPERYLWKSLNKADIAHIVQDKGIVRVSSGEPLYEILSERSEAPGFVMV
                     VSALEMDPYRRTEEELLALRAEDVVDGCAIAARMALPSRIMFAVDESMDQPTLEPLRE
                     LAARSELDITIQRFRRRFPQDLPGQIAEAMDIADPGTLFIIEPSTLVALHEAVVSNKP
                     HIEQYVYVGGGSIKRPAILKARIGAPIGDLIEECGGFSGHPETIVIGGPFRGREATDL
                     "
BASE COUNT          211 a          425 c          407 g          235 t
ORIGIN      
        1 caggtcggtt gcctccctgc cccggaacgg ccctccgatc acgatggttt ccggatgccc
       61 cgagaaaccg ccgcattcct ctatcaggtc gccgatcggc gcgcctatcc tggccttgag
      121 tatggccggg cgcttgatcg acccgcctcc gacgtagacg tactgctcga tatggggctt
      181 gttcgatacg acggcctcgt gcagcgccac gagcgtgctc ggctcgatga tgaacagcgt
      241 tcccgggtcg gcaatatcca tcgcctcggc tatctgcccg ggcaggtcct gcggaaagcg
      301 acgcctgaag cgctggatcg ttatgtccag ctcgctacgc gcggccagct cccggagcgg
      361 ctcgagtgtc ggctggtcca tagactcgtc gacggcgaac atgatacgcg aaggcagggc
      421 catcctggct gcgatggcgc agccgtcgac cacgtcctcg gccctgagag ccagcagctc
      481 ctcctcggtc cggcggtacg gatccatctc gagcgcgctg acgaccatca cgaagccggg
      541 agcctccgat cgttccgata gtatctcgta gagcggctcg ccactcgata ctctgacgat
      601 acccttgtcc tggactatat gggcgatgtc cgccttgttg agcgacttcc agaggtaacg
      661 ctccgggcgc ttgccgagca tgctgaacga accctcgagc gatacgagga tggccttgga
      721 gtcgaagccg ccgggcgtcc tggtcgtctc gatgcgccgt acgatgcccg ggatgggcga
      781 atgtacgttc gacgacgccc ggcccgtcga acgcgcgagc agctgcccct cgcggacctc
      841 ctggccaacg gacaccacgg gtacaggctc cacggagttg cggtgctcgc ggagctggac
      901 aatagcgttc agaggcaaaa aggcgttctc tatggggcca gtcttgaacg gcgcgcgatg
      961 gtcgactggc acgccgccgc gcttgaatcc gcgagctctc atctatgatc ctactcgttc
     1021 gacacggtcg ggccgaccat ctcgtctgga tgtcaggccc gcgcgagcga gccgggatgc
     1081 gtgcacatta tatagagcaa ccgtaccacc gttgcaaggc cgattcagcg tccgcgggct
     1141 tttttgtcgg agatgggcac gatcgaggat gggacgcgct cgcggagagc cgctagcgcc
     1201 tcggcgtagg ctcctctcga cgccggccgc ggcgccgggt cctcggccgg cgagcgtatc
     1261 atccgcccgc cctcctcg
//
LOCUS       DAOOLN010000750         1277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_142348_length_1277_cov_2.409165, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000750 DAOOLN010000000
VERSION     DAOOLN010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142348_length_1277_cov_2.409165"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..747
                     /locus_tag="PLW80_09315"
     CDS             109..747
                     /locus_tag="PLW80_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB66751.1"
                     /translation="MTNGDDLASASFRQLSEERQNSILSSARTEFATKGFARASTNAI
                     VQACGIAKGSLFNYFDGKDGLFLYLHWLSGKRQLTALRSRSATHGTTQSAMDGLVRAV
                     ELAFDLFADAPDEWSFAMTMAGADAAHLVPRYVALFDAAESAALFRAVVTPPGTDAAV
                     AQALQWMLTGIKMEIQAGLRPASDTESRAETLRGLRSSILGRLEQIRGVIKE"
     gene            751..>1277
                     /locus_tag="PLW80_09320"
     CDS             751..>1277
                     /locus_tag="PLW80_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB66752.1"
                     /translation="MIEVERLCHDYEGKGVLAVDDVSFRAEPGTIYGFLGPSGAGKST
                     VQNILTGLLPLQRGSAALLGQAVTSLRPSFFNRVGYSFELPNLFGALTGYENLAYFAG
                     LFAVPTEDPMRLLERVGLAAAAEKRVSTYSKGMKQRLLFLRAILNRPEVLFLDEPESG
                     LDPTTSLVIKDMIRE"
BASE COUNT          236 a          404 c          422 g          215 t
ORIGIN      
        1 tcaagcggga gacctatccc caggttgcgg aaccagcgcg atcgaacttg accacgtggt
       61 caatatgcgc tatcgtcata tttgaccggg cggtcaagag gagcgaggat gacgaacggc
      121 gacgatctag cgagcgcttc gttcaggcaa ctgtcggaag agcgccagaa ttcgatccta
      181 agctcggcgc gaacggagtt cgcgaccaag ggcttcgcgc gcgcgtcgac caacgccatc
      241 gtacaagcgt gcggcatcgc caaaggctcg ctcttcaact atttcgacgg caaggacggg
      301 ctgttcctat acctgcactg gttgtcgggc aagcggcaac tgacggccct gcgttctcgt
      361 agcgcaaccc acggcacgac gcagagcgcg atggacggcc tcgtgcgagc cgtcgagctg
      421 gcgttcgacc tgttcgccga cgcgccggac gagtggtcct tcgcgatgac gatggcgggc
      481 gcggacgcgg cgcacctggt gccgcgctac gtagcgctgt tcgacgcggc agagagcgcc
      541 gcgttgttca gggccgtcgt tacgccgccg ggcaccgacg ccgcggtcgc gcaggcgctg
      601 cagtggatgc tgaccggcat caagatggag atccaggccg gactacgacc cgcctccgat
      661 accgaatcgc gggccgagac gctccgcgga ctcaggtcgt ccatacttgg acggctggaa
      721 caaatccgcg gcgtcatcaa ggagtagcgc atgatagaag tggagcgcct gtgccatgat
      781 tacgaaggca agggcgtact ggcggtcgac gacgtgagct tccgcgcgga gcccggtacc
      841 atctacggct tcctgggacc gtcgggcgcc ggcaagagca ccgtgcagaa catcctgacg
      901 ggcctcctgc cgctgcagcg cggctccgcg gccctgctcg gccaagcggt gacatcgcta
      961 cggccgtcgt tcttcaaccg ggtgggctac tcgttcgagc tacccaacct gttcggcgcg
     1021 ctaaccggtt acgagaatct ggcgtatttc gccggcctgt tcgcggtacc gaccgaggat
     1081 ccgatgcgcc tgctcgagcg ggtgggtctc gcggcggcgg ccgagaagcg cgtcagcacg
     1141 tattccaagg gcatgaaaca gcggctccta ttcctccgag ccatcctgaa ccgaccggag
     1201 gtgcttttct tggacgagcc cgagtccggg ctggacccga ccacgtcgct cgtcatcaag
     1261 gacatgatac gggagca
//
LOCUS       DAOOLN010000751         1277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_142374_length_1277_cov_2.115385, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000751 DAOOLN010000000
VERSION     DAOOLN010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142374_length_1277_cov_2.115385"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLW80_09325"
     CDS             <1..244
                     /locus_tag="PLW80_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPB66753.1"
                     /translation="SILKKMREPISWFNVHVEADQSDEQPKTYTSMRIIYEFKASDGL
                     KDENVRKAVDLSLERYCGVAALLKKAVPLGSEIAYL"
     gene            445..>1277
                     /locus_tag="PLW80_09330"
     CDS             445..>1277
                     /locus_tag="PLW80_09330"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPB66754.1"
                     /translation="MLRHTFGLFLKHRFRIRAVGSGLFKTVKPPYVIVPTHQGTLDPF
                     MVGYFVPEPVYWVTSDGNMRSSIMKLLLGMVGSIPKSKAIPDMETIGWIMDVIRKRGG
                     VVGIFAEGQASWNGHTQEIIPSTAKLLKLLKVPVIAAVLKGAYFSQPRWSWNKRPGDM
                     EIEFKLVMGGDEAKASSPDRIHEALSEAMEFDEEEWRADHPARHRGNGRAKHLELALF
                     MCPVCGEAGSMRSHVNRLYCRSYGHVVRLSRSYAFTPVGECEPRFRTIREWDAWQARA
                     F"
BASE COUNT          247 a          395 c          414 g          221 t
ORIGIN      
        1 gtcgatcctc aagaagatgc gcgagcccat aagctggttc aacgtgcacg tcgaggccga
       61 ccagtccgac gagcaaccca agacctatac gagcatgagg attatctacg agttcaaggc
      121 ctcggacggt ctcaaggacg agaacgtgcg caaggcggtg gacctctcgc tcgagcgcta
      181 ctgcggcgtg gccgccctgc tcaagaaggc cgtaccgctc ggtagcgaga tagcgtatct
      241 ctaggcccgc caggggcggc ggccgcacgg ccgccgcttt ttaaccggga tatcgtcgtc
      301 ctgaggcgat aggctgacga ttcatccacg atcgcctcga tttctacgtt gcagtatcgg
      361 cgatctcgac tcatagtagt accgaggacg ccatgataga caaagctagg tacaggccgc
      421 gcgccccccc gttggttcaa ccgggtgctg cggcatacat tcgggctctt cctgaagcat
      481 cggttcagga tccgcgccgt cggctcgggg ctgttcaaga ccgtcaagcc gccgtacgtg
      541 atcgtgccga cccatcaggg cacgctcgac ccgttcatgg tcggctattt cgtgccggag
      601 cccgtctatt gggtaacgag cgacggcaat atgcgctcgt cgatcatgaa gctactcctg
      661 ggcatggtcg gatccatccc gaagtccaag gccatccccg atatggagac gataggctgg
      721 atcatggacg tgatccgcaa gcgcggcggc gtcgtcggca tcttcgccga gggccaggcc
      781 agctggaacg ggcatacgca ggagatcatc ccgtcaaccg ccaagctcct caagctgctc
      841 aaggtgccgg tgatcgcggc ggtgctcaag ggcgcctatt tcagccaacc gcgctggtcg
      901 tggaacaagc ggcccggcga catggagata gagttcaagc tggtgatggg cggcgacgag
      961 gccaaggcca gctccccgga ccgcatccac gaggccctgt ccgaggcgat ggagttcgac
     1021 gaggaggaat ggcgggctga ccatccggcg cggcaccgcg gtaacggcag ggcgaagcac
     1081 ctggagctag cgctgttcat gtgcccggtc tgcggcgagg cgggctctat gaggagccac
     1141 gtcaaccgtc tgtactgccg gtcctacggc cacgtcgtca ggctgtccag gagctacgcg
     1201 ttcacgccgg tcggcgaatg cgagccccgc ttcaggacga tacgcgaatg ggacgcctgg
     1261 caggcccggg ccttcga
//
LOCUS       DAOOLN010000752         1275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_142683_length_1275_cov_2.772951, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000752 DAOOLN010000000
VERSION     DAOOLN010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142683_length_1275_cov_2.772951"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..635
                     /locus_tag="PLW80_09335"
     CDS             135..635
                     /locus_tag="PLW80_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HPB66755.1"
                     /translation="MSVSKTTEFGVDQRIVYPSQGVGRIVEIQEKRFKETSVLYYIIY
                     LEFSDMTVMVPVDKARDLGIRPIVPTAEAERALEFIAEEYAPIPSDWKPRYQMNLDLL
                     KKGSVMDIASVVRSLFHRSKIKELPILERKLYDSALNLLQDEVSFSLGKPKDDVAEMI
                     RAKLEG"
     gene            637..>1275
                     /locus_tag="PLW80_09340"
     CDS             637..>1275
                     /locus_tag="PLW80_09340"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HPB66756.1"
                     /translation="MRFAAVIAAAGRSVRFGGAKKEYELLDGLPVLAHSVRLFIEMPD
                     CAAAAVVVPPGGEAEARAALGPALITRAGPRLLFVEGGAERADSVRAGLLALRGADPE
                     YVLIHDAARPWASAELVRRVLAGAARSGACAPGVPVTDTIKRVAADGTIAEHLERAAL
                     RAVQTPQGFLYRGLLGAYLSVGASSRAATDDAEIWAMAGGSVSVVDGDRANVK"
BASE COUNT          208 a          405 c          449 g          213 t
ORIGIN      
        1 gtatgggagc gcgtatggga gcgcgtatgg gagcgcgtat gggagcgcgt atgggcgtgc
       61 atctgtgagc ggccgacgcg gtaggcaggg caggtttgac ttttaggcgg ctgaggagta
      121 gaatcggagt ctgtatgagt gtgtccaaaa cgactgaatt cggcgtagat cagcgtatcg
      181 tctatcctag ccagggcgtc ggcaggatcg tggagatcca ggaaaagcgc ttcaaggaaa
      241 cgagcgtcct ctactatatc atctatctcg agttctccga catgaccgtc atggtaccgg
      301 tggacaaagc gcgcgacttg ggcatccgtc ccatcgtgcc gaccgccgag gcggagcgag
      361 cgctcgagtt catcgccgag gagtatgcgc ccataccgtc cgactggaag ccccggtacc
      421 agatgaacct ggacctgctc aagaagggct ccgtgatgga catcgccagc gtggtgcgtt
      481 cgctcttcca tcgctccaag ataaaggagc tacccatcct cgagcgcaag ctctacgata
      541 gcgccctcaa cctcctgcag gatgaggtct cgttctcgct cggcaagccg aaagacgacg
      601 tcgcggagat gatccgcgcc aagctcgaag gttagcgtgc ggttcgcggc ggtcatcgcg
      661 gccgccggga ggagcgtccg cttcggcggc gccaagaagg agtacgagct cctcgacggt
      721 ctcccggtgc tggcccactc cgtacggtta ttcatcgaga tgcccgactg cgccgccgcg
      781 gccgtcgtgg ttcccccggg cggggaggcc gaggcgaggg cggccctggg gccggcgctg
      841 atcacgcgcg ccgggccgcg gctgctgttc gtcgaaggcg gcgctgaacg cgccgatagc
      901 gtgcgcgccg ggcttctggc cctgcgcggc gccgatccgg agtacgtgct gatccacgac
      961 gccgcgaggc catgggcgag cgccgagctc gttcgccgcg tgctcgccgg agcggcgcgc
     1021 tcgggcgcct gcgctccggg ggtgccggtg accgatacca tcaagcgcgt ggccgccgac
     1081 ggcacgatcg ccgaacacct cgagcgtgcg gccctccgcg ccgtccagac gccgcaaggc
     1141 ttcctctacc gcggcctcct gggcgcctac ctgtcggtcg gggcctcctc gcgggcggcc
     1201 accgacgacg cggagatctg ggcgatggcc ggcggctccg tctcggtcgt cgatggcgat
     1261 cgagcgaacg tgaag
//
LOCUS       DAOOLN010000753         1274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_142829_length_1274_cov_3.182937, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000753 DAOOLN010000000
VERSION     DAOOLN010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142829_length_1274_cov_3.182937"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PLW80_09345"
     CDS             complement(<1..500)
                     /locus_tag="PLW80_09345"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HPB66757.1"
                     /translation="MNDWVFGIDGGGTSSRLRAESMDGAVLYEGSGGSTNLNSNPTEA
                     VTATLEALVSGAFAAGLDPARCRAGFIGSAGVDLDADRETLSGAMRVAFVSAATRAGF
                     AATAVAGARFGAANDAEPALVGALDDVEGFLLIAGTGSIAYGRDRSGRHARAGGLGHF
                     LGDEGSA"
     gene            complement(497..>1274)
                     /locus_tag="PLW80_09350"
     CDS             complement(497..>1274)
                     /locus_tag="PLW80_09350"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HPB66758.1"
                     /translation="ARTGIATVCDFRSGDIAVGGTGAPLVPFFDRIASARFDKPIAFQ
                     NYGGIGNVCWIPENGPLVAFDTGPANMIADRLVSGMTDGRLGYDRDGLIGSRGSLRRD
                     LLARWLDHPFLAKAPPKSSGREEFGSAFYESSLRPLLGGVPEGQNREALYADLIRTAE
                     TFAAASAARAYRELLPALPRLVVVSGGGARNPVIMAELRAALPECEVRDADEVGLSVD
                     YKEAMAFGLFGLLRLLGRPNTEPEATGAARAISAGALYLP"
BASE COUNT          188 a          456 c          452 g          178 t
ORIGIN      
        1 gccgagccct cgtcgccgag gaaatgccca agcccgccgg ccctcgcgtg ccttccgctc
       61 cggtcccggc cgtaggctat cgacccggta ccggctatga gcaggaaacc ttcgacgtcg
      121 tcgagcgcgc cgacgagggc cggctcggcg tcgttggccg cgccgaagcg cgctccggcg
      181 acggccgtcg cggcgaatcc cgccctggtt gcggccgaga cgaaggccac gcgcatcgcg
      241 ccggacagcg tctcgcggtc ggcgtccagg tcgacgccag ccgagccgat gaagcccgcc
      301 cggcagcgcg ccgggtcgag ccccgccgcg aaggcgcccg acacgagtgc ctcgagcgtg
      361 gccgtgaccg cctcggtcgg gttggagttg aggttggtgc tgccgccgga gccctcgtac
      421 aggacggccc cgtccatcga ctcggcgcgc agccgtgaac tcgtcccgcc gccgtcgata
      481 ccgaagaccc agtcgttcat ggtaggtaga gcgcccccgc gctgatggcg cgcgccgcgc
      541 cggtcgcctc cggctccgtg ttcggccgcc ccaggagccg gagcagcccg aacagcccga
      601 aggccatggc ttccttgtag tctacggaca ggccgacctc gtcggcgtct cgaacctcgc
      661 actccggcaa cgccgcgcgc agctccgcca tgatcacggg gttcctggcg ccgccgccgg
      721 agacgacgac gagcctgggg agggctggga gcagctcgcg gtaggccctg gccgcggacg
      781 ccgcggcgaa ggtctcggcg gtacggatga ggtcggcgta cagagcctcg cggttctggc
      841 cttccgggac gccgcccaac aacggccgta ggctcgattc gtagaacgcg gagccgaatt
      901 cctcacgccc ggagctcttg ggaggggcct tggccaggaa ggggtggtcg agccagcgtg
      961 cgagcaggtc gcgtcgcagg cttcccctgg agccgatcag cccgtcgcgg tcgtagccga
     1021 gacggccgtc ggtcatcccg gacacaagcc gatcggcgat catgttggcc ggtccggtat
     1081 cgaaggccac gagcggcccg ttctccggga tccagcagac atttccgatc ccgccgtagt
     1141 tctggaacgc tatcggctta tcgaagcggg cggacgcgat cctgtcgaag aagggcacga
     1201 gcggcgctcc ggtaccgccg acggctatat cgccggacct gaagtcgcag acggtcgcga
     1261 tgccggtgcg cgcc
//
LOCUS       DAOOLN010000754         1270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_143562_length_1270_cov_2.900412, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000754 DAOOLN010000000
VERSION     DAOOLN010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143562_length_1270_cov_2.900412"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /gene="hprK"
                     /locus_tag="PLW80_09355"
     CDS             complement(<1..722)
                     /gene="hprK"
                     /locus_tag="PLW80_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HPB66759.1"
                     /translation="MDEKRFTVLDLLDLDLKEHDALELRCIGGRKGLGRVISAPDINR
                     PGLALSGFFESFAYQRVQLIGHGEAAYLHLLAAEGREEGVSRIFSFPIPCFIFANGEP
                     PAPMFAQAAEAANCPILLSDLPSTELSSRLMRVLTNVFAPRTSVHGVLVEVYGIGILL
                     TGESGVGKSETALELIERGHRLVADDVVEIRCISGNILMGSGANKVIGHHMEIRGLGI
                     INVTHLFGVGAILSLGVLLLRLL"
     gene            complement(871..1161)
                     /gene="raiA"
                     /locus_tag="PLW80_09360"
     CDS             complement(871..1161)
                     /gene="raiA"
                     /locus_tag="PLW80_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPB66760.1"
                     /translation="MTIDVRSVHFDLGEPSRAYLDKKLERIAYAKDLIVDLLFSFTKE
                     KQFKCECTVNFRWGSSAHVQETDFDLAAGMDKLIDTLEQKINKEKSKVQDKK"
BASE COUNT          218 a          397 c          413 g          242 t
ORIGIN      
        1 aggagccgga gcagcaggac gcccagggag aggatggcgc cgacgccgaa gaggtgggtt
       61 acgttgatga tgccgaggcc gcggatctcc atatggtggc cgatgacctt gttggccccc
      121 gaacccatca ggatgttgcc ggatatgcag cgtatctcga cgacgtcgtc ggcgacgagg
      181 cgatggccgc gctctatcag ctcgagcgcg gtctcgctct tgccgacgcc cgactcgccg
      241 gtcagcagga tgccgatgcc gtacacctcg acgagcacgc cgtgcacgct cgttctcggc
      301 gcgaatacgt tggtcagcac gcgcatgagc ctggacgaca gctccgtcga gggcaggtcc
      361 gatagcagga tggggcagtt ggcggcctcg gccgcctggg cgaacatcgg ggccggcggt
      421 tcgccgttgg cgaagatgaa gcacggtatc gggaacgaga agatcctcga gacgccttcc
      481 tcgcgaccct cggcggcgag caggtgcagg tacgccgcct cgccgtggcc tatcagctga
      541 acgcgctggt aggcgaagct ttcgaagaag ccggacaggg ctagccccgg ccggttgatg
      601 tccggggcgc tgatgaccct gcccaggccc ttgcggcccc cgatgcagcg caactcgagc
      661 gcgtcgtgct ccttgaggtc gaggtcgagc aagtcgagta ccgtaaagcg tttctcatcc
      721 atcgcccgga gcatactccg tttcccgggc ttgcgcaacg atattaactg aggcggctcg
      781 acgcgatagc ctgccccgga gccgtcgccc gagacggcga aggccccgcg gcgcgagcgg
      841 cggggccttc gatcgagcgg ctcgtggagc ttatttctta tcctggacct tcgacttttc
      901 cttgttgatt ttctgttcga gggtatcaat cagcttgtcc atccccgcgg ccaggtcgaa
      961 gtccgtctcc tgcacgtggg cgctcgagcc ccagcggaag ttgacggtgc actcgcactt
     1021 gaactgcttc tccttggtga acgagaacaa gaggtcgacg atcagatcct tcgcgtacgc
     1081 gatgcgctcc agcttcttgt ccaggtaagc cctgctcggt tcgccgaggt cgaagtgcac
     1141 ggaacggacg tcaatagtca tgcaagcctc cttgtgccgg tgagaccggc tgattgcgcc
     1201 tagccgcgct tgcggccgaa cgatgaatcc atgttcaact cgcttcggta cttggccact
     1261 gtcctcctcg
//
LOCUS       DAOOLN010000755         1270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_143621_length_1270_cov_2.167901, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000755 DAOOLN010000000
VERSION     DAOOLN010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143621_length_1270_cov_2.167901"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PLW80_09365"
     CDS             <1..523
                     /locus_tag="PLW80_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB66761.1"
                     /translation="KLLAFDAGWPCSLLEYARGVKAAGYRFDCIRWAIISHFHLDHAG
                     LVRDFQDAGIQCLLFENQGDGIEGMERLIARSHKDYRPIFRDRFLPLRTDESRAFLSS
                     IGIAGEVIRTPGHSDDSVSLVTDAHEALIGDLYPLSQVMDDDEASLGSWAAIRALGAR
                     HIFPSHAEPFELA"
     gene            625..1125
                     /locus_tag="PLW80_09370"
     CDS             625..1125
                     /locus_tag="PLW80_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HPB66762.1"
                     /translation="MRNTALVVIDIQNDITKHYEDVIGNINAAIDWAVDSGIPVVYIR
                     HENLSEGTRTFKPNTRGAELVPDMNVVSDNICTKSKGNALTSEAFASFIAANGIGEFY
                     LAGADAVACVKSTCYNMRKTGYGVTVLSDCITSYDTTKIDEMLRYYEAQGSRIMRLAD
                     VVASRR"
BASE COUNT          273 a          379 c          384 g          234 t
ORIGIN      
        1 gaagctactc gcgttcgacg ccggctggcc ttgttcgttg ctcgagtacg ccaggggcgt
       61 gaaggcggcc gggtacagat tcgactgcat taggtgggct ataatctcgc atttccacct
      121 cgaccacgcc ggcctggtcc gggacttcca ggacgccggc atacagtgcc tgctgttcga
      181 gaaccagggc gacggcatcg aaggcatgga gcggctcatc gcgcgtagcc ataaggacta
      241 ccggccgata ttcagggacc ggttcctccc tctcaggacc gacgaatcga gggcgttcct
      301 gtcgtcgatc ggtatcgccg gcgaggtcat caggacgccg gggcattcgg acgacagcgt
      361 gtcgctcgtc accgacgccc acgaggccct gataggcgac ttgtatccgt tatctcaggt
      421 catggacgac gacgaggcga gcctaggatc gtgggccgcc atacgggcgc tcggcgcgcg
      481 gcatatcttc cctagccacg ccgagccgtt cgagctggcg taggcgaata aaacgaaatc
      541 attcactatc actgacatct attgtcaacg atgccgggta tactgacagg gtaagcgaac
      601 cgaacgacta tcaggaggga agccttgcgg aacacagcct tggtcgtaat cgacatccag
      661 aacgacataa ccaagcatta cgaggacgtc atcggcaaca tcaacgcggc catcgattgg
      721 gcggtcgata gcggcatccc cgtcgtctac atacggcacg agaacctctc ggaaggtacc
      781 cggacgttta agccgaatac ccgcggcgcg gaattggtcc ctgacatgaa cgtcgtatcc
      841 gacaacattt gtaccaagtc caaggggaac gcgctgacga gcgaggcgtt cgcctccttt
      901 atcgccgcca acgggatagg tgagttctac ctggcgggag ccgacgcggt cgcctgcgtc
      961 aaatcgacct gctacaatat gcgcaagacc ggctacggag tcaccgtcct atcggattgc
     1021 ataacgagct acgatacgac gaagatcgac gagatgctgc gctactacga ggcacagggc
     1081 agcaggatca tgcgactagc ggacgtggta gcgagccgtc gttagccttc tcactcgtgg
     1141 acgtaccgac ccgtagttat gcggcgagcg aagcgcgtcc ggcgttcgtt tcgctcgccg
     1201 cgtttcggca tggatgctga aaccctactc gcaaacagac acgacgcgtc gtgtgacgcc
     1261 cgcggcgcgt
//
LOCUS       DAOOLN010000756         1269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_143729_length_1269_cov_2.719934, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000756 DAOOLN010000000
VERSION     DAOOLN010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143729_length_1269_cov_2.719934"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="PLW80_09375"
     CDS             complement(<1..875)
                     /locus_tag="PLW80_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66763.1"
                     /translation="MIDENRLGHRSLPILAGALSSVFYGSVALMPIFLVPIQYTGARR
                     GFKAMVGSAAVSSIVIAAWQTIALARADILSAGTVAVGVSTPLALAIALVAMAWPRLV
                     RFSFTIRALVAAAVASALSLPALFAAIRDPAVRAMFLDAFETASSALGAEVLDAEALW
                     SALRTGVASSYGAVLFAFLFLSSWSGSSLGRAMDARTGGRDDAPESLTIQVPNLAAYR
                     VPRGLVWALLAAWTALLVNRFVSVPVFSAVALNAALALSICYGVQCLAVVRALAERVG
                     LAPALRLIGPLAIILL"
     gene            complement(881..1186)
                     /gene="rpsR"
                     /locus_tag="PLW80_09380"
     CDS             complement(881..1186)
                     /gene="rpsR"
                     /locus_tag="PLW80_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPB66764.1"
                     /translation="MSDMQNRDDRGSRNDEGRGDERGDRGGRGGRKGFFRKKVCKFCS
                     QKLKIDYKDPETLKRFITERGKILPRRITGSCAKHQRSLAVAIKRDRALAYIPYVTK"
BASE COUNT          249 a          404 c          420 g          196 t
ORIGIN      
        1 aggaggataa tcgccaaggg gccgatgagc cgcaaggcag gggccagccc gacgcgttcg
       61 gctagcgcgc ggacgacggc gagacactgg acgccgtagc agatcgataa tgctaaggcc
      121 gcgttcaacg cgaccgccga aaacacggga actgaaacaa aacggttgac gagcaacgcc
      181 gtccaggccg ctagcagcgc ccatacgaga ccgcgaggaa cgcgataggc ggccaggttc
      241 ggcacctgaa tcgtcagcga ctctggcgca tcgtcacggc ctccggtccg cgcgtccatg
      301 gcgcggccga ggctcgagcc agaccacgag ctcaggaata ggaacgcgaa cagcacggct
      361 ccgtaactgg acgccactcc cgtccggagg gccgaccata gcgcctcggc gtccaggacc
      421 tccgcgccga gggccgagct ggccgtctcg aacgcgtcga ggaacatggc tcgcacggcc
      481 ggatcccgta tcgcggcgaa cagggccggc aggctcagtg ccgaggcgac tgccgcggcc
      541 acgagggcgc ggatcgtgaa cgagaaacgc acgagcctgg gccatgccat cgcgacgagg
      601 gcgatcgcca gggccagggg cgtcgagacg ccgacagcta cggtcccggc gctcaggata
      661 tcggccctgg ccaaagctat cgtctgccag gcggcgatta cgatcgacga gaccgcggcg
      721 ctccctacca tggctttgaa gccacgcctg gctcccgtgt attgaatggg aaccaggaag
      781 atcggcatca aggccacgga gccgtagaac accgacgata gcgctcccgc caagatgggg
      841 agcgagcggt gcccgaggcg attctcgtcg atcaaggcga ttacttcgtc acgtacggga
      901 tatacgccag ggcgcggtcg cgcttgatgg ccacagccag actacgctgg tgcttggcgc
      961 aggagccggt tatcctacgg ggcagtatct tgccgcgctc ggtgatgaaa cgcttgagcg
     1021 tctcaggatc cttatagtcg atcttgagct tctgcgagca gaatttacag accttcttgc
     1081 ggaagaagcc tttgcgtccg ccgcggccgc cgcggtcgcc gcgctcgtcg cccctgccct
     1141 cgtcgttgcg ggagccgcga tcgtccctgt tctgcatatc tgacatagat gtgtctcctt
     1201 cgtatgtgtc ccgttctcgc tagaacggta tgtcgtccga gaaatcgtcg gacgccggag
     1261 cctgggcgg
//
LOCUS       DAOOLN010000757         1269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_143793_length_1269_cov_2.087315, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000757 DAOOLN010000000
VERSION     DAOOLN010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143793_length_1269_cov_2.087315"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="PLW80_09385"
     CDS             <1..862
                     /locus_tag="PLW80_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66765.1"
                     /translation="EKNKTAITKAESEKRLAALEKEFSAWTDFANSAASSIGDVFSNL
                     TEMAQMELDAQDKAWNDSWDARYGKMEESMKRVAEIYGTDSMEYRQALKEKEDADKLK
                     ADHDALMEKRRKDADNKYKKGSIATDMVKATAEFAKASFGIWARETSTKGFKGIPIAI
                     ALQAMLAASFGTQMGVMAKQMSLVGKMRLGGPVFGPSHESGGVDRNLEGGEFVINAQS
                     MKVPGVAQLANSLNRTGQGGSAPVVSRIDKEDISEIVGAIAAIPVVVSERDITGTQRR
                     VAVIESKTSF"
     gene            880..>1269
                     /locus_tag="PLW80_09390"
     CDS             880..>1269
                     /locus_tag="PLW80_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66766.1"
                     /translation="MPKPKPNETQDEFISRCIPMVINEGTTDDPAQAAAICHSKWREG
                     FSAPQYSSALPLYECAGTEDGAGVKLISLVSRPAIGVGFMMFSAEERSTPLAFFHDEE
                     MLVTGPAIIPNLRIYRRDDKRGEYDVFF"
BASE COUNT          300 a          359 c          419 g          191 t
ORIGIN      
        1 cgagaagaac aagacggcca taacgaaggc cgagtccgag aagaggctgg ccgcgctcga
       61 gaaggagttt tccgcgtgga ccgacttcgc aaactccgca gcgtctagta ttggcgacgt
      121 attctccaac ctcaccgaga tggcgcagat ggagctggac gctcaggaca aggcgtggaa
      181 cgacagctgg gacgcgaggt acggcaagat ggaagagtct atgaagcgcg tcgccgagat
      241 atacggcacc gacagtatgg agtacaggca ggcacttaag gagaaggagg acgcagacaa
      301 gctcaaggcc gaccacgacg cgcttatgga gaagaggcgc aaggacgccg acaacaagta
      361 caagaaggga tccatcgcca ccgatatggt caaggccacc gcggagttcg ccaaggcgag
      421 cttcggcata tgggcgaggg aaacatcgac caagggcttt aagggaatac cgatagccat
      481 agcgctgcag gcgatgctcg ccgcgtcgtt cggcacgcag atgggcgtta tggcgaagca
      541 gatgtcgctc gtgggcaaga tgcgcctcgg cggcccggtc ttcgggccga gccacgagtc
      601 cggcggcgtc gacaggaacc tcgagggcgg cgagttcgta atcaacgcgc agtcgatgaa
      661 ggtcccgggc gtcgcgcagc tcgcgaactc tctcaaccga acagggcagg gaggttcagc
      721 gccggtcgtg tcgaggatag acaaggagga tatttcagag atagtgggcg cgatagcggc
      781 cataccggtg gtcgtgtcgg agcgcgacat caccgggacg cagcgccgcg tggcggttat
      841 agagtccaag acgagctttt aaaaaacaac attacggata tgccaaaacc gaaacccaac
      901 gaaacgcagg acgagttcat ctcgcgctgc atacctatgg tcataaacga gggtaccacc
      961 gacgacccgg cgcaggccgc ggccatatgc cactcgaagt ggcgcgaggg cttctccgcc
     1021 ccgcagtact cgagcgcgct gccgctgtac gaatgcgcgg gcaccgagga cggcgcgggc
     1081 gtgaagctca tatcgctcgt gtcgcgcccc gcgataggcg tgggctttat gatgttctcc
     1141 gccgaggagc gctccacccc gctcgcgttc ttccacgacg aggagatgct cgtgacgggc
     1201 cccgccatca taccgaacct gaggatatac aggcgcgacg acaagcgcgg cgagtacgac
     1261 gtgttcttc
//
LOCUS       DAOOLN010000758         1267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_144092_length_1267_cov_3.151815, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000758 DAOOLN010000000
VERSION     DAOOLN010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144092_length_1267_cov_3.151815"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PLW80_09395"
     CDS             <1..571
                     /locus_tag="PLW80_09395"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPB66767.1"
                     /translation="DRTTDGSGRVRDMSLAQLRELDASASFKGAYGRNRIPTLDEYLD
                     LVAGAGIVTNIELKNGVYRYPGMEERLVATIRRRGLERRTIFSSFNHQSAAICAALCP
                     EAEVAFIESAWLLGAGAYCEARGARYLNPRHTFLTPENAAELAAHSVRAQAWTVDDPV
                     ELRRLAALGIDSIISNDPAAAMEALAALA"
     gene            complement(589..>1267)
                     /locus_tag="PLW80_09400"
     CDS             complement(589..>1267)
                     /locus_tag="PLW80_09400"
                     /inference="COORDINATES: protein motif:HMM:NF018766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HPB66768.1"
                     /translation="GRLAVVAGLGFLSCSLFVAVRHWLTGRFAQGYLLWNLLLAAAPV
                     VIAWAGLRLSYGGRRSRAAALAAFVAWLIFYPNAPYIFTDFIHVVRRAGLGSVAAPWL
                     SEYDLLWYDIVMNAAFAFVGHYLGLVSMYLMHGAARRLFGRAAGWAAMAPAILLSGLG
                     IHLGRFSRFNSWDLLIHPVQAVRVIRESIADPAALLFSTAFSLFIALTYLVFYVVKRG
                     DIGELDG"
BASE COUNT          249 a          427 c          437 g          154 t
ORIGIN      
        1 cgaccgtacg accgacggct ccgggcgggt acgcgacatg agcctcgccc agctgcgcga
       61 gctcgacgcg agcgcctcgt tcaagggcgc gtatggcagg aaccgtatcc ctaccctcga
      121 cgagtacctc gacctggtgg ccggggcggg tatagtcacc aatatcgaac ttaagaacgg
      181 cgtctaccgc tatccaggca tggaggagcg gctggtcgcg acgatacggc gccgtgggct
      241 cgagaggcga acgatcttct cgtccttcaa ccaccagtcc gcggccatct gcgccgcact
      301 ctgtcccgag gccgaggtag ccttcatcga gtcggcctgg ctactcggcg ccggggcgta
      361 ctgcgaggca cgaggagcaa gatacctgaa cccgcgccac accttcctaa cgcccgagaa
      421 cgcggccgag ctggccgcgc atagcgtgcg cgcccaggcc tggacggtcg acgacccggt
      481 ggagctacgc cggctggcag cactcggtat cgactcgatc atatcgaacg acccggcggc
      541 cgcgatggaa gccctggccg ccctggcctg accccgggga ccgagcccct agccgtcgag
      601 ctcgcctatg tcgcctcgct tgacaacgta gaagaccagg tacgtcagcg ctatgaacag
      661 cgagaaggcc gtggagaaca ggagcgcggc cggatcggct atcgactcgc ggatgacgcg
      721 gacggcctgg acaggatgga tcaagagatc ccagctgttg aagcgcgaga agcggcccag
      781 gtggatgccc aagcccgaca ggaggatggc gggagccatc gcggcccagc cggcggctcg
      841 cccgaagagc cgccgcgccg ccccgtgcat caggtacatc gaaacgagcc ccaggtagtg
      901 cccgacgaac gcgaaggccg cgttcatcac gatgtcgtac cagagcaggt cgtactccga
      961 cagccagggt gccgcgaccg agccgagccc ggcccgccgg acgacatgga tgaagtcggt
     1021 gaagatgtac ggcgcgttcg ggtagaagat cagccacgcg acgaaggctg ccagggccgc
     1081 ggcccgggag cgccggccgc cgtaggatag gcggaggccg gcccaggcta tgacgaccgg
     1141 cgcggccgcc aggaggaggt tccatagcag gtagccctgc gcgaaccggc cggtcagcca
     1201 gtggcggacc gccacgaaca gcgaacagga caggaagccg agcccggcca cgacggccag
     1261 ccgcccg
//
LOCUS       DAOOLN010000759         1266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_144287_length_1266_cov_2.791082, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000759 DAOOLN010000000
VERSION     DAOOLN010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144287_length_1266_cov_2.791082"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            310..>1266
                     /locus_tag="PLW80_09405"
     CDS             310..>1266
                     /locus_tag="PLW80_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPB66769.1"
                     /translation="MSDFWTQMPEMAARLDAVRARMRLVVSGARFPLSHAVVSLIDAN
                     GKMLRPAFMLLAGGFGRRRNVDLTGLAAAIELLHIATLIHDDVIDASPTRRGAPTLHT
                     AYGAKDAVLAGDWLFSRCFALAAQSSSPGNATLLASLIGALCSEEIHQDLERFAWPTS
                     ERNYLRKIAGKTAALFSLSLRAGAVEAGASPSVVATLTRVGYDVGMAFQIMDDVLDYE
                     STEGELRKPVGNDVREGLCTLPLILALEAGAPGLRPLLGPAAPNDDSVAAIVAAVRDS
                     GALSSAKARAATYTDRAMRELASLPRGRAREDLHAIVGRLLTR"
BASE COUNT          234 a          427 c          388 g          217 t
ORIGIN      
        1 cgactactgg tccgtgccct tcttcaagaa gtggtggaac ggcaccaagt tcgcccgcag
       61 atggcacttc tacgccgacg acccgacgaa gtaagcgatt ctggaactga tggagcgtta
      121 ggcctttagc tatagaggca cggagacaca gagggagacg tagcaagcga agccaaggag
      181 gaagcatggg atatgatcat ctctcctcca tcgcttcact actttctctc ttctccgtgt
      241 ctcctcgccc cactggtatc ctacttgagt cctttgcata gagcaccgga gaagcgagcc
      301 gtatagacga tgtcagattt ctggacccag atgcccgaga tggcggcaag gctcgacgcc
      361 gtgcgcgccc ggatgcggct tgtcgtctcc ggagccaggt tcccgctgtc gcatgccgtc
      421 gtcagcctga tcgacgcgaa cggtaaaatg ctcagacccg ccttcatgct gctggccgga
      481 ggattcgggc gccgcaggaa cgtcgacctg accggcctcg cggccgcgat cgagctactg
      541 catatagcca cactgatcca tgacgacgtg atcgacgcct cgcccacccg acgaggcgcc
      601 ccgacgctcc ataccgccta cggcgccaag gacgcagttc tggccggcga ctggctgttc
      661 tcccgatgct tcgcgctggc cgcacagagc tccagcccgg gcaacgctac gctactcgcc
      721 tcgctgatcg gcgcgctatg ctcggaggag attcatcagg acctcgagcg attcgcctgg
      781 ccgacgagcg aacgcaacta cctgcggaag atagccggca agacggcggc cttgttctcg
      841 ctgtcgctgc gcgccggagc cgtcgaggcc ggcgcgtcac cgtccgtcgt cgcgacgctc
      901 acccgcgtcg gctacgacgt cggcatggcg ttccagatca tggacgacgt gctggactac
      961 gagtcgaccg agggcgagct gcgtaagccg gtaggcaacg acgtacgcga gggcctgtgt
     1021 accctgccac tgatcctcgc actcgaggca ggcgcacccg gcctgcggcc attgctcggc
     1081 ccggccgccc cgaacgacga ctcggtagcg gccatcgtcg cggcggtcag ggactcgggc
     1141 gcgctcagct cggccaaggc cagggccgcc acctacaccg acagggccat gcgagaactc
     1201 gcgagccttc ctaggggtag agcccgcgaa gaccttcatg ctatcgtcgg acgcctactg
     1261 acccgt
//
LOCUS       DAOOLN010000760         1265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_144420_length_1265_cov_4.002479, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000760 DAOOLN010000000
VERSION     DAOOLN010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144420_length_1265_cov_4.002479"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1265
                     /locus_tag="PLW80_09410"
     CDS             <1..>1265
                     /locus_tag="PLW80_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPB66770.1"
                     /translation="VADGIGDVDKGFAEADVVIERTYVAKRVQCTPMEPHVVYAKVDA
                     DRLVIHASTQVPWHVRRIVATAIGIPEHKVRIIKERVGGGFGAKQDIVLEDVAGYLAW
                     RTRRPVLFRYDRAMEFMTSRTRHGMEVTVKLGAKRDGTLTAMDMRVEADTGPYGQHCL
                     TVPMNAVSKSLPLLLCDNARFSVRTYYTNLSPAGAYQGYGAPKGSFALQGTLAELAAE
                     LGMDQLALIEKNMVRSGSRLEILKSLGEGRPGAVVTLGECGLDRMTALGRKAFNWDAP
                     KPTSDDPDWTIGRGAVIIQQGSGLPGIDAANAEIRLLPDGSLLMLSGGADLGTGLDTV
                     TAKAAAEILCVDMGDVTVVSGDTDTTPFDSGAYASSGTFFSGNAAVKAAEDLRDKCLA
                     LAANLLGEPVENLELVHRGVVRGKKGEIT"
BASE COUNT          215 a          434 c          431 g          185 t
ORIGIN      
        1 cgtggccgac ggtatcggag acgtcgataa gggcttcgcc gaggcggatg tcgtcatcga
       61 gcggacctac gtggccaagc gcgtgcagtg tacgccgatg gagccccacg tggtctacgc
      121 caaggtcgat gccgaccgcc tcgtgatcca cgcctcgacg caggtgccct ggcacgtcag
      181 gcgcatcgtc gccacggcca tcggtatacc tgagcacaag gtccgtatca tcaaggagcg
      241 cgtcggcggc ggtttcggcg ccaaacagga catcgtgctc gaggacgtgg ccggttacct
      301 ggcctggcgc acgcgtcggc cggtgctgtt ccgctacgac cgcgccatgg agttcatgac
      361 gagccgcacc cggcacggca tggaggtcac cgtcaagctc ggcgccaagc gcgacggcac
      421 gttgacggcg atggacatgc gggtcgaggc cgacaccggc ccctacggcc agcactgcct
      481 gaccgtgccg atgaacgccg tgtccaagag cctgccgctc ctgctctgcg acaacgcccg
      541 cttctcggtc cgcacctact atacgaacct gtcgcctgcc ggcgcctacc agggctacgg
      601 cgcgccgaag ggctcgttcg cgctgcaggg cacgctggcg gaactggccg cagagctggg
      661 catggaccag ctggcgctga tcgagaagaa catggtccgc tcgggctcca ggctcgagat
      721 actcaagagc ctcggcgagg gacgccccgg cgccgtggtg acgcttggcg aatgtggact
      781 ggaccggatg acggcgctcg gccggaaggc ctttaactgg gacgcgccga aacccacctc
      841 ggacgacccc gactggacga taggccgagg cgccgtgatc atccagcagg gctcgggcct
      901 gcctggcatc gacgcggcca acgcggagat acggctcctg cccgacggct ccctcctgat
      961 gctgtccggg ggcgccgacc tcggcacggg tctcgatacg gtgacggcca aggccgcggc
     1021 cgagatcctc tgcgtcgaca tgggcgacgt gaccgtcgtc tcgggcgaca ccgataccac
     1081 gccgttcgat tcgggcgcct acgccagctc aggcaccttc ttctccggca acgccgcggt
     1141 caaggccgcg gaggacctca gggacaagtg cctggccctc gccgcgaacc tgctcggaga
     1201 gccggtcgag aacctggagc tggtgcaccg cggcgtcgta cgcggcaaga agggcgagat
     1261 caccc
//
LOCUS       DAOOLN010000761         1262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_144955_length_1262_cov_3.550124, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000761 DAOOLN010000000
VERSION     DAOOLN010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144955_length_1262_cov_3.550124"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /gene="pyk"
                     /locus_tag="PLW80_09415"
     CDS             complement(<1..908)
                     /gene="pyk"
                     /locus_tag="PLW80_09415"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPB66771.1"
                     /translation="MNTQKLTKIVATISDSQCEPEFLRSLYEAGMNVVRLNTAHQTPE
                     ASAKVVANIRKVSDTIGILVDTKGPEVRVKGLPAPLELKAGETVTIPKVDEVRKGFSV
                     SYAGFTAEVPVGSRILIDDGAIELTVKNVANGMLLCEVMNDGLLKDRKSVNAPGVPLR
                     IPALSDKDREYLKFVADNDVDFIAHSFVRNKDDVLEVRRILDGYGSKARIIAKIENIQ
                     GVTNLPEILDFADGAMVARGDLGVEIPFERLPGIQKTIIDACVRKGKPVITATQMLHT
                     MIDNPRPTRAEVSDVANAVFDGTDAL"
BASE COUNT          197 a          403 c          417 g          245 t
ORIGIN      
        1 atcagcgcgt cggtgccgtc gaacacggcg ttggccacgt cggagacctc ggcgcgcgtc
       61 ggccgcgggt tgtctatcat cgtgtggagc atctgggtgg cggtgatcac cggcttgccc
      121 ttgcgcacgc aggcgtcgat gatggtcttc tggatgcccg gcaggcgctc gaagggtatc
      181 tccactccca ggtcgccgcg cgcgaccatc gcgccgtcgg cgaagtcgag tatctcgggc
      241 aggttggtca cgccctggat gttctcgatc ttggcgatga tcctggcctt gctgccgtag
      301 ccgtccagga tgcgcctgac ctcgagcacg tcgtccttgt tgcgcacgaa cgagtgggcg
      361 atgaagtcga cgtcgttgtc ggctacgaat ttgaggtact cgcggtcctt gtccgacagg
      421 gccggtatcc gcagcggcac gcccggcgcg ttgacgctct tgcggtcctt gaggaggccg
      481 tcgttcatca cctcgcagag cagcatcccg ttcgcgacgt tcttgaccgt cagctcgatc
      541 gccccgtcgt cgatcaggat gcgcgagccg accggcacct ccgcggtgaa gcccgcgtag
      601 gacacggaga agcccttgcg cacctcgtcc accttgggga tggtcaccgt ctccccggcc
      661 ttaagctcca ggggcgccgg caggcccttt acgcggacct ccgggccctt ggtgtccacc
      721 aggatgccta tcgtatcgga caccttcctg atattggcca ccaccttggc ggaggcttcc
      781 ggcgtctggt gggcggtatt gagcctgacc acgttcatgc cggcctcgta gagcgagcgg
      841 aggaactccg gctcgcactg ggaatccgat atggtcgcga cgatcttggt gagtttctgc
      901 gtgttcatgc tacaagtatc cgcaaaacgg cgacgaaatg gaagactggt tcctcgaagc
      961 ttgtggagcg tcgcgggacg ccgcgatagg cgggccggcg tcttcgccgg gctaccagat
     1021 cctgtagcga cggcttcttc gccacaagct tcgaggaact aggtatcgag cacggccgac
     1081 aggccgtcgg ataacggccc gccaagcgat acgagcggcg ctatcgctcc aggaagccgg
     1141 ctacgcgctc cacgacgtcg aggcgatcgg ggccgaggag caggttatgc tcgccgccgt
     1201 cgtaacgctg gacccgctcg atcgaccgga tcgacgttgc ggcctcgtct atccgcgcgg
     1261 gg
//
LOCUS       DAOOLN010000762         1260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_145319_length_1260_cov_3.785892, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000762 DAOOLN010000000
VERSION     DAOOLN010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145319_length_1260_cov_3.785892"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PLW80_09420"
     CDS             complement(<1..649)
                     /locus_tag="PLW80_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPB66772.1"
                     /translation="MSGLAPWAVAALVPYAALQAAFLRRLATVPEALGGTMEANGRAR
                     WRRYVARRAVGAAFGAVFWVLCALAASGAVRVPRYATQTVTGAELAFVLDASNSMLSA
                     DEGVSRLERASDFVSRLAAAADGAGLSVIAFRGRPVTLCPSTRDRKAFADALLWAGPA
                     VTSAAGSDVGAAIDEALRPAFPPGAARLVIVLSDGNDTGGRAKAAAAEAAASGAAL"
     gene            complement(646..>1260)
                     /locus_tag="PLW80_09425"
     CDS             complement(646..>1260)
                     /locus_tag="PLW80_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPB66773.1"
                     /translation="NRMGFNNDGAEVVAERLALIRPGILGDGTAIGQGLASAYRQVKA
                     SGAARALVVLLSDGEDNVGLVHPADAARAVAASGAALLVVGLGSRGDVPIDYVDPDSG
                     QRMSGAYRSGFDDAALAAVAAAGGGEYRSAGDAKSLEALAATLGDSVSGLAPSERSED
                     GGRVSAPGPADSSRRPVGRALVLAAMALAAVGWALRALALGGLA"
BASE COUNT          172 a          482 c          466 g          140 t
ORIGIN      
        1 ggagggccgc cccggaagcc gcggcttcgg cggccgcggc cttggcgcgt ccgccggtat
       61 cgttcccgtc gctgaggacg atcacgagcc tggcagcgcc gggagggaag gcgggcctga
      121 gcgcctcgtc tatcgcggcg ccgacgtccg aaccggccgc gctcgtcacc gcggggccgg
      181 cccaaaggag ggcgtcggcg aaggccttgc gatcgcgggt ggacgggcag agggtgactg
      241 gacggcccct gaacgctatc accgacaggc cggcgccgtc ggcggcggcc gccagtctcg
      301 agacgaaatc gctggcccgc tcaagccggc tcactccctc gtcggcgctc agcatcgagt
      361 tggacgcgtc gaggacgaag gccagctcgg ccccggtcac cgtctgggtg gcgtagcgcg
      421 gcacgcgtac cgccccggag gcggccaagg cgcatagcac ccagaagacc gccccgaacg
      481 ccgcgccgac ggcccgtcgc gccacatagc gacgccagcg ggcgcgaccg ttcgcctcca
      541 tcgtaccgcc tagggcctcc gggaccgtgg cgagccgcct caggaacgcg gcctgcaggg
      601 ccgcgtacgg aaccagcgcg gcgactgccc agggcgcgag gccgctcacg cgagcccccc
      661 gagcgccagg gcgcgtagcg cccagccgac ggcggccaag gccatcgcgg ccagtaccag
      721 ggcgcggccc accggccggc gcgacgagtc ggccggtccc ggcgcggaga cccggccgcc
      781 gtcctcggag cgctcgctcg gcgcgagccc ggacacggaa tcgccgagcg tcgccgccaa
      841 ggcctcgagg cttttcgcgt ctccagccga gcggtactcg ccgccgccgg cggcggcgac
      901 ggccgccagc gccgcgtcgt cgaagccgga gcgataggcg ccgctcatgc gttggccgct
      961 gtccggatcg acgtagtcga tcggcacgtc gcctcgggag cctaggccga ccacgagcag
     1021 cgcggccccg gaggcggcga cggctcgcgc cgcgtcggcc ggatgcacga ggccgacgtt
     1081 gtcctccccg tcgctcagga gcaccacgag ggcgcgcgcg gcacccgagg cttttacctg
     1141 gcggtacgcg ctcgccagcc cctggccgat ggcggtgccg tcgccgagga tgcctggccg
     1201 tatcagggcg agccgctcgg ccacgacctc cgccccatcg ttgttgaatc ccatgcggtt
//
LOCUS       DAOOLN010000763         1260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_145341_length_1260_cov_3.146058, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000763 DAOOLN010000000
VERSION     DAOOLN010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145341_length_1260_cov_3.146058"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PLW80_09430"
     CDS             complement(<1..480)
                     /locus_tag="PLW80_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HPB66774.1"
                     /translation="MGPGQAYAIGKGWEGMGFEGAGSVGLTMAALGYLWACVVGVALV
                     NLGVRCGYLPKNAQTAMADRAMLTGIMPREREKPIGSRNTTDLEAIDPMSLHAAAVAC
                     TYILSFLLLKALTWALGFAGNAGRELAVNLWGMNFIFSALTAMLVRAIVDKLRIGYVL
                     "
     gene            complement(501..905)
                     /locus_tag="PLW80_09435"
     CDS             complement(501..905)
                     /locus_tag="PLW80_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66775.1"
                     /translation="MDFSWKIIIDAGIISAALIVATFLRTKLRFLQRFMVPNSLTAGV
                     LLLPVYNYLMPALGYTQNRLGDLVYHLLNISFIAMTLRSKPKGPAKDSSHSVWAMSTA
                     IISQYSLQALFGLLLTWVLFSTVLPGLNPAFG"
     gene            1026..>1260
                     /locus_tag="PLW80_09440"
     CDS             1026..>1260
                     /locus_tag="PLW80_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66776.1"
                     /translation="MDFNRFSEGVRELVEKTQLFGSEPLEAVLLANPRAGGFSRPARA
                     AALTRDLAQAVSDASALPARSASLSWRVAQTSGP"
BASE COUNT          243 a          388 c          420 g          209 t
ORIGIN      
        1 gaggacgtag ccaatccgta gcttatcgac gatggctcgc acgagcatgg cggtcagggc
       61 ggagaagatg aagttcatgc cccagaggtt cacggccagt tcgcgaccgg cgttcccggc
      121 gaagccgagc gcccaggtca gggccttgag cagcaggaac gacaggatat aggtacaggc
      181 taccgcggcg gcgtggaggc tcatcgggtc gatcgcctcg aggtcggtcg tgttcctgga
      241 gccgatcggc ttctcccgct cccttggcat gatgccggtt agcatggcgc ggtcggccat
      301 ggccgtctgg gcgttcttag gcaggtagcc gcacctgacg ccgaggttga cgagggccac
      361 gccgacgacg caggcccaga ggtaaccgag ggcggccatc gtcaggccca ccgaaccggc
      421 gccctcgaag cccatgcctt cccagccctt gccgatcgcg taggcttggc cgggtcccat
      481 cgcgaagccg agcggtagcg tcagccaaag gccgggttca ggcccggcaa cacggtcgag
      541 aacaggaccc aggtcagcag gagcccgaac agggcctgca aggagtactg cgagatgatg
      601 gccgtcgaca tggcccagac cgagtgggac gagtccttgg ccggcccctt cggcttcgag
      661 cgtagcgtca tggcgatgaa cgatatattg agtaggtggt agaccaggtc gccgagccgg
      721 ttctgggtat agccgagggc cggcatcagg taattgtaga cgggcagcag gagaacgccc
      781 gccgtcagcg aattcggaac catgaagcgc tgcaagaagc ggagtttcgt ccgcaagaag
      841 gtggcgacga taagcgccgc ggagatgatg ccggcgtcga tgatgatctt ccatgagaag
      901 tccatagtag tcctttcccg cctaacggcc agagccggcc ctcggcgcct cgacgacgag
      961 tctaccatac gtatggcgat agagaacgct tttcaaccga aggcttccgt agtacactcc
     1021 ctgccatgga ttttaaccgc ttctcggaag gcgttcgtga actagtcgaa aagacgcaac
     1081 tcttcggatc ggagccgctc gaggcggtac tgctcgctaa tcccagggcc ggcggtttct
     1141 cgaggcccgc cagggccgcc gcgctgacgc gcgacctcgc gcaagcggtc tctgacgctt
     1201 cagccctgcc ggctcggtcc gccagcctgt cgtggcgggt agcccagacc tcgggcccca
//
LOCUS       DAOOLN010000764         1258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_145787_length_1258_cov_1.923525, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000764 DAOOLN010000000
VERSION     DAOOLN010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145787_length_1258_cov_1.923525"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PLW80_09445"
     CDS             <1..538
                     /locus_tag="PLW80_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB66777.1"
                     /translation="MSVAVLGISVPAFVVGPILMLIFALKLKWLPTSGWLSSRAGALT
                     LIMPIITLMMPYFAYIARMSRGSLIEVMRSDYVRTARAKGLKESVVIRKHVLKGAMLP
                     IVTYLGPAFSGIITGSVVVEQIFVIPGIGRIFVQSALNRDYTVIMGDVIVYSIILIAA
                     NFVVDILYGLLDPRISYK"
     gene            552..1028
                     /locus_tag="PLW80_09450"
     CDS             552..1028
                     /locus_tag="PLW80_09450"
                     /inference="COORDINATES: protein motif:HMM:NF024313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66778.1"
                     /translation="MPSIDKNNDRATNEFNQYVKPVSLWADAWKRLRRNKMAVLGMWI
                     VAIYMVVTFGAALLPFKSYTEQEMLHINLPPSFKPAGVVALGRLEERKVELEAAITRS
                     GRADLKQDLTRIENEIIQLKGEVDTDPKHKRVYILGTDYLGRDMLARTIYGVRYRS"
     gene            1025..>1258
                     /locus_tag="PLW80_09455"
     CDS             1025..>1258
                     /locus_tag="PLW80_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB66779.1"
                     /translation="MIGLIGTLTAAMIGIVLGSLGGYLGGWVDNLIGRFVDLMYSLPY
                     MLVVIIIMSMLPTGSNSIFVLYIAIAMISWLTIS"
BASE COUNT          242 a          403 c          364 g          249 t
ORIGIN      
        1 gatgtcggtg gccgtgctcg gtatctcggt gccggccttc gtcgtcggac cgatactgat
       61 gctcatcttc gcgctcaagc tcaaatggct accgacgtcc ggctggctat cgagtcgcgc
      121 cggcgccctg accctgatca tgcccatcat caccctgatg atgccctatt tcgcctatat
      181 cgcgaggatg tctcgcggct cgctgatcga ggtgatgcgc tccgactacg tgcggaccgc
      241 ccgggccaag ggcctcaagg agagcgtggt catccgcaaa cacgtgctca agggcgcgat
      301 gctgccgatc gtcacctacc tcgggccggc cttctccggt atcatcaccg gatcggtcgt
      361 cgtggagcag atcttcgtga taccgggcat cggccgcatc ttcgtgcagt cggcgctgaa
      421 ccgcgactac acggtgatca tgggcgacgt catcgtctac tcgatcatcc tgatcgcggc
      481 caacttcgtc gtggatatcc tgtacggcct cctcgacccg cgcatctcgt acaaataagg
      541 agccatcgga tatgccgtca atcgacaaga acaacgatag ggctaccaac gaattcaacc
      601 agtacgtgaa gccggtctcg ctgtgggcgg acgcgtggaa acgcctgcgc cgcaacaaga
      661 tggccgtact cggtatgtgg atcgtcgcca tctatatggt cgtcaccttc ggggcggccc
      721 tgctaccgtt caagagctac accgagcagg agatgctcca catcaacctg ccgccgtcct
      781 tcaagccggc cggcgtcgtg gcgctcggtc ggctcgaaga gcgcaaggta gagctcgagg
      841 ccgccatcac gcggagcgga cgggccgacc tcaagcagga cctgacgcgc atcgagaacg
      901 agatcatcca gctcaagggc gaggtcgaca ccgatcccaa gcataagcgc gtctacatac
      961 tcggtaccga ctacctgggc cgcgatatgc tcgccagaac catctacggg gtcagatatc
     1021 gatcatgatc ggcctcatcg gcacgctgac cgcggcgatg ataggcatcg tgctcggctc
     1081 gctcggcggc tacctgggcg gctgggtcga caacctgata ggccgcttcg tcgacctgat
     1141 gtacagcctg ccgtacatgc tcgtcgtcat catcatcatg tcgatgttgc cgaccgggtc
     1201 gaacagcatc ttcgtgctgt acatcgccat agcgatgata tcgtggctga ccatctcc
//
LOCUS       DAOOLN010000765         1257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_145914_length_1257_cov_2.636439, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000765 DAOOLN010000000
VERSION     DAOOLN010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145914_length_1257_cov_2.636439"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="PLW80_09460"
     CDS             complement(<1..1090)
                     /locus_tag="PLW80_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPB66780.1"
                     /translation="MSGRKPVFYASALIGLAAGAAALIMKALVHAVSAAGSGLVEAVG
                     WPLLRYVLPLVGIALTTVFTRYVIRDDIGHGVAKVLKARARDKGDIAPHNMYSSIIGC
                     SLTVGFGGSVGLEAPVMYTGSAIGSNVAKLLRLDAKTKTILVGCGSAAAVAAIYKAPI
                     AGIVFALEVLMIDSTPGNKLPIIVSSMIGALVSMLATGTGVSYSFAVHDSLNLAGLPY
                     YVGLGAFCGLLALHLHKTSKAMETLLKRAASPWLKAAIGGLGVGLLAALLPPLFGEGS
                     WALEAVLSGRPFDALAGDPFGAGLPSDRRLLLFFAAVLVVKPVAAGLTTGAGGVGGVF
                     APSLFMGGIAGSLFARAARLLGAASVSEA"
BASE COUNT          220 a          408 c          446 g          183 t
ORIGIN      
        1 tggcctcgga tacggaggcg gccccgagga gcctggccgc gcgtgcgaac agtgagccgg
       61 cgatgccgcc catgaacaga ctcggcgcga acacgccccc gacgccgccg gccccggtcg
      121 tcagcccggc cgcgaccggt ttgacgacga gtacggccgc gaagaagagg agcagccgtc
      181 ggtccgacgg gagtcccgcc ccgaacgggt cgccggccag cgcgtcgaag ggccgcccgg
      241 acagcaccgc ctcgagcgcc cacgaacctt ctccgaacag cggcggcaga agggctgcga
      301 ggaggcctac tccgaggccg ccgatggctg ccttgagcca gggcgaggcc gcccgcttga
      361 ggagcgtttc catggccttg ctcgtcttgt gcaggtgcag ggccaggagc ccgcagaagg
      421 ccccgagccc gacgtagtag ggtagcccgg cgaggttcag gctatcgtgg acggcgaagg
      481 agtacgatac gccggtgccc gtcgccagca tgctgacgag ggcgcctatc atcgacgaga
      541 cgatgatcgg tagtttgttg ccgggcgtcg agtctatcat gagcacctcg agcgcgaaga
      601 cgatgccggc tatcggagcc ttatagatag cggcgacggc cgcggccgag ccgcagccga
      661 cgagtatggt cttggtcttc gcgtcgaggc ggagtagctt ggctacgttc gagcctatcg
      721 ccgagcccgt gtacattacc ggcgcctcga ggccgaccga gccgccgaaa ccgacggtca
      781 gggaacagcc gatgatggac gagtacatat tatggggcgc gatgtcgcct ttgtcgcggg
      841 cccgcgcctt gagcaccttg gcgacgccgt ggcctatgtc gtcccggatg acgtagcggg
      901 tgaagaccgt cgtcagcgct atcccgacga ggggcaggac gtaacgtagg agcggccagc
      961 cgaccgcctc gacgagcccc gagccggcgg ccgagacggc gtgtacgagc gccttcatga
     1021 tcagggccgc ggcgccggcg gccaggccta tgagcgcgct cgcgtagaag accggcttac
     1081 ggcccgacat gagcgccgcc gtcgccttcc tcgcggtatc gatcatcctg gatgagtcca
     1141 tatcgatcag gatagcctcg atggacggcg gggtaaaggc ctcgcgttct cacgaggcgg
     1201 ccgcccgtgc tatactcggc ttggcgcccc tccacggggc ggcgaaaaag gagattt
//
LOCUS       DAOOLN010000766         1257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_145920_length_1257_cov_2.562396, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000766 DAOOLN010000000
VERSION     DAOOLN010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145920_length_1257_cov_2.562396"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..>1257
                     /locus_tag="PLW80_09465"
     CDS             216..>1257
                     /locus_tag="PLW80_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPB66781.1"
                     /translation="MRAIAANRLTGSRLALLAIAFYLCALPCAFAEDTAAPDGRSQPR
                     RVVEAGGDWAYAPFEYIDEAGYPAGFNVDIMRAIGDAVGIGFDIRLSSWAEARERIER
                     GDIDLLMGMYKSPERAEAVDFSSPHFYNVYGLFARKGSSVESLDDLIGRRVAVQDGDR
                     GHDYAVESGLGSNLVALRDWRDVFGAILRGEADYAIASALQGSIAVKQPEFRSIEMVG
                     PPLFSAEYCVAVRKGDEELLALIDEGLARIRSSGEYDTIYMRWFGHDLGPGGNPRLGI
                     VAAVATAALTVIAAALIWALSLRRLLTERTAELSAEQRRSREAGSKLQAALAEADEAA
                     RKVLGSVSPISDV"
BASE COUNT          218 a          397 c          430 g          212 t
ORIGIN      
        1 ggccgtacgg accgtagtca gcaccgagac tatccccgcc acgctgaaca gcagggcgga
       61 tacgccgatc acaggtagca gtatcttcgt acgtatggtc aaggctgcct cctacctctc
      121 agtatacccg tttttgccca atagtaggaa tagtttgcgc ggcgagttgg aatgacgtat
      181 cattcatggc gatgacatcg tacgatagaa aagccatgcg cgcgatcgcc gcaaaccggc
      241 taacgggaag ccgcctcgcg ctcctggcca tagcgttcta tctgtgcgcc ttgccatgcg
      301 ccttcgccga ggatacggca gccccggacg gccggagcca gccgcgccgc gtcgtggagg
      361 ccggcgggga ctgggcctat gcgcccttcg agtacatcga cgaggcgggc tatccggcgg
      421 gcttcaatgt cgacatcatg agggcgatag gcgacgcggt gggtatcggc ttcgatatac
      481 ggctatcgtc ctgggccgag gcccgggagc ggatcgagcg aggcgacatc gacctgctca
      541 tgggcatgta caagagcccc gagcgcgccg aggcggtcga tttctcgtcg ccgcatttct
      601 ataacgtcta cggactattc gcgcgcaagg gctcgagcgt cgagtcgctc gacgatctga
      661 tcggccgtcg ggtcgccgtc caagacggcg accgtggcca tgactacgcg gtcgagagcg
      721 ggctcggctc gaacctcgtc gcgctacgcg actggcgcga cgtgttcggc gccatcctgc
      781 ggggagaggc ggactatgcc attgcgtcgg ccctgcaggg ctccatcgcg gtcaagcagc
      841 cggaattcag gtcgatcgag atggtcggcc ccccgctatt cagcgccgag tactgcgtgg
      901 cggtcaggaa gggcgacgaa gagctgctgg cgctgatcga cgagggtctg gcgcggatac
      961 gctcgagcgg cgagtacgac acgatctaca tgcgatggtt cggccacgac ctgggccccg
     1021 gaggaaaccc gcggctcggg atcgttgccg ccgtggcgac cgcggccctg accgtaatcg
     1081 cggccgcgtt gatctgggcc ctgtcgctgc gccggctcct aaccgaacgg acggccgagc
     1141 tatccgccga gcagaggcgc tcgcgcgagg cgggctcgaa gctgcaggcg gcgctcgccg
     1201 aggccgacga agcggcgcgc aaggtgctcg gctcggtctc tcccatcagc gacgtgc
//
LOCUS       DAOOLN010000767         1256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_146096_length_1256_cov_2.938385, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000767 DAOOLN010000000
VERSION     DAOOLN010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146096_length_1256_cov_2.938385"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PLW80_09470"
     CDS             <1..573
                     /locus_tag="PLW80_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66782.1"
                     /translation="RLPGSADGKEAFGAPAAAPSDRSGVSVRRVDPLDASAAGRIRAA
                     LTAGPWAAGFRLESWKAYPLEASPAIREDDLILEAVGSDGSVLGRLIGSLDPLKAQAN
                     VVAFGLEPSSPSDASSASSDGLAAAALFAAFERIASDAGYAYFEAIAPGLAGQREALD
                     ACGYESWESEDDFLLYEWPAERLGELARPR"
     gene            complement(570..>1256)
                     /locus_tag="PLW80_09475"
     CDS             complement(570..>1256)
                     /locus_tag="PLW80_09475"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HPB66783.1"
                     /translation="PAFASLSDDEGSALRRMASWFVDVKDFVTLGGARIDEADEATIA
                     LFACLPVLRLDPRWYDDWSTILAAPDGFVHRMTSVDGAGVVTEYDDLLSGRVTELGPV
                     LLSLPDVRESGFGDGYNVVVHEMAHKLDERDGGLDGCPPLPRSMSRRAWRAAFGDAYA
                     DFQVRVARSSSGRRVGRASRLPLDEYAAESPDEFFAVACEAFFDAPQRLEAAYPEVYE
                     LLGRFFSGGA"
BASE COUNT          180 a          464 c          440 g          172 t
ORIGIN      
        1 cgactgcctg gctcggcgga cggcaaggag gccttcggcg ccccggccgc cgcgccgtca
       61 gaccgctccg gcgtatccgt ccgccgcgtc gacccgctcg acgcctcggc cgccgggcgg
      121 attcgagccg ccctgaccgc cggaccctgg gccgccggct tcaggctgga gtcgtggaag
      181 gcctacccgc tcgaggcgag cccggcgatc agggaagacg acctgatcct cgaggcggtc
      241 ggtagcgatg ggtccgtcct cggacggctc atcggctcgc tcgatccgct caaggcccag
      301 gccaatgtcg tcgccttcgg cttggagcct agctctccat cggacgcgtc ctcggcctcg
      361 agcgacggcc tggccgccgc cgcgctgttc gcggccttcg agcggatcgc ctccgacgcc
      421 ggctacgcgt acttcgaggc catcgcgccg ggcctcgcgg gccagcgcga ggccctcgac
      481 gcctgcggct acgaaagctg ggagagcgag gacgacttcc tcctctacga gtggccggcc
      541 gagcggctgg gcgagctggc gaggccgcgc taggccccgc ccgagaagaa gcgcccgagg
      601 agctcgtaga cctcggggta ggcggcctcg aggcgctgcg gtgcatcgaa gaaggcctcg
      661 caggcgaccg cgaagaactc gtcggggctc tcggccgcgt actcgtcgag cggcagtctc
      721 gaggctcggc ccacacgcct cccgctcgac gaacgggcca cccgcacctg gaaatcggcg
      781 taggcgtcgc cgaaggcggc gcgccaggcg cgccggctca tcgacctggg caggggcggg
      841 cagccgtcta ggcctccgtc ccgctcgtcg agcttgtggg ccatctcgtg caccacgacg
      901 ttgtagccgt cgccgaagcc cgactcgcgc acgtccggca acgagagcag cacggggccg
      961 agctcggtga ccctgccgga cagcaggtcg tcgtactcgg tcaccacgcc tgcgccatcg
     1021 acgctcgtca tcctatggac gaagccgtcc ggggcagcga gtatcgtcga ccagtcgtcg
     1081 taccagcgcg ggtcgagcct gagcaccggc aggcaggcga atagcgcgat ggtcgcctcg
     1141 tccgcctcgt cgatccgggc tccgccgagc gtcacgaagt ccttgacgtc gacgaaccac
     1201 gaggccatgc gcctgagcgc cgatccctcg tcgtcgctca gcgacgcgaa ggccgg
//
LOCUS       DAOOLN010000768         1256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_146170_length_1256_cov_2.140716, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000768 DAOOLN010000000
VERSION     DAOOLN010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146170_length_1256_cov_2.140716"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PLW80_09480"
     CDS             complement(<1..268)
                     /locus_tag="PLW80_09480"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HPB66784.1"
                     /translation="MHIALPYDKGRIELEVADDRVAGVLTVPRHAAGGRSEAEIVRAA
                     HRAPLGSKPLSELAVGKKRVVVITSDHTRAVPSALTLPILLEEIR"
     gene            complement(488..>1256)
                     /locus_tag="PLW80_09485"
     CDS             complement(488..>1256)
                     /locus_tag="PLW80_09485"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPB66785.1"
                     /translation="QGHALPPAALSSNRVTKPFIAKYTIFLYSSGMALHGSGRIISTI
                     KDQVYSVIKENILNENLKPGQRIQELQIAKELNVSRSPVRSAINELIGEGLLESIPNK
                     SVTVRRMSEKDILDAYEFRLVVEGFAVQRVIELMDDDIAATLARFREQFTATVDYGQI
                     QEYVRIDTAFHEYLVNTAGNTIFKEALGRVAMMITPFRIFSLSSQQRFLDSIREHTGL
                     IDAILGRDADRASDICNAHLRLAKEEIIKHLRSSIIA"
BASE COUNT          241 a          359 c          386 g          270 t
ORIGIN      
        1 tgcgtatctc ctcgagcagg atgggcaggg tcagggcgct cggcacagcg cgcgtatggt
       61 cgctcgtgat gacgacgacg cgctttttcc cgacggccag ctcggacagc ggcttcgagc
      121 ccaggggagc gcgatgcgcg gccctaacga tctccgcctc gctccgcccg ccggccgcat
      181 gccgcggcac ggtcagcacg ccggcgaccc tatcgtcggc gacctccagt tcgatacggc
      241 ccttgtcata gggcaaggct atatgcatag cgactccttc gcgacagttc gttcgtatgg
      301 gataactgca tgaagtgtat tatgtataca taatacactg tctttatatc ttgtcaaccg
      361 ttaaccgagt gaggggtagc cgttgggatg gattggcatg aggtgggcgc gcctcgccca
      421 gggccgtcgc cctggcgcgg cgacgctacg ggaggatgac gagcgtctcg aactatacgg
      481 aggctggcta cgcgatgatc gatgaacgca ggtgcttgat gatctcttcc ttagccaggc
      541 gcaggtgggc gttgcagata tccgaagccc tatcggcgtc gcgtccgagt atggcgtcga
      601 tgaggccggt gtgctcccgt atcgagtcga ggaagcgctg ttggctcgac aacgagaata
      661 tcctgaaagg agtgatcatc atcgcgacgc gaccgagggc ttccttgaag atcgtgttac
      721 ccgccgtatt gacgaggtac tcgtggaagg ccgtatcgat ccggacgtat tcctggatct
      781 ggccatagtc gacggtcgcc gtaaactgct ccctgaaacg ggccagagtg gccgctatat
      841 cgtcgtccat gagctcgatc acccgctgta cggcgaagcc ctcgacgacg agcctgaact
      901 cgtacgcgtc gaggatatcc ttctccgaca tcctgcgcac cgttacgctc ttattgggaa
      961 tggactcgag caggccttcg ccgatgagct cgttgatggc gctgcgcacg ggagagcggc
     1021 tgacgttcag ttccttggct atctggagtt cctggatgcg ctgcccgggc ttcaggttct
     1081 cgttgaggat attctccttg atgacggagt acacctgatc cttgatcgtc gaaatgatcc
     1141 gcccgctacc atgcaatgcc atgccgctag aatacagaaa aatggtatac ttggcaatga
     1201 acggcttcgt aacccgattc gacgacagcg ccgctggagg tagagcatgg ccctgc
//
LOCUS       DAOOLN010000769         1255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_146366_length_1255_cov_1.939167, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000769 DAOOLN010000000
VERSION     DAOOLN010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146366_length_1255_cov_1.939167"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLW80_09490"
     CDS             complement(<1..293)
                     /locus_tag="PLW80_09490"
                     /inference="COORDINATES: protein motif:HMM:NF025044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HPB66786.1"
                     /translation="MDSAEAIYKHTLSALGVDIDGGFRPTYVMVPEKAPVIAKIAEAA
                     RAAQQIYLATDPDREGEAIAWHLQEAAGLDPERTQRISFNQVTPEAVRQAMQAP"
     gene            425..1144
                     /locus_tag="PLW80_09495"
     CDS             425..1144
                     /locus_tag="PLW80_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB66787.1"
                     /translation="MSKLSTTGLTKAFSGLVAVNDVSFAMEEGEILGIIGPNGAGKTT
                     FIHLVSGILMPSSGRIEFKGRDVTYLPAHERSRLGIARTYQLIHPLENLKVLENVMVG
                     AVFSRGKSLREARLSAEGICDELGLGGLDRDTAQLSILEIKKMEIARALANEPEVLFL
                     DEVMAGLNSDETKQMIETVRRIAGERRMAVGVVEHVMGVIRELTQRVIVLEAGELIAE
                     GPYEKVSKDPRVIEAYLGGAA"
BASE COUNT          221 a          399 c          445 g          190 t
ORIGIN      
        1 ggcgcctgca tcgcctggcg caccgcctcg ggcgtcacct ggttgaagct gatgcgctgc
       61 gtgcgctctg gatcgagccc ggcggcctct tgcaggtgcc aggcaatggc ctcgccctcg
      121 cgatccgggt cggtggcaag atagatctgc tgagcggcgc gggccgcctc ggcgatcttg
      181 gcgatcaccg gcgctttctc aggcaccatc acataggtgg ggcgaaatcc cccgtcgata
      241 tcgacgccca gcgcggagag cgtgtgcttg tagatcgcct cggcggaatc catcgcgacc
      301 gccagatcgc cgatataggg ccgaacggcc ttttcggcct ggcccggaag gcgatacgca
      361 aggccggcgg ggcgggcagc ccgggctcgg cctcgaacgg gtcggacgcc gaggagggcg
      421 cgcgatgagc aagctgagta cgaccggcct gaccaaggcc ttctccggcc tcgtggccgt
      481 caacgacgtg agcttcgcga tggaggaggg cgagatcctc ggcatcatcg gccccaacgg
      541 cgccggcaag acgaccttca ttcacctggt gtccggcatc ctgatgccga gctccgggcg
      601 gatcgagttc aagggccgcg acgtcaccta cctgccggcc cacgagcgta gccgcttggg
      661 catagccagg acctatcagc tgatccaccc gctcgagaac ctcaaggtgc tcgagaacgt
      721 gatggtcggc gccgtcttca gtcgaggcaa gagcctcagg gaggccaggc tctcggccga
      781 gggcatctgc gacgagctag gcctgggcgg gctggaccgc gataccgccc agctgtccat
      841 cctcgagatc aagaagatgg agatagcccg cgccctggcg aacgagcccg aggtgctgtt
      901 cctcgacgag gtgatggccg ggctcaacag cgacgagacc aagcagatga tagagaccgt
      961 caggcgcatc gccggcgagc gcagaatggc ggtcggggtc gtggagcacg tcatgggcgt
     1021 catccgcgag ctgacacagc gcgtgatcgt gctcgaggcc ggcgagctga tagccgaggg
     1081 accgtacgag aaggtctcca aggacccccg cgtgatcgag gcctacctcg gaggagccgc
     1141 atgatactgg acatcaagaa gctcgagact ggctacggca agctgcgggt gctccgcggc
     1201 gtggacctgt ccgtgggccg cgagtcggtg ggcctgttcg gcccgaacgg ggcgg
//
LOCUS       DAOOLN010000770         1254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_146526_length_1254_cov_2.300250, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000770 DAOOLN010000000
VERSION     DAOOLN010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146526_length_1254_cov_2.300250"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PLW80_09500"
     CDS             <1..327
                     /locus_tag="PLW80_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66788.1"
                     /translation="AWDEDEHRALTGASNRAVITNLRFLAAAGKLYEVRTVVVPGSFD
                     AERTVREVSRALVDSGSAARYKLIRFRPQGVRARWRGLPVPDDDTMAALAALARAEGV
                     REVVVV"
     gene            391..1179
                     /locus_tag="PLW80_09505"
     CDS             391..1179
                     /locus_tag="PLW80_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPB66789.1"
                     /translation="MKNTQRQELAVCGLESVRALAAVRPDAVKRFFYTEERAREFGGL
                     CKRLASLRRPYRLVGPDELAKLSGTAHHQGVVAMVEDSPPRPVDDALVASWAASGARI
                     VALDRVGDDHNLGSIARSAAFFGWTAIVLGRDDGAARLSTSAYRVARGALERIQVYAD
                     ESMARFVSRCGGSLAVVGADHRGTMGLREARAALVGGGSPAGSGGSARGLAVVLGNEE
                     TGLSAPVRAACSSLVRIDGSGAVESLNVSQAAAVLLYELGPGGA"
BASE COUNT          158 a          452 c          456 g          188 t
ORIGIN      
        1 gcctgggacg aggacgagca ccgcgcgctg accggcgcgt cgaaccgggc ggtgataacc
       61 aacctgcgct tcctggccgc cgccggcaag ctgtacgagg tccgcacagt cgtcgtcccg
      121 gggagcttcg atgccgaacg gaccgtgcgc gaggtatccc gggcgctggt cgatagcggc
      181 tcggcggcgc gctacaagct gatacgcttc aggccgcagg gcgtgcgcgc gcgctggcgc
      241 ggcctgccag ttcccgatga cgatacgatg gcggcccttg ccgccctggc cagggccgag
      301 ggcgtccgcg aggtcgtcgt cgtttgaccc gtcgtctccc gcccaggggc ggcgcttgct
      361 aagcgagcgc ccggcggcta tcctgtcgcc gtgaagaata cccaacgcca ggagctggca
      421 gtctgcggcc tcgagtcggt ccgcgcccta gccgccgtca ggccggacgc ggtcaagcgc
      481 ttcttctata ccgaggagcg ggccagggag ttcggcgggc tatgtaagcg gctcgcctcg
      541 ctgcgccgac cgtaccgcct cgtcggcccg gacgaactgg ccaagctgtc gggcaccgcc
      601 catcaccagg gcgtcgtcgc catggtcgag gactcgcccc cgaggcccgt cgacgacgcg
      661 ctcgtcgcct cgtgggcggc ctccggcgcc aggatagtcg cgctcgaccg ggtcggggac
      721 gaccataacc tcggctcgat agcccgctcc gccgccttct tcggctggac ggccatcgtg
      781 ctcggccgcg acgacggggc ggcccgcctg tcgacgagcg cgtaccgcgt ggcccgcggc
      841 gccctcgagc gcattcaggt ctacgccgac gagtcgatgg cccgcttcgt gtcgcgatgc
      901 ggcggctcgc tcgccgtggt cggcgcggac catcgcggga ctatgggcct ccgcgaggcc
      961 cgagcggcct tggtcggcgg cggaagtccc gcgggctcgg gcggctccgc gcgaggccta
     1021 gccgtggtgc tcggcaacga ggagaccggc ctgtccgcac cggtacgcgc tgcctgctcg
     1081 agcctcgtcc ggatcgacgg ctcgggcgcc gtcgagagcc tcaacgtttc ccaggccgcg
     1141 gccgtgctcc tgtacgagct cgggcccggc ggcgcctagg ctcggggagg atcgcaccgt
     1201 gggccgtcgg cgatcgatgc gggtcatgct cgatacaaac gtcttcgatc ggct
//
LOCUS       DAOOLN010000771         1254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_146554_length_1254_cov_2.001668, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000771 DAOOLN010000000
VERSION     DAOOLN010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146554_length_1254_cov_2.001668"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="PLW80_09510"
     CDS             complement(<1..873)
                     /locus_tag="PLW80_09510"
                     /inference="COORDINATES: protein motif:HMM:NF019353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPB66790.1"
                     /translation="MVRDIVRRASRSCGRGSAAASVVSYALGITDVDPIRHGLYFERF
                     LNPGRADPPDIDVDFAWDERDGLLESVLAEYGEARAARVSNHVRFQARSALREAARAY
                     GMPDGEISARERSAASADPGGAGRDAPRDGEWAEIARLAARLPGLPRHLGVHSGGVIL
                     VPDELASRVPVERSGSGIRVTAWDKDGVEAAGLVKIDLLGNRSLAVVRDALVNLEENG
                     EPIDERAWRPIDDPGTIAMLARGDAMGVFYVESPAMRLLQKKTGRGDFEHLVIHSSII
                     RPAANRYISEYIDRL"
     gene            957..>1254
                     /locus_tag="PLW80_09515"
     CDS             957..>1254
                     /locus_tag="PLW80_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66791.1"
                     /translation="MGADAIASDGALERQAAQRRYRIVALLSARPGVAREDEAVERRA
                     ERDGADERLVGGKRFGCSGVRGAEPGRIDEGSALGRQLVVADLVDGSPGAYGDEQ"
BASE COUNT          187 a          425 c          464 g          178 t
ORIGIN      
        1 cagccggtcg atgtactccg agatgtagcg gttggccgcc gggcgtatga tcgacgaatg
       61 gatgaccagg tgttcgaagt cgccgcggcc ggtcttcttc tggaggaggc gcatcgccgg
      121 cgactcgaca tagaagacgc ccatcgcgtc gccgcgcgcg agcatggcta tcgttcctgg
      181 gtcgtcgatg ggccgccacg cgcgctcgtc gataggctcg ccgttctcct ccaggttgac
      241 gagggcgtcc cgcacgacgg cgagcgagcg gttcccgagc aggtctatct tgacgaggcc
      301 cgccgcctcg acgccgtcct tgtcccaggc ggtcacgcgt atgcccgaac cggagcgctc
      361 gaccggcacg cgcgaggcga gctcgtcggg gaccaggatg acgccgccgg aatggacgcc
      421 gaggtggcgg ggcagacccg gcagcctggc ggcgagacgg gcgatctcgg cccattcgcc
      481 gtcgcgtgga gcgtccctcc cagccccgcc agggtcagcg ctggcggcgc tccgctcccg
      541 ggcggagatc tcgccgtcgg gcataccgta ggctcgggcg gcctcgcgca gggcggagcg
      601 ggcctggaag cggacgtggt tcgagaccct ggcggccctg gcctcgccat actcggcgag
      661 caccgactcg agtagcccgt cgcgctcgtc ccaggcgaag tcgacgtcga tatccggagg
      721 gtcggcccgg ccggggttga ggaagcgctc gaagtacagg ccatgccgta taggatcgac
      781 gtcggtgatg ccgagagcgt acgagacaac gctggctgcg gcgctgccgc ggccgcagct
      841 gcgcgaggcg cgtcgcacga tgtcgcggac cacgaggaaa tagtccgcga agcccttctg
      901 gcgatgatct ccagctcgta atctatccgg gccgttacgc gcccgggcag ggttccgtgg
      961 gggccgacgc catagcgtcc gacggcgccc tcgagcgtca ggcggcgcag cgccgctacc
     1021 ggatcgtagc ccttctctcc gcccggcccg gcgtggcgcg ggaagacgaa gccgtcgaac
     1081 ggcgagcgga acgagacggc gccgacgagc gcctcgttgg cggcaagcgc ttcgggtgct
     1141 ccggcgtacg cggcgccgaa cccggccgga tcgacgaggg tagcgccctc gggcgacaac
     1201 tcgtcgtcgc ggacctcgta gacggttcgc ccggagccta tggcgacgag cagg
//
LOCUS       DAOOLN010000772         1253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_146648_length_1253_cov_3.210351, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000772 DAOOLN010000000
VERSION     DAOOLN010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146648_length_1253_cov_3.210351"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="PLW80_09520"
     CDS             <1..984
                     /locus_tag="PLW80_09520"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB66792.1"
                     /translation="LSGIAYAADPQTPYAAMRSKPDQVDYIQYPYQTLAYRGGDSDDI
                     AALYAAELESIGVPAALVLLDDEVLVAFRMSGGESTTRGAFSDSGDFIFVDGEAWAPI
                     RVTMLREGFLRAWSEGSAAVRKSADARDRFYRLTDAWRRFPPAGVPGIASSTRKPSED
                     QVRAAFDNAVSLVVAKEVAPRAERMRLAFGPGGGSGRQHNSLGVLYARYGMYAEALTE
                     FQAAASLGFASAAVNIGNVAFLTGDYTTAAAWFDKAAKDRPEDVAAIIGLARSLYELD
                     RYDEADELFRKATNYMPELAERYGYLSARLTGSTARASAVMDRGGGMLWDE"
     gene            1021..>1253
                     /locus_tag="PLW80_09525"
     CDS             1021..>1253
                     /locus_tag="PLW80_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66793.1"
                     /translation="MKKRTGTGFLLAAFGSVAGVGAQGLPEKGAQALDDARIGTPLAD
                     RFGVSAYADGWFPRAIDDASAIGQTLGSLLLNCR"
BASE COUNT          187 a          428 c          446 g          192 t
ORIGIN      
        1 ctgtcgggca tcgcgtacgc cgccgacccg cagacgccct acgccgcgat gcgctcgaag
       61 ccggaccagg tcgactatat ccagtatccg taccagaccc tcgcctaccg cggcggcgac
      121 tccgacgaca tcgcggcgct gtacgccgcc gagctcgagt cgatcggcgt gccggccgcg
      181 ctcgtcctgc tcgacgacga ggtgctcgtc gcgttcagga tgtctggcgg cgagtcgacg
      241 accaggggcg cgttctccga ctcgggcgac ttcatcttcg tcgacggcga ggcctgggcg
      301 ccgatacgcg tgacgatgct gcgcgagggc ttcctgcgcg cctggagcga gggctcggcc
      361 gccgtcagga aatccgccga cgcccgcgat cgcttctacc ggctgacaga cgcctggcgg
      421 cgcttcccgc cggccggcgt gcccggcata gcctcgtcta cgaggaagcc gtccgaggac
      481 caggtccgcg ccgccttcga caacgcggta tcgctggtcg tggccaagga ggtcgcgccg
      541 cgcgccgagc gcatgcggct ggccttcggg ccgggcggcg gatcgggaag gcagcacaac
      601 tcgctcggcg tgctctacgc gcgctacggc atgtacgcgg aggcgctgac cgaattccaa
      661 gccgcggcct cgctcggctt cgcctcggcc gcggtcaaca tcggcaacgt cgcgttcctg
      721 accggcgact atacgaccgc ggcggcatgg ttcgacaagg ccgccaagga taggcccgag
      781 gacgtcgcgg ccatcatagg cctcgcgcga tccctctacg agctggaccg ctacgacgag
      841 gccgacgagc tgttccgcaa ggccacgaac tacatgcccg agctggccga gcgctacggc
      901 tacctgtcgg ccaggctgac cggctccacg gccagggcgt cggccgtgat ggacaggggc
      961 gggggcatgc tctgggatga atgagcccgg ggctaccgtc gggcgtaagg gtggatattc
     1021 gtgaaaaaac gtacggggac cggtttcctg ctcgccgcct tcgggagcgt cgcgggggtc
     1081 ggggctcagg gcctgcccga gaagggtgcg caagcactgg atgacgctcg gatcggtacg
     1141 ccgctggcgg atcgcttcgg cgtgtcggcc tacgccgacg gctggttccc gcgcgcgata
     1201 gacgacgcgt cggcgatcgg tcagacgctc ggatcgttgt tgctgaactg ccg
//
LOCUS       DAOOLN010000773         1253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_146660_length_1253_cov_3.097663, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000773 DAOOLN010000000
VERSION     DAOOLN010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146660_length_1253_cov_3.097663"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PLW80_09530"
     CDS             complement(<1..449)
                     /locus_tag="PLW80_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HPB66794.1"
                     /translation="MTYITGFHAMEEFVRSGKAGDATLLVAAAGPRIKKIIELADKAG
                     VGRKAVRREELDAAAPDHRGAAIALEVAPVAAEPSLEAWLETRRGPEAPARSVVVVLD
                     HIEDPHNLGAILRSADVFAADLVVVPERRAAKETDTVARSSAGAVAYV"
     gene            complement(483..1244)
                     /locus_tag="PLW80_09535"
     CDS             complement(483..1244)
                     /locus_tag="PLW80_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HPB66795.1"
                     /translation="MAAMTGYKAIDKEYIEARMTEKGISAETRAKYDEKKPGFWASLS
                     QDRDDYLHFLKTVLYEEVASGDCIVTGRGGFAIFAGVPGCISVKLVAPRDVRLQRIRT
                     RFQCDDKRAELLLRQNDRDRAGFHDYFFGVNWSDPVFYDLTINTGREHPATVARIIDQ
                     LRSLIVSQEAEKACLGRMAELRLGQAIITEIVYARRVPVHFVEADVKGSRVTLHGVAN
                     TQSAIDAASAAAGAVPGVETVDNAIQIVQEFAVMP"
BASE COUNT          174 a          424 c          440 g          215 t
ORIGIN      
        1 acgtacgcta ccgcgccggc cgacgagcgg gctaccgtat cggtctcctt ggccgcgcgg
       61 cgctccggta ccacgacgag gtcggcggcg aacacgtcgg ccgagcgcag gatggcgccg
      121 aggttgtgcg gatcctctat atggtcgagt acgacgacga cggaccgcgc cggcgcttcc
      181 ggcccgcgcc gggtctccag ccaggcctcc aggctgggtt cggcggcgac tggcgccacc
      241 tcgagcgcga tggccgcccc gcgatggtcc ggggcggccg cgtcgagttc ctctcgccgt
      301 acggccttgc ggccgacgcc ggccttgtcg gccagttcga tgatcttctt gatgcgcggc
      361 ccggccgccg cgacgagcag ggtggcgtcc cccgccttgc cggagcggac gaactcttcc
      421 atggcgtgaa agccggtgat gtacgtcatg cggcgatact atcccgagcg ggccgtccga
      481 gcctacggca tcaccgcgaa ttcctgcacg atctggatcg cgttatcgac cgtctcgacg
      541 ccgggtaccg cgccggcggc cgcgctggcc gcgtcgatcg cgctctgggt gttggcgacg
      601 ccgtgcagcg tcacccgcga gcccttgacg tccgcctcga cgaagtggac cggaacgcgc
      661 ctggcgtaga cgatctcggt gatgatggcc tgaccgaggc gcagctccgc catgcgcccc
      721 aggcaggcct tctcggcttc ctgggagacg atcagcgacc gcagctggtc gatgatgcgg
      781 gctaccgtgg ccgggtgctc cctgccggtg ttgatcgtca ggtcgtagaa gaccggatcg
      841 gaccagttga cgccgaagaa gtagtcgtgg aagccggcgc ggtcgcggtc gttctgcctg
      901 agcagcagct cggcgcgctt gtcgtcgcac tggaagcggg tgcgtatgcg ctggagccgg
      961 acgtcgcgcg gcgccacgag cttgaccgag atgcagcccg gtacgcccgc gaagatggcg
     1021 aagccgccgc gcccggtgac gatgcagtcg ccggaggcca cctcctcgta caggaccgtc
     1081 ttgaggaagt gcaggtagtc gtcgcggtcc tgggacagcg aggcccagaa gccgggcttc
     1141 ttctcgtcgt acttggcccg ggtctcggcg gagatgccct tctccgtcat ccgcgcctcg
     1201 atgtactcct tgtcgatagc cttgtagccg gtcatcgcgg ccaattcctt ggt
//
LOCUS       DAOOLN010000774         1253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_146671_length_1253_cov_2.889816, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000774 DAOOLN010000000
VERSION     DAOOLN010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146671_length_1253_cov_2.889816"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..>1253)
                     /locus_tag="PLW80_09540"
     CDS             complement(152..>1253)
                     /locus_tag="PLW80_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66796.1"
                     /translation="MVTVYVLAQYVTADLNKEKARIAAVFKEKVDAVAKPEAAGDSAV
                     TAGRYFDAVKSYVEAVVAASGSDIDNAAIKLERNANKARAVLAKLRFVAVDAPTAAGL
                     GKDYAKPFKARLVFGEGDSAPGIPGAEVYVAYQRRQSSGRVVTRTERAMTDADGVVSF
                     APPPPDFVGKASLRFSLNLDSTSELLERVPAAYDAYVAAIADELARRSVTFDYVISSA
                     AKTVPTGVVIVDLGDDGQPLGTTIAQGGLFETLAKEKFKAGLAPLSAAMIASFDDAAI
                     LKAAKAAYGAGLSRLIYGAARIERSEKDGSMWQATARMTVRCVDFATGAILYSGEKTA
                     VSVAADEASAKRSALLQVARDGVAKDLMANLP"
BASE COUNT          210 a          394 c          413 g          236 t
ORIGIN      
        1 gccgaggcga cgaagaggaa aaaggatacg agatacgaaa gaggaagtct gaagaaagag
       61 ctttaaggtt tttggtcctt ttaaagcttt tccctttctc ttctcttatt ctcgtatcct
      121 cttcgccgcc ttaggcgtat agatctatcg tttacggcag attggccatc agatccttgg
      181 ccacgccgtc cctagccacc tggagcaggg ccgagcgttt tgcactcgcc tcgtcggcgg
      241 cgaccgagac ggcggtcttc tcgccgctat acaggatggc cccggtggcg aagtcgacac
      301 agcgcaccgt catcctcgcg gtagcctgcc acatcgagcc gtccttctcg gagcgctcta
      361 tcctagccgc gccgtagatc agcctagaga gtccggcccc gtacgcggcc ttggccgcct
      421 tgaggatggc cgcatcgtcg aaggacgcga tcatcgcggc gctgagcggg gctaagcctg
      481 ccttgaactt ttccttggcc agagtctcga agagaccgcc ctgggcaatg gtcgtgccga
      541 ggggctggcc gtcgtcgccg aggtccacga tcacgacgcc ggtcggcacc gtcttggcgg
      601 cagacgatat gacgtagtcg aaggtcacgg agcgtcgggc cagctcgtcc gctatggccg
      661 cgacgtaggc gtcataggcc gcgggcacgc gttcgagcag ctcgctcgtc gaatcaaggt
      721 tcagcgaaaa gcgcaggctc gccttgccga cgaagtcggg cggcggcggg gcgaaggaca
      781 ccacgccatc ggcgtcggtc atggcgcgct cggtcctggt cacgacgcgt ccggagctct
      841 gccttcgctg gtaggcgacg tacacctcgg cgccaggtat gcccggggcg gagtcgccct
      901 cgccgaagac gagcctggcc ttgaacggct tcgcgtagtc cttgccgaga ccggccgcgg
      961 tcggcgcgtc gacggcgacg aagcgcagct tggcgagcac ggcgcgcgcc ttgttggcgt
     1021 tgcgctccag cttgatggcg gcgttatcga tatcggagcc ggaggcagcg acgacggcct
     1081 cgacgtagct cttgaccgcg tcgaaatagc gcccggccgt aaccgcggag tctccggccg
     1141 cctcgggctt ggcgacagcg tcgaccttct ccttgaagac ggccgcgatg cgggccttct
     1201 ccttgttcag gtcggcggta acgtattggg cgagcacgta gacggtgacc atc
//
LOCUS       DAOOLN010000775         1252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_146859_length_1252_cov_2.908104, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000775 DAOOLN010000000
VERSION     DAOOLN010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146859_length_1252_cov_2.908104"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PLW80_09545"
     CDS             <1..792
                     /locus_tag="PLW80_09545"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HPB66797.1"
                     /translation="VLHGTFGEDGTVQGLLECAELPYVGADVAGSAIAMDKELAKSLW
                     LQAGLPVVPSITARPADADKVDELAERIESAFGWPAFVKPARCGSSVGASKISGPADL
                     QGAVRSALLYDTKAVIEPFIQAREIECAVLGNDEPRAFPPGEIVPSHEFYDYDAKYID
                     PDGAALVIPAAIDEATAARVMDIAVAAYKAASLSGMARVDFFIDKKDGSVRLNEANSI
                     PGFTSISMYPMMCEAGGLSYSALLDELLELAVDKKRQRDALRFSR"
     gene            931..>1252
                     /locus_tag="PLW80_09550"
     CDS             931..>1252
                     /locus_tag="PLW80_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPB66798.1"
                     /translation="MKSGDYIEMDEKYGAHNYHPLPVVISWALGTKVKDPDGKEYYDF
                     LSAYSAVNQGHRHPRIIKALVDQANVCTLTSRAFHNDRMGLFLKKLCEFTGYDMALPM
                     NTGVE"
BASE COUNT          244 a          398 c          379 g          231 t
ORIGIN      
        1 gtgctacacg gcaccttcgg cgaggacggc accgtgcagg gactcctcga gtgcgccgag
       61 ctaccgtacg tcggcgccga cgtggctggc tcggccatag ccatggataa ggagctggcc
      121 aagtcgctat ggctccaggc cggacttccg gtcgtgcctt cgatcaccgc tcggccggcc
      181 gatgcggaca aggtcgacga gctggctgag cgcatcgagt cggccttcgg ctggcctgcc
      241 ttcgtgaagc cggcgcgctg cggctcgagc gtcggcgcgt cgaagataag cggcccggcc
      301 gatctccagg gcgccgtccg ctcggccctg ctctacgata ccaaggccgt gatcgagccg
      361 ttcatccagg cgcgggagat cgagtgcgcc gtactcggca acgacgagcc cagggccttc
      421 cctcccggcg agatcgtgcc gtcgcacgag ttctacgatt acgacgccaa gtatatcgac
      481 ccggacggcg ccgccctcgt catcccggcc gcgatagacg aggcgacggc cgctcgcgtg
      541 atggacatag ccgtggccgc ctacaaggcg gccagcctct ccggcatggc cagggtagac
      601 ttcttcatcg acaagaagga cggctcggta cgcctcaacg aggcgaactc cattcccggc
      661 ttcacctcca tctcgatgta ccccatgatg tgcgaggccg ggggcctgtc ctattctgcc
      721 ttgctcgacg aactcctcga gcttgccgtc gacaagaagc gccagcggga cgcgctcagg
      781 ttctcgcgtt gatatttatg cacttcatac gaataattat tgatattgca tatagttaaa
      841 agctcggata cgatgattcg tctggaaggc gtcgtaagcg cgtatcgtaa cgcctcggcc
      901 gacgcgaggc tctttcgtag gaggtatcga atgaagagcg gcgattacat cgaaatggac
      961 gagaagtacg gggctcataa ctaccaccca ctacccgtgg tcatctcatg ggccctcggt
     1021 accaaggtca aggacccgga tggcaaggaa tactacgatt tcctctcggc ctactccgcg
     1081 gtcaaccagg gccaccgcca cccgaggatc atcaaggccc tcgtcgatca ggccaatgtc
     1141 tgcacgctga cgagccgagc cttccataac gacaggatgg gcctgttcct gaagaagctg
     1201 tgcgagttca ccggctacga tatggccctg ccgatgaaca ccggtgtcga gg
//
LOCUS       DAOOLN010000776         1252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_146921_length_1252_cov_2.257310, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000776 DAOOLN010000000
VERSION     DAOOLN010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146921_length_1252_cov_2.257310"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(346..564)
                     /gene="csrA"
                     /locus_tag="PLW80_09555"
     CDS             complement(346..564)
                     /gene="csrA"
                     /locus_tag="PLW80_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HPB66799.1"
                     /translation="MLILSRKINEKVVIGHGVVVSIVEIRGDQVKLGIEAPRSVKVFR
                     QEVYDAIQDENRLAAGSAAELPALELPQ"
     gene            complement(569..1009)
                     /locus_tag="PLW80_09560"
     CDS             complement(569..1009)
                     /locus_tag="PLW80_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HPB66800.1"
                     /translation="MKIQTKILGELDVDERQVIDIPDGLIGFKKFTKYALLDAPQKPY
                     FFLQSTELAELCFILLDPFLFRPDYSVDAADEAMSALGVTGPDDALVLSLVTVPPDGG
                     PVTANLMGPLVIGRKSRRGAQVVLADPRWRTKHDVMAELAASRK"
     gene            complement(1032..>1252)
                     /locus_tag="PLW80_09565"
     CDS             complement(1032..>1252)
                     /locus_tag="PLW80_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgL"
                     /protein_id="HPB66801.1"
                     /translation="GAVALGGSGPGLDMAALRLNREIPDVTKLLAAEKDLDMTQAITD
                     FKMMEYARNATLQMAGRVLPKSLLDFLR"
BASE COUNT          201 a          403 c          416 g          232 t
ORIGIN      
        1 gattctcggt tcaactcgga actgaatacg cttgaatgag ttgttcagga aaccctacct
       61 actaacatca acgacgcgcc gccccgagaa ccttgttcgt acaaaggcct cgcatacacg
      121 cggaggcgag tgtccaggag acggcgcccc tcgcgtagcg aggggatcgc ctcctgggca
      181 ggccctgccg agatgatcgc gatcgccgac tcatcgcggc catcgccggc catcgcttaa
      241 cctgactcgc ttccgctcgc aggttaacgg cggcgaggcc cgtattcatt ctcgaacccg
      301 gctcggggcg gcgcgtcgtg tgatactcgc ggcgcgtcgg gttacttact ggggaagctc
      361 cagggccggc agctcggcgg cggagccggc ggccaggcgg ttctcgtcct ggatggcgtc
      421 gtagacttcc tgcctgaaga ctttgacgct cctcggggcc tcgatgccga gcttcacctg
      481 gtcgccccgg atctcgacga tcgacacgac cacgccgtgt ccgatcacga ccttctcgtt
      541 gatcttgcgc gatagtatga gcatcgcgct acttcctcga ggcggccagc tccgccatca
      601 cgtcgtgctt ggtgcgccag cgcgggtcgg cgagcaccac ctgggcgccg cgcctgctct
      661 tgcggccgat gacgaggggg cccatcaggt tggcggtgac ggggccgccg tccggcggca
      721 ccgtgacgag ggacagcacg agcgcgtcgt cggggccggt cacgccgagc gccgacatcg
      781 cctcgtccgc cgcgtcgacc gagtaatcgg gcctgaacag gaacgggtcg agcaggatga
      841 agcacagctc ggccagctcg gtcgactgca ggaagaagta cggcttctgg ggcgcgtcga
      901 gcagcgcgta cttggtgaat ttcttgaagc cgatgaggcc gtcggggatg tctatcacct
      961 gtcgctcgtc gacgtccagc tcgccgagga tcttggtttg tatcttcatg atgccgccaa
     1021 atctgccgga ttcatcgcag gaagtcaagg agcgacttgg gcagcacgcg gccggccatc
     1081 tggagcgtcg cgttgcgggc gtattccatc atcttgaagt cggtgatggc ctgggtcatg
     1141 tcgaggtcct tctccgcggc caggagcttg gtcacgtcgg gtatctcgcg gttcagccgc
     1201 agggccgcca tgtccaggcc cgggccgctg ccgccgagcg ccacggcgcc ga
//
LOCUS       DAOOLN010000777         1251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_147063_length_1251_cov_2.734950, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000777 DAOOLN010000000
VERSION     DAOOLN010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147063_length_1251_cov_2.734950"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLW80_09570"
     CDS             <1..208
                     /locus_tag="PLW80_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPB66802.1"
                     /translation="AVLLSWGIKRVNAIQGSIITGLEPVFNPLWVFLALGEKPSPNAL
                     AGGAVIIAAVVGSSIVTARRKTKA"
     gene            523..1047
                     /locus_tag="PLW80_09575"
     CDS             523..1047
                     /locus_tag="PLW80_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66803.1"
                     /translation="MKITRKRAPIIAVAIAALAVSAYAEGSGHGASAAVEAGRAITIV
                     DRLRWAVEDEYLARGEYEAVMARFGAMRPYDNIMAAEEQHLAWLKAEYQARGLPFPTD
                     GSASSVVVPAELKAAAQAGVDAEIANIAMYSSFLSRPELSRPENASVRDLFERLKRAS
                     ENHLRAFKTQLSKH"
BASE COUNT          253 a          397 c          368 g          233 t
ORIGIN      
        1 cgcggtgctc ctgtcctggg gtatcaagcg ggtcaacgcc atccagggct ccatcatcac
       61 ggggctcgag ccggtgttca acccactctg ggtgttcctg gccctcggcg agaagccgag
      121 cccgaacgcg ctcgccggag gcgcggtgat catcgcggcc gtcgtcggct catccatcgt
      181 cacggcgcgc aggaagacca aggcatagcg gccgagcgca tcgcgtcttc tccgctccgc
      241 caccgtaccc cgattcgcct tacgccaaag taccccaagc cgtcgtcgcc ttctggcgtt
      301 caggcacgtg catttttttc acatcgccta acgtttcttt ctcgatcgga tgaatactta
      361 gtcgcacatt ccaccgcccg ccaggtacag cctggtgggc aacatcgttt tcatattgta
      421 tatcattata taataatgac ataaaatcag ttcatacaaa tctaatcaac ctggcacact
      481 tactgctata tagtatttac tctatgcctg gtgggggtgc ctatgaaaat cactagaaaa
      541 cgcgcgccca taatcgccgt cgcgatcgca gcgctggccg tatcggccta cgccgagggt
      601 tcgggccacg gggcgagcgc cgcggtcgag gctggccggg caatcactat cgtcgacagg
      661 ctccgttggg cggtcgagga cgagtacctg gctcgcggcg aatacgaggc tgtcatggcc
      721 aggttcggcg ccatgaggcc gtacgacaac atcatggcgg ccgaggaaca gcatctcgcc
      781 tggctcaagg ccgagtacca ggcccgcggc ctacccttcc ccacggacgg ctcggcctcg
      841 agcgtcgtcg ttcctgcgga actgaaagcc gccgcccagg ccggcgtcga cgcggagatc
      901 gccaacatag cgatgtactc gtcctttctg agccgccccg agctatcgcg ccccgagaac
      961 gcgagcgtca gggacctgtt cgagcggctg aagcgcgcga gcgagaatca cctgcgggcc
     1021 tttaaaacgc agctgtccaa gcactagact agaaacgatc aggagcaagg aggcaccatg
     1081 gcaagcggcg cgtcgaccaa aaagccgaag aaggcatcgc gatggatcag gcgggcggtt
     1141 cagatcctgt tcttcgccct ggtcgcgacc atagcgacga atgagatact gagcgagcga
     1201 ggcatcacgg cgctgtcgtt cgtcgagggc gcctcgctgc acgcgatctg c
//
LOCUS       DAOOLN010000778         1250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_147201_length_1250_cov_3.906276, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000778 DAOOLN010000000
VERSION     DAOOLN010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147201_length_1250_cov_3.906276"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1250
                     /locus_tag="PLW80_09580"
     CDS             <1..>1250
                     /locus_tag="PLW80_09580"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPB66804.1"
                     /translation="EPAIRERVHARFRVIMVDEYQDSNVAQFELLKALAGPEAYVCVV
                     GDDDQSIYRFRGAEVRNILSFPEVFPRTAVVKLERNYRSYQSILDIASRVVENNEGRL
                     GKTLRATRTGGEKPTLAVLRDHDEETAYCIRVIDAHLRTGGSFSDVAILYRTNAQSLA
                     FERAFPPAGIPYRLVGALRFYEREEVKDALALLALASNPRDEVAFRRVANKPARGVGD
                     ASLEAVVEAAFLGDGDLVAAAAEARAKSAKARAGLAAFAALMQKLAERLAAPALADDG
                     GRALDDDAPGPSGPPPVAPGAERLSAVVEYAVRESGLLQYHKDQDDIAGSQKTANLDE
                     LVNAASDYPATREGLAAFLEAVELDRAMSSGEVSQDAVTLITMHNTKGLEFPVVVIAG
                     MEQGLFPRDDDEGARLPELLDVGQ"
BASE COUNT          206 a          448 c          421 g          175 t
ORIGIN      
        1 cgagccggcg atacgcgagc gcgtccacgc gcgcttccgc gtgatcatgg tcgatgagta
       61 ccaggactcg aacgtggccc agttcgagct tctcaaggcg ctcgccggtc ccgaggccta
      121 tgtctgcgtc gtcggcgacg acgaccagag catctatcgc ttccgaggag ccgaggtcag
      181 gaacatcctg agcttccccg aggtcttccc gcgcacggcc gtcgtcaagc tcgaacgcaa
      241 ctaccgctcc taccagagca tcctcgacat agccagccgc gtcgtcgaga acaacgaagg
      301 ccgcctcggg aagacgctcc gcgcgacgag gacgggcgga gagaagccga ccctcgcggt
      361 gctccgcgat cacgacgagg agaccgccta ctgcatccgc gtgatagacg cccacctgcg
      421 tacaggcggc tcgttctccg acgtggccat cctataccgt acgaatgccc agtccctcgc
      481 cttcgagcgg gccttcccgc cggccggcat cccataccgc ctggttggcg ccttgcgctt
      541 ctacgagcgt gaggaggtca aggacgcgct cgcgctcctg gccctggcgt cgaacccacg
      601 ggacgaggtg gccttcaggc gcgtcgccaa caagccggcg aggggcgtcg gcgacgcgag
      661 cctcgaggcc gtcgtcgagg cggcgttcct tggcgacggc gacctcgtcg cggcggcagc
      721 ggaggcccgg gccaagtcgg ccaaggcccg cgcggggctc gccgccttcg ccgccctgat
      781 gcagaaactc gccgagcggc tcgccgcgcc agccctggcc gacgatggcg ggcgggcctt
      841 ggacgacgac gcgccgggtc cgagcggccc tccgcccgtg gcgccaggtg ccgagcggct
      901 gtccgccgtc gtcgaatacg cggtgcgcga gtcggggctc ctccagtacc acaaggacca
      961 ggacgacata gccggctccc agaagacggc caaccttgac gagctggtca acgccgcgag
     1021 cgactacccg gcgacccggg aaggacttgc ggccttcctc gaggcggtcg agctcgatcg
     1081 cgccatgtcg tcgggagagg tatcgcagga cgccgtcacc ctcataacga tgcacaacac
     1141 caagggcctc gagttcccgg tggtcgtgat cgccggcatg gagcaggggc tcttcccccg
     1201 cgacgacgac gagggcgcgc gccttccgga actgctcgac gtgggccagg
//
LOCUS       DAOOLN010000779         1249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_147410_length_1249_cov_3.267169, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000779 DAOOLN010000000
VERSION     DAOOLN010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147410_length_1249_cov_3.267169"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..1015
                     /gene="hflK"
                     /locus_tag="PLW80_09585"
     CDS             44..1015
                     /gene="hflK"
                     /locus_tag="PLW80_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HPB66805.1"
                     /translation="MPERNVTPIRGLKVGTIVLIVIAVAVIALFSTSFFVVDQTEQAV
                     VLTLGKFSGIAEPGLHFKWPFGLQRNYNVKTKVVQTEQFGFRTAQLGVQARYEDRTYP
                     EESTMLTGDLNIVDVEWIIQYRITDAKAWLFNLQDKQKTIRDISQSAINMLVGDMTIL
                     DVMGSQRVAIEQAGQQLMNETLKGYGLGIEIIAVKLQNIVPPVGVQAAFEDVNKAIQD
                     MNRLISEGKEAYNKEIPKARGEADRIVQIAKGYAAERVNKANGDVARFSSVYEEYRKA
                     PDVTRDRLYYEMIEAVFADERNVELIDSELSNFIPIKNFGTQSGGTK"
     gene            1012..>1249
                     /locus_tag="PLW80_09590"
     CDS             1012..>1249
                     /locus_tag="PLW80_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HPB66806.1"
                     /translation="MKKLITVLVVAVVLLGLLVLMGPFYIQQEGFQSVIVRFGQITRV
                     ETEAGLKLKTPFIDNVVTYPKKILMWDGEAQRIPT"
BASE COUNT          289 a          396 c          358 g          206 t
ORIGIN      
        1 aaccaaggcg acggcggtac cctaaaccat cataggaggt tccatgcctg aaaggaacgt
       61 tacaccgatc cgcggcctga aggtcggcac gatcgtgcta atcgtcatcg cggtcgcggt
      121 catagcgcta ttctccacga gcttcttcgt cgtcgaccag accgagcagg cggtcgtgct
      181 gacgctcggg aaattcagcg gcatcgccga gccgggactg cacttcaaat ggccgttcgg
      241 cctgcagcgc aactataacg tcaagaccaa ggtagtccag accgaacagt tcggcttccg
      301 cacggcccag ctgggcgtcc aggcgaggta cgaggaccgg acctaccccg aggaatccac
      361 catgctgacc ggcgacctca acatcgtcga cgtcgagtgg atcatccagt accgcatcac
      421 tgacgccaag gcctggctgt tcaacctcca ggacaagcag aagaccatac gcgacatcag
      481 ccagtcggcc atcaacatgc ttgtcggcga catgaccatc ctcgacgtga tgggatcgca
      541 gcgcgtggcc atcgagcagg ccggccagca actgatgaac gagaccctga agggctacgg
      601 cttgggcatc gagatcatag ccgtcaagct ccagaacatc gtgccgccgg tcggcgtcca
      661 ggccgccttc gaggacgtca acaaggccat ccaggacatg aaccgcctga tctccgaagg
      721 caaggaagcc tataacaagg agatcccgaa ggcccgaggc gaggccgacc gtatcgtcca
      781 gatagccaaa ggctacgcgg ctgagcgcgt caacaaagcc aacggcgacg tggcccgctt
      841 cagctccgtc tacgaggagt accggaaggc gcccgatgtg actcgggatc gtctgtacta
      901 cgagatgatc gaggcggtgt tcgccgacga gcggaacgtc gagctgatcg acagtgagct
      961 cagcaacttc ataccgataa agaacttcgg tacccagtcc ggaggcacca agtgaaaaaa
     1021 ctcataaccg tcctggtcgt ggccgtcgtc ctgctcggcc tcctcgtctt gatgggcccc
     1081 ttctatatcc agcaggaggg cttccagtcg gtcatcgtcc gcttcggcca gatcaccagg
     1141 gtcgagaccg aggccggcct caagctcaag actccgttca tcgacaacgt ggtcacctac
     1201 cccaagaaga tcctcatgtg ggacggcgag gcccagcgta tcccgacca
//
LOCUS       DAOOLN010000780         1248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_147589_length_1248_cov_3.440905, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000780 DAOOLN010000000
VERSION     DAOOLN010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147589_length_1248_cov_3.440905"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PLW80_09595"
     CDS             <1..346
                     /locus_tag="PLW80_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66807.1"
                     /translation="VCPPDARDARITISQIHTHKIRGADIVLFAEKHPELDLAVKGVP
                     SGRDDYRSAYVEIPASGDRYLFVFAATVALQYLAFRMSVLKMEYLDRLGVRDHGVHPD
                     APKNVSKSITVD"
BASE COUNT          195 a          445 c          410 g          198 t
ORIGIN      
        1 cgtctgcccc ccggacgccc gcgacgcgcg catcaccatc agccagatcc acacccataa
       61 gatccgcggc gccgacatcg tgctgttcgc ggagaagcat cccgagctcg acctggccgt
      121 caagggcgtt ccctccggcc gcgacgacta ccgctcggct tacgtggaga tacccgcctc
      181 gggcgaccgg tatctcttcg tgttcgccgc caccgtcgcc ctccagtacc tggccttcag
      241 gatgtcggtg ctcaagatgg agtacctcga ccgcctcggc gtacgggacc acggcgtcca
      301 cccggacgcg cccaagaacg tgtccaaatc catcaccgtc gattaagcgc gcgaggtcga
      361 cgatacacct cgcatacggc cgggcccttt cgggcccggc cgtatgcgta gcgcggcgct
      421 ggctatcgag tgggcgcccg ggacgggctc gcgggggccg gaggccggag cggcggctcc
      481 gagccggcgt gaaacgaggc gacaggcggg ctatagcggc cgtcaggacg ggcctgagcg
      541 ccgcgaccgc gggtttcagg aacctgaagt tagccgtgaa cacctcgttc cactgatcga
      601 aggtatggtt ctggtagccg aggatggact ggacttccgc ggtgtcgtac cagtccaggt
      661 aatacgatcc caggttgtcg gggaagagct cctcgcgcgg catggcgagc cccatagatc
      721 cgaaggctcc ttcgagcatg gcctggatgc gtatctggca tccgttcgcg gcgccgccgg
      781 ctatgaagcc ggtcctaccc cgcatctctc ccgcgccgcg gaggttctcg accgccgaga
      841 ccagggtgga agccgcgtcg gcgtcgtaga cgatctcgca gcgcgccgcg agcggcatgt
      901 cgtagataat ccgtatcatg cccaggacgg tgcccaggcc gacgtagctc ggcatcaccg
      961 ccgcggcctt gatcctcgtg tacgcgtcga ccgggttggc cgggtcgccg atacggaccc
     1021 ggacgccgac gcggccgtac cgccgcccca gccgctccgc gcgcggccag gcactcgtcg
     1081 gtgacgcgca tgccgacatt gccgaatccg cccgtcacga gtatgcgcat ggtcggctct
     1141 ccttatcggc cggcctgtgg cgcgccggcg cgcttcgatt ccccctacat tctaggatac
     1201 cggtcggccc cgcgccaggg cggcccggta cgacgcggcc agcgccga
//
LOCUS       DAOOLN010000781         1247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_147866_length_1247_cov_2.067953, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000781 DAOOLN010000000
VERSION     DAOOLN010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147866_length_1247_cov_2.067953"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PLW80_09600"
     CDS             complement(<1..839)
                     /locus_tag="PLW80_09600"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPB66808.1"
                     /translation="MSFPVETVRHSLSHVMAEAVVKLYPGAKVAIGPAIDDGFYYDFD
                     LPSPIQPDDLPAVEKEMRRIISANVPFKRVEVSKAEARDLFKDEPYKLELIDGLDDGT
                     ITVYEQGAFRDLCRGPHVDSTRDLRPDCFKLRAVAGAYWRGDEKRPMLTRIYAYAFAS
                     KAELEAHLKMLEEAEKRDNRKLGKELDLFSTHEEAGAGLVYWHPKGGRFRVELENWWR
                     DEHYKNGYEILFTPHIGKSWLWETSGHLGFYKEGMYSPMEIDEDDYYIKPMNCPFHMM
                     IYK"
BASE COUNT          190 a          400 c          416 g          241 t
ORIGIN      
        1 ttcttgtaga tcatcatgtg gaaggggcag ttcatcggct tgatgtagta gtcatcctcg
       61 tcgatctcca tcggcgaata catgccttcc ttgtagaagc cgaggtggcc agaggtctcc
      121 cagagccagc tcttgccgat gtgcggggtg aagaggatct cgtagccgtt cttgtagtgc
      181 tcgtcgcgcc accagttctc gagctcgacg cggaagcggc cgcccttcgg gtgccagtag
      241 acgaggccgg cgccggcctc ctcgtgggtg ctgaagaggt cgagctcctt gccgagcttg
      301 cggttgtcgc gtttctcggc ttcctcgagc atcttcaggt gggcctcgag ctcggccttg
      361 ctggcgaagg cgtaggcgta gatgcgggtg agcatcggcc gcttctcatc gccgcgccag
      421 tacgcgccgg cgaccgcgcg tagcttgaag cagtcgggcc tgaggtcgcg ggtcgagtcg
      481 acgtggggcc cgcggcagag gtcgcggaag gcgccctgct cgtagacggt gatggtgccg
      541 tcgtcgagcc cgtcgatcag ctcgagctta tacggctcat ccttgaacag atccctggcc
      601 tcggccttgg atacctccac gcgcttgaac gggacgttcg ccgagatgat gcggcgcatc
      661 tccttctcga cggctggcag gtcgtccggc tggatgggcg acggcaggtc gaagtcgtag
      721 tagaagccat cgtcgatcgc gggcccgatg gccaccttgg cgcctgggta cagcttgacg
      781 acggcctcgg ccatcacgtg ggacagggaa tggcgaacgg tctctacggg aaagctcata
      841 cggtactcct cggacgggac cgctcgggcg ggtcgcgtcc atacgcacgc ccgctcggga
      901 cggactcccg gcgttccagg acgcgtatcg cgtccttagc gtaaggacga accccgctca
      961 agccccctgc gaggcccgcc gcccagcgac gacgtcgccg tacggctcgc ggaatccgcg
     1021 gtaccacctt acttcggtcc gcccgcatgg gccgaccgcg ctctagcagc tattacgggc
     1081 acgcccggct cccaatacag gccggcatat gtcggcgttc acgggagcgg ctccggagcg
     1141 gttttcggcg ggatcgcgtc gggcgcctct cagcctgggg cgccctctct cttggcgcga
     1201 cggcccgcgt actcgtctcc atcaaagcct gtgaccatga atatgct
//
LOCUS       DAOOLN010000782         1246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_147983_length_1246_cov_2.713686, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000782 DAOOLN010000000
VERSION     DAOOLN010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147983_length_1246_cov_2.713686"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLW80_09605"
     CDS             <1..208
                     /locus_tag="PLW80_09605"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HPB66809.1"
                     /translation="DGPRGISITGLASTKMACLDEPYGLSEYEFFAALSVATGWDLAN
                     GSLTLGGRAADGSSVELAFSSPGR"
     gene            227..>1246
                     /locus_tag="PLW80_09610"
     CDS             227..>1246
                     /locus_tag="PLW80_09610"
                     /inference="COORDINATES: protein motif:HMM:NF028509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPB66810.1"
                     /translation="MKFVTKHFYTVFVASMAILCAASAGTAFWAVRRAEGMNAALVQE
                     RIYEFKRTFLRDTVQNMIRDIDRLRDYNLRRAREDLDPLTDEAWADRETKATIADVIR
                     AKSFEHDGYIWVNEVLDWSGGDGYAVRRVHPNLVDTEGCLLSTKATDIKGNTPYLTEL
                     EGVREHGELFYRYYFKRMGSDEVSEKLTYAALYPDYDWIVAMGMHVDDIETYTLAVRE
                     AASDFNARVIALVVVLLVLFFGLGVALLIVAGRRFMDASARAIRTEANIDPLTGALNR
                     RIGDRYFSDAFRAFRYGKPSPLVLSIDLDDFKRVNDAYGHDAGDVVLRAVVERVKQTM
                     RASDFL"
BASE COUNT          219 a          415 c          395 g          217 t
ORIGIN      
        1 ggacggccca cgcggcatat cgataacggg cctcgccagc accaagatgg cctgtctcga
       61 cgaaccgtac ggcctctccg agtacgaatt cttcgcggcg ctgtcggtcg ccacgggctg
      121 ggatctggct aacggatcgc tcacgctcgg cggacgggcc gccgacggct cgtcagtcga
      181 gctggccttc tcgtcgcccg ggcgatagcc aggcgaccga cgcccgatga aattcgtcac
      241 gaagcatttc tatacggtat tcgtcgcctc gatggccata ctctgcgcgg cgagcgcagg
      301 aaccgccttc tgggcggtcc gccgcgccga gggcatgaac gcggccttgg tccaggagcg
      361 catctacgag ttcaagcgta cgttcctgcg ggatacggtg cagaacatga tacgcgatat
      421 cgaccgcctg agagactaca acctgcggcg cgcccgggag gatctggatc cgctgacgga
      481 cgaggcctgg gccgaccgcg agaccaaggc gacgatagcc gacgtcatcc gcgccaagtc
      541 gttcgagcac gacggctata tctgggtcaa cgaggtgctc gactggtcgg gcggcgacgg
      601 ctacgccgtc aggcgcgtcc acccgaacct cgtcgatacc gagggctgcc tcctgtcgac
      661 gaaggccacc gatatcaagg gcaacacccc gtacctgacc gagcttgagg gcgtcaggga
      721 gcatggcgag ctgttctacc gatactactt caagcgcatg ggtagcgacg aggtatccga
      781 gaagctgacc tacgcggcgc tctatcccga ctacgactgg atcgtggcta tgggcatgca
      841 cgtcgacgat atcgagacct ataccctggc cgtgcgtgag gccgcctcgg acttcaacgc
      901 tcgcgtgatt gcgctggtcg tcgtcctgct cgtgctgttc tttggcctcg gcgtggccct
      961 gctcatcgtg gcgggccgca ggttcatgga cgcctcggcc cgggcgatac gcacggaggc
     1021 caatatcgat ccgctgaccg gcgccctgaa ccgccgcata ggcgaccgct acttctccga
     1081 cgccttcagg gccttccgct acggcaagcc gtcgcccctc gtcctgtcca tcgacctgga
     1141 cgacttcaag cgcgtcaacg acgcctacgg ccatgacgcg ggcgacgtcg tgctccgagc
     1201 cgtcgtcgag cgcgtcaagc agacgatgcg cgcgagcgat ttcctg
//
LOCUS       DAOOLN010000783         1246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_147996_length_1246_cov_2.498741, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000783 DAOOLN010000000
VERSION     DAOOLN010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147996_length_1246_cov_2.498741"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="PLW80_09615"
     CDS             <1..774
                     /locus_tag="PLW80_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB66811.1"
                     /translation="LRLDLGEARFDRTAYPENSREVCRIILERLPHTLALFTLSTTIS
                     IVVGIALGTAMARKPGGRLDRASSLTAMILYGAPSWWIGLFMILLLGYTFPVFKVGAL
                     HSPEVAPGRLAWALDYLAYLALPLITLVLIKLFSFAYLTRAMVILPLHEDYVFAARGR
                     GLSEGRVVSRHGLRMAAPGLTTMAAQTFAQSIVGDILVEKIMMRPGLGLTLFSAISSN
                     DTALFTGVLAMVTAVYCLSFLFLDIAYAWLDPRIAYGRE"
     gene            935..>1246
                     /locus_tag="PLW80_09620"
     CDS             935..>1246
                     /locus_tag="PLW80_09620"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HPB66812.1"
                     /translation="MASFERTNPRFALCGLNCCLCPRYTTEGASRCPGCGGPGFSDIH
                     PTCAVLTCNKKRDDVEFCFRCGSYPCRKYLEQGAYDSFISYRRVQHNLDEAAADLAAY
                     EA"
BASE COUNT          197 a          410 c          398 g          241 t
ORIGIN      
        1 ctgagactgg atctcggcga ggcccgattc gaccggacgg cgtatccgga gaattcccgg
       61 gaggtctgtc gcatcatctt ggagcggctg ccgcatacgc tggccctgtt cacgctgagt
      121 acgacgatca gcatcgtcgt tggcatcgcc ctgggcacgg cgatggccag gaagccgggc
      181 gggcggctcg accgggcctc gagcctgacc gcgatgatac tctacggggc gccgtcctgg
      241 tggataggct tgttcatgat cctgctcctc ggctatacgt tcccggtctt caaggtcggt
      301 gcgctccatt ctcccgaggt cgcgcccggc cggctggctt gggcgctcga ctacctcgca
      361 tacctggcct tgccgcttat cacgctcgtg ctgatcaagc tgttcagctt cgcctacctg
      421 acgcgggcca tggtcatcct gccgttgcac gaggattacg tcttcgccgc tcgcggtagg
      481 ggcctgtccg agggccgggt cgtgagccgg cacgggctac gcatggccgc tccgggcttg
      541 acgacgatgg ccgcgcagac cttcgcgcag tccatcgtcg gcgacatcct cgtcgaaaaa
      601 atcatgatgc gtccggggct cggcctgacc ctattctcgg ccatatcgtc gaacgatacg
      661 gccttgttca ccggcgtcct ggcgatggtc accgccgtct actgcctcag tttcctgttt
      721 ctcgatatag cgtacgcctg gctggacccg cgcatcgcgt acggcaggga atagctcagg
      781 atcccgcgcc gcctattgac agcccggcct ccttgcagca aggtcgacga gggactgggg
      841 cgacgggccg gggcgacaca tcgaggcgac aagtcggggc gacaggtcga ggcgacgggc
      901 cgagtacacc gtctgcggtt tccgaggagg gagcatggcg agcttcgaac gaacgaatcc
      961 gcgcttcgcg ctctgcggcc tcaactgctg tctctgcccg cgctacacta ccgaaggcgc
     1021 gtcgcgctgc ccgggttgcg gtggtccagg cttttccgat atccacccga cctgcgccgt
     1081 cctcacctgc aacaagaagc gcgacgacgt ggaattctgc ttccgatgcg gctcgtaccc
     1141 ctgccgtaag tacctggaac agggggcgta cgactcgttc atatcgtata ggcgggtaca
     1201 gcataacctc gacgaagccg cggccgacct ggccgcgtac gaggcc
//
LOCUS       DAOOLN010000784         1245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_148174_length_1245_cov_2.173109, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000784 DAOOLN010000000
VERSION     DAOOLN010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148174_length_1245_cov_2.173109"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..886
                     /locus_tag="PLW80_09625"
     CDS             182..886
                     /locus_tag="PLW80_09625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPB66813.1"
                     /translation="MRLGIQKTSLVDFPGRVAAVLFTAGCPFRCPYCHNPELVEVSCD
                     GDTEVDGLSSVEEALAFIEHRKPVLSGVVISGGEPTIHAELPALAAAVKRLGLFVKLD
                     TNGCFPERIAAVGADYVAMDLKTTPERYGELWPGAPADAADRIRTAVRAVRGSGAEYE
                     FRITCAPEFVSEIEAEAIARLLEPDDAVYLQRYRPGRTLDPAWAAKVSPYPDERLERI
                     LAIIRRAAPKARLRGF"
     gene            complement(873..>1245)
                     /locus_tag="PLW80_09630"
     CDS             complement(873..>1245)
                     /locus_tag="PLW80_09630"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HPB66814.1"
                     /translation="CEIAGWKGPLHRCSFYGNREVGAKLDAMLASGLEREVDGLLAAG
                     YGRDSPGMKAIGYAEFLDAPIGPDGRRDVRAVAEAIKLHTRRYAKRQLTFMRAIPGVR
                     WFGADDYDGIADAATEWLRSP"
BASE COUNT          190 a          434 c          421 g          200 t
ORIGIN      
        1 tggtcgtcga cgtcgatacc gaggaaggcc tcgagctggc caggcgctac gaggtcatgt
       61 cgacgccgac cgccatcctg atgggctcgg gcggcgagcg gctcggtagg gcccacgccc
      121 gctcccagct cgagcggctg gtcaagcctg agctcgaact cgaggtggcg acggcgtagc
      181 gatgcgcctg ggcatacaga agaccagcct cgtcgatttc cccggacgcg tggccgccgt
      241 gctgttcacg gccggctgcc cattccgttg cccgtattgc cataaccccg aactggtcga
      301 ggtgagctgc gatggggata ccgaggttga cggcctctcg tcggtagagg aggctctggc
      361 cttcatcgag caccgcaagc cggtgctatc gggcgtggtc atctccggcg gcgagccgac
      421 gatccatgcg gagctaccgg ccttggccgc cgccgtcaag cggcttgggt tgttcgtcaa
      481 gctcgacacg aacggttgct ttcccgaacg catcgcggct gtcggcgccg actacgtagc
      541 gatggacctg aagacgaccc cggagcgata cggcgagctg tggcccggag cgccggccga
      601 cgcggccgat aggatcagga ccgccgtgcg cgcggtgcgc ggctcggggg cggagtacga
      661 gttcaggatt acctgcgccc ccgagttcgt ctccgagatc gaggccgagg cgatcgccag
      721 gttgctcgag cccgacgacg ccgtctacct gcaacgctac cgtccggggc gcacgctcga
      781 cccggcctgg gcggccaagg tctctccgta ccccgacgag cggctggaac gcattctggc
      841 catcatccga cgagcagctc ccaaggctag gctcaggggc ttctgagcca ttccgtcgcc
      901 gcgtccgcga tgccgtcgta gtcgtcggcc ccgaaccagc gcacgccggg gatggcgcgc
      961 atgaaggtga gctgccgctt ggcgtagcgc ctcgtatgca gcttgatcgc ctcggccacg
     1021 gcgcgaacgt cacgcctccc gtccggccct atgggagcgt ccaggaactc ggcgtagcct
     1081 atcgccttca tgcccgggga gtcgcgccca tagccggccg ccagcaaccc gtccacctcc
     1141 cgctcgaggc ccgaggcgag catcgcgtcg agctttgcgc ccacttcccg gttgccgtag
     1201 aacgagcagc ggtgcagcgg ccccttccag cccgcgatct cgcag
//
LOCUS       DAOOLN010000785         1244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_148295_length_1244_cov_2.603028, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000785 DAOOLN010000000
VERSION     DAOOLN010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148295_length_1244_cov_2.603028"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PLW80_09635"
     CDS             <1..413
                     /locus_tag="PLW80_09635"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HPB66815.1"
                     /translation="AVVVLAMPIWTAPFGAAFAGPEIATAAGVILGLKGKGVACMLDG
                     FPITAGAVIAWKIDPSVTDHLFAGHLSKVKGHRPVLEALGLEPVVSLDMRLGEGTGAV
                     IGGFIVELGARIAGEMARFSDAAVSKASGEEKDF"
     gene            662..>1244
                     /locus_tag="PLW80_09640"
     CDS             662..>1244
                     /locus_tag="PLW80_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPB66816.1"
                     /translation="MPYDRLVFGNGADELMFAVARAAKRRASGDARGSEDATGPRPEA
                     VIEAPAYASYRDASEAAGLAVSTIPAALPGPADGPCIEVGPEALAEAEAALAAGPEGS
                     VLWLGAPNNPTGLLPAGYPASVVELAGRHPDSLCICDEAFIDFCDGVDPAGAAREAAA
                     MPNLVVLRSMTKFWAVPGLRAGYAVCSRELAAAI"
BASE COUNT          172 a          470 c          416 g          186 t
ORIGIN      
        1 ttgctgtcgt ggtgctcgcc atgccgatct ggacggcgcc cttcggcgcc gccttcgccg
       61 ggcccgagat agccaccgcc gcgggtgtga tactcggcct caagggcaag ggcgtcgcct
      121 gcatgctcga cggcttcccg atcaccgccg gcgcggtcat agcctggaag atagacccga
      181 gcgtcaccga ccacctattc gccggccatc tgtccaaggt caagggtcat cgccccgtgc
      241 tcgaggcgct cgggctcgag ccggtcgtat cgctcgacat gcgcctgggc gagggcacgg
      301 gcgccgtcat cggcggcttc atcgtcgagc tcggagccag gatagccgga gagatggcgc
      361 gcttctcaga cgcggccgtc tccaaagcct ccggcgagga gaaggatttc tgagccacga
      421 cccgcgcgac gccgagcgcg acgagccagg cgccgccctg aagcacggcg gagacctcgt
      481 cgctctcgtc gcggcctccg gcctatcgga ggcctccgtc atcgacgcga gcgccaacct
      541 gaacccgctc ggcccgcccg actggctcga cgccaccttc gccgagggcc gctggcgctc
      601 cgaccgctac cccgacccgc tctaccgtag cctgagagcc gccgcggccg aacggctcgc
      661 cgtgccgtac gatcgcctcg tcttcggtaa cggcgccgac gagctcatgt tcgccgtcgc
      721 ccgcgcggcc aagcggcgag cctccggtga cgcccgcgga tcggaagacg cgacggggcc
      781 gaggccggag gccgtgatcg aggctccagc ctacgcctcc taccgcgacg cgtccgaggc
      841 ggctggcctc gccgtatcca cgataccggc ggcgctcccc gggccggccg atgggccgtg
      901 catcgaggtc ggcccggaag cgctcgccga ggccgaggcc gccctcgcgg ccggcccgga
      961 aggctcagtc ctctggctgg gcgcgccgaa taatcctacc gggctcctac cggccggcta
     1021 tcccgcctcc gtcgtcgagc tggccggccg ccatcccgac agcttgtgta tctgtgacga
     1081 ggccttcatc gatttctgcg acggcgtcga tccagctggc gccgcgcgcg aggccgcggc
     1141 catgccgaac ctcgtggtcc tcagatccat gaccaaattc tgggcagtgc cgggcctgcg
     1201 cgccggttac gccgtctgtt ccagggagct tgcggcggcc atac
//
LOCUS       DAOOLN010000786         1241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_148847_length_1241_cov_2.399663, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000786 DAOOLN010000000
VERSION     DAOOLN010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148847_length_1241_cov_2.399663"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="PLW80_09645"
     CDS             complement(<1..1006)
                     /locus_tag="PLW80_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPB66817.1"
                     /translation="MEAGYQLFKDDETPLYIQAARAVRARILDGELRLGDRLPSVRGL
                     SDELGVNPATVVAAYRILTREGFIVAKAGSGAFVAPDAPVDGAVGEPSAAFPASERPL
                     ADGSSGAVDLAANAPPLDMFPLVDMKRFIVEAIDADGGKAFEYQDTGGYRPLRVAMAE
                     RLAARGRPGSVDPSDVHIVSGAQQGIDLAARILLRPGDVAAVENPGYRGARDAFVAAG
                     ARVEPVPVDSGGLDLDALEALARSRPLRLVHVNPGFQNPTCVVYTAERRSALASMAER
                     YGFYVLEDDQLAELSWDGSMPPAVRDFDEAGRVILVKSTSKCLMPGLRIAFLEAPTSL
                     R"
     gene            complement(1014..>1241)
                     /locus_tag="PLW80_09650"
     CDS             complement(1014..>1241)
                     /locus_tag="PLW80_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPB66818.1"
                     /translation="RGGSTPVRYYQSVFNVGGDVIVAVDGMAVSSLADLYSALEDNKP
                     GDAIRVEYYRGSKRLAATITLSDRAEYLGGK"
BASE COUNT          187 a          418 c          464 g          172 t
ORIGIN      
        1 agcgaaggga ggtcggcgcc tcaaggaagg cgatgcgcag gccgggcatc aggcatttcg
       61 aggtactctt gacgaggatg accctgccgg cctcgtcgaa gtcgcgcacg gccgggggca
      121 tcgacccgtc ccacgatagc tcggcgagct ggtcgtcctc gagcacgtag aagccgtagc
      181 gctcggccat ggaagccagg gccgagcgcc gctcggccgt atagaccacg caggtcgggt
      241 tctggaagcc gggattgacg tggacgaggc gcaggggccg gctcctggcc agggcctcga
      301 gcgcgtccag gtcaaggcct cccgagtcga cgggcactgg ttcgaccctc gctccggcgg
      361 ccacgaaggc gtctcgggcg ccccggtagc cggggttttc cacggcggcc acgtcgccgg
      421 ggcgcagcag gatacgggcc gccaggtcga tcccctgctg ggcgcccgag acgatatgca
      481 cgtcggaggg gtcgaccgag cccgggcgcc cccgggcggc taggcgctcg gccatggcga
      541 cgcgtagcgg gcgatagccg cccgtgtcct ggtactcgaa ggccttgcca ccgtcggcgt
      601 cgatggcctc gacgatgaaa cgcttcatat cgacgagcgg gaacatatcg aggggcggcg
      661 cgttggccgc caggtctacc gcgccgctcg acccgtcggc cagaggccgc tcggacgcag
      721 gaaaggcggc cgacggctcg ccgacggccc cgtcgaccgg cgcgtccggg gcgacaaacg
      781 ctcccgagcc ggccttggcg acgatgaagc cctcgcgggt caggatgcgg taggcggcga
      841 ccacggtggc cggattgacg ccgagctcgt cggagagtcc gcgcaccgag ggcaggcggt
      901 cgccgaggcg gagctcgccg tccaggatgc gggcgcgcac ggcgcgggcg gcctggatat
      961 agagcggcgt ctcgtcgtcc ttgaacagct ggtagccggc ctccatcggg cgattacttg
     1021 ccgcccaggt actcggcccg gtcggacagc gtgatcgtcg cggctagtcg cttggagccg
     1081 cggtagtact ctacccgtat cgcgtcgccg ggcttgttgt cctcgagcgc ggagtagagg
     1141 tccgccagcg agctgaccgc catgccgtcc acggcgacga tcacgtcgcc gccgacgttg
     1201 aacacgctct ggtagtagcg caccggcgtc gagccgccgc g
//
LOCUS       DAOOLN010000787         1241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_148854_length_1241_cov_2.334739, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000787 DAOOLN010000000
VERSION     DAOOLN010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148854_length_1241_cov_2.334739"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..602
                     /locus_tag="PLW80_09655"
     CDS             57..602
                     /locus_tag="PLW80_09655"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HPB66819.1"
                     /translation="MSKYTRLITVLVATLAFAGNAAAQGRGLPKGATVVDGLDKTRYL
                     GTWYEIARYDFAFEKNLSDTTATYALRDDGKIAVTNRGYNYLKGKWTEARAKARFRGS
                     DSVGALEVSFFGPFYAAYNIIALDPEYRYALVVGDSTKYLWILSRTTEIPDDVKAEYL
                     AIAEGLGYDLTAMVWVEHTRH"
     gene            765..>1241
                     /locus_tag="PLW80_09660"
     CDS             765..>1241
                     /locus_tag="PLW80_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66820.1"
                     /translation="MDFYMRLPRDTKEFTLFMFIVSVISVNILAQLITCFELGFSFAV
                     WGRVLRVIPFIWMSVVALVLLTYKPAEWLTYRIVKKGDSFRAVMLASIVSSVLLMSVF
                     LTVIGTWIGSRSFSVEPIRMFFHKWPRNFALSLGVEALVAQPIARQVLLRLHLRKDA"
BASE COUNT          243 a          369 c          349 g          280 t
ORIGIN      
        1 ttttattgtg aaatgcatat atttcatcaa gcggcgcgag gccgcgagga ggaatcatga
       61 gcaagtatac gaggcttata actgttctgg tcgcgactct ggccttcgcg ggcaacgcgg
      121 cggcccaggg ccggggcctg cccaagggcg ccaccgtcgt cgacggcctg gacaagaccc
      181 gctacctcgg cacctggtac gagatagccc gctacgactt cgccttcgag aagaacctga
      241 gcgataccac cgctacctac gccctgcgcg acgacggaaa gatagccgtg accaaccgtg
      301 gctataacta cctcaagggc aagtggaccg aggccagggc caaggcgcgc ttccgcggtt
      361 cggattccgt tggcgccctc gaggtgtcgt tcttcggccc cttctacgcc gcctacaaca
      421 ttatcgcgct cgaccccgag taccgctacg ccctcgtcgt cggcgacagc accaagtacc
      481 tctggatcct gtcgcgcacc accgagatcc ccgacgatgt caaggccgag tacctggcca
      541 tagccgaggg cctcggctat gacctgactg cgatggtctg ggtcgagcac acgaggcatt
      601 gaggctacgc gagtgggttt ttggcttgac tcgataattc cggataggtt ataaaatgaa
      661 ctaaacggat cgcctggcga ttctacattc aatgcgcgta taattgatca gcgcgaaacc
      721 cttggggttt cgcgcttttt ttataccgtg gataaagagg cctcatggat ttttatatgc
      781 gactacctcg cgatacgaag gaattcacgc tgttcatgtt tatcgtctcg gtcatctccg
      841 tcaacatcct cgcgcagttg atcacttgtt tcgagctggg cttcagcttc gccgtatggg
      901 gccgcgtgct ccgcgtcata ccattcatct ggatgagcgt ggtggcgctg gtcctgctga
      961 cctacaagcc ggccgagtgg ctcacgtaca ggatagtcaa gaagggcgac agtttcagag
     1021 ccgtcatgct ggctagtatc gtatcgtccg tactgctcat gtccgtcttc ttgaccgtca
     1081 ttggtacctg gataggtagc cgatcgttca gcgtggaacc gatcaggatg ttcttccata
     1141 agtggccgcg caacttcgct ctatcgctcg gcgtcgaggc tctcgtcgcc cagccgatcg
     1201 ctcgacaggt cttgctgcgc ctgcacctgc gcaaggacgc c
//
LOCUS       DAOOLN010000788         1240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_148998_length_1240_cov_2.983122, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000788 DAOOLN010000000
VERSION     DAOOLN010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148998_length_1240_cov_2.983122"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLW80_09665"
     CDS             <1..196
                     /locus_tag="PLW80_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66821.1"
                     /translation="VVVAKRDPFRIVVGELLESIKRETNQRLASFCRIRTVEHHPDSF
                     QKTPTQKIKRFLYPDRASSR"
     gene            228..>1240
                     /locus_tag="PLW80_09670"
     CDS             228..>1240
                     /locus_tag="PLW80_09670"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPB66822.1"
                     /translation="MPHADDAARPRFPRLAAAQIVASAAAIVFLIPSSCARAPRYAID
                     SSIDDSAVASTLSAYPVPGHWRRVDPRKADIMVSVESSWGVFEDVVPGARTVSAEAYA
                     VAADWLDPRESLRSAETGLVAAAALAEPLSSIATPRKALAYDGLYPGDSGYPVVKSLR
                     VSAVPIGRDGRGVFAAEASAYLDALPEAAPPTGLTWIGAVGDVMPGLGTSAMLLELDG
                     PERVFKRLLPVMRSQDLLIANLETAVTQRGVEWPKTFRFRMPAAALTPLMDSGVDVVG
                     LANNHVYDYTDIGFSDTVAALKAAGLPFIGAGLDWNEALRPFDWSSEYSRSSEYSRSS
                     EYSR"
BASE COUNT          187 a          422 c          394 g          237 t
ORIGIN      
        1 ggtcgtcgtt gcgaaacgcg acccgtttag gatcgtcgta ggcgagctgc tcgagtcgat
       61 caagcgcgag acgaatcagc gcctcgcgtc cttctgccgt attcgcacgg tggagcacca
      121 tcccgactcg ttccagaaga cacctaccca gaagataaag cgcttcttgt acccggatcg
      181 cgcctcctcg cgttgaccgg cagggcgacc gctggtatcc tcgcggtatg ccccatgctg
      241 acgacgcggc caggccgcgc tttccgcgcc tcgccgccgc tcagatcgtc gcgtcagccg
      301 cggcgatagt attcctcatc ccgtcctcct gcgccagggc gcctcgttac gcgatcgact
      361 cgtccattga cgactcagcc gtcgcgtcta cgctgtcggc gtacccggtt ccaggccact
      421 ggaggcgggt cgatccacgc aaggccgaca tcatggtgtc cgtcgagtcg tcctggggcg
      481 tcttcgagga cgtcgtgccg ggcgcccgca cggtgagcgc cgaggcctac gccgtggctg
      541 ccgactggct ggatccgaga gagtctctcc gttcagccga aaccggcctt gttgccgcgg
      601 ccgcgctggc cgaacccctc tcgtcgatag cgacgccccg caaggcgctg gcctacgacg
      661 gtctctaccc cggcgactcc ggctatccgg tggtcaagtc gctgcgagtg agcgcggtgc
      721 ccatcgggcg cgacggccgc ggtgtattcg cagccgaggc atcagcctac ctggatgctc
      781 tgcccgaggc cgcgcccccc accggactga cctggattgg cgccgttggc gatgtgatgc
      841 ctggtttggg tacatcggcc atgctgctcg agctcgatgg gccggagagg gtcttcaagc
      901 gactgctacc ggtgatgcgc tcgcaggacc tgctgatcgc caacctcgag acggccgtga
      961 cccagcgcgg cgtcgaatgg cccaagacct tccgtttcag gatgccggcg gcggcgctta
     1021 cgcccctcat ggacagcggc gtagatgtcg tcggcctggc taataaccat gtctacgact
     1081 acaccgacat tggcttctcc gatactgtgg ctgcgctcaa ggctgccggc ctgccgttca
     1141 tcggcgccgg tctggactgg aatgaggctc tgaggccctt tgactggtcg tctgagtaca
     1201 gccggtcgtc tgagtacagc cggtcgtctg agtacagccg
//
LOCUS       DAOOLN010000789         1237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_149595_length_1237_cov_2.763959, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000789 DAOOLN010000000
VERSION     DAOOLN010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149595_length_1237_cov_2.763959"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /gene="yqeC"
                     /locus_tag="PLW80_09675"
     CDS             complement(<1..454)
                     /gene="yqeC"
                     /locus_tag="PLW80_09675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HPB66823.1"
                     /translation="MNCLSLVAGAPRTTGRPMTVAIVGGGGKTSLLFSLASDAASLGL
                     SALVTTTTRIFDPRDEGRRFDELVLDAGLAAEASRPRPLPSPEAWQRRSVASGRGPVV
                     VAAAGVDGGRLLSVDPARVDDARGWDLVAVEADGARGLPLKAPADHEPV"
     gene            complement(451..>1237)
                     /locus_tag="PLW80_09680"
     CDS             complement(451..>1237)
                     /locus_tag="PLW80_09680"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPB66824.1"
                     /translation="KPFTERFDTILAETGEYPELEAFSPELVFPMRGIDGLYGIVVLG
                     EKVVGNEYSEVETMYLDRLTRFLAVGLQNGLHHQSSITDAKTGLYNHEFFMRRLAEEQ
                     ARCSRSCAQACILMLDVDRFKRFNDSHGHLAGDAALEALAATLKSTMRTGDAVGRFGG
                     EEFCVLALDCDARGAMDAAERVRRSVESLRFSYDGQELSITVSLGIRMLDPGIDPKDL
                     LDDADKALYASKSGGRNRSTLFRPGLLGRAELLRAADPNLCRP"
BASE COUNT          196 a          427 c          424 g          190 t
ORIGIN      
        1 cgaccggctc gtggtcagcc ggagccttga gcggtagtcc cctggcgccg tccgcctcga
       61 ccgcgaccaa gtcccagcct cgagcgtcgt cgacgcgggc cgggtcgacg gacagtagcc
      121 tgccgccgtc gacgccggca gccgccacga ccacggggcc acgccccgac gccacgcttc
      181 gcctctgcca ggcctcggga ctcggcagag gccgcgggcg cgacgcttcc gctgccaagc
      241 ccgcgtcgag aaccagctcg tcgaaacgcc ggccctcgtc gcgcggatcg aagatgcgcg
      301 tcgtcgtcgt gaccagggcc gataggccga gtgaagccgc gtccgaggcc agggagaaca
      361 agaggctcgt cttgccgcct ccgcccacga tggcgaccgt catcgggcgc ccggtcgtac
      421 gcggcgcgcc cgcgacgagg ctcaggcaat tcatggacgg cagagattcg ggtcggccgc
      481 ccgcaggagc tcggcccttc cgagtagccc cggcctgaac agggtggagc ggttgcggcc
      541 tccggacttc gaggcgtaga gcgccttgtc ggcgtcgtcg agcaggtcct tcgggtcgat
      601 cccggggtcg agcatcctga tcccgaggct cacggttatc gagagttcct ggccgtcgta
      661 cgagaagcgc agcgactcca ccgatcgcct gacgcgctcg gccgcgtcca tggccccgcg
      721 agcgtcgcag tccagggcca gcacgcagaa ctcctcgccc ccgaagcgcc ctacggcatc
      781 gccggtacgc atcgtggact tgagcgtcgc ggcgagggct tcgagggcgg cgtcgccggc
      841 caggtggccg tggctgtcgt tgaagcgttt aaagcgatcc acgtcgagca tcaggatgca
      901 ggcctgagcg cagctgcggg aacagcgcgc ctgctcctcg gcgagcctgc gcatgaagaa
      961 ctcgtgattg tagagcccgg tcttggcgtc ggtgatcgaa ctctggtggt ggagcccatt
     1021 ctggaggccg accgccagga agcgcgtcag cctatccagg tacatcgtct ccacctcgga
     1081 gtactcgttg cctacgactt tttccccgag cacgacgatg ccatagaggc catcgatgcc
     1141 gcgcatcggg aacaccagct ccggtgagaa ggcctcgagc tcggggtact cgccggtctc
     1201 cgccaggatg gtgtcgaagc gctcggtaaa gggcttg
//
LOCUS       DAOOLN010000790         1237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_149623_length_1237_cov_2.401015, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000790 DAOOLN010000000
VERSION     DAOOLN010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149623_length_1237_cov_2.401015"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PLW80_09685"
     CDS             <1..337
                     /locus_tag="PLW80_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPB66825.1"
                     /translation="PTASIAIRAGDGSVNVQVGVEVVQRNDSPPTPADAPVVTLLKRA
                     IEETYGVAGRAVGVGGGTVGAYLRSAGFPCVVWSRQDETMHAPNEYTRISNLIGDAKI
                     FARLMLAER"
     gene            689..>1237
                     /locus_tag="PLW80_09690"
     CDS             689..>1237
                     /locus_tag="PLW80_09690"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPB66826.1"
                     /translation="MKSAMLFGAKELRRLTGISAETLRHYVDRDVLRPLRVDGNNYRK
                     FSSQNMIDVLHARIYRGLGLPIQSITRKSGSGPEGQDDVLARHQAILEREALELQMKL
                     ARMRQELEFLATARRRIGCVRVRTADEAPSVYRLTLLDAADGMISDAEVGRRVAEWMR
                     YAPHLHVALRVPEAALLDHGVEE"
BASE COUNT          236 a          400 c          393 g          208 t
ORIGIN      
        1 ccccacggcc tcgatcgcga tccgcgccgg tgacggctcg gtcaacgtgc aggtcggcgt
       61 cgaggtggtg cagcgaaacg atagcccgcc gactccggcc gacgcgcccg tcgtcaccct
      121 gctcaagcgc gccatcgagg agacctacgg cgtggccggc agggccgtcg gcgtgggcgg
      181 cggtaccgtc ggcgcctacc tacgctcggc gggtttcccc tgcgtggtct ggagccgcca
      241 ggacgagacg atgcacgcgc cgaacgagta cacgaggatc agcaacctga tcggcgacgc
      301 caagatcttc gcccgcctga tgctcgccga gaggtaagcc ggtacgccgg tacgccggta
      361 cgcataagac ggcgaagagg atatgagaat aagagcaaag aaagtggaaa tgtaaaagat
      421 taagagactg ataaaggggg cgggaggggc taatacctct tcaccacccc ctcttttccg
      481 cgtaatcctc ttgaatcccc tcgtgtcctc ttctgctctt cgcggatttt cggtatcgtc
      541 gatgacgaag gctaccgtgc acctgcgggc tagctcgcgc cgatggatga gcgcctagac
      601 cgcccatcgc ctgaaccgac agtccgtatc gaccgaccct aggcttctca ctaggcgcgg
      661 tcatggtttt cctgcattat tcgctaccat gaagagcgcc atgctattcg gggctaagga
      721 gctacggcgg ctcacgggca tcagcgccga gacccttcgc cactatgtcg accgcgacgt
      781 cctcaggccg ctccgcgtcg acgggaacaa ttaccgcaaa ttctccagcc agaacatgat
      841 agacgtgctc cacgcccgta tctaccgggg tttgggcctg cccatccaaa gcatcacccg
      901 gaagagcggc tccgggccgg aggggcagga cgacgtgctg gcgcgccatc aggccatcct
      961 cgagcgggag gccctcgagc tgcagatgaa gctcgcccgt atgcggcagg agttggagtt
     1021 cctcgccacg gcgcgtcgca ggatcggatg cgtccgggtc aggacagcgg acgaggcgcc
     1081 gtcggtctat cgcctgaccc tgctcgacgc ggctgacggc atgataagcg acgccgaggt
     1141 cggaaggcgc gtcgccgaat ggatgcgcta cgcccctcac ctccacgtcg cgctacgggt
     1201 gccggaagcc gccttgctcg atcatggcgt cgaagag
//
LOCUS       DAOOLN010000791         1236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_149757_length_1236_cov_3.254022, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000791 DAOOLN010000000
VERSION     DAOOLN010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149757_length_1236_cov_3.254022"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="PLW80_09695"
     CDS             complement(<1..168)
                     /locus_tag="PLW80_09695"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HPB66827.1"
                     /translation="MNAIFDERFKVCTWDVDRADRLTLAAAFNYFQDAAGHHASDLGV
                     GTEYMREHGQVW"
     gene            368..>1236
                     /locus_tag="PLW80_09700"
     CDS             368..>1236
                     /locus_tag="PLW80_09700"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HPB66828.1"
                     /translation="MLVVALALTPALAIIIVSGMEQGAALAEDARRDAARQVEAFAEI
                     QARVTGSTRQMLRTLTALPGIKAMNVGLLSEILRSVHANNPEYLNLTAVDADGVVVAS
                     SLLETGLYLGDRRHFREALDSGEFSSGRYIINMIDETPAIAFAEPIRGESGDVIGAIA
                     AIIKMDSYTGLFENFRLPDESILGMLDADGVRLFFYPPKETNPVGQRIKASVWDDIRA
                     GGDAGLFVETGSDGISRFYAFKKLRLVAGREPYMYVVYAASVDAAYGRSRAVMRRNAA
                     LLIAVAIFAMASSG"
BASE COUNT          220 a          385 c          418 g          213 t
ORIGIN      
        1 ccatacctgg ccgtgctccc tcatgtactc ggtgccgacg ccgaggtcgg aggcgtggtg
       61 gccggccgcg tcctggaagt agttgaaggc cgcggccagg gtcagccggt cggcgcggtc
      121 gacgtcccag gtgcatacct tgaatcgttc atcgaatatc gcgttcatgc ggccactgta
      181 gcaagcgacg cgacggagcg caacggtacc gtcggagttg aagctccgcc cgctccgcac
      241 tagagtggta cgcatggata atccggttca aagcgtcgaa ggccacccgg gggacggcgc
      301 gacccgggtc gcgcgcgcgg gccgtacggg aacggggcgg cgttacctgt ccatacacgc
      361 tacgctgatg ctggtagtcg cgctggcgct gaccccggcg ctcgcaataa tcatcgtcag
      421 cggcatggag cagggagcag cgctggccga ggatgccagg cgtgacgcgg cgaggcaggt
      481 cgaggccttc gcggagatcc aagcgcgcgt caccggttcg acgcgccaga tgctgcgcac
      541 gctgaccgcc ttacccggca tcaaggcgat gaatgtcggg ctcttgtcgg agatactgcg
      601 gtcagttcac gcgaacaacc ccgagtatct caacctaacc gccgtcgatg ccgacggcgt
      661 cgtcgtcgcc tcgtcgctgc tcgagaccgg cctgtacctc ggagaccgga gacacttcag
      721 ggaggcgctc gactcgggcg agttctcttc cggccgctat atcattaata tgatcgacga
      781 gaccccggcc atagcgttcg ccgagccgat acgcggcgaa agcggggacg tcatcggcgc
      841 gatcgcggcc atcattaaga tggacagcta tactggtctt ttcgagaatt tcaggctacc
      901 agacgaatcg atcctgggca tgctcgacgc cgacggggtg cgcctgttct tctacccgcc
      961 caaggagacg aaccccgtcg gtcagcgcat caaggctagc gtctgggacg acatccgggc
     1021 cggcggcgac gcggggctgt tcgtagagac gggatccgac gggatcagcc gcttctacgc
     1081 gttcaagaag ctgcgcttgg tagcggggcg ggagccgtac atgtacgtgg tctacgccgc
     1141 ttcggtcgat gccgcgtacg gcaggagcag ggcggtcatg cggcgcaacg cggccctgct
     1201 gatagctgtc gccatattcg cgatggccag ctccgg
//
LOCUS       DAOOLN010000792         1236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_149793_length_1236_cov_2.513124, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000792 DAOOLN010000000
VERSION     DAOOLN010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149793_length_1236_cov_2.513124"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PLW80_09705"
     CDS             <1..312
                     /locus_tag="PLW80_09705"
                     /inference="COORDINATES: protein motif:HMM:NF036482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66829.1"
                     /translation="PKACAVRDAGIRTNVDADAEGLFVVISAEAPAFYVALEADGLPG
                     RFSDAGFHLAAGETRRLRFLPGAEATSDEAPGAVAPDQPVTADRLSASLRVMDLRSSY
                     E"
     gene            complement(321..1133)
                     /gene="zupT"
                     /locus_tag="PLW80_09710"
     CDS             complement(321..1133)
                     /gene="zupT"
                     /locus_tag="PLW80_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HPB66830.1"
                     /translation="MTMNELSALGLTVFAGLSTGVGSLMALMSRKFNPKFLAASLGFS
                     AGVMIYVSMIEIFVKAKTALTGWYGLKPGNVVTVAAFFGGIAVIAVIDKLIPSYENPH
                     EIHNVAACGDEKAFCVDEQKLRRMGLFSALAIAIHNFPEGLATFMSAISDPALGVSIA
                     VAIAIHNIPEGIAVSAPLYYATKSRRTSFWLSFLSGLSEPVGAIIGYFILKPYFNDGT
                     FGIIFAMVAGIMVYISLDELLPTAEEYGEHHIAIGGLIAGMAVMAGSLLLFI"
BASE COUNT          232 a          398 c          389 g          217 t
ORIGIN      
        1 cccaaggcct gcgccgtccg ggacgcgggc atccggacca atgtcgacgc cgacgccgag
       61 ggcctcttcg tcgtcatttc cgccgaggcc ccggccttct acgtagcgct cgaggccgac
      121 ggcctcccgg gcaggttctc cgacgccggc ttccatctcg cggccggaga gacgcggcgc
      181 ttgcgcttcc tgccgggcgc cgaagccacg agcgacgaag ccccgggcgc cgtcgccccg
      241 gaccaacccg tgacggctga ccggctaagc gcctcgctgc gggtcatgga cctgcgttcg
      301 agctacgagt aggcggaagc ctagatgaac aggagcaggc tacccgccat cacggccatg
      361 cccgcgatga ggccgcctat ggctatgtga tgctcaccgt actcctcggc ggtcggtagc
      421 agttcgtcga gcgatatgta gaccatgatg ccggccacca tcgcgaagat gatgccgaag
      481 gtgccgtcgt tgaagtacgg cttgaggatg aaataaccga taatcgcgcc cacgggctcc
      541 gacaggccgg acaggaacga cagccagaac gacgtgcgtc ggctcttcgt cgcgtagtac
      601 agcggcgccg agacggctat gccttcgggg atgttgtgga tcgctatggc cacggcgatg
      661 gagacgccga gcgccgggtc gctgatcgcg ctcatgaacg tcgccagccc ctcgggaaag
      721 ttgtgtatgg cgatcgctag cgccgagaat agtcccatgc gcctcagctt ctgctcgtcg
      781 acgcagaagg ccttctcgtc gccgcaggcc gccacgttgt ggatctcgtg cgggttctcg
      841 tacgatggta tcagcttatc gatgacggcg atcacggcga taccgccgaa gaacgccgcg
      901 acggtgacga cgttgcctgg cttcaggcca taccacccgg tcagggcggt cttggccttc
      961 acgaaaatct ctatcatcga aacgtagatc atgacgccgg ccgagaagcc gagcgacgcc
     1021 gcgaggaatt tagggttgaa ttttctggac atcaaagcca tcaggctgcc gacgccggtc
     1081 gacaggccgg cgaagacggt caagcccaag gcggacagct cgttcatggt cataggatcg
     1141 tctccttggc tattactata cctaggcgcg agccgaacgg ctatcgccgc gcccccgtat
     1201 ccatccggtc cgagagttgc acgccattat cctata
//
LOCUS       DAOOLN010000793         1235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_149935_length_1235_cov_3.203390, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000793 DAOOLN010000000
VERSION     DAOOLN010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149935_length_1235_cov_3.203390"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..563)
                     /gene="nrdR"
                     /locus_tag="PLW80_09715"
     CDS             complement(93..563)
                     /gene="nrdR"
                     /locus_tag="PLW80_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPB66831.1"
                     /translation="MRCPHCGSMDDRVTDSRTLANGESIRRRRECLSCGFRFTSYERI
                     EEKPLMVVKRDGRREPFDRQKMERGLQRALEKRPVSQMTIENILNEIEDDAAMLGKGG
                     NEVQSSALGEMTLHKLYDLDKVAYIRFASVYRKFETMDEFMQEIERLENAREGE"
     gene            799..1140
                     /locus_tag="PLW80_09720"
     CDS             799..1140
                     /locus_tag="PLW80_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66832.1"
                     /translation="MTFSERMKQLVDQGISASKELARQAGDKAQEWGEKGVLKLEVVQ
                     LRNQAEKLTARLGAEVYEVLVEKGQKSVGKDSPAVRETIDNIEALRAQIEVKEQAFKK
                     AGGKEEDLASD"
BASE COUNT          259 a          367 c          359 g          250 t
ORIGIN      
        1 aaagattcag aatgcgactt gctcaaacga agccgtcctc cgccttcagg aagctgcgcc
       61 actcggggaa gacctgttgc gtctcagcca tctcactctc cctcgcgagc gttctctagc
      121 cgctcgatct cttgcatgaa ctcgtccatg gtctcgaatt tcctatacac gctggcgaaa
      181 cggatgtacg cgactttatc caggtcgtag agcttgtgca gggtcatctc gccgagcgcc
      241 gagctctgga cctcgttgcc gcccttgccg agcatggccg cgtcgtcctc tatctcattg
      301 aggatgttct ctatggtcat ctgggagacc gggcgcttct cgagggcgcg ctggagtcct
      361 cgttccattt tctgccggtc gaagggctcc cgccgtccgt cacgcttgac caccatcagc
      421 ggcttttcct cgatacgctc gtagctcgta aaacggaagc cgcaggacag gcactcgcgg
      481 cgccgcctga tgctctctcc gttggcgagc gtgcgtgagt ccgtgacccg atcatccatc
      541 gatccgcagt gagggcacct cattttttta aacctccaca gaatcgagca gcgggaacaa
      601 cggcggcgca cgacttggta cgattcttgc tattgtacat ataccagtta tagtatccaa
      661 ataccaaacg atagctagat atagtgccta gccgagccct tgtcaagata gcacgatacc
      721 gtggatacgt cattgatttt ttactgtttc cgggcgattt tagttgagag catcatcatg
      781 aacgacaagg agcattgaat gacgttttcc gagcgcatga agcaactcgt cgaccaggga
      841 atcagcgcgt cgaaggagct ggccaggcag gccggcgaca aggcccagga atggggcgag
      901 aaaggcgtgc tcaagctcga ggtggtccag ctgcgcaacc aggccgagaa actgaccgcc
      961 aggctcggcg ccgaggtcta cgaggtactc gtcgagaagg gccagaagag cgtcggcaag
     1021 gattccccgg ccgtccgcga gaccatagac aacatcgagg ccctgcgcgc ccagatcgaa
     1081 gtgaaggaac aggccttcaa gaaggccggc ggcaaggaag aggacctcgc gtcagactga
     1141 gcgcttgcgt atcctggcca cgtagagcgg gccggccccc gaggcgcgat cgggcaggta
     1201 gtggatcccg tactcggtcc gctccgctcc ggcgt
//
LOCUS       DAOOLN010000794         1235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_149946_length_1235_cov_3.017797, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000794 DAOOLN010000000
VERSION     DAOOLN010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149946_length_1235_cov_3.017797"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..946
                     /locus_tag="PLW80_09725"
     CDS             62..946
                     /locus_tag="PLW80_09725"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB66833.1"
                     /translation="MRREPGGLYAAPSTIWLTVFFLAPLAIIVAYSFLTPGVRGGVEW
                     KPTLDAYRAIANPTFAKVTLNTILVAIGATAITLLIALPCGYYMARSERQTLLLMLVM
                     IPFWTNFLIRIYAWIAILGNEGFLNDILLALGLVDESVQFLYNRGAVMLVLVYTYLPY
                     AILPLYSTIDKFDFSLLEAARDLGASKLGAITRVLLPNVKGGILTAFLFTFIPIFGAY
                     AVPLLVGGTESYMLGNLIADELTKSRDWPLASAISLVITLVTTIGVLLLLRANGASTL
                     GGAREAKAEPEPAFGGAR"
     gene            943..>1235
                     /locus_tag="PLW80_09730"
     CDS             943..>1235
                     /locus_tag="PLW80_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HPB66834.1"
                     /translation="MSRPAARKRSSFSRSVFVGTIVFLSLPLLVLVLYSFNESKSGAW
                     TGFSVKWYVKLFTDSPQLWRAFGNSAVVALASAFVATVIGTLGAVAVNWYRFRL"
BASE COUNT          188 a          430 c          379 g          238 t
ORIGIN      
        1 ggaaggacgc cgtttggcta tcgttcagcg ccgaggacgc ctatatcgtc gaggtcaagc
       61 gatgaggcgc gagcccggcg gcctgtacgc ggccccgtcg acgatctggc tgaccgtgtt
      121 cttcctcgcg ccgctcgcca tcatcgtcgc ctacagcttc ctgactccag gcgtccgcgg
      181 cggcgtcgag tggaagccga ccctggacgc gtaccgcgcg atagccaacc cgaccttcgc
      241 caaggtcacg ctgaacacga tactcgtcgc gatcggcgcc acggccataa cgctgctcat
      301 cgccctgccc tgcggctact acatggccag gagcgagcgc cagaccttgc tcctgatgct
      361 cgtgatgata ccgttctgga ccaacttcct gatccgcatc tacgcctgga tagcgatact
      421 cggcaacgag ggcttcttga acgacattct cctcgcgctc ggcctcgtgg acgagagcgt
      481 gcagttcctg tacaaccgcg gcgccgtgat gctggtgctc gtctatacct acctgccgta
      541 tgcgatcctg cccctgtact cgacgataga caagttcgac ttctcgctgc tcgaggcggc
      601 ccgagacctg ggggcgtcca agctaggcgc gatcaccagg gtgctactgc cgaacgtcaa
      661 gggcgggata ctcacggcct tcctattcac cttcatccca atcttcggcg cttacgcggt
      721 gccgctcctc gtcggcggca ccgagtcgta catgctcggt aacctgatag ccgacgagct
      781 gaccaagagc cgggactggc cgctcgcctc ggcgatctcc ctcgtcatca cgctggtgac
      841 gacgataggc gtgctcctgc tcctgcgggc caacggcgcc tccacgctcg gcggcgcccg
      901 ggaggcgaaa gccgagccgg agccggcgtt cgggggcgcc cgatgagccg ccccgcggcg
      961 cggaagcgct cgagcttctc gcggagcgtg ttcgtcggta cgatcgtgtt cctgagcctg
     1021 cccctcctcg tgctggtgct gtactcgttc aacgagtcca agtccggcgc ctggaccggc
     1081 ttctcggtca agtggtacgt caagctgttc accgactcgc cgcagctgtg gcgcgccttc
     1141 ggcaatagcg cggtcgtggc gctggcctcg gccttcgtcg ccaccgtcat aggcacgctc
     1201 ggcgcggtgg cggtcaactg gtaccgtttc cgcct
//
LOCUS       DAOOLN010000795         1235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_149951_length_1235_cov_2.958475, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000795 DAOOLN010000000
VERSION     DAOOLN010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149951_length_1235_cov_2.958475"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..686)
                     /locus_tag="PLW80_09735"
     CDS             complement(93..686)
                     /locus_tag="PLW80_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB66835.1"
                     /translation="MQTSERQKEIIQAAFDLISEQGIQELTIKKIAQVVGVTEPAIYR
                     HFTSKVDILSAVIDEMVAQRNAAFLRCGIGSGTARDMIRSFFAVQASFFESNPSLSIM
                     LFPEDLFRNNADLLGRATSMMDETLARFRELLLAGIDEGSLKPGVDCEAGALMLVGGF
                     RLLVASWRLDAGTGTGAGGLETDTARFMEGVLSLISI"
     gene            844..>1235
                     /locus_tag="PLW80_09740"
     CDS             844..>1235
                     /locus_tag="PLW80_09740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66836.1"
                     /translation="MPMQRTQRTEAPDRLVRTPYLWSLYLLLGLFSFMLSMIGPMVPY
                     LRDEFSLSYGLAGLHQSAFALGMVIMGFLGSSVIRRLGTALSLWGGMADMLAGLLVMI
                     LAHGPAMTLTGVFVMSLGGTVALAAIQTS"
BASE COUNT          247 a          389 c          358 g          241 t
ORIGIN      
        1 tctgcattcg caaccccttt ggtgtggcaa catgagttag ttatcgttca caaaccttag
       61 cagataaaaa aatgatcgcg gcaaggacga tgttatatgc tgatcaggga gagcacccct
      121 tccatgaagc gagctgtatc cgtctcaaga ccgccggccc cggttcccgt tccggcgtcc
      181 agccgccagg acgccaccag caggcggaag ccgccgacga gcataagcgc gccggcctcg
      241 cagtcgacgc cgggtttaag cgagccttcg tcgatccccg ccaacagcag ttcgcggaac
      301 cgggcgaggg tctcgtccat catcgatgtc gccctgccca gcaggtcggc gttgttgcgg
      361 aacagatcct cggggaacag catgatggac aacgatggat tgctctcgaa aaagctggcc
      421 tgtacggcga agaacgagcg gatcatatcg cgagccgtac ctgaaccgat cccgcaccgc
      481 aggaaggcgg cgtttcgctg cgcgaccatt tcgtctatga ccgcggacag gatatcgacc
      541 ttcgacgtga aatggcggta gatggcgggt tcggtgactc cgacgacctg ggcgatcttt
      601 tttatggtga gttcctggat gccctgttca gagatcaggt cgaacgccgc ctgaatgatc
      661 tctttctgcc gttcgctagt ctgcaccgat tctccccacc aagttagtga tcactaacct
      721 acagtacaac gagaaggaac gtcaagacgt ctaccaaaaa aacgaccccg gagggcacgc
      781 aagctcgaac ctggatcgga gcccctcgat gacagcgcgg tcgaaatagg ctatacctta
      841 tgcatgccca tgcaacgaac acagcgaacg gaagcgcccg accgactcgt ccgcacgccg
      901 tatctatggt cgctctacct gctcctcggc ctgttctcgt tcatgctgtc gatgataggg
      961 ccgatggtgc cgtacctgcg ggacgagttc tcgctcagct acggcctggc cggcctccat
     1021 cagagcgcct tcgcgctcgg catggtcatc atgggcttcc tcggcagttc ggtgatccgg
     1081 cgcctcggca cggcgctgag cctctggggc ggcatggccg acatgctggc cggcctcctc
     1141 gtgatgatcc tggcgcacgg cccggctatg accctgacag gcgtcttcgt gatgagcctc
     1201 ggcggcaccg tggcgctcgc cgccatccag acctc
//
LOCUS       DAOOLN010000796         1235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_149973_length_1235_cov_2.627119, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000796 DAOOLN010000000
VERSION     DAOOLN010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149973_length_1235_cov_2.627119"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..>1235)
                     /locus_tag="PLW80_09745"
     CDS             complement(266..>1235)
                     /locus_tag="PLW80_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPB66837.1"
                     /translation="EYGASGVCEVLDPYIKATKFDLGDFGSGLKVASSYGGKQVSLPF
                     LISTQIMFYNKDMATAEKIKLPETWAEMDSFMQKASKVSGGVAQRYATVIPGWDQWYF
                     EPFFLNNGIAIVNKDGETTDLGAPAAIAVASKLKQWCDSKYAYWAYGKDASSVMRQNF
                     IEGKTFSVIHTTSLYNMYATNCKFEVGMHYLPGAKTRDSEIGGCVLLIPRKNSQEVKN
                     AGWKLLSFLTGKEVNMLWARETGYMPTRNSVTRTSEGKAFLAEKPAFKAVFDNLDHIN
                     PRIQHTAYSALSKIWMQELAKVIIEGGDVAQAMKKASILIDEALQD"
BASE COUNT          203 a          384 c          393 g          255 t
ORIGIN      
        1 tacttgtact cgtcccggat caggttccag ttcgtgaagc ctatcctgaa caggtcgacg
       61 agcgggtagt acacgaacac ggcgaagaag gccagcgccg gcgccacgca taggaaatcc
      121 ttgaaccgct ctctcctgac ccgcggcgac gtccgcctcc tggtcgcatc caccgccgta
      181 tctcgttccc gcacgtttcc cctccggata aaaaggcggg ccggccccgg ggccggctcc
      241 gctcgccgcg ctagggcacg tcgccctagt cctgtagcgc ctcgtcgatc agtatcgagg
      301 ccttcttcat cgcctgcgcg acgtctccgc cctcgatgat gaccttggcc agctcctgca
      361 tccagatctt ggacagcgcc gagtaggcgg tgtgctggat gcgcgggttg atatggtcga
      421 ggttgtcgaa caccgccttg aaggccggct tctcggcgag gaaggccttg ccctcggagg
      481 tcctggtgac cgagttcctc gtgggcatgt agccggtctc cctggcccag agcatgttga
      541 cttccttgcc ggtcaggaag gacagcagct tccacccggc gttcttgacc tcctgggagt
      601 tcttcctcgg gatcaggagc acgcagccgc cgatctcgga gtccctggtc ttggcgccgg
      661 gcaggtagtg catgccgacc tcgaacttgc agttggtcgc gtacatgtta tagagcgagg
      721 tggtgtggat caccgagaag gtcttgccct cgatgaagtt ctggcgcatc acgctcgacg
      781 cgtccttgcc gtaggcccag tacgcgtact tgctgtcgca ccactgcttg agcttggacg
      841 cgacggctat ggcggcgggg gcgccgaggt cggtggtctc gccgtcctta ttgacgatgg
      901 cgatgccgtt gttcaggaag aagggctcga agtaccactg gtcccagccg ggaatcacgg
      961 tggcgtagcg ctgggcgacg ccgcccgaaa ccttggaagc cttctgcatg aacgagtcca
     1021 tctcggccca ggtctccggc agcttaatct tctcggccgt ggccatgtcc ttgttgtaga
     1081 acatgatctg ggtggagatg aggaagggca gcgatacctg cttgccgccg tagctcgagg
     1141 cgaccttgag gcccgagccg aagtcgccca agtcgaactt ggtggccttg atgtaggggt
     1201 cgagcacctc gcagacgcct gaggcgccgt actcg
//
LOCUS       DAOOLN010000797         1234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_150152_length_1234_cov_3.023749, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000797 DAOOLN010000000
VERSION     DAOOLN010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150152_length_1234_cov_3.023749"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="PLW80_09750"
     CDS             complement(<1..1000)
                     /locus_tag="PLW80_09750"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HPB66838.1"
                     /translation="MPPDFYARPQTNTALLIPSSAIEGGTVSLALRCYYSGTRARIPG
                     FALADSSRADSLQYLASFFVSRINIILAVLCAFMGVYFAAGFVSKRDDTASLFYALSL
                     FFVAFYFFDMGTERLMLGGLLQRAVARSCLTASLCFSLLFFIRFFDTRGFKAAKVFAA
                     LDITAFTAAFIVFSNDDGVVDLLFNLSLAPVFAIIVIALAILVKATRKGQRDALPILI
                     GILVGVGFAVHDIVFQVSGRQPFAWLQGFTFFFLNLSIFIATSLRSSTLRRELDAYTA
                     ELSSQRDKLSSLLREVERAADETSSVAQTLEAEVAEVADEARRSAASANDIGVSASA"
     gene            1058..1198
                     /locus_tag="PLW80_09755"
     CDS             1058..1198
                     /locus_tag="PLW80_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66839.1"
                     /translation="MPLSNQIGPAAMDGGTEASALIQKYPSPPRRSTTAGARTTSHDE
                     SS"
BASE COUNT          265 a          373 c          421 g          175 t
ORIGIN      
        1 gagctgacgc cgagacgcct atgtcgttag cggaggctgc cgagcgccta gcctcgtccg
       61 ccacctccgc cacctccgcc tcgagcgtct gggctacgct cgaggtctcg tcggcagccc
      121 gctccacctc gcgcaggagc gagctcagct tgtcccgctg ggacgaaagt tcggcggtgt
      181 aggcgtcgag ctcccggcgt agcgtcgagg atcgcagcga ggtggctatg aaaatggaaa
      241 ggttgaggaa gaagaaggtg aagccctgga gccaggcgaa gggctgcctg ccggacacct
      301 ggaacacgat gtcgtggacg gcgaagccga ctccgacgag tatacctatc aggatgggca
      361 acgcgtcgcg ctgacccttc ctggtcgcct tgacgagtat ggccagggcg atgactatga
      421 tagcgaatac cggcgccagg ctcaggttga acaataggtc gaccacgcca tcgtcgttcg
      481 agaagacgat gaaagcggcg gtgaacgccg tgatgtcgag ggcggcgaag accttcgcgg
      541 ccttgaagcc ccgggtatcg aagaaccgta tgaagaacag gagcgagaag cacagcgacg
      601 ccgtaaggca cgacctagcg acggcacgct gcaaaaggcc acctagcatg aggcgctcgg
      661 tgcccatatc gaaaaagtag aaagccacga agaataggga caatgcgtag aagagactgg
      721 ccgtatcgtc gcgtttggag acgaaaccgg ccgcgaagta gacgcccatg aaggcgcata
      781 gcacggcgag tattatgttg atcctggaga cgaagaagga cgcgaggtac tggagcgagt
      841 cggctctcga ggagtccgcc agcgcgaagc ccgggatcct ggctcgggta cccgagtagt
      901 agcagcgcag ggccaaggac accgtgccgc cttcgatggc cgacgacggg atgagcagcg
      961 ccgtgttcgt ctgcggcctg gcgtaaaagt caggcggcaa cccgcctcgg gagccgatgt
     1021 agacgccccg gcgtacaggt cgaaggcgca gtccatcttg ccgctctcga accagatcgg
     1081 cccggccgcc atggacggcg gcaccgaggc ctcggccctg atccagaagt acccgtcgcc
     1141 gccgcgcagg tcgacgacgg ccggagcgcg tacgacctcc cacgacgagt cgtcgtagcc
     1201 cggcgacgcc caggccggat cgtcgccgcg gccg
//
LOCUS       DAOOLN010000798         1233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_150379_length_1233_cov_2.823430, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000798 DAOOLN010000000
VERSION     DAOOLN010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150379_length_1233_cov_2.823430"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="PLW80_09760"
     CDS             <1..1020
                     /locus_tag="PLW80_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66840.1"
                     /translation="APASLDAELDALVAKARDAIDANHLSEGIKFYISALAKATNGGR
                     RSRADEITGTLNAIGARLTIEPHESWLEPDGSQRSGSTRQAARGEGLQPAVYLYESYG
                     YAKSAVPDAFIRFEFVSNGGKLVASVATDAKGMANTSIGAIDSAGSDAVVRAFPIFTS
                     EGYSYAFRSVFRDFSYVAPPNLAIVAALERTPAGYSSNPRVLDAVGQSLKPLGVEVAP
                     YNGVLAPAGFEAAFAGDVRALASLAGTVKAGYFALVYVEVAAPTRMEYGGKVYNIYIA
                     NAKATLRIVRVDGTVVFAEAKDGVRGQGGTEQASIDDCLVKVRDELSAIISGKSEAIR
                     KALAE"
     gene            complement(998..>1233)
                     /locus_tag="PLW80_09765"
     CDS             complement(998..>1233)
                     /locus_tag="PLW80_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66841.1"
                     /translation="ARSLLARKWDGRPLRLVGLALACLERGAARQAGLFDDGDDRVAR
                     VERAGIEAARRGLGKLTRARLLHGPGPTRPKPS"
BASE COUNT          205 a          444 c          403 g          181 t
ORIGIN      
        1 gcccccgcct cgctcgacgc cgagcttgac gccctcgtcg ccaaggcccg cgacgccata
       61 gacgcgaacc acctgtccga gggcatcaag ttctatatat ccgccctcgc caaggcgacg
      121 aatggcggca ggcgatcgag agccgacgag atcaccggga cgctcaacgc gatcggagcc
      181 aggctgacta tagaaccgca cgagtcctgg ctcgagccgg acgggtcgca gaggtccggt
      241 agcaccaggc aggcggctcg cggcgagggt ttgcagcctg cggtctacct ctacgagagc
      301 tatggctacg cgaagagcgc cgtgccggac gccttcatcc gcttcgagtt cgtgtccaac
      361 ggaggcaagc tcgtcgcgtc ggtcgcgacc gacgccaagg gcatggccaa cacgagcatc
      421 ggcgcgatag actccgccgg ttccgacgcc gtcgtcagag ccttcccgat ctttacgtcc
      481 gaaggctaca gctacgcgtt caggtcggtg ttccgcgact tcagctacgt cgcgccgccg
      541 aacctcgcca tcgtggccgc gctcgaacgg accccggccg gctatagctc caacccgcgc
      601 gtgctcgacg cggtcggcca gtcgctcaag ccgctcggcg tcgaggtggc tccgtacaac
      661 ggcgtgctcg cgcccgctgg cttcgaggcg gccttcgccg gagacgtacg ggccctggcc
      721 tcgttggccg gcacggtcaa ggccggctat ttcgcgctcg tctacgtcga agtggccgcg
      781 cctaccagga tggaatacgg cggcaaggtc tacaacatct acatagccaa cgccaaggcc
      841 accctgcgca tcgtccgggt cgacggcacc gtcgtgttcg ccgaggcaaa ggacggcgtc
      901 cgcggccaag gcggcaccga gcaggcctcg atcgacgatt gcctggtcaa ggtcagggac
      961 gagctgtcgg ccatcatctc gggcaagtcc gaggctatca ggaaggcttt ggccgagtag
     1021 gtcccggccc gtgtaggagc cgcgcccggg taagcttgcc gagcccgcgg cgcgccgcct
     1081 cgatgccggc ccgctcgacg cgggcaaccc tgtcgtcgcc atcgtcgaac agccccgcct
     1141 gcctcgccgc tccgcgctcc aggcaggcga gcgccaaccc gacgaggcgt agcggccgcc
     1201 cgtcccactt gcgcgccagc agggagcgcg ccg
//
LOCUS       DAOOLN010000799         1233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_150422_length_1233_cov_2.405772, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000799 DAOOLN010000000
VERSION     DAOOLN010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150422_length_1233_cov_2.405772"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1233)
                     /locus_tag="PLW80_09770"
     CDS             complement(<1..>1233)
                     /locus_tag="PLW80_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HPB66842.1"
                     /translation="VGARAEREAAREAERAARAAAKAAPEPPADTADDDFETRTFDLS
                     YPDLPPVPFLASMTAHEPAPEAAGTVMADTSLGAKPAPEGYGGDSGDDGAAGDDEPEL
                     LVDLDDAHPEADEPIPLMFGRTEPRDDVRADLVASDPARDADFWADAGLVDAEPAADE
                     PAAADRRAHDAPDERRPSLLPRVEVFQDGEHAEALRTNGEARRDFDPAAVTPAATLVK
                     AARPVPEESSTPPAKPAATPPEGEPVAAKPRLPGRKPVVAYSMPTEGILTDYPDGQYW
                     IIDDKTRKAAEVLRETLQEFGIEAEVTGIRKGPVITMFEILPAPGVRLSKIANLCDNI
                     ALRLAASSVRIVAPIPGKHAVGIEVPNERRAIVSFKEIVESETFKNAKMEVPVVLGKD
                     ITGDVQVVELTHTPHLLI"
BASE COUNT          145 a          429 c          451 g          208 t
ORIGIN      
        1 cgatcaggag atgcggggta tgggtcagct cgacgacctg aacgtcgccg gtgatatcct
       61 tgcccagcac gaccggcacc tccatcttgg cgttcttgaa ggtctcgctc tcgacgatct
      121 ccttgaacga cacgatggcc cgccgctcgt tcggtacctc gatgccgacg gcatgtttgc
      181 ccggtatcgg ggcgacgatg cgcacgctcg aggcggccag cctgagcgcg atattgtcgc
      241 agaggttggc tatcttggag agccgcacgc ccggcgcggg cagtatctcg aacatggtga
      301 tgaccgggcc cttgcgtatg ccggtgacct cggcctcgat gccgaactcc tggagcgtct
      361 cgcggaggac ctcggcggct ttcctggtct tatcgtcgat gatccagtac tggccgtcgg
      421 gatagtcagt caggatgccc tctgtcggca tcgagtacgc gacgaccggc ttgcggccgg
      481 gcaggcgggg cttcgcggcc acgggctcgc cttcgggcgg cgtggcggcc ggcttggccg
      541 gcggcgtcga cgactcctcg ggcacgggcc tggccgcctt gaccagcgtc gcggccggcg
      601 tcacggcggc cgggtcgaag tcccgcctgg cctcgccgtt cgttcgcagc gcctcggcgt
      661 gctcgccgtc ctggaacacc tcgaccctcg gcagcaggga cggccgcctc tcgtccggag
      721 cgtcgtgagc gcgtcggtca gcggccgcag gctcgtcggc cgcgggctcg gcgtcgacga
      781 ggccggcgtc ggcccagaag tcggcgtcgc gggcgggatc gctggcgacc aggtcggccc
      841 ggacgtcgtc cctcggctcg gtccggccga acatcagcgg tatcggctcg tcggcctcgg
      901 gatgggcgtc gtcgaggtcg acgagcaatt cgggctcatc gtcgcctgcg gcgccgtcgt
      961 cgcccgagtc gccgccatag ccctcgggcg cgggcttcgc gccgagcgat gtatcggcca
     1021 tcacggtgcc cgcggcctcg ggagccggct cgtgggcggt catcgacgcc aggaagggca
     1081 ccggaggcag gtcgggatag ctcaggtcga aggtgcgcgt ctcgaagtcg tcgtcggccg
     1141 tatcggccgg cggctccggc gcggccttcg cggccgcgcg ggcggcccgc tccgcttcac
     1201 gggccgcttc ccgctcggct cgcgccccta cgc
//
LOCUS       DAOOLN010000800         1231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_150773_length_1231_cov_2.831633, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000800 DAOOLN010000000
VERSION     DAOOLN010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150773_length_1231_cov_2.831633"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..>1231
                     /locus_tag="PLW80_09775"
     CDS             166..>1231
                     /locus_tag="PLW80_09775"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HPB66843.1"
                     /translation="MKKKYSLDRVLPGSRFTADAFSDGNNLFVPAGVKVRAKDLEGLK
                     RWGITAVFSEGQLESPDEKPVGQDSSSGAGTGHDDGRAPASKGLAASFSDHALYASYT
                     DMIDRLKALMDKIAASERVEPKHIDSLSQDLVQLVREKEAVVVSAILSSEFSGYDMAR
                     AGINCAILSVVVGQTMKLPPHRLVHLASGALLHDVGMLRVPQEIIKKKGALSPEEAQK
                     MRAHPLLTYRIVIKELQYPDDVGLVGLQHHERWDGEGYPRRTSGQGIDLLARIVSVAD
                     AFEAMVSQKPYRNSMIGYAAMKNLLSDNSRRFDPDVLKAFVRSMGIYPIGSIVVLNNA
                     AIARVVDTHAGAGLYALDSDW"
BASE COUNT          227 a          382 c          400 g          222 t
ORIGIN      
        1 agtggccgag cgcatcgagg cgatagcgac ccggacgggc gtgccgctgg tccgcgatga
       61 cgtcgtggct gcggccctcg aacccgtcga tatcggcgag ttcgtgccac cggagtattg
      121 ggaactcgta gcgcgcgtgt tcgtctttat aaggaaagtg cgatcatgaa gaagaagtat
      181 tcgctcgaca gggtcttgcc aggctctcga ttcaccgccg acgcgttctc ggacggtaat
      241 aacctattcg tgcccgccgg cgtcaaggtc agggccaagg acctcgaggg gctcaagcgc
      301 tggggtatta ccgcggtatt cagcgagggc cagctcgagt ctccggacga gaagcccgtg
      361 ggccaggact cctcgtccgg cgccgggacc ggccacgacg acgggcgcgc ccccgcgtcg
      421 aaggggctcg cggcgtcgtt ctcggatcac gcgctctacg cgtcctatac ggatatgatc
      481 gacaggctca aggccctgat ggataagatc gccgcgagcg aacgcgtgga acccaagcat
      541 atagactccc tgtcgcagga tctcgttcag ctggtacgcg agaaggaggc cgtggtcgtt
      601 tcggctatcc tgtcctcgga gttctcgggt tacgatatgg ccagggcggg catcaattgc
      661 gccatcctgt cggtcgtggt cggccagacc atgaagctgc cgccccatag gctcgtgcac
      721 ctggcgtccg gcgccctcct gcacgacgtc ggcatgctgc gcgtgcctca ggagatcata
      781 aagaagaaag gcgcgctcag ccccgaggag gcgcagaaga tgcgcgccca tccgctcctg
      841 acctaccgca tcgtcatcaa ggagctgcag tatccggacg acgtcggcct ggtcggcctt
      901 cagcatcacg agcgatggga cggagaggga tacccgcgcc ggacgagtgg ccagggcatc
      961 gatctgctcg ctcgcatcgt gtcggtcgcc gacgctttcg aggccatggt cagccaaaag
     1021 ccgtaccgga attcgatgat aggctacgcc gcgatgaaga atctactgtc ggacaactcg
     1081 cggcgtttcg accccgacgt gctcaaggcg ttcgtccgta gcatgggcat ctatcctatc
     1141 ggctccatag tcgtgctgaa caacgccgcc atcgcgcggg tcgtcgatac tcacgccggt
     1201 gcgggcctgt acgcgctcga cagcgactgg g
//
LOCUS       DAOOLN010000801         1231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_150861_length_1231_cov_1.937925, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000801 DAOOLN010000000
VERSION     DAOOLN010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150861_length_1231_cov_1.937925"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PLW80_09780"
     CDS             <1..205
                     /locus_tag="PLW80_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acyl-D-aspartate deacylase"
                     /protein_id="HPB66844.1"
                     /translation="LCGHSSLRALRVEDLGRPASPDELAAMMADLEAALDAGCAGLSA
                     GLAYPAAIASSTDEVTALARAAF"
     gene            296..1015
                     /locus_tag="PLW80_09785"
     CDS             296..1015
                     /locus_tag="PLW80_09785"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB66845.1"
                     /translation="MPGSPEPGARLVLSHQKCAGPVQRGRASELLERIERAARDIPLA
                     FDAYPYEAGSTMLEAESVRASRRVLVTWSEPHPELAGLELSEAAERLGLDEQATVEAL
                     KPGGGAYFHMDEADVDAILAHPLCMVGSDGIPADAVPHPRLYGAFPRYFGRMVREKRL
                     LGLEEALRKVTSLPAAVFSLGSKGVLCEGADADIVVFEPELYRDRADWREPRRYCEGV
                     RAVLRRGELRSAARFEELGNQ"
     gene            complement(1044..>1231)
                     /locus_tag="PLW80_09790"
     CDS             complement(1044..>1231)
                     /locus_tag="PLW80_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66846.1"
                     /translation="NFYFVMTDRFDNGTPGNDACIATGHAPEGGELPVDCAGTDKLVA
                     GVAAHYIYLGVRLLGWA"
BASE COUNT          168 a          430 c          435 g          198 t
ORIGIN      
        1 tctctgcggc cactcgtcgc tacgggcgct acgcgtggag gacctcgggc ggcccgcctc
       61 tcccgacgag ctcgcggcga tgatggccga cctcgaggcg gcgctcgacg ccggctgcgc
      121 cgggctgtcg gcggggctcg cctatccggc ggccatagca tcgtcgaccg acgaggtaac
      181 ggccctcgcc cgcgcggcgt tctgacgggg cggtgtcttc gcgatccacc tgcggaacga
      241 gtacgacggc gtctgggacg cgctcgacga ggccttcgcg atcgctcggg cggccttgcc
      301 cggttcgccc gagccgggcg ctcgcctcgt cctttcgcat cagaagtgcg ccgggccggt
      361 ccagcgcggc cgggcgtccg agctgctcga gcgcatcgag cgcgccgctc gcgatatacc
      421 gctcgccttc gacgcgtatc cttacgaggc cggttcgacg atgctcgagg ccgagtcggt
      481 acgagcctct cgccgcgtgc tcgtcacctg gtcggagccg catccggagc tggccggcct
      541 cgagctgagc gaggccgccg agcggctcgg gctggacgag caagccaccg tcgaggcgct
      601 gaagccgggc ggcggcgcgt atttccacat ggacgaggcc gacgtcgacg cgatcctggc
      661 ccatcccctg tgcatggtcg gttccgacgg catcccggcc gacgccgtac cgcacccgcg
      721 cctgtacggc gccttcccgc gttacttcgg ccgcatggta cgagagaagc ggctcctcgg
      781 gctcgaggag gccctgcgca aggtcacctc cttgcccgcg gccgttttct cgctcggctc
      841 gaagggcgtc ctctgcgagg gggccgacgc cgacatcgtc gtgttcgaac cggagctgta
      901 ccgcgaccgc gccgattggc gcgagccgcg gcggtactgc gaaggggtcc gcgcggtgct
      961 acgccgcggc gagctaaggt ccgctgcaag gttcgaggaa ctaggtaacc aataagcttc
     1021 gtaacactac cggggaatcc ggcttaggcc cagccgagca ggcggactcc caggtagata
     1081 tagtgggccg cgacgccggc gacgagcttg tcggtgcccg cgcagtcgac ggggagctcc
     1141 cctccctcag gtgcgtgtcc ggtcgcgatg caggcatcgt tgcccggcgt cccgttgtcg
     1201 aaacggtcgg tcatcacgaa gtagaagttg t
//
LOCUS       DAOOLN010000802         1230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_150944_length_1230_cov_3.401702, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000802 DAOOLN010000000
VERSION     DAOOLN010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150944_length_1230_cov_3.401702"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PLW80_09795"
     CDS             complement(<1..617)
                     /locus_tag="PLW80_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPB66847.1"
                     /translation="MAARAPRPLPEYLAGLNPEQLEAVLHEDGNLLILAGAGSGKTRV
                     ITTKIAYLIAERGLAPESILAVTFTNKAAAEMRERACAIEPACARANLRTFHSFGAWF
                     LRRNAAAAGLDRDFTIYDDDDSTTLLRAAFPELSRPEAGKLASAIARAKDYGLTPDAP
                     DLERGFGGKDFRRRYAEYEGRLRQTGNVDFGDLISLPVRVLEREPA"
     gene            complement(607..>1230)
                     /locus_tag="PLW80_09800"
     CDS             complement(607..>1230)
                     /locus_tag="PLW80_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB66848.1"
                     /translation="HPDRFPSGAIDACSRDARILPYFDLPFQHASRRVLRAMNRSGDA
                     ERYLGLLSDIRSALPDAVIRSTFLVGFPGESDDEFAELRAFQDAARVDWLGAFSYSRE
                     EDTPAYSMPGRVSKRVAAARKKAVEDAQRPITEASLGRFVDTAQTALVEERIEGDDLA
                     IARGYMNAPDVDGSIVVVGGDLAPGDVVPVTITAVRGVDLEAVPRGR"
BASE COUNT          178 a          389 c          478 g          185 t
ORIGIN      
        1 gccggctcgc gctcgagcac cctgaccggc agggagatga ggtcgccgaa gtcgacgttg
       61 ccggtctgcc gtagccggcc ctcgtactcg gcatagcggc ggcggaagtc cttgccgccg
      121 aagccgcgct cgaggtctgg cgcgtcgggc gtcaggccgt agtccttggc tcgggcgatg
      181 gccgaggcca gcttgcccgc ctccggcctg gatagctcgg ggaaggcggc gcggagcagg
      241 gtggtcgagt cgtcgtcgtc gtagatcgtg aagtcgcggt ccagcccggc ggccgcggcg
      301 ttgcgcctta agaaccaggc gccgaacgag tggaaggtac gcaggttggc cctggcgcag
      361 gccggctcga tggcgcaggc tcgctcgcgc atctcggctg ccgccttatt ggtgaaggtg
      421 accgccagga tcgactcggg cgcgaggccg cgctcggcta tcaggtaggc tatcttcgtg
      481 gtgatcacgc gggtcttacc cgagccggcg ccggcgagga tcagcaggtt accgtcctca
      541 tgcagcacgg cctcgagctg ttcgggattg aggccggcga gatactcggg aaggggcctc
      601 ggggccctag cggccacggg gcaccgcctc gaggtcgacg ccccgcaccg ccgtgatggt
      661 caccggtacg acgtcgccgg gggccaggtc gccgcccacg acgactatgg agccgtcgac
      721 gtctggcgcg ttcatgtagc cgcgggctat ggccaggtcg tcgccctcga tgcgctcctc
      781 gacgagcgcc gtctgggcgg tatcgacgaa gcgcccgagc gacgcctcgg tgatggggcg
      841 ctgggcgtcc tcgacggcct tcttgcgggc ggcggcgacg cgcttggaca cccggcccgg
      901 catcgagtag gccggagtat cctcctcgcg gctgtaggag aaggcgccga gccagtcgac
      961 gcgggccgcg tcctggaagg cgcgcagttc ggcgaactcg tcgtcgctct cgcccggaaa
     1021 gccgaccagg aaggtcgagc ggatcaccgc gtcgggtagg gcggagcgta tgtcggagag
     1081 gaggccgaga tagcgctcgg cgtcgcccga gcggttcatc gcgcggagca cgcgacggga
     1141 agcgtgttgg aacggtaggt cgaagtaggg caggatgcgc gcgtcgcgcg agcaggcgtc
     1201 tatcgcgccc gaggggaagc ggtcaggatg
//
LOCUS       DAOOLN010000803         1230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_150991_length_1230_cov_2.576170, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000803 DAOOLN010000000
VERSION     DAOOLN010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150991_length_1230_cov_2.576170"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1030)
                     /gene="ispH"
                     /locus_tag="PLW80_09805"
     CDS             complement(134..1030)
                     /gene="ispH"
                     /locus_tag="PLW80_09805"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPB66849.1"
                     /translation="MTVTRARTLGFCPGVRRAVETAERAAAAASASGSSGFVLGQIVH
                     NPRVSARLSGLGMETIADPDDAPGRLAGATVVIRSHGAAPEVFERLRAAGAEVVDATC
                     PRVAANQKAAASYASRGYLVVIAGDAGHGETLGVAGHAPGAVIVSSAAEAAAVATSRP
                     LALIAQTTISETEYEAVREALRIPGRELVDVGGVCAASRERRSAIEELAGEVDAIVVV
                     GGKNSANTRRLAELAATLGVPTWHVENAAEIPDELGRYERVGLSAGASTPDEDIDEAE
                     ARLFELGQAASDDAASDAASRA"
     gene            complement(1048..>1230)
                     /locus_tag="PLW80_09810"
     CDS             complement(1048..>1230)
                     /locus_tag="PLW80_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPB66850.1"
                     /translation="YNVMAVGMETAALYTIAAKFRKRALTLLTVSDSIVTGEATSAEE
                     RQTTFKTMMEIALELA"
BASE COUNT          149 a          461 c          426 g          194 t
ORIGIN      
        1 tcttcaagcc gaccatccgc gtctacgagg tagccaacac gggcttctgg tacggcctgg
       61 gcttctggct cgccatcggg ggcggcaccg gaggcggatg cgccgcggcg ggccggcgta
      121 agcgctaccg ggcctaagcc cggcttgccg catcgctcgc ggcatcatcg ctcgcggctt
      181 ggccgagttc gaacagccta gcctcggcct cgtctatgtc ctcgtccggc gtcgaggcgc
      241 cggccgacag gccgacccgt tcgtagcggc cgagctcgtc gggaatctcg gccgcattct
      301 cgacgtgcca ggtcggtacg ccgagcgtcg cggccagctc ggccaggcgc ctcgtgttgg
      361 cggagttctt ccctccgacg accacgatcg cgtcgacctc gccggccagc tcctcgatgg
      421 cggagcggcg ctcgcggctg gccgcgcaga cgcccccgac gtcgacgagt tcgcgcccgg
      481 gaatgcggag cgcctcccgt acggcttcgt attcggtctc cgagatcgtc gtctgcgcta
      541 tcagggccag gggccggctc gtcgccaccg cagccgcctc cgcggcgctc gatacgatga
      601 cggcgcccgg cgcgtggccg gccacgccga gggtctcgcc gtggcccgcg tcgccggcga
      661 tcacgaccag gtagccgcgc gaggcgtagg acgcggccgc cttctggttg gcggccacgc
      721 gcgggcaggt cgcgtcgacg acctcggcgc cggccgcccg gagccgctcg aagacctccg
      781 gggcggcgcc gtgggaccgt atgaccaccg tggcgccggc caggcgaccg ggagcgtcgt
      841 cggggtcggc tatcgtctcc atgcccaggc cggagagcct cgccgagacg cgggggttgt
      901 gcacgatctg gccgagcacg aagccgctcg agcccgaggc cgaagccgcc gcggcggccc
      961 gttccgccgt ctccaccgcc ctcctgacgc ccgggcagaa gccgagcgtg cgggccctcg
     1021 tcacggtcac cttcggtctc cctctcccta tgccagctcc agcgctatct ccatcatcgt
     1081 cttgaaggtg gtctgcctct cctcggcgct ggtcgcctcg cccgtcacga tgctgtcgga
     1141 cacggtcagg agcgtgagcg cccgcttgcg gaacttggcg gctatcgtgt atagcgcggc
     1201 ggtctccatc cccacggcca tcacgttgta
//
LOCUS       DAOOLN010000804         1228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_151412_length_1228_cov_2.334186, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000804 DAOOLN010000000
VERSION     DAOOLN010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151412_length_1228_cov_2.334186"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PLW80_09815"
     CDS             <1..601
                     /locus_tag="PLW80_09815"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPB66851.1"
                     /translation="AKEAHYSSILIVNESRAMYLEPALDAFGSPIRIQYGSKLTLYFY
                     SGNHVGYSFQSRSRGMVDIDGKKFLSIAHSDRIKPLPARRNQRSEVRLSGRFYLVHVR
                     AARDKGKVVKTVQVERAAVAGIITDLSGGGLSLQTMSPANVGEFIKVEFDLGTGQRSA
                     YATVVRVSKTRNGALMHLKFVKASRKTINEIRSVVYGYD"
     gene            713..973
                     /locus_tag="PLW80_09820"
     CDS             713..973
                     /locus_tag="PLW80_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66852.1"
                     /translation="MKIVELTNILRKETHIYYRREFKADAVVEIMQQTRNVPVEFVLE
                     HKPTGGVDVSATVVVDLDYPVMPFMSKLKLFISDLDKRGALP"
     gene            973..>1228
                     /gene="tsaE"
                     /locus_tag="PLW80_09825"
     CDS             973..>1228
                     /gene="tsaE"
                     /locus_tag="PLW80_09825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPB66853.1"
                     /translation="MRLECPDVESTERLGERIGEAAPSGTVIAFRGGLGAGKTAMCRG
                     IARGLGVSAPVTSPTYTLINEYDGERPFYHFDAYRLSSADE"
BASE COUNT          241 a          417 c          370 g          200 t
ORIGIN      
        1 ggccaaggag gcccactact cgtcgatcct gatagtcaac gagtcccggg ccatgtacct
       61 ggaaccagcc ctggatgcct tcggctcgcc gatacgcatc cagtacggct ccaagctgac
      121 gctgtacttc tactctggaa accacgtcgg ctacagcttc cagtcccgct cgcgcggcat
      181 ggtcgacatc gacggcaaga agttcctgtc catagctcac agcgaccgca tcaagccgct
      241 gcccgcgagg cgcaaccagc gtagcgaggt caggctatcg ggcaggttct acctggtgca
      301 cgtacgggcc gcgcgcgaca agggcaaggt cgtcaagacg gtgcaggtcg agcgggcggc
      361 ggtcgccggc atcatcaccg acctctcggg aggcgggcta tcgctccaga ccatgagccc
      421 ggccaacgtc ggagaattca tcaaggtgga gttcgacctg ggcaccggcc agcgctcggc
      481 ctacgcgacg gtcgtgcgcg tcagcaagac ccgcaatgga gccctgatgc acctgaagtt
      541 cgtcaaggcc tcgcgcaaga cgatcaacga gatacgctcg gtcgtctacg gctacgactg
      601 acgccgcccg gccgccgcgc ggacgtagcc gctcggcgtc ttgcgacgca tcccgtcgcc
      661 gcgcgacagg tcgcacttga ccgtcggagc caacgtacat acaatttcaa cgatgaagat
      721 agtcgagctc acgaatatcc tgcgcaagga aacgcatatc tactaccgaa gggaatttaa
      781 ggccgacgcg gtcgtcgaga tcatgcagca gaccaggaac gtcccggtcg agttcgtgct
      841 cgagcacaag cctaccggcg gcgtcgacgt gagcgccacc gtcgtcgtcg acctggatta
      901 cccggtgatg ccgttcatgt ccaagctcaa gctgttcatt tccgaccttg ataagcgcgg
      961 ggccctaccc tgatacgcct cgagtgcccc gacgtcgagt cgaccgagcg cctcggcgag
     1021 cggataggag aagcagcccc gtccggaacc gtcatagcgt tccgcggcgg gctcggcgcg
     1081 ggcaagaccg cgatgtgccg cggcatcgcc cgcggcctcg gcgtctccgc cccggtgacc
     1141 agccccacct ataccctgat caacgaatac gacggcgagc ggcccttcta ccatttcgac
     1201 gcctaccgcc tgtcgtcggc cgacgagt
//
LOCUS       DAOOLN010000805         1227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_151533_length_1227_cov_3.977816, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000805 DAOOLN010000000
VERSION     DAOOLN010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151533_length_1227_cov_3.977816"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLW80_09830"
     CDS             complement(<1..212)
                     /locus_tag="PLW80_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66854.1"
                     /translation="MKGKRDVPEMSNADLKRMLDQTMIRFENWRIEVERKPNPMMIHS
                     LKNIMSIIETVTSMADRRVEYSYKALV"
     gene            complement(392..>1227)
                     /gene="serC"
                     /locus_tag="PLW80_09835"
     CDS             complement(392..>1227)
                     /gene="serC"
                     /locus_tag="PLW80_09835"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HPB66855.1"
                     /translation="LGAGKEADYIVTGNWSQAAVKEAKRYGKITHPADMHLGDGMFVA
                     IPKQGELKLNPAAEYVHYTSNNTVEGTEWHYVPDVKGKPLVCDMSSDIFSRPVDFKKF
                     SMIYAGAQKNLGPSGVTVVAVKDDFLQKAHDDLPTMLSYKVFKEKFSMHNTPPCFSIY
                     ILNLVLRWLKKNGGLEAMGKINDQKQRLLYSAIDGSAGYFKGTANADSRSWMNVDFKL
                     PTPELDAKFVSDAKAAGIVQTKGYRSMGGIRLSCYNAVSLKDVETTVQFMQDFKAKNP
                     A"
BASE COUNT          208 a          330 c          400 g          289 t
ORIGIN      
        1 accagcgctt tgtaggagta ctccactcgg cggtcggcca tcgacgtgac cgtctcgatg
       61 atcgacatga tgttcttgag cgaatggatc atcatcgggt tgggcttgcg ctccacctcg
      121 atgcgccagt tctcgaagcg gatcatcgtc tggtcgagca tccgcttgag gtcggcgttg
      181 ctcatctccg gtacgtcgcg cttgcctttc atggtttcac ctgcctgaac ggagagaaga
      241 agaatcagac cacggaggca cggaggcacg aagggaaatg aggtcaagat agagcgcaaa
      301 ggaatatttt cctttctcat ctctcatcct ccattttctt cagctccgtg ccttcgtgtc
      361 tccgtggtaa cgtttttaag cttgcattat atcaggccgg gttcttggcc ttgaagtcct
      421 gcatgaactg cacggtcgtc tcgacgtcct tgaggctgac cgcgttatag caggacaggc
      481 ggatgccgcc catcgagcga tagcccttgg tctggactat cccggcggcc ttggcgtcgg
      541 agacgaactt ggcgtcgagc tcgggggtcg ggagcttgaa gtccacgttc atccaggagc
      601 gcgagtcggc gttggcggtg cccttgaagt agcccgcgga gccgtcgatc gcggagtaca
      661 ggagccgctg cttctgatcg ttgatcttcc ccatagcctc gaggccgccg ttcttcttga
      721 gccagcgcag cacgaggttc aggatgtaga tcgagaagca gggcggggtg ttgtgcatgg
      781 agaacttctc cttgaacacc ttgtaggaca gcatggtcgg caggtcgtcg tgggccttct
      841 ggaggaagtc gtccttgacc gcgaccacgg tgactccgga cgggccgagg ttcttctggg
      901 cgccggcgta gatcatgctg aatttcttga agtcgaccgg gcggctgaag atgtcgctcg
      961 acatgtcgca gacgagaggc ttgcccttga cgtcggggac gtagtgccac tcggtaccct
     1021 cgacggtgtt gttcgaggtg tagtggacgt actcggcggc cgggttgagc ttgagctcgc
     1081 cctgcttggg gatggcgacg aacatgccgt cgccgaggtg catgtcggcg gggtgggtga
     1141 tcttgccgta gcgtttggct tccttgacgg cggcctggga ccagttgccg gtgacgatat
     1201 agtcggcttc cttgcctgcg ccgaggt
//
LOCUS       DAOOLN010000806         1227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_151535_length_1227_cov_3.767918, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000806 DAOOLN010000000
VERSION     DAOOLN010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151535_length_1227_cov_3.767918"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLW80_09840"
     CDS             complement(<1..223)
                     /locus_tag="PLW80_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66856.1"
                     /translation="MDKLIVKGARENNLKNIDVELPRDKLIVVSGLSGSGKSSLAFDT
                     IFAEGQRRYVESLSAYARQFLGQMKKPDVD"
     gene            426..818
                     /locus_tag="PLW80_09845"
     CDS             426..818
                     /locus_tag="PLW80_09845"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPB66857.1"
                     /translation="MRLKQSWLVILIVVAVLALLGRITPALTEAAYAKYRASTRTFDV
                     SALADGVYEGSAFLFPVSVKARATVSGGRLASVELLRHFNGQGEPAEAIVGKVVEAQS
                     LGVDVVSGATHSSLTILKAVENALSRHP"
     gene            complement(958..>1227)
                     /locus_tag="PLW80_09850"
     CDS             complement(958..>1227)
                     /locus_tag="PLW80_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HPB66858.1"
                     /translation="RLLGLRSFFTSGKDECRAWTIRAGDKAPQAAGVIHTDFERGFIK
                     ADVYAYADIDALGSEAALRSAGKLRTEGKDYVVSDGDVMFFKFNV"
BASE COUNT          201 a          385 c          424 g          217 t
ORIGIN      
        1 agtcgacgtc gggcttcttc atctgcccga ggaactgccg ggcgtaggcc gacagcgact
       61 cgacgtagcg gcgctggccc tcggcgaaga tcgtgtcgaa cgccagggaa ctcttgccgg
      121 agcccgacag gcccgacacc acgatcaact tgtccctcgg caactcgacg tctatgttct
      181 tgagattgtt ctcgcgagcg ccttttacga tgagcttgtc catgatgcgt tctagcatat
      241 cgtcaccgcc gctaggacgg caagaggctt aacggttgtt tagtaattag gggcgccgac
      301 gatcgaggct atcgcgcggg ccgggcccgg cgtatactgg cgtccatcca tttcgactat
      361 gccgccggcc gggcccggcg tatactggcg cccgtccgaa cgggccgtac ggacggaggc
      421 ggagcatgag gctgaagcag agctggctgg tcatactgat cgtcgtggcg gtgctggcgc
      481 tgctcggcag gattacacca gcgcttaccg aggccgcgta cgcgaagtat agggcgtcga
      541 cccggacctt cgacgtatcg gccttagccg acggcgtcta cgaggggagc gccttcctct
      601 ttccggtgtc ggtcaaggcg cgtgcgaccg tctcgggcgg ccgcctggcc tcggtcgagc
      661 tgctcaggca cttcaacggc cagggcgagc cggccgaggc catagtcggg aaggtcgtcg
      721 aggcccagtc gctcggagtc gacgtcgtct ccggcgccac gcactcgagc ctgacgatac
      781 tcaaggccgt cgaaaacgcg ctatctcgtc atccatgacg tacccgtccg atgaagcgga
      841 cgggtcggcg ttgggccgcg cgaccctgcg cggcgaacgt ccgcgcgacc atcgaggagc
      901 gcgcgtccct gcgcgcacgg atggcgacgg cgttcgctcc gacgggcggc gcttactcta
      961 cacgttgaat ttaaagaaca tcacgtcgcc atccgagacg acgtagtcct tgccctcggt
     1021 gcgtagtttg ccggcggagc gcagggcggc ctcggagcca agcgcgtcga tgtcggcgta
     1081 ggcgtagacg tcggccttga tgaagccgcg ctcgaagtcg gtgtggatca cgccggcggc
     1141 ctggggggcc ttgtctccgg cgcggatggt ccaggcgcgg cactcgtcct tgccggaggt
     1201 gaagaaggag cgcaggccga gcaggcg
//
LOCUS       DAOOLN010000807         1227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_151644_length_1227_cov_2.127986, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000807 DAOOLN010000000
VERSION     DAOOLN010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151644_length_1227_cov_2.127986"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..>1227)
                     /locus_tag="PLW80_09855"
     CDS             complement(127..>1227)
                     /locus_tag="PLW80_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPB66859.1"
                     /translation="DILEAAVGVATERYGNPSSRHGAGRDARALLESARERLGAAIGA
                     APSSVVFTSGGSEADAIALLSTLVGKGERSVVISELEHPAVYEQARALEGLGVRVVRV
                     RPERDGVVSPGRVAEALRPDTAVVAVMAVSNETGAIQPIGRIVEAVRSAAGPRRPFVH
                     CDAVQALGTIGFDARALGVDGAAFSAHKLGGPRGVGALYLSRPISPLVLGGGQEGGMR
                     PGTHNTAGAWAFAEAAARAAASVEARMERARALERRLFDGLRAIPGATPLPEGRLPGD
                     ERYSPYIACLSFPGLAGETMTRLLDEAGVAISTGSACSSSKKERRSLDAMGVDRELSF
                     SSIRVSSGRDTSEADIDAFLEAASSAYIRFRV"
BASE COUNT          178 a          435 c          424 g          190 t
ORIGIN      
        1 gcttgagccg ctgctcgaat tcagtacggt tgccttcctt gagccgtatc tcgcctatct
       61 tgatcaggta tagcgcgtcc atcgcttcga tactcctatg gtcgataact tcaggctcgc
      121 gcgctcttag acgcggaagc ggatataggc cgacgaggcg gcctccagga aggcgtctat
      181 gtcggcttcg cttgtatccc tgccggacga cacgcgtatc gacgagaagg acagctcccg
      241 gtcgacgccc atcgcgtcga ggctcctgcg ctccttcttg gacgacgaac aggccgagcc
      301 ggtcgatatg gcgacgccgg cctcgtcgag gaggcgcgtc atcgtctcgc cggctagacc
      361 ggggaacgag aggcaggcta tgtaggggct gtagcgctca tcgccgggca gccgcccctc
      421 gggtagcggc gtcgcgcccg gtatggcgcg taggccgtcg aacaggcgcc gctcgagggc
      481 gcgggctcgt tccatgcgcg cctcgacgga cgcggcggcg cgggcggccg cctcggcgaa
      541 ggcccaggcg ccggcggtat tatgcgtacc gggcctcatg ccgccttcct ggccgccccc
      601 gaggaccagc ggactgatag gccggctcag gtacagcgct ccgacgccgc gcgggccgcc
      661 gagcttgtga gcgctgaagg ccgccccgtc gactccgagc gcgcgagcgt cgaagccgat
      721 ggtgccgagg gcttgcaccg cgtcgcaatg gacgaagggc cgcctcgggc cggcggccga
      781 acggacagcc tcgacgatgc gcccgatcgg ctggatagcg ccggtctcgt tgctcacggc
      841 catcaccgcc acgacggccg tatcgggccg cagagcctcg gccacccgcc cggggctcac
      901 gacgccgtcg cgctcgggcc gcacgcgtac caccctgacg ccgaggccct cgagggcgcg
      961 cgcctgctcg tagaccgccg gatgctcgag ttcggagatc accacggagc gctcgccctt
     1021 gccgacgagc gtcgatagga gcgctatggc gtcggcctcg ctaccgcccg aggtgaacac
     1081 gacgctcgaa ggcgccgcac ctatagccgc ccccaggcgt tccctggccg actcgagcag
     1141 ggctctggcg tcccgtccgg cgccgtgccg gctcgaaggg ttgccgtagc gttcggtcgc
     1201 tacgccgacc gcggcttcga gtatgtc
//
LOCUS       DAOOLN010000808         1227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_151650_length_1227_cov_2.011092, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000808 DAOOLN010000000
VERSION     DAOOLN010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151650_length_1227_cov_2.011092"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="PLW80_09860"
     CDS             <1..535
                     /locus_tag="PLW80_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB66860.1"
                     /translation="EYLRRFPHELSGGQRQRVNIARTFAVEPSIVLADEPTSMLDVSI
                     RMGVMNMMLDLKERKGVSYLYITHDLAGARYMCGSIAVMYAGMIMEMGPTDEVIRDAV
                     HPYVRLLKSASPSPEAGLVRTPLDAGGEIPSLIDPPPGCRFHPRCPYAEPRCRESVPG
                     LADLGGGRRVRCVLALR"
     gene            complement(532..1170)
                     /locus_tag="PLW80_09865"
     CDS             complement(532..1170)
                     /locus_tag="PLW80_09865"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HPB66861.1"
                     /translation="MNLLAHVLLSWATLPDTEGQECTGALMADYFPGQDLAVFPPGIR
                     RGILQHRAVDAFTDVHPAFVELRRSIAAAGAPRFTAGILLDVFWDHALASEWERWGRQ
                     LCGMDLEPFCGEAYARVGRAAEYHNPGFASIFRFLESGAWLSAYARLDGIGETLAMLS
                     EKMSGRPDLGSCVRLLVELDRPVRSGFAAFWPELLAFARAWNDDAPVEALRS"
BASE COUNT          206 a          414 c          438 g          169 t
ORIGIN      
        1 ggagtacctg cggcgcttcc cgcacgagct gtcgggcggg cagcggcagc gggtgaacat
       61 cgcgcgcacc ttcgccgtag agccgtccat cgtgctggcc gacgagccga cgagcatgct
      121 cgacgtgtcc atacgcatgg gcgttatgaa catgatgctc gaccttaagg agcgcaaggg
      181 cgtctcatac ctgtacataa cccacgacct ggccggcgcg cgctatatgt gcggctccat
      241 cgcggtgatg tacgcgggca tgatcatgga gatggggccc accgacgagg tcatccggga
      301 cgccgtgcac ccctacgtac ggttgctcaa gtcagcctcg ccctctccgg aggccggcct
      361 ggtccggacc ccgctcgacg cagggggcga gataccgtcg ctgatagacc cgccgcccgg
      421 atgccgcttc catccgcgct gcccctacgc cgagccgcgc tgccgcgagt cggtgcccgg
      481 gctggccgac ctgggcggcg ggcgcagggt gcgttgcgta ctggccctgc gctaggaccg
      541 gagggcctcg accggggcgt catcgttcca ggcccgagcg aaggcgagca gctccggcca
      601 gaacgcggcg aagcctgagc gaacgggacg gtccagctcg acgagtagcc gcacgcagct
      661 gcccaggtcg gggcggccgc tcatcttctc ggacagcatg gccagggtct cgccgatgcc
      721 gtcgagcctg gcgtaggctg acagccacgc ccccgactcc aggaagcgaa agatggaggc
      781 gaagcccgga ttatggtact cggccgcccg accgaccctg gcgtaggcct cgccgcagaa
      841 cggctcgaga tccatgccgc agagctgacg gccccagcgt tcccactcgg aggcgagcgc
      901 gtggtcccag aacacgtcga ggagtatacc ggcggtgaag cgcggcgcgc cggccgcggc
      961 gatggagcgg cgcagctcga cgaaggcagg gtggacgtcg gtgaacgcgt cgacggcgcg
     1021 gtgctggagt atgccgcgac gtatgcccgg cggaaagacg gccagatcct gcccggggaa
     1081 gtagtcggcc atcagcgcgc cagtacattc ctgcccctcg gtgtcgggga gggtagccca
     1141 ggagagcagt acgtgcgcga gcaggttcac agatggcctc cgatccggcc atcatagccg
     1201 ccgacccggg ccggcggcaa gcctggc
//
LOCUS       DAOOLN010000809         1226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_151818_length_1226_cov_2.411614, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000809 DAOOLN010000000
VERSION     DAOOLN010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151818_length_1226_cov_2.411614"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..>1226)
                     /locus_tag="PLW80_09870"
     CDS             complement(138..>1226)
                     /locus_tag="PLW80_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66862.1"
                     /translation="ERTDEMKRLSRFAAVVATAVAAGMAAASCASIQRDVYYDSPAGE
                     DPAELAELESSLVRSRAVPGAGNLAAARARLAELASAPSSDSARSARILALSAEAAIQ
                     SGDRNAAARLLAASKAAYRGDEVAAVVESRLAPTQAERLAVLDRSAAVADGGYRVGAE
                     RGSALLALGRIREAIVAFDAALPRLGEEYALLFGDERERAWSLRDAESAPSADASAYL
                     TRVPITLLGMAAVAQSETTAMDRVTGGAPWAAGVLFERLKAAGWYIDSGADAKSPATR
                     KDAAMFLWTMMCRGDRAMAARYTARYASRGRSPVPDVPLGSPYFDAALGVVEEGVMAL
                     VDGSAFDPDGPASGLDFYGWLLAAAAWR"
BASE COUNT          181 a          461 c          438 g          146 t
ORIGIN      
        1 cgtagtctcg cggcgcgagg agaagaaggt ccgcgccggt gaagacgcca agcctggcca
       61 ggcgcttaca gaccgacggc ccggcaccct tgaggtcgga taccggcgcc cctatttcgc
      121 ggaggaacaa cgccgcccta gcgccaggcc gcggccgcga gcagccagcc gtagaaatcg
      181 aggcccgagg ccgggccgtc cgggtcgaag gcgctaccgt cgaccaaggc catcacgccc
      241 tcctccacca cgccgagggc cgcgtcgaag tacggcgagc cgaggggcac gtccggcacg
      301 gggctacggc cccgcgaggc gtagcgggcc gtatagcgcg ccgccatcgc ccggtcgccc
      361 cggcacatca tcgtccagag gaacatcgcc gcgtccttcc tcgtggccgg ggacttggcg
      421 tctgcgcccg agtcgatata ccagccggcc gccttgagcc gctcgaacag cacaccagcg
      481 gcccatggcg ccccgccggt gacgcggtcc atcgcggtgg tctccgactg ggcgaccgcc
      541 gccatgccga gcagagtgat gggtacgcgc gtcaggtacg ccgaggcgtc ggccgacggc
      601 gccgactcgg cgtcgcgcag cgaccaggcc cgctcgcgct cgtctccgaa caggagggcg
      661 tactcctcgc cgagcctggg cagggccgcg tcgaaggcaa cgatggcctc gcggatgcgc
      721 ccgagcgcca ggagggccga gcccctctcg gccccgacgc gatagccgcc atcggcaacg
      781 gccgccgagc gatcgaggac cgcgagccgc tcagcctggg tcggagccag gcgggactcg
      841 acgacggctg cgacctcgtc gccccggtac gccgccttgg aggcggccag cagcctggcg
      901 gccgcgttcc ggtccccgga ctggatggcg gcctcggccg acagcgcgag tatcctcgcg
      961 ctcctggccg agtcgctcga cggcgcggac gcgagctccg cgaggcgggc tcgggccgcg
     1021 gccaggttgc cggccccggg caccgccctg gaccgcacga ggctggactc gagttcggcg
     1081 agctcggcgg gatcctcccc ggccggcgag tcgtagtaca cgtcgcgctg gatcgaggcg
     1141 caggaagcgg cggccatgcc ggccgcgacg gccgtcgcca ctacagccgc gaatctggac
     1201 aatctcttca tctcgtccgt cctttc
//
LOCUS       DAOOLN010000810         1223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_152336_length_1223_cov_3.942637, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000810 DAOOLN010000000
VERSION     DAOOLN010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152336_length_1223_cov_3.942637"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..>1223
                     /locus_tag="PLW80_09875"
     CDS             138..>1223
                     /locus_tag="PLW80_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66863.1"
                     /translation="MRRRMLIEENLLISAALSIATLVVVLGSFFLIAGRHEIDGIRLQ
                     SEATADEIASILIEPLYNIDDNQAARIAAALVASGRIDSLRLESTVSGVIFEQRAATP
                     PRWIQAQDRRIERGELHLGDVRIAVSEREIRETLSLYMLASGLLAVATVIVNATVNLF
                     FIRRRVRKAFVDFEEGIRQINGGAFGYEIPPSGYHDLDHLILSFNRMSRSVALSNAQL
                     SSLNAELEERVTERTSELRTALAEQDKLRERLVEAAKMSVLGRLSAGVAHEINTPLGA
                     IVSSNGYLIEFIEDEYRSQLEYFAGLPALEREAYERLLTIGVAGNRGLSASMASSAER
                     RSFAEALSSAGVPDAEAIADELADLSLH"
BASE COUNT          236 a          403 c          381 g          203 t
ORIGIN      
        1 cgcgacctcg acgcgctaat ggcccgcttc gccaggttca ggacctgacg agaccgccgc
       61 gcaggggctc ttgcgccaga cgtccggcga catatagtta taaagaaagc ctttaacgcc
      121 gaggagccaa ctccgccatg agacgccgca tgctgataga agagaacctg ctcatctcgg
      181 cggccctgtc gatcgcgacc ctggtggtcg tgctcggctc gttcttcctg atagccggcc
      241 ggcacgagat cgacggcata cggctacagt ccgaggcgac ggccgacgag atagccagca
      301 tcctgatcga gccgctctac aatatcgacg acaaccaggc cgccaggata gcggcggccc
      361 tcgtcgcgtc cgggcgcatc gacagccttc gtctggaatc gacggtctcc ggcgtcatat
      421 tcgaacagag ggcggctacc cccccgcgat ggatacaagc ccaggatcgg cgcatagagc
      481 gcggcgagct acacctcggc gacgtacgta tcgcggtaag cgagcgagag atacgcgaga
      541 cgctatcgct ctacatgctc gccagcggcc tgctcgcggt cgcgaccgtc atcgtgaacg
      601 ccacggtcaa cctgttcttc atacgcaggc gcgtccgtaa ggcgttcgtc gacttcgagg
      661 aagggattcg acagataaac ggcggagcgt tcggctacga gatacctccg agcggctatc
      721 acgaccttga tcacctgatc ctctcgttca ataggatgag ccgaagcgtc gcgctctcca
      781 acgctcagct ttcaagcctg aacgccgagc tcgaggagcg ggtgaccgag cgcacctcag
      841 agctccggac ggcgctggcc gagcaggaca agcttcgcga gcgcttggtc gaggccgcca
      901 agatgtcggt gctcggacgg ctatcggccg gcgtggccca cgagataaac acgccgctcg
      961 gcgccatcgt gtcgtcgaac ggctacctga tcgagttcat cgaggacgag taccggtctc
     1021 agctcgagta ctttgcggga ttgcccgcct tggaacgcga ggcctacgag cggctcctga
     1081 ccatcggagt agcgggcaac cgcggcctgt ctgccagcat ggcatcgtcc gccgagaggc
     1141 gctccttcgc cgaggcgcta tcctcggcgg gggtccccga cgccgaggcc atagccgacg
     1201 agctggccga cctgtcgctc cac
//
LOCUS       DAOOLN010000811         1223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_152370_length_1223_cov_2.797089, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000811 DAOOLN010000000
VERSION     DAOOLN010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152370_length_1223_cov_2.797089"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLW80_09880"
     CDS             complement(<1..320)
                     /locus_tag="PLW80_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66864.1"
                     /translation="MRAFALFYKNFMIGAFRNKLAFLVYFAAPIASFVAMFFLLRLTG
                     DESFAAAQAIGLVVYFSMLQATMITSLTIRDREEGAALRIAASPTGGLAYAIGNGAAA
                     FTVLG"
     gene            complement(317..1054)
                     /locus_tag="PLW80_09885"
     CDS             complement(317..1054)
                     /locus_tag="PLW80_09885"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB66865.1"
                     /translation="MVTLVFARHQVQLFLKSPGVMALFIVGPLFVIFVFGQAFTAIFA
                     AGGQGFDAMQYFGVTLLTMAVFQGSFIASWAVFKERRSNTEVRLGVAPIGRLQATLGA
                     FLGSWLSLAALGCAVMALASVLLSIDYGERLWLSALLVCAEALLAASLGVSLAVIFGE
                     ERAANGVMNALVPALVFIGGGYTIIPESGFLHELARFSPLRWINLAMLDLAMPGPGEY
                     IAAALTFSLTASAALMTLACLRLWRNR"
BASE COUNT          243 a          373 c          423 g          184 t
ORIGIN      
        1 ccgagcaccg tgaaggccgc ggcgccattc ccgatggcat aggcgaggcc acctgtcggc
       61 gaggccgcga tgcgtagggc tgcgccctct tccctgtccc tgatggtcag gctggttatc
      121 atcgtggcct ggagcatcga gaagtagacg acgaggccga tggcctgggc ggcggcgaac
      181 gactcgtcgc cggtgaggcg aagcaggaag aacatcgcca cgaagctggc gatcggagcg
      241 gcgaagtaga cgaggaaggc cagtttgttc ctgaaggccc cgatcatgaa gtttttatag
      301 aacagcgcga aggctctcat cggttcctcc atagcctgag gcaggcgagc gtcatcagcg
      361 cggcggaagc cgtcaggctg aaggtcaggg cggcggcgat gtactcgccg ggacccggca
      421 tggccaggtc gagcatggcc aggttgatcc agcgtagtgg cgagaagcgc gccagctcgt
      481 gcaggaagcc gctctccggt atgatcgtat agccgcctcc gatgaagacg agcgccggca
      541 cgagcgcgtt catcacgccg ttggccgccc tctcctcgcc gaagatgacc gccagcgaga
      601 cgccgaggct cgccgccagc aaggcctcgg cacagacgag cagcgccgaa agccagagcc
      661 gctcgccgta gtcgatcgac aggagtaccg aggcgagcgc catgactgcg cagccgagcg
      721 ccgcgagcga cagccacgag ccgaggaagg cgccgagcgt cgcttggagg cggccgatgg
      781 gcgccacgcc gagccgaacc tcggtgttcg accgcctttc cttgaagacg gcccacgagg
      841 ctatgaagga gccctggaac acggccatcg tgagcagggt gacgccgaag tactgcatgg
      901 cgtcgaagcc ctggcccccg gcggcgaaga tggcggtgaa ggcctggccg aagacgaaga
      961 taacgaagag cgggccgacg atgaacagcg ccatcactcc tgggctttta aggaacagct
     1021 gtacctggtg cctggcgaat actagggtta ccatgtcgtc cacctagtcc cgcagggaac
     1081 ggccggtaag gctcaggaat accgtctcga gcgatggcga gtcggtctca atatcggtga
     1141 cgatgagtcc ctgccggttg acctcggctg tcagctcgtt caggttggtc gcggacctcg
     1201 ctggtcacgc tcaggcgcag ctc
//
LOCUS       DAOOLN010000812         1222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_152517_length_1222_cov_3.422451, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000812 DAOOLN010000000
VERSION     DAOOLN010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152517_length_1222_cov_3.422451"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /locus_tag="PLW80_09890"
     CDS             complement(<1..132)
                     /locus_tag="PLW80_09890"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HPB66866.1"
                     /translation="MAIYVLENTVQHYAWGSNDALPALLGKENPSGEPWAELWMGAHP
                     "
     gene            complement(157..678)
                     /locus_tag="PLW80_09895"
     CDS             complement(157..678)
                     /locus_tag="PLW80_09895"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HPB66867.1"
                     /translation="MLFTSYTALRAAWDAVKPRLDALGISAFRQGDDERSRLLGSFVA
                     DVSSVLFATDSFWEGIDAPGETLELVIITKLPFRVPTDPVQKARSEDIERRGGNSFMD
                     LSLPEAVIRFKQGFGRLIRHSNDRGAVVVLDVRLLTKRYGRVFVSSLPRTRTRFGPLA
                     EVAGEVRSFIGRP"
     gene            complement(705..>1222)
                     /locus_tag="PLW80_09900"
     CDS             complement(705..>1222)
                     /locus_tag="PLW80_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66868.1"
                     /translation="LGELERALLSMAELIKDALAGVPDESSEDQSVREAELALVRLSE
                     LAAVCARYRSFDEEPNEIFWLERRKTSQGEGFAQLMITPLDISFMMNEAVFDQFRTVA
                     CLSATLSIGGSFGFWKNRVGLSASGARVECREYPSPFPFERNALLAATSDAPDPSSPS
                     GKLGSTTPSTS"
BASE COUNT          205 a          397 c          426 g          194 t
ORIGIN      
        1 tggatgggct cccatccata gctcggccca cggctcgccg ctcgggttct ccttgcccag
       61 cagcgccggc agggcgtcgt tcgagcccca ggcgtagtgt tgtacggtgt tctcgagtac
      121 gtagatggcc atggggtatt gtaccccgac tgtcgctcac ggtctaccga tgaacgaacg
      181 gacctccccc gcgacctcgg cgagcggtcc gaagcgggta cgggtacggg gtagcgacga
      241 tacgaagacg cggccgtagc gcttggtcag gagccgcacg tcgagcacga cgacggcgcc
      301 gcggtcgttc gagtggcgga tcagccggcc gaagccctgc ttgaagcgta tgacggcctc
      361 cggtagcgat aggtccatga aggagttgcc gccccgccgc tcgatatctt cggatcgggc
      421 cttctggacc gggtcggtcg gcaccctgaa cggcagcttg gtgatgatga cgagctcgag
      481 cgtctcgccc ggcgcgtcta tgccctccca gaacgagtcg gtcgcgaaca ggacgctcga
      541 gacgtcggcg acgaaggagc cgagcagcct ggagcgctcg tcgtcgccct gcctgaaggc
      601 ggagatgccg agcgcgtcga gccgcggctt caccgcgtcc caggccgcgc gcagggccgt
      661 atacgaggtg aacagcacga gggcgcggcc gcccgaggcc gcgatcagct cgtagacggc
      721 gtcgttgacc caagcttgcc agagggcgac gatgggtccg gggcgtcgct ggtcgcggcg
      781 agcagggcat tgcgctcgaa ggggaagggc gaggggtact cgcgacactc gactctggcg
      841 ccggaagccg ataatccgac ccggttcttc cagaatccga acgagccgcc gatggaaagc
      901 gtcgccgaca ggcaggcgac ggtgcggaac tggtcgaaca ccgcctcgtt catcatgaag
      961 gaaatgtcga gcggggtgat catcagctgg gcgaagccct cgccctggct ggtcttccgc
     1021 ctctcgagcc agaagatctc gttcggttcc tcgtcgaagc ttcggtagcg cgcgcagacg
     1081 gccgccagct ccgacagcct gaccagggcc agctcggctt cgcgtacgga ctggtcctcg
     1141 gacgactcgt ccggtacccc ggcgagcgcg tccttgatca gctcggccat cgacaggagc
     1201 gccctctcga gctcgccgag cg
//
LOCUS       DAOOLN010000813         1221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_152774_length_1221_cov_2.297599, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000813 DAOOLN010000000
VERSION     DAOOLN010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152774_length_1221_cov_2.297599"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PLW80_09905"
     CDS             complement(<1..260)
                     /locus_tag="PLW80_09905"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPB66869.1"
                     /translation="MKYKIGSFRRWTGLGEGALRYYEDLGLISPYRDVGNDYRYYDER
                     NFLQLVQVRQLSGFDIQLGELPRVDTTVPIEDMRAILSRRRAA"
     gene            591..>1221
                     /locus_tag="PLW80_09910"
     CDS             591..>1221
                     /locus_tag="PLW80_09910"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPB66870.1"
                     /translation="MSKRRVAPLALLAVAMLAVIGGCATGTARNGEAPVSGTYEGTGY
                     GMQGPIKVQVTVDGGKIAAVKILSSKETPNVVKVAFERLPAQIVEHQALGLDAVCGAT
                     LASNGIKNAVADAVKKAGMDATKLREKTYKPQPKAPQTWSADVLVMGAGGAGLTAAIS
                     AAQTGASVILIEKGSVLGGNTMMAGAAYNAVDPAAQAMMTLSKAQKQTMD"
BASE COUNT          271 a          363 c          365 g          222 t
ORIGIN      
        1 gccgctcgcc tccgggacag tatcgcgcgc atatcctcga tgggcacggt cgtatctacc
       61 cgagggagct cgccaagctg gatatcgaag ccgctcagtt gccgaacctg caccagctgc
      121 aggaagtttc gttcatcgta atacctatag tcgttgccga catcgcggta cgggctgatc
      181 aagccaagat cctcgtagta ccgcagcgcc ccctcgccca atccggtcca tcgcctgaac
      241 gaacctatct tgtacttcat cgcgttgctc catgtttcaa tgtagtcgtc cataaccccg
      301 tagccgcttt aaggttcgcc gtaaagccag accgcgcgat ccgagccgga ccatgcggcg
      361 tgatcacgaa caccgaagcc ggcagtagca tctcgataca ttataatgct ttaggcatcg
      421 actgtctgct ctatgccgac tcgcgatcaa tagtcgcgat caagtagaag cgacctgtcg
      481 accgtaggat ccgattataa aagatattta tatattttta gagattgacc ctacagtcgc
      541 ctacgggtgt accgtttcgg atggggtatt cccctatgga ggtatgcacg atgagcaaga
      601 gaagggtagc gccgttagcg cttctggccg tggccatgtt ggcggtcata ggcggatgcg
      661 ccaccggtac ggctaggaac ggcgaagcgc cggtatctgg aacctatgaa ggtactggct
      721 atggaatgca gggtcccatc aaggtacagg tcacggtgga tggcgggaag atagccgccg
      781 tcaagatact gagcagcaag gaaacgccga acgtcgtcaa ggtagcgttc gagcgcctcc
      841 ccgcccagat cgtggagcac caggcgctcg gcctggacgc ggtctgcgga gcgacgctgg
      901 ccagcaacgg catcaagaac gccgtcgccg acgctgtcaa gaaagccggc atggacgcga
      961 cgaagctccg ggagaaaacc tataagcccc agcccaaggc tccccagacc tggtcggccg
     1021 acgtactggt catgggagcc ggaggagccg ggctcaccgc ggccatcagc gccgcccaga
     1081 ccggcgcctc ggtcatactg attgagaagg gctccgtcct gggcgggaac accatgatgg
     1141 ccggcgccgc gtataacgcc gtcgacccgg ccgcgcaggc catgatgacg ctgagcaagg
     1201 cgcagaagca gacgatggac g
//
LOCUS       DAOOLN010000814         1221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_152807_length_1221_cov_1.959691, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000814 DAOOLN010000000
VERSION     DAOOLN010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152807_length_1221_cov_1.959691"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PLW80_09915"
     CDS             <1..559
                     /locus_tag="PLW80_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tetrahydrofolate synthase"
                     /protein_id="HPB66871.1"
                     /translation="LPARLATPMIGSVQLRNAALAALAGASIGLTRDDIVRGLSAARL
                     RARFEILDAGTRVILDGAHTDDSIRACVDDFSSLFPGGGSLLFGCAADKAAGAMAESL
                     RSAFTEVVITRPGTFKESDTAALERTFIEHGFEVRRIDDTAEAVRTAIDGAIAAGLPL
                     LVTGSFYLCAYAAAELEARRARHQA"
     gene            complement(556..>1221)
                     /locus_tag="PLW80_09920"
     CDS             complement(556..>1221)
                     /locus_tag="PLW80_09920"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPB66872.1"
                     /translation="IRLQAQTKLMQEREYYRQAVRQEENLTIKLLDRQLGLKETLADL
                     ESKKAGLERENTGLAAAARFDVLTSLLNRHGLNARLELEARRSDAEDARLSGLMVDVD
                     RFKAVNDSYGHLVVEEALRSVGDALRACLRKEDFAGRYGGDEFFAILPGSGIEAALAI
                     AERIRSTIAAAEIHLGGVRVRLSASIGAAERRKGDSVAEWIGRADAAMYRAKNAGRDR
                     VMS"
BASE COUNT          183 a          433 c          397 g          208 t
ORIGIN      
        1 cctgcccgcc aggctggcga cgcccatgat aggctcggtg cagctcagga acgcggcgct
       61 ggcggccttg gcgggcgcct cgatagggct aacccgcgac gatatcgttc gcggcctaag
      121 cgccgccaga ctccgcgccc gcttcgagat actcgacgcc ggcacccgcg tcatcctgga
      181 cggcgctcac accgacgact cgatccgggc ctgcgtcgac gacttctcga gcctcttccc
      241 gggaggaggc tccctgctgt tcgggtgcgc cgccgacaag gcagccggag ccatggccga
      301 atcgctacga tcggcgttca cggaggtcgt catcacgcgc cccggcacct tcaaggagag
      361 cgacaccgcc gctctcgaga gaacctttat cgagcatggc ttcgaggtgc ggaggatcga
      421 cgatacggcc gaggccgtac gaacggcgat cgacggggcg atcgccgccg ggctaccgct
      481 cctggtgacc ggttcgttct acctgtgcgc gtacgcggcc gccgagctcg aggccaggcg
      541 agcccggcac caggcctagc tcatcacccg gtcgcggcca gcgttcttgg ccctgtacat
      601 ggcggcgtcg gcgcggccga tccattcggc caccgaatcg cccttacgtc gctcggccgc
      661 gccgatcgat gcgctgaggc gtaccctgac tccgccgagg tgtatctccg cggccgcgat
      721 cgtcgagcgt atgcgttcgg ctatcgccag cgccgcttcg atgccgctgc cgggcaggat
      781 cgcgaaaaac tcgtcgccgc cgtagcgccc cgcgaagtct tccttgcgga ggcaggcgcg
      841 tagcgcgtcg cccacggagc gtagcgcctc ctcgacgacg agatggccat acgagtcgtt
      901 gacggccttg aaccggtcca cgtcgaccat gaggccggac agccgcgcgt cctcggcgtc
      961 ggagcggcgc gcctcgagct cgagcctggc gttcaggccg tgccgattga ggagactcgt
     1021 cagcacgtcg aagcgggcgg cagcggctag cccagtgttc tcgcgctcaa gccccgcctt
     1081 cttgctctcg aggtcggcta gcgtctcctt gaggccgagt tgcctgtcga gcagcttgat
     1141 agtcaggttc tcctcctgac gtaccgcctg acggtaatac tcacgctcct gcatcaactt
     1201 cgtctgcgcc tggagccgta t
//
LOCUS       DAOOLN010000815         1220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_152941_length_1220_cov_2.622318, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000815 DAOOLN010000000
VERSION     DAOOLN010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152941_length_1220_cov_2.622318"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..>1220)
                     /locus_tag="PLW80_09925"
     CDS             complement(176..>1220)
                     /locus_tag="PLW80_09925"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HPB66873.1"
                     /translation="RLDGIEVINPATGKVVPIYGALSGAQNVAVVDVTMTWDKEAKTW
                     KKDIAGSLVPLKGVAADPAFLAKFEPAKAELMDWVERPIGKMVGSINSDEAMFGDSAF
                     VDLINRIQLELCADATMGLKPAQISIAAPLDQYAYLPTMQDGTIKVRDMFSLYKYENF
                     MYTMDLTGEQIVKFMEFNYGNQFNTMTGPQDQLIAFAKDKEGKPVYNARYNNYDTVTR
                     PYNYDAVAGIKYTVDVSKPAGSRIKVISMADGSPFELGRTYSVAINSYRGSGGGGHLT
                     KGVGLPAEKLQKLELVTSSTTKDLRFYMLKWFEKQTGPITVTRDDNWKVIPEDWAEAG
                     KANSYPLMYPPKK"
BASE COUNT          188 a          352 c          423 g          257 t
ORIGIN      
        1 ccgtacggag cgggcgttgc ggacccgagg gcgtccgcgg gccggtgcgg ctatcgccgt
       61 gccggtatac gtacatgata cgaacgcttt ccttaaaagg caagcccgga tcccatggcg
      121 gggtccgggc ttgcctgccg acggggctac agcccgccgg ccgttcggga gaccgttact
      181 tcttgggcgg gtacatcagc gggtaggagt tggccttgcc ggcctcggcc cagtcctcgg
      241 ggatgacctt ccagttgtcg tcacgggtga ccgtgatggg gccggtctgc ttctcgaacc
      301 acttgagcat atagaagcgc aggtccttcg tggtgctcga ggtgaccagt tccagcttct
      361 ggagcttctc ggcagggagg ccgacgccct tggtcaggtg gccgccgccg cccgagccgc
      421 ggtacgagtt gatggcgacc gagtaggtcc taccgagctc gaagggcgag ccgtcggcca
      481 tcgagatgac cttgatccgg gaaccggcgg gcttggacac gtcgaccgtg tacttgatgc
      541 cggcgacggc gtcgtagttg tagggccggg tgaccgtgtc gtagttgttg taacgggcgt
      601 tatagaccgg cttgccttcc ttgtccttgg cgaaggcgat gagctggtcc tgcggtccgg
      661 tcatggtgtt gaactgattg ccgtagttga attccatgaa cttgacgatc tgctcgccgg
      721 tcaggtccat cgtgtacatg aagttctcgt acttgtacag gctgaacatg tcgcgcacct
      781 tgatggtgcc atcctgcatg gtcggcagat aggcgtactg gtcgaggggg gcggctatcg
      841 atatctgggc cggcttgaga cccatggtcg cgtcggcgca tagctcgagc tgaatgcggt
      901 tgatcaggtc gacgaaggcc gagtcgccga acatcgcctc gtcgctgttg atcgagccga
      961 ccatcttgcc gatcgggcgc tcgacccagt ccatcagctc ggccttggcg ggctcgaact
     1021 tggcgaggaa cgccgggtcg gcggccacgc ctttgagcgg cacgagcgag ccggcgatgt
     1081 ccttcttcca ggtcttggct tccttgtccc aggtcatggt cacgtcgacg acggccacgt
     1141 tctgggcgcc ggagagggcg ccgtagatcg gcacgacctt gccggtcgcg gggttgatga
     1201 cttcaatgcc gtcgaggcga
//
LOCUS       DAOOLN010000816         1220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_153026_length_1220_cov_1.787124, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000816 DAOOLN010000000
VERSION     DAOOLN010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153026_length_1220_cov_1.787124"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="PLW80_09930"
     CDS             <1..1091
                     /locus_tag="PLW80_09930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPB66874.1"
                     /translation="MVTKTRIIDTFFPVARGGTYCIPGPFGAGKTVLQQVTSRNADVD
                     IVIIAACGERAGEVVETLKEFPELLDPRTGKSLMERTIIVCNTSSMPVAAREASVYTA
                     VTMAEYYRQMGLNVLLLADSTSRWAQAMREMSGRLEEIPGEEAFPAYLESVIASFYER
                     AGIVRLNDGRLGSVTIGGTVSPAGGNFEEPVTQATLKVVGAFHGLSRERSDARRYPAI
                     HPLDSWSKYPSLIPEDQLMLARRMLRDGNDVNQMMKVVGEEGTAIEDFIVYLKSEYID
                     AVYLQQDAYDEVDGATPADRQKHMFERLYSVLMARMHFKDKAGARKFFQALTQATKDW
                     NRIPMDSDEFKAAERRITQMLAEVTPNA"
BASE COUNT          232 a          407 c          395 g          186 t
ORIGIN      
        1 cgatggtcac gaagacgcgc atcatcgata ccttcttccc ggtcgcccgg ggcggcacct
       61 actgcatccc cggcccgttc ggcgccggca agacggtact gcagcaggtg acgagccgca
      121 acgccgacgt cgacatcgtc atcatcgccg cctgcggcga gcgcgccggc gaggtcgtcg
      181 agacgctcaa ggaattcccc gagctcctcg acccgcgcac cggcaagtcg ctgatggaac
      241 gtacgatcat cgtctgcaac acctcgtcga tgccggtcgc ggcgcgcgag gcctcggtgt
      301 ataccgcggt gacgatggcc gagtactacc ggcagatggg cttgaacgtg ctgctcctgg
      361 ccgattcgac gagccgctgg gcccaggcca tgcgcgagat gtccggccgc ctcgaggaga
      421 tccccggcga ggaggccttc ccggcctacc tggagtcggt gatcgccagc ttctacgagc
      481 gcgccggcat cgtgaggctc aacgacggcc gcctcggctc ggtcaccatc ggcggcaccg
      541 tgagcccggc cggcggcaat ttcgaggagc cggtgaccca ggcgacgctc aaggtggtcg
      601 gcgcgttcca cggcctgtcc cgcgagcgct ccgacgcgcg gcggtacccg gccatccacc
      661 cgctggacag ctggagcaag tacccgagcc tcatcccgga agaccagctt atgctggccc
      721 ggcgcatgct gcgcgacggc aacgacgtga accagatgat gaaggtcgtg ggcgaggaag
      781 gcacggcaat cgaggacttc atcgtgtatc tcaagtccga gtacatagat gcggtctacc
      841 tccagcagga cgcctacgac gaggttgacg gcgccacgcc ggccgaccgc cagaagcaca
      901 tgttcgaacg cctttactcg gttctcatgg cgcggatgca tttcaaggac aaggccgggg
      961 ccaggaaatt cttccaggcg ctcacgcagg caacgaagga ttggaaccgc atccccatgg
     1021 actcggatga gttcaaggcc gccgaacgca ggataacgca aatgctcgcc gaggtgacgc
     1081 ccaatgcgta gaatatacaa tcgcgtcgaa cggatagccg gcaacgtcat aagcgtgcgg
     1141 gcccagaacg tcagctaccg ggagctggcc caggtgtcct ccggccgggg cgtgtccctg
     1201 gcccaggtca tccggctgca
//
LOCUS       DAOOLN010000817         1218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_153318_length_1218_cov_3.411866, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000817 DAOOLN010000000
VERSION     DAOOLN010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153318_length_1218_cov_3.411866"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PLW80_09935"
     CDS             complement(<1..314)
                     /locus_tag="PLW80_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66875.1"
                     /translation="MTPLIIALAAQAVVSVALIAWTRVKVRRFLGADEELERIRREIG
                     SLIVELDATADRNVTVLEDRLRALKDSLAEADKRIALLGQERSRRQAEAVVYDRLGRA
                     GEP"
     gene            complement(318..641)
                     /locus_tag="PLW80_09940"
     CDS             complement(318..641)
                     /locus_tag="PLW80_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66876.1"
                     /translation="MPIKPIDLQTLFLQLSQVGKEQAAAKDGALLHANLHGTVVKRLE
                     DEAAKAVQRPKDDQAAAKSVNGDGGSGVGSQGGERRREETPAGDDEAETISDPSLGGH
                     VDISG"
     gene            complement(622..>1218)
                     /locus_tag="PLW80_09945"
     CDS             complement(622..>1218)
                     /locus_tag="PLW80_09945"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HPB66877.1"
                     /translation="RADLAQSAKAQSGQKHAAIIGGRYRACEEINAKSIGSPSGGAET
                     ILEVGSDPKSKLKMDELDGKMKLLQRQIDELDKNIYTLNETKRQRKTLSDDKQAVLDE
                     LTHRRDELKQESSSLKDEYDALQAYLNNLKVRGKVSVSGRIYPGSEIVIRDIREKIKN
                     EYKGLTFYLENMMIKTTRYEEFDDEILRKGPPDAHKAD"
BASE COUNT          163 a          395 c          392 g          268 t
ORIGIN      
        1 ggctctccgg cacgaccgag cctatcgtag acgaccgcct cggcctgccg cctggaccgt
       61 tcctggccga ggagggcgat acgtttgtcg gcctcggcga gcgagtcctt gagggcccgg
      121 agccggtcct cgagcaccgt gacgttccgg tcggcggtcg cgtcaagctc gacgatcagg
      181 gaaccgatct cgcgccgtat gcgctccagc tcctcgtctg cgccgaggaa acggcggacc
      241 ttgaccctgg tccacgcgat gagggcgacg gagacgacag cctgggccgc cagggctatg
      301 atcaggggcg tcatcgatta tcccgatatg tcgacgtggc cgccgagaga tgggtcgctg
      361 atcgtctcgg cctcgtcgtc cccggccggc gtctcctcgc ggcgtcgctc gccgccctgg
      421 ctgccgacgc ccgaacctcc gtcgccattg accgacttgg cggcggcctg gtcgtccttt
      481 ggccgttgta cggccttggc ggcctcgtcc tcgagccgct tcaccacggt gccgtgaagg
      541 ttggcgtgga gcagtgcccc gtccttggcc gccgcctgct ccttgccgac ctgggacaac
      601 tgcaagaaca gggtctgcag gtcaatcggc tttatgggca tccggcggtc ctttccggag
      661 gatctcgtcg tcgaactcct cgtagcgcgt ggtcttgatc atcatgttct cgagatagaa
      721 ggtcaggccc ttgtactcgt tcttgatctt ctcgcggatg tcgcggatca cgatctccga
      781 gccggggtag atcctgcccg agacgctgac cttgccgcga accttcaggt tgttcaggta
      841 ggcctgcagg gcgtcgtact cgtccttgag cgacgaggac tcctgcttga gctcgtcacg
      901 gcggtgggtc agctcgtcca ggacagcctg cttatcgtcc gaaagtgtct tgcgctggcg
      961 tttggtctcg ttcagggtat agatgttctt gtccagctcg tcgatctggc gctggagcag
     1021 cttcatcttg ccgtcaagct cgtccatctt gagcttgctc ttcgggtcgc ttccgacctc
     1081 gagtatcgtc tcggcaccgc ccgacggcga gccgatcgac ttggcattaa tttcctcgca
     1141 ggcgcggtag cggcctccga tgatggcggc gtgcttctgg ccggattggg ctttggcgct
     1201 ctgcgccaga tcggcccg
//
LOCUS       DAOOLN010000818         1217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_153524_length_1217_cov_2.978485, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000818 DAOOLN010000000
VERSION     DAOOLN010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153524_length_1217_cov_2.978485"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="PLW80_09950"
     CDS             complement(<1..936)
                     /locus_tag="PLW80_09950"
                     /inference="COORDINATES: protein motif:HMM:NF010589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HPB66878.1"
                     /translation="MDYIKQAIDYLDRAEAGVIELEKLLTAIPALAPESGGDGEWKKA
                     EALEAWLRARGIVDIERHDAPDARVSAGKRPNLVATIKGRKPGKRLWVMAHTDVVPEG
                     DRSLWDTEPFEAVVKDGKIYGRGVEDNQQGLVSSVFAALALVENGIVPERDVKLLFVA
                     DEEYGSVYGIQWLLENRELFSPDDLIVVPDSGSADGSEIEIAEKNVCWLKLATKGKQC
                     HASRPDDGANAFLANCELALSLNAMERESFPATDPIFVPDRTTITPTKKDANVPNINT
                     VPADDAFYVDMRILPRYSIDEVLAETAKRAAAVERK"
     gene            complement(973..>1217)
                     /locus_tag="PLW80_09955"
     CDS             complement(973..>1217)
                     /locus_tag="PLW80_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPB66879.1"
                     /translation="TNESLVVNLGSEEGLSVLEILEAARRITGKPVPATIEGRRPGDP
                     AKLVASAALAAEKLGWKARYSDVDTLVETSWKAYER"
BASE COUNT          163 a          414 c          400 g          240 t
ORIGIN      
        1 cttccgctcg acggcggccg cccgcttggc tgtctcggcg agcacctcgt cgatcgagta
       61 gcgcggcagg atgcgcatgt ccacgtagaa cgcgtcgtcg gccggcaccg tgttgatgtt
      121 cggcacgttg gcgtccttct tggtcggcgt aatcgtcgtg cggtccggca cgaagatagg
      181 gtcggtcgcg gggaaggatt cgcgctccat cgcgttcagg ctcagggcca gttcgcagtt
      241 cgcgaggaag gcgttggcgc cgtcgtcggg cctggaggcg tggcactgct ttcccttggt
      301 cgccagcttg agccagcaca cgttcttctc ggctatctcg atctcggagc cgtcggccga
      361 gccggagtcc ggtacgacga tcaggtcgtc cggcgagaac agctcgcggt tctcgagcag
      421 ccactggatg ccgtagaccg agccgtactc ctcgtccgcg acgaacagga gcttgacgtc
      481 gcgctcgggg acgataccgt tctccacgag agccagcgcc gcgaacacgc tcgaaaccag
      541 gccctgctgg ttatcctcga cgccgcggcc gtagatcttg ccgtccttca cgacggcctc
      601 gaaaggctcg gtatcccaga gcgagcggtc accctcgggc accacatcgg tgtgggccat
      661 cacccagagg cgtttgcccg gcttcctgcc cttgatggtg gcgaccaggt tcggacgttt
      721 gccggcgctc accctggcgt ccggcgcgtc atggcgctct atgtcgacga tgccgcgggc
      781 gcgcagccag gcctcgaggg cctcggcctt cttccactcg ccgtctccgc cggattccgg
      841 cgccagggcc gggatggccg tcaggagctt ctcgagttcg atgacgcccg cctcggcgcg
      901 gtccaggtag tcgatggcct gcttgatata gtccatgcgt ttcccccttt ttcgttagcc
      961 ggcccctcgg gctcagcgct cgtaggcctt ccagctggtc tcgacgaggg tgtcgacgtc
     1021 ggagtacctg gccttccagc cgagcttctc cgctgcgaga gccgcgctgg cgaccagctt
     1081 ggccggatcg cccggcctgc gcccctcgat ggtcgcgggt accggctttc cggtgatgcg
     1141 cctggccgcc tcgagtatct cgagtaccga caggccctcc tccgagccga ggttgacgac
     1201 gaggctctca ttggtac
//
LOCUS       DAOOLN010000819         1216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_153723_length_1216_cov_2.781223, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000819 DAOOLN010000000
VERSION     DAOOLN010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153723_length_1216_cov_2.781223"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PLW80_09960"
     CDS             complement(<1..500)
                     /locus_tag="PLW80_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HPB66880.1"
                     /translation="MSDNRRVLKETFTTNLSDYAVAIAAVAVVLMIIIPVPTVLLDVL
                     MAFNVALSLMILLLVLSTRRAVDFSVFPTMLLVSTIFGLALNISSTRLILTKGQDFDG
                     RIVRAFSTFVVGAGGTEGLVVGFIIFIVIIAVQAIVITKGATRIAEVAARFTLDGMPV
                     KQMAIEA"
     gene            complement(544..>1216)
                     /locus_tag="PLW80_09965"
     CDS             complement(544..>1216)
                     /locus_tag="PLW80_09965"
                     /inference="COORDINATES: protein motif:HMM:NF013479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HPB66881.1"
                     /translation="EALFNLAKSIFKIVVIGVVAFLNIRGELPRLTRLFSSTVWNSSV
                     YLAGIVIRIVIEAAILMLALAIPDYIFQRAQFKKRLRMTKQEVKEERKMFEGDPMVKG
                     RLKDRMRDIMSRNMAVNVPKADVVVTNPTHFAIAVEFDPMTMAVPTVTAKGADEMAFR
                     IRSLAKDAGVPIVEIRPLARALYAEADVGDAIPEAYYEAISKILAHVAKIDRRVAAKY
                     AAMGR"
BASE COUNT          238 a          372 c          376 g          230 t
ORIGIN      
        1 gcctcgatgg ccatctgctt gaccggcatg ccgtccaggg tgaagcgggc cgccacctcg
       61 gcgatacgcg tcgcgccctt ggtgatgacg atggcctgta cggcaatgat cacgatgaag
      121 atgatgaaac cgacgacgag gccctcggta ccgccggcgc cgacgacgaa ggtgctgaac
      181 gcccgtacga tgcggccgtc gaaatcctgg cccttcgtca ggatcagcct ggtcgacgat
      241 atgttgaggg ccaagccgaa gatggtcgag acgagcaaca tggtcgggaa gaccgagaag
      301 tcgacggccc ggcgagtcga caacacgaga agcaggatca tcagcgacag cgccacgttg
      361 aaggccatca gcacgtcgag gaggaccgtc gggaccggga tgatgatcat cagcacgacg
      421 gccaccgcgg ctatcgccac ggcatagtcc gagaggttgg tggtgaaggt ttccttgagt
      481 actcgcctgt tatccgacat gtcgcctttc catgctatcc taaaaagctc gtttcaacaa
      541 tgatcacctg cccatcgcgg cgtacttggc cgcgaccctc cggtcgatct tggcgacgtg
      601 ggcgagtatc ttcgagatag cctcgtagta ggcctcgggg atcgcgtccc cgacgtcggc
      661 ctcggcgtag agcgcccgcg cgagcggtct aatctccacg atcggtacgc cggcgtcctt
      721 ggccagcgag cgtatgcgga aggccatctc gtcggcgccc ttggccgtga ccgtaggcac
      781 agccatcgtc atcgggtcga actcgacggc tatggcgaag tgagtcgggt tggtcacgac
      841 cacgtcggcc ttcggcacgt tgaccgccat gttcctggac atgatatcgc gcatcctgtc
      901 cttgagcctg cccttcacca tcgggtcgcc ctcgaacatc ttgcgctcct ccttgacctc
      961 ctgcttggtc atcctgaggc gcttcttgaa ctgggcgcgc tggaagatgt agtctggaat
     1021 cgccagggcc agcatcagga tcgcggcctc gatgacgatg cgtatgacga tgcccgccag
     1081 gtagacgctc gaattccata ccgtcgagga gaacaacctc gtcaggcgcg gtagctcgcc
     1141 gcgtatgttg aggaacgcga cgacgcctat cacgacgatc ttgaagatcg acttggccag
     1201 attgaacagc gcctcg
//
LOCUS       DAOOLN010000820         1215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_153942_length_1215_cov_2.313793, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000820 DAOOLN010000000
VERSION     DAOOLN010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153942_length_1215_cov_2.313793"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PLW80_09970"
     CDS             <1..588
                     /locus_tag="PLW80_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66882.1"
                     /translation="LTCLSCDLLGGKAVYLNAQAMEIFHDLIAQSYDIAPKAYVLEDV
                     SETYSGEESDEVKVAGSARLYGDYDYAWDDYYIDANSHKEVRVWAYDGLEFRYAGYKR
                     SSGNVTIVSGSLTIDGTRRMETRYTSTGYTSYSSRTESGSIRVYGTVVVEGVSEGEEV
                     RDELTVDVTRSLSGTSWYYNGTIENRVESWDVYME"
     gene            665..>1215
                     /locus_tag="PLW80_09975"
     CDS             665..>1215
                     /locus_tag="PLW80_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HPB66883.1"
                     /translation="MSRGTGCRYGSHRVLEPKGILPQPALRLDNDMAIYDNEVLIDVS
                     TLNIDSASYTQIRSSCGADEGRMREAILSIVAERGKMQNPVTGSGGMLIGMVREIGPE
                     YPNKQLKVGDRIATLVSLSLTPLRIASIRRLDPDSDQVDVDAQAIMFESGIYANVPTD
                     LPEKLALAVLDVAGAPAQVARIV"
BASE COUNT          257 a          352 c          395 g          211 t
ORIGIN      
        1 ctgacctgcc tttcgtgcga cctgctcggc ggcaaggcgg tctatctcaa cgcccaggct
       61 atggaaatct tccacgacct gatagcgcag agctacgata tagccccgaa ggcgtacgtc
      121 ctcgaggacg tatcggaaac gtactcgggc gaggagtcgg acgaagtcaa ggtggccggc
      181 tcggcaagat tgtacgggga ttatgactac gcctgggacg actactatat cgacgcgaat
      241 tcccataagg aagtacgcgt ctgggcctac gatgggctcg agttccgcta tgcaggctat
      301 aaacgctcga gcgggaacgt cacgatagtc tccggctcgt tgacgattga cggaaccagg
      361 cgcatggaga cccgctacac ctccaccggc tatacctcat actcgagccg gaccgagagc
      421 ggttcgatcc gcgtctacgg cacggtcgtc gtcgagggcg tctccgaggg cgaggaggtt
      481 cgcgacgagc tgacggtcga cgtgacgcgt agcctgagcg gcacaagctg gtactacaac
      541 gggacgatag aaaaccgcgt tgaatcatgg gacgtttaca tggaatgacc ggggtcggcg
      601 ccgacatctt cctgcatcgc gccccgggat gatacgattc cgcccgtcgc gaggaggtag
      661 tgatatgtcc aggggaacgg gatgcaggta cggtagccat agggtgctcg aaccgaaggg
      721 gatactgccg cagccggcgc tcaggctcga taacgatatg gccatctatg acaacgaggt
      781 gctcatcgac gtcagtacgc tcaacataga ctcggctagc tatacgcaga tacgttcgtc
      841 ctgcggggct gatgagggtc gaatgaggga ggccatcctg tccatcgtgg cggagcgggg
      901 caagatgcag aacccggtga ccggctctgg cggcatgctg ataggcatgg tgcgcgagat
      961 agggcccgag tacccgaaca agcagctgaa ggtcggcgac aggatagcca ccctcgtgtc
     1021 gctgtctctg acgccgctca ggatagcctc gatcaggagg ctggacccgg actccgacca
     1081 ggtcgacgtc gacgcccagg ccatcatgtt cgagagcggc atctacgcca acgtgccgac
     1141 ggatctgccc gagaagctgg cgctcgcggt gctcgacgtc gccggcgccc cggcccaggt
     1201 agcccgcatc gtcga
//
LOCUS       DAOOLN010000821         1215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_153961_length_1215_cov_2.173276, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000821 DAOOLN010000000
VERSION     DAOOLN010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153961_length_1215_cov_2.173276"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1215
                     /locus_tag="PLW80_09980"
     CDS             <1..>1215
                     /locus_tag="PLW80_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66884.1"
                     /translation="TASEELAAAYSGGEAAAIDAAWLRARSSAASLAATRRFVVDQGR
                     ALERSFAELSSKDATATDNSFLPFAFRLVLGRQSEGRLEGVAGAIDARWAAALGSAQT
                     ALEERLETEAVAGLAAYDAGEWEAAGDRFSGTAALAERGLALLSLWAHYAPSDLAERS
                     TTLGQAVLELKGEDYLRLRHLAASARAYVALSAVRLAADADERALAAIEPVPENQAAV
                     LTAYESSRLAFVRMQGAIDALRLESGESATRMAEWTRAGYGSDKSQAAQGELDGRIAN
                     ALGLSKTLETGAVARAAAFRFGLLSDEAERALAAIEAARGSLDGVPSDDPQLPEAVFR
                     YPGKALASLSSADRILAVLRADIDAFIASQASRPGYVASDASVLEWTERARTLAATAA
                     ERVADSRALTARA"
BASE COUNT          155 a          475 c          429 g          156 t
ORIGIN      
        1 acagcctccg aggagctcgc cgccgcgtac tcgggcggcg aggccgcggc gatagacgcg
       61 gcttggctcc gggcccgttc gtccgcggcc tccctggccg cgacccgacg cttcgtcgtc
      121 gaccagggac gcgccctcga gcgctccttc gccgaactat cgtccaagga cgcgaccgcc
      181 accgacaact ccttcctccc gttcgccttc cgcctcgtgc tcggccgcca gtccgagggg
      241 cggctcgagg gcgtcgccgg cgcgatcgac gcccggtggg ccgccgctct cggctcggcc
      301 cagaccgctc tcgaggagcg cctcgagacc gaggccgtag cgggcctggc ggcctacgac
      361 gcaggggagt gggaggccgc cggagaccga ttctcgggga cggccgcgct cgccgaacgc
      421 gggctggccc tcctgtcgct ctgggcccac tacgcgccga gcgacctggc cgagcgctcg
      481 acgacgctcg gccaggcggt gctcgagctc aagggtgagg actacctgcg cctgcgccac
      541 ttggcggcga gcgccagggc ctacgtcgcg ctctcggccg tcaggctcgc agccgacgcg
      601 gacgagcgcg cgctcgccgc gatcgagcca gtccccgaga atcaggcggc cgtcctcacg
      661 gcctacgaat ccagccgcct cgcgttcgtc cgcatgcaag gggcgatcga cgcgctcagg
      721 ctcgagtcgg gcgagtcggc gaccaggatg gccgagtgga cgcgagccgg ctacggctcc
      781 gacaagtccc aggccgccca gggcgagctg gacggccgca tagcgaatgc cctcggcctg
      841 tcaaagaccc tcgagaccgg tgcggtggcc cgcgcggccg ccttcagatt cggcctcctg
      901 tccgacgagg ccgagcgcgc tctcgcggcc atcgaggcgg cgcggggctc gctcgacggc
      961 gtgccgtcgg acgaccccca gctgcccgag gccgtgttcc gctaccccgg caaggccctg
     1021 gcctcgctat cgtcggccga ccgcatactc gccgtcctcc gggccgacat cgacgccttt
     1081 atcgcctccc aggcctccag gccgggctac gtggccagcg acgcctccgt gctcgagtgg
     1141 accgagcggg ccaggaccct ggccgcgacg gccgccgagc gcgtggccga ctcgagggcg
     1201 ctgaccgcgc gcgcg
//
LOCUS       DAOOLN010000822         1215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_153997_length_1215_cov_1.863793, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000822 DAOOLN010000000
VERSION     DAOOLN010000822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153997_length_1215_cov_1.863793"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1215)
                     /locus_tag="PLW80_09985"
     CDS             complement(<1..>1215)
                     /locus_tag="PLW80_09985"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HPB66885.1"
                     /translation="YLWLQGALLLWIVSKMLKTVSPRVEMRWFFIVTQYLAVCFIGPP
                     FLSFAVRFRYDRAPSLPLMRALYLVSSAFFGLFATNPLHGLFYSYFDFWRDRFGPIFP
                     WFTGYTYSLIGVSIAFCLMRIRKIRSMAVGLLIATAALIPLVINILYTSRVIRPLFDV
                     TPIAMTTSIFFFALAALRFNFLGPIPVARSIVLETLDDLVVFTDGAGRVVHARGAPRG
                     FVIGSTALSDELTLGDRVYRLHSSRLKGKRGFRCYTDITEIARMQEELARENALLEES
                     LVEITRRNERKLFVLGQDLLQSSRRALHDILGHSLTLVIFLLRRGLSEAARGDAIDER
                     AVLDECAERVREGLAGLDRRLGGQVSQEAVLSLALQGLLAVAPPDAIETELVSAGGER
                     ARPAPDGRNRLK"
BASE COUNT          242 a          355 c          439 g          179 t
ORIGIN      
        1 acttcagccg gttccgcccg tccggggcag gacgcgcccg ctcgccgccg gcgctgacga
       61 gctcggtctc gatggcgtca ggaggggcga ccgccaggag cccttgcagg gccagcgata
      121 gtacggcttc ctgcgatacc tggccgccga gcctgcggtc gaggccggcg agcccctccc
      181 gtacgcgctc ggcgcattcg tcgagaacgg cgcgctcgtc gatcgcgtcg ccccgggccg
      241 cttcgctcag gccgcgcctg agcaggaaga tgaccagcgt cagcgaatgg ccgaggatgt
      301 cgtggagggc gcggcgcgac gactgcagca ggtcctgccc caggacgaag agcttgcgct
      361 cgttgcgccg ggttatctcc actagcgatt cctcgaggag ggcgttctcc ctggcgagct
      421 cctcctgcat acgcgcgatc tccgtgatgt cggtatagca cctgaagccg cgcttcccct
      481 tgagccgcga cgagtggagc cggtagaccc ggtcgcccag cgtcagctcg tcggatagcg
      541 ctgtcgagcc tatgacgaac cctcgcgggg cgccgcgcgc gtggacgact cggccggctc
      601 cgtcggtgaa gacgacgagg tcgtcgaggg tctcgagcac tatcgaccga gctacgggga
      661 tagggccgag gaagttgaag cgcagggccg ccagggcgaa gaagaagata gaggtcgtca
      721 tggcgatggg cgtcacgtcg aagagcgggc gtatgacgcg gctcgtatag aggatgttga
      781 tcacgagggg aatgagggca gccgtcgcta tgagcagccc gacggccatg gagcgtatct
      841 tgcgtatcct cataaggcag aacgcgatgc tgacgcctat gagcgagtac gtatagccgg
      901 tgaaccacgg gaagatcggg ccgaagcggt cacgccagaa atcgaaatag ctatagaaga
      961 gcccatggag gggattggtg gcgaagagcc cgaagaaggc ggaggaaacg aggtataggg
     1021 cgcgcatcag gggtagcgac ggggccctgt catagcggaa gcgcacggcg aaggacagga
     1081 acggcggccc gatgaaacag acggccaggt actgggtgac gatgaagaac cagcgcatct
     1141 ccacgcgggg agagacggtc ttgagcatct tgctgacgat ccacaggagc agggctccct
     1201 ggagccatag atacg
//
LOCUS       DAOOLN010000823         1214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_154094_length_1214_cov_2.969802, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000823 DAOOLN010000000
VERSION     DAOOLN010000823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154094_length_1214_cov_2.969802"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1214
                     /locus_tag="PLW80_09990"
     CDS             <1..>1214
                     /locus_tag="PLW80_09990"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB66886.1"
                     /translation="AMRFETRATAIDRAARTVAVDGPAGEAEIAYDSLILAQGGLPFM
                     PPIEGSDSDNVFRLWTIPDMDAVHSFIEKRGPSSALVVGGGFIGLEAAEAFVARGLAT
                     TVVELTSHVMPPADTEFGAMIGRAFVEAGARVITGRSVKAIRGGVALLDDGTEVPAEL
                     VLVSAGVKPNVELARSAGLELGKTGALVVDEYMRTSDGHIWAAGDMVEIRSRVSGQAT
                     RVPLAGPANRQGRIAATNALGGSMRYAGASGTSVFKAVDKTFAMTGLSERAAKGLGLD
                     AASATVHRYHHVSYYPGAEELSLKLVYERGSGRLLGAQAFGKEGVDKRIDVAATALAG
                     GMTIDDLAELDLAYAPPYGAANDPVNMAAFAAQNAESGYAPTVSAEEAWALAMSGEAS
                     IVDLRTNGDRRK"
BASE COUNT          193 a          422 c          430 g          169 t
ORIGIN      
        1 tggccatgag gttcgagacg agggcgaccg cgatagacag ggcggccagg accgtcgccg
       61 tcgacgggcc ggccggcgag gccgagatcg cctacgactc gctgatactc gcccagggcg
      121 gccttccgtt catgccgccg atagagggct ccgattccga taacgtgttc aggctctgga
      181 ccatcccgga catggacgcc gtgcactcgt tcatcgagaa gcgcgggccc tcgtcggccc
      241 tcgtcgtcgg cggcggcttc ataggcctcg aggccgccga ggccttcgtc gcccgcggac
      301 tggctaccac cgtcgtcgag ctgacctcgc acgtgatgcc gccggccgac accgaattcg
      361 gcgcgatgat aggccgagct ttcgtagagg ccggggccag ggtgatcacc ggacgctcgg
      421 tcaaggcgat acgcggaggc gtagccctgc tcgacgacgg cacggaagtt cccgcggagc
      481 tcgtgctcgt gtccgccggc gtcaagccga acgtcgaact ggccaggtcg gccggcctcg
      541 agctaggcaa gaccggcgcc ctcgtcgtgg acgagtacat gaggacgagc gacggtcata
      601 tctgggccgc cggcgacatg gtcgagatca ggagccgagt ctcggggcag gcgacgcgcg
      661 tgccgctcgc cggcccggcg aataggcagg gccgcatagc cgcgacgaac gcgctcggcg
      721 gctcgatgcg ctacgccggg gcctcgggca cgagcgtctt caaggccgtg gacaagacct
      781 tcgcgatgac cggcctctcc gagcgcgcgg ccaagggcct cggcctggac gccgcctcgg
      841 cgacggtcca tcgctaccac catgtgagct actacccggg cgccgaggag ctgagcctca
      901 agctggtcta cgagcgcggc tcgggccgcc tgctcggggc ccaggccttc ggtaaggagg
      961 gcgtcgacaa gcgcatcgac gtggccgcaa cggcgctcgc cggcggcatg acgattgacg
     1021 acctggccga acttgacctg gcctacgcgc cgccctacgg ggccgctaac gaccccgtca
     1081 acatggccgc cttcgccgcc cagaacgccg agtcgggcta cgcgccgacg gtctcggccg
     1141 aggaggcctg ggcgctcgct atgtcgggcg aggcgtccat cgtcgatctg cggaccaacg
     1201 gcgaccgccg caag
//
LOCUS       DAOOLN010000824         1213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_154297_length_1213_cov_2.878238, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000824 DAOOLN010000000
VERSION     DAOOLN010000824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154297_length_1213_cov_2.878238"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PLW80_09995"
     CDS             complement(<1..816)
                     /locus_tag="PLW80_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66887.1"
                     /translation="MKILCVLLPMQDSSGIGARDNVPLASARAMAWAEETGALSRDEW
                     AFLDGNAADHGGDAAVIASALGSRPELMLFELHAWNLERSLWIARRLRSLLPSTHFAA
                     LGPEVVQGMPVFKAQAFDALIEGEPEAAVVELLADMSARSIKPRYAGSPSVELERLPD
                     PYLSGALPIAPGKTVLIEASRGGATYPAFLRPYPGPRFFARDTAPRVLRLASDGGVDE
                     ARFIDRRLDARPDFKAFVKSLAAVNETGVALSGRLDPAGLDDEAAALLADAAFV"
     gene            complement(850..>1213)
                     /locus_tag="PLW80_10000"
     CDS             complement(850..>1213)
                     /locus_tag="PLW80_10000"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HPB66888.1"
                     /translation="VEAWLDGALVGGLYGLSLGSAFFGESMFSREPDASKAAFIPFVW
                     ALAEAGCTLVDSQVYTDHVASLGGKSVRRDEYLAMLERALASPTIKGDWAAAFPSFPS
                     SRPWLELCEGGVSEVSST"
BASE COUNT          207 a          397 c          445 g          164 t
ORIGIN      
        1 aacgaaggcg gcgtcggcga ggagggccgc ggcctcgtcg tcgagcccgg ccgggtcgag
       61 cctgcccgat agggcgacgc cggtttcgtt gacggcggcc agcgacttga cgaaggcctt
      121 gaagtcgggc cgagcgtcca gcctccggtc gatgaagcgg gcctcgtcga cgccgccatc
      181 ggaggccagc ctgagcacgc ggggagccgt gtcgcgagca aagaacctgg gccccggata
      241 ggggcgtagg aaggccggat acgtggctcc cccgcgggag gcctcgatca gcacggtctt
      301 gccgggagcg ataggcaggg cgccgctcag gtaggggtcg ggcaggcgct cgagctctac
      361 ggacggcgag ccggcatagc gcggcttgat cgacctggcg gacatatcgg cgagtagctc
      421 gacgaccgcc gcctcgggct cgccctcgat caacgcgtcg aaggcctgcg ccttgaagac
      481 gggcatgccc tggaccacct cggggccgag cgccgcgaaa tgcgtcgaag gcaggagcga
      541 gcggagccgc ctggctatcc acagcgaacg ttcgaggttc caggcgtgga gctcgaacaa
      601 catgagttcg gggcgagagc ctagggcgga cgcgatgacc gccgcgtcgc cgccgtggtc
      661 cgcggcgtta ccgtcgagga acgcccattc gtcgcgcgac agcgctccgg tttcctcggc
      721 ccaggccatg gccctggcgg aggccagcgg cacgttgtcg cgggcgccga tgcccgagct
      781 gtcctgcatc ggcaatagta cgcataggat cttcatctcg tggataatcg ctccgttccg
      841 tcgtgacggt caggtagagg atacctcgga aacgccgccc tcgcatagct cgagccacgg
      901 ccgcgacgac gggaaggacg gaaaggcggc ggcccagtcg cccttgatcg tcggggaggc
      961 aagggccctc tcgagcatgg ccaggtattc atcccgccgc actgacttgc cgccgagcga
     1021 cgcgacgtgg tccgtgtaga cctggctgtc gacgagggtg caccccgcct cggcgagcgc
     1081 ccagacgaag ggtatgaagg cagccttgga cgcgtcgggc tcccggctga acatcgactc
     1141 gccgaagaag gccgagccga gcgacaggcc gtacaggccg ccgacgaggg cgccgtcgag
     1201 ccaggcctcg acc
//
LOCUS       DAOOLN010000825         1213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_154309_length_1213_cov_2.732297, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000825 DAOOLN010000000
VERSION     DAOOLN010000825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154309_length_1213_cov_2.732297"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLW80_10005"
     CDS             complement(<1..218)
                     /locus_tag="PLW80_10005"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525450.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPB66889.1"
                     /translation="MSFIQNEDPELYSAIQGEQDRQRKNLELIASENYASKAVMEATG
                     SVLTNKYAEGYPGKRYYGGCEYVDVAENL"
     gene            complement(473..>1213)
                     /locus_tag="PLW80_10010"
     CDS             complement(473..>1213)
                     /locus_tag="PLW80_10010"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPB66890.1"
                     /translation="DDHTGQTSRRIGHDHLVQVYGVDTDVNPDRIKPPPSPAPRLYET
                     LVRPDALVPADDPSIVALAKKAAGREKNHYRIALATLQALNQAVRYDSGAVSESPVKA
                     LEGKVADAWDLAVLYAAALRASGVPALPVAGVVVDDTRRAWNHAWAEFYIYGFGWVPV
                     DPVLASGGDVGRFSPPFEDRSRYFGDMDDRHIAFSRGLAIVDRVTPDGRTVSAARRYS
                     FQNIFEEAAGGLSSYTSFWSDIEITGVY"
BASE COUNT          230 a          377 c          388 g          218 t
ORIGIN      
        1 aggttctcgg ccacgtcgac gtattcgcag ccaccgtagt agcgcttgcc gggatagccc
       61 tcggcgtact tattggtcag caccgagccg gtagcctcca tgacggcctt ggacgcgtag
      121 ttctcgctgg cgatgagctc gagattcttg cgctgcctat cctgctctcc ctgtatggcg
      181 gaatagagtt ccggatcctc gttctggatg aaagacatga gatacctcct tagaagacgc
      241 tacagcatac catactaacc gagcgggtaa aaagcgagcg gtagcgatga atctagccac
      301 agagacacgg aggcacggag aagaggagaa agagcaaagg gcaggattcc gctatacgcg
      361 tcggatgagc ggggcaagga ggcctttagc ggctccccct tatcctaact atccagtcct
      421 atcgcatttc ctccgcgtct ccgtgcctcc gtggttcatt tccccgtcag gcctaatata
      481 cgccggtgat ctctatgtcg ctccagaacg aggtgtacga cgatagcccg cccgcggctt
      541 cctcgaagat gttctggaac gagtagcgcc tcgcggccga gaccgtgcgg ccgtccggcg
      601 tgacgcggtc tacgatggcc aggccccggg agaacgcgat atgcctatcg tccatgtcgc
      661 cgaagtagcg ggatctatcc tcgaacgggg gcgagaaccg gccgacgtct ccgccagacg
      721 cgagcaccgg gtccactggc acccaaccaa agccatagat gtagaactcg gcccaagcgt
      781 ggttccaggc gcgccgggta tcgtccacca cgacgccagc taccggcagg gccggaacgc
      841 ccgaggccct gagcgccgcg gcgtagagca cggccaggtc ccaggcatcg gcgaccttgc
      901 cctcgagcgc cttgaccgga gactcggaca ccgctccgga atcgtagcgg accgcctggt
      961 tgagcgcctg cagggtcgcg agcgcgatgc ggtaatggtt cttctcgcgg ccggctgcct
     1021 tcttggccag ggcgacgatg gagggatcgt cggccggcac gagcgcgtcg ggccggacca
     1081 gcgtctcgta cagcctcggg gccggcgacg gcggcggctt gatgcggtca gggttcacgt
     1141 cggtatccac gccgtagacc tggaccaggt gatcatggcc aattcgacgg gaagtttggc
     1201 cggtgtgatc gtc
//
LOCUS       DAOOLN010000826         1212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_154473_length_1212_cov_3.951599, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000826 DAOOLN010000000
VERSION     DAOOLN010000826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154473_length_1212_cov_3.951599"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PLW80_10015"
     CDS             complement(<1..718)
                     /locus_tag="PLW80_10015"
                     /inference="COORDINATES: protein motif:HMM:NF027610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4932 domain-containing protein"
                     /protein_id="HPB66891.1"
                     /translation="MLKRIPTRRAMARSLSCALAVCACFQAHGHPAVDLPAFSVAVSG
                     DIVFEIDPRLELLSAVQAMTSWAEGPRGPGDRPSRYFRDLTAFIGPYADSRAVRQSQA
                     MTRRGFTYDAPPAFALSLEGGQAMRPPAGGWSDYLRGRAGGKRSLDRFAAALGELYAD
                     SGFDGFLAAHADDYARWLAEARAGFDAERVSAWLEAFYGKPEVPTVYHFVLAPAMFPG
                     GGYGFSRRAGPVGAETLHIYQ"
     gene            complement(723..>1212)
                     /locus_tag="PLW80_10020"
     CDS             complement(723..>1212)
                     /locus_tag="PLW80_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66892.1"
                     /translation="TGNDSATGNNGATEGAGAAGDELAGSSSRRRTRRGPGAEKRLLA
                     PSDAGLAAFDAWLAETTPTSPRVLRLEFVTRLFFATRRDPSAGAALFAAQRAAVIDAI
                     GRLREGLAVSREGLAVSREGLAGDGGAAGFDPSALALSFSEGQLVAVLEWMDASVAPA
                     LS"
BASE COUNT          183 a          426 c          442 g          161 t
ORIGIN      
        1 tctgatagat atgcaacgtc tctgcgccga cgggcccggc ccggcggctg aagccgtagc
       61 cgccaccggg gaacatcgcc ggcgcgagga cgaaatggta gacggtcggg acctccggct
      121 tcccgtagaa cgcctcgagc caggccgaga ctcgttccgc gtcgaagccg gccctcgcct
      181 cggccagcca gcgggcgtag tcgtcggcat gggcggcaag gaagccatcg aagccgctgt
      241 ccgcgtacag ctcgccgagc gccgcggcga agcggtcgag cgagcgcttg ccgccggcgc
      301 gtccgcgcag gtagtcggac cagccgccgg ccggcggccg catcgcctgg ccgccctcga
      361 gcgacagtgc gaaggcgggc ggcgcgtcgt aggtaaagcc tcgcctcgtc atggcctggc
      421 tctggcgtac ggccctgcta tcggcgtacg gcccgatgaa ggccgtgaga tccctgaagt
      481 aacggctagg cctatcgccg gggccgcgcg ggccctccgc ccagctggtc atcgcctgga
      541 cagccgacag gagctcgagc ctcgggtcga tttcgaagac gatatcgcct gagacggcga
      601 cggagaacgc cggaaggtcg acggcaggat gtccgtgggc ctggaagcag gcgcagacag
      661 ccaaggcgca actcagggag cgggccatgg ctcgacgggt cgggattcgt ttgagcatgg
      721 gcctaggata gcgctggggc gacgctcgcg tccatccact cgagcaccgc gacgagttgc
      781 ccctcgctga acgacagcgc aagcgccgac gggtcgaagc cggcggctcc gccatcaccg
      841 gccaggcctt cgcgcgagac ggccaggcct tcacgcgaga cggccaggcc ttcgcgcagg
      901 cgcccgatgg cgtcgatgac ggccgcgcgc tgggcggcga acagcgcggc gccggctgac
      961 ggatctctgc gcgtcgcgaa gaagagccgc gtgacgaact cgaggcgcag tacccgcggg
     1021 ctcgtcggcg tcgtttcagc tagccaggcg tcgaaggcgg ccagccccgc gtcgctcggc
     1081 gccaagaggc gcttctccgc gccgggcccg cgtcgagtcc tacgccggct cgacgagccg
     1141 gccagctcgt cgccggcggc gccggccccc tccgtggcgc cgttgtttcc cgtggcgctg
     1201 tcgtttcccg tg
//
LOCUS       DAOOLN010000827         1211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_154741_length_1211_cov_2.410900, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000827 DAOOLN010000000
VERSION     DAOOLN010000827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154741_length_1211_cov_2.410900"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="PLW80_10025"
     CDS             <1..748
                     /locus_tag="PLW80_10025"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HPB66893.1"
                     /translation="VDSLLAVKFLVFDKKLCSMDELIRALRDNWVGHETLQARAKNQA
                     PKYGRDDDEADAMALRVMELWTGETWKRQTPATGRRYRPGMLSWNYWVGDGYILPASP
                     DGRPKDRFLSNAICPVDGADLNGPTANANSVGKALGGKADGGSGDRDGYVNLLPNGAS
                     HTISFNPSFIRSRENRAKFKAFLRGYAENGGTALQVNMLDPAVLRDAQKRPGDYRHLL
                     VRVTGYNAYFTSIGKELQDEVIARMAHEGL"
     gene            778..>1211
                     /locus_tag="PLW80_10030"
     CDS             778..>1211
                     /locus_tag="PLW80_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPB66894.1"
                     /translation="MGGTTMDTAAVGVEGRVLEIQRMSTEDGPGLRTTLFLKGCSLRC
                     RWCHNPESIDPRPQPRWTAVRCLGCGACVAACPPGALSLGPEGVAIDRSRCDGCGACA
                     AECPATAIELWGVSRDSASLARELLKDEAHFGEDGGVTVSGG"
BASE COUNT          206 a          393 c          452 g          160 t
ORIGIN      
        1 ggtcgactcg ctactcgcgg ttaagttcct ggtcttcgac aagaaactgt gctcgatgga
       61 cgagctgatc cgcgctctca gggacaactg ggtcgggcac gagacgctgc aggcgcgggc
      121 gaagaaccag gcgcccaagt acgggcgcga cgacgacgag gccgacgcga tggccttgcg
      181 ggtgatggaa ctgtggaccg gggagacctg gaagcgccag actccggcga ccgggcggcg
      241 ctatcggccg ggcatgctgt cgtggaacta ctgggtcggc gacggctaca tcctgccggc
      301 cagcccggac ggacggccca aggaccgctt cctgtcgaac gcgatctgcc cggtcgacgg
      361 cgccgacctg aacgggccga ccgcgaacgc gaattccgtg ggcaaggccc tgggaggcaa
      421 ggcggacggc ggctccggcg accgggacgg ctacgtcaac ctgctaccga acggcgccag
      481 ccatacgatc agcttcaacc cgtcgttcat caggagccgc gagaaccgcg ccaagttcaa
      541 ggccttcctg cgcggctacg ccgagaacgg cggcacggcg ctccaggtga acatgctcga
      601 cccggcggtg ctgcgcgacg cccagaagcg accgggcgac taccgccacc tgctggtacg
      661 ggtaacgggc tataacgcat acttcaccag catcggcaag gagctgcagg acgaggtgat
      721 agccaggatg gcccacgagg gcctgtagcc gcggtggtgc tcgcggaggc atggacgatg
      781 gggggaacga cgatggatac ggcggcggtg ggcgtcgagg ggcgggtcct cgagatccag
      841 cggatgagca ccgaggacgg accgggcctc aggacgacgc tattcctcaa gggctgctca
      901 ctacgctgcc gctggtgcca taacccggag agcatcgacc cgcggcccca gcctcgctgg
      961 acggcggtgc gctgcctggg ctgtggcgcc tgcgtcgcgg cctgcccgcc gggagcgctg
     1021 tcgctcgggc cggagggcgt cgccatcgac cggtcgcgtt gcgacggctg cggcgcctgc
     1081 gcggccgagt gcccggcgac cgcgatcgag ctgtggggcg tcagccgcga ctcggcctcg
     1141 ctcgcgaggg agctcctgaa ggacgaggcg cacttcggcg aagacggcgg cgtgaccgtc
     1201 tcgggcggcg a
//
LOCUS       DAOOLN010000828         1210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_154976_length_1210_cov_2.599134, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000828 DAOOLN010000000
VERSION     DAOOLN010000828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154976_length_1210_cov_2.599134"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="PLW80_10035"
     CDS             <1..658
                     /locus_tag="PLW80_10035"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HPB66895.1"
                     /translation="ANDGNAHVGVSWKVRLMGCRFLSAAGGGSTSDAMTCIDYAVAHG
                     AQAINASWGGYGFSQALFDSIVHADLDSAELRSICDYRTYFSYDIRIRDTQSMDPATG
                     KAIDLSLSRVLREKSGGEAQTPYYVAIAASFYRFFKDKPDDSIRFVLFDEAFDKLDDE
                     RMRKVIDFYAQMGIQLMVAVPPGKIEAIAPLSDQICVVSRVGQDARVRDFRSIAGGNA
                     "
BASE COUNT          226 a          419 c          354 g          211 t
ORIGIN      
        1 ggccaacgat ggcaatgccc atgtcggagt ttcctggaaa gtccggttga tgggatgtcg
       61 cttcctctcc gcggccgggg gcggctcgac cagtgacgcc atgacctgca tcgattacgc
      121 tgtcgcgcac ggggcgcaag ccatcaacgc cagttggggc ggatacggtt tctcgcaagc
      181 cctcttcgat agcatcgtgc acgcggacct ggattcggcg gaactgcgga gcatctgcga
      241 ctaccgaacc tatttctcat acgacatccg catccgcgac actcagtcca tggacccggc
      301 caccggcaag gcgatcgacc tatccctctc ccgcgtcctg cgcgagaagt caggcggaga
      361 ggcgcagact ccgtactacg tcgccatagc cgcgagcttc taccgcttct tcaaagacaa
      421 gccggacgac agcatccgct tcgtgctctt cgacgaggcc ttcgacaagc tcgacgacga
      481 gcgcatgcgc aaggtgatcg acttctacgc ccagatgggc atacagctca tggtagccgt
      541 gcccccgggc aagatcgagg ccatagcgcc gctctccgac cagatctgcg tcgtgagccg
      601 cgtcggccag gacgcccgcg tacgcgactt ccgctccatc gccggcggga acgcatgacc
      661 gaatccgcat taagcatctg ccgtgagctc gcgcggcgct accccacctc cgccgccgcc
      721 tcggggggag ccccgctcag gctccccgtc gccagggcct tcccaatcct aacgcgcgct
      781 aagcctgacg cgaaagaatc cttcctggaa gcgtcggaat atttggaagc ggaagggctc
      841 gtcaccctta catggaagcg cttcctcgag ggagatgagc tcgtcagtat aaccctcacg
      901 aacccacgag cactcttcgc ccgtctcggc cgagtctgcc cctcggacga agcggaggcg
      961 gctcgagacg ccgcgcggcg cgtagcccgc gagtcccgcc caaactgccg tgcgttgttc
     1021 tcatggctgg ccgagagcat acggccttcg gatacgatga ccagatccga agggattcgg
     1081 gacgcaaccc ggttgagcga gaccgtaggc gatctagcga ggctgttcga agagatgccc
     1141 tcccataccc gcgacggcat gctctgcggc gtcaccctcc gagcgctctc tatccggctc
     1201 ttctccgact
//
LOCUS       DAOOLN010000829         1208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_155413_length_1208_cov_2.369471, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000829 DAOOLN010000000
VERSION     DAOOLN010000829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155413_length_1208_cov_2.369471"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..>1208
                     /locus_tag="PLW80_10040"
     CDS             198..>1208
                     /locus_tag="PLW80_10040"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPB66896.1"
                     /translation="MRSDKTVDEKSGQWGEASQGDRLALSRTLLSIMKDGVFFADRSL
                     RIRYANHAFVMSTSLDSFVDEPPTLNQLYPEPMNDRPLANAKAALESVGYWSGELTRS
                     RDSSRPSVEEIRLSLAPEASAACSPVDGPCYVGVVRDVTDEREALERLAWTRNHDELT
                     GLPNRTLFAAAIDSVIKRSDAETSSLVVVVADLDDFKRYNTDFGHDCGDLLLRAAGKR
                     VAATVRAGDICARTAGDEFGVLMPVRDPDESHEAAARVQAAFGEPILLDGKSFGLRAS
                     LGVAVWPRDGANATELLAAADVALQACKDAGRNGITQFEEGLYRDRRGRTALETDLRA
                     AIE"
BASE COUNT          224 a          411 c          392 g          181 t
ORIGIN      
        1 tcgcccgcgg gaaaagccgc gaccggagcg acgattacga gcgaggaaag gcagattaat
       61 gccgatacta agtatcgaat caacgccgct cctttcacga caggggtcat ccatgctaga
      121 gtatagcgaa atcttgcctg ccgtgtcgga tacttgcgtg aactcggtcg tggcgaactg
      181 gacggtattc aacgcgtatg cgtagcgata agacggtgga cgagaagagc ggtcagtggg
      241 gcgaggcgtc gcagggcgac aggcttgccc tctcgcgcac cctgctatcc atcatgaagg
      301 acggcgtatt cttcgccgac agaagcctca ggatacggta cgcgaaccac gcgttcgtca
      361 tgtccacgag cctcgactcc ttcgtcgacg agccgccgac tctgaaccag ctttaccccg
      421 agccgatgaa cgaccggccg ctggccaatg ccaaggccgc cctcgagtcg gtaggctact
      481 ggagcggcga gctgactcgc agccgcgatt ccagccgacc gagcgtcgag gaaatacgac
      541 tgagccttgc ccccgaggcg tccgccgcct gttctcccgt ggacggcccc tgctacgtcg
      601 gcgtcgtgag ggacgtcacc gacgagaggg aggcgctcga gcgcctggcc tggacgcgca
      661 accacgacga gctgaccggc ctgccgaacc gtacgctatt cgcggccgcg atcgactcgg
      721 tcatcaagcg cagcgatgcg gagacctcca gcctcgtcgt cgtggtcgcc gacctcgacg
      781 acttcaagcg ctacaacacc gatttcggcc acgactgcgg cgacctgctg ctccgcgccg
      841 ccggcaagcg cgtcgccgcg acggtcaggg caggcgacat ctgcgcgcgg accgccgggg
      901 acgaattcgg cgtcctgatg ccggtgcgcg accctgacga atcgcacgag gcggcggcca
      961 gggtccaggc ggccttcggc gagcccatcc tgctcgacgg caagtcgttc gggttgcggg
     1021 ccagcctcgg cgtcgccgta tggccccggg acggcgccaa cgccaccgag ctgctcgccg
     1081 cggccgatgt agccctccag gcctgcaagg acgccggccg aaacggcatc acccagttcg
     1141 aagagggtct gtaccgcgac cgccgcggcc gtaccgcgct cgagacggac ctacgcgcgg
     1201 ccatcgaa
//
LOCUS       DAOOLN010000830         1207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_155621_length_1207_cov_2.368056, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000830 DAOOLN010000000
VERSION     DAOOLN010000830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155621_length_1207_cov_2.368056"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PLW80_10045"
     CDS             <1..381
                     /locus_tag="PLW80_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66897.1"
                     /translation="FSLLIGVRDRFYVNVFTKKKLRGEAHLVEASYLRRANIDGVDSM
                     DRAEAEAGGKRFVLLWRGADLSGELRAVLESLGQPERLRHFCAYPETKTFFVHLRPKE
                     GDIRDDLSAMLQRMPTFFVEAKEG"
     gene            384..668
                     /locus_tag="PLW80_10050"
     CDS             384..668
                     /locus_tag="PLW80_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66898.1"
                     /translation="MDLPMDVRVKFDAILDGVIEPQSELSLAELGLVKKITYYKADTT
                     IVVYMNYARPTVADCPACSVATDMMKSTIERDLSAALLEAFPGWTVRFDQ"
     gene            complement(714..896)
                     /locus_tag="PLW80_10055"
     CDS             complement(714..896)
                     /locus_tag="PLW80_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HPB66899.1"
                     /translation="MAGKFEWFKDKKDKYRFRLKAGNGQIIATSEAYESKDSCLNGIE
                     SVKKNAPDAPIVETEA"
     gene            998..>1207
                     /locus_tag="PLW80_10060"
     CDS             998..>1207
                     /locus_tag="PLW80_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66900.1"
                     /translation="MPKDCIHFAATALLSCAWLAACSWPRDPSVASEAAIGAYAQALE
                     RYSAGDMAGAAARAARSLGRSGRFLP"
BASE COUNT          208 a          374 c          382 g          243 t
ORIGIN      
        1 ttctccctgc ttatcggggt ccgcgaccgg ttctacgtca atgtcttcac gaagaagaaa
       61 ctgcgcggcg aggcgcacct ggtcgaggcc tcgtacctgc ggcgcgccaa catcgatggc
      121 gtcgactcga tggaccgcgc cgaggcggag gccgggggca agcgcttcgt cctgctgtgg
      181 cgcggcgccg acctgtcagg cgagttgcgg gcggtgctcg agtcgctcgg gcagccggag
      241 cgcttgaggc atttctgcgc ctatcccgaa accaagacct tcttcgtcca cctgcggccg
      301 aaggaagggg acatacggga cgatctttcg gccatgctac agcgcatgcc gacgttcttc
      361 gtagaagcta aggaaggctg accatggatt tgccgatgga cgtcagggtc aagttcgacg
      421 ctatactcga cggggtgatc gagccgcaat ccgagttgtc gctggccgag ctcggcctag
      481 tcaagaagat aacttactat aaggcggata cgaccatcgt ggtctacatg aactacgcca
      541 gacctactgt ggcggactgc ccggcttgca gcgttgcgac cgatatgatg aagtcgacaa
      601 tagagcgcga cctgagcgcg gctctgctcg aggcattccc gggctggacc gtccgcttcg
      661 atcagtagcg ccggcctcga cgagagcggc ggccgaggtg gccgccgctc gcatcatgcc
      721 tccgtctcga cgatcggcgc gtcgggagcg ttcttcttga cgctctctat gccgtttaag
      781 cacgagtcct tcgattcgta ggcctcgctc gtcgcgatga tctggccgtt gcccgccttg
      841 agcctgaaac gatacttgtc ttttttgtcc ttgaaccatt cgaattttcc agccatcgat
      901 acactccttc atgaagatgt gaaattattc taatacgtca tcccttccgg cgcaagcgag
      961 acggccccaa cgggcgtgga tccggcacgg aggagccatg ccaaaggatt gcatccactt
     1021 cgccgccacg gctctgctat cgtgcgcctg gctagccgcc tgttcgtggc ctcgggatcc
     1081 gagcgtggcc agcgaggcgg ccatcggcgc gtacgcccag gcgctcgagc gatattccgc
     1141 tggcgacatg gccggggcgg cggcgcgggc ggcgcggtcg ctcgggcgct ccgggcgctt
     1201 cttaccg
//
LOCUS       DAOOLN010000831         1206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_155770_length_1206_cov_3.135534, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000831 DAOOLN010000000
VERSION     DAOOLN010000831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155770_length_1206_cov_3.135534"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1206
                     /locus_tag="PLW80_10065"
     CDS             <1..>1206
                     /locus_tag="PLW80_10065"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HPB66901.1"
                     /translation="AAYRAALAAVEGDVSLRFRNRLALIRMGRLWTRNVVANLGAVDW
                     PSVGPLRGDGRPIVVCGAGPSLDTAIPALRARRDELFVLACDTASGALALSGVTPDAV
                     VCLEGQIYNLEDFLPLGGRRPRFVADLSAHPSTFRAATGGTSLVLSEYAETRFLSRLS
                     LAGLPLTAVPPLGSVGVLAVRLASALGGPVLVAGLDFAFEPGRTHCAGSPADLRSRRL
                     ESRLCKAESAWNASFRDGVRPIGAGSGEWLTDPALSMYAELAASELRGLEAYDLRCGA
                     GAPLPIRAPLADVAGSAEAVIDAAVRSARDSASAGRRAGTISVAAGPGACRLRAAAFL
                     RGELELAETVAAALRGGSDEQTLRGLILEADFMYCHFPDPERVTALEDDALRRVAAEA
                     AYWRGRLEAA"
BASE COUNT          143 a          439 c          450 g          174 t
ORIGIN      
        1 ggcggcctac cgggcggcgc tcgcggcggt cgagggcgat gtctcgctgc gcttccgcaa
       61 ccgcctggcg ctgatcagga tgggccgact ctggacgagg aacgtcgtcg cgaacctcgg
      121 tgcggtggac tggccctccg tgggcccgct acgcggggac gggaggccga tcgtcgtctg
      181 cggcgccggc ccgagcctcg atacggcgat ccccgccctg agagccaggc gcgacgagct
      241 attcgtactg gcctgcgaca ccgcgtcggg cgcgctcgcc ctgtccggcg tgacgcccga
      301 cgcggtcgtc tgcctcgagg gacagatcta caatctggag gacttcctgc cgctcggcgg
      361 gcggaggccg cgcttcgtgg cggacctgag cgcccacccc tcgaccttcc gcgcggcgac
      421 cggcgggacg agcctcgtcc tgagcgagta cgccgagacc cggttcctgt cgcgcctgtc
      481 cctggccggc ctgccgctca cggccgtgcc gccgctcggc tcggtcggcg tgctggccgt
      541 acggcttgcc tcggcgctcg gcggaccagt cctggtcgcc ggcctggact tcgcgttcga
      601 acccggcagg acacattgcg cgggtagccc ggccgacctc cgctcaagga gactcgagag
      661 ccggctatgc aaggctgaat ccgcctggaa cgcgagcttc cgtgacggcg tcaggccgat
      721 tggcgccgga tccggggaat ggcttaccga ccccgccctg tccatgtacg ccgagctggc
      781 ggcctcggag ctgcgaggtc tcgaagccta cgacctgcgc tgcggcgctg gcgcacccct
      841 gcccatccgc gccccgctgg ctgacgtcgc ggggtcggcg gaggccgtga tcgacgcggc
      901 cgtacgatcc gcgcgcgact ccgcgtcggc ggggcggagg gcggggacga taagcgtggc
      961 ggccggcccc ggcgcctgcc ggctcagggc cgcggccttc ctgcgcggcg agctcgagct
     1021 ggccgagacc gtggccgcgg cgctacgcgg cggctcggac gagcagacgc tccgcgggct
     1081 gatcctcgag gctgatttca tgtattgcca tttcccagac ccggagcgcg tgacggccct
     1141 cgaggacgac gccctcaggc gcgtcgcggc ggaggccgcg tactggcgag gccggctcga
     1201 ggcggc
//
LOCUS       DAOOLN010000832         1205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_155981_length_1205_cov_3.161739, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000832 DAOOLN010000000
VERSION     DAOOLN010000832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155981_length_1205_cov_3.161739"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="PLW80_10070"
     CDS             complement(<1..735)
                     /locus_tag="PLW80_10070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPB66902.1"
                     /translation="MYYERVKQIPLLSAEDERDLSMRIQAGDEAARTQLIEANLRLVI
                     KIARAFSNFDVPLIDLIQEGNMGLIKAAERFDYRRNVRFSTYASWWIKQSVTRSLVNS
                     RRAIRLPHRKEELIRRIHRTYGYLSQALSREPSSLEIANELNIDEKNVQEALEMSGAI
                     MPLEGDSDDSESGCVLDVYADETYSPDAAFARDCLREKTVSLLEALLEKERQVLIYRF
                     ELYGNKRKTLKGIGQSMGISAETVRQI"
     gene            complement(1014..>1205)
                     /locus_tag="PLW80_10075"
     CDS             complement(1014..>1205)
                     /locus_tag="PLW80_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66903.1"
                     /translation="CDSYDERVRSARYGGYMKKLVIGIIVVLAIVTFIRDFSVSPRQV
                     VAQDVGQTMQSSMGTIVTN"
BASE COUNT          256 a          330 c          330 g          289 t
ORIGIN      
        1 gatctgccgc acggtctcag ccgatatacc catgctctgg ccgatgccct tgagcgtctt
       61 gcgcttgtta ccgtacagct cgaagcgata gatcaatacc tggcgttctt tctcgagcag
      121 agcctcgagc aggctgacgg ttttttccct aaggcaatcg cgggcgaagg ccgcgtcggg
      181 gctgtaggtc tcgtcagcgt agacgtccag tacgcagccg ctctccgaat cgtcggagtc
      241 gccctcgagc ggcatgatgg cgcccgacat ctccaaggct tcctgcacgt tcttctcgtc
      301 gatgttgagc tcgttcgcta tctcgaggct gctcggttcc cgtgagaggg cctgggacag
      361 gtatccgtag gtcctgtgga tgcgtcgtat cagctcttcc ttacggtggg gaagccgaat
      421 ggcgcgccgt gagttgacga gcgagcgggt caccgactgc ttgatccacc acgaggcata
      481 ggtggagaaa cgtacgttcc tgcggtagtc gaaccgctcg gccgccttga tcaggcccat
      541 gttcccttcc tggataagat cgataagggg aacgtcgaaa ttcgagaagg cacgcgctat
      601 cttaatgacc agcctgagat tggcttcgat gagttgcgtc ctggcggcct cgtcgccggc
      661 ttggatgcgc atcgagagat cccgctcgtc ctcggcgctg aggagcggta tctgctttac
      721 ccgctcgtag tacattttaa gcgtgtcctc agatattgac ttatttgttc tggcactcat
      781 ttcctgttac ctctgccata tttgatgcaa gaagcgtgcc agaaatttga gagcatgggc
      841 ctctcgtcac taagtaatta tgaaacaacc aaataagctt acaataataa cttgcgatag
      901 catgtcggca gaaacaatag tctacttcag tacacaaaca cgctttttat gcgtctagaa
      961 aataataaac cgccccgtga aggggcggtc taggagtctc gagcgcgtag ccatcagttg
     1021 gtgacaatgg tacccatact cgactgcatg gtctgcccga cgtcttgggc gacaacttga
     1081 cgcggcgaaa cggagaaatc gcgtatgaag gtgacgatgg ccaatacgac tatgattccg
     1141 atgacgagct tcttcatata ccccccgtac ctcgccgatc gtactcgctc gtcgtatgaa
     1201 tcgca
//
LOCUS       DAOOLN010000833         1203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_156407_length_1203_cov_3.134146, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000833 DAOOLN010000000
VERSION     DAOOLN010000833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156407_length_1203_cov_3.134146"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLW80_10080"
     CDS             complement(<1..252)
                     /locus_tag="PLW80_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66904.1"
                     /translation="MSSKEIGATSATQAAPKDEVKFAERLGDFIRANRVGLLVVGVAI
                     LVAIVALGVYSIVSSDRLQKSTVALEALEAGFADWNALEG"
     gene            complement(259..>1203)
                     /locus_tag="PLW80_10085"
     CDS             complement(259..>1203)
                     /locus_tag="PLW80_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPB66905.1"
                     /translation="GYHVLIAKSPDIVEKLDLIDRVAKTDSSVLILGESGVGKELIAE
                     QVHLRSNRKNKPFVRVNCAALPEGLLESELFGHVKGAFTDAVQNRRGRFELADGGTIF
                     LDEIGDLPLKLQAKLLRVLQQKTYERVGSSDTVTVDVRIVAATNRDIDAQVKRGEFRS
                     DLYYRLNVLPVYVPPLRQRKEDIPALAEFFLKKFSRETKKQFAGFTDQAIEHMLSYPW
                     PGNVRELENAVERAVVIAKEKSIGARDLLIGDADTGRDEYRGKSLKDALAIFKKHFIA
                     SALEEHGWNQTETSKVLDIQRTYLSRLIKEFAIANPKE"
BASE COUNT          207 a          363 c          375 g          258 t
ORIGIN      
        1 gccctcaagc gcgttccagt cggcgaagcc ggcctcgagg gcctcgaggg cgacggtgga
       61 cttctggagt ctgtcgctcg agacgatgga gtagaccccg agggcgacga tggcgaccag
      121 tatcgcaacg ccgacgacta acagcccgac gcgattggcg cgtatgaaat caccgagcct
      181 ctcggcgaac tttacctcat ccttgggggc cgcctgagtc gcggacgtgg ccccgatttc
      241 cttagagctc atagatgctt actccttagg gtttgctatg gcgaattctt tgatcaagcg
      301 ggacaggtac gtcctctgaa tgtcgagtac cttcgaggtc tcggtctggt tccagccgtg
      361 ctcctcgagt gcgctcgcta tgaaatgctt cttgaagatg gccagtgcgt ccttgagact
      421 cttgccgcgg tactcgtcgc gtccggtatc ggcgtcgcct atgagcagat ccctcgcgcc
      481 tatcgatttc tccttggcga tgacgacggc tcgctctacg gcattctcga gctcgcggac
      541 gtttcccggc caggggtacg acagcatgtg ctcgatagcc tggtcggtga agccggcaaa
      601 ctgcttcttg gtctcgcggg agaatttctt gaggaagaac tcggccaggg ccggtatgtc
      661 ctccttgcgc tgcctgagag gcggcacgta gacgggcagg acgttcaggc ggtagtacag
      721 gtcgctacgg aactcgccgc gcttaacctg ggcgtcgatg tcccggttgg tggcggcgac
      781 gatgcgcacg tcgaccgtga ccgtatcgct cgagcctacc cgctcgtagg tcttctgctg
      841 aagcacgcgc aggagtttgg cctgcagctt gagcgggagg tcgcctatct catcgaggaa
      901 gatggtgccg ccatcggcca gctcgaagcg gccgcgccgg ttctgcacgg cgtcggtgaa
      961 cgcgcccttg acgtgcccga acagctcgct ctcgagcagt ccctcgggta aagccgcgca
     1021 attgacacgc acgaagggtt tattcttgcg attcgacctc aggtgcacct gctccgcaat
     1081 aagctccttg ccgacgcccg actcgccgag tatcagcacg gacgagtcgg tcttggcgac
     1141 cctatcgatg agatcgagct tctcgacgat atccgggctc ttggctatca gtacgtggta
     1201 gcc
//
LOCUS       DAOOLN010000834         1202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_156606_length_1202_cov_2.993025, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000834 DAOOLN010000000
VERSION     DAOOLN010000834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156606_length_1202_cov_2.993025"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PLW80_10090"
     CDS             complement(<1..704)
                     /locus_tag="PLW80_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66906.1"
                     /translation="MSNLATTRRIAALIIIAVAAGLAASCSAVFRSSIQGSIIDLEDW
                     DDGTTTGVADAKLFLYTDDKARDADYASFVDGDESTLPDGSARTERGYFQSTVTDADG
                     GYDFTGFIWESLFPKYGKTADRDEVFFLIYHPDYGLWKNPTPLYVVSDVTNRLDYIKI
                     EDLWNEGRLAGTVLDWKDDKGLGGVTVNFYVAESWAYDASGGFTDIVYPTAPTATTTA
                     GDDGDWVATVRFPMEP"
     gene            complement(697..>1202)
                     /locus_tag="PLW80_10095"
     CDS             complement(697..>1202)
                     /locus_tag="PLW80_10095"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPB66907.1"
                     /translation="VYSVMVIQFERFMQPLIIMASIPFCLIGVVLGLYLFGSTMSIIA
                     MLAVIALGGTVVNNAIVMVDYINQLRRDYGLELREAILQGCANRLRPILMTALTTLFG
                     VLPMALAHGNGSEVYAPLGQAIFGGLLSSTVITLVIIPVLYESLERGSRFIQSAATAA
                     TELEINE"
BASE COUNT          219 a          360 c          416 g          207 t
ORIGIN      
        1 tccggctcca tcgggaagcg cacggtggcg acccagtcgc cgtcatcgcc ggcggtcgtc
       61 gtcgcggtcg gagccgtggg atagacgatg tccgtgaagc cgccggaggc gtcataggcc
      121 caggactcgg caacgtagaa gttgaccgtc acaccgccga gtcccttgtc gtccttccag
      181 tccagcaccg tccccgcgag ccgcccctcg ttccagaggt cctcgatctt gatgtagtcg
      241 agccggttgg tcacgtcgga gacgacgtag agcggggtcg ggttcttcca gaggccgtaa
      301 tcggggtgat agatcaggaa aaacacctcg tcgcggtcgg cggtcttgcc gtatttcggg
      361 aacagggact cccagatgaa gccggtgaag tcgtagccgc cgtcggcgtc ggtaacggtg
      421 ctctggaagt acccgcgctc ggttcgcgcc gagccgtcgg gcagggtgga ctcgtcgccg
      481 tcgacgaagg aagcgtagtc ggcgtcgcgg gccttgtcgt cggtatagag gaatagcttg
      541 gcgtcggcca cgccggtcgt cgtcccgtcg tcccagtcct ccaggtcgat gatcgaaccc
      601 tggatcgacg agcggaaaac cgccgagcac gacgcggcca ggccggccgc caccgcgatg
      661 atgatgagcg ccgcgatccg gcgcgttgtc gctagattac tcattgatct cgagctccgt
      721 cgcggccgtg gccgccgatt ggatgaagcg ggagccccgt tcgaggctct cgtatagtac
      781 gggtatgatg acgagggtga tgacggtcga agacaggagc ccgccgaaga tggcctggcc
      841 gagcggcgcg tagacctcgg agccgttgcc gtgggccagg gccatcggca gcacgccgaa
      901 cagcgtggtc agggcggtca tcaggatggg cctgagacgg ttggcgcagc cctgcagaat
      961 agcttcccgc agctccaggc cgtagtcccg cctgagctgg ttgatgtagt cgaccatgac
     1021 gatggcgttg ttcacgacgg tgcctccgag cgctatgacc gcgagcatcg cgatgatcga
     1081 catggttgag ccgaacaggt agaggcccag cacgaccccg atcaggcaga acggtatcga
     1141 ggccatgatg atgagcggct gcatgaagcg ctcgaactgg atgaccatga ccgagtagac
     1201 ca
//
LOCUS       DAOOLN010000835         1201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_156780_length_1201_cov_3.420593, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000835 DAOOLN010000000
VERSION     DAOOLN010000835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156780_length_1201_cov_3.420593"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PLW80_10100"
     CDS             <1..199
                     /locus_tag="PLW80_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66908.1"
                     /translation="ALTCCRKALDSDPGNPAYADSIGWTYLRLGMLAEAGRFLGQAAA
                     ELDDEELREHLETYEKAVGSR"
     gene            196..>1201
                     /locus_tag="PLW80_10105"
     CDS             196..>1201
                     /locus_tag="PLW80_10105"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB66909.1"
                     /translation="MRRLVATLTIAVAVSAAGAQFAYEIDAPLAAESFRTGVEAFGRG
                     YYAESLAQLERALASEPRDPLILYWLGKAYLRLGLPSAAFERWGDALSVAGGSPFVES
                     RLELAGAMASPEGLAEPTRYVRVAELSGRKGDTVLFSRPTWIEPLPDGKAIVVSHGTD
                     RLLVVDANGRIVDTLNAGSAGFDRPFACARLDDGTLFVTEFQADRIAHLSPDGRVLGY
                     SGDASGPGRLSGPQYITADADGFLYVSDVGFSRVVKYARDGSMLLSFGARNPTFDGLS
                     LPTGVAVVGDVVYVADAALKAIFSFDRYGNYLGRARVPGLVRPEGLRATGDGRLLVAD
                     G"
BASE COUNT          179 a          435 c          387 g          200 t
ORIGIN      
        1 agccttgacc tgttgccgta aggccctcga ttccgatccc ggcaacccgg cctacgccga
       61 ctcgatagga tggacctatc tgaggctcgg catgctcgcc gaggccggca ggttcctcgg
      121 gcaggccgcg gccgagctcg acgacgagga actacgagag cacctcgaaa cctacgaaaa
      181 ggcggtcgga tcccgatgag aaggctcgtc gcaacgttga ccatcgccgt cgccgtgtcg
      241 gccgcgggcg cccagttcgc ttacgaaata gacgccccgc tcgccgccga gtcgtttcgc
      301 accggcgtcg aggcgttcgg caggggctat tacgccgaat cgctcgccca actcgagcgg
      361 gccctggcga gcgagcctcg cgatccgctg atactctact ggctgggaaa agcctatctc
      421 aggctcggcc tccccagcgc ggcgttcgag cgctggggcg acgcgctgtc cgtcgccggc
      481 ggctcgccct tcgtcgagag caggctcgag ctggccgggg ccatggcctc gcccgagggc
      541 ctggccgaac cgacccgcta cgtccgcgtc gccgagctgt caggcaggaa aggcgacacg
      601 gtgctgttct cgaggccgac ctggatcgag cccttgcccg atggcaaggc catcgtcgtc
      661 tcccacggca ccgaccgctt gctcgtggtc gacgccaatg gccgcatcgt cgataccctg
      721 aacgccggct cggccggttt cgacaggccc ttcgcctgcg ccaggctcga cgacggcacg
      781 ctcttcgtca ccgagtttca ggccgacagg atagcccacc tgtcgcctga cggccgcgtg
      841 ctcggctatt cgggcgacgc ttccgggccg ggtaggctgt ccgggcccca gtacatcacc
      901 gccgacgccg acggcttcct gtacgtcagc gacgtcggct tctcccgcgt cgtcaagtac
      961 gcgcgtgacg gctccatgct gctttctttc ggcgccagga acccgacatt cgacggtctc
     1021 agcctaccga ccggcgtcgc cgtcgtgggc gacgtggtat acgtcgccga cgcggcgctc
     1081 aaggcaatct tttcattcga ccgctacggc aactacctcg gccgcgctcg cgtacccggg
     1141 ctggtgcggc ccgaaggcct ccgggccacc ggcgacgggc gcctgctcgt ggcagacggc
     1201 t
//
LOCUS       DAOOLN010000836         1201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_156854_length_1201_cov_2.390925, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000836 DAOOLN010000000
VERSION     DAOOLN010000836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156854_length_1201_cov_2.390925"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PLW80_10110"
     CDS             complement(<1..560)
                     /locus_tag="PLW80_10110"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HPB66910.1"
                     /translation="MKIDRLLSIVVYLLNRELASASTLAERFGVSTRTIQRDMEAINL
                     AGIPVMSVQGPSGGYGIMDGFKLDRRLVSPDDLFYIVTALNGIAGTLDDGHIAGALET
                     VRGLLPSHGDEGLRRRAERLHVDFSALGGGPAQRDSFRVVQAAVEDGRLLEFEYTSNR
                     LESTRRVVEPMTVVFKWRAWYLFGYC"
     gene            632..>1201
                     /locus_tag="PLW80_10115"
     CDS             632..>1201
                     /locus_tag="PLW80_10115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HPB66911.1"
                     /translation="MDITIRPETKDDYRAVEELTREAFYNLYVPGCDEHYLCHILRGH
                     PDFLPELDYVAEADGRIVGSIMYSKSVLIGDDGENVPIVSFGPVCVHPDYQRKGVGTA
                     LIEATRKLVEAKGVPAIVIYGDPHNYCKHGFRNGVDYRVSDMAGEHPLGLLVLELRPG
                     FFGGTDWKAKQSDAFMFDPAEALAFDEGFP"
BASE COUNT          213 a          426 c          370 g          192 t
ORIGIN      
        1 ctgcagtagc cgaacaggta ccaggcccgc cacttgaaca cgaccgtcat cggctcgacc
       61 acgcgcctcg tcgactccag ccggttgctc gtgtactcga actccaggag ccgcccgtcc
      121 tcgaccgcgg cctgcacgac gcggaacgag tcccgctggg ccggccctcc gcccagcgcg
      181 ctgaagtcca cgtgcagccg ttccgcccgt cgcctgagcc cctcgtcgcc atggctcggg
      241 aggaggccgc gcaccgtctc gagcgcgccc gcgatatggc cgtcgtcgag cgtgcccgcg
      301 attccattga gcgccgtgac gatgtagaac aggtcgtccg gcgagacgag ccgccggtcc
      361 agcttgaacc cgtccatgat gccgtagccg cccgacggcc cctgcacgct catcaccggt
      421 atccccgcca ggttgatcgc ctccatgtcg cgctggatgg tacgcgtcga aaccccgaag
      481 cgttccgcca gggtcgaggc gctcgccagc tcgcggttga ggaggtagac gactatcgat
      541 agcaggcggt cgatcttcat ggggcggctc cgctggggcc cagtatacca tccctcgccg
      601 cttcggagta caatgggccc gaggtacgat catggacatc actatacgcc ccgagaccaa
      661 ggacgattac cgcgccgtcg aggagctgac gcgagaggcg ttctataacc tctacgtgcc
      721 cggctgcgac gagcactacc tctgccatat cctgcgcggg cacccggact tcctgcccga
      781 actcgactac gtggccgagg cggatggacg tatcgtcggc tccatcatgt acagcaagtc
      841 ggtcctgata ggcgacgacg gggagaacgt accgatagtc tccttcggcc cggtatgcgt
      901 ccaccccgac taccagcgca agggcgtcgg cacagccctg atagaggcga cgaggaagct
      961 ggtcgaagcg aaaggcgttc cggccatcgt catctacggc gacccccata actactgcaa
     1021 gcacggcttc aggaacggcg tcgactaccg cgtcagcgac atggccggcg agcaccccct
     1081 cggcctgctc gtcctggagc tcaggccagg cttcttcggc ggcacggact ggaaggccaa
     1141 gcagagcgac gccttcatgt tcgacccggc cgaggcgctc gccttcgacg agggcttccc
     1201 g
//
LOCUS       DAOOLN010000837         1201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_156896_length_1201_cov_2.013962, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000837 DAOOLN010000000
VERSION     DAOOLN010000837.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156896_length_1201_cov_2.013962"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1201
                     /locus_tag="PLW80_10120"
     CDS             <1..>1201
                     /locus_tag="PLW80_10120"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB66912.1"
                     /translation="QQLKETREHYVALSETITEAIFRLDDNFDIIFVNSGVKNTFGWD
                     RDEVIGKPFSVLFPPAVFAQHELEFRKYFYVDDQDRTALGLKRTFEFLGTTKHRGVAP
                     MEMSFGSSKDCRGRTLTCVIRDITQRKTIERKLRHLAYHDKLTGLGNRDLFNEDMAAL
                     LRNQDKDGSWRGAVLFLDLDGFKHVNDTLGHDAGDELLIETARRLRVCLRDDDDAYRF
                     GGDEFVVVLTAIKDASDGVLVSERILASVSSPYILRSKNGTSGGSVSVGVSIGIAVIP
                     EHGHTIEEATKSADIAMYCSKEGGKNRYTLFEPSVSAKATERWRLEQEMKATLQRGGF
                     NLLYQPIVGRDGRILGMEALTRWKRDDERTVMPGEFIPIAEETGMIVALGDWALRRAC
                     YDARRLHE"
BASE COUNT          232 a          393 c          401 g          175 t
ORIGIN      
        1 ccagcagctc aaggagaccc gcgagcacta cgtggcgctg tcggagacca tcaccgaggc
       61 catcttcagg ctcgacgaca acttcgacat catcttcgtc aactccgggg tcaagaacac
      121 cttcggctgg gatcgggacg aggtgatagg caagccgttc agcgtgctat tcccgccggc
      181 ggtgttcgcc cagcacgagc tcgagttccg caagtacttc tacgtcgacg accaggaccg
      241 cacggcgctc gggctcaagc gcaccttcga attcctcggc acgaccaagc accggggcgt
      301 cgcgccgatg gagatgtcgt tcggcagttc caaggactgc cgcggccgga cgctgacctg
      361 cgtgatccgc gacatcacgc agcgcaagac gatagagcgg aagctgcgcc acctggccta
      421 ccacgacaag ctgaccggcc tcgggaaccg cgacctgttc aacgaggaca tggccgcgct
      481 gctgcgcaac caggacaagg acggctcgtg gcggggcgcg gtgctgttcc tcgacctgga
      541 cggcttcaag cacgtgaacg acacgctcgg ccacgacgcc ggcgacgagc tgctcatcga
      601 gacggcaagg cgcctgcgcg tgtgcctgcg cgacgacgac gacgcctacc gcttcggcgg
      661 cgacgaattc gtggtcgtgc tgaccgccat caaggacgcc tcggatggcg tgctggtctc
      721 ggagcgcatc ctcgcgagcg tctcgagccc gtacatactg cgttcgaaga acggcacctc
      781 gggcggctcg gtctccgtcg gagtgtcgat aggcattgcc gtcatccccg agcacggcca
      841 cacgatagag gaggcgacga agagcgccga catagcgatg tactgctcga aggagggcgg
      901 caagaaccgc tataccctgt tcgagccgag cgtcagcgcc aaggcgaccg agcgatggcg
      961 cctcgagcag gagatgaagg cgaccctgca gcgcgggggc ttcaacctgc tgtaccagcc
     1021 catcgtcggg cgggacggca ggatactcgg catggaggcg ctgacccgct ggaagcgcga
     1081 cgacgagcgg acggtgatgc ccggggagtt cataccgata gccgaggaga ccggcatgat
     1141 cgtcgcgctc ggcgactggg ccctcaggcg cgcctgctac gacgcgcgcc gcctgcatga
     1201 g
//
LOCUS       DAOOLN010000838         1199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_157170_length_1199_cov_3.262238, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000838 DAOOLN010000000
VERSION     DAOOLN010000838.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157170_length_1199_cov_3.262238"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PLW80_10125"
     CDS             <1..589
                     /locus_tag="PLW80_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HPB66913.1"
                     /translation="PKNAALTRFEGSYDAETKITTALGIVKNVGVIKSIAVNVKGLNF
                     PHGLSVILKDNDGVEHVMFMGYLNFDGWRELRWDNPQYITDVRNRELRIDPLYPRSTP
                     FVKFAGFLISRDAAAIGGDFIAYVKDVKILYDKAVLEPVRDIDDEAIWGIIGQKEDDR
                     KRLESQRFGASQVMRYLERMKQETKTEFTGSGTSE"
     gene            685..>1199
                     /locus_tag="PLW80_10130"
     CDS             685..>1199
                     /locus_tag="PLW80_10130"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT domain-containing protein"
                     /protein_id="HPB66914.1"
                     /translation="MDASEPIQSRASLERAYKSNAKDIGAILNEMVARIQGALDAVGL
                     FPAYKHRVKSFQSYFAKKIKFTRDAVTNGAPPLPITDLIAVRIICPFIGDLERVEKAL
                     SERFVVREVERKGSERSFKEFGYESTHLLLEIPEDALAGNTFIDPPVCEVQIRTILQD
                     AGRKSSTSWST"
BASE COUNT          259 a          387 c          351 g          202 t
ORIGIN      
        1 tcccaagaac gccgcgctga cccgcttcga gggcagctac gacgccgaga ccaagatcac
       61 caccgccctc ggcatcgtca agaacgtcgg cgtgatcaag tcgatcgccg tcaacgtgaa
      121 gggcctgaat ttcccgcacg gcctgtcggt catcctcaag gataacgacg gcgtcgagca
      181 cgtcatgttc atgggctacc tgaacttcga cggctggcgc gagctgcgct gggacaatcc
      241 ccagtacatc accgacgtac gcaaccgcga gctcaggatc gacccgctct atccgcgctc
      301 gaccccgttc gtcaagttcg ccggcttcct gatctccagg gacgcggccg cgatcggcgg
      361 cgacttcatc gcctacgtca aggacgtaaa gatcctgtac gacaaggccg tcctcgagcc
      421 cgtccgcgac atcgacgacg aggccatctg gggcatcatc ggccagaagg aagacgatcg
      481 caagcggctc gagagccagc gcttcggcgc ctcccaggtc atgcgctatc tcgagcgcat
      541 gaagcaggag acgaagaccg agttcaccgg ttcgggcacc agcgaataac ctcagcgcgc
      601 gcaagcatat acggccaccc gcgacagcgg gtggctattt tttactcgct tgcccgtcca
      661 gccatatcgt cgtatcattg acggatggat gcatccgaac ccatacagag ccgggcgtcg
      721 ctcgagcgag cgtataagtc caacgcgaag gatatcggcg ctatcctgaa cgagatggtc
      781 gccaggatac agggcgccct cgacgccgta ggcttgttcc ccgcctacaa gcacagggtc
      841 aagtcgttcc agagctactt cgccaagaag atcaagttca cgcgcgacgc cgtaacgaac
      901 ggcgcgcccc cgcttccgat aacggacctg atagccgtcc gcatcatctg cccgttcatc
      961 ggcgacctcg agcgcgtaga gaaggcgcta tcggagcgct tcgtcgtgcg cgaggtcgag
     1021 cgcaagggct cggagcggtc cttcaaggaa ttcggctacg agtcgaccca tctgttgctc
     1081 gagatacccg aggacgcgct cgcaggaaat acgttcatag acccgccagt ctgtgaggtg
     1141 cagatacgca cgatacttca ggacgcgggg cggaagtcga gcacgagctg gtctacaag
//
LOCUS       DAOOLN010000839         1199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_157261_length_1199_cov_2.241259, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000839 DAOOLN010000000
VERSION     DAOOLN010000839.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157261_length_1199_cov_2.241259"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..>1199
                     /locus_tag="PLW80_10135"
     CDS             326..>1199
                     /locus_tag="PLW80_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPB66915.1"
                     /translation="MRHSAHTGALVRGAVTAASTLIVALVVGCGAERPDDGTLPVVIV
                     SIEPQAYFVERIAGERVSVSVLVGPGQSPHSYEPTPRQMADLAAASMWLCVGAEFEKT
                     LRPKAAALYKGLPIVDTTKGVEYRRLEAHDHDDDHDDYHDEGGLDPHVWLGRQAVKAM
                     AASIRDALSAADPAGAAAYAANHDAFVRDVDAVYDDLASRLAGLRGTTAFVYHPAFGY
                     LLDEFGIAQEAVETGGKEPTQKALAALIAAARDDGAKLIFVQAQFPTSAAKAVADAIG
                     ASVVPIDPLARDWLD"
BASE COUNT          214 a          407 c          387 g          191 t
ORIGIN      
        1 atccggctat ccgctcgagc gtcgcgtcgc cgacgcttcg tttccgtatc cagcgactct
       61 tggctacggc gtagacgatg gctatcgtcc ccccgatcgc cgcggccagt agcaccgcaa
      121 tgatgttcat cctgatctac ctccagcacg gcgccgtgcg ccgcagaacg cgacgcgagc
      181 agtataaagt caaaaatcgg ccccgacaat gatttcatgc gatgggacca cgaagcttcg
      241 taccaccggg ggcttgccag aacgggcttg agcgagtacc ttttgcgaat cgttcgcact
      301 agtcagtttc gatacggagt atgccatgcg ccattcagca catactggag cgctcgttcg
      361 tggcgcggtc accgcggcct cgacgctgat cgtggcgctc gtggtcggtt gcggggcgga
      421 gcgacccgac gacgggacgc ttcccgtcgt catcgtcagc atcgaaccgc aggcctactt
      481 cgtcgagcgg atagccggag agagggtatc cgtctcggtc ctcgtcggcc ctggccagag
      541 cccgcattca tacgagccga cgcccaggca gatggcggac ctagcggccg cgtcgatgtg
      601 gctatgcgtc ggggccgagt tcgagaagac cctcaggccg aaggccgccg ccctgtacaa
      661 gggactgccc atcgtcgaca cgacgaaggg cgtcgagtat cgccgactcg aggcccatga
      721 ccatgacgac gatcacgacg actaccatga cgaaggcggc ctcgaccccc acgtatggct
      781 cggccgtcag gcggtcaagg cgatggctgc gagcatccgc gacgcgctta gcgccgccga
      841 ccccgcggga gcggccgcct acgccgcgaa ccacgacgcc ttcgtacgcg acgtcgacgc
      901 ggtatacgac gacctcgctt cgaggctcgc ggggctccgg ggcacgaccg ccttcgtcta
      961 ccacccggcc ttcggctacc tgctcgacga gttcggcata gcccaggaag cggtggagac
     1021 cggaggcaag gagccgacgc agaaggcgct cgccgcgttg atcgcggcgg cgcgcgacga
     1081 cggtgccaag ctgatcttcg tgcaggccca gttcccgacg agcgcagcca aggccgtcgc
     1141 cgacgcgata ggcgcgtcgg tggtgccgat cgacccgctc gcgcgcgact ggctcgaca
//
LOCUS       DAOOLN010000840         1196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_157846_length_1196_cov_2.453988, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000840 DAOOLN010000000
VERSION     DAOOLN010000840.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157846_length_1196_cov_2.453988"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="PLW80_10140"
     CDS             complement(<1..796)
                     /locus_tag="PLW80_10140"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB66916.1"
                     /translation="MSTDRTPLVDLALRDDDVLYDKLSIIIDSLDNLHQLDKTAFLKQ
                     VFEAAFLLIPEAQKGSLYELRDGRYRPIFSRGYDEALLSRLTFGESEAFVDFTMDDEG
                     RSSAYETLIIKRNSSAFNPETLELFRRLGTDGGFRTLYAPIRAEGKTFGLISLEKFDD
                     GDFGRFSRKILQYYAWLISEFYAQRLRQEQETGHYREVIGSLISAIEVNDSYTNGHGR
                     RVAAISGRLGMFLGLEPPELRDLHTAALLHDIGKIGVPSEILRKPGG"
BASE COUNT          236 a          318 c          382 g          260 t
ORIGIN      
        1 gcccgcctgg cttgcgcaat atctccgagg gcacgcctat cttgccgatg tcgtggagca
       61 gagcggccgt gtgcaggtcg cgcaactccg gcggctcgag gcccaggaac atgccgaggc
      121 ggccgctgat cgcggcgacg cgcctgccgt ggccattggt gtagctgtcg ttcacctcga
      181 tggcggagat cagcgagcct atcacctcgc ggtagtggcc ggtctcctgc tcctgcctga
      241 ggcgttgagc gtagaattcc gatatcagcc acgcgtagta ctggagtatc ttgcgtgaaa
      301 agcggccgaa atcaccgtcg tcgaatttct ccaggctgat gaggccgaag gtcttgccct
      361 cggcccgtat cggcgcgtac agcgtgcgga agcccccgtc ggtgccgagc cgcctgaaca
      421 gctcgagcgt ttcgggattg aaggccgagg aattgcgctt gatgatcaag gtctcatagg
      481 cgctggatcg gccctcgtcg tccatggtga aatcgacgaa ggcctcgctc tcgccgaacg
      541 tcagcctcga caggagggct tcgtcgtagc cgcgcgagaa gatcgggcgg tacctgccgt
      601 cccgcagctc gtagagggag cccttctgcg cctcgggtat cagcaggaat gcggcctcga
      661 agacctgctt gaggaacgcg gtcttgtcga gctggtgcag gttgtcgagc gagtcgatga
      721 tgatggagag tttgtcgtag agaacatcgt cgtcgcgtag ggctagatcg accagcggcg
      781 tcctgtccgt gctcacgtgg cgcggcctcc tcaggcgatt gctcgagatg gttactgttc
      841 gttgggttac ctagtcgacg taccgcgacc cgctcggagc cttcgaatag tcgaagctat
      901 gtatagcaaa gaacgtgatg tctgtatagc gggcctttgg actctttcga cagaatcgat
      961 atatgcagac tggcttcgcg atggtggata cgatagaacg ggcctgtgtc gtgaaagtgt
     1021 ttcattaaga aacagcaaca tggataaaaa gaaacggatt gtttcgctcc acgccgggtt
     1081 cttcgagaaa gcctcggcgt tgtcatcggc gtatggcgat cgcgtgagct gatatcgagc
     1141 tatgttagta gctaagtata gtcaaaacaa gcaaatagag ccgagcggtg tttatt
//
LOCUS       DAOOLN010000841         1196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_157898_length_1196_cov_2.084137, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000841 DAOOLN010000000
VERSION     DAOOLN010000841.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157898_length_1196_cov_2.084137"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PLW80_10145"
     CDS             complement(<1..681)
                     /locus_tag="PLW80_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66917.1"
                     /translation="MPEIDNRACPDDDTVSLVDLVAVLLKYRRLIIVSTLAAAALAMA
                     AYLIYPPYSLAKAERDRVVEVNASLMLESSLKHYIGEGEGLNYLKQSIGDASNLIVAL
                     RAAGYERIDTIAIGGDVDADKALYTIRRRLIENKSANGTPLKETSRIFSIRDEKGVVS
                     IVFKNGDAEKAKAFMAALIGIVEADVRAFSRPMARATVESFERLLAMPRPSEAVETSI
                     AQGYRDYSA"
     gene            complement(715..>1196)
                     /locus_tag="PLW80_10150"
     CDS             complement(715..>1196)
                     /locus_tag="PLW80_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66918.1"
                     /translation="LGARYQLDEGLNALVSVKRVLWSVDLLVDTVLHYDDKAFYPRVL
                     AGFFREWPDIKLYGEYYYNGETEGGQDHTAGLACGFNNIVGSQLDLGLQWIHAFIDGS
                     GSATAGVTWKPWKYITATVAVPVAYGPDGSRYVTTFNEDVADRRLALVFGLEMEVSF"
BASE COUNT          230 a          366 c          353 g          247 t
ORIGIN      
        1 tgccgaataa tcacgatagc cttgcgctat gctcgtctcc accgcctcgc tcgggcgcgg
       61 catggccagg aggcgttcga aggattccac ggtggcccgg gccatcggcc gcgagaaggc
      121 gcggacgtcc gcttcgacga taccgatgag cgcggccatg aaggccttgg ccttctcggc
      181 gtcgccgttc ttgaacacga tcgagacgac gcccttctcg tctctaatcg agaatatccg
      241 actggtctcc ttgagcggcg tgccgttggc tgacttgttc tcgatcaagc ggcggcgaat
      301 ggtatatagg gccttatcgg cgtctacgtc gccgcctatg gcgatagtat cgatgcgttc
      361 gtagccggcg gcccgcaggg ccacgatcag gttcgacgcg tcgccgatac tctgcttcaa
      421 gtagttcaag ccttcgccct cgccgatgta gtgcttaagg ctcgattcga gcatcaggct
      481 ggcgttgact tccacgacgc gatcccgctc ggccttggcc aggctatagg gcgggtagat
      541 caggtacgcc gccatggcca gcgcagcggc ggccagcgtc gaaacgataa tcaggcgtcg
      601 gtacttgagc agtacggcca ccaggtcgac gagcgatacg gtatcgtcat cgggacaggc
      661 gcgattgtct atctcaggca ttatattact ccctcgggaa cggtgtgctt tctatcagaa
      721 cgagacttcc atctcgagtc cgaataccag agccagtcgg cggtcggcta catcctcgtt
      781 aaaagtcgtt acgtagcgag atccgtccgg gccgtaggct accggtacgg ccacggtcgc
      841 cgttatatac ttccacggct tccaggtaac gcccgcggtc gccgatccgg atccgtcgat
      901 aaaggcgtgg atccactgta ggccgaggtc cagctgcgat cctactatat tattgaagcc
      961 acaggccaga cccgcggtat ggtcctgtcc gccctcggtc tccccgttat agtaatactc
     1021 cccgtacagc ttgatgtccg gccattctcg gaaaaagccc gcgaggaccc tcggatagaa
     1081 ggccttgtcg tcgtagtgaa gcacggtatc taccagaagg tcgacgctcc agagcacccg
     1141 cttgaccgaa acgagagcat tgagcccctc atcgagctgg taacgggcgc cgagcg
//
LOCUS       DAOOLN010000842         1195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_158016_length_1195_cov_3.282456, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000842 DAOOLN010000000
VERSION     DAOOLN010000842.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158016_length_1195_cov_3.282456"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="PLW80_10155"
     CDS             <1..895
                     /locus_tag="PLW80_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPB66919.1"
                     /translation="YGVHALLVIVVSIATAVLSEAAFRAVTKQDVRIGDLSAVVTGLL
                     VALISPPSVPLWMVALGSFAAIVLAKEFFGGLGANPFNPALIGRAILLMSFPAAMTTW
                     HVPFKGLLDATTGATPLNVVKMGGTIADVGAGFVQNSLSSVPDYSSTLWTLFVGNRAG
                     CIGESSILLILVGFIFLLALRVVSFITPLAMVGTAFLASWLLGMDPVFGILSGGLLFG
                     AVFMATDYSSSPVTPLGKAIFGAGAGLITVIIRRFGGYPEGVTYGILIMNAVAPFMDK
                     LRVKKYGFVPPAKPVAKEAAK"
     gene            892..>1195
                     /locus_tag="PLW80_10160"
     CDS             892..>1195
                     /locus_tag="PLW80_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transporter RnfG"
                     /protein_id="HPB66920.1"
                     /translation="MKKDILRLGIALALFAAGACAALAVVYTVTGPTIAGHEREQLQA
                     SLKDLFPDADGFVEIDGSIRSSVPDVTVLGSWKAARGDATLGVAIRASASSYGGDAV"
BASE COUNT          178 a          397 c          366 g          254 t
ORIGIN      
        1 ctacggggtg cacgcgctcc tcgtcatcgt cgtctcgatc gcgaccgccg tgctgtccga
       61 agccgcattc cgcgcggtca cgaagcagga cgtccgtatc ggcgacctgt cggccgtcgt
      121 gaccggtctc ctcgtcgcgc tgatcagccc gccgtccgtc cccctgtgga tggtggccct
      181 cggctcgttc gccgccatcg tactggccaa ggaattcttc ggggggctcg gcgccaatcc
      241 cttcaatccg gcgcttatcg gccgcgcgat tctcttgatg agctttcccg cggccatgac
      301 cacctggcat gtaccgttca agggccttct ggacgctaca accggcgcga cgccgctcaa
      361 cgtcgtcaag atgggtggga cgattgccga cgtcggcgcc ggattcgtac agaattccct
      421 aagctctgtc cccgattaca gctcgacgct gtggacgttg ttcgtgggta accgtgccgg
      481 ctgcatcggc gagagctcga tcctccttat cctggtcggt tttatcttct tgctcgctct
      541 ccgagtggtc tccttcatta ccccgctggc gatggtcgga accgcctttc tagcctcgtg
      601 gcttctaggg atggacccgg tgttcggcat cctatcggga ggactcctat tcggcgcggt
      661 attcatggct accgactact cgtcgagccc ggtgactccg ctcggcaagg cgatattcgg
      721 cgccggcgcg gggctgataa ccgtaatcat ccgccgcttc ggtggctatc ccgaaggcgt
      781 gacctacggc atcctgatta tgaacgccgt ggcgccgttc atggataagc tccgggtcaa
      841 gaaatacgga ttcgtgcctc cggccaagcc cgtcgccaag gaggccgcca aatgaagaag
      901 gacattctca ggttaggcat cgcgttggcg ctcttcgccg ccggcgcgtg cgccgcctta
      961 gccgtcgtct ataccgtaac tggaccgacc atcgcgggcc acgagcgcga gcagctccag
     1021 gcctcgctga aggacctctt tcccgacgcg gatgggttcg tcgagatcga tgggtcgatt
     1081 cgatcctctg tccccgacgt gaccgtcctg ggctcgtgga aggccgcccg gggcgacgcg
     1141 acgctcggcg tggccatccg cgctagcgcc tcgagctacg gtggtgacgc cgtga
//
LOCUS       DAOOLN010000843         1195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_158151_length_1195_cov_1.892982, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000843 DAOOLN010000000
VERSION     DAOOLN010000843.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158151_length_1195_cov_1.892982"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..883)
                     /locus_tag="PLW80_10165"
     CDS             complement(143..883)
                     /locus_tag="PLW80_10165"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPB66921.1"
                     /translation="MMHANEPDDSTRQSEAVSPPDSREPPLRERVYTYLKLKLNDGAL
                     KPGAFLDLNALGVELGLSRTPLRDALLRLEAEGFVTIHPRRGVVLNPLDLQSIRNSYQ
                     LLGAIEAVAIIEVAPTFAPSDVEKMFGLNSQMREALDRNDFDDYYSANLAFHDSYIAR
                     SSNVELKRLARILKDRLYDFPRRESYLSQWERESLKEHEKLATLLMAGSFEEAAAYVR
                     DVHWSFKVQERYIMAYYFAREAALGPML"
     gene            complement(957..>1195)
                     /locus_tag="PLW80_10170"
     CDS             complement(957..>1195)
                     /locus_tag="PLW80_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HPB66922.1"
                     /translation="RVERYGTWSRTIGENIAYGAETARDVVIQLIVDDGVASRGHRAN
                     IFNPGFLVVGIAFGAHPTYRTVCVQDFAGGYTER"
BASE COUNT          241 a          343 c          344 g          267 t
ORIGIN      
        1 tctcgacgct ctcgcgtcgc aatcgcgcaa tcctatcggt catactcgcc tccatgccgt
       61 atcgtcatgt atgcaatata gtacacaagc acattgttcg acctgtccta cagcaaatac
      121 attaacaata ttatacatac tgctaaagca tcgggccgag cgctgcctcc ctggcgaagt
      181 agtaggccat gatatagcgc tcctggacct tgaacgacca atggacgtct cgtacgtagg
      241 ccgcagcctc ttcgaatgat cccgccatca agagcgtagc caacttctcg tgctccttga
      301 ggctctctcg ttcccattgg ctcaggtagc tttcccttcg ggggaaatcg tacaacctat
      361 ccttgagaat gcgggctagg cgcttgagtt caacgttgga gcttctggcg atatacgagt
      421 cgtgaaacgc gagattggcg gagtagtaat cgtcgaagtc gttacggtcg agggcttcgc
      481 gcatctgact gttcaagccg aacatcttct cgacgtctga aggggcgaag gtcggtgcga
      541 cttcgatgat ggcgaccgcc tcgatcgccc cgagcaattg gtatgagttg cgtatcgatt
      601 gcaaatccag aggattgagc acgacgccgc gccttggatg gatggtcacg aagccctcgg
      661 cctcgagcct aagcaaggcg tcgcgcagag gcgtcctgga tagcccgagc tcgacgccga
      721 gcgcgttgag gtcgaggaag gcaccgggct tgagcgcccc gtcattcagt ttcagcttaa
      781 ggtacgtgta gacccgctcc cgtagcgggg gctccctcga gtcaggcggt gagacggctt
      841 cgctctggcg agtgctatcg tccggttcgt ttgcgtgcat catgctgata ttatcttgat
      901 atatcagtct tcgtcaatct tttaatcttc agaacaccag ggatggagca tcgcccctag
      961 cgttcggtgt aacctccggc gaagtcctgg acgcagaccg tgcggtaggt cggatgggct
     1021 ccgaaggcta tgccgaccac gaggaacccg ggattgaaga tgttcgcccg gtggccgcga
     1081 gaggcgaccc catcgtcgac gatcagctgg atgacgacat ccctggccgt ctcggcgccg
     1141 taggcgatgt tctcgccgat ggtccggctc cacgtaccgt aacgctcgac ccgcg
//
LOCUS       DAOOLN010000844         1194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_158336_length_1194_cov_2.124671, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000844 DAOOLN010000000
VERSION     DAOOLN010000844.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158336_length_1194_cov_2.124671"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1194)
                     /locus_tag="PLW80_10175"
     CDS             complement(<1..>1194)
                     /locus_tag="PLW80_10175"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPB66923.1"
                     /translation="IEKAARALGVDPVEIQRKNLLSEGDVSHYGMRMDDVRSRDTFSR
                     CMDKAKWTELRAGIAAYNAANASYKRGAAAIPICFGISFTKIMLNQGGALVHVYNDGS
                     VSVSTGAVEMGQGVGRKIALVASRALGAPLGSVRVESTRTSTVANTVATAASTGSDIN
                     AMAAQVACLEIKKRLVALAARLLGTVPETVDLRDGYVTVGGKADERLSFAAIVAKAFE
                     NRVDLSAHAFYATPGLHYDMKAEAGTPFYYHVYGCAVVTATLDVIRGTYRFDDAIIVH
                     DIAESIDPLVDLGQVEGAFAQGLGWACLEELKFDDTGRLLADTLSTYKLPDAHFMPRM
                     DVEFYAKPNPKVVANSKAVGEPPLIYGLAGYFAVLDALKAARPDGPSFHDLPMTPEKA
                     MLFLT"
BASE COUNT          187 a          369 c          423 g          215 t
ORIGIN      
        1 cggtcaggaa aagcatggcc ttctccggcg tcatcggcag gtcatggaac gacgggccat
       61 cgggcctggc ggccttgagc gcgtcgagga ccgcgaaata gccggctagg ccgtagatta
      121 gaggcggctc gccgacggcc ttggagttgg ccacgacctt ggggttcggc ttcgcgtaga
      181 attcgacgtc catcctcggc atgaaatgcg cgtcgggcag cttgtaggtg gacagggtgt
      241 ccgccaacag acggcccgta tcgtcgaact tgagctcctc gaggcaggcc cagccgagcc
      301 cttgggcgaa ggcgccttcg acctggccga ggtcgacgag cgggtcgatc gactcggcga
      361 tgtcgtggac gatgatcgcg tcgtcgaagc gataggtacc cctgattaca tcgagggtgg
      421 ccgtgacgac ggcgcagccg tagacgtggt agtagaacgg cgtgcccgcc tcggccttca
      481 tgtcgtagtg gaggccgggc gtcgcgtaga aggcatgggc ggacaggtcg acgcggttct
      541 cgaaggcctt ggcgacgatc gcggcgaagc tcagtcgctc gtcggcctta cccccgacgg
      601 tcacgtagcc gtcgcgtagg tcgacggtct ctggaacggt gccgagtagc ctggcggcca
      661 gcgcgacgag gcgcttcttg atctcgaggc aggctacctg ggcggccatc gcgttgatgt
      721 cggaaccggt gctcgccgcg gtggctaccg tgttggcgac tgtgctcgtc ctggtgctct
      781 cgacgcggac cgaaccgagg ggcgccccca gggcgcggga cgccaccagg gctatcttcc
      841 taccgacgcc ctgtcccatc tccacggcgc cggtcgagac gctgaccgag ccgtcgttgt
      901 acacgtggac gagggcgccg ccctggttga gcatgatctt cgtgaagctg atgccgaagc
      961 atatcggtat cgccgcggcg cctcgcttgt aggacgcgtt ggcggcgtta taggcagcga
     1021 tcccggcgcg tagctccgtc cacttcgcct tatccatgca gcgcgagaag gtgtcgcgcg
     1081 agcgcacgtc gtccatcctc atgccgtagt gggacacgtc gccctcggac agcaagttct
     1141 tgcgctggat ctcgaccggg tcgacgccga gggcgcgggc ggccttctcg atgg
//
LOCUS       DAOOLN010000845         1193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_158448_length_1193_cov_3.183656, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000845 DAOOLN010000000
VERSION     DAOOLN010000845.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158448_length_1193_cov_3.183656"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLW80_10180"
     CDS             complement(<1..184)
                     /locus_tag="PLW80_10180"
                     /inference="COORDINATES: protein motif:HMM:NF019814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPB66924.1"
                     /translation="MLMTSDLRVGTVFKSGKDVVVVQRMLGNKGGRGGVVVKMRVKNL
                     LTGSTSDLGLDSGEKFE"
     gene            complement(296..>1193)
                     /gene="earP"
                     /locus_tag="PLW80_10185"
     CDS             complement(296..>1193)
                     /gene="earP"
                     /locus_tag="PLW80_10185"
                     /inference="COORDINATES: protein motif:HMM:NF021598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HPB66925.1"
                     /translation="GRPDWFEAILFDDGRYLDGAGAAATRTIVNLEYLSAEAYPDEFH
                     RLPSLTRSSSVRKHMFMPGFTPATGGLIQDREFAESLRLAGEPGSRRALRSAISDGLG
                     RAVDPDSFWALLFGYERDYGTIVEDLAARAQASPIVAFAAAGKSQPCVEAAWERAGRP
                     FELVGLPFMRQQDWDRLVAAADFALVRGEDSLSRAALAGIPFLWHAYPQGGDQALKVR
                     GLLERMRPFFGPDDFAPIEAAFVDVNDRMADGPTVRGYERIGALFEPSVRVRAGFSAF
                     SETLRKNGDLAANLMTFLREIV"
BASE COUNT          205 a          399 c          407 g          182 t
ORIGIN      
        1 cctcgaactt ctcgcccgag tcgaggccga ggtcggaggt cgagccggtc agcaggttct
       61 tgacccgcat cttgacgacg acgccgccgc ggccgccctt gttgccgagc atgcgctgga
      121 cgacgacgac gtccttgccg gacttgaaga cggttcccac tcgtagatca gatgtcatca
      181 gcatagtatt tccccgtgat aaaagaattg ctatcggtag gcactgacgc cgatggcgct
      241 ggccgcgccg ccggatagcg ctcctggata gagcatcgct tcgaaccgcc gtattctaca
      301 ctatttcgcg caagaaagtc atcaggttgg cggccaggtc cccgttcttc ctgagggttt
      361 cggagaaggc ggagaaaccc gcgcgtaccc gcacggacgg ctcgaacagc gcgccgatcc
      421 tctcgtagcc gcgcaccgta ggcccgtcgg ccatccggtc gttgacgtcg acgaaggccg
      481 cctcgatggg cgcgaagtcg tccggcccga agaaggggcg cattcgctcg aggagccctc
      541 gtaccttgag cgcctggtcg ccgccctgcg gatacgcgtg ccataggaag ggaatgccgg
      601 cgagggcggc tcgcgacagc gagtcctcac cgcgcacgag ggcgaaatcg gcagccgcga
      661 ccaagcggtc ccagtcctgc tggcgcatga acggcaagcc gaccagctcg aaaggccggc
      721 cggcgcgctc ccaggccgcc tcgacgcagg gctggctctt gccggcggcc gcgaaggcta
      781 cgatgggcga cgcttgggcc cgcgcagcca ggtcctcgac gatggtgccg tagtcccgct
      841 cgtagccgaa cagcagggcc cagaagctat ccgggtccac agcgcggccg aggccgtcgc
      901 ttatggccga ccggagggcc cgcctggagc cgggttcgcc cgccagccgg agggactcgg
      961 cgaactcgcg gtcctggatc agacctccgg tggccggcgt gaagcccggc atgaacatgt
     1021 gcttgcgtac cgacgacgag cgggtcaggg acggcaggcg gtgaaactcg tcaggatagg
     1081 cctcggcgct caggtactcg aggttgacga tggtcctcgt cgccgcggcg ccggccccgt
     1141 cgaggtagcg cccgtcgtcg aacaggatcg cctcgaacca gtccgggcga ccg
//
LOCUS       DAOOLN010000846         1193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_158490_length_1193_cov_2.599297, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000846 DAOOLN010000000
VERSION     DAOOLN010000846.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158490_length_1193_cov_2.599297"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1193
                     /locus_tag="PLW80_10190"
     CDS             <1..>1193
                     /locus_tag="PLW80_10190"
                     /inference="COORDINATES: protein motif:HMM:NF023739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HPB66926.1"
                     /translation="CVVLAGDVFDRAVPSAEAVTLLGEFAASLKAADPNLVVAMIPGN
                     HDSAARLAYLSPVLAMAGVHIAADPGDCVRPVVVERGVERLRLWLLPFLTPGAFGSPA
                     EAASPAPTASTAPTNCQGELFADDSATEPDAVRGDAADVAPAAEPMAEPTLRSQADLF
                     AEAMRRTGAAMAVARAADGGARSADVLVCHAFASGGEASESERVFLGNAELVDASGFS
                     AFDYVALGHLHRPQAAGANGRYPGSLLAYSFSEAGSERGFLSVDVSPGGFSAEFRAVR
                     PLRRMACVSGGFDEVLTDPRYDRYAGDYVEATLSDATAVLNPMDALRRRFPFIMSLRQ
                     AAFERGAVEADERRPGRPAASLADDFAVFHREMTGSEPDAATAALFAELYEEASRRAP
                     PSRRP"
BASE COUNT          147 a          443 c          424 g          179 t
ORIGIN      
        1 ctgcgtggtc ctggccggcg acgtgttcga ccgggccgta ccgtcggccg aggcggtaac
       61 cctgctcggc gaattcgcag cgtcattgaa ggccgccgac cccaacctgg tcgtcgcgat
      121 gatacccggc aatcacgact cggccgccag gctcgcttac ctgtcgccgg tgctcgccat
      181 ggccggcgtc catatagccg ccgaccctgg cgactgcgtc cgcccggtcg tcgtcgagcg
      241 cggcgtcgag cggctgcggc tctggctcct gcccttcctg acgcctggcg ccttcggttc
      301 accggccgag gcggcgtcgc cagcgccgac cgcgtcgacc gcgccgacaa attgccaggg
      361 cgagctgttc gccgacgata gcgcgactga gccggacgcc gtccggggcg acgccgcgga
      421 cgtagcgccg gcggccgagc cgatggccga accgaccctg cgttcccagg ccgacctctt
      481 cgccgaggcg atgcgccgga cgggcgccgc gatggccgtg gcgagggccg ccgacggcgg
      541 cgcccggtcg gccgacgtcc tcgtctgcca cgccttcgcc tcgggaggcg aggcgagcga
      601 gtcggagcgc gtcttcctcg gcaacgccga gctggtcgac gcgtcgggct tctccgcctt
      661 cgactacgtg gcgctcggcc acctgcaccg cccccaggcg gccggggcga acggccgcta
      721 tccgggatcg ctgcttgcct atagtttctc ggaggccggg tcggaacgcg gcttcctgtc
      781 ggtcgacgtc tcgccgggcg gcttctcggc agagttcagg gccgtgcggc cgctcaggcg
      841 catggcttgc gtctccggcg gtttcgacga ggtcctgact gacccgcgct acgaccgcta
      901 cgcgggcgac tacgtcgagg ctaccctgtc ggacgcgacg gcggtactca acccgatgga
      961 cgccctacgc cggcgcttcc cgttcatcat gtcgctccgg caagccgcct tcgaacgggg
     1021 cgccgtcgaa gcggacgagc gaaggccggg ccggcctgcc gcttcgctgg ccgacgactt
     1081 cgccgtgttc caccgcgaga tgaccgggag cgagccggac gccgcgacgg cggcgctctt
     1141 cgccgagcta tacgaggagg ctagccgaag agcgccgccg tcgcggcgtc cgg
//
LOCUS       DAOOLN010000847         1191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_158922_length_1191_cov_2.535211, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000847 DAOOLN010000000
VERSION     DAOOLN010000847.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158922_length_1191_cov_2.535211"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1191)
                     /locus_tag="PLW80_10195"
     CDS             complement(<1..>1191)
                     /locus_tag="PLW80_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB66927.1"
                     /translation="RTILNHVVGFGELLSGDADLTTDPAKGKALADIGKAAAALRQPV
                     LEYVGSVIDGSGPSDALRRQVYERIYDLVSLVQAAKRTSSRGDRFIGDVRKVHEAVNA
                     LAALFDEAGPASGKDADRAPAGGRSLEPAKAGRSGRILVVDDDSFNREILARHLERQG
                     HVVCQASNGREAFTILSEAPFDVVVLDIMMPGMSGFQFLEALREDPRLREASTIVVSA
                     LDDPVSMARCIELGAEDYLMRDFDPILLKARIDSLLEKKEYKRQNDAALRRLVETQER
                     LAAELRDAASYVRSLLPKRLRWRGVSADWEFIPSLSLGGDCFYYHHIDERRLAIYLLD
                     VSGHGIQAALLSATIMNMLRGLALGGVDYGSPSSVLSRLNRSFRIEDQNNMYFTIWYG
                     VYDAS"
BASE COUNT          196 a          372 c          415 g          208 t
ORIGIN      
        1 cgaggcgtcg tagaccccgt accagatcgt aaagtacata ttgttctgat cctcgatccg
       61 gaacgagcgg ttgagccgcg acagcaccga cgagggcgag ccgtagtcga cgccgccgag
      121 agccaggccg cggagcatgt tcatgatcgt ggccgacagg agggcggcct ggatgccgtg
      181 tccggagacg tcgagcaggt agatggccag gcggcgctcg tctatgtggt ggtagtagaa
      241 gcagtctccg ccgaggctga gcgatggtat gaattcccag tccgccgaga cgccccgcca
      301 ccggagccgc ttgggtagca ggctgcggac gtacgaggcg gcgtcgcgta gctcggcggc
      361 caaccgctcc tgcgtctcaa cgagccgcct aagcgcggcg tcgttctggc gcttgtattc
      421 cttcttttcg agcaggctgt cgatgcgcgc cttgaggagt atcgggtcaa agtccctcat
      481 gaggtagtct tcggcgccga gctcgatgca gcgggccatg gataccgggt cgtccagggc
      541 cgagacgacg atagtcgacg cctcgcgcag gcgcgggtct tcccgtagcg cctcgaggaa
      601 ctggaagccg ctcatacccg gcatcatgat atccaggacg acgacgtcga acggcgcctc
      661 cgacagtatc gtgaaggcct cgcggccgtt ggacgcctgg cagaccacgt ggccctgccg
      721 ctcgaggtgc ctggcgagta tctcgcggtt gaacgagtcg tcatcgacga ccaggattcg
      781 gcctgaacgc ccggccttgg cgggttcgag gctcctgccg ccggccggcg ccctatcggc
      841 gtccttgccc gaggccgggc cggcttcgtc gaagagggcg gctagcgcgt tgaccgcctc
      901 gtgtacctta cgtacgtcgc ctatgaagcg gtctcccctc gacgaggtcc gcttggccgc
      961 ctggacaagc gagacgaggt cgtagatacg ctcgtagacc tgcctacgga gcgcgtcgga
     1021 gggcccggag ccgtcgatga cggaaccgac gtattccagg accggctgcc tgagggccgc
     1081 ggcggccttg ccgatgtcgg ctagggcctt gcccttggcc ggatccgtgg tcaggtcggc
     1141 atcgccggat agaagctccc cgaagccgac gacgtggttg aggatggttc g
//
LOCUS       DAOOLN010000848         1190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_159200_length_1190_cov_1.880176, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000848 DAOOLN010000000
VERSION     DAOOLN010000848.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159200_length_1190_cov_1.880176"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..285)
                     /locus_tag="PLW80_10200"
     tRNA            complement(204..285)
                     /locus_tag="PLW80_10200"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(249..251),aa:Leu,seq:cag)
     gene            441..674
                     /locus_tag="PLW80_10205"
     CDS             441..674
                     /locus_tag="PLW80_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HPB66928.1"
                     /translation="MKVRVYVPPFADPRAIDDDGFVELADGATLGALLKRLRIPLRPL
                     AATFCLVNYERAGVGHRLADGDTVSFFSLLPGG"
     gene            694..>1190
                     /locus_tag="PLW80_10210"
     CDS             694..>1190
                     /locus_tag="PLW80_10210"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HPB66929.1"
                     /translation="MRGMMGKVLIVDLTTGTMEEKAIPEETYRDYLGGVGLAVRLLTD
                     MIPVGADPLGPGNALAFMSGLLTGTGSMMTGRWMVCCKSPLTGGWGDANCGGTFSPEI
                     KRSGWDGIIFRGVSPKPVAFVSDENGPRLVDASAWWGMDAIESEDAIKASWSGKKKPA
                     IATIG"
BASE COUNT          239 a          334 c          379 g          238 t
ORIGIN      
        1 ttatatattt tcataatatg gttttattac atttgttagt agatagtgtt agtaaatatc
       61 tgaggatttt tcaagaccca agagtaggtg aataaacaat gcggtattca agctttcaac
      121 gtaaatacat acctataaaa gaaatggatt aaccagaaaa agatgaataa tgataaaaaa
      181 agctccccga agggagcttt ctatgcccag agggggactt gaacccccat gcctctcggc
      241 actagtacct gaaactagcg tgtctgccaa ttccaccatc tgggcgtggt agggagtata
      301 taccctggag ggcggcggag agtcaagggc gggcggcgcg ccgagcggtc gctcgatttt
      361 tcgtttgaca tcgaccggct tggcttatag catgatccag ctggcgtggt tcgatcccgg
      421 atacgccgta cggagggtcc ttgaaggtac gcgtctacgt tccgccgttc gcagacccgc
      481 gcgccatcga cgacgacggt ttcgtcgagc tggctgacgg ggcgacgctc ggcgccttgc
      541 tcaagcgcct gcgcatcccg ctcaggccgc tggccgcgac cttctgcctg gtcaactacg
      601 agcgggccgg cgtcgggcat aggctggcgg acggcgacac cgtttcgttc ttctcgctac
      661 tacccggcgg ttgatactgc catcggaggt tctatgcgcg gcatgatggg caaggtcctt
      721 atcgtcgacc tgacgacggg caccatggag gagaaggcta tccccgagga gacctatcgg
      781 gactacctgg gcggcgtcgg gctcgcggtc aggctgctga ccgacatgat accggtcggg
      841 gccgacccgc tcgggccggg caacgcgctc gccttcatga gcggcctcct gaccggcacc
      901 ggctcgatga tgaccggccg ctggatggtc tgctgcaagt cgccgctgac cggaggctgg
      961 ggcgacgcca actgcggcgg cacgttctcg ccggagatca agcggagcgg ctgggacggg
     1021 atcatcttca ggggcgtctc gcccaagccg gtggccttcg tctccgacga gaacggcccg
     1081 cgcctcgtcg acgcgtcggc gtggtggggc atggacgcga tcgagtcgga ggacgcgatc
     1141 aaggcttcct ggtccggcaa gaagaagccg gccatagcga cgataggcca
//
LOCUS       DAOOLN010000849         1189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_159299_length_1189_cov_3.153439, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000849 DAOOLN010000000
VERSION     DAOOLN010000849.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159299_length_1189_cov_3.153439"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..894)
                     /locus_tag="PLW80_10215"
     CDS             complement(148..894)
                     /locus_tag="PLW80_10215"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPB66930.1"
                     /translation="MSRLARFFVASTTSTMDEARRLSAGRERGVVSAGTQSAGRGRLA
                     GRTWVDEPGASLLATFWLPAADYSGAPPSLVAGLLVLEAVRAWVASAGARLGPEPRLK
                     WPNDLLVGGRKLAGVLCESSGGLVYAGVGVNCGQLAFPPGLRTEPTSLRLETGLAPAP
                     EALLDALAEAIADRSAFASGWKARYEAAMAWRGEPVEFRPGLDGEPILGTLVGVDATG
                     AALIALDDVRRAPAAGSATAYASGELRRRY"
     gene            complement(891..>1189)
                     /locus_tag="PLW80_10220"
     CDS             complement(891..>1189)
                     /locus_tag="PLW80_10220"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPB66931.1"
                     /translation="GTGYSSLYYLNRLPIRWLKIDQSFVRDIRQPDASPSNAIVNTII
                     DMARSLGLGTIAEGCETEEQFGFLSRRGCSQVQGYLFSRPAPAAEFEQLIGRAL"
BASE COUNT          173 a          402 c          443 g          171 t
ORIGIN      
        1 gcgcttcttg tcggcgtcgg ggctactcat gccgaagaga cctagcagcc tctggaagaa
       61 tccagaggac gagtcgccgt catcccggct ggccgggtcg ttgttcgtgg gcatacgcgg
      121 aaaccttact atgtcctggt ttactcgtca ataacgacga cggagctcgc ccgaggcgta
      181 cgcggtcgcc gatcctgcgg ccggggccct ccgtacgtcg tcaagggcga tcagcgcggc
      241 gccggtagcg tcgacgccga cgagcgtccc gaggatcggc tctccgtcca ggccgggcct
      301 gaactcgacc ggctcgccgc gccaggccat cgcggcctcg tagcgggcct tccagccgct
      361 cgcgaaggcg ctcctatccg ctatcgcctc ggcgagcgcg tcgaggagcg cctcgggggc
      421 gggggccagg ccggtctcga gccgcagcga ggtcggctcg gtccggaggc ccggtgggaa
      481 ggctagctgg ccgcagttga cgccgacgcc ggcgtagacg agcccgcccg acgactcgca
      541 gagcaccccg gcgagcttgc ggccgcctac gaggaggtcg ttcggccact tgagcctggg
      601 ttcaggcccg aggcgggcgc cggcggaggc gacccaggcg cggacggcct ccagcacgag
      661 cagcccggcg acgagggacg gcggcgcgcc tgagtagtcg gcggcgggta gccagaacgt
      721 ggccaggagg ctggcgcccg gctcgtcgac ccaggtccgc ccggccagcc ggccgcgacc
      781 ggcgctctgg gtgcccgcgc ttacgacgcc gcgttcgcgc ccggccgata ggcggcgcgc
      841 ctcgtccatc gtgctggtcg tcgacgcgac gaagaagcgc gcgaggcggc tcacagcgcg
      901 cggcctatca gctgctcgaa ttcggccgcc ggggccggcc tcgagaagag gtagccctgg
      961 acctgggaac agccgcgcct cgagaggaag ccgaactgct cctcggtctc gcatccctcc
     1021 gctatggtgc cgaggccgag gctcctggcc atgtcgatga tcgtgttgac gatggcgttc
     1081 gacggcgagg cgtccggctg gcgtatgtcg cggacgaagg actggtcgat cttgagccag
     1141 cgtattggca ggcggttcag gtagtagagc gacgagtagc cggtgccga
//
LOCUS       DAOOLN010000850         1187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_159707_length_1187_cov_3.484982, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000850 DAOOLN010000000
VERSION     DAOOLN010000850.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159707_length_1187_cov_3.484982"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1187
                     /locus_tag="PLW80_10225"
     CDS             <1..>1187
                     /locus_tag="PLW80_10225"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPB66932.1"
                     /translation="GGLDTTVIVPWLKEHYDCGVVAVCGDVGQACDWEGLEKKALSSG
                     ASKLVVNDLKKDFVERTLWSLVKAGALYEGRYLLGTAAARPLIAKALAETAIVEGCSA
                     VAHGCTGKGNDQIRFEMGLKAFAPDMEIIAPWRIWDLGSREEEIAYLEAHGIPTPFKK
                     GDSYSRDDNLWHVSHEGLDLEDPMNEPVLEGMLKLCVPPDKAPDEAEYVELTFEKGIP
                     VALNGERTDGVSLVARLNELGGRHGIGILDMVENRVVGMKSRGVYETPAGAILMEAHQ
                     KLEQLCLDRKTMSFKATVAQRFAELMYEGEWYSPLMDAVNAFVDSTQEAVGGVVKLRL
                     WKGSLSCAGMSSPYSLYDHSLASFATGPLFGHRDATGFINLYSLPVKARSLTRPPTSP
                     SSGS"
BASE COUNT          224 a          371 c          397 g          195 t
ORIGIN      
        1 ggcggactcg ataccaccgt catcgtcccc tggctaaagg aacactacga ctgcggggtc
       61 gtcgccgtat gcggcgacgt aggccaggcc tgcgactggg aagggctcga gaagaaggcc
      121 ctgtcgtccg gcgcgtcgaa gctcgtcgtc aacgacctca agaaggattt cgtcgagcgg
      181 acgctctggt ccctcgtgaa ggcgggggcc ctctacgagg ggcgctacct gctcggcacg
      241 gccgcggcca ggcccctgat cgccaaggcc ctggccgaga cggctatagt ggagggctgc
      301 tcggcggtag cgcacggctg caccggcaag ggcaacgacc agatacgctt cgagatgggc
      361 ctcaaggcct tcgctcccga catggagatc atcgcgccgt ggcggatctg ggacctgggt
      421 agccgcgagg aggagattgc ctatctggag gcgcacggca tcccgacgcc gttcaagaaa
      481 ggcgactcct atagccgcga cgacaatcta tggcacgtga gccacgaggg cctcgacctc
      541 gaggatccga tgaacgagcc ggtcctcgag ggcatgctca agctgtgcgt gccgcccgac
      601 aaggcgccgg acgaggccga gtacgtggaa ctcaccttcg agaagggcat acccgtcgcg
      661 ttgaacggcg aacggacgga cggcgtgtcg ctcgtagcca ggctgaacga gctcggcggc
      721 cggcacggga taggcatact cgacatggtc gagaaccggg tcgtcggtat gaagagccgc
      781 ggcgtgtacg agacgccggc cggggcgatc ctcatggagg cgcaccagaa gctggagcag
      841 ctctgcctcg accgcaagac catgtcgttc aaggctacgg tcgcgcagcg cttcgcggag
      901 ctcatgtacg agggcgagtg gtactcgccg ctgatggatg cggtcaatgc gttcgtcgac
      961 tccacccagg aggcagtcgg cggcgtcgtc aagttgaggc tctggaaagg aagcctgtcc
     1021 tgtgccggta tgagttcacc gtactcgctg tatgaccact ctttggccag cttcgcgacc
     1081 gggccattgt tcggccaccg cgacgctacc ggcttcatca acttatactc cctaccggtc
     1141 aaggccagga gcctgactcg gcctccaacc tcgccttcat caggctc
//
LOCUS       DAOOLN010000851         1183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_160590_length_1183_cov_3.800532, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000851 DAOOLN010000000
VERSION     DAOOLN010000851.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160590_length_1183_cov_3.800532"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="PLW80_10230"
     CDS             complement(<1..1029)
                     /locus_tag="PLW80_10230"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HPB66933.1"
                     /translation="MDASPSDNTVLEANMRALRSRSTALAELVAAAEPYPLEPVVARS
                     GDPTATVAGAWLHSRYDPRAEGDRIAAEAAASGASTLLFLGLGLGYAAKAAVDSGATV
                     CAVESDASLLAASLRLVDLSALIASPRFSIALCPRGRGLTDYLNDASPRTIEVIENKA
                     AMAAFPEAAEAMRSQVARYRKRDDMNAATIKRFGRLWVRNLAVNLRQAAASPGVSAIA
                     GAFSGLPALVLAAGPSLDDLADSLPDLAERCVVVCVDTALRSALRYGVTPDFVVVVDP
                     QYWNARHLDRCRADGAILVSEAAVWPSVLRGESWRPGGRTVLCSSIYPLGAYVESRLG
                     QVQGRLGAG"
BASE COUNT          192 a          416 c          407 g          168 t
ORIGIN      
        1 gccggccccg agccgccctt gtacctggcc gagtcggctc tcgacgtagg ctccgagggg
       61 atagatcgac gagcagagga ccgttcgccc gccgggtcgc cacgactcgc cgcgcaggac
      121 cgacggccag acagcggcct cggacaccag tatcgcgccg tcggcccggc accggtccag
      181 gtggcgggcg ttccagtatt gcgggtcgac gacgacgacg aagtccggcg tcacgccgta
      241 gcgtagcgcc gatcgcaggg ccgtgtcgac gcagacgaca acgcaacgct cggccaggtc
      301 cggtagcgaa tcggctaggt cgtccaacga cggcccggcc gccagtacca gagccggcaa
      361 acccgagaag gcgccggcga tagcggatac gccgggcgaa gccgcggcct gccgcaggtt
      421 gacggccagg tttctcaccc ataacctgcc gaagcgcttg atcgtcgccg cgttcatatc
      481 gtcgcgcttt cgataccgcg cgacttggga tcgcatggcc tcggccgcct ccgggaacgc
      541 ggccatggcg gccttgttct cgattacctc gatggtccgc ggagatgcgt cgttaaggta
      601 gtcggtcagt ccccgccctc gagggcacaa cgcgatcgag aagcggggcg atgctataag
      661 cgccgacagg tcgaccaggc ggagcgaggc ggccagcaag gacgcgtccg attccaccgc
      721 gcataccgtc gcgccggagt cgacggccgc cttggccgcg tagccgagcc ccaggccgag
      781 gaacagcagg gtcgaggcgc cgctcgcggc ggcttcagcc gctatcctat cgccctcggc
      841 gcgtggatcg tagcgagagt gtagccaggc gccggcaacc gtggctgtcg gatcgccgct
      901 ccgggcgaca actggctcga gcgggtacgg ctcggccgcc gcgacgagct ccgccaaggc
      961 cgtcgagcgg ctcctcaggg cgcgcatatt cgcctcgagg acggtgttgt cggacggcga
     1021 cgcgtccatc actccagcct caacctgatg acggctccgg ccgctatagg ctccccgggc
     1081 gccggcgact gctcgcgaac ccatccgtcg ccctctatct cgacgattat atcgtcgcgc
     1141 gcgagcaggc cggtcagcgc gcgcttaggc aggccgcgca ggt
//
LOCUS       DAOOLN010000852         1183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_160625_length_1183_cov_3.016844, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000852 DAOOLN010000000
VERSION     DAOOLN010000852.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160625_length_1183_cov_3.016844"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..1037
                     /locus_tag="PLW80_10235"
     CDS             144..1037
                     /locus_tag="PLW80_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPB66934.1"
                     /translation="MQRIGELAAFGTAICWTVSSMFFERATKRVGVLAVNFYKLVFAF
                     GFLVVAGAASRGMPLPLDATRDAWIFLSLSGLVGFVISDIFLFTAYKTIGSRITLLFV
                     ALSPPMTAALGFLFLGETMRPLAIVGMGLVVVGIVIAVLGRRDQAARTVMSRDDKRGY
                     VFALFSAIGQSVGMILTKRGIGDYDALSATQIRVLVGVVGFAVASVLAERGRNLKAAV
                     RDAPGMKSTFSGAIFGPFLGVVLSLYAVQQTNAGVVSTLMALSPVLIIPPAILVLKQK
                     VRFPEIAGAVIAVAGSAIFFL"
BASE COUNT          160 a          433 c          360 g          230 t
ORIGIN      
        1 gccatggggc ctccttccgg gaaccggcta gccgttcccc cgcctacgct aaccatagga
       61 ccgtccgcct gaattgtcaa atcgcggcgg cccgggcgcc ctggcttccc acgcgcggcc
      121 tccgtcggta tagtcgagcc atcatgcagc gaatcggcga actggcggcc ttcggtacgg
      181 ccatctgctg gaccgtatca tcgatgttct tcgagcgggc gacgaagcgc gtcggcgtcc
      241 tggccgtcaa cttctacaag ctcgtcttcg cgttcggctt cctcgtcgtc gccggcgccg
      301 cgtcgcgggg catgcccctg ccgctcgacg cgacgaggga cgcctggatc ttcctctcgc
      361 tgtcgggcct cgtgggcttc gtcatctccg acatcttcct gttcaccgcc tacaagacca
      421 taggctcgcg gatcacgctg ctgttcgtcg ccctatcgcc gcccatgacc gccgccctgg
      481 gcttcctgtt cctcggcgag acgatgcggc cgctggccat cgtcggcatg ggcctggtcg
      541 tcgtcggcat cgtgatcgcc gtgctcggcc ggcgcgacca ggcggccagg accgtgatga
      601 gccgcgacga caaacggggc tacgtcttcg cgctgttctc ggccataggc cagtcggtcg
      661 gcatgattct cacgaaacgc ggcatcggcg actacgacgc cctgtccgcc acccagatcc
      721 gcgtgctcgt cggcgtcgtc ggcttcgccg tcgcttccgt cctggccgag cgcggccgca
      781 acctcaaggc cgccgtccgc gacgcgccgg gcatgaagtc caccttcagc ggcgccatct
      841 tcgggccctt cctcggcgtc gtgctgtcgc tgtacgcggt gcagcagacc aacgccggcg
      901 tcgtcagtac gctcatggcg ctgtccccgg tcctgatcat cccgccggcc atactcgtgc
      961 tcaagcagaa ggtgcgtttc cccgagatag ccggggccgt catcgccgtg gccggctccg
     1021 ccatcttctt cctctaggct atcgcgcagg cttgcggata gccagaaacc ggcctatcct
     1081 gtacctatgg ctatcacctt ctattccgag ggagccgccc gagaggtcac cggctcgaag
     1141 cacctgctcg acgtggacgg agcgcgctac ctggtcgatt gcg
//
LOCUS       DAOOLN010000853         1181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_161105_length_1181_cov_2.398757, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000853 DAOOLN010000000
VERSION     DAOOLN010000853.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161105_length_1181_cov_2.398757"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PLW80_10240"
     CDS             complement(<1..285)
                     /locus_tag="PLW80_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HPB66935.1"
                     /translation="MSVQNGKEIRAKAEAYKDWTAQNLSSIIKVPAMSNKEKERIMFL
                     KGMCEKAGFDEVRIDGLGNLLARVGSGKKILAIDAHIDTVGIGDRSQWKKD"
     gene            complement(282..>1181)
                     /locus_tag="PLW80_10245"
     CDS             complement(282..>1181)
                     /locus_tag="PLW80_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB66936.1"
                     /translation="FETVGLRGVLCYETTDRNGREGAQAGIRENERFARRVDDERKAG
                     GDPLIEAAIGAHAPFTVDDETLSGLAAACSSTGRGLHIHVAEDKFDAVDSRYRFGKDI
                     CVRLDDAGLLGPKTILGHGLYLTPSEVELVNARDSFIAHNARSNMNNCVGYNDKIGQY
                     RNPVLGTDGIGSDMLEEACFAFFKHHDAGGTRWMDAYLEMLQNGNRLLERYFPGKRFG
                     RVEAGYEADLTFWDYDPPTPLESGNVAGHVAFGMASRMVSGTMVAGRFIVKDRKPVFD
                     AAAIAQRGRDETRRLWQRMEERK"
BASE COUNT          163 a          389 c          382 g          247 t
ORIGIN      
        1 gtccttcttc cactgcgagc gatcgccaat gccgacggtg tcgatgtggg cgtcgatcgc
       61 gaggatcttc ttgcccgagc cgacgcgggc caggaggttg ccgaggccgt cgatgcgtac
      121 ctcgtcgaag ccggccttct cgcacatgcc cttgaggaac atgatgcgct ccttctcctt
      181 gttgctcatc gcgggaacct tgatgatgct cgagaggttc tgcgccgtcc agtccttgta
      241 cgcctcggcc ttggccctga tttccttacc gttctgcacg ctcatttcct ttcctccatt
      301 cgctgccata atcgcctggt ctcgtcgcgt ccgcgctgcg ctatggccgc ggcgtcgaat
      361 accggcttcc tgtccttgac tatgaatcgt ccggccacca tcgtgccgga gaccatccta
      421 gaggccatgc cgaaggccac gtgcccggcc acgttgccgc tctcgagcgg ggtaggcggg
      481 tcgtagtccc agaacgtcag gtcggcttcg tagccggcct cgacgcggcc gaagcgcttg
      541 cccgggaagt atcgctcgag cagcctgttg ccgttctgga gcatctcgag gtaggcgtcc
      601 atccatcgcg tgccgccggc gtcgtggtgc ttgaagaacg cgaagcaggc ctcctcgagc
      661 atgtccgagc ctatgccgtc cgtgccgagc accgggttgc gatactggcc gatcttgtcg
      721 ttgtagccga cgcagttgtt catgttcgac cgggcattgt gcgctatgaa cgagtcccgc
      781 gcgttgacca actcgacctc gctcggcgtc aggtagaggc cgtggccgag tatggtcttg
      841 gggccgagga ggcccgcgtc gtcgaggcgc acgcagatgt ccttgccgaa gcggtagcgc
      901 gagtcgaccg cgtcgaattt atcctcggcc acgtggatgt gcagtccgcg cccggtcgac
      961 gagcaggccg cggccaggcc ggagagcgtc tcgtcgtcca cggtgaacgg cgcgtgggcg
     1021 ccgatggccg cttcgatgag cgggtctcct ccggccttgc gctcgtcgtc gacgcggcgg
     1081 gcgaagcgct cgttctcgcg gatgcccgcc tgagctcctt cgcggccgtt gcggtcggtc
     1141 gtctcgtagc atagcacgcc gcgtagaccg accgtctcga a
//
LOCUS       DAOOLN010000854         1180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_161299_length_1180_cov_2.645333, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000854 DAOOLN010000000
VERSION     DAOOLN010000854.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161299_length_1180_cov_2.645333"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..503
                     /locus_tag="PLW80_10250"
     CDS             108..503
                     /locus_tag="PLW80_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66937.1"
                     /translation="MKNQKTKVLVIVEDGRKPLEAIAKTIKTTLVDKAARISGIATSE
                     AIPEILAADAYAFGVGDSASSSWAEIKRLLTGMNLAGRKAGFFVETKGAAVGLKAAFE
                     PAELSVADRDLVAGGQSDTDAWILALLGV"
     gene            543..1076
                     /locus_tag="PLW80_10255"
     CDS             543..1076
                     /locus_tag="PLW80_10255"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HPB66938.1"
                     /translation="MSAGFNLDDAIRKVPDFPKPGILFYDITSVLAKPDAFRYCVDAM
                     VKLYKGADLDAVAAIESRGFLFAAPLADRLGIPLILVRKKGKLPGKTIAKSYCLEYGT
                     AEIEIHEADIPKGGKVLLIDDLIATGGTLRAAADILEEGGAIPAGVFGVIGLPFLHYE
                     KALEGLEIRTLIEYFGE"
BASE COUNT          237 a          379 c          358 g          206 t
ORIGIN      
        1 agggtgcaat tctaggctta ttcgggatat agcaacctcc cgccgcggcc aggcatgcga
       61 ccgtcatgct tgacagcggc ggcggggcgg cttagtatca aagcactgtg aagaaccaaa
      121 aaaccaaggt gctggtcata gtagaggatg ggcgcaagcc gctcgaggct atagccaaga
      181 cgatcaagac gacgctcgtc gacaaggccg ccaggatctc cggtatcgcc acctcggagg
      241 cgataccgga gatcctggcg gccgacgcgt acgcgttcgg cgtcggagac tccgcctcgt
      301 cgtcttgggc cgagatcaag cgcctcttga ccgggatgaa cctggctggc agaaaggccg
      361 gcttcttcgt cgagaccaag ggagccgccg tcggcctcaa agcagccttc gagcccgccg
      421 agctgtcggt cgccgaccgc gacctggtcg ccggcggtca gtccgacacc gacgcttgga
      481 tactggcgct gctcggcgtc tgaaatcccg ctcttcacga tcaaacgcaa cgcgaggtgc
      541 ttatgtccgc aggtttcaac ctagacgacg ccatcaggaa ggtgccggat tttcccaagc
      601 ccggcatcct gttctacgat atcacgagcg tgctggctaa gccggacgcg ttccgctact
      661 gcgtcgacgc catggtcaag ctctataagg gcgccgacct ggacgccgta gccgccatcg
      721 agtcgcgcgg ctttctcttc gccgcgccgc tcgccgacag gctcggcatc ccgctcatcc
      781 tggtacgtaa aaagggcaag ctacccggca agaccatcgc taagagctac tgcctcgaat
      841 atggcaccgc cgagatagag atccacgagg ccgacatccc caagggcggc aaggtcctgc
      901 ttatcgacga cctgatcgcc accggcggca cgctccgcgc ggccgccgac atcctggagg
      961 agggcggcgc cataccggcc ggcgtcttcg gcgtcatagg tttaccgttc ctgcactatg
     1021 agaaggccct cgagggtctc gagatccgga cgctcatcga gtatttcgga gaataaggag
     1081 ccacgaacat gtccatctcc ctcgttaaga ataacgcctg gcactccagg cgaggccccg
     1141 tcgtgctcgt cgtgatggac ggcgtcggct acggcaagta
//
LOCUS       DAOOLN010000855         1179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_161497_length_1179_cov_3.197509, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000855 DAOOLN010000000
VERSION     DAOOLN010000855.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161497_length_1179_cov_3.197509"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..>1179
                     /locus_tag="PLW80_10260"
     CDS             18..>1179
                     /locus_tag="PLW80_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPB66939.1"
                     /translation="MLNARGADHYGRSRLNFLSSFCALAAGMLRSAKLFKELKERAAE
                     IEALERYQASVFSSMTNLLVTSERDGTLRFYNDEAAEAFGLDEGCIGKPVIGLLAGRL
                     DDSVLAAIGNSLRDGTTLLGIEGIAKGDLAKGLRDDLDFSLNMSPVRGRRSDRHDGVV
                     LTFTDQTRERRLKEQMDVVVEERRAIKDMFSRYLSEDVVHSLMESPEQVKLGGAGKRA
                     TVFFADIRGYTSFSEGKTPEYIIGILNEYFSEAVEVVLRYRGFIDKFIGDCIMAAWGV
                     PMTSEEDDARNAVACAIDIQGLISNPKRTFFRGEADHLRVGIGVHTGYLVAGNLGSAR
                     RMNYSVIGDTVNIAARLEGVAKAGEVIITESTKELLGDRFRLESREAVRVKGK"
BASE COUNT          223 a          390 c          385 g          181 t
ORIGIN      
        1 accgagccgg gcttctcgtg ctcaacgccc ggggcgccga ccactacggc cgctcgcgcc
       61 tcaatttcct gtcgtcgttc tgcgccctcg ccgccggcat gctcaggagc gccaagctgt
      121 tcaaggagct caaggagcga gccgccgaga tcgaggcgct cgagcgctac caggcgagcg
      181 tcttctcgtc gatgaccaac ctcctcgtca cctccgagcg cgatgggaca ctgcgtttct
      241 ataacgacga ggcggccgag gccttcggcc tcgacgaagg ctgcataggc aagccggtga
      301 tcgggctact ggccggcagg ctcgacgaca gcgtgctagc cgccatcggc aacagcctgc
      361 gcgacggcac gacgctactc ggcatcgagg gtatcgccaa aggcgacctg gccaagggcc
      421 tgcgcgacga cctggacttc tcgctcaaca tgtcgccggt acgcggcagg cgctccgacc
      481 gccacgacgg cgtcgtgctg acctttaccg accagacgcg ggagcgccgg ctcaaggagc
      541 agatggacgt cgtcgtcgag gagcgcaggg ccatcaagga catgttcagc cgctacctat
      601 cggaggacgt ggtccacagc ctgatggagt cgcccgagca ggtcaagctc ggcggcgcgg
      661 gcaagcgggc gaccgtgttc ttcgccgaca tacgcggtta tacctcgttc tcggagggca
      721 agacgcccga gtacatcatc gggatactca acgagtactt ctccgaggcg gtcgaggtcg
      781 tgcttcggta ccgcggcttc atcgacaagt tcataggcga ctgcatcatg gcagcctggg
      841 gcgtaccgat gaccagcgaa gaggacgacg ctcggaacgc cgtcgcctgc gccatcgata
      901 tccagggcct catcagcaac cccaagcgaa cgttcttccg cggcgaggcc gaccacctgc
      961 gcgtgggcat cggcgtgcac accggctacc tggtcgccgg caacctcggc tcggcgcgca
     1021 ggatgaacta ctcggtcatc ggcgacacgg tcaacatcgc cgccaggcta gagggagtcg
     1081 ccaaggccgg cgaggtcatc atcaccgaat cgaccaagga gttgctcggc gaccggttca
     1141 ggctggagag ccgagaggcc gtcagggtca agggcaagg
//
LOCUS       DAOOLN010000856         1179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_161586_length_1179_cov_2.159253, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000856 DAOOLN010000000
VERSION     DAOOLN010000856.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161586_length_1179_cov_2.159253"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PLW80_10265"
     CDS             complement(<1..378)
                     /locus_tag="PLW80_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPB66940.1"
                     /translation="MAGNALTDVFRIKELRERILYTLTWLVVFRIGAFLPIPGINAGA
                     LKSYFASVSSQGGITDYLDFFAGGAFKNFSVFMLGVMPYISTQIIMQLLLVVFPSLKK
                     MAEEEGGRKKIALYTRLGAIVVAI"
     gene            complement(414..860)
                     /gene="rplO"
                     /locus_tag="PLW80_10270"
     CDS             complement(414..860)
                     /gene="rplO"
                     /locus_tag="PLW80_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPB66941.1"
                     /translation="MSDFNLHPPVGATHRKKIVGRGQGSGLGGTAGKGNKGQQSRSGG
                     KTYPGFEGGQMPLYRRLAHRGFNNKNFRVDFQIVNLNDIEARFEAGEIVDGVSLAIKG
                     LVRKAEKPVKILGNGSLSKALTFVVDKISASAKAKIESVGGKVETP"
     gene            complement(857..1063)
                     /gene="rpmD"
                     /locus_tag="PLW80_10275"
     CDS             complement(857..1063)
                     /gene="rpmD"
                     /locus_tag="PLW80_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPB66942.1"
                     /translation="MAEMIVIKLKKSLIGRKPEQRATVRSLGLRKISSSVVQEATPSI
                     LGMVDAVKHLVEVRPYAPGQEQKK"
BASE COUNT          216 a          359 c          349 g          255 t
ORIGIN      
        1 tatcgctacc acgatggccc cgaggcgggt gtacaacgct atcttcttgc gcccgccctc
       61 ctcctccgcc atcttcttga ggctggggaa cacgacaagc aagagctgca tgatgatctg
      121 ggtcgagatg tagggcatca cgccgagcat gaagaccgag aagttcttga acgctccgcc
      181 ggcgaagaag tcgaggtaat cggtgatgcc gccctgggag ctcaccgacg cgaaatagct
      241 cttgagcgcg ccggcgttaa tgccggggat cggcaggaag gcgccgatcc tgaacacgac
      301 gagccaggtg agggtataga gaatgcgttc ccgcagttct tttatacgga acacgtccgt
      361 caacgcgttg cctgccataa accgttacct tctttcttgt cgcattcgcc cgatcagggc
      421 gtttctacct tgccgccgac cgattcgatc ttggctttgg ctgaggcgga aatcttatcc
      481 acgacgaagg tcagcgcctt tgacagcgag ccgttgccga gaatctttac cggcttctcg
      541 gccttgcgga cgagaccctt tatggcgagc gagacgccgt cgacgatctc gccggcctcg
      601 aaacgggctt cgatgtcgtt caggttgacg atctggaaat cgaccctgaa gttcttgttg
      661 ttgaagcccc tgtgcgctag ccgcctgtac agcggcatct gcccgccctc gaaacccgga
      721 taggtcttgc cgccagagcg ggactgctgt cccttattgc ccttgccggc ggtacctccg
      781 aggccggagc cctggccgcg gcctacgatc ttcttgcgat gcgtggcgcc gacgggggga
      841 tgtagattga agtcgctcat ttcttctgct cctggcccgg cgcgtagggc ctgacctcga
      901 cgaggtgctt gaccgcgtcg accattccga gtatcgaagg agtcgcctcc tgtaccacgc
      961 tcgagcttat cttgcgcagg ccgagagagc gcacggtcgc gcgctgctcg ggcttgcgcc
     1021 ctataaggct cttcttgagc ttgatcacga tcatttcagc catgattaac cccacatatc
     1081 cttgagcgcc ttgccacggt tatgggcgac gacgcgggca tcaaggatgc cctcgatgcc
     1141 gtcaaaggcg gcccggatca cgttgatggc ggaacgact
//
LOCUS       DAOOLN010000857         1178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_161724_length_1178_cov_2.936776, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000857 DAOOLN010000000
VERSION     DAOOLN010000857.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161724_length_1178_cov_2.936776"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PLW80_10280"
     CDS             complement(<1..248)
                     /locus_tag="PLW80_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66943.1"
                     /translation="MALVVLAGAAPFLSALRESFLSGGAYIGLANYRELFADRGFPMA
                     AAITIGWSLLSACAVMVLAYPIASLALGSRRAYAIIFPL"
     gene            complement(350..>1178)
                     /locus_tag="PLW80_10285"
     CDS             complement(350..>1178)
                     /locus_tag="PLW80_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPB66944.1"
                     /translation="LAELQRLPIYTEGRGAYTTSAGTRYEGVYGGVRLLDLLKTYADI
                     GADDSVTFVAMDGYEMSYPGKTVLDQADGEWLLAFRLDGDWLPKDPGYIRTIKVGPKT
                     PNIDGHLSVRMIKKIVVKQKDYVDFTLSLSGKMRFDLDRGTVQSCVSCHTRTVTFERK
                     GVVAEYTGFPLWLLLGYVDDPEYAPHKQDTSIKTYMDDAADAGYAIKLSAIDGYSIIL
                     DSRDITRNDDIIVAMYRGGEKLPPEESPLVLVWDRAAKKIPEGIKNVKMLASVGATF"
BASE COUNT          188 a          352 c          430 g          208 t
ORIGIN      
        1 agcgggaaga ttatggcgta cgcgcgccgc gagccgagcg ccagcgacgc gatggggtag
       61 gccagaacca tgacggcgca ggcggacagg aggctccagc ctatcgtgat ggctgccgcc
      121 atcgggaagc cgcggtcggc gaacagctcg cggtagttgg ccaggccgat gtacgcgccg
      181 cccgagagaa agctctcccg gagagccgag aggaacgggg cggcccccgc caggacgacg
      241 agggccacga gcggcgcgag gcggccgtgg tcggcgaaca ggcccgggac ggcgagcggg
      301 cccgggacgg aggccgagcg ccggtcccgg gcccgctcgg agggcttcgt cagaacgtcg
      361 ctccgacgct ggcgagcatc ttcacgttct tgatgccctc gggaattttc ttggccgcgc
      421 ggtcccagac gaggaccagc ggcgattcct cggggggcaa cttctcgccg ccgcggtaca
      481 tcgcgacgat gatgtcgtcg ttgcgggtga tgtccctgga gtcgaggata atcgaatatc
      541 cgtcgatggc tgacagcttg atggcgtagc ccgcgtcggc ggcgtcgtcc atgtaggtct
      601 tgatggaggt gtcctgcttg tgcggtgcgt actcggggtc gtcgacgtaa cccaggagca
      661 gccatagcgg gaagccggtg tactcggcta cgacgccctt gcgctcgaag gtgacggtcc
      721 tggtatggca gctgacgcag ctctggacgg tgccgcggtc gaggtcgaat cgcatcttgc
      781 ccgagaggct gagggtgaaa tcgacgtagt ccttctgctt gacgactatc ttcttgatca
      841 tgcggaccga taggtgcccg tcgatgttcg gggtcttcgg gccgaccttg atggtgcgga
      901 tgtagccggg atccttgggc agccagtcgc cgtcgagccg gaacgccagg agccattctc
      961 cgtcggcctg gtcgagaacg gtcttgcccg gatagctcat ctcgtagccg tccatcgcca
     1021 cgaaggtcac ggagtcgtcg gcgcctatgt cggcgtaggt cttgagcagg tcgagcaacc
     1081 tgacgccgcc gtagacgccc tcgtagcggg tgccggcgct ggtggtgtag gctccccggc
     1141 cctccgtgta gatcggcagt ctctgtagct ctgcgagg
//
LOCUS       DAOOLN010000858         1176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_162135_length_1176_cov_3.940232, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000858 DAOOLN010000000
VERSION     DAOOLN010000858.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162135_length_1176_cov_3.940232"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PLW80_10290"
     CDS             <1..547
                     /locus_tag="PLW80_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HPB66945.1"
                     /translation="IAKAKMAEDAGAFAFAMDIDAAGLVVLAMQGKPVAPMPVASLSK
                     IASSVRMPFILKGVMTPAGALKALEAGAQAIVVSNHGGRVLDETPSTIEALPPIVEAL
                     HGRMKVLIDGGFRTGLDVFKALALGADGVLIARPFTWSVYGGGAEGAKLYLEKVKAEL
                     AEAMLMTGASSLEEIDGSRLA"
     gene            complement(585..809)
                     /locus_tag="PLW80_10295"
     CDS             complement(585..809)
                     /locus_tag="PLW80_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66946.1"
                     /translation="MAFRPYLVEFKGGTAVTFYKDGKVRSGTLSTRQDLIQSIEPNGG
                     DPRYRSVDAGQEVEFDHNGFILAPGDDRTL"
     gene            complement(965..>1176)
                     /locus_tag="PLW80_10300"
     CDS             complement(965..>1176)
                     /locus_tag="PLW80_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66947.1"
                     /translation="IRSQGEHMAEKETLVIASKVKAFIKDASELKVSAAVIDVLSDKV
                     RSLCEEAIKNAKADGRKTVQEKDFK"
BASE COUNT          205 a          345 c          380 g          246 t
ORIGIN      
        1 catagccaag gcgaagatgg ccgaggacgc cggcgcgttc gccttcgcga tggacatcga
       61 cgccgccggt ctcgtggtgc ttgcgatgca gggtaagccc gtcgcgccga tgccggtcgc
      121 gtcgctgtcc aagatcgcgt cgtcggtacg gatgccgttc atcctaaagg gcgtgatgac
      181 ccctgctggg gcgctcaagg ccctcgaggc aggcgcccag gccatcgtcg tctcgaacca
      241 cggcgggcgc gtgctcgacg agacgccatc gacgatagag gccttgccgc ccatcgtcga
      301 ggcgcttcac ggacgcatga aagtactgat agacggcggc ttcaggaccg gcctcgacgt
      361 attcaaggcg ctggccctgg gcgcggacgg tgtgctgata gccaggccct ttacctggtc
      421 ggtatacggc gggggcgccg agggcgctaa gctatatctg gagaaggtca aggccgaact
      481 ggcggaggct atgctgatga ccggggcctc aagcctcgag gaaattgatg ggagcaggct
      541 ggcttaagcg tcgggtgccg ggatcttcgg atcccggcgt tccctcagag agttcggtcg
      601 tccccggggg cgagtatgaa accgttatgg tcgaattcga cctcttgccc cgcatccacg
      661 gatcggtatc gagggtctcc accgttcggc tcgatggact ggatcaggtc ttgccttgta
      721 gacagggtgc ctgaacggac cttgccgtcc ttgtagaagg taacggcggt accgcccttg
      781 aattcgacca gatagggcct gaaggccatg ccaaagcgcg acttggtcga taaccaagcc
      841 gtggccagct tctgatcctg ggcgagggtg cctttgcgcg gaatcgcctc atcgtgtttg
      901 atgcgcggct tcctttcgac gagctccggg acgtcccgtt tggacgaccc ggcgccacga
      961 agctctactt gaagtctttt tcctggacgg tcttgcggcc atccgccttg gcgttcttga
     1021 tagcctcttc gcagagggag cggaccttat cggacagcac gtcgataact gccgccgaaa
     1081 ccttgagttc ggaagcgtcc ttgatgaacg ccttaacctt ggaggcgatt actagggtct
     1141 ctttttccgc catatgttct ccttgcgatc gaatgc
//
LOCUS       DAOOLN010000859         1175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_162371_length_1175_cov_3.262500, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000859 DAOOLN010000000
VERSION     DAOOLN010000859.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162371_length_1175_cov_3.262500"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /gene="rimM"
                     /locus_tag="PLW80_10305"
     CDS             complement(<1..339)
                     /gene="rimM"
                     /locus_tag="PLW80_10305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPB66948.1"
                     /translation="MDLLALGRVGKPHGVDGRLRLVSYSGETDHLMRLTEATLRGDGR
                     ELFVRIESVTAHGNGVLMKVVGYDSPERAVALSGMELWAPRDMAAPLDDDQYYYADLV
                     GCALVAGGTEL"
     gene            complement(342..575)
                     /locus_tag="PLW80_10310"
     CDS             complement(342..575)
                     /locus_tag="PLW80_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPB66949.1"
                     /translation="MERDLIEYIAKSLVDDPAAVSVNLVETDEGTVVELRVAQDDIGK
                     VIGKRGRIAKSIRTLLSAAATRGGKDAKLLILD"
     gene            complement(680..931)
                     /gene="rpsP"
                     /locus_tag="PLW80_10315"
     CDS             complement(680..931)
                     /gene="rpsP"
                     /locus_tag="PLW80_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPB66950.1"
                     /translation="MSVKIRLKKFGAKRRPYYRIVVLDSRQPRDGKTIDELGIYHPVE
                     KEGAQLRIDEEKARAWLAKGALPTDTVRSLLNKQNIRLG"
BASE COUNT          193 a          374 c          385 g          223 t
ORIGIN      
        1 gagttccgtc ccgccggcga ccagcgcgca gccgacgaga tcggcatagt agtactggtc
       61 gtcgtcgagc ggggccgcca tgtcgcgggg cgcccatagt tccatccccg acaaagccac
      121 ggcccgctcc ggcgagtcgt agccgactac cttcatgagc acgccgttac cgtgggccgt
      181 tacggactcg atgcgaacga acagttcgcg gccgtcgccg cgcagggtag cctcggtaag
      241 acgcatgagg tgatccgtct cgccggagta ggatacgagg cggaggcggc cgtcgacgcc
      301 atggggcttg ccgacgcggc cgagcgcgag gaggtccacc gtcagtcgag gataaggagc
      361 ttggcgtcct taccgccgcg cgtcgcggcc gcggacagca gggtgcggat agacttggcg
      421 atgcggccgc gctttccgat gaccttgccg atgtcgtcct gcgcgacccg cagctcgacg
      481 acggtaccct cgtcggtctc gacgagattg acggagaccg ccgcggggtc gtccacgaga
      541 gacttggcta tatactcgat gagatctcgt tccattcctt ggccctttcg tccgtcgggt
      601 tcgccaggat caccggctat gaaacccgcc tcgaggcttg gcgccggggc ctcgggcgtc
      661 cttaggacgc ccgtaccctt tagccgagac ggatgttctg cttgttaagg aggctgcgga
      721 ccgtatcggt cgggagagcg cccttggcga gccaagctcg ggccttctcc tcgtcgatgc
      781 ggagctgagc gccttccttc tcgaccgggt ggtagatgcc gagctcgtcg atggtcttgc
      841 catcgcgcgg ctggcggcta tcgagcacca cgatgcgata atacgggcgc ctcttggcgc
      901 cgaatttctt gagcctgatc tttacgctca cgaattcctc ctgaaatatt gccggaacgt
      961 cgatttcggt tattgggacg cggtataaaa ccgcgagccg cgccgccgat cgataggcga
     1021 ccgcgacgcg gaagcgccga tactcgcgcc ttgggtttat accgcataag tcgtggcaga
     1081 gtcaatcgtc gggcggatga ccgcttgcta tcggccaaga cggcggcgta tagtgcgccg
     1141 atgcagccac ttccgccccg ccccctgatg ctcgc
//
LOCUS       DAOOLN010000860         1175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_162378_length_1175_cov_3.116964, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000860 DAOOLN010000000
VERSION     DAOOLN010000860.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162378_length_1175_cov_3.116964"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1175)
                     /locus_tag="PLW80_10320"
     CDS             complement(<1..>1175)
                     /locus_tag="PLW80_10320"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPB66951.1"
                     /translation="KVIALEKSGRIGGTAVVTSGPMAVNAPSMVGNRIVDWDDPLLKK
                     KRVKEAGEPLVDAGALLRDWLQYTTVDGVQGAKEGIIRRVVDRSGQTLDWLTGFGFEF
                     SDARGFLGNQWALFSPFKGNKALTESFFDKAVARFEKEFGGRCLVETEATSLIIEGGR
                     IAGVRARKSDGTEVTVKAKAVILASGGFGGSDEMMEQYLGESWKMYGMEQNEGDGIRM
                     ALSAGAATYNIGMPPMSHFSAPSVILRSFDSDFDNDIPFGMVATSETLAVNKSGVRFI
                     NEANIGLDAYLGGSRFYTIYSKEQIDVLRARGFGFAATGRYLNRGGIKADAPLVNIDA
                     VIDAGVEAGFVYKAKSLSALAAAIAADNGVMKAANLERAVAEYGAGIATGKDVLGKP"
BASE COUNT          193 a          410 c          351 g          221 t
ORIGIN      
        1 ggggcttgcc caggacgtcc ttcccggtcg cgataccggc accatactcc gcgacggccc
       61 gctccaggtt agcggccttc atcaccccgt tgtcggccgc gatggccgcg gccagggccg
      121 agaggctctt cgccttgtac acaaagcccg cctcgacgcc ggcgtcgatg acggcatcga
      181 tgttgactag cggcgcgtcg gctttgatac cgccgcgatt caggtagcgc ccggtcgccg
      241 cgaagccgaa gcccctggct ctgaggacat cgatctgctc cttggaatag atcgtgtaga
      301 agcgcgagcc gcccaggtag gcatcgaggc cgatattggc ctcgttgatg aagcgaacgc
      361 ccgacttgtt caccgccagg gtctcgctgg tggccaccat cccaaagggg atgtcgttgt
      421 cgaagtcgga gtcgaagctc ctgaggatga ccgacggggc cgagaagtgc gacatcggcg
      481 gcataccgat gttgtaggta gccgcgccgg cgctcagggc catgcgtatg ccatctccct
      541 cgttctgctc catgccgtac atcttccacg actcgccgag gtactgctcc atcatctcgt
      601 cgctgccgcc gaacccgccg ctcgccaaga tgaccgcctt ggccttaacg gtaacctcgg
      661 tcccatcgga cttcctggcc cggacgcccg cgatgcggcc gccctcgata atgagagagg
      721 tcgcctcggt ctctaccagg caacggccgc cgaactcttt ctcgaaccgt gccacggcct
      781 tgtcgaagaa gctctcggtc agggccttgt tgcccttgaa gggggagaac agcgcccact
      841 ggttccccag gaagcccctc gcgtcggaga actcgaagcc gaagccggtc agccagtcca
      901 gggtctggcc tgatcggtcg acgacccgcc gtatgatccc ttccttggcg ccctgaacgc
      961 cgtcgacggt cgtgtactgg agccaatcgc gtagcagggc ccccgcgtcg accagcggct
     1021 cgccggcttc cttgacccgc ttcttcttca gtaacggatc gtcccagtcg acgatcctat
     1081 tcccgaccat gctcggagcg ttgaccgcca tcgggccgct ggtcaccacg gccgtgccgc
     1141 caatccgacc gctcttctcg agggctatga ccttg
//
LOCUS       DAOOLN010000861         1175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_162386_length_1175_cov_2.994643, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000861 DAOOLN010000000
VERSION     DAOOLN010000861.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162386_length_1175_cov_2.994643"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PLW80_10325"
     CDS             <1..814
                     /locus_tag="PLW80_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66952.1"
                     /translation="AAHRGKLIMEANVMASALTMLVPLVLLAGSRMTMGWRIVFPAML
                     VSVAATASFGLPATMRHLKSRDERADAGGGRLGGAFWRMWLIVFFGVSVEWAVSFWCM
                     TYLLGLPGNSRELAAAGTVLLGLSAVAGRFFSSRVSHRIPEKRLLYLMMLVVAAGFPL
                     YWLRANAATAFVGLALCGIGSSNFYPLGLSLAVGHAPGNAAKAGSLIPVASGSAIGLA
                     PFMLGRLADLTDMRTALWYIPIGVLLILAIALVDRAFERRTGLGAADRQRTE"
     gene            827..>1175
                     /locus_tag="PLW80_10330"
     CDS             827..>1175
                     /locus_tag="PLW80_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66953.1"
                     /translation="MPGGSVRRRMIAILATVGALPFVVFSMAFLSVIGDRLAEYSETV
                     STAALQAVIATARERAIEGPRRDLPALLLQAADEADGSRLTELLRDTLAPRPEYLALI
                     VLDAAGRVVASYPD"
BASE COUNT          153 a          419 c          396 g          207 t
ORIGIN      
        1 agcggcgcat cgcggcaagc tgatcatgga ggccaacgtg atggccagcg cgctgacgat
       61 gctggtgccg ctcgtgctgc tcgccggctc gcgcatgacg atgggctgga ggatcgtgtt
      121 cccggccatg ctcgtctcgg tagcggccac ggcttccttc ggcctaccgg cgacgatgcg
      181 ccacctcaag tcgcgcgacg agcgcgcgga cgccggaggc ggcaggctcg gcggcgcgtt
      241 ctggcgcatg tggctgatcg tgttcttcgg cgtatcggtc gaatgggccg tcagcttctg
      301 gtgcatgacc tatctgctcg gcctgcctgg caactcgcgg gaactcgccg cggccggcac
      361 ggtcctgctc ggcctgtcgg ctgtcgcggg gcgcttcttc tcgagccgcg tcagccacag
      421 gatacccgag aaacggctcc tctacctgat gatgctcgtc gtcgcggcgg gcttcccgct
      481 gtactggttg cgagctaacg cggcgacggc cttcgtcggg ctggcgctct gcgggatagg
      541 ctcgtcgaac ttctacccgc tcgggctgtc gctggcggtc ggccacgcgc cgggcaacgc
      601 cgccaaggcg ggctcgctga ttccggtcgc ctcgggctcg gccatcggcc ttgccccgtt
      661 catgctcggc cgcctcgccg acctcaccga catgaggacg gccctctggt acatacccat
      721 cggcgtgctc ctgatcctcg ccatcgccct ggtcgaccga gccttcgagc ggaggaccgg
      781 cctcggggcg gccgatagac agcgcacaga ataggcgtag gataccatgc caggcggtag
      841 cgtacggcgg cgtatgatag ccatcctcgc gacggtaggc gccttgccct tcgtcgtctt
      901 ctccatggcc ttcctgtcgg tgatcggaga ccggctggcc gagtactccg agaccgtgtc
      961 gacggcggcg ctccaagccg tgatcgccac ggccagggag cgcgccatcg agggcccgcg
     1021 ccgagacctg ccggcgctgt tgctgcaggc ggcggacgaa gccgacggct ctcggctgac
     1081 cgagctgctc cgcgacaccc tcgccccccg gcccgaatac ctggccctga tcgtactcga
     1141 tgccgccggc cgcgtcgtcg cctcctaccc cgaca
//
LOCUS       DAOOLN010000862         1175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_162411_length_1175_cov_2.698214, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000862 DAOOLN010000000
VERSION     DAOOLN010000862.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162411_length_1175_cov_2.698214"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="PLW80_10335"
     CDS             complement(<1..1118)
                     /locus_tag="PLW80_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HPB66954.1"
                     /translation="MNNGAEPQTLDPSKIQGVPEHKIYMALFEGLVGYDPKTSLATPG
                     VAESWTVSPDGTQVTFKLRKTTWSDGTKITAQTVVDSWLRTLDPATASEYAYMAGMVV
                     KGANEFNAGEAGKEAVQIRALDDYTFQVDLLGPMPYAVDMMAHYTFAILPMHVINAKG
                     EDWIKVENIVGNGPFKLAEWKPQESLTVVPNDKYWDAKTVKLSKITFLPIDDNLTAYN
                     KFKAGEVDWAHGIPLSLVDEIKLRPDYQVAPQVATYYYLFNVTRKPFDDVRVRKALTM
                     ALDMQELVDKVTKGGQLATSSMVPAMEGYTPAKGPTFNVEEARKLLAEAGFPDGKGFP
                     KTPILYNTSDGHKIIAEWVQESWKKNLNIEVSLVNQEW"
BASE COUNT          185 a          334 c          404 g          252 t
ORIGIN      
        1 ttccactcct ggttgaccag ggagacctcg atgttcagat tcttcttcca ggactcctgc
       61 acccactcgg cgatgatctt gtgaccgtcg ctggtgttat acaggatcgg ggtcttcggg
      121 aagcccttgc cgtccgggaa accggcctcg gcgagaagct tgcgagcctc ttctacgttg
      181 aaggtcgggc ccttggctgg cgtgtagcct tccatggccg ggaccatcga ggaggtagcc
      241 agctggccgc ccttggttac cttgtcgacg agctcctgca tgtcgagggc catcgtcagc
      301 gccttgcgga cgcggacgtc gtcgaacggc ttgcgggtca cgttgaacag ataatagtag
      361 gtggcgacct gtggggcgac ctggtagtcc gggcgaagct tgatctcgtc gaccaggctg
      421 agcgggatac cgtgggccca gtcgacctcg ccggccttga acttgttata ggcggtgagg
      481 ttgtcgtcga tcggaaggaa ggtgatcttg gagagcttga cggtcttggc gtcccagtac
      541 ttatcgttcg ggacgaccgt gagggactcc tggggcttcc actcggcgag cttgaagggg
      601 ccgttgccga cgatgttctc gaccttgatc cagtcctcgc ccttggcgtt gatgacgtgc
      661 atcgggagga tggcgaacgt gtagtgagcc atcatgtcca cggcgtaggg catcgggccg
      721 agcaggtcga cctggaaggt gtagtcgtcg agcgcgcgga tctgcacggc ttccttgccg
      781 gcctcgcccg cgttgaactc gttggcgccc ttgacgacca tgccggccat gtacgcgtac
      841 tcggacgcgg tggcgggatc gagggtgcgc agccagctgt cgaccacggt ctgggccgtg
      901 atcttggtgc cgtcggacca ggtggtcttg cggagcttga aggtgacctg ggtgccgtcg
      961 gggctgacgg tccagctctc ggctacgccg ggcgtggcga gagaggtctt gggatcgtac
     1021 ccgacaaggc cctcgaacag ggccatgtaa atcttatgct cgggaacgcc ctggatcttc
     1081 gagggatcga gcgtctgagg ctcggcgccg ttattcatta cgaactcgac gcctttggcc
     1141 gggcccttcc accgcaggag acgatcaggg tcgcc
//
LOCUS       DAOOLN010000863         1175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_162434_length_1175_cov_2.481250, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000863 DAOOLN010000000
VERSION     DAOOLN010000863.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162434_length_1175_cov_2.481250"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PLW80_10340"
     CDS             <1..730
                     /locus_tag="PLW80_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB66955.1"
                     /translation="RCVSVFDENKRFNPRYDESDTMTVEADYVLSSIGQAIDWGKLLD
                     GAKVELKPNKGPVADSITYQTAEPDVFVGGDVFTGPRFAIDAIAAGKEGAISLHRYVQ
                     KGQSLVMGRLKRNYVPLNKDNVDLDGYDNIPRQRAAHAPAKDAFKDVRGTFTPEQIKK
                     EAERCLSCGATFVDTTLCVGCGMCATKCKFDAISLERTFDGHGEDYRDLKPILIKSVL
                     KRKLRIAIRKPGRLLKSAFAPKQD"
     gene            801..1151
                     /locus_tag="PLW80_10345"
     CDS             801..1151
                     /locus_tag="PLW80_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HPB66956.1"
                     /translation="MGSLHELSVVIEVVRTVEEFARENGVTRIDKLVLQIGELSSMIP
                     QYIEDCFPCAVDGTSMVDTKLEIEILPGNGRCRSCGAMFNLVANKGVCPECGVKDAEV
                     ISGKEFMIKEIVAC"
BASE COUNT          256 a          350 c          373 g          196 t
ORIGIN      
        1 gcgttgcgtg tccgtcttcg acgagaacaa gcgctttaac ccgaggtacg acgagagcga
       61 cacgatgacg gttgaggccg actacgtgtt gtcgtccata ggccaggcta ttgactgggg
      121 caagctactg gacggcgcca aggtggagct gaaaccgaac aaggggcccg tggccgattc
      181 gatcacctat cagaccgccg agcccgacgt gttcgtaggc ggggacgtct ttaccggacc
      241 gcgcttcgcg atagacgcga tagccgccgg caaggaaggc gccatctccc tgcaccgcta
      301 cgtgcagaag ggccagagcc tcgtcatggg tcgcctcaag cgcaactacg tgcccctcaa
      361 caaggacaac gtggatctgg acggatacga caacataccc cgccagcgcg ccgcccacgc
      421 cccggccaag gacgccttca aggacgttcg cggtaccttt accccggagc agatcaagaa
      481 ggaagccgag cgttgcctga gctgcggcgc caccttcgtc gacacgaccc tgtgcgtcgg
      541 atgcggcatg tgcgccacca agtgcaagtt cgacgccata agcctggagc gtacgttcga
      601 cggccacggc gaggactacc gggacctgaa gcccatactc atcaagagcg tgctcaaacg
      661 caagctgagg attgcgatca ggaagcctgg ccggctgctc aagtcggcgt tcgcgcctaa
      721 gcaggactag gcctccaatc ggccagagac tattcggacg ggggacgatg gttcccccgt
      781 ccatcgtatc tacgggagag gtggggtcct tgcacgagct gagcgttgtc atagaggtcg
      841 taaggaccgt cgaggagttc gccagggaga acggcgttac ccggatagat aagctggtcc
      901 ttcagatagg cgagctatcg tcgatgatcc cccagtatat agaggactgc ttcccgtgcg
      961 ccgtcgacgg cacctccatg gtcgacacca agctggagat agagatcctg cccggcaacg
     1021 ggcgctgcag gtcctgcgga gctatgttca acctggtggc gaacaagggc gtctgtcccg
     1081 agtgcggcgt caaggatgcg gaagtcatct caggcaagga gttcatgatc aaggaaatcg
     1141 tcgcctgcta ggcgacgagc cgacgagccg acgag
//
LOCUS       DAOOLN010000864         1175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_162459_length_1175_cov_2.300000, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000864 DAOOLN010000000
VERSION     DAOOLN010000864.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162459_length_1175_cov_2.300000"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="PLW80_10350"
     CDS             <1..909
                     /locus_tag="PLW80_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB66957.1"
                     /translation="PTLRADHRGDPVRGLIGALMGVSGFCSKVLRVPAGRVLLAFIRK
                     KAGLGSVRLGVAGGGPLALDTAEFFDTIGFNLVQGYGMSENGPLIAVNLPEYKDNRSV
                     GLPVKRTLVRIADPGPDGVGEIQVQSPSMMLGYLDAPEATRAVITDDRWLRTGDLGYV
                     DRRGFIFITGRSKNIIVTEGGKNVYPEEIELRFEGSAWINEALVLGRKVSGLNAGEQV
                     IAVCVPNWDRVDAERGGVPRDEFALERVRDEVRRVNKSLPSFMKIIDFIVRNDEFEKT
                     STRKIRRFLYADYGRAPAPERPEVGR"
     gene            906..>1175
                     /locus_tag="PLW80_10355"
     CDS             906..>1175
                     /locus_tag="PLW80_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66958.1"
                     /translation="MSLDIAALALRFAVMAVATFLAIIVWSRVRDVSWMLIVVGIIAG
                     YGDILYSLLVEFGLVPDIGEAMGGLSFLAFVAPNLPWVFFSIAFMT"
BASE COUNT          191 a          366 c          388 g          230 t
ORIGIN      
        1 cccaccttac gcgccgacca tcgcggcgat ccggtccgcg gcctcatcgg cgccctgatg
       61 ggcgtctcgg ggttctgctc caaggtcctg cgcgtgccgg ccggacgcgt tctcctcgcc
      121 ttcatccgca agaaggccgg cctcggttcg gtcaggctcg gcgtcgccgg tggagggccg
      181 ctggcgctcg atacggcgga gttcttcgac actatcggct ttaacctggt ccagggctac
      241 gggatgagcg agaacgggcc gctcatcgcg gtcaacctgc ctgagtacaa ggacaaccgc
      301 tcggtgggtc tgccggtcaa gcggacgctt gtgcgtatag ccgatcccgg tcctgacggc
      361 gtcggagaga tccaggttca aagcccctcg atgatgctcg gctacctcga cgctccggaa
      421 gcaacccgag cggtcataac ggatgaccga tggctgcgta ccggggactt gggctacgtc
      481 gataggcggg gtttcatctt catcaccggc cgctccaaga acatcatcgt caccgagggc
      541 ggcaagaacg tgtatcccga ggagattgag ttgcgcttcg agggctcggc ctggataaac
      601 gaggcgttgg tgctcggccg caaggtctcc ggcctcaacg cgggcgagca ggttatagcc
      661 gtctgcgtgc cgaattggga cagggtcgac gccgagcgcg gaggggtgcc gcgcgacgaa
      721 ttcgcgctcg aacgcgtacg cgatgaggtg cgccgggtca acaagagcct cccatcgttc
      781 atgaagatca tcgactttat cgtacggaac gatgaattcg agaagacgag cacccgtaag
      841 atcaggcgct tcctgtacgc cgactacggg cgcgccccgg cgccggagcg gcccgaggtc
      901 ggccgatgag cctcgatatc gccgccctgg cgctgcgctt cgcggtgatg gcggttgcta
      961 ccttcctggc catcatcgtg tggtcgcgcg tgcgcgacgt gtcgtggatg ctcatcgtcg
     1021 tcggcatcat cgccgggtat ggagacatcc tctactcgtt attggtcgaa ttcggcctcg
     1081 tgccggatat cggggaggcg atgggcggct tgagcttcct ggccttcgtc gctccgaacc
     1141 tcccgtgggt attcttctcg atcgccttca tgacc
//
LOCUS       DAOOLN010000865         1174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_162611_length_1174_cov_2.730116, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000865 DAOOLN010000000
VERSION     DAOOLN010000865.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162611_length_1174_cov_2.730116"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1174)
                     /locus_tag="PLW80_10360"
     CDS             complement(<1..>1174)
                     /locus_tag="PLW80_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66959.1"
                     /translation="DLEAPPQGVPDDIADLLQSDDAALGDAALGDAFSSFLDDIPLGD
                     DQVAEPAPTDDFELPGGFGEESSPAGPPERETDDFTVPDSLLEDFGSLLETEEATEAA
                     PTDDAPTEEPIEPIEPIESLEATESETTGLDDFTVPDSLLDGAADETSQERPEMTSEA
                     VSANDDFSFDDLESLESEGDSEAAKPDDGFSIPDFDDMGLAEAEPEAPGSDEAAAPGP
                     DPEARGAESAGFDLGSESMEGDSFDQFDLGGGSPEVAVPEMGDSDFGGEFSDLGSESA
                     AADTFSLGGDWGGDFTIPGFEMGNEVKPTTAKPAASKEARPGKAPSSSLAGAFGSGEA
                     RASAERKARPVELTENQADALQDTLLSYPLNLRLAIEDIVANQKGTELQQSDLVWLL"
BASE COUNT          174 a          397 c          385 g          218 t
ORIGIN      
        1 gaggagccag accaggtcgg actgctggag ctcggtgccc ttctggttgg cgacaatgtc
       61 ctctatggct agccgaaggt tgagcggata ggataggagc gtatcctgga gcgcgtccgc
      121 ttggttctcg gtcagctcta ccgggcgggc cttcctttcg gcggaggccc tggcctcgcc
      181 ggaaccgaag gcgccggcca gggatgacga aggggctttg ccgggccgcg cttccttgct
      241 cgccgcgggc ttggcggtcg tcggcttgac ctcgttgccc atctcgaagc cggggatggt
      301 aaagtcgcca ccccagtcgc cgcccaggga gaaggtgtcg gccgccgccg actcgctccc
      361 caggtcggag aattcgccgc cgaagtcgct atcgcccatc tccggtacgg cgacttcggg
      421 agaccctccg cccaggtcga actggtcgaa ggagtcccct tccatggatt cggagccgag
      481 atcgaagccc gcggactcgg caccccgcgc ctcgggatca gggccgggcg ctgccgcctc
      541 gtcggaccca ggcgcttccg gttcggcctc cgccaggccc atgtcgtcga agtccggtat
      601 ggagaaaccg tcgtcgggct tggccgcctc cgaatcgcct tcgctctcga gcgactccag
      661 atcgtcgaag ctgaaatcat cgttcgcgct tacggcctcg ctggtcatct cgggccgctc
      721 ttgcgacgtt tcatcggcgg caccgtccag gagcgaatcc ggtaccgtga aatcgtctag
      781 ccccgtcgtc tccgactcgg tcgcctcgag ggactcgatg ggttctatcg gctcgatggg
      841 ttcctccgta ggagcgtcgt ccgtcggggc cgcttcggta gcctcttccg tctcgagcaa
      901 tgaaccgaaa tcctcgagca gcgagtccgg taccgtgaag tcatcggtct ctcgctcggg
      961 cggcccagcg ggggacgact cctctccgaa gccgccgggc aactcgaaat catcggtcgg
     1021 cgccggttcc gccacttggt cgtcgccgag cggtatatcg tccaggaacg aggagaacgc
     1081 gtcgcccaag gccgcgtcgc ccaaggccgc gtcgtcggac tgcaataggt ccgctatatc
     1141 gtcgggtacg ccctgcggcg gcgcctccag gtca
//
LOCUS       DAOOLN010000866         1174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_162627_length_1174_cov_2.537087, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000866 DAOOLN010000000
VERSION     DAOOLN010000866.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162627_length_1174_cov_2.537087"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PLW80_10365"
     CDS             <1..367
                     /locus_tag="PLW80_10365"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HPB66960.1"
                     /translation="RQEAKRTVGASDSPMTDALEALMAGPSADELGRGLISLIPSEAR
                     LLSAQVRGSTAYLNFNEAFMYNRYGIEGYAGQLKQIVYTATNFSTVKDVQILIEGQPR
                     DYLGGDGVYIGKPLSRASF"
     gene            377..793
                     /gene="arfB"
                     /locus_tag="PLW80_10370"
     CDS             377..793
                     /gene="arfB"
                     /locus_tag="PLW80_10370"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HPB66961.1"
                     /translation="MRDDLIEASVLGAAELEYARSSGPGGQNVNKVETKVRARVDLDR
                     VDGLSEAERERVKATLASRLDADGRLFVAVDLERSRARNEAIALSRLTELIAKAAKVP
                     KKRKPTRPTRASKERRLESKRSRAGTKVGRSRPTDD"
     gene            959..>1174
                     /locus_tag="PLW80_10375"
     CDS             959..>1174
                     /locus_tag="PLW80_10375"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66962.1"
                     /translation="MNQTRLHDWHLAAGAKMAPFAGYDMPISYPIGSVEEHLVTRRSV
                     GLFDIDHMGQVEVTGPGADAFVSRLVSA"
BASE COUNT          224 a          384 c          376 g          190 t
ORIGIN      
        1 ccgccaggag gccaagcgca ccgtcggcgc ctccgattcg ccgatgaccg acgcccttga
       61 agccctgatg gccggtccaa gcgccgacga gctgggccga gggctgatca gcctgatacc
      121 ctcggaagcg aggctcctgt cggcccaggt cagaggctcg acggcctacc tgaattttaa
      181 cgaggcattc atgtacaacc gctatggcat cgagggctat gcgggccagc tcaagcagat
      241 cgtctatact gccacgaatt tctccacggt caaggacgtc cagatactca tcgaggggca
      301 gccgcgcgac tatctcggcg gcgacggcgt gtacatcggc aaaccgctgt cgcgggcgtc
      361 cttctagggt ggcgccatgc gcgacgacct gatagaagcc tcagtgttgg gcgccgccga
      421 gctcgagtac gccaggtcgt ccgggccggg cggccagaat gtcaacaagg tggagacgaa
      481 ggtgcgcgcc cgcgtcgacc tggatcgcgt cgacggcctc tcggaggccg agcgcgagcg
      541 cgtcaaggcg acgctcgcct ccaggctcga cgccgacggc cgactcttcg tcgccgtgga
      601 cctcgagcgc tccagggctc ggaacgaggc gatcgccctg tcgaggctga ccgagcttat
      661 cgccaaggcc gccaaggtgc cgaagaagcg taagccgacg aggccgacac gagcctcgaa
      721 agagcggcgc ctggagtcca agcgttcgag ggccggcacc aaggtcgggc gctcgcggcc
      781 gaccgacgat tgagcgccga agccggatcg aagccagtcg tgcttcatta taattttgaa
      841 taatgaaacc tcgttttaac gagctgacct tgtcgatgga ttgctttcaa gctacaattg
      901 atccgttcga aacggccgac gccctgcgcg ccgcgggccg aaagaggagg acgcaatcgt
      961 gaatcagact cgcctgcatg actggcacct cgccgccgga gccaagatgg ctccgttcgc
     1021 cggctacgat atgcccatca gctatcctat aggctccgtc gaggagcacc tggtgaccag
     1081 gcgctccgtc gggctcttcg acatcgacca catgggccag gtcgaggtga ccgggcccgg
     1141 cgccgacgcc ttcgtctccc gcctcgtcag cgcc
//
LOCUS       DAOOLN010000867         1173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_162896_length_1173_cov_2.084079, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000867 DAOOLN010000000
VERSION     DAOOLN010000867.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162896_length_1173_cov_2.084079"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLW80_10380"
     CDS             complement(<1..181)
                     /locus_tag="PLW80_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66963.1"
                     /translation="MHHQTDLLAQYQNETAAITPFAQARMGLKPGQTVVKLDTYGIIC
                     VPYRLSMAGAVLVASF"
     gene            complement(215..1027)
                     /locus_tag="PLW80_10385"
     CDS             complement(215..1027)
                     /locus_tag="PLW80_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPB66964.1"
                     /translation="MNGSLYLVPVPIGDGDPADELAPRVLRIAAGLRDFVVENERSAR
                     RFLCRVMAQDALDASRFGVLDEHTRPEDVRALLAPLLAGRDVAILSEAGSPCVADPGA
                     ALVAEAAEAGIRVVPLPGPSSILMAVMASGLGGQRFAFAGYLPPDQAGREAALRALEA
                     DSRRQGSTQAFIETPYRNDAMVASAACALAPDTVFCAAIGLSTPEERIVRMTAAAWKA
                     GPVRIGKKPTVFLLKAADGNRGGASGRDRRGGSPFDRKAQPDRKAPPRKRGS"
BASE COUNT          176 a          408 c          395 g          194 t
ORIGIN      
        1 agaaggacgc gacgaggacc gcaccggcca tcgataggcg gtacggtacg cagatgatcc
       61 cgtaggtgtc gagcttgacg acggtctggc ccggcttcag gcccatgcgg gcctgggcga
      121 acggcgttat cgcggcggtt tcgttctggt attgggctaa caggtccgtt tgatgatgca
      181 tggctatacc agtgtaacct tcaatgcgga atcgtcaaga tccgcgtttg cggggcggcg
      241 cctttcgatc gggctgcgcc tttcgatcga aaggcgatcc tccgcgcctg tcccggccgc
      301 tggccccgcc ccggttgccg tcggcggcct tcagcaggaa taccgtcggc ttcttgccta
      361 tgcgcacggg cccggctttc caggcggcgg cagtcatgcg aacgatgcgc tcctccggtg
      421 tcgataggcc gatcgcggcg cagaacacgg tatcgggagc cagtgcgcag gccgcgctgg
      481 cgaccatggc gtcgttgcgg tatggcgtct cgatgaaggc ctgcgtcgaa ccctgccgcc
      541 tcgagtcggc ctcgagcgcg cgcagggcgg cctccctgcc ggcctggtct ggcggtagat
      601 agccagcgaa cgcgaagcgc tggcccccga ggcccgaggc catgacggcc atcaggatcg
      661 agctcgggcc gggcaggggg acgactctga tccccgcctc ggccgcctcc gccaccaggg
      721 ccgctcccgg gtcggcgacg caggggctac cggcctccga cagtatggcc acgtcccgcc
      781 cggccagcag gggcgccagc agcgcccgca catcctcggg ccgcgtatgc tcgtccagca
      841 cgccgaagcg cgacgcatcg agggcgtcct gcgccatgac gcggcatagg aagcgcctgg
      901 cgctgcgctc gttctcgacg acgaagtcgc gaaggccggc cgctatcctg agtaccctcg
      961 gggccagttc gtccgccggg tcgccgtcgc ctatcggcac cggcaccaaa tatagtgagc
     1021 cgttcatctc ccgccttcct ggacgttggc caggaacgac atgacgtacg cgccgaagcg
     1081 ttcggccagc ctggccggga aaccagcctc gatcgcctgt tcgcgcgcgg cctcctgggc
     1141 ctcctcgacg tcctttacgg tcaacggcaa cct
//
LOCUS       DAOOLN010000868         1172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_163012_length_1172_cov_3.483438, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000868 DAOOLN010000000
VERSION     DAOOLN010000868.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163012_length_1172_cov_3.483438"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PLW80_10390"
     CDS             complement(<1..333)
                     /locus_tag="PLW80_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPB66965.1"
                     /translation="MQNDIRRLLDAAASAAKEAAWVGLRRRRTSASYYMARDGAFDQA
                     IGGLDDGVMVEVLYDGQFAYGATPDLSPEGVARAAASAMVAAKAASGKGVHRFDTSVR
                     PATRVAYRT"
     gene            452..>1172
                     /locus_tag="PLW80_10395"
     CDS             452..>1172
                     /locus_tag="PLW80_10395"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB66966.1"
                     /translation="MRVVIIGAGMIGLHIARELIDEGRDVVLVEKDAEAARRAGDELD
                     CLVVNEDGSRPETLRKAGVSGSAWFLALTGSDEVNIVACGLVAAECPDVHTVARVENP
                     FYSSLSAAQRRAFGLSVLINPAAETAAAIGRIVDEGFAEDLIPLHDGRLHLRTVLAGS
                     APAFVGRPLRDAKAEAGNGVLVAAVVRDKGIVVPDGNFIVEATDLLYLLGEPATLDGF
                     LGQVAGVKAGSKRVMVIGATAI"
BASE COUNT          180 a          384 c          409 g          199 t
ORIGIN      
        1 cgtccggtac gcgacgcgcg tcgccggcct gacggaggtg tcgaaacggt ggacgccctt
       61 gcctgacgcg gccttggcgg cgaccatggc cgacgcggcc gcccgagcca cgccctctgg
      121 cgacaggtcg ggggtggcgc cgtaggcgaa ctggccgtcg tagagtacct cgaccatgac
      181 gccgtcgtcg aggccgccta tggcctggtc gaaggctcca tcacgggcca tgtaatatga
      241 ggcgctcgtg cgccgccgcc tgagcccgac ccatgcggct tccttggcgg ccgaggcggc
      301 cgcgtccagg agccggcgaa tatcgttctg catggctctc tcctttattg cgatctgcgt
      361 tgcgtcagcg gcgacgagtg taacgttcaa ataagttgaa cgtcaaggtg aaagcttatc
      421 taaaatctaa tttgggcgta tagtggccgc gatgcgagtt gtaataatcg gggccgggat
      481 gatcggcctc catatagcgc gggagctcat agacgagggt cgggacgtcg tactcgtcga
      541 gaaggacgcc gaggcggcca ggcgggccgg cgacgaactc gattgcctcg tggtgaacga
      601 ggatggcagc cgtcccgaga ccctgcgcaa ggctggagtc tcgggctcgg cgtggttcct
      661 ggccctgacc ggcagcgacg aggtgaacat cgtcgcctgc gggctcgtgg cggccgagtg
      721 ccccgacgtc catacggtgg ccagggtgga aaacccgttc tattcgtcgc tctcggccgc
      781 ccagcgccgc gcgttcggcc ttagcgtgct gatcaacccg gcggccgaga ccgccgccgc
      841 cataggccgc atcgtcgacg agggcttcgc cgaggacctg ataccgctcc acgacggtcg
      901 cctgcacctg cgcacggtct tggccggctc ggcgccggct ttcgtcggca ggccgctccg
      961 cgacgcgaag gccgaggcgg ggaacggcgt gctcgtggct gccgtggtcc gcgacaaggg
     1021 catcgtcgtg cccgacggca acttcatcgt cgaggcgacc gacctcctgt acctgctcgg
     1081 cgagcccgcc accctcgacg gcttcctcgg ccaggtggcc ggggtcaagg ccggttccaa
     1141 gcgcgtcatg gtcatcggcg ccacggctat cg
//
LOCUS       DAOOLN010000869         1169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_163672_length_1169_cov_3.387792, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000869 DAOOLN010000000
VERSION     DAOOLN010000869.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163672_length_1169_cov_3.387792"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="PLW80_10400"
     CDS             complement(<1..1089)
                     /locus_tag="PLW80_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66967.1"
                     /translation="MAMGILQAILSFFRGLLGLEMGAKGELKRIYSVISPIKPPYYRF
                     KNNLVMTGFAQDLYLFCQAIKPLMDLADRTLAHPDLRVSRRYFDYLVDLELPPQELEK
                     KESFAYDGMKARIENAVRDDEEQEAVAKDFQHFLKSLEALSDGPLNTDLHEVERFIEV
                     CRHDWERTLGFFDPGASLDDSRYRPDFQPCAGEQVLPELIDAYYLLIGFSFSENLLRK
                     TMKVFERHAPATAASQAGKITKLFSTLNKILSYRLSSDNLLALIRLTKRDAIYEPDTK
                     RQRTDHIEEYRKRVVTQYERDRERLLRERHENAVSLDIRELFGGTEVLVLDGYNEEVD
                     SYLRRESPNGFTHVKPMGILKTFVKGVFE"
BASE COUNT          206 a          326 c          392 g          245 t
ORIGIN      
        1 ctcgaacacg cccttcacga aggtcttgag tatgcccatc ggcttgacgt gggtaaagcc
       61 gttcgggctc tctcgccgca ggtaggagtc gacctcctcg ttatagccgt cgaggacgag
      121 cacctcggtg ccgccgaaca gctcgcgaat atccagggat acggcgttct cgtggcgctc
      181 gcggagcagc ctttcccggt cgcgctcgta ctgcgtgacg acgcgcttgc ggtattcctc
      241 gatatggtcc gtcctctgcc gcttggtatc gggctcgtag atggcgtcgc gcttggtcag
      301 cctgatcagg gcgagcaggt tatcgctgga taggcggtac gagagtatct tgttcaaggt
      361 cgagaatagc ttggttatct tgccggcctg gctggcggcg gtcgccggcg cgtgccgctc
      421 gaagaccttc atcgtcttcc ggagcaggtt ctccgagaag gagaagccga tgagcaggta
      481 gtaggcgtcg atgagttcgg gcaggacctg ctctccggcg cagggctgga aatcgggtcg
      541 gtagcgggag tcgtcaagac tggcgccggg atcgaagaag ccgagggtgc gctcccagtc
      601 gtgcctgcag acctcgatga agcgctcgac ctcgtgcagg tcggtgttga gaggaccgtc
      661 cgacagcgct tcgaggcttt tgaggaaatg ctggaagtcc ttggccacgg cttcctgttc
      721 ctcgtcgtcc ctgacggcgt tctcgatgcg ggccttcatg ccgtcgtagg cgaagctctc
      781 cttcttctca agctcctggg gcggcagctc gaggtcgacg aggtagtcga aatagcgtct
      841 ggagacgcgc aggtccggat gggccagggt ccggtcggcc agatccatga gcggtttgat
      901 cgcctggcag aacaggtaca gatcctgcgc gaagccggtc atgacgagat tgttcttgaa
      961 gcggtaatac ggcggcttga tcggcgagat gaccgagtag atgcgcttca gctcgccttt
     1021 cgcgcccatc tcgagaccga gaaggccgcg gaagaacgat agaattgcct gtaggatgcc
     1081 catggccatc tattctacgg caggatgggc tcggcgacaa gcgtgagcgg ccccgggtgt
     1141 tacgaacctt attgatcccc ggcgatgaa
//
LOCUS       DAOOLN010000870         1169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_163816_length_1169_cov_1.675943, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000870 DAOOLN010000000
VERSION     DAOOLN010000870.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163816_length_1169_cov_1.675943"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PLW80_10405"
     CDS             complement(<1..664)
                     /locus_tag="PLW80_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66968.1"
                     /translation="MKRTSTAAIVALLLVAAATAAFAQPEVSEKKEIAIFSLGFYGWD
                     IPRETLGTIDIDIQRVFLDLGRFTIFGMEKRFSTSDVDQFITILKQMKESTFVLPEKY
                     QFGEAFLTEADFNRLVGSFIIAVPVVTSYNSQYVDGKEWRTDIKTNVSFINVADGTMI
                     GIANVETQGSSRETQYTSVKEAIDGIPMQLQYEIRKISQFQNVTRVLASGFGGVDIQL
                     GSN"
     gene            complement(818..>1169)
                     /locus_tag="PLW80_10410"
     CDS             complement(818..>1169)
                     /locus_tag="PLW80_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66969.1"
                     /translation="GPMADAALSDESLAAHGFKAVAVFEPDPALIGASVGQDGARALP
                     LSKLPDLSRRMGVKIAILAVGGAWAQAAADGLKDSGIEAVLDLSPAHPQFPAGVRSVR
                     ADFGAAIAAPAAGL"
BASE COUNT          203 a          354 c          398 g          214 t
ORIGIN      
        1 tgttggagcc gagctggata tcgacgccgc cgaagccgga ggcgagcacc cgcgtgacgt
       61 tctggaactg ggatatcttg cggatctcgt actgcagctg catcgggatg ccgtcgatgg
      121 cttccttgac cgacgtatac tgagtttcgc ggctcgagcc ttgagtctcg acgttggcga
      181 tgcctatcat cgtgccatcg gccacgttga tgaagctgac gttcgtcttg atgtcggtgc
      241 gccattcctt gccgtcgaca tactggctgt tgtagctcgt gacgaccggc accgcgatga
      301 tgaacgagcc gacgaggcga ttgaagtcag cctcggtcag gaacgcctcg ccgaactggt
      361 acttctctgg cagcacgaag gtggattcct tcatctgctt caggatggtg atgaactggt
      421 cgacgtcgct cgtggagaaa cgcttctcca tgccgaagat ggtgaagcgg ccgaggtcga
      481 ggaagacccg ctggatgtcg atgtctatcg tgccgagcgt ctcccgcggg atgtcccagc
      541 cgtagaagcc gagcgagaag atggctatct ctttcttctc gctgacctcg ggctgcgcga
      601 aggccgccgt ggcggcggcg acgagtagca gggcgacgat agccgccgtc gatgtacgtt
      661 tcatgatccc tcctgatggc cgagtgtgcc tagctcgact agtatagagc atgacgacgg
      721 cttttgcgag cggaagagac gagggcggag gagacgagga acctcggcgc tagcgaggac
      781 gggcaacgac gagagcggcg cccggaggcg ccgtatccta tagtcctgcc gccggcgcgg
      841 caatggccgc cccgaagtcc gcccggaccg atcgtacgcc ggccgggaac tgcgggtgcg
      901 ccggcgatag gtcgagcacc gcctcgatgc ctgagtcctt aagcccgtcc gcggccgcct
      961 gggcccaggc cccgccaacg gcgagtatgg ctatcttgac gcccatgcgc ctggacaggt
     1021 cgggtagctt cgacaggggc agggccctcg cgccgtcctg gccgaccgac gcgcctatca
     1081 gggccggatc cggctcgaag acagctaccg ccttgaagcc gtgggccgcg aggctctcgt
     1141 cggacagggc cgcgtcggcc atagggccg
//
LOCUS       DAOOLN010000871         1168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_163881_length_1168_cov_3.431267, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000871 DAOOLN010000000
VERSION     DAOOLN010000871.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163881_length_1168_cov_3.431267"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1168)
                     /gene="pgi"
                     /locus_tag="PLW80_10415"
     CDS             complement(<1..>1168)
                     /gene="pgi"
                     /locus_tag="PLW80_10415"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625632.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPB66970.1"
                     /translation="TPGRLASRTLPAGGGLSYSYAAMDVDDGTIASLAAAATELGLVD
                     QYRALASGEVMNVGESRMVLHHLARGTPCGPVVKGGRDYRAFYEGERSRAAAFAAMVR
                     SGELRGSTGKRFERVVQIGIGGSDLGPRALYLALERWAAERGELALEASFISNVDPDD
                     ANGVLSGCDQETSLFVLVSKSGTTQETLANEALVRSWLGRRPGVDASRHIVAVTSETS
                     PLARNAGYLDSFYIDDYIGGRYSATSAVGAVVLSLALGPDAFKALLDGAADADRLALE
                     PDPTKNAALLDAMLGIYERNVLGRPSVAVLPYSQALARFPAHLQQLDMESNGKRVDRY
                     GKPVTIPGGPAVFGEPGTNGQHSFYQLIHQGTDVVPLQFIGFRKNQSGFDPEFQG"
BASE COUNT          167 a          384 c          430 g          187 t
ORIGIN      
        1 gccctggaat tcggggtcga agccgctctg gttcttacgg aagcctatga actgcagcgg
       61 cacgacgtcg gtgccctggt ggatgagctg gtagaacgag tgctggccgt tcgtgcccgg
      121 ctcgccgaat accgccgggc cgcccgggat cgtcacgggc ttaccgtagc ggtcgacccg
      181 tttgccgttg gattccatgt cgagctgctg gaggtgggcc gggaaacgcg ccagtgcctg
      241 ggagtagggc aagaccgcta cgctcgggcg gccgaggacg ttccgttcgt agatgccgag
      301 catggcgtcg agcagggccg cgttcttggt aggatccggc tcgagcgcca gtcggtcggc
      361 gtcggcggcg ccgtcgagca gggccttgaa ggcgtcgggg ccgagggcca gcgataggac
      421 gacggcgcct accgcgctcg tggcgctgta gcggccgcct atgtagtcgt cgatgtagaa
      481 cgaatcgagg tagccggcgt tgcgcgccag agggctcgtc tcgctggtta cggcgacgat
      541 atgccgcgac gcgtcgacgc cgggcctcct gccgagccag gaccggacga gagcctcgtt
      601 cgcgagagtc tcctgggtgg tgccgctttt cgacacgagt acgaacaggc tcgtctcctg
      661 gtcgcagccg gacagcacgc cgttcgcgtc gtcggggtcg acgttgctga tgaaggacgc
      721 ctcgagcgcc aattcgccgc gctcggcggc ccagcgttcg agggccaggt acagcgctcg
      781 cggccccagg tcggagccgc ctatgcctat ctgcacgacg cgctcgaagc gcttgccggt
      841 cgagccgcgt agctccccgg aacggaccat cgcggcgaag gccgcggcgc gagagcgctc
      901 tccctcgtag aaggcccgat agtcgcggcc gcccttgacg acggggccgc agggagtccc
      961 gcgggccagg tggtgcagca ccatgcggct ttcgccgacg ttcatcacct cgccggaggc
     1021 cagggcccgg tactggtcga ccaggccgag ctcggtcgcc gccgccgcca gcgaggctat
     1081 cgtcccgtcg tcgacgtcca tcgccgcgta ggagtagctg aggcccccgc cggctggcag
     1141 cgtcctggac gcgaggcgcc ccggggtt
//
LOCUS       DAOOLN010000872         1167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_164124_length_1167_cov_2.679856, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000872 DAOOLN010000000
VERSION     DAOOLN010000872.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164124_length_1167_cov_2.679856"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PLW80_10420"
     CDS             complement(<1..591)
                     /locus_tag="PLW80_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HPB66971.1"
                     /translation="MEYEFEGRTEEEAIEIAARELGIARDEFDVEIMESQRGGFLKKG
                     KTRIRVQVRGAPAAPAPRKRPAERPDARNQASVRGDADAAPRTVANVEVDGEFERKLL
                     DFVQTTVEKMGYTCKASILFREELKLGIRLDSDSAAILIGKKGKNLDALQLLANVYAG
                     RLGREDLRVILDSENYRIRREEAIVKMAYEVAERVRS"
     gene            complement(624..>1167)
                     /gene="yidC"
                     /locus_tag="PLW80_10425"
     CDS             complement(624..>1167)
                     /gene="yidC"
                     /locus_tag="PLW80_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HPB66972.1"
                     /translation="DMADFYKREGYNPMSGCLPLLIQFPIFIAMYSLFNNHFDLRGAS
                     FIAGWINDLSLPEAVYTFPTINLLIWKVSAIRVLPIVYVISQLLYGKLMQSQPASGQN
                     AGQMKFMMYGMPIMFFFILYDVPSGLLVYWISSNILTAAQQLVINKVIHKKRLATEAA
                     APQAKIVPIKSGKGGKKGRK"
BASE COUNT          212 a          359 c          347 g          249 t
ORIGIN      
        1 cgaacggacc cgttcggcga cctcataggc catcttgacg atggcttcct cgcgccggat
       61 cctgtagttc tccgaatcca gtatcacgcg caggtcctcg cggccgagcc tgccggcgta
      121 tacgttcgcc aggagctgga gcgcgtcgag gttcttaccc ttcttgccta tcaggatggc
      181 ggcgctgtcg ctgtccagcc tgatgccgag cttcagttcc tcgcggaaca ggatgctcgc
      241 cttgcaggta tagcccatct tctcgaccgt cgtctgaacg aaatcgagca acttccgctc
      301 gaactcgccg tcgacctcga cgttcgcaac cgttcgaggc gccgcatcgg cgtcgcctcg
      361 aaccgacgcc tggttccgcg cgtccggcct ctcagccggg cgcttacgcg gagccggagc
      421 ggccggggct ccccggacct gtactcgaat ccgcgtcttg cccttcttga ggaagccgcc
      481 gcgctgagac tccatgatct ccacgtcgaa ctcgtcacgg gcgatgccca gctcgcgggc
      541 ggcgatctcg atggcctctt cctcggtacg gccttcgaac tcgtattcca tattaggact
      601 cctataaaga gaggttttcg tagttacttg cgccccttct tgccgccctt tccgctcttt
      661 atgggaacga tcttggcctg gggagccgct gcctcggtcg cgaggcgctt cttgtggata
      721 accttgttga taacgagctg ctgagccgcg gtcaggatgt tcgacgagat ccagtacacg
      781 agcaggccgg agggcacgtc gtacaggatg aagaagaaca tgatcggcat gccgtacatc
      841 atgaacttca tctgcccggc gttctgcccg gatgccggct gggactgcat cagcttgccg
      901 tacaggagct ggctgatcac gtagacgatc ggcagcacgc gtatggccga gaccttccag
      961 atgagcagat tgatcgtcgg gaacgtgtac acggcctcgg ggagcgatag gtcgttgatc
     1021 catcccgcta tgaacgaagc gccgcgcagg tcgaaatggt tgttgaacag cgagtacatg
     1081 gcgatgaata tcgggaattg aatgagcagc ggcagacagc ccgacatcgg gttgtagcct
     1141 tcgcgcttat agaagtcggc catatcc
//
LOCUS       DAOOLN010000873         1167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_164139_length_1167_cov_2.532374, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000873 DAOOLN010000000
VERSION     DAOOLN010000873.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164139_length_1167_cov_2.532374"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="PLW80_10430"
     CDS             complement(<1..871)
                     /locus_tag="PLW80_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3029 family protein"
                     /protein_id="HPB66973.1"
                     /translation="MSASLSVITDRSLTYQQKVAALAKVGEETVRPLSIDPETQALRD
                     AGVVCDLFEGSAPYRPRYVVPDYERFMRRGSAFLGLEPPRDIWEATTALLVLYRHVPS
                     ITTFPVYLGDLDALLEPFVRDEAEARKAIGLFLLSIDRTLNDSFCHADIGPDATTAGR
                     LILELSREQKNAVPNISLKYDPDRTPDDFAILAAATALEVAKPSFANHRLFASEFEAM
                     GLGDYAIASCYNGLSKGGGSCTLVRMNLAALSPRSKGLDDFMDRVLPDAVERQLSYMD
                     ERVRFLVDESGFFE"
BASE COUNT          202 a          360 c          407 g          198 t
ORIGIN      
        1 actcgaagaa gcccgactcg tcgaccagga agcgcacccg ctcgtccatg tacgagagct
       61 gccgctcgac ggcgtcgggc aggacccggt ccatgaagtc gtccaggccc ttcgagcgcg
      121 gcgacagggc cgcgaggttc atcctgacga gcgtgcacga gccgccgccc ttggacaggc
      181 cgttgtagca cgaggctatc gcgtagtcgc ccaggcccat cgcctcgaac tcggaggcga
      241 acaggcggtg gttcgcgaag ctcggcttgg ccacctcgag cgccgtcgcc gcggcgagga
      301 tcgcgaagtc gtccggcgtg cgatcggggt cgtacttgag gctgatattc ggcaccgcgt
      361 tcttctgctc gcgagacagc tcgagtatca gccgaccggc cgtggtggcg tcgggcccga
      421 tgtcagcgtg gcagaacgag tcgttcagcg tgcggtctat cgacagcagg aacagcccga
      481 tggccttgcg ggcctcggcc tcgtcacgca cgaagggctc gagcagggcg tcgaggtcgc
      541 cgaggtagac ggggaaggtg gtgatgctcg gcacgtgccg gtacaggacg aggagggccg
      601 tcgtcgcctc ccagatgtcg cgcggcggct cgagcccgag gaacgcggat ccccgccgca
      661 tgaagcgctc gtagtcgggc accacgtagc gcggccggta cggcgccgag ccctcgaaga
      721 ggtcgcagac gacgccggcg tcgcgcaggg cctgggtctc gggatcgatc gaaagggggc
      781 ggacggtctc ctcgccgacc ttggcgaggg ccgcgacctt ctgctgatag gtaaggctgc
      841 gatcggtaat gaccgataat gacgcgctca tggctatact cccgtgtaga gtcgttgaag
      901 ctctggaagt ttaaccgcgc cggttttgac ggcccgcgta cggggccgct ccgctgggtg
      961 cgcgccgccc gacctgacgc gcgtgctaac cggggttctt tatcgcgtcg acgcgcgatg
     1021 ttgacagaat tataccccgt cgccgattca acagcaagta ccgcgggttc ccgagcgctc
     1081 cgttatgcac agaggcgggt gtccgatcga gtcgaatgcg gacaggattt atatgataga
     1141 actaggctat cggcatctat aatcgct
//
LOCUS       DAOOLN010000874         1167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_164213_length_1167_cov_1.911871, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000874 DAOOLN010000000
VERSION     DAOOLN010000874.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164213_length_1167_cov_1.911871"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..1016
                     /locus_tag="PLW80_10435"
     CDS             105..1016
                     /locus_tag="PLW80_10435"
                     /inference="COORDINATES: protein motif:HMM:NF015629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HPB66974.1"
                     /translation="MFDLDDAILEEMLFSMENQDIDFMVDASTGAVVVMNDDALDSGL
                     DSLDEGADLLPPPDWTSADGYGLMEAFAAGVGDPIARTSLAAALSRGRGVFKAFKTAI
                     EAYPDIARRWYDYKRCAMAKRVAEWYDEARVARGLERLGPEPEDDDLLADDFSFRIAG
                     REAWLECQPLFRRGLEEALGAFPEPLVEYEYTAIEREVAEGGKEGLVIVLAEAVAGAI
                     AGVAAARRVYVADGSFGKLTYLYVAPEHRRLGLGRRLAEAARERLAKDGIPRFVVDMP
                     FVPAGFGSSMKAFGYEPFGTRYLKTDD"
BASE COUNT          200 a          397 c          396 g          174 t
ORIGIN      
        1 tatatggaga tcggctcgca tcgttgtacc ctgggtagta tactaacccg cgaggcgagc
       61 cgcggacaag cagaagaacg cggcgcccct atccggagga cctaatgttc gacctagacg
      121 acgcgatctt agaggaaatg ctgttctcga tggagaacca ggacatagac ttcatggtcg
      181 acgcctcgac cggcgccgtc gtcgtgatga acgacgacgc gctcgactcg ggcctggact
      241 cgctcgacga gggcgcggac ctgctaccac cgccggactg gaccagcgcc gacggctacg
      301 gacttatgga ggccttcgcg gccggcgtcg gagacccgat agccaggacg agtctcgccg
      361 ccgccctatc gcgagggcga ggggtcttca aggccttcaa gacggcgatc gaggcctacc
      421 ccgacatcgc gcggcgctgg tacgactata agcgctgcgc gatggctaag cgcgtcgccg
      481 agtggtacga cgaggcgagg gtggcccgcg gcctcgagcg cctcggcccg gagcccgagg
      541 acgacgacct cctggccgac gacttctcgt tcaggattgc cggccgcgag gcgtggctcg
      601 aatgccagcc ccttttcaga cgcggcctcg aggaagcgct cggcgcgttc ccggagcccc
      661 tggtcgagta cgagtacacc gccatcgagc gggaggtagc cgagggcggc aaggaggggc
      721 tcgtgatcgt gctagccgag gccgtggcgg gggcgatagc cggcgtggcc gcggcgcgac
      781 gggtctacgt ggccgatggt tccttcggca agctaaccta cctgtacgtg gcgccggagc
      841 accgcaggct tggcctcgga cgtcgcctgg ccgaggccgc acgagagcgc ctggccaagg
      901 acggcatacc gcgcttcgtg gtagacatgc ccttcgtgcc ggccggcttc ggctcctcga
      961 tgaaggcctt cggctacgag cccttcggca cgcgatacct gaagaccgac gactgacggc
     1021 cacccctcgc tgacgacggg ctcagcgaga gccttagcgc gcgccgtccc agccgtaacg
     1081 gccgagatcc acccggccat cgcgacctac ctccacgccc tcgtcgacca gccgcgcctc
     1141 ctgctcctcg aagccgtagc cgcggcg
//
LOCUS       DAOOLN010000875         1165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_164584_length_1165_cov_2.546847, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000875 DAOOLN010000000
VERSION     DAOOLN010000875.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164584_length_1165_cov_2.546847"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="PLW80_10440"
     CDS             <1..597
                     /locus_tag="PLW80_10440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPB66975.1"
                     /translation="TGTEIHVAIAGRYAGRIGVGDSLKPSGRTALDGLRALGVRRVAM
                     LTGDSAASAERVAAEAGIAEVHHGLLPADKLERLEAILGQEQGRGSVVFVGDGVNDAP
                     VLARADAGIAMGGGSDAAVESADVVLIGGDPSRVAEAIDRARRTRRIVAQNIAFALVV
                     KLAFLGLGAIGLAAMWEAVIADVGVALLAVLNSTRALK"
     gene            678..1103
                     /locus_tag="PLW80_10445"
     CDS             678..1103
                     /locus_tag="PLW80_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HPB66976.1"
                     /translation="MRKTIVVVDGMGGGLGAQLIAKTRELIGDAAEIVALGANSCATE
                     RMLKAGADRGASGENAVRVSVGLGDVVLGPIGVIAPNAMMGEITPAMAEAVFGARGRL
                     VLVPAAQRHFTLVGVEKLPMAALIAAAAAEAVAALGLGG"
BASE COUNT          161 a          390 c          456 g          158 t
ORIGIN      
        1 accggcaccg agatccacgt ggccatagcc gggcgctacg ccggccgtat cggcgtcggc
       61 gactcgctca agccgagcgg cagaaccgct ctcgacggcc tgcgcgctct cggcgtgcga
      121 cgagtggcga tgctgaccgg cgactcggcc gcgtcggccg agcgggtggc cgcggaagcc
      181 ggcatagccg aggtgcacca cgggctcctt ccggcggata agctcgagcg gctcgaggcg
      241 atactcgggc aggaacaggg ccgcggctcg gtcgtcttcg tcggcgatgg cgtgaacgac
      301 gcgccggtgc tggcccgcgc ggacgccggc atcgcgatgg gcggcggctc ggacgccgcg
      361 gtggagagcg ccgacgtcgt gctgataggc ggcgacccgt cgcgggtggc cgaagctatc
      421 gaccgggcca ggcgcacgag gcgcatcgtg gcgcagaaca tcgccttcgc gctcgtcgtc
      481 aagctggctt tcctgggcct cggcgcgata gggctcgcgg cgatgtggga agcggtcata
      541 gcggatgtcg gcgtggccct gctcgcggtg ctcaactcga ctcgggcgct caagtagacc
      601 ggaattgcgg ctacgcgctc gagggctcgc gggacgggcg gcttgaccct ggctcgcctc
      661 gcggctaccc tcgggccatg aggaagacga tcgtcgtggt ggacggcatg gggggcggcc
      721 tcggggccca gctcatagcc aagaccaggg agctgatagg cgacgcggcc gagatagtgg
      781 ccctcggcgc gaactcgtgc gccaccgagc gcatgctcaa ggccggcgcc gaccgcggcg
      841 cctccggcga gaacgcggtg cgcgtctcgg tcgggctcgg cgacgtagtg ctcggcccga
      901 tcggcgtcat agcgccgaat gccatgatgg gcgagatcac gccggcgatg gccgaggccg
      961 tcttcggcgc gcggggccgt ctggtgctcg tacccgcggc ccagcggcac tttaccctcg
     1021 tcggcgtcga gaagctgccg atggccgcgc tgatagcggc ggcggcggcc gaggccgtcg
     1081 cggccctggg cctcggcggc tgacaggaaa accgctacgg ggcgccggga gcggccccgt
     1141 caccagcggg cgtatcggcg acctg
//
LOCUS       DAOOLN010000876         1164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_164820_length_1164_cov_2.522994, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000876 DAOOLN010000000
VERSION     DAOOLN010000876.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164820_length_1164_cov_2.522994"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /gene="rsgA"
                     /locus_tag="PLW80_10450"
     CDS             complement(<1..551)
                     /gene="rsgA"
                     /locus_tag="PLW80_10450"
                     /inference="COORDINATES: protein motif:HMM:NF015170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase RsgA"
                     /protein_id="HPB66977.1"
                     /translation="MSLGTVLSGANNSFSVLAEDGSVHYCALKGKKLKGAEGFYNPLA
                     AGDVVEWEPASAGTGMILSLRPRKSLFWRYNEKGKAIQAIAANMDLVVCVTSAGLPPF
                     RPRFIDRVSLEPASEGIPLIIALNKSDLGIDDDMAERMADYRRIGFETMELSAGTGAG
                     VDALRERVAGRTSVFVGQSGVGK"
     gene            complement(548..>1164)
                     /locus_tag="PLW80_10455"
     CDS             complement(548..>1164)
                     /locus_tag="PLW80_10455"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HPB66978.1"
                     /translation="VDAIRADTPGLIVVGTVPAIKPAAALSRVRRIGVVATEAAVAAD
                     YLTGLASAWAADCELVRIGDAGLVDFVERRLLGSTRDERLRAVAPSVRAMLERGVDVI
                     VLGCTHFLHLSEEFGAVAGPGVSIVDSRDGVAARLASRLDEEGLRGSVGARRTGGCPD
                     MRDAESGRPGEPSDRLFLTGAGDPGPVYGGFAERFGLSWAGCLP"
BASE COUNT          191 a          408 c          389 g          176 t
ORIGIN      
        1 ctcttgccga cgccggactg tccgacgaac acgctcgttc ggccggccac gcgctcccgc
       61 aaggcgtcaa ccccagcgcc cgttcccgcg ctcagctcca tcgtctcgaa gccgatgcgc
      121 cgataatcgg ccatgcgctc ggccatatcg tcgtcgattc ccagatccga tttgttgagc
      181 gctatgatca gcgggatgcc ttcggaggcg ggttcaagcg atacgcggtc tatgaatctg
      241 ggcctgaacg gcggcaagcc cgcgctcgtg acgcatacaa ccaggtccat gttggcggct
      301 atcgcctgta tggccttgcc cttctcgttg tagcgccaga agaggctctt gcgcggacgg
      361 agcgatagga tcatgccggt gcccgccgag gccggctccc attcgaccac gtctccggcg
      421 gcgagcgggt tatagaagcc ttccgcaccc ttgagcttct tgcccttgag cgcacagtaa
      481 tggacagaac cgtcctcggc caggacggag aacgagttat tggcgccgga cagtaccgta
      541 ccgaggctca aggtaggcac cccgcccacg acaggccgaa gcgctcggcg aagccgccgt
      601 aaaccggtcc ggggtcgccc gcgcctgtga ggaagaggcg gtcggagggc tctccggggc
      661 ggccgctctc ggcgtctcgc atgtcggggc atccaccggt tcgccgcgcg ccgacgctcc
      721 cgcgcaggcc ttcctcgtcg agccgcgacg ccagcctggc ggccacgccg tccctcgagt
      781 cgacgatgga cacgccgggg ccggcgacgg cgccaaactc ctcggacagg tgcagaaaat
      841 gcgtacagcc gaggacgatg acgtcgacgc cgcgctcgag catggcacgc acactgggcg
      901 cgacggcccg caggcgctcg tcgcgggtcg agccgagcag gcggcgttcg acgaagtcga
      961 ccagtccggc gtcaccgatc ctgaccagct cgcagtcggc cgcccaggcc gaggccaggc
     1021 cggtcaggta gtcggccgcg acggcggcct cagtggccac gacgccaatc ctgcggaccc
     1081 gcgacagcgc ggcggccggc ttgatcgcgg gtaccgtgcc gacgacgatc agccctgggg
     1141 tatctgctcg gatagcgtcg acgg
//
LOCUS       DAOOLN010000877         1163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_165001_length_1163_cov_3.320397, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000877 DAOOLN010000000
VERSION     DAOOLN010000877.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165001_length_1163_cov_3.320397"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLW80_10460"
     CDS             complement(<1..242)
                     /locus_tag="PLW80_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66979.1"
                     /translation="MRKPIVLALLVALAMSFCGCQLLFQETAELYVSVDGDYDASVTC
                     ELYLIERLDVGRSAGTGDSILSKKSRTETISGREWMV"
     gene            complement(263..805)
                     /locus_tag="PLW80_10465"
     CDS             complement(263..805)
                     /locus_tag="PLW80_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66980.1"
                     /translation="MKTRAICLALALAMVAGAAHGQAAPAEQPVPLFSDAGLMTGLIL
                     EATGFGLVIGGGATASLSLDAALVMMQIAPICSSVGAWVSQGKMEGYTELWQGRGLEY
                     DASGLIKKSKTAAVATTAFTAGALVLPLLGEVGVYLSLACTGVAVGWDMYAFYAVRSI
                     WLRELNTAIVDSGMDWKAFR"
     gene            902..>1163
                     /locus_tag="PLW80_10470"
     CDS             902..>1163
                     /locus_tag="PLW80_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66981.1"
                     /translation="MDEMSWKAVHECLLEAGCAPTREGLISVFMAGAERLAPCDAGVG
                     VFDRELRCVACVGWDERTRRDYNQYYRGVVPFIGYDAIRYARR"
BASE COUNT          205 a          376 c          375 g          207 t
ORIGIN      
        1 accatccatt cccggcccga tatcgtttcg gtgcgcgact tcttggatag gatgctgtcg
       61 ccggtacccg cgctacggcc cacgtcgagg cgttcgatca ggtacagctc gcaggtgacc
      121 gatgcgtcgt agtcgccgtc gaccgaaacg taaagctcgg cggtctcctg gaacaggagc
      181 tgacagccgc agaacgacat ggccagggcg acaaggagcg ctagaacgat cggtttcctc
      241 atcttcggca ccctcgaccc gctcaacgga aggccttcca gtccatgccc gaatcgacga
      301 tggccgtatt gagctcgcgt agccagatgc tccgcaccgc gtagaaggcg tacatgtccc
      361 agcctaccgc cacgccggtg caggccagcg ataggtagac gcccacctcg ccgagcaacg
      421 gcaacaccag ggcgccggcc gtgaaggcgg tggtcgcgac ggcggccgtc ttggacttct
      481 tgatcaggcc gctcgcgtcg tactcgaggc ctcgtccctg ccatagttcg gtgtagccct
      541 ccatcttgcc ctgcgagacc caggccccta ccgaggagca gatgggcgct atctgcatca
      601 tcacgagggc cgcgtcgagc gatagcgacg cggtggcgcc gccgcctatc acgaggccga
      661 agccggtagc ctcgaggata agtccggtca tcaggccggc gtcggagaag agcggtaccg
      721 gctgttcggc cggcgcggcc tgcccgtggg cggctccggc taccatagcg agcgccaggg
      781 ccaagcatat cgcgcgggtt ttcatggaga cctccaaaca tggatatacc gtaacgctag
      841 cctcccgatt ggtttggcgg tatccgtata ttttacgtat tcatgcgcta tactcgaggc
      901 catggacgaa atgtcatgga aggcggtgca cgagtgcttg ctcgaagccg gttgcgcccc
      961 gacgcgcgag ggcttgatct cggttttcat ggccggggcg gagcggctcg ccccctgcga
     1021 cgccggcgtc ggcgtcttcg atcgggaact gcgctgcgtc gcctgcgtcg gctgggacga
     1081 gcggaccagg cgcgattata accagtacta ccgcggcgtc gtgccgttca tcgggtacga
     1141 cgcgatccgc tacgccaggc gcg
//
LOCUS       DAOOLN010000878         1162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_165319_length_1162_cov_2.250226, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000878 DAOOLN010000000
VERSION     DAOOLN010000878.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165319_length_1162_cov_2.250226"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="PLW80_10475"
     CDS             <1..659
                     /locus_tag="PLW80_10475"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPB66982.1"
                     /translation="TALGGLPRSDPFAERTILTRDDFKQHFIDSGLEEDFAEDEIVRL
                     ERSKRSSNVLSELIVPILFQDYAIGYATVVNRQAGKPPFDLRTVETFLAFARVFSWSL
                     KLHGYFKDAPKLDADYGTQVVDVSAGGLLFACDDLRLIQALKEGTAVSVRLRAKKRTV
                     EASGTIRRHYAGAREGYFGIEFRMMAPEDFRFLFEYLYGRPFTDEDADSVEGIRIVNP
                     "
     gene            complement(739..>1162)
                     /locus_tag="PLW80_10480"
     CDS             complement(739..>1162)
                     /locus_tag="PLW80_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB66983.1"
                     /translation="IVQSAPLEIKEINLLEQQISEVIKNGVKHGNRCEASKKVRVWYS
                     FDSTHAHLIVQDEGEGFKDLEKWNEFNRNRLEILSAQDFEKLSDYVSFRTQKSDDNDG
                     GNALFAALEYWNGGFVFNEARNAVAMLKSFPQKKHAIG"
BASE COUNT          200 a          385 c          351 g          226 t
ORIGIN      
        1 cgacggcgct cggcggcctg ccacgcagcg accctttcgc cgagcggacc atcctgaccc
       61 gcgacgactt caagcagcat tttatcgact cgggcctcga ggaggacttc gcggaggacg
      121 agatcgtcag gctggagcgc tcgaagcgct cgtcgaacgt gctgtccgag ctcatcgtgc
      181 ccatcctgtt ccaggactac gccatcggct acgccacggt agtcaacagg caggcgggca
      241 agccgccgtt cgacctacgc accgtcgaga ccttcctggc cttcgcccgg gtcttctcgt
      301 ggtcgctcaa gctccacggc tacttcaagg acgcgcccaa gctcgacgcc gactacggga
      361 cacaggtggt cgacgtgtcg gccggcgggc tcctgttcgc ctgcgacgac ctgcggctga
      421 tccaggcgct caaggaaggt acggccgttt cggtccgact gagggccaag aagcgcaccg
      481 tcgaggcgag cggcaccata cgcaggcatt acgccggcgc ccgggaaggc tacttcggca
      541 tcgagttccg catgatggcg cccgaggact tcaggttcct attcgagtac ctgtacggcc
      601 gtcccttcac cgacgaggac gccgattcgg tcgagggtat acgcatcgtc aacccgtagc
      661 aggccacgaa gacccctcgc cataggaaga ggcggcccgc gagggccgcc tttccgtcgt
      721 cacggtggga aaccgggctc agcctatggc gtgcttcttt tgcgggaagc tcttgagcat
      781 cgctacggcg ttcctcgcct cgttgaacac gaagccgccg ttccagtact cgagggcggc
      841 gaacagcgcg ttgccgccgt cgttgtcatc gctcttctgc gtcctgaacg atacgtagtc
      901 cgacagcttc tcgaaatcct gggcggacag gatctcgagg cgatttcggt tgaactcgtt
      961 ccacttctcc aaatccttga agccctcgcc ctcgtcctgg acgatcaggt gggcgtgggt
     1021 cgagtcgaag gagtaccaga ccctcacctt cttcgaggcc tcgcaacgat tgccgtgctt
     1081 gacgccgttc ttgatcacct cgctgatctg ctgctcgagc aggtttatct ccttgatctc
     1141 gagcggggcc gactgcacga tc
//
LOCUS       DAOOLN010000879         1162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_165335_length_1162_cov_2.085818, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000879 DAOOLN010000000
VERSION     DAOOLN010000879.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165335_length_1162_cov_2.085818"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..>1162)
                     /locus_tag="PLW80_10485"
     CDS             complement(196..>1162)
                     /locus_tag="PLW80_10485"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB66984.1"
                     /translation="KLSRPAILDEIQRVPELLSYIQVMVDDEGLNGSFILTGSHQLRL
                     GQAVSQSLAGRTAVLTLLPLSIEELSAAVRDDERGSMLRRGFLPRIHDQSQEPTRAYR
                     NYFQTYVERDLRSLLLVKDLSKFETFMRLLAGRTGRLFVASSLATELGVSYKTVQEWV
                     SILEASYIVRLLRPYHANYGKRLVKTPKLYFVEPGLACYLLGIESDDQVERDPLFGSL
                     FENMVVMEAVKARANRGLEPSLYFIRDSNGVEVDLVLDRRPVPLPIEIKASTTYHPDF
                     RKGLERFDAMIGTSATGYVVYGGDADQVGDSLRLIGYKRLSTILT"
BASE COUNT          249 a          327 c          357 g          229 t
ORIGIN      
        1 ggatgcggaa gaactatacc gagcgataac ggagaagatg tcgtatagag ggaacgcgga
       61 acatgatggt acaggattga agaagatgaa caagattaag caaagaccag gtttaggact
      121 tatcctgatc atcctgctac atcttgatca tcatgttctg cattcttagc gatcttcacg
      181 tggggatata ggcccctagg tcagtatcgt cgataggcgc ttgtagccga tgagcctgag
      241 cgaatcgccg acctgatccg cgtcgcctcc gtataccacg tagccggtgg cgctcgtccc
      301 tatcatggcg tcgaagcgtt ccagcccctt gcgaaaatcc gggtggtagg tcgtggaagc
      361 cttgatctcg ataggaagcg gtacgggcct acggtccagg accaggtcga cctcgacgcc
      421 gttactgtcg cgtatgaaat agagcgacgg ctcgaggccg cgattggccc gggccttgac
      481 cgcttccatg acgaccatgt tctcgaatag gctgccgaat aggggatccc gctccacctg
      541 atcgtcgctc tctattccca agaggtaaca agccaacccg ggctcgacga aatagagctt
      601 cggcgtcttg acgagcctct tcccgtaatt agcgtgatag ggtcggagca ggcggacgat
      661 gtacgaggcc tcgagtatcg acacccactc ctggacggtc ttataggata cccctagctc
      721 ggtagccaag gacgaggcga cgaacagccg gcccgtacgg ccggccagga gcctcatgaa
      781 ggtctcgaat tttgacaggt ccttcacgag gagcagggat cgcaggtccc gctcaacgta
      841 ggtctggaag tagttccgat acgccctggt aggttcctgc gactggtcgt ggatacgcgg
      901 taggaagccc cggcgcagca tcgagccgcg ctcgtcgtcc cgcaccgcgg cggagagctc
      961 ctcgatgctc aggggcagga gcgtcagtac cgcggtccta cccgcaaggg actgggagac
     1021 ggcctgcccg agcctgagct gatggctgcc ggttaggatg aacgagccgt tcagtccctc
     1081 gtcgtccacc atgacctgga tatacgaaag cagctcgggg acgcgctgta tctcgtctag
     1141 gatcgccggg cgcgacagtt tg
//
LOCUS       DAOOLN010000880         1159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_165963_length_1159_cov_2.547101, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000880 DAOOLN010000000
VERSION     DAOOLN010000880.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165963_length_1159_cov_2.547101"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PLW80_10490"
     CDS             <1..796
                     /locus_tag="PLW80_10490"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HPB66985.1"
                     /translation="VLGKRLKDKPLRVEENLDALEIRLSAWVDRYLKDSRDEMKETYS
                     YAGALNLDSFFDIMKYSPGTEEYAVLWAIEHIHCELSPDYDLVVYDTPPTALSLRFLA
                     MPAISNMWITELAKLRERILQRRQIVTKINPESPVASSCVDKDDDKVYGRLGSIKRRL
                     ALLQKLFSEESFVAVVINPDSLSVSEALRIKEELERIDIPFSAICVNKRGLSKAPWQL
                     DAELAEIPAFYFDFKPEGLHERDDLMRLDTQDLVAAFDAAASEGIR"
     gene            793..>1159
                     /locus_tag="PLW80_10495"
     CDS             793..>1159
                     /locus_tag="PLW80_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66986.1"
                     /translation="MNPYALVALVVLAIAATAQYYLGTRKNRFLTSRMSGALEELLKP
                     STTNYVNIGGSIGYNFAYSLSGDWTKAKGTMTLNARHSLLYLPFSLLLGVRDRFYVNI
                     FTKRKLRGEAHLVEAKYLKH"
BASE COUNT          233 a          367 c          342 g          217 t
ORIGIN      
        1 cgtgctgggg aagaggctca aggacaagcc gctcagggtc gaagagaacc tcgacgcgct
       61 cgagatccgg ctgtccgctt gggtggatcg ctacctcaag gatagccgtg atgagatgaa
      121 ggagacctac tcctacgcgg gcgccctgaa cctcgactcg ttcttcgata tcatgaaata
      181 ctccccgggc accgaggagt acgcggtgct ctgggccatc gagcacatcc attgcgagct
      241 atcgccggac tacgacctcg tcgtctacga cacgcctccg acggctcttt ccctgcggtt
      301 cctggccatg ccggccatat cgaatatgtg gatcaccgag ctggccaagc tacgcgagcg
      361 tatcctgcag cgtcggcaga tcgttaccaa gatcaatccc gagtcgccgg tcgcctcgag
      421 ctgcgtcgac aaggacgacg acaaggtcta cggccggctc ggctcgatca agcgtcgcct
      481 ggcgctgctc cagaagctgt tcagcgagga gagcttcgtc gcggtcgtca ttaacccgga
      541 ctcgctgtcg gtatcggaag cgctgcgcat caaggaagag ctcgagcgca tcgatatacc
      601 gttctccgcc atctgcgtca ataagcgcgg cctgtccaag gcgccctggc agctcgacgc
      661 cgagctggcg gagatcccgg cgttctactt cgacttcaag ccggaaggct tgcacgagcg
      721 tgacgacctc atgaggctcg acacccaaga tttggtggcc gctttcgacg cggccgcctc
      781 ggaaggcatc agatgaatcc atacgcactc gtggctctcg tcgtgctggc catagcggcg
      841 acggcccaat actacctggg taccaggaag aaccgcttcc tgacctcgcg catgtccgga
      901 gcccttgagg agctgctcaa gccgagcacg accaattacg tgaacatcgg cggctccatc
      961 ggctacaatt tcgcgtactc gctgtcgggc gactggacca aggcgaaggg caccatgacc
     1021 ctcaacgctc gccattcgct cctgtacctg ccgttctcgc tgctgctcgg ggtgcgcgac
     1081 cgattctacg tgaacatttt cacgaagagg aagctgcgcg gagaggcgca cctagtagag
     1141 gctaagtacc tgaagcacg
//
LOCUS       DAOOLN010000881         1159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_165981_length_1159_cov_2.381341, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000881 DAOOLN010000000
VERSION     DAOOLN010000881.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165981_length_1159_cov_2.381341"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PLW80_10500"
     CDS             complement(<1..553)
                     /locus_tag="PLW80_10500"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HPB66987.1"
                     /translation="MLIVPYGLARVELTVVNSRFIASLAPADSVEAARAYIAGIRAEF
                     PDATHNVPAFIIGGGNAVTEFCSDDGEPSGTSGRPLLAALKGSGLGNAVVVVTRYFGG
                     TLLGTGGLVKAYSEAGKAVLAVARRAELVETRRLVFEAPYHLFERVRSLAEASRATIV
                     AEDFSESVALELDVAAADCDAFLS"
     gene            643..>1159
                     /locus_tag="PLW80_10505"
     CDS             643..>1159
                     /locus_tag="PLW80_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66988.1"
                     /translation="MAPSRAGLLWIFIRPYVDSDVRLFKKAKLLAPVLVAIAALAVIL
                     ATLMAVTGAVIVAAALTALIGLCVVSLVLLARGRYGASSTVFIYSLFAVMFGAIKFDA
                     YQNVYETYVFGTLGGFMLVITGLIAAKPIQAWIMTLLDLLAIGALYYLDALPLDGGVV
                     TELAMQNLGTCG"
BASE COUNT          200 a          400 c          351 g          208 t
ORIGIN      
        1 ccgataggaa cgcgtcgcag tctgccgcgg ccacatcgag ctccagggcg acgctctcgg
       61 agaaatcctc cgctacgatg gtcgcgcggc tcgcctcggc gagcgaacgt acgcgctcga
      121 acaggtggta cggcgcctcg aagacgaggc gacgagtctc cacgagctcg gcccgccgtg
      181 cgaccgcgag cacggccttg ccagcctccg agtaggcctt gaccaggccg ccggtcccga
      241 gcagcgttcc gccgaaatat ctcgtgacca cgacgacggc gttgccgagc cccgagccct
      301 tgagcgcggc cagaagtggc cggcccgagg taccggacgg ctcgccgtcg tcggagcaga
      361 actcggtaac ggcattgccg cccccgatga tgaaggccgg cacgttgtgg gtcgcgtccg
      421 ggaattcggc acgtataccg gcgatatagg cgcgggcggc ctcgaccgaa tcggccggag
      481 ccaagctggc tatgaagcgc gagttgacca cggtcagctc gaccctggcc aagccgtacg
      541 gaacgatcag catgccccac tatagcacgg ctagccctcg gaaagcttgt ctcatccgga
      601 tcatacgcta tactataggt agctttaaac tatgaggtgt atatggctcc atcgcgcgcc
      661 ggacttttat ggattttcat ccgtccgtac gtcgactcgg acgtgcggct cttcaagaaa
      721 gccaagctcc tggctccggt cctcgtagcc atagccgcgc tcgcggtcat cctagcgacc
      781 ctgatggcgg taaccggagc tgtcatcgta gcggccgccc tgacggccct gatcggcctc
      841 tgcgtcgtgt cgctggtact cctggccagg ggccgctacg gcgcctcgtc gaccgtcttc
      901 atctactccc tgttcgccgt catgttcggc gccatcaagt tcgacgccta ccagaacgtc
      961 tacgagacct acgtcttcgg cacgctcggc ggcttcatgc tcgtaatcac cggcctgatc
     1021 gccgccaagc cgatccaggc ctggatcatg accctgctcg acctgctcgc gatcggcgcc
     1081 ctttactacc tcgacgcgct gcccctcgac ggaggcgtgg tcaccgagct ggcgatgcag
     1141 aacctcggca cctgcggcg
//
LOCUS       DAOOLN010000882         1158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_166279_length_1158_cov_1.807797, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000882 DAOOLN010000000
VERSION     DAOOLN010000882.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166279_length_1158_cov_1.807797"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..>1158
                     /locus_tag="PLW80_10510"
     CDS             76..>1158
                     /locus_tag="PLW80_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66989.1"
                     /translation="MAQSLMDRFLVRYAEAEPLDRKQTSALVYTLVSIIAAACLAAVM
                     VDNSGIRLVLVCLTSILVGILVLVRVGQAVISSVATTILVSLLFASIAFMQPYEEAYE
                     IYLIATLECFALIMTGLVARRRWQAIAVMVVALAAVSLDYFTRIIPNQVEPGENLNDF
                     VISTIILIIATAIERAIRVRDYSLLAISRTEAAKSKAYIDRLETLIRSSGDTLGLGIA
                     ARKSAESTASLVASMSATLDGADAGFSELEASSAAILSSYARIVDSARLVESKVADQS
                     AVITESSAAIEQMTSAVNSISTIAAARREAVGKLKSATDDGSSQMSDLADALRAMEAA
                     AASIVEVVEVIRAVASQTNLLAMNAAI"
BASE COUNT          207 a          387 c          345 g          219 t
ORIGIN      
        1 gtcgactagg gacagaacgg cgtagcgagt aaataatcct ggcctatccg tcgatactcg
       61 atactaggag aaataatggc acagtccctg atggataggt tcctggtccg ttacgccgaa
      121 gcggaacccc tggaccgcaa gcagacctcg gcgctcgtct atacgctggt ctctataatc
      181 gccgccgcct gtttggcggc ggtcatggtg gataattctg gcatcaggct ggtcctcgta
      241 tgtctcacgt ctatcctcgt cgggatactc gtattggttc gggtagggca ggccgtcatc
      301 tcgagcgtgg ctacgaccat tctcgtgtcc ctgctcttcg cctccatagc cttcatgcag
      361 ccctacgagg aagcctacga gatctacctc atagccacgc tcgagtgctt cgcgctgatc
      421 atgaccgggc tcgtcgcgcg caggcgctgg caggcgatag ctgtgatggt cgtcgcgttg
      481 gccgccgtct cgctcgacta tttcacgcgg atcatcccca accaggtcga gcccggcgag
      541 aacctgaacg acttcgtcat ctcgacgatc atcctgatca tcgccacggc gatcgagcgg
      601 gctatcaggg tccgcgacta cagcctgttg gcgatcagca ggaccgaggc cgccaagagc
      661 aaggcctata tcgataggct tgagaccctg atccgctcga gcggcgacac cctgggcctc
      721 ggcatcgccg ctcgcaagtc tgccgagtcc accgcctctc tcgtcgcctc gatgagcgcc
      781 acgctcgacg gcgctgacgc gggcttctcg gagctcgagg ccagctcggc cgccatactc
      841 tcgtcgtatg cgcgcatcgt cgactccgcc cgcctggtcg agtcgaaggt ggccgaccag
      901 tcggccgtca tcaccgagtc gtccgcggcg atcgagcaga tgaccagcgc cgtcaatagc
      961 atctccacca tcgccgcggc gagacgcgag gctgtcggca agctcaagag cgcgaccgac
     1021 gacggcagct cgcagatgtc cgacctggcc gacgcgctgc gcgccatgga agccgccgcc
     1081 gcgtccatcg tcgaggtggt cgaggtgatc cgcgccgtcg ccagccagac gaatctgctc
     1141 gcgatgaacg ccgccatc
//
LOCUS       DAOOLN010000883         1157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_166359_length_1157_cov_3.653358, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000883 DAOOLN010000000
VERSION     DAOOLN010000883.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166359_length_1157_cov_3.653358"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PLW80_10515"
     CDS             complement(<1..601)
                     /locus_tag="PLW80_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66990.1"
                     /translation="MRLKSSTISLCRKILYESLTTDAMVRFVRLVEPEYDLHERSGIP
                     VTIPITNQMAAERVVSDILADGRFIDFVEVLVKIDAEGYMGRSYPIKGLGQIVKILEQ
                     DGFVLDRSTGQFFENTNERASPDWGRLHEGDERQTSILRLDVVDNSALVQRNRPECVD
                     RAFVALRSIVSRAVVSRFGRVWSWEGDGALAAFMLGRKER"
     gene            complement(671..>1157)
                     /locus_tag="PLW80_10520"
     CDS             complement(671..>1157)
                     /locus_tag="PLW80_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HPB66991.1"
                     /translation="IDFVGVNYYNEDAVVAAPPTAKRPDGYDMAPTARERTEMGWDVA
                     PQGLRRVLGFIADRWPVRALYVTENGAAYPDAAIRDGRAHDADRIAYYRGHIAACADA
                     IGDGVPLRGYFAWSLLDNFEWSHGYGKRFGLVSVDPMTMARRPKDSYYFYRDVIAGFL
                     E"
BASE COUNT          212 a          367 c          373 g          205 t
ORIGIN      
        1 atcgttcctt ccggcctaac atgaaggcgg cgagcgctcc gtctccctcc caggaccata
       61 cgcgcccgaa tcgcgagacg acggcgcggc tgacgatgga gcgtagcgcg acgaaggccc
      121 tatcgacgca ttccggccgg ttgcgctgca ccagggccga attgtcgaca acgtcgagcc
      181 tgagtatcga ggtctgccgt tcgtcgccct cgtggagtcg gccccaatcg gggctggcgc
      241 gctcattggt gttctcgaaa aactgccccg tggaccggtc cagcacgaag ccgtcctgtt
      301 cgaggatctt gacgatctgc cccaggccct tgatcgggta cgatcgcccc atatagccct
      361 cggcgtcgat cttgacgagg acctcgacga aatcgatgaa ccggccatcg gcgaggatat
      421 cggagacgac ccgctccgcc gccatctggt tcgtgatggg gatggtgacc ggaatgcccg
      481 agcgttcatg caggtcgtat tcgggctcca cgagcctgac gaaacggacc atggcgtccg
      541 tcgtcagcga ttcgtagagt atcttcctgc agaggctaat ggtagagctc ttcaatctca
      601 ccgctatgcc accaacgtta tcggatgacg atagtatagc acacgccggc tggcgatgcg
      661 aacgagagcg ctactccagg aaaccggcta tgacgtcacg gtagaaataa tagctatcct
      721 tgggtcgccg tgccatcgtc atcgggtcca ctgacacgag accgaagcgc ttaccgtagc
      781 cgtgcgacca ctcgaagtta tcgagcaggc tccaggcgaa gtaaccccgg agcggcacgc
      841 cgtcgccgat ggcgtcggcg caggcggcga tatggccgcg gtaataggcg atacggtcgg
      901 cgtcatgcgc gcggccgtcc cggatcgccg cgtccgggta ggcggcgcca ttctcggtca
      961 cgtagagggc gcgcacgggc cagcgatcgg ctatgaagcc gagcacgcgc ctgagcccct
     1021 ggggcgccac gtcccagccc atctcggtcc gctcgcgggc cgtcggcgcc atatcgtagc
     1081 cgtcggggcg cttggccgtg ggcggggcgg cgacgacggc gtcctcgttg tagtagttga
     1141 cgccgacgaa atctatg
//
LOCUS       DAOOLN010000884         1157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_166389_length_1157_cov_3.056261, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000884 DAOOLN010000000
VERSION     DAOOLN010000884.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166389_length_1157_cov_3.056261"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..>1157
                     /locus_tag="PLW80_10525"
     CDS             167..>1157
                     /locus_tag="PLW80_10525"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPB66992.1"
                     /translation="MMKDIRRALMGAVLLCCSVGPLVAQTALPGRPDPVASLPDGLYA
                     VIETVRGTVVLELYADKAPFAVASFVGLAEGSFSQDGRPYYDGVSFHRVEPGFVVQGG
                     DPTGTGRGGPGYQFPNEITPELGFNAPGVLGMANAGPDTNGSQFFITLGAANHLNGGY
                     TVFGRVVVGMDAVRSIRKGDAMTRVTVARKGAKARAFKADKARFDADLRAYASGAAER
                     AKAGVEGQFAAVKARIPGLALGADGLWYKALAAGNGAKPATGSMVRILYTLTLADGRR
                     LDSTADRGNEPLEFRLGGGQVIPGFDKAVSAMSYGERRVVVIPPELGYGSRGAG"
BASE COUNT          166 a          395 c          412 g          184 t
ORIGIN      
        1 cgagacgccc ggtacgccgg cggcggcccg ttcgctcgcc ctggcgcagg cggagcagct
       61 catccccttg atctcgacgg tgtcggtctt catcgatata ccccttgggg gtataatacc
      121 aacttgccgt tcgatcgtcc atagcgtatc ctcgccgcgg aggatcatga tgaaagatat
      181 ccgtcgggcc ttgatgggcg cggttctatt gtgctgttcc gtcgggccgc tcgtcgccca
      241 aacggccctg ccaggtcggc cggatccggt cgcctcgttg ccggacgggc tgtacgccgt
      301 gatcgagacg gtccgcggta ccgtcgtact cgagctgtac gccgacaagg cgcccttcgc
      361 ggtcgccagc ttcgtcggcc tcgccgaggg ctcgttctcg caggacgggc ggccgtacta
      421 cgacggggtc agcttccatc gcgtggagcc gggcttcgtg gtccagggcg gcgacccgac
      481 gggtaccggc cgcggcggcc cgggctacca gttcccgaac gagataaccc cggagctcgg
      541 cttcaacgcc ccgggcgtgc tcggcatggc caacgcgggg ccggatacga acggtagcca
      601 gttcttcata acgctcggcg ccgcgaacca cctgaacggc gggtacacgg tcttcgggcg
      661 cgtggtcgtc ggtatggacg cggtccgctc catccgcaag ggcgacgcga tgaccagggt
      721 gaccgtcgcg cgcaagggag ccaaagcccg ggcgttcaag gcggacaagg cccgcttcga
      781 cgccgacctg cgcgcctacg cgtccggggc cgccgaacgg gccaaggctg gcgtcgaggg
      841 gcagttcgct gcggtcaagg cgcggatacc cggcctggcg ctcggcgccg acgggctgtg
      901 gtacaaggcg ctcgccgccg ggaacggcgc caagccggcc accggctcga tggtgcgcat
      961 cctgtatacc ctcacgctgg ctgacggccg ccggctcgac tcgacggccg accgcggcaa
     1021 cgagccgctc gagttcaggc tgggcggcgg ccaggtgata cccggcttcg acaaggcggt
     1081 gtccgctatg agctacggcg agcgccgcgt cgtcgtcata ccgccggagc tgggttacgg
     1141 ctcgcgcggg gctggcg
//
LOCUS       DAOOLN010000885         1156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_166625_length_1156_cov_3.232516, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000885 DAOOLN010000000
VERSION     DAOOLN010000885.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166625_length_1156_cov_3.232516"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..252)
                     /locus_tag="PLW80_10530"
     CDS             complement(103..252)
                     /locus_tag="PLW80_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66993.1"
                     /translation="MGQLIGALERTILYVLIVSGNFTEYFLVGTMASVCVTLVLGLLV
                     RLALA"
     gene            complement(240..752)
                     /locus_tag="PLW80_10535"
     CDS             complement(240..752)
                     /locus_tag="PLW80_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66994.1"
                     /translation="MDGLAFHAARDALEGLKAEKRMILDVSWPGSPWVALVNASLRSF
                     AHGMEGWNATAVCAFAGLMNGLSVDEVAKEAGVTRRMVYKAMATRGLRDEAALFTAVT
                     AGLGRLEKEPPGRVGPGYRPGRYGAACSSPWAWSCAPSRRTTWSWRRSNASQPFPKTE
                     PGTIGRAWAS"
     gene            complement(843..1049)
                     /locus_tag="PLW80_10540"
     CDS             complement(843..1049)
                     /locus_tag="PLW80_10540"
                     /inference="COORDINATES: protein motif:HMM:NF027589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HPB66995.1"
                     /translation="MKALCVIADLVDSRGIADRGRFQRGLKASLEALSSRRGARLLSP
                     YTITLGDEFQALYRDSGTLLADFA"
BASE COUNT          220 a          385 c          362 g          189 t
ORIGIN      
        1 tcttcgagtc ggtccgcgcc gtgtccgccg agaccgtcga ctacggctcc ggcgtcctgc
       61 cctggatgct agccggcggg gtcagcgtgt cggggaagta ggctacgcca gggccagccg
      121 tacgagcagg cccagcacca gcgtgacgca gaccgaggcc atggtaccta ccaggaagta
      181 ctcggtgaag ttgcccgaga cgatgagcac gtacaggatg gtccgctcga gcgcgccgat
      241 cagctggccc atgcccggcc tatcgtccct ggctccgttt ttgggaaagg ctgagaagcg
      301 tttgagcgcc gccacgacca ggtagttcgc ctcgacggcg cacagctcca ggcccacggc
      361 gacgagcagg ccgcgccgta gcgccccggc cgatagccag ggccaacccg gccaggcggc
      421 tccttctcca ggcgcccgag gccggccgta accgccgtga agagcgcggc ctcgtcgcgg
      481 agccccctcg tcgccatggc cttgtagacc atgcgccgcg tcacgcccgc ctccttcgcg
      541 acctcatcca cggacagtcc gttcatgaga cctgcgaacg cgcagacggc ggtagcgttc
      601 cagccctcca taccgtgggc gaaggaacgg agcgacgcgt tgacgagcgc tacccacggc
      661 gagcccggcc agctcacgtc caggatcata cgtttctcgg cctttaggcc ctcgagcgcg
      721 tcgcgcgccg cgtggaacgc cagcccgtcc atcccgaggg cctcacgaac gttgatgtcg
      781 gtgcttatat cgtcataggc ggctgatagg cgcgcccgtt gcgagaagat ggcccaggcg
      841 agtcaggcga aatcggcgag cagggtgccg gaatcgcgat acaaagcctg gaactcgtcc
      901 cccagcgtga tcgtatatgg cgagagaagc ctggctcccc gccgcgagct cagggcctcg
      961 agactcgcct taaggcctcg ttggaagcgc ccgcggtcgg cgatgcccct cgagtcgact
     1021 aaatccgcga tgacacagag agccttcacg gaatcaagag taccactatg gttcatcagc
     1081 tgtcatgtgt taattttagt tcgtttttaa agagagaact aaataagcac agagataccc
     1141 actcctcgca ggtgaa
//
LOCUS       DAOOLN010000886         1156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_166691_length_1156_cov_2.415985, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000886 DAOOLN010000000
VERSION     DAOOLN010000886.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166691_length_1156_cov_2.415985"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PLW80_10545"
     CDS             <1..540
                     /locus_tag="PLW80_10545"
                     /inference="COORDINATES: protein motif:HMM:NF024674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HPB66996.1"
                     /translation="VPGRGFRAYQTPKGPVVVAQLLGQAGFPRVHLENPFHVLDAALP
                     TLSEGSKAVLLDFRAATTAERRAMARYADGRVSAVLGSYGRALSADAEVSAAGTASMT
                     DTGRTGSLMSVGGMDAATRIHEYRSGVPVWAKDSSEAPELQGCVIELGADGRAVSIET
                     LRVPCEEEFVEGTRHSDQG"
     gene            527..916
                     /locus_tag="PLW80_10550"
     CDS             527..916
                     /locus_tag="PLW80_10550"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HPB66997.1"
                     /translation="MTKVDGSDVTVRISGGEGCASCTAGHDCSIVGKELVGDAAAGYA
                     FAAGDEVELLVPDSASAAGALWLLAVPLGLFFAGYAAAGALWPGRGEGLRALLGLGAM
                     ALGLLFAAVVARRGRMSRRPTVVPLVA"
     gene            complement(913..>1156)
                     /locus_tag="PLW80_10555"
     CDS             complement(913..>1156)
                     /locus_tag="PLW80_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HPB66998.1"
                     /translation="APALVLGEACGLLAGRRFTCYPGLEGRVPEGRFEPSRVVVDREL
                     ITARGPGCAGEFAVAIARALVGDEKADELADGVLLS"
BASE COUNT          152 a          396 c          434 g          174 t
ORIGIN      
        1 gtgcctggca ggggcttccg ggcctaccag acgcccaagg gcccggtcgt cgtcgcgcag
       61 ctgctcggcc aggcgggctt cccgcgggta cacctcgaga acccgttcca tgtgctcgac
      121 gccgctctgc cgaccctgtc ggagggatcg aaggcggtac tgctcgactt ccgcgccgcc
      181 acgacggcgg agcgccgggc aatggccagg tacgcggacg gcagggtcag cgccgtgctc
      241 ggctcctatg gccgcgcgtt gagcgccgac gccgaggtgt cggcagccgg taccgccagc
      301 atgaccgata ccggccggac cggcagcctg atgtcagtcg ggggcatgga cgccgctacg
      361 aggattcatg agtaccggag cggggtaccc gtctgggcca aggactcgtc ggaggcaccc
      421 gagcttcagg gctgcgtcat cgagctagga gcggacgggc gcgccgtgtc gatcgagacg
      481 ctacgcgtgc cgtgcgagga ggaattcgtt gagggaacga ggcatagtga ccaaggttga
      541 cggtagcgac gtcaccgtcc gtatttccgg cggagagggc tgcgcgtcct gcaccgccgg
      601 ccatgattgc tcgatcgtcg gcaaggaact ggtcggcgac gccgccgcgg gttacgcctt
      661 cgccgcgggc gacgaggtgg agctgctggt gccggattcg gccagcgcgg ccggggcgct
      721 ctggctgttg gcggtgccgc tcgggctgtt cttcgccggc tacgccgcgg cgggcgcgct
      781 gtggcctggc cgcggcgagg ggctccgagc gctgctcggg ctcggggcga tggccctcgg
      841 cctcctgttc gccgccgtcg tggcgcgccg cggccgcatg tccaggcggc cgacggtcgt
      901 ccccctcgtc gcctaggaca aaagtacgcc gtcggccagc tcgtcggcct tttcgtcgcc
      961 gacgagggcc ctggctatcg ctacggcgaa ttccccggcg cagcccggtc ccctggccgt
     1021 tatgagctcc cggtcgacga cgacgcggct tggctcgaac cggccctcgg ggacccggcc
     1081 ttcgagcccg ggatagcagg taaaacggcg cccggccagt agcccgcagg cctcgccgag
     1141 caccagggcc ggggcg
//
LOCUS       DAOOLN010000887         1154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_167057_length_1154_cov_4.427662, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000887 DAOOLN010000000
VERSION     DAOOLN010000887.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167057_length_1154_cov_4.427662"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..737)
                     /locus_tag="PLW80_10560"
     CDS             complement(54..737)
                     /locus_tag="PLW80_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB66999.1"
                     /translation="MHDHALSLLDFYRIRDDVAGYCLSEEGAAAVRAGLPSADPDEVV
                     DAKARASAVRELLRDSRDVPGGSFMPVDAASRIIGKQGSCLAVEELWALGMWAAAYAG
                     LVRWLSGVRHEGLRAELTAAPDLSAVAAAAFRVVGQDGEIRDLPELRDIRSKIRAINL
                     DIERVTASFFQDESTRQMLQNDVPTQRDGRTVLAVKAGSRSRLKGIVHEVSSTGQTVY
                     IEPETLVQQ"
     gene            complement(730..>1154)
                     /gene="rsgA"
                     /locus_tag="PLW80_10565"
     CDS             complement(730..>1154)
                     /gene="rsgA"
                     /locus_tag="PLW80_10565"
                     /inference="COORDINATES: protein motif:HMM:NF015170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase RsgA"
                     /protein_id="HPB67000.1"
                     /translation="SLLNAIESGLGLKVGEVCETYERGRHTTVAAILPVLSDGVTRVI
                     DTPGFRRLAVRGIDPVTLGACFPEIRAASAGCTLGSRCKHLDEPGCAVVAALESGGVH
                     EDRYESYARILAELEDTRAYAKKAGRPRRHHEDDYEDA"
BASE COUNT          179 a          385 c          395 g          195 t
ORIGIN      
        1 ggagcacgcg ctggagttca cgcaggtagc gcgcctcctc ctcgaccagc tcgttattgc
       61 tggacgaggg tctccggctc gatatagacg gtctgcccgg tggacgacac ctcgtgtacg
      121 atgcccttga gcctcgagcg ggaacccgcc ttgaccgcta gcacggtccg accgtcgcgc
      181 tgggtcggta cgtcgttctg gagcatctgt cgggtcgact cgtcctggaa gaaggaggcc
      241 gtgacgcgct ctatgtcgag gttgatggct cgtatcttcg agcggatatc gcgaagctcg
      301 ggcaggtcgc gtatctcgcc gtcctgcccg acgaccctga acgcggcggc cgctaccgcc
      361 gacaggtcgg gcgcggccgt gagctcggcg cgtaaccctt cgtgcctgac gccggacagc
      421 caacgcacga gcccggcgta ggcggcggcc cacatgccga gcgcccagag ttcctccacg
      481 gccaggcacg agccctgctt gccgatgatg cgcgaggcgg cgtcgacggg catgaacgat
      541 ccgccgggaa cgtcccgcga gtcgcggagc agctctcgga ccgccgaggc ccgagcctta
      601 gcgtccacca cctcgtccgg gtcggccgag ggcaagcccg ccctgacggc ggcagcgcct
      661 tcctcgctca ggcaatatcc ggcgacgtcg tcgcggatac ggtagaaatc gagtagcgat
      721 agcgcgtggt catgcatctt cgtaatcgtc ctcatggtgt cgtcgcggcc tgccggcttt
      781 cttggcataa gcacgcgtat cttcgagctc ggccaggatg cgcgcgtagc tctcgtagcg
      841 gtcctcgtgg acgccgcccg actcgagggc ggccacgacc gcgcaaccgg gctcatcgag
      901 gtgcttgcat cggctaccga gggtgcagcc cgcggaggcg gctcgtatct ccgggaagca
      961 ggcgccgagg gtcacggggt ctatgcccct gaccgccagg cgcctgaaac cgggcgtgtc
     1021 gatgacgcgg gtgacgccgt cggatagcac cggtagtatc gcggcgacgg tcgtatgccg
     1081 accgcgctcg tatgtctcgc agacctcgcc gaccttgagc ccgaggcccg attcgatggc
     1141 gtttaagagg gagc
//
LOCUS       DAOOLN010000888         1154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_167158_length_1154_cov_2.274795, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000888 DAOOLN010000000
VERSION     DAOOLN010000888.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167158_length_1154_cov_2.274795"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="PLW80_10570"
     CDS             complement(<1..1108)
                     /locus_tag="PLW80_10570"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67001.1"
                     /translation="MSERQGARQTKASGGRGAGVISARSFVASLAVLAALMLAAGVLT
                     RLVPAGSYERSAGADGALVVVPGSYVETARPDYPAWRWLTAPFEVLASEDAALAIVII
                     AFIAVVGGAFAAMREAGVLAESVRLLAERFKARRMALLAVLCLFFMAIGSFMGVFEEV
                     VPLVPIAIALSLSFGWDVFAGLGMSILAVGFGFSSAVANPFSVGTAQRLAGVPMLSGS
                     GFRLVVFACVYALYLTFMVGYVRRLERKGLGSTMDRADGSPVSASAYGSGSAGSPGSA
                     GDTGRAGPDQGATSPAAPFVSRGVRFFGWSALVLAILVLLSSVTRVGADYVLPAIALG
                     FLVIGVGAALAAGVRLGAVARSFGTGALGVLPAGL"
BASE COUNT          215 a          407 c          423 g          109 t
ORIGIN      
        1 ggagcccggc cggcagcacg ccgagggcgc cggtcccgaa cgagcgcgcc accgcgccga
       61 gccttacgcc ggcggccagg gcggcgccga ccccgatgac caggaagccg agcgctatcg
      121 ccggcagcac gtagtcggcg ccgacgcggg taacggacga caggagcacg aggatagcga
      181 ggacgagggc agaccagccg aagaagcgca ccccgcgcga gacgaagggg gcggccggag
      241 aggtagcgcc ttggtccggg ccggctcggc cggtatcccc ggccgagccg ggcgaaccgg
      301 cggagccgga gccgtaggcg gaggcggaga ccggcgagcc gtcagcacgg tccatcgtcg
      361 accctaggcc tttccgctcg agtcgcctca cgtagccgac catgaaggtc aggtacagcg
      421 cgtagacgca ggcgaagacg acgagcctga agcccgatcc cgacagcatc ggtaccccgg
      481 cgaggcgttg ggcggtgccg acggagaagg gattggccac ggccgacgag aagccgaagc
      541 cgaccgccag gatgctcatg ccgaggcccg cgaagacgtc ccagccgaag gacagggaca
      601 gggctatcgc gatgggcacg aggggcacga cctcctcgaa gacgcccatg aagctgccta
      661 tggccatgaa gaacaggcac aggacggcca ggagggccat ccgcctggcc ttgaagcgct
      721 ccgcgaggag gcggacgctc tcggcgagca cgccggcctc tcgcatggcg gcgaaggcgc
      781 cgccgaccac cgcgatgaac gcgatgatca cgatcgccag ggccgcgtcc tcggaggcga
      841 gcacctcgaa gggggccgtg agccagcgcc aggccgggta gtccggccta gccgtctcga
      901 cgtagctgcc cggcaccacg acgagggcgc cgtcggcccc ggccgagcgt tcgtacgaac
      961 cggccggcac gagccgcgtc aggacaccgg cagccagcat cagcgccgcg agcaccgcga
     1021 gcgacgcgac gaacgagcgt gccgatatga ccccggcgcc ccggccgccc gaagccttcg
     1081 tctgtctagc gccttgtcgt tcactcatgc tcgcgcctcc gcgatccagg gtacacggcc
     1141 ctggcggagg ccgc
//
LOCUS       DAOOLN010000889         1153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_167286_length_1153_cov_3.856102, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000889 DAOOLN010000000
VERSION     DAOOLN010000889.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167286_length_1153_cov_3.856102"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1153)
                     /locus_tag="PLW80_10575"
     CDS             complement(<1..>1153)
                     /locus_tag="PLW80_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPB67002.1"
                     /translation="MESERPMDRLVCGDVGYGKTEIAMRAAFKAVMGGKQVAFLAPTT
                     ILAEQHYENCQDRFRGLPVRFGMLSRFVDRKEQKKTLAGVAEGSIDVLVGTHRILQKD
                     VAFKDLGLLIVDEEQRFGVKDKERLKELRANVDCLTLTATPIPRTLHMSMLKIRDLSV
                     LTTPPNNRHPIQTVVDEFQPDLIAKAIRREVERDGQVFFLHNRVQSLDDTMAFIQRLV
                     PEVMVEKAHGQMDARELEDIMHRFIHRGFHVLVSTTIIENGIDIPNVNTIIIDRADIY
                     GISQLYQLRGRVGRSDRLAYAYLFYPDGRALSELAMKRLQIISDFTDLGSGFKIAMKD
                     LEVRGAGNLLGREQSGDIYSVGFDLYLKLLDEAVARLSDEGYKPEEEPYL"
BASE COUNT          180 a          372 c          373 g          228 t
ORIGIN      
        1 caggtacggt tcctcctcgg gtttgtaccc ctcgtccgat aggcgggcga ccgcctcgtc
       61 gagcagcttg aggtacaggt cgaagccgac cgagtagatg tcgccggatt gttcgcggcc
      121 gagcaggttc ccggccccgc gcacctccag gtccttcatc gctatcttga agcccgagcc
      181 gaggtcggtg aagtccgaga tgatctgtag ccgcttcata gccagttccg acagggctcg
      241 gccgtcgggg tagaacaggt acgcgtaggc gagccggtcc gaccgaccca cgcgaccgcg
      301 cagctggtac agctggctga tgccgtagat gtcggcgcgg tcgatgatga tcgtattgac
      361 gttcggtatg tcgatgccgt tctcgatgat ggtcgtggag acgagcacgt ggaagccgcg
      421 gtggatgaag cggtgcatga tgtcctcaag ctcgcgcgcg tccatctgcc cgtgggcctt
      481 ctcgaccatc acctccggca cgagccgctg gatgaaggcc atcgtgtcgt cgaggctctg
      541 tacccggtta tggaggaaga agacctggcc gtcgcgctcg acctcccgcc gtatcgcctt
      601 ggcgatcagg tcgggctgga actcgtcgac caccgtctgg atgggatgcc tgttgttcgg
      661 cggggtcgtc agcaccgaca ggtccctgat cttgagcatc gacatgtgca gggtcctcgg
      721 tatgggcgtc gcggtcagcg tgaggcagtc gacgttggcc cgcagctcct tgagccgctc
      781 cttgtccttg acgccgaagc gctgctcctc gtcgacgatc aggagcccga ggtccttgaa
      841 cgccacgtcc ttctgcagga tgcggtgcgt cccgacgagc acgtctatcg agccctcggc
      901 gacgccggcc agcgtcttct tctgctcctt gcggtcgacg aagcgcgaca gcatgccgaa
      961 gcgcacagga aggcccctga aacggtcctg gcagttctcg tagtgctgct ccgcgagtat
     1021 cgtcgtcggc gccaggaagg cgacctgctt cccgcccatc accgccttga acgcggcgcg
     1081 catcgctatc tcggtcttgc cgtacccgac gtcgccgcag accaggcggt ccatcgggcg
     1141 ctcgctctcc atg
//
LOCUS       DAOOLN010000890         1151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_167812_length_1151_cov_2.757299, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000890 DAOOLN010000000
VERSION     DAOOLN010000890.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167812_length_1151_cov_2.757299"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PLW80_10580"
     CDS             <1..265
                     /locus_tag="PLW80_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPB67003.1"
                     /translation="LCLGIFCSAVGYLFYVASLKALGAGVSSVFINLIPVVSVAASFV
                     ILGERLSPIQLGGGAVAIGGVYLASFSGSRASARREPAQKWRQ"
     gene            complement(259..>1151)
                     /locus_tag="PLW80_10585"
     CDS             complement(259..>1151)
                     /locus_tag="PLW80_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67004.1"
                     /translation="AGLPGYYQVPAPYSDAQKKTIAELGEIMRRMLAADKELVSSAEA
                     FEEAGKDVQTIRDKAGALGEDTKANLQALSQEFDTARQMLKMCVGRQGNHFPLVTKEY
                     FHLNLREIGTRENVLDALGWLESIDCQAYCRPYKSALNRIEPFVLLLPSYGDQGICWE
                     PFDRYNRHTSRSRLAVPMYPKNLKIALATAVADMRWQAAKEKASYYWMEEGLTGQYYQ
                     WFVKQKLKGDVKEFFIQDYITWITKESDGIQKLDKEVRGIFWRLMPFSQPIKDKLRDR
                     SFVYQELYQRDINRTLSDGY"
BASE COUNT          178 a          355 c          371 g          247 t
ORIGIN      
        1 gctgtgcctg ggtatattct gctcggcggt cggctacctg ttctacgtag cgagcctcaa
       61 agcgctcggg gccggcgtgt cgagcgtgtt catcaacctg ataccggtgg tgtccgtcgc
      121 ggcgtcgttc gtcatactgg gcgagcggct gagccccatc cagctgggcg gaggcgcggt
      181 cgccattggc ggcgtctacc tggccagctt ctccggcagc cgcgcgagcg cgcggcggga
      241 gcccgcccag aagtggcgtc aataaccgtc ggataaggtg cggttgatgt cgcgctggta
      301 cagttcctgg tagacgaaac tgcggtcgcg tagcttgtcc ttgatgggct gggagaacgg
      361 catcaaccgc cagaagatgc cgcgcacctc cttgtcgagc ttctggatgc catcgctctc
      421 cttggtgatc caggtgatgt agtcctggat gaagaactcc ttgacgtcgc ccttgagctt
      481 ctgcttgacg aaccactggt agtactggcc cgtcaggccc tcctccatcc aatagtacga
      541 ggccttctcc ttggccgcct gccaacgcat gtccgcgacg gcggtggcta gggctatctt
      601 caggttcttg gggtacatcg ggacggccag cctcgagcgg ctcgtgtgcc gattgtaccg
      661 gtcgaacggc tcccagcaga taccctggtc gccgtagctc ggcagcaaca gcacgaacgg
      721 ctcgatacgg ttgagcgcgc tcttatacgg gcggcaatag gcctggcaat cgatgctctc
      781 gagccagccg agagcgtcga gcacgttctc ccgcgtgccg atctcgcgca ggttcaggtg
      841 gaagtactcc ttggtgacga gcgggaagtg gttgccctgt ctgccgacgc acatcttgag
      901 catctgcctg gcggtatcga actcctgcga cagggcctgc agattggcct tcgtgtcctc
      961 gccgagggct cctgccttgt cccggatcgt ctgtacgtcc ttaccggctt cctcgaaggc
     1021 ctcggcgctc gagacgagct ccttgtcggc ggcgagcatg cgccgcatga tctcgccgag
     1081 ctcggctatg gtcttcttct gagcgtcgga atacggcgcg ggtacctggt agtaacccgg
     1141 taggccggca t
//
LOCUS       DAOOLN010000891         1149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_168316_length_1149_cov_2.112431, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000891 DAOOLN010000000
VERSION     DAOOLN010000891.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168316_length_1149_cov_2.112431"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PLW80_10590"
     CDS             complement(<1..874)
                     /locus_tag="PLW80_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPB67005.1"
                     /translation="MKNKIVHLDGEVSSSSKSAEDVDSYLSRLSELIADQASAINQSS
                     ASIEQMSASINNIAMAAEEKLRVANELESTALEGQSEMEETEQTIKKVSDSASVIMEM
                     IQIIQDIASKTNLLAMNAAIEAAHAGDFGKGFAVVADEIRNLAESSAESAKQITQSLG
                     EVTDYIAVSTSSTEKTGRIFSDIVEQIKGVAMSMSEMKNATGELSIGAKQILEALGSL
                     VSTTEEVKGSSVDMKARVTAIVEAMRSVAAISSDTKYGMEEITIGIDEIYKAAESISR
                     AGGENSESVATLKAL"
     gene            864..>1149
                     /locus_tag="PLW80_10595"
     CDS             864..>1149
                     /locus_tag="PLW80_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67006.1"
                     /translation="MFFMPSVLMRISCRLAAVSSELGLTLLDGAEPAQAVLTLVSDKG
                     AETARLTAKVDNIAATDLAAMAPPLAFLGFVDAPISGALDARLGEDGGFAG"
BASE COUNT          152 a          379 c          370 g          248 t
ORIGIN      
        1 cgagagcctt gagcgtcgcc acgctctcgg aattctcccc tccggcccgc gagatggact
       61 cggcggcctt gtatatctcg tcgatgccga tggtgatctc ctccatgccg tacttcgtgt
      121 cgctcgagat ggccgcgacg ctgcgcatcg cctcgacgat ggccgtgacg cgggccttca
      181 tgtcgaccga cgagcccttg acctcctcgg tggtcgatac gagcgagccg agggcctcga
      241 gtatctgctt ggcgccgatg gacagctcgc cggtggcgtt cttcatctcc gacatgctca
      301 tcgccacgcc cttgatctgc tcgacgatgt cgctgaagat ccggccggtc ttctcggtgc
      361 tcgaggtgga gacggctatg tagtcggtca cctcgccgag cgactgggta atctgcttgg
      421 cgctctcggc gctcgactcc gccaggttgc gtatctcgtc ggcgacgacc gcgaagcctt
      481 tgccgaaatc gccggcgtgg gccgcctcga tcgcggcgtt catggcgagc aggttcgtct
      541 tgctcgctat gtcctggatg atctggatca tctccatgat gacggaggcg ctgtcggaga
      601 ccttcttgat cgtctgctcg gtctcctcca tctcgctctg gccttcgagc gcggtgctct
      661 cgagctcgtt ggccaccctg agcttctcct cggccgccat cgctatgtta ttgatgctcg
      721 cggacatctg ctcgatcgac gcgctcgact ggttgatggc ggaggcctgg tcggctatca
      781 gctcggacag cctggacagg tagctatcga cgtcctcggc cgacttggac gacgaggaga
      841 cctcgccgtc gaggtggacg atcttgttct tcatgccctc ggtgttgatg cgtatctcgt
      901 gcaggctggc ggcggtctcc tccgaactgg ggctgacgct gctggacggg gcagagcccg
      961 cgcaggcggt gctgacgctc gtctccgaca agggggccga gaccgcgcgg ctgaccgcca
     1021 aggtcgacaa catcgccgcg accgaccttg ccgcgatggc cccgccgctt gccttcctgg
     1081 gcttcgtcga cgccccgatc tctggcgcgc tggatgcacg gctgggcgag gacggcggct
     1141 tcgccggcc
//
LOCUS       DAOOLN010000892         1148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_168520_length_1148_cov_2.182068, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000892 DAOOLN010000000
VERSION     DAOOLN010000892.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168520_length_1148_cov_2.182068"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLW80_10600"
     CDS             complement(<1..206)
                     /locus_tag="PLW80_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HPB67007.1"
                     /translation="MDDSQNALARARAAVAGGLGSGLKTSLYLLRIMVPVSLAVALLK
                     WCGALDWAARFMAPAMGFVGLPGEA"
     gene            complement(255..>1148)
                     /locus_tag="PLW80_10605"
     CDS             complement(255..>1148)
                     /locus_tag="PLW80_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB67008.1"
                     /translation="DRGSMPVPGADTVLFGGNTACIEIRCGKRLIIIDAGSGIRSLGD
                     WLVRNELKKAPIDADVFLTHTHWDHIMGFPMFTPIYVPGTTLRIRGPVNFEDETLEQI
                     IGSQLSYRYWPVRQDELAATISYQSLKETQLDLGDGIVVRTKYLNHPVLCLGYRVEYE
                     GKAFATAYDHEPFSNLFPTDPADPDYDPVIAEEGQKAAREENERVEAFYRGVDLLVHD
                     TQYSAKEYKAGKMGWGHSSFEYAINAANRAGVGRLLLFHHDPNRTDTELQELELFYQA
                     KVAGKTRLRVDTSREGMTIQL"
BASE COUNT          184 a          358 c          386 g          220 t
ORIGIN      
        1 gcctcgcccg gaaggccgac gaagcccatc gccggcgcca tgaagcgggc ggcccagtcg
       61 agcgcgccgc accacttgag cagcgcgacg gccaggctga ccggtaccat gatccggagc
      121 aggtagagcg acgtcttgag ccccgagccc agtccgcccg cgacggcggc gcgcgcccgg
      181 gcgagcgcgt tctgagagtc atccatcggg ccggattctc ctcgcatagg cccctcctgt
      241 caaggtcggg cgggctatag ctgtatcgtc atgccctctc ggctcgtatc cacccgtagc
      301 cgcgtcttgc ctgcgacctt ggcctggtag aacagctcaa gctcctgcag ctcggtgtca
      361 gtgcggttcg ggtcgtggtg gaagagcagg agccgcccga cgccggcgcg gttggcggcg
      421 ttgatggcgt actcgaacga cgaatggccc caacccatct tgccggcctt gtactccttg
      481 gcgctgtact gagtgtcgtg cacgagcaga tcgacgccgc ggtagaaggc ctcgacgcgc
      541 tcgttctcct cacgtgcggc cttttggcct tcctccgcta taaccgggtc gtagtcggga
      601 tcggccggat cggtggggaa caggttcgag aatggctcgt ggtcgtaggc cgtggcgaag
      661 gccttgccct cgtattcgac gcggtagccg aggcagagca ctggatggtt caggtatttt
      721 gtcctgacga cgatgccgtc gccgaggtcg agctgcgtct ctttgagact ctggtagctg
      781 atcgtggctg cgagctcgtc ctgcctgacc ggccagtatc ggtacgacag ttgggagccg
      841 atgatctgct cgagtgtctc gtcctcgaaa ttgaccggac cgcggatgcg caacgtggtg
      901 ccgggcacgt agatcggcgt gaacatcggg aaacccatga tatggtccca gtgggtatgc
      961 gtcaggaaga cgtcggcgtc gataggggcc tttttgagct cgttgcggac cagccagtcg
     1021 ccgaggctcc tgatgcccga gccggcgtcg atgatgatga gccgcttccc gcaccgtatc
     1081 tcgatacagg cggtattgcc gccgaacagc accgtatcgg cgccaggtac cggcatcgag
     1141 ccgcggtc
//
LOCUS       DAOOLN010000893         1146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_169050_length_1146_cov_1.980752, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000893 DAOOLN010000000
VERSION     DAOOLN010000893.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169050_length_1146_cov_1.980752"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PLW80_10610"
     CDS             <1..546
                     /locus_tag="PLW80_10610"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPB67009.1"
                     /translation="ARVEEAYSMMKELAERDELTGLRNRRSGANRLALENARSSRSSA
                     PFCVAIGDIDHFKAFNDLHGHETGDLVLKHVAGAVERSVRMEDMAARWGGEEFLILLS
                     GDSLPAALEASDRIRKAVSAEAVTVGTERLHISMTIGVAEFAKGEPIDELIARADAAM
                     YKGKAAGRDIVVAAPPPRARS"
     gene            complement(541..816)
                     /locus_tag="PLW80_10615"
     CDS             complement(541..816)
                     /locus_tag="PLW80_10615"
                     /inference="COORDINATES: protein motif:HMM:NF040785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3324 family protein"
                     /protein_id="HPB67010.1"
                     /translation="MPYLNADGVLPPELLSEIQKYVQGALVYVPRQGPERLGWGQKNG
                     ARDELDRRNAAIREAKACGRTIDELADEYCLSADGIRKILYCNRRAS"
BASE COUNT          227 a          348 c          352 g          219 t
ORIGIN      
        1 gccagggtcg aggaagccta ttcgatgatg aaggagctgg ctgagcgcga cgaactgacc
       61 ggactacgca accgccgatc gggggccaac cggcttgcgc tcgagaacgc gcgttcgagc
      121 cgttcctccg cgccgttctg cgtggccatc ggggacatcg accacttcaa ggcgttcaac
      181 gacctgcacg gccacgagac cggggacctc gtgctcaagc atgtcgcggg cgccgtcgag
      241 cgcagcgtca gaatggaaga catggctgcg cgctggggcg gcgaggaatt cctgatcctg
      301 ctctcgggtg actcgctgcc cgccgcgctc gaggccagcg accgaatacg caaggcagtg
      361 tcggccgaag ccgttacggt ggggacggag aggctccata tatcgatgac gataggcgta
      421 gcggagttcg ccaagggcga gcctatcgac gagctgatag ccagggccga cgctgccatg
      481 tataaaggaa aagcggcggg acgcgacatc gtcgtcgcag ccccgccgcc ccgagcgcgc
      541 tcatgaagcg cgccggttac agtataggat cttcctaatg ccgtcggccg ataggcagta
      601 ctcgtcggcc agctcatcga tggtacgtcc gcaggccttc gcctctcgaa tagccgcgtt
      661 gcgcctgtcc agctcgtccc tggcgccgtt cttctggccc catccgagac gctccggccc
      721 ctgcctgggt acatagacca aggcgccctg cacgtacttc tgtatttcgc tcagtaattc
      781 gggaggcaat acgccgtccg cattcaaata tggcatgatc gttcctcgaa ggatgtacgt
      841 tacatccgcc aatgaccgcg aggaggaacg agctatggtg ggctttcctc ttcgctacgc
      901 ctcggcggcg ccatgggtca gggggctatc gactgctcgg tttcggatca ttatcatagt
      961 gaacctccat gtatcgcgaa gatgcggagc atcctatcac aagcccacat acgtgtaaac
     1021 ggatcgaatt gatataatcg atcattccac tcgcctgggg aggaaagatc cgatagacgc
     1081 gtactgtcgc ttttccgggc ggcggcgccg tggcatagtg atcatgccgg cattaccaac
     1141 atcttg
//
LOCUS       DAOOLN010000894         1145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_169154_length_1145_cov_3.481651, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000894 DAOOLN010000000
VERSION     DAOOLN010000894.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169154_length_1145_cov_3.481651"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="PLW80_10620"
     CDS             complement(<1..569)
                     /locus_tag="PLW80_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67011.1"
                     /translation="MDRRAYDRFSELRDEFKARVAAWSAALPGLADAQRRLAVETGDT
                     GYAVETPIVYNRALDEVGPGDDIAWLVVADNPGKREQEAAMNRYLVGRSGQAAERFFA
                     RELGIDFRRQVVVVNKTPVHTPKTAQLRKLARIIPEAGPVLEESQRHMAALVPELQAC
                     LGARVWVMGLSELRPKGLFKAWRAALNEA"
     gene            717..>1145
                     /locus_tag="PLW80_10625"
     CDS             717..>1145
                     /locus_tag="PLW80_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB67012.1"
                     /translation="MSRIIVFVNQKGGVGKTTSVINLGAYLARAGKRVLLVDFDPQAN
                     LTSGVGGDKTALGAYDVISGNAVMRDVVKPTKVDGLFLAPSSIALSGATVELVDKEGR
                     DDYLKNALAPVRGDYDFILIDCPPSLGVLTLNGLSAANEVL"
BASE COUNT          184 a          405 c          358 g          198 t
ORIGIN      
        1 taggcctcgt tcagcgcggc ccgccaggcc ttgaagaggc ccttgggtcg cagctccgat
       61 aggcccatca cccagactct cgccccgagg caggcctgca gctcgggcac gagggccgcc
      121 atatgtcgct ggctctcttc gagcaccggc ccggcctcgg gtatgatcct ggcaagcttg
      181 cgtagctggg ccgtcttcgg ggtatggacc ggcgtcttgt tgacgacgac aacctggcgc
      241 ctgaagtcga tgccgagctc ccgcgcgaag aagcgctcgg cggcctgacc ggatcggccg
      301 accaggtagc ggttcatggc agcttcctgt tcgcgcttgc ccgggttgtc ggccaccacc
      361 agccacgcga tgtcgtctcc cggcccgacc tcgtcgagcg cgcggttgta gactatcgga
      421 gtctcgacgg cgtagccggt atcgccggtc tccacggcca ggcgcctctg ggcgtcggcc
      481 aagccgggca gggcggcgga ccacgcggcg acccgcgcct tgaactcgtc cctgagttcg
      541 ctgaacctgt cgtacgcccg tctgtccacc gtgcccgcct tcgtacctaa gcttcgcaac
      601 actaacccgg gctggccccg atccgcaagc gcgggcgata gtcgatcgta tgccgtagcc
      661 cttgccgcgc tttccatgct atcctgatct atacctgaga accccaagga cgaacgatgt
      721 ccaggataat tgtattcgtt aaccagaagg gcggcgtcgg caagacgacg tccgtgatca
      781 acctcggagc ctacctcgcc cgcgcgggca agcgcgtcct gctcgtcgac ttcgacccgc
      841 aggccaacct gaccagcggc gtcggcggcg acaagacggc cctgggcgcc tacgacgtca
      901 tctccggtaa cgcggtcatg cgcgacgtgg tcaagccgac caaggtggac ggcctgttcc
      961 tggccccgtc atcgatcgcg ctgtccggcg ccaccgtcga gctcgtcgac aaggaaggcc
     1021 gcgacgacta cctcaagaac gcgctggcgc cggtccgggg cgactacgac ttcatcctga
     1081 tagactgccc gccgtcgctc ggcgtactga cgctcaacgg cctatcggct gcgaacgagg
     1141 tgctc
//
LOCUS       DAOOLN010000895         1145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_169173_length_1145_cov_3.046789, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000895 DAOOLN010000000
VERSION     DAOOLN010000895.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169173_length_1145_cov_3.046789"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PLW80_10630"
     CDS             <1..359
                     /locus_tag="PLW80_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HPB67013.1"
                     /translation="ELRHLDSPLLDDSLDPFLRDLHDNAVQVIEAIESYRETASGIQE
                     VFLSSVSNRMNEVMKVLTIISTIFIPLTFIAGVYGMNFRHMPELDQPWAYPAAWGLMV
                     AIAMGMITFFKKKKWF"
     gene            608..>1145
                     /locus_tag="PLW80_10635"
     CDS             608..>1145
                     /locus_tag="PLW80_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HPB67014.1"
                     /translation="MVTKTFKGGTHPPERKERSASTPIQTLRNVSQVVIPVNQHFGAP
                     IQPLVKVGDQVKRGQKIADADGRMTVPLHASVYGTVKKIEPRMQANNLEGLAIVIEPL
                     PGDEAVFMPTLDPFSCTKEEALARVREAGIIGVGGAGFPAHVKFAPPPNKPIEYVIAN
                     AAECEPYLTIDERVMEEHP"
BASE COUNT          246 a          349 c          339 g          211 t
ORIGIN      
        1 tcgagctgcg gcacctggat tcgccgctcc tggacgatag cctggacccg ttcctccgcg
       61 acctgcacga caacgcggtc caggtgatcg aggcgatcga gagctatcgg gagacggcgt
      121 cgggcatcca ggaggtcttc ctctcgtcgg tatcgaaccg catgaacgag gtcatgaagg
      181 tgttgacgat catttcgacg atcttcattc ccttgacctt catcgccggt gtgtacggca
      241 tgaatttccg gcatatgccc gaactcgatc agccgtgggc gtatcccgcg gcgtggggcc
      301 tcatggtggc gatagctatg ggaatgatca cattctttaa aaaaaagaag tggttctaat
      361 ctcttaaaga ttatcgttag ttttctattt atatactgta aaaagcgatg cttgacggta
      421 tacgccgctc gcgctacgat agggcccgtc ctgctcgtac ccgcggctga accgtctcag
      481 cagagctagc cgaaggctcg cccgcggcgt atgcgagcgc catcaccgga cccgttcata
      541 gccccaaagt cagcgtcgcg gagcctaggc cccgcggtag caacggaaga aggagcccct
      601 cctcgccatg gtgacgaaaa cattcaaagg cggaacgcat ccgccagagc gcaaggaacg
      661 aagcgcatct acgccgatac agaccttgcg caacgtatcg caggtggtca tcccggtcaa
      721 tcagcacttc ggcgcgccta tccagcccct cgtcaaggtc ggggaccaag taaagcgcgg
      781 tcagaagatc gcggacgccg acgggcgcat gaccgtcccg ctccacgcgt cggtctacgg
      841 caccgtcaag aagatcgagc cgaggatgca ggccaacaat ctcgagggcc tggccatcgt
      901 catcgagccc ctgcccggcg acgaagcggt gttcatgccg acgctcgatc ccttcagctg
      961 cacgaaggaa gaagcgctcg ctcgcgtacg cgaagcgggt atcatcggcg tcggcggcgc
     1021 tgggtttccg gctcatgtca aattcgctcc gccgccgaac aagccgatcg agtacgtgat
     1081 agcgaacgcg gccgagtgcg agccctacct gacgatagac gagcgcgtga tggaggagca
     1141 tcccg
//
LOCUS       DAOOLN010000896         1144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_169441_length_1144_cov_3.105601, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000896 DAOOLN010000000
VERSION     DAOOLN010000896.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169441_length_1144_cov_3.105601"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PLW80_10640"
     CDS             complement(<1..237)
                     /locus_tag="PLW80_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67015.1"
                     /translation="MMRSAVDSIIGLVDRIDSIERETEDIYLRLGTVFHAVKKAVDAS
                     AASAEQTIAAVLSQHRGGQSADVAKRRSEDFIDDA"
     gene            complement(324..>1144)
                     /locus_tag="PLW80_10645"
     CDS             complement(324..>1144)
                     /locus_tag="PLW80_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB67016.1"
                     /translation="LKLRKVPREEIARRVEEAAEMLGIQQLLKRRPKELSGGQRQRVA
                     VGRAIVRNPKVFLFDEPLSNLDAKLRVQMRAEIIDLHQRLQATMIYVTHDQVEAMTMG
                     DKIVVMKDGLVQQIGSPLYLYNHPINKFVAGFIGSPPMNFVTVKVLEEGGRLVLDEGS
                     SRMTPSAEHAPSLKAYVGKEVVLGLRPEDLPYDETGTGFKTTISVVEPLGAEIHLWAK
                     TSSSQIVSKVPPHHTFKIGETVCFAPVMEKARYFDPVSERSILPVAEVEGPSPN"
BASE COUNT          190 a          368 c          373 g          213 t
ORIGIN      
        1 agcgtcgtcg atgaagtcct cggagcggcg tttggccacg tcggcgctct ggccgccgcg
       61 atgctggctg agtacggccg cgatagtctg ctcggcggag gcggcgctcg cgtccaccgc
      121 cttcttgacg gcatggaaca cagtacccag gcggaggtag atatcctccg tctcgcgctc
      181 tatcgagtct atcctgtcta ccaggccgat tatcgaatca accgcggatc gcatcatgca
      241 cctactatag agaatccggt cgccagttac aagaacgggc cgccccggag ggcggcccgt
      301 cgatagcggc tccgagacgc ggttcagttc ggcgacgggc cttcgacctc ggccaccggc
      361 aggatggacc gttcgctgac aggatcgaag taacgggcct tctccatgac cggagcgaaa
      421 cagacggtct ccccgatctt gaaggtgtgg tgcggcggca ccttggacac gatctggctg
      481 gagctggtct tggcccacag gtggatctcg gcgccgagcg gctcgacgac gctgatcgtc
      541 gtcttgaagc ccgtaccggt ctcgtcgtag ggcaagtcct cggggcggag gccgagcacg
      601 acctccttgc cgacgtaggc cttgagcgac ggggcatgct cggccgacgg cgtcatccgg
      661 ctcgaaccct cgtcgagcac gagcctgccg ccctcctcga gcaccttgac ggtgacgaag
      721 ttcatcggcg gcgagcctat gaagccggcg acgaatttgt tgatcgggtg gttgtacagg
      781 tacaggggcg aaccgatctg ctgcacgagg ccgtccttca tgacgacgat cttatcgccc
      841 atcgtcatgg cctcgacctg gtcgtgggtc acgtagatca tcgtcgcctg caagcgctga
      901 tgcagatcga tgatctcggc gcgcatctgc acgcgcagct tggcgtccag gttggacaaa
      961 ggctcgtcga acaggaagac cttcgggttg cgcacgatgg cgcggccgac cgcgacgcgc
     1021 tggcgctgtc cgccggacag ctccttgggc cggcgcttca gaagctgttg gatgcccagc
     1081 atctcggcgg cttcctcgac gcgccgcgcg atctcctccc gcggcacctt gcgcagcttg
     1141 agcc
//
LOCUS       DAOOLN010000897         1144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_169458_length_1144_cov_2.844812, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000897 DAOOLN010000000
VERSION     DAOOLN010000897.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169458_length_1144_cov_2.844812"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..999)
                     /locus_tag="PLW80_10650"
     CDS             complement(214..999)
                     /locus_tag="PLW80_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67017.1"
                     /translation="MNDEKHLYLIMHPNHALIASQLDPERFAKHYTQGSTRYFEGRLI
                     FVEIDPSFRHPYFNIDAAFAELKPHEDGRPKATKFISSYRTFEHMDFNAFGKLYYCNS
                     LGDYVELESAAYDPKMRGDEMRIVLEINPIKMMVLTKYNFLEYAKFITDPDMPKGAPV
                     MFYTQLEFTVDEFLKEFQENPFIRCFVPGIHPARLREAIFEVRAKPGKNTKGLSLDCP
                     VDRISYKFLRHGFMFASATQTKFYPLMSLEDAERKYYKFWKNM"
     gene            complement(1004..>1144)
                     /locus_tag="PLW80_10655"
     CDS             complement(1004..>1144)
                     /locus_tag="PLW80_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HPB67018.1"
                     /translation="AHKAAVVGDTVGDPFKDTAGPALNILIKLMAIISLVIAPMLKSY
                     WG"
BASE COUNT          209 a          320 c          378 g          237 t
ORIGIN      
        1 agagcaagaa gaagaacgtg ctcgtggagg gcgtgacgtc gctggaccag tacaagctgc
       61 tccgcgggct cggggccgcg tacctgcagg gctactactt cagcgagcca cggtcgggcg
      121 acgacttcct cgccctgctc aaggccggga ggccgctacc cgtgtcggcg tgacgatggg
      181 cgcgggaccc tgacgagccc gcgccgcatc ttcctacatg ttcttccaga acttgtagta
      241 cttgcgctcg gcgtcctcga gggacatgag cgggtaaaac ttggtctggg tagcggacgc
      301 gaacatgaag ccgtggcgca ggaatttgta cgagatgcgg tcgaccgggc agtcgaggga
      361 cagtcccttg gtattcttac ccggcttggc ccgcacttcg aagatcgcct ccctgagcct
      421 ggcggggtgg atgcccggca cgaagcagcg gataaagggg ttttcctgga attccttgag
      481 gaactcgtcg acggtgaact cgagctgggt atagaacatc accggcgcgc ccttgggcat
      541 gtccgggtcg gtgatgaatt tggcgtactc caggaagttg tacttggtca gtaccatcat
      601 cttgatcgga ttgatctcga ggacgatgcg catctcgtcg cctcgcatct tggggtcgta
      661 ggcggccgac tctagctcga cgtagtcgcc gagcgagttg cagtagtaca gctttccgaa
      721 ggcgttgaag tccatgtgct cgaaggtgcg gtagctcgag atgaacttgg tcgccttcgg
      781 gcggccgtcc tcgtggggct tgagctccgc gaaagccgcg tcgatattga agtacggatg
      841 cctgaaggac gggtcgatct cgacgaagat caatcgcccc tcgaagtacc gggtcgagcc
      901 ctgcgtatag tgcttggcga aacgctccgg atccagctgg ctggcaatca gcgcgtggtt
      961 cgggtgcatg atcagataca ggtgcttctc gtcgttcatc tcgctacccc caatatgact
     1021 tgagcatcgg cgcgatcacc agggagatga tcgccatcag cttgatcagg atgttgagcg
     1081 cgggcccggc cgtgtccttg aacgggtcgc cgacggtgtc gccgacgacc gccgccttgt
     1141 gggc
//
LOCUS       DAOOLN010000898         1143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_169678_length_1143_cov_3.282169, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000898 DAOOLN010000000
VERSION     DAOOLN010000898.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169678_length_1143_cov_3.282169"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..>1143
                     /locus_tag="PLW80_10660"
     CDS             130..>1143
                     /locus_tag="PLW80_10660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPB67019.1"
                     /translation="MVKNIQRKEEDAKRMAEMMVSNMHEINAENIRGLERTRSEYATR
                     DDSGENWRMMLGDCVERIKELPDDSVHFSVFSPPFASLYTYSNSDRDMGNSKDEAEFA
                     RHFQFLVKELYRVIKPGRLVSFHCMNLPTTKTHHGYIGLVDFRGDLIRAFEAEGFIYH
                     SEAVIWKDPVTAMQRTKAIGLLHKQLVKDSAMSRQGVPDYLVTMKKPGENTEPIEGEL
                     DHYSGDGNFISTGRLSIDLWQLYASPVWMDINPSDTLQYRSARDEKDERHICPLQLQV
                     IHRALQLWSNPGDVVLSPFAGIASEGYESIKLGRKFIGIELKPSYYAQAVKNLAAIEN
                     HVEN"
BASE COUNT          310 a          274 c          303 g          256 t
ORIGIN      
        1 cataatgttt tttttgtagg actttctgat tcctatgagc ttttctatca agccattcgc
       61 cgatgctggc gcttcggcca gaatagcacg gtaaagtgct acgtcatcac tagcgacatc
      121 gagggcgccg tggtaaagaa cattcagaga aaagaggagg acgcaaaacg aatggctgaa
      181 atgatggtgt caaatatgca cgaaataaac gccgaaaaca tacgcggcct tgagcgcacg
      241 aggagcgagt acgccacgag agacgacagt ggcgaaaact ggcgcatgat gcttggagat
      301 tgcgtcgagc gaataaagga actgcccgat gattctgtcc acttctcggt attctcgccg
      361 ccgttcgcgt cactctatac ctactccaat tccgataggg atatggggaa ctcgaaagac
      421 gaagcggaat tcgcgcgtca ttttcagttt cttgtcaagg aactataccg cgtcataaag
      481 ccaggtcggt tggtatcatt ccattgcatg aacctcccaa cgacaaagac gcaccatgga
      541 tatatcggcc ttgtcgattt taggggcgac ctaatacgcg catttgaggc cgagggtttt
      601 atttaccata gcgaggccgt gatatggaaa gatcctgtta ccgctatgca gaggacgaag
      661 gccatagggc tgctccataa acagttggtt aaggattctg ctatgtcgcg ccaaggcgta
      721 ccagactacc tggtaacgat gaagaagccc ggagaaaaca cggagcctat agagggcgag
      781 cttgaccatt attcaggcga cggaaatttt atatcgaccg gccgactgtc catagacctt
      841 tggcagcttt acgcgagtcc ggtatggatg gatataaacc ccagcgacac gctacagtat
      901 cgaagcgcca gagacgagaa agacgagcgt catatatgcc cgctccagct ccaggttatt
      961 catcgcgcct tgcagttgtg gtctaaccct ggcgacgttg tactgtctcc gtttgccggt
     1021 atcgcatctg agggatacga gtctatcaag ctgggccgga agtttatcgg catagagtta
     1081 aaaccaagtt actacgcgca ggcggtaaaa aacctggccg cgatagaaaa ccacgtggaa
     1141 aac
//
LOCUS       DAOOLN010000899         1143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_169709_length_1143_cov_2.758272, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000899 DAOOLN010000000
VERSION     DAOOLN010000899.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169709_length_1143_cov_2.758272"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1143)
                     /locus_tag="PLW80_10665"
     CDS             complement(<1..>1143)
                     /locus_tag="PLW80_10665"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 family protein"
                     /protein_id="HPB67020.1"
                     /translation="VFRFLYRWPLKPFIYALIPLLVAVTFFFDADPNLRAVAGLAWDT
                     GGVTTGPVTVPLVIALGIGVSRIVGSRSDASGGLGVVTLASALPVAAVFALAAALVPS
                     VPAPGSPAAFFSKAERERAVFVAGSEEALAELASGAVASGSLAASDFVALFPDYYQLA
                     GTSAGDASDGGTASGGLRARLGDSALSALKAVLPLAFVLIVTLVFLLKDRLVGIDELV
                     LGLVFAVLGMFLFNFGMEKGLTALGTQAGSSLPRAYERTERPDRALLLSGVTEADVVK
                     AVGPDGPAEYIWMDGTNGPVAVPFDRALWDEASGTYRYVPVERAVFDRWGENAGFAAA
                     LLFVFILGFGATLAEPSLSALGTTVEDLTTGTYKKSTLVGMVAVGVG"
BASE COUNT          217 a          403 c          389 g          134 t
ORIGIN      
        1 gccgacgccg accgcgacca tgcctacgag cgtcgacttc ttataggtgc cggtcgtcag
       61 gtcctcgacc gtcgtaccga gggccgacag cgacggctcg gcgagcgtcg cgccgaagcc
      121 gaggatgaac acgaacagga gggccgccgc gaagccggcg ttctcgcccc atctatcgaa
      181 gacggcacgc tcgaccggca cgtagcggta cgtgccgctc gcctcgtccc acagggcccg
      241 gtcgaagggc acggcgaccg gcccgttcgt tccgtccatc cagatgtatt cagcgggacc
      301 gtcaggccct acagccttga cgacatccgc ctcggtaacg ccgctcagga gcagcgcccg
      361 gtcgggccgc tcggtccgct cgtaggcgcg cggcaggctg gacccggcct gggtaccgag
      421 cgcggtcagg cccttctcca tgccgaagtt gaacaggaac atgccaagca ccgcgaaaac
      481 caggccgagc acgagctcgt cgataccgac caaccggtcc ttgagcagga acacgagcgt
      541 gactatcagc acgaacgcca gtggcagcac ggccttgagg gccgacaggg cgctatcgcc
      601 gagacgggcg cggaggcccc cgctcgctgt ccctccgtca gaggcgtcgc cggcggaggt
      661 cccggcgagc tggtagtagt cggggaacag cgccacgaaa tcggaggcgg ccaggctacc
      721 cgacgccacg gccccggagg ccagttccgc gagcgcctcc tcggagccgg cgacgaacac
      781 ggcccgctct cgctcagcct tcgaaaagaa cgcggccggg cttccgggag cgggcacgct
      841 cggcaccagg gcagccgcca gggcgaatac cgccgccacc ggtaacgccg aggcgagcgt
      901 caccacgccc aggccgccag aagcatcgct acgcgaaccg acgatgcgcg agacgccgat
      961 gccgagggca atgaccagcg gcacggtcac cggccccgtg gtcacgccgc ccgtgtccca
     1021 ggccagaccg gccacggccc tcaggttcgg gtcggcgtcg aagaagaagg tcaccgcgac
     1081 gagtagcggt atcagtgcgt agatgaatgg cttgaggggc cagcgataca ggaagcggaa
     1141 gac
//
LOCUS       DAOOLN010000900         1141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_170154_length_1141_cov_3.350829, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000900 DAOOLN010000000
VERSION     DAOOLN010000900.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170154_length_1141_cov_3.350829"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="PLW80_10670"
     CDS             <1..727
                     /locus_tag="PLW80_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB67021.1"
                     /translation="GMLFSLLHAFTSIHLKADQIISGTALNILASGLTIYLCQILFNQ
                     QRTRAFSYGLRKVTVPILSKIPIIGPMLFYEMYPTFYLAITLVVVTWFVVFKTPFGLR
                     LRSCGEHPQAAASMGINVYAMRYAGVIASGAFAGLAGATMVLTQDIQYTVTSIHGTGF
                     IALASLVFGKWNPWGVLGAGMFFGFSQTLSYYAKDIKILASLPLEVFYALPYVLTIVA
                     LVVFAGKSVGPKAAGEIYDQGKR"
BASE COUNT          172 a          387 c          366 g          216 t
ORIGIN      
        1 cggcatgctg ttctcgctcc tgcacgcgtt cacgagcatc catctcaagg ccgaccagat
       61 catctccggc acggcgctga acatactggc gagcggtctg accatttacc tctgccagat
      121 cctgttcaac cagcagcgca ctcgcgcgtt cagctacggt ctgcgcaagg tgacggttcc
      181 gatcctatcc aagataccga tcatcgggcc gatgctattc tacgagatgt acccgacgtt
      241 ctacctcgcg atcacgctcg tcgtcgtcac ctggttcgtc gtgttcaaga cgccgttcgg
      301 gctccgcctg cgctcttgcg gcgagcatcc ccaagcggcc gcgtcgatgg gcatcaacgt
      361 ctacgcgatg cgctacgcag gcgtcatcgc ctccggcgcc ttcgccggat tggccggggc
      421 gacgatggta ctgacccagg atatccagta cacggtcacc tccatccacg gcaccggctt
      481 catcgctcta gcgtcgctcg tattcggcaa gtggaacccc tggggcgtcc tcggcgcagg
      541 catgttcttc ggcttctccc agacgctgtc gtactacgcc aaggacatca agatattggc
      601 gtccttgccg ctcgaggtgt tctacgcgct accctacgtg ctgaccatcg tggcgctcgt
      661 cgtattcgcc ggcaagtccg tggggccgaa ggccgcgggc gagatttacg atcagggaaa
      721 gcgttaggcg atcgccgacg cttcgcgcgg cgatcgctcc cgcgggttca accggactcg
      781 ctggcgctcg ccggttaact ccgcgggccg gtacgacggg tgaccgagct cgctgcgctc
      841 gccggtcatg gggcggtagc ggcgggcaac cggactcgct gcgctcgccg gttaactccg
      901 cgggccggta cagcgggtga ccgagctcgc tggcgctcgc cggtcatggg gcagtagcgg
      961 cgggcaaccg gactcgctac gctcgccggt taactccgag ggccggtacg gcgggtgacc
     1021 gagctcgctg gcgctcgccg gtcatggggc ggtagcggcg ggcaaccgga ctcgctgcac
     1081 tcgccggtta actccgcggg ccggtacggc gggtgaccga gctcgctgcg ctcgccggtc
     1141 a
//
LOCUS       DAOOLN010000901         1141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_170230_length_1141_cov_2.480663, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000901 DAOOLN010000000
VERSION     DAOOLN010000901.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170230_length_1141_cov_2.480663"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PLW80_10675"
     CDS             complement(<1..604)
                     /locus_tag="PLW80_10675"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPB67022.1"
                     /translation="MKATRGHAAILVNPQKDDAERLSREIDAALCAESWSTELVSFGR
                     GSVDGSSIADANILVCLGGDGTVLYAGRRAAPFGVPILPVNLGTLGFIAWVKRDEWKE
                     RFDEAVSGGLAVSERFMLDVSVERDGNAVAGFSALNDGVISGSGPAKIVELCVSVNGA
                     RLGKYRSDGLIVSTPTGSTAYSLASGGPVLDPDMEAMVFTP"
     gene            complement(617..1012)
                     /locus_tag="PLW80_10680"
     CDS             complement(617..1012)
                     /locus_tag="PLW80_10680"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPB67023.1"
                     /translation="MNDMNAALAASQLKDLERFIEKRRELADLYAQSLARAHRKPLAP
                     TIECEPSNFGCVVVLDSGVKDVRAYARKKDVDTAMAFDDTCVAAGFVPDGACPIAASL
                     ANRSVAFPMNQRVGKSAAQKIAKVLATLP"
BASE COUNT          206 a          370 c          357 g          208 t
ORIGIN      
        1 tgggagtaaa gaccatcgct tccatgtccg ggtcgaggac cggcccgccg gacgccagcg
       61 aataggcggt cgaacccgtc ggagtcgaga ctatgaggcc gtcggagcgg tatttgccta
      121 gcctagcccc atttaccgaa acgcatagct cgacgatttt ggcagggccg gaaccggata
      181 tgacaccatc gttgagggcg ctgaagcccg ctacggcgtt gccgtcgcgc tcgaccgaca
      241 cgtcgagcat gaagcgctcc gataccgcga ggcccccgga taccgcctca tcgaagcgtt
      301 ctttccattc atcgcgcttc acccaggcta tgaagccgag cgtgcctaga ttgaccggca
      361 gtatcggaac gccgaacggg gcggccctgc gcccagcata gagtaccgta ccatcaccgc
      421 ctaggcagac gagaatgttc gcgtccgcta tggacgatcc atcgacggaa cccctgccga
      481 acgatacgag ctccgtcgac caggactcgg cgcatagggc cgcgtcgatc tcccgcgaca
      541 gacgctcggc gtcgtccttc tgcgggttta cgagtatcgc cgcgtgtcct cgcgtcgctt
      601 tcatgcgtcc tctcaatcag ggcagggtag cgagcacctt ggcgatcttc tgcgcggcgc
      661 tcttgccgac gcgctggttc atcgggaagg cgaccgatcg gttcgccagc gacgcggcga
      721 tggggcaggc tccatcgggg acgaagccag ccgcgacgca ggtatcgtcg aaggccatcg
      781 cggtatcgac gtctttcttc ctggcgtagg cgcgcacgtc cttgacccct gagtcgagta
      841 ccacgacgca gccaaaattc gacggctcgc attcgatagt cggtgcgagc ggcttacgat
      901 gagcgcgtgc cagtgactgg gcgtatagat cggccagctc ccgccgcttc tcgatgaacc
      961 gttccaggtc cttgagctgc gaggccgcca acgccgcgtt catatcgttc atgagctcct
     1021 cgggcggcaa gccctcggac gcgttccgga gtgcctgggc ctcgcgcctg ccgggtgagt
     1081 acaacaacgc gcctcctccg gcagtgatcg acgaggcagg ttcgaggctg atgatgccgt
     1141 a
//
LOCUS       DAOOLN010000902         1140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_170406_length_1140_cov_3.011060, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000902 DAOOLN010000000
VERSION     DAOOLN010000902.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170406_length_1140_cov_3.011060"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PLW80_10685"
     CDS             <1..472
                     /locus_tag="PLW80_10685"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HPB67024.1"
                     /translation="GGAGPVRSIVMTVADCMPIFVYDRDSGAFGLLHSGWKGTGILAE
                     AVRLMGRLYGSRPGSLSVAFGPCIGSCCYVVDEARARAFESEFGSTAVDRPDGRPRLN
                     LVEANRALADALGLVDPSIAGDCTCCDGRFGSFRRQGPSSFTRMAVAIGHTESV"
     gene            469..>1140
                     /locus_tag="PLW80_10690"
     CDS             469..>1140
                     /locus_tag="PLW80_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67025.1"
                     /translation="MTATRGLRCAIRVHRCVSRGLACALALSAACVAQAQAGLTPFNP
                     PEGRVIVTERSDFSRYEDGRYVGHAYREARLDLSLTRTAEGLARYSGEALVLEETMRD
                     ERAVARRLDEAVPVEFTAGPDGTLRFSVDRGYPILRGVPTSPPEDVAIGGRWTGYGSV
                     VVRPRPGHSATRVRVLVEYEYRGPTNYAGRAAEAIRARYAVRYDGRDPSGDPDMSGAT
                     GGRTAD"
BASE COUNT          159 a          392 c          405 g          184 t
ORIGIN      
        1 cggcggcgcc ggcccggtcc gctccatcgt catgacggtc gccgactgca tgccgatctt
       61 cgtttacgac cgggactcgg gcgccttcgg gctcctccat tctggctgga aggggacggg
      121 catcctggcc gaggcggtcc ggctcatggg gcggctctac ggttccaggc ccgggagcct
      181 gagcgtcgcc ttcggaccat gcatcggttc gtgttgctac gtggtcgacg aggcccgggc
      241 tcgcgccttc gagtcggaat tcggctctac agcggtcgat aggccggacg gaaggccccg
      301 gctgaacctc gtcgaggcga accgggcgct ggccgacgcg ctcgggctgg tcgacccatc
      361 gatcgccggc gactgtacct gttgcgacgg ccgcttcggc tccttcaggc ggcagggacc
      421 gtcgtcgttc accaggatgg ccgtggccat cgggcatacg gagtcggtat gaccgcaacc
      481 cgcggcctca gatgcgcgat acgcgttcat agatgcgtgt cgcgcggtct cgcgtgcgct
      541 ctggcgctat cggccgcctg tgtcgcgcag gctcaagctg gcctcacgcc cttcaacccg
      601 cccgagggtc gagtcatcgt caccgagcga tcggacttct cgcgctacga ggacggccgc
      661 tacgtcggac atgcctaccg ggaggcgagg ctcgacctgt cgctgaccag gaccgccgag
      721 ggcctggccc gctactcggg cgaggccctc gtactcgagg agacgatgcg cgatgagagg
      781 gcggttgcca ggcgcctcga cgaggccgtg ccggttgagt tcaccgccgg gccggacggt
      841 acgctacgct tctcggtcga ccgaggctat ccgatactcc gcggagtgcc gacctcgcca
      901 cccgaggacg tggccatagg cggccgctgg actggctatg gctcggtcgt cgtcaggcca
      961 cggcccggcc atagcgcgac gagggtgcgg gtgctcgtcg agtacgagta tcgaggcccc
     1021 acgaactacg ccggtcgcgc cgccgaggcg atcagggcgc gctacgccgt ccgatacgac
     1081 gggcgcgatc cctcgggcga cccggacatg agcggcgcca ccggcggccg taccgccgat
//
LOCUS       DAOOLN010000903         1138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_170902_length_1138_cov_2.580794, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000903 DAOOLN010000000
VERSION     DAOOLN010000903.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170902_length_1138_cov_2.580794"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..375)
                     /locus_tag="PLW80_10695"
     CDS             complement(82..375)
                     /locus_tag="PLW80_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HPB67026.1"
                     /translation="MLTSKQRARLSSLANELNPIVFLGKAGPVDGVHEALDRALTDHE
                     LVKLRFVDFKGERKELARELAEKAGAELVRVIGHVAIIYRPSPDPQKRAIELD"
     gene            complement(388..969)
                     /locus_tag="PLW80_10700"
     CDS             complement(388..969)
                     /locus_tag="PLW80_10700"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HPB67027.1"
                     /translation="MLRTVLGLLSAATSIYMLLCVVRVFMSWLPSARLGKPAELVERA
                     TEPYLSWWRRFPILRSGGVDFSPIAALAALSGLARLFTVASYGGLSLGVALALVVEVV
                     WSPFGFLLGFFAVLVIARIVAYAARWNSLNPLWRAVDAMINPVLFRIKRFVYKDRIVN
                     YMQGLVTGAVALVGARVLLGMAFGLVIGLLQRF"
BASE COUNT          211 a          352 c          390 g          185 t
ORIGIN      
        1 acagggccgc cgagtaccag ggccttaaag aaagcgaata gcggggcggg gacgcgcgtc
       61 gatggagggc cgctcggcgc gtcagtcgag ctcgatcgct cgcttctgcg ggtccgggct
      121 cggcctgtag atgatggcca catgcccgat cacgcgaacg agctcggccc ccgccttctc
      181 ggccagctcc ctggccagct ccttgcgctc gcccttgaaa tcgacgaaac gtagcttgac
      241 cagctcgtgg tcggtcaggg cgcggtcgag cgcctcgtgg acgccatcga ccgggccggc
      301 cttgccgagg aacacgatgg ggttcagttc attggcgagc gaggacagcc tggcccgttg
      361 tttcgatgtt agcatgcgtt gttcctttta aaaccgctgc aggaggccta tgacgaggcc
      421 gaaggccatc ccgagcagca ccctggcccc cacgagggct acggcaccgg tgacgaggcc
      481 ctgcatatag ttgacgatcc ggtctttgta gacgaagcgt ttgatcctga acaggactgg
      541 gttgatcatg gcgtcgacgg cccgccagag cggattgagg ctgttccatc tggcggcgta
      601 ggcgacgatg cgcgctatga cgagcacggc gaagaagccg agcaggaagc cgaacggcga
      661 ccagacgacc tcgacgacca gggccagggc cacgccgagg ctcagcccgc cgtacgaggc
      721 gacggtgaac agcctggcca ggcccgacag cgcggcgagc gcggctatcg gcgagaaatc
      781 gacgccaccg gagcggagta tcgggaagcg cctccaccag gacaggtagg gctcggtggc
      841 gcgctccacg agttcggcgg gcttaccgag ccgggcgctc ggtagccatg acatgaacac
      901 gcggacgacg cacagcagca tgtagatcga cgtggccgcg gatagcagcc caaggacggt
      961 tctcagcacg atggctccat tcgttccata gtctcagtcg ccctgttcca gccgttgcag
     1021 ctcgagcatg cgctggcgga ggttcagggc ttcctgggcg gtatcgcgat tctccctcgc
     1081 ggggtcgaag ttcgggttga gcctgagcga ctcgttccag tacgaaatgg ccagcggc
//
LOCUS       DAOOLN010000904         1138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_170963_length_1138_cov_2.129271, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000904 DAOOLN010000000
VERSION     DAOOLN010000904.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170963_length_1138_cov_2.129271"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="PLW80_10705"
     CDS             complement(<1..1110)
                     /locus_tag="PLW80_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPB67028.1"
                     /translation="MAPEPTRYARAWRLGIAALAVVAAVGAAIPFSYSLSRGDRADVE
                     VARSATAPAEGFLSEEPAEGLLSEEPASPVEASSFWSLAGVDEEAALDALLARMGPDE
                     RLGQLFMLSYGGEEPTPLLYSWIRRRGLGGVKIFGWNADDTGKLAAAIEAIQNAAENG
                     PLDVPPFVATDQEGGWIRHVRGSTSVTPGNMAIGASGWPSDAYRSAYYIGVELAALGV
                     TMNFAPTVDLATRPESTIIGPRAFSSDPTETGILAAAWARGMADAGVLATAKHYPGQG
                     DTELDSHGILPVIDIDEETLWNRELVPYRMLAAEGVPGVMSGHLAFPRISGSNEPASL
                     SRHFIQELLRERMGYDGLVITDDLMMSGAASSGGLS"
BASE COUNT          160 a          395 c          416 g          167 t
ORIGIN      
        1 ggacaggcct ccggagctgg ccgccccgct catcatcagg tcgtcggtga tcacgaggcc
       61 gtcgtagccc atccgctcgc gcagcagctc ctggatgaag tgtcgggaca gggaggctgg
      121 ctcgttcgag ccggatatcc tcgggaaggc caggtgcccg ctcatcacgc cgggtacgcc
      181 ctcggcggcc agcatgcggt acggcaccag ctcgcgattc cagagcgtct cctcgtcgat
      241 gtcgatgacc ggcaggatgc cgtgggagtc gagctcggtg tcgccctggc cggggtagtg
      301 cttggcggtc gccaggactc ccgcgtcggc catgccccgg gcccaggcgg ccgcgaggat
      361 gccggtctcg gtcgggtcgg acgagaaggc gcgcgggccg atgatggtcg attccgggcg
      421 cgtggccagg tcgaccgtcg gcgcgaaatt catcgtgacg ccgagggccg cgagctcgac
      481 gcctatgtag taggcgctcc ggtaggcgtc gctcggccag ccggaggcgc ctatggccat
      541 gttgccaggc gtcacgctgg tcgaaccgcg cacgtgcctg atccagccgc cctcctggtc
      601 ggtggcgacg aacggcggta cgtcgagggg gccgttctcg gcggcgttct ggatggcctc
      661 gatggcggcg gccaacttgc ccgtgtcgtc ggcgttccag ccgaagatct tgacgccgcc
      721 gagcccgcgc ctgcgtatcc acgagtatag gagcggcgtg ggctcctcgc cgccgtacga
      781 cagcatgaac agctggccga ggcgctcgtc gggccccatg cgcgccagga gggcatcgag
      841 cgccgcctcc tcgtcgacgc cggccagcga ccagaacgac gacgcctcga ccgggctggc
      901 cggctcctcg gacaagagcc cctcggccgg ctcctcggac aggaacccct cggccggggc
      961 cgtcgccgag cgagcgacct cgacgtcggc ccgatctccg cgactcagcg agtacgagaa
     1021 gggtatggcc gcgcccaccg cggcgaccac ggccagcgcc gcgatgccga ggcgccaggc
     1081 tcgagcgtaa cgggtcggtt cgggggccat gccgcttcgg cctagaggta ggccttgc
//
LOCUS       DAOOLN010000905         1138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_170966_length_1138_cov_2.119114, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000905 DAOOLN010000000
VERSION     DAOOLN010000905.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170966_length_1138_cov_2.119114"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="PLW80_10710"
     CDS             complement(<1..779)
                     /locus_tag="PLW80_10710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPB67029.1"
                     /translation="MKFEGSDQVYAYLNRFLNFERKLQPTEYRLDRMTRLKEMFGRPD
                     EGYRVYHVAGSKGKGSTAAMLAAMLSASGRRTGLYTSPHLLSFKERIAIDGEPVGDAT
                     LVACAEELSDRVDAERADGYPGGEEPTYFELLTMLAFLCFRRAGCTDAVFEVGLGGRL
                     DSTNVVRPVVCLITPIELEHTELLGDTIAEIAGEKAGILKPGVPAFTSATKPDALRVI
                     RERARELGCALTVLDEAAELSGVTISERGTEADVRWLAPGAS"
     gene            complement(776..>1138)
                     /locus_tag="PLW80_10715"
     CDS             complement(776..>1138)
                     /locus_tag="PLW80_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67030.1"
                     /translation="RGRDLRFFFARFAGMGDDDEIPPCVSVLREGSGLASNAHAGGSM
                     VPFAAPGDLVAEARRVFVDSGLIYGTVDFLFADETGSSFFVCETNACPGFEALEACTG
                     LDAAGAILRSVSFAGGEP"
BASE COUNT          191 a          383 c          382 g          182 t
ORIGIN      
        1 gacgctcccg gagcgagcca ccgaacgtcc gcctcggtgc ctcgctcgct gatcgtcacg
       61 cctgaaagct cggccgcctc gtcgaggacg gtcagcgcgc agccgagctc ccgggcccgt
      121 tcacgtatga cccggagcgc gtccggcttg gtcgcgctcg tgaacgccgg cacgcccggc
      181 ttgagtatcc cggccttctc gccggctatc tcggctatcg tgtcgccgag cagctcggtg
      241 tgctccagtt ctatcggcgt gatcaggcag acgaccggcc tgaccacgtt tgtcgagtcg
      301 agccggccgc cgaggcctac ctcgaagacg gcgtccgtac agccggctcg cctgaagcac
      361 aggaaggcga gcatcgtcaa cagctcgaaa taggtaggtt cctcgccccc gggatagcca
      421 tcggcccgct ccgcgtcgac cctatccgac aactcctcgg cgcaggctac gagcgtagcg
      481 tcgccgaccg gttctccgtc gatggctatg cgctccttga acgacaggag gtgcggcgag
      541 gtgtagaggc ccgtccgccg gccggaggcg ctcagcatgg cggcgagcat ggccgcggtc
      601 gaacccttgc ccttggagcc ggcgacgtgg tagacccggt agccctcgtc gggccggccg
      661 aacatttcct tgagcctggt catgcgatcg agccggtact cggtcggctg tagcttgcgc
      721 tcgaagttga ggaagcggtt caggtaggcg tagacctggt ccgagccttc gaatttcatg
      781 gttcacctcc ggcgaacgat accgacctga ggatggcgcc ggccgcgtcg agtccggtac
      841 aggcttcgag ggcttcgaag cccgggcagg cgttcgtctc gcagacgaag aacgacgagc
      901 cagtctcgtc ggcgaacagg aagtcgaccg tgccgtagat caggcccgag tcgacgaaga
      961 cgcgcctcgc ctcggccacg agatcgcctg gcgcggcgaa gggtaccatc gaaccgccag
     1021 cgtgggcgtt cgaggcgagg ccggacccct cgcgcagcac cgagacgcaa ggagggatct
     1081 cgtcgtcgtc gcccattccg gcgaaacgcg cgaagaagaa acgtaggtcg cgcccgcg
//
LOCUS       DAOOLN010000906         1137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_171134_length_1137_cov_2.743068, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000906 DAOOLN010000000
VERSION     DAOOLN010000906.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171134_length_1137_cov_2.743068"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..>1137)
                     /locus_tag="PLW80_10720"
     CDS             complement(297..>1137)
                     /locus_tag="PLW80_10720"
                     /inference="COORDINATES: protein motif:HMM:NF017106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P83/100 family protein"
                     /protein_id="HPB67031.1"
                     /translation="PATVAAAEAASQTGAAETGTGTGREADGADGAAEKGGDQEASLA
                     VSEKAREEEAAAAERETSAREAQLEADKAALAAREEASAAKDAEIAADRESIAADQKD
                     AIKGEVSAAAAKDASGVPLFELVDPNLPFSRLALVDLKSGEVIRRSGINTIRAATALD
                     LGDAFVAIAGQVTGSGGAVRLVRVAKADYSKLTQGADDVFADSRLWKIGPSVYAVVKR
                     GELWAIGRFDPVTLELQAVSAPVSRWTFLTESGGRLVAQGPGGAFLVLEAEGLSTAAE
                     IAR"
BASE COUNT          152 a          442 c          355 g          188 t
ORIGIN      
        1 cctgaacccc gccgcgtcgc cgcccgccgc cagctcggcc ttcagctccc gcgccttccg
       61 gtacaggtag cgctgctcgt cgaccgatag gtcccccacc gcgccgatcg accgcccctt
      121 gaatcgcccg ttcctcgtca tacgcactcc ttccatagtg ctacgaacct tatgcatccg
      181 aactgaagcg gctcatgctc cgtgcttatc gaggcgcgct cgacgaagcc ggcgcccacc
      241 ctacccgaac ggcaaaaaaa aacggaaggc atcgctgcct tccgtttcca tcctcgtcac
      301 cgcgctatct ccgcggcggt ggacaagcct tccgcttcca ataccaggaa ggccccgcct
      361 ggcccctggg ccaccaagcg cccgcccgac tccgtcagga aggtccagcg gctgaccggg
      421 gccgaaaccg cctggagctc cagggtaacg gggtcgaaac gccctatcgc ccagagctcg
      481 ccgcgcttga cgacggcgta gacggacggg cctatcttcc agagcctcga gtcggcgaag
      541 acgtcgtccg cgccctgggt cagcttggag tagtcggcct tggcgacgcg gaccagcctg
      601 accgctcctc ccgagccggt cacctggccg gctatcgcga cgaaggcgtc gccgaggtcg
      661 agcgccgtcg cggcgcgtat cgtattgata ccgctacgcc tgatcacctc gccggacttg
      721 aggtcgacga gggccagtcg cgagaacggc aggttcgggt cgacgagctc gaacaggggc
      781 acgccggagg catccttggc cgcggcggcg cttacttcgc ccttgatggc gtccttctga
      841 tcggccgcga tggactcgcg gtcggcggct atctcggcgt ccttggctgc ggaggcctcc
      901 tcgcgggcgg ccagggccgc cttgtcggcc tcgagctggg cctcgcgggc gctcgtctct
      961 cgctcggccg cggccgcctc ctcctcgcga gccttctccg acaccgcgag gctcgcctcc
     1021 tggtcgccgc ccttctctgc ggccccatcg gccccgtcgg cctcgcgccc ggtcccggtc
     1081 ccggtctcgg cggcgccagt ctggctggcc gcctcggccg cggcgaccgt cgctggc
//
LOCUS       DAOOLN010000907         1136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_171358_length_1136_cov_3.223867, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000907 DAOOLN010000000
VERSION     DAOOLN010000907.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171358_length_1136_cov_3.223867"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..724
                     /locus_tag="PLW80_10725"
     CDS             122..724
                     /locus_tag="PLW80_10725"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPB67032.1"
                     /translation="MKRLALVLVAICALPLGADELEDALYRSVDALSQAYRKAHPDLG
                     MRAGLAMLDFEEQSELARRHGLGTALESYVRDTVERSLVYELVDRRRMSDILAELELS
                     LSGLVDGRDAVEAGRIAGVRAFLWGGIAELGDSFVISLGVTDAETGLVLATESFELPK
                     RLLVSAAEELAYSYVAPNGIGLSVHGFAPLFQLSDLYTKS"
     gene            791..>1136
                     /locus_tag="PLW80_10730"
     CDS             791..>1136
                     /locus_tag="PLW80_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67033.1"
                     /translation="MTAPSLTGEHYRVDEDIDVLSVQPELAATVDPWGLAGSTANAGF
                     SQWTARFRATFIRLDAQYTVNFSPRFNIGLAGGIFNALGNVQMYVDMGGDDSGPYYRQ
                     QLAMESADPMADD"
BASE COUNT          184 a          377 c          351 g          224 t
ORIGIN      
        1 cccgtgcttc gctttagggc aatagactat tctttcgtcg tatttgaacg ccccgggccg
       61 gcggcttata gtatgagcgt cttcgtctca ccgtccgcta tagagcgccg aggagggaat
      121 catgaagagg ctcgcccttg tccttgtagc tatctgcgcc ttgccgctcg gggccgacga
      181 gctggaggac gccctgtacc gcagcgtcga cgcgctttcc caggcttata ggaaggcgca
      241 tccggacctc ggcatgaggg ccggccttgc catgctcgat ttcgaggagc agtctgagct
      301 cgccaggcgc cacggcctcg gtacggcgct cgagagctat gtgcgcgaca cggtcgagcg
      361 ctccctggtc tacgagctcg tcgaccgcag acggatgtcc gatatcctcg cggagctgga
      421 gctgagcctc tccggcctgg tcgacggccg cgacgccgtc gaggctggcc ggatagccgg
      481 cgtacgcgcc ttcctctggg gcggcatcgc cgagctgggc gactcgttcg tcatcagcct
      541 cggggtgacc gacgcggaga ccggcctggt cctggcgacc gaatccttcg aacttccgaa
      601 gcgcctgctt gtctccgcgg ccgaggagct cgcgtactcg tacgtcgcgc cgaacggcat
      661 cggcttgtcc gtccatggct tcgcgccgct gttccagctg tcggacctct ataccaaatc
      721 gtagctcttc ttcgccgatc tcggcttttc ctaccggccg aaccgccact tcatggtgtc
      781 gggcggcatc atgaccgccc cctcgctgac cggggagcac tacagggtcg acgaggatat
      841 cgacgtgctg tccgtccagc cggagctggc cgcgacggta gacccatggg gattggccgg
      901 ctccaccgcc aatgccggct tcagccaatg gaccgcccgg ttcagggcca ccttcatcag
      961 gcttgacgcc caatacacgg tcaacttcag cccacgcttc aatattggtt tggccggcgg
     1021 aatcttcaac gcgctcggca atgtacagat gtacgtcgat atgggcggcg acgactcggg
     1081 gccgtactac agacagcaac tggccatgga atccgccgat ccgatggccg acgact
//
LOCUS       DAOOLN010000908         1135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_171667_length_1135_cov_2.519444, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000908 DAOOLN010000000
VERSION     DAOOLN010000908.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171667_length_1135_cov_2.519444"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1135)
                     /locus_tag="PLW80_10735"
     CDS             complement(<1..>1135)
                     /locus_tag="PLW80_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HPB67034.1"
                     /translation="IEAEYNSGAISEEEARRRKDDVQREVDFYGQMDGASKFVAGNVT
                     VGIFISVINLIGGFIVGMVLHGEPFASALNTYTSLTIGDGLVSQLPALLISVSTGIIV
                     TRANSQGTFGSEVTAQFTQNARIYWVGAAVLSALALLPGFPWYVLLPMAAAMAYLAWN
                     LSKKAVLESDKAKLRAAEGGAKKPESSQELSPVVPLDPISLELGYGLIPLVDKDKGAE
                     LLERVTRIRKESAIDMGLVVPRIRIIDNMRLEPSEYCFKIRGVEMGRGVIRLGYYLAI
                     NPGGVSEEVQGERTKDPAFGLPAVWISEDARDRAERAGYTVVDAPSIVATHLTEIIKR
                     HAADILGRQEIQGMLDALKKDYPAVVEDAQKHLSLGEIQKVLQG"
BASE COUNT          176 a          368 c          381 g          210 t
ORIGIN      
        1 ggccctgaag caccttctgg atctcgccga gcgacaggtg cttctgcgcg tcctcgacga
       61 ccgccggata gtcttttttg agggcgtcga gcatgccctg gatctcctgc cgcccgagga
      121 tatcggcggc gtggcgcttg atgatctcgg tcaggtgagt cgcgacgatg ctcggcgcgt
      181 cgacgaccgt gtagcccgcc cgctcggccc gatcgcgcgc gtcttcagat atccagaccg
      241 cgggcagccc gaaggcgggg tccttggtgc gctcgccctg aacctcctcg gacacgccgc
      301 cggggttgat cgccaggtag tagccgaggc gtatgacgcc gcgccccatc tcgacgccgc
      361 gtatcttgaa gcagtactcc gacggctcga gccgcatgtt gtcgatgatg cggatacgcg
      421 ggacgacgag gcccatgtct atggcgctct ccttgcgtat cctcgtgacg cgctcgagca
      481 gctccgcgcc cttgtccttg tcgacgagcg gtatgaggcc gtagccgagc tcgaggctga
      541 tcgggtcgag cggcacgacc ggcgacagct cctgggacga ctcgggcttc ttggcgccgc
      601 cctcggcggc cctcagcttg gccttgtcgg actcgaggac agccttcttc gagaggttcc
      661 aggccaggta cgccatagcg gccgccatcg gcaggagtac gtaccacggg aagccgggca
      721 ggagcgcgag ggcgctcagc accgccgcgc ccacccagta gatcctggcg ttctgcgtga
      781 actgcgcggt gacctcggag ccgaaggtgc cttggctatt ggcccgcgtg acgatgatgc
      841 cggtggagac ggagatgagc agcgcaggca gctgggagac gagcccgtcg cctatcgtca
      901 gcgaggtata ggtgttcagt gccgaggcga acggctcgcc atggagcacc atgccgacga
      961 tgaagccgcc tatcaggttg atcacgctga tgaagatgcc gacggtcacg ttgcccgcga
     1021 cgaacttgct ggcaccgtcc atctggccgt agaagtcgac ctcgcgctgg acgtcgtcct
     1081 tgcggcgcct ggcctcttcc tcgctgatgg cgcccgagtt gtattctgcc tcgat
//
LOCUS       DAOOLN010000909         1134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_171849_length_1134_cov_3.008341, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000909 DAOOLN010000000
VERSION     DAOOLN010000909.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171849_length_1134_cov_3.008341"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLW80_10740"
     CDS             complement(<1..322)
                     /locus_tag="PLW80_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67035.1"
                     /translation="MERTSARSPQAPALLLISGLGHGLALAYASLMPRAAEPHAGPAY
                     FIILIAASAVLVVPLARWAGDPAAAALALAAKLGLAILALIPIVAPSPLKTLILSVLV
                     AEAVL"
     gene            complement(406..>1134)
                     /locus_tag="PLW80_10745"
     CDS             complement(406..>1134)
                     /locus_tag="PLW80_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HPB67036.1"
                     /translation="FSPYYDAFYGIHRYADRAEVIRMAAVEAARGAVRIRDELGIPIV
                     GFDIAGQEAGHPAGQFREAFDFVHQNFMHKTVHAGEAYGAESIFQAITDLHADRLGHG
                     YYLFDPDKVAAASVRDKRRYIERLAGFVADQRVTIEVCLTSNLQTNPSLERVADHSFG
                     KMLERRLSATLCTDNRLVSNTTVSKEIRLAVDNFDIGPSALKNLIVYGFKRSFYPGPY
                     AAKREYVRRAITRYEAVARKYGVE"
BASE COUNT          165 a          360 c          424 g          185 t
ORIGIN      
        1 cgaggaccgc ctccgcgacc aggaccgaca ggatgagcgt cttgagcggg ctcggggcga
       61 cgatcgggat cagcgcgagt atcgccaggc cgagcttggc ggcgagcgcc agggccgcgg
      121 ccgcgggatc gcccgcccag cgggcgagcg gcaccaccag gaccgcgctc gcggcgatta
      181 agattatgaa atacgccggt ccggcgtgcg gctccgccgc cctcggcatc aggctcgcgt
      241 aggcgagggc caagccgtgt ccgaggcccg agatgaggag cagggcgggg gcctgcggcg
      301 atcgtgctga cgtgcgctcc atcggtggac gatagtctgt agccgtagcg cggttctgtc
      361 aacgctcgga gcgggccggt gccgcccgcc tcgggccgtc cacgctcact cgacgccgta
      421 tttcctggcg accgcttcgt agcgggtgat cgcgcgccgt acgtactcgc gcttggcggc
      481 gtaggggccg gggtagaagc tgcgcttgaa gccgtagact atcaggttct tgagggcgga
      541 cgggccgatg tcgaagttgt cgacggccag cctgatctcc ttggagacgg tcgtgttgga
      601 taccagccgg ttgtcggtgc acagggtcgc cgacagcctg cgctcgagca tcttgccgaa
      661 ggagtggtcg gcgacgcgct cgagggacgg gttcgtctgc aggttgctcg tcaggcagac
      721 ctcgatggtc acgcgctggt cggccacgaa gccggccagc ctctcgatgt agcggcgctt
      781 gtcccgcacg ctcgccgcgg cgaccttatc cgggtcgaag aggtagtagc cgtgcccgag
      841 ccggtcggcg tgcaggtcgg tgatagcctg gaagatgctc tcggcgccgt aggcctcgcc
      901 ggcgtggacg gtcttgtgca tgaagttctg gtggacgaag tcgaaggcct cgcggaactg
      961 gccggccggg tggcccgcct cctggcccgc gatgtcgaac ccgacgatgg ggatcccgag
     1021 ctcgtcgcgt atcctgaccg cgccgcgggc cgcctcgacc gcggccatcc gtatcacctc
     1081 ggcccggtcg gcgtagcggt ggatcccgta gaacgcgtcg tagtacgggg agaa
//
LOCUS       DAOOLN010000910         1134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_171900_length_1134_cov_2.483781, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000910 DAOOLN010000000
VERSION     DAOOLN010000910.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171900_length_1134_cov_2.483781"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1080
                     /locus_tag="PLW80_10750"
     CDS             163..1080
                     /locus_tag="PLW80_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5692 family protein"
                     /protein_id="HPB67037.1"
                     /translation="MFLYQSIPWYSLLMWLAVFAGLVLLNELGRSSKWAGLALFVILP
                     LALTVTPWPKSAGPGTSMDDWFHWAKVYSALAGCIGFMAIRFIPKVAKHRYALAFPAL
                     ILAVNILEAVIRDFECRSFNGVVGGILTIGGPWNVMNGIAGILNIVTITGWMGIFAGK
                     GKSRDMLWPDMLWFWIIAYDLWNFAYTYNCLGDHSFYCGLALLLSCTFAAFFIKKGAW
                     LQHRAQTLAFWCMFAMSAPWFIDDSIFAVKASGNPKALFLVSLVALVANVAVFAYWLY
                     RILKTRRNPLKAELFTDLKAYKGVAAERA"
BASE COUNT          199 a          384 c          311 g          240 t
ORIGIN      
        1 tgcacgccat tatcctatac atgaataatg actatattgt ctatataaac cttgttgtcg
       61 ataaacttga cactatatta caatatctgt acaactatcg cgatcacggc gtcgccgcgg
      121 ctcaacgata cgacgcgcct ccgcgcgttc gaggatgtat ccatgttcct ataccaatcg
      181 atcccatggt attcgctgct catgtggctg gcggtcttcg ccggcctcgt cctgctcaac
      241 gagctcggcc gatcgagcaa gtgggccggc ctggccctct tcgtcatcct tcctctggcg
      301 ctcaccgtta ccccctggcc gaaatccgcc ggcccgggca cgagcatgga cgattggttc
      361 cactgggcca aggtctactc ggcgctcgcc ggctgcatcg gcttcatggc catacgcttc
      421 atacccaagg tggccaagca ccgctacgcc ctggcctttc cggcgctgat actagccgtc
      481 aacatcctcg aggcggtcat acgcgatttc gagtgccgct cgttcaacgg cgtcgtcggc
      541 ggcatcctga ccataggcgg cccctggaac gtcatgaacg gcatcgccgg tatcctgaac
      601 atcgtcacca tcaccggctg gatgggcatc ttcgccggca agggcaagtc gcgagacatg
      661 ctctggccgg acatgctctg gttctggatc atcgcctacg acctgtggaa cttcgcctac
      721 acctataact gcctcggcga ccactcgttc tactgcgggc tggccctgct cctgtcctgc
      781 accttcgcgg ccttcttcat caagaagggc gcctggctcc agcaccgcgc ccagaccctc
      841 gcgttctggt gcatgttcgc gatgagcgcg ccgtggttca tagacgactc gatcttcgcg
      901 gtcaaggcct cgggaaaccc gaaggcgctg ttcctggtca gcctcgtggc gctcgtagcc
      961 aacgtcgcgg tcttcgccta ctggctgtac aggatcctga agacgaggcg caaccccctc
     1021 aaggccgagc ttttcaccga cctcaaggcc tacaagggcg tggcggccga gcgcgcctga
     1081 cgagaggcgc ggaatcgctc gcggcggcca gggcccggcc gccgccgtta cgct
//
LOCUS       DAOOLN010000911         1133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_172114_length_1133_cov_2.705937, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000911 DAOOLN010000000
VERSION     DAOOLN010000911.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172114_length_1133_cov_2.705937"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLW80_10755"
     CDS             <1..204
                     /locus_tag="PLW80_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67038.1"
                     /translation="ILEHKGSVKLDGFMRRIDEILISVYDGFQSGGDRVDTGYRGRPA
                     AFRRGDPALAVIALAAVVSLLIR"
     gene            283..>1133
                     /locus_tag="PLW80_10760"
     CDS             283..>1133
                     /locus_tag="PLW80_10760"
                     /inference="COORDINATES: protein motif:HMM:NF019974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate- and nitrite sensing domain-containing
                     protein"
                     /protein_id="HPB67039.1"
                     /translation="MLVLIAVPLAMVAAMGAMLILSSMGDKGEIDDAESNLTIMKSLS
                     SLLSAAQDERGIAYLYLSGTAAADRIMSTRNGTGSSWETVQAALAAVPAPESLETVLT
                     SSVARFQQLRRQVDEHSMMAQSAYTGYSELIDLVMAEYVALSATNAAEASRQLQSIVY
                     LEKAKENAARIQCLASSIFSLDLAIQYTKTIELVDTNAGIVVNLANAGEYCGELASVS
                     LSELEISPEWYELQDAVLSILGNAQYGGYGRDGLAFYNATEPVMDKIQATIDIVMAET
                     DAWFAERS"
BASE COUNT          210 a          357 c          359 g          207 t
ORIGIN      
        1 atcctcgagc acaagggctc ggtcaagctc gacggcttta tgcggaggat cgacgagata
       61 ctgatttcgg tctatgatgg gttccagtcc ggtggcgatc gagtcgatac tggatacagg
      121 ggacgtccgg ccgcctttag gcgcggtgac ccggccctgg ccgtcatcgc gctcgccgcg
      181 gtggtttcgt tgctcatcag gtaaaaagcc ggcccgatag ctcagcgggc aacatccagg
      241 tataccgagg ttcgtagtat ggctctttct ctgagaagca agttgctcgt actcattgcg
      301 gttccgctgg ccatggtcgc ggcgatgggc gcgatgctca tactatcgtc gatgggcgac
      361 aagggcgaga tcgacgacgc cgagtcgaac ctgaccatca tgaagtcgct gagctccctg
      421 ttgtcggcgg ctcaggacga gcgcggcatc gcgtacctct acctttcggg caccgccgcc
      481 gcggatagga tcatgtcgac gaggaacggc acgggaagct cctgggaaac ggtccaggcg
      541 gcgctcgccg ccgttccagc accggaaagc ctcgagacgg tcctgacgtc gtccgtggcg
      601 cgtttccagc agctgcgcag gcaggtcgac gagcactcga tgatggccca gtcggcctac
      661 accggctact cggagctcat cgacctggtc atggcggagt acgtggcgct ttcggccacc
      721 aatgccgcgg aggcctcccg gcagctccag tccatcgtct acctcgagaa ggccaaggag
      781 aacgccgccc gtatccagtg cctggccagc tccatcttct cgctcgacct ggccatccag
      841 tacaccaaga ccatcgagct ggtcgacacg aacgcaggta tcgtggtgaa cctggccaac
      901 gccggggagt actgcggcga gctggcctcc gtgtccctat cggagctgga gatcagcccc
      961 gagtggtacg aactccagga cgccgtgctg tccatcctcg gtaacgccca gtatggcggc
     1021 tacggccgcg acggcctcgc gttctacaac gcgaccgagc cggtcatgga taagatccag
     1081 gcgaccatag acatcgtgat ggccgagacg gacgcctggt tcgccgagcg ctc
//
LOCUS       DAOOLN010000912         1133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_172158_length_1133_cov_2.422078, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000912 DAOOLN010000000
VERSION     DAOOLN010000912.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172158_length_1133_cov_2.422078"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="PLW80_10765"
     CDS             complement(<1..1073)
                     /locus_tag="PLW80_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HPB67040.1"
                     /translation="MASLGINIGSSSLKLVLAEGDTIVWSASTPHEGDFHSAVRKLLS
                     EKAIPLGTKVLVTGNEGRYLFNAAGTLEPLCVEAALKASGLKADAVASMGGEDLVVYS
                     LDQNGKIVNNFSGNKCASGTGEFLKQQLARMDMTLADIAKVPDSARVLALSTRCSVFM
                     KSDCTHRLNKREATKDDIVLSLSNVMAGKVVDFLKRAKITDGRVILTGGLTLNKHMLR
                     FIKERAPGIDFIVPDSASVFEAYGAAVLAESAGSPLPSTESLLKPNVVRFDTLPDLKK
                     WRDKVRFFEKGEGKVVAGRKYVLGVDGGSTTTKACLVDAETDEIVASHYGRTHGDPVK
                     ALKECLAVIRDKIIADTGDAKVD"
BASE COUNT          182 a          352 c          377 g          222 t
ORIGIN      
        1 atgtcgacct tggcgtcgcc cgtatcggcg atgatcttgt cgcggatgac ggcgaggcac
       61 tccttgaggg ccttgaccgg gtcgccgtgg gtgcggccgt agtggctcgc gacgatctcg
      121 tcggtctccg cgtcgacgag gcaggctttc gtcgtcgtcg agccgccgtc cacgccgagt
      181 acgtacttcc tgccggcgac gaccttgccc tcgcccttct cgaagaagcg caccttgtcg
      241 cgccacttct tgaggtcggg gagggtgtcg aagcgcacca cgttgggctt gagcagggac
      301 tcggtcgacg gcagcgggga gccggcgctc tcggcgagca ccgcggcgcc gtaggcctcg
      361 aagaccgagg cgctatcagg cacgatgaag tcgataccgg gcgcccgctc cttgatgaag
      421 cgcagcatgt gcttgttcag cgtcaggccg ccggtcagga tgaccctgcc gtcggtgatc
      481 tttgcccgct tgaggaagtc gacgaccttg ccggccatca cgttcgacag cgacaacacg
      541 atgtcgtcct tggtggcctc gcgcttgttc agcctgtggg tgcagtcgct cttcatgaac
      601 accgagcagc gcgtggacag cgcgagaacc ctggcgctat ctggcacctt cgcgatgtcg
      661 gcgagcgtca tgtccatgcg ggccagctgc tgcttgagaa actcgccggt gcccgaggcg
      721 catttattgc ccgagaagtt gttgactatc ttgccgttct ggtcgagcga gtagacgacg
      781 aggtcctcgc cgcccatgct cgcgacggcg tccgccttga gacccgacgc cttgagcgcg
      841 gcctcgacgc agagcggctc gagggtgccg gcggcgttga acaggtagcg gccttcgttg
      901 ccggttacca ggactttcgt cccgaggggg atggccttct cggaaagcag tttccgaacg
      961 gcggaatgga aatcgccctc gtggggcgtc gacgccgacc agactatcgt atcgccttcg
     1021 gcgagcacga gcttgagact cgaggagcca atgttgatgc ctaatgatgc catagacgag
     1081 aacctcaccg ggacttttgt cgcagatttc caggcgccac gccgtgtccg gga
//
LOCUS       DAOOLN010000913         1132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_172335_length_1132_cov_3.755803, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000913 DAOOLN010000000
VERSION     DAOOLN010000913.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172335_length_1132_cov_3.755803"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="PLW80_10770"
     CDS             complement(<1..802)
                     /locus_tag="PLW80_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67041.1"
                     /translation="MRSFARVVAIALSLSIQSCAGDSIHGLDEAGWFELLAKGDPLPI
                     AYGDRVDYDAIARLGPGASLFVGLRALEAGDEKLAAGLFRDAAARETGRYKARAVASL
                     VETLQSTGDGEALVELCRSELGSELAPYRRAYLEAVGYAVSGSHAHTLASVDALRSAY
                     PAESSRDAAAIASMALEAGYRSGKGRWVNDFIALTAMEGSAAIYEALAKATSLVASSG
                     RAEAEAAIRAVGVRAFQLAEARALTGSRNYGPAVVAFRRYAADREDPAD"
     gene            complement(944..>1132)
                     /locus_tag="PLW80_10775"
     CDS             complement(944..>1132)
                     /locus_tag="PLW80_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPB67042.1"
                     /translation="PTPAAPAKKEGTWHKIKWGDTLWDISIAYYRTPWLYMTIAKANK
                     IKNPDLIISGTWIYIPPR"
BASE COUNT          179 a          371 c          388 g          194 t
ORIGIN      
        1 cgtcggccgg gtcttcccgg tcggcggcgt agcgcctgaa ggcgacgaca gccggcccgt
       61 agttccgtga accggtcagc gcgcgggcct cggccagttg gaaggccctg accccgacgg
      121 cccgtatcgc ggcctccgct tcggctcggc cggatgaggc cacgaggctc gtggccttgg
      181 cgagcgcctc gtagatggcc gccgatcctt ccatcgccgt gagcgctatg aagtcgttca
      241 cccagcggcc cttgccggag cggtagcccg cctcgagggc catcgacgcg atggccgccg
      301 cgtcccgcga ggattcggcg gggtaggccg agcggagcgc gtccacactc gccagggtat
      361 gggcatgcga gcccgagact gcataaccga cggcctccag gtaggccctt cggtacggcg
      421 cgagttccga gcccagctcg gagcggcaca gctccaccag cgcttcccca tcgcctgtcg
      481 attgcagggt ttcgacgaga gaggcgaccg cccgcgcctt gtatcgcccg gtctctcgcg
      541 ccgcggcatc cctgaacagc ccggcggcga gcttctcgtc gcccgcttcg agggcccgca
      601 ggccgacgaa caagctggct ccgggcccga gcctggcgat ggcgtcgtag tcgacgcggt
      661 cgccgtaggc gatgggcagg gggtcgcctt tcgctaagag ctcgaaccag cccgcctcgt
      721 cgaggccgtg gatcgagtcg ccggcgcatg actggatgga taggctcagg gctatggcga
      781 cgacgcgggc gaatgatcgc atggactctc ctggcgaggt gatggtggcc cgttggcgcg
      841 ggcctcgcta ctactattcc acgaaagcgg ccataggcca agcgtcgagc cttaaaaaaa
      901 acggcggcgc agatgcgacc gccgttatcg cccaggctcg cgcctagcgc ggcgggatgt
      961 agatccaggt tcccgaaatg atcaggtcgg ggttcttgat cttattggcc ttggctatcg
     1021 tcatgtacag ccatggagtt cggtagtagg cgatcgaaat atcccagagc gtgtcgcccc
     1081 acttgatctt atgccaagta ccctctttct tagccggggc ggcgggagta gg
//
LOCUS       DAOOLN010000914         1131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_172639_length_1131_cov_2.713755, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000914 DAOOLN010000000
VERSION     DAOOLN010000914.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172639_length_1131_cov_2.713755"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PLW80_10780"
     CDS             complement(<1..549)
                     /locus_tag="PLW80_10780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HPB67043.1"
                     /translation="MTVRAALAEGSSALEGSGSGSPFLDAALLLGHTLGVGRDRVLAS
                     MPDELGASALEAYQAFVARRSSGEPIAYIVGYKEFYGRRYTVDPRVLVPRPDTELLVE
                     VALSLLPPVSRTRGGSARVRCHDAFTGSGCIGVTLAAERPDVEVSLSDASPAALEVAR
                     ANARELLGRELDARLGDVLSAAV"
     gene            complement(572..>1131)
                     /locus_tag="PLW80_10785"
     CDS             complement(572..>1131)
                     /locus_tag="PLW80_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HPB67044.1"
                     /translation="GKLRWESGVHRVQRVPATEGSGRIHTSAVTVAVLPEVEETEVEI
                     RPEDLRVDVMRAGGPGGQCVNTTDSAVRLTHIPTGLVVHCQDEKSQIKNKAKAMRILR
                     ARIYELEEEKKNAERAAERRGQIGSGDRSERIRTYNFPQNRVTDHRIDVTLYKLDLFM
                     QGDMDEIVQGLAIRAREDAMKAEGQ"
BASE COUNT          170 a          386 c          387 g          188 t
ORIGIN      
        1 gacggcggca gacagcacgt cgccgaggcg cgcgtcgagc tcgcggccga gcagctcgcg
       61 ggcattggcg cgcgccacct cgagggcggc cggggacgcg tccgatagcg agacctcgac
      121 atcgggacgc tcggccgcca gcgtcacgcc gatgcagcca gagccggtaa aggcgtcgtg
      181 gcaccgtacc cgggccgatc ctccgcgcgt tcgcgatacc ggcgggagta gcgacagagc
      241 tacctccacg agcagctcgg tatccgggcg cggtaccaat acgcgcgggt cgacggtata
      301 tcgtcgtcca tagaattcct tatacccgac gatatacgcg ataggttcgc cggaggacct
      361 gcgggccacg aaggcctggt acgcctcgag cgccgaggcg ccgagctcat ccggcatgga
      421 ggcgaggacc ctatcgcgcc ccacaccgag tgtgtgaccg aggagtaggg cggcgtccag
      481 gaaaggcgat cccgagccgc tcccctcgag ggcgctggat ccttccgcga gcgccgcccg
      541 cacggtcacg gggtcggctc cgatcggggg atcactgccc ctccgccttc atcgcgtcct
      601 cgcgggcgcg gatggcgagg ccttgcacga tctcgtccat gtcgccttgc atgaacaggt
      661 ccagcttgta cagcgtcacg tcgatacgat ggtcggtgac gcggttctgc gggaagttat
      721 aggtacggat gcgctcggaa cgatcgcccg agcctatctg gccgcggcgc tcggcggccc
      781 gctccgcgtt cttcttctct tcctcgagct cgtagatgcg cgcgcgcagg atgcgcatcg
      841 ccttggcctt gttcttgatc tggctcttct cgtcctggca gtggacgacg aggccggtcg
      901 gtatatgggt cagccggacc gccgagtcgg tcgtgttgac gcactgtccg ccagggcctc
      961 cggcgcgcat cacgtcgacg cgcaggtctt cgggccggat ctcgacctcg gtctcctcga
     1021 cctcgggcag caccgcgacc gtaacggcgc tcgtatggat gcggccggag ccctcggtgg
     1081 ccggtacgcg ttgcacgcgg tgcacgcccg actcccagcg aagcttgccg a
//
LOCUS       DAOOLN010000915         1130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_172944_length_1130_cov_2.260465, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000915 DAOOLN010000000
VERSION     DAOOLN010000915.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172944_length_1130_cov_2.260465"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PLW80_10790"
     CDS             complement(<1..364)
                     /locus_tag="PLW80_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67045.1"
                     /translation="MARIVGIEKGASGAVKAVAVDGSFFIFRNVFLAAALMERGLSLP
                     FTERRDDDDPFVEVADEALDDAVAATEAEARALALLARAEQYRFGLERKLTARGFSRF
                     AIRVALDGRGIPDDGHRID"
     gene            complement(388..780)
                     /gene="rpsI"
                     /locus_tag="PLW80_10795"
     CDS             complement(388..780)
                     /gene="rpsI"
                     /locus_tag="PLW80_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPB67046.1"
                     /translation="MVRNLGIGTGRRKTAVARVYLREGTGKITVNNLELEKYFPIAEI
                     AGSVRQPLVVTASDNKFDIVINVVGGGPNGQAGACRHGIARALSQVDPASSIALRNNG
                     FLTRDPRMVERKKYGQRGARRRFQFSKR"
     gene            complement(793..1092)
                     /gene="rplM"
                     /locus_tag="PLW80_10800"
                     /pseudo
     CDS             complement(793..1092)
                     /gene="rplM"
                     /locus_tag="PLW80_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694886.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
BASE COUNT          204 a          352 c          346 g          228 t
ORIGIN      
        1 ggtctatccg gtgaccgtcg tctgggatcc cccggccgtc gagggcgacc cggatcgcga
       61 accgagagaa gcctcgagcg gtcagtttcc gctccagacc gaaacggtac tgctccgccc
      121 ttgccaacag agccagtgcc ctagcctcgg cttccgtcgc cgcgacggcg tcatcgagcg
      181 cttcgtccgc cacctcgacg aaagggtcgt cgtcatcccg gcgctcggtg aacggcaagg
      241 aaaggccgcg ttccatgagc gccgcagcca gaaagacatt ccggaaaata aaaaaggagc
      301 catccacggc gaccgccttg acggcgccgg aggctccctt ctcgataccg acgatcctgg
      361 ccatcaggcc gtttcgtcca tcgtgtctta gcgcttggag aactggaagc gcctgcgcgc
      421 tccacgctgt ccgtacttct tgcgctcgac catacgcgga tcgcgggtca ggaaaccgtt
      481 attcctgagc gcgatgctcg atgcggggtc gacctggctg agggcgcggg cgatgccgtg
      541 cctgcaggcg ccggcctggc cgttgggacc gccgccgacc acgttaatga cgatatcgaa
      601 cttgttgtcg ctggcagtca cgacgagagg ctgacggacg gaaccggcta tctcggctat
      661 cgggaagtac ttctcgagct cgaggttgtt aacggtgatc ttgcccgtgc cctcgcgtag
      721 atagactcgg gccacggcag tcttacgtct accggtacct attccaagat tcctaaccat
      781 cgtttacctc tcctagactt cgatagcgac gggcgactgc gccgcgtgcg ggtggttcgg
      841 ccccgcatag accttcacgt tcatcgcgag cttgcggccg agcggcccct tggggagcat
      901 accgcgaatc gcgatctcga ggggatccgt gggatgccgc tcgatcaggg acgcgaaggt
      961 atttttcttg agcccgccgg catagccggt atggcgataa tacaccttat cgttgaactt
     1021 gttgccggtt acctcgatct tgtcagcgtt gacgatcacg acatagtcgc cgacttcctg
     1081 atgcggggtg tagcaggaag tcggcgacta tgtcgtgatc gtcaacgctg
//
LOCUS       DAOOLN010000916         1130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_172951_length_1130_cov_2.208372, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000916 DAOOLN010000000
VERSION     DAOOLN010000916.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172951_length_1130_cov_2.208372"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..572)
                     /locus_tag="PLW80_10805"
     CDS             complement(99..572)
                     /locus_tag="PLW80_10805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmO family methyltransferase"
                     /protein_id="HPB67047.1"
                     /translation="MKEMTVRAIGRVRADDAKGYYALEVDEPYREGLLALGEFGRLNA
                     LWWAHGMDTLEFRGTLSCELPYAPGVRAGVFACRSEYRPNPIALTSCGIGSVDVDTGL
                     ITLDYIDAVDGTPLLDIKPYIPVCDRARDVRVAPWFREWPDCIEDSAAFFAKFEL"
     gene            794..>1130
                     /locus_tag="PLW80_10810"
     CDS             794..>1130
                     /locus_tag="PLW80_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HPB67048.1"
                     /translation="MEHRLERRRPFVLVGMNFFGNPFGAAAAWDVDNEIGSLWKRLMA
                     YLADHPVAALPPGDPAWFEAHIMHPASAATGDYEVFIGAEAERPLDAPVELVVKSLPA
                     RDYAVLSMRC"
BASE COUNT          188 a          408 c          364 g          170 t
ORIGIN      
        1 ctaccaaggc gacggcgagg tgatcatacc cggcgcgtaa cgcgcccaac gcgcacggcg
       61 cgggagcctc ggaggccccc gcgccgaacg attcgccgct agagctcgaa cttcgcgaaa
      121 aacgccgcgg agtcctcgat gcagtcaggc cactccctga accatggcgc cacacggaca
      181 tccctggcgc ggtcgcaaac cggtatgtag ggcttgatgt cgaggagcgg cgtaccatcc
      241 acggcgtcga tgtagtcgag cgttatcagg cccgtatcga cgtcgaccga cccgatcccg
      301 cagctcgtca gcgctatcgg gttgggccgg tactccgaac ggcacgcgaa cactccggcc
      361 ctgacgcccg gcgcgtacgg cagctcacat gacagcgtgc cgcggaactc tagcgtatcc
      421 atgccgtgcg cccaccatag cgcgttcagg cgcccgaact cccccagggc caggagtccc
      481 tcgcgatacg gctcgtccac ctcgagcgcg tagtagccct tcgcgtcgtc cgcccgcacc
      541 cggccgatcg cccgtaccgt catctccttc atcgtcgcct ccataaggtg tcgtgatggc
      601 ctcagcgtag cgcctcggcg gcgcgcgcct ctcgacctcg attgccggac gccgcggcgc
      661 gaggacgaca ccaggccgag ctccagaggg acagaggtcg aaaccgaccg gaattgccct
      721 atcagtagcc ggggagcgcc cgcggccttg ccaggcgggc actccggcgc tatcgtcgcg
      781 tatggaggcc gggatggaac acaggctcga gcgccggagg cccttcgtac tcgtcggcat
      841 gaatttcttc ggcaacccgt tcggcgcggc cgccgcctgg gacgtggaca acgagatagg
      901 ctcgctctgg aagcgcctga tggcctacct ggccgaccat cccgtggccg ccctgccgcc
      961 gggcgatccg gcctggttcg aggcccacat catgcatccg gccagcgcgg cgaccggcga
     1021 ctacgaggtg ttcataggcg ccgaggcgga gcggccgctc gacgccccgg tcgagctcgt
     1081 ggtcaagtcg ttgccagcca gagactacgc cgtgctctcg atgcgctgcg
//
LOCUS       DAOOLN010000917         1130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_172971_length_1130_cov_2.057674, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000917 DAOOLN010000000
VERSION     DAOOLN010000917.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172971_length_1130_cov_2.057674"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="PLW80_10815"
     CDS             complement(<1..831)
                     /locus_tag="PLW80_10815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPB67049.1"
                     /translation="MDRTRIIKRAAVVSLVGNAALAVLKVAAGFAAGSLAVVGDGVDS
                     STDVLISIVALVAAAVSSKPSDKEHPYGHARAETTATTVLAFFIFFAGAQLILATVRS
                     LLAGEARSVPGPFALWVTAGSIVGKLFLSWTQARAGRRTGSLMLAANAKNMRNDVVIS
                     SSVLAGLGLSILFEAPLADSVVALLVGAWVMRSAVGVFIEANDEIMDGKVDPELYRKV
                     FDAVRSVPGAGNPHRARVRRLASSYDIDLDIEIDGSTSVRKAHEIAQLVEKAIKDRID
                     G"
BASE COUNT          220 a          341 c          391 g          178 t
ORIGIN      
        1 gccgtcgatg cggtccttga tcgccttctc cacgagctgg gcgatctcgt gggccttgcg
       61 gaccgacgtg ctgccgtcta tctctatgtc gaggtcgatg tcatagctcg acgcgaggcg
      121 acgcacccgg gcacggtggg gattgcccgc ccccggcacg ctccgtaccg catcgaagac
      181 ctttcggtac agttccggat cgaccttgcc gtccatgatc tcgtcgtttg cctctatgaa
      241 gacgccgacg gccgaacgca tcacccaggc gccgaccagc agggcgacga cggagtctgc
      301 cagcggcgcc tcgaacagga tggacagccc gaggcctgcg aggacgctcg aggagatgac
      361 cacgtcgttg cgcatgttct tggcgttggc cgcgagcatc aaggagccgg tccgcctgcc
      421 agcgcgggcc tgggtccagg acaggaagag ctttcccact atcgagccgg ccgtaaccca
      481 tagggcgaag ggcccgggca cggaccgggc ttcgccggcc agcagcgagc gcaccgtagc
      541 gaggatgagc tgggccccgg cgaagaagat gaagaaggcg aggaccgtgg tggccgtggt
      601 ctcggccctg gcgtgtccgt aggggtgctc tttgtcggac ggtttggagc tgacggcggc
      661 cgcgaccagg gcgacgatag agatcagcac gtcggtcgac gaatcgacgc cgtcgcccac
      721 gacggcgagc gagccggccg cgaagccggc cgcgacctta agcacagcca gcgccgcgtt
      781 accgacgagg ctgacgacgg cggcgcgttt gatgatccgg gttctatcca tcgggcgagt
      841 ataggccgcg gcgtcgcggc gggcaagaac cttccatgga agcgttactt ccttgctaaa
      901 tgcgttacct taggctagga tgcacgctcg cccgacgccg ggcgcaggga gccgtagatg
      961 acatataaac ttgacggagc aaaattccag acgctagatg agctcgtcga ggcgctgtat
     1021 ccgatgtacg ccgacgagat gtcggaagag gaattccgga agtatgccga agagcatgcc
     1081 gagaagggat aggacgaaga ggcgatgaac gacaggaccg acgacgcgcg
//
LOCUS       DAOOLN010000918         1130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_173004_length_1130_cov_1.729302, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000918 DAOOLN010000000
VERSION     DAOOLN010000918.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173004_length_1130_cov_1.729302"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PLW80_10820"
     CDS             <1..820
                     /locus_tag="PLW80_10820"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPB67050.1"
                     /translation="RVEAPALGGSPAEDGSFPVVILSHGWTGSRTMHADLAEELASRG
                     IVAIAVDHPYGALAVAFPDGEVKNLYRGALPSRSDGVEAFDRASTFLEETYAADIAAV
                     VGVVRGGRAVPGLEGRLDGGRIALVGHSTGGGAALLAAMIAETAAESAAESAATATES
                     GVVGFLGVVGLDAWVEPIAARLDEGLATPQLHVGSASWAGGRNAELLGRLRAASGEWS
                     ETRIPGSSHADFAMLRYVTSAGSVTGLAGERDGFAFAADVTPLVCDWLEARLSR"
BASE COUNT          162 a          397 c          382 g          189 t
ORIGIN      
        1 ccgcgtcgag gctcccgccc tcggcgggtc gccagccgag gacgggagct tcccggtcgt
       61 catcctgtcg catggctgga ccggctcgcg cacgatgcac gccgacctgg ccgaggaact
      121 ggccagccgg ggcatcgtcg cgatcgccgt cgaccatccc tacggcgcgc tcgccgtcgc
      181 cttcccggac ggcgaggtca agaacctgta ccgcggcgcc ctgccgtcgc ggtcagacgg
      241 cgtggaggcc ttcgaccgcg cttccacctt cctcgaggag acctacgcgg ccgacatagc
      301 cgccgtcgtc ggagtcgtcc gcggcggccg cgccgtgccg ggcctcgagg gccgcctgga
      361 cggcgggcgc atcgccctgg tcggccactc cacgggaggc ggcgcggccc tgctcgccgc
      421 gatgatcgcc gaaacggcgg ccgaatcggc ggccgagtcg gcggcgacgg cgaccgagtc
      481 gggcgtagtc ggcttcctgg gcgtggtcgg cctcgacgcc tgggtcgagc ccatagccgc
      541 gcggctcgac gaaggcctgg ccacgccaca gctccacgtc ggtagcgcct cctgggccgg
      601 tggacgcaac gccgagctgc tcgggcggct gcgcgcggcc agcggcgagt ggtccgagac
      661 gaggataccc ggctccagcc acgccgactt cgcgatgcta cgatacgtga cctcggccgg
      721 ctcggtgacg ggcctggccg gcgagcgaga cggcttcgcc ttcgccgccg acgtcacgcc
      781 gctcgtctgc gactggcttg aggcgaggct gagccgctag cgcgctctgt atgaagccgc
      841 acgccgtaaa cgccggcgtc gcggcgactc gacaattgcg tgcatcaaat ttatcgtttt
      901 ctggtaaaat ttgaatgcat gctacgatat ctgatgatat actgatacat aacagtaatg
      961 tgtcgcctcg cgtagcatcc agcctgttct tccgcctcga cgagtttcgt atatgtcctg
     1021 aaaggagccg cgatgagcgt tccttgtgat aggcagctat tccgcctcgc cctggcgtcc
     1081 gtgatgacgg cgtccatggt cctcgccctc ggcgcccaag acgtccgcgt
//
LOCUS       DAOOLN010000919         1129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_173131_length_1129_cov_2.580074, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000919 DAOOLN010000000
VERSION     DAOOLN010000919.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173131_length_1129_cov_2.580074"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1129)
                     /gene="glgP"
                     /locus_tag="PLW80_10825"
     CDS             complement(<1..>1129)
                     /gene="glgP"
                     /locus_tag="PLW80_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPB67051.1"
                     /translation="PGLPTDEVPIGYVTNGVHPRTWIPYTLLEVLDRYFGPRFAEDPT
                     NLDIWDRIDRISDDELWRTHERRRERLVAFARDRLKKEMRRMGVSEKGLARADDALSP
                     SALTISFARRFATYKRGTLLLTDPDRLLRLLSDKDRPLQIIFAGKAHPHDMPGKEIIK
                     ELVHFSRRDDVAGRIVFLEDYDMTMARYMTSGSDVWLNTPRRPLEASGTSGMKAGMNG
                     VLNCSILDGWWAEGYAPECGWAIGKGEEYVDTELQDEVESKALYDLLEREIIPTFYER
                     GRDGLPRDWIRMMKCSIRDVGKNFSFHRTLMEYTERYYLPALENARRLEAEGFGKAKE
                     TAEYLELARREWQHVAVEDVRSTARPVMERGDAVRVEARVRT"
BASE COUNT          158 a          378 c          389 g          204 t
ORIGIN      
        1 ccgtgcgcac gcgcgcctcg acgcgcaccg cgtcgccgcg ctccatcacc ggcctggccg
       61 tcgacctgac gtcctcgacg gccacgtgct gccactcgcg cctggccagc tccaggtact
      121 ccgcggtctc cttggcctta ccgaagccct cggcctcgag cctgcgcgcg ttctcgagcg
      181 ccggcaggta atagcgctcg gtatattcca tcagcgtgcg gtggaacgag aaattcttgc
      241 cgacgtcgcg gatggagcac ttcatcatcc tgatccagtc gcggggcagg ccgtcgcggc
      301 cgcgctcgta gaaggtcggg atgatctctc gctccagcag gtcgtagagc gccttggact
      361 cgacctcgtc ctgcagctcg gtgtcgacgt actcctcgcc cttgccgatg gcccagccgc
      421 actcgggagc gtaaccctcg gcccaccagc cgtcgagtat cgagcagttg agtacgccgt
      481 tcatgccggc cttcatgccg ctcgtgcccg aggcctcgag ggggcgcctc ggcgtgttga
      541 gccagacgtc cgaaccgctg gtcatgtagc gggccatcgt catgtcgtag tcctcgagga
      601 acacgatgcg gcccgccacg tcgtcgcggc gcgagaagtg caccagctcc ttgatgatct
      661 ccttgcccgg catgtcgtgc gggtgggcct tgcctgcgaa gatgatctgc agcggccggt
      721 ccttgtccga caagagcctg agcaggcggt cggggtcggt caggagcagg gtgcctcgct
      781 tgtaggtggc gaagcggcgg gcgaagctga tcgtgagcgc ggagggggac agcgcgtcgt
      841 cggccctggc caggcccttc tcggagacgc ccatgcggcg catctccttc ttgagccggt
      901 cgcgggcgaa cgcgacgagg cgctcgcggc ggcgctcgtg ggtgcgccat agctcgtcgt
      961 cgctgatccg gtctatgcgg tcccagatgt ccaggttggt cgggtcctcg gcgaagcggg
     1021 gcccgaaata acggtcgagc acctcgagca gggtgtacgg tatccaggtg cgcgggtgga
     1081 cgccgttggt cacgtagcct atcggcacct cgtcggtcgg caggcccgg
//
LOCUS       DAOOLN010000920         1129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_173168_length_1129_cov_2.313780, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000920 DAOOLN010000000
VERSION     DAOOLN010000920.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173168_length_1129_cov_2.313780"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..795)
                     /locus_tag="PLW80_10830"
     CDS             complement(82..795)
                     /locus_tag="PLW80_10830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPB67052.1"
                     /translation="MGLDLDYLARTLLLSLRGLPVTLGLCVATLVVSLPLGFMLAIVG
                     IYRVRVLRKLASVYISFARGTPIVLQILVVYSVLPSLANQVAIRAGWDVDVFALNPLW
                     YAVVVFSFNTTAVLSEIVRSAILTVDRGQYEAALSSGMTSRQAYRRIIVPQALLVALP
                     NLANATINLIKGTSLAFLMAVRDVTAIAKIEAARGYDYVESYLAVFIVYVFVCGATQA
                     LFTLIERRLGIYRAPVVAF"
     gene            complement(810..>1129)
                     /locus_tag="PLW80_10835"
     CDS             complement(810..>1129)
                     /locus_tag="PLW80_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPB67053.1"
                     /translation="EAALSHGLSEFQAFRRIVLPQAAVSALPNVATALVALLKEGSLA
                     YTVGLVDLMGQGSLIIARGFGAWSLETYLAAALIYWALTVAIEKAAGALERRLSVGRR
                     GLA"
BASE COUNT          207 a          352 c          400 g          170 t
ORIGIN      
        1 tcgacgtctc ggagcacctc ggtccgatgg aagctcttgt gcaggtctcg tatgctcagc
       61 atgccggctc tccgcgcctt tttagaacgc tacgacgggc gctcggtaga tgccgaggcg
      121 ccgctcgatg agggtgaaca gggcttgcgt ggccccgcag acgaatacgt atactatgaa
      181 gaccgccagg tacgactcga cgtagtcgta gccgcgcgcc gcctctatct tggcgatggc
      241 ggtcacgtcg cggacagcca tcaggaaggc cagcgaggtg cccttgatca ggttaatcgt
      301 cgcgttggcc aggttcggga gggcgacgag cagggcctgg ggcacgatga tgcgcctata
      361 ggcctggcga ctcgtcatgc cgctcgatag cgccgcctcg tactggccac ggtcgacggt
      421 caggatggcc gaccgtacga tctccgatag caccgccgtg gtgttgaagc tgaagacgac
      481 cacggcgtac cagagcgggt tcagcgcgaa gacgtcgacg tcccagccgg cgcgtatggc
      541 gacctggttg gccaggctcg gtaggacgct gtagaccacg agtatctgga ggacgatggg
      601 cgtgccgcgc gcgaaggaga tgtagaccga ggcgagcttc cgtagcacgc gcacccggta
      661 gataccgacg atggcgagca tgaagccgag cggcagcgag acgacgaggg tcgcgacgca
      721 gaggccgagc gtgaccggca gcccgcggag cgatagtagc agggtgcgcg ccagatagtc
      781 gaggtcgagt cccatacgat gcgcggcccc taggcgaggc ccctccttcc gaccgacagg
      841 cggcgctcca gggcgccggc ggctttctct atggccacgg tcagcgccca gtagatcagg
      901 gccgccgcga ggtaggtctc gagcgaccag gccccgaagc cgcgcgctat gatcagcgag
      961 ccctggccca tcaggtcgac caggccgacg gtgtaggcga gcgaaccctc cttgagcagg
     1021 gcgaccagcg ccgtggcgac gttcggcagg gccgatacgg ccgcctgagg cagcacgatg
     1081 cgacggaagg cctgaaactc gctcaggccg tggctcaggg ccgcctctc
//
LOCUS       DAOOLN010000921         1128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_173384_length_1128_cov_2.370923, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000921 DAOOLN010000000
VERSION     DAOOLN010000921.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173384_length_1128_cov_2.370923"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1128
                     /locus_tag="PLW80_10840"
     CDS             <1..>1128
                     /locus_tag="PLW80_10840"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting membrane transporter"
                     /protein_id="HPB67054.1"
                     /translation="LGSVKPDIPTISVGLGTCGIGNGADVLFDALRDASDGRVAIKKA
                     GCFGFCAEEPLVMLYRPRLPILLFSDMKASKAGKLVTAALDDAACAKLAKTARAKISS
                     WDFRTSTIDYGHGFDALPEWNELAFFQGQEKLVLRDCGLIDPESLAEYAAVGGYSALL
                     KAIEGEPEDVIDEVTRAKLRGRGGAGFPTGVKWKLMRQNEAPIKYVVCNADEGDPGAY
                     MNRNEIESDPHMLLEGMAIGAYAMGAAEGLVYVRAEYPLAVERLKKAIADATAAGFLG
                     ERIMGSGFSFAVRIVTGAGAFVCGEETALIASVEGKAGRPSPRPPFPAQKGYRGMPTN
                     INNVETWCNVPVVVARGAEYFTGIGTEKSPGTKVFSLVGKV"
BASE COUNT          205 a          374 c          383 g          166 t
ORIGIN      
        1 gcctcggctc ggtcaagccg gacatcccga ccatatccgt cgggctcggc acctgcggca
       61 tcggcaacgg ggccgacgtc ctgttcgacg cgctccgcga cgcctcggac ggcagggtcg
      121 ccatcaagaa ggcgggttgc ttcggattct gcgccgagga gccgttggtc atgctctacc
      181 ggccgaggct ccccatactg ctcttctcgg acatgaaggc gtccaaggcc ggcaagctgg
      241 tgaccgccgc cctcgacgac gcggcctgcg ccaagctggc gaagacggcc agggccaaga
      301 tatcgagttg ggacttcagg acctccacga tcgattacgg ccacggcttc gacgcgctgc
      361 ccgaatggaa cgagcttgcc ttcttccagg gccaggagaa actggtcctg cgggactgcg
      421 gcctcatcga tcccgagagt ctggcggagt acgcggccgt cggaggctac tcggccctcc
      481 tcaaggcaat cgagggcgag ccggaagacg tcatcgacga ggtgaccagg gccaagttgc
      541 gcggccgcgg gggcgcaggc ttcccgaccg gcgtgaaatg gaagctgatg cgccagaacg
      601 aggccccgat caagtacgtc gtctgcaacg cggacgaggg cgaccccggc gcgtacatga
      661 accggaacga gatagagagc gacccgcaca tgctgctcga aggcatggcc ataggcgcct
      721 acgcgatggg cgcggccgag ggcctcgtct acgtgcgcgc cgaatacccc ttggccgtcg
      781 agcgcctcaa gaaggccata gccgacgcca cggcggccgg cttcctcggc gagcgtatca
      841 tgggtagcgg cttctccttc gccgtcagga ttgtcaccgg cgccggcgcc ttcgtctgcg
      901 gcgaggagac ggcgctgata gccagcgtcg aaggcaaggc cgggcgcccg tcgccgaggc
      961 cccccttccc ggcccagaag ggctaccgtg gcatgccgac gaacatcaat aacgtcgaaa
     1021 cctggtgcaa cgtgccggtc gtggtcgcgc gcggcgccga gtacttcacg ggcatcggca
     1081 ccgagaagag cccgggtacg aaggtcttca gcctcgtagg caaggtca
//
LOCUS       DAOOLN010000922         1127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_173533_length_1127_cov_4.030784, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000922 DAOOLN010000000
VERSION     DAOOLN010000922.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173533_length_1127_cov_4.030784"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1127)
                     /locus_tag="PLW80_10845"
     CDS             complement(<1..>1127)
                     /locus_tag="PLW80_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67055.1"
                     /translation="EQLLHRLSSEIERNGKDRERLGSELREVDAALARDDNHNLYRRL
                     QDRVRSLEHESEESERKALRCALLRKQCAALLDAARRAEGSDASARSSAAPEDEEALV
                     DHEIGLMEEARSAAERESNETRDALAAAEAALRDRAAELADLERGIRRFPENSRRLVD
                     ELKGRGIEAWILAELADVTNPDWADAVEGWLNTLRFAVVVAPDSFQEALEIYDAQPRS
                     LGGVALPNIAKLRERAGRIRKGSLAELTASDNPWAGIYLNAILGEVMGADIASLKNYD
                     KAITRECMSYSNHTATRVKEEVYREHWLGRAAREKRLRFLNAEVARLRAERVALAERE
                     REATDRSKAFAQGVRSLYEAKQLVNAAAESRRLIDEIAAAK"
BASE COUNT          163 a          375 c          368 g          221 t
ORIGIN      
        1 ttcttcgccg cggcgatctc gtcgatcagg cgacgggact cggcggccgc gttgacgagc
       61 tgcttggctt cgtacagcga gcggacgccc tgggcgaagg ccttggagcg atccgtcgcc
      121 tcgcgttccc gctcggcgag agccacccgc tctgctctga gccgcgcaac ctcggcgttc
      181 aggaacctga ggcgcttctc gcgggcggct ctgcccagcc agtgctcgcg atagacctct
      241 tccttgacgc gcgtggctgt atggttcgag tagctcatgc actcgcgggt tatggccttg
      301 tcgtagttct tcaggctggc gatatcggcg cccatcacct cgccgaggat ggcgttgaga
      361 tagataccgg cccaggggtt atcgctcgcg gtaagctcgg cgagggaacc cttgcggatc
      421 cttccggcac gttcccgcag tttcgcgata ttgggcaggg ctacgccgcc gagcgaacgg
      481 ggttgcgcgt cgtagatctc gagggcctcc tgaaaggaat ccggcgcgac gacgacggcg
      541 aagcgcaagg tattgagcca gccctccacg gcatcggccc aatcaggatt cgttacatcg
      601 gccagctcgg ccaggatcca ggcctcgatg ccgcggccct tcagttcgtc aaccaggcgg
      661 cgggaattct ccgggaaacg gcggatgcct cgttccaggt cggcgagctc ggcggcgcga
      721 tcgcgtaagg cggcctcggc ggcggccagg gcgtcccgcg tttcgttgct ctcccgttcg
      781 gctgcggaac gagcttcttc catcaggccg atctcgtggt cgacgagggc ttcctcgtct
      841 tccggcgcgg cggaagaccg cgccgacgcg tccgagccct ctgctcgccg agcagcgtcc
      901 agtagggcgg cgcactgctt cctgagcaac gcgcaacgta gggccttgcg ctcggactct
      961 tcgctctcat gctcgagcga gcgaaccctg tcctgcagtc tacggtacag gttgtggtta
     1021 tcgtcgcgag ccagggccgc gtccacctcc cgtagctcgg acccgagccg ttccctatcc
     1081 ttgccgttgc gctcgatttc gcttgaaagc ctatgcagga gctgctc
//
LOCUS       DAOOLN010000923         1127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_173535_length_1127_cov_3.955224, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000923 DAOOLN010000000
VERSION     DAOOLN010000923.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173535_length_1127_cov_3.955224"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /gene="ftsY"
                     /locus_tag="PLW80_10850"
     CDS             <1..824
                     /gene="ftsY"
                     /locus_tag="PLW80_10850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPB67056.1"
                     /translation="RVGADGGGAAVVEGDLGAAFADESVSRLKEACATAGVRNGDGAR
                     RLLKDVLRPYAVPVDIRPIPGGRNVVMMLGVNGVGKTTSSAKLAKLWLGLGLATKAIL
                     AAGDTFRAAAIEQLKAHGDRLGVRVVAQERGSDSGAVLYDALEAAAADGSDLVIADTA
                     GRMHTKANLIKELEKMDKIVAAKADPSRYKRLLVVDATTGQNALRQAEAFSAAVKVDG
                     LVLTKYDSSARGGVALAIARQLGIPTAFVCDGERYEDIRAFDPETYLDQFLGLTP"
     gene            821..>1127
                     /locus_tag="PLW80_10855"
     CDS             821..>1127
                     /locus_tag="PLW80_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67057.1"
                     /translation="MRRHAFLLMAGLSAALALPLAAQVATGWYVSDPSGLAIEPTAGP
                     GGAEYALLVTGSGGSFRSELYRDGEAVRAWLRTYGRRGAIVREAMEEGGSIREERLYD
                     "
BASE COUNT          181 a          383 c          402 g          161 t
ORIGIN      
        1 agcgcgtcgg agcggacggt ggcggtgcgg ccgtcgtcga gggtgacctc ggcgcagcct
       61 tcgccgatga gtccgtctcg cggctcaagg aggcgtgcgc gacagcgggc gtcaggaacg
      121 gcgatggcgc gaggcgcctc ctcaaggacg tgctccggcc ctacgccgtg cccgtagaca
      181 tacggccgat acccggcggg cgcaacgtcg tgatgatgct cggcgtgaac ggcgtcggca
      241 agacgacgag ctcggcgaag ctggccaagc tgtggctcgg cctggggctg gccacgaagg
      301 ccatactggc ggccggagac accttcaggg ccgcggccat cgagcaactc aaggctcatg
      361 gcgaccggct cggcgtcagg gtcgtcgccc aggagcgcgg ctccgactcg ggcgcggtgc
      421 tctacgatgc cctcgaggcc gcggcagccg acggctccga cctggtgata gccgataccg
      481 ccggccggat gcataccaag gccaacctga tcaaggaact cgagaagatg gataagatcg
      541 tcgcagccaa ggccgatccg tccaggtaca agcgcctcct cgtcgtcgac gcgacgaccg
      601 gccagaacgc gctcaggcag gccgaggcct tctcggcggc cgtcaaggtg gatgggctcg
      661 tcctaaccaa atacgactcg agcgccaggg gcggcgtggc cctggccatc gccagacagc
      721 tcggcatccc gacggccttc gtctgcgacg gcgagcgcta cgaggacata cgggccttcg
      781 atcccgagac ctacctcgac caattcctgg gactgacgcc ttgagacgac atgccttcct
      841 cctcatggcc gggttatcgg ccgccttggc gctgccgctc gcggcccagg tcgctaccgg
      901 atggtacgtc tccgacccgt cgggcctggc catcgagccg accgccgggc caggcggtgc
      961 ggagtacgcg ctcctcgtca cgggctcggg cggctcgttc cgctcggagc tgtatcgcga
     1021 cggcgaggcg gtccgggcct ggctccggac ctacggccgg cgcggtgcca tcgtccgcga
     1081 ggccatggag gagggcggct cgatacgcga ggaacggctg tacgacg
//
LOCUS       DAOOLN010000924         1127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_173545_length_1127_cov_3.525187, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000924 DAOOLN010000000
VERSION     DAOOLN010000924.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173545_length_1127_cov_3.525187"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="PLW80_10860"
     CDS             complement(<1..875)
                     /locus_tag="PLW80_10860"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein"
                     /protein_id="HPB67058.1"
                     /translation="MRTARASDLSMVGRLSDFPLLDAVYVGTVRSPVSRGAIRSISVP
                     HLPRGYRSVTADDIPGSARIDSIGASVPVLASGRVAYRGEPVALIVGPDRLRVAEAVS
                     MTAVRVDEEPASFGYEACESRRLVAAYSTDSGSVDAALAKAARVVDGEYRMGPQDHYY
                     PEPQGAAAGFDYDKLIVFSSTQWPFQARDAVAQALGVRKDEVVVRTTSLGSHLDGKLW
                     YPSLLACHAAIAARLSGKNALLLLSRTDDFMYTTKRAPTLVSYRAGLDEAGKLVAMDA
                     SVIINVGAYAPFGAEL"
     gene            complement(872..>1127)
                     /locus_tag="PLW80_10865"
     CDS             complement(872..>1127)
                     /locus_tag="PLW80_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPB67059.1"
                     /translation="RGGLARDEFIESLAERGVGTSVHFIPLHTMPYYAGRYGLEPGSM
                     PNAMAMFERTVSLPIWQGMGLDAARRVADAVLAVAEALHA"
BASE COUNT          173 a          390 c          388 g          176 t
ORIGIN      
        1 agctccgcgc cgaacggcgc gtaggcgccg acgttgatta tcacgctcgc gtccatcgct
       61 acgagtttgc cagcctcgtc gagtccggcc cggtacgaga cgagggtcgg cgcccgtttc
      121 gtcgtgtaca tgaagtcgtc ggtcctcgat aggagcagga gcgcgttctt gccgctcagc
      181 ctggcagcga tcgccgcgtg gcaggccagg agcgacgggt accatagctt gccgtccagg
      241 tgggagccga gcgacgtggt ccggaccacc acctcatcct tcctgactcc gagcgcctgg
      301 gcgacggcgt ccctggcctg gaagggccat tgagtcgacg agaaaacgat cagcttgtcg
      361 tagtcaaagc cggccgcggc gccctggggc tccgggtagt aatggtcctg cggacccatc
      421 ctgtattctc cgtcgaccac ccgggcggcc ttggccagcg ccgcgtcgac ggagccgcta
      481 tcggtcgagt aggcggcgac gagtctcctc gactcgcagg cctcgtatcc gaacgacgcc
      541 ggctcctcgt cgacgcgtac cgccgtcatc gatacggcct cggctaccct gagccggtcc
      601 ggcccgacga tcagcgccac cggctcgccg cggtacgcga cgcgccccga ggccaggacg
      661 ggcaccgagg cgcctatact gtcgatacgg gccgagcctg ggatatcgtc cgccgtgacg
      721 ctccggtagc cgcgtggcag gtgcggcacg ctgatggatc gtatggctcc cctcgatacg
      781 ggcgagcgca ccgtcccgac gtagaccgcg tcgaggagag ggaaatccga caggcgccct
      841 accatcgaga ggtcgctcgc ccgggccgtc ctcatgcgtg cagcgcctcc gcgacggcta
      901 ggacggcgtc ggcgacgcgc cgcgccgcgt cgagtcccat gccctgccag atcggcaggc
      961 tgacggtgcg ctcgaacatg gccatggcgt tgggcatgga tcccggctcg aggccgtagc
     1021 gccccgcgta gtagggcatg gtgtggagcg ggatgaaatg gaccgacgtg ccgacgcccc
     1081 gctcggcgag cgactcgatg aactcgtccc tggcgaggcc tccgcgc
//
LOCUS       DAOOLN010000925         1126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_173870_length_1126_cov_2.714286, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000925 DAOOLN010000000
VERSION     DAOOLN010000925.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173870_length_1126_cov_2.714286"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1126)
                     /locus_tag="PLW80_10870"
     CDS             complement(<1..>1126)
                     /locus_tag="PLW80_10870"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPB67060.1"
                     /translation="ERSPAWAERICGVPAEVIEGFARRYAAARPAMLVPGYSIQRVAR
                     GEETFRLSVALQVATGNLGVPGGSSGSLNNRLPGVRTGAMSDLADRLVGDARTLTVPV
                     LRWPDAILSGRSGGFPGDVKAAYVAGFNALGQGADNRKSARALESLEFSVCHEMFMTP
                     TARRCAVVLPILSPLEKEDACAPWHGNYLLYKPAALPPRGLARSDYDIFAELSERLGA
                     GEAFSEGLSATGWPDRLLAESEIRDLEAFKATGVYFGADRERVGLARFASDPEGHPLP
                     TPSGRIELRSDAYASDTGEAAIPTWKDSEPDPSLPFLLVTPKTIRRTHSQLAYGPGPD
                     LGELFVSFEDAARLSLASGDRVEVGNASGRLLATAAVTDDI"
BASE COUNT          165 a          380 c          413 g          168 t
ORIGIN      
        1 gatatcgtcc gttaccgccg cggtagccag cagcctgccc gaagcgttac cgacctcgac
       61 ccggtcgccg gaggccagcg acaggcgggc ggcgtcctcg aatgatacga acagctcccc
      121 aaggtccggt cccggcccgt acgcgagctg ggagtgggtg cgcctgatcg tcttcggcgt
      181 cacgaggagg aagggcaggg acgggtcggg ctcgctatcc ttccaggtcg ggatggccgc
      241 ttcgcccgta tcgctggcgt aggcgtcgct ccgtagctct atccgcccgg acggcgtcgg
      301 caggggatgg ccttcagggt cggaggcgaa gcgggccagc cccacgcgct cgcggtcggc
      361 gccgaagtag acgcctgtcg ccttgaacgc ctcgaggtcg cggatttcgg attcggctag
      421 gagccggtcg ggccagcccg tagcgctcag gccctccgag aaggcttcgc ctgcgccgag
      481 acgctccgac agctccgcga agatgtcgta gtcgctcctg gccaggcccc gcggcggcag
      541 cgcagcgggt ttgtacagca ggtagttgcc gtgccagggc gcgcaggcgt cttccttctc
      601 gagcggcgag aggatcggta gtaccacggc gcatcgcctg gccgtcggag tcatgaacat
      661 ctcgtgacag accgagaact cgagcgactc gagcgctcgg gccgattttc ggttgtcggc
      721 gccctggccc agcgcgttga agccggccac gtaggcggcc ttgacgtccc ccgggaatcc
      781 gcccgagcgg ccggacagga tggcgtcggg ccagcgtagc accggcacgg taagggttcg
      841 ggcgtcgccg actaggcggt cggccaggtc cgacatcgct cccgtgcgca cgccgggcag
      901 gcgattgttg agcgagcccg acgagcctcc cgggacgccg aggttgccgg tcgctacctg
      961 cagggcgacc gatagcctga aggtctcctc gccgcgggcg acgcgctgga tcgagtagcc
     1021 gggtacgagc atggccggcc tcgccgcggc gtagcggcgg gcgaagcctt cgatgacctc
     1081 ggccggaacg ccgcagatcc gctcggccca ggccggcgag cgctcg
//
LOCUS       DAOOLN010000926         1126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_173902_length_1126_cov_2.338002, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000926 DAOOLN010000000
VERSION     DAOOLN010000926.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173902_length_1126_cov_2.338002"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..477)
                     /locus_tag="PLW80_10875"
     CDS             complement(4..477)
                     /locus_tag="PLW80_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67061.1"
                     /translation="MQTGGAALHRRGRDYRMNARVVYYSATGNTRKVADAIAGAVGLE
                     ASTIDAYADAPVDLLFLGAAVYATHDHGLHAGMKAFIAALDPAKVGRVALFSTGFVHS
                     EAIAMMRALLERRGIPVAEKSFFCLGRFALFNLGRPNAKDLAAAADFARGLIARP"
     gene            complement(551..994)
                     /locus_tag="PLW80_10880"
     CDS             complement(551..994)
                     /locus_tag="PLW80_10880"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB67062.1"
                     /translation="MDDGLVYRKLNVGDIDSVVRMETDFRPGFIVQENAERFLRDPMN
                     WLIACLSGDRIVGFAYGYELYRLNGIGNMVYIHEVGVLPEYQRQGIGTELLQTIRRLC
                     KSTGICRFFLFTERSNTGACSLYRKMKGEEAHTDDVAYFFSDLDE"
BASE COUNT          226 a          337 c          336 g          227 t
ORIGIN      
        1 ctactacgga cgggcgatca ggccccgggc gaagtcggcg gcggccgcga ggtccttggc
       61 gttcgggcgg cccaggttga agagcgcgaa gcgcccgagg cagaagaagc tcttctcggc
      121 cactggtatg ccgcgccgct cgagcagggc gcgcatcatc gctatggcct cgctatggac
      181 gaagccggtc gagaacagcg cgaccctgcc gaccttggcc gggtccagag cagcgatgaa
      241 cgccttcatg ccggcgtgca ggccgtggtc gtgcgtggcg tagacggccg cgccgagaaa
      301 gagcaggtct accggcgcgt ccgcgtacgc gtcgatcgtc gacgcctcga gcccgacggc
      361 tcccgcgatg gcgtctgcca ccttcctcgt gtttccggtg gccgagtaat agacgacccg
      421 agcgttcatg cggtagtccc ttccacggcg atggagcgcc gcgcccccag tctgcacgcg
      481 acggcctttt cttgcaagcg gaatagcacg acgcgccgcg gcgggcggtc gtcggactcg
      541 gtccgtgaat ctactcgtcc aggtcgctga agaaataggc gacgtcgtca gtatgggctt
      601 cttcgccttt catctttcta tataacgagc aggccccggt attcgatcgt tcagtaaata
      661 ggaagaacct gcatattccc gtcgatttgc agagccttcg tatcgtttgt aataattcgg
      721 tgccgattcc ttgtctctga tattcgggta agacgccgac ctcatggata tagaccatat
      781 tcccgatacc gttcaggcga taaagctcat acccatacgc gaaaccgact atcctatcgc
      841 cggacaggca ggcgatcagc cagttcatgg gatcccgcag gaatcgttcg gcgttttctt
      901 gtacgatgaa cccgggcctg aaatcggttt ccatccggac taccgaatcg atatcgccga
      961 cgttcagttt cctgtaaacg agtccgtcat ccataccggc ttccctcgac ctaaggatgc
     1021 gatcatcata gcgagtatga aggaatagtc atacaggacg cggatgcggg acgatggacc
     1081 cggaaggtcg cggctagcct ctacaaggtt cgaggaacag actata
//
LOCUS       DAOOLN010000927         1125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_174086_length_1125_cov_3.125234, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000927 DAOOLN010000000
VERSION     DAOOLN010000927.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174086_length_1125_cov_3.125234"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            323..871
                     /locus_tag="PLW80_10885"
     CDS             323..871
                     /locus_tag="PLW80_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPB67063.1"
                     /translation="MKTFVCSVCGYVYVGEEAPAQCPQCKAPKVKFVEKAAVSTGAFA
                     DEHRIGVAKGLDAEVVQGLRDNFMGECTEVGMYLAMSRQADREGYPEIAEAYRRYAFE
                     EADHAARFAELLGEVVHADTKLNVSLRAEAEYGACEGKLMLAKRAKELGYDAIHDTVH
                     EMCKDEARHGAGFRGLLARYFK"
     gene            921..>1125
                     /locus_tag="PLW80_10890"
     CDS             921..>1125
                     /locus_tag="PLW80_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPB67064.1"
                     /translation="MDTKGFLRSHGIQPSQQRLRIYEALAAVKSHPDAETIYRKLIGE
                     LPTLSRTTVFTTLDPSSARAWPSA"
BASE COUNT          238 a          339 c          342 g          206 t
ORIGIN      
        1 ggccaagtcg gccggcacct acaccgggct gctcgtgccc ggcaccgagg tgacgaaaat
       61 tcgcccgacc aagagccggg gggccccatc gaagctgctg tagcttgaag ttttttgatg
      121 gccgcatacg caacctcacg tcaagacttc aaaataagtt ctcgcgtaat cagcagtcac
      181 ttggtttctt tgggaataga gtgctatggc cgccatgcgc cggctagctg gtcgacgctc
      241 agtcaccatg gagcgagggg cttgatattc tgtaataatt actatactgt aataaataca
      301 aataatttcc atgggagata gtatgaagac attcgtctgt tcggtctgcg gctacgttta
      361 cgtcggcgaa gaggcccccg cccagtgccc tcagtgcaag gcgcccaagg tgaagttcgt
      421 cgagaaggcc gccgtatcca ccggcgcgtt cgcagacgag caccgcatcg gcgtggccaa
      481 ggggcttgat gccgaggtcg ttcagggcct tcgtgataac ttcatgggcg agtgcaccga
      541 ggtcggcatg tacctggcca tgagccgcca ggccgatcgc gaaggctatc ccgagatagc
      601 cgaggcctat cgccgctacg ccttcgagga agccgaccac gcggctcgct tcgccgagct
      661 tttgggcgag gtcgtgcacg ccgacaccaa gctgaacgta tcgctacgcg ccgaggccga
      721 gtacggcgcc tgcgagggca agctcatgct ggccaagcgc gccaaagagc tgggttatga
      781 cgctatacac gatacggtac acgagatgtg caaggacgag gctcgccacg gcgccggctt
      841 ccgcgggctg ctcgcgagat atttcaagta ggtacgcggc gggcggctgc acgccgcccg
      901 cactatcata aggagctagg atggatacaa aaggcttcct gcgctcgcac ggcatacagc
      961 catcccaaca gcgcctgcgt atctacgagg cgctcgccgc ggtgaagagc catcccgacg
     1021 ccgagaccat ctataggaag ctgatagggg agctaccgac gctatcgagg accacggtgt
     1081 tcaccacgct cgacccttcg tccgcgcgag cctggcccag cgcct
//
LOCUS       DAOOLN010000928         1125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_174095_length_1125_cov_3.040187, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000928 DAOOLN010000000
VERSION     DAOOLN010000928.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174095_length_1125_cov_3.040187"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..639
                     /locus_tag="PLW80_10895"
     CDS             100..639
                     /locus_tag="PLW80_10895"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HPB67065.1"
                     /translation="MKVARQAMNVALKPRYFWLGALALALGASAIAGALSPPRQAAVL
                     WFPDARGSDPADARSELRYVAVKKDAAEQAAELVEELLLGPMDAMARPIAVPQVRVES
                     VIVSGKTVYVDLSADLCFGKPAADGSYAAPPVEPRVALDYIDRALRWNFRFHDVRLTV
                     QGLEPDWATVKSPEEAQNS"
     gene            723..>1125
                     /locus_tag="PLW80_10900"
     CDS             723..>1125
                     /locus_tag="PLW80_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HPB67066.1"
                     /translation="MRRSVALIVVALLVASASVVFADEAVLIDFSLLGADIIEDPVQA
                     GKLTQNRATMMDFSTVAGASYTEEQKKQMAISLAIENWDVILASSSRTGMRQALTYTR
                     EAKIAQDAKLYAGATALGIRVNFPLEPFNSWA"
BASE COUNT          227 a          349 c          351 g          198 t
ORIGIN      
        1 gctgggcgac ggcatctata atggcatcgt cgagttcgtc gactatttcg aacacaggaa
       61 aggctcgagc gcccagtgaa accgactctc gaacggatcg tgaaggttgc ccgccaagcg
      121 atgaatgtgg cgcttaaacc gaggtatttc tggctcgggg cgctggcttt ggctctcggc
      181 gcaagcgcca tagccggagc gctgtcgccg cccaggcagg ccgcggtgct atggtttccc
      241 gacgcccgcg gctcggaccc cgccgacgcc cgctcggagc tgcgctacgt ggccgtcaag
      301 aaggacgcgg ccgagcaggc cgccgagctc gtggaggagc tgctgctcgg gcctatggac
      361 gcgatggcca ggccgatagc ggtaccgcag gtcagggtcg agtcggtcat cgtctccggc
      421 aagaccgtct acgtcgacct gtcggccgac ctatgcttcg gcaagccggc tgccgacgga
      481 agctacgccg ctcctccggt cgagccccgc gtggcgctcg actatataga cagggcgctg
      541 cgctggaact tcaggttcca cgacgtcagg ctgaccgtcc agggcctgga acccgactgg
      601 gcgaccgtaa aaagccccga agaggctcaa aattcataaa ataattgaca aagggtaggg
      661 cttggctata atcaagtcaa attgggtaaa actgtacgcg cactatggaa aggagcattt
      721 gaatgagaag gtcagtagca ctgatcgtcg tcgctctgtt ggtcgcgagc gcatcggtcg
      781 ttttcgcgga tgaggccgtc ctgatagatt tttccctcct cggagcggat ataatcgagg
      841 accccgtcca ggccggtaag ctgacgcaga accgcgccac catgatggat ttctcgacgg
      901 tcgcgggcgc gagctacacc gaggagcaga agaagcagat ggccatctcc ctggccatag
      961 agaactggga cgtgatactc gcctcgtcct ccaggaccgg catgcgccag gcgctcacgt
     1021 atacccgcga ggccaagatc gcccaggacg ccaagctgta cgccggcgcc accgccctcg
     1081 gcatccgcgt caacttcccg ctcgagccct tcaactcctg ggcca
//
LOCUS       DAOOLN010000929         1120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_175397_length_1120_cov_2.151174, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000929 DAOOLN010000000
VERSION     DAOOLN010000929.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175397_length_1120_cov_2.151174"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..>1120
                     /locus_tag="PLW80_10905"
     CDS             241..>1120
                     /locus_tag="PLW80_10905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPB67067.1"
                     /translation="MRVSLIAPIYNEYENLPDLVAKVESVITTQPRAWEFVCVNDGSR
                     DGSDVLLEKLAATRPWLRPVFLRRNYGQSTAMQAGFDAARGEVLVTIDGDMQNDPADI
                     PRLLDMLDAKPEIDIISGWRRHRQDKTVSRKIPSMIANRLISKVTGVSLHDYGCSLKL
                     YRRSAVRHVKIYGELHRFIPALAHEYGAKVMEAEVNHFPRTKGVSKYGIDRTIRVLLD
                     LLWVKFLLRFLHRPMHAFGGLGAMLFFPGLLILAYLAGIKLFLGAAIGGRPLLLLGVM
                     FTLMGANFIGLGILGEM"
BASE COUNT          177 a          389 c          342 g          212 t
ORIGIN      
        1 cggtgtcggc gtcgatccgg tctggcgtat cggcctgggc gtgctgctct tcgtcgtttc
       61 gggcgtactc ggcgtgtacc tgtaccggcg catgcgtcac ggcaagagct atgacgaggc
      121 gctggaccgg gatatcgacg cctgaccggc cgctaaggtt ttgccgcgtg gcgcctgtta
      181 aactagcgcc tcttaaattt ccaccccgcc aacatgcctc tgcccgacgc cagtgccgac
      241 gtgcgcgtat ccctgatcgc gccgatctac aacgagtacg aaaacctgcc agacctggtc
      301 gccaaggtgg agtcggtgat aacgacccag ccgcgcgcct gggagttcgt ctgcgtcaac
      361 gacggttcac gcgacggttc ggacgtccta ttggagaaac tcgcggctac gcgcccctgg
      421 ctgcgtcctg tctttctgcg ccgcaactac ggccagtcga ccgccatgca ggccggcttc
      481 gacgcggcgc gcggcgaggt tctggtgacc atcgacggcg acatgcagaa cgacccggcc
      541 gatatcccgc gccttctcga catgctcgac gcaaagcccg agatcgacat catctccggc
      601 tggcgccggc accgccagga caagacggtg tcgcgcaaga tcccgtcgat gatcgccaac
      661 cgcctcattt ccaaggtcac cggggtcagc ctgcacgact acggttgctc cctgaagcta
      721 taccggcgca gcgccgtgcg ccacgtgaag atctacggcg agctgcaccg cttcatcccc
      781 gccctggcgc acgagtacgg cgccaaggtg atggaggccg aggtcaacca cttcccgcgc
      841 accaagggcg tctccaagta cggcatcgac cggaccatcc gcgtcctgct cgacctgctc
      901 tgggtgaagt tcctgctgcg cttcctgcat cgcccgatgc acgccttcgg cggcctcggc
      961 gccatgctct tcttccccgg cctgctgatc ctggcctacc tggcgggcat caagctgttc
     1021 ctgggcgcgg ccatcggcgg ccggccgttg ctgcttctgg gcgtgatgtt cacgctgatg
     1081 ggcgcgaact tcatcggcct gggcatcctt ggcgagatgc
//
LOCUS       DAOOLN010000930         1119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_175561_length_1119_cov_2.799812, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000930 DAOOLN010000000
VERSION     DAOOLN010000930.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175561_length_1119_cov_2.799812"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..1039
                     /locus_tag="PLW80_10910"
     CDS             188..1039
                     /locus_tag="PLW80_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPB67068.1"
                     /translation="MGDTSESNYAVAMRRFTTGYNKGVVLSSVTRHSLEILDESMERI
                     DAGLSAVVAAFEEIRATSGSTAANAERIDSMMADVLAGNAGLAAGITERMGDVERGAA
                     VAKDLGARFDGLKAKTEGVAGITGAIQDVSDRTNILAINASIEAARAGSVGKGFRIIA
                     NEVRTLAGQTSDFAKQIEASLGELTGAVGDIAARMDEFVQLFSRFRESFAEVRANSDE
                     NAGSLQRAGDFLRQISGAIREETTALGDGLHSLEAISSSVKETHAVFGGLRSSHEFLD
                     TLLDKEP"
BASE COUNT          182 a          402 c          383 g          152 t
ORIGIN      
        1 aaacgcctca tagcctctaa ttccgtggcc aaacgctggg tcgagaacgc gcggtcctcg
       61 gccccgagct gatcgcgccc cagcacggcg ccgtctaccg cggcgcctcg gtccgggcct
      121 tcctcgactg gctgtccggc ctccgctgcg gcgtcgacga gctcgacgcg ctgtacagga
      181 tgtagccgtg ggcgacacga gcgaatcgaa ctacgccgta gcgatgcggc gcttcaccac
      241 cgggtataac aagggcgtcg tgctgtcgtc cgttacccgg cactccctcg agatactcga
      301 cgagtcgatg gagcgcatcg acgcgggcct gtcggcggtc gtcgccgcct tcgaggagat
      361 cagggcgacg agcggctcga ccgccgccaa cgccgagcgc atcgactcga tgatggccga
      421 cgtgctcgcc ggcaacgcgg gcctcgccgc cggcatcacc gagcgcatgg gcgacgtcga
      481 gcgcggcgcc gccgtggcca aggacctcgg cgccaggttc gacggcctca aggccaagac
      541 cgagggcgtc gccgggatca ccggcgccat ccaggacgtc tccgaccgca ccaacatcct
      601 cgcgatcaac gcctcgatcg aggccgctcg cgccggatcc gtaggcaagg gcttccgcat
      661 catcgcgaac gaggtccgga ccctcgcggg ccagaccagc gacttcgcca agcagatcga
      721 ggcgagcctc ggcgagctga ccggggccgt cggcgacata gcggcgcgca tggacgagtt
      781 cgtccagctg ttcagccgct tccgcgagtc gttcgccgag gtcagggcca actccgacga
      841 gaacgccggc agcctccagc gcgccggaga cttcctccgg cagatatccg gcgccatccg
      901 cgaggagacg acggcgctcg gcgacgggct ccactcgctc gaggccatca gctcgagcgt
      961 caaggagacg catgccgtgt tcggcggcct gcgctccagc cacgagttcc tggatacgct
     1021 cctggacaag gagccatgac ggccggcgcc ggggagggcc ggctgctcgc ggtcgtcacc
     1081 gacgaccgct tcggcggcgt ggccgaggag gcggcggtg
//
LOCUS       DAOOLN010000931         1119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_175594_length_1119_cov_2.499060, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000931 DAOOLN010000000
VERSION     DAOOLN010000931.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175594_length_1119_cov_2.499060"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..449
                     /locus_tag="PLW80_10915"
     CDS             108..449
                     /locus_tag="PLW80_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB67069.1"
                     /translation="MRALIIDDDALNRHILRRYLAKLGYVCDEAASGLEGLDACRIAN
                     YDVALVDLILPDLDGSDTATAIREAYASRATPVRLIAVTGARCDERVKRMFDAVVEKP
                     YTLEELSAVIV"
     gene            532..604
                     /locus_tag="PLW80_10920"
     tRNA            532..604
                     /locus_tag="PLW80_10920"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:565..567,aa:Met,seq:cat)
     gene            complement(993..>1119)
                     /locus_tag="PLW80_10925"
     CDS             complement(993..>1119)
                     /locus_tag="PLW80_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPB67070.1"
                     /translation="IMGLWAAGEVTGGIHAGNRLGGNAIADIFTFGRIAGVNAAK"
BASE COUNT          234 a          305 c          352 g          228 t
ORIGIN      
        1 tattatgctg acgacgaccg ggacgcctta cgctgcttac tcgataggat agggcgtatc
       61 ctaggggcgg gcatcgattc gatcgctaac ctgccaggcg gccgtgaatg agggcgctga
      121 tcatcgacga tgatgccttg aaccggcata tactacgcag gtatctagcc aagctagggt
      181 atgtctgcga tgaggcggcc agcgggctcg agggcttgga cgcctgtcgt atcgccaact
      241 acgacgtagc tctcgtcgat ctcattctgc cggaccttga cggctccgat accgctacgg
      301 cgatccgcga ggcctatgcg tcgcgggcta cgcctgtacg gctgatcgcc gtcacgggcg
      361 cgcgctgcga cgaacgggtc aagcgcatgt tcgacgccgt cgtcgagaaa ccgtataccc
      421 tcgaggagct gtcggcggtt attgtatagg catccaagcc acaatcaaga gttcaaagct
      481 tgtagtctat tgacattctt tatcgttttc tgtatcttaa tcacagatcg acgcagggta
      541 gagcagttgg cagctcgtcg ggctcataac ccggaggtcg taggttcgag tcctacccct
      601 gctaaaaaca aaggtcacgc gacagcgtgg ccttttgttt tatataaagc aggggtagga
      661 ctcgaaccat agtgagcgcc gagcggtagc gaggaagagc gcgcatggac gcgcgcggaa
      721 gcgaacggag gcgctcgcag gcctgagcca ggatggcgaa ggccggagag agagtcctac
      781 ccctgctaaa aacaaaggtc acgcgacagt gtggcctttg tcttatggag taggagtagg
      841 actcgaacca ttcgccgcga gccggcgagc ggcagagaag cgccagacgg atcctggtaa
      901 cgctcggaga gaaagaaggg gctgcccgat tttccgggca gtcccttcgt ataatctcgg
      961 tagctgttta ctgtacgatc tgcctgcttg gtctacttgg ccgcgttgac gccggcgata
     1021 cgcccgaagg tgaagatgtc ggcgatggcg ttgccgccga gtcggttgcc ggcatggatg
     1081 cctcccgtga cctctcccgc ggcccagaga cccatgatg
//
LOCUS       DAOOLN010000932         1118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_175954_length_1118_cov_1.818438, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000932 DAOOLN010000000
VERSION     DAOOLN010000932.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175954_length_1118_cov_1.818438"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..466)
                     /locus_tag="PLW80_10930"
     CDS             complement(29..466)
                     /locus_tag="PLW80_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67071.1"
                     /translation="MPRGAGFFSGKMAVILPSAFINSLAVGCMSLGMLFAVKDLFGAG
                     PGLVASLGALWSTAYLIGCIALRVPAGRLRPRTAMTVMLLGSAAAVSAFLAYPGLWQA
                     FASYACFGLLTAFFWPPVMGWLSKGYEGAELNRATSLFSFSWT"
     gene            684..>1118
                     /locus_tag="PLW80_10935"
     CDS             684..>1118
                     /locus_tag="PLW80_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase (NAD(+)) LigA"
                     /protein_id="HPB67072.1"
                     /translation="MESDETRTARIGELERLVVHHQDLYYNGRPELSDAEFDALWDEL
                     STLAPDSPVLAKVGADAADGFPKARHVLPMGSQAKAADPEAFLAWCSKASLPEYVVEH
                     KLDGASLELQYESGRLVRAVTRGDGRVGDDVTPNARRMAGVPA"
BASE COUNT          205 a          371 c          389 g          153 t
ORIGIN      
        1 tcgagcagga gctgggcgag gtctggtact acgtccacga gaagctgaac aggctggtcg
       61 cccggttgag ctcggcgccc tcgtagccct tcgatagcca gcccatcacc ggaggccaga
      121 agaacgcggt caggaggccg aagcacgcgt acgaggcgaa ggcctgccac aggcccgggt
      181 aggccaggaa cgccgacacc gcggccgcgc tccccaggag catgacggtc atggccgtcc
      241 tcggcctcag cctaccggcc gggacgcgca gggcgatgca gccgatcagg taggccgtcg
      301 accagagcgc gccgagcgac gccacgaggc ccgggccggc gccgaacagg tccttgacgg
      361 cgaacagcat gccgagcgac atgcagccga cggccaggct attgatgaac gccgacggaa
      421 gaataacggc catcttaccg gaaaagaaac cagcgcctct cggcatacgt cccgccttcg
      481 ggtcctcgaa cgagcccaag cgaacggtag ttcggccggg cccctcgggt caagcgggga
      541 tgccttcggc gtagtgcctt cggcgtagtg ccttcggcgt agtgccttcg gcgtagtgcc
      601 ttcggcgttg accgggggcg gcggcgcgtc tacaatgccg tttgcgccga gggcgcggca
      661 gacgacacgc gaggcgggga gcgatggaat ccgatgaaac acgaacggcg aggatagggg
      721 agctcgagcg gctcgtcgtc catcaccagg acctctatta taacggtaga cccgagctat
      781 cggacgccga gttcgacgcg ctctgggacg aactgtcgac cctggcgcct gacagcccgg
      841 tgctggccaa ggtaggcgcc gacgcggccg acggttttcc caaggccagg cacgtgttgc
      901 ccatgggtag ccaggccaag gctgccgacc ccgaggcgtt cctggcctgg tgctctaagg
      961 cctccctgcc ggagtacgtc gtcgagcaca agctcgacgg cgccagcctc gagctacagt
     1021 acgagtccgg ccgcctggtg cgcgcggtca cacgcggcga cggccgtgtc ggtgacgacg
     1081 ttaccccgaa cgcccgacgc atggccggcg tacccgcg
//
LOCUS       DAOOLN010000933         1117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_176026_length_1117_cov_3.781544, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000933 DAOOLN010000000
VERSION     DAOOLN010000933.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176026_length_1117_cov_3.781544"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="PLW80_10940"
     CDS             complement(<1..1103)
                     /locus_tag="PLW80_10940"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HPB67073.1"
                     /translation="MVGAARTAVGSFNGALAGVSPVELGRTALAAALERAGLKPADID
                     ELMLGCVLQGGLGQNVARQVAIKAGLPVESTASTVNMVCGSGLRAVANAAQAIKAGDA
                     SIVAAGGTENMSLAPYLLRQARTGYRMGNGELVDSMIHDGLWEIFNGYHMGITAENLA
                     AKFGISREDQDKLAATSQNRAEAAMKGGRFADEIAPVAIPQRKGDPVIVDVDEFPRAG
                     VTAESLGKLRPAFKPDGTVTAGNASGINDGAAALIVADGESVKAKGLKPLARIVSYGW
                     HGVDPAIMGYGPVEAVRLALKRAGWKVGDLDLIEANEAFAAQALSVARELKFDMDKVN
                     VNGGAIAIGHPIGASGARILVTLLYEMRRRNAKR"
BASE COUNT          142 a          411 c          375 g          189 t
ORIGIN      
        1 cgcttggcgt tccgcctccg catctcgtac agcagggtaa cgaggatgcg cgcgcccgag
       61 gcgccgatcg ggtggccgat cgctatcgcg ccgccgttca cgttcacctt gtccatgtcg
      121 aacttgagct cgcgggcgac cgacagggcc tgggccgcga aggcctcgtt ggcctcgatc
      181 aggtcgaggt cgccgacctt ccatccggcc cgcttgagcg ccaggcggac cgcctcgacc
      241 gggccgtagc ccatgatagc cgggtcgacg ccgtgccagc cgtacgagac gatcctcgcg
      301 agcggcttga ggcccttcgc cttgaccgac tcgccgtcgg cgacgatcag cgcggccgcg
      361 ccgtcgttga ttcccgacgc gttgccggcg gtcaccgtgc cgtcgggctt gaacgccggc
      421 cggagcttac cgagcgactc ggccgtcacg ccggctcgcg ggaactcgtc gacgtcgacg
      481 atcacggggt cgcccttgcg ctgcgggatg gcgaccggcg ctatctcgtc ggcgaagcgg
      541 ccgcccttca tcgcggcctc ggcgcggttc tggctcgtcg cggccagctt gtcctggtcc
      601 tcgcggctga taccgaactt ggccgcgagg ttctccgcgg tgatgcccat gtggtagccg
      661 ttgaagatct cccacagacc gtcgtggatc atcgagtcga cgagctcccc gttgcccatg
      721 cggtagccgg tccgcgcctg gcggagcagg tacggcgcga ggctcatgtt ctcggtaccg
      781 ccagcggcga cgatggacgc gtccccggcc ttgatggcct gcgccgcgtt cgcgacggcc
      841 cggagccccg agccgcagac catgttgacg gtgctcgcgg tgctctccac gggcaggccg
      901 gccttgatcg ccacctgcct ggcgacgttc tgcccgagcc cgccctggag cacgcagccg
      961 agcatcagct cgtcgatatc ggcgggcttg agccccgcgc gctcgagcgc cgcggccagc
     1021 gccgtgcgac cgagctccac cgggcttacc ccggccaacg ctccgttgaa gctgcctacc
     1081 gccgtcctcg cggcgcctac gatgaatgcg tctttca
//
LOCUS       DAOOLN010000934         1116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_176244_length_1116_cov_3.693685, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000934 DAOOLN010000000
VERSION     DAOOLN010000934.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176244_length_1116_cov_3.693685"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="PLW80_10945"
     CDS             complement(<1..920)
                     /locus_tag="PLW80_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67074.1"
                     /translation="MTANIASRRVARGMAAVIALLAYQAATAQDYPAVFAGLYDEALY
                     PEYDYEDHPTAISSLPGAFTVIDGNAYFDYSDTRATRLDDSVGVAGGSDLNRAIGLGG
                     DLSLALAKPRTGGTYLLGFDFSGDLSSAYELLDRYDSPTQYKLTQLYDWPLEFGLKGL
                     LANKGKSSSSGYSLDYDFSYTPNSYRWVTDTSQEPLLKYADAVSNDADVMSHAATLRG
                     DWSGKLGVFAVSAAAGYRFGLDDRSRKYLAVDTDGDDLDDSVMLYKDWSALTEASGGP
                     QYTTTQFDQKDLAFSHRVDLDVLARLDLSK"
     gene            complement(910..>1116)
                     /locus_tag="PLW80_10950"
     CDS             complement(910..>1116)
                     /locus_tag="PLW80_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67075.1"
                     /translation="YDIDRDGVTYGVAEELVYHTGETGEADEESASMHARWSRALSGI
                     GRGSAISSIINNPIVRPLRSNHDR"
BASE COUNT          177 a          340 c          401 g          198 t
ORIGIN      
        1 ttcttggaca ggtcgagcct ggcgaggacg tcgaggtcga cgcggtgcga gaacgccagg
       61 tccttctggt cgaactgggt cgtcgtgtac tgggggccgc ctgaggcctc ggtcagggcg
      121 gaccagtcct tatacagcat caccgagtcg tccaggtcgt cgccgtcggt atcgacggcc
      181 aggtacttgc ggctgcggtc gtcgaggccg aagcggtagc ccgctgccgc gcttacggcg
      241 aagacgccca gcttgccaga ccagtcgccg cggagcgtgg cggcgtgcga catgacgtcc
      301 gcgtcgttcg acacggcgtc ggcgtacttg agcagaggct cctggctcgt gtcggtcacc
      361 cagcggtagc tgttaggcgt atacgagaag tcgtagtcga gcgagtagcc cgacgacgat
      421 gacttgccct tattcgccag gagccccttg aggccgaact cgagcggcca gtcgtagagc
      481 tgggtcagct tgtactgggt cggcgagtcg tagcggtcga gcagttcgta cgcggacgaa
      541 aggtcgcccg agaagtcgaa gcccaggagg taggtgccgc cggtccgggg cttggcgagc
      601 gccaacgaca ggtctccgcc gaggcctatg gcccggttca ggtcggaacc gccggcgacg
      661 cctaccgagt cgtcgagcct cgtggccctg gtatccgagt aatcgaagta cgcgttgccg
      721 tcgatgacgg tgaaggcgcc cgggagcgag ctgatggccg taggatggtc ctcgtagtcg
      781 tactcggggt acagcgcctc gtcgtacagg ccggcgaaca cggccgggta gtcctgggcc
      841 gtcgcggcct ggtaggccag cagcgctatg acggcggcca tgccgcgggc cacgcgtcgt
      901 gaagctatgt tagcggtcat ggttgctcct caggggacgg acgatgggat tgtttatgat
      961 cgacgatatg gccgagccgc ggccgatccc ggacagcgcc cggctccagc gcgcgtgcat
     1021 cgaggccgac tcctcgtcgg cctcgccggt ctcgccggta tggtagacca gctcctcggc
     1081 caccccatag gtgacgccgt cgcggtctat gtcgta
//
LOCUS       DAOOLN010000935         1116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_176266_length_1116_cov_3.190386, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000935 DAOOLN010000000
VERSION     DAOOLN010000935.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176266_length_1116_cov_3.190386"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PLW80_10955"
     CDS             <1..415
                     /locus_tag="PLW80_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPB67076.1"
                     /translation="AAVLLELDPYALVDEKAPRVSPREPLSSIRRLLAERPAYLLAVV
                     DEAGVYLGDLRFDDIRPLLFDDASGAREAGEFATKDPKTLGLGLRPAEVLDSLDDARD
                     GALPVVDGRGTFLGYLWREAALNEYRRLMIAMTKD"
     gene            538..>1116
                     /locus_tag="PLW80_10960"
     CDS             538..>1116
                     /locus_tag="PLW80_10960"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPB67077.1"
                     /translation="MIDRNDQYDELRVLRLLFGISRVLDRTPDLRGALAPMLDLLESE
                     AGLGRGVVTLLDRSLGVLRIEAAPSLSDEERGQGLYRLGEGLIGRVFETGAPMTVRDL
                     SAEPRLLNRAGCRAPGELAGVSCHVVPIHSGDTVIGTLSAERKAVVRPPASQAEPDDA
                     TLEADRSLLEKVAELLADSAKLRERLQEDRIKV"
BASE COUNT          195 a          393 c          357 g          171 t
ORIGIN      
        1 cgccgcggta ctcctggagc tcgaccccta cgccctcgtc gacgagaagg cccccagggt
       61 atcgccccgg gaacccctat cgtcgattcg acgcttgctg gccgagcggc cggcctacct
      121 gctcgccgtg gtcgacgaag cgggcgtcta cctcggcgac ctccgcttcg acgatattcg
      181 ccccctgctc ttcgacgacg cctccggcgc tcgcgaggcc ggcgagttcg ctaccaagga
      241 cccgaagacg ctcgggctcg gcctgaggcc ggccgaggtg ctggacagcc tcgacgacgc
      301 ccgcgacggc gccctgcccg tcgtcgacgg ccgaggaaca ttcctcgggt acctatggcg
      361 cgaggcggcc ctgaacgagt accgccgcct gatgatcgcg atgacgaagg actgactcgc
      421 ctcgcatcga cggagatagc ctcgccgccg cccacggctt ctcccacctc gcgtcaatgg
      481 aagaagctag gacaacaggc attgaacttt ccaacatttt cgtagcatca tggcgtcgtg
      541 atcgatagaa acgaccaata cgacgaactc agggtactac ggctactctt cggcatctcg
      601 cgcgtgctcg accggacgcc cgacctgcgg ggcgcgctcg cgccgatgct cgacctactc
      661 gagtcggaag ccggcctcgg ccggggcgtc gtcaccctgc tcgaccgatc cctcggcgtg
      721 ctgcgtatcg aggccgcccc gagcctgagc gacgaagagc gcggccaggg cctctacagg
      781 ctcggcgagg gccttatcgg ccgtgtattc gagacgggtg ccccgatgac cgtacgagac
      841 ctgtcagccg agccgcggct actcaaccga gccggctgcc gagcgccggg cgagctggcc
      901 ggcgtttcat gccacgtcgt accgatacat tccggcgata cggtcatcgg cacgctgagc
      961 gctgagcgga aagcggtagt caggccgcct gcgagccagg ccgagcccga cgacgcgacg
     1021 ctcgaggcgg accgatcgct gctcgaaaag gtagccgagc tactggccga ttcggcgaaa
     1081 ctacgcgagc ggctccagga ggatcgcatc aaggta
//
LOCUS       DAOOLN010000936         1116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_176341_length_1116_cov_2.440151, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000936 DAOOLN010000000
VERSION     DAOOLN010000936.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176341_length_1116_cov_2.440151"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PLW80_10965"
     CDS             complement(<1..510)
                     /locus_tag="PLW80_10965"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HPB67078.1"
                     /translation="MNALQRSILPALLLGLAFATTLGAEEKTALSIMTAVYERPQPAD
                     MKATLTMTLVDSKGKERVRALEQRLASFGGVDKKIMMFQAPADVRGTSFMNWSYAEAG
                     KGDDQWIYMPALKRVKRITSDGKGGSFMGSDFSYDDLGARHPSRDDHVILGSETVAGE
                     ECWIIESTPK"
     gene            complement(513..>1116)
                     /locus_tag="PLW80_10970"
     CDS             complement(513..>1116)
                     /locus_tag="PLW80_10970"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPB67079.1"
                     /translation="RVIEYVDAQAADFDRFGITIRYAGSGYKSLIFSDLILQGQMSSL
                     GLSVIIVFMLVAFMFRSVLLGLVSTIPVLISIAVNFGVMGLLNVPLTTSTALISSIAV
                     GIGVDYAIHLITHYREALKALGDEERAARFAMSHTGRAVLLNAAIVISGFMVMVFSVF
                     PPNRQVGLLVSLNMLTAFLATVTIMFMVLRSIPPRLSKEL"
BASE COUNT          219 a          317 c          371 g          209 t
ORIGIN      
        1 tttgggcgtg ctctctatta tccagcattc ctcgccggcc acggtctcgc taccaaggat
       61 gacatgatcg tccctggaag gatgacgggc gcccaggtcg tcgtagctga aatccgagcc
      121 catgaaggac ccgcccttgc cgtcggaggt gatgcgcttg acccgcttga gggccggcat
      181 gtagatccac tggtcgtcgc ccttgcccgc ctcggcgtag ctccagttca tgaaggaggt
      241 gccgcggaca tcggcggggg cctggaacat catgatcttc ttgtcgacgc ccccgaaact
      301 ggccaggcgc tgttcgagcg cgcggacgcg ttccttgcct ttggaatcga cgagggtcat
      361 ggtcagggtg gccttcatgt cggccggctg gggccgttcg tacaccgccg tcatgatgga
      421 tagggccgtt ttctcttccg cgccgagcgt cgtcgcgaaa gccaggccga gcaacaaggc
      481 cggcaggatg gatcgttgca atgcgttcat ttctataact cctttgataa ccgtggaggg
      541 attgagcgaa ggaccatgaa catgatggtg acggtagcca ggaacgcggt gagcatgttc
      601 agcgacacga gaagccccac ctggcggttg ggcgggaaca ccgagaagac catgaccatg
      661 aagccggaga tgacgatggc cgcgttgagt agcaccgccc tgccggtatg gctcatcgcg
      721 aagcgggcgg cacgttcctc gtcgccgagg gccttcagcg cctcgcggta atgggttatc
      781 aggtggatgg cgtagtcgac gcctatgccc accgcgatgc tggatatgag ggccgtggag
      841 gtggtcaggg gcacgttgag gagccccatg acgccgaagt tgaccgctat cgatatgagc
      901 accggaatcg tggacacgag gcccaggagc acgctgcgga acatgaaggc cacgagcatg
      961 aagacgatga tcacggagag gccgaggctg ctcatctgcc cctgcagtat gaggtccgag
     1021 aagatcaggc tcttgtaacc ggatcccgcg tagcggatgg tgatgccgaa ccggtcaaag
     1081 tcagccgcct gggcatcgac gtactcgatg acgcgc
//
LOCUS       DAOOLN010000937         1112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_177399_length_1112_cov_1.980132, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000937 DAOOLN010000000
VERSION     DAOOLN010000937.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177399_length_1112_cov_1.980132"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLW80_10975"
     CDS             complement(<1..234)
                     /locus_tag="PLW80_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67080.1"
                     /translation="MTTKANSPARPCHGPLSSIESAYERTSRARHADSPHPTGAGLLV
                     LALGGRALLVEGRRGFAEPREAARLIHRFRIGPR"
     gene            complement(215..664)
                     /locus_tag="PLW80_10980"
     CDS             complement(215..664)
                     /locus_tag="PLW80_10980"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HPB67081.1"
                     /translation="MKKPLATLGVVISSIVAIVGIAGLFTPKLPGLPLILFAAIGLMH
                     TSPDFVSRFNRTPRESSYIDDLLRERSMTRRNKIRTLSVTSLLLIVLFVITDSVVARI
                     LYAVFAVGKYWFFYFYIWTKVEDGHGCPDGVDCPDDKGNAGGDDEGE"
     gene            complement(820..>1112)
                     /locus_tag="PLW80_10985"
     CDS             complement(820..>1112)
                     /locus_tag="PLW80_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPB67082.1"
                     /translation="LALPGYGWLKERYPEPVLRAAAALGPIARELGGTPAQLAIAWCA
                     RNPNVSTVITGASRIAQLEENLGALDLVPKLSAEVLARIDEAMAPATAAMRD"
BASE COUNT          190 a          344 c          386 g          192 t
ORIGIN      
        1 ccgggggccg atgcggaaac ggtggatcag gcgggccgcc tcgcggggct ccgcgaagcc
       61 ccggcgcccc tcgacgagga gcgcgcgccc gcctagcgcc aggaccagga gcccggcgcc
      121 ggtgggatga ggcgagtccg catgacgggc gcgcgaagtt cgttcatacg ccgattctat
      181 cgacgatagc gggccgtggc aaggacgggc ggggctattc gccttcgtcg tcaccgcccg
      241 cgttgccctt gtcgtccggg cagtcgacgc cgtccgggca gccgtgcccg tcctcgacct
      301 tggtccagat gtagaagtag aagaaccagt acttgcccac ggcgaagacg gcgtagagta
      361 tcctggccac gaccgagtcg gtgatcacga acaggacgat gaggagcagc gaggtgacgg
      421 acagcgtacg gatcttgttg cgccgcgtca tcgaccgttc gcgtaggagg tcgtcgatgt
      481 acgacgactc cctgggcgtc cggttgaagc gcgatacgaa gtccggtgag gtgtgcatga
      541 ggcctatggc cgcgaacagg ataagcggaa ggcccggtag cttcggcgtg aataggcccg
      601 cgatgcctac gatggctact atcgagctga tgacgacccc tagggtggcc agcggcttct
      661 tcatgggtat cctcaaggat cgggttttta cagtattaat tcaagggtac cgccattata
      721 tagcatggct ttggccagcg ggataggggg aaaacggttg aggcgacgat attgttggac
      781 aaaaccggcg atttcccacc cctatcccgc caacggctat cagtcgcgca tcgcggcggt
      841 tgccggcgcc atcgcctcgt ctatcctggc cagcacctcg gcgctcagct tgggcaccag
      901 atcgagcgcg ccgaggttct cctcgagctg ggctatgcgg ctcgccccgg ttatgaccgt
      961 gctgacgttc gggttgcggg cgcaccaggc tatcgccagc tgggccgggg tgcctccgag
     1021 ctcgcgcgct atcggcccga gcgccgccgc cgcgcgcagc accggttcgg ggtagcgctc
     1081 cttgagccag ccgtagccgg gcagggccag gc
//
LOCUS       DAOOLN010000938         1109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_178096_length_1109_cov_2.742884, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000938 DAOOLN010000000
VERSION     DAOOLN010000938.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178096_length_1109_cov_2.742884"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..722)
                     /locus_tag="PLW80_10990"
     CDS             complement(165..722)
                     /locus_tag="PLW80_10990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPB67083.1"
                     /translation="MTPSGSEAADEDIIARVLDGDADAFGALVRRYQRRILRLGYGFF
                     KDADEAEDFAQDVFVKAYVGLAGFKGRSSFSTWLTRIAYNAGINAKRKAGRYEPLDSE
                     PVDSRTLSPEDAHIRAETAEALRAAMAALPEKYAVCLDLYFREGMRYEDISASTGFPV
                     NTIKSHVFRAKRDLRKALGAEEGPL"
     gene            complement(719..>1109)
                     /locus_tag="PLW80_10995"
     CDS             complement(719..>1109)
                     /locus_tag="PLW80_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67084.1"
                     /translation="QCPGGPDSVYLRYGALMENGVSAASKVIISIIPIVGIVMGCTVV
                     FFYILWSHREKMLMIEKGSYAPKPLDVDVFSLLAGILLVAVGITLTAVFLVMDTAGYT
                     LLGGLIPLSVGLGFLAFYSLRARRRAT"
BASE COUNT          193 a          365 c          352 g          199 t
ORIGIN      
        1 gtcggccgag ccggccaggt cgggcaggcg gtataggctg agcgccgcct tgacggcggc
       61 ggcctcggcc gcgcactcgg ggcaggagcg cgcgtggacg agcatgtcga tcgtcatgtc
      121 ctcgccgtcg agcgcgtcga gctggttcct gaagctcctg caattcatag cggcccctcc
      181 tcggcgccga gcgcctttct caagtcccgc ttggccctga acacgtgcga cttgatcgtg
      241 ttgaccggaa agccggtcga cgcgcttata tcctcgtagc gcataccctc cctgaagtac
      301 aggtccaggc agacggcata tttctccggc agggcggcca tggcggccct gagcgcctcc
      361 gcggtctcgg cgcggatgtg ggcatcctcg ggagatagcg tcctcgaatc caccggctcc
      421 gagtccaatg gctcgtagcg cccggccttg cgcttggcgt tgatgccggc gttgtaggcg
      481 atgcgcgtca gccaggtcga gaagctggag cgccccttaa agccggccag gccgacgtaa
      541 gccttgacga acacgtcctg ggcgaagtcc tcggcctcgt cggcgtcttt gaagaagccg
      601 tacccgaggc gcagtattct ccgctggtac ctgcgcacca gcgcgccgaa cgcgtccgcg
      661 tccccgtcga gcactctggc gatgatatcc tcgtcggcgg cttccgatcc gctcggcgtc
      721 atgtcgcccg tcggcgggcg cgtagcgagt agaaggccag aaagccgagg ccgaccgaaa
      781 ggggtatgag tcccccgagc agggtgtagc cggcggtgtc catgaccagg aagaccgccg
      841 tcagggttat cccgactgcc acgaggagta tccccgccag caggctgaac acgtcgacgt
      901 cgagcggctt gggcgcgtaa ctgcccttct cgatcatcaa catcttctcc ctatggctcc
      961 ataggatgta aaagaacacg acggtgcaac ccatcacgat acccacgatg gggatgatcg
     1021 agatgatcac cttggacgcc gccgacactc cgttttccat aagcgctccg tatcgcaagt
     1081 atacactatc gggcccgccg gggcactgt
//
LOCUS       DAOOLN010000939         1108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_178344_length_1108_cov_3.211776, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000939 DAOOLN010000000
VERSION     DAOOLN010000939.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178344_length_1108_cov_3.211776"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..>1108)
                     /locus_tag="PLW80_11000"
     CDS             complement(113..>1108)
                     /locus_tag="PLW80_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HPB67085.1"
                     /translation="RAAAAIAGNDHRLGANEAPPAIVSIFFGSLLSELLDSIADGKPL
                     AGTGSAHRIELGVTTIPALPVDISDRNRTSPFAFTGNKFEFRMVGSSQSIATPNTYLN
                     TAVAQVLSEFADALEAAPDRLSAIQDIIRASYSSHRRVVFNGNGYSDEWIKEAERRGL
                     PNVRSTVDALAILAREETIGLFETHKVLSRAESVSRYHIYLEKYSKQVNIEAGVMADM
                     ARRTIFPATGAYAAALARDAAALAAIGAASAAIEKRAKRIAELAGDIAGDTERLEAIL
                     GEAQGIDDVGLRAKAYRDGVVPQMGALRGRVDAAEKLVDKKAWPLPTYEELLFKL"
BASE COUNT          167 a          365 c          378 g          198 t
ORIGIN      
        1 ggagcattcc gacgacgacc gcggcggagc tggccgacgc ggccggcgtg accatcgtcg
       61 gccgcatcgg cgaccagtcg ccggtcgtct atactcgccc cgagcgcgtc gtctaaagct
      121 tgaacagcag ctcctcgtag gtcggcaagg gccacgcttt cttgtcgacc agcttctcgg
      181 cagcatcgac tctgccgcgc agggctccca tctgcgggac gactccgtcg cggtaggcct
      241 tggcccgcag gccgacgtcg tcgatcccct gggcctcgcc gagtatcgct tcgaggcgct
      301 cggtatcgcc ggctatatcg ccggcgagct ccgctatgcg cttggcccgc ttctcgatcg
      361 ccgccgacgc ggccccgatc gcggcgaggg cggcggcatc gcgggccaga gccgccgcgt
      421 aggcgccggt agccgggaag atcgtacgcc tggccatgtc ggccatcact ccggcctcga
      481 tattgacctg cttcgagtac ttctcgagat agatatggta gcggctgacg gactccgccc
      541 tcgacagcac cttgtgcgtc tcgaacagcc ctatcgtctc ctcgcgcgcg agtatcgcca
      601 gcgcgtcgac cgtcgagcgg acgttcggca gccctcggcg ctcggcttcc ttgatccatt
      661 cgtccgaata gccgttgccg ttgaacacga ccctgcggtg cgacgaatag gaagccctga
      721 tgatgtcctg tatagccgag agccggtcgg gggccgcctc gagcgcgtcg gcgaactcgg
      781 acaggacctg ggcgaccgcg gtgttcagat aggtgttcgg cgtggctatg gactgggaag
      841 agccgaccat gcggaactcg aatttattgc cggtgaaggc gaaggggctg gtccggttgc
      901 ggtcggatat gtcgacaggc agggcaggga tcgtggtcac gccgagctct atccgatgcg
      961 ccgagccggt cccggcgagc ggcttgccgt cggctatgct gtcgagcagc tcggagagga
     1021 gactgccgaa gaatatcgat acgatcgccg gcggcgcctc gttggcgccg agccggtggt
     1081 cgttgcccgc tatcgcggcc gccgcgcg
//
LOCUS       DAOOLN010000940         1108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_178493_length_1108_cov_1.849953, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000940 DAOOLN010000000
VERSION     DAOOLN010000940.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178493_length_1108_cov_1.849953"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..527)
                     /locus_tag="PLW80_11005"
     CDS             complement(114..527)
                     /locus_tag="PLW80_11005"
                     /inference="COORDINATES: protein motif:HMM:NF012430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPB67086.1"
                     /translation="MPRAVDGTRRKDHRKKILKLAKGYYGRRSTNHKTAKDAVAKATR
                     SYLEAHDKIPRGLAVSAEEIREGLVALVSVFLADPQFQGQTKDRLNNPDVKPAIDGVL
                     RAALETWFHTRPPSARPSRPGWSRPPGRGPRPATP"
     gene            complement(663..863)
                     /gene="rpmI"
                     /locus_tag="PLW80_11010"
     CDS             complement(663..863)
                     /gene="rpmI"
                     /locus_tag="PLW80_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPB67087.1"
                     /translation="MPKMKTKRAASKRYTLSADGKVKYKKMNLRHILTKKSSKRKRKL
                     RKAGFVASGPVYQIKKKLLPYG"
     gene            complement(877..>1108)
                     /locus_tag="PLW80_11015"
     CDS             complement(877..>1108)
                     /locus_tag="PLW80_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-3 C-terminal
                     domain-containing protein"
                     /protein_id="HPB67088.1"
                     /translation="KVKVTIRFRGRELAHTEIGEEVLKSVLTKIEGIYAMESPPRMEG
                     RFMSMMLIPKAPAIKKPEPQRPQPDAAPDTKA"
BASE COUNT          150 a          354 c          363 g          241 t
ORIGIN      
        1 cccctcgacc aggaagagct cgcgccgcat gggctccggg gaggagcagt ccgcgagctt
       61 gccggggagc tgcacccgcc gggtgatgcc ggtgctcttg ccccgcacct tgctcacggc
      121 gttgcgggcc gcggcccgcg cccgggcggc ctcgaccacc cgggccgtga cggcctcgcc
      181 gacggagggc gggtgtggaa ccaggtctcc agcgcggcgc gcagcacgcc gtcgatggcc
      241 ggcttgacgt ccgggttgtt gagccggtcc ttggtctggc cctggaactg cggatccgcg
      301 aggaagacgc tgaccagggc gaccagcccc tcccggatct cctcggcgga gacggccagg
      361 ccgcggggga tcttgtcgtg ggcctcgagg tagctgcgcg tcgccttggc gaccgcgtcc
      421 ttcgcggtct tatggttggt ggacctgcgt ccgtagtatc ctttggcaag cttgaggatc
      481 ttcttgcggt gatcctttcg cctggtgccg tcaactgctc tgggcattgc tattctcctt
      541 ggaaaatatg aaccggccgt cggggaaagg tcctcgtcga ccggcgcgcc gtagcggcgc
      601 cgagcctcat cctatcgtcg agtccgccgg cggccctaac gggggaaagg ccgcttgtcg
      661 gattaaccgt aggggagaag cttcttcttg atctgatata cggggccgga ggccacgaag
      721 ccggccttgc gtagcttgcg cttgcgcttc gaactcttct tggtgaggat atggcgaagg
      781 ttcatcttct tgtacttgac cttgccgtcc gcgctcagcg tatagcgttt agaggcagcc
      841 ctcttcgtct tcatcttagg cattacgatc catccttcag gctttagtat ccggcgcggc
      901 gtccggctgg ggccgctgcg gctccggctt cttgatcgcc ggggccttcg gtataagcat
      961 catcgacatg aagcgccctt ccatcctcgg cgggctctcc atggcgtata ttccctcgat
     1021 cttggtcagt acgctcttca ggacttcctc gccgatctcg gtatgggcga gctcgcggcc
     1081 cctgaagcgt atggtcacct tgaccttg
//
LOCUS       DAOOLN010000941         1105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_179081_length_1105_cov_3.842857, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000941 DAOOLN010000000
VERSION     DAOOLN010000941.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179081_length_1105_cov_3.842857"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1105)
                     /locus_tag="PLW80_11020"
     CDS             complement(<1..>1105)
                     /locus_tag="PLW80_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPB67089.1"
                     /translation="GALITIDNATGHIKAIVGGSKFEQSNQLIRATQAQLMPGSCFKP
                     LYYSAAIDSRRFTPGTLIYDAPVVFYNEDGTPYIPLNYKGEWQGQVLTWNALAKSMNV
                     PAVKVLDGIGFDAAINRAAALLDITDPEQVRTTFPRVYPLALGISAVTPLRMARAFAV
                     FANQGREVTPIAIRSIEDRDGRPLLDPEKDLRAEQKKKGSGIQVVSPQNAYVMTDMLQ
                     RVVKVGTLAWPTSSGAKFTYRDADGKRYTIPAAGKTGTTQNWADAWTVGFTPYMTTAI
                     WFGFDRPGNSLGVSQSGAAIAGIAWADYMAEIHRGLPAKDFIRPQSGLIDVTVCSVSG
                     LLPTEYCDEGTLTLTYYEGTQPKSYCDLHEFKSE"
BASE COUNT          180 a          324 c          382 g          219 t
ORIGIN      
        1 gctcggattt gaactcgtgc aggtcgcagt agctcttggg ctgagtgccc tcgtagtagg
       61 tgagcgtcag ggtgccctcg tcgcaatatt cagtcggcaa gaggccggag acggagcaga
      121 cggtgacgtc gatgaggccg ctctggggtc ggatgaaatc cttggctggc agcccccggt
      181 gtatctcggc catgtagtcg gcccaggcta tgcccgcgat ggcggcgccg gactggctga
      241 cgccgagcga gttgcccggc ctgtcgaagc cgaaccagat cgccgtcgtc atgtacggcg
      301 tgaagcccac cgtccaggcg tccgcccagt tctgggtcgt gccggtcttg cctgctgcgg
      361 gtatggtata gcgcttgccg tcggcgtcgc gataggtgaa cttggctccg gacgaggtcg
      421 gccaggccag ggtaccgacc ttcacgacgc gctgtagcat gtcggtcatc acgtaggcgt
      481 tctgcgggga gacgacctgt atgccggagc ccttcttctt ttgctccgcg cgcagatcct
      541 tctcagggtc gaggaggggc cgtccgtccc tgtcctcgat cgaccgtatg gcaatcggcg
      601 tgacctcgcg gccctgattg gcgaatactg cgaaggccct ggccatgcgt agcggcgtta
      661 cggcgctaat accgagggcc aacggataga cccgcgggaa ggtggtacga acctgctccg
      721 ggtcggtaat atcgagcagg gcggccgcgc ggtttatggc cgcatcgaag ccgatgccgt
      781 cgagcacctt gacggccggc acgttcatgg acttggccag cgcgttccag gtaagcacct
      841 ggccctgcca ctcgcccttg tagttgagcg ggatgtacgg cgtaccgtcc tcgttataga
      901 agactaccgg cgcgtcgtag atcaaggtgc cgggcgtgaa gcgcctcgag tcgatcgcag
      961 cggagtaata gagaggcttg aagcaggagc cgggcatcag ctgggcctgg gtcgcgcgta
     1021 tcagctggtt ggactgctcg aactttgagc cgccgactat cgccttgata tgcccggtcg
     1081 cgttgtcgat ggtgatgagc gcgcc
//
LOCUS       DAOOLN010000942         1105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_179117_length_1105_cov_3.080000, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000942 DAOOLN010000000
VERSION     DAOOLN010000942.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179117_length_1105_cov_3.080000"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..1030
                     /locus_tag="PLW80_11025"
     CDS             137..1030
                     /locus_tag="PLW80_11025"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPB67090.1"
                     /translation="MRADLHIHSRFSDGGHWPSDIAGLAAAAGLEAVCLTDHDTFGGC
                     VELAEAAGQLGLASWPAVEIDCEDETLGYKSELLAYFPGGSYELTASMLAVSRAERAA
                     RVEAMFARAAALFAAPELSFGRWVEARLAGRGPGSPRVDLGTLRFAKTDLFLALKRAG
                     RVSADVDYREFKKAYFDTGLFSDLRFAKPSLETVAESVSRDGGTLVVPHIGHEFGDSI
                     ASMRAGTRRLDAMLSRFRDLGVRGVELYYYRNDDREAMNALVTERCERFGFFHTYGSD
                     CHGPDSNKNGIGSFYGDFRGF"
BASE COUNT          166 a          356 c          372 g          211 t
ORIGIN      
        1 ttctccatcg agaacagcat ttcctctaag atcgcgtcgt ctaggtcgaa cattctgtcc
       61 tccggtcggg ggcgcggcgt tcgtcggctt gtccgccgcg ctcctcgcgg gttagtatac
      121 tacccagggt accacgatgc gagccgatct ccatatacat tcgcgcttct ccgacggcgg
      181 ccattggccg tcggacatag ccgggctcgc cgccgcggcg ggactcgagg ccgtctgcct
      241 gaccgatcac gacaccttcg gcggctgcgt cgaactggcc gaggcggccg gacagcttgg
      301 tctggccagt tggcccgccg tcgagattga ttgcgaggac gagacgcttg gctataagag
      361 cgagctgctc gcctatttcc cgggcggttc ctacgagctc acggcctcga tgctcgctgt
      421 gtcgcgagct gagcgggccg cgcgcgtcga ggctatgttc gcccgcgccg ccgccctgtt
      481 cgcggctccg gagctgtcgt tcgggcgctg ggtcgaggcg cgcctggccg gccgcggccc
      541 cgggtcgccg cgtgtcgacc tcggcacgct gcgcttcgcc aagacggacc tgttcctggc
      601 gctcaagcgg gccggccgcg tgtcggccga cgtagactat cgagaattta aaaaggccta
      661 cttcgatacc gggttgttct ccgatctgcg cttcgccaag ccgagcctcg agacggtggc
      721 cgagtcggtc tccagggacg gcggcacgct cgtcgtaccg catatcgggc acgagttcgg
      781 cgactcgatc gcgtcgatgc gcgccggcac gagacggctg gacgccatgc tgtcgcgctt
      841 ccgggacctt ggcgtacgcg gcgtcgagct gtactactat cggaacgacg accgggaggc
      901 gatgaacgcg ttggtcaccg agcgctgcga gcgcttcggc ttcttccaca cgtacggctc
      961 cgattgccac gggccggatt cgaacaagaa cgggatcggt tcgttctacg gcgactttag
     1021 gggattctag gtctagcggg ccgcgattag ccgaaaggac aagacaatgg cgattgagtg
     1081 gcctacccgg gcggcggaag tgctc
//
LOCUS       DAOOLN010000943         1105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_179267_length_1105_cov_1.848571, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000943 DAOOLN010000000
VERSION     DAOOLN010000943.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179267_length_1105_cov_1.848571"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="PLW80_11030"
     CDS             <1..870
                     /locus_tag="PLW80_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic filament protein CfpA"
                     /protein_id="HPB67091.1"
                     /translation="QLIEERKAYDVQITSFETEVQHLWDVLEMMYEDSKFLKRITDFD
                     DLAKLMKSKIRKRIKEKSGEPLYEDIAKVWDEIAFVKPAETDVESLNCTYIYEKDKVK
                     NRLILMRDKLKNMYAYQYPIQRRVVEDRLTFLEKEFQRFDYMINPYHIQPGLLLDVDI
                     CSIKRKKATLDGMANVLNEFLHGVSKGFQDAAFASFSRRRSTVREDIAQSFAPAQIED
                     APESASAGYLAMLNAEPESPALPEATMAIAAKKRGGRPKGSGVVDATPKKAGAKRGPK
                     PRSKKDEGGIREL"
BASE COUNT          256 a          343 c          334 g          172 t
ORIGIN      
        1 cagctgatcg aggagcgcaa ggcctacgac gtacagatca ccagcttcga gaccgaggtg
       61 cagcacctct gggacgtgct cgagatgatg tacgaggact ccaagttcct caagcgcatc
      121 accgatttcg acgatctggc caagctcatg aagagcaaaa tccgtaagcg gatcaaggag
      181 aagtctggcg agcccctcta cgaggatatc gccaaggttt gggacgagat cgccttcgtc
      241 aagcctgccg agactgacgt cgagagcctg aactgcacct acatatacga gaaggacaag
      301 gtaaagaacc gccttatcct gatgcgcgac aagctcaaga acatgtacgc ctaccagtac
      361 ccgatccaac gccgcgtcgt cgaggacagg ctgaccttcc tcgagaagga attccagcgc
      421 ttcgactaca tgatcaaccc gtaccacatc caaccgggcc tcctcctgga cgtggatatc
      481 tgctcgatca agcgcaagaa ggccacgctc gacggcatgg ccaacgtact caacgagttc
      541 ctgcacggcg tgtccaaggg cttccaggac gctgcattcg cctcgttcag ccgcaggcgc
      601 tccaccgtcc gagaggacat cgcccagagc ttcgccccgg cgcagatcga ggacgctccc
      661 gagtcggctt cggctggcta cctggccatg ctcaatgccg agcccgagtc cccggccctg
      721 ccggaggcga ccatggctat cgcagccaag aagcgcggcg ggcggcctaa gggaagcggg
      781 gtcgtcgacg ctacccccaa gaaggctggc gccaagcggg gacccaagcc gcgatccaag
      841 aaggatgagg gcggcatccg cgaactctaa gcgagcgtag cgaacgcgcc ccccggtctt
      901 cggggggcgc ttttcattaa ggaaggggaa agcatgattg cctcgagctg caagtacgag
      961 tccttgtcat ccagggaaac gttcaacgcg tcggtccgtc acgtcaaggc gacggtagcg
     1021 ctggccgaga gcttagcggg aggcgagaac ctggccgacg ctctcgacgc catgatagcc
     1081 gacaaggcag tgggcggcga cgaga
//
LOCUS       DAOOLN010000944         1105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_179291_length_1105_cov_1.645714, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000944 DAOOLN010000000
VERSION     DAOOLN010000944.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179291_length_1105_cov_1.645714"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PLW80_11035"
     CDS             complement(<1..478)
                     /locus_tag="PLW80_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67092.1"
                     /translation="MKGLRRFLGFALYLCMAFPLMLGGLAVASLAPAAGDSAALKTLV
                     VDPRFSELLRSPDLVTMAPETMRLGGSVLNGKAATAAFQASVPTSAVVDTVSGAIDAA
                     YEAFGRGDGFFYFDARPLKAAVSTGAPTFASVYVAKAALPETVTRAPGDPGRAETPI"
     gene            complement(475..>1105)
                     /locus_tag="PLW80_11040"
     CDS             complement(475..>1105)
                     /locus_tag="PLW80_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB67093.1"
                     /translation="SLVLPFAGYESPAGIDRRDEEEAMRLLAQAGHPGGEGLPALRFV
                     YYESETHEANLAILEEAWAAVGVRVERVRVPSGDAIRDYRQEGYELSFTSWIGDFADP
                     AAFLLMWTADSGLNEGGYRSREYDALMARSMEEEGKARLATLAQAEAKLLSDAPLLPL
                     YHSVSFNVIDLDAVTGWFQNPLDIHPFKSMGFGAPKARPYVALAGGTAR"
BASE COUNT          176 a          358 c          408 g          163 t
ORIGIN      
        1 tgatcggcgt ctccgcccga cccggatcgc ccggagccct cgtcacggtt tccggtagcg
       61 cggccttggc gacgtagacc gaggcgaatg tcggcgcgcc ggtactgacg gccgccttga
      121 gcggcctcgc gtcgaagtag aagaaaccgt cgccgcggcc gaaagcctcg taggcagcgt
      181 cgatcgcgcc cgagacggtg tcgacgacgg cgctcgtcgg cacggaggcc tggaacgccg
      241 cggtcgcggc cttgccgttc agcacggagc cgccgaggcg catggtctcg ggcgccatcg
      301 tcaccaggtc cggcgatcgt agtagctcgg agaagcgcgg gtcgacgacc agggtcttga
      361 gcgcggcgct gtcgccggcc gccggcgcga gcgaggctac cgccaggccg ccgagcatca
      421 gcgggaaagc catgcagagg tagagggcga agcccaggaa gcgtctgagt cccttcatcg
      481 ggcggtacct ccggcgagag cgacgtaggg cctggccttg ggggcgccga agcccatgga
      541 cttgaacggg tggatatcga gcgggttctg aaaccagccg gtgacggcgt cgaggtcgat
      601 gacgttgaac gataccgagt ggtagagcgg taagagggga gcgtcggaca gtagcttggc
      661 ctccgcctgg gcaagcgtcg cgagtcgggc cttgccctcc tcttccatcg agcgggccat
      721 cagcgcgtcg tactcgcggc tgcggtagcc gccctcgttc aagccggagt cggcggtcca
      781 catgaggagg aaggccgccg ggtcggcgaa gtcgcctatc cagctcgtga aggacagctc
      841 gtagccttcc tgcctgtagt cgcgtatcgc gtcgccggag ggaacccgca cgcgctcgac
      901 gcgtacgccg accgcggccc atgcctcctc gagtatggcg aggttcgcct cgtgggtctc
      961 cgactcgtag tagacgaagc gtagcgcggg caggccctcg ccgccgggat ggcccgcctg
     1021 ggcaaggagg cgcatcgcct cctcctcgtc tcggcggtcg atgccggcag gcgactcgta
     1081 gccggcgaag ggcagcacga gcgag
//
LOCUS       DAOOLN010000945         1104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_179455_length_1104_cov_2.286940, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000945 DAOOLN010000000
VERSION     DAOOLN010000945.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179455_length_1104_cov_2.286940"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..>1104)
                     /locus_tag="PLW80_11045"
     CDS             complement(19..>1104)
                     /locus_tag="PLW80_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67094.1"
                     /translation="AGLAELEQRYGFSSTLSNQRTRRAEIAELSVDFEPEVLEYALLP
                     CPDGPGWGVNVNAAMLAGAGGLLVSCDDDVFCRPARPATADDSDDPRFSMSTEYQVGE
                     LAYHDSRESLLSSMRPVDVDIVGRYRAVLGMSAGELAGEGAWRDDDAPVMVASPGMYG
                     DSAMGGSRTALSLVGAARERLMAGPYERLRLSRELVRVAPRPTLSPGTQLIMTQTGFD
                     DRSAPPPFLPFGRNPDGLSAVLMRLVYPASLTAYLDFGLLHAPPEPRVAVRADLTSYR
                     VNLAELVMALAIANRPAASVADPAERFRRIAAALVDAAAFGNSALVDALHGSWAPQLG
                     RYVERLEVMLDTHDREPAAWAADSMPL"
BASE COUNT          184 a          372 c          408 g          140 t
ORIGIN      
        1 cccgaccaag gccgagaact acaagggcat cgagtcggcg gcccacgcgg ccggctcgcg
       61 gtcgtgggta tcgagcatga cctcgagacg ctcgacgtag cggccgagct ggggcgccca
      121 ggatccgtgc agggcgtcca cgagggcgga gttaccaaag gcggccgcgt cgacgagggc
      181 cgcggcgata cgcctgaagc gctcggccgg gtcggcgacc gaggccgccg ggcgattggc
      241 tatcgcgagc gccatgacga gctcggccag gttgacgcga tacgacgtca ggtcggcgcg
      301 gacggcgacg cgcggctcgg gcggcgcgtg gaggagaccg aagtcaagat aggccgtcag
      361 gctggccgga tagacgaggc gcatgagcac ggcggacagg ccgtcggggt tgcgtccgaa
      421 gggcaggaag ggcggcggcg ccgagcggtc gtcgaagccg gtctgcgtca tgatcagctg
      481 agtacccgga gacagggtcg gccgcggggc cacgcggact agctcgcgcg acaggcgtag
      541 ccgctcgtag ggaccggcca tcaagcgctc cctggccgcc ccgacgagcg acagcgcggt
      601 acgggagccg cccatcgccg agtcgccgta catgcccggg ctcgctacca tgaccggcgc
      661 gtcgtcgtcc cgccacgcgc cctcgcccgc cagctcaccg gcgctcatcc ccaataccgc
      721 gcggtatcgg ccgacgatgt ccacgtcgac cgggcgcatc gacgacagga gggattcacg
      781 gctatcgtgg taggctagct cgccgacctg atactcggta gacatggaga agcgcgggtc
      841 gtcggagtcg tccgccgtgg ccggtctggc cggccggcag aacacgtcgt cgtcgcacga
      901 cacgaggagc cctccggcgc ccgcgagcat ggcggcgttc acgttgacgc cccatccggg
      961 gccgtcaggg catggcaaga gggcgtactc gagcacctcg ggttcgaagt cgacagacaa
     1021 ttccgctatc tcggcgcgcc tcgtcctctg gttcgacagg gtcgacgaga aaccgtagcg
     1081 ctgctccagc tcggcgagcc ccgc
//
LOCUS       DAOOLN010000946         1103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_179685_length_1103_cov_2.419847, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000946 DAOOLN010000000
VERSION     DAOOLN010000946.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179685_length_1103_cov_2.419847"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PLW80_11050"
     CDS             <1..306
                     /locus_tag="PLW80_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67095.1"
                     /translation="DYIRKAKKAGFVASAMALYRVVRSRIVIVSATAATTVVGAIPLL
                     VLDTGDMGMVRALAFVTACGGAASFMAAVTVVPALAAGAPRLFDRFGPSAPPRDGGD"
     gene            299..>1103
                     /locus_tag="PLW80_11055"
     CDS             299..>1103
                     /locus_tag="PLW80_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67096.1"
                     /translation="MTSMRLKSSFSVLSTTVAMYVMAFAMAPRVARADEPPVIGFDTV
                     SYEHAVAMADYEQGSGRWMLVARTKADRELARWELLASESYPDDRDVARARADLADWS
                     DEYIAERYAAWLEDRFFGEESGRLRGLVSEAASRANRAFLYVCDDDGMVSFDDAGDPI
                     MLARGADGTIASDKEAWLEMTESSMREALARFDLDAEASLNAYLPELSAYIGPDLSRY
                     ESIAASAATSARDRVVSELDALIAREERLFVARREADSWSLRKRSDGEAA"
BASE COUNT          190 a          340 c          370 g          203 t
ORIGIN      
        1 gactatatac gaaaggccaa aaaagccggt ttcgtcgcaa gcgccatggc tctctaccgc
       61 gtagtgcgtt cgaggatagt catcgtatcg gcgaccgcgg ctacgaccgt cgttggagcg
      121 atacctctcc tcgtcctcga tacaggcgac atggggatgg tcagggcgtt agccttcgtg
      181 accgcctgcg gcggagcggc ttcctttatg gccgccgtta ccgtcgtacc ggccctggcg
      241 gcgggggcgc cgcgcctgtt cgacagattc gggccttcgg ccccgccacg cgatggaggt
      301 gactagtatg cggctcaagt cgtcgttcag cgtcttgtcg accacggttg ccatgtacgt
      361 catggccttc gccatggcgc ctcgcgtcgc tcgcgccgac gagccgccgg tgatcggctt
      421 cgacacggta tcgtatgaac acgcggtcgc catggccgat tacgaacagg gctccgggcg
      481 atggatgctc gtcgccagga cgaaggccga cagggagctc gcacgttggg agctactcgc
      541 gtccgagtcg tacccggacg atcgcgacgt ggcccgcgcc cgagccgacc tcgccgattg
      601 gagcgacgag tatatcgccg agcgttacgc cgcatggctc gaggatagat tcttcggcga
      661 ggaatcgggg cgcctacgtg ggctcgtgtc ggaggcggcc tccagggcga atcgcgcgtt
      721 cctatatgta tgcgatgacg acggaatggt gtcgttcgac gatgccggcg accctattat
      781 gctggctcgc ggcgccgacg ggactatagc ctcggacaag gaggcttggc tcgagatgac
      841 cgagtcctcc atgcgcgagg ctctagcgcg gttcgacttg gacgccgagg caagcctaaa
      901 cgcgtacttg ccagaactct cggcgtatat tggcccagac ctttcgcgat atgagtcgat
      961 agccgcctcc gccgcaacca gcgcccgcga cagggtagtt agcgagctcg acgccttgat
     1021 cgcccgcgag gagcgcctgt tcgtcgcaag aagggaagcg gactcgtgga gcctccggaa
     1081 gcggagtgac ggcgaggccg ccg
//
LOCUS       DAOOLN010000947         1102 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_180066_length_1102_cov_1.644699, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000947 DAOOLN010000000
VERSION     DAOOLN010000947.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180066_length_1102_cov_1.644699"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLW80_11060"
     CDS             <1..180
                     /locus_tag="PLW80_11060"
                     /inference="COORDINATES: protein motif:HMM:NF020940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HPB67097.1"
                     /translation="FPVEVNILRGTIHKVGGSRSGELVVTFQGDDPAIKAAKSWLTAQ
                     GIVVESTVVGETDHA"
     gene            173..826
                     /locus_tag="PLW80_11065"
     CDS             173..826
                     /locus_tag="PLW80_11065"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HPB67098.1"
                     /translation="MLELLDLLIRPTLETVAMVAASTLAAVLLGAPVGLFLYLIAPGG
                     LSERPALRRVLDRAINALRSFPFIILMIMLFPLSRILIGTSIGTAAAIVPLAIAAAPF
                     VARLVESALSELDRGVVDAAVASGATKARIALAILLPEALPSLVSGLSLTVINLIGYS
                     AMAGAIGGGGLGDLAVRFGYHRFRLDVMTAAVLVILVLVEGAQAAGNAAVRRIKSMR"
BASE COUNT          170 a          396 c          338 g          198 t
ORIGIN      
        1 ttccccgtcg aggtcaatat cctccgcggc accatacaca aggtaggcgg atcgcgctcg
       61 ggcgagctcg tcgtcacgtt ccagggcgac gaccctgcga tcaaggccgc caagagctgg
      121 ttaacggctc aaggcatagt cgtcgaatcg acggtcgtcg gggagaccga ccatgcttga
      181 actgctcgac ctgctcatac gaccgacgct ggagaccgta gcgatggtcg ccgcgtcgac
      241 gctggccgcc gtcctcctag gggctcccgt cggactcttc ctgtacctga tagctccggg
      301 cggcctgtcg gagcggcccg cgcttcgccg cgtactggac cgggccatca acgcgctgcg
      361 ctcgttcccg ttcatcatac tcatgatcat gctgttcccg ctttccagga tcctgatcgg
      421 caccagcata ggcacggccg ccgcgatcgt gccgttggcc atagccgccg cgcccttcgt
      481 cgctcggctc gtcgaatcgg cgctgtccga gctagaccgg ggcgtcgtcg acgcggccgt
      541 cgcctcgggc gcgaccaagg cgcgcatcgc cttggccata ctcctgcccg aggccctgcc
      601 atcactggta tcgggcctgt cgctgaccgt gatcaatctg atcggctact cggccatggc
      661 cggggccata ggcggcggcg gactcggcga cctcgccgta cggttcggct accatcgctt
      721 ccgactggac gtgatgacgg ccgccgtgct cgtcattctc gtcctcgtcg aaggcgccca
      781 ggccgcgggc aacgcggccg tccgcaggat caagtccatg cgctagccgg cggagagtcg
      841 gctgaagcgc cgccccgtcc gtcggctcca ttaactcccc acagtccctt ttcggcgctc
      901 gtagagcgac cgcgactcat cgcaaggagg ttcctatgaa ccgcaaggcc ctggcactat
      961 cagtactgtc gctcgtagcg acgctaagct tcgctcaagc cccgcagccg gcgctcaagg
     1021 tcggagcgac gcccgttccg cacgccgaac tgctccggct cgtcgcatcg gagctcgccg
     1081 cccagggcat caaactggag gt
//
LOCUS       DAOOLN010000948         1100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_180390_length_1100_cov_3.979904, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000948 DAOOLN010000000
VERSION     DAOOLN010000948.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180390_length_1100_cov_3.979904"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..311)
                     /locus_tag="PLW80_11070"
     CDS             complement(24..311)
                     /locus_tag="PLW80_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67099.1"
                     /translation="MRCRLCGAEAAEFVSGGAGYGECPVCGYIGLEPSRLPSPTAEEA
                     RYRLHRNSRGDSGYVAWILEFIDAVEPYLQTGADLLDFGSGPEPVPAALAR"
     gene            488..>1100
                     /locus_tag="PLW80_11075"
     CDS             488..>1100
                     /locus_tag="PLW80_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB67100.1"
                     /translation="MSLIRVRSLSYTYPGAESPALSDVSLELDDGEYLAIVGANGSGK
                     STLVRCLNGLLVPPAGSVSVAGADPSDPVGRAAARRALSIVFQSPPDQLVASVVEEDV
                     AFGLENLGVPRQEMRERVAAALRDVGLESERTRSPRFLSAGQQQRLAVAGAIAMRPRA
                     VAFDEATSMIDPVGRDDVLRLMDELVAEGVAVLHVTHDMDEAAR"
BASE COUNT          163 a          360 c          397 g          180 t
ORIGIN      
        1 ctccggttgg gtgatgttca agattatcgg gcaagggcgg ccggcacggg ctcgggcccc
       61 gagccgaagt cgagcaggtc ggcgccggtc tgaaggtacg gctcgacggc gtcgatgaac
      121 tcgagtatcc acgcgacgta gcccgagtcg ccccgagagt tgcggtgtag cctatagcgg
      181 gcttcctccg cagtgggcga aggcaggcgg gacggctcga ggccgatgta gccgcagacc
      241 ggacactcgc cgtagccggc tccgcccgat acgaattccg cggcctcggc gccgcataat
      301 ctgcatctca tggcgtggat actatgacgg acggaggcgc agatggaagg ccgcgccggg
      361 cgacggcgcg gcttgacatg gaggcgagtt cttcgtagct ttggcctacg cttggatccg
      421 gtgaacggac cgagacgcga ggacgtacca tgacccacgc cacgctcgcg cgcctccgaa
      481 ggcgcgtatg tcgctgatac gtgttcgatc cctgagctat acctatccgg gggccgagtc
      541 cccggccctg tccgatgtga gcctcgagct cgacgatggc gaatatctgg ccatcgtcgg
      601 ggccaacggc tccggtaaga gcaccctcgt ccgttgcctg aacggcctcc tcgtcccgcc
      661 ggccggttcg gtctcggtgg ccggggccga tccttccgac ccggtcggca gggcggctgc
      721 ccgcagggcc ttgtccatcg tcttccagtc gcctccggac cagctcgtcg cctcggtcgt
      781 cgaagaggat gtcgcgttcg ggctcgagaa cctcggggtg ccccgccagg agatgcgcga
      841 acgcgtggcg gcggcgctgc gcgacgtcgg gctcgagtcg gagcggacgc gctcgccccg
      901 cttcctgtcg gctggccagc agcagcgcct ggccgtagcc ggagcgatcg cgatgcggcc
      961 gagggctgtg gccttcgacg aggcgacctc gatgatagac ccggtgggcc gcgacgacgt
     1021 gcttcgcctg atggacgagc tggtcgccga gggcgtcgcc gtgctccacg tgacgcatga
     1081 catggacgag gcggcccggg
//
LOCUS       DAOOLN010000949         1100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_180497_length_1100_cov_2.307177, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000949 DAOOLN010000000
VERSION     DAOOLN010000949.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180497_length_1100_cov_2.307177"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..>1100
                     /locus_tag="PLW80_11080"
     CDS             60..>1100
                     /locus_tag="PLW80_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB67101.1"
                     /translation="MDLEKRYSRLPDRDYPNFRAMFDSIFDRFGGLPAVKAKPEPGSP
                     YTVWSYADVRRESYAVGRFLLSKGLSAGDRVCVMAENRPEWCFAYIGIVAAGLVVVPV
                     DSTMDAAGLAGIIAASRSRALFYSPALGAKVAEALVQSPVDLAIEFPAVVGERKPAEK
                     PNAAASGAKERYGWLELGSPDDAGSGGAPEKSAERDARGLPAPAALDGDAPAVIIYTS
                     GTTGLAKGIVLSHRGVIANVNASIQSLNVDTRDVFIAVLPLHHTYAATCTFLSPMAAG
                     GDIVFIERIVPSVVLKHIREAGVTMVIGVPLLFDKIKQGIRAEVQKLRPLVRGLIGVL
                     MGVSGFCAKSLRV"
BASE COUNT          173 a          384 c          354 g          189 t
ORIGIN      
        1 gccctgcgcg gcctgcgagc tcgacctgga atcgctcctg taacggccgg ggaggtcgca
       61 tggatctaga gaaacgatac tccaggctgc ctgatcggga ctatccgaat ttcagggcga
      121 tgttcgactc gatcttcgac cggttcggcg gcttgcctgc ggtcaaggcc aaacccgaac
      181 ccggctcgcc atatacggta tggtcctacg ccgacgtccg ccgcgaatcg tacgccgtcg
      241 ggcggttcct cctgtccaag ggcctgtccg ccggcgatcg cgtctgcgtg atggcggaga
      301 accggcccga gtggtgtttc gcctatatcg gcatcgtggc cgccggtctc gtcgtcgtgc
      361 cggtggactc gacgatggac gctgccggcc tcgccggcat catcgccgcg tccagaagcc
      421 gggcgctgtt ctactcaccg gcgctcgggg cgaaggtggc cgaggcgctc gtccagtcgc
      481 cggtcgatct cgcgatcgaa ttccccgcgg tcgtcggcga gcggaagccg gccgagaagc
      541 caaacgccgc cgcgtccggg gccaaggaac gctacggctg gctcgagctt ggctcgccgg
      601 atgacgccgg aagcggcggc gcgcctgaaa aaagcgcgga gcgcgacgcc cgagggctgc
      661 cggccccggc cgccctcgat ggtgacgcac ccgcggtcat catctacacc tccggcacga
      721 caggcctggc caagggcata gtcctaagcc accgcggggt catagcgaac gtcaacgcct
      781 cgatccagtc gctcaacgtc gatacgcggg acgtcttcat cgcggtgctg cctctgcacc
      841 atacctacgc cgccacctgc accttcctgt cgccgatggc cgccggcggc gacatcgtgt
      901 tcatcgagcg catcgtccca tcggtcgtcc tcaagcatat ccgcgaggcc ggcgtgacca
      961 tggtgatcgg cgtcccgctc ctgttcgata agatcaagca gggcatacgc gccgaggtcc
     1021 agaaactgcg cccgctcgta cgcggcctca tcggcgtcct gatgggcgtc tcgggcttct
     1081 gcgccaagtc cctacgcgtt
//
LOCUS       DAOOLN010000950         1099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_180657_length_1099_cov_3.349617, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000950 DAOOLN010000000
VERSION     DAOOLN010000950.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180657_length_1099_cov_3.349617"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1099)
                     /locus_tag="PLW80_11085"
     CDS             complement(<1..>1099)
                     /locus_tag="PLW80_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67102.1"
                     /translation="GKGTGAVVSLPLGPIAGDGWRKVTMGLDPLAALAIVSGDGESLV
                     IAGASASYAAPDTAGLVELELSGLAAGTGAAVMIDELALEGARDGFSALAAASLSFGD
                     RTEKRGAYLLAEASGELADAAVVASRLEAGLNAPFAELSLSASPAYASGQASAGLGYA
                     VALPGRRSPWRVVDEYSVDEALGLYARELQAAARSGLFTASAVAGSSQEPNAFDQAWK
                     ASVGVAGVATATMAASLASSEAVVSGLGFGEAWLDSWSLLLPASEADADSRRLELAAD
                     AAGSRLTLSAYRDYERGSVPASSVVAKASLPTRFGPVSIAPYYSRKASVERASESGSF
                     AGDVADFYRAVVAAGTLWSSVPIVELWSADAF"
BASE COUNT          196 a          389 c          385 g          129 t
ORIGIN      
        1 cgaaggcgtc ggccgaccac agctcgacga tgggtacgga ggaccagaga gtcccagcgg
       61 ccaccacggc ccggtagaag tcggctacgt cgccggcgaa ggagcctgac tcgctggcgc
      121 gctccacgct cgccttgcgc gaatagtacg gagcgatcga cacggggccg aaccgcgtag
      181 gcaggctcgc cttggcgacc acggacgacg cgggaacgga accgcgctcg tagtcgcggt
      241 aagccgacag agtcaagcgc gagcccgcgg cgtcggcggc cagttccagg cgcctggaat
      301 cggcgtcggc ctcggaggcc ggtagtagga gcgaccagga atcgagccag gcttcgccga
      361 aaccgagtcc ggagacgacg gcctccgacg aggcgagcga tgccgccatg gtcgccgtcg
      421 cgacgcctgc gacgccgacg ctcgccttcc aggcctggtc aaacgcgttc ggctcctgac
      481 tggatcctgc aacggcgctc gcggtgaaca gacccgagcg agccgccgcc tggagctcgc
      541 gggcgtataa gccgagcgcc tcgtccacgg agtactcgtc gacgacgcgc catggcgaac
      601 gccggccggg tagcgcgacc gcgtagccaa ggccagccga ggcctggcca gaagcatagg
      661 ccggcgaggc gcttagggac agctcggcga aaggcgcgtt caggcctgcc tcgagcctgg
      721 aagccaccac ggcggcatcg gccagttcac cggacgcctc ggctagcagg tacgccccgc
      781 gcttctccgt gcggtcgccg aacgataggc tcgcggccgc caacgccgag aaaccgtcgc
      841 gggcgccctc gagcgcaagc tcgtcgatca tgaccgcggc gccggtcccg gccgccaagc
      901 cggatagctc gagctcgacg agtccggccg tgtccggcgc cgcgtacgag gcagaggcgc
      961 cggcaatgac gagcgactca ccgtcgccgg atacgatagc cagtgcggcc aacggatcga
     1021 gccccatcgt caccttgcgc cagccgtcgc cggctatagg gccgagcggc agactgacga
     1081 cggcgccagt tcccttacc
//
LOCUS       DAOOLN010000951         1098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_181000_length_1098_cov_2.672100, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000951 DAOOLN010000000
VERSION     DAOOLN010000951.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181000_length_1098_cov_2.672100"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PLW80_11090"
     CDS             complement(<1..472)
                     /locus_tag="PLW80_11090"
                     /inference="COORDINATES: protein motif:HMM:NF013161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPB67103.1"
                     /translation="MSVTLRGPVRDVLAFVAPFRERSRRFRDAPGEPKAPGHVTELAK
                     ALHPAGLQLRILSVRDDSPTTRTYRLAQADGSACLPPFRAGQYLSVFVEAGEVRASRP
                     YSISSTPDEAEGGGYYELTVKNAPGGFCAPLILGTWEPGTTVRCSAPAGVFYHEP"
     gene            complement(469..>1098)
                     /locus_tag="PLW80_11095"
     CDS             complement(469..>1098)
                     /locus_tag="PLW80_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67104.1"
                     /translation="AVNARKHGFSVVVVYLGLDADREALGLADYSYFIAPHMDTRLLY
                     DSLYDLDSDQVMQASICLNSAIPDCSPPGTAIVSITAGIRPEAWSCVEAKDYFRIKSL
                     LADKLIGQFEAATGARLRGHIEELEVATPVTFARYTGAWDGVVYGYEPAPWDSILPRA
                     ISLDRERYLAGLDFCGGFSYRCHGYGSSILSGKAAAERTLARLGARGGR"
BASE COUNT          168 a          362 c          409 g          159 t
ORIGIN      
        1 gcggctcatg gtagaagacg ccggccggag cggagcagcg gacggtcgtg cccggctccc
       61 aggtcccgag gatgagcggc gcgcagaagc cgcctggcgc gttcttgacc gtcagctcgt
      121 agtagcctcc gccttccgcc tcgtccgggg tcgacgatat ggagtagggc cgcgaggcgc
      181 gtacctcgcc cgcctcgacg aatacgctca ggtactggcc ggccctgaac ggcggtaagc
      241 acgccgaacc gtcagcctgg gccaggcggt aggtcctcgt cgtcgggctg tcgtcgcgta
      301 cggacagtat ccgcaactgg agcccggccg gatgcagggc cttggccagc tccgtcacgt
      361 gtccgggagc cttgggctcg ccgggcgcgt cgcggaagcg ccgcgagcgc tcgcggaacg
      421 gcgcgacgaa ggccaggacg tcgcgaacgg ggccgcgtag cgtcacgctc atcgtccgcc
      481 ccttgcgccg agcctggcca gggtccgctc ggcggcggcc ttgcccgaga ggatggagct
      541 accgtagccg tggcagcggt acgagaagcc gccgcagaag tccaggccgg ccaggtagcg
      601 ctctcggtcc agggatatgg cgcgcggcag gatggagtcc cagggcgccg gctcgtagcc
      661 gtagacgacg ccgtcccagg cgccggtgta gcgcgcgaag gtgaccggcg tcgcgacctc
      721 gagctcctcg atatggccgc gcagccgcgc gccggtggcc gcttcgaact ggcctatcag
      781 cttgtcggcc aggagcgact tgatcctgaa gtagtccttg gcctcgacgc aggaccaagc
      841 ctcgggcctg atgccggcgg tgatggacac gatggcggtg cccggcggcg agcagtcggg
      901 gatggccgag ttgaggcaga tggacgcctg catcacctgg tccgagtcca ggtcgtacag
      961 cgagtcgtac aggagccgcg tgtccatgtg cggcgcgatg aagtagctgt agtcggccag
     1021 gccgagcgcc tcccggtcgg cgtccaggcc caggtagacg acgacgaccg agaagccgtg
     1081 cttcctggcg ttcacggc
//
LOCUS       DAOOLN010000952         1098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_181011_length_1098_cov_2.587728, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000952 DAOOLN010000000
VERSION     DAOOLN010000952.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181011_length_1098_cov_2.587728"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1098)
                     /locus_tag="PLW80_11100"
     CDS             complement(<1..>1098)
                     /locus_tag="PLW80_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010698174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPB67105.1"
                     /translation="SQASRRIKELCGNPEAAPSAYSLFDYEIAWELGGWDALGSLRER
                     ALARGIRLAADMVPNHTGLDSAWVRDRPDLFVRRPNPPFPSYSYTGENLSGDGAIGVW
                     LEDHYYDRRDAAVTFKRVDFRSGETSYVYHGNDGTSMPWNDTAQIDFLNPAAREAVRE
                     RILHVARNFPIIRFDAAMVLAKRSLRRLWYPEPGSGGAIASRFESAMSAEDFNAAIPE
                     EFWREVVDQCAAEAPDTLLLAEAFWMMEGYFVRTLGMHRVYNSAFMNMLKDKKNAEYR
                     ATIRNTVEFDKDILKRFVNFMNNPDEETAIAQFGDGDRYFGVCAMMATMPGLPMFGHG
                     QIEGFGEKYGMEYRRAYRDESPNEALVRRHER"
BASE COUNT          160 a          358 c          382 g          198 t
ORIGIN      
        1 cgttcgtggc ggcggaccag ggcctcgttc gggctctcgt cgcggtaggc ccgccggtac
       61 tccatgccgt acttctcgcc gaagccctcg atctggccgt ggccgaacat cggcaggccg
      121 ggcatcgtgg ccatcatcgc gcagacgccg aagtagcggt ctccgtcgcc gaactgagcg
      181 atggccgtct cctcgtcggg gttgttcatg aagttgacga agcgcttgag gatgtccttg
      241 tcgaactcga cggtgttgcg tatcgtcgcg cggtactccg cgttcttctt gtccttgagc
      301 atgttcatga aggccgagtt gtacacgcgg tgcatgccca gggtccgtac gaagtagccc
      361 tccatcatcc agaaggcctc ggccaagagg agggtgtcgg gcgcctcggc cgcgcactgg
      421 tcgaccacct cgcgccagaa ttcctccggt atcgccgcgt tgaagtcctc ggccgacatg
      481 gccgactcga agcggctcgc gatcgcgccg ccggagcccg gttcggggta ccagaggcgg
      541 cgcagcgagc gcttggccag caccatcgcc gcgtcgaagc gtatgatggg gaagttcctg
      601 gccacgtgca ggatgcgctc cctcaccgcc tcgcgggcgg ccgggttcag gaagtctatc
      661 tgggcggtgt cgttccaggg catgctcgtg ccatcgttgc cgtggtagac gtagctcgtc
      721 tcgccggagc ggaagtcgac gcgcttgaag gtgacggccg cgtctcgccg gtcgtagtag
      781 tggtcctcga gccagacgcc gatcgcgccg tcgccggaca ggttctcgcc cgtgtacgag
      841 tacgacggga agggcgggtt aggccgacgc acgaataggt cgggccggtc gcgcacccag
      901 gcggagtcca ggccggtatg gttcggcacc atgtcggcgg cgagccgtat gccccgcgcc
      961 agcgcgcgtt cccggagcga gccgagcgcg tcccagccgc cgagttccca ggctatctcg
     1021 tagtcgaaca gcgaataggc gctcggcgcc gcctccgggt tgccgcacag ctccttgatg
     1081 cggcgcgagg cctggctg
//
LOCUS       DAOOLN010000953         1098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_181062_length_1098_cov_2.104506, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000953 DAOOLN010000000
VERSION     DAOOLN010000953.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181062_length_1098_cov_2.104506"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PLW80_11105"
     CDS             complement(<1..424)
                     /locus_tag="PLW80_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67106.1"
                     /translation="MPTDETDAGRLGPLLNVAMACAALGAAALTLVPVLNLILARLFW
                     RGLPEAGPLTEHALIALAFFAAAVAGIRGTHLSLGSAGAAKGRLGMTIAGLRSAFSTA
                     VDTTLFFAGLSLVLIGFEPADRAFGVPIAVFASPLAVGY"
     gene            complement(426..>1098)
                     /gene="dctP"
                     /locus_tag="PLW80_11110"
     CDS             complement(426..>1098)
                     /gene="dctP"
                     /locus_tag="PLW80_11110"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HPB67107.1"
                     /translation="SVIRDDAEFDAAIAAVLPILRKRLGDRYEIVAVAKAGWIRFFSN
                     RPLRSPEDLRNLRMGVNRNMDALTKVMQSLGVRTVQADSASTLLQFNSGAMDAMYSSP
                     LFVGALWSQYRRVVTHMTGFKVAPFFGAIIVNRRSWDRVPEALKPALREAAESICRDI
                     GNEAARLEDEGIAAMTRGGLQVPPYAQADAAAWNELFSSTVEGFMADWYSREFTAAVY
                     EAIGR"
BASE COUNT          189 a          360 c          384 g          165 t
ORIGIN      
        1 gatatcccac ggccagcggc gaagcgaaga cggcgatcgg gacgccgaag gcccgatcgg
       61 ccggctcgaa gccgatcagt acgagcgaca ggccggcgaa gaacagcgtc gtatcgacgg
      121 ccgtggagaa ggccgacctg agcccggcta tcgtcatccc gagtcggccc ttcgcggcgc
      181 cggcggagcc gagcgatagg tgtgtccccc ggataccggc gacggcagcc gcgaagaacg
      241 cgagcgctat gagcgcgtgc tcggtcagcg gacccgcttc gggcaggcct cgccagaaga
      301 ggcgggccag gatgaggttc aatacgggga ccagcgtcag cgcggccgcg cctaaggcgg
      361 cgcaggccat agccacgttc aggagcgggc cgagacgccc ggcgtccgtt tcgtccgtcg
      421 gcatgctaac ggcctatggc ctcgtagacg gcggcggtga actcccggct gtaccaatcc
      481 gccatgaagc cttccacggt cgacgagaac agctcgttcc aggcggccgc gtccgcctgc
      541 gcgtagggcg gcacctgtag gccgcctcgc gtcatcgccg ctatgccctc gtcttcgagc
      601 ctggccgcct cgttccctat atcgcggcag atactctcgg cggcttcgcg cagggccggc
      661 ttgagcgctt cgggaaccct gtcccaggac ctacggttga cgatgatggc gccgaagaag
      721 ggggcgacct tgaaaccggt catgtgcgtg acgacgcgcc ggtactgcga ccagagcgcg
      781 ccgacgaaca gcgggctcga gtacatcgcg tccatggcgc cggagttgaa ttgcagtagc
      841 gtcgaggccg agtccgcctg cacggtgcgc acgcccaggg attgcatcac cttggtcagc
      901 gcgtccatgt tacggttgac gcccatgcgc aggttgcgca ggtcctcggg cgagcgtagc
      961 ggccggttcg agaagaagcg tatccagccg gccttggcga ccgccacgat ctcgtagcga
     1021 tcgccaaggc gcttcctcag gatgggtagg acggcggcga tcgccgcgtc gaattccgcg
     1081 tcgtcgcgta tgaccgag
//
LOCUS       DAOOLN010000954         1098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_181072_length_1098_cov_2.025887, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000954 DAOOLN010000000
VERSION     DAOOLN010000954.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181072_length_1098_cov_2.025887"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..320)
                     /locus_tag="PLW80_11115"
     CDS             complement(51..320)
                     /locus_tag="PLW80_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPB67108.1"
                     /translation="MKATLVIVRGQVQGIGFRYETRMVARSLGLVGWVSNISDGGVET
                     YAEGTEVAIGKFLQWLQHGPPGARVTSIDVTERTAGGKYTSFMIE"
     gene            complement(325..684)
                     /locus_tag="PLW80_11120"
     CDS             complement(325..684)
                     /locus_tag="PLW80_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB67109.1"
                     /translation="MSDTEDGTILIVEDEDAIRLTLRDFLRKKGFDVLVASDGVGAIK
                     ILLDSNVDLVLTDYRMDLLGGDYWIKFLKKYCPELKVIITSGFLRPDFEIPYEVIYKP
                     FDYSALEKRVREILCNR"
     gene            complement(687..>1098)
                     /locus_tag="PLW80_11125"
     CDS             complement(687..>1098)
                     /locus_tag="PLW80_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB67110.1"
                     /translation="ELSAANNPELDWNCLAIIDDDEQLNEIIKKFLEKEGFSFNIVQA
                     YDGFAAGALLTERKPGFIILDIDLPGLDGNAICRKIKSDPAFGKPFVITVTGLDAQSI
                     KTMALDAGADAFLSKPLDFDALRTIMIDFSGKVR"
BASE COUNT          243 a          293 c          287 g          275 t
ORIGIN      
        1 tacccgagct tcccaagctc gtgaagcggg ttcgactccc gtcggtcgct ctattcgatc
       61 atgaagctgg tatactttcc gccagcggtt ctttccgtaa catcgataga cgttacccgc
      121 gctcccggcg gcccatgctg tagccattgt aaaaatttac ctatagcgac ctctgtcccc
      181 tcggcgtagg tttctacgcc gccgtccgat atattggaga cccagccgac gagaccaagg
      241 gaacgcgcga ccatacgcgt ttcgtagcgg aagccgatgc cctgtacttg gcctcgaacg
      301 atgacgagcg tcgctttcat cgagtcatcg gttacagagg atctcacgga ctcgcttctc
      361 tagggccgag taatcgaagg gtttgtatat gacctcgtac ggtatttcga agtcggggcg
      421 taggaagccc gaggttatga taaccttcaa ctctggacag tatttcttta gaaacttaat
      481 ccaataatcg ccacccaata aatccatgcg gtaatcggta agcacaaggt cgacgttcga
      541 atcgagcagt attttaatcg cgcccacgcc gtcggacgcg acgaggacat cgaagccctt
      601 ttttcgaagg aagtcccgca aggtcagcct gatagcgtct tcgtcctcga cgatcaagat
      661 ggtcccgtct tctgtgtcgc tcatcgttac cgaacctttc ctgaaaaatc gatcatgatc
      721 gtcctgaggg cgtcaaaatc gagcggctta gaaagaaatg cgtcggctcc ggcatcaagg
      781 gccatggttt ttatgctttg ggcgtccagg cctgttacgg taataacgaa gggttttccg
      841 aacgcggggt cggatttgat tttgcggcaa atagcgttac catcgaggcc tggtagatcg
      901 atatcgagga tgatgaaccc cggttttcgc tccgtgagca aggctccagc ggcgaatcca
      961 tcatacgcct ggacgatgtt gaaggagaac ccttcctttt caaggaattt cttgatgatc
     1021 tcgttgagct gctcgtcatc gtctatgatc gcgaggcaat tccagtccag ttcgggattg
     1081 tttgccgcgg atagctcc
//
LOCUS       DAOOLN010000955         1095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_181707_length_1095_cov_3.307692, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000955 DAOOLN010000000
VERSION     DAOOLN010000955.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181707_length_1095_cov_3.307692"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /gene="cysS"
                     /locus_tag="PLW80_11130"
     CDS             complement(<1..1006)
                     /gene="cysS"
                     /locus_tag="PLW80_11130"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPB67111.1"
                     /translation="MGLRLYNTLGRSLQEFEPIQPGKVGFYGCGPTVYNYAHIGNMRM
                     YVFEDTLARALRYLGYEVTHVMNITDVGHLSGDADSGEDKMVKSAQERHKSVLEIARY
                     YTDAFFADEAKLNILRPDVVCNATEHVDDMISLIRRLEANGYTYFAGGNLYFDVSKFP
                     SYGEMAGLKLDELKAGARIEVDANKRNPYDFVLWFTKSKFEDQALQWDSPWGRGYPGW
                     HVECSAMSMKYLGEQFDIHAGGIDHVAIHHTNEIAQSEGATGKKWVNYWLHGEFLVLD
                     KGKMSKSAGGFLTLKTLTDAGYEALDYRYFLLGAHYRSQLQFSYDGLDSARASRRSLV
                     E"
BASE COUNT          206 a          321 c          347 g          221 t
ORIGIN      
        1 gctcgaccag ggagcgccgc gaagcccggg cggaatccaa accgtcgtag gagaactgaa
       61 gctgggaccg gtagtgggcg cccaggagga agtagcggta gtcgagagcc tcgtagcccg
      121 cgtcggtcag ggtcttgagc gtgaggaatc cgcccgccga cttggacatc ttgcccttgt
      181 ccaagacgag gaactcgccg tggagccagt agttgaccca cttcttgccg gtggctccct
      241 cgctctgggc tatctcgttc gtgtggtgga tggccacgtg gtctatgccg ccggcgtgta
      301 tgtcgaactg ctcccctaag tacttcatgc tcatcgcgga acactcgacg tgccagccgg
      361 ggtagccgcg cccccagggg ctatcccact gcagcgcctg gtcctcgaac ttggacttcg
      421 tgaaccagag gacgaagtcg tagggattgc gcttgttggc gtcgacctct atgcgcgctc
      481 ccgccttgag ctcgtcgagc ttgaggccgg ccatctcgcc gtagctcggg aacttggaca
      541 cgtcgaagta gaggttgccg ccggcgaaat aggtgtagcc gttggcctcg agccgccgta
      601 tcaggcttat catgtcgtcc acgtgctcgg tggcgttgca gacgacgtcg ggtctgagga
      661 tattgagctt ggcctcgtcg gcgaagaagg cgtcggtata ataccgcgct atctcgagaa
      721 cggacttgtg ccgctcctgg gcgctcttga ccatcttatc ctcgcccgag tccgcgtcgc
      781 cggacaggtg gccgacgtcg gtgatgttca tcacgtgcgt aacctcgtag ccgaggtagc
      841 ggagcgcgcg ggcgagcgta tcctcgaaga cgtacatgcg catgttgccg atgtgcgcat
      901 agttatagac ggtcgggccg catccgtaga agcctacctt ccccggctgt atcggctcga
      961 actcctgcag ggaacgtccg agcgtgttgt agagtcgcaa acccatgcgg tcctcctttc
     1021 aagatatgcg tcgaacagaa ctcatacaat aaaacggcat ttgaaccacg gagacacaga
     1081 gacacggaga ggaca
//
LOCUS       DAOOLN010000956         1092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_182507_length_1092_cov_3.443587, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000956 DAOOLN010000000
VERSION     DAOOLN010000956.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182507_length_1092_cov_3.443587"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PLW80_11135"
     CDS             complement(<1..424)
                     /locus_tag="PLW80_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67112.1"
                     /translation="MEDRARDEIRDDILERVFPELRFGEDPDIERYFELRASGRMLDA
                     LAVYRSRLRPRYPDDAQRVVLLKLYRIKSPAFPEYLRGLMRARADDIIARLRANIDAL
                     LEPLSGIKLGKTYAVLKAVERMARMLPDDPERARQAAWA"
     gene            complement(536..>1092)
                     /locus_tag="PLW80_11140"
     CDS             complement(536..>1092)
                     /locus_tag="PLW80_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024752187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HPB67113.1"
                     /translation="TSEDRMQIQVEVSQLVDEIDRIASHAQFNGMNVLTGRFARESGE
                     NIVTGSMWFHIGANMDQRERVYIGTMTAKGLGVKNVGDDSILKLSDPDSSNRSIGVLD
                     SALKTVNKQRADLGAYQNRLEHAVRGIDIGAENLQAAESRIRDADMASEMVEFTKNQI
                     LAQAGNAMLAQANQKTQQVLQLLQ"
BASE COUNT          160 a          330 c          378 g          224 t
ORIGIN      
        1 aggcccaggc cgcctgcctg gcccgctccg ggtcgtcggg tagcatcctg gccatgcgct
       61 cgacggcctt gagcaccgcg taggttttgc ccagcttgat gccggacagg ggctcgagta
      121 gcgcgtcgat gttggcgcgt aggcgggcga tgatatcgtc ggcccgcgcg cgcatgaggc
      181 cgcgtagata ctcggggaag gccggggact tgatcctgta tagcttgagg aggacgacgc
      241 gctgggcgtc gtccgggtag cgggggcgga ggcggcttcg gtagacggcg agcgcgtcaa
      301 gcatgcgccc cgacgctcgt agctcgaagt agcgctctat gtccggatcc tcgccgaagc
      361 gtaattctgg gaagacgcgc tcgagtatat cgtcgcgaat ctcgtcccgg gctctatctt
      421 ccatctggcg gcattatacg cggctctcgg acgctcttca agcgagcggt gctggtccga
      481 aaaaaaaggg ccgcccccgg aagggcggcc ctcgtcacgt ccgcgcggcc acggcctact
      541 ggaggagctg cagtacctgc tgggtcttct ggttggcctg ggcgagcatc gcgttgccgg
      601 cctgcgcgag gatctggttc ttggtgaact cgaccatctc gctggccatg tccgcgtcgc
      661 ggatgcggct ctcggcggcc tggaggttct ccgcgccgat gtcgatgccg cgtaccgcgt
      721 gctcgagcct gttctggtag gctcccaggt ctgcgcgctg cttgttgacg gttttcaggg
      781 cggaatcgag taccccgatg gacctgttgg agctgtccgg atcggacagc ttcaggatgg
      841 aatcgtcgcc gacgttcttg acgccgaggc ccttggcggt catcgtgcct atgtagacgc
      901 gctcccgctg atccatgttg gcgccgatgt ggaaccacat cgacccggtg acgatgttct
      961 ctcccgattc gcgggcgaaa cggccggtaa gcacgttcat gccgttgaac tgggcgtggc
     1021 tggcgatccg gtcgatctcg tcgacgagct gagagacctc tacctggatc tgcatgcggt
     1081 cctccgaggt at
//
LOCUS       DAOOLN010000957         1092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_182598_length_1092_cov_2.365477, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000957 DAOOLN010000000
VERSION     DAOOLN010000957.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182598_length_1092_cov_2.365477"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..>1092)
                     /locus_tag="PLW80_11145"
     CDS             complement(126..>1092)
                     /locus_tag="PLW80_11145"
                     /inference="COORDINATES: protein motif:HMM:NF018938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1446 domain-containing protein"
                     /protein_id="HPB67114.1"
                     /translation="AAVYGDDVFPRIDEYRGAAVWETGEPLERLAGEIVSANAYLGCE
                     GIVEALKRGADVVITGRVSDPALFIAPLVYEFGWKPDDWDRLGKGTVIGHLLECGGQA
                     TGGYFADPGKKDVPEPWRLGFPLIEADENGDAFITKVVGSGGVVTTDTCKEQLIYEIH
                     DPSTYITPDVTADFSRVSFTQSGKDVVEIRGATGRARTDTLKVSIGYKDCFIGEGEIS
                     YGGPNCVERARLAREIVRRRLELRAVPTDELRMDIVGVDSLYRNDAAPLPSSPSEARL
                     RVAARTKTREAAAAIANEVETLYTNGPAAAAPRQPCRKSYRSRRY"
BASE COUNT          160 a          373 c          351 g          208 t
ORIGIN      
        1 cataggctat gagcgacacg ttcgaaatat tgcccttatc gccggtcctc gagtgggcca
       61 gttcccataa tcgcatgatc acacctcctc tacggccacg acggcctcgg cgtcggccct
      121 gggtatcagt atcgacgcga ccgatatgat ttcctgcacg gttgccgtgg cgccgccgcc
      181 gccggcccgt tggtatagag cgtctcgacc tcgttggcga tcgcggccgc cgcctcgcgg
      241 gtcttggtcc tcgcggctac gcgcaggcgc gcctcgctcg gcgagctcgg caggggggcg
      301 gcgtcgttcc ggtacagcga gtcgacgccg actatgtcca tgcgcagctc gtcagtcggc
      361 acggcgcgca actcgagccg cctgcgcacg atctctcgcg ccagcctggc ccgctcgacg
      421 cagttggggc cgccgtagct gatctccccc tcgccgatga agcagtcctt gtagccgatg
      481 cttaccttga gcgtgtcggt cctggccctg cccgtggcgc cccgtatctc cacgacgtcc
      541 ttgccggact gggtgaacga taccctcgag aagtcggccg tgacgtcggg cgtgatatac
      601 gtagacgggt cgtgtatctc gtagatcagc tgttccttgc aggtgtccgt agtgacgacg
      661 ccgcccgagc cgactacctt ggtaatgaac gcgtcgccgt tctcgtccgc ctcgatgagc
      721 gggaagccga ggcgccaggg ctccggtacg tccttcttgc cggggtcggc gaaatagccg
      781 ccggtggctt ggccgccgca ttcgagcagg tggccgatga cggtaccctt gccgagccta
      841 tcccagtcgt cgggcttcca gccgaattca tagacgagcg gcgcgatgaa cagggcgggg
      901 tcggaaacgc ggcctgttat caccacgtcc gcgccccgct tgagcgcctc gacgatgccc
      961 tcgcatccta ggtaagcgtt ggccgatacg atctcgcctg cgagccgctc gagcggctcg
     1021 ccggtctccc agacggcggc gccgcggtac tcgtcgatcc tgggaaacac gtcgtcgccg
     1081 tagacggcgg ct
//
LOCUS       DAOOLN010000958         1092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_182614_length_1092_cov_2.268081, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000958 DAOOLN010000000
VERSION     DAOOLN010000958.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182614_length_1092_cov_2.268081"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PLW80_11150"
     CDS             <1..344
                     /locus_tag="PLW80_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB67115.1"
                     /translation="SFLARARAALRPGGRLYLITPNADGLQARLMGSRWRSAIRDHLY
                     LFSARTLGAMLADEGYSVEYIGTWGGWPVGMRPVSLKRPMDRLAKRFGLGDVMVVRAS
                     QAAVPTPLEVR"
     gene            341..1054
                     /locus_tag="PLW80_11155"
     CDS             341..1054
                     /locus_tag="PLW80_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPB67116.1"
                     /translation="MTDDYRITVAQAVENVLERMGAACARAGRSADEVRLLAVTKFHP
                     YEAVEAAYAAGVRLFGENRVQEAEAKFSAGTAPKGAFVHLLGHLQSNKAKRAAALFDC
                     VQSIDSIEVAAELAKRAAALGKTLDVLLELHTGEESKTGFASEDALLRALEAIMPMPS
                     LKPRGLMTMAPFSDDAAVVRASFRTCRRAFEVAMKRFAPVDFSVLSMGMSNDFELAVE
                     EGSTMVRVGTAIFGSRRLR"
BASE COUNT          182 a          354 c          366 g          190 t
ORIGIN      
        1 gctccttctt ggccagggcg agggccgccc tgcgccccgg aggccgactg taccttataa
       61 cgccgaacgc cgacggtttg caggccaggc tgatgggctc tcgatggcgc agcgccattc
      121 gcgatcacct atacttgttc tcggcgagga cgcttggcgc gatgctggcg gacgagggct
      181 attccgtcga gtacatcggc acctggggcg gttggcctgt cggcatgcgc cccgtcagcc
      241 tgaaacggcc catggatagg ctggccaagc ggttcggctt gggcgacgtc atggtcgtca
      301 gggcttctca ggccgcggtt ccgacgcctc tggaggtacg atgaccgacg actacaggat
      361 aaccgtcgcc caggcggtgg agaacgtgct cgagaggatg ggagcggcct gcgccagggc
      421 cggacggtcg gccgacgagg tacgcctact cgccgtcaca aaattccatc cctacgaggc
      481 ggtggaagcg gcctacgccg ccggtgtcag gctattcggc gagaaccgcg ttcaggaagc
      541 cgaggccaaa ttttccgccg gcacggctcc gaagggcgcc ttcgtccacc tgctcggcca
      601 cctccagagt aacaaggcca agcgcgccgc ggccctgttc gactgcgttc agtcgataga
      661 ctcgatagag gtcgccgccg agcttgccaa gcgcgccgcg gcgctcggca agaccctcga
      721 cgtgctcctt gaactgcata ccggcgagga atccaagacc ggcttcgcga gcgaagacgc
      781 gctcctacgc gccctggagg ccatcatgcc gatgccgtcg ctcaagcctc gcggactcat
      841 gaccatggcg cccttcagcg atgacgccgc ggtcgtacga gcgtcgttca ggacctgccg
      901 tagggctttc gaggtggcca tgaaacggtt cgccccggtc gatttctccg tgctgtccat
      961 gggcatgtcg aacgatttcg agctggccgt ggaggaaggc tcgacgatgg tgcgcgtcgg
     1021 cacggccatc ttcggaagca ggcggcttcg atgaggctcg ccaggagcgt cgcggcgcta
     1081 tcgctcgcgc tc
//
LOCUS       DAOOLN010000959         1092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_182617_length_1092_cov_2.247830, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000959 DAOOLN010000000
VERSION     DAOOLN010000959.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182617_length_1092_cov_2.247830"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PLW80_11160"
     CDS             <1..609
                     /locus_tag="PLW80_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB67117.1"
                     /translation="RDTIVKIGEGGEVLIKGPQVMKGYYKNEAATKEVFTDDGFFRTG
                     DIGEIDEQGRLKITGRIKDLIITSGGKNISPQNLENSIKASSFIEQVAVIGDNRKYLS
                     ALIVPAWAELEGWAKANNIAFSSRADLIANTEVNRLFEQEIERCTQDYARVEQIRKFR
                     LLEKEWSQETDELTPTLKLKRRVISQKYAKEIETMYPPDDGL"
     gene            863..>1092
                     /locus_tag="PLW80_11165"
     CDS             863..>1092
                     /locus_tag="PLW80_11165"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB67118.1"
                     /translation="MPAIFSNQADNLKDAACVAYKNAQGQYTDISWNSMAEMVRNIGY
                     FLLSKGIKVQDKVALFSDNRYEWWVAELGILSA"
BASE COUNT          268 a          322 c          315 g          187 t
ORIGIN      
        1 agggacacga tcgtcaagat cggcgaagga ggcgaggtgc tcatcaaagg cccgcaggtg
       61 atgaagggct actacaagaa cgaggccgcc accaaggagg tcttcaccga cgacggcttc
      121 ttcaggaccg gcgatatcgg cgagatcgac gagcagggcc ggctcaagat caccggccgc
      181 atcaaggacc tgatcatcac ctcgggcggc aagaacatct cgccccagaa cctcgagaac
      241 agcatcaagg cctccagctt catcgagcag gtggccgtca tcggcgacaa ccgcaagtac
      301 ctctcggccc tgatcgtacc ggcctgggcc gagctggagg gctgggccaa ggccaacaac
      361 atcgcctttt ccagccgcgc cgatctcatc gcgaataccg aggtgaacag gctctttgaa
      421 caggagatcg agcgctgcac ccaggactac gcgcgcgtgg agcagatccg caagttcagg
      481 ctcctggaga aggaatggtc gcaggagacc gacgaactga cgcctacgct caagctcaag
      541 cgccgcgtta tcagccagaa gtacgccaag gagatcgaga ccatgtatcc gcccgacgac
      601 ggcctctagc gccgagcctg ccatgcgagg gggtggagga tgatgcatcg ccctccaccc
      661 ccttgttctg ctcacaccga accgggacca cggccctgac ccggggggcc gagcaaccca
      721 tgtcctccga tactgcattg atactccatc gatatttgtt ggcacatccg gttaactagt
      781 ttataataac gcgtgctcaa aatatcatgg taatagcaaa gaaggaggag ggtatcgaat
      841 ggctacgtat caggaaacat caatgccggc aattttctcc aaccaggccg ataatctcaa
      901 ggacgctgcc tgcgtggcgt acaagaacgc gcaagggcag tatacagaca tctcctggaa
      961 cagcatggcg gaaatggtgc gcaatatcgg ttatttcctg ctgtccaagg gcatcaaggt
     1021 ccaggacaag gtagcgttgt tctccgacaa ccgctacgag tggtgggtcg ccgagctcgg
     1081 catcttatcg gc
//
LOCUS       DAOOLN010000960         1090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_183115_length_1090_cov_2.277295, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000960 DAOOLN010000000
VERSION     DAOOLN010000960.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183115_length_1090_cov_2.277295"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PLW80_11170"
     CDS             <1..538
                     /locus_tag="PLW80_11170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPB67119.1"
                     /translation="EVVILRANNGRAVEYDEKVDAAPAKAYAGLEDSVLAMGKLKVGA
                     LAGSIDVTSSIALKQRRVMGVALPVVRTEFAAGGPYYSPLDTSASIDNAVAAFRDALA
                     MLGRSAELRVSIVRLAREVRKTIRKVNALEKIAIPELDETLHYIQNRLEESERDMFVL
                     MKMVKDRLSRPDSEVSQG"
     gene            535..1023
                     /locus_tag="PLW80_11175"
     CDS             535..1023
                     /locus_tag="PLW80_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPB67120.1"
                     /translation="MTRPIRKIMVYVDGSEQSITAAQYAIVLAKTLGADLYALYVVDT
                     RALAELVNSRIFLESEQEEYRRDLDGDASRYLNHVRELARRKGVPVAAEKASGAVHVE
                     IKAKVASLGVDLLVLGEISQIRSRRDELYNETERAMRTAQCPVLVAKGEDKIWDLYES
                     LE"
BASE COUNT          223 a          315 c          378 g          174 t
ORIGIN      
        1 cgaggtcgtc atcctgcgcg ccaacaatgg ccgggcggtc gagtacgacg agaaggtgga
       61 cgcggcgccc gcgaaggcct acgccgggct cgaggactcg gtgctcgcga tgggcaagct
      121 gaaggtcggc gcgctggccg gctcgatcga cgtgacctcg tcgatagcgc tcaagcagcg
      181 ccgcgtgatg ggcgtagcgt tgccggtcgt ccggaccgag ttcgcggcgg gggggccgta
      241 ctacagcccg ctcgatacaa gcgccagcat cgacaacgcg gtggccgcct tccgcgacgc
      301 gctcgcgatg ctcggccggt ccgccgagct gcgcgtctct atcgttaggc tggccaggga
      361 ggtgcgcaag acgatacgta aggtgaacgc gctcgagaaa atagcgatac cggaattgga
      421 cgaaacgctg cattatatac agaacaggtt ggaagagagc gagcgcgaca tgttcgtcct
      481 catgaagatg gtcaaggaca ggctatcgcg accggattca gaggtatcgc aaggatgacg
      541 aggccaatac gcaagatcat ggtctatgtg gacggctccg agcagtcgat cacggcggcc
      601 cagtacgcca tcgtgctggc caagacgctc ggcgccgacc tatatgcgct ctacgtcgtc
      661 gatacgaggg ccctcgccga gctcgtcaac tcgcggatct tcctcgagtc ggagcaggag
      721 gagtacaggc gcgacctcga tggcgacgcc tcgcgctatc tgaaccacgt gcgagaactg
      781 gccaggcgca agggcgtgcc ggtggcggcg gagaaggcct cgggcgcggt gcatgtcgag
      841 atcaaggcca aggtggcgtc cctcggcgtc gacctgctcg tgctcggcga aatatcccag
      901 atacggtcgc gtcgcgacga actctacaac gagacggagc gggccatgcg gaccgctcaa
      961 tgcccggtgc tcgtcgccaa gggcgaagac aagatatggg acctgtacga gtccttggaa
     1021 taggaaggct gacatggcgt tgatcgacct gatagaagaa agcgtagtta aggttccgct
     1081 cgcggccggg
//
LOCUS       DAOOLN010000961         1089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_183289_length_1089_cov_3.132495, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000961 DAOOLN010000000
VERSION     DAOOLN010000961.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183289_length_1089_cov_3.132495"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PLW80_11180"
     CDS             <1..240
                     /locus_tag="PLW80_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67121.1"
                     /translation="RWNTLLDPAAKANLTEDINSLVRDYLRTTLRSMRPSAFTPDRVE
                     MMAANLADRPNLLKIRNHQALEEYIRLYMVKMLKR"
     gene            353..>1089
                     /locus_tag="PLW80_11185"
     CDS             353..>1089
                     /locus_tag="PLW80_11185"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HPB67122.1"
                     /translation="MNASLLGVAFCLVFSALFSSVETAFTSLSLFQIEALKKRGRGGA
                     IVERLARKPDELISTILIGNNVVNITASALATSWALGLWGDWVLGLVTGVMTLIVLIF
                     GEVTPKRIALAHNEGVALALAPFLFVCTVLFKPFVLLVNALSSLVTRLFGDTRPDSLS
                     METMVQMMSIAEHMGVIDYAKSRMVKNVFRLGSTTVQAVMTHRIDVFSLEGSTNLEDA
                     CRLASEAAVSRVPVYVGESENIVGVVAV"
BASE COUNT          175 a          374 c          340 g          200 t
ORIGIN      
        1 cgctggaaca ccctgctcga ccccgcggcc aaggccaacc tgaccgagga tatcaactcc
       61 ctcgtgcgcg actacctgcg taccacgctc aggtcgatgc ggccgtctgc cttcacgccc
      121 gaccgcgtcg agatgatggc cgccaacctg gccgaccggc cgaacctgct caagatacgg
      181 aaccatcagg cgctcgagga gtatatccgg ctctacatgg tcaagatgct caagcgatga
      241 gggccggccc agcgtcggct caggcgcgcg ctcggcttgc gctaggcgcg gctatcgtat
      301 aggatcggct gataatggac gatccatacc catctagcgg cggaggccgc ccgtgaatgc
      361 gtcactgctg ggcgtagcct tctgccttgt cttctccgcg ctcttctcct cggtggagac
      421 ggcgttcacc tccctgtcgc tgttccagat cgaggcgctc aagaagcgcg gccgcggggg
      481 cgccatcgtc gagcgtctgg cccgcaagcc cgacgagctg atctcgacca tcctgatcgg
      541 caacaacgtg gtcaacatca ccgcctcggc cctggctacc agctgggccc tggggctctg
      601 gggcgactgg gtcctgggcc tggtcaccgg cgtgatgacc ctgatcgtgc tgatcttcgg
      661 cgaggtgacg cccaagcgca tcgccctggc gcacaacgag ggcgtcgcgc tggccctggc
      721 gcctttcctg ttcgtctgca ccgtgctgtt caagcccttc gtgctgctgg tcaacgcgct
      781 cagctcgctc gtcacgcggc tgttcggcga tacccggccg gattccctgt cgatggagac
      841 catggtgcag atgatgtcca tagccgagca catgggcgtc atagactacg ccaagagccg
      901 catggtcaag aacgtgttcc gcctcggctc cacgacggtc caggccgtga tgacccaccg
      961 catcgacgtg ttcagcctcg aaggttcgac gaacctcgag gacgcgtgcc ggctggcctc
     1021 cgaggccgcc gtctcgcgcg tgccggtcta cgtcggcgag agcgagaaca tcgtcggcgt
     1081 cgtggccgt
//
LOCUS       DAOOLN010000962         1089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_183295_length_1089_cov_2.981625, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000962 DAOOLN010000000
VERSION     DAOOLN010000962.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183295_length_1089_cov_2.981625"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..804
                     /locus_tag="PLW80_11190"
     CDS             133..804
                     /locus_tag="PLW80_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67123.1"
                     /translation="MKKICIALLVAAALFTVASCASQPAVDQPKIDEKVLTPDILEHK
                     GTALGANVPAWTMAYIESGASGVEKLAEYKGKYVIVLDAEGASKQGVLTAMDNMDAPV
                     QIARYLSTRVQQKFAGAQVGDRDQLEEYFENVVKLVSEARFSGLMAGPDWWVYLQYYK
                     PGAKSKSEVDRRIYRVIKIYSIDQKVLQDQLDKYLNDAEAAAAKTPEKQRAIDAVQSA
                     FYEGF"
BASE COUNT          223 a          333 c          331 g          202 t
ORIGIN      
        1 aaggccttta ccttcaatag cggcctttga gagcggtcac gaggggctcg tccccttgac
       61 gataggattc tgggagccgg tccctcgagg gccgaggctc ctgatcagtg aaagaccttt
      121 aaggaggtta tcgtgaagaa gatttgtatc gcgttgctgg tagccgcggc cctgttcacc
      181 gtcgcgtcct gcgccagcca gccggccgtc gatcagccca agatcgacga gaaggtcctg
      241 accccggaca tcctggagca caagggtacg gcgctcggcg ccaacgtgcc ggcctggacg
      301 atggcctata tcgagagcgg cgctagcggc gtcgagaagc tcgccgagta caagggcaag
      361 tacgtcatcg tgctcgacgc cgagggcgcg agcaagcagg gcgtcctgac cgcgatggac
      421 aacatggacg cgccggtgca gatcgcccgc tacctgtcca cgcgcgtgca gcagaagttc
      481 gccggcgccc aggtaggcga ccgcgaccag ctcgaggaat acttcgagaa cgtggtcaag
      541 ctcgtctccg aagccaggtt ctccggcctg atggccggtc ctgactggtg ggtgtacctc
      601 cagtactaca agcccggcgc caagagcaag agcgaggtcg atcgccgcat ctaccgcgtg
      661 atcaagatct actccatcga tcagaaggtg ctgcaggatc agctcgacaa gtacctcaac
      721 gacgccgagg ccgccgccgc caagaccccc gagaagcagc gggccatcga cgccgtccag
      781 agcgccttct acgaagggtt ctaaaccgga cgctcgatct gaggcatcct cggcttaggc
      841 tggggatgcc ttttttagtc ggcgccgtta acgtaccgtt tgggttatct accggaacga
      901 cctaatttca tctatactga ctaaaagtta aacaggagga ttccatgcag agaggattat
      961 atgcaagcct ggcggcgatc gcgacggttg ccctgttagc cgtttcatgc gtctcgtctc
     1021 ccaaggccgc tccggctccc gattggacgc tgacgacccc gccgccggac tcgagctata
     1081 cgtatttcg
//
LOCUS       DAOOLN010000963         1088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_183583_length_1088_cov_2.994192, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000963 DAOOLN010000000
VERSION     DAOOLN010000963.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183583_length_1088_cov_2.994192"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PLW80_11195"
     CDS             complement(<1..356)
                     /locus_tag="PLW80_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA methyltransferase"
                     /protein_id="HPB67124.1"
                     /translation="MGKQTDGGGEAFDRFFRELFGGRWDGLREALLAPGDAIDYADGL
                     REPYRLDSASVAAAHRLLLPDSGEILDACSAPGGKSLVIASRMGPSARLLANELSSDR
                     RRRLSDVLDRYLPDEA"
     gene            458..>1088
                     /locus_tag="PLW80_11200"
     CDS             458..>1088
                     /locus_tag="PLW80_11200"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPB67125.1"
                     /translation="MIKAFLFDLDGTLVDTLGDIASIMNGFLRSKGWPEHPTEAFRTM
                     VGRGFANLVRSATPAEASADLERLYQEAFVLYEAAGAGESRPYPGTIEALRSLSDAGA
                     ALAVVSNKPEPIAARVIGALFPGLRFALVRGGRDGQPFKPDPTGALEAATACGVTPRE
                     CAFVGDSDVDMRTAVAAGMLPVGAAWGFRGEAELRDAGAVVIAASMADVP"
BASE COUNT          163 a          394 c          371 g          160 t
ORIGIN      
        1 ctcgcctcat cgggcaggta gcggtcgagc acgtcggata ggcggcggcg cctgtcgctc
       61 gacagctcgt tggccagaag ccgcgcgctc ggccccatcc gcgacgcgat gaccaggctc
      121 ttgccgcccg gggcggagca ggcgtcgaga atctcgcccg agtcgggcag gagcaggcga
      181 tgagcggcgg ctaccgaggc cgagtcgagc cgatacggct cccgaaggcc gtcggcgtag
      241 tcgatcgcgt cgccgggcgc gaggagcgcc tcgcgtaggc cgtcccatcg tcccccgaac
      301 agttcccgaa agaatcggtc gaaggcctcg cccccgccgt ccgtctgctt tcccatgagc
      361 ccctcctccg cgatacccga gccagagcca cggttgaccg cctacggcat aatccgtact
      421 cttagcggca catacctacc ataaggatcg aacgcccatg atcaaggctt tcctattcga
      481 cctcgacggc accctggtcg acacgctcgg cgacatagcg tcgatcatga acggcttcct
      541 gcgctccaaa ggttggcccg agcacccgac cgaggcgttc aggaccatgg tcggccgcgg
      601 gttcgcgaac ctggtacgga gcgccacgcc ggcggaggcg tcggccgacc tggaacgatt
      661 ataccaggaa gccttcgtcc tgtacgaggc ggccggcgcc ggagaatccc gtccctaccc
      721 cgggaccatc gaggcgctgc ggtcgctgtc cgatgccgga gcggccctgg ccgtcgtctc
      781 gaataagccc gagcccatcg cggcgcgcgt gatcggcgcc ctgttccccg gccttcgctt
      841 cgcgctcgta cgcggcggcc gcgacggtca gccgttcaag ccggacccga cgggagcgct
      901 cgaggccgcg acggcctgcg gcgttacccc ccgcgagtgc gccttcgtcg gcgactcgga
      961 cgtggacatg aggacggccg tggcggccgg catgctgccc gtcggggcgg cctggggctt
     1021 ccgcggcgag gcggagctac gcgacgccgg ggccgtcgtg atcgccgcct cgatggccga
     1081 cgtgccgc
//
LOCUS       DAOOLN010000964         1087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_183975_length_1087_cov_1.960271, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000964 DAOOLN010000000
VERSION     DAOOLN010000964.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183975_length_1087_cov_1.960271"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1087
                     /locus_tag="PLW80_11205"
     CDS             <1..>1087
                     /locus_tag="PLW80_11205"
                     /inference="COORDINATES: protein motif:HMM:NF026042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HPB67126.1"
                     /translation="AYLIEEKGFDTYVKLNPTLLGYDEVRDILDATGWRRIGLKRPTF
                     EHDLQFDAALALIASLRAKAKAKGVRFGVKLSNTLANVNDGKRMPGGERYMSGRALFP
                     ITARLAARLAAALPEPLPFSYCGGVSAHNAFDCMQAGLGPLTVATDILKPGGYLRLEP
                     IAREAARSLAAGVPAAPDAARLDALAKAALTDAAYRGDAKKGEAVIKKSLPQTDCFAA
                     PCVEACPAAQKPPAYMKALAAGDAQKALKIVLADNPLPHVTGVLCDHQCMYACSRVDY
                     EGPVEIRDMKLACARAATVPAVKKPAISDKGKTAVFGAGPAGLACAHYLALEGYPVTV
                     FDTSSGPGGVPANVIPRCWHKRRRRLKR"
BASE COUNT          195 a          395 c          339 g          158 t
ORIGIN      
        1 gcgtatttaa tagaagaaaa gggcttcgac acgtatgtca agctcaaccc caccctgctc
       61 ggctacgacg aggtacgcga catcctcgac gctaccggct ggcggcgaat cggcctcaag
      121 cgcccgacct tcgagcacga cctgcagttc gacgcggccc tcgcactgat cgcctcgcta
      181 cgcgccaagg ccaaggccaa gggcgtacgc ttcggcgtca agctatccaa caccctggcc
      241 aacgtgaacg acggtaagcg gatgcccggc ggcgagcgct atatgtcagg ccgcgcccta
      301 ttcccgatca ccgcgaggct cgcggccagg ctggccgcgg cgttgcccga gccgctaccg
      361 ttctcgtact gcggcggcgt cagcgcccat aacgccttcg actgcatgca ggccggcctc
      421 gggcccctga ccgtcgccac cgatatccta aagccaggtg gatacctccg cctcgagccg
      481 atagcccggg aagccgcccg ttcgctcgcg gccggcgtac ctgcggctcc cgacgcggcc
      541 cgcctcgacg cgctcgccaa ggcggcgctg acggacgcgg cctatcgtgg cgacgccaag
      601 aagggcgagg ccgtcatcaa gaagagcttg ccgcagaccg actgcttcgc cgcgccctgc
      661 gtcgaggcct gccccgccgc gcagaaaccg ccagcctata tgaaggccct tgcggccggc
      721 gacgcccaga aggccctgaa gatagtcctc gccgacaatc ccctgcctca tgtcaccggc
      781 gtgctatgcg accaccagtg catgtacgcc tgctcacgcg tcgactatga gggccccgtg
      841 gagatccgtg atatgaagct ggcctgcgcc cgagccgcga ccgtcccggc cgtcaagaag
      901 ccggcgatat ccgataaggg caagacggcg gtcttcggag cggggccggc cggcctcgcc
      961 tgcgcccact acttggcgct cgagggctat ccggtaaccg tgttcgatac ctccagcggg
     1021 cccggcggcg tgccggcaaa cgtgatcccg cgctgctggc acaaaaggcg caggaggctg
     1081 aaaaggc
//
LOCUS       DAOOLN010000965         1083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_185003_length_1083_cov_2.324903, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000965 DAOOLN010000000
VERSION     DAOOLN010000965.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185003_length_1083_cov_2.324903"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PLW80_11210"
     CDS             complement(<1..991)
                     /locus_tag="PLW80_11210"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPB67127.1"
                     /translation="MVHSLLKIGAAAIVSVALASLLSSCWYAKQAAHFLAERASAVPV
                     ERVASNPGTPPETVAMLETVDRVRRFSVDRVGLSPTRNYTRYVALDRDYVADVVSACA
                     ADSFERYRWRYPVLGELPYKGFYDKADANREAKRLKEAGLDVIVRQVEAFSSLGYFKD
                     PLYSFMVGSDEDELAELVIHESAHATLFVKGADQFNEGFATFVGRRGAELYLDERYGP
                     DSTQAARRRARMADGRAFVGFIRGTAALLEEAYADESVGRDARLERKAAVIAERASLF
                     AAERERLFDGEAYRRFDMSTINNAYIDLYRLYEDDLSLFDAWFDREAGGSLPVF"
BASE COUNT          204 a          352 c          348 g          179 t
ORIGIN      
        1 cgaatacagg tagcgatcct ccggcttccc ggtcgaacca ggcgtcgaag aggctcaggt
       61 cgtcctcgta gagccggtag aggtctatat aggcgttgtt gatcgtcgac atgtcgaagc
      121 gacgataggc ctcgccatcg aataggcgct cccgctcggc ggcgaacagc gacgcccgct
      181 cggctatcac ggcggccttg cgctcgagcc tcgcgtcgcg gccgaccgac tcgtcggcgt
      241 atgcctcctc gagcagagcg gccgtaccgc gtataaaacc gacgaaggca cgcccgtcgg
      301 ccatcctcgc tctccgcctc gcggcctggg tcgagtccgg gccgtagcgc tcatcgaggt
      361 acagctcggc gccgcgccta ccgacgaagg tggcgaagcc ctcgttgaac tggtctgccc
      421 ccttgacgaa cagggtcgcg tgcgccgatt cgtggatcac cagctcggca agctcgtcct
      481 cgtcgctccc gaccatgaac gaatagagcg gatccttgaa gtatccgagc gagctgaagg
      541 cctcgacctg cctgacgatg acgtcgagcc ccgcctcctt gagccgcttc gcctcgcggt
      601 tagcgtcggc cttgtcgtag aaacccttgt acggtagctc gccgagtacg ggatatcgcc
      661 agcgatagcg ttcgaaggag tctgccgcgc aggcagagac gacgtccgcc acgtagtcgc
      721 ggtcgagagc cacgtagcgc gtgtaattcc tggtagggga caggccgacg cggtccacgg
      781 agaaacgcct gacccgatcg accgtctcga gcatggcaac ggtctcaggc ggtgtgcccg
      841 gattggaggc gacccgctcg accggcacgg ccgaggcgcg ctcggccagg aaatgcgccg
      901 cctgtttggc gtaccagcac gacgatagga gggacgccag ggcgacggag acgatagccg
      961 cggcgcctat cttgagtagc gaatgaacca tagccgattg tagcaccgtt caggacacct
     1021 tgcacccgtc tgaacggcgc tgatagactc ccatcatggc acgtaaggaa cagccgaccg
     1081 gag
//
LOCUS       DAOOLN010000966         1082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_185256_length_1082_cov_2.387537, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000966 DAOOLN010000000
VERSION     DAOOLN010000966.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185256_length_1082_cov_2.387537"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PLW80_11215"
     CDS             <1..338
                     /locus_tag="PLW80_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67128.1"
                     /translation="LRVPEAAVLDPGVEELPVSLGLSVRSETASSLGMNAGPPAEYCP
                     NKAHVGTLLALADPLGLRRRDILPLLDTVRERGLRLEGGLIGRLCYIVDAEGGRLYYF
                     SVNLCVVSA"
     gene            411..>1082
                     /locus_tag="PLW80_11220"
     CDS             411..>1082
                     /locus_tag="PLW80_11220"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPB67129.1"
                     /translation="MKMRRTYRTLVAAIAILVSVLAGCNSVGRGGSVYAPGTYSVSTK
                     GFGGDVTVALTVDARSIVGVEIVGEDETPEIGGMAMERMARDIPAVGEAVEGVSGATY
                     TSDAVKRALSEALARARGESVSVKLDVAPGSYGASAPGFDLSVPVPVTVGVADGRIAS
                     IEIGACKETEGKLTAVRDRLIPRIIERQSLAVDAVTGATATSNAVIGAVRASLVAAGA
                     SEAALY"
BASE COUNT          166 a          340 c          387 g          189 t
ORIGIN      
        1 cgctacgggt gccggaggcc gccgtactcg atccgggcgt cgaggagctt ccggtctccc
       61 tcgggctcag cgtgcgctcg gagacggcgt cgtccttagg gatgaacgcc ggcccgccgg
      121 cggagtactg cccgaacaag gctcacgtcg gtaccctcct ggctttggcc gacccgttgg
      181 gcctacggcg gcgggacatc ctacccctcc tggataccgt ccgggaacgc gggctcaggc
      241 tcgagggcgg cctgatcggc aggctgtgct atatcgtcga cgcggaaggg ggacgactct
      301 attacttctc ggtcaacctc tgtgtagttt ccgcttgacc gtgtggttac cacgcgcatt
      361 acgatcccat tcaggcggat agcctcagta ccgttataag gggatccatg atgaagatgc
      421 gaaggacgta tcggacgctg gtcgcggcga tcgcgatatt ggtttcggtc ctggccggat
      481 gcaatagcgt cgggcgtggg ggctcggtat acgccccggg cacgtactcc gtgtcgacca
      541 agggcttcgg cggtgacgtg accgtggcgc tcacggtcga cgccaggtcc atcgtcggcg
      601 tggagatagt cggcgaggat gagacgccag agatcggcgg catggccatg gagcgtatgg
      661 cccgcgatat ccctgccgtc ggcgaggcgg tcgagggcgt cagtggcgcc acgtacacga
      721 gcgacgccgt caagcgggcg ctctccgagg ctctggcgcg ggcccgcggc gagagcgttt
      781 ccgtcaagtt ggacgtcgcg ccgggtagct atggcgcgtc ggcgccgggc ttcgacctct
      841 ccgtcccggt gcccgtgacg gtcggcgtcg cggacggccg gatcgcttcg atagagatag
      901 gcgcgtgcaa ggagaccgag ggtaagctca ctgcagtgcg cgaccgcctg ataccgcgca
      961 tcatcgagcg gcaatcgtta gccgtcgacg ccgtcaccgg ggccacggcc acgtccaacg
     1021 cggtcatcgg agccgtacgg gctagcctcg tcgcggccgg agcgtccgag gcggccctgt
     1081 ac
//
LOCUS       DAOOLN010000967         1081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_185461_length_1081_cov_3.114035, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000967 DAOOLN010000000
VERSION     DAOOLN010000967.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185461_length_1081_cov_3.114035"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..373)
                     /locus_tag="PLW80_11225"
     CDS             complement(119..373)
                     /locus_tag="PLW80_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPB67130.1"
                     /translation="MQAAPVVIAICMGSSCYARGNARNAELIKAWLAREGRDARIEFN
                     GTLCEGLCKEGPNLRIGDRVYRGVDPAAVEDILEHEFGER"
     gene            complement(444..794)
                     /locus_tag="PLW80_11230"
     CDS             complement(444..794)
                     /locus_tag="PLW80_11230"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HPB67131.1"
                     /translation="MTIDSATLAYQNRSLQVPRAPAQGLGARSASGGTMIDRSSKLYE
                     QCQEFESIFVNMMLKEMRGTVEKSGLIDGGQAEEIFSDMLYDEYAKDMSKNAGFGLAD
                     AVYLELSRQSYRAG"
     gene            complement(801..>1081)
                     /locus_tag="PLW80_11235"
     CDS             complement(801..>1081)
                     /locus_tag="PLW80_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HPB67132.1"
                     /translation="GRLELYRFPNPVGLTAVGENLFKQSNATGDPIPGRPGFDGMGKT
                     VHKFLEMSNVSVVKEMVNMIVAQRAYEFNSKAIQTSDNMLGTATSLKR"
BASE COUNT          175 a          327 c          359 g          220 t
ORIGIN      
        1 acgacgctgg ccttgccctc gcagaccctg atggccttga ccggacactc gcgcacgcac
       61 ttgtagcagt cctggcaggc cgcgctctcg gtgcggatgg gttcgtagtt gggcatggtc
      121 aacgctcccc gaattcgtgc tccaggatgt cttccaccgc cgcggggtcc accccgcggt
      181 agacgcggtc gccgatgcgc aggttggggc cttccttgca gaggccctcg cagagggtcc
      241 cgttgaattc gattctagcg tcgcggccct cgcgcgccaa ccaggccttg atcagctcgg
      301 cgttgcgggc gttgcccctg gcatagcacg atgatcccat acagatggcg atgacgacgg
      361 gggctgcttg catggactaa taatatataa atatatcgat aatgtcaatc gaaagaccgg
      421 gcgagcgcgg ctacggacgg ctctcagccg gcccggtagg attgcctgga cagctcgagg
      481 tagacggcgt cggcgagccc gaagccggcg ttcttcgaca tgtccttggc gtattcgtcg
      541 tagagcatgt cgctgaagat ctcctcggcc tggccgccgt cgatcaggcc agacttctcg
      601 acggtccctc gcatctcctt gagcatcatg ttgacgaaga tggactcgaa ctcctggcac
      661 tgctcgtaca gcttgcttga gcggtctatc atcgttccgc ccgaggcgga gcgggcgccg
      721 aggccctggg ccggggcgcg gggtacctga agcgagcggt tctggtaggc cagcgtggcg
      781 ctatcgatgg tcacggagcg ttacctcttg aggctggtgg cggtgccgag catgttgtcg
      841 ctcgtctgga tggccttgga gttgaactcg taggcgcgct gggcgacgat catgttgacc
      901 atctccttga ccaccgacac gttcgacatc tccaggaact tgtggaccgt cttgcccatg
      961 ccgtcgaagc ccgggcggcc ggggataggg tcgccggtgg cgttcgactg cttgaacagg
     1021 ttctcgccga cggcggtcag gccgaccggg ttcgggaagc ggtacagctc taggcggccg
     1081 a
//
LOCUS       DAOOLN010000968         1081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_185498_length_1081_cov_2.631579, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000968 DAOOLN010000000
VERSION     DAOOLN010000968.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185498_length_1081_cov_2.631579"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..>1081)
                     /locus_tag="PLW80_11240"
     CDS             complement(78..>1081)
                     /locus_tag="PLW80_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HPB67133.1"
                     /translation="SVNRAHRPTGGAKVGPEIVETLNATVVKTAGVTVYTKTRATAIL
                     KDKSGAVTGIKVIAADGREYDIKAKAVIIATGGFGANNDMAASYVPSLKGFATTNHPG
                     ATGDGILLAEAAGAALVDMKEIQTHPTHAPDKEMIIEAVRGNGAILVSKEGARFVDEL
                     ATRDVVSAAVLAQTGKTAFLVFDQSIRASLKAIEGYAKLGIVVEGDTPEALVAKIGSD
                     PARLAATLAAYNGFVAAKRDAEFGRADMPRAVSVGPFYAIEITPAIHHTMGGVKIDAE
                     ARVIAASGSPIVGLWAAGEVTGGVHGGNRLGGNALADIVTFGRIAGNGAAAYATSSR"
BASE COUNT          144 a          337 c          411 g          189 t
ORIGIN      
        1 cgcggcgaga actagccact gacaagcaca atgggccgcc ccgacggggc ggcctcggta
       61 tcagacgggt ctagcgctta gcgtgaagac gtggcgtagg ccgcggcgcc gttaccggct
      121 atgcggccga aggtgacgat gtcggcgagc gcgttgccgc cgaggcggtt gcctccgtgg
      181 acgccgccgg tgacctcgcc cgcggcccag aggccgacga tcggggagcc ggaggcggcg
      241 atgacgcggg cctcggcgtc gatcttgacg ccgcccatcg tatggtggat ggccggggtt
      301 atctcgatgg cgtagaacgg cccgacggac acggccctgg gcatgtcggc gcggccgaac
      361 tcggcgtcgc gcttggcggc gacgaagccg ttataggcgg cgagcgtggc ggccagccgg
      421 gcggggtcgg agcctatctt ggcgaccaga gcctcggggg tgtcgccctc tacgacgatg
      481 ccaagcttcg cgtagccctc tatggccttg aggctggcgc gtatcgactg gtcgaacacg
      541 aggaaggcgg tcttgccggt ctgggcgagc acggccgcgg acacgacgtc gcgggtagcc
      601 agctcgtcca cgaagcgggc gccttccttg ctgacgagga tggcgccgtt gccgcgtacg
      661 gcctcgatta tcatctcctt gtcgggggcg tgcgtcgggt gggtctggat ctccttcatg
      721 tcgacgaggg ccgcgccggc ggcctccgcg aggagaatgc cgtcgccggt ggcgccgggg
      781 tggttcgtcg tcgcgaagcc cttgagggac ggcacgtagg acgcggccat gtcgttgttg
      841 gcgccgaagc cgcccgtggc gatgatgacg gccttggcct tgatgtcgta ctcgcgtccg
      901 tcggcggcta tcaccttaat accggtgacg gcgcccgact tgtccttgag gatagcggtc
      961 gccctggtct tggtgtacac cgtgacgccg gccgtcttga cgacggtcgc gttaagggtc
     1021 tcgacgatct ccggtccgac cttggcgccg ccggtgggcc tgtgcgcgcg gttgacgctg
     1081 g
//
LOCUS       DAOOLN010000969         1080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_185727_length_1080_cov_3.099512, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000969 DAOOLN010000000
VERSION     DAOOLN010000969.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185727_length_1080_cov_3.099512"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PLW80_11245"
     CDS             <1..330
                     /locus_tag="PLW80_11245"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPB67134.1"
                     /translation="RGDLYDDAVKAAIAERGPYDLVLSDAAPATSGNRGLDTDRSEAI
                     VEAVLGYADDMLKPGGSVVAKLFIGGGQRELLDRMKASYASARAFKPEACRTVSFETY
                     LIGLEKK"
     gene            complement(335..793)
                     /locus_tag="PLW80_11250"
     CDS             complement(335..793)
                     /locus_tag="PLW80_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPB67135.1"
                     /translation="MDLSSLITRGGVWYNVPGKDPAEFVEALAATLRLPDGVSPNDLA
                     MACARREANSPTAMGRGLAFPHPGTPLIADAGRAMVALAYSRFPVDWKAPDGAPVRAA
                     FLILSASRNEHLTTLSALAKLCGDGEFYAALGRRAPLEDIVALLGKKTPA"
BASE COUNT          188 a          376 c          360 g          156 t
ORIGIN      
        1 cgcggcgacc tctacgacga cgcggtcaag gcggccatag ccgaacgcgg cccgtacgac
       61 ctcgtgctgt cggacgccgc ccccgcgacg agcggcaacc ggggcctgga caccgaccgg
      121 tccgaggcca tcgtcgaggc ggtgctaggc tacgccgacg acatgctcaa gccaggggga
      181 tccgtcgtcg ccaagctgtt cataggcggc ggccagcgcg agctcctgga ccgcatgaag
      241 gcctcgtacg cgagcgcccg cgccttcaag ccggaggcct gccgcacggt cagcttcgag
      301 acctacctga tagggctcga gaaaaagtag actctcaggc cggggtcttc ttcccgagca
      361 gagcgacgat atcctcgagc ggcgcccgac gcccaagcgc cgcgtagaat tcgccgtctc
      421 cgcatagctt ggccagcgcc gagagggtcg tcaggtgctc gttgcgcgag gccgacagga
      481 tcaggaaggc ggccctgacc ggcgctccgt cgggagcctt ccagtccacc gggaaacggg
      541 aatacgcgag cgcgaccatg gcccggcccg cgtcggctat caggggcgta ccggggtgcg
      601 gaaaggccag cccccgcccc atcgcggtcg gcgagttcgc ctcgcgcctg gcgcaggcca
      661 tggccaggtc gttcggcgac acgccgtcgg gtagcctgag cgtagcggcc agcgcttcga
      721 cgaattcggc cggatccttg cccggtacat tgtaccagac ccctccccgg gtaatcagcg
      781 acgataggtc cattccctct cctgccagcg gccacctgcg gctcgaccgc tcggcctcaa
      841 gtataccgac gaggcgacga atggagaata gcgtttcggt cagccatggg gctgcgccgc
      901 ggtatcgcgg agatatcgcg gcgccacccc gaacgtcccg gttaacgctc caggcgcgcg
      961 tagatcgtgg cggataggcc cggcatcggg tagcccgtat actcgtagta agcctcgtcg
     1021 agcaggttgt ccaggcggaa gccgacgaaa tagccatcga gcgcctccca ctcggccgcc
//
LOCUS       DAOOLN010000970         1080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_185745_length_1080_cov_2.808780, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000970 DAOOLN010000000
VERSION     DAOOLN010000970.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185745_length_1080_cov_2.808780"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PLW80_11255"
     CDS             complement(<1..675)
                     /locus_tag="PLW80_11255"
                     /inference="COORDINATES: protein motif:HMM:NF040244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HPB67136.1"
                     /translation="MRYENIGRHASRFKEYVELRIQENRSIGISLLNGDVVGNARGAQ
                     TGLSARAFKNGVWGFASSPELDDGAIRAAVDRAVENARFLGAREAAGALTKGPLPSRP
                     GRGDYGLGTAEPRKSQKDLIEFVRSIDAYCQKYYPGLSSRSVSLSCLDMEKTILTADG
                     AEAYSMTPRTIIAVSLTVEEGGEPIDLYEVYGGRGQFEDVFEDPRDLYPNIDALADNL
                     ARKSKGV"
     gene            836..>1080
                     /locus_tag="PLW80_11260"
     CDS             836..>1080
                     /locus_tag="PLW80_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67137.1"
                     /translation="MIDPTDPSSAFAGLRDRDYARAGLMVCEGRIVVQKALDCGVRLR
                     SMLCVPADKEEWLGVSGGRFPVAALSRADMADLLGFQ"
BASE COUNT          151 a          361 c          372 g          196 t
ORIGIN      
        1 gacgcccttg gacttgcggg ccaggttgtc ggccagcgcg tcgatgttcg ggtacaggtc
       61 gcgcggatcc tcgaacacgt cctcgaactg gccgcggccg ccgtagacct cgtataggtc
      121 gatcggctcg cctccctcct cgacggtcag cgataccgcg atgatcgtcc ggggcgtcat
      181 cgaataggcc tcggcgccgt cggcggtcag gatggtcttc tccatgtcga gacaggacag
      241 gctgaccgag cgcgacgata gcccgggata gtacttctgg cagtacgcgt cgatggagcg
      301 gacgaactcg atcaggtcct tctggctctt gcggggctcg gcggtgccga ggccgtagtc
      361 gccgcgaccc ggcctggacg gtagcgggcc cttcgtcagg gcgccggccg cctccctggc
      421 gccgaggaag cgggcgttct cgaccgcccg gtcgacggcc gctcggatcg cgccgtcgtc
      481 gagctcgggc gacgaggcga agccccagac accgttcttg aaggcgcggg ccgacagccc
      541 cgtctgagcg ccgcgcgcgt ttcctacgac gtctccgttg agcaggctga tgcctatcga
      601 gcggttctcc tggatgcgta gctcgacgta ttccttgaac ctggacgcgt ggcgccctat
      661 gttctcgtat ctcatgcggc ctcccgggca ggtgatcgaa acgccccgag ggggcgcgcc
      721 cgcgaagggc gtaggatact aaccatactt gcatagcccg gcgagggcaa gggtcggctt
      781 cggccaatgc ggcaggcctg tcgccctagc gcctcgattt tggtatactc cggcgatgat
      841 cgatcctacc gacccgtcct cggccttcgc cggcctgcgc gaccgggact acgcccgcgc
      901 cgggctgatg gtctgcgaag gacgtatcgt cgtccagaag gccctcgact gcggcgtacg
      961 gctgcgctcg atgctctgcg tgccggccga caaggaggag tggctcggcg tgtcgggcgg
     1021 tcgcttccct gtggccgcgc tgagccgtgc cgacatggcc gacctgctcg gcttccagtt
//
LOCUS       DAOOLN010000971         1079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_186082_length_1079_cov_2.344727, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000971 DAOOLN010000000
VERSION     DAOOLN010000971.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186082_length_1079_cov_2.344727"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PLW80_11265"
     CDS             complement(<1..193)
                     /locus_tag="PLW80_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HPB67138.1"
                     /translation="MEHYDVAIIGTGPAGLGAAFSLLEARPDFRIALVDRNQYSSGGL
                     RNDCKMNFSWPVGFPEDDWD"
     gene            complement(231..980)
                     /locus_tag="PLW80_11270"
     CDS             complement(231..980)
                     /locus_tag="PLW80_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67139.1"
                     /translation="MAEARRLASLAAAMLFAASAMVGADPAPSGWAPGAADSARRAPA
                     GTPPEAAAALLYGVPDGLALASIVGKPTLLGTVNYLFKDGDERQLAGYVDVVAVYDLP
                     LEAMAAALVDFEAAPGYVPHILAATVRRRGPDGVEIYYKSGFRVLGIEISYESVFLTA
                     VDRLDGGAVGIRSLMLDSLDDRSFEHYTSTYLAPVVVDGRPMTFVRYFNRPGLRKPGL
                     GLLQVLNLFVSPEAKAQLEALEGETRRRLER"
BASE COUNT          172 a          367 c          384 g          156 t
ORIGIN      
        1 ggtcccagtc gtcctccggg aagccgaccg gccagctgaa attcatcttg cagtcgttgc
       61 ggaggccgcc cgaggagtac tggttgcggt cgacgagggc gatgcggaag tcgggccgcg
      121 cctcgaggag ggagaatgcc gcgccgagtc cggcggggcc ggtgccgatg atggctacgt
      181 cgtagtgttc cataccggta ccgcatgcta gcagatgcgc cggccttgta ctagcgctcc
      241 agccgcctgc gggtctcgcc ctcgagcgcc tcgagctggg ctttggcctc cggcgagacg
      301 aacaagttga gcacctggag gagcccaaga cccggcttgc gcaggccggg ccggttgaag
      361 tagcgaacga aggtcatcgg cctgccgtcg acgacaaccg gcgccaggta ggtggacgta
      421 tagtgctcga aggagcggtc gtccaggctg tcgagcatca ggctccgtat cccgaccgcc
      481 ccgccgtcca gccgatccac ggccgtcagg aagaccgact cgtagctgat ctcgatgccg
      541 aggacgcgga agcccgactt gtagtatatc tcgacgccgt cggggcctcg tcgccggacg
      601 gtcgccgcga gtatgtgcgg cacgtagccg ggcgccgcct cgaagtccac cagggcggcg
      661 gccatcgcct cgaggggcag atcgtagacg gccacgacat cgacatagcc ggcgagctgc
      721 ctctcgtcgc cgtccttgaa gaggtagttc accgtgccga ggagggtcgg cttgccgact
      781 atcgacgcga gcgcgagccc gtcggggacg ccgtagagca gggccgcggc cgcctccggc
      841 ggggtgccgg ccggagcgcg cctggccgaa tccgcggccc ccggcgccca tccgctcggc
      901 gcggggtcgg cgccgaccat cgcgctggcc gcgaacagca tcgcggcggc cagcgaggcg
      961 aggcgccgcg cctcggccat cgctagctcc cgcccccggc tatgatgccg aagacccggt
     1021 ccaggtcgtc catcgagaaa taatctatcg tgatcgagcc gcgcgaggcg tcaccccgg
//
LOCUS       DAOOLN010000972         1078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_186381_length_1078_cov_2.457478, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000972 DAOOLN010000000
VERSION     DAOOLN010000972.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186381_length_1078_cov_2.457478"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PLW80_11275"
     CDS             complement(<1..649)
                     /locus_tag="PLW80_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67140.1"
                     /translation="MKRLRLKTYLIVLPIIAATVVVSGFVAYQESRSSLTRLASRHLA
                     YKAEQLRDFINSEWSVVESLGLGDDQAYRKAQEASFRSYAYALLRSDSEAIAVYGDDG
                     RVRFIVSGSSSEDEGAFPMPGTTAGWFSSSSRSGSRVGIAFPFEPFGWTVALSESADY
                     FYADERDLLRGFGWILSIALGAAVLLTAVYLRRVTGPLERLSDAVEDLRGYTAQVA"
     gene            751..>1078
                     /locus_tag="PLW80_11280"
     CDS             751..>1078
                     /locus_tag="PLW80_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67141.1"
                     /translation="MFVLNAVIVTLAVAIMLVAAARYANDARSETLGSTSYFTTEWRL
                     LRQLKESTDRALSAKDAEIEELRERYQSLQRSGAPASLLRSIDEAIREAEEGRAARKS
                     PTSWPGA"
BASE COUNT          201 a          327 c          360 g          190 t
ORIGIN      
        1 cggcgacctg cgcggtgtat ccccggagat cctcgacggc gtcgctcagg cgctcgagcg
       61 gccctgtgac ccggcgtagg tagaccgcgg tcaggagcac ggccgcgccg agcgcgatcg
      121 aaaggatcca gccgaagccg cggaggagat cccgctcgtc ggcgtagaaa tagtcggcgc
      181 tctcgctgag cgcgacggtc cagccgaacg gctcgaacgg gaaggctatg ccgacgcggg
      241 accccgaacg ggacgacgag gagaaccatc ccgccgtcgt tccgggcata gggaaggccc
      301 cctcgtcctc ggaactggag ccgcttacaa tgaagcgcac gcggccatcg tcaccgtaga
      361 cggctatcgc ttcgctatcg ctacgcaaga gcgcgtacgc gtaggagcgg aacgaggctt
      421 cttgtgcctt gcggtacgcc tggtcgtcgc cgagcccgag cgactccacc accgaccatt
      481 cgctgttgat gaagtcgcgc agttgctccg ccttgtaggc caggtggcgg ctggcgaggc
      541 gggttagact ggatcggctc tcctgatagg cgacgaagcc gctgacgacg acggtggcgg
      601 cgatgatagg cagtacgatg aggtaggtct tgagtcgtag gcgcttcatg tcgttagcat
      661 tctatagtat tatcataatt ctgaccaatt ggtatcatca cgaggggagc gacgcgtatt
      721 ttgactagcc ataggcgggg cgattattcg ttgttcgtac tcaacgccgt catcgtcacg
      781 ctagcggtag ccatcatgtt agtcgccgcg gctcgttacg ccaacgacgc gcgttctgaa
      841 accctcggct cgacgagcta ttttacaacc gagtggcggc tgcttcggca gctcaaggag
      901 tcgactgaca gggccttgtc cgccaaggat gccgagatcg aggagctgcg cgagcgctac
      961 cagtccctcc agcgctccgg cgcgccggcc agcctgctgc ggtccatcga cgaggcgata
     1021 cgtgaggcgg aggaaggccg cgcggccaga aagtcgccga ccagctggcc gggggctt
//
LOCUS       DAOOLN010000973         1077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_186663_length_1077_cov_2.411937, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000973 DAOOLN010000000
VERSION     DAOOLN010000973.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186663_length_1077_cov_2.411937"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PLW80_11285"
     CDS             complement(<1..570)
                     /locus_tag="PLW80_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67142.1"
                     /translation="MGLTLFTYLAAILAAAAAIAVKGARPGLRAFRAAAFFSVAYALL
                     LATGYMLSIDYPAAALVLVRAAGAGIIASYLAFLRLAIGYPYAKRLFILDLALVAIGL
                     AAVWAFVFTDAYIVEVKRFELEYIRFEGDYHRPLSLVGLAAGLAAALTMTARSFITKS
                     RVYRQHLAVMAAGLLFSVAWGYVFAIVGPT"
     gene            complement(561..>1077)
                     /locus_tag="PLW80_11290"
     CDS             complement(561..>1077)
                     /locus_tag="PLW80_11290"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HPB67143.1"
                     /translation="TIRAAGDKADLAALIRSCDRAIRGMGLEDKYVVMFLGLVDVGAK
                     RLRYVNAAMPDQYLVVRTVKGPVIKRLASNCGIVGLVPVASVEVEEIELRTDDVIALT
                     TDGLTEIMGADGLAIDQSTEFAKLMGGAVDSSIDDLVERLATLGETYNAEASLRDDIT
                     ILAAKVGRLWD"
BASE COUNT          197 a          357 c          364 g          159 t
ORIGIN      
        1 cgtcggcccg acgatcgcga agacgtagcc ccaggcgacg ctgaacagga ggcccgcggc
       61 catcacggcg aggtgctgac gatagacgcg gctcttcgtg atgaacgacc gcgcggtcat
      121 ggtcagggcc gcggccagcc cggccgcgag gccgaccaag gacaacggcc ggtggtagtc
      181 gccctcgaag cgaatgtact cgagttcgaa gcgcttgacc tcgacgatgt acgcgtccgt
      241 gaacacgaag gcccaaacag ccgccagccc gatggctacc agcgccaggt cgagtatgaa
      301 gagccgcttc gcgtacgggt agcctatggc caggcgcagg aacgccaggt acgaggcgat
      361 gataccggct cccgccgcgc gtacgagcac aagcgccgcc gcggggtagt cgatggacaa
      421 catgtagccc gtagccagaa gcagggcata cgctacgctg aagaacgccg ccgcgcggaa
      481 cgcccggagc ccgggtcgag cgcctttgac ggcgatggcg gccgccgcgg cgagtatcgc
      541 cgcaaggtag gtaaaaagcg tcagtcccat aaccgtccaa ccttggccgc cagtatcgtg
      601 atatcgtcgc gtagcgacgc ctcggcgttg taggtctcgc cgagggtggc taggcgctcg
      661 acgaggtcgt ctatgctcga gtcgacggcg ccgcccatca gcttggcgaa ctccgtcgac
      721 tggtcgatcg ccagtccgtc ggcgcccatt atctcggtca gcccgtcggt cgtcagcgcg
      781 atgacgtcgt cggtccgcag ctctatctcc tcgacctcga ccgaggccac cggtacgagg
      841 ccgacgatgc cgcagttcga ggcgaggcgc ttgatgacgg gccccttgac ggtgcgcacg
      901 accaggtact ggtccggcat ggccgcgtta acatagcgca ggcgcttggc cccgacgtcg
      961 accaggccca ggaacatcac cacgtacttg tcctcgaggc ccatgccgcg gatggcccga
     1021 tcgcaggagc ggatgagcgc ggccaggtcg gccttgtcgc cggccgcgcg tatcgtg
//
LOCUS       DAOOLN010000974         1076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_186899_length_1076_cov_2.763957, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000974 DAOOLN010000000
VERSION     DAOOLN010000974.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186899_length_1076_cov_2.763957"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="PLW80_11295"
     CDS             complement(<1..826)
                     /locus_tag="PLW80_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HPB67144.1"
                     /translation="MPRKNDSWNSKKPKKPLKGAPIPPINGWKYTIAYVCIAVISITL
                     FNWFVESADSSIVPYSEFKSLVASGQIERIEMDSNYYTGYGPTSARQDGRPSGQSDGR
                     VFKTVPIYDVEFTKLLDEKGVTYSAVSREGNAMLGFVLNWVLPIGLMFFLWRMLQKRL
                     TGMGSNVLSVGQNKAVIVAEGDVKTRFLDVAGVDEAKEELVEVVDFLKNPKKYTDIGG
                     KIPKGVLLVGPPGTGKTLLARAVAGEAGVPFFRMSGAEFVEMFVGVGAARVRDLFKQ"
     gene            970..>1076
                     /locus_tag="PLW80_11300"
     CDS             970..>1076
                     /locus_tag="PLW80_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPB67145.1"
                     /translation="MKVNPLGDRVLIKVQEGDTKIASGIIIPQTAQEKT"
BASE COUNT          208 a          344 c          301 g          223 t
ORIGIN      
        1 cctgcttgaa gaggtctcgc acgcgggccg cgcctacgcc tacgaacatc tcgacgaatt
       61 ccgcgccgct catgcggaag aacggtacgc ctgcctcccc ggcgacagct cgcgcgagca
      121 gggtcttgcc ggtgcccggt gggccgacga gcagcacgcc cttgggtatt ttgccgccta
      181 tgtcggtgta cttcttggga ttcttgagga agtcgacgac ctcgaccagc tcctccttgg
      241 cctcgtccac acccgccacg tcgaggaagc gcgtcttcac gtcgccctcg gcgacgatga
      301 ccgccttgtt ctgaccgacc gacagcacgt tggagcccat gccggtcagc cgcttctgta
      361 gcatcctcca caggaagaac atcagcccta tcggcaagac ccaattgagc acgaagccga
      421 gcatggcgtt tccctcccgc gacaccgcgg aataggtcac gcccttctcg tcgagcaact
      481 tggtgaattc cacatcgtag atcggcacgg tcttgaagac gcggccgtcg gactgccccg
      541 agggccgacc gtcctgcctg gcgctcgtcg gcccgtagcc ggtgtagtag ttcgagtcca
      601 tctcgatgcg ctcgatctgg cccgatgcga cgagcgactt gaattcgctg tacggcacga
      661 tggagctgtc ggccgactcg acgaaccaat tgaacagggt tatcgagatg acggcgatgc
      721 atacgtaggc gatggtatat ttccagccgt ttatcggggg gataggcgcc cccttcaacg
      781 gtttcttagg cttcttggag ttccatgagt cgttcttacg gggcatcgtt tcctccgacg
      841 tataatgtac gaattcaggc gtcgtatcgg ccagccgcgc ccgcctttct tccttgaccc
      901 ctccatgtcg tttttagtac atttcgtccg tcaacgaaac cactcacaca ggttccagga
      961 ggatacgaca tgaaagtaaa cccgttgggc gatcgcgtgc tcatcaaggt tcaggaaggc
     1021 gataccaaga tcgccagcgg catcatcatc ccgcagaccg cccaggagaa gaccca
//
LOCUS       DAOOLN010000975         1074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_187443_length_1074_cov_3.084396, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000975 DAOOLN010000000
VERSION     DAOOLN010000975.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187443_length_1074_cov_3.084396"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1074)
                     /locus_tag="PLW80_11305"
     CDS             complement(<1..>1074)
                     /locus_tag="PLW80_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB67146.1"
                     /translation="RDEEGRVVAAGFPADEPRSGATSSGAAGTAPAPAGTASAPADSS
                     KASGYERLEREAIAGSHPDYAGLDVSGYLRVVDSPNPMHRLWNDTPWLKYRLAYGCYW
                     RRCAFCDTQLDYIKRYLASDLDALEEAAGRAAGRTGLYGIHFVDEAMPVNLVRAFAER
                     NARRSRPFTFWGNARFDRAWTDEACAFAAERGLVAVSGGIEIATKKGLEIVDKGFSMV
                     DLVRTLASFKRCGVLVHAYLIYGFPGQSDQDIADSAEMARVLLAEGLVDSAFWHRFVL
                     TRHSAIYAAWERGERPGLEPLAPPSSFALNDLRFAGEERSERWTAPLDASLATWIDDG
                     ETDRPLAAFLPRGLPRPRLDVLA"
BASE COUNT          169 a          365 c          390 g          150 t
ORIGIN      
        1 gcgccagcac gtcgaggcgc ggccgcggta agccccttgg caggaaagcg gccagcggcc
       61 tatcggtctc gccgtcgtcg atccaggtgg caagcgacgc gtcgagcggg gcggtccagc
      121 gctcggaccg ctcctcgccg gcgaagcgca gatcgttgag cgcgaaggag ctgggcggcg
      181 ccagcggctc gagcccgggc cgctcgccgc gctcccaggc cgcgtagatc gccgaatggc
      241 gcgtcagcac gaagcggtgc cagaaggccg agtcgacgag cccctcggcg aggaggaccc
      301 gcgccatctc ggccgagtcg gctatgtcct gatcggactg cccggggaag ccgtagatca
      361 ggtaggcgtg cacgagcacc ccgcatcgtt tgaaggacgc cagggtgcgg accaggtcga
      421 ccatcgagaa acccttatcg actatctcca gacccttctt cgtggctatc tcgatgcctc
      481 cggagacggc cacgaggcca cgctccgcgg cgaaggcgca ggcctcgtcg gtccaggcgc
      541 ggtcgaagcg ggcgttgccc cagaacgtga acggcctcga gcgcctcgcg ttgcgctcgg
      601 cgaaagctcg gaccagattg accggcatgg cctcgtcgac gaagtggatg ccatagaggc
      661 cggtccgccc ggccgccctc ccggccgcct cctcgagcgc gtcgaggtcg gaggccaggt
      721 agcgcttgat gtaatccaac tgggtgtcgc agaaggcgca gcgccgccag taacagccgt
      781 aggccagccg atacttgagc catggcgtat cgttccagag gcggtgcatc gggttcggcg
      841 agtcgacgac gcgcaggtaa ccggagacgt cgagcccggc gtaatcgggg tgggagccgg
      901 ctatcgcctc gcgctcgagc cgttcgtagc cgctcgcctt ggacgagtcg gccggcgccg
      961 aggctgttcc ggccggcgcc ggggcggttc cggccgctcc ggaggacgtc gcgccagatc
     1021 gaggctcgtc ggcagggaag ccggccgcga cgactcggcc ctcctcgtcg cggt
//
LOCUS       DAOOLN010000976         1073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_187733_length_1073_cov_2.888016, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000976 DAOOLN010000000
VERSION     DAOOLN010000976.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187733_length_1073_cov_2.888016"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..189)
                     /locus_tag="PLW80_11310"
     tRNA            complement(117..189)
                     /locus_tag="PLW80_11310"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(154..156),aa:Lys,seq:ttt)
     gene            complement(253..>1073)
                     /locus_tag="PLW80_11315"
     CDS             complement(253..>1073)
                     /locus_tag="PLW80_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67147.1"
                     /translation="QADLLGPLSFAYTGKGLGFGLFNRTVATVNAASLLNVAYSVSEE
                     IMLAGGYAYRFELGDDHTIDLGIMPKGFIRASVGDVASLTDIMALVSDPGSLLASPLT
                     MVSGVGFDAGVQWTYRRSLTVGFVAHDAYSPAMVTTYDSYEAFADSPSSGVSAYAVVP
                     AVLSVGAAYDVPWPLLGRLGADLLVLVDYTDLLDLFSALPRNPILNVSFGAELTLLDI
                     LSLRAGVKDALPTAGFGIDLSVFTFSLAMYGEELGLEPGSRPVFNLLVAFEFKY"
BASE COUNT          210 a          324 c          389 g          150 t
ORIGIN      
        1 gccgctttca gcggccacgt caatcgcttc cttgcggaat ccggatatgc gaagcatatc
       61 cggagggcgc gccacagaat tgcctgtccg aaggacagga gcgtcatgga tgacgatgag
      121 ccgcccgggg ttcgaacccg gtacccacgc cttaaaaggg cgttgctcta ccgagtgagc
      181 tagcggctcg tggggcgacc ctgaccatac cctccggcgc ggcaacgtgt caacgccgcg
      241 gcgaggggcg cgtcagtact tgaactcgaa ggcgacgagc aggttgaaca cgggccggct
      301 acctggctcg aggccgagtt cctcgccgta catcgcgagc gagaaggtaa agaccgacag
      361 gtcgataccg aagccggccg tcgggagcgc gtccttgacg ccggcccgta gcgacaggat
      421 gtcgagcagg gtcagctccg cgccgaacga gacgttcagg atcgggttcc tcggcaacgc
      481 ggagaacagg tcgaggaggt cggtgtaatc gacgagcacg aggaggtcgg cgccgaggcg
      541 gcctagtagc ggccagggca cgtcgtaggc ggcgccgacg gacagcacag ccggcacgac
      601 cgcataggcc gacacgccgg agctcgggct gtcggcgaac gcctcgtagc tgtcatatgt
      661 ggtgaccatg gccggcgagt acgcgtcgtg ggccacgaaa ccgaccgtca gcgagcggcg
      721 gtaggtccac tggacgccgg cgtcgaagcc gacgcctgac accatcgtca gcgggctcgc
      781 gagcagcgag ccggggtcgg agacgagggc catgatgtcg gtcagggacg cgacatcgcc
      841 gacgctggcg cggatgaagc ccttgggcat gatgccgagg tctatcgtat ggtcgtcgcc
      901 gagctcgaaa cggtaggcgt agcctccggc gagcatgatc tcctcggaga cggaataggc
      961 gacgttgagc agcgacgcgg cgttgacggt ggccacggtc cggttgaaca ggccgaagcc
     1021 gagccccttg cccgtatagg cgaaggacag cggcccgagc aggtcagcct gga
//
LOCUS       DAOOLN010000977         1073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_187799_length_1073_cov_2.319253, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000977 DAOOLN010000000
VERSION     DAOOLN010000977.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187799_length_1073_cov_2.319253"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..>1073
                     /locus_tag="PLW80_11320"
     CDS             19..>1073
                     /locus_tag="PLW80_11320"
                     /inference="COORDINATES: protein motif:HMM:NF015089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPB67148.1"
                     /translation="MMDGRRGPWLRGVAAQVYADYRRDRVFVVGRLDDGRSFAAEDSS
                     WRPVVYLAAEDAASGESRFGGYRFETCELEAIDGRELVAWTAPGFGAYRDAVAALERA
                     GATVHGSGSIADLYRADLGLALGVRVSGDERPGRRVGLVLADAAVEADPDASAPLSVL
                     SLDIETDESDRSVRAASLAMADWTPSEGRLVPREGSTIVLVVAAPGAAGHAAGPAQGY
                     EAIGRPDEASLIRSLSERVLALDPDVITGWNVVDFDLARLVERAEANGLALDIGRSSD
                     GARVLPKGRGRTATAVVPGRQVIDAMRVARSGPERYDDYSLETVARRALGQGKGSALR
                     GRAKLDWLDRTYRDDPAA"
BASE COUNT          138 a          400 c          394 g          141 t
ORIGIN      
        1 ccatgatagc ctaaacgcat gatggatgga agacggggac cctggctcag gggcgtggcg
       61 gcgcaggtct acgccgacta ccgccgcgac cgcgtcttcg tcgtcggcag gctcgacgac
      121 gggcggtcct tcgccgcgga ggactcgtcg tggcggcccg tcgtctacct cgccgccgaa
      181 gacgccgcgt ccggagaatc ccgcttcggc gggtaccgct tcgagacctg cgagctcgag
      241 gcaatcgacg gtcgcgagct ggtcgcgtgg accgctccgg gcttcggggc ctaccgggac
      301 gccgtcgcgg cgctagagcg ggccggcgcg acggtccacg gctcaggctc gatcgccgac
      361 ctataccgcg ccgacctcgg cctggccctc ggcgtccgcg tctccggcga cgagcggccg
      421 ggccgacgcg tcggcctcgt gctggccgac gcggccgtag aggccgaccc ggacgcctcc
      481 gcgccgcttt cggtcctgtc cctggacatc gagaccgacg agtcggatcg ctccgtccgc
      541 gcggcgagcc tggccatggc ggattggacg ccgtccgagg gcaggctcgt ccctagggaa
      601 gggtcgacga tagtgttggt cgtcgcggcg cccggcgccg ccggccacgc ggccggccct
      661 gcgcagggct acgaggctat cggccgcccc gacgaggcgt ccctgatccg ctcgctatcg
      721 gagcgcgtcc tggccctcga ccccgacgtg atcaccggct ggaacgtcgt cgacttcgac
      781 ctggcccggc tcgtcgagcg ggccgaggcc aacggcctgg ccctcgacat aggccgctcg
      841 agcgacggcg cgagggtact gcccaaggga cgcggccgga ccgccacggc cgtggtgccc
      901 ggccgccagg tcatcgacgc gatgcgcgtc gcgcgctccg ggcccgagcg ctacgacgac
      961 tacagcctcg agacggtagc gcgccgggcg ctcgggcagg gcaagggctc ggccctgcgc
     1021 ggccgggcca agctcgactg gctcgaccgt acctaccgcg acgacccggc ggc
//
LOCUS       DAOOLN010000978         1070 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_188504_length_1070_cov_3.349754, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000978 DAOOLN010000000
VERSION     DAOOLN010000978.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188504_length_1070_cov_3.349754"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PLW80_11325"
     CDS             <1..361
                     /locus_tag="PLW80_11325"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HPB67149.1"
                     /translation="NDALVSAGIGLVIGRITSALGGMKLSVFFSNIVGGAFAAAVAIG
                     AEALRVAGNTDAMIIGSIMLLVPGVLIVNAIRDLIAGDLVAGIARAADAFISAAGISI
                     GVGLALQLAMLISGPTP"
     gene            358..825
                     /locus_tag="PLW80_11330"
     CDS             358..825
                     /locus_tag="PLW80_11330"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HPB67150.1"
                     /translation="MSAQAVQLAWAFLATLGFGVLFRAPLRDLPFTALGGAVSWGAYL
                     AFKAATGSDTLSYFAASVAVGLYAEAMASCLKRPATLFILSAIIPLVPGAGMYRTMLE
                     AVNGRAAASASVGFETLTLAGAIAGGLAVASAASRILWLKKPRLPRRPGSRQP"
     gene            complement(816..>1070)
                     /locus_tag="PLW80_11335"
     CDS             complement(816..>1070)
                     /locus_tag="PLW80_11335"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HPB67151.1"
                     /translation="KMKFVSTVAYVLMGWIIVFAWKPLAAAVDVAALRYLVAGGLAYT
                     IGAAFYLLKRRCWTHPVWHLFVGAGATLHFFAALALLPQG"
BASE COUNT          160 a          371 c          357 g          182 t
ORIGIN      
        1 gaacgacgcg ctggtctcgg ccgggatcgg cctcgtcata ggcaggataa cgtcggcgct
       61 cggcggcatg aagctgtcgg ttttcttctc caatattgtc ggcggagcct tcgcggccgc
      121 cgtcgccatc ggcgccgagg ccctccgcgt cgcgggcaat accgacgcga tgatcatcgg
      181 ctccatcatg ctactcgtgc ccggcgtgct catagtcaat gccatacggg acttgatagc
      241 cggcgacctg gtggctggca tagcccgggc ggccgacgcg ttcatctcgg cggccggcat
      301 ctcgatcggc gtcggcttgg cgctgcagct ggccatgctg atctcaggac cgacgccgtg
      361 agcgcccagg ccgtccagct ggcctgggcc ttcctggcca ccctcggctt cggggtactg
      421 ttcagggccc cgctccgcga cctgccattc accgccctcg ggggtgccgt atcgtggggc
      481 gcctacctgg ccttcaaggc ggccaccggc tccgacaccc tatcgtactt cgccgcgagc
      541 gtcgcggtcg gcctctatgc ggaggccatg gcgtcctgcc ttaaacggcc ggccaccctg
      601 ttcatcctgt cggccatcat accgctcgtg cccggcgcgg gcatgtaccg caccatgctc
      661 gaggcggtca acggccgcgc cgccgctagc gcgagcgtcg gcttcgagac gctgacgctg
      721 gccggagcca tcgccggtgg cctggcggta gcgtcggcgg cgtcgaggat tctctggctc
      781 aagaagccga ggctacctcg ccgcccaggc tcgcgtcagc cctgaggtag cagcgccagc
      841 gccgcgaaga agtggagcgt cgcgccggca ccgacgaaca ggtgccatac cgggtgggtc
      901 cagcaccgtc ttttgagcag gtagaaggcg gcgccgatcg tataggccag gcctccggcg
      961 acgaggtacc gtagggccgc gacgtcgacg gcggcagcaa gcggcttcca cgcgaagacg
     1021 atgatccatc ccatcagcac gtaggcgaca gtggagacga acttcatctt
//
LOCUS       DAOOLN010000979         1066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_189648_length_1066_cov_4.245302, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000979 DAOOLN010000000
VERSION     DAOOLN010000979.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189648_length_1066_cov_4.245302"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PLW80_11340"
     CDS             complement(<1..502)
                     /locus_tag="PLW80_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HPB67152.1"
                     /translation="MNAYIREVLDGLKARAPWETEFLQAAEEVLESIEPLIEKNPKYK
                     AQRILERIVEPERTIMFRVPWMNDKGEYIVNRGYRVQFNSAIGPYKGGIRFHPKVTLS
                     TLKFLGFEQTFKNSLTSLPMGGGKGGADFDPNGKSDNEIMRFCQSFMTELQKYVGPDT
                     DVPAGDI"
     gene            complement(720..>1066)
                     /locus_tag="PLW80_11345"
     CDS             complement(720..>1066)
                     /locus_tag="PLW80_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67153.1"
                     /translation="LALVGLGRVDGVAVGDAFILVKRGAVAIEPDGGGLAWKDSDVVA
                     RVVASRVDDELFEGKLERVGFFDRVNPRDVALREPPKPKPEAAQDSGGEASAAKASYV
                     WSALFERVRSLY"
BASE COUNT          192 a          330 c          339 g          205 t
ORIGIN      
        1 cgatgtcgcc agcgggcacg tcggtatcgg ggccgacata cttctgaagc tcggtcatga
       61 agctctggca gaagcgcatg atctcgttgt cgctcttgcc gttcgggtcg aagtcggcgc
      121 cgcccttgcc gccgcccatc gggaggctcg tgaggctgtt cttgaaggtc tgctcgaagc
      181 cgaggaactt gagggtggag agcgtcacct tcgggtggaa gcggataccg cccttgtacg
      241 ggccgatggc gctgttgaac tgaacgcgat agccgcgatt gacgatgtac tcgcccttgt
      301 cgttcatcca gggcaccctg aacatgatgg tgcgctcggg ctcgacgatg cgctcgagga
      361 tgcgctgggc cttgtacttg gggttcttct cgataagcgg ctcgatcgac tcgaggactt
      421 cctcggcggc ctgaaggaac tcggtctccc acggcgcgcg ggctttgagg ccatcgagga
      481 cctcgcggat ataagcgttc attcgccctc ctttaataga gctgacagac acgatgctcg
      541 gtacggacac tgatccgtcc atggaacatt gtacacattc agcggagtct atgcaagacg
      601 tttttcgatt attgatagcg gtatttcata tatcaaaatc actatcgaag gtacaccacg
      661 accgggcaag cgccgcgagt cgtggcagat gggtacaacg ctacggagga gggcgggcgt
      721 tagtagaggc tacgcacccg ctcgaacagg gcgctccaga cgtacgacgc cttggcggcc
      781 gaggcctctc cgcctgagtc ctgagcggcc tccggcttcg gctttggcgg ctctcgcagc
      841 gccacatcgc gcgggttgac gcggtcgaag aaccccaccc gctcgagctt accctcgaac
      901 agctcgtcgt cgacccgcga ggcgaccacg cgcgccacga cgtccgagtc cttccaggcc
      961 aagccgccgc cgtcgggctc gatggctacg gctccgcgct tgacgaggat gaaggcgtcg
     1021 ccgacggcca cgccgtcgac ccggcccagc ccgacgaggg ccaggt
//
LOCUS       DAOOLN010000980         1066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_189711_length_1066_cov_2.777448, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000980 DAOOLN010000000
VERSION     DAOOLN010000980.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189711_length_1066_cov_2.777448"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="PLW80_11350"
     CDS             complement(<1..1054)
                     /locus_tag="PLW80_11350"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPB67154.1"
                     /translation="MAIYAYEPGSVFKVDSMASLMALGGIDERSRFFCDGSYEKVTAS
                     GEEIVIKCLGHHGNVDVTNILEYSCNAGAAYASETVSDIDFYAKIREFGFGERVGADL
                     TGESPGLMRRPGDWSGRTKPTMAMGQELLVTALQMADAATVVANGGVLMRARTLGRIV
                     SADGIVLEEPQPIAVRRVMGEYEAKAILRSMEAAVLDSGTGRRARIDDLRMSVKTGTA
                     QVIDPRTRRYSDTDFIASTIALFPSEDPEYIVYAAIFNPKGASTYGGRIAAPFVKDAA
                     NAIADLYGVARSGSASATHGGRIAVSGVEAAEVGDAMPDLRGLPKRALTGLLARDDLV
                     VEIEGDGWVREQSPAPG"
BASE COUNT          171 a          372 c          335 g          188 t
ORIGIN      
        1 ccccgggcgc cggcgactgc tcgcgaaccc atccgtcgcc ctctatctcg acgacgaggt
       61 catcccgggc gagcagtccg gtcagcgccc gcttgggcag gcctcgcaaa tcgggcatcg
      121 cgtcgcctac ctcagccgcc tcgactccgc tgaccgctat ccgaccgcca tgggtcgcgg
      181 aggccgaacc ggagcgggcc acgccgtaga ggtcggcgat cgcgttcgcc gcgtccttga
      241 cgaagggggc ggcaatacgt ccgccgtacg tcgaggcgcc tttcgggttg aagatggcgg
      301 catacacgat gtactcgggg tcctcgctcg ggaagagcgc tattgtgctc gcgatgaagt
      361 cagtatccga atagcggcgc gtcctcgggt cgatgacctg ggccgtaccg gtcttcaccg
      421 acatgcggag gtcgtcgatg cgggcgcggc ggccggtacc ggagtcgagc acggccgcct
      481 ccatcgagcg cagtatcgcc ttcgcctcgt actcgcccat aaccctacgt accgctatcg
      541 gctgcggctc ctcgaggacg ataccgtctg ccgatacgat gcgaccgagc gtcctagcgc
      601 gcatcagcac gccaccgttc gccacgacag tcgccgcgtc ggccatctgc agcgccgtga
      661 cgagcagctc ctgtcccatc gccatggtcg gcttcgtcct gccggaccag tcccccggcc
      721 gcctcatgag cccggggctc tcgcctgtca ggtcggcgcc gacgcgctca ccgaaaccga
      781 attcccgtat cttggcatag aagtctatat ccgataccgt ctcggaggcg taggcggcgc
      841 cggcgttgca cgagtactcg agaatgttag tcacgtcgac gttgccgtgg tggccgaggc
      901 acttgatgac gatctcctcg ccggatgccg tcaccttctc gtagcttccg tcgcagaaga
      961 aacgcgagcg ctcgtcgatg ccgccgaggg ccatcaacga ggccatgctg tctaccttga
     1021 agaccgatcc cggctcgtag gcgtagatgg ccacgcggtc ggtcca
//
LOCUS       DAOOLN010000981         1065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_189988_length_1065_cov_2.833663, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000981 DAOOLN010000000
VERSION     DAOOLN010000981.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189988_length_1065_cov_2.833663"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PLW80_11355"
     CDS             complement(<1..201)
                     /locus_tag="PLW80_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67155.1"
                     /translation="MLMQAEFQDRCRARGLDEAAIERALETVRTFERSVPRGSSLPGL
                     RLAELDAAIASMIGQLDEGALMA"
     gene            complement(243..>1065)
                     /locus_tag="PLW80_11360"
     CDS             complement(243..>1065)
                     /locus_tag="PLW80_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HPB67156.1"
                     /translation="AVAGSGLPLDRRSAAAALGFARPSRNAMDATGDRDACVEYAAAA
                     STLLMHLSRFCEEIILWSSAEFGFMDIADDCSTGSSIMPQKRNPDVAELIRGKAGRAF
                     GSLVALLTIQKGLPLGYDRDLQEDKAALFDLRDTLAGVLPAFTALVEALKPRPAAMAA
                     ALEIGFAEATDLAELLVAKGVPFREGYAATKAAVAAAAAEGVGLAGLSDADLAAASGA
                     FAEAGIRASDLTAYLRPEACVGRRDGVGGPAPDRTREEIARLRAFIALNSACAPA"
BASE COUNT          163 a          373 c          364 g          165 t
ORIGIN      
        1 ggccatgagc gcgccctcgt cgagctggcc gatcatactc gcgatggccg cgtcgagctc
       61 agccagcctt agcccaggca ggctcgatcc gcgcggaacg gagcgttcga aggtcctgac
      121 ggtctcgagc gcccgttcta tcgccgcctc atccaacccg cgcgccctac accgatcctg
      181 gaattcggct tgcatcagca tcgcgtcccc ctcggctagg cgatagctga agtataggcg
      241 atctaggccg gcgcgcaagc cgaattcagg gctatgaagg cgcgcaatcg ggcgatctcc
      301 tcgcgcgtac ggtcgggcgc cggtccgccg acgccgtccc gacggccgac gcaggcctcg
      361 ggccgcaggt acgcggtcag gtccgaggcc ctgatcccgg cttcggcgaa ggcgccggac
      421 gctgccgcga ggtcggcgtc tgagagcccc gctaaaccga ctccttcggc ggccgccgcg
      481 gcgaccgctg ccttggtcgc cgcgtagcct tccctgaacg gtacgccctt cgcgacgagg
      541 agctcggcga ggtcggtagc ctcggcgaag ccgatctcga gggcggcggc catcgccgcg
      601 ggcctgggtt tcagagcctc gacgagggcg gtgaacgccg gcagaacgcc ggccagggta
      661 tcgcgtaggt cgaatagcgc ggccttgtcc tcctggaggt cgcggtcgta gccgagaggc
      721 aggcctttct gaatcgtgag cagcgcgaca agggagccga aggccctccc ggccttgcct
      781 cgtatcaact cggcgacgtc cgggttgcgc ttctgcggca tgatgctcga ccccgtcgag
      841 caatcgtcgg ctatgtccat gaagccgaac tcggcgctcg accacaggat gatctcctcg
      901 cagaagcgcg acaagtgcat gagcagagtc gacgccgcgg ccgcgtactc cacgcaggcg
      961 tctcggtctc cggttgcgtc catcgcgttg cggctcggtc gcgcgaagcc cagagcggcg
     1021 gccgccgacc tgcggtcgag cggcagtccc gagccggcta cggcc
//
LOCUS       DAOOLN010000982         1062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_190853_length_1062_cov_3.579940, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000982 DAOOLN010000000
VERSION     DAOOLN010000982.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_190853_length_1062_cov_3.579940"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="PLW80_11365"
     CDS             complement(<1..942)
                     /locus_tag="PLW80_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB67157.1"
                     /translation="MKRAVVLSALLALVVSFAAAQQSVSAYTTLEEPLAKALFEKFEA
                     ETGIKVNFVRLSGGEVVTRLEAERANPQASIWVGGVGLDHITAKDKGLTTPYKSRAAG
                     KTPAEFKDKDGYWVGMYVGPLTFVTNTVRAAELGIKPPQSWADLLKPEYKGMIRVANP
                     NTSGTAYNVITTLRYIYKGDEQKVYDYLKKLDANIDQYTRSGSAPGKSVAIGEIPIAI
                     GYAHDQVKLKAGGAPVVITAPKEGTGYELASMSLVAGGKDAVNAKKLYDWVLSSPTAQ
                     AEFVNYYLVLVTPGAAKHPDALSLKEIGGEAPVGWGIA"
BASE COUNT          162 a          310 c          358 g          232 t
ORIGIN      
        1 cgcgatgccc cagcccaccg gggcctcccc gccgatctcc ttgagcgaca gggcgtccgg
       61 gtgcttggcg gcgcctggcg tcacgaggac caggtaatag ttgacgaact cggcctgggc
      121 ggtcgggctg gataggaccc agtcgtagag cttcttggcg ttgaccgcgt ccttgcctcc
      181 ggcgaccagg gacatcgacg ccagctcgta gccggtgccc tccttggggg ctgtgatcac
      241 gacgggcgcg ccgccggcct tgagcttgac ctggtcgtgt gcgtagccta tggctatcgg
      301 gatctcgccg atcgcgacgc tcttgccggg ggccgagccg gagcgggtgt actggtcgat
      361 gttggcgtca agcttcttga ggtaatcgta aaccttctgc tcgtcgccct tgtagatgta
      421 gcggagcgtg gtgatcacgt tataggccgt gcccgaggtg ttcgggttgg ctacgcgtat
      481 catgcccttg tactcgggct tcaggaggtc tgcccagctc tgcggcggct tgatgccgag
      541 ctcagcggcg cggacggtat tggtcacgaa ggtgagcggc cccacgtaca tgccgaccca
      601 gtagccgtcc ttgtccttga attcagccgg ggtcttgccg gcggccctgg acttgtaggg
      661 cgtggtcaga cccttgtcct tggccgtgat gtgatcgagg ccgacgcccc cgacccagat
      721 cgaggcctgg gggttggccc gctcggcttc gaggcgggtg actacctcgc cgccggacag
      781 gcgtacgaag ttgaccttga tcccggtctc ggcctcgaat ttctcgaaca gggccttggc
      841 gagcggctcc tcgagcgtcg tataggcgct gaccgattgt tgggccgcgg cgaacgatac
      901 gacgagcgcc aggagcgctg atagtactac agcacgcttc atgattcctc cgtgcgcgga
      961 cattactgtc gcgcggtgat tttctataat tgtacgcgca tcttggtagg gcgcgcaaat
     1021 taaatatggt tgaagaaagc ggattctagt tggatttttc gg
//
LOCUS       DAOOLN010000983         1061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_191106_length_1061_cov_4.266402, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000983 DAOOLN010000000
VERSION     DAOOLN010000983.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191106_length_1061_cov_4.266402"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1061)
                     /locus_tag="PLW80_11370"
     CDS             complement(<1..>1061)
                     /locus_tag="PLW80_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HPB67158.1"
                     /translation="STTLVIESGHPLGLFKSHELAPRVVITNGLMIGMFDNQKDFNHA
                     AALGVANYGQMTAGGWMYIGPQGIVHGTFNTLLNAGRLKLGVARDGDLRGKLFVSSGL
                     GGMSGAQGKAAVIAGASSIIAEVDESRVVTRHSQGWVDRVEKTVAGAFARAEEARSKG
                     EPIAVAYLGNIVDLLEYADSHGVRIDLLSDQTSCHAAYEGGYCPAGVSFEERTRLLAE
                     DPARFRSLVDESLRRHFDVIGRLTGKGTYFFDYGNSFLKAVYDAGVREVSKNGVDEKD
                     GFVFPSYVEDILGPELFDYGYGPFRWVCLSGKDEDLRKTDAAAMACIDPNRRGQDRDN
                     WAWIRDAEKNRLVVGTKAR"
BASE COUNT          163 a          358 c          357 g          183 t
ORIGIN      
        1 tacgcgcctt cgtgccgacg acgagcctat tcttctccgc gtcccgtatc caggcccagt
       61 tatcgcggtc ctgcccgcgt cggttcgggt cgatgcaggc catcgcggcg gcgtcggtct
      121 tgcgcaggtc ctcgtccttg ccggacaggc agacccagcg gaacggcccg tagccgtagt
      181 cgaacagttc cggccccagg atgtcctcga cgtagctcgg gaagacgaag ccgtccttct
      241 cgtcgacgcc gttcttggag acctcgcgca cgccggcgtc gtagaccgcc ttgaggaacg
      301 agtttccgta gtcgaagaag taggtaccct tgccggtcaa gcgcccgatc acgtcgaagt
      361 gacggcgcag gctctcgtcg acgagggagc ggaaccgggc cggatcctcc gccagcaggc
      421 gggtgcgctc ctcgaacgag acgcccgcgg ggcagtaccc gccctcgtac gcggcgtggc
      481 agctcgtctg gtcggacagg aggtcgatcc gcacgccgtg ggagtcggcg tactcgagca
      541 ggtcgacgat attgcctagg taggcgaccg ctatcggctc ccccttcgaa cgggcctcct
      601 ccgcccgggc gaaggcgccg gccaccgttt tctcgacgcg gtcgacccag ccctgggaat
      661 gcctcgtgac gacgcggctc tcgtcgacct cggcgattat ggagctggcc ccggctatga
      721 cggcggcctt gccctgggcg ccggacatgc cgccaaggcc ggacgagacg aacagcttgc
      781 cgcggaggtc gccgtcacgc gcgaccccca gcttcaggcg gccggcgttg agcagcgtat
      841 tgaaggtacc gtggacgatg ccctgcgggc ctatgtacat ccagccgccg gcggtcatct
      901 ggccgtagtt ggccacgccg agggccgccg cgtggttgaa gtccttctga ttgtcgaaca
      961 tgcctatcat caggccgttc gtgattacga cgcgcggcgc cagctcgtgg ctcttgaaca
     1021 ggccgagcgg atggccggac tcgatgacga gggtcgtcga c
//
LOCUS       DAOOLN010000984         1059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_191746_length_1059_cov_3.235060, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000984 DAOOLN010000000
VERSION     DAOOLN010000984.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191746_length_1059_cov_3.235060"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PLW80_11375"
     CDS             complement(<1..637)
                     /locus_tag="PLW80_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPB67159.1"
                     /translation="MDNPLQLSFVNFKRDSYIIVEGKQNADRFFIIKSGKVRISKEVE
                     VVEEEGGNIYSPGDFFGVISTMSSHSHIETAQAITDVTLISVHRDQYGLLIEKNTPVA
                     MKIIMGFSKRMRYLDEALTRITLKKTAEIDPSHLYKVAECYARQSQFNQAYYAYYQYL
                     KFCPNGQHASSAKERMAKIKPYSKAVYLDGSVEEFNRYYPKDTMVFSEGMPG"
     gene            complement(767..>1059)
                     /locus_tag="PLW80_11380"
     CDS             complement(767..>1059)
                     /locus_tag="PLW80_11380"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456497.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPB67160.1"
                     /translation="DLSPLGITGKEAEKWLDEAGITVNKNGIPFDQKSPFITSGIRVG
                     TPAVTTRGMKEPEMRQIASLIMEVLRTKGESADKVRSEVRKLTGRFGLPADL"
BASE COUNT          170 a          311 c          341 g          237 t
ORIGIN      
        1 tgccgggcat gccctccgag aacaccatcg tgtccttcgg gtagtagcgg ttgaattcct
       61 cgaccgagcc gtcgaggtag acggccttgg agtacggctt tatcttggcc atccgctcct
      121 tggccgacga ggcgtgctgc ccgttcgggc agaacttgag gtactggtag tacgcgtagt
      181 aggcctggtt gaactggctc tgcctggcgt agcactcagc gaccttgtac aggtgggacg
      241 ggtcgatctc ggcggtcttc ttgagggtta tgcgggtcag cgcctcgtcg aggtagcgca
      301 tgcgcttcga gaagcccatg atgatcttca tcgcgaccgg ggtattcttc tcgatcagga
      361 ggccgtactg gtcgcgatgc acgctgatga gcgtcacgtc ggtgatggcc tgggccgtct
      421 cgatatggct atggctggac atggtggaga tgacgccgaa gaagtcgccg ggcgagtaga
      481 tgttgccgcc ctcttcctcg acgacctcca cttccttgga gatccgcacc ttgccggact
      541 tgattatgaa gaagcggtcg gcgttctgct tgccctcgac gatgatatac gagtctcttt
      601 tgaaattgac gaaggacagc tggagcggat tatccatgcc gttaccgttt ctcccgtcgc
      661 gaagctctct cgagtatagg tacgccccgg ggggttgtca attgccgata gtccggcgcc
      721 ccggggcgcc cggggaacat ccccgggatc gctacggcct agtgtatcac agatcggccg
      781 gaaggccgaa gcgtccggtc agcttccgca cctcggagcg gaccttgtcg gccgactcgc
      841 ccttcgtgcg cagcacctcc atgatcagcg aggcgatctg acgcatctcc ggctccttca
      901 tgccgcgggt cgtgaccgcc ggcgtgccga ctcgtatgcc cgaggtgatg aaggggctct
      961 tctggtcgaa cgggatgccg ttcttgttga cggtgatgcc ggcctcgtcg agccacttct
     1021 ccgcctcctt gccggttatg ccgaggggcg acaggtcga
//
LOCUS       DAOOLN010000985         1056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_192731_length_1056_cov_2.596404, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000985 DAOOLN010000000
VERSION     DAOOLN010000985.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192731_length_1056_cov_2.596404"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PLW80_11385"
     CDS             <1..428
                     /locus_tag="PLW80_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPB67161.1"
                     /translation="IGVAKTTEILLCMLEANEARGRRRYVRAACPPDSTELSCPTDEG
                     LLRGWIKDLSSVGMSVAFAGGATPRQGSRLRDMQLNLKGVRVILNGVVIGSHDAPGIG
                     TISVIMFEPASVNDDKRAKLRAFIRRTLQATMDKALSLA"
     gene            complement(435..>1056)
                     /locus_tag="PLW80_11390"
     CDS             complement(435..>1056)
                     /locus_tag="PLW80_11390"
                     /inference="COORDINATES: protein motif:HMM:NF015817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HPB67162.1"
                     /translation="GAAEDGAAEDGAAPACEPTVEPGAAKPKRKAKAKAKADPAVASF
                     YTLADELLAMLPPREALASLAAAAYSGDLDPRRYGEVRDVSVDSAATAHLYVGIGKQD
                     GAWPKDVAAVVKRLTGLPDRLVDDIEVLERFSFVSVPFEAAEKAIAEARKQRGAPPVR
                     MATPKSGPDGPGGASGGKRGYGPRPSYGSKGGYDRPKPYKKPRPRD"
BASE COUNT          144 a          373 c          380 g          159 t
ORIGIN      
        1 aaatcggcgt ggccaagacc accgagatac tcctgtgcat gctcgaggcc aacgaggcgc
       61 gcggccgcag gcgctacgtg cgcgcggcct gccccccgga ctcgaccgag ctgagctgcc
      121 ccaccgacga gggcctgctg cgcggctgga tcaaggacct gagttccgtc ggcatgtcgg
      181 tcgccttcgc cggcggggcc acgccgaggc agggctccag gctcagggac atgcagctga
      241 acctcaaggg cgtgcgcgtg atcctgaacg gcgtcgtgat cggcagccac gacgcgccgg
      301 gaataggcac gatatcggtg atcatgttcg aacccgcgag cgtcaacgac gacaagcgcg
      361 ccaagctgcg cgccttcatc aggcggacgc tccaggcgac gatggacaag gccctgtcgc
      421 tggcctgagc cgggctagtc gcgcgggcga ggcttcttgt acggcttggg tcggtcgtag
      481 ccgcccttcg agccgtacga cggcctcggg ccgtagcccc gcttgccccc ggacgctccg
      541 cccgggccgt ccgggccgct cttgggcgtg gccatccgca cgggcggcgc gccgcgctgc
      601 ttcctcgcct cggctatcgc cttctccgcg gcctcgaagg gcacgctgac gaacgagaag
      661 cgctcgagca cctcgatatc gtcgaccaag cggtcaggta gcccggtcag gcgcttgacg
      721 acggcggcca cgtccttggg ccaggcgccg tcctgcttgc cgatacccac gtacaggtgg
      781 gccgtcgcgg ccgagtcgac cgacacgtcg cggacctcgc cgtagcgcct cgggtcgagg
      841 tcgccggagt aggccgccgc ggcgagcgag gcgagcgcct ccctgggcgg caacatcgcg
      901 agcagctcgt cggccagcgt atagaacgag gcgacggctg ggtcggcctt ggccttggcc
      961 ttggccttgc gtttgggctt ggcggcgcct ggctcgacgg tcggttcgca ggcgggggcg
     1021 gctccgtcct cggcggctcc gtcctcggcg gctccg
//
LOCUS       DAOOLN010000986         1056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_192813_length_1056_cov_2.131868, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000986 DAOOLN010000000
VERSION     DAOOLN010000986.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192813_length_1056_cov_2.131868"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PLW80_11395"
     CDS             complement(<1..606)
                     /locus_tag="PLW80_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67163.1"
                     /translation="MSMAMSDAVIERIADRIAAERGDGCRERALEGVRCAAPLWRDED
                     GTQTDLEAFCLRQFCATDAERDSLFLRLESVLEELYGSALALSRTFSWGVHVDRGPVL
                     PVDAILAEWSPYAHFSDDFYGNKLAFVIHLNFPYKDLDRKLRDGPSWTRHQWAEARLG
                     DQFLDRVPSEASQRLAAAAAEAETYIAGYYLYPSRLRDASGA"
     gene            740..>1056
                     /locus_tag="PLW80_11400"
     CDS             740..>1056
                     /locus_tag="PLW80_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67164.1"
                     /translation="MPRTEKTRIYLLACVIASMTLLGATPEPPFEPLEHIPAGAVAVA
                     SVGDLTSLLSNSIGFMRNAGLGSEAAELSTLIDGALHPEGADSTDAAALFKAIDPGRR
                     VVAA"
BASE COUNT          189 a          362 c          335 g          170 t
ORIGIN      
        1 ggctccggac gcgtccctga gcctcgacgg gtacaggtag tagccggcga tataggtctc
       61 ggcctcggcc gcagcggcgg cgagccgctg cgaagcctcc gacggcacgc ggtccaagaa
      121 ctggtctccg agcctggcct cggcccattg atgccgggtc cacgaaggtc catcgcgcag
      181 cttacggtcg agatccttgt atgggaagtt caggtgaatg acgaaggcga gcttgttgcc
      241 gtagaagtcg tcgctgaaat gggcgtaggg cgaccattcc gcaaggatcg cgtccaccgg
      301 caggaccggc ccccggtcga cgtggacgcc ccaggagaag gtcctggaca gggcgagggc
      361 cgagccgtac agctcctcga ggaccgactc gagcctcagg aacaacgagt cgcgctcggc
      421 gtccgtagcg cagaactgcc gcaggcagaa cgcctcgagg tcggtctgcg tgccgtcctc
      481 atcgcgccag aggggcgccg cgcagcgtac gccctcgagc gcccgctccc tgcaaccatc
      541 gcctcgttcg gccgcaatcc tgtccgctat acgctcaatg accgcgtcgc tcatcgccat
      601 actcatcgtc gacctcctgg caccggccct atcctatacc ggaggcggcc agcgggtaag
      661 cgacggcccg ctggatactt cgggtccggt acggtattct tttcttaatt aatgatcgat
      721 agcacggagg aacgccagca tgccccgcac tgagaaaacg cgcatctacc tactagcctg
      781 cgtcatcgcc tcgatgacgc ttttaggcgc cacacccgag ccgccgttcg aaccgctcga
      841 gcacatcccc gcgggggccg tcgcggtggc cagcgtcggc gacctgacgt cgctcctgag
      901 caactcgata ggcttcatgc ggaacgccgg tctcgggagc gaagccgccg agctatcgac
      961 gctcatagac ggcgcgctgc atccagaagg cgccgattcc acggacgcgg ccgccctgtt
     1021 caaagcgatc gaccccggca ggcgcgtcgt ggcggc
//
LOCUS       DAOOLN010000987         1055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_193016_length_1055_cov_3.019000, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000987 DAOOLN010000000
VERSION     DAOOLN010000987.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193016_length_1055_cov_3.019000"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLW80_11405"
     CDS             <1..180
                     /locus_tag="PLW80_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67165.1"
                     /translation="VQALAFAVVLAQIMGGSEVRLDAYRVHDGSSSTIIPSAGSAFKR
                     SSPESDPGKFTAWSP"
     gene            232..>1055
                     /locus_tag="PLW80_11410"
     CDS             232..>1055
                     /locus_tag="PLW80_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPB67166.1"
                     /translation="MSKRLIELVPAGQPAELTGNPDVAIEGLAYDSREVGPGYLFFAF
                     PGLHADGHDYVAKAISAGAAAVVLERMPPSPAPGVSYLRVPDARAAMSPIADAFFGRP
                     SRRLKVIGVTGTEGKSTTVWLIYRLLDLAGKKAGFFSTVEYRVADRVEANPKHQTTPE
                     APTVHEHLARMAASGFEYAVVEASSHGLSPRTGRLADLAFDVGVMTNVRHEHLEFHGT
                     WERYRDDKMRLFQGLDSVDHRKSVLGVIVDVPSFGVVCADDPSAGRFAASTGKPVYR"
BASE COUNT          171 a          363 c          343 g          178 t
ORIGIN      
        1 gtgcaggcgc tcgcatttgc cgtggtactc gctcagataa tgggcggcag cgaagtccgc
       61 ctcgacgcgt atcgtgtaca tgatggctcc agttccacaa taataccgag cgccggttca
      121 gccttcaagc gttcgtcacc cgagtccgat cctggcaaat ttacggcatg gtcgccgtaa
      181 acgaggctcg atatattgcg caagccaagc ggcgtcgcta ggatcgtggg catgagcaaa
      241 cgattgatcg agctcgtccc ggctggccag cccgccgagt tgacggggaa tcccgacgtc
      301 gccattgagg gcctcgcgta cgactcgcgg gaggtcggac ccggctacct cttcttcgcg
      361 ttccccggcc tccacgccga cggccacgac tacgtcgcta aggcgatctc ggccggcgcc
      421 gccgcggtcg tcctggagcg catgccgccg tcgccggcgc ctggcgtgtc atacctacgg
      481 gtgcccgacg cgagggccgc catgtcacct atcgccgacg cattcttcgg ccgcccgtcg
      541 cggcggctca aggtgatcgg agtgaccggt accgagggca agagtacgac ggtctggctg
      601 atctaccgac tcctggacct ggccgggaag aaggccgggt tcttctccac agtggagtac
      661 cgcgtggccg accgggtcga ggccaacccc aagcaccaga ctacgcccga ggccccgacc
      721 gtccacgagc acctggcgcg catggccgcg tcgggcttcg agtacgccgt cgtcgaggcc
      781 tcgtcgcacg gcctgtcgcc gcgaaccggg cgcctcgcgg acctggcctt cgacgtcggc
      841 gtgatgacga acgtgcgcca cgagcacttg gagttccacg gcacctggga acgctaccgc
      901 gacgacaaga tgcggctgtt ccagggcctc gactcggtcg accaccgcaa gtccgtgctt
      961 ggcgtcatcg ttgacgtgcc gagcttcggc gtcgtgtgcg ccgacgaccc gagcgccggg
     1021 cgcttcgcgg cgtcgaccgg aaagcctgtc taccg
//
LOCUS       DAOOLN010000988         1055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_193138_length_1055_cov_2.051000, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000988 DAOOLN010000000
VERSION     DAOOLN010000988.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193138_length_1055_cov_2.051000"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..>1055)
                     /locus_tag="PLW80_11415"
     CDS             complement(152..>1055)
                     /locus_tag="PLW80_11415"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB67167.1"
                     /translation="SLGVDERYAVIQKISSSLSDAEDYARLVDFLSEVVARDPEGQYN
                     ARHLLTIAWAYSRQGADDIAELYYDRVLKNFPDLTVNGESIHFACLRRLIELSENSAR
                     RIEFRRELIARFPEKIDMGIALFLLGYDYESIGDWDAALDSYRRFLPASGAEVPGHPD
                     AIQYAQQFIDLATIRKDWTYETLDELLEAVSTAMATGNARSLARIRAKVGFFAMDWYQ
                     DKSDGNSQVLFDFAAFTSGTRIQRAPALDPSSNANEAFLRTWGWTERISVWYLYFRKV
                     NFPADPEVHGRWEWAGIYFGEKMQ"
BASE COUNT          182 a          323 c          361 g          189 t
ORIGIN      
        1 cgggtggaaa acggcgcaga gcggcaactg gtgatggccg ccgccgccgg ggaaggggtc
       61 ggcgtcccgg cctgaccgtc taggccgaag gcccgccccg cgagcgcggc ggtcgccagg
      121 gtcgcggcga cggccgcgac ggccgggcgt ctcattgcat cttctccccg aagtagatgc
      181 ctgcccactc ccagcggccg tgcacctccg ggtcggcggg aaaattcact ttgcggaagt
      241 acaggtacca gaccgagata cgctcggtcc atccccaggt gcgtaggaag gcctcgttgg
      301 cgttcgagct cgggtcgagg gctggagccc gctggatgcg cgtaccggag gtgaaagccg
      361 cgaaatcgaa cagcacctgg ctgttgccgt cggacttatc ctggtaccag tccatagcga
      421 agaagccgac cttggcgcgt atcctggcga gcgatcgcgc gttgcccgtc gccatggccg
      481 tcgagacggc ctcgagcagc tcgtcgagtg tctcgtaggt ccagtccttg cgtatggtcg
      541 ccaggtcgat gaactgctgg gcgtactgga tcgcgtcggg gtggccgggt acctctgcgc
      601 ccgacgcggg aaggaagcgt cggtacgagt cgagggccgc gtcccagtct ccgatggact
      661 cgtagtcata gccgagcagg aacagcgcta tacccatatc tatcttttcg ggaaatctgg
      721 cgatgagctc gcgcctgaat tcgatgcgcc tcgccgaatt ctccgataat tctatcaggc
      781 ggcgcaggca ggcgaagtgg atggactccc cgttgaccgt caggtcggga aaattcttga
      841 gtacccggtc gtagtacagc tccgcgatat cgtcggcgcc ctgcctcgag taggcccagg
      901 ctatggtcag gagatgcctg gcgttgtact ggccctcggg gtcgcgcgcg accacctcgg
      961 acaggaagtc gacgagccgc gcgtaatcct cggcgtcgga cagcgacgag gatatcttct
     1021 ggatgacagc gtagcgctcg tcgacgccga ggctt
//
LOCUS       DAOOLN010000989         1054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_193320_length_1054_cov_2.810811, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000989 DAOOLN010000000
VERSION     DAOOLN010000989.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193320_length_1054_cov_2.810811"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PLW80_11420"
     CDS             complement(<1..471)
                     /locus_tag="PLW80_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67168.1"
                     /translation="MKRIFVAAAALCALCGLASAQDADSADDYVLQKAREEYAASSGS
                     GDQAATDQDAVMEQSTVIAVPQAPATKTVTTDGDGQSTVITIHIAEDKPSAETDDDER
                     RRRYTPAVIGFVPGLSLPFGVYDTSLSLASVGALTGDVYGFQGSGVFNIADDVGI"
     gene            637..>1054
                     /locus_tag="PLW80_11425"
     CDS             637..>1054
                     /locus_tag="PLW80_11425"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPB67169.1"
                     /translation="MNVNALPRLTIANLLAGELDRGVAEGVFRCGVVALTDGGETVMA
                     ACGQARRILDPSGSWDAVAADNRSVFDLASLTKIVATLPVSLLTIQSGRMALDDPASR
                     WLPELAAGAGGSWNGAITIRSLLGHFSGLPAWRPYFV"
BASE COUNT          174 a          323 c          342 g          215 t
ORIGIN      
        1 gatgccgacg tcgtcggcga tgttgaacac gccggagccc tggaagccat atacatcgcc
       61 ggtcagggcg ccgacggagg ccagcgacag cgaggtgtcg tagacgccga agggtagcga
      121 taggcccggt acgaagccta tcacggcggg cgtatagcgg cgccggcgct cgtcgtcgtc
      181 cgtttcagct gagggcttgt cctcggcgat atggatggtt atgacggtcg actggccgtc
      241 gccgtccgta gtgacggttt tggttgccgg ggcctgaggc accgcgatca ccgtggactg
      301 ctccatgacg gcgtcctggt cggtagccgc ctggtcgcct gagccagacg aggccgcata
      361 ctcttcgcgg gctttctgga ggacgtagtc atcggctgaa tccgcatcct gggcggacgc
      421 gaggccgcat agggcgcaca aggccgccgc ggctacaaag atacgtttca tggttctccc
      481 cactatggcg agtgtcggtt atgcctccgt acggagcgca tatgatgtac tataccgcga
      541 aggctacgga aatcaacgat cttattgctc ggcgaaaatt ccttttaata aaaattgttg
      601 ctcgatgaaa tattgtgtcg tatactcagc gggaccatga acgtcaacgc tctccctagg
      661 cttaccatcg ctaacctact cgctggcgag ctcgatcgcg gcgtcgcgga aggcgtgttc
      721 cgctgtggcg tcgtcgcgct gacggacggc ggcgagaccg tcatggccgc ctgcggtcag
      781 gccaggcgca tcctcgatcc gtcgggatcc tgggacgccg tcgcggcgga caatcgctcc
      841 gtcttcgacc tggcgtccct taccaagatc gtcgcgaccc tgcccgtcag cctgctgacg
      901 atccagtcgg gccggatggc cctggacgac ccggcctcgc gctggttgcc tgagctggcc
      961 gctggcgccg gcggctcatg gaacggcgct atcaccatac gctcgctgct cgggcatttt
     1021 tctggccttc cggcgtggag gccgtatttc gtgc
//
LOCUS       DAOOLN010000990         1054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_193396_length_1054_cov_2.211211, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000990 DAOOLN010000000
VERSION     DAOOLN010000990.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193396_length_1054_cov_2.211211"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..565)
                     /locus_tag="PLW80_11430"
     CDS             complement(98..565)
                     /locus_tag="PLW80_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67170.1"
                     /translation="MSQNNQVSLAIPEEDMKKIDAAIETLHDKLMPHLKTLGPNDRRK
                     MLKLGDKSVAFVQKSYDYGKQYKDLGPRFIQMDAFGTDIKASSTLQLMINRLEPLLVA
                     LEDSLTLSGSEAYQNALLFYQSIKGAAKAKDPNAKMILDHLSDQFKRAPRSKD"
BASE COUNT          184 a          287 c          342 g          241 t
ORIGIN      
        1 gcgaggggtt cggaaggtct cgcgaggggt tcggaaggtc tcgcgagggc agggggccgg
       61 cgccgaagct ctccatggcg cgccgggcgg tcgttcgtca gtccttgctg cgcggggccc
      121 gcttgaactg gtcggagagg tggtcgagga tcatcttggc gttgggatcc ttggccttgg
      181 ccgcgccctt gatgctctgg tagaagagca gggcgttctg gtaggcttcg gagccggata
      241 gggtcaggct atcctcgagg gcgaccagca agggctcgag ccggttgatc atgagctgga
      301 gcgtgctcga ggccttgatg tcggtgccga acgcgtccat ttggatgaag cgcggcccga
      361 gatccttgta ctgcttgccg taatcgtatg acttctgaac gaaggcgacc gacttgtcgc
      421 cgagcttgag catcttgcgc cggtcgttcg gcccgagcgt ctttaagtgg ggcatcagct
      481 tgtcatgcag cgtctcgata gccgcgtcga tttttttcat atcctcctcc gggatagcca
      541 agcttacctg attgttctgg gacataccgc gctcctcttt aaaaggttta gtaagtcctt
      601 gtttggaagc catggaatct tccaggtggg ggctacgact gaagaatcga tcacgaagta
      661 gcgaatagca agggaaatgg ccgaaaaaag aaattagacc cgatcggggc gtcgaagctc
      721 aagctccggg cgaaacacga cacgccgccc tgatgctcgg gatcttatac aggcctcgta
      781 tagcatctcg gagggtctgc ccaagagtcg ggaccctcgc tctcgcgagg gccgctgtct
      841 cttggtcacc ctcctccccg tgcgtgcgag gcctggttac gaacagcctt ctcggggcgg
      901 cgcgtcgtgc cgggtatcgg gctgtgctca gtatctggtt tgcgattcgg tcgaagcaat
      961 catagcgtcc gatagcctgg tactatcttg tttatcctgt ctgcattcat tctagacgac
     1021 acccgcctcc ccgtgcgtgc gaggcctggg tacg
//
LOCUS       DAOOLN010000991         1053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_193578_length_1053_cov_3.191383, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000991 DAOOLN010000000
VERSION     DAOOLN010000991.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193578_length_1053_cov_3.191383"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PLW80_11435"
     CDS             complement(<1..445)
                     /locus_tag="PLW80_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HPB67171.1"
                     /translation="MGIQAFFNAFTRWARPALDIAILAYLLYAMYRLLIKTQAVQLAK
                     GAALLVFVYAGAFFLKLETLSWVLNLLAPGLVIAMAIIFQPELRKIFIKLGQGGIFKR
                     GQGPRSTQLDAILHAAELLAEKRRGALIAFVRFVALDDIVERGTRI"
     gene            complement(473..>1053)
                     /gene="folP"
                     /locus_tag="PLW80_11440"
     CDS             complement(473..>1053)
                     /gene="folP"
                     /locus_tag="PLW80_11440"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPB67172.1"
                     /translation="SVDTRKASVARAALDAGADIVNDVAALLDDPGLAPLCAERGAPV
                     VLMHKKGVPERMQDGPWYDDCVSEVKAFLLAAADAAIASGVSRDAILLDPGVGFGKRL
                     DDNLALMARLDEIAASGYPVLVGLSRKSFIGALSGGGAEDRLPGSLAAACVARARGAV
                     VFRVHDVAETVQALAVFDAAMRGCASVRATLE"
BASE COUNT          180 a          355 c          366 g          152 t
ORIGIN      
        1 cgatcctggt gccgcgttcg actatgtcgt cgagggccac gaagcgcacg aaggctatca
       61 gcgccccgcg gcgcttctcg gcgagtagct ccgcggcgtg cagtatggcg tcgagctggg
      121 tcgagcgcgg cccctggccc cgcttgaaga tgccgccctg gccgagcttg atgaagatct
      181 tgcgcagctc cggctggaag atgatcgcca tcgctatgac gaggccgggg gccagcaggt
      241 tgagcaccca ggacagggtc tcgagcttca ggaagaaggc gccggcgtag acgaacacga
      301 gcagggccgc gcccttggcc agctgcaccg cctgcgtctt gatgagcagg cggtacatgg
      361 cgtacaggag gtacgcgagt atggctatgt cgagggccgg acgggcccat cgagtaaacg
      421 cgttgaagaa cgcctgtatg cccatcgtcc cgtccgtccg ccttccgtcg ttctattcca
      481 gagtggcccg taccgaggca cacccgcgca tggccgcgtc gaagacggcc agggcctgga
      541 ccgtctcggc cacgtcatgc accctgaaga cgactgcccc gcgcgccctg gcgacgcagg
      601 cggccgccag gctacccggg agcctatcct cggccccgcc cccgctgagc gcccctatga
      661 acgacttccg cgacaggccg acgaggacag gatagcccga ggcggctatt tcatcaagcc
      721 gagccatgag ggcgagatta tcgtcgagcc gtttgccgaa gccgacgcca gggtcgagga
      781 ggatggcgtc gcgcgagacc ccggacgcga tcgcagcgtc ggcggccgcg agcaggaagg
      841 ccttgacctc ggagacgcag tcgtcgtacc agggaccgtc ctgcatacgc tcgggcacgc
      901 ccttcttatg catcaagacg accggggcgc cgcgctcggc gcagagcggg gccagccccg
      961 ggtcgtcgag cagggcggcc acgtcgttga cgatgtcggc gccggcgtcg agagccgccc
     1021 gggccaccga ggccttgcgc gtgtccaccg aga
//
LOCUS       DAOOLN010000992         1053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_193629_length_1053_cov_2.573146, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000992 DAOOLN010000000
VERSION     DAOOLN010000992.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193629_length_1053_cov_2.573146"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1053)
                     /locus_tag="PLW80_11445"
     CDS             complement(<1..>1053)
                     /locus_tag="PLW80_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67173.1"
                     /translation="AHGNPLEPTVAEGYPKAAVTVAEDSVDVDADPITPDVVGWDITP
                     PAVAEALIGVGSGYFDVVPLASGSEIDSFELQFTEYVRDSSFYYANALDTTMPAGLPG
                     WSFKDADAEAVFRFGATGFDTQVTSTAFTGASVSLIDDALVRMDPVDPINPVWTARSQ
                     LEFRYAQGAALVTDLAGNLLPSYVPSLCAEKLPPKVRFVSAEVGTSRIYLQFTEPVWH
                     DGTRAFVSGDFSLSGTSATVSGLELITPSGPVSELWLDLSEPVTTAFALDARIALGAL
                     VMDRGSNYADPAVLRRAVDIAVGPVSVLGASDGVHVGDETTTEAALAAGALGLLRTFD
                     GSGRLYDMDTTIFTGLD"
BASE COUNT          179 a          339 c          372 g          163 t
ORIGIN      
        1 gtcgaggccc gtgaagatgg tcgtatccat atcatacagc cggcccgagc cgtcgaaggt
       61 ccgcaggagg ccgagcgcgc cggcggccag ggcggcttcg gtcgtcgtct cgtcgccgac
      121 gtgtacgccg tcggaggcgc cgaggaccga gaccgggccg acggcgatat cgacggcccg
      181 cctgagcacg gcggggtcgg cgtagttgct gccacggtcc atgaccagcg cgccgagcgc
      241 gatgcgcgcg tcgagcgcga aggccgtggt gaccggctcg gacagatcga gccaaagctc
      301 gctgacgggt ccgctcggcg tgatgagctc tagcccagaa accgtggccg aggtgccgct
      361 gagcgagaaa tcgccggaga cgaaggcgcg ggtaccgtcg tgccagaccg gttcggtgaa
      421 ctgtaggtag atccggctgg tgccgacctc ggcgctgacg aagcgcacct tgggcggcag
      481 cttctcggcg cacaggctcg gtacgtaact cggtagaaga ttaccggcca ggtcggtgac
      541 gagggccgct ccctgagcat acctgaactc aagctggctc ctcgccgtcc ataccgggtt
      601 gatgggatcc accggatcca tcctgacgag ggcgtcgtca atcagcgaga cggaagcgcc
      661 cgtgaaggcc gtgctcgtca cctgggtatc gaagccggtc gccccgaagc ggaatacggc
      721 ctcggcgtcc gcgtccttga acgaccagcc tggcaggccg gcgggcatgg tcgtatccag
      781 ggcgttcgcg tagtagaacg acgagtcgcg tacgtactcg gtgaactgca gctcgaagct
      841 gtcgatttcc gacccgctcg ctagcggcac cacgtcgaag tagccggacc cgacgcctat
      901 cagcgcctcc gcgacggccg gcggcgtgat gtcccagccg acgacgtccg gcgtgatcgg
      961 atccgcatcg acatcgactg aatcctcggc gaccgtgacg gcggctttcg ggtacccttc
     1021 ggcgacggtc ggctcgagcg ggttgccatg ggc
//
LOCUS       DAOOLN010000993         1052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_193935_length_1052_cov_2.482447, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000993 DAOOLN010000000
VERSION     DAOOLN010000993.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193935_length_1052_cov_2.482447"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..653)
                     /locus_tag="PLW80_11450"
     CDS             complement(18..653)
                     /locus_tag="PLW80_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HPB67174.1"
                     /translation="MGSNTFVQAEVWTVAQTDQGNVVLIRPKGSDLVVPIFIGQLETQ
                     AILIGLGKVEMPRPLTHDLILSMLTMLDASLVRIEIRDLRDRTFFANVVLRGVEGEVS
                     IDARPSDAIALAVRTSAEVFVAEDIVEIAGVPVDMIRESPPDGTPGSEPGGSDTASEA
                     SGAPIIEDVSVLRQGSPDFERAVLSAELENAIEAEDYERAARIRDRLKELP"
     gene            731..>1052
                     /locus_tag="PLW80_11455"
     CDS             731..>1052
                     /locus_tag="PLW80_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPB67175.1"
                     /translation="MATGHYDKPDFWAEKARKEGYPARSVYKLDELDRKFGILRPGLR
                     VLDIGAAPGSWSLWALKRLNGSGFLSAVDLQPLGISPSYSNFYFIRGDLYDDAVKAAI
                     AERGP"
BASE COUNT          186 a          339 c          338 g          189 t
ORIGIN      
        1 gaataggtcg atcggggcta gggaagctcc ttgagccggt cccggatacg cgccgcgcgc
       61 tcgtaatcct ccgcctcgat ggcgttctcg agctcggcgc tcagcacggc ccgctcgaaa
      121 tctgggctgc cctggcgcag gaccgacacg tcttcaataa tcggcgcacc ggaggcctcg
      181 gaggccgtat cggatccgcc cggctccgac ccgggcgtcc catcgggagg ggactcgcgt
      241 atcatgtcga ccggtacgcc ggcgatctcg acgatatcct cggcgacgaa cacctcggcg
      301 ctcgtcctga cggccagggc tatcgcgtcg gagggccggg cgtctatcga cacctcgccc
      361 tcgacgcctc gcagcaccac attggcgaag aaggtgcggt cacgcaggtc gcgtatctct
      421 atcctgacca ggctcgcgtc gagcatggtc agcatggaca gaatcaggtc gtgggtcagc
      481 ggtcgcggca tctccacctt gccgaggccg atcaggatgg cctgcgtttc cagctggccg
      541 atgaagatcg gtacgacgag gtcggagccc ttgggccgga tgagtacgac gttgccttgg
      601 tcggtctggg ccacggtcca gacctccgct tggacgaagg tatttgatcc catacgcgct
      661 tccggatata gtatagaact tccgccggct gtatgtaagc cttagcggtc cactatcgct
      721 ggaggtctgc atggctacag ggcattacga taaaccggac ttctgggccg aaaaggccag
      781 gaaagaaggc tacccggccc gttcggtcta caagctcgac gagctggaca ggaaattcgg
      841 catcctgagg cccgggctgc gggtgttgga catcggcgcc gcgccgggca gctggagcct
      901 ctgggcgctc aagcggctga acggaagcgg cttcctgtcc gccgtcgacc tgcagcccct
      961 cggcatcagc ccgagctact cgaatttcta cttcatccgc ggcgacctct acgacgacgc
     1021 ggtcaaggcg gccatagccg aacgcggccc gt
//
LOCUS       DAOOLN010000994         1052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_193949_length_1052_cov_2.334002, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000994 DAOOLN010000000
VERSION     DAOOLN010000994.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193949_length_1052_cov_2.334002"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLW80_11460"
     CDS             complement(<1..283)
                     /locus_tag="PLW80_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67176.1"
                     /translation="MVTIEKVEAGAAAARAMPRLPRRIKDDALRLLALRLRERAPELF
                     EANEADVAAARASGASEAILTRLVYDGKKLEESIAMIDAVSRLPDPSGRT"
     gene            complement(347..>1052)
                     /locus_tag="PLW80_11465"
     CDS             complement(347..>1052)
                     /locus_tag="PLW80_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67177.1"
                     /translation="VIRAGVDAARFKAALASTRRRDSTAEGEAEAGSLAAAGCADGAP
                     EGGFGGWKAIRPGSSYPFCFVYATASSQANLKTSLGEAGYVAFRDRLRHIMQQGLAEA
                     DALLWMETDSSALYLVPPYAANASAAIEFCLRTLLGAPLIGYERLCLPFPLDFTFAIH
                     SGTTEFEPPGKTGNIVSEAVNFSYHLGVKRAEPGRVTISADAAGLAVPGRFSDLFVPA
                     GSFEGRSIFHSRRFWA"
BASE COUNT          184 a          343 c          356 g          169 t
ORIGIN      
        1 gcgttcgacc gctcggatcc ggtagccgcg agacggcgtc gatcatcgct atcgactcct
       61 cgagtttctt tccgtcgtag acgagcctcg taaggatggc ctccgaggcg cccgaggctc
      121 tcgccgccgc gacgtcggcc tcgttcgcct cgaacagttc gggagcccgt tcccgcagcc
      181 tgagcgccag gagccgcagc gcgtcgtcct tgatccggcg cggcaggcgc ggcatggccc
      241 tggccgcggc cgctccggct tcgaccttct ctatcgttac catgcgtaga aactatcaga
      301 aattcgacga ggagcgcaat cgggctatag gcggccgcgg cgaagatcag gcccagaagc
      361 gcctggaatg gaaaatggat cgcccctcga aggagccggc cggcacgaag agatctgaga
      421 atcgaccggg cacggccaag ccggcagcgt cggccgatat agtcactcga ccgggttcgg
      481 cccgtttgac gcctaggtgg tagctgaaat tgacggcctc cgagacgatg ttgccggtct
      541 tgccgggcgg ctcgaattcg gtagtccccg aatggatagc gaaggtaaag tcgagcggga
      601 acggcaggca tagtcgttcg tagccgatca gcggagctcc gagcagggtc cgcagacaga
      661 actcgatcgc cgccgaggcg ttcgccgcat atggcggcac gaggtagagc gcgctcgagt
      721 cggtttccat ccataggagc gcgtcggcct cggccaggcc ctgctgcatg atgtgccgga
      781 gcctgtctcg gaaagccacg taaccggctt ccccgaggct ggtcttcagg ttggcctgcg
      841 acgatgcggt cgcgtagacg aagcagaacg ggtacgacga gccaggccgt atggccttcc
      901 atccgccgaa gccgccctcc ggagcgccat cggcgcaacc ggccgcggcg agcgaaccgg
      961 cctcagcttc gccttcggcc gtggaatcgc ggcgccgggt cgaggccagc gccgccttga
     1021 acctggccgc gtcgacgccg gcccggatga cg
//
LOCUS       DAOOLN010000995         1050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_194560_length_1050_cov_2.585930, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000995 DAOOLN010000000
VERSION     DAOOLN010000995.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194560_length_1050_cov_2.585930"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PLW80_11470"
     CDS             complement(<1..805)
                     /locus_tag="PLW80_11470"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPB67178.1"
                     /translation="MLRLVFSRIKGFLLLLALLCAAGVVMFDALPVLMYPQTRRPMVT
                     VRFSHPGMSAIDFRDQYAADSIEPALTSMDNVDLVETTYSSDSSSITITFDWEIDGDA
                     ARDQVVAAMNGVRSYLPEDLRDAYSIRFREGENAGFIVMGVVSNATPPEELMPMLQAG
                     VESRLATIDGVDEYGIYGLEELSVEVTLDHEAMLAAGVSMLDVDAAFRAGLSPSPLGT
                     LREGDERYSLRLRRPTAGLESLGRLEIARDGLAVTRLEDIAEIDVRYDLP"
     gene            complement(806..>1050)
                     /locus_tag="PLW80_11475"
     CDS             complement(806..>1050)
                     /locus_tag="PLW80_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67179.1"
                     /translation="PVRGVFIDKALVVRRFSRSVVWVLRDDGTIGAVEVTLGRSYGDE
                     ALVLSGLEPGARYLAALTGAEREGMPALDLPSGRSR"
BASE COUNT          174 a          356 c          352 g          168 t
ORIGIN      
        1 acggtaggtc gtagcgcacg tcgatctcgg cgatatcctc gagccgcgtg accgccaggc
       61 cgtctcgggc tatctcgagg cggcctagcg actcgagccc ggccgtcggc ctccgtaagc
      121 gcaggctata gcgttcgtcg ccctcccgca gggtgccgag cggcgatggg gacaggccgg
      181 ccctgaaggc cgcgtccacg tcgagcatcg aaacgccggc ggccagcatc gcctcgtggt
      241 cgagggtcac ctcgacgctc agctcctcga ggccatagat gccgtactcg tcgacgccgt
      301 ctatcgtcgc caggcggctc tcgacgccgg cctggagcat tggcatcagc tcctcgggcg
      361 gcgtcgcgtt cgacacgacg cccatcacga tgaagccggc gttctcgccc tcgcggaagc
      421 gtatcgagta ggcgtcgcgt aggtcctcgg gcaggtagct cctgacgccg ttcatcgcgg
      481 cgacgacctg gtccctggcc gcgtcgccat cgatctccca atcgaaggtg atcgtgatcg
      541 agctcgagtc gctcgagtac gtcgtctcga cgaggtcgac attatccatg gacgtcagcg
      601 ccggctcgat cgagtcggct gcgtactggt cgcggaagtc tatcgccgac atcccgggat
      661 ggctgaagcg caccgtgacc atcggcctgc gggtctgggg gtacatcagt accggcaggg
      721 cgtcgaacat gacgacgccg gccgcgcata gcagcgcgag taacaacagg aagcccttga
      781 tacggctgaa gacgagcctg agcatctagc gggatctccc cgaaggtagg tcgagggccg
      841 gcatgccctc gcgctcggcg cccgtcaacg cggccaggta acgcgcgccg ggctcgagcc
      901 cggataggac gagcgcctcg tcgccgtagc tccggcccag ggtcacttcc acggcgccga
      961 tcgtgccgtc gtcgcgcagg acccagacga cgctcctcga gaagcgcctg acgacgaggg
     1021 ccttgtcgat gaagacgccg cgtacgggat
//
LOCUS       DAOOLN010000996         1049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_194850_length_1049_cov_2.738431, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000996 DAOOLN010000000
VERSION     DAOOLN010000996.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194850_length_1049_cov_2.738431"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PLW80_11480"
     CDS             <1..741
                     /locus_tag="PLW80_11480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB67180.1"
                     /translation="EMAEAGDRKRIWYERAKGRGLRVKLATDDAGRAVGMIQYAPIEA
                     SPALGEGLYYVYCVWVHGYTEGVGNFQGRGMGSRLLAAAEEDAKALGARGLVAWGLTL
                     PFFMRSKWFKRRGYVRADSDGALELVWKPFVDGAEPPRLPRRRKKPDDAPDEVVVTCI
                     ESGWCPAQNLACERARRAVSETGGDACLVELDGYDRATMLEWGAGDALFIDDAQIVTG
                     PPPSYESIRKKLERRIKKKKPKAARTGS"
     gene            complement(790..>1049)
                     /locus_tag="PLW80_11485"
     CDS             complement(790..>1049)
                     /locus_tag="PLW80_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67181.1"
                     /translation="YEYDDAGVRYLAATVTVENTGSSTISLSTFTVTAKTASRTYWKT
                     VTSDTRVLPGASIMVGAALAYADALETLVEGGLTVGDAFFE"
BASE COUNT          182 a          350 c          371 g          146 t
ORIGIN      
        1 gagatggccg aggccggcga ccggaagcgg atctggtacg agcgagccaa gggccgcggg
       61 ctccgcgtca agctggcgac tgacgacgcg gggcgcgccg tcggcatgat ccagtacgcg
      121 ccgatcgagg cgtcgcccgc cctgggcgaa gggctgtact acgtctactg cgtctgggtg
      181 cacggctaca cagaaggcgt cggcaatttc cagggacgcg gcatggggag ccggctgctc
      241 gcggccgccg aggaggacgc gaaggccctc ggcgcgcggg gcctcgtcgc ctggggcctg
      301 acgctgccgt tcttcatgcg gtccaagtgg ttcaagcggc gcggctacgt ccgcgccgac
      361 agcgacggcg cgctcgagct cgtctggaag cccttcgtcg acggcgccga gccgccccgg
      421 ctcccgaggc ggaggaagaa acccgacgac gcgccggacg aggtcgtcgt cacctgcatc
      481 gagagcggct ggtgcccggc ccagaacctc gcctgcgagc gcgccaggcg cgccgtctcg
      541 gaaaccggcg gcgacgcctg cctcgtcgag ctggacggct acgaccgcgc cacgatgctc
      601 gagtggggcg ccggcgacgc cctgttcata gacgacgccc agatcgtcac tgggccgcct
      661 ccgagctacg agtcgatcag gaagaagctc gagcggcgta tcaaaaagaa gaaaccgaag
      721 gctgcgcgga ccggctcctg acagcgtctc cgccctcggc tgaaaagaac ggctgaagag
      781 cggagtcgcc tactcgaaga acgcgtcgcc cacagtcagc ccgccctcga ccagggtctc
      841 caaggcatcg gcgtaggcca gcgcggcgcc caccatgatc gaggcgccgg gtagcacgcg
      901 cgtatcgctc gtcaccgtct tccagtaggt ccgcgaggcg gtcttagccg ttacggtaaa
      961 ggttgatagt gaaatagtcg agctgcccgt gttctcgacg gtcacggtcg cggcgaggta
     1021 ccgaactccg gcatcgtcgt actcgtagg
//
LOCUS       DAOOLN010000997         1048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_195077_length_1048_cov_3.686808, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000997 DAOOLN010000000
VERSION     DAOOLN010000997.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195077_length_1048_cov_3.686808"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1048
                     /locus_tag="PLW80_11490"
     CDS             <1..>1048
                     /locus_tag="PLW80_11490"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPB67182.1"
                     /translation="LEGGYIEDAPLRLDGEGVSIARATGAGTTFTVVAEAPSGLYDGF
                     GRSARNVALAGLLAAAGLASVLSLRASASVTEPVLEMARSMRLVEDGDLSIRIKPSGD
                     DELGDLARSFNAMTAEVDALLRREVERQELLREAELRALAAQMNPHFLHNTLASIKSL
                     AKLGRSGEIAEVVSRLGKLLRSGSGRRDGLSTVSESLSSVRDYLSIEKLRFGERFSFE
                     LDVDPALESVTLPPLTLEPLAENALTHGLERKRGPGSLRIEGRLDGGDAVISFADDGP
                     GMDAEAIARLSEALEEAKAPAGEHGMGLLGTNRRLALEYGAGYGIRIRSGGPSGAGFV
                     ATVRIPSGLATPKGE"
BASE COUNT          165 a          355 c          371 g          157 t
ORIGIN      
        1 gctcgagggc gggtacatcg aagacgctcc gctcaggctc gacggcgagg gcgtttccat
       61 agcccgcgct acgggagccg gaacgacatt cacggtggtc gccgaggcgc cgagcggcct
      121 ctacgacggc ttcggccgct cggcgcgcaa cgtcgcgctg gccgggctgc tcgccgcggc
      181 gggcctggcg tcggttctgt ccctgcgcgc ctcggcgtca gttaccgagc cggtgctgga
      241 gatggcccgc tcgatgcgcc tcgtcgagga cggcgacctc tcgatccgta tcaagccttc
      301 cggggacgac gaactaggcg acctggcccg ctcgttcaac gcgatgaccg ccgaggtgga
      361 cgcgctcctc cggcgcgaag tggagcgtca ggagctgctc agggaggcgg agctacgcgc
      421 cttggcggcc cagatgaacc cgcacttcct ccacaacacg ctcgcctcga tcaagtcgct
      481 ggccaagcta ggccgttccg gcgagatcgc ggaggtagtc tccaggctcg gcaagctcct
      541 gcgatccggt tccgggcgac gcgacggcct gtccaccgtg agcgagagcc tgtcgtcggt
      601 gcgcgattac ctctccatcg agaagcttcg cttcggagag cgcttcagct tcgagctgga
      661 cgtcgacccg gccctggagt ccgtgacgct accaccgctg acgctcgagc cactcgcgga
      721 gaacgccctg acgcacgggc tggagcgtaa gcgcgggccc ggatccctga ggatcgaggg
      781 ccggttggat ggcggcgacg ccgtgatctc cttcgccgac gacggtccag gtatggatgc
      841 cgaggcgata gcccggctat cggaggccct cgaagaagcc aaagctccgg cgggcgaaca
      901 cggcatggga ctcctgggca cgaaccgacg cctggccctc gagtacggcg caggctacgg
      961 catcaggata cggagcggcg gaccgtccgg cgcgggcttc gtcgcgacgg tcaggatccc
     1021 gtcgggactc gcgactccca agggggag
//
LOCUS       DAOOLN010000998         1048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_195127_length_1048_cov_2.871098, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000998 DAOOLN010000000
VERSION     DAOOLN010000998.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195127_length_1048_cov_2.871098"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PLW80_11495"
     CDS             <1..330
                     /locus_tag="PLW80_11495"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPB67183.1"
                     /translation="TVQRVLLETWLDAYGSFIEKGAVVVGVSPDESGSHARFKAKYGL
                     PFVLLADPEHRALEAYGVWAEKSMYGRKYMGVVRSTYVIGEDGVILKAFPKVSPEGHA
                     VEILAAL"
     gene            528..1013
                     /locus_tag="PLW80_11500"
     CDS             528..1013
                     /locus_tag="PLW80_11500"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HPB67184.1"
                     /translation="MKLYFAAPLFCEAERRYNADLAEKVEALGFEVFLPQRDGVVGAS
                     ETGDGGDAGRDDASGDGAVMRRARAIFELDLERIKATDIFFYLLDGRVPDEGAAVALG
                     IAFADKAARRPDRLLLGLHTDGRAAFPDAALNPMLAASLDRVFADERSLLAYLDGLPR
                     R"
BASE COUNT          165 a          366 c          337 g          180 t
ORIGIN      
        1 accgtccaac gcgtgctcct ggaaacttgg ctcgacgcct acggctcgtt catcgagaag
       61 ggcgccgtgg tcgtcggcgt cagcccggac gagagcggct cccacgcccg cttcaaggcc
      121 aagtacggcc tgcccttcgt cctgctggcc gaccctgagc accgcgcgct cgaggcctac
      181 ggggtctggg ccgagaagtc gatgtacggc cgcaagtata tgggcgtcgt ccgctcgacc
      241 tacgtcatag gcgaggacgg cgtcatactc aaggccttcc ccaaggtatc tcccgagggc
      301 cacgccgtag agatcctggc cgcgctgtga cctgacctgc gggcctcggc ttgcgcctcg
      361 accgcttcag gcgtatcata ccgactagaa acacggaagg aggcgcctat gcgtagcaac
      421 gccagcgctc cgatccattg ctcgcggtcg gtaagctgat ctcatccggc tgctaacgat
      481 acgccggggc cttcctgggg ccccggcctt tttattaagg acgtactatg aagctctatt
      541 tcgccgcgcc cctgttctgc gaggccgagc ggcgctataa cgccgacctc gccgagaagg
      601 tggaagccct cggcttcgag gtattcctgc cgcagcgcga cggcgtggtc ggcgcttccg
      661 agacgggcga cggcggcgac gcgggccgcg atgacgcaag cggcgatggc gccgtcatgc
      721 ggcgggcgag ggccatcttc gagcttgacc tcgaacgcat caaggctacc gacatattct
      781 tctacctgct cgacggccgg gtgcccgacg agggggccgc ggtcgctctc ggcatagcct
      841 tcgccgacaa ggcggcgcga cggccggatc ggctgctcct cgggctccat accgacggcc
      901 gcgccgcgtt cccggacgcc gccctgaatc cgatgctcgc cgcgtctctc gaccgcgtct
      961 tcgcagacga acgctcgctc ctggcctacc tcgacgggct accgcgccga tgacgctgat
     1021 acgcggaggc ctggtcgtcg acggtacc
//
LOCUS       DAOOLN010000999         1046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_195654_length_1046_cov_3.259334, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010000999 DAOOLN010000000
VERSION     DAOOLN010000999.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195654_length_1046_cov_3.259334"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..476)
                     /locus_tag="PLW80_11505"
     CDS             complement(168..476)
                     /locus_tag="PLW80_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HPB67185.1"
                     /translation="MSDDIGASAPRDDDPLHGVTLRMMVERLSERIGWAGLAEKVPVR
                     CFTNDPSVDSSLKFLRRTPWAREKVELLYLYAFHPKRARELAVKRRGGERPGRLDPRR
                     "
     gene            610..>1046
                     /locus_tag="PLW80_11510"
     CDS             610..>1046
                     /locus_tag="PLW80_11510"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HPB67186.1"
                     /translation="MQNEIVSPMDLLDDSGRITREGWARYPYWRYDRAKIRSNALRIK
                     EWDYYSVLSQDGRFGIALTMSDLGYAGLFAICFLDFERRTSHQVDTLSVLPLGRTGFP
                     PVSDEGRISFGDKKLSMTFVYAGGVRTLSFSAPGLKNAEGALIA"
BASE COUNT          180 a          371 c          332 g          163 t
ORIGIN      
        1 gctcatggcg ccagctcccg cggcgaagcg gcccgggccg gcccggccga ccggtgcccc
       61 gccgaccagt gcccgactgc cgtcctccgt cgaacggcta tcgcccctgc gcaaggccgt
      121 cgtcatggcg gagctgctcg gcccgcccaa ggcgctcagg taggtcgtca gcgccgagga
      181 tcgagacgcc ccggacgctc cccgcctcgc cgcttgaccg ccagctcgcg ggcccgtttc
      241 ggatggaagg cgtacaggta gaggagctcg accttctcgc gggcccaggg cgttcgccgc
      301 aggaacttga ggctcgagtc gacgctcgga tcgttcgtaa aacaacggac cggcaccttc
      361 tccgcgaggc cggcccagcc gatacgctcg gacaggcgct cgaccatcat gcgcagggta
      421 acgccgtgga gagggtcgtc gtcccgcggc gcggacgcgc caatatcgtc tgacacggaa
      481 tgctccttac ggcgctcgcg ggcgccagag cacactatac ctcgctacgg ccggccgacg
      541 ccaggcgttt tacgctggca aagccggcga acgcgtcgta tagtagtcgc atcgacggag
      601 gccggcgaca tgcagaacga aatcgtttca ccgatggacc tactcgacga ctcgggcaga
      661 atcacccgag agggctgggc ccgctacccc tactggcgct acgaccgcgc gaagatacgc
      721 tcgaacgcgc tgcgcatcaa ggaatgggac tactactcgg tcctgtcgca ggacggccgc
      781 ttcggtatag ccctgaccat gagcgacctg ggctacgccg gcctgttcgc gatctgcttc
      841 ctcgacttcg agcggcgcac gtcccatcag gtcgacacgc tcagcgtgct accgctcggc
      901 cgcaccggct ttccgccagt atccgacgaa ggcaggattt cattcggcga caagaagctg
      961 tcgatgacct tcgtgtacgc cggcggcgtg cgcaccctat cgttcagcgc tcccggcctc
     1021 aagaacgccg aaggcgcgct gattgc
//
LOCUS       DAOOLN010001000         1046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_195692_length_1046_cov_2.735621, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001000 DAOOLN010000000
VERSION     DAOOLN010001000.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195692_length_1046_cov_2.735621"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..>1046
                     /locus_tag="PLW80_11515"
     CDS             79..>1046
                     /locus_tag="PLW80_11515"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB67187.1"
                     /translation="MTRIEDRERVLFLYLPTGGGHLSNCKAISRRVAERYGAETLAYD
                     PVSARSPLGKLFLEGGYRFMSLRLPWMWNLLFELNDNPFIMRLSQGSARRFSRYGLRY
                     IIDEWKPTRIVCLHHLLSPAVAAALEGREPIPVTVVATDPFVPPKLWAHGHEYPIVCM
                     SEEARDSFLAMGVDESRLRTYSIIIGEKFERRMDEEARAAFKRSVGLDPERPLVLIAG
                     GGDGMRGADRILDELCSSSLDYQVALVCGRDERTRKRAEAKALEYRIRGRRVVVFGFT
                     DRMYELMESADAIVSKAGPAVLGEVLAAGKPNVIAFYIPGQEKPNVR"
BASE COUNT          161 a          333 c          376 g          176 t
ORIGIN      
        1 tgcggccgcc gcgttggacg ctcgggtgtc gatagcggta tcgtatcgct aggcgcggga
       61 gccgcgccaa ggaagaccat gaccaggata gaagataggg agcgcgtgct gttcctgtac
      121 ctgcccaccg gcggcgggca cttgtccaac tgcaaggcga taagccgccg cgtggccgag
      181 cgctacgggg ccgagacgct ggcctacgat ccggtctcgg cgcgttcgcc gctcggcaag
      241 ctgttcctgg agggcgggta ccgttttatg tccctgcgcc tgccctggat gtggaacctc
      301 ttgttcgagc tgaacgataa tcccttcatc atgcggctgt cgcagggcag cgccaggcgc
      361 ttctcgcgct acggtctgcg ctacatcata gacgagtgga agccgacgcg catcgtctgc
      421 ctgcaccacc tcctgtcccc ggcggtggcc gccgcgctcg aggggcggga gccgataccg
      481 gtgacggtgg tggccaccga ccccttcgtg ccgcccaagc tctgggccca cggccacgag
      541 taccccatcg tctgcatgtc cgaagaggct cgcgatagct tcctggcgat gggcgtggac
      601 gagtcgcgtc tgcggaccta ttcgatcatc atcggggaga agttcgagcg gcggatggac
      661 gaggaggcgc gcgccgcctt caagcgctcg gtagggttgg acccggagcg gccgctggtg
      721 ctgatagccg gcggcgggga cggcatgcgc ggcgccgacc gcatcctgga cgagctgtgc
      781 tcctccagcc tggactacca ggtggcgctg gtctgcggcc gcgacgagcg tacgcgcaag
      841 cgggcggagg ccaaggcgct cgagtaccgg atccggggcc gccgggtcgt ggtgttcggc
      901 tttaccgacc gcatgtacga gctcatggag tcggccgacg cgatcgtgtc gaaggccggc
      961 cccgcggtgc tcggcgaggt gctggctgcg ggcaagccga acgtcatagc cttctatata
     1021 cccggccagg agaagccgaa cgtgcg
//
LOCUS       DAOOLN010001001         1045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_195946_length_1045_cov_3.314141, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001001 DAOOLN010000000
VERSION     DAOOLN010001001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195946_length_1045_cov_3.314141"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PLW80_11520"
     CDS             complement(<1..597)
                     /locus_tag="PLW80_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB67188.1"
                     /translation="MKTGSNRYDVCVIGAGVCGANIARRLSAYELDVALVDKEADVSF
                     GTSKANSGIVHGGFHHNKKYLKARLELQGMMMFDRLQQELDFPFKRCGIVVAALHEDE
                     MRAIEQLYMQGVDNGVIGIEMCSRERILELEPKLNPDTLGGLWVPGGGIVEPYRFVFA
                     LVESARKNGVEIMTGFKVEHAARAGDEWTVRAADGRVVK"
     gene            709..>1045
                     /locus_tag="PLW80_11525"
     CDS             709..>1045
                     /locus_tag="PLW80_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67189.1"
                     /translation="MGFIQRSCVRSIHGLLASHRLVLVEGPPRSGRSTIAAQVAAGAP
                     GGGILVDARRPEGRDVLADPASASAARLIVVDGAGRREAERVAASLSAFGEAERTPRF
                     ALIGGPFGHA"
BASE COUNT          164 a          347 c          339 g          195 t
ORIGIN      
        1 cttgacgacg cggccgtcgg ccgcgcggac cgtccactcg tcgccggcgc gcgccgcgtg
       61 ctcgaccttg aagccggtca tgatctcgac gccgttcttg cgcgcgctct cgacgagggc
      121 gaacacgaag cggtacggct cgacgatgcc gccgccgggg acccagaggc cgcccagggt
      181 gtcggggttg agcttgggct ccagttcgag gatgcgttct cgggagcaca tctcgatgcc
      241 gatgaccccg ttgtcgacgc cctgcatgta cagctgctcg atggcgcgca tctcgtcctc
      301 gtgcagggcc gcgacgacga tgccgcaacg cttgaacggg aagtcgagct cctgctgcaa
      361 cctgtcgaac atcatcatgc cctgcaactc gagccgtgcc ttgaggtact tcttgttgtg
      421 atggaagccg ccgtgcacga tgcccgagtt ggccttggac gtgccgaagg atacgtcggc
      481 ttccttgtcg acgagcgcca cgtcaagctc gtaggccgac aggcgcctcg ctatattggc
      541 gccgcagacg ccggcgccga tcacgcagac gtcgtagcga ttcgatccag tcttcacccc
      601 tgaccccctt gctacctatc agtataggac gaaaatggac cctagcaaga tgaacgctcg
      661 atgctagcca cttttattac cgcattttaa acgctatgct ttcatacaat ggggtttatt
      721 cagcgttcct gtgtccgaag catccatggg ctgctcgcca gccatcggct cgtgctcgtc
      781 gaggggccgc cgcgctccgg caggtcgacg atagcggcgc aggtggcggc cggcgctccg
      841 ggcggcggca tactcgtcga cgcgcgcagg ccggaggggc gcgacgtcct cgccgatccg
      901 gcgtccgcct cggccgctcg actgatcgtg gtcgacggcg ccgggcggcg cgaggccgag
      961 cgcgtagccg ccagcttaag cgccttcggc gaggctgaac gtacgccgcg cttcgctctt
     1021 ataggcgggc cgttcggcca tgcgg
//
LOCUS       DAOOLN010001002         1045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_195989_length_1045_cov_2.693939, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001002 DAOOLN010000000
VERSION     DAOOLN010001002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195989_length_1045_cov_2.693939"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PLW80_11530"
     CDS             <1..268
                     /locus_tag="PLW80_11530"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPB67190.1"
                     /translation="ALGLFAFSCGTASGVLFGKLMCAWTKGKVNPLIGSAGVSAVPMA
                     ARVSQVVGQRENPDNYLLMHAMGPNVAGVIGCAVAEGVMLSFLG"
     gene            complement(521..>1045)
                     /gene="hflC"
                     /locus_tag="PLW80_11535"
     CDS             complement(521..>1045)
                     /gene="hflC"
                     /locus_tag="PLW80_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HPB67191.1"
                     /translation="TGDAEGSDSLRQLTQTDASYDTIDKGRRALSGDMLAMAQQLTPE
                     YGIEIIDVIPRQIRYSDELTESVYNRMIKERNQIAQAFRSFGEGKKSEWMGKLENEQR
                     SILSKAYEEAEVIKGTADARATKIYADAYGRDRGFFDFWRAIESYRTTMPKFTKTLST
                     DMSYFDYLYGPKGR"
BASE COUNT          160 a          318 c          335 g          232 t
ORIGIN      
        1 cgccctcggc ctgttcgcgt tttcctgcgg caccgccagc ggcgtcctgt tcggtaagct
       61 catgtgcgcc tggacaaagg gcaaagtcaa cccgctgatc ggttcggccg gtgtctcggc
      121 cgtgccgatg gccgcccgcg tctcccaggt tgtggggcag cgcgagaacc ccgacaacta
      181 cctcctcatg cacgccatgg gcccgaacgt ggctggcgtc atcggatgcg cggtagcgga
      241 aggcgtcatg ctgtcgttcc tgggctagag cgtctctcgt ctccgttcag ccggccgtcg
      301 ggcgctacgc tcggcggccg gttttttatt ctaccgggac cagaggttga tcccgttcac
      361 ccggcaagac gatgaccttg ctcatggcgc agatgccgcc gatcgagcta ctataccggg
      421 atgaatgccg agcccccgag ctgcgaccgc gtagatgatg gggccttagc ataaaaaagc
      481 ccgcccctga cggggcgggc ttctttttat cgaactacga ctaacgtccc ttgggtccgt
      541 agaggtagtc gaagtagctc atgtcggtcg acagggtttt cgtgaacttg ggcatcgtgg
      601 tcctgtagga ctcgatcgct cgccagaagt cgaagaagcc gcggtcgcgg ccgtaggcgt
      661 cggcgtagat cttggtagcc cgagcgtcgg cggttccctt gatcacctcc gcttcctcgt
      721 aggccttgga aagtattgaa cgctgttcgt tctcgagctt gcccatccac tcggacttct
      781 tgccctcgcc gaaggagcgg aaggcctggg cgatctggtt gcgttccttg atcattctat
      841 tgtagacgct ctcggttagc tcgtcggagt agcggatctg gcgcggtatc acgtcgatga
      901 tctcgatgcc gtactcgggc gtcagctgct gggccatcgc gagcatgtcc ccggatagcg
      961 cgcggcgtcc cttgtcgatg gtgtcatagg acgcgtcggt ctgggtcagc tgcctgagcg
     1021 agtcggagcc ctcggcgtcg ccggt
//
LOCUS       DAOOLN010001003         1044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_196242_length_1044_cov_3.680485, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001003 DAOOLN010000000
VERSION     DAOOLN010001003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196242_length_1044_cov_3.680485"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PLW80_11540"
     CDS             <1..840
                     /locus_tag="PLW80_11540"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB67192.1"
                     /translation="DRSVLNDSMRIIALVDDLCPKGGFRGEHGLSYYIETGSAKLLFD
                     AGQTDALVANAETLDVDLAGVDAVALSHGHYDHGGGLGPLFASIAPTRPVVYAGRGYS
                     VRKWARNDDGLRDIGLPESSYPPLGSAAVEVDGLSNPFPGVFLQAGADRPDSEAANPR
                     FRLIDGGIERVDPFDDELSLILEAADGIVVVTGCAHRGIVNIVKAAMGAFPGRSLSAI
                     VGGFHLGGEPDETLSRVAGELAALAPKRILCGHCTGVRGYAAIREAFSGVEWLSCGMQ
                     VEL"
BASE COUNT          165 a          335 c          356 g          188 t
ORIGIN      
        1 gaccggagcg tcctcaatga ctccatgaga atcatcgcgt tggtagacga cctgtgcccg
       61 aagggagggt tccgcggcga gcacggcctc tcgtactata tcgagaccgg ctcggccaag
      121 ctcctattcg acgcgggaca gaccgatgcg ctggtagcga acgcggagac gctcgacgtc
      181 gacctggccg gcgtcgacgc cgtcgcgctc agccacgggc actacgacca cggaggcggg
      241 ctcgggccgc tcttcgcttc gatcgcgccg acgcgcccgg tcgtgtacgc cggccggggc
      301 tactccgtcc gcaagtgggc caggaacgac gacggcctgc gcgatatagg cctgcccgag
      361 tcgtcgtatc ctccgctggg ctccgcggcc gtcgaggtcg acgggctgtc gaacccgttc
      421 ccgggcgtct tcctgcaggc cggggcggat cgccccgata gcgaggccgc gaatccgagg
      481 ttccgcctga tcgacggcgg gatcgagcgc gtcgacccgt tcgacgacga gctgtcgctg
      541 atcctcgagg cggccgacgg tatcgtcgtc gtcaccggct gcgcccatcg cggcatcgtc
      601 aacatcgtca aggcggctat gggcgcattc ccgggacggt cgctgtcggc tatagtcggc
      661 ggcttccacc tgggcggaga gccggacgag acgctgtcgc gcgtggccgg cgagttggcg
      721 gcgctggctc ctaagcgtat cctgtgcggg cactgcaccg gcgttcgcgg ctacgcggct
      781 atcagagagg cgttcagcgg cgtcgagtgg ctttcatgcg gtatgcaagt cgagctgtga
      841 gccgcgcgac cagtatatcg tcgccttgac cgcggaatga tctatagata cattgtccta
      901 acattcgggc cataggccga atcacgtcac tcgtcatgtc ccgttcggcg gaagcgctcg
      961 ggagagagga tttatcatgt cccagaaggt tcaggcttcg ttcttcgacg cgaagggcgt
     1021 cgcgttcgtc ggctactcca agcg
//
LOCUS       DAOOLN010001004         1044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_196277_length_1044_cov_3.035389, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001004 DAOOLN010000000
VERSION     DAOOLN010001004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196277_length_1044_cov_3.035389"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PLW80_11545"
     CDS             <1..300
                     /locus_tag="PLW80_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67193.1"
                     /translation="LRKGVVPKEYSRFGSSVEELFADLRLLKSSYGDDAAGIPTGAIG
                     VFSYLHRVADGLRMLMALNRKFALRYIARDDIVPLTDEAARATGLATYAELLDRA"
     gene            517..>1044
                     /locus_tag="PLW80_11550"
     CDS             517..>1044
                     /locus_tag="PLW80_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67194.1"
                     /translation="MQRKGKGAASVLIVLVVAAATSCGLLDALLGTDLEGDGSPTEVT
                     GLSVAGTVSYDTAARDVVGDGSYAYVATYTYSGDYGGVSVVDVSTPASPTKRGAYYPT
                     SSSTSLYASYIAKSGDSVYIDGGSVRYFAKVSVSTPSSPYRVFQYDGSNSLCSGLIAD
                     GTTLYYNSYHGGCIDI"
BASE COUNT          188 a          338 c          317 g          201 t
ORIGIN      
        1 ctcaggaagg gcgtcgtgcc caaggaatac tcgcgcttcg gttcgagcgt cgaggaactg
       61 ttcgccgacc tgcgcctcct caagtcgagc tacggcgacg atgcggcagg catacccacc
      121 ggagccatcg gcgtattctc gtacctgcac cgcgtcgccg acggcctcag gatgctgatg
      181 gccctcaaca ggaaattcgc gctgcgctac atagcccgcg acgacatcgt gccgctgact
      241 gacgaggccg cccgcgccac cggactcgcc acctacgcgg aactgctcga ccgggcctga
      301 tagcctacgg ccacgcgtcg tcggctctgc gcgggtcgac gatgaggccg gcggcgatta
      361 gctcctcgtt atgtttgaat agctcaatgc tccgcgctat gctttcctgg tgacagggct
      421 tgagagcccg agatccggct gggattcttg ttgcacctat cagccacgct tgcttccccg
      481 ctagtcggct ccgttcgccc tggaaagagg ggaagcatgc agcgtaaggg taagggcgcg
      541 gctagcgttc taatcgttct cgtcgtcgcc gcggctacat catgcgggtt gctagacgcg
      601 ctcctgggca ccgatctcga gggagacggc tcgccgacag aggtcaccgg attatcggtc
      661 gcgggcaccg tatcatacga cacggccgcg agggacgtcg tcggtgacgg ctcgtacgcg
      721 tacgtagcca cgtataccta ttcgggcgac tatggcggcg tctcggtcgt cgacgtcagt
      781 actcccgcat caccgacgaa gcggggggcg tactatccga ccagctcgag tacctcgctc
      841 tacgcctcgt acatcgccaa gagcggggac agcgtctata tagacggcgg tagcgtgcgt
      901 tacttcgcta aggtcagcgt ctcgacgccg agctctccct acagggtctt ccagtacgac
      961 ggatcgaaca gcctctgttc cggccttatt gccgacggca cgaccctgta ctacaacagc
     1021 taccacggag gctgcatcga tatc
//
LOCUS       DAOOLN010001005         1043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_196540_length_1043_cov_3.458502, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001005 DAOOLN010000000
VERSION     DAOOLN010001005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196540_length_1043_cov_3.458502"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /gene="amrB"
                     /locus_tag="PLW80_11555"
     CDS             <1..417
                     /gene="amrB"
                     /locus_tag="PLW80_11555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPB67195.1"
                     /translation="LLFPGARLIPILACGDASVAAGLARSIDAVLGADLGRTLIVASS
                     NMAASIVAADSSRRTDELLQSIARGAWRDVASRDDIGGSVAIAAAMAVAPMQDRRFAL
                     FGRGDSLGHEKSAAERVVQYASAAWYRLAASPAEAP"
     gene            414..980
                     /gene="amrA"
                     /locus_tag="PLW80_11560"
     CDS             414..980
                     /gene="amrA"
                     /locus_tag="PLW80_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HPB67196.1"
                     /translation="MSFELSSEDKGALLATAREAIESRLLGRQPRWPSGGPALQVRLG
                     AFVTLHKRSSYGRRLLRGCIGRMSADKALVLTVRDMALAAAFEDPRFPPLIDRELEAI
                     DVEISVLSPFEPCAPDDVVPGVHGVLLRRGYSSGVFLPQVATEQGWDREALLEELCVK
                     AGVESGSYRAPDASLYRFTAMVFGELEP"
BASE COUNT          143 a          364 c          356 g          180 t
ORIGIN      
        1 ctccttttcc cgggagccag gctcattccg atactggcct gcggcgacgc ctcagtcgcc
       61 gcgggtctgg caaggtccat cgacgccgtg ctcggagccg accttgggcg gaccctcatc
      121 gtcgcctcga gcaacatggc ggcgtccatc gtcgccgccg actcgtccag gcgtaccgac
      181 gagctcttac agagcatcgc tcgaggtgcc tggcgggacg tggcgtcacg cgacgatata
      241 ggcggctcgg tggccatagc cgcggccatg gccgtcgcgc cgatgcagga tcggcgcttc
      301 gcgctgttcg gccggggcga ttcgcttggc cacgagaagt cggccgccga gcgcgtggtg
      361 cagtacgcct cggcggcctg gtaccggctc gccgcctcgc ccgcggaggc gccatgagct
      421 tcgagctatc gagtgaagac aagggcgcgc tactcgcgac ggcgcgggaa gccatcgagt
      481 cccgactgct tggccgccag ccccgctggc cgagcggcgg gcccgccctc caggttcggc
      541 tcggcgcctt cgtcaccctg cacaagcggt cgtcgtacgg ccggcggctg ctacgcggct
      601 gcataggcag gatgagcgcc gacaaggccc tcgtccttac cgtgcgcgac atggcgttgg
      661 ccgccgcctt cgaggatccg cgctttccgc ccctgatcga ccgcgagctg gaggctatag
      721 acgtcgagat ttcggtgctg tcgcccttcg agccatgcgc tccggatgac gtcgtcccag
      781 gcgtgcatgg ggtgctcctg aggcggggtt attcctccgg cgtcttcctg ccccaggtcg
      841 ccacagagca gggctgggat cgcgaggctc tgctcgagga gctatgcgtc aaggccggcg
      901 tcgaaagcgg ctcctacagg gcgcctgacg cgagtctcta tcgatttacc gccatggtct
      961 tcggggaact cgagccctga cgggtctcga gcacggtaac gcgccgcgcc tcctcgtcga
     1021 tccgtagccg cgcgaagccc gct
//
LOCUS       DAOOLN010001006         1041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_197318_length_1041_cov_2.200811, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001006 DAOOLN010000000
VERSION     DAOOLN010001006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197318_length_1041_cov_2.200811"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..686
                     /locus_tag="PLW80_11565"
     CDS             234..686
                     /locus_tag="PLW80_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67197.1"
                     /translation="MKRVCASLILASLCVVAAIGQSNDFVDGLIASDSVSVGQAAYLV
                     LVASDNLGEDADAARAFELLGTFGWAPTGASADVPILLKDYAYLLMSAFGLRGGLMYA
                     LFPGPRYAYRQLVSSFVIQGRSDPDMAVSGPVAVRMLGRVFDVKGIAE"
     gene            683..>1041
                     /locus_tag="PLW80_11570"
     CDS             683..>1041
                     /locus_tag="PLW80_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67198.1"
                     /translation="MNRLRSSRTAPRALACLALVAVLCAGNAFALDFGLTLSNDSELS
                     GGGGLSLSQSDSAVLWLSLPIGASSSIYASAVYEFSGDFTYLPSYSDRVRWYSITAGR
                     VEWEGFVGLSESMGLGWS"
BASE COUNT          159 a          350 c          306 g          226 t
ORIGIN      
        1 acgatggccc cgtcttcggc tacggccggc ttaagccgca ggaagaggtc ataggctcca
       61 tgctcgaggt ctcgccacgc cggcaccagg tagagcaggg agaacgcgat gcctatggcg
      121 atcggtacga ctataagcct gcgcgatact tttcgttttg gcatcggagc ctccatgctg
      181 ataaactttt gttaccgtag tatactacgc atattcccat ttaaaggtgg catatgaaaa
      241 gagtatgtgc atcccttatc ctggcgtcgc tatgcgtcgt cgccgccatc gggcagtcga
      301 acgacttcgt cgacggcctg atagcgtcgg actcggtcag cgtcggccag gcggcctacc
      361 tcgtgctggt cgcctcggac aacctcggcg aggacgccga tgccgcccgc gccttcgagc
      421 tcctcggcac cttcggctgg gctccgacgg gcgcttccgc cgacgtgccc attctactca
      481 aggactatgc gtatctcttg atgagcgctt tcggccttcg gggcggcctc atgtacgcgt
      541 tgttccccgg gcctcgctat gcctataggc agctcgtctc gagcttcgtc atccagggcc
      601 gttccgaccc tgatatggcc gtctccggcc cggtcgccgt caggatgctc ggccgcgtgt
      661 tcgacgtgaa gggaatagcc gaatgaaccg cctgagatca agccgcacgg cccctagggc
      721 cctcgcctgt ctcgccctcg tcgcggtctt atgcgccggc aacgccttcg ccctcgattt
      781 cggccttacc ctatccaacg actccgagct gtccggcggc ggcggcctat cgctgtcgca
      841 gagcgactcc gccgtgttat ggctgtcgct accgataggc gcctcctcgt ccatctacgc
      901 gtccgccgtc tacgagttct cgggcgactt cacctacctg ccctcgtact cggaccgcgt
      961 ccgctggtat tcgatcaccg ccggacgcgt cgagtgggaa ggcttcgtcg gcctgagcga
     1021 gtcgatgggc ctcggctggt c
//
LOCUS       DAOOLN010001007         1039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_197825_length_1039_cov_4.284553, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001007 DAOOLN010000000
VERSION     DAOOLN010001007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197825_length_1039_cov_4.284553"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLW80_11575"
     CDS             complement(<1..233)
                     /locus_tag="PLW80_11575"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPB67199.1"
                     /translation="MTRGRRARGLASLACLAALAAGAVGPAAAADVVRFVNLGLGDGL
                     ANASVSSMVQDAAGFIWIGTQGGLHRWDGLSFTL"
     gene            complement(230..>1039)
                     /locus_tag="PLW80_11580"
     CDS             complement(230..>1039)
                     /locus_tag="PLW80_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HPB67200.1"
                     /translation="PRNAETAIAVEAEVRAAGAVPATIAIIGGRLKAGLSESEIGLLG
                     ERGHAVAKVSRRDIGRIVAAKADGATTVAGTMIVAAMAGIKVFATGGIGGVHRGAELT
                     MDVSADLDELGRTDVAVVCAGAKSILDIPMTLEYLETKGVPVVVYRGDEFPAFYTSRS
                     GLRAELRMDSAAEIAAMLKAKWSLGLSGGAVIANPIPTEYEMRPEVINAAIERALAEA
                     KAAGVRGKDVTPYMLAKVKDLTGGDSLESNIALVMNNARLAAALAMEYARL"
BASE COUNT          146 a          365 c          365 g          163 t
ORIGIN      
        1 agggtgaacg atagcccgtc ccagcgatgg agcccgccct gggtgccgat ccagatgaag
       61 ccggcggcgt cctggaccat ggacgagacg ctcgcgttgg ccaggccgtc gcccaggccg
      121 aggttgacga agcgcacgac gtccgcggcc gccgcggggc ctaccgctcc cgcggccagg
      181 gcggcgaggc aggccagcga ggcgaggccc cgggcgcggc gccccctcgt catagtcgag
      241 cgtactccat cgccagcgcg gcggcgagcc tggcattgtt catgacgagc gcgatgttcg
      301 attcgaggct gtcgcctccg gtcaaatcct taaccttggc caacatgtag ggcgtgacgt
      361 ccttgccgcg gacgccggcg gccttggcct cggcgagggc gcgctcgatg gcggcgttta
      421 tgacctccgg ccgcatctcg tactcggtcg gaatcgggtt ggctatcacg gcgccgcccg
      481 acaggccgag cgaccacttg gccttgagca tcgcggctat ctcggccgcg gagtccatgc
      541 gtagctcggc gcgcaggcca gagcggctcg tgtagaaggc cgggaattcg tcgcccctat
      601 agacgacgac gggcacgccc ttggtctcca ggtactcgag cgtcatcggg atatccagga
      661 tgctcttggc gccggcgcag acgacggcga catcggtgcg gccgagctcg tcgaggtcgg
      721 cggacacgtc catcgtcagc tcggcgccgc ggtgtacgcc gcctatgccg ccggtcgcga
      781 acaccttgat gccggccatg gcggccacga tcatcgtgcc ggccaccgtg gtcgccccgt
      841 ccgccttggc cgcgacgata cgacctatgt ctcggcggct gaccttggct acggcgtggc
      901 cccgttcgcc gaggaggcct atctccgact cggacaggcc ggccttcagg cggccgccga
      961 tgatcgcgat ggtcgccggc acggcgccgg cggctcgcac ctcagcctcg acggcgatgg
     1021 ccgtctcggc attccgcgg
//
LOCUS       DAOOLN010001008         1039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_197858_length_1039_cov_3.148374, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001008 DAOOLN010000000
VERSION     DAOOLN010001008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197858_length_1039_cov_3.148374"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..538)
                     /locus_tag="PLW80_11585"
     CDS             complement(68..538)
                     /locus_tag="PLW80_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67201.1"
                     /translation="MRLAIIALAALTLSSAEFVPIAEPPVVSIEREVKRPALSPYAWA
                     EAITGAPATILQATARTESGERDDAIGDGGVSLGRFQLNETFREWRVSRYGDYDPCDP
                     YSAAVIAGRIYVDNLARLGNPTLAIAAHRQGAGGVERNGPTRWYVERVYSNLRM"
     gene            complement(529..768)
                     /locus_tag="PLW80_11590"
     CDS             complement(529..768)
                     /locus_tag="PLW80_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67202.1"
                     /translation="MSEHKSGAVGRAFHIAVVERMGREIAALKAQRDELLAAAKAYQE
                     LSVCYRLNKRPTEKLFDRLETANFAIANVEATSCD"
     gene            complement(765..>1039)
                     /locus_tag="PLW80_11595"
     CDS             complement(765..>1039)
                     /locus_tag="PLW80_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67203.1"
                     /translation="DSSALREEVRQMTRSIDVILNTKTGDAVTVTGTWLPWIKATELE
                     PAEGGCFNDPRIMSGKYDVTTGFTNPGMAEWIIAEAERMLIEEAEE"
BASE COUNT          175 a          320 c          309 g          235 t
ORIGIN      
        1 cgcgcttttg ccgtaggcga aacgcggcta agcgtccgca taacatacgt tcgcaagttc
       61 ctgataacta cattcgcaaa ttgctataca ccctctcgac gtaccatcgc gtcgggccgt
      121 tgcgctcgac gcctccggcc ccttgccgat gcgccgcgat tgcgagcgta gggttgccga
      181 gcctggccag gttgtcgacg tagatgcggc cagcgatcac ggcggcacta taggggtcgc
      241 aagggtcata gtcgccgtag cggcttacgc gccattcgcg gaacgtctcg ttgagctgaa
      301 atctgcccag cgatacgccg ccgtcgccta tcgcgtcgtc gcgctcgccg gactcggtac
      361 gcgctgtcgc ctgtaggatc gtcgccggag cgccggttat cgcttcggcc caggcgtagg
      421 gcgatagggc cgggcgcttg acctctcgct cgattgagac cacgggcggc tcggctatcg
      481 ggacgaactc cgccgacgag agagtgagcg cggctagggc gatgatggct agtcgcatga
      541 cgtggcctcg acgttggcga tggcgaagtt ggctgtttca agcctatcaa ataatttttc
      601 tgttggtcgc ttgttcaaac gataacatac cgataactct tgataagctt tcgccgccgc
      661 cagcaactcg tcgcgctggg ccttgagggc ggcaatttct ctacccattc tttcgactac
      721 ggctatatga aaagctcgcc ctactgcacc tgatttatgc tcgctcattc ctcggcctcc
      781 tcgatcagca tccgctcggc ctcggctatg atccactcgg ccatgccggg gttggtaaag
      841 ccggtggtta cgtcgtactt gccggacatg atgcgcgggt cgttaaagca accgccctcg
      901 gctggctcta gctcggtcgc cttgatccac ggcagccagg tgccggtcac ggtcacggcg
      961 tcgccggttt tagtgttcag gattacgtct atactgcggg tcatttgccg tacctcctcg
     1021 cgtagcgcgc tagagtcgc
//
LOCUS       DAOOLN010001009         1038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_198140_length_1038_cov_4.244151, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001009 DAOOLN010000000
VERSION     DAOOLN010001009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198140_length_1038_cov_4.244151"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1038)
                     /locus_tag="PLW80_11600"
     CDS             complement(<1..>1038)
                     /locus_tag="PLW80_11600"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HPB67204.1"
                     /translation="STVVVEGQPLLELLAIGVEGQDGRDVLGGIIPAPKDPSVVDPPS
                     FDDSIVEERRENGDRLLKAAKSGELRFEKNALSIEAAQRVKGDVGPATGNIKFPGPVA
                     IAGTVLTGYAVVAGGDVTVTGAVEAALVSADGSVKIGEGVKGAKRGTIRARKGLEASF
                     AEQAMLLAVEDITLKNSALLCNVKTNGLLKLLGERGHLIGGICRARKGVEVQNLGSAN
                     GARTQVSFGQDYLVKDAIEAEEREIERVKALILQTDKRMRELEADGGSLDKVRQDKLR
                     LVKLLEQRSLRVFELREKFEEHFPSEIVVRGTVFAGVVIESHNRFHEVKQAKQKVAFS
                     FDPQLGRVVERP"
BASE COUNT          164 a          360 c          321 g          193 t
ORIGIN      
        1 ggccgctcga cgacgcggcc gagctggggg tcaaaggaaa aggccacctt ctgcttggcc
       61 tgcttgacct cgtgaaagcg gttatggctc tcgatgacca cccccgcgaa caccgtgccc
      121 cggacgacga tctcgctcgg gaagtgctcc tcgaattttt ccctgagctc gaagacccgg
      181 agcgatcgct gttcgagcaa cttgacgagc cggagcttat cctggcggac cttatcgagg
      241 ctcccgccgt cggcctcgag ctcgcgcatc cgcttatcgg tctggagaat cagcgccttg
      301 actctctcga tctcccgctc ctccgcctcg atcgcgtcct tgacgagata gtcctggcca
      361 aaggacacct gggtgcgcgc accgttggcc gagccgaggt tctgcacctc gactcccttc
      421 ctggcccggc agatcccgcc gatcaagtgg ccgcgctcgc cgagcaactt gaggaggccg
      481 ttggtcttga cgttacagag gagggcggaa ttcttaagcg taatgtcctc gacggccagg
      541 agcatggcct gctcggcgaa cgacgcctcg aggcccttgc gcgctcgtat cgtgccgcgc
      601 ttggcgcctt ttacgccctc gcctatttta accgagccat cggcggagac gagggccgcc
      661 tctacggcgc cggtcaccgt gacatcgccc ccggccacca cggcatagcc ggtcagcacc
      721 gtaccggcta tggccaccgg gccggggaac ttgatgttgc cggtggccgg cccgacgtcg
      781 cccttgacac gctgcgccgc ctcgatggac agggcgttct tctcgaagcg cagctcgccg
      841 ctcttagcgg ccttgagcag tcgatcgccg ttctcccttc gctcctcgac tatcgaatcg
      901 tcgaagctcg ggggatcgac aacgctcggg tccttggggg cggggatgat cccaccgagc
      961 acgtcgcggc cgtcctggcc ctcgacgccg atggccagga gctcgagcag aggctgcccc
     1021 tcgacgacga cggtagag
//
LOCUS       DAOOLN010001010         1037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_198583_length_1037_cov_2.180244, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001010 DAOOLN010000000
VERSION     DAOOLN010001010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198583_length_1037_cov_2.180244"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="PLW80_11605"
     CDS             <1..676
                     /locus_tag="PLW80_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HPB67205.1"
                     /translation="LLAIIIGIVDFSSVKEAAWIGIPKFTLPKFSAEGISIMLPIAIV
                     TMVEHFGDVLAIGNVVGKDFIKDPGIHRTLIGDGLATSLSALIGGPANTTYSENTGAV
                     ALTGVKNPIVMRIAACFAIVLAMIPKFTALIGTIPGPVIGGISILLFGMISSIGIKNM
                     VDARLNLGNSKLLIITATMLVLGLGGATFAVGNVKLSGLGLAAIFGIVLNLILRPKDV
                     TKADCD"
     gene            752..>1037
                     /locus_tag="PLW80_11610"
     CDS             752..>1037
                     /locus_tag="PLW80_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67206.1"
                     /translation="MTVGAIGCLAPGAPWSAPLAWVATKALALTHGDGAAVAIVGNIR
                     DMDARAILIADGFGDFRAAVKASADAVVSVRLDDVSATLVVDGVDLLAVPT"
BASE COUNT          172 a          350 c          325 g          190 t
ORIGIN      
        1 cctgctcgcg atcatcatcg gcatcgtcga cttcagctcc gtgaaggaag ccgcctggat
       61 aggcataccg aagttcacgc tgccgaagtt ctcggccgag ggcatctcga tcatgctgcc
      121 catagcgatc gtgacgatgg tcgagcactt cggcgacgtg ctcgcgatcg gcaacgttgt
      181 cggcaaggac ttcatcaagg atccgggcat ccaccgcacg ctgatcggcg acggcctcgc
      241 gacctcgcta tccgccctga tcggcggacc ggccaacacg acctactcgg agaataccgg
      301 cgccgtggcc ctgaccggcg tcaagaaccc gatcgtgatg cgcatcgccg cctgcttcgc
      361 gatcgtgctc gcgatgatcc cgaagttcac cgcgctgatc ggcaccatcc cgggcccggt
      421 catcggcggt atctcgatct tgctgttcgg catgatctcg tcgatcggca tcaagaacat
      481 ggtcgacgcc aggctcaacc tgggcaactc gaagctcctc atcatcacgg ccacgatgct
      541 cgtgcttggc ctcggcggcg ccacgttcgc cgtcggcaac gtcaagctat ccggcctcgg
      601 cctcgccgcg atcttcggca tcgtcctcaa cctgatactc aggcctaagg acgtcacgaa
      661 ggctgattgc gactgacgat tccatagagg gacgatgata cgggccgccc cttccggggg
      721 cggccctctt cttcaagcga ggagtgtgac gatgacggtg ggcgctatag gttgcctcgc
      781 gccaggggcg ccctggtcgg cgccgctggc ttgggtcgcg accaaggctc tcgccctgac
      841 gcacggcgac ggcgccgccg tggcgatagt cgggaacata cgcgacatgg acgctcgggc
      901 gatactgata gcggacggct tcggcgactt cagggccgcc gtcaaggcct cggccgacgc
      961 cgttgtctcg gtccggctcg acgacgtgtc cgcgacgctc gtggtcgacg gcgtcgacct
     1021 gctggccgtt ccgacgg
//
LOCUS       DAOOLN010001011         1036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_198811_length_1036_cov_2.884811, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001011 DAOOLN010000000
VERSION     DAOOLN010001011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198811_length_1036_cov_2.884811"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..288)
                     /gene="rpmG"
                     /locus_tag="PLW80_11615"
     CDS             complement(115..288)
                     /gene="rpmG"
                     /locus_tag="PLW80_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPB67207.1"
                     /translation="MAGKKQVVEIIALACTECKRRNYTTTKNRKTKQEKLELMKYCKW
                     DHKHTLHKETKVK"
     gene            complement(541..613)
                     /locus_tag="PLW80_11620"
     tRNA            complement(541..613)
                     /locus_tag="PLW80_11620"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(578..580),aa:Thr,seq:ggt)
     gene            complement(678..>1036)
                     /locus_tag="PLW80_11625"
     CDS             complement(678..>1036)
                     /locus_tag="PLW80_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPB67208.1"
                     /translation="GIALALSVASAVNTAALIVMMVRMGVDGLGEALGAVALYVVKLL
                     AYSAIAATPVLLLRRPLAEAFGGSSSGLVAYGAPFTLSALAFGAVGVGLLAVTKDRAA
                     GFLASSVTGRSKRRSN"
BASE COUNT          213 a          349 c          297 g          177 t
ORIGIN      
        1 agaccggcgc tctaccatta gagctactgg cctaatcgtc cgcctgccgg gctcgagggg
       61 agccgggcag gcgcacgacg cgatacggcc gagaccgcat cgccagacga aggcttactt
      121 taccttcgtc tccttgtgca gggtatgctt atgatcccac ttgcagtact tcatgagctc
      181 aagcttctcc tgcttggtct tcctgttctt cgtcgtcgta tagtttcggc gcttgcattc
      241 ggtgcatgca agggcgatga tctcaacgac ctgcttcttt ccagccatgg tccgaccctc
      301 ccggaagatt gacggccgcc tcggcgatcg tcgaaaaaaa atggagcccc ggtacgtcgc
      361 gacgcgccgc gtcaatcgcg ccgaccagcc ccagacacct acgaagagcc ctcgatcgga
      421 tgacgcgaag cgtcaaccga cttgcaggcc tggcgcatca gcgatgcgca gagagccctc
      481 gatcggacgg cgcggagcga cgaccgactt gcaggcctgg ttcgccaaag gcgaacagag
      541 agccctcgat cggaatcgaa ccgatgacct catccttacc atggatgtgc tctgccaact
      601 gagctacaag ggcgttataa gcgccggtac aataccaacc gtcgccgcct ttgtcaatag
      661 cgcggcgacg atcagtccta attggaacgc cttttgctcc tacccgtcac cgaggacgcg
      721 aggaagccag cggccctatc cttcgtcacg gcgagcaggc cgacgccgac cgctccgaac
      781 gcgagcgccg acagcgtgaa cggcgcaccg taggccacca agccggagct cgagccgccg
      841 aaggcctcgg caagcggcct ccgcagtagc aagactggcg tcgcggcgat cgcggaatag
      901 gccaatagct tcacgacata gagcgccacg gccccgagtg cctcgccgag accgtccacg
      961 cccatcctga ccatcatgac gatcagcgcg gccgtattca cggcgctcgc tacggacagg
     1021 gccaaagcta tgcccg
//
LOCUS       DAOOLN010001012         1036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_198886_length_1036_cov_2.339450, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001012 DAOOLN010000000
VERSION     DAOOLN010001012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198886_length_1036_cov_2.339450"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PLW80_11630"
     CDS             complement(<1..326)
                     /locus_tag="PLW80_11630"
                     /inference="COORDINATES: protein motif:HMM:NF018938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HPB67209.1"
                     /translation="MKKIRIGSGAGYAGDRLEPSLELIEHGALDYICYECLAERTIAI
                     AQQARLKDPSKGYNALLEYRMNAALPLAHKHGVKIITNMGAANPLAAVEVVKRIARDK
                     GISGIK"
     gene            complement(423..>1036)
                     /locus_tag="PLW80_11635"
     CDS             complement(423..>1036)
                     /locus_tag="PLW80_11635"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPB67210.1"
                     /translation="LFPVNIVLIVAAIAVLIQGKVPPHVVFMAAFCLALVINYPKVKD
                     QGARVDAHAKAALMMASILFAAGAFTGILKGTGMIKAMADLLVSVIPASLGARFPLIT
                     GVLAMPLSLVFDPDSFYFGVLPVLSSTAAEFGAQAVDVARAAILGQMTTGFPVSPLTG
                     STFLLIGLAGVDLGEHQKKTIPLAFATTLVMLLVAVVIGVIPL"
BASE COUNT          184 a          309 c          370 g          173 t
ORIGIN      
        1 atcttgatgc ccgagatgcc cttgtcccgc gctattcgct tgaccacctc gaccgccgcc
       61 agcggattgg cggcgcccat gttggtaatg atcttgacgc cgtgcttatg cgccagcgga
      121 agggcggcgt tcatgcggta ctcgagcagg gcgttgtagc ccttcgacgg gtccttgagc
      181 ctggcctgct gggctatggc tatggtgcgc tcggcgaggc actcgtagca gatgtagtcg
      241 agcgcgccgt gctcgatcag ctcgagcgac ggttcgagcc tgtcgccggc gtagccggcg
      301 ccgctcccga ttcggatctt cttcatggtg aatctcccgg ccccgtgggg cctgccctaa
      361 agaaaggaaa cgccggacgg agcggaggcg attgctccgc ccggcggcag tagcgacgga
      421 cgctagagag gaatgacgcc tatgactacg gcgacgagca gcatgaccag ggtcgtggcg
      481 aacgccaacg gaatggtctt cttctggtgc tcgccgaggt cgacgccggc gaggccgatg
      541 agcaggaacg tggagccggt cagggggctc accgggaagc cggtggtcat ctggccgagt
      601 atggcggcgc gggccacgtc cacagcctgg gccccgaact ccgcggccgt cgacgacagc
      661 accggaagga cgccgaagta gaaggaatcg gggtcgaaca cgagcgacag gggcatggcc
      721 agcacgccgg ttatgagcgg gaagcgggct ccgagcgaag ccgggatcac gctgacgagc
      781 aggtcggcca tggccttgat catgccggtg cccttgagta tgcccgtgaa ggcgccggcc
      841 gcgaagagga tgctcgccat catcagggcc gccttggcgt gggcgtcgac gcgcgcgccc
      901 tggtccttga ccttggggta gtttatgacg agggccaggc agaacgccgc catgaatacg
      961 acgtgcggcg gtaccttgcc ctggatcagt accgctatcg cggcgactat aagcacgatg
     1021 ttgacgggga agagtc
//
LOCUS       DAOOLN010001013         1033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_199893_length_1033_cov_2.103272, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001013 DAOOLN010000000
VERSION     DAOOLN010001013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199893_length_1033_cov_2.103272"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..135)
                     /locus_tag="PLW80_11640"
     CDS             complement(<1..135)
                     /locus_tag="PLW80_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB67211.1"
                     /translation="MRAVDGLSFEARPGEIFGLIGPNGAGKSTTIRMIMNVIAPDEGT
                     I"
     gene            537..941
                     /locus_tag="PLW80_11645"
     CDS             537..941
                     /locus_tag="PLW80_11645"
                     /inference="COORDINATES: protein motif:HMM:NF013253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HPB67212.1"
                     /translation="MTYNEVRAKAKQTIGPYCKVCPECNGQACKGMIPGPGGKGTGSG
                     FIRNYARLRDIRLAMDTVYEPRTVSTATNLFGQDFELPVFAAPVGAVGLHYSDAYTDL
                     SYSAAVLEGCKAAGSAAFTGDGVKDEVYRELA"
BASE COUNT          206 a          336 c          288 g          203 t
ORIGIN      
        1 tatcgtcccc tcgtccgggg cgatcacgtt catgatcatg cgtatcgtcg tcgatttgcc
       61 agctccgttc gggcctatca gtccgaagat ttctcccggc cgagcctcga acgacagccc
      121 gtcgacggcg cgcaccgtct cgtaggtctt tacgaccttc tctacgctga gcacggttca
      181 ctccttgttc gcccgcgcgg cgatccgccg cgggcgcatg agataaccgt agtataactg
      241 caaattcctc gatagcatac ttcgttctcg cttgccgcag cccgctccag gtcctatact
      301 cgcttccgtg ctgacaatgc gtacaggcgc gaagtcgcgg cccgcactgc ttatggtgtg
      361 aggaaattga agtgttacaa accttgtgta tccaaggagc gtccagctgc ttgcgactac
      421 gaggtattgg ttctacagtc tcggatggcg atctgcgcgg ctaactcgtt cacaaacgct
      481 atgttacgca caacggatac ataattttcg aaccactacc caaggagacc atcgacatga
      541 cgtacaacga ggtgcgggcc aaggctaagc agaccatcgg accgtattgc aaggtatgcc
      601 cggagtgcaa tggacaggcg tgcaagggca tgatacccgg ccccggcggc aagggtaccg
      661 gcagcggctt catacgcaac tacgcccgcc tgcgggatat caggctcgcc atggacaccg
      721 tctatgagcc gcgcaccgtc tcgacggcca cgaacctctt cggccaggac ttcgagctac
      781 cggtcttcgc agcgccggtc ggggcggtcg gcctgcacta cagcgacgcc tataccgacc
      841 tgagctactc cgcggccgtg ctcgagggct gtaaagccgc cggcagcgcg gccttcaccg
      901 gagacggcgt caaggacgag gtttaccgcg aactcgccta gctcggcgtg cccatcctct
      961 cggacgaaat ctaccacggc ctcatctacg gcgacgcaaa ggcccattcg gcgctcgaat
     1021 tcaccgacca ggc
//
LOCUS       DAOOLN010001014         1033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_199907_length_1033_cov_2.013292, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001014 DAOOLN010000000
VERSION     DAOOLN010001014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199907_length_1033_cov_2.013292"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..665
                     /locus_tag="PLW80_11650"
     CDS             60..665
                     /locus_tag="PLW80_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB67213.1"
                     /translation="MGRSGRAPTTRDLKARDTRERIYESAWSLIVEEGFDKVSVDRIC
                     AGAGVAKGSFYHHFRSKGDLIVEGYAMCDAYFDGEVAGRLEAPDAPGRIVEFVAHQMR
                     YAVRTGIDMMRQVYKSQLENGTRFFVSSERSLPRILRGIVVDGQAAGELRADLAADYV
                     VSFVLRFSRGTIYDWCLRDGSYDLEAVATEACARLVAVFKP"
     gene            complement(662..>1033)
                     /locus_tag="PLW80_11655"
     CDS             complement(662..>1033)
                     /locus_tag="PLW80_11655"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPB67214.1"
                     /translation="VPAALLRERGYSVTAYDPYFAPGELWRSRSWDAILVHEVAEHLA
                     EPGETLAELAALLRPGGAWCIRTRFPPKDRESFARWRYRMDSTHVGFFGPGSFTWLSA
                     RLGLEIALVEPPDRAVLVRPA"
BASE COUNT          167 a          335 c          375 g          156 t
ORIGIN      
        1 tgagtgtagc ctcgtttctg ccatagcgca agcgccggaa tgtcgggtac agtgatcgca
       61 tgggaagatc aggccgggct ccgacgacga gggatctcaa ggcgcgggat acgcgcgagc
      121 gcatctacga gtcggcgtgg agcctcatcg tcgaggaggg cttcgacaag gtgtcggtgg
      181 accgcatctg cgccggggcc ggcgtcgcga agggctcgtt ctaccaccac ttcaggagca
      241 agggcgacct gatcgtcgag ggctacgcca tgtgcgacgc ctacttcgac ggcgaggtgg
      301 ccggccgact cgaggcgccg gacgcacccg gccgcatcgt cgagttcgtg gcccatcaga
      361 tgcgctacgc cgtccggacc ggcatcgaca tgatgcgcca ggtctataag agccagctcg
      421 agaacggcac gcgcttcttc gtatcgtcgg agcggtccct gccgaggata ctccggggca
      481 tcgtggtaga cggtcaggcc gcaggcgagc tgcgggccga cctggccgct gactacgtgg
      541 tctccttcgt gctgcgcttc tcgcggggca ccatctacga ttggtgcctg cgcgacggct
      601 cctacgacct cgaggcggtg gccaccgagg cctgcgcccg gctcgtcgcc gtcttcaagc
      661 cttaggccgg gcgcacgagt accgctcggt cgggcggctc gacgagggct atctcgaggc
      721 cgaggcgcgc ggacagccag gtgaatgagc cggggccgaa gaacccgaca tgggtcgagt
      781 ccatccgata gcgccagcgc gcgaacgact cgcggtcctt gggcggaaac cgggtgcgta
      841 tgcaccaggc gccgcccggg cggagtaggg ccgcgagctc ggccagcgtc tccccgggct
      901 ccgcgaggtg ttcggcgacc tcgtgtacca ggatggcgtc ccaggaccgc gaacgccaga
      961 gctcgcccgg ggcaaagtac gggtcgtagg cggtcaccga gtagccccgc tcgcggagca
     1021 gggcggccgg cac
//
LOCUS       DAOOLN010001015         1032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_200091_length_1032_cov_3.021494, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001015 DAOOLN010000000
VERSION     DAOOLN010001015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200091_length_1032_cov_3.021494"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PLW80_11660"
     CDS             <1..429
                     /locus_tag="PLW80_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPB67215.1"
                     /translation="IDLYINSPGGSVTAGLAIYDTMQFVKPDVKTICVGQASSMGALL
                     LAGGAAGKRLALPSSRILIHQPWGGVQGQASDISIQAREIIRMKKMTVEYFAKHTGKS
                     VDRVAQDMERDFFMSAQEAVEYGLVDSILERRKHGALEGK"
     gene            407..>1032
                     /locus_tag="PLW80_11665"
     CDS             407..>1032
                     /locus_tag="PLW80_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB67216.1"
                     /translation="MARSKASKGSDGEDLHECSFCGKSAEFAKRLIAGPGVYICDECV
                     QVCNRILAEDESAASGDFSTDVPKPKDIKDYLDQFVIGQDYAKKVLSVAVYNHYKRIA
                     HQHSLTDGIELEKSNVLLLGPTGTGKTLLARTLAKKLNVPFAIADATTLTEAGYVGED
                     VENILLKLIQNAGGNVSLAEKGIIYIDEIDKIARKTQNVSITRDVSGEG"
BASE COUNT          220 a          327 c          319 g          166 t
ORIGIN      
        1 atcgacctat atatcaattc ccccggcggc tcggtgacgg ccgggctggc catctacgac
       61 acgatgcagt tcgtcaagcc tgacgtcaag accatctgcg tcggacaggc gtcgagcatg
      121 ggcgccctgc tgctcgcagg aggggcggcc ggcaagcgcc tggccctccc gagctccagg
      181 atcctgatac accagccctg gggcggagtc caggggcagg cgtccgatat cagcatccag
      241 gctcgagaga tcatccgcat gaagaagatg accgtcgagt acttcgcgaa gcacaccggc
      301 aagagcgtcg accgcgtggc ccaggacatg gagcgcgact tcttcatgtc ggcgcaggag
      361 gcggtcgagt acggcctggt cgactcgatt ctcgagcgga ggaagcatgg cgcgctcgaa
      421 ggcaagtaag ggctccgatg gcgaggacct ccacgagtgc tcgttctgcg gcaaatcggc
      481 cgaattcgcc aagcgcctga tagccggccc cggcgtctat atctgcgacg agtgcgttca
      541 ggtctgcaac cgcatcctcg ccgaggacga gagcgccgcc tcgggcgact tctcgacaga
      601 tgtgcccaag cccaaggaca tcaaggacta cctggaccag tttgtcatcg gccaggacta
      661 cgccaagaag gtgctgtcgg tagcggtcta caaccactat aagcgcattg cccatcagca
      721 cagcctgacc gacggcatcg agctcgagaa gtcgaacgtg cttctgcttg gtcccaccgg
      781 caccggcaag acgctcctcg cgcgcacgct ggccaagaag ctgaacgtgc ccttcgccat
      841 cgccgacgcc acgacgctga ccgaggcagg ctatgtcggc gaggacgtgg agaacattct
      901 ccttaagctg atccagaacg ccggcggcaa cgtatcgctc gccgagaagg gcatcatcta
      961 catcgacgag atcgataaga tagcccgtaa gacgcagaac gtgtcgatca cccgcgacgt
     1021 ctctggcgag gg
//
LOCUS       DAOOLN010001016         1031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_200550_length_1031_cov_2.184426, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001016 DAOOLN010000000
VERSION     DAOOLN010001016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200550_length_1031_cov_2.184426"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..701)
                     /locus_tag="PLW80_11670"
     CDS             complement(156..701)
                     /locus_tag="PLW80_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPB67217.1"
                     /translation="MEIQKDRVVSIDYMLKDDQGQIIDRSDGEPLTYLHGNDNLIPGL
                     ERELHGKVAGDRISCVIAPADAYGELDEEMIFKVAKSDFAEPEKIEVGMQFQAQDASG
                     AHIVTVVGIEGDEVKIDANHPLAGKSLHFDVTVKDVREASPEELEHGHVHAESGCGCG
                     DGCCGDGDCDDEGGCGCGGCH"
BASE COUNT          171 a          329 c          329 g          202 t
ORIGIN      
        1 gccgcccgag gtgatgacca ggacggacga tggcggcccc ccgtagacgg agaccgccag
       61 gcatacgaac agggccgccg agagcgccac gatcagggcg tcaagccacc tgaccttgac
      121 ccccccgcgg ccccctcgcg cgccggcgcg tcggatcagt ggcatccgcc gcagccgcat
      181 ccgccttcgt cgtcgcagtc gccgtcgccg cagcatccgt cgccgcagcc gcagccgctc
      241 tcggcgtgca cgtggccgtg ctcgagctcc tcggggctgg cctcgcgcac gtccttgacg
      301 gtcacgtcga agtgcagcga cttgccggcg agcgggtggt tggcgtcgat cttgacctcg
      361 tcgccctcga tgcccaccac cgtgacgatg tgggcgccgc tggcgtcctg ggcctggaac
      421 tgcatgccga cctcgatctt ctcgggctcg gcgaaatcgc tcttggcgac cttgaagatc
      481 atctcctcgt cgagctcgcc gtaggcgtcg gccggggcga tgacgcagct gatcctgtcg
      541 ccggcgacct tgccgtgcag ctcgcgctcg aggccgggga tcaggttgtc gttgccgtgg
      601 aggtaggtga gcggctcgcc gtcggaacgg tcgatgatct gtccctggtc gtccttgagc
      661 atgtagtcta tcgagacgac gcgatccttc tggatttcca tgtagccatc cttacaaaga
      721 gagagtgtgt atcgacgggc ggggccgaac ggaacgatgc gaaccatgat gcgtctagag
      781 cggctaagcc ctgcctggaa gcccaggata tcacgtccgt aaagaaacgc cttttcgtct
      841 agcatagggt acttattgga atggagattt tcaagtaccg taccctacat acagggcgga
      901 tggcgttgaa ggtaggtcgc tatggtgcta tagtccgctc ccggagggcg acatgaccat
      961 catccaggcc gctattcttg ggcttttcca gggaatcgct gaattccttc ccatttcgtc
     1021 gagcgggcat c
//
LOCUS       DAOOLN010001017         1031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_200587_length_1031_cov_1.949795, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001017 DAOOLN010000000
VERSION     DAOOLN010001017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200587_length_1031_cov_1.949795"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLW80_11675"
     CDS             <1..444
                     /locus_tag="PLW80_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67218.1"
                     /translation="RISGPRDQVDEAAARLELIADTYLSAGAPVMNALPSLLPGVDDF
                     VGSVLGRLRSNLSEARTVFGGDGSPYRVLRCDGGWTALLEFPRYQGEESVILGLLEHA
                     SVAIQPGYFFDMEREGSLALSLILEPEVFAEGVRRIKAYIDSMGA"
     gene            533..>1031
                     /locus_tag="PLW80_11680"
     CDS             533..>1031
                     /locus_tag="PLW80_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HPB67219.1"
                     /translation="MAAKLLDGKRLAEEIRVGLGPRVLALAERGVVPGLAVLLVGDDP
                     ASVSYVTAKEKACAELGMRSVERRLPAEAGEAAIIEVVREFNDDPSVHGILIQLPLPK
                     GVSEAKVVEAISPEKDVDGFTPVNVGRMVIGEPCFLPCTPHGVLKLIEVSGIRTAGAH
                     AVVAPK"
BASE COUNT          178 a          312 c          356 g          185 t
ORIGIN      
        1 cggatttccg gcccgcgaga ccaggtcgac gaggcggctg cgaggctcga gctgatcgcc
       61 gatacctacc tgtccgccgg ggcgcccgtg atgaacgcgc taccgagcct gctacccgga
      121 gtcgatgatt tcgtaggttc ggtcctcggg cgtctgcgct caaacctaag cgaggcgcga
      181 acggtattcg gaggcgacgg atctccgtac cgggtactgc gctgcgacgg cggatggacg
      241 gccctcctcg aattcccacg ctaccaaggc gaggagagcg tgatccttgg cctcctcgag
      301 catgcgtcgg tagcaatcca gccgggatac ttcttcgata tggaacgaga gggctctcta
      361 gccctaagtc tgatcctcga acccgaggtc ttcgccgaag gggtccgcag gatcaaggcc
      421 tacatagact cgatgggagc gtgacgggtt ctcagccgag gattcggtcc aaggcttgtc
      481 ctgaccgcgc gtggggtagt atcgtgtagc tgtggatcat caaggaggcg acatggccgc
      541 gaagctgttg gacggcaaga ggctggcgga ggagatccgc gtcgggctcg ggccgagggt
      601 gctcgccctg gcggagcggg gcgtggtccc tggcttggcc gtactattgg taggtgacga
      661 cccggccagc gtgtcgtacg tcacggccaa ggagaaggct tgcgccgagc tcggcatgag
      721 gagcgtcgag cgccgtttgc ctgccgaggc cggcgaggcc gctatcatcg aggtagtgcg
      781 cgaattcaat gacgacccat cggtacacgg catactgatc cagttgccgc tccccaaggg
      841 cgtctccgag gctaaggtgg ttgaagccat ctccccggag aaggatgtcg acgggttcac
      901 gccggtcaac gtcggccgca tggtcatagg cgagccgtgt ttcctgccgt gcacccccca
      961 cggcgtgctc aagctgatcg aggtctcggg catccgtacc gccggcgccc acgccgtggt
     1021 cgcgcccaag g
//
LOCUS       DAOOLN010001018         1029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_201059_length_1029_cov_3.630390, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001018 DAOOLN010000000
VERSION     DAOOLN010001018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201059_length_1029_cov_3.630390"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..>1029)
                     /locus_tag="PLW80_11685"
     CDS             complement(93..>1029)
                     /locus_tag="PLW80_11685"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB67220.1"
                     /translation="RPLLGLAKADILAYLDAEGLDYRVDSTNDSDAYLRNRVRHRLVP
                     AAMDVFPSLRASLATVAEKASLDEEALSALADALFGLGDDGARADWLEAGRYDAAPEA
                     VRIRALYSLAMPLFEGAGATRLPWRLVRAAASSARREGVLASGAGVLFSRNGGRIEAR
                     QALGPHLSRLSAPAGADRADSGFSLIANGPGSYRIGKGGGCTIYSCGQAPGLRADAFS
                     WPLCVRSRRPGDRLATRGGAKRVDAILSELGLSGASREATPVVEDALGLVAVLASSVG
                     LCDVYRRNDALVGEPVGGYLVVELKGVMYIDAVRR"
BASE COUNT          183 a          353 c          343 g          150 t
ORIGIN      
        1 aggccgcgag caaggcaatg aatagtataa gggagacgcg agcattgcga ggcggcctcg
       61 catccggctt attcggcgtc ctcggcgtat tcttatcgtc gaactgcatc gatgtacatg
      121 actcctttca actccactac caggtagccg ccgaccggtt caccgaccag ggcatcattc
      181 cgcctataga cgtcgcacag gccaacactc gaggcgagga cggcgaccag gcctaaggcg
      241 tcctccacga cgggcgtagc ctcgcgcgaa gccccgctca agccgagctc ggataggatg
      301 gcgtcgaccc tcttggcgcc gccccgcgtg gctagacgat cgccgggccg cctggagcgg
      361 acgcacagcg gccacgagaa ggcgtccgcg cgcaatccag gcgcttgccc gcacgagtat
      421 atcgtacagc cgccgccttt gcctattcga tacgatccgg gaccgttggc gatgaggctg
      481 aagccggagt cggccctgtc ggcaccggcc ggggcggaca ggcgcgacag gtgcgggccg
      541 agggcctgtc gggcctcgat cctgccgccg ttccgcgaga acaggacgcc ggccccggaa
      601 gccagcaccc cttcgcgcct agcggacgac gcggccgccc ggacgagccg ccacggcagt
      661 ctcgtcgctc ctgcgccctc gaacagcggc atggccagcg agtagagcgc tcgtatcctg
      721 acggcctccg gcgcggcgtc gtagcgaccc gcttcgagcc aatcggcgcg ggcgccatcg
      781 tctcctaacc cgaacaaagc gtcggccaga gccgagaggg cttcctcatc gaggctggcc
      841 ttctcggcga ccgtagccaa cgacgcgcgc agcgacggga agacgtccat cgcagcgggc
      901 acgagcctat gcctgacccg gttgcgcagg tatgcatcgg agtcgttggt cgagtcgacg
      961 cggtaatcca atccctcggc gtcgaggtac gcaaggatgt cggccttggc caggccgagg
     1021 agggggcgg
//
LOCUS       DAOOLN010001019         1028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_201377_length_1028_cov_3.228160, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001019 DAOOLN010000000
VERSION     DAOOLN010001019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201377_length_1028_cov_3.228160"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PLW80_11690"
     CDS             complement(<1..643)
                     /locus_tag="PLW80_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67221.1"
                     /translation="MSNRPGHPSSPLQTIAYALLAAAIGLAFTAGVYAELAADRRDRL
                     EAGFAFDAAQRTELVREAFAHHGDALESIKRFFENSSGVTNGEFTRFASAWASVKGFR
                     SLVWAVPDDGSDGNPPGGGELRPVYRVPTLDSDAELAWLGLSAVRSAVAEARVSGRMN
                     ASELFELGDARYDPFVALCLPVYAAWDESGVRRFGLVIGLVSLPTVSNALISPE"
     gene            801..>1028
                     /locus_tag="PLW80_11695"
     CDS             801..>1028
                     /locus_tag="PLW80_11695"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPB67222.1"
                     /translation="MNRITEHPVIDAGQPRPEVRFSFDGEPLAGYDGEAVSSALFAAG
                     VKRFSEHRKDGAPQGIFCANGQCSQCNVLVDG"
BASE COUNT          194 a          342 c          343 g          149 t
ORIGIN      
        1 gctcggggga gatcagcgca ttcgaaaccg tgggcaggct gaccagcccg atgacaaggc
       61 cgaagcgccg gacacccgac tcgtcccacg cggcgtagac cggtaggcag agcgcgacga
      121 agggatcgta ccgggcgtcg ccgagctcga acagctccga ggcgttcatg cggccgctga
      181 cgcgggcctc ggcgaccgcg gacctgaccg ccgacaggcc gagccaggcc agctcggcgt
      241 ccgagtcgag ggtgggcacg cggtagacgg gcctgagctc gccgccgccg ggcggattcc
      301 cgtcggaccc atcgtccggc accgcccaga cgagcgagcg gaagcctttg accgaggccc
      361 aggcggacgc gaaacgcgtg aactcgccgt tcgtcacgcc ggaggaattc tcgaaaaagc
      421 gcttgatcga ctcgagggcg tcgccgtggt gggcgaaggc ctccctgacc agctcggtcc
      481 gctgcgcggc gtcgaaggcg aagccggcct cgagccggtc gcgccggtcg gccgcgagct
      541 cggcatagac gcccgccgtg aaggccaagc cgatggcggc ggcgagcagc gcgtaggcta
      601 tcgtctgcaa tgggctcgaa ggatgtccgg gtcggttgct catcgcccct actataatcg
      661 atgaaacgta gaggcgctat atagaccatg tatccgcgca cgggtttcat aaaataaaac
      721 ccaggtgcgt atactgaaat ttctgtattg acagttcgat tccgacgtgg ttccatatcc
      781 gcgcatcgga gacaaccacc atgaaccgaa tcaccgaaca tccggtaatc gacgccgggc
      841 aaccgcggcc cgaggtccgc ttctccttcg acggcgagcc gctcgccggc tacgacggcg
      901 aggccgtcag ctccgccctg ttcgccgccg gcgtcaagcg cttctcggag caccgcaagg
      961 acggagcgcc gcagggcatc ttctgcgcca acggccaatg ctcgcagtgc aacgtgctcg
     1021 tcgacggc
//
LOCUS       DAOOLN010001020         1026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_201986_length_1026_cov_3.138002, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001020 DAOOLN010000000
VERSION     DAOOLN010001020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201986_length_1026_cov_3.138002"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="PLW80_11700"
     CDS             complement(<1..880)
                     /locus_tag="PLW80_11700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HPB67223.1"
                     /translation="MRLRRSIYAPIAFFLAAAGASAQVPAKSAEPLKVASAISAVTVF
                     SDRALVRRTAELRVQAGETTLVFTDLPAAIDPASLQVSGSGLFTLRDVRVTTRQLSRD
                     VSAERKALEDERRAYEASIAIESDRVREAEAERTFVDNMAKRVTSAASEDEKAPLDTA
                     AWARMLDFYRGRYQALNLAVREAKASITTLQAEVDRVNRELRALGGGSGLSVVEAELS
                     LEAQAPTTARLVLSYLVSGPSWMPDYVVRADSAGGKLSLHYRATVRQGTGEDWSGVAL
                     SLSTARPQVGGSAPELE"
BASE COUNT          168 a          345 c          330 g          183 t
ORIGIN      
        1 gctcgagctc gggcgccgag ccgcccacct ggggcctggc cgtcgaaagg ctgagcgcca
       61 cgcccgacca gtcctcaccg gtgccttgcc tgaccgtcgc ccgatagtgg agcgacagct
      121 tgccgccagc cgaatcggct cgcacgacgt aatcgggcat ccaggacggc cccgagacta
      181 ggtatgacag tacgagccga gccgtcgtag gcgcctgcgc ctcgagcgac agctccgcct
      241 cgacgacgga aaggcccgag ccaccgccga gcgcccgcag ctcgcggttc acccggtcga
      301 cctcggcctg cagggtggtt atgctcgcct tggcctcgcg taccgccagg ttcagggctt
      361 ggtagcggcc acgatagaag tcgagcatcc tggcccaggc cgccgtatcg agcggcgcct
      421 tctcgtcttc gctcgccgcg ctcgtgacgc gcttggccat attgtcgacg aacgttcgtt
      481 cggcctccgc ctcgcgcacg cggtccgact cgatcgctat cgaggcctcg taggctcgac
      541 gctcgtcctc gagcgccttg cgctcggcgg aaacgtcgcg agacagctgc ctcgtagtta
      601 cccgtacgtc gcgtagcgtg aacaaaccgg agccgctcac ctgaaggctg gcggggtcaa
      661 tcgccgcagg caggtcggtg aagacgagcg tcgtctcacc ggcctgaacg cgtagttcgg
      721 ctgtccgcct gacgagcgcg cggtccgaga acacggtaac ggcgcttatg gccgaggcga
      781 ccttgagcgg ctcggcggac ttggcgggta cctgggcgct cgccccggcc gcagccagga
      841 agaacgcgat cggggcgtat atgctcctac gtagtctcac ggatagacct ccattcgata
      901 cctctagtgt aacgcgtcgg acaggttttc tgtagtgcgg gctcgccgtc ggcttggtcg
      961 taccagctga cagtattgta atgaatgtct tgcaagtata tgatcttaac actccagggc
     1021 tcccgg
//
LOCUS       DAOOLN010001021         1024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_202658_length_1024_cov_3.049536, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001021 DAOOLN010000000
VERSION     DAOOLN010001021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202658_length_1024_cov_3.049536"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PLW80_11705"
     CDS             <1..254
                     /locus_tag="PLW80_11705"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HPB67224.1"
                     /translation="FRFHDRIVKGAGNVLFRSMQNTSRELHLFYTRRFYSLPGALEAT
                     AAQQLAIAKAIGSGDPEASAAAMMTALEYGRRAIERMLG"
     gene            395..>1024
                     /locus_tag="PLW80_11710"
     CDS             395..>1024
                     /locus_tag="PLW80_11710"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HPB67225.1"
                     /translation="MRSIRTKIVTLAAAAALVVAVCLSIVFINLLRSSVDEQVAALET
                     TLRDDFDLLIKSEIETAVSMLERLAALRDEGSLGDAEARNLAATLLRELRYGEDGYFW
                     ADTPQGDNVVLLGGTSEGTNRMALKDVNGYELVRAIIDAAMKGGGYTDYWFPRAGETE
                     AAPKRGYSKHAPGFNWVIGTGNYTDDIDQVITAKREAAAASLRRALVLIV"
BASE COUNT          191 a          346 c          312 g          175 t
ORIGIN      
        1 atttccgttt ccacgaccgc atcgtcaagg gcgcgggcaa cgtactgttc aggtcgatgc
       61 agaacaccag ccgcgagcta cacctgttct acacccggcg cttctactcg ctgccgggcg
      121 ctctcgaggc cacggccgcc caacagctcg ccatcgcgaa ggccataggc tcgggcgacc
      181 ccgaagcatc cgccgccgcg atgatgaccg ccctcgagta cggccgccgc gcgatcgaac
      241 gcatgctcgg atagcgcacg ctcggatagc gcacatgcgg ataccacatc gctaagctcg
      301 cgatggagcg ggccaggccc ggttccttgc gctcgatcga ttcatacata cacttaccat
      361 atatgagctt atcgatcacc aggagtccgt gcttatgaga tctatccgaa ccaagatcgt
      421 gacgctcgcc gcggctgcgg cgctcgtcgt cgcggtctgt ctgtcgatcg tattcatcaa
      481 cctgctccgc tcgtcggtcg acgagcaggt cgccgcgctc gagacgacgc ttcgcgacga
      541 cttcgacctg ctcatcaaga gcgagatcga gacggcggtc tccatgctcg agcgcttagc
      601 ggcgcttcgc gacgaaggct cgcttggcga cgccgaggct cggaatctgg cggctacgct
      661 cctccgcgag cttagatacg gcgaagatgg gtatttctgg gcggacacgc cccagggcga
      721 caacgtcgtg ctcctcggcg gcacgtccga gggtacgaac cgcatggctc tcaaggacgt
      781 caacggttac gagctcgtca gggccatcat cgacgcggcc atgaagggcg gcgggtacac
      841 cgactattgg ttcccccgcg caggcgagac ggaggcggct ccgaagcgcg gctacagtaa
      901 gcacgcgccg ggtttcaact gggtgatcgg caccggcaac tacacggacg acatcgacca
      961 ggtgataacc gccaagcgcg aagccgcggc cgcgtcgctc cggcgcgccc tcgtcctcat
     1021 cgtc
//
LOCUS       DAOOLN010001022         1023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_203008_length_1023_cov_2.981405, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001022 DAOOLN010000000
VERSION     DAOOLN010001022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203008_length_1023_cov_2.981405"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..412)
                     /locus_tag="PLW80_11715"
     CDS             complement(14..412)
                     /locus_tag="PLW80_11715"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPB67226.1"
                     /translation="MPKQSSIRNAADVVTVLGGSTSFSGTLKFDTSLMIRGSFDGDID
                     AKGALYIDEGATVNVGRIRAMSIVVAGSVRGDIEAVDKVELRSSAQVRGNVKTARLRI
                     ADGVLFEGRCEMVRDPEAFDPFAAKAPAES"
     gene            complement(428..649)
                     /locus_tag="PLW80_11720"
     CDS             complement(428..649)
                     /locus_tag="PLW80_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB67227.1"
                     /translation="MISEDKRRVLDLFAEGRKLYKLMRFSDAADKFDEALKIDPTDGP
                     SKVYLDRCDHYVIDPPDEDWDGVFVMKHK"
     gene            complement(675..>1023)
                     /locus_tag="PLW80_11725"
     CDS             complement(675..>1023)
                     /locus_tag="PLW80_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67228.1"
                     /translation="YITKPFNFAEVLARIRVILRSYELVKQIDNREQSIIVGERAVAE
                     MQKAIASTKRSLEQIGSASDLVTAKELAAKTRAAPVELDSRFGSFSSEYAELRNKASD
                     IKTLRGKPHRAES"
BASE COUNT          159 a          331 c          296 g          237 t
ORIGIN      
        1 gccttccgcc atctcaggat tcggccgggg ccttggccgc gaacgggtcg aacgcctcgg
       61 ggtcacggac catctcgcaa cggccctcga acagcacgcc atcggctatc ctgagcctag
      121 ccgtcttgac gttgccgcgt acctgggcgc tcgagcgtag ttccaccttg tcgacggctt
      181 ctatgtcgcc gcggacggag cctgcgacga cgatgctcat cgcccgtatc ctcccgacgt
      241 tcaccgtcgc gccctcgtcg atatagaggg cgcccttcgc gtcgatgtcg ccgtcgaagc
      301 taccgcggat catgaggctc gtgtcgaatt ttagcgttcc ggagaagctc gtcgagcctc
      361 cgagtacggt cactacgtcc gccgcgttgc ggatcgatga ttgttttggc atccaggcct
      421 cggctcccta tttgtgcttc atgacgaaga cgccgtccca atcctcgtcg ggcggatcga
      481 tcacgtaatg gtcgcatcgg tcgagataca ccttcgacgg accgtcggtc gggtcgatct
      541 tgagcgcttc gtcgaattta tctgccgcgt cggagaagcg catcagctta tacaacttcc
      601 tgccctcggc gaacaggtcg aggacgcgtc tcttgtcttc gctgatcata ccggctcctt
      661 ccgccccgcc gtcgctacga ctcggcgcga tggggcttgc ctcgtagcgt cttgatatcg
      721 ctcgccttgt tgcggagctc tgcgtactcg gaggagaacg agccgaacct ggagtcgagc
      781 tccactggcg cggccctcgt cttggccgcc agctccttag ctgtaaccag gtccgacgcg
      841 gaccctatct gctcgagcga tcgtttcgtc gaggctatcg ccttctgcat ctcggctacg
      901 gccctctcgc cgacgatgat gctctgttcc cggttatcta tttgttttac gagttcgtac
      961 gagcgcagga tgacccggat tctggccagg acctcggcga agttgaacgg ctttgtaatg
     1021 tag
//
LOCUS       DAOOLN010001023         1022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_203438_length_1022_cov_2.352637, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001023 DAOOLN010000000
VERSION     DAOOLN010001023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203438_length_1022_cov_2.352637"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="PLW80_11730"
     CDS             <1..899
                     /locus_tag="PLW80_11730"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HPB67229.1"
                     /translation="AFVFAAQMVNFTIPGTGSSGHLGGGLFLAALLGPYAGFLAMGSV
                     LAIQALCFADGGLLAYGCNVFNLGFFTCFLAYPLIYQPIAGDGSSKTKSFIAAMVAAV
                     VGLQAGAFGVVIETMLSGKTELPFGAFVALMQPIHLAIGVVEGFVTAAVLAYVRSASP
                     ELLAPGATVAVSSARPKALLGLILATLVVGIGLSWFASTDPDGLEWSMEKVSGVAELE
                     VEGGVHETAAGIQAVTAFLPNYGFKSAETEEAEPSWPAIDAGTSLSGVVGGVLALGLS
                     ALAGLAAFGLRRKPRPAGTGKA"
BASE COUNT          140 a          379 c          311 g          192 t
ORIGIN      
        1 ccgccttcgt cttcgccgcc cagatggtca atttcaccat ccccggcacg ggctcgagcg
       61 gacacctcgg cggcggcctg ttcttggccg cgctactcgg cccctatgcg ggcttcctgg
      121 ccatggggtc ggtcctggcc atccaggccc tctgcttcgc cgacggcggc ctcctggcct
      181 acggctgcaa cgtattcaac ctcggtttct tcacctgctt cctcgcctac cccctcatct
      241 accagcccat agccggtgac ggttcctcca agaccaagtc attcatagcg gccatggttg
      301 ccgccgtcgt cggcctccag gccggcgcct tcggcgtggt tatcgagacg atgctgtccg
      361 gaaagacgga gctacccttc ggggccttcg tcgccctgat gcagcccata cacctggcca
      421 taggcgtcgt ggagggtttc gtgaccgccg ccgtcctggc ctacgtccgc tcggcctcgc
      481 ccgaactcct ggctccgggc gccaccgtag ccgtatcctc cgcccgccca aaggcgctcc
      541 tggggctcat tctagcgacc ctggtcgtcg gcatcggcct gtcctggttc gcctccaccg
      601 atcctgacgg gctcgagtgg tctatggaga aggtctcagg tgtggccgag ctcgaggtcg
      661 agggcggagt gcacgagacg gccgccggca tccaggccgt taccgccttc ttgcccaact
      721 acggcttcaa atcggccgag accgaggaag cggagccgtc ctggccggcc atcgacgctg
      781 gcacgagcct gtccggcgtc gtcggtggcg tcctggcgct cggcttgtct gccctcgccg
      841 gcctggcagc gttcggcctg cgccgcaagc cacgcccggc cggaacagga aaagcttgaa
      901 gatgagagac ccgtcgcgga ccctggccgc gttctacacc gttgacgagc tatcgcggcg
      961 acatagcccg gtccacgaca ggcatcccct ggccctctgc gcgacgacgg ccgccttcat
     1021 gg
//
LOCUS       DAOOLN010001024         1021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_203661_length_1021_cov_3.292961, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001024 DAOOLN010000000
VERSION     DAOOLN010001024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203661_length_1021_cov_3.292961"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PLW80_11735"
     CDS             complement(<1..866)
                     /locus_tag="PLW80_11735"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPB67230.1"
                     /translation="MRLAANFLDFTPPELLDGPGSVKRLPSFIKSKGFSRVLVVTDKG
                     LMAARLLDGLFDSLREAGVEYALFDGAQANPTIQNIEDALTLYKEKGCQAIIAFGGGS
                     SMDCAKGCAARVTNPGKTIPRMRGLLKVGRKPATVFAVPTTAGTGSETTIAAVITDPS
                     THEKYAINDPKLLPPYAVLDPELTVGLPPHITSTTGMDALTHAVEAYVGRSNTKNTIE
                     KAEKAVALIYANLETAYKDGKNVGARANMLLASYYAGIAFTRAYVGYVHAIAHNLGGL
                     YGLPHGLANALL"
BASE COUNT          168 a          316 c          350 g          187 t
ORIGIN      
        1 atcagtagcg cgttggccag accgtgcggc aggccgtaca gcccgccgag attgtgcgct
       61 atcgcgtgca cgtagccgac gtaggccctg gtgaacgcga tgcccgcgta gtacgaggcg
      121 agcagcatgt tggctcgcgc cccgacgttc ttgccgtcct tatacgccgt ctcgaggttc
      181 gcgtagatca gcgctacggc cttctcggcc ttctcgatcg tgttcttcgt gttgctacgg
      241 ccgacgtagg cctcgacggc gtgcgtcagc gcgtccatgc ccgtcgtcga ggtgatatgg
      301 ggcggcaggc cgacggtcag ctcggggtcg agcaccgcgt agggcggtag cagcttggga
      361 tcgttgatgg cgtacttctc gtgcgtcgaa ggatcggtta tgaccgccgc gatggtcgtc
      421 tcggaaccgg tgccggcggt cgtcggcacg gcgaagacgg tcgccggctt acgcccgacc
      481 ttgaggaggc cgcgcatcct cgggatcgtc ttgccggggt tggtaaccct ggcggcgcag
      541 cccttggcgc aatccatcga cgagccgccg ccgaaggcta taatcgcctg gcaacccttt
      601 tccttgtata acgtcagcgc gtcctcgata ttttggatgg tcgggttggc ctgggcgccg
      661 tcaaacagcg cgtactcgac gccggcctcg cggaggctgt cgaacagccc gtcgagcagg
      721 cgcgccgcca tgaggccctt gtcggtgacg acgagcaccc tcgagaagcc ctttgacttg
      781 atgaacgacg gaagacgctt gacgctgccg gggccgtcga gtagctcggg cggcgtaaag
      841 tcgaggaagt tcgccgcgag gcgcatgaca gcctggaaga gccggtacca cagtattttt
      901 ataagcatag tccccccgga acgcgggcca gccaaggcgg gccaggatca ggtcagggta
      961 gacccgccgc cccgggacgt caaggaagaa ttagccgggc ccggctattc gccgagcgcg
     1021 c
//
LOCUS       DAOOLN010001025         1021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_203701_length_1021_cov_2.749482, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001025 DAOOLN010000000
VERSION     DAOOLN010001025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203701_length_1021_cov_2.749482"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PLW80_11740"
     CDS             complement(<1..243)
                     /locus_tag="PLW80_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPB67231.1"
                     /translation="MSPSPRKARSGPGLLGFHHAAVICSDYERSKRFYVETLGLEPLA
                     EAWRPESCSWKLDLGAGAGYIVELFWFGERPPRPDRP"
     gene            complement(321..>1021)
                     /locus_tag="PLW80_11745"
     CDS             complement(321..>1021)
                     /locus_tag="PLW80_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPB67232.1"
                     /translation="TVPKEMLPLINKPSIAFVVDEFVASGIEDIVIITSRRKKSLDDY
                     FDHEAELEAVFSREGRDDKAALIAPPAARISFVRQTEMRGTGHALLQAAPLLGGEPCV
                     VAYPDDLHFGAPPLARQLMDVHERTGGCVLATVHDPGDVSRYGVVDPAPDGEGVRGFV
                     EKPTKGSEPSHEISIGRYLYTPEFFELLAEGWTRHEDGEYYHIYALDRMIAVGKVAYK
                     SVEGQRLDTGEPAG"
BASE COUNT          147 a          348 c          364 g          162 t
ORIGIN      
        1 gggacggtcg ggccggggcg ggcgctcgcc gaaccagaac agctcgacga tgtagccggc
       61 gcccgcgccc aggtcgagct tccaggagca cgactccgga cgccaggcct cggcgagggg
      121 ctcgaggccc agagtctcga cgtagaagcg cttggaccgc tcgtagtcgg agcagatgac
      181 cgcggcatgg tggaacccga gcaggccggg gccgctacgg gccttgcgcg gcgacgggct
      241 catttccgat tcgcgacgaa tcttcgtatc acctcgcgcc acgcgggctc gcgccaggcg
      301 tactcgagca cggcctccag ttagcccgcg ggctcgcccg tgtccaggcg ctggccctcg
      361 acgctcttgt aggccacctt gccgaccgcg atcatccggt cgagggcgta gatgtggtag
      421 tactcgccgt cctcgtgccg cgtccatcct tccgcgagca gctcgaagaa ctcgggcgtg
      481 tagaggtagc ggcctatcga tatctcgtgg ctcggctccg atcccttcgt cggcttctcg
      541 acgaagccgc gcacgccctc gccgtcgggg gccgggtcca cgacgccgta gcgcgacacg
      601 tcccccgggt cgtggaccgt ggccagcacg cagccgccgg tacgttcgtg cacgtccatc
      661 agctggcgcg cgagcggcgg cgcgccgaag tgcaggtcgt ccgggtaggc cacgacgcag
      721 ggctcgccgc cgaggagcgg cgccgcctgc agcagggcgt ggccggtgcc tcgcatctcg
      781 gtctggcgca cgaacgagat acgggccgcg ggcggcgcta tcagcgcggc cttgtcgtcg
      841 cgcccctcgc gggagaagac ggcctcgagc tcggcctcgt ggtcgaagta gtcgtcgagc
      901 gacttcttgc ggcggctcgt gatgatgacg atgtcctcga tgcccgacgc gacgaactcg
      961 tcgacgacga acgctatcga gggcttgttg atcaggggga gcatttcctt gggcacggtc
     1021 t
//
LOCUS       DAOOLN010001026         1020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_203967_length_1020_cov_3.477720, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001026 DAOOLN010000000
VERSION     DAOOLN010001026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203967_length_1020_cov_3.477720"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..>1020
                     /locus_tag="PLW80_11750"
     CDS             152..>1020
                     /locus_tag="PLW80_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB67233.1"
                     /translation="MDKLLEVKNLKLYYHTSKGAVRALDDVSFDLYKGETLGLVGESG
                     CGKTTTGVALLKMPSPPGRIEPGSQIILDGRDIMPLPEKEIRKNVRWEEVAMVFQGAM
                     NCLTPVYTIGKQMLETLQEHRDMDKAQAEELIKEYLGLVGLPPEVMGRYPHELSGGMK
                     QRIVIATALFLKPKLVILDEPTTALDVIVQAQIINLLKKLKKTFDLSFIFITHDLSLE
                     AEVSDRICVMYAGKIAELGTNQQIYGEQGPMHPYTEKLLQATPLLHKKVDSLSYIPGT
                     PPDLIDPPKGCRF"
BASE COUNT          220 a          339 c          292 g          169 t
ORIGIN      
        1 cgacgcgcag ctttccggag ccacgctgaa tgggctctgg tggtggatcg tcccgccggg
       61 actattcatc gccatcatgg gcatgacgtt cgccttcctg ggtttcgcca tggacaagat
      121 cctgcacccg aaactccgca cgaggtaaac catggacaaa ctactggaag tcaagaacct
      181 taagctgtac taccacacct cgaaaggggc ggtacgcgcc ctcgacgacg tcagcttcga
      241 cctgtacaag ggcgagaccc tcgggctcgt cggcgagtcg ggctgcggca agaccaccac
      301 cggcgtcgcc ctgctcaaga tgccgtcgcc ccccggccgc atcgagccgg gctcgcagat
      361 catcctcgac ggcagggaca tcatgcccct gcccgagaag gagatccgca agaacgtacg
      421 atgggaagag gtggcgatgg tgttccaggg cgcgatgaac tgcctgaccc cggtctacac
      481 gatcggcaag cagatgctcg agaccctgca ggaacatcgc gacatggaca aggcccaggc
      541 cgaggagctg atcaaggagt atctcggcct cgtgggtctg cctcccgagg tgatgggccg
      601 ctacccgcac gaactgtccg gcggcatgaa gcagcgcatc gtcatcgcga cggcgctgtt
      661 cctgaagccc aagctggtca tactcgacga gccgaccacg gccctcgacg tgatcgtgca
      721 ggcccagatc atcaacctgc tcaaaaagct caagaagacc ttcgacctgt ccttcatctt
      781 tatcacgcac gacctgtccc tcgaggccga ggtctcggac cgcatctgcg tcatgtacgc
      841 cggcaagatc gccgagctgg gcaccaacca gcagatctac ggcgaacaag gtccgatgca
      901 cccatatacc gagaagctat tgcaggctac gcccctcctc cataagaagg tggacagcct
      961 gtcgtacatc ccgggaaccc cgcccgacct gatcgacccg ccgaagggtt gccgtttcca
//
LOCUS       DAOOLN010001027         1020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_204176_length_1020_cov_1.644560, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001027 DAOOLN010000000
VERSION     DAOOLN010001027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204176_length_1020_cov_1.644560"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="PLW80_11755"
     CDS             <1..664
                     /locus_tag="PLW80_11755"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB67234.1"
                     /translation="GYVTGFIDSSLMLCVPMARELGLRLVLPEYRLAPEHPFPAAIDD
                     ALKAYRRLLSEGYAPGEMALAGDSAGGGLCLAVLQAIRDSGGPVPAAALCLSPWADLT
                     HSGRSHVENAATDPLLHLEMLKQWASYYAGGASLADPLVSPVFADFSGFPPLLLQVDS
                     GELLLDDSLAIAERARAAGVDARLIRREGLWHVWPALGDLIPESRQAFGAMKAFLASL
                     GG"
     gene            complement(803..>1020)
                     /locus_tag="PLW80_11760"
     CDS             complement(803..>1020)
                     /locus_tag="PLW80_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67235.1"
                     /translation="WNTNAGAQAFTALIAEKPAVIVIHNPDVQTYAKLIKKAEEEGIY
                     VIQINMRSAQASTAKAIRSTGPSDLPT"
BASE COUNT          129 a          341 c          347 g          203 t
ORIGIN      
        1 cggctacgtg accggcttca tcgactcgtc gctaatgctc tgcgtgccga tggcgcggga
       61 gctcggccta cgcctggtgc ttccagagta ccggctcgcg cccgagcatc ccttccccgc
      121 ggcgatcgac gacgcgctca aggcctaccg ccggcttctg tccgaaggct acgcgcccgg
      181 cgagatggcg ctggccgggg actcggcggg aggcgggctg tgcctggccg tgctccaggc
      241 catccgcgac tcgggcgggc ccgtgcccgc cgcggccctg tgcctgtcgc cctgggccga
      301 cctgacgcac tccggccgta gccacgtcga gaacgccgcc acggatcccc tcctgcacct
      361 cgagatgctc aagcaatggg cgtcgtatta cgccggcggg gcttctctgg ccgatcccct
      421 ggtctctccg gtcttcgcag atttctccgg cttcccgcct ttgctcctgc aggtagattc
      481 cggggagctg ctgctcgacg actcgttggc gatagccgag cgggcgaggg cggccggcgt
      541 ggacgcgagg ctgatacgcc gcgaggggct gtggcatgtc tggccggcgc tcggcgacct
      601 gatacccgag agccggcagg ctttcggtgc tatgaaggcg tttcttgcgt cgttgggcgg
      661 gtgacgatcg accccagcct tgtccgcgct cggccgtccg gggtatactc gtcggtatga
      721 atatagcctc attcgacgac tgggcccggg agctcctggg tatagagcgt ttcaggtcca
      781 tcgacgactc gctcaacggg cttcaggtcg gcaggtccga cgggccggtc gagcggatag
      841 ccttcgcggt cgacgcctgc gccgagcgca tgttgatctg gatcacgtag atgccttcct
      901 cctccgcttt cttgatcagc ttggcgtagg tctgcacgtc ggggttgtgg atcacgatca
      961 ccgccggctt ctcggcgatc agtgccgtga acgcctgggc gccggcattg gtgttccagt
//
LOCUS       DAOOLN010001028         1019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_204310_length_1019_cov_2.884855, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001028 DAOOLN010000000
VERSION     DAOOLN010001028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204310_length_1019_cov_2.884855"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1019
                     /locus_tag="PLW80_11765"
     CDS             <1..>1019
                     /locus_tag="PLW80_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HPB67236.1"
                     /translation="DAFYELCDELGLLVWQDCMFSCALYPSSKEFLSSVEAETRHQVR
                     RLASHPSLALWCGNNEALGAITWYDESRNSPARYIVDYDRLTEGTVGRVIHELDPGRT
                     WWPSSPSAGPDDFADNWHSDSRGDMHYWSVWHEGRPFSDYLSVRPRFCSEFGFQSFPS
                     HHTVASFAPDGERNVSSPAMEHHQRHPRGNALIVETMLRYFRMPRGLRETLYLSQVQQ
                     AMAIKTAVEYWRCNRPRCMGALYWQLNDVWPGASWSSLEYDGSWKLLHHEAKRFFDPL
                     IAAAFVRDGSVQVYAVNDGAEALEAEARLTLRRFDGSAAAELATFDSVLGAEAATPLW
                     SAPVA"
BASE COUNT          158 a          362 c          329 g          170 t
ORIGIN      
        1 cggacgcgtt ctacgaactg tgcgacgagc tcggcctcct cgtctggcag gactgcatgt
       61 tctcctgcgc gctctatccg tcctcgaagg agttcctgtc ctcggtcgag gcggagaccc
      121 gccaccaggt caggcgcctc gccagccacc cgtcgctggc gctctggtgc ggcaacaacg
      181 aggcgctcgg cgcgatcacc tggtacgacg agtcgcggaa cagccccgcc aggtatatcg
      241 tcgactatga ccgcctgacc gagggcactg tcggccgcgt catccacgaa ctcgacccgg
      301 ggcggacctg gtggccgtcg tcgccgtcgg ccggcccgga cgacttcgcc gacaactggc
      361 actccgactc ccgcggcgac atgcactact ggtccgtctg gcacgaggga cgccccttct
      421 cggactacct gagcgtgcgg ccgcgtttct gctcggagtt cggctttcag tcctttccat
      481 cgcaccacac ggtcgcctcc ttcgcgccgg acggcgagcg caacgtctcg tcgccggcga
      541 tggaacacca ccaacgtcac ccccgcggta acgcgctgat cgtcgagacg atgctacgct
      601 acttcaggat gccacgcggc ctcagggaga cgctgtacct gtcccaggtg cagcaggcga
      661 tggccatcaa gacggccgtg gagtactggc gctgtaatcg gccgcgctgt atgggcgcgc
      721 tatactggca gctcaacgac gtatggccgg gcgccagctg gtcgagcctc gagtacgacg
      781 gctcatggaa gctcctgcac cacgaggcca agcgcttctt cgacccgctc atcgcggcgg
      841 ccttcgtacg cgacggttcg gtacaggtgt acgccgtcaa cgacggcgcg gaggcgctcg
      901 aggccgaggc gaggctgaca ctgcgccgct tcgacggctc ggcggccgcg gagttggcaa
      961 cattcgactc ggtcctgggc gccgaggctg ccacgccgct atggagcgcc ccggtcgcc
//
LOCUS       DAOOLN010001029         1017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_205108_length_1017_cov_1.841996, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001029 DAOOLN010000000
VERSION     DAOOLN010001029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205108_length_1017_cov_1.841996"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="PLW80_11770"
     CDS             <1..986
                     /locus_tag="PLW80_11770"
                     /inference="COORDINATES: protein motif:HMM:NF014718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIF1 family ATP-dependent DNA helicase"
                     /protein_id="HPB67237.1"
                     /translation="MVRADLLDEMDRRMRQAKREPGLAFGGDRVILFGDPYQLRPVEP
                     DEASRSGGAFAERYKSPFFFSSRVLRARARTMKRVEFTRVFRQCEDGFIATLDRCRSG
                     DVSDDDLRLLASRVLPEGKKPPADHLVLTATRAEAQRINVYRLAALPGPSRVYAASAD
                     GRFAKDSGEDELPVPRELELKLGARVMLTASDEAGRWSNGALATVVGLDEAGATLRDD
                     AGEFRVGPHVWKRTRFALRDGGVVPVESDRYGQLPLQPGWALTIHRAQGLTLERVFVD
                     LSRGAFAAGQAYVAISRVTRLDGLLLRARPRAKDFFVHERVSAFLEYLKRQ"
BASE COUNT          151 a          335 c          357 g          174 t
ORIGIN      
        1 cgatggtccg cgccgacctc ctcgatgaga tggatcgacg gatgcgccag gcgaaacggg
       61 agcccgggct ggcgttcggc ggagaccgcg tcatcctctt cggcgatcca taccaactac
      121 gtccggtcga accggacgaa gcgagtaggt cgggcggcgc gttcgccgaa cgctataaaa
      181 gccctttctt cttctcctcg agagttctgc gcgctcgagc gcggacgatg aagcgcgtcg
      241 agttcacccg ggtcttcagg cagtgcgagg atggcttcat agcgacgctg gaccgttgcc
      301 gctcgggcga cgtgagcgac gatgacctgc ggctcctggc ctccagggtc cttcccgagg
      361 gtaagaagcc cccggccgac catctcgtgc tgacggccac gagggccgag gcccagcgta
      421 tcaacgtgta ccggctcgcg gccttgccgg gcccgtcccg cgtctacgcc gcgagcgctg
      481 acggccgctt cgccaaggac tcgggcgagg acgagctgcc ggtgccgcgc gagctcgagc
      541 tcaagctcgg cgctagggtc atgctcacgg cgagtgatga ggccggtcga tggtcgaacg
      601 gcgccttggc taccgtcgtc gggctcgatg aggctggcgc cacgttgcgc gacgacgccg
      661 gtgagttcag ggtcgggccc cacgtctgga aacggactcg gttcgccctc cgtgacggcg
      721 gagtcgttcc tgtcgagtcg gatcggtacg ggcagctgcc gctccagccg ggctgggcct
      781 tgacgataca tagggcccag gggctcacgc tcgagcgcgt cttcgtcgac ctgtcccggg
      841 gcgccttcgc cgccgggcag gcctacgtgg ccatctcccg cgtaaccagg ctcgacggcc
      901 tcctgctccg ggcgcggccc cgggccaagg acttctttgt ccatgagagg gtctcggcct
      961 tcttggagta cctcaagcgg cagtaacccg ggcgcggtat ggcgatgata tgggagg
//
LOCUS       DAOOLN010001030         1016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_205292_length_1016_cov_2.841831, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001030 DAOOLN010000000
VERSION     DAOOLN010001030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205292_length_1016_cov_2.841831"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..>1016
                     /locus_tag="PLW80_11775"
     CDS             60..>1016
                     /locus_tag="PLW80_11775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HPB67238.1"
                     /translation="MARTDDEAASVGLVDRYRDRLRATHYSPMTERAYLYWLERYLKG
                     RPLPKPSDRPEASINAFVTKLAVQENVSASTQNQALAAILFLYRQVLAVEVGDLGELI
                     RAKRPIRMPVVLSPEEVRSVLTVMSGEMRLMASLLYGTGMRLNECLSLRVQDIDFNRN
                     TVIIRDGKGGKDRGTILPSSLIKPLQDHLKRVRAIHEADLKDGWGKAQLPTSLEKKYP
                     NAASEWIWQWVFPQKRRWRDSKSKSEGRFHMDASILQRAVREAMIIAGITKHASCHTF
                     RHSFATQLLENGYDIRTVQELLGHSDIRTTMVYTHVLNKGPGG"
BASE COUNT          251 a          267 c          287 g          211 t
ORIGIN      
        1 cgagttcatc gagtcgaata gcgccggaaa aagggggccg catcgtagcc cccgggccga
       61 ttgcgcgtac cgatgatgaa gcggctagcg tcggcttagt cgatcggtac cgcgatcggt
      121 tgcgggcaac tcattatagc ccgatgactg agcgagccta tctctactgg ctcgagcgtt
      181 acttgaaggg gcgtccccta ccaaagccta gtgatcgtcc cgaggcgtca ataaacgcct
      241 tcgtgacgaa gctagcggta caagagaacg tcagtgcctc gactcagaac caagccctag
      301 ccgctatact gttcctgtac cgacaggtac tcgctgtcga ggtgggagac ttgggagaac
      361 tgatacgagc aaaaaggccg atcaggatgc cggttgtctt aagccctgaa gaggtgcgat
      421 cggttcttac agtaatgagc ggcgagatgc ggctcatggc ctccttgctc tacggaacgg
      481 gcatgcgctt aaacgaatgt ctttcgctca gggtacagga tatcgatttt aataggaaca
      541 ccgttatcat ccgcgatggc aaaggaggca aggatagagg cactattctg ccttcgtccc
      601 tcatcaaacc gcttcaggat catcttaagc gcgtcagagc tattcacgag gccgacttga
      661 aagacggttg gggcaaggcc cagttaccga cgtcactcga gaagaagtat cctaacgcgg
      721 cttccgaatg gatatggcag tgggtattcc cgcagaaacg acgatggcgc gactcgaaat
      781 ctaagtcgga agggcgattc catatggacg cctccattct gcaacgagct gtacgtgaag
      841 ctatgataat cgcaggcatc acgaaacatg ccagttgcca tacattcagg cattctttcg
      901 ccacgcagct acttgagaac ggctatgaca tcaggacggt tcaggagctc cttggccata
      961 gcgacatccg tacaacgatg gtctacacgc atgttctcaa caaagggccg ggcggg
//
LOCUS       DAOOLN010001031         1015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_205645_length_1015_cov_2.877083, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001031 DAOOLN010000000
VERSION     DAOOLN010001031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205645_length_1015_cov_2.877083"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..768)
                     /locus_tag="PLW80_11780"
     CDS             complement(58..768)
                     /locus_tag="PLW80_11780"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HPB67239.1"
                     /translation="MRIRTAAALACLACAVAARDAFAFDVTSVPLHLYLSGLKEARPP
                     VVVDDYLVLSAQGAYRYVGAAFSYEGWRSVHSFEVNRYGVFVLAVPIPYGDAVTVRYR
                     LVLDGLWAADPANPAMERDGETGAAMSLATLPERPRTVLGVWDPAGDGAATFHFEGEP
                     GQRITVAGSFNGWDPFIHELEERSPGRYELRLSLPPGEHYYVFVYRGERIADPLNRRL
                     LYGKDGRPVSAITIAAAD"
BASE COUNT          190 a          333 c          329 g          163 t
ORIGIN      
        1 gcgtatccta gaggcgctct agtcgcttcc ggcttatttc cgcccagcgc ccggacgtca
       61 gtccgcggcc gcgatcgtga tggctgagac gggtcggccg tccttgccgt agagcaggcg
      121 ccgattgagc ggatcggcga tgcgttcccc gcgatagacg aagacatagt agtgctcgcc
      181 cggcggcagg cttagcctta gctcgtagcg ccccggcgat cgctcctcca gctcgtggat
      241 gaacggatcc cagccgttga acgacccggc gaccgtgatg cgctggccgg gctcgccctc
      301 gaaatggaag gtcgccgcgc cgtccccggc cgggtcccag acgccgagga ccgtcctcgg
      361 ccgttcgggg agcgtggcca gcgacatggc cgcgcccgtc tcgccgtcgc gctccatcgc
      421 cggattggcc ggatcggcgg cccagagccc gtcgaggacc agccgatagc gcacggtaac
      481 cgcgtcgcca taggggatgg gcaccgccag cacgaagacg ccgtagcgat tcacctcgaa
      541 cgaatggacc gagcgccagc cctcgtacga gaaggccgcc ccgacatagc ggtacgcgcc
      601 ctgggcgctc agcacgagat agtcgtccac caccacagga ggcctcgcct ctttgagccc
      661 cgacaggtag aggtgcagcg gcacggacgt cacgtcgaag gcgaaagcgt ctctagcggc
      721 gactgcgcag gcgaggcagg cgagcgccgc ggctgtacga attctcatcg atgctccaat
      781 agggtgatgc tcctatagta tcggcccgcc cagccgtccg cttgaggtcg gtctgagggc
      841 cgaaggcggc cacagcgttt tcgtcctata catccttaat tttttgccgt tattataagc
      901 atcagccatg ccaacactac aggatataga gcgataaaaa gcctcactca agacgctcgg
      961 caaggaaccg gagacgctgg agcgatgggg cgagacctgg catgacctgg aggcg
//
LOCUS       DAOOLN010001032         1015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_205792_length_1015_cov_2.007292, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001032 DAOOLN010000000
VERSION     DAOOLN010001032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205792_length_1015_cov_2.007292"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PLW80_11785"
     CDS             <1..322
                     /locus_tag="PLW80_11785"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPB67240.1"
                     /translation="TMRALGMSSGAVSRLFVVEALGLALMSSAGGLVAALVLFKLAGF
                     IDLSSVPSAGLFTERGRLRFYIDARIMTLNLAIMVGGVVLAALGPARKASLIHPADAM
                     RDEA"
     gene            379..>1015
                     /locus_tag="PLW80_11790"
     CDS             379..>1015
                     /locus_tag="PLW80_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HPB67241.1"
                     /translation="MNRYRLPIALSIALAALGSVQAQGTDYSELLKRVDATVTFEGSD
                     LSAEYTIVKRDPGGATSTTVATMFRRDATGQFLILILAPAIDKGKGYLKEGDNLWLYD
                     PVDRVFTFTSAKERFQNSSFRNSDFNRSNFSKDYTPVSGTRKKLGKFECTVLELRATN
                     DTVSFPRMRIWVTDDYLVRKIEDYSLSGQLMRTSAIPSYQKVGDKWVAAGMV"
BASE COUNT          202 a          295 c          305 g          213 t
ORIGIN      
        1 tacgatgcgc gccctgggca tgagttcggg cgccgtgtct cgtctcttcg tcgtcgaggc
       61 gctcgggcta gccctgatgt cgtcggccgg aggcctggtc gcggcactcg tcctgttcaa
      121 gctagccgga ttcatagacc tgtcgagcgt accgtcggct ggcctcttta ccgagagggg
      181 gaggctacgc ttctatatcg acgcgcgcat catgaccttg aacctggcca tcatggtcgg
      241 cggcgtcgtg ctggcggccc tgggacccgc gcgcaaagcc tccttgatcc acccggcgga
      301 tgcgatgcgc gacgaggctt gaaaccggaa ggcccgggcg tttgttacga tagtacattc
      361 cgttagtaaa ggatgctcat gaacagatat agactgccta tagccttgag tatagccttg
      421 gcggcccttg gctccgtaca ggcgcagggt acggactata gcgagctcct caagcgagtg
      481 gacgccaccg ttaccttcga gggatctgac ttgtccgccg agtacaccat cgtcaaacgc
      541 gatcccggcg gggcgacgag tacgacggtg gccacgatgt tccgccgcga tgccaccggc
      601 caattcctga ttctgatcct ggcgccggcc atcgacaagg gcaagggcta cttgaaggaa
      661 ggcgacaacc tttggctcta cgaccccgtg gaccgggtct ttacctttac gagcgccaag
      721 gagcgtttcc agaattcaag cttcaggaat tctgatttca atcggtctaa tttctccaag
      781 gactatacgc ccgtttccgg gacgaggaaa aagttaggca agttcgaatg cacggtgctc
      841 gagctccggg ccaccaacga tacagtatcc tttcctcgca tgcgtatctg ggtaaccgac
      901 gattacctgg tccgtaagat cgaggactat agcttgtccg gtcagctgat gcgcacaagc
      961 gccataccga gctaccagaa agtgggcgac aagtgggtcg cggcgggcat ggtca
//
LOCUS       DAOOLN010001033         1014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_205983_length_1014_cov_2.938478, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001033 DAOOLN010000000
VERSION     DAOOLN010001033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205983_length_1014_cov_2.938478"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PLW80_11795"
     CDS             complement(<1..533)
                     /locus_tag="PLW80_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPB67242.1"
                     /translation="MARVIVDGIGLEYSILAAASGAADGERSEPPTVALLNGIGMSIA
                     HWRPVAERLAAAGYRVLLHDFRGQLLSERPAGPYSFEGHARDLAGLLDALGLGRVVAV
                     GTSYGSEVALTFARDYPGRCSGVAAIDGVSEYDEVLRAAIECWRSAALADPRTFYKSL
                     VPWTYSAEYIAAYRSQLA"
     gene            complement(536..>1014)
                     /locus_tag="PLW80_11800"
     CDS             complement(536..>1014)
                     /locus_tag="PLW80_11800"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB67243.1"
                     /translation="GIALVPQERELFPGMSVADNLELGAAYVPRGRDIIEKQLDTVLS
                     LFPILKERYRQLVGTMSGGQQRMVAIGRALMANPILLILDEPSLGLQPSIVAEVFDRL
                     SVLKKDVAILVTEQNVRESLRAIDRGYVIENGRVLLEDTAEGLAKNPHVVSAYLGL"
BASE COUNT          160 a          350 c          353 g          151 t
ORIGIN      
        1 gcgagctggc tgcgatacgc cgctatgtac tcggccgagt aggtccaggg cacgagggac
       61 ttgtagaagg tccgggggtc ggcgagcgcc gccgatctcc agcattcgat ggcggcgcgg
      121 agcacctcgt cgtactcgct cacgccgtcg atcgcggcga cgcccgagca acggcccggg
      181 tagtcccgcg cgaaggtcaa cgcgacctcc gagccgtagg aggtgcctac ggcgacgacg
      241 cggccgaggc cgagtgcgtc gagcagcccg gcgaggtcgc gggcgtgccc ctcgaacgag
      301 tacggccccg cgggccgctc cgacaggagc tgaccgcgga agtcgtggag caggacgcgg
      361 tacccggcgg cagctagccg ctcggctacc ggccgccagt gggcgatcga catgccgatg
      421 ccgttgagca gcgcgaccgt cggcggctcg ctccgctccc cgtcggcggc cccgctcgcc
      481 gcggccagga tcgagtactc gaggccgatc ccgtcgacga tcacgcgcgc cacggctaca
      541 gccccaggta ggcggacacg acgtgggggt tcttggccag gccctcggcc gtgtcctcga
      601 gcagcacccg cccgttctcg atcacgtagc cgcggtctat ggcacgcagg ctctcgcgta
      661 cgttctgctc ggtgacgagt atggccacgt ccttcttgag gacagagagc cggtcgaaca
      721 cctccgccac gatcgacggc tggagtccga gcgacggctc gtcgagaatc aagaggatcg
      781 ggttggccat cagcgcgcgg cctatcgcca ccatgcgttg ctggccaccg gacatcgtgc
      841 cgacgagctg cctgtagcgc tccttgagta tcgggaagag gctcagcacc gtgtcgagct
      901 gtttctcgat gatatccctg ccgcgcggca cgtaggccgc cccgagctcc aggttatcag
      961 cgacgctcat gccgggaaac agctcgcgct cctgcggcac gagggctatg ccgg
//
LOCUS       DAOOLN010001034         1014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_206087_length_1014_cov_2.201251, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001034 DAOOLN010000000
VERSION     DAOOLN010001034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_206087_length_1014_cov_2.201251"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PLW80_11805"
     CDS             complement(<1..273)
                     /locus_tag="PLW80_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67244.1"
                     /translation="MDKLKQKLAAFMSGRYGMDDLNKGLVGLYIVILLVNMGLRSQIL
                     SALIWPIMIYAIYRSYSRNVAARSAENDKYLALTKPRVVYLIVFTAL"
     gene            complement(286..>1014)
                     /locus_tag="PLW80_11810"
     CDS             complement(286..>1014)
                     /locus_tag="PLW80_11810"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPB67245.1"
                     /translation="GRSAQEGEGRTMTKDPYQVLGVSPGASDDDIAKAYRKLAKTYHP
                     DVNHGSPEAAAKMSEINAAYERIKSGAAAQGSGPYQGQAGYGAQGSYGQAGYGQGGQG
                     SSDPFGFGFDPFEFFSGGFSGGRQGASPLGQARSYINAGLFAKADDLLKSMDDRGAEW
                     HCLRAVAQSGMGNQIDALRHAKTAVQMEPDNPEYRGVLNQLQNGGQAYRQRSQGYGMP
                     TVNLGNICLGVILTNLFCTFCRPF"
BASE COUNT          151 a          293 c          362 g          208 t
ORIGIN      
        1 gagggccgtg aagacgatca ggtagacgac gcgcggcttg gtcagggcca ggtacttgtc
       61 gttctcggcc gagcgcgcgg ctacgttccg cgagtagctg cgatagatgg cgtagatcat
      121 gatcggccag atcagggccg acaggatctg ggatcggagc cccatattga cgagcaggat
      181 gacgatatac aggccgacga ggcctttgtt caggtcgtcc atgccgtatc ggccgctcat
      241 gaaggcggct agtttctgtt tcagcttatc catggatgcg ttcctttaga atggcctgca
      301 gaaggtgcag aacaggttgg tgaggatgac gccgaggcag atgttgccta ggttgacggt
      361 gggcatgccg tagccctggc tgcgctgacg gtaggcctgg ccgccgttct ggagctggtt
      421 cagcacgcct cggtattcgg gattgtccgg ctccatctgc acggccgtct tggcgtgcct
      481 gagggcgtcg atctggttac ccatgccgga ctgggccacg gcgcgtaggc agtgccactc
      541 ggcgccgcgg tcgtccatcg acttgaggag gtcgtcggcc ttggcgaata gtcccgcgtt
      601 tatatacgag cgcgcctggc cgagcgggga ggccccctgg cggccgcctg agaagccgcc
      661 tgagaagaat tcgaaggggt cgaagccgaa gccgaacggg tctgacgagc cctggccgcc
      721 ctggccgtag ccggcctgtc cgtaggagcc ctgggcgccg tagccggcct ggccctggta
      781 cggccccgaa ccttgggcgg cggcgccgct cttgatgcgt tcgtaggcgg cgttgatctc
      841 gctcatcttg gcggcggcct cggggctgcc gtggttgacg tcggggtggt aggtcttggc
      901 cagcttgcgg taggccttgg ctatatcgtc gtccgaggcg cccggcgata cgccgagcac
      961 ctggtatgga tccttggtca tcgtgcggcc ttctccttct tgagcactgc ggcc
//
LOCUS       DAOOLN010001035         1012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_206692_length_1012_cov_2.660397, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001035 DAOOLN010000000
VERSION     DAOOLN010001035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_206692_length_1012_cov_2.660397"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..238)
                     /locus_tag="PLW80_11815"
     CDS             complement(77..238)
                     /locus_tag="PLW80_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67246.1"
                     /translation="MAGTTDRMDDSIRILISSIDKGRVKKMYEERGENLSEDVRAYLF
                     RKLARHEKV"
     gene            complement(529..604)
                     /locus_tag="PLW80_11820"
     tRNA            complement(529..604)
                     /locus_tag="PLW80_11820"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            complement(659..732)
                     /locus_tag="PLW80_11825"
     tRNA            complement(659..732)
                     /locus_tag="PLW80_11825"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(697..699),aa:Thr,seq:cgt)
BASE COUNT          174 a          339 c          273 g          226 t
ORIGIN      
        1 cagcaccttg tacgacttag ggttgccaaa gggatcgaac gtgatgccgt caacctcggt
       61 tcgccgggcg ttgtacttag accttctcat gccgcgccag cttgcggaaa aggtacgccc
      121 tcacgtcctc gctgagattc tcgccgcgct cctcgtacat cttcttcacc cgccccttgt
      181 cgatgctcga tatcaatatc cttatcgagt cgtccatcct gtcggttgtg cctgccatgc
      241 ttcctcctgg tatcccctac tatcgctcgg ggagtcgcgg agttcttccc gctcaagggc
      301 gacgcttcat tccttcggtg gcgaaccctt agctcgaaac gcacgagccg cgtccggtgt
      361 actgcccgcc ggtagacagc caggtggtca agcgcgggcg cttcccggtt gcctggactt
      421 agaaaggatg ccccgtatcc gccggggcca cgcggccgcg ttactgttta cggacagcgg
      481 gcgtatccat ccaccgttcc ggtaaaaacc ggcgccgtgg tcgcgcgctg gaagcggcgg
      541 gagtcgaacc cgctatttca gtaccgcacg gcgtcccgcg ctgtctggct ccgactacgc
      601 tccctggtgc cctgcgttga tagccaacgg ccggcgcagg gtcagaagac tcagaactag
      661 cacccggccc ggaatcgaac cggcaaccta cggtttacga aaccgttgct ctaccaactg
      721 agctacgggt gcaggtgccg tccatctcca acggagccag gacggcgtag tatatccatg
      781 aagaatcagt actatccttt cgtatctttg attccagccc atgcgggccg gtattgctat
      841 cataccacgt ctagcgtggc attgcaatcc ctagtttatc gcgtctttcc ggcgcaagta
      901 gacctcgctc gacttctcga cctcgccgag ctctccggcc ttcgcgcatt ccggccctag
      961 cttccccttg accttgtact ggcaccgatt cggcgatacg cagtgcccgg cg
//
LOCUS       DAOOLN010001036         1011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_207082_length_1011_cov_2.281381, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001036 DAOOLN010000000
VERSION     DAOOLN010001036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207082_length_1011_cov_2.281381"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1011)
                     /locus_tag="PLW80_11830"
     CDS             complement(<1..>1011)
                     /locus_tag="PLW80_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPB67247.1"
                     /translation="LATGRTVEIGEAVGIIAAQSIGQPGTQLTMRTFHIGGAATKASE
                     ENRIALKYPVYVTEIVGLYVELPNGHSLFTRKGYLMSCKVFGQWELEKGDKLLVEDGR
                     RILRGDELIKRKSGEVVKATDISYAKVVETAKGAPVKLLSIAQEARVEVRNGSEIVVS
                     VGEVVPAGNALAIFDPFSDPIISEYDGYVRYEDIIPGSTLKEEINEDTGNVEKKIAEF
                     GTERDAKQPRIVIADEAGNEIFTYLLPGGAYLNVEDGELVKAGKTLARTLKEGARAMD
                     IVGGLPRVGELFEARKPKTQTVLAMVSGKVGFRGIVKGKRVINILDLFGKEYKHLVPV
                     GR"
BASE COUNT          146 a          331 c          331 g          203 t
ORIGIN      
        1 ctgcggccga ccggcaccag gtgcttgtac tccttgccga acaggtcgag gatgttgatg
       61 acgcgcttgc ccttgacgat gccgcggaag ccgaccttgc cggacaccat cgcgagcacc
      121 gtctgcgtct tgggcttgcg ggcctcgaac agctcgccta cccgcgggag gccgcctacg
      181 atatccatcg ccctggcgcc ttccttgagc gtcctcgcaa gtgtcttacc ggccttgacg
      241 agctcgccgt cctcaacgtt gaggtaggcg ccgcccggca gcaggtaggt gaagatctcg
      301 ttgccggcct cgtcggcgat gacgatgcgc ggctgcttgg cgtcgcgctc ggtgccgaac
      361 tcggctatct tcttctcgac gttgcccgta tcctcgttga tctcttcctt tagggtcgag
      421 ccggggatga tatcctcgta gcggacgtag ccgtcgtact cggagatgat cgggtcggag
      481 aacgggtcga agatggccag cgcgttgccg gccggcacga cctcgcccac ggacacgacg
      541 atctcggagc cgttcctgac ctctacgcgg gcttcctggg ctatcgacag cagcttgacc
      601 ggggcgccct tggccgtctc gacgaccttg gcgtaggaga tgtcggtcgc cttgacgacc
      661 tcgccggact tgcgcttgat cagctcgtcg ccgcgcagga tgcgcctgcc gtcctcgacg
      721 agcagcttgt cgcccttctc gagctcccac tggccgaaga ccttgcagga catcaggtag
      781 cccttgcggg tgaacaggga gtggccgttg ggtagctcga cgtacaggcc gacgatctcg
      841 gtcacgtata ccgggtactt gagcgcgatg cggttttcct cggaggcctt cgtcgccgcg
      901 ccgccgatgt ggaaggtacg catcgtcagc tgggtacccg gctggccgat cgactgggcc
      961 gcgatgatgc cgacggcctc gccgatctcg acggtgcgcc cggtcgcgag g
//
LOCUS       DAOOLN010001037         1011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_207113_length_1011_cov_2.087866, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001037 DAOOLN010000000
VERSION     DAOOLN010001037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207113_length_1011_cov_2.087866"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..>1011
                     /locus_tag="PLW80_11835"
     CDS             193..>1011
                     /locus_tag="PLW80_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67248.1"
                     /translation="MEPTLSYRLFAARFAPARLFAVAAAAMAAFVACQAASEMEAAIR
                     LPTMPSAWAGFGGAPGAGLWELSWLSASGSGGPFPAAPGAVVALRLPRGEEAAVFCRA
                     ALGAARSLPYGAAWPQALDASGELAPTAAGGFAAEAAACLYRLGLRHCGLDLVRLAAE
                     AEARMADPWDADPSAIATAALGGRLRADHLREPERVGLTVTVLPGPARPDSPYGLVIE
                     PGPGGSATVSVPIGRVRRWIGDGYELVVAVSPSGEPAWTLSTSTASRLIEVDTAT"
BASE COUNT          122 a          361 c          367 g          161 t
ORIGIN      
        1 tgcttgagtt ccgagccatc catatgttcg atcttggcgg acagggcccg ctcgagcctg
       61 tccgcgtaca caacgctgtc ttccatcgtt gcgcacagta tggaataaaa ccgccgcgga
      121 ggcaagctcc catcgccagc tcgacgatat gcgggtcgca agcgcggagg cccgcgcccg
      181 cgtgtacgcg gcatggaacc aacgctttcg tacaggctat tcgcggctcg gtttgcgccg
      241 gcccgcctat tcgccgtcgc ggccgccgcc atggccgcgt tcgttgcttg ccaggcggcc
      301 tctgagatgg aggcggcgat acgcttgcct accatgccgt cggcctgggc cggtttcggc
      361 ggagcccctg gagccgggct ctgggagctg tcctggctgt cggcctcagg ctccggggga
      421 ccgttcccgg cggcgccagg cgcggtcgtc gcgcttcgcc tgcctcgcgg cgaggaggca
      481 gcggtcttct gcagggccgc ccttggcgca gcgcgtagcc tgccctacgg ggcggcctgg
      541 cctcaggccc tggacgcctc tggcgagctg gcgccgacgg cggccggggg cttcgcggcc
      601 gaggccgccg cctgcctcta taggctcggg ctgcggcatt gcgggctgga cctggtacgg
      661 ctggcggcag aggcggaggc caggatggcc gacccatggg acgctgaccc gtccgccata
      721 gcgaccgccg cgcttggggg gcgtctgcgg gccgaccacc tgcgcgagcc cgagcgggta
      781 gggctgaccg tgaccgtgct acccgggccg gcccggcctg acagccccta cgggctcgtc
      841 atcgagccgg ggccaggagg ctcggcgacc gtttcggtcc cgataggccg cgtccgccgc
      901 tggataggcg acggctacga gctcgtcgtc gcggtatcgc cgtccggcga gccggcctgg
      961 acgctgtcga cgtcgaccgc cagccgcctc atcgaggtcg acaccgccac c
//
LOCUS       DAOOLN010001038         1010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_207276_length_1010_cov_3.780105, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001038 DAOOLN010000000
VERSION     DAOOLN010001038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207276_length_1010_cov_3.780105"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PLW80_11840"
     CDS             <1..457
                     /locus_tag="PLW80_11840"
                     /inference="COORDINATES: protein motif:HMM:NF020071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HPB67249.1"
                     /translation="VSDALAAGAVDWLSDTGPVIDGASLVAMLAGRLGPDGVRGLDRA
                     APPFVETPGGRKRRPTYPVAGPARLAGRIQEFFGMEDGPRACGEPMTLELLSPADRPL
                     QVTSDLAGFWRNTYPSIRAEMARRYPRHYWPTDPLAAEPTRGQRPRPSA"
     gene            complement(447..>1010)
                     /locus_tag="PLW80_11845"
     CDS             complement(447..>1010)
                     /locus_tag="PLW80_11845"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HPB67250.1"
                     /translation="GAGIASGLVTVLAKNVFSRGMLELSEEPLSAMLVEVNRRMIREL
                     SAVGNTVSCAFMRFAGDRVEYVNAAHTDALLRRAGKRAVSFVKAKQGPSKAPPLGRDD
                     FEARVGALAFSAKPGDALLVYTAGLVNGANAKGAPYGVERLSEAFARADPDSAESTLA
                     SLMIDYRNYLAGSRRGGDVGVMVFVKR"
BASE COUNT          145 a          354 c          363 g          148 t
ORIGIN      
        1 cgtctccgac gccctggcgg ccggcgccgt ggactggcta tcggataccg gtccggtcat
       61 cgacggcgcg tccctcgtcg ccatgctcgc cgggaggctc ggcccggacg gcgtccgagg
      121 gctcgaccga gccgcgccgc ccttcgtcga gacgcccggc ggcaggaagc ggcgcccgac
      181 ctacccggtt gccggtccgg ccaggctggc cggccgtatc caggagttct tcgggatgga
      241 ggatgggccg agggcctgcg gggagcctat gaccctcgag ctcctgtcgc cggcggacag
      301 gccgctccag gtgacgagcg acctggccgg cttctggagg aacacctatc cgtcgatacg
      361 cgccgagatg gccaggcgct atcccaggca ctactggccg accgatccgc tcgccgccga
      421 gccgaccagg ggccagcggc ctcggcctag cgcttgacga agaccatcac gccgacgtcg
      481 ccgccgcgcc tcgagccggc caggtagttc cggtagtcta tcatcaagga ggcgagcgtc
      541 gactcggcgc tgtcggggtc ggccctggcg aaggcctcgc tgaggcgctc gacgccgtag
      601 ggcgctccct tggcattggc gccgttgacg agcccggccg tatagacgag gagcgcgtcg
      661 cctggcttgg cgctgaaggc gagagcgccg accctggcct cgaagtcgtc gcggccgagc
      721 ggcggagcct tggacggacc ctgcttggcc ttgacgaagg agaccgcccg tttgccggcc
      781 cgcctgagca gcgcgtcggt atgggccgcg ttgacgtact cgacgcggtc gccggcgaag
      841 cgcatgaagg cgcaggagac ggtgttaccg accgccgaca gctcgcggat catgcgccgg
      901 ttcacctcga cgagcatagc cgacagcggt tcttccgata gctcgagcat gcctcggctg
      961 aacacgttct tggcgagcac cgtgacgagg ccgctggcga tgcccgcgcc
//
LOCUS       DAOOLN010001039         1010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_207523_length_1010_cov_1.433508, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001039 DAOOLN010000000
VERSION     DAOOLN010001039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207523_length_1010_cov_1.433508"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PLW80_11850"
     CDS             complement(<1..720)
                     /locus_tag="PLW80_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67251.1"
                     /translation="MGLIQRSCIRSIHGLLASHRLVLVEGPPRSGRSRIAAQVAAGAP
                     GGGRLVDARKPEGRAVLADPASASAARLIVVDGAGRREAERVAAGLSAFGDVGRSPRF
                     ALIGGPFGRTAAGRTTEGREASALDAPIISIGPLSLFEAGSASMRRLWLRGGLPEAFM
                     SASDQAAFAWLRDYAACLAGGSLSAWGLPRDAAFVAGLLEGLAASSGRAFNENAAARS
                     LGVSRPTIGRAVAAVESAGLLF"
     gene            832..>1010
                     /locus_tag="PLW80_11855"
     CDS             832..>1010
                     /locus_tag="PLW80_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB67252.1"
                     /translation="MKTGSNRYDVCVIGAGVCGANIARRLSAYELDVALVDKEADVSF
                     GTSKANSGIVHGGFH"
BASE COUNT          165 a          355 c          350 g          140 t
ORIGIN      
        1 gaacaggagc cccgcgctct cgacggccgc gacggctcgt cctatcgtcg gcctggagac
       61 gcccaggctc ctggccgcgg cgttctcgtt gaaggcgcgc ccgctcgagg cggccaggcc
      121 ctcgagcagg ccggcgacga aggccgcgtc gcggggcagg ccccaggcgg acagcgatcc
      181 tccggccagg caggcggcgt agtcgcgcag ccaggcgaac gcggcctggt cgctcgccga
      241 catgaaggct tccggtaggc cgccgcgcag ccatagtcgc cgcatcgagg ccgagcccgc
      301 ctcgaacagg ctcaaggggc cgatgctgat tatcggcgcg tcgagcgccg aggcctcgcg
      361 gccctccgtc gtacggcccg cggccgtacg gccgaacggc ccgcctatca gggcgaagcg
      421 cggcgagcgc ccgacatcgc cgaaggcgct taagccggcg gcgacccgct cggcctcgcg
      481 ccggccggcg ccgtcgacca cgatcagcct cgccgccgac gctgacgccg gatcggcgag
      541 gacggcgcgc ccctccggct tgcgcgcgtc gacgagcctg ccgccgcccg gcgctccggc
      601 cgctacctga gccgctatcc tcgacctgcc tgagcgcggc ggcccctcga cgagtacgag
      661 ccgatggctg gcgagcagcc cgtggatgct tctgatgcag gagcgctgaa tcaagcccat
      721 gaaatgcaag tataacgttt aaaaagcggt tataaaagca gcgggagtat agcgatcatc
      781 ttgctagcgc ccgttttcgc cctatactga tagctagcaa gggggccagg tgtgaagact
      841 ggatcgaatc gctacgacgt ctgcgtgata ggcgccggcg tctgcggcgc caatatagcg
      901 cggcgcctgt cggcttacga gctcgacgtg gcgctcgtgg acaaggaagc ggacgtgtcg
      961 ttcggcacgt ccaaggccaa ctcgggcatc gtgcacggcg gcttccacca
//
LOCUS       DAOOLN010001040         1009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_207645_length_1009_cov_3.180294, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001040 DAOOLN010000000
VERSION     DAOOLN010001040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207645_length_1009_cov_3.180294"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PLW80_11860"
     CDS             complement(<1..609)
                     /locus_tag="PLW80_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67253.1"
                     /translation="MKRIVLAAAIAAAAIASASAWDLEAASGRFGVIIVNNQYDPLPL
                     VPVTNTLGGALAISYSKGFFLSLQPALDLYWTNYKWYEGRAVPTESETGEGNNAFVLG
                     FIVDLPLTASFRFGERLGGAFGLGPAFVLRASFKNDTTAGVEEAMAGNLAAIASYFWS
                     DGRWFYPSASLRFDVYLQQNFTFAFGVRGLMPVYNAWTGNERI"
     gene            complement(638..>1009)
                     /locus_tag="PLW80_11865"
     CDS             complement(638..>1009)
                     /locus_tag="PLW80_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67254.1"
                     /translation="EPMERAPEALRAAVAAIGGPVLLEARLPDGSRRAFVRGDPGQAA
                     PAWGHLYGDRACVATDDWRVVLADGASGVIVDPSPVPPYPGATVRDAVLLEDALILLW
                     EAGSFPYLWASGLVILDAPRK"
BASE COUNT          178 a          313 c          365 g          153 t
ORIGIN      
        1 gatgcgctcg ttgccggtcc aggcgttgta gacgggcatg agcccgcgga cgccgaaggc
       61 gaaggtaaaa ttctgctgta ggtagacgtc gaaacgcaac gaggcggagg ggtagaacca
      121 gcggccgtcg gaccagaagt acgaggctat agccgcgagg ttgccggcca tcgcttcctc
      181 gacgcccgcc gtcgtgtcgt tcttgaagga ggcgcgcagc acaaaggctg gcccgaggcc
      241 gaaggcgccg cctaggcgtt cgccgaagcg gaagctggcg gtcagcggca gatcgacgat
      301 gaagccgagc acgaaggcgt tattgccctc gccggtctcc gactcggttg gtacggcgcg
      361 gccctcgtac cacttgtaat tggtccagta gagatcgagc gccggctgga ggctcagaaa
      421 gaagcccttg gagtacgata tggccagcgc accgcccaag gtgttcgtga ccgggacgag
      481 cggcagcggg tcgtactggt tgttgacgat gatgacgccg aagcggcccg acgccgcctc
      541 caggtcccat gcgctcgcgg aggcgatcgc ggccgctgca atggccgccg ccaagacgat
      601 tctcttcatc gtattgctac tatagcgcgc cgacggctca tttcctaggg gcgtcgagga
      661 tcaccagacc gctcgcccat aggtacggga aggagccggc ctcccagagt aggatcaggg
      721 cgtcttcgag caggaccgcg tccctaacgg tcgcgcccgg atagggcggt acgggggagg
      781 ggtcgacgat gacgcccgag gctccgtcgg ctagcacgac gcgccagtcg tcggtggcga
      841 cgcaggcgcg gtcgccgtat agatggcccc aggccggcgc ggcttggcct gggtcgccgc
      901 ggacgaaggc ccttcgcgag ccgtcgggca ggcgagcctc gagcaggacg ggcccgccaa
      961 tggcggcgac ggccgctcgc aacgcctcgg gcgctcgttc catgggttc
//
LOCUS       DAOOLN010001041         1009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_207695_length_1009_cov_2.579665, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001041 DAOOLN010000000
VERSION     DAOOLN010001041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207695_length_1009_cov_2.579665"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="PLW80_11870"
     CDS             complement(<1..846)
                     /locus_tag="PLW80_11870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB67255.1"
                     /translation="MLIPFVNRFLHWGAVGIVSPVLVLMAMSKGAPLEGVGVVMAAMS
                     IAAVALELPSGMLSDLLGRKRVYLASLAISLAGYALMVSARSMAAVCAAASIYGVARA
                     FSSGSIEALYIDSFIERRGKERLPALMTAMGVGETLGLAVGALAGGFIPMLWARLAPS
                     ADPYDGNLIAQAAIAVALFALTALSSPVEARRSGKATTLRSLAAGTAAVVRDSPVISR
                     LLAGAVFWGASFSAIEAYWQPRLGTVLGGAEASWLFGLVGALYFGAAIAGNALAGVLL
                     GRTGRV"
BASE COUNT          173 a          358 c          364 g          114 t
ORIGIN      
        1 gacgcggccc gtccgcccga gcaggacgcc cgccaaggcg ttcccggcga tggcggcgcc
       61 gaaatagagc gcgccgacga ggccgaacag ccagctggcc tcggcgccgc cgagcaccgt
      121 tccgaggcgc ggctgccaat acgcctcgat ggcgctgaac gacgcgcccc agaatacggc
      181 gccggccagg aggcgcgaga tgaccgggct atcgcggacg accgcggccg taccggcggc
      241 cagcgagcgt agcgtcgtcg cctttccgct ccggcgcgcc tcgacgggcg acgaaagggc
      301 cgttaaggcg aatagggcga cggcgatggc cgcctgcgcg atcaggttgc cgtcgtacgg
      361 atcggccgac ggggccaggc gcgcccagag catcgggatg aaaccgccgg cgagcgcccc
      421 gaccgcgagc ccgagcgtct cgccgacccc catcgccgtc atcagggccg gcaggcgttc
      481 cttccctcgc cgttctatga acgagtctat gtacagcgcc tcgatcgagc ccgacgagaa
      541 ggcgcgcgcg acgccgtaga tcgacgcggc cgcgcatacg gccgccatcg agcgagcgga
      601 taccatcagc gcgtacccgg ccagcgatat cgccagcgag gccaggtaca cgcgcttccg
      661 gcctagcagg tcggacagca tgccgctcgg cagctcgagc gcgacggcgg ctatcgacat
      721 ggcggccatg acgacgccga ctccctcgag cggggcgccc ttggacatgg ccatcagcac
      781 gagtacgggg ctgacgatcc cgacggctcc ccaatggagg aagcggttaa cgaagggtat
      841 cagcatccgg agccgccagg accgagccgc ggcagcagct ggagctgcgc gacgaccatg
      901 gccgcgccgg cggtagccgg cgcctcggga tcgcgacggc ggtagcgggc tatgacggcc
      961 tcgagctcgg cggccagtgc gcgtagctcg tcaggcgtca ggctgaggc
//
LOCUS       DAOOLN010001042         1008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_208083_length_1008_cov_2.205666, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001042 DAOOLN010000000
VERSION     DAOOLN010001042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208083_length_1008_cov_2.205666"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PLW80_11875"
     CDS             complement(<1..622)
                     /locus_tag="PLW80_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67256.1"
                     /translation="MSRRMMMLALLAFAASWLGAEPIDLSAHEAYVRNGFSLEWVFQA
                     PLPGEAPWVEAPGSPGDRPLSMRDLGLPDAPAHGVFSLERLKPSRYTVMIPFDADDEL
                     LASRTGVGLFLASVGQNWQIYLNGSLVRDEAFTSGDGTMRVERSVRGALVELDARYLR
                     RGRNQLSIMIAGDPRNAATGLSAGGPYLIDAYGTLESTRREGLKLML"
     gene            complement(699..>1008)
                     /locus_tag="PLW80_11880"
     CDS             complement(699..>1008)
                     /locus_tag="PLW80_11880"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF151 domain-containing protein"
                     /protein_id="HPB67257.1"
                     /translation="SDAIALAVRTTAEVFVSEDIVEMAGVPVDMIRESPPDEAPGSGG
                     LDTAPEADGSPIIEDVSVLRQGSPEFERAVLSAELENAIEAEDYERAARIRDRLKELH
                     "
BASE COUNT          157 a          343 c          357 g          151 t
ORIGIN      
        1 tcagcatcag cttaaggccc tcgcggcgcg tcgactcgag cgtgccgtag gcgtctatca
       61 ggtagggtcc gcccgcggac aggcccgtcg ccgcgttccg cgggtcgccg gctatcatga
      121 tggacagctg gttcctgccg cggcgcaggt agcgcgcgtc cagctcgacc agcgcgccgc
      181 gcaccgaccg ctcgacccgc atcgtgccgt cgccggaggt gaaggcctcg tcgcgcacga
      241 gcgagccgtt caggtagatc tgccagttct ggccgacgct cgccaggaag agcccgacgc
      301 cggtacgcga cgcgagcagc tcgtcgtcgg cgtcgaaggg tatcatcacg gtgtagcggc
      361 tcggcttgag gcgctcgagc gagaagaccc cgtgcgccgg ggcgtcgggc aggcccaggt
      421 cgcgcatcga cagcgggcgg tcgccgggcg agccgggcgc ctccacccag ggcgcctcgc
      481 cgggcagcgg cgcctggaat acccattcga gggagaagcc gttgcggacg taggcctcgt
      541 gggcgctcaa gtctatcggt tccgccccga gccatgacgc ggcaaaggcg agaagcgcga
      601 gcatcatcat tcgtcgtgac acatagaccc ccgggccatt gtatcgcgag tacggcgtat
      661 catggaagcg aaaaagcgac ggatagggcc gaccgggcct agtgcagctc cttgagccgg
      721 tcgcggatgc gcgcggcgcg ctcgtaatcc tccgcctcga tggcgttctc gagctcggcg
      781 ctgagcacgg ccctctcgaa ttcggggctg ccctgccgca gcaccgatac gtcctcgatg
      841 atcggggagc cgtcggcctc gggggcggta tcgaggcctc ccgagccggg agcctcgtcg
      901 ggcggggact cgcgtatcat atcgacgggg acgccggcca tctcgacgat gtcctcggag
      961 acgaacacct ccgcggtcgt cctgaccgcc agggctatcg cgtcggac
//
LOCUS       DAOOLN010001043         1008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_208095_length_1008_cov_2.153200, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001043 DAOOLN010000000
VERSION     DAOOLN010001043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208095_length_1008_cov_2.153200"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PLW80_11885"
     CDS             <1..487
                     /locus_tag="PLW80_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HPB67258.1"
                     /translation="QKVLQGLLREQVSIRNLVAILETLADYAGITKDPGFLVEKSRQS
                     LGRQICLQYADQDKTMRVLTIEPSLEQRIIDARVDTPAGAMSALEPATQRQWIKALTL
                     AVTAVQKQGHFPVILCSEAARALVKSSTEREIPDLVVLSVPEIVADIQVEAIGEIRVE
                     G"
     gene            491..>1008
                     /locus_tag="PLW80_11890"
     CDS             491..>1008
                     /locus_tag="PLW80_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HPB67259.1"
                     /translation="MQYLVEQGSSHREVLEKVRARYGDAAQVLSHRTVRSGGFLGLFA
                     REGVEITFYLKDDAVREGEKKKADIEEEKRRLLERVNQERAIQEVLSEVRSLKDTLAE
                     SVARPAAQDGEHPTLAALDALLVDNDFSPWYRAELLARARDEFSLGELEDYAAVEEAF
                     LEWIGDTVRIPAP"
BASE COUNT          197 a          330 c          326 g          155 t
ORIGIN      
        1 ccagaaggtg cttcagggcc tcctgcgcga gcaggtatcg atacgcaacc ttgtcgccat
       61 actcgagacc ctcgccgact acgcaggtat cacgaaggat ccgggcttcc tcgtcgagaa
      121 gtccaggcag tccctgggcc gccagatttg cctccagtac gccgaccagg acaagacgat
      181 gcgcgtgctg accatagagc cctcactcga gcagcgtatc atcgacgcgc gggttgacac
      241 gcccgccggc gcgatgtcgg ccctggagcc ggcgacccag cgccaatgga tcaaggcctt
      301 gacgctcgcc gtcaccgcgg tgcagaaaca ggggcatttc cccgtgatcc tctgctccga
      361 ggcggccagg gccctcgtca agtcgagcac ggagcgggag atacccgacc tcgtcgtgct
      421 atcggtgccg gagatcgtgg ccgacatcca ggtagaggcc atcggagaga tacgcgttga
      481 aggataaatt atgcaatacc tggtagagca gggctccagt catcgggagg tcctggagaa
      541 ggtccgcgcc cgctacggcg acgcggccca ggtcctgagc catcgtaccg tacgatccgg
      601 cggcttcctc ggcctgttcg cccgcgaggg cgtcgagata accttctacc tgaaggacga
      661 cgcggtgcgc gagggcgaaa agaagaaggc cgacatcgag gaggagaagc gcaggctgct
      721 cgagcgcgtc aatcaggagc gggccatcca ggaggtactc tccgaggtgc gctcgctcaa
      781 ggatacgctc gccgagagcg tcgcgaggcc cgcggcccag gacggcgagc atccgacgct
      841 cgccgccctc gacgccctgc tcgtcgacaa tgatttctcg ccctggtacc gcgccgagct
      901 cctggccagg gcccgggacg aattcagcct cggcgaactc gaggactacg cggcggtcga
      961 agaggccttc ctcgaatgga taggcgatac cgtacgtatc cccgcgcc
//
LOCUS       DAOOLN010001044         1007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_208284_length_1007_cov_4.069328, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001044 DAOOLN010000000
VERSION     DAOOLN010001044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208284_length_1007_cov_4.069328"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..>1007)
                     /locus_tag="PLW80_11895"
     CDS             complement(162..>1007)
                     /locus_tag="PLW80_11895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HPB67260.1"
                     /translation="ELPAEAFAAFARKNGLEAEILRCAAAAGELASLPLFDCIGSMES
                     LYGLAKASRRAESGGGALDAEAPDDELLVLVSGEASLTYGGIAFENLTPGAALGDERT
                     LCRSMRLFSAVGRGPTAWLAFPRAVVETRPALLWRLREGLERRLAIVKAVFEFRWRPE
                     YELGITAMDEQHRRIFGAMDELTGAAENDATLVLERLEDLLADHFSDEEALQAAVGYP
                     GREEHAREHAKLLSELSEFMERIRTGVVATGLSNFMKDCFIRHTLLEDRKYAPWVHDG
                     DPRRS"
BASE COUNT          176 a          347 c          328 g          156 t
ORIGIN      
        1 ggttggggcg ctttcccgag acgaggccgc tggcggagaa gaaggcctcg aagcccaggc
       61 ccaggatgtc tacggtctcg gtcatcgcgc gagcatagcg tatatgggta tcccgggtca
      121 tccggccgcg gcggacgaag cccggcggaa acggccggcg ttcacgacct gcgcggatcc
      181 ccgtcgtgta cccaaggcgc gtacttgcga tcctcgagta gcgtatggcg gatgaagcaa
      241 tccttcatga aattcgacaa gccagtggcg acgacgcccg tacggatacg ctccatgaat
      301 tcggatagct cgctgagaag cttcgcgtgc tcgcgggcgt gctcctcgcg gccggggtag
      361 ccgaccgccg cctgtagcgc ctcctcgtcg gagaaatggt cagccagcag atcctcgaga
      421 cgttccagga ccaaggtcgc gtcgttttcc gccgcgccag tcagctcgtc catcgccccg
      481 aagatgcggc gatgttgctc gtccatagcg gtaatgccga gctcgtactc gggtctccag
      541 cggaactcga agaccgcctt gacgatggcc agccggcgct cgagcccctc gcggagccgc
      601 cacagtagcg ccggcctcgt ctccacgacg gcgcgcggga acgccagcca ggccgtaggc
      661 ccgcgcccaa cggccgaaaa cagccgcatc gatctgcaga gcgtacgttc gtcgccgagc
      721 gccgcgcccg gggtcaggtt ctcgaaggcg atgccgccgt aggtcagcga ggcctcgccg
      781 gagacgagca cgagtaactc gtcgtccggc gcctcggcat cgagggctcc gccgcccgac
      841 tctgcacggc gggaagcctt cgccagccca tagaggcttt ccatcgagcc gatacagtcg
      901 aaaagcggca gcgaggccag ctcacccgcg gcggccgcac agcgtagtat ctcggcctcg
      961 aggccgttct tcctcgcgaa cgccgcgaac gcctccgccg gtagttc
//
LOCUS       DAOOLN010001045         1006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_208638_length_1006_cov_4.089380, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001045 DAOOLN010000000
VERSION     DAOOLN010001045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208638_length_1006_cov_4.089380"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="PLW80_11900"
     CDS             <1..751
                     /locus_tag="PLW80_11900"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPB67261.1"
                     /translation="VERSRLELTVQARTADLSAANDDLVREIGDRERAESKAVAMAAE
                     KAMLLRELQHRVKNSLTIIVSLIGLESSKSGSAELRESLESLESRVSALAALYDILYD
                     TGGIERLPLAAYLDRVVDAAAESLGADEKGISLTRDIAPIAMDMKRAVSLGLIVNELV
                     TDCLKYAFPGGARGTVRVSLRAEAGRYLLVVEDDGVGLPEGFDPDSVDGFGFKLVGML
                     ARQLHGEFSWSSRDGSRFSLSIPAEDQASSG"
BASE COUNT          160 a          349 c          336 g          161 t
ORIGIN      
        1 cgtcgagcgc tccaggctcg agctgaccgt ccaggcccgc accgccgacc tgtccgcggc
       61 gaacgacgac ctggtccgcg agatcggtga ccgcgagcgc gccgagtcca aggccgtggc
      121 catggcggcg gagaaggcga tgctgctccg cgagctacag catcgcgtca agaacagcct
      181 taccattatt gtcagcctca tagggctcga gtcgagcaag tccggctcgg ccgagctgcg
      241 cgagtcgttg gagtcgctgg agtcgagggt atcggcgctc gcggccctct acgacattct
      301 ctacgacacc ggcggcatcg aacgcctgcc cctggcggcc tacctcgacc gtgtcgtcga
      361 cgccgcggcc gagagtctcg gggccgacga gaagggtatc agcctgacga gggacatcgc
      421 gcccatcgcg atggacatga agcgtgccgt ctcgctcggg ctcatcgtca atgagctcgt
      481 gaccgactgc ctcaagtacg ccttccccgg cggggctcga ggaacggtac gagtcagcct
      541 acgggccgag gccggccgct acctgctcgt cgtcgaggac gacggcgtcg ggttgcccga
      601 gggcttcgac cccgactcgg tcgacggctt cggcttcaag ctcgtcggga tgctggccag
      661 gcagctccat ggcgagttct cctggtccag ccgcgacggc tcccggttct cgctctccat
      721 accggccgaa gaccaagcct cctcgggatg acgacgaaac gcatggccgc tcgaccggtt
      781 ttcgggatat acttagaagc gcggcgccgc cgtactgcac ggaacggcgc tagccggcca
      841 tcggggaggt gaggcctcgc tcgcgccccg gcgtcgagcc gccgagcggc cgcctcgaaa
      901 gcgtatgaaa cgatccaccg cgttaaccgt cgccatcttc gcggccttct ccgccttcgg
      961 gcagggctcg ggtccggcca caggcgagac cgtcggggtg gccttc
//
LOCUS       DAOOLN010001046         1004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_209369_length_1004_cov_3.060063, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001046 DAOOLN010000000
VERSION     DAOOLN010001046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209369_length_1004_cov_3.060063"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLW80_11905"
     CDS             <1..244
                     /locus_tag="PLW80_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPB67262.1"
                     /translation="IEAARRLGVEPGACLVLEDSPYGVAAAKKAGMRCVALPDPAAGL
                     SGFDAADLVVDGGAARLDPEAVMARYSWVAPSRTAE"
     gene            complement(378..824)
                     /locus_tag="PLW80_11910"
     CDS             complement(378..824)
                     /locus_tag="PLW80_11910"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPB67263.1"
                     /translation="MSHESALSKVSLFANLEPKYIKGIAQISTERDFAPGDHLMKQGE
                     DGIGLFIILSGKVKIEKVNANGQSVEIASNGPGDVMGELAVLDGAKRTATVTAVEPTK
                     CLVLASWEFNSFMKSHPEVGTAILPIVVRRFRETNDALVGLSGVKA"
BASE COUNT          154 a          330 c          332 g          188 t
ORIGIN      
        1 catcgaggcg gccaggcggc tcggtgtcga acccggcgcc tgcctcgtgc tcgaggactc
       61 gccgtacggc gtggcggccg ccaagaaggc gggtatgcgc tgcgtggcct tgccggaccc
      121 ggcggctggc ctgtcgggct tcgacgcggc cgacctcgtc gtcgacgggg gcgccgccag
      181 gctcgacccg gaagccgtga tggcgcgcta ttcgtgggtc gcgccttcgc gtacagctga
      241 atagcgcgca agcttcgtat caacgccccg gaacgggcca tgcacaaggt tcgtaccacc
      301 cgggatgata aaaaaacagg cggcttccgt catgaagccg cccgttccgt acctggccga
      361 tcgtcggccc cgcgcgctta ggccttgacg cctgacagcc ctacgagggc gtcgttcgtt
      421 tcacggaagc gtcgcacgac tatgggcagg atggccgtgc cgacctcggg gtggctcttc
      481 atgaaggagt tgaactccca cgaggccagc acgaggcact tggtcggctc gaccgcggta
      541 accgtggcgg tgcgcttggc gccgtcgagc acggccagct cgcccatcac gtcgccgggg
      601 ccgttcgagg ctatctcgac cgactggccg ttggcgttca ccttttcgat cttgaccttg
      661 cccgacagga tgatgaacag gccgatgcca tcctcgccct gcttcatcag gtgatcgccg
      721 ggggcgaagt cgcgctcggt cgagatctgc gctatgccct tgatatactt cggctcgagg
      781 ttggcgaaca ggctgacctt cgagagcgcg ctttcatgac tcatccagta cctccgtata
      841 aggccgctac gctacggtgc gcggcttatc tcaagatacg cctaggtcgg cggaccgtca
      901 acctgaagtc tccggttaac ggggctacgg ttcgactatg aaccgttgtt tccgcgcggg
      961 cctccggccg cggcgcgccg ggctccaggc ttcttgggcg cggc
//
LOCUS       DAOOLN010001047         1004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_209520_length_1004_cov_1.894626, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001047 DAOOLN010000000
VERSION     DAOOLN010001047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209520_length_1004_cov_1.894626"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="PLW80_11915"
     CDS             complement(<1..933)
                     /locus_tag="PLW80_11915"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPB67264.1"
                     /translation="MGDHITSGRRNEPVRVALLIALAAVVFVAYSSHLFYLQVIRGGE
                     YQKKAVTIARKIERLPAQRGEIYDRNYNLPLVLNVDSFALVVVPAEIPADLRDTVFAK
                     LASVLEEPIDEIRRRIPPSYYRLFQQIEVASSVGQDVIVRISERIDEFPGVSWYSRPK
                     RNYLETGSFSHIIGYVGDISRDELKLYYNRGYQAGDIIGKAGIERQYDSLLRGTDGRQ
                     YKTVDVRGRRVDAELRDVDPPMMGKNVVLTIDRATQILAEKSLGERIGSIVVLKPATG
                     EILALVSYPSFNANLIVEKGGNNEYARLLNDPRTP"
BASE COUNT          183 a          294 c          333 g          194 t
ORIGIN      
        1 cggcgtcctc gggtcgttga gcagcctggc gtactcgttg ttgccgccct tctctactat
       61 gaggttggca ttgaaggacg gataggagac gagggcgagt atctccccgg tggccggctt
      121 caggacgacg atggagccga tgcgctctcc gagcgacttc tcggccagta tctgagtggc
      181 gcggtcgatg gtcaagacga cgttcttgcc catcatcggc gggtcgacgt cgcgaagctc
      241 agcgtcgacg cggcgcccgc gcacgtcgac ggtcttgtac tggcgtccat cggtaccgcg
      301 cagcaggctg tcgtactgac gctcgatgcc ggccttgccg atgatgtctc cggcctgata
      361 accgcggttg tagtatagct tgagctcatc gcgcgatatg tcgccgacat agcctatgat
      421 atgggagaag ctacctgtct ccaggtaatt gcgcttgggc cgcgagtacc acgagacgcc
      481 gggaaactcg tcgatgcgct cggatatgcg gacgatcacg tcctggccca cggacgatgc
      541 tacctctatc tgctggaaca gcctgtagta cgagggcggg atacgccgcc tgatctcgtc
      601 tatcggctct tcgagcacgg acgcgagctt ggcgaagacg gtatcgcgca ggtccgccgg
      661 aatctcggcg ggaacgacga cgagcgcgaa cgagtcgacg ttcaacacga gcggcaggtt
      721 gtagttgcgg tcgtatatct cgccgcgctg ggccggcaag cgctcgatct tcctggctat
      781 cgtgacggcc ttcttctggt actcgccgcc gcgtatgacc tgcaggtaga agaggtgcga
      841 cgagtacgcg acgaacacga cggccgcgag ggctatcagc aacgctacgc ggacgggctc
      901 gttccggcgg cccgaggtga tatggtcgcc catactatcg ggcgtcccgg ggcacgatga
      961 agcggtcgag gaatccgagt agcatgaaca tgaacggcgc gaca
//
LOCUS       DAOOLN010001048         1003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_209697_length_1003_cov_2.941983, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001048 DAOOLN010000000
VERSION     DAOOLN010001048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209697_length_1003_cov_2.941983"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..454)
                     /locus_tag="PLW80_11920"
     CDS             complement(83..454)
                     /locus_tag="PLW80_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67265.1"
                     /translation="MGTTGVQDGPVAAEMPEEQKSIFSDQPPVVCSICGKQIFDLSSA
                     LAGRDGGQPVHFDCALASVSEGERLEPTERIAYIGRGSFAVVEYRDKSMTAFTVKRRL
                     QWEKEGEKYDWRRAIQQRIGL"
     gene            complement(516..680)
                     /locus_tag="PLW80_11925"
     CDS             complement(516..680)
                     /locus_tag="PLW80_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67266.1"
                     /translation="MSSETNREEHAERLEELKAMVHDESYMSGAILRIANVLSDEIMD
                     GIGVSYGGWK"
     gene            complement(677..>1003)
                     /locus_tag="PLW80_11930"
     CDS             complement(677..>1003)
                     /locus_tag="PLW80_11930"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPB67267.1"
                     /translation="EGIVSASLLASGWDVVARNWKRGRGELDIVAFRDGVLAVVEVKD
                     IDAFGLESVEASVGGRKRARIVETSKLFIHSHREFNSAAVRYDVATVAAGALADYFEN
                     AFSERT"
BASE COUNT          172 a          348 c          271 g          212 t
ORIGIN      
        1 gaagcctcgg ccatacatcc tccgctacca gtatgcgcgt ttcaggcgct ttggccagat
       61 cgggaaaggc gcgaacccgc ccttataggc ctatgcgctg ctggatcgcc ctccgccagt
      121 cgtacttctc gccttctttt tcccactgga ggcggcgctt gacggtgaag gcggtcatac
      181 tcttgtcccg atactcgacg acggcgaacg agcctcgacc gatatacgct atgcgctcgg
      241 tcggctcgag gcgctccccc tcggagacgc tggccaaggc gcagtcgaaa tggaccggct
      301 gcccgccgtc ccgccccgcc agggcgctcg agaggtcgaa aatctgtttg ccgcatatgg
      361 agcatacgac cggaggctgg tcggagaaga tagacttctg ttcctccggc atctcggccg
      421 cgactggccc gtcctgcacc ccggtagtcc ccacgccctt agcgccggat cttccgcgcc
      481 cccgccctct cctgccgaac cgtcgcttcg agccgttatt tccatccgcc atagcttact
      541 ccgataccgt ccatgatttc atcgctcagc acgttggcta tacgaagtat ggccccgctc
      601 atgtaggact catcatgcac catggccttg agttcttcaa gccgttcggc gtgttcttcc
      661 ctgttcgttt cgctactcat gtacgctccg agaaagcgtt ttcgaagtaa tcggccaagg
      721 cgccggccgc cacggtggct acgtcgtaac ggacggccgc gctattaaat tctcgatggg
      781 agtggatgaa gagtttagac gtttcgacga tcctggctcg ttttctcccg ccgaccgaag
      841 cctcgacgct ctcgaggccg aaagcgtcga tatccttgac ctcgacgacg gcgagcaccc
      901 cgtcccggaa ggcgacgata tcgagctcgc ctcgtccgcg cttccagtta cgcgccacca
      961 cgtcccagcc gctcgccagt agcgacgccg atacgatccc ctc
//
LOCUS       DAOOLN010001049         1002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_210120_length_1002_cov_2.054910, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001049 DAOOLN010000000
VERSION     DAOOLN010001049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210120_length_1002_cov_2.054910"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PLW80_11935"
     CDS             complement(<1..517)
                     /locus_tag="PLW80_11935"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HPB67268.1"
                     /translation="MNERIEAVASRLRQLEDLVSDPGLSRDPKRYKDAMRERSRLAEI
                     VEAYDGVRRIQAEIAGTKAMLHDEADPELKELARDELRGLEDGLEAAEKRVSELLVPR
                     DPLDDKNTIMEIRAGTGGDEAALFVADLYRMYTRYADEKGWKIEVMSSSETGLGGFKE
                     IILSISGDQVFG"
     gene            complement(514..693)
                     /locus_tag="PLW80_11940"
     CDS             complement(514..693)
                     /locus_tag="PLW80_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67269.1"
                     /translation="MKLDMNPRGNGACPLCVSHGSCRVQRKLIENLSLVGPDDGEGME
                     IVIYSCPYFKEKIAP"
BASE COUNT          189 a          321 c          310 g          182 t
ORIGIN      
        1 tgccgaagac ctggtctccg gagatcgaca ggatgatctc cttgaaaccg ccgaggccgg
       61 tctccgagct ggacataact tcgatcttcc agcccttctc gtccgcgtag cgcgtataca
      121 tacggtagag gtccgccacg aacagggcgg cctcgtcgcc gccggtaccg gcgcggatct
      181 ccataatggt gttcttatcg tcgagcgggt cgcgaggtac aagtagctcc gaaacacgct
      241 tctcggccgc ctcgaggccg tcctcgaggc cacgcaactc atcacgggcc agttccttga
      301 gctcggggtc cgcctcgtcg tgcagcatgg ccttggtgcc cgctatctcg gcctggatac
      361 gccttacgcc gtcataggcc tcgacgatct cggccaggcg ggagcgctcg cgcatggcgt
      421 ccttataacg cttggggtcg cgcgataggc ctggatcgga gacgaggtct tccagctgcc
      481 taaggcggga cgcgaccgct tcgattcgct cgttcacggc gcgatctttt ccttgaaata
      541 cgggcaggag tagatgacga tttccatgcc ttcgccgtcg tcagggccga cgaggctcag
      601 gttctcaatc agcttgcgct ggacgcggca cgacccgtgc gatacgcaca gggggcaggc
      661 gccgttgccg cgggggttca tatcgagctt catggcgcta gggtacaaaa aaaacgcttg
      721 gaatacaaga cgcgagccgg acggcgcgcg ggaccgttga cgcgacgacc cggttgccta
      781 cctgccgcgc cgcaggatac tatgcggcat gggccgagtc gacgtagccg gaatcacgaa
      841 accttcctcg tcatcctcct ccagtcgagg ctcctccgcc gaccggccgg gcggggcgag
      901 cgatagccgt tcctaaggcc ggacgagcgc gccggcgcaa gccacgccaa gaccgacatc
      961 cagaccaagg agaacagcat gctcgacttt aaatatatca ag
//
LOCUS       DAOOLN010001050         1002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_210189_length_1002_cov_1.658923, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001050 DAOOLN010000000
VERSION     DAOOLN010001050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210189_length_1002_cov_1.658923"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PLW80_11945"
     CDS             complement(<1..305)
                     /locus_tag="PLW80_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HPB67270.1"
                     /translation="MNTITIDAPVSDDAELVILESIYSSQKRSRALTQRDLAEAAGLS
                     LGMTNALVKRFSDKGWVLLKRLNSRNIQYALTPDGVAEIARRTYRYFKRTARAAGLYR
                     "
     gene            complement(744..>1002)
                     /locus_tag="PLW80_11950"
     CDS             complement(744..>1002)
                     /locus_tag="PLW80_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB67271.1"
                     /translation="YMLYGEDFADWFSGGLYTNVLLGHRGSGTTGSDGPSYDPVEYLG
                     LGVGIEMVFLRHLSTSVEFMYVGSYPLALEFGFGGSLKYRF"
BASE COUNT          203 a          291 c          327 g          181 t
ORIGIN      
        1 cggtagagcc cggccgctcg cgcggtccgc ttgaagtagc gatacgtgcg cctggctatc
       61 tccgccacgc cgtcgggcgt cagcgcgtac tggatgttgc gcgaattcag gcgcttgagc
      121 agcacccatc ccttgtcgga gaagcgcttg acgagggcgt tggtcatccc gagcgacagc
      181 ccggcggctt ccgccaggtc gcgctgggtc agcgcgcggg agcgtttctg cgaggagtag
      241 atgctctcga ggatgacgag ctcggcgtcg tccgagaccg gcgcgtctat ggtaatggta
      301 ttcatgcgag ttatattagc gggagctatg cccggcgtac gggaagacct tccgcctgcg
      361 gaaaggcgga tacgataggg aacctgacgt tccgcggcgg ctcggggcgg ccgaaacacg
      421 ctcccgatcg acggcggaat cctactcgga ccgacccggt aacgccacgc ggcattcccg
      481 gaatcgtcag cccttgttca ttcctcgaac aatcctagca cgggacgtgc tggaattcaa
      541 tacgcaatcg agccgatcaa ctatgagcct acttggggac agaggtttga tacggagttg
      601 cgccatggcg ttctggggac agaggttcga actagaaatg ctctctagta ctctctgggg
      661 acagaggttc attaagaaag ccccgcggcg caacagacgc gccgcggggc ttatattcaa
      721 aagatcgggg acggagcttg gcgctagaag cggtatttca ggcttccgcc gaagccgaat
      781 tcgagggcca gggggtacga tccgacgtac atgaactcga cgctcgtcga caggtgccta
      841 aggaagacca tctcgatgcc gacgccgagt ccgaggtact cgacgggatc gtaggacggg
      901 ccgtccgagc cggtcgtccc cgagccgcga tggcccagaa gcacgttcgt atacaggccg
      961 cctgagaacc agtcggcgaa atcctcgccg tagagcatgt ac
//
LOCUS       DAOOLN010001051         1001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2700
            NODE_210368_length_1001_cov_2.659619, whole genome shotgun
            sequence.
ACCESSION   DAOOLN010001051 DAOOLN010000000
VERSION     DAOOLN010001051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864775
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,393
            CDSs (total)                      :: 2,375
            Genes (coding)                    :: 2,366
            CDSs (with protein)               :: 2,366
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210368_length_1001_cov_2.659619"
                     /isolate="AMR_MDS_2700"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PLW80_11955"
     CDS             <1..217
                     /locus_tag="PLW80_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPB67272.1"
                     /translation="TDPAGPWQSNWELSTARALGVLHYLSDFGVPEARFQVSGLAATM
                     PIADDSTAEGRAYNRRVDIIILDEGHL"
     gene            237..791
                     /locus_tag="PLW80_11960"
     CDS             237..791
                     /locus_tag="PLW80_11960"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HPB67273.1"
                     /translation="MSDEDFDKAGPDFSDSPEIADKPKGKGGGKAILGLLKWIGIGLG
                     AVIFIIVIVVVTVSLLNRQAKPMTTVPTSEEYQRATPIWATFTSIDQITTSTIDKEPW
                     SVMIKLNLAYDQADKEIQTELTSRKYQIQDALRNFFSMKSIKELMPNREQELKEELRE
                     MLNRMLSKPAIKDVYFQTFSRNQM"
     gene            869..>1001
                     /locus_tag="PLW80_11965"
     CDS             869..>1001
                     /locus_tag="PLW80_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HPB67274.1"
                     /translation="MTEVLSQDEIDQLLTAINAGDTSLETVQSATDSRKIKIYDFRRP
                     "
BASE COUNT          231 a          293 c          303 g          174 t
ORIGIN      
        1 gacggacccg gctggtccct ggcagtcgaa ctgggaatta tcgacggcga gggccctcgg
       61 cgtgctacac tacctgagcg acttcggggt gcctgaggcg cgtttccagg tgtcgggcct
      121 cgcggcgacg atgccgatag ccgacgattc tacagcggag ggccgggcgt acaaccggcg
      181 cgttgacatt ataatactgg acgagggcca tctatgatga ccggaggtag cgctatatga
      241 gcgacgaaga tttcgacaag gccggaccgg atttcagcga ctccccggag atcgcggaca
      301 agccgaaggg caagggcggc ggcaaggcga tactcggcct gctcaagtgg atcggtatag
      361 gcctcggcgc ggtcatcttc atcatcgtca tagtcgtcgt caccgtgagt ctcctcaatc
      421 ggcaggccaa gcctatgacg accgtgccga cctcggagga ataccagcgc gccaccccga
      481 tctgggccac cttcacgtcg atcgaccaga tcacgacgag cacgatcgac aaggagccct
      541 ggtcggtcat gatcaagctg aacttggcct acgaccaggc cgacaaggaa atccagaccg
      601 agctgacctc gcgcaagtac cagatccagg atgcgctgcg caatttcttc agtatgaaga
      661 gcatcaagga gctaatgccc aaccgcgagc aggagctcaa ggaggagcta cgggagatgc
      721 tcaaccgcat gctttcgaag ccagcgatca aggacgtgta tttccagacc ttctctcgta
      781 atcagatgta ggcgttcgac gtcgcggatg ggcccgcgac gttgctaaaa gcgtttttct
      841 atgcaatgat agcaggcgga gtgaacagat gaccgaagtc ctgtcccagg acgagataga
      901 ccagctcctc accgccatca acgccggcga tacgtcgctc gagacggtac agtcggcgac
      961 cgactcgcgc aagatcaaga tctacgactt caggcgcccg g
//
